LOCUS       DAEEDS010000001        89421 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEEDS010000001 DAEEDS010000000
VERSION     DAEEDS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89421
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.001"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(86..469)
                     /gene="rplQ"
                     /locus_tag="KLH32_000001"
     CDS             complement(86..469)
                     /gene="rplQ"
                     /locus_tag="KLH32_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBE0137012.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(510..1499)
                     /gene="rpoA"
                     /locus_tag="KLH32_000002"
     CDS             complement(510..1499)
                     /gene="rpoA"
                     /locus_tag="KLH32_000002"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBE0137013.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(1525..2145)
                     /gene="rpsD"
                     /locus_tag="KLH32_000003"
     CDS             complement(1525..2145)
                     /gene="rpsD"
                     /locus_tag="KLH32_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBE0137014.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(2179..2568)
                     /gene="rpsK"
                     /locus_tag="KLH32_000004"
     CDS             complement(2179..2568)
                     /gene="rpsK"
                     /locus_tag="KLH32_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBE0137015.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2585..2941)
                     /gene="rpsM"
                     /locus_tag="KLH32_000005"
     CDS             complement(2585..2941)
                     /gene="rpsM"
                     /locus_tag="KLH32_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBE0137016.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3088..3204)
                     /gene="rpmJ"
                     /locus_tag="KLH32_000006"
     CDS             complement(3088..3204)
                     /gene="rpmJ"
                     /locus_tag="KLH32_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBE0137017.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(3236..4567)
                     /gene="secY"
                     /locus_tag="KLH32_000007"
     CDS             complement(3236..4567)
                     /gene="secY"
                     /locus_tag="KLH32_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBE0137018.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(4575..5009)
                     /gene="rplO"
                     /locus_tag="KLH32_000008"
     CDS             complement(4575..5009)
                     /gene="rplO"
                     /locus_tag="KLH32_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBE0137019.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(5013..5192)
                     /gene="rpmD"
                     /locus_tag="KLH32_000009"
     CDS             complement(5013..5192)
                     /gene="rpmD"
                     /locus_tag="KLH32_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBE0137020.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(5196..5699)
                     /gene="rpsE"
                     /locus_tag="KLH32_000010"
     CDS             complement(5196..5699)
                     /gene="rpsE"
                     /locus_tag="KLH32_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBE0137021.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(5714..6067)
                     /gene="rplR"
                     /locus_tag="KLH32_000011"
     CDS             complement(5714..6067)
                     /gene="rplR"
                     /locus_tag="KLH32_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBE0137022.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(6077..6610)
                     /gene="rplF"
                     /locus_tag="KLH32_000012"
     CDS             complement(6077..6610)
                     /gene="rplF"
                     /locus_tag="KLH32_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBE0137023.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(6623..7015)
                     /gene="rpsH"
                     /locus_tag="KLH32_000013"
     CDS             complement(6623..7015)
                     /gene="rpsH"
                     /locus_tag="KLH32_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBE0137024.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(7049..7354)
                     /gene="rpsN"
                     /locus_tag="KLH32_000014"
     CDS             complement(7049..7354)
                     /gene="rpsN"
                     /locus_tag="KLH32_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBE0137025.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7369..7908)
                     /gene="rplE"
                     /locus_tag="KLH32_000015"
     CDS             complement(7369..7908)
                     /gene="rplE"
                     /locus_tag="KLH32_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBE0137026.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(7923..8237)
                     /gene="rplX"
                     /locus_tag="KLH32_000016"
     CDS             complement(7923..8237)
                     /gene="rplX"
                     /locus_tag="KLH32_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBE0137027.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(8248..8619)
                     /gene="rplN"
                     /locus_tag="KLH32_000017"
     CDS             complement(8248..8619)
                     /gene="rplN"
                     /locus_tag="KLH32_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBE0137028.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(8784..9038)
                     /gene="rpsQ"
                     /locus_tag="KLH32_000018"
     CDS             complement(8784..9038)
                     /gene="rpsQ"
                     /locus_tag="KLH32_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBE0137029.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(9038..9229)
                     /gene="rpmC"
                     /locus_tag="KLH32_000019"
     CDS             complement(9038..9229)
                     /gene="rpmC"
                     /locus_tag="KLH32_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBE0137030.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(9229..9639)
                     /gene="rplP"
                     /locus_tag="KLH32_000020"
     CDS             complement(9229..9639)
                     /gene="rplP"
                     /locus_tag="KLH32_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBE0137031.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(9652..10353)
                     /gene="rpsC"
                     /locus_tag="KLH32_000021"
     CDS             complement(9652..10353)
                     /gene="rpsC"
                     /locus_tag="KLH32_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBE0137032.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(10371..10703)
                     /gene="rplV"
                     /locus_tag="KLH32_000022"
     CDS             complement(10371..10703)
                     /gene="rplV"
                     /locus_tag="KLH32_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBE0137033.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(10718..10996)
                     /gene="rpsS"
                     /locus_tag="KLH32_000023"
     CDS             complement(10718..10996)
                     /gene="rpsS"
                     /locus_tag="KLH32_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBE0137034.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(11013..11834)
                     /gene="rplB"
                     /locus_tag="KLH32_000024"
     CDS             complement(11013..11834)
                     /gene="rplB"
                     /locus_tag="KLH32_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBE0137035.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(11852..12154)
                     /gene="rplW"
                     /locus_tag="KLH32_000025"
     CDS             complement(11852..12154)
                     /gene="rplW"
                     /locus_tag="KLH32_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBE0137036.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(12151..12756)
                     /gene="rplD"
                     /locus_tag="KLH32_000026"
     CDS             complement(12151..12756)
                     /gene="rplD"
                     /locus_tag="KLH32_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBE0137037.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(12767..13396)
                     /gene="rplC"
                     /locus_tag="KLH32_000027"
     CDS             complement(12767..13396)
                     /gene="rplC"
                     /locus_tag="KLH32_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBE0137038.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(13429..13740)
                     /gene="rpsJ"
                     /locus_tag="KLH32_000028"
     CDS             complement(13429..13740)
                     /gene="rpsJ"
                     /locus_tag="KLH32_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBE0137039.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            14121..14588
                     /locus_tag="KLH32_000029"
     CDS             14121..14588
                     /locus_tag="KLH32_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE0137040.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="KLH32_000030"
     CDS             complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="KLH32_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBE0137041.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="KLH32_000031"
     CDS             complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="KLH32_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBE0137042.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="KLH32_000032"
     CDS             complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="KLH32_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0137043.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LG"
     gene            complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="KLH32_000033"
     CDS             complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="KLH32_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBE0137044.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="KLH32_000034"
     CDS             complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="KLH32_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBE0137045.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="KLH32_000035"
     CDS             complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="KLH32_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBE0137046.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="KLH32_000036"
     CDS             complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="KLH32_000036"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBE0137047.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="KLH32_000037"
     CDS             complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="KLH32_000037"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBE0137048.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="KLH32_000038"
     CDS             complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="KLH32_000038"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBE0137049.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(21084..21806)
                     /locus_tag="KLH32_000039"
     CDS             complement(21084..21806)
                     /locus_tag="KLH32_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0137050.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="KLH32_000040"
     CDS             complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="KLH32_000040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0137051.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            23006..23224
                     /locus_tag="KLH32_000041"
     CDS             23006..23224
                     /locus_tag="KLH32_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBE0137052.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="KLH32_000042"
     CDS             complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="KLH32_000042"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE0137053.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(23958..24158)
                     /locus_tag="KLH32_000043"
     CDS             complement(23958..24158)
                     /locus_tag="KLH32_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBE0137054.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(24168..25905)
                     /gene="kefB"
                     /locus_tag="KLH32_000044"
                     /pseudo
     CDS             complement(24168..25905)
                     /gene="kefB"
                     /locus_tag="KLH32_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(25905..26456)
                     /gene="kefG"
                     /locus_tag="KLH32_000045"
     CDS             complement(25905..26456)
                     /gene="kefG"
                     /locus_tag="KLH32_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBE0137055.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            26587..28500
                     /locus_tag="KLH32_000046"
     CDS             26587..28500
                     /locus_tag="KLH32_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0137056.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            28500..29522
                     /locus_tag="KLH32_000047"
     CDS             28500..29522
                     /locus_tag="KLH32_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0137057.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            29516..29734
                     /locus_tag="KLH32_000048"
     CDS             29516..29734
                     /locus_tag="KLH32_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBE0137058.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            29788..30657
                     /locus_tag="KLH32_000049"
     CDS             29788..30657
                     /locus_tag="KLH32_000049"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBE0137059.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(30712..31116)
                     /locus_tag="KLH32_000050"
     CDS             complement(30712..31116)
                     /locus_tag="KLH32_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBE0137060.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            31418..32050
                     /gene="crp"
                     /locus_tag="KLH32_000051"
     CDS             31418..32050
                     /gene="crp"
                     /locus_tag="KLH32_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBE0137061.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            32101..34191
                     /locus_tag="KLH32_000052"
     CDS             32101..34191
                     /locus_tag="KLH32_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE0137062.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(34258..35478)
                     /gene="argD/dapC"
                     /locus_tag="KLH32_000053"
     CDS             complement(34258..35478)
                     /gene="argD/dapC"
                     /locus_tag="KLH32_000053"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBE0137063.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(35564..36127)
                     /gene="pabA"
                     /locus_tag="KLH32_000054"
     CDS             complement(35564..36127)
                     /gene="pabA"
                     /locus_tag="KLH32_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBE0137064.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(36159..36761)
                     /locus_tag="KLH32_000055"
     CDS             complement(36159..36761)
                     /locus_tag="KLH32_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBE0137065.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(36751..36918)
                     /locus_tag="KLH32_000056"
     CDS             complement(36751..36918)
                     /locus_tag="KLH32_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBE0137066.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(37023..37604)
                     /gene="ppiA"
                     /locus_tag="KLH32_000057"
                     /gene_synonym="rotA"
     CDS             complement(37023..37604)
                     /gene="ppiA"
                     /locus_tag="KLH32_000057"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBE0137067.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            37875..39056
                     /gene="tsgA"
                     /locus_tag="KLH32_000058"
     CDS             37875..39056
                     /gene="tsgA"
                     /locus_tag="KLH32_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBE0137068.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            39318..41861
                     /gene="nirB"
                     /locus_tag="KLH32_000059"
     CDS             39318..41861
                     /gene="nirB"
                     /locus_tag="KLH32_000059"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBE0137069.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            41858..42184
                     /gene="nirD"
                     /locus_tag="KLH32_000060"
     CDS             41858..42184
                     /gene="nirD"
                     /locus_tag="KLH32_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBE0137070.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            42310..43116
                     /gene="nirC"
                     /locus_tag="KLH32_000061"
     CDS             42310..43116
                     /gene="nirC"
                     /locus_tag="KLH32_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBE0137071.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            43135..44508
                     /gene="cobA"
                     /locus_tag="KLH32_000062"
     CDS             43135..44508
                     /gene="cobA"
                     /locus_tag="KLH32_000062"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE0137072.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            44755..44922
                     /locus_tag="KLH32_000063"
     CDS             44755..44922
                     /locus_tag="KLH32_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBE0137073.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            45217..46554
                     /gene="frlA"
                     /locus_tag="KLH32_000064"
                     /pseudo
     CDS             45217..46554
                     /gene="frlA"
                     /locus_tag="KLH32_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            46575..47597
                     /gene="frlB"
                     /locus_tag="KLH32_000065"
     CDS             46575..47597
                     /gene="frlB"
                     /locus_tag="KLH32_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBE0137074.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            47647..48477
                     /gene="frlC"
                     /locus_tag="KLH32_000066"
     CDS             47647..48477
                     /gene="frlC"
                     /locus_tag="KLH32_000066"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBE0137075.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            48474..49259
                     /gene="frlD"
                     /locus_tag="KLH32_000067"
     CDS             48474..49259
                     /gene="frlD"
                     /locus_tag="KLH32_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBE0137076.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            49359..50090
                     /gene="phoB"
                     /locus_tag="KLH32_000068"
     CDS             49359..50090
                     /gene="phoB"
                     /locus_tag="KLH32_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBE0137077.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(50250..51335)
                     /locus_tag="KLH32_000069"
     CDS             complement(50250..51335)
                     /locus_tag="KLH32_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBE0137078.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(51347..52651)
                     /locus_tag="KLH32_000070"
     CDS             complement(51347..52651)
                     /locus_tag="KLH32_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBE0137079.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(52663..53016)
                     /locus_tag="KLH32_000071"
     CDS             complement(52663..53016)
                     /locus_tag="KLH32_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBE0137080.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(53027..53905)
                     /locus_tag="KLH32_000072"
     CDS             complement(53027..53905)
                     /locus_tag="KLH32_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBE0137081.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(53902..55128)
                     /locus_tag="KLH32_000073"
     CDS             complement(53902..55128)
                     /locus_tag="KLH32_000073"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE0137082.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(55129..56292)
                     /locus_tag="KLH32_000074"
     CDS             complement(55129..56292)
                     /locus_tag="KLH32_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBE0137083.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(56376..56738)
                     /locus_tag="KLH32_000075"
     CDS             complement(56376..56738)
                     /locus_tag="KLH32_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE0137084.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(56755..57660)
                     /locus_tag="KLH32_000076"
     CDS             complement(56755..57660)
                     /locus_tag="KLH32_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137085.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(57930..58934)
                     /gene="trpS"
                     /locus_tag="KLH32_000077"
     CDS             complement(57930..58934)
                     /gene="trpS"
                     /locus_tag="KLH32_000077"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBE0137086.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(58927..59685)
                     /gene="gph"
                     /locus_tag="KLH32_000078"
     CDS             complement(58927..59685)
                     /gene="gph"
                     /locus_tag="KLH32_000078"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBE0137087.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(59678..60355)
                     /gene="rpe"
                     /locus_tag="KLH32_000079"
     CDS             complement(59678..60355)
                     /gene="rpe"
                     /locus_tag="KLH32_000079"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBE0137088.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(60373..61209)
                     /gene="dam"
                     /locus_tag="KLH32_000080"
     CDS             complement(60373..61209)
                     /gene="dam"
                     /locus_tag="KLH32_000080"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE0137089.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(61316..62602)
                     /gene="damX"
                     /locus_tag="KLH32_000081"
     CDS             complement(61316..62602)
                     /gene="damX"
                     /locus_tag="KLH32_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBE0137090.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(62694..63782)
                     /gene="aroB"
                     /locus_tag="KLH32_000082"
     CDS             complement(62694..63782)
                     /gene="aroB"
                     /locus_tag="KLH32_000082"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBE0137091.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(63839..64360)
                     /gene="aroK"
                     /locus_tag="KLH32_000083"
     CDS             complement(63839..64360)
                     /gene="aroK"
                     /locus_tag="KLH32_000083"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBE0137092.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(64761..65999)
                     /gene="hofQ"
                     /locus_tag="KLH32_000084"
     CDS             complement(64761..65999)
                     /gene="hofQ"
                     /locus_tag="KLH32_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBE0137093.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(65911..66315)
                     /gene="hofP"
                     /locus_tag="KLH32_000085"
     CDS             complement(65911..66315)
                     /gene="hofP"
                     /locus_tag="KLH32_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBE0137094.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(66305..66745)
                     /gene="hofO"
                     /locus_tag="KLH32_000086"
     CDS             complement(66305..66745)
                     /gene="hofO"
                     /locus_tag="KLH32_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBE0137095.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(66729..67268)
                     /gene="hofN"
                     /locus_tag="KLH32_000087"
     CDS             complement(66729..67268)
                     /gene="hofN"
                     /locus_tag="KLH32_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBE0137096.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(67268..68047)
                     /gene="hofM"
                     /locus_tag="KLH32_000088"
     CDS             complement(67268..68047)
                     /gene="hofM"
                     /locus_tag="KLH32_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBE0137097.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            68167..70719
                     /gene="mrcA"
                     /locus_tag="KLH32_000089"
     CDS             68167..70719
                     /gene="mrcA"
                     /locus_tag="KLH32_000089"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBE0137098.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(70884..71444)
                     /gene="nudE"
                     /locus_tag="KLH32_000090"
     CDS             complement(70884..71444)
                     /gene="nudE"
                     /locus_tag="KLH32_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBE0137099.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            71764..73899
                     /gene="igaA"
                     /locus_tag="KLH32_000091"
     CDS             71764..73899
                     /gene="igaA"
                     /locus_tag="KLH32_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBE0137100.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            73964..74632
                     /gene="yrfG"
                     /locus_tag="KLH32_000092"
     CDS             73964..74632
                     /gene="yrfG"
                     /locus_tag="KLH32_000092"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBE0137101.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            74643..75044
                     /gene="hslR"
                     /locus_tag="KLH32_000093"
     CDS             74643..75044
                     /gene="hslR"
                     /locus_tag="KLH32_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBE0137102.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            75069..75938
                     /gene="hslO"
                     /locus_tag="KLH32_000094"
     CDS             75069..75938
                     /gene="hslO"
                     /locus_tag="KLH32_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBE0137103.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(76082..77806)
                     /locus_tag="KLH32_000095"
     CDS             complement(76082..77806)
                     /locus_tag="KLH32_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBE0137104.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            78185..79807
                     /gene="pckA"
                     /locus_tag="KLH32_000096"
     CDS             78185..79807
                     /gene="pckA"
                     /locus_tag="KLH32_000096"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBE0137105.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(79883..81235)
                     /gene="envZ"
                     /locus_tag="KLH32_000097"
     CDS             complement(79883..81235)
                     /gene="envZ"
                     /locus_tag="KLH32_000097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBE0137106.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(81232..81951)
                     /gene="ompR"
                     /locus_tag="KLH32_000098"
     CDS             complement(81232..81951)
                     /gene="ompR"
                     /locus_tag="KLH32_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBE0137107.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            82179..82655
                     /gene="greB"
                     /locus_tag="KLH32_000099"
     CDS             82179..82655
                     /gene="greB"
                     /locus_tag="KLH32_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBE0137108.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            82752..85073
                     /locus_tag="KLH32_000100"
     CDS             82752..85073
                     /locus_tag="KLH32_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBE0137109.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            85511..85738
                     /gene="feoA"
                     /locus_tag="KLH32_000101"
     CDS             85511..85738
                     /gene="feoA"
                     /locus_tag="KLH32_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBE0137110.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            85755..88076
                     /gene="feoB"
                     /locus_tag="KLH32_000102"
     CDS             85755..88076
                     /gene="feoB"
                     /locus_tag="KLH32_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBE0137111.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            88076..88312
                     /gene="feoC"
                     /locus_tag="KLH32_000103"
     CDS             88076..88312
                     /gene="feoC"
                     /locus_tag="KLH32_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBE0137112.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            88515..89402
                     /gene="rpnA"
                     /locus_tag="KLH32_000104"
     CDS             88515..89402
                     /gene="rpnA"
                     /locus_tag="KLH32_000104"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBE0137113.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        21540 a        24170 c        22341 g        21370 t
ORIGIN      
        1 aaaagagtac agctattgta gataagtggg gatactacgg gtataaaaaa acccgccggg
       61 gcgggttttt ttacattgct tcagattact ctgcagcagc ttctgctttc tctgaacgat
      121 caaccagctc gatgtaagcc atcggcgcgt tgtcgcctgc acggaagcca cacttcagaa
      181 tacgagtgta accaccggca cggctcgcga aacgcgggcc cagttcgtta aacagttttg
      241 ccacgatctc gttatcacga gtacgggcga atgccagacg acgattagca acgctatcag
      301 tcttggcaag agtaatcagc ggctcaacta cgcggcgcag ctctttcgct ttaggcagag
      361 tcgtcttgat gatttcatga cgaaccagtg aacctgccat attgcggaac atagcctggc
      421 gatggctgct gttgcggttc agttgacgac cactcttacg atggcgcatg accttatcct
      481 tctcagtaaa accttaacct gtgatccggt tactcgtcag cgatgcttgc cggtggccag
      541 ttttccaagc gcatgcccag agacagtcca cgggaagcca gcacgtcttt aatctcagta
      601 agagattttt taccaaggtt aggcgtttta aggagctcaa cctcggtacg ctgtaccaga
      661 tcaccgatat agtggatagc ttctgcttta aggcagttag cagagcggac agtcaattcc
      721 agatcgtcaa cagggcgcag caggatcgga tcgaactctg gtttctcttc tttcacttca
      781 ggctgacgta catcacgtaa gtcaacgaaa gcttccagtt gttcagccag aatggttgcc
      841 gcacgacgaa tcgcctcttc aggatcgatt gtgccgttgg tttccatttc gatgaccagc
      901 ttgtccaggt cggtacgctg ttctacacgc gctgcttcaa cattgtaggc aatacgctcc
      961 acagggctgt agcatgcgtc gaccagcaga cggccgattg ggcgctcatc ttcttccgaa
     1021 tgaattcggg tagaagccgg cacataacca cgaccgcgct gaactttgat acgcatgcta
     1081 atagacgcgt tctcatcggt caggtggcag atcacgtgct gcggcttgac gatttcgaca
     1141 tcaccgtcgt gggtgatatc ggctgcagtc acagggccaa tgccagattt attcaaggta
     1201 agaataactt catctttgcc ctgaactctc accgccagcc ctttcaggtt gagcaggatt
     1261 tccaggatat cttcctgaac gccttctttg gtgctgtact catgtagtac accatcaatc
     1321 tcaacctcgg tcaccgcgca acccggcatc gatgagagca gaatacggcg cagtgcgtta
     1381 cccagagtat ggccaaagcc acgctctaaa ggctcaaggg tcaccttggc gtgcgtcgaa
     1441 ctcacttgct cgatatcaac caggcgcggt tttagaaact ctgtcacaga accctgcatt
     1501 gtgtcctctc tttggtacta agctttactt ggagtaaagc tcgacgatca ggtgttcgtt
     1561 aatgtccgca gacagatcag aacgctccgg cttacgctta aacgtacctt ccatcttgcc
     1621 agcatcaact tccagccaag ttggcttttc acgctgctca gccagctcca gagcggcttt
     1681 cacgcgagac tgcttcttcg ctttctcacg aatgcttaca acgtcattcg gactaacctg
     1741 ataagaagcg atgttaacaa cacgaccgtt taccataatt gctttatggc taaccagctg
     1801 acgtgcttct gcacgagtgg caccgaagcc catacggtat acaacgttgt ccagacgacc
     1861 ttccagcaga gccaacaggt tttcaccggt gttgcctttc agacgtgctg cttctttgta
     1921 gtagttacgg aactgacgct ccagcacacc atagatacgg cgaacttttt gcttttcacg
     1981 caactgcaca ccatagtcag acagacgcgg tttacgcgca ccgtgctggc caggagcttg
     2041 ttcaatttta cacttggtat cgatcgcgcg aacgccagac ttaaggaata agtcggtgcc
     2101 ctcacgacgg ctcagcttga gcttaggacc caaatatctt gccattttct ttctccaaca
     2161 aacctggaaa acgaggcgtt atacgcgacg ttttttcggc ggacgacaac cgttatgagg
     2221 gatcggagtc acatcagtaa tgttagtgat gcggaaacct gcggcgttca gagcacgaat
     2281 agtagattcg cggcctggac ccggaccttt aaccataact tccagattct tgatgccgta
     2341 ttctttcacg gcgtcagcgc aacgctctgc tgcaacctga gctgcaaacg gagtggattt
     2401 gcgagaacca cggaaaccgg aaccaccggc tgttgcccaa cccaacgcgt taccctgacg
     2461 atcagtgata gtcacgatgg tgttgttgaa agaagcatgg atatgagcca cgccgtcaga
     2521 gacttgtttt cttacacgtt tacgtgcacg aattggtgcc tttgccatta ttcaatcacc
     2581 ccgattattt cttgatcggt ttgcgcggac ccttacgggt acgtgcgttg gtcttggtac
     2641 gctgaccgcg aaccgggaga ccacgacgat gacgcaaacc gcgatagcaa ccaagatcca
     2701 tcaggcgctt gatgctcatg ctgatttcac ggcgcagatc accttcaacg acaaatttgg
     2761 caacttcgtc acgcagcgtg tcgatttgtc cttcagacag ctcactgatc ttaacatctt
     2821 cagcgatacc cgctgcagcc aggatggctt tagaacgggt cttgccgacg ccataaatcg
     2881 aagttaatgc gattacggca tgcttatgat caggaatgtt aatgcctgct atacgggcca
     2941 ctatgcactc ctactattta atatgtacgt tccatgctga aaagcccgtt ttcaggatac
     3001 tcaaatggaa acgtacagac atacaaaaga ttggctggct aatctagcca gctcaaccca
     3061 actttgcaag aaaaatatgc gaaaaaatca gccttggcgc tgtttatgct tcggctcggc
     3121 actgcaaatc acacggatga caccatcacg cttaacgatt ttgcagttac ggcataattt
     3181 cttgacggaa gcacgaactt tcatttttac tctccgtaac ttctcgggcg accaattatc
     3241 ggccgtagcc tttcaggttc gccttcttca atgcagactc atactgactg gacatcatca
     3301 gagtttgcac ttgagccata aagtccataa tcacgacaac aacgataagc agtgaggtcc
     3361 caccgaagta gaacggtact ttcattgcat cacgcatgaa ctccgggatc aggcagataa
     3421 aggtaatata cagcgcacca accaaggtca ggcgggtcat tactttatcg atatacttcg
     3481 ccgtttgctc tcccggacga attcctggta caaatgcacc ggacttcttc aggttatctg
     3541 ctgtttcacg cgggttgaaa accaacgccg tgtagaagaa acagaagaag atgattgcag
     3601 acgcatagag taacacataa agcggttgcc caggctgcaa atacagcgaa attgttgtca
     3661 gccagttcca accagtaccg cccccgaacc atgacgcgat ggtcgccggg aacagaataa
     3721 tactggaagc gaagattgcc gggattaccc ccgccatatt cactttcagc ggtaaatgtg
     3781 tgctctgtgc agcatagaca cgacgacctt gctgacgttt cgcgtagttt accacaatgc
     3841 ggcgttgacc acgctcaaca aacacaacaa agaacgtcac tgcaaatact aatactgcaa
     3901 ccaacagcaa cacgaggaag tgcaggtcgc cttgacgcgc ttgctcgata gtatgggcaa
     3961 tggctggcgg gagtcccgcg acaataccgg cgaagataat gattgaaata ccgttgccga
     4021 tacctcgttc agtgatctgt tcgcccaacc acatcaggaa catcgttcct gtgaccagac
     4081 ttacaacagc ggtgaagtag aatgcaaagc ccgggttaat caccaggcct tgcataccag
     4141 gcatattcgg cagaccggta gcaataccga tcgactggaa tattgccagc accagagtac
     4201 cgtaacgggt gtactggctg atcttacgac gaccagactc cccttctttc ttaatttctg
     4261 ccaacgttgg gtgaaccacc gtcagcagct ggataatgat cgacgccgaa atatacggca
     4321 tgatccccag agcaaagata gaagcacggc tgagagcacc accagagaac atgttaaaca
     4381 tctcaatgat ggtgcctcgc tgttgctcaa gcagtttggc aagtacagcg gcatcaatac
     4441 cagggatcgg aataaaagag ccaatacgga acacaatcag cgcaccgata acaaacagca
     4501 gtctgcgttt cagctcgcct aagccacctt tggcactttg aaaatctaat cccggttgtt
     4561 tagccatctg ctacttattc ctcgatttta ccgccagcag cttcgatagc agcacgagcg
     4621 cctttagtaa cacgcaggcc acgaacagtt accggagtcg ttacttcgcc agccaggatc
     4681 actttcgcga actcgatctg gataccgata atgttagccg ctttcagcgt gttcaggtct
     4741 acaacaccgc cttctacttt agccaggtca gatagacgaa cttcggctgt aatcgctgct
     4801 ttacgagaag tgaagccgaa tttcggcaga cgacggtaca gaggcatctg accaccctcg
     4861 aaaccgcgac gtacgccacc gccagaacga gacttctgac ctttgtgacc acgaccaccg
     4921 gttttaccga ggccagaacc gataccacga cccaggcgtt tacccgcctt tttggagcct
     4981 tcggccggag acagagtatt taaacgcatc tcttactcct caactttaac catgaaggaa
     5041 accgcgttga tcataccgcg aatagcagga gtatcctcgc gctctacggt gtgaccaata
     5101 cgacgcagac ccaagccaag cagcgttgcc ttgtgtttcg gcagacgacc gattgcactg
     5161 cgggtttgag taattttaat agtctttgcc atggtttatt tccccagaat ttcttcaacg
     5221 gatttaccac gcttggcagc gaccatttct ggagaattca tattttccag gccatcaata
     5281 gttgcacgaa ccacgttgat cgggttggtg gaaccatatg ctttagccag aacgttatga
     5341 accccagcga cttccagaac ggcgcgcatt gcaccaccgg cgatgatacc ggtaccttcg
     5401 gaagccggct gcatgaatac gcgagaaccc gtgtgaacac ctttaacagg gtgttgtaga
     5461 gtgccgttat tcagcgcgac gttaatcata ttgcgacggg ctttttccat cgctttctgg
     5521 atcgctgctg gaacttcacg cgctttaccg taaccaaaac caacgcgacc gttaccatcg
     5581 ccaactacag tcagagctgt gaaggagaaa atacgaccac ctttaacggt tttagatacg
     5641 cggtttaccg cgatcagctt ttcctgcagt tcgccagctt gtttttcgat gtgagccatc
     5701 ttacacctct accttagaac tgaaggccag cttcacgggc agcatctgcc agtgcctgga
     5761 cacgaccatg atattggaac ccggaacggt caaaggatac atctttgatg cctttttcca
     5821 gagcgcgttc agcgacagct ttacccacag ctgcagccgc gtctttgtta ccggtgtact
     5881 tcagttgttc agcgatagct ttttctacag tagaagcagc taccagaact tcagaaccgt
     5941 tcggtgcaat tacctgtgcg taaatgtgac gcggggtacg atgtaccacc aggcgagttg
     6001 cgcccagctc ctggagcttg cggcgtgcgc gggtcgcacg acggatacga gcagatttct
     6061 tatccatagt gttaccttac ttcttcttag cctctttggt acgcacgact tcgtcggcgt
     6121 aacgaacacc cttgcctttg taaggctcag gacgacggta ggcgcgcaga tccgctgcaa
     6181 cctggccgat cacctgctta tcagcgcctt tcagcacgat ttcagtctga gtcggacatt
     6241 cagcagtgat acccgcaggc agctgatggt caacaggatg agagaaaccc agagacaggt
     6301 taatcacatt gcctttaacc gctgcacggt aacctacacc aaccagctgc agcttcttag
     6361 tgaagccttc ggtaacaccg ataaccattg agttcagcag ggcacgcgcg gtaccagcct
     6421 gtgcccaacc gtctgcgtaa ccatcacgcg gaccgaaggt cagggtatta tctgcatgtt
     6481 taacttcaac agcatcgttg agagtacgag tcagctcgcc gtttttacct ttgatcgtaa
     6541 taacctgacc gttgattttt acgtcaacgc cggcaggaac aacgaccggt gctttagcaa
     6601 cacgagacat tttttcctcc gattaggcta cgtagcagat aatttcgcca ccaagaccag
     6661 cctggcgcgc tgcacgatca gtcataacac ctttagaggt agaaacaact gcgataccca
     6721 gacccgccat aactttcggc agctcatctt tacgtttata gatgcgcaga cctgggcggc
     6781 tgacacgctg aatgctttct acaacagctt tgccctggaa atacttcaga gtaagttcca
     6841 gttcaggctt ggtgtcgcct tcaactttaa aatcttcaat aaaaccttct tccttcagca
     6901 cgttggcgat tgccactttc agcttggagg aaggcatggt gaccgcagct ttgttcgcgg
     6961 cctgaccgtt acggatacgg gtcagcatat ccgcgatcgg atcttgcatg ctcatctgtc
     7021 tttactcccg tgattcaatt ggtgacaatt accagctagc ctttttcaga cccgggattt
     7081 caccgcgcat agcggcttca cggaccttaa tacggctcaa cccgaacttc cgcaggaaac
     7141 catgcggacg acctgtttga cggcagcggt tacgctgacg agacgggctg gaatcacgcg
     7201 gcagagtctg cagcttgaga acagcgttcc aacgatcttc gtcggaagcg ttcacatcag
     7261 agatgatcgc tttcagttca gcgcgtttcg cgaagtattt atcagctaaa gctacgcgtt
     7321 ttacttcgcg tgctttcatt gattgcttag ccatttagta accctacctt acttgcggaa
     7381 cgggaagtca aaggcagcca gcagagcgcg gccttcttcg tcagatttcg cagtagtggt
     7441 aatggtaata tccaaaccac gaacgcggtc gactttatcg tagtcgattt ctgggaagat
     7501 gatctgctca cggacaccca tgctgtagtt accacgaccg tcgaaagact tagcggacag
     7561 gccacggaag tcacggatac gaggtacagc aatagtgatc aggcgctcaa agaactccca
     7621 catgcgttcg ccacgcagag ttactttaca gccgatcgga tagccctgac ggattttgaa
     7681 gcctgcaaca gatttgcgtg ctttggtgat cagcggtttt tgaccggaga ttgctgccag
     7741 gtctgctgct gcgttatcca gcagtttttt gtcagcgatc gcttcaccaa cacccatgtt
     7801 cagggtgatc ttctcgaccc gagggacttg catgacagaa ttgtagttaa actcagtcat
     7861 gagtttttta actacttcgt ctttgtagta atcatgcagt ttcgccatcg tactactcca
     7921 aattacttga tagtttcgct gttagacttg aagaaacgga cttttttgcc gtcttcgaat
     7981 ctaaagccta cacggtcagc cttgccggtt gccgcattga agattgctac gttggaaacc
     8041 tgaatagcgg cttctttttc aacgatgcca cccggttggt tcagggccgg aaccggcttc
     8101 tgatgtttct taaccaggtt gataccttca acaatgacct tgccggaaga caggacattc
     8161 ttaactttac cgcgtttacc tttatcttta ccggttaaca cgataacttc gtcatcacga
     8221 cggattttcg ctgccatgat tcgctcctta gagtacttct ggtgccagag agataatttt
     8281 catgaacttc tcactacgaa gctcacgagt taccggccca aaaatacgcg taccgatagg
     8341 ctgctcgctg ttgttgttca gaagaacaca agcattacca tcgaagcgaa tgacagaacc
     8401 gtccgggcga cgaacaccct tcttggtgcg caccactacc gccttcagca catcaccttt
     8461 tttgacctta ccacgcggaa ttgcttcttt gatggtgatc ttgatgatgt cgcctacgcc
     8521 tgcgtagcga cggtgcgagc cacccagaac cttgatacac attacgcgac gtgcaccgga
     8581 gttgtcggcg acgttcagca tagtctgttc ttggatcatt ttagtgctcc gctaatgtca
     8641 actactactg agacccgaaa atcaggtcgt taaaaatccc catatcgagg gcgcggcatt
     8701 ataacaccgc ttcaaggata tgggtagaaa aaataaacgg ctcatttctg agccgtttat
     8761 tcgtatcgag agagtgtact gtattacaga accgctttct ctacaacgcg aaccagcgtc
     8821 caggatttag tcttggacag cggacggcat tcgcggattt caaccacgtc accgataccg
     8881 cattcgttgt tctcgtcatg tacgtgcagt ttggtcgtac gcttgatgaa tttaccgtag
     8941 atcgggtgtt tcacaaaacg ttcgatagca acaacaatgg atttctccat tttgtcgcta
     9001 acaacgcgac cttgcagagt acggatttta tcggtcatta cgcacccgcc ttctcgttca
     9061 gtaaagtctt aacgcgtgcg acatcgcgac gcacttgctt caacaggtga gactgttgca
     9121 gctggccact tgcagcctgc atacgcaggt tgaactgctc acgcagcagg ttcagcagct
     9181 cggtgttcag ctcttcaatg ctcttctcac gcagctcttt tgctttcatt acatcaccgt
     9241 cttagttaca aaggtggttt taatcggcag tttcgctgct gccagcttga atgcttcacg
     9301 ggccagctct tccggaacac cgtccatttc atacaggact ttacccggct gaatcaaggc
     9361 aacccaatac tccacgttac ctttaccttt acccatacgc actgccagcg gcttttcagt
     9421 gatcggtttg tccgggaaca cacggatcca gatcttacct tgacgcttaa ctgcacgggt
     9481 catagcacga cgtgctgctt cgatctgacg ggcagtcaga cgaccacggc caacagcttt
     9541 cagaccgaag ctgccgaagc taacatccgt accctgcgcc agaccgcggt tacggccttt
     9601 gtgcatttta cggaattttg tacgctttgg ttgtaacatc agcgacgctc cttatttacg
     9661 gcctttacgc tgctgctttt taggctgagc agccggtttt tccggttgtt caacagcagc
     9721 cataccaccc aggatctcgc ctttgaagat ccacacttta acgccgatta caccgtaagt
     9781 ggtgtgcgct tcagaggtgt tgtagtcgat gtcagcacgc agagtgtgca gcggtacgcg
     9841 accttcgcgg taccattcgg tacgtgcgat ttccgcgccg cccagacggc cgctaacttc
     9901 aactttaata cctttagcgc ccagacgcat tgcgttctgt acagcacgct tcatagcacg
     9961 acggaacata acgcgacgtt ccagctgaga agtgatgctg tcagcaacca gttttgcgtc
    10021 cagttcaggc ttacgaactt cggcgatgtt gatctgtgca ggaacgccag cgatgtccgc
    10081 tacgacctta cgcagttttt ctacgtcttc acctttttta ccgataacga tacccgggcg
    10141 agcagtgtga atggttacac ggatgctctt agccggacgc tcgataacga tacgagatac
    10201 ggacgcttta gccagttcct tagtcaggta ctgacgtact ttaaaatcgc tgtccaggtt
    10261 gtcagcgaat tctttggtgt tcgcaaacca agtagagttc catggtttta caatacccaa
    10321 gcgaatacca ttaggatgta ctttctgacc cattgctagt ctccagagtc tcagcgatcg
    10381 gacacaacca cagtgatgtg gctggtgcgc ttcaggatgc gatctgcacg accttttgca
    10441 cgcggcataa tgcgcttcat gctcgggcct tcgtctacga aaattttcgt aactttcaga
    10501 tcgtcaatgt cagcgccatc gttgtgttca gcgttagcaa tggcagattc cagaactttc
    10561 ttgacctgta cagccgcttt cttgttggtg taggtcaaaa tatccagagc ctgcgacact
    10621 ttcttaccgc gaatcaggtc agcaacaagg cgaaccttct gagcagaaga acgagcatgg
    10681 cgatgtttag caatagtttc catctcttcc tcctacctta tttcttcttc gcttttttat
    10741 cagcagcgtg gccgcgataa gtacgagtcg gtgcgaattc acccagtttg tgaccgacca
    10801 tttcgtcggt tacaaatacc ggaacgtgct gacgaccatt atggacagcg atggtcaaac
    10861 cgatcatgtt aggaaagatc gttgaacgac gggaccaagt gcgcaggggc ttcttgtctc
    10921 cgctttccac cgctttctct accttcttca gcaagtgcag gtcaataaaa ggacctttct
    10981 tgagagaacg tggcatggct tatcctctaa aattatttgc tacggcgacg tacgatgaat
    11041 ttatcagtac gcttgttgct gcgggtcttc ttacctttgg tctgaacgcc ccacggagtt
    11101 accgggtgct taccaaagtt acgaccttca ccaccaccat gtgggtggtc taccgggttc
    11161 atcgcggtac cgcgaacggt cggacgaaca ccacgccagc gtgcagcacc tgctttaccc
    11221 agaacgcgca gcatatgctc agcattgcca acttcgccca gagttgcacg gcagtctgct
    11281 tctactttac gcatttcacc agaacgcaga cgcagggtga cataagcacc atcacgagca
    11341 acgatctgaa cgtaagtacc agcggaacgt gccagctgac cgcctttacc tggtttcatt
    11401 tctacgttat gaacagtaga accaaccggg atgttgcgca tcggcagggt gttacctggt
    11461 ttgattgcag catcaacgcc agactgaatc tggtcgccag ctttcaggcc tttaggggcc
    11521 aggatgtaac ggcgttcacc gtctttgtac agaaccagcg cgatgttcgc ggaacggttc
    11581 ggatcgtact caagacgttc aacaactgcc gggataccgt ctttgttgcg tttgaagtca
    11641 acaatacggt aagcctgctt gtggccacca ccgatatgac gagtggtgat acggccattg
    11701 ttgttacgac caccggattt gctgtttttt tccagcaacg gagcaaaagg tttgcccttg
    11761 tgcagctcag ggttaaccac tttaactacg tggcgacgac ccggagatgt cggtttacat
    11821 ttaacaactg ccattgtatt actcctccga cttactcagc gccgccaacg aagtccagat
    11881 tctggccttc tttcagggtg acgtaagctt ttttccagtc gctacgacga ccgatacgct
    11941 gtccgtgacg tttaactttc cctttaacta ccagggtgtt aacgacttcg acttcgactt
    12001 caaacagttt ctgcacagca gctttgattt ctgctttggt cgcgtctttc gcaactttga
    12061 gtacgatggt gttggatttt tccatcgcag tagacgcttt ttcagaaacg tgcggtgcac
    12121 gcagcacctt cagcagacgt tcttcacgaa tcatgccagc atctcctcaa cttgcttaac
    12181 agcatcagca gtcattacga ctttgtcgaa ggcgatcagg ctaaccgggt cgataccagt
    12241 tgcatcgcgt acgtcaacct tctgcaggtt gcgcgcagcc aggaacaggt tttcgtccag
    12301 ctcaccggtg atgatcagca catcttccag agccatgtct ttcagtttct gtgccagcag
    12361 cttagttttc ggcgcttcta cagagaactt ctcgacaacg atcagacgat cctgacgtac
    12421 cagttcggac aggatgcttt tcagcgcgcc gcggtacatc ttcttgttaa ctttttgact
    12481 gtggtcctgc ggacgagcag caaaggtcac gccaccagaa cgccagatcg ggctcttgat
    12541 agaaccagaa cgcgcacggc cggtgccttt ctggcgccac ggttttttac cggaaccagt
    12601 tacttcagca cgagtcttct gagcacgagt accctgacga gcaccagctg cataagcaac
    12661 aacaacctgg tgaaccagcg cttcgttgaa atcacgaccg aaggtagttt cggaaacagt
    12721 cagcgcgctc tgcgcgtctt tcaatactaa ttccattgct atctccttac gccttcacag
    12781 ctggtttaac gatcaggtcg ctaccggttg cacccgggac agcaccttta accagcagca
    12841 ggttgcgctc agcgtcaacg cgtactacgt caaggctctg aacggttaca cgttcgttac
    12901 ccatctgacc tgccattttc ttgcctttga acactttgcc cggagtctgg ttctgaccga
    12961 tagaacccgg aacgcggtga gacaaggagt taccgtgagt agcgtcctgg gtacggaagt
    13021 tccagcgctt aacggtacct gcgaaacctt tacctttaga ggtgccagtt acgtcaactt
    13081 ttttaacgtc agcaaacagt tcaacgctaa tgctctgacc tacagtgaac tcttcgcctt
    13141 cagccaggcg gaattcccac agaccacggc cagcttctac gccagcttta gcgaagtggc
    13201 cagcttcagg cttggtcaca cggttagctt ttttagcacc ggtggtcacc tgaatagcac
    13261 ggtagccatc gttagccagg tctttaacct gagtaacgcg gtttgcttca acttcgatta
    13321 cggttactgg gatagaaacg ccgtcttctg tgaagatacg ggtcataccc acttttttac
    13381 cgactaaacc aatcattgtt tcaacctctc aatcgctcaa tgacctgatt aacccaggct
    13441 gatctgcacg tctacaccgg cagccagatc cagacgcatc agagcatcaa cggttttctc
    13501 ggttggctca acgatgtcaa ccagacgcaa gtgagtacgg atttcgtact gatcgcgcgc
    13561 gtctttgttg acgtgcgggg agatcagaac agtgaagcgc tctttgcgtg tcggcagcgg
    13621 gatcggacca cggacctgcg caccagtgcg cttggcagtc tcgacgattt ccgcggttgc
    13681 ttgatcgatc agacgatgat caaacgcttt caggcggata cggattcttt ggttctgcat
    13741 gagaccagag ctccaattat tttataaacg aaaatgatta ctcctcagac ccattacgat
    13801 tgatgggaga gtgtaaccgt tcttacgtag ctccccgatt gggagcattg ttaggtagcc
    13861 aaattcggct aactgaggtt cagattgaac ctgctgtcaa ctacgacaag cccgcgcatt
    13921 atacgcaaat ctgagcctga cgcaagcatc gggtagaaat taatcgcaac gcgcagattg
    13981 cgagctgcat tccacgaaat ttgtaaggtt gtgaagatag cgtttttcac cggaatcagg
    14041 ttaacaaatc agcgctctct ctctgttgct gagcatccgg agttattcga tggtgatact
    14101 ccaattacag aggatgtttt atggacacta cccttccctt tctaattttg tacgcttgtt
    14161 tatcagccct actttttttc tgggatgcaa agcatggctt gttacctgac agatttacgt
    14221 gcccgttact ctggtcaggt cttttgttct atcaagtttg tcatcctgat ggtttagccg
    14281 atgcgttatg gggggcaatt gttggttacg gcacattcgc tgtcatttac tgggggtatc
    14341 gcatactgcg acataaagaa ggattaggct atggtgatgt gaagtttctc gccgcccttg
    14401 gcgcctggca ttcatgggcc ttcttaccac gattggtttt ccttgctgcc tcattcgctt
    14461 gcggagccgt agttattggt ctgctcatga gaggaaaaga atcattaaaa aacccgctgc
    14521 cttttgggcc atttctggcg gctgcgggtt tcgttatagg ctgggatagt ttgctgatag
    14581 gccgttaatc tttaacttta atttgtgatt gcaggtaatt ttgtaggccg attttaccga
    14641 tgagatccag ttcagtttcg agccagtcga tatgaccttc ttcatcagca aggatctcga
    14701 tcatcatatc gcggctaaca tagtcatgaa cgctgtcggc atatgcgata gcctcacgca
    14761 gatccttcgc gccttccagt tctaatcgca ggtcagattg cagcatctcc tcgacatctt
    14821 caccaatccc cagtttgcct aaatcctgta agttggggat tccttctaaa aacaaaatac
    14881 gctcgatata tttatcagcg tgtttcatct catcgatgga ttcatggtat tcgacgtcat
    14941 tgagacgcat caggccccag tttttaaaca tccgggcatg gagaaaatac tgattgattg
    15001 cgacaagctc atttcccaat agtttattga gataatttat gattttaaca tcacctttca
    15061 ttttatagtc cctccgcttc cactattaga gcgtagatgg ggctagcagg atgtcaaaaa
    15121 ataagcgtgg gcttcaggca atctctttaa attctggcat ctgcatcaac tcgtcctgca
    15181 tcacttcgcg cgcggcgcga atgcacttac cgcattgatt tcccacagga ataaacttac
    15241 gcaattgttg gaaagattgc ggatgaaact gacggactgc ctggcgaatt tttttgtcac
    15301 ttacaccatt acacaaacaa acgtacatga ttgctcccgt tcaaattctg cacaaagtgt
    15361 aaatgagaat ggttatgatt acaatagtac agttttattt gcgccatagt tcagcgagca
    15421 aaattacgac taaatatttc gagctcaaat tacgggcagc aaaaagggcg ccgaagcgcc
    15481 cttttcaatt caaaactaat taacgtgtaa ttagcccaga actttagcaa caacgcccgc
    15541 gccaacggta cggccgcctt cacggattgc gaaacgcaga ccgtcgtcca tcgcgatcgg
    15601 gtggatcagg gtaacaacca ttttgatgtt gtcgcccggc attaccatct ctacgccttc
    15661 cggcagttcg atggtaccag tcacgtcagt agtacggaag tagaactgcg gacggtagcc
    15721 tttgaagaac ggagtatgac ggccgccttc atctttggac agaatgtaca cttcagattc
    15781 gaacttggtg tgcggcttga tggtgcccgg cttagccagt acctgaccac gttcgatttc
    15841 ttcacgtttg ataccacgca gcagaacacc tacgttctca ccagcacggc cttcgtccag
    15901 cagtttgcgg aacatttcaa cgccagtaca ggtagacttc tgagtctctt tgataccaac
    15961 gatttcaact tcttcaccaa ctttgatgat accgcgttct acacgaccgg taacaacggt
    16021 accacgaccg gagatggaga atacgtcttc gatcggcagc aggaacggct tgtcaatcgc
    16081 acgctctggt tccggaatat aagaatccag gaagccagcc agttccagga ttttcgcttc
    16141 ccactctgcg tcgccttcca gcgctttcag agcagaacca cgaacgatcg gagtgtcgtc
    16201 gcccgggaag tcgtactgag acagaagttc acgaacttcc atttcaacca gttccagcag
    16261 ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg atgatgtacg gaacgcctac
    16321 ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc gggccgtcag tcgcagcaac
    16381 taccaggatc gcgccgtcca tctgagcagc accggtgatc atgtttttaa catagtcggc
    16441 gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg gtgtcatatt caacgtgaga
    16501 agtgttgatg gtgataccac gagctttttc ttccggcgcg ttatcgatct ggtcgaatgc
    16561 acgagcagca ccgccgtagg ttttagccag tacggtggtg attgcagcgg tcagagtagt
    16621 tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg tgcggttttg tacgttcaaa
    16681 tttttcttta gacacggcta tattccttac tatagtgctc tccccttcag gagagagcac
    16741 gggactttgg tattaaccct taggcttatt taccacgggc ttcaattacg gcctgagcaa
    16801 cgttactcgg cgcttcatca tacttcagga attccatagt gtatgatgca cgacctttgg
    16861 tcagagaacg cagctgagtt gcgtatccga acatttcaga cagcggtact tcagcgtgga
    16921 tcttaacgcc agtaacttca gattcctgac ctttgagcat accacgacga cggctcaagt
    16981 caccgataac gtcaccggtg ttctcttccg gagtttctac ttcaaccttc atgatcggct
    17041 caagcagaac tggtttcgct ttcttaaagc cttctttaaa ggcgatagaa gcagccagtt
    17101 taaacgccag ttcagaggag tcaacgtcat ggtaagaacc gaagtgcaga cgaataccca
    17161 tgtctactac cgggtagcct gccagcggac ctgctttcag ctgttcctgg atacctttat
    17221 caacggccgg gatgtattcg ccagggatta caccaccttt aatgtcgttg atgaactcgt
    17281 agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc gataacaaca tgaccatact
    17341 gaccacgacc accagactgt ttcgcgtgtt taccttcaac atcggtaact ttctggcgga
    17401 tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc ttcaacgttg aattcacgct
    17461 tcatacggtc aacgatgatg tcgaggtgca gttcgcccat acccgcgatg atggtctggt
    17521 tagattcttc gtcagtccat acacggaaag acgggtcttc tttagccaga cggcccagag
    17581 ccagacccat tttttcctgg tcagctttgg ttttcggttc aactgcgatg gagattaccg
    17641 gctcagggaa ttccatacgt tccagaatga tcggcgcatc cgggtcacac agggtgtcac
    17701 cagtggttac gtctttcaga ccgatagcag cagcgatgtc gcccgcgcga acttctttga
    17761 tctcttcacg tttgttagcg tgcatctgaa cgatacgacc gaaacgctca cgtgcagctt
    17821 tcacggagtt cagtacggta tcaccagagt taaccacacc ggagtaaaca cggaagaagg
    17881 tcaggttacc aacaaacggg tcggtagcga ttttgaacgc cagtgcagag aacggctcgt
    17941 catcacttgc gtgacgttca gccggagtgt ctttaccgtc gtccaggata ccgttgatcg
    18001 caggtacgtc aaccggggat ggcaggtaat caattaccgc atccagcatc gcctgaacac
    18061 ctttgttctt gaacgcagaa ccacaggtta ccaggatgat ttcgttgttc agaacgcgct
    18121 gacgcagagc acctttgatt tctgcttcag tcagttcttc accacccagg tatttttcca
    18181 tcagctcttc agaagcttca gctgcggatt cgatcaggtt ctggtgccat tcgttagcca
    18241 gttcaaccat gtctgccggg atatcttcgt attcgaaggt tacgccctgg tcagcgtcgt
    18301 tccagttgat agctttcatt ttcaccaggt caacaacacc ggtgaaatgt tcttcagcac
    18361 caatcgccag ctgcagcgga accgggttcg cgcccagacg ggttttgatc tggttaacaa
    18421 ctttcaggaa gttcgcaccc acgcggtcca ttttgttaac gaacgcaatg cgcggaactt
    18481 tatatttgtt tgcctgacgc catacggttt cagactgcgg ctgaacacca ccaactgcgc
    18541 agtaaaccat taccgcacca tcgagaacac gcatggaacg ttctacttcg attgtgaagt
    18601 caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg ctcatactgc ttagccatac
    18661 cagaccagaa tgcagtagtc gcagcggaag tgatggtaat accacgttcc tgctcctgct
    18721 ccatccagtc catggttgca gcgccgtcat gaacttcacc gattttatgg tttacaccgg
    18781 tgtagaacag aatacgttcg gtagtagtgg ttttaccggc gtcgatgtgc gcactgatac
    18841 cgatgttacg gtagcgtgcg atgggtgttg tacgagccat ttgtttcctc gtttatcttt
    18901 taggcgttca atttaagtag cccaaagcgg gctgcttact ggaagcgccc gcctggtgac
    18961 taaaactccg aagggattac caacggtagt gtgcgaacgc cttgttggct tcggccatac
    19021 ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt ttctgcagca tcagaaagtt
    19081 cgttcgccag gcgcagagcc atggatttat caccgcgttt acgagcagct tcaacgatcc
    19141 aacgcattgc cagagcatta cgacgaaccg gacggacttc aactggtacc tgataagtag
    19201 aaccaccaac gcggcgagac ttaacttcta cagtcgggcg cacgttttcg agagctactt
    19261 cgaatgcttc cagttcagat ttaccagagc gctgagccag ggtctccagc gcgctgtata
    19321 cgatagattc agcagtagat tttttaccat ctaccatcag gatatttaca aatttagcca
    19381 gcagttctga tccgaacttc ggatccggca gaattttacg ctgaccaatg acgcgacgac
    19441 gtggcatgga aatactccgt tgttaattca ggattgtcca aaactctacg agtttagttt
    19501 gacatttaag ttaaaacgtt tggccttact taacggagaa ccattaagcc ttaggacgct
    19561 tcacgccata cttggaacga gcctgcttac ggtctttaac gccggagcag tcaagcgcac
    19621 cacgtacggt gtggtaacga acacccggga ggtctttaac acgaccgcca cggatcagga
    19681 tcacggagtg ctcctgcagg ttgtgacctt caccaccgat gtaggaagtc acttcgaaac
    19741 cgttagtcag acgaacacgg catactttac gcagcgcgga gttcggtttt ttaggagtgg
    19801 tagtatatac acgagtacat acgccacgtt tttgcgggca tgcttccagc gcaggcacgt
    19861 tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag ctggttaact gttgccatta
    19921 aatagctcct ggttttagct tttgcttcgt aaacacgtaa taaaacgtcc tcacacaata
    19981 tgaggacgcc gaattttagg gcgatgccga aaaggtgtca agaaatatac aacgatcccg
    20041 ccatcaccag gccatctggc tggagtgctt aaccgtaagt ctgacgaaat cagtatagtc
    20101 aatgggaatg atgtcgttca aaatttgaac agtcaaaccg cgggcaataa ggtcttcgtt
    20161 cagggcatag accttaatgg gggcattacg cagactttca aggtagcggt taccgtcaac
    20221 tgcggcagtt acgccatctt gcaataatag cagttcgtct ccttcactga gcagacgcag
    20281 cagcgcagca aaatccgtca gccagggtga acgatgtaac gtgtgcagca taaacgcctc
    20341 aaaacctcaa aataacatcg tagttggcga gttcgcggcg taaggcatct gcttcgagcg
    20401 gcgtggcttc gacaacaaag ggtgtctgcg gatctaaccc gcgttcgcgc agtgaagccg
    20461 cacaaaccca gcactgttca atgtcgtaca gactcaacaa tttaaaagtg gcaatgtaat
    20521 cacgcgccag cactgcatcg ggcttttgtc ctgacagcag ctgaaaaacg ccatcagcaa
    20581 taaagaagac ggccagattg tcagttaatg cggaagttgc cagtaaagca tctaaacctt
    20641 cccgaccagc agctgtacca tgaggtgcag tagaaaaaac aaacgcaatt cgtttcatca
    20701 gaactgtacc accctgtcac aggtcagcga ggcttccgcc agcgcgccaa gtccgcttaa
    20761 ggtaaatccc ggctgaaggt ttgacgaagc cagccccagc cttcctgctt cagtttcatc
    20821 gacgataccg cggcgtaacg ctgccgctac gcagatattc agcgccacac catgttgcgc
    20881 attcaactgt tgccagccgc gcacaaggtc aaattcgtcg cttgccggtg aggtcagttg
    20941 gttagcgtta tagacccctt cccgatagaa aaagacgctg cttaacttat ggccttctgc
    21001 tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt tgcgtaccgt atgctggccc
    21061 ggtcaccacg atggcaaaac gcattactta tcttgcccct gaaaatcacc gctcttgaac
    21121 tggcggatgt agagatagac agtgtgtttg gagatgttca ggcggtcagc aacctggttg
    21181 atcgcatctt taatatcgaa gatccctttc tcgtagagat tcagcacgat ctgacgattt
    21241 ttggcgttat tagaaacatt gcgatcggca ttcacttctt cgatggtgaa ctccagcgtt
    21301 tgggtaacca gatcttcaac agaagaggca aagttgacgc ttgaaccgac atccggggtt
    21361 tctggcggca caaaagtgct cataatctgc gagaagggaa catcaagatt catattgatg
    21421 cacagcagac caattacacg ctgttcgcgg ttacgaatcg cgatagtcag ggacttcatt
    21481 aatacgccgc ttttggcgcg agtaaagtag catttagaaa cgctgctatc cgctcccgtc
    21541 atatcgtgca gcatacgtag cgccaggtca gtaattggcg aaccaatctt ccggcctgta
    21601 tgttcaccgt tagcaatgcg aatggctgaa cattttagat cctgcaaaga gtgcaaaacg
    21661 atttcacagt gggagccaat aagcatcgct aacccgtcca ccaccgcttc gtaggatttc
    21721 agaatatcaa aatcggtctg gtcgaaagga cgttgatcca gtaaatccaa ctcactggtt
    21781 tcgttggtta aaagcgacct ggacatgaaa aaaaacactc cttttcagga gcctgtcgtt
    21841 aacttttcag ggcaggctca ttaatgatgc gggtaactaa attaatacag cggaggttcc
    21901 gctttccagc actaattata tccggcctgt aataaaaaaa ccgccgcctg gtcaggcggc
    21961 ggttcttaat gcttattttt tagcagaatc tgcggctttc gcatcagctt ccggctttgc
    22021 atcagccttc ggcgctggtt tcacatccag cagctctacg tcaaacacta gggtagaatt
    22081 cggtgggatc cccggaacac ccgctttgcc gtaagccagt tctggtggaa taaccagttt
    22141 gatcttaccg cctttcttga tgttcttcag accttctgtc caacccggga taacaccgtc
    22201 cagacggaaa gaaagcggtt caccacgggt gtaagagttg tcgaactctt taccgtcgat
    22261 cagcgtacct ttgtagttca ctacaacagt atcgctgtct ttcggtgctt cgcctttacc
    22321 ggcttctact acctgataaa ccagaccagt tgaagaggtt ttcacacctt tctctttggc
    22381 aaatttctcg cggtactctt taccttttgc ttcgttatca gccgcgtctt tttccatctt
    22441 cgcctgagca gaagacttca cgcgagcttc gaatgcttgc agagtctgtt cgatctcttg
    22501 gtcggagagt ttgctcttat cagcaaatgc atcctgaaca ccagcgatca gctgatcttt
    22561 atccagtttg atgcccagtt tttcttgttc tttaagagag ttttccatgt aacgacccag
    22621 cgaagcaccc agtgcataag ctgatttctg atcgtcattt ttgaacgatg ctttgctgtc
    22681 tgcagttgta gcaggttttg cagcttcagc agcaaaagtg attggtgcat gcagggcaac
    22741 ggccattgtg gtcgccagta gcgttacttt aaacagtgat ttcatccata tctccaggat
    22801 cggggcatct caccccaggg ttaactatta tcagaagggt actataaagc gttgtcgaac
    22861 aaatctacat acagacacgc cctattatca tctattttca gactcttttt gtttaaatta
    22921 gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa agttaagtag aatccgcggc
    22981 gaccattcga caaaagaggt gaatcatgca ggatttatca ttggaagcac gcctggcaga
    23041 gctggagagc cgactggctt ttcaggaaat caccattgaa gaactgaacg tcacggtgac
    23101 cgctcatgaa atggagatgg cgaaactgcg cgatcatctg cgtctgctga ccgagaagtt
    23161 aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa gaaacgccac cgccacatta
    23221 ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc accttttcgg tattagtggc
    23281 aaccacaacc gccgttgcct ttaccgccac agcagccttc gccaccgtgt tcatgaccgt
    23341 gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg atcgtggtga tgatcgtgcg
    23401 cgccgtgaac gtggccatga gccagttctt cttcggtcgc ttcgcgaatc gccacaactt
    23461 caacgttgaa tttcaggttc tgaccggcca gcatgtggtt accatcaacc actacgtgat
    23521 cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg gtcagtttca gccaggaaac
    23581 gcatacctac ctgcagttca tcaacgccca taaatacgtc tttaggaaca cgttgcacta
    23641 ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac agcgacatca aatttgtcgc
    23701 caacttcatg accttccagc gccgtttcca ggccagagat cagggaaccg tgaccatgca
    23761 ggtagtccag cggcgcactc accggagact catcaaccaa cacaccgtct tctgtacgta
    23821 cctgataggc caggctgacc accaggtctt ttgctacttt catgatatct cctgagcatg
    23881 ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc gtgtactcac tagcttaaaa
    23941 aaactcggcg catatcgcta gtccggatga aaaatcccga tcacttgctc atctttgtga
    24001 acgtgatcgc gggcttcttt gtctgcttct cgcatctgat gtccgcactt aacacattca
    24061 acaatatcaa tattattttc gcgccacatc gccattgaat cctgcgcctg acaggccggg
    24121 cattttgcgc ccgctataaa acgttttcgg attgccatcc ttaccctcta ctcaaattca
    24181 tcccagccgt ccagctggcg tcgttcttgt tgcatttcac gctggaaaat ctcttccagt
    24241 tcgcgtcggg cttccctggc gcgagaaatt tgtacggtat cagcatgcat cgggattaac
    24301 tcccgcagca ttcgcatatc caggcggcga aaatgcagtt gcgcccgctg tgcctgatgc
    24361 ggatgcatgc caagcgtgac cagcgtcttg cgccccagct ctaacgcact ggagaatgtt
    24421 tcacgggaaa actgcgtcac ccctgcctgt aataactcat gcgcttccac acgtccgcgc
    24481 gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc atatttccac cagcttcatg
    24541 gtgtcttccg gctcgttaca ggtaatgacg atagactcag cggcctctgc acccgcagaa
    24601 cgtaaaagat cgacctgcgt ggcgtcgccg taataaactt tgtagccata tttacgcatc
    24661 aggttaacgg cgctgatatc ccgctccagc acggtaatgc gcattttatt tgccatcagc
    24721 aaacgaccaa tcacctgacc aaaacgcccg aagcccacga caatgacctg gggtttatca
    24781 tcgttgaccc acggtttttc gtcttcttct tccggtccgt taaactggcg ggatagccat
    24841 ttatccacca gcttcatcag caacggcgcc atctggtcgc cctggaataa acgttgtgaa
    24901 gaagcggtag aaaagaggac aaaggcaaac tcaccgccct gactcaacac gccagcaaac
    24961 tgcatccgct ctgagctacg cacgccatac aatcgcgcca gcagatacag cacgagaatt
    25021 ttcaccgcca ccagcacaac cacgcttatc actacccaca acagatgggt ataaagcacc
    25081 ccgaggttga gcgacatgcc gacagagata aagaacaaac cgagcagcaa gcctttgaag
    25141 ggatcgatag ccgtttccag ttcatggcga tattcacttt ccgccagcag cacgcccgca
    25201 ataaatgtac cgagcgccat cgacagcccc agcgcatcca taaacaatgc ggaacccaac
    25261 accagcagca gcgtcgcggc ggtgaacact tcccgcacgc cagaagctgc aataaagcgg
    25321 aataccggac gcagtaaata gcgcccacca atcagcatgc cgacaaactc cagcaccttc
    25381 atgccgatct tcatccagtc gaaatgttcg tctgccgacc ccgccaacaa cggcactaac
    25441 gccagtgctg ggatcactgc cagatcctga aacagcagaa ccgaaaaccc gagctggccg
    25501 gattcgctgc gattcatccc tttctcacgc atcaattgca acgccattgc agttgaagac
    25561 atcgcaaagc caatgccacc gaccaccgcc gcctgccagg cgaaatccgt cagcatcaat
    25621 aatcccgcca gcaacgccgc gcttaacagc acctgtgccg cgcctacgcc aaaaatcgaa
    25681 cgccgcagtt gccaaagttt ggagggattc aactcaaggc cgatgataaa catcaggaat
    25741 accacgccga gttccgaaaa gtggaggatc tcatcgacgt cgctaataaa ccccagcccc
    25801 cacgggccaa ttgcaatccc cgccagcaaa tatcccaaca cagcgccaat ccccagccgc
    25861 gaagagaaac agcactcctg cgagtaaaaa atcggaacct tccatcagcg gcctcctgga
    25921 gacagcggat ttgccaacca gtcaccgtag gctctggcgt ggctcgccag ctcctttgcg
    25981 ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg gcacatgccc
    26041 gccgccagtt caaaggggcg cagcacatcg ctcatcgggt agcgattcag cgcgtcataa
    26101 cggtaagcac tttccggctc gccggtggta atcacgttac gccagtactt tcccgccagt
    26161 tggtttcctc ccggcccgct ggcaaaacca cgacttaata cccggtccag ccactctttc
    26221 agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac ctcgtgctcg
    26281 cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg cgcgtaaagg
    26341 tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg gtttgccacc
    26401 gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg agacatcatc
    26461 cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg cgcccggtgc
    26521 ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct gaacatacgg
    26581 cgccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt cctgctggat
    26641 aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa aaacggctgt
    26701 ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg cggcagctac
    26761 acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccagc gttaccgcaa
    26821 gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga agcgcagctg
    26881 cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatag caagctggat
    26941 gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg cctcggtttc
    27001 agcaatgaac aactggagcg cccggtaagt gatttctccg gtggctggcg tatgcgtctt
    27061 aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga accgactaac
    27121 cacctcgatc tcgatgccgt tatctggctg gaaaaatggt tgaagagcta tcagggcacg
    27181 ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgttgataa aattattcat
    27241 atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga agtacagcgc
    27301 gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg cgtggcacat
    27361 ctgcaaagtt atatcgatcg tttccgtgcc aaagccacca aagcgaagca ggcccagagc
    27421 cgcattaaga tgctggaacg tatggagctg attgcccccg cacacgtcga caacccgttc
    27481 cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat ggaaaaggtc
    27541 agcgcaggct atggcgatcg cattattctc gactcgatta aattgaatct ggtccccggc
    27601 tcgcgcattg gtctgttagg ccgcaacggc gcaggtaaat cgacattaat caaactgtta
    27661 gccggtgaac ttgcgccagt cagcggtgaa attggtctgg cgaaagggat caagctcggc
    27721 tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcacctat tcaacatctg
    27781 gcacgtttag cgccgcagga gctggaacaa aaactgcgtg actacctcgg cggctttggt
    27841 ttcctgggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga aaaagcccgc
    27901 ctggtgctgg cattaattgt ctggcagcgt ccgaatctgc tgctgctcga cgaaccgact
    27961 aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga gtttgaaggc
    28021 gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga cgatctctac
    28081 ctggttcacg atcgtaaagt cgaaccgttc gacggcgatc tggaagatta tcaacagtgg
    28141 ttgagcgacg tacaaaagca ggaaaaccag accgacgaag cgccaaaaga gaacgcgaac
    28201 agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agctgcgtgc gcaaacccag
    28261 ccgctgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa cgcgcaactg
    28321 gcgcaggcgg aagagaaact cggcgacagc gaactgtatg accagagccg taaagcggag
    28381 ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga gtgcgaaatg
    28441 gcgtggctgg aagcccagga gcagcttgag cagatgctgc tggaaggcca aagcaactga
    28501 tggcgcagat aacgacgacc gatgccaatg aatttagcag cagtgctgaa ttcaccccta
    28561 tgcgcggctt tagcaattgt catctacaaa ctatgctgcc gcgtttgttt cgtcgtcagg
    28621 tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt gtcgatctcg
    28681 cgtggagtga agaccccgca caggcgaaac ataaaccgcg tttagtggtg tttcacgggc
    28741 tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg caaaagcgcg
    28801 gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac cgtatgcacc
    28861 gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg ctgcaacgcg
    28921 aatttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat atgctggcct
    28981 gtttgctggc aaaggaaggc aatgatctcc cggttgatgc ggcggtgatt gtctctgcgc
    29041 cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc gtttatcagc
    29101 gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc taccccggaa
    29161 cgctgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa tttgacgatc
    29221 taatcaccgc cagaattcac ggctacgccg acgctatcga ctattatcgt cagtgtagcg
    29281 ccatgccaat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc aaagacgatc
    29341 cgtttatgga tcatcaggtg atcccaaaac cggaaagtct cccctcgcag gtggagtatc
    29401 aactgactga acatggcggt catgttggct ttattggcgg tacattactt catccgcaaa
    29461 tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg aaatcatgtt
    29521 gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa gctttgtgtt
    29581 acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag tcgccgacgt
    29641 caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc acgaaacggt
    29701 caatattatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg gacgagataa
    29761 tagtgatagc taagtcatgg agttactatg tctgccaaac atccggtcat tgcggtaaca
    29821 ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat attcgcgcag
    29881 ttaaatctgc atgcagctga ggtggaaggc gacagttttc accgttacac ccgcccggaa
    29941 atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta cttcggcccc
    30001 gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca gagcggcaaa
    30061 gggaaatctc gcaaatatct gcatacctac gacgaagccg taccctggaa tcaggtaccg
    30121 ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt ttatgaaggt
    30181 ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga cttactggtc
    30241 ggcgtagtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga caccagcgag
    30301 cgcgggcact cacgagaagc agtgatggac tcggtagtgc gttcaatgga agactatatc
    30361 aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt tcccaccgtc
    30421 gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag ctttgtggtg
    30481 atccattttc gtaatctgga agggatcgat ttcccttggc tgctggcgat gttgcaaggc
    30541 tcattcattt cccacatcaa tacgttagtg gtacctggcg gtaaaatggg gctggcaatg
    30601 gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat cgagtaactt
    30661 tgctattatg ccggataaaa tactatccgg cttcacaacg ggatagttaa gtcacgcggc
    30721 aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg ccactgaaca
    30781 atatttctcg gcagagagat caaccgcacg cgcaaccgct gcgtctttca ggtcgcgacc
    30841 ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt cgcggcgttc
    30901 agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca ggatcgaaac
    30961 cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg gacttggtgc
    31021 tttatcgcct gagttgccgt ccattaaaat ctggtgacca gaggcggatt cgcccagaaa
    31081 agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac tgcgtcaatt
    31141 ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct gggtcatgct
    31201 gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag gatgctacag
    31261 taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca gtacagttga
    31321 tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc ggcgttatct
    31381 ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca aaccgcaaac
    31441 agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc catccaagag
    31501 cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta aaggctctgt
    31561 ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc tgaatcaggg
    31621 tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg catgggtacg
    31681 tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc aattgattca
    31741 ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc tgcaagtcac
    31801 ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg cacagactct
    31861 gctgaacctg gcaaaacaac cagatgctat gactcacccg gacggtatgc aaatcaaaat
    31921 tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg gacgcattct
    31981 gaagatgctg gaagatcaga acctgatctc cgcacacggt aaaaccatcg tcgtttacgg
    32041 cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt gtttcccccg
    32101 atgtggcgca gactgattta tcaccccgat atcaactatg cacttcgaca aacgctggtg
    32161 ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg tctgctcttc
    32221 tccctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa acgttttttc
    32281 aaacacttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac acagctgcta
    32341 ctggtaaaag atgttcccct gccctttttg ctgaccggat taacgctggt acttggcgtc
    32401 actgctgagc tggggccatt gcacgcaaaa ttgcttcctg catcgctgct cgccgccatt
    32461 tttaccctca gtctggcggg atacatgccg gtctgggaac cattgctcat ctatgcgttg
    32521 ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg cgaacaaccg
    32581 ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga agccaaatac
    32641 agcctgctta cccagcacac cgatcctgaa aaagcgctgc caccgctgct ggtgcgccag
    32701 caaaaagcgg tcgatttaat tactcagtgc tatcagcaaa tgcatatgct ttccgcgcaa
    32761 aacaataccg attacaagcg gatgctgcgt attttccagg aggcgctgga tttgcaggaa
    32821 catatttcgg tcagtttgca tcagccggaa gaggtgcaaa aactggtcga gcgtagccat
    32881 gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg cgtgctggct
    32941 gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca aattggcgca
    33001 ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg ctactggcat
    33061 ttcagccgca tcgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg tgacttactg
    33121 gccgataaac agcggcgaat gccgttgctt ccggcactga aaagttatct gtcactaaag
    33181 tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc cagcctgatg
    33241 ggcaccgtgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt gctggtgaca
    33301 caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg aaccgtggtc
    33361 gggttaatca ttgcgggcgt agcgctgcac tttaaaattc ccgaaagtta caccctgacg
    33421 ttgatgctga ttaccaccct cgccagctac ctgatattgc gcaaaaacta cggctgggca
    33481 acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct gaacggtgag
    33541 cagtacatcc ttccacgtct tatcgatacc attattggtt gtttgattgc tttcggtggt
    33601 accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc ccatgatgct
    33661 ttagaagcct atcaggaagc gattcgcttg attctgagcg aggacccgca accaacgcca
    33721 ctggcctggc agagaatgcg ggtaaatcag gcgcataaca ctctgtataa ctcattgaat
    33781 caggcgatgc aggaaccggc attcaacagc cattatctgg cagatatgaa actgtgggta
    33841 acgcacagcc agtttattgt tgagcatatt aatgccatga ccacgctggc gcgggaacac
    33901 cgggcattgc cacctgaact ggcacaagag tatttacagt cttgtgaaat cgccattcag
    33961 cgttgtcagc agcgactgga gtatgacgaa ccgggtagtt ctggcgatgc caatatcatg
    34021 gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca gcatttacag
    34081 cgggttattg gccatctgaa caccatgcac accatttcgt cgatggcatg gcgtcagcga
    34141 ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaaggcgta atacaggcta
    34201 aaccatcgga taaggcattc acgccgcatc cgacatattt tgcctgatac gtcttattta
    34261 cgccccaacc accttcgcca ccgcgtgggc gaaacgttgc atcccttcat cgatatccgc
    34321 atcttccacc accagcgacg gtgctaaacg catcacatcc ggtccggcat tcagcaccat
    34381 tacgccaacc tctgcacccg catacaggaa atcacgcgcc cgacctttgt agtgtggttt
    34441 cagctccgcg ccaattaaca gccccatacc gcgaatatcg ctaaacacat cgtactgctg
    34501 atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc cttccagcac
    34561 ttcaggggta ttgatgatat caaacgccgc gcccgctact gcacaggcca gaggattacc
    34621 gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg ccgtggtcag
    34681 catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa tatccggcgt
    34741 cacaccgtag tacctgtaag caaacaaatc gccagtccgc cccatcccgc actgcacttc
    34801 atcaaacacc aataatgcct gatgttgatc gcacagctcg cgcaagccct gcaaaaactc
    34861 tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca ccgcacaggt
    34921 gtgatcatcc atcaccgctt tcactgcatg gagatcgtta aagggaacgt ggatgatgtc
    34981 tgccggtttc ggcccaaagc cgtcggaata ttttggctgc ccacccaccg aaacggtaaa
    35041 cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga acgggctatg
    35101 acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt ccgtgccgga
    35161 gttcataaac accacgcgtt cggcaaacgt cgcctcaatc agtttgcgcc caagacgcag
    35221 cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct gggtttttaa
    35281 cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc cacccgcgaa
    35341 atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt ttaccggaat
    35401 aaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc gtgtaattgc
    35461 tgtttgttca attgccatct catgatcacc ctgttacgca taaataaatg tgaaattata
    35521 atcacaaaat atgcataaaa aatcactgaa tggcaatcgg aaatcagaga tgcaggaaat
    35581 tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac tgcacacctt
    35641 ccagatccca ctggcgatgg cgaatcccca taatctctcg ggtttcgctc caggccgtca
    35701 cgtcaaagca cgctggtaat gagtcaggtt ccaccaccag cgaatggtag cgtgtcacgg
    35761 taagtggatt tgccagcccc cgaaatacgc cctcaccgct atgtgtaatc ggcgaggttt
    35821 tgccgtgcat gacctttgcg gcgcgcacaa ctttgccgcc aaatgcctgc gccattgcct
    35881 gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga ataacgtcaa
    35941 gggagatccc ggcttcatct ggcgtacagg ggccaggtga gatgacaatt ttttgtggtt
    36001 taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc acatccgccc
    36061 ccagttcaca aaagtactgg tagaggttcc aggtaaaaga atcgtagtta tctataagca
    36121 ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc cccggcttcg
    36181 cttaccactt tgctaaatat catctgcagt gcggtgagat cccccattgc cccactctga
    36241 tttgcctgat tccaggcctc tttttcgata ccctgccagc tcagttgata tccggcatga
    36301 atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg gaagggatgc
    36361 agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc tttctccagg
    36421 ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc tttttcgata
    36481 taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg cagttgccct
    36541 gcccagtcga aaatatcctg gtacagctgg cgatggatag tgcgcaaatg cggtaaaccg
    36601 cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc gtaagcggcc
    36661 tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc aaggccagga
    36721 taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc tcagctcttc
    36781 aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga cgccttcaag
    36841 acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg acttttgctt
    36901 atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac aagtataagc
    36961 ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg cgcgagaaat
    37021 cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac attctggtac
    37081 ggaccaacat catgagtcgg cacctgggaa atcttatcgg caacgtccat gcctttcacc
    37141 actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa ggcgttatcg
    37201 gcaacgttga taaagaactg gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg
    37261 atggtgccac gcgtgttgcg caggccgtta tcggcttcat ttttgattgg cgggtttggt
    37321 tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg
    37381 cggtgaaagg tagtgttgtt ataaaaaccg ctgttcacat aatcgacaaa gttttgcaca
    37441 gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga ggttgtcaac
    37501 aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga aagagcagaa
    37561 agagcgaaaa cagcagccat cgccgccagg gtcgatttga acatgagatt tccttaacaa
    37621 acagagcaga aaaaaagcaa gtgaagagat tctaaatagc cttcaagaac aaggccatcc
    37681 ctttacctaa ttttacgtat ctgaaatata tgtaacctga cgatcacttt ttattccgtg
    37741 atcaaaatca cctcttaaaa tgcaatttag caaccgattg caataaaaca tttaaacaga
    37801 tcataaaatc acctaaaatc gcccgctcgc ggcatatccc gctatgccat tttatttttg
    37861 aacgcaggcc aatcatgact aacagcaatc gcatcaagct cacatggatt agctttctct
    37921 cctacgcact gactggtgcg ttggttattg tcaccgggat ggtgatggga aatatcgccg
    37981 attatttcaa tctgcctgtt tccagtatga gtaacacctt caccttcctc aacgccggca
    38041 ttttaatctc tatcttcctc aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt
    38101 tacgttttgg ctttctcctg atggtgctgg cggttgccgg tttgatgttc agccacagcc
    38161 tggcgctgtt ctcgacggcg atgttcattc tcggggtggt cagcggcatt actatgtcga
    38221 ttggtacatt cctgataaca caaatgtatg aagggcgtca gcgcggttcc cgcctgttat
    38281 ttactgactc cttcttcagt atggctggga tgattttccc aatgatcgcc gcgtttctac
    38341 tggcgcgcag cattgagtgg tactgggttt atgcctgcat cgggctggtg tatgtcgcta
    38401 tttttattct gaccttcggc tgtgagttcc cggcactggg caaacgtgca ccaaaaacgg
    38461 atgctccggt agagaaagaa aagtggggga tcggcgtact gtttctctct gttgcggcgc
    38521 tgtgctacat cctcggtcag ttaggtttta tctcctgggt gcctgagtat gccaaaggcc
    38581 tgggcatgag cctgaacgac gcgggcacgc tggtgagtaa cttctggatg tcatacatgg
    38641 tcggcatgtg ggcgttcagc tttattcttc gcttctttga tttgcaacgc attctgaccg
    38701 tactggctgg tctggctgcg attctgatgt acgtctttaa caccggaaca ccagtacata
    38761 tggcgtggtc aattctcgct ctgggcttct tctccagcgc gatctatacc accatcatca
    38821 ccctgggttc acagcagacc aaagtaccgt cgccaaaact ggttaacttt gtcctgacct
    38881 gcggaaccat cggtactatg ttgacctttg tggttaccgg cccgattgtt gaacatagcg
    38941 gtccgcaggc ggcactgctt acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct
    39001 tcctgttagg tttcgtcagc cgtcaccgtc agcataacac cctgacctct cattaattgc
    39061 taatgccgga cggcactatc gtcgtccggc cttttcctct cttcccccgc tacgtgcatc
    39121 tatttctata aacccgttca ttttgtccat tttttgcaca aacatgaaat gtcagacaat
    39181 tccgtgactt aagaaaattt atacaaatca gcaatatacc ccttaaggag tatataaagg
    39241 tgaatttgat ttacatcaat aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa
    39301 aagaaatcga ggcaaaaatg agcaaagtca gactcgcaat tatcggtaac ggtatggtcg
    39361 gccatcgctt tatcgaagat cttcttgata aatctgatgc ggccaacttt gatattaccg
    39421 ttttctgtga agaaccgcgc atcgcttatg accgcgtaca cctctcgtct tacttctctc
    39481 accacaccgc cgaagagctg tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca
    39541 aagttctggt cggcgaacgc gctatcacca tcaaccgtca ggagaaggtg attcactcca
    39601 gcgccggacg taccgttttt tatgacaagc tgatcatggc aaccggttcc tacccgtgga
    39661 tcccaccaat caaaggttct gatactcagg actgctttgt ctatcgcacc attgaagacc
    39721 tcaacgccat tgaatcctgc gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc
    39781 tgttaggtct ggaagccgct ggcgcgctga aaaacttagg tattgaaacc cacgttatcg
    39841 aatttgcccc tatgctgatg gcggaacagc ttgatcagat gggtggcgag cagctgcgtc
    39901 gcaaaatcga aagtatgggc gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc
    39961 aggaaggtgt cgaagcgcgt aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg
    40021 actttatcgt cttctctacc ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc
    40081 tggacgttgc tccgcgtggg gggattgtca ttaatgattc ctgccagact tccgatccgg
    40141 atatctacgc catcggtgaa tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac
    40201 caggctacaa aatggcgcag gtcgccgttg accatattct gggtagcgaa aacgcctttg
    40261 aaggtgctga ccttagcgcc aagctgaaac tgctgggcgt agacgtaggt ggtattggtg
    40321 atgcgcacgg tcgcacgcct ggcgcacgta gctatgttta cctcgacgaa agtaaagaga
    40381 tctacaaacg cctgattgtc agcgaagaca acaaaaccct gctcggtgcg gtactggtgg
    40441 gcgataccag cgactacggc aacctgctgc aactggtgct gaacgctatc gagctgccgg
    40501 aaaacccgga ttctctgatt ctgccggcgc actcgggtag cggtaagccg tctatcggtg
    40561 ttgataaact gccggacagc gcgcaaatct gctcctgctt cgacgtcact aaaggtgatc
    40621 tgattgctgc catcaacaaa ggctgccaca cagttgcggc gctgaaagct gaaaccaaag
    40681 cgggtactgg ctgcggtggc tgtatcccgc tggtcactca ggtactgaac gcggaactgg
    40741 cgaaacaggg catcgaagtt aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg
    40801 aactgttcca tttgatccgc gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac
    40861 acggcaaagg ctacggttgt gaagtttgta aaccaaccgt cggttcgctg ctggcctcct
    40921 gctggaacga atacattctg aagccggaac atactccgct gcaggattct aacgacaact
    40981 tcctcgctaa catccagaaa gacggcacct actcggtgat cccgcgttct ccgggcggtg
    41041 aaatcacccc ggaagggctg atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca
    41101 ccaaaatcac cggctcccag cgcctggcga tgtttggcgc acagaaagac gatctgccgg
    41161 aaatctggcg tcagctgatt gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac
    41221 tgcgtatggc gaaaacctgc gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg
    41281 tcggcctcgg cgtggaactg gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga
    41341 agttcggtgt ctccggctgt acccgtgaat gttcagaagc tcagggtaaa gacgtgggta
    41401 ttatcgccac tgaaaaaggc tggaacctgt atgtttgcgg taacggcggc atgaaaccgc
    41461 gtcatgcgga tctgctggca gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc
    41521 gcttcatgat gttctacatc cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag
    41581 aaaacctcga aggcggcatc gattacctga aagcggtgat cattgacgac aaactggggc
    41641 tgaacgctca tctggaagaa gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga
    41701 ctgaaacggt caatacaccg tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca
    41761 agcgtgaccc gaacgtgcag atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt
    41821 atgaacgtat cccggtaact ctggtggagg acaacgcatg agccagtgga aagacatctg
    41881 caaaatcgat gacatcctgc ctgaaaccgg cgtctgcgca ctgttaggtg acaagcaggt
    41941 agcgattttc cgcccgtatc acagcgacca ggtgtttgcg atcagcaaca ttgatccgtt
    42001 cttcgagtcc agcgtgctat cacgcgggct gattgcggaa caccagggcg agctgtgggt
    42061 cgccagcccg ctgaaaaaac agcgttttcg cttaagtgat ggcttgtgca tggaagacga
    42121 acagttttcc gtcaaacatt acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg
    42181 ttaatgtttt aacgggaggc gcaatgcctc ccctttttgc atggtcctgt aatgaccttc
    42241 ggtatattgc aggacatttt ttaaactttt tgttttattt tttgttttta ttttttaaag
    42301 gataatcaaa tgtttacaga cactattaat aagtgtgcgg ctaatgctgc gcgcattgca
    42361 cgcctgtcgg caaataaccc gctcggcttt tgggtcagct ccgccatggc gggcgcatat
    42421 gtgggtcttg ggatcatcct gattttcacg ctcggtaatt tgctcgaccc gtccgtacgt
    42481 ccgctggtga tgggcgcgac ctttggtatc gccttaacgc tggtgattat cgccggttct
    42541 gaactgttca ccggacacac catgttcctc acctttgggg taaaagcggg cagcatcagc
    42601 cacgggcaaa tgtgggcaat cctgccgcaa acctggctgg gtaacctggt cggttccgtc
    42661 ttcgttgcca tgctctatag ctggggcggc ggtagcctgc tgccggtaga taccagcatc
    42721 gttcactccg tcgcgctggc taaaaccact gcaccggcaa tggtactgtt cttcaaaggt
    42781 gcattgtgta actggctggt ttgcctagca atctggatgg cgctgcgcac tgaaggggcg
    42841 gcgaaattta tcgctatctg gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac
    42901 tctatcgcta acatgacgct gttcgcgctc tcctggttcg gcaaccacag cgaagcctac
    42961 acgctggcgg gtattggtca taacctgctg tgggtgacgc tgggtaatac tttatcaggt
    43021 gccgtattca tgggattggg ttattggtat gctacgccga aagcgaatcg tccggttgcg
    43081 gacaaattta atcaaactga aacagctgcc ggttaattac taaggggttt ttacgtggat
    43141 catttgccta tattttgcca attacgcgat cgcgactgtc tgattgtcgg cggtggtgat
    43201 gtcgcggaac gcaaagcaag gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca
    43261 ttagcgttta ttccacagtt caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa
    43321 gggccatttg atgaaagcct tctcgacacc tgctggctgg cgattgcagc gacggatgat
    43381 gacacgctta accagcgcgt cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta
    43441 gttgatgcgc cgaaagccgc cagctttatt atgccgtcga ttattgaccg ctcaccgcta
    43501 atggtagcgg tctcctctgg cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa
    43561 ctcgaatcac tgctgccgtt gcatctgggc caggtggcga aatatgccgg gcaattacgc
    43621 gggcgagtga aacaacagtt cgccacgatg agtgagcgtc gccgtttctg ggagaaattg
    43681 ttcgttaacg accgcctggc gcagtcgctg gcaaacaacg atcagaaagc cattactgaa
    43741 acgaccgaac agttaatcaa cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt
    43801 gcaggtccgg gcgatgccgg gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca
    43861 gatgtggtgg tctacgaccg tctggtttct gacgatatta tgaatctggt acgccgcgat
    43921 gctgatcgcg ttttcgtcgg caaacgcgcg ggataccact gtgtaccgca ggaagagatt
    43981 aaccagatcc tgctgcggga agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc
    44041 gatccgttta tttttggccg tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt
    44101 ccgttctcgg tggttccggg tattaccgca gcttctggtt gctctgccta ttcgggtatt
    44161 ccactcacgc atcgcgatta tgcccagagc gtacgcttaa ttaccggaca cttaaaaacc
    44221 ggtggcgagc tggactggga aaacctggcg gcagaaaaac agacgctggt gttctatatg
    44281 gggttgaatc aggccgcgac tattcagcaa aagctgattg aacacggtat gcctggcgaa
    44341 atgccggtgg caattgtcga aaacggaacg gcagtcacgc agcgcgtgat tgacggtacg
    44401 ctcacgcaac tcggtgaact tgctcagcaa atgaacagtc catcgctaat tattattggt
    44461 cgggttgttg gcctgcgcga taaactgaac tggttctcca atcattaatt taacccgggc
    44521 cagagaattc tggccttctt aatgactctt tttatatatt cagcaaataa aacataaccc
    44581 cttaataaat attatctgct aatgggttat attcaccgtt acagcgactt acaaaattaa
    44641 gtcattcgct cactgacgcc aattaaatat tcctgtatcg ttcttaacaa gcagtctgct
    44701 tatattcata taatcaatga atattaatta atatataata catagggaat gtaaatgaac
    44761 aaatttatta aagttgcact ggtaggtgca gtactggcta cgttaactgc atgtactggt
    44821 catattgaaa accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct
    44881 atttctaaaa tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcgg
    44941 ctaacaccgg tttaattgcc cgacgccctc tggattttcc gagggcgtat ttgtttctat
    45001 gtccttcttg cccccgcccg taaaaatatt ttcactttaa attcaatttt ataactacat
    45061 ccattagcca cagcgcgatc ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca
    45121 taatatgttg ttatattcat catgcattgt catgttacct tttaaatgac tgcaaactct
    45181 cccctacaac gtattggaca agaaaaaggt atcgctatgg gaagccaaga actccaacgc
    45241 aagctcggat tttgggccgt tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt
    45301 tttgtatctg tgggtgaagt ggcaaaagca gcgggcacgc cgtggcttac ggtgctcgcg
    45361 tttgtcattg gcgggttaat tgtgatcccg caaatgtgcg tctatgcgga actatccacc
    45421 gcttatccgg aaaatggcgc agattatgtt tatctgaaaa atgccggaag ccgaccgctg
    45481 gctttcctct ccggctgggc cagcttctgg gccaacgatg cgccgtcatt gtcgattatg
    45541 gcgctggcga ttgtcagcaa tcttggcttt ttaacgccta tcgatccgtt gctcggtaaa
    45601 tttatcgccg ccggattaat tatcgccttt atgttgctac acctgcgctc cgttgaaggc
    45661 ggcgcagcgt ttcagacgct aattaccatc gccaaaatta tcccgttcac tatcgtcatt
    45721 ggccttggga tcttctggtt taaagcggag aattttgccg cccttaccac cactgcgatt
    45781 ggcgcaacgg gcagctttat ggcgctgctg gcggggatct ctgccaccag ttggtcgtat
    45841 accggcatgg cctctatctg ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg
    45901 ccacgagcgc tgattggttc ctgtctgctg gttctggtgc tctacaccct gctggcgctg
    45961 gtgatttccg gcctgatgcc cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac
    46021 gccctgacct agatccccgc actcggcagc accgctgggc tctttgttgc catcacggcg
    46081 atgatcgtca ttcttggttc gctttccagc tgcgtgatgt accagccgcg gctggaatac
    46141 gcgatggcga aagacaacct gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac
    46201 acgccggatg tctccatcat cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc
    46261 gatctcacca gcctgctggg ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc
    46321 ttcggctcca tcatctggtg tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg
    46381 gctttcgggc tgatgaccac cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc
    46441 tttgtctggg caccgattcc cggccttatc tgcgccgtca tcgttattgc tactggtctg
    46501 cctgcttacg ccttctgggc gaagcgtagc cgccagctca acgctttgtc gtaatgttat
    46561 ctgaagaaaa taaaatgttg gatattgata aaagcaccgt ggactttctg gtcaccgaaa
    46621 atatggttca ggaagtggaa aaagttctca gccatgacgt tccgctggtg cacgccatcg
    46681 tggaagagat ggtgaagcgc gacattgatc gtatttattt cgttgcctgc ggctcgccac
    46741 tcaacgcggc gcaaacggcg aaacatctgg cggatcgctt ttccgatctt caggtctacg
    46801 ccatttccgg ctgggagttc tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg
    46861 tgattggcgt ttctgactac ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc
    46921 gggcctgcgg cgcactcact gcggcgttca ccaaacgcgc ggacagcccg attacctcgg
    46981 cggcggaatt tagcattgat tatcaggccg actgtatctg ggaaattcac ctgctgctct
    47041 gctacagcgt ggtgctggag atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga
    47101 tcaaaaacga tctcaagcag ttgccgaatg cgctcggtca tctggtacgc acctgggaag
    47161 aaaaaggccg ccagcttggt gaactggcca gccagtggcc gatgatttat accgttgctg
    47221 cgggtccgct gcgtccgctg ggttacaaag aaggcattgt aacgctgatg gaatttacct
    47281 ggacgcacgg ctgcgtgatt gagagcggag agttccgcca tggcccgctg gagattgtcg
    47341 aaccgggcgt tccgttcctg ttcctgctcg gcaatgatga aagtcgccac accaccgaac
    47401 gcgccattaa ctttgttaaa cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa
    47461 tttcgcaagg gctgcacccg tggctggcac cgttcctgat gttcgtgcca atggagtggc
    47521 tctgctacta cctgtctatt tacaaagatc acaacccgga tgaacgccgc tattacggtg
    47581 gtctggtgga atattaatcc ctctcccggc ccggcaacgg gccggattta cgcaaggagt
    47641 tacccgatga aaacaggtat gtttacctgc ggccaccagc ggctgcctat tgaacacgca
    47701 tttcgtgatg caagcgagct gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac
    47761 gcgttcgcgc cggacttaaa agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg
    47821 tatcagatgc cgattatcgg ttacacgcca gaaaccaacg gctatccgta taacatgatg
    47881 ctgggcgatg aacatatgcg tcgtgaaagc ctggacatga tcaagctggc gatagatatg
    47941 gcaaaagaga tgaacgcggg ttatacgctg atttctgcgg cccacgcggg ctatctcacg
    48001 ccgcctaacg ttatctgggg gcggctggca gagaacttaa gcgaactgtg tgagtacgcg
    48061 gaaaacatcg gcatggatct gatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg
    48121 tgtaatgcta atgatgtgct tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc
    48181 atggtcgaca tttgcgcgcc gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa
    48241 ctgggcgata aattacgtca tctgcatatt gtcgacagcg acggggccag cgacacgcat
    48301 tacattcctg gagaaggcaa aatgccgctg cgggaactga tgcgcgatat tattgatcgg
    48361 ggctatgagg gttactgtac ggtggagctg gtgacgatgt atatgaacga gcccagactc
    48421 tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc cggaggatga gagatgaaaa
    48481 ccctggcgac aatcggcgat aactgcgtcg atatctaccc gcaactgaat aaagcgtttt
    48541 ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg ctacggcata cagccgggat
    48601 gcattacctg ggtgggtgac gatgactacg gcacaaagct gaagcaggat ctcgcccgca
    48661 tgggcgtcga tatcagccat gtccatacga aacacggcgt taccgcacaa actcaggtgg
    48721 aactgcacga caatgatcgc gtttttggcg actacaccga aggcgtgatg gccgactttg
    48781 ccctgagtga agaggattac gcctggctgg cgcagtatga cattgtgcac gcggcaatct
    48841 ggggacatgc ggaagacgca ttcccacagc tgcacgctgc gggcaaactt accgctttcg
    48901 acttctccga caagtgggac agcccgctct ggcagacact ggtgccgcat ctcgattttg
    48961 cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct gaagatgaaa gcgattgttg
    49021 cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg
    49081 gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt tatcgacacc atgggtgccg
    49141 gagattcgtt cattgccgga ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg
    49201 cgatggcgca gggaacggcg tgcgcggcga aaaccattca gtaccacggt gcctggtagg
    49261 tataacgttg gcgtgagcat cttcacgcca acgtgctgtt acttgccgga aaacgaccct
    49321 ataatccgag taattcattc tttatttcag ggtcgattat gtcagctacg gaccgctact
    49381 ctcatcaact cctctacgct accgtccgcc agcggctgct ggatgatatc gcgcaggggg
    49441 tttaccaggc cgggcaacag atccctaccg aaaacgagct ttgtacacaa tataacgtca
    49501 gccgcattac cattcgcaaa gccatcagcg acttagtggc agacggcgta ctgatccgct
    49561 ggcagggaaa aggcaccttt gtacaaagcc agaaagttga aaacgccctg cttactgtca
    49621 gtggttttac cgattttggc gtctcacaag gcaagtcgac gaaagagaaa gtgatcgaac
    49681 aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa catccccgga aacagcgaag
    49741 tgttccatct ctgccgggtg atgtatctcg ataaagagcc gctgtttatt gatagttcat
    49801 ggatcccgct gtcgcgttat cctgactttg atgagattta cgtcgaagga agctccacct
    49861 atcagttatt tcaggagcgt tttgacacgc gagtggtcag cgacaaaaag accatcgata
    49921 tctttgccgc cacccgcccg caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt
    49981 ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc ggtgcacgtc tccgaactct
    50041 tctgccgcgc caatcgcatc accttaacga ttgataataa aagacattaa ccgtaggccg
    50101 gataagatgc gccagcatcg catccggcga tgctggcgca ctgaatttta catcccgcac
    50161 gttcccctca ccctaaccct ctccccaaag gggcgagggg accgttcagt gcggaattta
    50221 gcttgattgc cgaacggtgc tatccagcat taaatactcg caatactctc acgcaaaatc
    50281 cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcaca atgttcgagt
    50341 tgtggattcg cctggcgaaa cgttcctgaa agtcgataga agagcggcgg gatttcatat
    50401 ttcgactctg caccgaccgg gtaaggcaac gcgccacgct tttgcgcttc ttccagcacc
    50461 ctggcggcaa tcagctgatg aaactcgaca atcaacacct tcgactgcgc attggcaatg
    50521 acggcgcttt tcacttccgc caccgcgccg ccgttaagca aagccagcaa ccgttcggat
    50581 actcccgcct gcaccgcatg catcactggc gcgaacacca gaccacgcaa gacttccagc
    50641 gcctgtgagc cctggatctg gctaccgccg gagtaaagcg tggcgcgaat ccgactaata
    50701 acatcggcat cgccaaccac tgcaccaacg ccccccggcc caaacagctt gaagcaggag
    50761 aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ctaccttcat caccgcatag
    50821 ttatcatcgg ttaacgctgg aacacctgcc gcgcgcaacg ttgccagcac atctgccagc
    50881 acatagctgt cctgcggctg ctggcgcgta tgctgcacca gcgcggcatc aggttgttgc
    50941 tcgtcgacga cctgcttcag tgccgacagg tcattgaaat cagcagtaat aagcgtcagc
    51001 cccatctgct caataataac ccgtgtcgtc gggtaaacag gcgcgtcatg caccagaaga
    51061 cgctgccccg gtttgagcaa agccgccagc ccggcgcgaa tcgcgccagt ccccgcgccc
    51121 tgcaccagcg ccgcggcctg tgcgtgaaat gcatcagcca gcacctgctc cacccgttgg
    51181 gtaatacgcg gttgattcag acttggagtt aaccctaaat caccgccagc aagaaactcg
    51241 ctgcctggaa aatggcggca aatagtgtcc accagcgcaa actgcttttg ctgcgcttca
    51301 gcaagagtca ggctttgcaa aggaaacgtc ttcataggtg ccttccttaa gccggaacga
    51361 acaggccaag ccagtaaaga acattcagca gaataccggt gatcattact gccacgaccg
    51421 gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa atggcaaccg
    51481 cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa ccgaccagca
    51541 gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca ttacgcaccg
    51601 acgggtagcg tcccagccat ttgccgatcg aacgaagcag caagacttcc gccgaaataa
    51661 ccactgcgcc taataccgct gcaaccatcg gattcggcga gagatagccc accgcataaa
    51721 caaaggtaaa gcccgcaact gcatacacac ccgttgctaa ggcggtggtg gcaatcaacg
    51781 gcacaaaccc cagtccgcgc ataaattctg ccagagccgc ctgattaatc agcgtttgcg
    51841 attgttccgg cgtcacgcct gcggaatacg ctttctccag tgtgaagatc gaaacttcgc
    51901 taccagcaaa aatcttcata ctggcaacgg cggcaatcaa tgctcccacg atggcgatat
    51961 aaggtaagtt tttgataatc cgcgacgtgc gctcttcaaa caccgaaagc ccgctggcat
    52021 cgatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc agcagcatca
    52081 ccatgccaat aaagatttcg atggattcag ggttaagatg tgggaaatag cgcacgacta
    52141 ccacacgggt catcagaaca accacggcgg cgaccagact ttgcttccag ccaaactggt
    52201 agaaaatcgc caccagcggg aacagtgcaa aagcagaaac caccggcgag cttaattccc
    52261 cgaggctacc taatacatcc accggcagcg cggtcagcag ctggttaact ggcaacaggc
    52321 aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg ctgtttatcg
    52381 ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttgagc agcccggttt
    52441 tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca aaagcgatac
    52501 tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc gggtggatgc
    52561 cgtcatgaaa aacagccgcc gagcgatgcg ccagaagcga tgtcataccc gtcaggcacg
    52621 ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca gatggttaat
    52681 caacatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca ctttcccttc
    52741 agcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg caatggtgca
    52801 acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg caccggtatt
    52861 acacgcgcca atgtaataat ctagttgccc ggatttcacc ttcattgccg cttccatgtc
    52921 gttgtgaatg aaaacttcaa aacagccagg agccgtcgct tcaatggttt ttttaatctg
    52981 ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt ctgtccttat tggaaaaatt
    53041 gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcgca
    53101 attgcggaat aaaggtggtc aataaatagt catagccata accaccattg gcttttaaat
    53161 gggagcggcg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca cggtcacgta
    53221 gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta actgttctta ccaatggtgt
    53281 cgaactgcac gtacgcgccg agatcgatca tcttcaaaat gttgtcgagg ttgtctttca
    53341 gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca
    53401 gttgttccag ccccatggtg ctgaacgacg tatgcgtgga gatcgggcgt ccggtctggt
    53461 tatgcgccag cgcagcggca ataaatacct tctcttccag cggcgtaatc tttccttcgc
    53521 tggagccgat ctccgcgatg atcccggctt tcagctccgt gccatcgata ccctgttcaa
    53581 tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcaccaca tgttccggga
    53641 aaaacgcatc ctggtaataa ccagtacagg ccaccacgtt gatcccggtt tcgcgcatta
    53701 catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca atcacattac
    53761 gtacaccccg ggtcatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc
    53821 ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca tgggccaggg
    53881 tgtaacccgt cggatcaaaa ctcatagggt gtctcctgca aaccggaggg aggaaagaaa
    53941 agagcgacca ttttgtggcg gtggcgcacg gaaaaattca cacacggtag ctcccacatc
    54001 agaaagcgtg gtgcgcacac cgagctgcgt ggcgaccagc ccttgctgat aaaccagcac
    54061 tggcaccact tcacgggtat ggtggctgtg accaatggtc ggatcgttac cgtgatccgc
    54121 catcacgacc aggcaatcat ctggctgcat cgcctcaaca agccgggcaa ggttacggtc
    54181 aacgacctgc aaacgttcgg cataacgtgc gacgtcttct gcatgaccag cgaggtcggt
    54241 ttcctggata ttgatgcaaa taaacgccgt cggatgggtg ttaaattcgt cgagggtgat
    54301 atccataatc cgctggctat ccaccaggtt ttgccagctc acaccataag gattgctgac
    54361 gatatctgcc accttaccca ccagcacggt tggcacgccc gcttcataca gtttttgcgg
    54421 cacctgcact ttttcatcaa caccgtagcc catatgcacg acctggaagc cgttgtcata
    54481 agcgccagaa cgcggcgcat taataccaat aaagcgccct tctttgcttt ctgcggcatc
    54541 gagaatacgc tgactgttgg ttaacaggcc gccaaatgta atgacccgac cgacctgtac
    54601 ctggtcacgc acgatacgac caattttgat tgcgtcgtca aaagagatca cagagagatt
    54661 ggcggtaatg ttatagactt ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc
    54721 ctgattgacc gacagaaatt gcagatcatc gccacggcgc tccacctgcc agccagcgga
    54781 aactaatgcc tgctcaacac ggtcaatcac atcgcgaaaa ggcatccgca gcggcggtaa
    54841 cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag
    54901 ctctgccacg ccccaggttg cggaatctga cggctgcata tcgcctggcg cataacccaa
    54961 tgtgttgatt agccccagct tctccagcgt tggtagctgc aaatgcggca actggctcag
    55021 gatgtgacca caggtattcg ccccggcatc ttgcgggcgc accagcgtga catctttcat
    55081 tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccattt atgcctccag
    55141 agggttgccg aggctgtcat aacgaccaac tatttctggt tcgccgcgct gaataccgga
    55201 caccagcacc acatcgctac gggtgacaaa aatctgcgtg cgaaaacaga gcaccactgc
    55261 actgctcacc ggatactcgc ctgccagcgg cagggtgtag tcgatactgc tgtcatccac
    55321 agttttgaga ttggtttcag taatcttttg attttctggc gtaaacacca gcgcatgttg
    55381 cgcatgacca cgacggtagt aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg
    55441 ggagatttca cttaaccaga gcatcgcgat acgttcaggc tgatcgccct gctggtttgc
    55501 cggaatagta ccggtcagcg catgaccggg ttcggcatga gtcgcaccgt attccgccag
    55561 caatggcagc gaagtgcagc tggtcgctga aggtacgttc agttgctcaa tcgcaatacc
    55621 agatttcgcc agttgatccc gtgcttgtac cagcgtgtga agattcggtg ttggcaaaac
    55681 tttcccagca gcctcatccc aaagcaggca agggaaatgg gttagtccgg ctaaatgcag
    55741 ccccggcagt ttcttaattt cagcaatcac ctcgtgcagc gaatgctgta caaaaccgct
    55801 ctcctgcccc ggataaagaa aatcatcgtc gctataaact ttgagcagca caggctggac
    55861 ccgtccggcc ttaaccgctg ccgcagaaac ttcccgcgct ttgtcgagag taaacacggt
    55921 gatgatgtcg gtgccctgtt caacggcgtc agcaacctga tgacaaggga tttgtaccag
    55981 atgcccctga tgcgccactg gcaaaccagc gcggcgcatg actcgcgcct ctttgtaatc
    56041 caccgccaca atgccgctgt agcctaatgc cagcaatttt tccgccagcc acggattacg
    56101 accaaattgc ttggtcatca gatacagttc aatgccgtaa agccgcgccg tctcaatgag
    56161 ccgcttaccg ttttccagta tctgatccac gtcgatcacc cagctgtccg gggcgatttt
    56221 gccctgctgc cacaaactta gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc
    56281 ttctacaaac atcttgttgc ctcgtttctg ttccttgaat taaatattca tttttttgaa
    56341 tatttaggtt gtgcgacgtg gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc
    56401 cataatccat aaagattggc cagaaaatag ccttcttcac acgggttaac ttccagagcg
    56461 aattccttca acaactcctg atgcaattgc acaacggctt gccagtggct ggattgcgcc
    56521 agttccgcca gcaactcgtt atccagcggc tctatttctt caccacggcg actgcgcatc
    56581 agtgcactcg ccatatgtgt catcgccatc gttccttgct cactgcgcac cggcagatgg
    56641 cactcttttt ccagtacgtt gacgacctgc atcatgcctt tgcagacgtc cttatcaata
    56701 acgcctgcct cgcaaagcag gttgagtctg gtttccatat cagatgcctt tcgtttaata
    56761 actgggttcc tgttccccac tcaccacccg ttgttgatgg gcaagcaggg cgtgtttatc
    56821 tacaacagca cgcagcaatt gttgcattgg gtaatcatcg actcgcgtca ggaccacggc
    56881 ttcggtggcc tgtaaaaatc gcggatcgtc tgttagcggc gtcgcctcta atcccagcat
    56941 ggtcagttcg ttttccgcca ccacgttcca gatcaccgca tcgacatcgc ctttaacaat
    57001 gcgttgtaaa ctctcgtgat aagagagatc gactcgttcc acatcactat cgccaaaaaa
    57061 gacatcggtc atgattttct gatccgccga acgactatcg agacctactc gcttaacatt
    57121 tccagactca cctttgcggc atatcagttg atgttcacca acatacgtat gcgggccaag
    57181 ctcgagggcg atgcacaaat tgttttggct gagataactt tccgccgcca ggcgggaaac
    57241 tactgccatg tcatagacac cgttaagtaa acactccacg cgaatatccg cgccgcgcat
    57301 atgcgcatag taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg ctaaaccttc
    57361 gtacaagcgg gtatagggca aaggcatcgc acataccacg ttgttgatat ccacatgact
    57421 cagcaatgct ttgttatcca tctcgacaag ataactgcca ttgcgcccac ggcgttcaat
    57481 ccgtatcgct ccgcttgatt ccagcgtttt caacgcggcc tgcgtcaggc caaccgatga
    57541 acggcattca gttgccagct catctatggt tttcagtcga ttaccgcatt tttcacccaa
    57601 caaataacgg gccagtgtcg tgatgacgac gccttctttt ttgataaacg ttcgacgcat
    57661 aatatatttt caataaaatg aatatttata tcttcattaa aatgaagata agtggcgacg
    57721 acgagatgtg gaaacggcga ggcgcttcac attttttcgg gttgttgcga aaagaggata
    57781 aaaaaaccgg gtttccccgg tttcagagtg atgacgaagt gatttattcg acaatgcgag
    57841 caaagggtaa ttcccactgg cgcttgcctg ctcccctcac cctaaccctc tccccaaagg
    57901 ggcgagggga ccgtccgagc gttttgatat tacggcttcg ccacaaaacc aatcgcttcg
    57961 tacaccgctt tcagcgtacg ggaagcgtgt acactggctt tttctgcgcc gtctttcatc
    58021 acctgttgca ggaaggcttc atcgttgcgg aaacggtgat agcgttcctg caattcagtc
    58081 agcataccgg aaacggcatc agccacttca cctttcagat gaccatacat cttgccttcg
    58141 aactgttttt ccagttctgg gatgctctgg cccgttaccg cagaaaggat atccagcagg
    58201 ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca
    58261 gtgaccgcgc gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg
    58321 ttattgcggt tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg
    58381 cgcgcgccag atttcggaat aaacggctcc ggcaccttaa agatctcgcc atacagcgcg
    58441 ttgaaacgct gggcgatatc gcgactcagt tccaggtgct gtttctggtc ttcacccacc
    58501 ggtaccagat tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagccaaac
    58561 agaccagcgt tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcga
    58621 ctcagttcgc cgaagtaggt atagcagttc agtgcccagc ctaactgcgc atgttctggt
    58681 acgtgggact gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag
    58741 gccagcgtat ccaacgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg
    58801 tgttggtcaa cgatacagta aacgcaatgg tagtcatcct gcatgtttac ccactgacgc
    58861 agcgcaccca tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg
    58921 atgggcttag tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag
    58981 aaggtcattt atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta
    59041 gttgtatccg taggttaagc caactgatgg gcaacctgcc gcttttgccg cctgaatatc
    59101 attccgtgag tcgccgacaa acagcatctg ttgtggggca attcccatcc gctcagccac
    59161 cagtaacagc gggtccggat gcggtttttt gttttgcaca tcatcgccgc cgataaccac
    59221 gctaaagtat ttggcaatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt
    59281 gttggtgacc aagcctagcg gcaggccttt agcctgcaac gcgcccaacg tatcggcaac
    59341 gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca tagtagcgat cgaacagttt
    59401 acgcagaata cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttccccat
    59461 tgttttacgc agagtcgcac gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac
    59521 atctgcgccg ttaccaatcc aggtaataac gcgttcttca cctgcgacgg gcaactccag
    59581 cgcatacagc gccatatcta ccgcagcagc aagaccagga gcactgtcga ccagcgtacc
    59641 atcaagatca aaagcgacgc cgcgaatatc ttcaaactta ttcatgactt acctttgcca
    59701 gttcactgcg catttcatca atgacttttt tatagtctgg ctggtcgaag attgccgaac
    59761 cggcgacgaa catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc
    59821 caccgtccac ttccagacga atgtcaaagc cagactcgtc gatacggcga cgtacttcgc
    59881 gcagtttatc cagtgtttga ggaatgaaag actgaccgcc gaaaccaggg ttgacggaca
    59941 tcagcaggat cacatccagc ttatccatca cgtaatccag atagctcaga ggtgtcgccg
    60001 ggttaaatac cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa
    60061 catgctcgga ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag
    60121 gcacaatgcg atcgacgggt ttcaccatca gatgtacgtc gataggggcg gtaatgccgt
    60181 agttacgcaa ggatttcagc accattggcc caatcgtcag attgggaaca tagtggttat
    60241 ccatgacgtc aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac
    60301 ccaggcgggc aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct
    60361 tctccttgag aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag
    60421 ttcgtccacc tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac
    60481 atgcaatttt gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag
    60541 cactggaata tggcgctcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc
    60601 aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggtgcata
    60661 aggcggatcg caatagacga cggatgcatc atctgcgcgc tccatgctat cggcgtaaga
    60721 ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg
    60781 gaaatagggt tttttgtagc ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg
    60841 acacaggccg ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg
    60901 atcctggctt ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc
    60961 gggaacaaac agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt
    61021 atagagactg atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt
    61081 gagaaacacc gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg
    61141 ccgtttaata tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt
    61201 tttcttcatg ccgattaact aattacacct tctccggctg tggagaaagc tccgacagca
    61261 tccagcgctt caggcagctc ccgcaacgat aaaccattgc gggagataac cttgattact
    61321 tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tgaacatctg
    61381 ctggcaatgt agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca
    61441 ggacatacca cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct
    61501 ctttcttcgc ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt
    61561 aatggctgga cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg
    61621 ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct
    61681 cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg
    61741 gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg
    61801 gttttttcgg ttcaatcacc gcctgctgac gaactgggcg cgtagtggac ggacgttcag
    61861 cggtttgcgt tttcgccgag tcacgcgatg cattgccatt gcgaacaggc gcaaccgttg
    61921 cgggttcagt cggcaacgtg gaattgaccg ccacattgtt cagctgttgc tgattttgtg
    61981 gctgggtcag cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg
    62041 ccaccggggt ttgcccttga gtcggcgtag aagagatcgg tggcagcgaa acatcctgct
    62101 gagtattttc cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg
    62161 cattgccagc aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg
    62221 agggggcttt tagcgcagaa ccgataccga tgatcaacag cagtagaacc agaatgccga
    62281 cgcccatcat catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgcg
    62341 gacgacgctc tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttcttctt
    62401 cgatttccgg ttcctcattg cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt
    62461 catcaagttc aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac
    62521 gttcagaaga ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt
    62581 cttctggttt gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc
    62641 aattaacctg aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct
    62701 gattgacaat cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca
    62761 ctcttaccaa ttgccaacgg aagaattaag cgcatctctc ccgcaaggac tttcttgtca
    62821 cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac
    62881 ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga
    62941 cgttccgacg tccgcgccgc catcaccata cccgcagcga ccgcttcacc atgtaaccaa
    63001 ttgccatacc ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa
    63061 gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt ctgccttcag ttcacaacaa
    63121 cggcgaatac agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct
    63181 tccagccagt taaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc
    63241 cccgacgcta actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa
    63301 gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg
    63361 ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca
    63421 ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc
    63481 gccaccagcg tagtatcgcg accatgcggc ttttgtaaca acgccgtaaa gacggtatcg
    63541 agtacagcta ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca
    63601 cccgcctgtt caagtacgcc gcggacctta tcgagataca gaggagccag ggtttcgctg
    63661 gtgaccaaca tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac
    63721 aaaccagatg cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc
    63781 ataacgcgac atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt
    63841 agttgctttc cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag
    63901 tacgaatggt cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt
    63961 ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg
    64021 tgcgtgcaag ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa
    64081 gacggttacg cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct
    64141 gtttctcggt caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt
    64201 ctaaatcgaa aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat
    64261 cgtaaaattc catattgagc tgttgagcta actggcgccc aatagtgctt tttccggcac
    64321 ccataggccc aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac
    64381 tattcgttaa tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct
    64441 cataagatat tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac
    64501 tgaataaatc acccatccct ctgcatatct ggtcgctgca agcgcgctgc cttgctacca
    64561 ccgctctggc gataaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct
    64621 gaatgataaa tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta
    64681 ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcataaaaac
    64741 attaccgctt ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact
    64801 aactcgcgtc gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg
    64861 tcgccaagca acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc
    64921 agcgccaacg tttctccgct ttttacctcg acctgcgttt cgatctcctg cttatcaatc
    64981 gccagcactt cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt
    65041 aatttcagcc ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggacg
    65101 gcctctttaa attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt
    65161 tcgctctcct gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg
    65221 ataatatcca gctgctgttt ttgttcgagc gcggaaagct caagatccag caagcgtccg
    65281 ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgcta
    65341 ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg catcggccag cgtccatttc
    65401 acgcctaact cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc
    65461 tgcccgaccg gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg
    65521 ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggcactc
    65581 agtagcttct cccccgcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg
    65641 cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata
    65701 ttgtcattct gccaggcaat ggaatgcact gagagaatgt tgccttcctg ccgcgttatc
    65761 agtccggcgc ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt
    65821 aacgacaccg taccgctgac gtctggcgac acgaccaggt tcaacttctc ctgttcagcc
    65881 agcgcctgca acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc
    65941 tttgccgcct gtacgccggg tatcagcatc aacagtagtg cggctatcca ttgcttcatt
    66001 tgtatctcct tgccgttgcc acaaccattg tggcggttcg cagtttgtcc cggtacccag
    66061 cgttagtacg tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg
    66121 ctgcacccgt cgccatttct tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg
    66181 ccctaccatc ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg
    66241 cgatttaaaa gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca
    66301 gcgtttaacc ctcatgcggc atctccagtt gtagcgtgaa caaaagatca tcaccttcca
    66361 cgcttaacga aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg
    66421 gcactgcttc ccacagcgtt ttcaacgcca actcgcctcc ctgcgcggat ggatgccagg
    66481 aaaccagttg cgcgccggat aactgaaaat ccagtggcga aaagggcagc gttttttcct
    66541 cgctgaaggg agtggtgtct accaggcgat acagtgcggc ccactgctgg tgatgacttg
    66601 cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt cgatgataga aaaatgagcg
    66661 taactaacat cagcaaccag aatgcccagc aaaactggcg gaggcggggt gatgtggcga
    66721 accaccagtc aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc
    66781 cagcgtccct gcgcatcctg ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc
    66841 tggcggagtg acgtttctag tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc
    66901 tccagcgttc cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc
    66961 gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc
    67021 gaacgctgtt cgcgtaactg ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg
    67081 cgggcgagtt gttgttccgc ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct
    67141 gtcagacgca gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc
    67201 aaccagaaac gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc
    67261 gggttcatca gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccaccag
    67321 gcggcggtag cggcggctga cgaacagaaa cggcctccca ggggtcaaat ccgccttcgc
    67381 cacatatcgc gacgctgtcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag
    67441 tcatccccac cgccagtttg cgcccccagc tatagcgcgt cgcccacagc cactgttcgt
    67501 tatcacgcca ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg
    67561 cactggcatc cggggtgatc gcactaacat gaacacgcaa cctttctgcc agcgtaagca
    67621 gcgttgccag ctctttgctt tgcgcggcag tcacgttata agcggggctg agtgagtctt
    67681 cgctgtaatc gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtccctg
    67741 acagccaggc cgtttgctcc cgctcaccaa gggacatcga cgggcgcgga aatgaccgct
    67801 gtaatgtgcg actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac
    67861 tccacggtaa caacgtttta gccagctgct gcgcatcaac aatccgccca tctttgataa
    67921 tgtcgtgcgc cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgca
    67981 cgatcgcaac cgctgccgct tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa
    68041 atgtcattgt gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg
    68101 cttgccttta tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa
    68161 tttccagtga agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga
    68221 gcaggctcga tttatggcct ataccgctac atcgagccac aactgccgga tgtggtgaca
    68281 ttaaaagatg ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt
    68341 gctcaatacg gtgagaaacg tcgtattccg gttacgttgg atcaaatccc accggagatg
    68401 gtgaaagcct ttatcgcgac ggaagacagc cgcttctacg agcatcacgg agttgacccg
    68461 gtggggatct tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg
    68521 agtaccatta cccagcagct ggcgagaaat ttcttcctca gtccagaacg cacgatgatg
    68581 cgtaagatta aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgaa
    68641 atcctcgagc tttatctgaa caagatttac cttggttacc gcgcctatgg tgtcggtgct
    68701 gcggcacaag tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggcggtg
    68761 atagccgggt tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc
    68821 gtcgcgcggc gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccagcaa
    68881 cagttcgatc agacacgcac tgaggcgatt aacgctaatt atcacgcgcc ggagattgct
    68941 ttctctgcgc cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa
    69001 agtgcctatg aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc
    69061 gcgcagcagg cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc
    69121 ccggcaaatg tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat
    69181 acgctgaagg cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat
    69241 cctcagggag cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc
    69301 gttcgctggg cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg
    69361 accgatgttc tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg
    69421 ctggcacaag tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaatggtgcc
    69481 gttatggcgc tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag
    69541 gcactgcgtc aggtgggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa
    69601 ggtctgacgc tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc
    69661 ggttctgact ggcagccgaa gaactcacca ccgcagtatg ctggtccaat tcgcttacgt
    69721 caggggctgg gtcagtcgaa aaacgtggtg atggtacgtg caatgcgggc gatgggcgtc
    69781 gactacgctg cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc
    69841 gaatcgctgg cgctgggttc agcgtccttc accccaatgc aggtggcgcg cggctacgcg
    69901 gtcatggcga acggcggctt cctagtggac ccgtggttta tcagcaaaat tgaaaacgat
    69961 cagggcggcg tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattcca
    70021 gtgatttacg gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc
    70081 gctatctccc gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat
    70141 caggcgttag tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg
    70201 ctggcattcc tgattaagag tgctttgaac accaatatct ttggtgaacc aggctggcag
    70261 ggtaccggct ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt
    70321 accactaaca gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg
    70381 gtctggattg gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg
    70441 attaaagatc agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac
    70501 gcttatatga aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt
    70561 attgtgacgg tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc
    70621 gaagagtatt tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg
    70681 accattatcg ataatggtga ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc
    70741 ttttcagttg gctgacaaag tgcatttgtt tatgccggat gcgggtgaac gcgttatccg
    70801 gctaacaaaa tcgtgaaaaa tcaatatatt gcaggaaccc cataagcctg ataagcgttg
    70861 tgcatcaggc aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg
    70921 aggaacagcg cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc
    70981 atatgcgcca gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat
    71041 tccgggtaga gatcttgcgc gaccacgata ttcattttgc tggaaaagta agacggtgcc
    71101 atgctgagct tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc
    71161 tcgcggttag cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct
    71221 aattcgtagg attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcatccaca
    71281 atcggcacaa tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc
    71341 acgccattgc tgaactccag atccacgctc tcgacggtaa acagtcggga acgggctaca
    71401 gtttcaacat tcagaatggt gggtttttgt aatgatttgt tcatcgtggg atctatgctg
    71461 tgaaatcagc ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta
    71521 tttacattta tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt
    71581 gaaataactc caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt
    71641 tactgtgatg tgcttaatga tgctcaagtt aagctccacg cttgccgata gccaaccgca
    71701 gaatcatgta ttgtgtccgg tgcgactgac cacgcctgac agactaagta agatggggaa
    71761 agcatgagca ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga
    71821 tggctgataa aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat
    71881 gcgcaaacgc gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc
    71941 ctgacgcagg tattacaggt gcctggccca acgggagcta gcgcggcacc gatctctctg
    72001 gcgctgaatg ccgaaagcaa caacgtcatg atgctgacac acgctatcac acgttacggc
    72061 atctctaccg acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg
    72121 ccccccttct gggaacagta catcaacgat gagaataccg ttgaactgat tcataccgat
    72181 tcgctgccgt tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg
    72241 cgcggctatg ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt
    72301 gagcaaatcg agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt
    72361 ccgcgcgggc tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc
    72421 ctgattaccc cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc
    72481 gcaggtctgt gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt
    72541 ctgcgcggta caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac
    72601 aatatttcac tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct
    72661 caggatctcg gtcaacaaac cgatattgat atctatctcg accgccacgt agtgcgtcag
    72721 gggcgatatc tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc
    72781 agtacgatta tcgctgcggg ctcgctgctg gtgctgttta tgctgttatt ctggatcccg
    72841 ctggatatgc cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc
    72901 accagcgtaa aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc
    72961 ggtacgggaa tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct
    73021 tttttaccgt ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca
    73081 gaatctgaac tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac
    73141 cctaaaccgg aagatgaatc tcgcgtcagt gcctcattgc gttcagcgat tcaaaaatcc
    73201 ggcatggtat tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct
    73261 gccaaagatg actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac
    73321 tgggacgcgc tggtaaaacg cgccaacgcc gggaagctcg atggcgtgaa tgtgttatta
    73381 cgcccggtga gtgcggaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc
    73441 acgcatgaaa cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg
    73501 attgtcagcg acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac
    73561 gactacccgc cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat
    73621 acgccgttta acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag
    73681 catattggcc ttcatccgat cccggatcgt tccggcctgt ggcgctatct cagcaccaca
    73741 ttgctgctac tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt
    73801 taccagcgtc atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac
    73861 ccgcaactga tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat
    73921 gctaccctgt cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc
    73981 ctggcaggac gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt
    74041 cgataactat ttctggcaaa agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac
    74101 gccacaggaa gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa
    74161 ctggtactgt cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac
    74221 cgagatggga ccgcgcgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa
    74281 agccagcggt aagcagcgaa ttttgctcac caatgctcat ccgcacaacc tggcggtaaa
    74341 acttgagcat acgggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg
    74401 ttatccgaaa gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc
    74461 tgaaagaacg ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg
    74521 tattcgttac tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca
    74581 acgccatccg tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg
    74641 ccatgaaaga gaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt
    74701 tttataaaac ccgcgcgctg gcccgtgaaa tgattgaagg cggtaaggtg cattacaacg
    74761 ggcagcgcag caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg
    74821 gaaatgacga acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg
    74881 aggcagcctt gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga
    74941 tggcacgtaa acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag
    75001 agcgccgcga cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag
    75061 agatgattat gccgcaacat gaccaattac atcgctatct gtttgaaaat tttgccgttc
    75121 gcggcgaact ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaac cacgattatc
    75181 cgcagcccgt taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta
    75241 cgctgaagtt tgatggtgat atcaccgtac agctgcaggg cgacggtccg atgaatctgg
    75301 cggttattaa cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa
    75361 ttccagaaaa tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta
    75421 ccccgagcga aggcgaacgc tatcagggcg tagttggtct ggaaggtgat accctggcgg
    75481 cctgcctaga agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca
    75541 ccggcgacgt agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc
    75601 aaaataccca acaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg
    75661 aagaactgct gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg
    75721 tgacggttta cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg
    75781 ccgatgcgct gaaaacgctg cctgatgaag aagttgatag catcctggcg gaagatggcg
    75841 aaattgacat gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg
    75901 ctgaaatccg caacaacgcg tctccggcag atccgtaagt tcattaatga gtatgtccgg
    75961 cagagagggc tctgccggat tttacgactt aatggcctga tgtgctgcgc ttaccagcgc
    76021 ctatgaaata aatcgcaaat tattttggtc agatacagcg tcgtatccga taaaagcgtt
    76081 gtcacttact taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt
    76141 tcggcctcgt ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt
    76201 cgttcggaag taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat
    76261 agacaatgac tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat
    76321 tcagatcttg ctcgaccagt acgcaggcgt ctttttcaat acaggtcatc acgttatagc
    76381 gatcctggat taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta
    76441 acacattttc ggcgaatact ttttccgata cctgttcctg aagatgttgc tctccatcta
    76501 acgccatcag cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca
    76561 gcgactcaag cgccgcacgt ccatagcgac cgctctgttc gagcatgtag atagttacct
    76621 ggtctggcgt gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca
    76681 tactgtccag caccggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg
    76741 caaggttcac tttgccctga agaacaaggg gattttgccc attacgccag acgatactga
    76801 caaaataccc cagcgaccac accagtaaca ccagctccgc cagcgcgccc tgcaagcggt
    76861 cgacagtcca gccgtactga gcgacccgca gccataaccc ccaggtggcg acgaacacat
    76921 acagcggagc aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa
    76981 gtgacgcttt ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg
    77041 ttaacagcaa cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca
    77101 gggtgatgat aaacatcggg gttagcaatg atactaacgg cagcaacccc gtggcgatta
    77161 gcgtgaacaa cttttgaata gagtcgatta aacgtgactg tgtacgcgcg aggatcaccg
    77221 ccagtgcggt aaccagacca aatgtaagat acataaacca gtcggttgca aaaaagagcg
    77281 tattgaaaaa cgtgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccagcc
    77341 acgtcaggcc attggagaga aaaatcacca gtaatataag tacgttatgc catactgact
    77401 ggtaaaagta gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg
    77461 gtagcaacaa cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttcag
    77521 ctctccaggg gttcatgcca tcagtttgcc acttcagcca accgctcatc cctgtcgtgg
    77581 caataaacac cagcgccagc catccccaaa ggcgcttctg tttaaaagag atcacggaga
    77641 agagtaaaac ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc caactgtgat
    77701 tttttccggc aagccaggcg ataagcaggt agcaaaccag cccctgaagt aatccggtag
    77761 caatcatccc ccagcgtgtt gccggtgaaa gttcaacgtt atccatgttg gttatccaga
    77821 atcaaaaggt gggttaatta tcgcatccgg gcagtagtat tttgcttttt tcagaaaata
    77881 atcaaaaaaa gttagcgtgg tgaatcgata ctttaccggt tgaatttgca tcaatttcat
    77941 tcaggaatgc gattccactc acaatattcc cgccatataa accaagattt aaccttttga
    78001 gaacattttc cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga
    78061 atcacggagt tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc
    78121 gcggttaaca cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag
    78181 tgaaatgcgc gttaacaatg gtttgccccc gcaagaactc gaggcttatg gtatcagtga
    78241 cgtacatgat atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc
    78301 gagcctgaca ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac
    78361 cgggatcttc accggtcgtt caccaaaaga taagtatatc gtccgtgacg ataccactcg
    78421 cgatactttc tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc
    78481 ggaaacctgg cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt
    78541 cgttgtcgac gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac
    78601 cgaagtggcc tggcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga
    78661 gctggcaggt ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca
    78721 gtggaaagaa cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat
    78781 gcagctgatt ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat
    78841 gaactacctg ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga
    78901 gaaaggcgat gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac
    78961 cgacccgaaa cgccgcctga ttggcgatga cgaacacggc tgggacgatg acggcgtgtt
    79021 taatttcgaa ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga
    79081 aatctacaac gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg
    79141 cactatcgac tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccaatcta
    79201 tcacatcgat aacatcgtta agccggtttc caaagcgggc cacgcgacta aggttatctt
    79261 cctgactgct gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac
    79321 ccagtatcac ttcctctctg gcttcaccgc caaactggcc ggtactgagc gtggcatcac
    79381 cgaaccgacg ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac
    79441 tcagtacgca gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt
    79501 taacactggc tggaacggca ctggcaaacg tatctcgatt aaagataccc gcgccattat
    79561 cgacgccatc ctcaacggtt cgctggataa tgcagaaacc ttcactctgc cgatgtttaa
    79621 cctggcgatc ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac
    79681 ctacgcttct ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga
    79741 caacttcgat aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa
    79801 actgtaatga tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga
    79861 ggcaccttcg cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg
    79921 gcactggcag ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca
    79981 tcccgttatg gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa
    80041 tggtgcgcgc actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg
    80101 caattcccgg accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc
    80161 tgactttgat ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc
    80221 gtttgatcga cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa
    80281 tttcccgctc atagccactt tcggcagcaa tcacctcacc gagcactgca ttaagatccg
    80341 ccatttccat cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg
    80401 cgttgcactc ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca
    80461 tcatctcagt cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg
    80521 ccatcagcag cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg
    80581 cacgggtaac ggaacgcacc tccgaagcgc catattcacg cagcggcggc ggaataatcc
    80641 ctttaccaac ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa
    80701 taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg
    80761 gagagaaatc gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca
    80821 gccaggtttt cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg
    80881 ggccgcccag ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca
    80941 gacctgcctc ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct
    81001 cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg
    81061 tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa
    81121 tcgcgaagtt cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga
    81181 tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt
    81241 agagccgtcc ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg
    81301 cgctggatct tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
    81361 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
    81421 acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg
    81481 ctcatcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
    81541 taccgcctct tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc
    81601 acgaatacgg gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc
    81661 aatctccagg cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg
    81721 catcgggttg ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
    81781 cattaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc
    81841 tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc
    81901 acgcaggcgc atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac
    81961 tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc
    82021 agctaaatct ggtataaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta
    82081 agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca
    82141 atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc
    82201 gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg
    82261 aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact
    82321 atcagtataa taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat
    82381 gcctggaaaa tctcaaaatc gtcgattact cccctcagca ggaaggcaaa gtattttttg
    82441 gcgcgtgggt ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct
    82501 acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat
    82561 tgctgaaaaa agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgaact
    82621 ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat
    82681 ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat
    82741 gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc
    82801 gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta
    82861 tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg
    82921 aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca
    82981 tttccgagca aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca
    83041 aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa
    83101 tcgccattga agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca
    83161 cgccagaagt cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag
    83221 ccgcgctgga tggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc
    83281 tggcgaaagt gcgtgattat ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg
    83341 gtaaagaaga ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgttgtcca
    83401 cggtgccttc tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc
    83461 tttcgctgaa tgccgatccg cagttcgatg agccgcccaa agagagctat tgcgagcaaa
    83521 tcatcatgga tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaaa
    83581 gcgtagtgag ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg
    83641 gtaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg
    83701 atctgctgat ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc
    83761 gtaccggggt aaaagtggcg gtggtcgatg ccactggcaa actggtggcg accgatacca
    83821 tttacccgca caccggacaa gccgcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa
    83881 aacataacgt tgaactggtg gcgatcggca acggtacagc ttcccgcgaa actgaacgtt
    83941 tctatctcga cgtgcagaag cagttcccga aagtgaccgc acagaaagtg atcgtcagcg
    84001 aagctggcgc gtcggtttac tcagcttccg agctggcagc gcaggagttc ccggatctcg
    84061 acgtttcgct gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc
    84121 tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga
    84181 cgcaactggc ccgcaaactg gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg
    84241 atctcaacac cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg
    84301 cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt cagcaactgc
    84361 tgaaagtcag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta
    84421 accacggtga taacccgctg gacgcctcta ccgttcatcc ggaagcctat ccggtggtgg
    84481 aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac
    84541 tgcgtaacct gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg
    84601 acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc
    84661 agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg
    84721 cagtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc
    84781 tggttcacat ctcttcatta tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag
    84841 cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc
    84901 tgactatgcg tctggacgag cagcctggcg aaaccaactc tcgtcgcggc ggcggtaatg
    84961 aacgcccgca aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg
    85021 gtaatagcgc gatgatggat gcgctggcgg cagcgatggg taaaaaacgt taaacgcccg
    85081 gactggcccc taaacctgcg cgataaggcg ttcacgccgc atctggcaac gaacgctttg
    85141 cctgacataa aagtgccgga gaatatctcc ggcactttta ttccacagcc aaactcacaa
    85201 tatactccgt taatatttat cacttcatta acaactgaaa ccttaattaa acattagcca
    85261 gtccgggtaa ctcactattc gaattatatt ttcgctgcga tataaccttg agccacatca
    85321 acattgagtc agattattat tcaaaccaac attcgcacac attttaagta ttgctgatag
    85381 aaaccattct cattatcatt gtgttgttga ttatttaatc tctcctttgt tggcaaatca
    85441 tctggtctca tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa
    85501 gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga
    85561 aatcagcccg gcatatcgcc aaaaactgct ttctctaggc atgttacctg gctcctcttt
    85621 tgatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag
    85681 cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata
    85741 cggtgataac aacaatgaaa aaattaacca ttggcttaat tggtaatcca aattctggca
    85801 agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg
    85861 ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg
    85921 acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa
    85981 tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt
    86041 ctaaccttga gcgtaacctg tacctgacgc tgcaactgct ggaactcggc attccctgca
    86101 tcgtggcgct gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg
    86161 ctctatcggc acgtcttggc tgcccggtga tcccgctggt ttcaacccgt ggtcgcggta
    86221 ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc
    86281 attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca
    86341 tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc
    86401 gcgcctacgc cggtgaagcg tcgcagcatc tggatgccac cctcgcccgt ctgcgtaatg
    86461 aaatggatga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct
    86521 gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata
    86581 aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt
    86641 tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttaacgtc ggctccgtgg
    86701 cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga
    86761 ctatcttcct cgcccagggc ctgggtggcg gtattaacac cgtgctgcca ctggtgccgc
    86821 agatcggcat gatgtacctg ttcctctcct tccttgagga ctccggctat atggcgcgtg
    86881 cagcgtttgt gatggaccgc ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc
    86941 cgctgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt acgcttgatg
    87001 caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc
    87061 tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcc ctggcggttt
    87121 tctcgctgta tatgctcggt atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca
    87181 ccatcatgcg cggtgaagcg acgccgtttg tcatggagct gccggtctac catgtaccac
    87241 acgttaaaag cctgattatc cagacctggc agcgtctgaa gggcttcgtt ctgcgtgctg
    87301 gtaaagtgat tatcatcgtc agcattttcc tgagcgcttt caacagcttc tcgttgagcg
    87361 ggaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg gtgataaccc
    87421 ctgtcttcaa gccgattggc gtacatgaag ataactggca ggcaacggtt ggcctgttta
    87481 cgggcgcaat ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac accgcagaaa
    87541 atattcagga cgaagagttc aatccggcgg aattcaacct cggtgaagag ctgttcagtg
    87601 cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc
    87661 ccattgaagc tagcaaaggc aacggtgaaa tgggtaccgg ggcgatgggc gtgatggatc
    87721 agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg ctgtatgtac
    87781 catgtatctc ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct
    87841 ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat caggtcgcca
    87901 gctacagtca gcacccaact tacagcctgg tgtgcattct ggcggtaatc ctgtttaaca
    87961 tcgtagctat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa
    88021 cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc
    88081 ttcacttatt caggtacgtg atttgctggc gttacggggc cgtatggaag cgacccagat
    88141 aagccagaca ttgaacactc cgcagccaat gattaacgcc atgctgcaac agctggaaag
    88201 catgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa
    88261 aagctgcccg gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc
    88321 catcgcctgt tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt
    88381 tccataaaaa tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt ctcgcaggga
    88441 tgaaaactcg ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca
    88501 ggtgaaggaa cgccatgagt aaaaagcaga gttccacccc acatgatgcg ctgttcaaac
    88561 tctttttacg ccaaccggag acggcacgcg attttcttgc gtttcattta ccggcaccca
    88621 ttcacgcgct ctgtgatatg aaaaccctca agctggagtc gaggagcttt attgatgacg
    88681 atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat
    88741 acatctattg tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga
    88801 tgcgttacgc tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga
    88861 tggtggtgcc attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt
    88921 ggctggattg tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc
    88981 tgattgatgt caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc
    89041 tggagttaat tcaaaaacat attcgtcaac gcgatctgat ggggctggta gagcaaatgg
    89101 cctgcttatt aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca
    89161 tactgcaaac tggcgacgct gtacgtttta acgattttat cgacggcgtt gccgaacgtt
    89221 caccgaaaca caaggagagt ttaatgacta ttgcggaaag attgcggcag gagggggaac
    89281 aatccaaagc cctgcatata gccaaaataa tgcttgaatc cggagtccct cttgcagaca
    89341 tcatgcgatt taccgggctg tcagaagaag agttggctgc ggcgagtaga ctggccccct
    89401 gaatctccag acaaccagta t
//
LOCUS       DAEEDS010000002        84294 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEEDS010000002 DAEEDS010000000
VERSION     DAEEDS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84294
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.002"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            311..1744
                     /gene="cscA"
                     /locus_tag="KLH32_000105"
     CDS             311..1744
                     /gene="cscA"
                     /locus_tag="KLH32_000105"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBE0137114.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1752..2810)
                     /locus_tag="KLH32_000106"
     CDS             complement(1752..2810)
                     /locus_tag="KLH32_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0137115.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2978..3196
                     /locus_tag="KLH32_000107"
                     /pseudo
     CDS             2978..3196
                     /locus_tag="KLH32_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3214..4542
                     /gene="dsdA"
                     /locus_tag="KLH32_000108"
     CDS             3214..4542
                     /gene="dsdA"
                     /locus_tag="KLH32_000108"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBE0137116.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KLH32_000109"
     CDS             complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KLH32_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBE0137117.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KLH32_000110"
     CDS             complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KLH32_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBE0137118.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7767..8381
                     /gene="evgA"
                     /locus_tag="KLH32_000111"
     CDS             7767..8381
                     /gene="evgA"
                     /locus_tag="KLH32_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBE0137119.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8386..11979
                     /gene="evgS"
                     /locus_tag="KLH32_000112"
     CDS             8386..11979
                     /gene="evgS"
                     /locus_tag="KLH32_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBE0137120.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KLH32_000113"
     CDS             complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KLH32_000113"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBE0137121.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KLH32_000114"
     CDS             complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KLH32_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBE0137122.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KLH32_000115"
     CDS             complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KLH32_000115"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBE0137123.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KLH32_000116"
     CDS             complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KLH32_000116"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBE0137124.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17779..18402)
                     /locus_tag="KLH32_000117"
     CDS             complement(17779..18402)
                     /locus_tag="KLH32_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBE0137125.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18699..18974
                     /locus_tag="KLH32_000118"
     CDS             18699..18974
                     /locus_tag="KLH32_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137126.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(19051..19293)
                     /locus_tag="KLH32_000119"
     CDS             complement(19051..19293)
                     /locus_tag="KLH32_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBE0137127.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19646..20566
                     /gene="lpxP"
                     /locus_tag="KLH32_000120"
     CDS             19646..20566
                     /gene="lpxP"
                     /locus_tag="KLH32_000120"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBE0137128.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20921..20992
                     /gene="ypdK"
                     /locus_tag="KLH32_000121"
     CDS             20921..20992
                     /gene="ypdK"
                     /locus_tag="KLH32_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBE0137129.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(21057..22295)
                     /gene="alaC"
                     /locus_tag="KLH32_000122"
     CDS             complement(21057..22295)
                     /gene="alaC"
                     /locus_tag="KLH32_000122"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBE0137130.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22672..24158
                     /locus_tag="KLH32_000123"
                     /pseudo
     CDS             22672..24158
                     /locus_tag="KLH32_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24173..24907
                     /gene="ypdB"
                     /locus_tag="KLH32_000124"
     CDS             24173..24907
                     /gene="ypdB"
                     /locus_tag="KLH32_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBE0137131.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24920..25777
                     /locus_tag="KLH32_000125"
     CDS             24920..25777
                     /locus_tag="KLH32_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0137132.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25780..28011)
                     /gene="ptsP"
                     /locus_tag="KLH32_000126"
     CDS             complement(25780..28011)
                     /gene="ptsP"
                     /locus_tag="KLH32_000126"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0137133.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETE
                     LTF"
     gene            complement(28036..29073)
                     /gene="ypdE"
                     /locus_tag="KLH32_000127"
     CDS             complement(28036..29073)
                     /gene="ypdE"
                     /locus_tag="KLH32_000127"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBE0137134.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29073..30156)
                     /gene="ypdF"
                     /locus_tag="KLH32_000128"
                     /pseudo
     CDS             complement(29073..30156)
                     /gene="ypdF"
                     /locus_tag="KLH32_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30171..31418)
                     /locus_tag="KLH32_000129"
     CDS             complement(30171..31418)
                     /locus_tag="KLH32_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBE0137135.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31440..31766)
                     /locus_tag="KLH32_000130"
     CDS             complement(31440..31766)
                     /locus_tag="KLH32_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBE0137136.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31985..32950)
                     /gene="glk"
                     /locus_tag="KLH32_000131"
     CDS             complement(31985..32950)
                     /gene="glk"
                     /locus_tag="KLH32_000131"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBE0137137.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33154..34410
                     /locus_tag="KLH32_000132"
     CDS             33154..34410
                     /locus_tag="KLH32_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBE0137138.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34525..34851
                     /locus_tag="KLH32_000133"
     CDS             34525..34851
                     /locus_tag="KLH32_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE0137139.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34992..36230)
                     /locus_tag="KLH32_000134"
     CDS             complement(34992..36230)
                     /locus_tag="KLH32_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBE0137140.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36566..37768
                     /gene="nupC"
                     /locus_tag="KLH32_000135"
     CDS             36566..37768
                     /gene="nupC"
                     /locus_tag="KLH32_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBE0137141.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37818..39930)
                     /locus_tag="KLH32_000136"
                     /pseudo
     CDS             complement(37818..39930)
                     /locus_tag="KLH32_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40138..40213)
                     /locus_tag="KLH32_000137"
     tRNA            complement(40138..40213)
                     /locus_tag="KLH32_000137"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40178..40180),aa:Ala,seq:ggc)
     gene            complement(40253..40328)
                     /locus_tag="KLH32_000138"
     tRNA            complement(40253..40328)
                     /locus_tag="KLH32_000138"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40293..40295),aa:Ala,seq:ggc)
     gene            40549..40908
                     /locus_tag="KLH32_000139"
     CDS             40549..40908
                     /locus_tag="KLH32_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE0137142.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40910..41302
                     /locus_tag="KLH32_000140"
     CDS             40910..41302
                     /locus_tag="KLH32_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE0137143.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41354..42769)
                     /gene="gltX"
                     /locus_tag="KLH32_000141"
     CDS             complement(41354..42769)
                     /gene="gltX"
                     /locus_tag="KLH32_000141"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBE0137144.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43028..43103
                     /locus_tag="KLH32_000142"
     tRNA            43028..43103
                     /locus_tag="KLH32_000142"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43061..43063,aa:Val,seq:tac)
     gene            43148..43223
                     /locus_tag="KLH32_000143"
     tRNA            43148..43223
                     /locus_tag="KLH32_000143"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43181..43183,aa:Val,seq:tac)
     gene            43228..43303
                     /locus_tag="KLH32_000144"
     tRNA            43228..43303
                     /locus_tag="KLH32_000144"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43261..43263,aa:Lys,seq:ttt)
     gene            43334..43459
                     /locus_tag="KLH32_000145"
     ncRNA           43334..43459
                     /ncRNA_class="other"
                     /locus_tag="KLH32_000145"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43735..44076
                     /locus_tag="KLH32_000146"
     CDS             43735..44076
                     /locus_tag="KLH32_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBE0137145.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44067..44993)
                     /locus_tag="KLH32_000147"
     CDS             complement(44067..44993)
                     /locus_tag="KLH32_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0137146.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45083..46081
                     /locus_tag="KLH32_000148"
     CDS             45083..46081
                     /locus_tag="KLH32_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBE0137147.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46078..46296)
                     /locus_tag="KLH32_000149"
     CDS             complement(46078..46296)
                     /locus_tag="KLH32_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBE0137148.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46298..48313)
                     /gene="ligA"
                     /locus_tag="KLH32_000150"
     CDS             complement(46298..48313)
                     /gene="ligA"
                     /locus_tag="KLH32_000150"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBE0137149.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48384..49370)
                     /gene="zipA"
                     /locus_tag="KLH32_000151"
     CDS             complement(48384..49370)
                     /gene="zipA"
                     /locus_tag="KLH32_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBE0137150.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49600..50361
                     /gene="cysZ"
                     /locus_tag="KLH32_000152"
     CDS             49600..50361
                     /gene="cysZ"
                     /locus_tag="KLH32_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBE0137151.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50546..51517
                     /gene="cysK"
                     /locus_tag="KLH32_000153"
     CDS             50546..51517
                     /gene="cysK"
                     /locus_tag="KLH32_000153"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBE0137152.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51901..52158
                     /gene="ptsH"
                     /locus_tag="KLH32_000154"
     CDS             51901..52158
                     /gene="ptsH"
                     /locus_tag="KLH32_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBE0137153.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52203..53930
                     /gene="ptsI"
                     /locus_tag="KLH32_000155"
     CDS             52203..53930
                     /gene="ptsI"
                     /locus_tag="KLH32_000155"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBE0137154.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53971..54480
                     /gene="crr"
                     /locus_tag="KLH32_000156"
     CDS             53971..54480
                     /gene="crr"
                     /locus_tag="KLH32_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE0137155.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54522..55373)
                     /gene="pdxK"
                     /locus_tag="KLH32_000157"
                     /gene_synonym="thiJ"
     CDS             complement(54522..55373)
                     /gene="pdxK"
                     /locus_tag="KLH32_000157"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBE0137156.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55478..55852
                     /locus_tag="KLH32_000158"
     CDS             55478..55852
                     /locus_tag="KLH32_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBE0137157.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55885..56608
                     /locus_tag="KLH32_000159"
                     /pseudo
     CDS             55885..56608
                     /locus_tag="KLH32_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56812..57723)
                     /gene="cysM"
                     /locus_tag="KLH32_000160"
     CDS             complement(56812..57723)
                     /gene="cysM"
                     /locus_tag="KLH32_000160"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBE0137158.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57857..58954)
                     /gene="cysA"
                     /locus_tag="KLH32_000161"
     CDS             complement(57857..58954)
                     /gene="cysA"
                     /locus_tag="KLH32_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBE0137159.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58944..59819)
                     /gene="cysW"
                     /locus_tag="KLH32_000162"
     CDS             complement(58944..59819)
                     /gene="cysW"
                     /locus_tag="KLH32_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBE0137160.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59819..60652)
                     /gene="cysT"
                     /locus_tag="KLH32_000163"
     CDS             complement(59819..60652)
                     /gene="cysT"
                     /locus_tag="KLH32_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBE0137161.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDS
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60652..61668)
                     /gene="cysP"
                     /locus_tag="KLH32_000164"
     CDS             complement(60652..61668)
                     /gene="cysP"
                     /locus_tag="KLH32_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBE0137162.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61972..62763)
                     /gene="ucpA"
                     /locus_tag="KLH32_000165"
     CDS             complement(61972..62763)
                     /gene="ucpA"
                     /locus_tag="KLH32_000165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBE0137163.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62950..63750)
                     /gene="murR"
                     /locus_tag="KLH32_000166"
     CDS             complement(62950..63750)
                     /gene="murR"
                     /locus_tag="KLH32_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBE0137164.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63914..64810
                     /gene="murQ"
                     /locus_tag="KLH32_000167"
     CDS             63914..64810
                     /gene="murQ"
                     /locus_tag="KLH32_000167"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBE0137165.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64814..66238
                     /gene="murP"
                     /locus_tag="KLH32_000168"
     CDS             64814..66238
                     /gene="murP"
                     /locus_tag="KLH32_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBE0137166.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66418..67317)
                     /gene="yfeX"
                     /locus_tag="KLH32_000169"
     CDS             complement(66418..67317)
                     /gene="yfeX"
                     /locus_tag="KLH32_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBE0137167.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67413..67988)
                     /locus_tag="KLH32_000170"
     CDS             complement(67413..67988)
                     /locus_tag="KLH32_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBE0137168.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68049..68498)
                     /locus_tag="KLH32_000171"
     CDS             complement(68049..68498)
                     /locus_tag="KLH32_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBE0137169.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68485..68910)
                     /locus_tag="KLH32_000172"
     CDS             complement(68485..68910)
                     /locus_tag="KLH32_000172"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBE0137170.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69124..69993
                     /gene="amiA"
                     /locus_tag="KLH32_000173"
     CDS             69124..69993
                     /gene="amiA"
                     /locus_tag="KLH32_000173"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBE0137171.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69997..70896
                     /gene="hemF"
                     /locus_tag="KLH32_000174"
     CDS             69997..70896
                     /gene="hemF"
                     /locus_tag="KLH32_000174"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBE0137172.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70902..71954)
                     /gene="eutR"
                     /locus_tag="KLH32_000175"
                     /pseudo
     CDS             complement(70902..71954)
                     /gene="eutR"
                     /locus_tag="KLH32_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72000..72500)
                     /gene="eutK"
                     /locus_tag="KLH32_000176"
     CDS             complement(72000..72500)
                     /gene="eutK"
                     /locus_tag="KLH32_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBE0137173.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72513..73172)
                     /gene="eutL"
                     /locus_tag="KLH32_000177"
     CDS             complement(72513..73172)
                     /gene="eutL"
                     /locus_tag="KLH32_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBE0137174.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74354..75145
                     /locus_tag="KLH32_000178"
     CDS             74354..75145
                     /locus_tag="KLH32_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137175.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75865..76260
                     /locus_tag="KLH32_000179"
     CDS             75865..76260
                     /locus_tag="KLH32_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0137176.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76278..76541
                     /locus_tag="KLH32_000180"
     CDS             76278..76541
                     /locus_tag="KLH32_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137177.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76564..77106
                     /locus_tag="KLH32_000181"
     CDS             76564..77106
                     /locus_tag="KLH32_000181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137178.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77245..77532
                     /locus_tag="KLH32_000182"
     CDS             77245..77532
                     /locus_tag="KLH32_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137179.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77571..77777
                     /locus_tag="KLH32_000183"
     CDS             77571..77777
                     /locus_tag="KLH32_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137180.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78089..78574
                     /locus_tag="KLH32_000184"
     CDS             78089..78574
                     /locus_tag="KLH32_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0137181.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78555..78833
                     /locus_tag="KLH32_000185"
     CDS             78555..78833
                     /locus_tag="KLH32_000185"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137182.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78826..80286
                     /locus_tag="KLH32_000186"
     CDS             78826..80286
                     /locus_tag="KLH32_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0137183.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80320..81129
                     /gene="murI"
                     /locus_tag="KLH32_000187"
     CDS             80320..81129
                     /gene="murI"
                     /locus_tag="KLH32_000187"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE0137184.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81248..81942)
                     /locus_tag="KLH32_000188"
     CDS             complement(join(81248..81696,81696..81942))
                     /locus_tag="KLH32_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0137185.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82461..83834)
                     /gene="eutA"
                     /locus_tag="KLH32_000189"
     CDS             complement(82461..83834)
                     /gene="eutA"
                     /locus_tag="KLH32_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBE0137186.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83809..84288)
                     /gene="eutP"
                     /locus_tag="KLH32_000190"
     CDS             complement(83809..84288)
                     /gene="eutP"
                     /locus_tag="KLH32_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBE0137187.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        21902 a        21329 c        20003 g        21060 t
ORIGIN      
        1 aatccggaaa caagtgagcg tttccggatt cttacacagc cactggatcg gtcaactgat
       61 ccttaactga tcggcattaa tactttggct gacatttttt tcccttaaat tcatctgagt
      121 tacgcatagt gataaacctc tttctcgcaa aatcgtcatg gatttactaa aacatgcata
      181 ttcgatcaca aaacgccata gttaacgtta acatttgcga tattcatcgc atttatgaaa
      241 gtaagggact atatttttat aaaagttaac gttaataatt caccaaattt gcttaaccag
      301 gatgattaaa atgacgcaat ctcgattgca tgcggcgcaa aacgcactag caaaacttca
      361 tgagcaccgg ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccggatggat
      421 gaacgatcca aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca
      481 cccgatgagc gaacactggg gaccaatgca ctggggacat gccaccagtg acgatatgat
      541 ccactggcag catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg
      601 tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt
      661 ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag
      721 tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat
      781 gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc
      841 gaaagatcca ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg
      901 ggccttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc
      961 ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc
     1021 cgagggatac agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc
     1081 aggacgactt tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta
     1141 tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat
     1201 gtgggaatcg ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg
     1261 cgagctatca gagagcaatg gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt
     1321 acgccagcag catcaatctg tctctccccg cagaatcagc aataaatatg ttttgcagga
     1381 aaacgcgcaa gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca
     1441 ttacggatta cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact
     1501 tattttgtgg cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc
     1561 gcagcgtgac acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat
     1621 taacgacggg gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact
     1681 gtcgctttat gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg
     1741 ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt
     1801 cccccttcac gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga
     1861 agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag
     1921 cccattacag cgacatcctg cgggatacgt acccccttcg ccaaaagaac ctgataagcc
     1981 acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg
     2041 aagtgagcat tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga
     2101 aattgtttca cctcagccag atctcgtcca gcatcacgcc aagcctgctc aaatccctgc
     2161 cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataatgg ttgacgatag
     2221 cccgccgcga gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa
     2281 ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga
     2341 gactcaggca gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg
     2401 tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa
     2461 aaactattcc agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag
     2521 aaaggagtgg tagccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga
     2581 gcgcgcatct tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat
     2641 acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc
     2701 atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt
     2761 gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa cgccctatca
     2821 cctgacacag caatacaata aaaaataaca ataattcccg gacaattgtc cccaattccg
     2881 cctctgttct cgccaacgag taaccatata ggcaagtaag taaaacatgc caactggcat
     2941 cgccaacctg gagaagaaaa tataaatatg gatcattgtg ctgccactgt atcccgacat
     3001 cagcccggaa attattgcga ttgctatcgg ttcaggtgca attggctgca cgatcgttac
     3061 ggactcgctt ttctggctgg tgaagcaata ttgcggcgct acgctcaatg aaacatttaa
     3121 atactatacg acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct
     3181 gtcatttatc atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc
     3241 atcgcccagt atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat
     3301 cctagcacga cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt
     3361 caggacgccc atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttgctgaa
     3421 acagctgcca ctggggggat tattgaatca gaactggttg ccattccggc tatgcaaaaa
     3481 cggctggaaa aggaatatca acaaccgatc agcgggcaac tcctactgaa aaaagatagc
     3541 catttgccca tttccggctc tataaaagca cgcggcggga tttatgaagt cctggcacat
     3601 gcagaaaaac tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg
     3661 ctttctccgg agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga
     3721 aatctggggt tatcaatcgg cattatgagc gcccgcattg gctttaaggt gacagttcat
     3781 atgtctgctg atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc
     3841 gtggaatatg agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct
     3901 gacccgaact gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc
     3961 gttgctggcc agcgtcttaa agcgcaattt gcccagcaag gccgtatcgt cgatgctgat
     4021 aaccctctgt ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc
     4081 gggcttaaac tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc
     4141 ccttgtatgt tgttaggcgt ccatacagga ttacacgatc agatttctgt tcaggatatt
     4201 ggtatcgaca accttaccgc agcggatggc cttgcagttg gtcgcgcatc atgctttgtc
     4261 gggcgggcaa tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat
     4321 gacatgcttg gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg
     4381 ggtatggccg gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc
     4441 agcgcagaac aattgcgtaa tgccactcat ctggtgtggg cgacgggagg tggaatggtg
     4501 ccggaagaag agatgaatca atatctggca aaaggccgtt aataacgttt caacgcaaca
     4561 tcgcaatcct ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccggtc
     4621 ttacccgcta taaccccctc cttttatcat cacccaacgc ctttcgctgt aaacggcggt
     4681 ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc
     4741 tcatttgcag aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact
     4801 cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta
     4861 aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcgtcg tccccacagc
     4921 gtcattgtca acgacgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc
     4981 gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa
     5041 aagaaacagg caacggcgaa tccctggaaa aactgtggca aaatgatgcc tgtaaaatca
     5101 atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaactg
     5161 aatgtcacta acacccgcat gtctatttta ttgccataac gtcctatcaa aggtgaaatc
     5221 aatagtggca tgatgccgat gggcgcataa gcaagcccgg cccatatcgc attatacccc
     5281 atcgttttct ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg
     5341 caggtgatac tcacaatacc aatggtgaaa ttacgggact taaacaaact gagatcaaga
     5401 atcgggttct ctgaggtcga ttcccaaatg actaaagaga tcagagaaat aactgatact
     5461 actgttaata ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc
     5521 ataatttgca agccaccaac accgagcact aacagggtca gtcttggtag attcattttt
     5581 accggtgaag tttcagtttc tcttccttta agtaaggtta agcataatgt caggacgata
     5641 atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc gcaaatatag
     5701 ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga
     5761 gcaaatgttc ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt
     5821 aacggccccg ccattaatcc ctgaacgact ctaaaaaata tcagcacatc aagattggtc
     5881 gataaactac acattaagga agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt
     5941 aattcgccta ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg
     6001 ccaaacgagg tgataaccca ggtgccttcg tctgttgatg ctcccagaaa gccagatatt
     6061 gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac
     6121 aatgcaatag taacgcacca taacgtccca ccggttaatg gtgccggagt tgatttagtg
     6181 attgccatta aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac
     6241 tggtatcgat aactaaagcc ttactggtat aagcaggcat ggaggtcacg gttgaagcca
     6301 gatcaggcat ctcagcaatg tcttcgtcct tcgtatcaat agttgcagtc atcgataaac
     6361 caatgcgcaa ggggtgttcc atgagttctt ttggatcaag agaaacttca accggtacac
     6421 gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat
     6481 tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa
     6541 gatcgctgat aatattgact gattgaccaa tccgtacatc cgtgagttgt gtttctttaa
     6601 agttggcatt aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccggaac
     6661 tcactgtttc gccgacctga acacttcgct gggcaatata gccagtaacc ggactcctta
     6721 tatccgtacg tttaagcacc aaccaggctt ctttggttgc atccgccgct tcaactactt
     6781 gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga
     6841 tagcggcatt cagtgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt
     6901 cttttgaaat aaccccctgc ttcgctaaag gcacacgacg gttataatct tctaaagatt
     6961 gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtaca
     7021 gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag
     7081 tggcatcagt tttatccagt gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt
     7141 tatgattaac tacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg
     7201 tgacataggc gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg
     7261 cacctgaaaa cgcaataaat aaaactatca ccaataaaga aaagtatttt tttctgttag
     7321 actgtttttt atttgaatta atctgttcca ctattatctc tcatttcgca tagatgaaat
     7381 ttatgaattg aatgaaaaga gtattcattc ttcatatcaa acacagtgca tgtgttgcac
     7441 attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac
     7501 taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtgaga agacttatag
     7561 tgctaacttg aaactataaa tcatcggtat aatccctgat tttattgttg acatttcatt
     7621 tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaaact cagattttct
     7681 tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg gagaagggag
     7741 atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac catcctcttg
     7801 ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta gcggagttaa
     7861 ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa gcctgatata gtcatcattg
     7921 atgtcgatat ccccggagtt aacggtatcc aagtgttaga aacgctgagg aagcgccaat
     7981 atagcggaat tattattatc gtctccgcta aaaatgacca tttttacggg aaacattgtg
     8041 ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat atcattgcgg
     8101 ctattgaagc tgcaaaaaat ggctactgct atttcccctt ctctctcaac cggtttgttg
     8161 gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa attagtgtca
     8221 tgcggtatat tcttgatggc aaggataata atgacattgc ggaaaaaatg ttcatcagca
     8281 acaaaactgt cagcacgtat aaaagtcgcc tgatggaaaa attagaatgt aaatcactga
     8341 tggatcttta cacattcgcg caacgtaaca aaatcggcta atcacatgaa gtttttaccc
     8401 tatttttttc ttctctgttg tggtctttgg tcgaccataa gtttcgcaga cgaagattac
     8461 atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccagt acgtctgagc
     8521 aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc
     8581 caaacggcta ccttgttgca taccgattcg cagcaacagg ttcgtggtat taatgctgat
     8641 tatttaaatc ttttaaaaag agcgttaaat atcaaattaa cactccggga atacgcagat
     8701 catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt atcacattta
     8761 gttacttcgc cgcctcttaa taatgacatt gctgcaacca aaccattgat aattaccttt
     8821 ccggcgctgg taaccaccct tcacgactca atgcgaccgc ttacctcacc aaaaccagta
     8881 aatattgctc gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc atttccaaaa
     8941 gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc agctgggcac
     9001 aatgattact ttattggtag taacatcatt accagcagta tgatttcccg ctatttcact
     9061 cactccttaa atgtagtgaa atattataac tcgccgcgtc aatataattt tttcttgacc
     9121 agaaaagaat ctgtcattct taatgaagta ctcaatagat ttgttgatgc tttaacaaat
     9181 gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc ctttctgaac
     9241 aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc caatttaaag
     9301 gtgctggaaa atccttactc gcccccctat tctatgacgg atgaaaatgg ctcggttcgg
     9361 ggcgttatgg gggacattct taatattatt accttgcaaa caggtttaaa tttttctccg
     9421 atcaccgttt cacacaatat ccatgctgga acacaactta gccccggagg atgggatata
     9481 atacctggcg ctatttatag tgaagatcga gaaaataatg ttttatttgc tgaagccttc
     9541 ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa
     9601 aaaggaatga aagttgccat tccatattat tatgagctgc attcgcaatt aaaagagatg
     9661 tatccggagg ttgaatggat acaggtcgat aatgccagcg ctgcatttca caaggttaag
     9721 gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat
     9781 tactatccta atgaacttta tcattttctt attcctggcg ttccgaatgc atcgctttcg
     9841 ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc actgaatgca
     9901 attcccccaa gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc
     9961 attgacacat gggacctata tagcgagcaa ttttatattg ttacgacatt atccgtttta
    10021 ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg tcgtcgtaaa
    10081 gtcattcagg gtgatttaga aaaccaaata tcattccgaa aagcactctc ggattcctta
    10141 ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcataa tagtgctttt
    10201 gaacattatt tcactgcgga ttactacaaa aatgcaatgt taccattaga aaacagtgat
    10261 tcacccttta aagatgtttt ttctaatgcg catgaagtca cagcagaaac gaaagaaaat
    10321 cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag atgcattaat
    10381 cactggcata cattatgcaa tcttcctgca agtgacaatg cagtatatat ttgtggttgg
    10441 caagatatta ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa aaataaagcg
    10501 ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga aataagaaca
    10561 ccaataagct ctattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggag
    10621 caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt
    10681 ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg
    10741 gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc
    10801 aaatcgatcg cattaagttg cagcagtaca tttcctgatc attacctggt taagatcgac
    10861 cctcaggcgt ttaagcaggt cttatcaaat ttactgagta atgctctcaa atttaccacc
    10921 gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca cgctgttatc
    10981 aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactgttt
    11041 aagcgctaca gccaaacaag tgcaggtcgt cagcaaacag gttctggttt aggcttaatg
    11101 atctgcaaag aattaattaa aaatatgcag ggcgatttat cattagaaag tcatccaggc
    11161 ataggaacaa catttacgat cacaatcccg gtagaaatta gccagcaagt ggcgactgtc
    11221 gaggcaaaag cagaacaacc catcacacta cctgaaaagt tgagcatatt aatcgcggat
    11281 gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt
    11341 gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg
    11401 cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg caaactccgt
    11461 gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt
    11521 gaaaaagggt taagttgcgg catgaactta tgtttgttca aaccgttgac cctggatgta
    11581 ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac
    11641 cttgatatcg aagccctgaa aaataatacg gcgaatgatc tacaactgat gcaggagatt
    11701 ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca tgcactagaa
    11761 gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc
    11821 ctgaatttgc aaaagttgat taatattagc catcagttag aaataacacc tgcttcagat
    11881 gacagtaagc ctgaaattct tcagctgctg aactctgtta aagaacacat tgcagagctg
    11941 gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc
    12001 taatgtggga gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt
    12061 gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc
    12121 tgatttttat cggattaccc ggcatcatta ttcctccggc ttcaatcaac atatttctcg
    12181 cctgagtttg tggcaaatta atggcctcag ccacacttaa taacggcgcg acgggcacac
    12241 caacttcatg tattttggct aaccaaactt cagctgcctg cgtttttaac gttcgctcaa
    12301 tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatcggggat
    12361 cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac
    12421 cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg
    12481 gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc atcagaccat
    12541 gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctctggc
    12601 tcttttcgcg gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata
    12661 gatccgcaag agaggtacca acacgcactg gcggagcatc agggtaccct gtttccatca
    12721 ttatcccact cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg
    12781 tatgtccgaa acctgacgat gaagcatata tgaggcgcgg gttgatttct tgtaaccttt
    12841 cccatgaaaa ccccagtttt tccattgtac ctaggcgaaa attctcagct aatacatcag
    12901 cttgtttgag catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca
    12961 cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac
    13021 caaatgtgcg ggtatcatca ccatgacctg gtggctcaac tttaattacc cttgcgccca
    13081 tattataaag aagttgagtt ccgaaaggtc cattaaggac atgtgtcata tcgataacta
    13141 ataagccttc aaacggccct ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa
    13201 gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat taattatgaa
    13261 accagtcgac tgacataaat ccacaaggga gccgtgacga caaagcccag tacgctcaca
    13321 gccaatgacg ctgtaccagt acgggtgtag acattaaacc ggctggcaat aatgattccg
    13381 gagaatgccg gcggtaatgc gcctgccagt accatcatct gcaaatgttc gctgttcaaa
    13441 tgacatgcca taccaacgag aagcagcgcc agtggcatca gaatcaactt caggaaggtg
    13501 ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg
    13561 aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc ccatgctgcc
    13621 ggaattttta ccccaaccag caccaggatc gttgccagaa caggtgccca tactactggc
    13681 tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc cgctcctgag
    13741 gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat aatagaaata
    13801 attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag aactgcaaac
    13861 cccaggaatc caatggtagg tgaacccgca attaatgcac atacagctgc ttctgcatgg
    13921 gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa agaaacatcc gacaataacc
    13981 acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta
    14041 atagaaacaa atagagccgc aggaagagcg tagtttaata ccagtttatt aaatgcgcgg
    14101 gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag catgattacg
    14161 ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc gataagggtc
    14221 ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta attaccagcg
    14281 acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc agggtcgata
    14341 accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc atgacgaagt
    14401 tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa tttgtcatac
    14461 cttgcatggt gcaacagatc cgttggtgat ggtgcaccag cgccactgag atcaacaccg
    14521 tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag gttatatcga
    14581 caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc aatggcgaca
    14641 accggagaac cagaagtcac gctagcaccg atggcatagc ccataccgat gcccatgaca
    14701 ccccaggtac cacaatccag acgacgacgt ggtttataca tatcaataat atttcgtgca
    14761 ttatccaggg tatttgcacc ttcattaact aaataaatat cctggttctc gcgcaatacg
    14821 tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt acttaatttt
    14881 tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg ccataccagt
    14941 ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact ggatgcaata
    15001 tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc
    15061 agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccaa taaccaattc
    15121 agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga acgcgcagct
    15181 gccgcagaaa gtggatgcgt atcttcaagg atccctttcg ccatagacat tggcaggaat
    15241 ggaatctgag cactttcaat aaattcacga agctgttcat cagcttgtga atacgccgcg
    15301 cctttgccaa ggataattaa tggccgttca gcttttgcta aaagcgaaat tgcgctagtg
    15361 actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac aatcgtggtt
    15421 aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc aagataaact
    15481 ccgccagggc gacccgatac agagacccgg atagcgcgtg ccaatgcaat gccaagatcc
    15541 tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt catttggtcc
    15601 agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc
    15661 atgataatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc attgaggaat
    15721 cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc gcttgccgca
    15781 gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg
    15841 gcatggcgtg ccatatccgt cacaggaata cctacaacac cataaatagt gtcaatatta
    15901 ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag ttgatctgac
    15961 atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt taatatcaga
    16021 tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc tcctgcaata
    16081 cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta aaggcagaga
    16141 atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc acttcaacaa
    16201 cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt aaaaccggtg
    16261 cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca atagtgacag
    16321 tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac
    16381 tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg ttttcccagt
    16441 tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag cctttacatt
    16501 tcaggatcca gccaggctga ccgccaccac ctgcattacc accgcgggga actgcatcac
    16561 caaatgtacc attcggatac tgcgggtatt cttccagata acccaattta tcgagacgct
    16621 gctggtcacg taatttcacg cggcaaaggt tcaatacggc atcctgcatt gacatggtga
    16681 ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca
    16741 aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc ggaccatccc
    16801 aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc aacgttttca taggctttta
    16861 cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc agacgtggat
    16921 tgatttcttg aatatgctcc caggtgaagc ccatgtgatc aatggcccct ggatgaaagt
    16981 tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg ccttccgctg
    17041 ttttggtatt taactcaata gaacgtttgt tactgttaag catggtgaag taaagcgcat
    17101 cgatatcagg aatatctcgc agttggtgac gcgttacgtc accaacgccg ggacgttcaa
    17161 ttttaataac gtcagcgcca aaccaggcca gcatttgagt acaagatggg ccagattgca
    17221 caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca atacctatta
    17281 gttaattaaa tatgtgttaa tttagcgttt atttaaggca taacgccctc tataaataat
    17341 ctgcaaaata tcttcgtagc ggcactcgat atttttaaat tcctgtatca agtactgaaa
    17401 attattttat cgccacttcc aattcctttc tgcatggtaa gtagtgagga cgaaaagaag
    17461 aatatgataa atattgattc gtggtaatca gagagagtcg cgacatttgt gatgttattc
    17521 ttacaactgt aagaagcgcc ggtgatttac aagtaagagt atgattacgc tttttaaagc
    17581 gtaccataaa gcataacata ataaatgatt tgtgatgaca cgggattatt gcggtttact
    17641 ttgaggaaaa taaaatgcgt gtttatagaa ccgcgagcgc caaataattt tatgaccaca
    17701 acctgtcgta ttgtggtcat aaaaaattgt cggctggagg gagttaaatc ctttacctca
    17761 atatactgcc tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga tgattccatc
    17821 ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg
    17881 cgcatcagca agtgcattac gagtctgttt aagcggcatc agatattggt tttccattac
    17941 gccaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt tcgccatttt
    18001 ttcattggca atttttatcg cggcttcttt ttcgggtgtt gctacataac tctcagaaat
    18061 tccgacagat gcattaatga caatatattg gtcatcattg agattggtct ttttacccgg
    18121 cttagcgaac tttgcccatt cggtactgtc atctgacagc aaagcggaag cttcattggt
    18181 cagttctttt gccttttccg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc
    18241 acgcatcgca tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg
    18301 ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag ttgaagatgc
    18361 cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct cattctttaa
    18421 cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat cagagagcaa
    18481 cggtcatttt gaaagggtta tcttacaatt gtagttatta tcgcaaagcg gcatttagtc
    18541 ttacagttgt aggaatatgc ctcctgtcgc agagaactat tcttatatac cacaaagaga
    18601 tgtcatttat tatattgatg taaatattga ttaattatat gtaaatacag ccataacatc
    18661 gtatggttct cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt
    18721 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
    18781 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac
    18841 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgtgccac
    18901 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
    18961 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
    19021 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
    19081 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacca gttcattacc
    19141 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
    19201 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
    19261 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
    19321 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
    19381 gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt
    19441 gcacgattaa taatcagtag ctgaaagcag tcagccagtg ataaagtaga gccaccgcat
    19501 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
    19561 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
    19621 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
    19681 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
    19741 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
    19801 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
    19861 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
    19921 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
    19981 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
    20041 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
    20101 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
    20161 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
    20221 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
    20281 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
    20341 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
    20401 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcttatat
    20461 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
    20521 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
    20581 tctttccctc ctcaaatcgg ccatgataac ctcatgattt ttatagcttt tttcttaagt
    20641 tttttccttc gctgaatatc taattattac cgtcagaaat taaactgtcg ccgatatgag
    20701 acacttgtaa gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg
    20761 cgtacattag cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa
    20821 aatacagggc ttttaaggtc gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa
    20881 gtcattcagg cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg
    20941 catttctttt atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac
    21001 tgtgcaatga aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat
    21061 tccgcgtttt cgtgaatatg tttgctgctg acgggtagta aaccgtcggc acggaacatc
    21121 gctttaatcc ctctgatggc ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga
    21181 acatgcgtat caccatagtc gccaaagcca atccccggcg agacacaaac cttcgcttcg
    21241 ttaaggagct tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc
    21301 gcccagacat acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct
    21361 ttaaccaata catcacgacg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga
    21421 tcgccctcga gtgccgcaat cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga
    21481 tagcttttga ttcgcgccag ggcactgacc agcgttttgt tgccgaccat aaagccgata
    21541 cgccagcccg ccatgttgta gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt
    21601 gcacccggca cctgcataat tgacggcgct ttccagccat cgtagacgat atcggcatag
    21661 gccaggtcat ggaccaccag cacatcgtag catttcgcca gcgccacaac cttttcgaag
    21721 aactccagtt ccacgcattg cgcggttggg ttcgacggga agccgaggat catcatcttc
    21781 ggtttcggat aactttcacg aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc
    21841 accagcggca ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg
    21901 taacttggat tcggcaccag caccgtgtca ccatgatcca gcgtcgccag catcagatgc
    21961 gccaggccct ctttcgaacc aatagtgacg atggcttctg attccgggtc gatttcaacg
    22021 tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa
    22081 gtggagtaac catgcgtgtc cgggcgctgg gccacagtac ataatttttc gacgatatgc
    22141 ggcggagtcg caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga
    22201 cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg
    22261 cgcgtaaagc gacgttcagg gcgagtgtca gccataattt tctcagatta acgttagcgc
    22321 ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc ctgattaatt
    22381 tctgtctgtc cagcgtttta gaacattttt atcaatggat ttaaatgagg aactccactt
    22441 caaaaacagg aaatttttct acgctaacaa gaaaatcacc accaatttta ttaacattat
    22501 aatttcaata aatttaccag ctagaagtgc attatcaaga gatacattgc taatacacca
    22561 tttatctttc aatccccttt taaatttatg gtttttcctc aaaaacggcc tgctggtcat
    22621 cagccttcag gttacctatc atagaggttt aatccttatt cagagtcacc cgtgcacgaa
    22681 atattcaaca tgctgctggc ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc
    22741 tttctcatcc gtatccgcct gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa
    22801 gaattgctcg ccgttaccgc cattttctcg ctgttcgccc tgttcagcac ctggtccggc
    22861 gttcccgtag aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt
    22921 ctgtttggcc cgtgggtagg catcattacc ggcgtgattg cgggtattca ccggtattta
    22981 attgatatcg gcggcgtgac ggcgatcccc tgctttatca ccagcattct ggcgggttgt
    23041 atatcaggct ggataaacct gaaaatcccc aaagcacagc gctggcgctg gatatcgcca
    23101 acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag gtctgcgaaa
    23161 ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat catgtgctgg
    23221 cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt agcccgacta
    23281 cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca
    23341 caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa
    23401 cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag gaaatggcgg
    23461 tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag cagctacgtg
    23521 aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc cattttctgt
    23581 ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt
    23641 tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac gatgagcaaa
    23701 tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag caggcccgct
    23761 ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc tgcattccca
    23821 gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag ccttgcaaag
    23881 gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc
    23941 gagataccgg gcacggcatc gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc
    24001 cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg
    24061 ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt cctaaccaac
    24121 gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata ttgtgaaagt
    24181 catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa ttaaagagca
    24241 cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga agtttttgca
    24301 gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt
    24361 gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca tcaccgcgtg
    24421 gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc tcaaaccgta
    24481 tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc aacaacagca
    24541 gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta atctggtgaa
    24601 agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag cgcacgagaa
    24661 aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt
    24721 ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt gtgtcaatct
    24781 gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc gactgaaaga
    24841 tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc agttaatgca
    24901 tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac aatttttcgc
    24961 cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct ggtttgccag
    25021 ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc tggatatcgt
    25081 gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg tggaaggaga
    25141 aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac cggtgatgct
    25201 gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata gccgcaccac
    25261 gtcgttgttg catcctgctc gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga
    25321 agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga atatcattca
    25381 gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa
    25441 ttcacaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc aggataatta
    25501 tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc ccaatcatct
    25561 gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc gttgggtgcg
    25621 aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg aagtggcaca
    25681 gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct
    25741 gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta attctgtttc
    25801 cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc ggaagttttt
    25861 attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc cttcgttagc
    25921 acccagcgtc agcatgatat tctccagcgc cagcagtggg cgaacgtctt cttccggcgt
    25981 aaaggcggtg agtaacgctt caatttcctg cgcactgcgg cattcacatg cctgacgcgc
    26041 cagttcccga cacgcctcgc tatccagttg acgaagctgg cttttcaccg ccggaatacg
    26101 cgggctactc atactcagct catccaggcc cagcccaagc agtagcggca gataacggct
    26161 ttcaccgccc agttcaccgc aaatgcctac ccatttgccc cgctgatgcg cagtggtaac
    26221 tatttgctgc aacatgcgca ggaacgatgg cgtaatcggg ttatatagcg gcgatacgcg
    26281 cgggttatta cgatcgaccg catacagata ctgggtcata tcgttggagc cgatactgaa
    26341 gaaatcgacc tcatcgcaga agtggtcgat gatgtagcac accgacggca cttccaccat
    26401 gatcccaagc gtaatcgtct ctgcatgacg caggccatcg cgcttaagct caacgatcgc
    26461 tttttggatc tcgcctttca cccataagat ctgatcgagg ctgtgaacca tcgggatcat
    26521 caactgggcg ttgccgaaac tggcggcgcg caaaatggcc cgcagttgag tgcggaacag
    26581 gccagcaaat tccgggtaaa tacgtaccgc gcgatagccg aggaacgggt tctcttcctg
    26641 gggaatattc agataaggaa tgcttttatc gccgccgatg tccatcgtgc ggaagataat
    26701 cggcttgtcg cccgccgcca gtagcacctg ctggtaggct tcaaattgct cctgctcgtc
    26761 cggcgcgctg tcgcgatcca tatagagcat ttcggtacgg aacagcccga caccttccgc
    26821 gccgttggca aacgcgcctg gcgcttccag agcggtgccg atattcgccg caatatcaat
    26881 acgcttgtta tcacgggaat aggccagctg cgcggcagcc tgcgcctgtt gtttttggcg
    26941 tttatccgcc agcgtctgcg cgacctgata ataaccgctc accgcgtcat tcgggttaat
    27001 cgccagcacg ccgcactggg cgtcaagcac tgcaggttgc ccggcataac gggcaatcgc
    27061 atccagcggc aagccactca gtaccgggat cgccgaggca cgggccagaa tcagtgtatg
    27121 cgaggtgcgc ccggttttct ccagaatcat acccgcaaga tttttcaaat caaggctcaa
    27181 aaactggctt ggggttaaat cttcagccac cagaatggtc ggtttttcca gcaccagatt
    27241 gttgcgcggc ttcagttccg gccaggtgat atgcagcaac tgttcgctga tatcgcgaat
    27301 gtcgctgacc cgctcgcgca gataatcgct ggcagaagca gatagtttgg cgcaaacctg
    27361 ctccatattg ctgatgatcg ccgcccccag tccctgatgc tgttctgtca tcaggcgacg
    27421 gatattgcct gcaaattcat catcctgaat cagcgacaaa tgagcgctga ggatagtttt
    27481 gctttcgccg tcacgctcac gcaattgctg attcagttgc tcggcaagcg ttgccaggct
    27541 gtgctccagc cgggtggaat cttgcgcact ggcggggatt gcccgataac tgtcgaggct
    27601 gtcgctttgt aacagggtca gcgtacccac gccgacgccg cttgccagca cattgccgta
    27661 cagtaaatcc gggttaaggc ggcttaatga acgcggcagc ggatgcgccg tcagttctgc
    27721 ctgcgtcggc tgaacgctgt cgctgtcgat aaagcgcacc aggatgtact cttccagcac
    27781 gcgccgcgcc tgctcttcat cgctgccgct aatgttcagg ctgcaactgt cattaaataa
    27841 ggtgccagtt ccaatcaacg ccagcgagct tttagcatca gcctttgcgt tctggcgatg
    27901 gttgataaag gtgatttcgc tttgccactg gctgcactgt tctttaagtt cccacgccgg
    27961 acgagcatgt agaccgttag gcaaaggaca gagaaattga atcgttaaca taaccgctcc
    28021 gggttaatca ggagatcatc tgaaatccgt cagttgaaca accgtctcac gcgtaagacg
    28081 ttgaataagg gcagataaaa gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga
    28141 tgccgcgcaa tgtccgtggc gggtggcagg ccctattacc aatgtgggta cgccagtacc
    28201 ggttaagtgc accgctccac cgtccgtgcc gccgttactg aacatatccg cctgcaacgg
    28261 aacgccaatt tctgccgcca cggtttcaat ccacgcggta agttttggcg gcgcaatcag
    28321 cgatttatcg cttaacacca gcatcggccc gttaccaatc tgacgatggt tagccgcgcc
    28381 ataatcaaag tttttcgccc agcaggcggt atcaagcaca atggcgacgt ccggcgacac
    28441 cgcgcgggta gcagtttgcc cgccgcgtaa tcccacctct tcgctggaac ttgccaccag
    28501 ccacacttcc gcaggcagtt cggcgtcgtg cagctcacgt agtaacgtca ccagcagata
    28561 gcaactgagg cgatcatcaa aggctttgcc catcacccgc tggtgaggga ggacctgaaa
    28621 agtggtatcg aaggtgacgc gatcgcctgg acgaattccc gcctgcatca cttcgtcata
    28681 ggtgcgcgcg ccaatgtcca cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc
    28741 aagcaggcct ggaattttgc actcttcacg ggtggtgatg cgcaccggct gcagctggcg
    28801 ggcagccatg cgcacgttgc caaccggcag cacgtcaata gcgccttcgc gggagatgct
    28861 gcgcaccata aatcccactt cgtccatatg ggcgcagatc atcaccttcg gacctgtcga
    28921 ttcattgagg cggatcagca ccgatcccag accatcaaat cgcacttctt tttgcaggcg
    28981 agccgcttct tccagcagga tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc
    29041 ctcgctcaac gcttttaata gcgataaatc cattatgcct ctcccgtgag caacactgtt
    29101 ttcggcatgg cgtagagcac ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg
    29161 cgcacgcccc cttgccctag caaataaatc cccggctcca cggtcagcaa catgcctggc
    29221 tgtagcgtcg tggtgtcccg cggtgaaaaa cgcggatctt catggacttc aatgccgata
    29281 gcgtgaccag tgttatgacc gaaatagtcg ccaaatccag cctcggtaat gacccggcgc
    29341 gcggcgtcgt caatctgctg gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc
    29401 tgtgcctgca ggacaatctg atagacgtca aacagcgggt gagattcggc gctcacccct
    29461 tcgccattca ccagcaaggt gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa
    29521 atcgagagtg acaaactcgc ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag
    29581 cgccccgcgc cagccgctgg cgacaatggt gtcaaaagag gctttttctg cgccctgctg
    29641 gcgcataaac cactccagtt cagcggctat ctcgcgctcg ctcatccccg cctgaataaa
    29701 gcggcgaata tgctccgctc cgcgatcggc aatcccacag gcgaggcgga ttatctccac
    29761 ctcctctggc gttttgattt gccgcagcac atccggcgtg gcgctaacca gtttcgcatt
    29821 gagttcagac ttccagcggt gcgcggtttc ccaactcacc tgctggcctt caaaaccgag
    29881 cgtttgcaac tgctcatcgg caatgatttg attgacgata gtggtaagcg tgttcgtcgc
    29941 gtcaagcaaa tgcagctggt agccttgcgc acgggcttcc acctcaacga aatagcgcga
    30001 atctaccaga atgtgcgcac tttcacggct aataaccaca tagcctgatc cggtggagat
    30061 ccccagatgc ggctgtttgt tctgccgtga ggagagaagc accgcatcca gttgttgcac
    30121 cttaagccag ttgcgcagcg aagcgagtaa tgtcatctgg attttccttc ttacaggcta
    30181 tcgattaaca atttgccttt gcggaacatc atcagacgca ggaacaccac catcagcgca
    30241 gtaatgattg cccccagcgc gatccccgcc atataaacgc ccaggttagt gaccagcggc
    30301 catgcccaga tagcggattc cgggaaccat tgcactgcgc ccagccagac agctgcggtt
    30361 gagccgacaa tcgcgccgac catatatgac ggaatagcgg tgatggggct ttccagcgca
    30421 aacggaatcg caccctcact aatccccata aaggccagga acatcgcggt cttaccctgc
    30481 ggataaagtt gagcgttgaa caggcgtttg ccggttaaac gacggtcaat aatggtcgcc
    30541 agacccagac caatcggcgg aataacgata gcgatagaac gagcggtgac cggtaatacg
    30601 tggtcagtgg taaagctgaa ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg
    30661 tcgattgctg tcgccgcggc gatccccatt gcgtacatca gcgcgccctt ctcacctgcg
    30721 gcagtcagca cagtacggat accgccgttg atccagccac caaagggagt gatgacgtag
    30781 tacatcgcca gcatgacaaa aatggcggaa agaatcggta gcaaaaatgt ggttttgaac
    30841 gctaacagga aatccggtaa ctggattttc tggttcatcc atttcacaag gtaaccggca
    30901 acgatagaaa taatcagcgc accaatgaag gtggacggca ccggcgaaga ggtcgcccat
    30961 tgcatagtgc tgggatcgaa gttcagcagt tgcgtcggct gggtagacat caacccgccg
    31021 ataaaaccag ccgggaacgc cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac
    31081 atcggaatgg caaaaccaaa caacacgccg ccgaaggact gcgacagcca ggcaaattta
    31141 agtaaggaga ggtcgaaacc ggagaatttc ccgctattaa gagcatccat gatgccgatc
    31201 tcagcaggaa ttttaagcca gctataagca atcagctggc tgaaagcgag gatcacaccg
    31261 cccataatta aggtcggcac catgcgtgaa atccctgaca tcacatgctg cgggagttca
    31321 ccccagaagc tgcttttaga ggcctgcgga ttcttaactg ccgctgccgc accggatgcg
    31381 ccaggcacaa cggttgcact gcgtttttta atggccataa caattatccc tgtcgaatat
    31441 tactgttgtt cagaggcaat catctcttcg atttctttga tgatgcccgc agcgttttta
    31501 attgcgtcct gcaaagtgat ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc
    31561 ggggtaactg ccacggaatg gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg
    31621 cgattctgga tgccgtccgc gccctgggtt tcaattttca cttcataacc ggcttctacc
    31681 gccgcttctt ccagcacctg agcggccata aaagtgtgag ctaggcccat tgggcaggca
    31741 cataaggcaa tcagtttctt actcatcgtt aattcctcgt tcagtgatag ttgacgagtg
    31801 aaattatgaa cgtgcaaaac aaatcgccgt taccagacaa ataatgcatt tactggagaa
    31861 ttaatcccgc tgtacggaag tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa
    31921 ttgaattaac tgtatgattt aaaagattat cgggagagtt acctcccgat ataacaggaa
    31981 ggatttacag aatgtgacct aaggtctggc gtaaatgtgc accggaaccg agaagacccg
    32041 gatagtcatg gacgatgaga tacaccggaa tatcatggac atattcttta aagcgccctt
    32101 tatcttcaaa tgcggcacgg aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga
    32161 taccgcccgc aataaacacg ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa
    32221 aacggcccat aatgacgcaa aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag
    32281 ccagcgcgcg ttcggtaata tcttttggct tgagattttc tggcaggcgg ttgtcagctt
    32341 tcacaattgc gcgatacaaa ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa
    32401 catgaccaat ttccgcacgc aatatttcga ggataatggc ctcttcttca ctattcggcg
    32461 caaaatcaac gtgaccgcct tcgcccggca agcttaccca acgcttatcg acatggacca
    32521 gatgcgcaac cccaagcccc gttccggcac cgtaaaccgc aataggctta ccttcgaccg
    32581 gttctgcgcc accaaactga atcagatgct cttttttcag catcgggatc gccatcgata
    32641 cagcggtaaa atcgttaata atttccagat ggctaaaacc gagattcttt ttcatttcgg
    32701 caattgagaa cgcccaggta tggttggtca ttgccaccca gtcaccggta attgggcaag
    32761 cgatggcaat acagccgtct ttcacctcga ccttatgttc ttcaagataa acgcgaatga
    32821 ccgcttcgag gctggggtaa tcaagccctg aataggtctt agcctgcgag atttcaccac
    32881 tggcaatatc acacagagca agacgtgcgt tggtgccgcc cacatcaccg actaatgcat
    32941 actttgtcat tcttcaactg ctccgctaaa gtcaaaataa ttctttctca cactgtaaat
    33001 acctgggggc ataacaacaa cgccggaaag gcaggctccc tgtaaatatc gatctgggtc
    33061 acacaattac tttatcgttt cagcaccaat tgcagcgatg cctttttgca agctgggcaa
    33121 actaagtatc tgaccccgca taaggaatag aacatgctcc atccgcgagc cagaaccatg
    33181 ttgttattat cgctccccgc cgtggcaatt gggattgcgt ccagtcttat tctgattgtg
    33241 gtgatgaaaa tcgcctcggt attacagaat ttgctctggc aacgactgcc gggaactctg
    33301 gggatagccc aggattcacc cctctggatc atcggtgtat taacgctaac gggtattgcg
    33361 gtggggttgg ttatccgttt cagccagggt catgccggac cagaccccgc ctgtgaaccg
    33421 ctgatcggcg caccggttcc gccctctgcg ctacctggac ttatcgtagc attaattctc
    33481 ggtcttgctg gcggcgtcag cctggggccg gaacatccga tcatgaccgt caatatcgcc
    33541 cttgccgtcg ccattggcgc tcgtctgtta ccgcgcgtca accgaatgga gtggactatt
    33601 ttagcctctg ccggaaccat cggcgcgctg tttggtacac ctgttgcggc ggcgttgata
    33661 ttttcgcaaa ccttaaatgg cagtagtgaa gttccgctat gggatcgtct ttttgcgccg
    33721 ttaatggcgg cagcagctgg tgcacttact accggattat ttttccatcc ccatttttca
    33781 ctgcccattg cgcattacgg acagatggaa atgaccgata ttctcagcgg tgcaattgtc
    33841 gcggcgattg ccatcgcagc agggatggtc gccgtatggt gcttaccacg gttgcacgcg
    33901 atgatgaatc aaatgaaaaa tccggtgctc gtgctgggta ttggcggatt tattctcgga
    33961 attctggggg ttattggtgg accagtttcg ctgtttaaag ggctggatga gatgcagcag
    34021 atggtggcaa atcaggcttt cagcaccagc gattactttt tgctggcggt aattaaactt
    34081 gccgccctgg tcgttgctgc cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg
    34141 tttgtcggcg tggcattagg gttgatgctg catgagcacg ttcccgccgt tccagcggca
    34201 ataaccgttt cctgcgctat tctcggcatc gtgctggtgg taacacgcga tggctggtta
    34261 agtcttttta tggcggcagt cgttgtaccc aataccacat tgctaccgct gctctgtatc
    34321 gtcatgcttc cggcatggct gttattagca ggtaagccga tgatgatggt caatcgtccg
    34381 aagcaacagc caccccacga taatgtttag caaaaaatgc tctcctttat gatttaagag
    34441 ttatggcgat gatacggggc tttacctccc cgtaatattg cgttaacagg ccgctgacgg
    34501 cttcgatcgt gaaggagaat aacaatgttc aggtcactgt ttctggcggc cgccctgatg
    34561 gcatttaccc cgcttgcagc aaatgcaggt gaaatcaccc tactgccatc gatcaaatta
    34621 caaattggcg atcgcgatca ttacggtaat tactgggacg gtggtcactg gcgagatcgt
    34681 gattactggc atcgcaatta tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc
    34741 tatcatcgtg gctgggataa gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc
    34801 gatcgtgacg atcatcgcgg aaaaggccgc ggacatgggc accgccatta aacgcgtcct
    34861 ccaatggagc acaatgcctg atgcgacgct agcgcgtctt atcaggccta caaaccggct
    34921 ctttaacgcg tagggcggat aaggcgttta cgccgcatcc gccaatagtg ccagatgcga
    34981 cgctcattca actacaatcc cagcgccgtc cccaccaaca accagatatt cagcgccacg
    35041 accagcacta caatcaccca gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc
    35101 atcaacttgc tgtcactggt gaaaatcagc agtggaacca gcgccagggc gataccaaaa
    35161 cttaacagca cctgactcat aaccagaatc cgtgtcggat ctaatcccat cagaatgaca
    35221 ataaatgacg gcaacatggt gactgtacga cgcacccaca gcgggatatg gaagcgaatg
    35281 aatccctgca tcaccacctg ccccgccagt gtccccacca ccgttgagga cagtccggca
    35341 gcaaccagac ttaacccaaa gaccgttgcc gcagcatggc ttaacagcgg ttgcagcgtc
    35401 agataagcct catcaagatc ggcaacacca gtatgaccag aaaagtggaa cgccgccgca
    35461 gctgtagcca tcatcaccag attgacaaaa ccggcaatcg tcatggcgat agccacatcc
    35521 catttggtgg cggaataacg ttgttgacgc gaaccgccat gtaaatgctg agtgagcgaa
    35581 gagtgcaaat aaatcacatg cggcataatc gtcgccccta acacgcctgc tgccaggaag
    35641 accgcctccg aagtaggtaa actcgggatc accattcctt tacccagctg cgccaggtta
    35701 ggctgggaga aaatcaactc gacaatgtaa gccgcggcaa caaacaacag taacccgcca
    35761 atcactttct ccagcggttt ttgcccgcga cgttgcagca ttaaaatcag gaaagtcgcg
    35821 atccccgtca gcaccgcgcc ctgcaacaac gaaacaccaa gaatgagttt aaaaccgatc
    35881 gccgcaccaa taaattccgc caggtcggtt gccatcgcaa taatttctgc ctgaacccaa
    35941 tagaaccaca ctacgggacg cggatagtga tcacgaatct gctccgccag atttttaccg
    36001 gtggcaatcc ctagtttggc agagaggatc tgaatcagca tcgccatcag gttggcccaa
    36061 acgacaaccc acagtagctg atagccgaag ctagcacccg cctgaatatt ggtcgcaaag
    36121 ttaccgggat cgatataacc aatcgccgca atgaacgcag gtcccattaa tgcgagcctc
    36181 atcttgcgcg ccgcccgtcc gctgctactc tcaacgcgat agttcgtcat cttgtgcctc
    36241 taaaacatag cctttgctat gtttcatgct atgctaaacg agaatgatta tcaaattcat
    36301 ttaaatggat tgtggtgatt tctctgatag accaagatta tgattacgaa aagattgatg
    36361 acttgaatgt tatggttatg tttaatgtta gcacatttac ataactttca gcttccatac
    36421 acaacatagc agaaatgtat gacagatcac tatttttgaa gcctgtcaca ggacgtcatt
    36481 atagtgtgtg tcagatctcg ttttccttaa ccatgttaca tagaatgtgc acggaaattt
    36541 aacctgcctc atatttggag caaatatgga ccgcgtcctt cattttgtac tggcacttgc
    36601 cgttgttgtg attctcgcac tgctggtaag cagcgaccgc aaaaaaattc gtatccgtta
    36661 tgttattcaa ctgcttgtta tcgaagtgtt actggcgtgg ttcttcctga actccgacgt
    36721 tggtttaggc ttcgtgaaag gcttctccga aatgttcgaa aaactgctcg gatttgccaa
    36781 cgaagggact aacttcgtct ttggtagcat gaatgatcaa ggcctggcat tcttcttcct
    36841 gaaagtgctg tgcccaatcg tctttatctc tgcactgatc ggtattctcc agcacattcg
    36901 cgtgttgccg gtgattatcc gcgcaattgg tttcctgctc tccaaagtca acggtatggg
    36961 caaactggaa tcctttaacg ccgtcagctc cctgattctg ggtcagtcag aaaactttat
    37021 tgcctataaa gatatcctcg gcaaaatctc ccgtaatcgt atgtacacca tggctgccac
    37081 ggcaatgtcc accgtgtcga tgtccatcgt tggtgcatac atgaccatgc tggaaccgaa
    37141 atacgtcgtt gctgcgctgg tactgaacat gttcagcacc tttatcgtgc tgtcgctgat
    37201 caatccttac cgtgttgatg ccagtgaaga aaacatccag atgtccaacc tgcacgaagg
    37261 tcagagcttc ttcgaaatgc tgggtgaata cattctggca ggtttcaaag ttgccattat
    37321 cgttgccgcg atgctgatcg gctttatcgc cctgatcgcc gcgctgaacg cactgtttgc
    37381 caccgttact ggctggtttg gctacagcat ctccttccag ggcatcctgg gctacatctt
    37441 ctatccgatt gcatgggtga tgggtgttcc ttccagtgaa gcactgcaag tgggcagtat
    37501 catggcgacc aaactggttt ccaacgagtt cgttgcgatg atggatctgc agaaaattgc
    37561 ttccacgctc tctccgcgtg ctgaaggcat catctctgtg ttcctagttt ccttcgctaa
    37621 cttctcttca atcgggatta tcgcaggtgc agttaaaggc ctgaatgaag agcaaggtaa
    37681 cgtggtttct cgcttcggtc tgaaactggt ttacggctct accctggtga gtgtgctgtc
    37741 tgcgtcaatc gcagcactgg tgctgtaaaa ccatacataa aaaagccggg gatcattccc
    37801 cggctttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa atacccttgc
    37861 agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc gacaaactcc
    37921 gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac aatcatcgca
    37981 tccagcgtat tcgtgacaat atctttcaca aagacgccat caattttgat gatatcagcc
    38041 tgcaaacgct ttaaccgttc gtagttggca tatccggtgc caaagtcatc aatcgcaatc
    38101 cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac
    38161 gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata acgcttaaac
    38221 agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat taaattgact
    38281 gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc
    38341 agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg cagaaactta
    38401 tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc ataaccttca
    38461 ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc taacgccgtg
    38521 cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat ctcctctctg
    38581 ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca gcttaactgc
    38641 cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc agcaccatag
    38701 cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat gttaaccata
    38761 tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc actacccggc
    38821 aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca aattgagcgg
    38881 atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag attatcaatg
    38941 cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa cgcacgaaag
    39001 ttgggtagga gcgttaacgg atctgttagc gcctgcaaat gccagcggcg attaagccat
    39061 tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga aatcagcacc
    39121 gcgagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt ctggttgtaa
    39181 ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata gcgaagatta
    39241 ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc aataaagtcg
    39301 ttttcataag gtgtgcacaa cagaagtagc agcacgctta acgctgccag ccagcttaag
    39361 gtaaatgcga gtgagatagt agaaaagcat gttgtaaatc agcactgcgg tgaaaaggct
    39421 tagtaaatca acgaccgtga aaatggcatc cgcatcgccg aaaaaggtgg atatctttag
    39481 cgggaaatca aagaagctcc ccacaagata catgctgcat ttgatgccga tcggcgtcac
    39541 caaaccaagc cagaacagac gttgccagac atatcggctg gtcaatccat aacgccaacg
    39601 cgtccccagc tgccagcgta atattgcgca ggcacacaac acgacaaacg tctggctgcc
    39661 gagcatgacc gcagtctgta aaaaagagag atgaaagttc cacagattgg tgcaaaacat
    39721 cccgcataaa acgggaacga caccgcgcca gccaaagata aacaagattg acagcattac
    39781 gcacagtggc atccacgcca gaaagatata actggaatgg ataatggcaa gtggcgaaat
    39841 aaaacgggat agctgaataa gtaccacggt gagcgtaaac gctagtgtga atatcttgat
    39901 attttttatc aggttatgct ccacaaacat gagtacacta ctctcactga tcggggtttt
    39961 caggcacaca aataataagt ttattcgcat cgcgaagcaa acaggggcca gcctgttgcc
    40021 gatttttcat ccaatgatgc tttcaccagg cggcgctaac ccggacgtag actgtattac
    40081 aaaagcggca aaaagcagag acaaaaaacc cccgctttgc agcgagggtt ggaaatttgg
    40141 tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct
    40201 gagctatagc cccacgatgc gtttacgtac caaatttgct gggtgcaaaa tttggtggag
    40261 ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct
    40321 atagccccgt cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga
    40381 atgtgtcaac ggcaaattgc aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac
    40441 aattttggaa aaccgctcgc attcagtagt tattcatgtc acggtttcct gtaaagtggt
    40501 gttataaaat gaactactaa tagacccaca tacattcagg gaattgttat gttcaaggag
    40561 aggatgacac cagatgaact tgccagactg accggctata gccgccagac cattaataaa
    40621 tgggtacgca aggaaggctg gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc
    40681 cgactggttc acgtcaatga acaggttcgt gaatatattc gcaatgctga acgtccagaa
    40741 ggtcagggag aagcgcctgc cctttctggt gatgcgccgc ttgaagtatt gctggtgaca
    40801 ctggcgaaag agatgacgcc agttgaacaa aaacagttta catccttgct tctgcgggaa
    40861 gggattatcg gattgttaca acgcttaggg attcgcgata gcaaataata tgaaaagatt
    40921 acgcaataaa atgaccaccg aagaactggc tgaatgcctc ggtgtggcta aacaaactgt
    40981 taaccgttgg accagagaaa aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg
    41041 tcgtgccaga cttattctgg tcgatacgca agtttgcgag tttattcaga acacgccagc
    41101 cttccataac acaccaatgt tgatggaagc tgaagagaga attgctgaat acgctcctgg
    41161 agcacgcgcc ccggcgtatc gacaaatcat caacgcgata gataatatga cagacattga
    41221 gcaggaaaaa gtcgcgcaat ttttgtcacg tgaaggaatt cgcaatttcc tcgcccgtct
    41281 cgatattgac gaatcagcat aaataaaaaa cggcaggata ttatctcctg ccgtttatct
    41341 ttttacacgc taattactgc tgattttcgc gttcagcaat aaaatccagc gctttgttga
    41401 tacgctcgat actgcgggtc ttaccaattg cgtgaacggt aacatccagt gctggagact
    41461 gccccgcacc ggttacggcg acacgcagcg gcataccaac tttacccata cccacttcca
    41521 gctcatccgc cgtcgcctga atagcgtgat gaacgttttc agcggtccag tcagtaatcg
    41581 cggccagttt gtcacgaacc acttccagcg gctgacgcgc taccggacgc agatgttttt
    41641 tcgccgcatc ggcatcgaac tcagcaaaat cttcgtagaa ataacggcag ctctgtgcca
    41701 tctctttcag cgtcttgcag cgttcgccca gcagtttcac cagatcagcc agctgcgggc
    41761 cgttacgggt atcgatattt tcctgctcaa tgtgccactg taagtgagta gcaacatact
    41821 ccggcggcag cgcgttaatg tagtgatggt tcagccacag cagcttgtcg gtgttgaacg
    41881 cactggcaga tttgctgacg gcattcaaag tgaagtattt gatcatctct tcacgagtga
    41941 agatttcctg atcgccgtgg gaccagccca gacgcaccag atagttcagc agtgcttctg
    42001 gcaaataacc gtcatcacga tactgcatta cgctgactgc cccgtgacgt ttggacagtt
    42061 ttttaccgtc atcgccattg atcatagaaa cgtgcgcgta aaccggcacc ggcgctttca
    42121 gggccttaag aatgttgatc tggcgcggcg tgttgttgat atggtcttcg ccacggataa
    42181 cgtgggtgat ttccatatcc cagtcatcga caaccacaca gaagttatag gttggggaac
    42241 catcggtacg gcggataata agatcgtcca gttcctggtt gctgaactcg atcggaccac
    42301 ggatctgatc gtcaaaaaca acagaacctt cctgcgggtt agcaaaacgt acaacacacg
    42361 gttcatcatc agcatgatgc tcatggctgt ggcggcagcg accgtcataa cgcggcttct
    42421 cacctttcgc catttgctct tcgcgcagcg cttccaggcg ctctttagag caatagcatt
    42481 tataagcagt gccctcttcc agcatctgat cgatcaccgc gttgtagcga tcaaaacgtt
    42541 tggtctggta gtacggacct tcatcccact ccaggctcag ccagttcatg ccatccataa
    42601 tggcttcgat agcttccggc gtggaacgct caagatcggt gtcttcaata cgcagcacga
    42661 actcaccgcc gtggttacgt gcaaaaagcc aggagtaaag agcagtacgc gcgccgccaa
    42721 cgtgcagata gcctgttggg cttggcgcga agcgagtttt gattttcatg aaatggcctt
    42781 acgtttagaa agatgccgac aaccggcaaa tcctggaaaa attgagtggg cgatattcta
    42841 tcactcccgc ctgattcctc aatgtagttc gggtttctac cttacgcatt attgttttcg
    42901 ttgacaaatt gcgcaacacg gtctgttttg cgatcatctt gcttaaattt acgacgaacg
    42961 aacaatttct ttagaaaatg cgttgactca ttttgaactc tccctataat gcgactccac
    43021 acagcggggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    43081 ttcgatcccg tcatcaccca ccaactactt tatgtagtct ccgccgtgta gcaagaaatt
    43141 gagaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    43201 ttcgataccg tcatcaccca ccacttcggg tcgttagctc agttggtaga gcagttgact
    43261 tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct
    43321 aaaggcgctt ttttgctatc tgcgatactc aaagattcga acctgcagca ggtttgagtt
    43381 gagcgcagcg aaacaacgga gccgctcgcg gcgacggccc gaagggcgag cgaagcgagt
    43441 catcctgcac gacccaccaa tgcaaaaaag cgccctaaag gcgctttttt gctatctgcg
    43501 atactcaaag attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg
    43561 ctcgcggcga cggcccgaaa ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgca
    43621 aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgattt gtaagattga tttttcttct
    43681 caagttcctt gttcttctac cgatattacc tcgtacagtg acgaggactg aacaatggcc
    43741 actatctctt caacttcaat tccttccata caaacacaga gtagtaacag ggcatcacaa
    43801 ggtagcgatg ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa
    43861 caaattaaag agattgtaga cactagcggc agtgctgaag acaagcaaaa acaggccgaa
    43921 ttgattcaac agcaaatcac cctactggaa acacaactgg cgcagttgca gaaacagcag
    43981 gcagaaaagg cgcaggagaa agagcagcgt ttgtctctga atgtatccct tctaaatcct
    44041 gttgaaaaaa cgactcatat cgatatctat atctgataca acggatcccc ttcccgacca
    44101 gccattagcg cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa
    44161 agtgaacggt tcttcctgcg caccagcatc aactggcgtt caaccaccgg cgtaatgcgc
    44221 ttaaccacca gtgggctacc ttcaggcaat ggtagcgcca gtgcaggaag aatactgatg
    44281 ccgatgcctg cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata
    44341 ttcgcctgaa taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg
    44401 taatcctgca acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg
    44461 gccaaagcgc tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat
    44521 tgcagatcgc ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga
    44581 atactttcca tcacccactg ctgcggacga tcatgcagga caaactgaat atctggatag
    44641 cggcgatggc tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca
    44701 gcgacccgaa cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg
    44761 ttaagttcat ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc
    44821 accacttcac gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc
    44881 acactgtgac ttactgccga ctggctcaag ccgatacgct ctcctgcacg actaaaactt
    44941 ttctcctgcg ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt
    45001 cttcatgaat ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa
    45061 ccatcgatca tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac
    45121 cctgatcacg gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt
    45181 ctttgaaaat ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt
    45241 gtcgcgtgag gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg
    45301 cagcaccttc gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa
    45361 tgtcgacccg atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca
    45421 gtctgcaatc gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc
    45481 gtcagcatcc agcctgctgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa
    45541 tgttcacggt gcaaggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct
    45601 gccgtttgtg ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa
    45661 aaaatggatt gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag
    45721 cgaagccgtc gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt
    45781 ggtggtcagc tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg
    45841 actgagcttc aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct
    45901 ggcaaatggc atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt
    45961 gctgcccctg atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg
    46021 atacaaacgc cagaccgaac agttacaggc gcagcaggaa agcagcgccg ataaagctta
    46081 aagcggacgc ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt
    46141 gatttgcatt agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag
    46201 cagatactcc tccggcaggt caattaagcg acgccctttg tatttgccaa acggcattat
    46261 cgtattggct atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca
    46321 tttccgcttc gtcgatgact tcaatgccca gttcctgcgc cttcgccagt ttagatcctg
    46381 cagcttcacc cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc
    46441 ccagttcgac cagtcgagct ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa
    46501 gcaccacggt tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggcg
    46561 caggccagtg aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg
    46621 caaagaagtt gtgaacgtgg gatgcaacga caatgccaac atcaggcacc ttttgcagct
    46681 cttcaatcga agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg
    46741 cggtggcctc gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt
    46801 ctttcgcttt ttccagcgcg ttaaccacgt tttgtgccaa ttttggcccc atacgctcca
    46861 gtccggtcag tttgcctgcg gtgagtttga acagatctgc cggagtatgg acatattctt
    46921 tttcaaccag ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg
    46981 aaacaaagtg tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc
    47041 gggcaaccgc ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg
    47101 ggaatacaac ctcacgggta tcttccgggc gttcagaaag cacgacgtta accacctgcg
    47161 ggatcacgtc gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt
    47221 cgatttcatc cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag
    47281 gttccagacg cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac
    47341 gcacaaaggt catctgctcc tgcgccggga atttaaacgc aaccgcccag cgcggggcac
    47401 gcgcgacaaa gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt
    47461 cgatatcaaa gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt
    47521 cttccgccga ttcacaaagc gttacccgat cgctgaccgg caccccccac tttttaaatt
    47581 gcagtaaacg gccaagatga gtatccggca gctcgccacc ttccagaaca ccaacgccat
    47641 agcagaaaaa agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac
    47701 cagctgccgc attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt
    47761 taatcttttc gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg
    47821 ggatattctc tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga
    47881 tatcttcccc ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat
    47941 acagaatact gacggcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt
    48001 tgtttttcag acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa
    48061 cgttatccag tgacagcatt ggtacttcat ggcgtatctg gctgaaagct gccagcggcg
    48121 cagcacctac acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca
    48181 gctcgcgcag ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca
    48241 tcacatgata aagatattca tgatggcaaa gcgtcgttcg cagttctgtc agttgttgtt
    48301 cgattgattc catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga
    48361 ggagttaatt tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc
    48421 tggtactcgc gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg
    48481 cccacttcat cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg
    48541 tcaccgtaag acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt
    48601 tcaggatcaa aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg
    48661 ccatccgggc taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt
    48721 tgaatgctgt taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc
    48781 atgataatca ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca
    48841 ggctcaggct gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc
    48901 ggtgctgaat gcacaggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac
    48961 ggctgttcag gctgcggctg ataggcaggc tgctgcaccg gctgcgctgg acgcggagca
    49021 tgttgcggcg gtacctgcgc ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac
    49081 gcataaggcg gtaggtactg gtgttgcggc gacggacgag cagcctcatg ctcctgagcg
    49141 ttagccgggg catgattcac gcggtgaaca cgaacctcac caacgccctc atcatcttcg
    49201 acatcctcgt cataagaatc gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg
    49261 cggaacatag aagatcgttc tttacggctg gtccagaaac catgtaccag taaagcgatt
    49321 atggcgatcg cgccaacaat gattaatatc agacgcaaat cctgcatcat tatattctct
    49381 gttgttctaa caccttgcca ccacggcaaa catttactca ctaagagtat ttgccgatta
    49441 cctcaagtgc aagtgcacta ttaactttca cagcacaaag atagatgaaa tcgtgctttt
    49501 tgctgttttt tcgaacatat cctaactgtc cattgcgcaa ttacccggta aaatacgcag
    49561 aattttcctg ggattggtca aaaggagctc atcctgatta tggtttcatc attcacatct
    49621 gccccacgca gcggttttta ctattttgcg caaggctgga agctcgtctc gcaacctggg
    49681 attcggcgtt tcgttatttt accgctgctg gtcaatattt tgttgatggg gggcgcattc
    49741 tggtggctct ttacacagct cgatgtctgg atcccgactt tcatgagtta cgttccggac
    49801 tggctacaat ggctgagtta tctgttgtgg cctctggctg tcatctctgt gctgttagtg
    49861 tttggctatt tcttctccac gattgctaac tggattgccg ctccgtttaa cggtttattg
    49921 gctgaacaac tggaagcacg attgactggc gctacaccgc cagataccgg gattttcggt
    49981 atcatgaaag atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgt ctggtatctg
    50041 ccgcgcgcaa ttgtattact aattctttac ttcatccctg gtattgggca aaccgtcgcg
    50101 ccggtactgt ggttcctgtt tagcgcctgg atgttagcca tccagtattg cgattacccc
    50161 ttcgataacc acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc
    50221 accaatatgc agtttggtgc tttaaccagc ctgtttacga tgatcccgct gcttaatctg
    50281 ttcatcatgc ccgttgccgt ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat
    50341 aaacacgcga tgtggcggta acaatctacc ggttattttg taaaccgttt gtgtgaaaca
    50401 ggggtggctt atgccgcccc ttattccatc ttgcatgtca ttatttccct tctgtatata
    50461 gatatgctaa atccttactt ccgcatattc tctgagcggg tatgctacct gttgtatccc
    50521 aatttcatac agttaaggac aggccatgag taagattttt gaagataact cgctgactat
    50581 cggtcacacg ccgctggttc gcctgaatcg catcggtaac ggacgcattc tggcgaaggt
    50641 ggaatctcgt aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga
    50701 tgccgaaaag cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa
    50761 taccgggatt gcactggcct atgtagctgc cgctcgcggt tacaaactca ccctgaccat
    50821 gccagaaacc atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt
    50881 gctgacggaa ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc
    50941 cagcaatcca gagaaatacc tgctgctgca acaattcagc aatccggcaa accctgaaat
    51001 tcacgaaaaa accaccggtc cggaaatctg ggaagatacc gacggtcagg ttgatgtatt
    51061 tattgctggc gttgggactg gcggtacgct gactggcgtc agccgctaca ttaaaggcac
    51121 caaaggcaag accgatctta tctctgtcgc cgttgagcca accgattctc cagttatcgc
    51181 ccaggtgctg gcaggtgaag agattaaacc tggcccgcat aaaattcagg gtattggcgc
    51241 tggttttatc ccggctaacc tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa
    51301 tgaagaagcc atttctaccg cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat
    51361 ctcttctgga gcagctgttg ccgcagcgtt gaaactacaa gaagatgaaa gctttaccaa
    51421 caagaatatt gtggttattc taccatcatc gggtgagcgt tatttaagca ccgcattgtt
    51481 tgccgatctc ttcactgaga aagaattgca acagtaatgc cagcttgtta aaaatgcgta
    51541 aaaaagcacc tttttaggtg cttttttgtg gcctgcttca aactttcgcc cctcctggca
    51601 ttgattcagc ctgtcggaac tggtatttaa ccagactaat tattttgatg cgcgaaatta
    51661 atcgttacag gaaaagccaa agctgaatcg attttatgat ttggttcaat tcttccttta
    51721 gcggcataat gcttaatgac gtacgaaacg tcagcggtca acacccgcca gcaatggact
    51781 gtattgcgct cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata caggctaaag
    51841 tcgaaccgcc aggctagact ttagttccac aacactaaac ctataagttg gggaaataca
    51901 atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc
    51961 cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa
    52021 agcgccagcg cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt
    52081 gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg
    52141 atggcggaac tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag taaggtaggg
    52201 ttatgatttc aggcatttta gcatccccgg gtatcgcttt cggtaaagct ctgcttctga
    52261 aagaagacga aattgtcatt gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag
    52321 ttgaacgttt tctgagcggt cgtgccaagg catcagccca gctggaaacg atcaaaacga
    52381 aagctggtga aacgttcggt gaagaaaaag aagccatctt tgaagggcat attatgctgc
    52441 tcgaagatga ggagctggag caggaaatca tagccctgat taaagataaa cacatgacag
    52501 ctgacgcagc tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa gagctggatg
    52561 atgaatacct gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca
    52621 acatcctggg cctgaagatt atcgacctga gcgccattca ggatgaagtc attctggttg
    52681 ccgctgacct gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg ctgggtttca
    52741 tcaccgacgc gggtggccgt acttcccaca cctctatcat ggcgcgttct ctggaactgc
    52801 ctgctatcgt gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac tatctgattc
    52861 tggatgccgt aaataatcag gtttacgtca atccaaccaa cgaagttatt gataaaatgc
    52921 gcgctgttca ggagcaagtg gcttctgaaa aagcagagct tgctaaactg aaagatctgc
    52981 cagctattac gctggacggt caccaggtag aagtatgcgc taacattggt acggttcgtg
    53041 acgttgaagg tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt actgagttcc
    53101 tgttcatgga ccgcgacgcg ctgcccactg aagaagaaca gtttgctgct tacaaagcag
    53161 tggctgaagc gtgtggctcg caggcggtta tcgttcgtac catggacatc ggcggcgaca
    53221 aagagctgcc atacatgaac ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta
    53281 tccgtatcgc gatggatcgt aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg
    53341 cctcggcttt cggtaaattg cgcattatgt tcccgatgat catctctgtt gaagaagtgc
    53401 gtgcactgcg caaagagatc gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt
    53461 ttgacgagtc aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca acaattgcac
    53521 gtcatttagc caaagaagtt gatttcttta gtatcggcac caatgattta acgcagtaca
    53581 ctctggcagt tgaccgtggt aatgatatga tttcacacct ttaccagcca atgtcaccgt
    53641 ccgtgctgaa cttgatcaag caagttattg atgcttctca tgctgaaggc aaatggactg
    53701 gcatgtgtgg tgagcttgct ggcgatgaac gtgctacact tctgttgctg gggatgggtc
    53761 tggacgaatt ctctatgagc gccatttcta tcccgcgcat taagaagatt atccgtaaca
    53821 cgaacttcga agatgcgaag gtgttagcag agcaggctct tgctcaaccg acaacggacg
    53881 agttaatgac gctggttaac aagttcattg aagaaaaaac aatctgctaa tccacgagat
    53941 gcggcccaat ttactgctta ggagaagatc atgggtttgt tcgataaact gaaatctctg
    54001 gtttccgacg acaaaaagga taccggaact attgagatca ttgctccgct ctctggcgag
    54061 atcgtcaata tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt
    54121 attgctatca aaccaacggg taacaaaatg gtcgcgcctg tagacggtac cattggtaaa
    54181 atctttgaaa ccaatcacgc gttctctatc gaatctgata gcggcgttga actgttcgtc
    54241 cacttcggta tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa
    54301 ggtcagcgcg tgaaagttgg cgatactgtc attgaatttg atctgccgct gctggaagag
    54361 aaagccaagt ctaccctgac tccggttgtt atctccaaca tggacgaaat caaagaactg
    54421 atcaaactgt ccggtagcgt aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa
    54481 ttcttgccgc agtgaaaaat ggcgcccatc ggcgccattt tttatgcttc cgccagcggc
    54541 ggcaaaatca attcatcgct ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc
    54601 aaccccgcac ggtgcaccgc atcgtttaac gccttccctt tcagcaaacc actgataagc
    54661 tgagcacaaa acaggtcgcc agtccctttc aggtcggttt ttacccgtga atgggaaatg
    54721 acattcacgc tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattaccg
    54781 gaggcgctgg taatcaccac ccattttaat gtgtctgaaa gcagactttt tgcggcagca
    54841 atggcactgt cgagatcgcg gcaattttta ccggtcagga tttccagctc aaagatattg
    54901 ggggtaattc cctgcgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt
    54961 ttgacataaa ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca
    55021 ggatggtctt tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt
    55081 cccatatagc ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagcgca
    55141 cgcaaatagc cgctaaacca ttcgtccgga atcgcaccac cgtagaaagt gtcataatgc
    55201 ggcgtattgc tcagcaatac cgtcggcacg gcaaagacat tcaagccgtt ctgttttata
    55261 gcaggcaccg caatgctgtt gcctacgctt ccgtacacta cctgcgactg cacggcgacg
    55321 atatccgcct gcagcgccct actcttatcg ttaaacaaca acaaactact catttaattt
    55381 tttctccttg ccgatgatcc tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt
    55441 caaatcggct attacaaaac aaagataagg taattcagtg aagaaaatca tttgtctggc
    55501 cattacgctg ctaatgacac tccccgctta cgcgaagtta actgttcatg aagaagcccg
    55561 catcaacgcc atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg
    55621 tgatgaacat acctgcgatg aagcggtttc ccacctgcgt ctgaagctcg gcaatacccg
    55681 taaccgcatt gacactgccg agcagtttat tgataaggta gcttcgtcgt catcgattac
    55741 cgggaagccg tatatcgtga agatccccgg taagagcgat gagaacgcac agcctttttt
    55801 acatgcgtta attgcgcaga cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt
    55861 ggtaaggcat ttacaggagg taacatgaaa aaatgcttta tttatcacga tgaaaaatcg
    55921 aataaatttt ggtggataga ttacgaaggg gatagtttag ctgtcaacta tggcaaggta
    55981 ggtagtattg gtaaattcca tacaaaagaa ttcgataatg aagaacagtg tctgaaagaa
    56041 gccagtaaat tgattgccgc aaaaatgaaa aaaggatatc aagaaactcc agactttaac
    56101 ttcatggatc tctactattt tgatgatgaa gagattgggt tacatcttaa aacgtcacac
    56161 ccaaacttcc agtgccattt tactgatcca ctttatatgt gttgctggga tgaagaatct
    56221 ccttttggta gcgatgaagg tgctgatgct ctaaacgaac ttgaaaatag cctccgttct
    56281 ggactgtgct gatttccctc aaatgttaat tgaaactatg tggggtatga aatacatcgc
    56341 tatggacagt attcttgaag aggatgtgcg tgcgcaatta ctagccgatg aaatgagctc
    56401 tatccagagc aatatgatta cctacgcgac tgcattcggt cagatcaaag tcatgggtaa
    56461 aatctcccat aaacttaaaa agatgggact caatgcacta gcgcgtcatc agcttaccgc
    56521 aaaaattctt caatggggtg acggtcagga ctcaccaata cttcaaaaaa tgattgatga
    56581 ccttactgct tttccacacg aaaattaaaa actgcatttg tcggcagcaa caactgccga
    56641 caaatcagat tattgacaaa gtgcactttg tttatgccgg atgcggcgta aacgcctgat
    56701 ccggcataaa aatattgcta attcaatata ttgcagaaac attgtaggcc tgataagcgg
    56761 agcgcatcag gcagttttgc gtttgtcatc agtctccaat gctattaatc cttaaatccc
    56821 tgccccctga ctaaaatgct cttccccaaa cactccggtg gagaggtagc gatcgccacg
    56881 atcgcagatg atcgctacca ccaccgcacc agggttagct tttgccaccc gcagtgctcc
    56941 agcaaccgcg ccgcctgagc tgacgccaca gaatattcct tcccgcaccg ccagttcgcg
    57001 catggtgttt tccgcatcgc gctgatgaat atccagcacc tcatccacca gcgcagcgtt
    57061 gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg cccggaatac tgctgccttc
    57121 ttccggttgc aggccaacaa tggtcaccgg tttggattgt tcgcgcataa agcatgaaac
    57181 gccggtgata gtgccggtcg tccccatgct ggagacaaaa tgagtgatgc gcccgccggt
    57241 ttgctgccag atttccggcc cggtggtggt gtaatgcgca taagggttat cgggattatt
    57301 gaactgatcg agcagctttc cttcgccacg attcgccatc tccagcgcca gatcgcgcgc
    57361 accttccatg ccctgctctt tggtgacaag aatcagttcc gcaccataag cacgcatcgc
    57421 cgcacggcgt tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttcag
    57481 cgcggcaatc attgccagcg caatgccggt gttaccactg gtggcttcga ttaagacatc
    57541 acccggttta atttccccgc gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc
    57601 tttcaccgaa cctgccgggt tattgccttc cagttttaac cacacttcac tgccgttatc
    57661 cggccccatt cgctgcaact tcaccagagg cgtattgcct attgtttgtt ctaatgtact
    57721 cacaatctct atccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct
    57781 acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta
    57841 acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa
    57901 tacgctcgtc gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccat
    57961 gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg
    58021 gctgcaccac taattgggtg tagtgacctt tcgggctggc ttccagtacc tgtaccggca
    58081 gcggcgaatc gagactggta cggcggctga tatccacttc ccacgggcgc aggaagagat
    58141 ccaccggccc ctgatacgca ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga
    58201 actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa
    58261 aacgggtcgc tggttcgcgc catacctgat tcggcgcatc agcctgttca atattcccct
    58321 gactcatcac cactacacga tcggctactt cggtcgcctc ttcctggtcg tgggtcacaa
    58381 acacgctggt gaatttcagc tcttcatgca gttgacgcag ccagcgacgc agctctttac
    58441 gcacctgcgc atccagcgcg ccgaacggtt catcaagcag cagaatttgc ggttctacag
    58501 ccagcgcgcg cgccagcgcc acacgctgtt tctggccgcc ggaaagctgc gccggataac
    58561 gatccgccag atgcgcaagc tggaccattt ccagcaattt tgtcactttc gctttgatgg
    58621 ctgcggcatt cgggcgctcg cgacgcggca gcaccgtcag gccaaaagtg atattgtcga
    58681 ataccgtcat atggcggaac agcgcgtaat gctggaacac gaaaccgact ttacgatcgc
    58741 gcgcgtgcag gcgactcacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct
    58801 ccagcccggc gataatccgc agcagcgtgg ttttcccgga accggacggc cccagcaacg
    58861 cgaccatctg accagaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa
    58921 aagacttctt gatattggca atctcaatgc tcatgatgct cctcctgctg cgcgcgtttt
    58981 tcctgattct ccaggcgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc
    59041 aacgtcaaca gcgcggcggc ggtaaaggag ccgacggtgt tgtagtcctg ctccaacaat
    59101 tcaatctgta acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacact
    59161 gcaccaaact cgccaatcgc gcgggcgttg gtcagcacca cgccgtaaag cagcgcccag
    59221 cgaatattcg gcaatgtgac gcgacggaac atctgccagc cggacgcgcc aagcaaaatc
    59281 gccgcttcgt cttcctggct gccctggctt aacatcaccg gcaccagctc gcgcaccaca
    59341 aacggacacg tcacaaagat ggtgaccagc accattccgg gccaggagaa cataatttgc
    59401 agattatgtt catccagcca gccgccgagc gggccgttgg agccgtagaa cagcagatac
    59461 accagaccgg caaccaccgg cgatacagca aacggaatgt ccagtagcgt cagcagtaac
    59521 tggcgtcccg ggaagttaaa gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg
    59581 tttaccggta cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc
    59641 ggatcggcca gattctgtaa aaccggcatc agccccttgc tgaatgcctg cacgaagatg
    59701 taaatcatcg gcaccagcag gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac
    59761 catttgcccc agttaatcgg gcgcgcgtca taacgcttca attgggtaac ttccgccatt
    59821 aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca
    59881 gcagagatgc cgcgaggatc accgaagcaa tcgcgctcgc tgccgggtaa tcaaactcct
    59941 gtaagcgcac aaaaatcatc agcgacgtca cttccgtctt ccacgcgata tttccggcga
    60001 taaaaatcac cgcgccaaat tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca
    60061 ccagcgccgg agaaagctcc ggcagcacca ctttgcagaa actctgccag cgcgttgcac
    60121 caagcgtttc cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg
    60181 tacgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatacca agccaggtat
    60241 aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttt acggaaaaga
    60301 gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc aaagggtgaa tccatcagcg
    60361 catcaagcag cgtgcggccc ggaaagcgat aacgtgttaa aatccacgcc atcagcagac
    60421 cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag cagcgtcact ttgtaggccg
    60481 cgaccacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc tcagaaagct
    60541 gcatcaccag cgcagagagc ggcagcagca atatcaggca cacaaacagc agactggtgc
    60601 caaggcttaa agtaaagccc ggcagcacgc gtcttgaaga aactgcaaac attacttacg
    60661 ccccgccgct aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc
    60721 cggccatgag ccaaatttgt cttccacgcg gaacagttcg gtctgcggga atttatcttt
    60781 cagtttgtcc atgacttccg ggttattcac acggtagtaa tagtcggtga tgatggtctg
    60841 cgcctgtggg ctgtagagcc agttcaggta ggcttttgcg gctttttccg taccgttggc
    60901 ctgcacattt ttatcgaccc acgccaccgg gaattccgcc agaatattgg ttttcgggat
    60961 caccacttca aagccctgtg cttcatactg tttacggatg ttgttcactt ccgattcaaa
    61021 gctaatcagc acatcgccca agccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc
    61081 ggtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt
    61141 atcgccaccg tcagctttat ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt
    61201 acccgacgtt ttcgggttcg ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca
    61261 gtcgtggata ttcttcgggt tgcccttacg caccaggaag cccatggtgg agtagaacgg
    61321 cgagctatta ttcggcaggc gcgactgcca gtcagccggg atcagcttgc ctttgtcgtg
    61381 caggatttgt acgtcggtca cctggttata agtgacaacg tctgctttta agccctgcaa
    61441 aatcgccagc gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc
    61501 gccgttatct tttgcccatt gctgttcaaa cggcggattc agggcggcaa acagctcgcg
    61561 ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag
    61621 agaagcgacc agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta
    61681 taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt
    61741 tttatatacc gtttggtgat ttggaagtta aaaagggaat aagaacttgg ttcaacaggc
    61801 gtaattgttg caggcagttt ggacacggac agcgcggaga aaccggagcg tacacatagt
    61861 acgtgaggat ttcgagcact gcccggggcc aaaatgacaa atgaaatagc ctggtgaact
    61921 tagttcaaga cgaaatcctc cccacacatg cagggaggaa acagaggtga atcagatacc
    61981 gacgctaacc gtctccggca gtgtgctgcc gccatcaatc acattctgtg taccggttaa
    62041 atagctggat tcatccgatg cgaggaaggc cgccagttcg ccgacttcca gcggatcggc
    62101 gaggcgacac agcgggattg cttttgccat ttcagtcagc accgattctg gatcttccgg
    62161 gttcgactgg cgggcaatgc tttccgccat tggcgtgcgg acgtatcccg ggcagatggc
    62221 gttaacgcga ataccggatt gcgcgtactc caccgccagc gatttagtca ggccaacaat
    62281 cgccgctttc gttaaggcat acgccgtttc gccaggatcg gccaccatat caccagtgac
    62341 tgaagacatc atcacaatgc gaccatcttt gcgcgcaatc atctccggca gcaccgcctt
    62401 cgtgacgttc catacgcctt taatattgat atcaatatgg aaatcgcgat cttcatcgct
    62461 catatcgagg aaactgccca gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg
    62521 cccttctttt tccttcgcgc gtttgatagc tgcggctacc gacgccgggt cacgcacatc
    62581 ggcgacaacc gccgtacagc gatgaccacg accacacagt tcgtccgcta gcttttcgat
    62641 ctcaggggag atatccagca agattaggtt cgcgccatga cgtgcaaaag ttctggcaat
    62701 tccttcgcca attccctgca atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc
    62761 cattttaatg ctctccttgt taatacagag cgttacactc cattccctct gaaaatacag
    62821 gataaacaaa atattgctgc ggatcagctc actaattcag gaaaatctca atccagtcgc
    62881 tgctttatgc atcatttcag gcgttgtgtt agctcactgc tgcgctgaat cattttcaat
    62941 gacgccacat cattgatgtt gcacaagacc aacaaacagt aaatcggtaa cagaattttg
    63001 cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa cggtatcgag
    63061 cgtgaaatgc gccagccgcc gcagcggaga gtcagccaga gaagtaatgg caatcaccgt
    63121 tgccccctgc tttcgtgccg cttctgcgca caagacaatc tcttttttac tgccgctgta
    63181 agagatagcg atttgtacat cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg
    63241 cgtgtcggct tcgcaggcca cgcgatagcc aattttcatc agtttgaacg acagatcgcg
    63301 ccccaccagc gccgatccgc ccaggcccgt tatctggata aacggtgctt tactgatgac
    63361 ctcaatgatt ttttgcagcc gcgcgtagtc gaacaacgca caggtttgct ccagcgccag
    63421 ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac tggtaatcga
    63481 actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgccaat
    63541 taacgccata cgcaattcag taaatccttg cgcgccgagc ttctgggcaa atttcacaat
    63601 gctcgactgg ctgataccga gctgtttcgc catctgccgt gaagagacgg atttcagctc
    63661 gctcactctg gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa attcactttc
    63721 cgcattgcgt attttcgtca ggtacaacat cttccactct cctgggctgg cagcaaaaat
    63781 tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt
    63841 aagtctgtca attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt
    63901 aaggtcacca ccgatgcaac tagaaaagat gattactgaa ggctcgaaca ccgcctcggc
    63961 tgaaattgac cgcgtatcga cgctggaaat gtgccggatt atcaacgatg aagataaaac
    64021 cgtaccgcct gccgttgatc gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca
    64081 cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct
    64141 ggggattctg gatgccagcg aatgtccgcc cacctacggc gtgaaaccgg gtctggtggt
    64201 tggtttgatt gctggaggcg aatatgccat tcagcacgcg gtggaaggag ctgaagatag
    64261 ccgggaaggt ggtgttaacg atctgaaaaa tattaattta acggcacagg atgtggtggt
    64321 tggcattgct gccagcggtc gcacgccgta tgtgattgcc ggactggaat acgcacgcca
    64381 gctcggctgc cgcacagtgg gaatttcctg taatccgggg agcgccgttt caaccaccgc
    64441 tgagtttgcc attacaccga ttgtaggtgc cgaagttgtt accggttctt cgcggatgaa
    64501 agcaggtaca gcgcagaaac tggtgctcaa tatgctttcc accgggctga tgattaaatc
    64561 cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt
    64621 gcgccaggtc aatatcgtta aaaacgccac cggatgtaat gccgaacaag cggaagcggc
    64681 gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga
    64741 tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga
    64801 caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca ttcttacccg
    64861 tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg
    64921 tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt
    64981 cattttgacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc
    65041 aaaagcgatg agcgagctgc tgggcgatgc accggtacag gatgccgcag aaatcgccgc
    65101 ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgccaa
    65161 attcgccacc atcttcacgc cgctgatccc cggttttatt gccgccggtc tgctgctggg
    65221 gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc
    65281 cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcaccttcc tagtgattct
    65341 ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattattgc
    65401 cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg ccggttttca
    65461 cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc
    65521 gtgggcctgc gcccgcattg aaggcatggt gcgccgcttt atgccggacg atctcgacat
    65581 gctgctgacc tcgttaatca ccctgctgat caccgctacg cttgcgtacc tgatcattat
    65641 gccgctgggt ggctggttat tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa
    65701 tccgttcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat ttggcgtgca
    65761 tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca acagcttatt
    65821 tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg
    65881 ggcgcaaccg cacagtgcgc tacgcagtca ggtacgcggg gcgattattc ccggcctgct
    65941 gggcgttggt gaaccgctga tttacggtgt caccctgccc cgcatgaagc cgtttgttac
    66001 cgcctgttta ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct ggtggggtct
    66061 gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc cgctgatgac
    66121 ttccgcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc tggtggcatg
    66181 ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc tggactgata
    66241 ttatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc gtcagcgccg
    66301 caaaataccc tgttctgaca gaaggtagtt gatcaagtat atgcggcgtt aaagcagtaa
    66361 taaaaaggcc agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta
    66421 cagcgccatc aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt
    66481 gaaacgcaac atcgcatcac gtttgccatc ggtatcgcca aacatgctca gcagttgctg
    66541 ctcaatgtta tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc
    66601 ggtgccgtac ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc
    66661 aacgcgggtg aggtgagagg tttccggacg ttcgtcgccg tcaatctctt cgttggcctc
    66721 tttggtgcgc ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag
    66781 gttgtgctcc caacgctgca caaacacata gctgccgccg gaatccacgc cgtctttgat
    66841 aaccgccact tcgcggcgcg tctcttcacc cgccgggttt tctgtaccgt caacaaagcc
    66901 gctcaggtca cgctcttcca cccaacggaa gccgtggatc tcttctttca cttcaatgca
    66961 gtcaccaaag gcttccatcg ccgcctgagc gacagagaag tttacgtcgt gacgcagaga
    67021 aagaatgtgg atcaacacat cgaactgggt cgtcggcgca aggcctttac cgtagcccgg
    67081 aaaatctttc agctcttctg ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc
    67141 aaaggcaacc accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt
    67201 gtcggcaaat gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc
    67261 gatccaaatc gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt
    67321 tcctcctgaa aataagaatg ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt
    67381 tttttcgcga cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact
    67441 ttccagtttt tcagggtatc gtccgaaggc attaaccctt caggaccacg ccattccccg
    67501 ctgaactggt agctaatatg ttgactgcct tcggctttac attcgacggc acgattatca
    67561 tcaccatcag ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta
    67621 ccgattttaa caccggtgtc agcaggaata tcgctatcca gcacgtcaat gcgactgatc
    67681 gtgccttgat cgccgttaat caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag
    67741 cgcaccacgt tgccgttcgc ggttttcatt ccgctgcgca ggcgataatc gccatcaagc
    67801 gcatcagcaa tggcttgttc ttgcagtggt gtggacgccg ttaattcacc cacgccctgc
    67861 tcgctcactt tggtggacga tccaaaccag ttccacgggt tagcggcaga ccagttaact
    67921 gacgacatcg tggaacagcc ggtcagcatc aacggcatag cgcataacat taaacgcagc
    67981 gatttcatgt cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa
    68041 aagtgccgtt attctttttc ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc
    68101 cagattaacg ccacaatatc cgccactacc agcgccagac cgataccgct aacggattca
    68161 ccgttcagcc acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga
    68221 aaatagagca catgccaggt gcgaggaaac gaagcccgcc gaccgctcaa caaaaacgcc
    68281 agcaccgccg gaataccggg aatcagcccc agccagaaat tatcgtgatc gggataaaac
    68341 agatttagca gcgcagtacc ctgctcacgc gacgcaccgg caatgacaaa cagcacccag
    68401 gttcgcgcct gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc
    68461 gcatcataat ggacaggatg gaactcagta ctcttcatct tcaatcaaac gcttacccag
    68521 actcagcacg tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt
    68581 atcttccggc acgttgatct gaattttcgg gcagccgcga gcaatcagct ttttctccag
    68641 ccggttaagc aacgcattgg caattccacg cccacgaaac tctggatgca cgccaagata
    68701 ataagcagac ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cttcaccgtt
    68761 tacctcagcg accaaaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc
    68821 cggatcgttc cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa
    68881 atcttcctgg cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag
    68941 gctgattatg gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt
    69001 ggcataatgg ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac
    69061 cgtcgtaacg gattacgcga tacgatataa catctggaac tttattatta caactcaggc
    69121 cgtatgagca cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc
    69181 ggtttagctg ccctgacgtt gtcaggaatg tcgcaagcca ttgccaaaga cgaaccttta
    69241 aaaaccagca acggacacag caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt
    69301 gttctcgatc caggccacgg cggaattgat accggagcga tcggacgcaa cggttcgaaa
    69361 gaaaaacatg tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg
    69421 attgatgcgc gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa
    69481 atcgcccata aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac
    69541 ccgaaagctg ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg
    69601 gcgaaatacc tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact
    69661 gacaaggatc acctattgca gcaagtactg tttgatctgg tgcaaaccga tactattaaa
    69721 aacagcctga cgcttggctc gcatattctg aaaaagatta agccggtgca taaactgcac
    69781 agccgcaaca ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg
    69841 ctggtggaaa cctcgtttat caccaacccg gaagaagagc ggctgttagg cacggcggcg
    69901 tttcgtcaga aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc
    69961 gacaaccaga aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt
    70021 aaacagtttc tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc
    70081 gcagaatttg tcgaagatag ttggcagcgc gaagctggcg gcggcgggcg cagtcgggtg
    70141 ttgcgtaatg gtggtgtttt cgaacaggcg ggcgtcaact tttctcacgt ccacggtgag
    70201 gcgatgcctg cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg
    70261 atgggcgttt cactggtagt gcatccgcat aacccgtatg ttcccaccag ccacgcgaat
    70321 gtgcgttttt ttattgccga aaaaccgggt gcagagcccg tttggtggtt tggcggcggc
    70381 ttcgatttaa cccctttcta tggttttgaa gaagacgcca ttcactggca ccgcaccgcc
    70441 cgtgacctgt gccagccatt tggtgaagac gtttatcccc gttacaaaaa gtggtgcgac
    70501 gattacttct acctcaaaca tcgcaacgaa cagcgcggca ttggcgggct gttctttgat
    70561 gatctgaaca cgccagattt cgaccactgt tttgccttta tgcaggcggt aggcaaaggc
    70621 tacaccgacg cttatttacc aattgtagag cgacgtaaag cgatggccta cggcgagcgc
    70681 gagcgcaatt ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat
    70741 cgcggcacgc tgtttggcct gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg
    70801 ccgccactgg tacgctggga gtatgattat cagccaaaag atggcagccc agaagcggcg
    70861 ttaagtgagt ttattaaggt cagggattgg gtgtaactcc ctcaccccca ttcccgcatc
    70921 cgctgatgca acgtcaacga ctgcttctcg gcaaacagct gctggtaatc cgtggcaaat
    70981 tgccccagat gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac
    71041 cacgggctta tcagttcgcg gcgtacggcg ttcaggcgaa tgcgtttcag ccacgcgttt
    71101 gggccaatgc ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcgact gacatgcagt
    71161 tgattacaca aatccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg
    71221 gcgcgggaaa gcaaccgacg gtaactctga tgactgatgc tttccgccgt caccattggt
    71281 tgcgcttctt ccagcatcgc ccccatcgcc attagcaaat tatcccccag cacttttcgc
    71341 actgcaggct gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca
    71401 aagccccaca gcgcggcttt atgctgctct ttcacttcca gcgccgactg gctacgcagc
    71461 atatgtaata cccgatccgg attgtgcaaa aagttagcct gacaggtgat gacatcttca
    71521 gacagcacta cgcccaggat cgtataatcg tccggcgtac ttaattcaaa ctcggttcca
    71581 ccggggcgcg tcgctatttc tgcgcttccc agacattgcg aaccgataaa tccctgctca
    71641 ccgcgcgtcg ccggaatgcc aaaccagaac gagttcggcc agaccaggca cgactgacgc
    71701 agcgccagac cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat
    71761 tcaccatgaa acttgcccgg atgcagctga tcgtaaatct gctgctaggc ggtaatcgtc
    71821 aaagcatgtt catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc
    71881 ggcgtgagct tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta
    71941 cgggtctttt tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt
    72001 taatggggct tgagacgata acgactactg cgtttacgta acgtcccggc agaaaagagc
    72061 tgttcgagcg catttctggc tttttccagc ggccagccaa agtgggcggc cacttctcct
    72121 gccgtcattc cctgacatac tgatgttaac agcgccaaca actcgtcagc agattccgcg
    72181 acgataactg gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt aaagccagta
    72241 accagccact gggtgtcatc gtctggccga ccgatctcct tacggctgat gacacgcccg
    72301 gtacgcatcg cggcagcaca acctgcgtcc agcgccgcac gacacgccgc cagatcgcct
    72361 tccaccacca gcgttaagcg accagggtca agcacttcgt gactgagcag acgaacgtta
    72421 gctgctttga gcatggcatc cgctgcatct atcgcggcga ccatgccgtc cacttccagc
    72481 aatcccagtg cattgatcat cggcaacctc cgttacgcac gctggattgg attacgcgcg
    72541 atttccagca ctgcatcggt aaaggcgtta caggctgctt tacacgcggc ctggctaccg
    72601 gttaaaaatg ctgccgagta gttggtttca gacggtggcg ggacgtaggt caccagctgt
    72661 acgtcggcag atttcaacgc ggcatcaatg ccgtaggtcg cttccagcgg cggtgccacc
    72721 agatacgcca tcggatcgcc aagcgtgatc ccggcggttg atgaaagata agaaccggta
    72781 cgcgaaacca catgcgccag gaaggcggtg ttttctgcgt cattggccca ctggaaagct
    72841 gcgccatttt caatatgtgc aaccatcgca tccagaccgg cacgcacttc cgccgggttt
    72901 ggcccaccaa gcataatcag cacttcaccg gcagtcggtg acgggccgtg tgccgctccc
    72961 gcatacagcg agcgaccata taccacttcg accatcgctt gtttggtcgc ttcgtcagcc
    73021 gcaatgtagg ttacgtcgtc agaatcggca gaaatcagcc cgaggctacg aatatgcggc
    73081 ggcaatttta gttcacgcgc aaattcagcg ttaacggagg caatcacccg catggcggtt
    73141 accgacggtc gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg
    73201 ccggatgctt tctgctccag catacgtttc gccaaatcca caatgacggc acagcagcac
    73261 gaaagagaag caaaagtcag ctacctggta cactaggcag cgggtcagca aaggggggca
    73321 aatgcccctt ttttcatgtc agataatcag atcattacct caataaacta ataatcacat
    73381 cgcggttaca accagtttga tagtgatagc tcaaacccag acgtggcgct ggctgcaagc
    73441 aaaataagcc gcaaaaatgc gaccccatgt ttactagccg caaaaatgcg gtcctatttg
    73501 ccagaacctt ttattattca attggttagt gcaagaaaaa gcgtgattac tgttcaagaa
    73561 gtatccagaa aggtcatgat tttgacgctg aaaaaaaata ggtgggcatt tttgcccact
    73621 cactagccgc aaatttgccc actcactagc cgcatttttg cggcccaaag taataaatac
    73681 agagaaataa taaatacaga tcagcgccaa gatttttcgt cttttcggcg gaatcatgag
    73741 actagtgctt acgtcctgat aacctccaga gcagacctta acttctgtgc agcaggacta
    73801 cgctcattgc cgctcggcta ggtcaaatcc tcacgataaa gctgttcgga ttgtgaccat
    73861 aatgttaaag gactctttaa atcattcctt gacgaattaa ctagctggct taagcggaat
    73921 agcatgcagg ataaaaacaa aaccagctta gcagtgggga gagcttcgct tcgctcgtct
    73981 cctggggcca atgctcaatg aaacgacgta aggcacggag cgctcaccat aggtagacac
    74041 ataaacatct gagagcgcac cttctatcgc gctacgcgct caataaaaac caacgacacc
    74101 gccacactgc aagaccaatg gtttttatcg cgtatacaga ccagacagga aacagcagca
    74161 aagcagcgta tagccaggtg cagacagcac actcacacga cgcacagcag gcttaagtga
    74221 tcactcagga aactttatcc ccgccgccga tcaccgcgaa ctatcatgac cgcgccaaat
    74281 tcgaaaggaa tggcccatag aacgcgatga acacacataa gcgctacact tacttaacgt
    74341 cgccacagag cgattgagag cgcttaaaat cgatgcgatt tcattaaatt tttctcactc
    74401 gcctgaatgg tggcgatcag gactgatcgc aacattgccc cgcctcgata ttgagcgcat
    74461 aatagataca tacattaagc ggaaaagaga taagtttatt gtgaaaatca gggggttaca
    74521 tatcatgcgc atatcagaac aacaacggca gatcctcgac tatctggcaa aaaacgaatc
    74581 aatcaccgca cgacaagccg cagatctcgt ttatggtggc aatgttaccc gcgtccaggt
    74641 tgacgccgcc cgccgctcac tcatgacgat ggttagtaat gggctaatca ggaaacaagg
    74701 ccgcgctttc gtagctgaaa gacatatcaa tcacgcttat agccaggacg ttgaagaatt
    74761 gcgccgccag atcctttggg tattcagcaa acagggtcag gagtatttcc tttctgaaca
    74821 cccaaatttc gccgccaaag agttaacagt aagcggattg tcgtgcgcac tggattatct
    74881 tgtcggtgaa ggttgcgtta cttacaagca gctatggcac gccctgaata cgcttgtaga
    74941 agatggttta ctcattgcca cgcgtgagaa catcgaatac tggataatgc cgcgttcagg
    75001 tcgccgcaat catgcacggt atatttcagg caacagatac agaacgccgg actttaatcc
    75061 gacacaggaa gatcgggacc gtgccgaaat gatgtgcagg gaacaggaga aaagaattat
    75121 ggataagtta ttttcgatgc agtgaacaca agcgggcgca atgcccgttt ttattgcctt
    75181 attccacgcc tgacgaatca atttgatcac acctgagaaa atgcatagcc agaagttaag
    75241 gccgttatat tgcgattggg agcgttttaa acggtatcaa tttaattaat tctgatgtgg
    75301 tgatttgcag gcaaaaaaaa cccgccggag aagcgggaaa tagagacata tcgttgtagt
    75361 tattaacaga tcactttgag gtcgagggcg gctaaccctc gctaatatta tcccgctatt
    75421 accttgcggg tgcaaataat cttttattgc tttgatgatt taataaatag cactttttgc
    75481 taacaaaaac tgctgtcaat atatggccaa aaatgtcaac agaaaaaata aggtttataa
    75541 tattaagtat cacatgggta atgggttcag ggaatcggaa tccaccgcct cacggaaatt
    75601 caaaatttgc ttcttctttc ctccgtgggc ggcttctttt tcgtatcgag gtatttatga
    75661 aagcaataac attagaacag ttcgcagcaa tggcgaatgt atcgaccaga acgctgaggc
    75721 gctggctaaa aaaaccatca accggagtcc ctttgccatt taccccacgc ggtactcgcc
    75781 aattattctg ggagcacgaa tgcaaagaat ggttgtacag aacagaacga taataatcac
    75841 gcatcacatt ttgaggtaaa catcatgacc aatatcgaaa gattagaacg ctgtgccgat
    75901 attatgcggc gcagatggat ttatgaccct gaagcgggtt tattgtttag ccgtgaaaca
    75961 aaagacgtta tcaaaggcag catgaccaat aaagggcatt tggttgtaac ggtacatgaa
    76021 ggtaaattca aagtgacact cacctatcag aaagcctgct atgtatacac atacggcgct
    76081 tatgacgaga cattatatga agtgcaccac gttaatttga acaaacagga taaccgcatc
    76141 aagaatattc gcctgatgcc gaaagaaaag caccgccgga tacataaaaa cgtccgcaaa
    76201 cttattcggc tgggccacac acaatggctt caaccgcaat taattgaaca atcactataa
    76261 acacaggagt aaataccatg cattcattag acagttattt tcagcgcacg acagccccta
    76321 aatcagccgc ccaggaacgc cgtgaggaat tccatgagaa agtgatgcgt tctgctgatt
    76381 atattgcgga caagttcgtg gaaacggttc gtccacttgt tgatgaagta gctgataaac
    76441 ttcagtctga aatgccggaa gatatggaag gcactgcaaa gcggcgactc atttgcgaat
    76501 taagtcgtcg ttttggcgtg tcaataagcg cttttaaata atcgaaaaaa ggggggaaag
    76561 ataatggacg catttgaggt actggcaaat aaaggcgctg aactggtagc acagcgtgat
    76621 aaaaccgcca acgaagggga acgcgcactg ctaaataaac agattaaagc gatccgtatg
    76681 gcccagttta aattaatcag taatgaagtt atcgaaacaa ttaaaccgca gatcccacaa
    76741 atcgttgctg acgctattaa agccgccgga ctggagaaac gcattgccgg gctaaaaacg
    76801 aacgcagaga aaatggagat gcacaaacag agcggtgctc cgaatcccga tgagtacttc
    76861 tcaccggatg ttgagtttat ggcgcaggtt gaggaacgtc tgggtagctg tctcaccgag
    76921 gaacagcgtc gctattttga tggagtggat agttcggcag gtattgacct gaactcgtat
    76981 ttcggtcgtg aaattgagca ttttgatgcc gccccgtcta tgccggagcc agaaccagaa
    77041 atggccaccg atcctgaagc tgagcaacgg aaacgcgtat tcaatgcgat agggattcgg
    77101 tactgatcgg ataaaagaaa gccgggaatt accccggctg acttaacaat gtgaccaatg
    77161 aacaaatcgg acaagacaaa caggataagc agtctcgaaa caatgttcgg gaactatatt
    77221 agcacaagtc cggagaaaat catcatgttg gcactacgca tcatgcaggg catcgctaag
    77281 accctcgcag aacatgtctt agacctgaaa cattcgccac tgagcaaaca ggccatgaag
    77341 cgccaaacgc ttcgtctgtg ggctgaatat tcgcttggaa ctatcaataa aatcatcgac
    77401 atgaaatcgg ggccgagcaa ccagagcgcg gaggaaatgg agttcatcag gcgtttaatt
    77461 ctcattcgca gagacattca tagccagtta cacagcgtgg gaatcgatat taatgatggg
    77521 acgggcgatt aattttcgga caagtactat gcgcgcgcga ggagctgaac atgaagcaag
    77581 atattgcaga caggctggag atactcgaag gccagcgggc agaagcgaaa caactacgta
    77641 agcaggccag aagagcgcac aggaacaacg aagcagagtt actaaccaaa tacatcagtt
    77701 tcaccaatta ctgcatctac gagtgttaca aggaagatgc agaggactgg ctggattcat
    77761 tgccagaaca gtactgagca gaataacacc acgtcatgtt gttctgaggt gagcatatca
    77821 cctctgttca ggtggccaaa tttagtgtgc cacttcagca tcaccgttga aatcacaata
    77881 acgatttttt tgtaaatatg ctctcacata tgtaggaata gaccaggttg gcattgaaac
    77941 acatctacca tgaccataat aaagtaattt tttgatatgt ttatggtttt atattaatta
    78001 aaatatatct aaaattgata gttttaaaat tgcaaaatcc agattgtgtt tgtaatgttt
    78061 aatctgattg tattgagtgg cgtttattat gaaaaataaa tcaggtaatt cttgttatag
    78121 agaagcagtc gatattcata gctcacttga gctttcattt atattgcata aatatcattt
    78181 gcgtgtagat gagaagaaat ctcgaaatgt attcagcgcc atacgtgatg agctcatcct
    78241 tgatggggat tcacgacaaa acttagctac tttctgtcaa acatggcttg atgatgaaat
    78301 ccgtgagttg cttaatttag ctattgataa aaacatgatt tataaagatg aatatccaca
    78361 aatggcggaa cctgaaggtc gctgtgtgcg tatgctttcc gattcgtgga gtttccccga
    78421 ttcgcgacac accttaggtt gctcaaccat tggttcatct gaagctgcta tgctcggccg
    78481 cttggcgtta aaatggcagt ggtgtaaaaa acgcgaagta caaggtaaat caaccgaacc
    78541 tgatttgcga tcctgtgcaa atatgctggc ataaattctg ccgttactgt gatgtgaaat
    78601 tgtgtgaaat tccacttgat gatcatcacc tgataatgaa tgctaaagag gttttgaagc
    78661 atgtcgatga aaacactatt ggtgtcgtta cgactttagg tattacttat ttttgtaaag
    78721 cagattgcgt tagtgttttt aagataagtg ttaaagttaa tattagtttt tatatttaca
    78781 tcgtaattat atgtttctat tttaaaaatg ttcgagagta taaaaatgtc taatgctaat
    78841 atcgcaccca aaagtaagaa aatgacagtg gggacactcg ctatcatgaa tataactgct
    78901 gtagtaagtc tacgatttat accttctgaa gcagaatacg gcttaggtgc cattttttac
    78961 tatacctttg ctgcaattat gtttttagta cctgttgcct ttgttgcggc tgagctagct
    79021 acaacatatc cacaaaaagg gggggcattt cgatggatag gagaagcatt tggggctcgc
    79081 tggggttttg taggaatgct tatgacctgg ctacaagtca tcccctactt tccaacagta
    79141 ctgacatttg gtgcagtctc ggttgcattt attgatgttg atatgggggt ggcagaatca
    79201 attgccagta ataaatggta tattttattt ttcgtactat ttgtttactg gggagctatt
    79261 tgtgttgcat tacgtggcgt tggtattttc tcgcaggtat ctaagtggtg cggtattata
    79321 gggacaatta ttccagctat tatccttgtt attcttggtt tctcctatct gtggttcagc
    79381 ggtaaaccag cggtgatccc attaggttgg ggtgatcttt tccctaatat agctaacttc
    79441 cagaatattg ttcttgccgc cagcatattt cttgcttatg cagggatgga aatgaatgcg
    79501 gtccatgtta acgatttaga taatccaact aaaaaatacc caattgcaat taccatcgct
    79561 tctataggta ctattgcaat attcctgctc agtactttag gggtcgcctt tattattcct
    79621 acagataaaa ttagtctaac tcaaagtttg ttggttgcct acgatatgct gttcgaatgg
    79681 gctggagtgc catggcttgg ctctgtgatg gcatttatgc tagcaattgg cgtactgggt
    79741 ggggtggtta cttggattgc agggcctaat acaggtgtat tagcgatcgc gaaagctggt
    79801 tatttaccta agtttttcca aaaaaccaat cgtcatggaa tggggcacca tctaatgttt
    79861 gtacaaggta taattgtctc agttctttct gttacttttg tgataatgcc ttcagttcaa
    79921 gcagcttttc agattttatc acagttaaca gttatgctct atttagttat gtatatgctc
    79981 atgttcgcta gtgctatcta tctacgacat tctcaacctg atacaaaacg cccatatcga
    80041 gcccctgctt tatttttctg ggcaacactt ggattcttag ggtcttttct ggcttttgta
    80101 ttaagtttta ttccgcccga gcaaatccca actggtagtc caaccagcta tgttctttat
    80161 ttgttagtat tagttgccct atttttatta attccaatat taatttacgc cttccgaaaa
    80221 tctgactgga aagatccaaa ctctgatttc gaaccatttt cttgggaaaa aagaactagt
    80281 aattaaattt tccataagct acaaaagatg aggttaaata tgtttagtgc tgaatctcca
    80341 attggtgtaa tagactcagg tgtaggtgga ttgacatcgg taaaggaaat aattaatttg
    80401 cttcctggtg aagatattat ctattgtgga gataatatga atgcacctta tggcaaccgt
    80461 tctgccgatg atatcatcag tttgactaaa aaaatgctga cttttttaca gtcaaggaat
    80521 gtcaaactag ttgctgtcgc atgtaatact atatcctcaa ctttggaatc cgaagaatac
    80581 agcggatatg ctaagagctt tccgtttcct atcttatcca tcattgaacc tgcagtagaa
    80641 gatgtgattc gccagcaata taaaaatgtt ggtattatcg ctactgagtt tactattaaa
    80701 acggggtgtc acaaagagct aataaaaaaa ttgaattcaa caataaatgt atttggagaa
    80761 ccaagcaaaa atcttgcaat gttaattgag gaaggtaatc ttaatgctcc tgcaatcttg
    80821 aatgacatca aaaaacatgt taaccattta atttccctcc atccagtaaa tgaaattatt
    80881 ctcggctgta ctcactatcc aattgttcag aatttttttg aagcagttgc accggatata
    80941 aaatttatta atcctgcaca tgatcaggct atctctatta aaaatcactt gggacagcta
    81001 aatttattaa actcctcaaa tataggaact ttgcaaataa atactagtgg aagtatggag
    81061 atctacaaaa cggtactagg ggaattgagc attacgaaac ctcatacatt cagtatcagg
    81121 cagttttaaa tttatccggt gcaccgtaaa atcccgtcct tctgaactgc accccaaaag
    81181 ttggacacca actgagtaag gtgcatttta tggacaaacc cagttacata ctgggtagtg
    81241 catccaatta gtagaacatg tgtttttcga taaacgctcc gatcactttt tcgtggatct
    81301 caactgagcg tgagaagcag attgttttac gacccaaccg cttaatgcgg gtgcgtagcg
    81361 tcagattatt acgctcaatg cgttgggtga atattttgcc ggtcagatgc ttattcttcg
    81421 gcacctcccg gccatagctg ccccagtcat cgctggtcag catgccgatg ttgaagggtg
    81481 taagcagtgc cagtagctcc cggcacgttt gatcggttcg gggaccaaaa gtgtaggcca
    81541 gtacaccgcc tgttttggtg ttgtacgcgt acccgagcca atgttgccgg gctttactgc
    81601 caacgtagct ccattgctca tcaagctcgc agataaacgc cacatcagca tgggcaacag
    81661 gcgaagaagt tattcgcttt ggcgcgagtt ttttaaagtc cggatgacgg tgttactacc
    81721 aattttcagt gtcctggcgg tatcgcgaac cccggcacca ttaaaggcca tttcagttat
    81781 cagctctttc atacccggct tacgtgcttg ataagtataa gtgaactgaa acacacggtg
    81841 gcagtcacgg cagcgaagtc tgtcacggcc tttagggttc tgaccatggc ggtaaacctg
    81901 agctgactga caacggggac aatgaatatt aacgctggtc atgagataac ctcaaaagcc
    81961 cgtattatac atcagattca actaattaga ggcatcaccc atccagcatt ataaaacata
    82021 ctaacaataa ctaaattata ataatcacaa cttacaatca aataataaat gcataagatt
    82081 aacaatgatt ttttcgggtt atatttatct aaaaaagcag aatattatgt aacttattat
    82141 tttctaagcg aaaatttatt gtgcggtcag actaacaaac ttgcgtacca cccgaagaac
    82201 atgcgaagat ttaagtattc tttctttttt ggtttttatt tttcattttc tcttttcttc
    82261 tctttccggt tctgttctgg ttttggtttt ttccttttta tttttattct ttcttcaggg
    82321 ttatgctaaa acaccaaaac atttttcacc acaaaagcat ccaatcatct tgccagacca
    82381 atcaccttaa ccggatacac tgccggatat aatcccacta acacccagac accaagcacc
    82441 atacctgata actaccgtat tcaggaagga aatgcgagtg atttcaccgt caccggcaca
    82501 accgatccgc caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg gacaatcact
    82561 tcgtcaatga ctgccaacgg gagttgttgt agctgtgggc gcaacaacat gcccagagct
    82621 ttaccaaagt cctgcccggc caccaccagc aggggatgcg gattcggaaa acgcgcgacg
    82681 aaatcgacca gcgcgttgat gaccgtcaga accgcggcgt aacgcacagg cagcgaggcg
    82741 ggaagcgcca gcacgtacac gtcagttttg ggatcaagat ccagctgaag cagcgcctgt
    82801 tgccaggcac tcaccagatc cgtttcatca atcgggatcg ccaccggcaa attgcgcagc
    82861 ggcagttgta cgccctccag ccagattgtg ctgccagaga gcgaaagggt atgtgcaccc
    82921 gcgccaatca ccgtggagcg tacggtttgc gccggaaact gcacattcat ctcacgcagg
    82981 cgcggatggt catgcagcgc cgttgccaga agtgggccaa tatcggcaaa acagaacggg
    83041 ttggcgggct ggtggcgata acattcgccc acgcctccag aaagcgtaat gatttcgggc
    83101 gtaacacctg cgggcagcaa accggtttgc atcaatgcct gcgcgagcgg cgaaagcgtt
    83161 ccgtcaatca cttcgacaat cagcgccgcc atccgccggg taacctgcac cagctgcgcg
    83221 ccggtcagcg aacgggcgtc agtgcctgca ccgaagcact catccacaat catctgcccc
    83281 ggtttatgag cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg
    83341 ttgagacagg cagtgccgct gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca
    83401 ccgccgatat cgatattcag tacccgacac agccgttgtt cagaaagggt ttgcgccccg
    83461 gctccgtgac cggcgatcac ggattcgagg tgcggcccgg cgctggcgac gacaaaatcg
    83521 cccagcgact gtttttcctt catcactttt tccatcagct gtgcgaccag gctttcggta
    83581 aactggcctt ccggcagctg ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg
    83641 tcggctggca cgctggcggg cacggacgcc gttaccggga tcgattcatc acattcggtg
    83701 atggtaaaac ccagtagatc cgccacttca cgggcttccg gggtaataat gctggcgcgc
    83761 agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg
    83821 atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg
    83881 ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga aacccgtctc
    83941 aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac
    84001 ggcgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg
    84061 gtcatttgcg ccatggacat agatcagcat gtctacatcc tgtagcgtgg taattaaggc
    84121 gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc ccttatcatt
    84181 aaattccacg gccagtgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa
    84241 cgttgttttt cctgccccga cgctacccac aaacgcaatt cgtttcatga atgc
//
LOCUS       DAEEDS010000003        78634 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEEDS010000003 DAEEDS010000000
VERSION     DAEEDS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78634
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.003"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            499..1114
                     /locus_tag="KLH32_000191"
                     /pseudo
     CDS             499..1114
                     /locus_tag="KLH32_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1217..1540
                     /locus_tag="KLH32_000192"
     CDS             1217..1540
                     /locus_tag="KLH32_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBE0137188.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1607..3064)
                     /gene="nuoN"
                     /locus_tag="KLH32_000193"
     CDS             complement(1607..3064)
                     /gene="nuoN"
                     /locus_tag="KLH32_000193"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBE0137189.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3071..4600)
                     /gene="nuoM"
                     /locus_tag="KLH32_000194"
     CDS             complement(3071..4600)
                     /gene="nuoM"
                     /locus_tag="KLH32_000194"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBE0137190.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4764..6605)
                     /gene="nuoL"
                     /locus_tag="KLH32_000195"
     CDS             complement(4764..6605)
                     /gene="nuoL"
                     /locus_tag="KLH32_000195"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBE0137191.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6602..6904)
                     /gene="nuoK"
                     /locus_tag="KLH32_000196"
     CDS             complement(6602..6904)
                     /gene="nuoK"
                     /locus_tag="KLH32_000196"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBE0137192.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6901..7455)
                     /gene="nuoJ"
                     /locus_tag="KLH32_000197"
     CDS             complement(6901..7455)
                     /gene="nuoJ"
                     /locus_tag="KLH32_000197"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBE0137193.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7467..8009)
                     /gene="nuoI"
                     /locus_tag="KLH32_000198"
     CDS             complement(7467..8009)
                     /gene="nuoI"
                     /locus_tag="KLH32_000198"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBE0137194.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8024..9001)
                     /gene="nuoH"
                     /locus_tag="KLH32_000199"
     CDS             complement(8024..9001)
                     /gene="nuoH"
                     /locus_tag="KLH32_000199"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBE0137195.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8998..11724)
                     /gene="nuoG"
                     /locus_tag="KLH32_000200"
     CDS             complement(8998..11724)
                     /gene="nuoG"
                     /locus_tag="KLH32_000200"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBE0137196.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11777..13114)
                     /gene="nuoF"
                     /locus_tag="KLH32_000201"
     CDS             complement(11777..13114)
                     /gene="nuoF"
                     /locus_tag="KLH32_000201"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBE0137197.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13111..13611)
                     /gene="nuoE"
                     /locus_tag="KLH32_000202"
     CDS             complement(13111..13611)
                     /gene="nuoE"
                     /locus_tag="KLH32_000202"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBE0137198.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13614..15416)
                     /gene="nuoC"
                     /locus_tag="KLH32_000203"
     CDS             complement(13614..15416)
                     /gene="nuoC"
                     /locus_tag="KLH32_000203"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBE0137199.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15510..16172)
                     /gene="nuoB"
                     /locus_tag="KLH32_000204"
     CDS             complement(15510..16172)
                     /gene="nuoB"
                     /locus_tag="KLH32_000204"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBE0137200.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16188..16631)
                     /gene="nuoA"
                     /locus_tag="KLH32_000205"
     CDS             complement(16188..16631)
                     /gene="nuoA"
                     /locus_tag="KLH32_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBE0137201.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17262..18200)
                     /gene="lrhA"
                     /locus_tag="KLH32_000206"
     CDS             complement(17262..18200)
                     /gene="lrhA"
                     /locus_tag="KLH32_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBE0137202.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19120..20337
                     /gene="alaA"
                     /locus_tag="KLH32_000207"
     CDS             19120..20337
                     /gene="alaA"
                     /locus_tag="KLH32_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBE0137203.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20421..21020
                     /gene="yfbR"
                     /locus_tag="KLH32_000208"
     CDS             20421..21020
                     /gene="yfbR"
                     /locus_tag="KLH32_000208"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBE0137204.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21079..22911)
                     /locus_tag="KLH32_000209"
     CDS             complement(21079..22911)
                     /locus_tag="KLH32_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBE0137205.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22998..23648)
                     /gene="hxpA"
                     /locus_tag="KLH32_000210"
     CDS             complement(22998..23648)
                     /gene="hxpA"
                     /locus_tag="KLH32_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBE0137206.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23659..24153)
                     /locus_tag="KLH32_000211"
     CDS             complement(23659..24153)
                     /locus_tag="KLH32_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBE0137207.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24236..24691)
                     /locus_tag="KLH32_000212"
     CDS             complement(24236..24691)
                     /locus_tag="KLH32_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBE0137208.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25029..26231
                     /gene="ackA"
                     /locus_tag="KLH32_000213"
     CDS             25029..26231
                     /gene="ackA"
                     /locus_tag="KLH32_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBE0137209.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26306..28450
                     /gene="pta"
                     /locus_tag="KLH32_000214"
     CDS             26306..28450
                     /gene="pta"
                     /locus_tag="KLH32_000214"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBE0137210.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28640..30160
                     /gene="yfcC"
                     /locus_tag="KLH32_000215"
     CDS             28640..30160
                     /gene="yfcC"
                     /locus_tag="KLH32_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBE0137211.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30193..30735)
                     /gene="yfcD"
                     /locus_tag="KLH32_000216"
     CDS             complement(30193..30735)
                     /gene="yfcD"
                     /locus_tag="KLH32_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBE0137212.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30793..31344)
                     /gene="yfcE"
                     /locus_tag="KLH32_000217"
     CDS             complement(30793..31344)
                     /gene="yfcE"
                     /locus_tag="KLH32_000217"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBE0137213.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31400..32044)
                     /gene="yfcF"
                     /locus_tag="KLH32_000218"
     CDS             complement(31400..32044)
                     /gene="yfcF"
                     /locus_tag="KLH32_000218"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBE0137214.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32180..32827
                     /gene="yfcG"
                     /locus_tag="KLH32_000219"
     CDS             32180..32827
                     /gene="yfcG"
                     /locus_tag="KLH32_000219"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBE0137215.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32884..33246
                     /gene="folX"
                     /locus_tag="KLH32_000220"
     CDS             32884..33246
                     /gene="folX"
                     /locus_tag="KLH32_000220"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBE0137216.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33267..34160
                     /locus_tag="KLH32_000221"
     CDS             33267..34160
                     /locus_tag="KLH32_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBE0137217.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34208..35098)
                     /gene="rpnB"
                     /locus_tag="KLH32_000222"
     CDS             complement(34208..35098)
                     /gene="rpnB"
                     /locus_tag="KLH32_000222"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBE0137218.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35295..36068)
                     /gene="hisP"
                     /locus_tag="KLH32_000223"
     CDS             complement(35295..36068)
                     /gene="hisP"
                     /locus_tag="KLH32_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBE0137219.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36076..36792)
                     /locus_tag="KLH32_000224"
     CDS             complement(36076..36792)
                     /locus_tag="KLH32_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0137220.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36789..37475)
                     /gene="hisQ"
                     /locus_tag="KLH32_000225"
     CDS             complement(36789..37475)
                     /gene="hisQ"
                     /locus_tag="KLH32_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBE0137221.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37565..38347)
                     /gene="hisJ"
                     /locus_tag="KLH32_000226"
     CDS             complement(37565..38347)
                     /gene="hisJ"
                     /locus_tag="KLH32_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBE0137222.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38568..39350)
                     /gene="argT"
                     /locus_tag="KLH32_000227"
     CDS             complement(38568..39350)
                     /gene="argT"
                     /locus_tag="KLH32_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBE0137223.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39616..40185)
                     /gene="ubiX"
                     /locus_tag="KLH32_000228"
     CDS             complement(39616..40185)
                     /gene="ubiX"
                     /locus_tag="KLH32_000228"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBE0137224.1"
                     /translation="MKRLIVGISGASGAIYGVRLLRVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40280..41797)
                     /gene="purF"
                     /locus_tag="KLH32_000229"
     CDS             complement(40280..41797)
                     /gene="purF"
                     /locus_tag="KLH32_000229"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBE0137225.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41834..42322)
                     /gene="cvpA"
                     /locus_tag="KLH32_000230"
     CDS             complement(41834..42322)
                     /gene="cvpA"
                     /locus_tag="KLH32_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBE0137226.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42581..43243)
                     /gene="dedD"
                     /locus_tag="KLH32_000231"
     CDS             complement(42581..43243)
                     /gene="dedD"
                     /locus_tag="KLH32_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBE0137227.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43233..44501)
                     /gene="folC"
                     /locus_tag="KLH32_000232"
     CDS             complement(43233..44501)
                     /gene="folC"
                     /locus_tag="KLH32_000232"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBE0137228.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44571..45485)
                     /gene="accD"
                     /locus_tag="KLH32_000233"
     CDS             complement(44571..45485)
                     /gene="accD"
                     /locus_tag="KLH32_000233"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBE0137229.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45641..46300)
                     /locus_tag="KLH32_000234"
     CDS             complement(45641..46300)
                     /locus_tag="KLH32_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0137230.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46383..47195)
                     /gene="truA"
                     /locus_tag="KLH32_000235"
     CDS             complement(46383..47195)
                     /gene="truA"
                     /locus_tag="KLH32_000235"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBE0137231.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47195..48208)
                     /locus_tag="KLH32_000236"
     CDS             complement(47195..48208)
                     /locus_tag="KLH32_000236"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE0137232.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48274..49410)
                     /gene="pdxB"
                     /locus_tag="KLH32_000237"
     CDS             complement(48274..49410)
                     /gene="pdxB"
                     /locus_tag="KLH32_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBE0137233.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49509..50504
                     /gene="flk"
                     /locus_tag="KLH32_000238"
     CDS             49509..50504
                     /gene="flk"
                     /locus_tag="KLH32_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBE0137234.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50501..51679)
                     /locus_tag="KLH32_000239"
     CDS             complement(50501..51679)
                     /locus_tag="KLH32_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0137235.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51934..53175)
                     /gene="fabB"
                     /locus_tag="KLH32_000240"
     CDS             complement(51934..53175)
                     /gene="fabB"
                     /locus_tag="KLH32_000240"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBE0137236.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53334..55340
                     /gene="mnmC"
                     /locus_tag="KLH32_000241"
     CDS             53334..55340
                     /gene="mnmC"
                     /locus_tag="KLH32_000241"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBE0137237.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55461..55739)
                     /locus_tag="KLH32_000242"
     CDS             complement(55461..55739)
                     /locus_tag="KLH32_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBE0137238.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55773..56321)
                     /gene="epmC"
                     /locus_tag="KLH32_000243"
     CDS             complement(55773..56321)
                     /gene="epmC"
                     /locus_tag="KLH32_000243"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBE0137239.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56321..57130)
                     /locus_tag="KLH32_000244"
     CDS             complement(56321..57130)
                     /locus_tag="KLH32_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBE0137240.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57130..57954)
                     /gene="mepA"
                     /locus_tag="KLH32_000245"
     CDS             complement(57130..57954)
                     /gene="mepA"
                     /locus_tag="KLH32_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBE0137241.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57958..59043)
                     /gene="aroC"
                     /locus_tag="KLH32_000246"
     CDS             complement(57958..59043)
                     /gene="aroC"
                     /locus_tag="KLH32_000246"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBE0137242.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59078..60010)
                     /gene="prmB"
                     /locus_tag="KLH32_000247"
     CDS             complement(59078..60010)
                     /gene="prmB"
                     /locus_tag="KLH32_000247"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE0137243.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60176..60727
                     /gene="smrB"
                     /locus_tag="KLH32_000248"
     CDS             60176..60727
                     /gene="smrB"
                     /locus_tag="KLH32_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBE0137244.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60831..61721)
                     /locus_tag="KLH32_000249"
     CDS             complement(60831..61721)
                     /locus_tag="KLH32_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBE0137245.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61708..62232)
                     /locus_tag="KLH32_000250"
     CDS             complement(61708..62232)
                     /locus_tag="KLH32_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0137246.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62229..62699)
                     /locus_tag="KLH32_000251"
     CDS             complement(62229..62699)
                     /locus_tag="KLH32_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0137247.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62696..63244)
                     /locus_tag="KLH32_000252"
     CDS             complement(62696..63244)
                     /locus_tag="KLH32_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0137248.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63249..63971)
                     /locus_tag="KLH32_000253"
                     /pseudo
     CDS             complement(63249..63971)
                     /locus_tag="KLH32_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63991..64713)
                     /locus_tag="KLH32_000254"
     CDS             complement(63991..64713)
                     /locus_tag="KLH32_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0137249.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64773..66005)
                     /locus_tag="KLH32_000255"
     CDS             complement(64773..66005)
                     /locus_tag="KLH32_000255"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0137250.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66054..66632)
                     /locus_tag="KLH32_000256"
                     /pseudo
     CDS             complement(66054..66632)
                     /locus_tag="KLH32_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66714..67277)
                     /locus_tag="KLH32_000257"
     CDS             complement(66714..67277)
                     /locus_tag="KLH32_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0137251.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(67961..68446)
                     /gene="sixA"
                     /locus_tag="KLH32_000258"
     CDS             complement(67961..68446)
                     /gene="sixA"
                     /locus_tag="KLH32_000258"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBE0137252.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68649..70793)
                     /gene="fadJ"
                     /locus_tag="KLH32_000259"
                     /pseudo
     CDS             complement(68649..70793)
                     /gene="fadJ"
                     /locus_tag="KLH32_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70793..72103)
                     /gene="fadI"
                     /locus_tag="KLH32_000260"
     CDS             complement(70793..72103)
                     /gene="fadI"
                     /locus_tag="KLH32_000260"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBE0137253.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72283..72567)
                     /locus_tag="KLH32_000261"
     CDS             complement(72283..72567)
                     /locus_tag="KLH32_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBE0137254.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72939..74279
                     /gene="fadL"
                     /locus_tag="KLH32_000262"
     CDS             72939..74279
                     /gene="fadL"
                     /locus_tag="KLH32_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBE0137255.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75030..75704
                     /locus_tag="KLH32_000263"
     CDS             75030..75704
                     /locus_tag="KLH32_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137256.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(75886..76641)
                     /gene="mlaA"
                     /locus_tag="KLH32_000264"
     CDS             complement(75886..76641)
                     /gene="mlaA"
                     /locus_tag="KLH32_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBE0137257.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            76935..77867
                     /locus_tag="KLH32_000265"
     CDS             76935..77867
                     /locus_tag="KLH32_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBE0137258.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            77941..78015
                     /locus_tag="KLH32_000266"
     tRNA            77941..78015
                     /locus_tag="KLH32_000266"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77974..77976,aa:Arg,seq:cct)
     gene            78177..>78634
                     /gene="intS"
                     /locus_tag="KLH32_000267"
     CDS             78177..>78634
                     /gene="intS"
                     /locus_tag="KLH32_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBE0137259.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19907 a        21014 c        19300 g        18413 t
ORIGIN      
        1 aatcaatgct tcttatacac aattagaaat aaaacactca caacccaaaa atagagcatc
       61 cggcaaaaga gtgagttaac atcattgtta atcaattcga ccagtcagta aatatgctga
      121 ctggtcgaac ataatttgct aaaaagtcat cactgactga tgtcagtccc accatctgcc
      181 agttcaatct acgggtattt ccagcatatc gcggcacgcg attgctggtc tgttgatgta
      241 gtggattgta gttgctgact tttcttaccc tcagatttct gccccacttg tcaattcaat
      301 ctgccaataa ataacataat tatattttta ttcctcacag aaattccttt ttaaaaatag
      361 cgaacccact gtaaaaaaca ctttttgtat acctctacca agcgtaataa aataaacgct
      421 cataattcat tataaataat aatgaaataa cgccttttat tgataattta catatgaaaa
      481 aaattttcct tttgttatat tatttctgtt ttcactccct ctgattattt ttaccaaccc
      541 tgggtcactg cattatcgcc aacgccccga cacgtaagtc ctgagcaatt agaaaaaaca
      601 gttcgttatc ttacacaaac tgtgcatcca cgtaatgccg gcaatattga taatctgaat
      661 aggtccgcag agtatataaa agagttcttt gtcagtagcg gtgccagagt taccgcgcag
      721 ggcgtccccg attggctatt acgactctgc gcctggaagc cagaattacc cttacccggc
      781 aatgtcctgg ctttatcccg atcggggaga ctttattgct gtggtcggca gaatgcagga
      841 tatcaacgcc gttcgtcagg taaaagcggc attgttgtca tcccgggatt tatctgttta
      901 ttctataaat gccccagggt ttattcccgg tattgatttt tctgatcacc tcaattattg
      961 gcaacacgat attcccgccg taatgattac tgacaccgct ttttatcgta ataaacaata
     1021 ccacttgccc ggtgataccg cagacagatt gaattatcag aaaatggctc aggtggtaga
     1081 tggtgttata accttgttat acaacagtaa ataataaaaa tttctcgagg cgggtccaga
     1141 aacagcgaca tgtacatttt attttccctc gtctgtttta agatgcgaaa cataaaattc
     1201 aatggaggaa aaataaatgg gaatgattgg ctattttgca gaaattgact cagaaaagat
     1261 taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg acacgctttc
     1321 aggattacgc cgactggaat cactggatcg aagtgagctg cgtaaacagt tttccattaa
     1381 gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt tagaggggca
     1441 actctttgcc tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct
     1501 gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcaga gcgccaaaaa
     1561 aaaccgccga acatgtcggc ggctttctga cttacaaact aacagtttac atcagcggca
     1621 ttgctaaccg cacaatacta atcagcggtt gtggccatac acccagcacc agtaccaaca
     1681 gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgac ggtgcatcgc
     1741 gacccggttg ttccggtgcg tgcaggtaca ggctcaccgc cacgcgcagg tagtagtaga
     1801 ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt gcctggacac
     1861 cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata ccggccagag
     1921 acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt
     1981 aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc
     2041 cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc gcttccatcg
     2101 acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag
     2161 atgagtaacc gagcagacgt ttgatgttgg tctggctcaa cgccatcagg ttaccgaaga
     2221 tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg tcacccaccg
     2281 gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc gccgtcgcca
     2341 ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg
     2401 gcaccagaga gagtttgaag ccgaggccaa caatcatcat gccgaaacct gccagcaaca
     2461 gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc
     2521 cagactgcgc atacaccagc gccataccga acagcaggaa agaagacgct gcggcagaaa
     2581 ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca
     2641 ggccaaacag cggcaaagag atcagttcaa taccgaggaa cagagacgcc agatggtttg
     2701 cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag aactcatcct
     2761 tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg aggctcgcca
     2821 acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt
     2881 ccatagcgcc cgcctggcca acaaaccaga gcgaaaccag cgccgcgtta agcccaataa
     2941 ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca
     3001 ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg
     3061 tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc
     3121 caatcgcgga gtgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca
     3181 gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc agttcctggc
     3241 tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa
     3301 cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca acctggaagc
     3361 tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg
     3421 ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca
     3481 tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accgcagaga ataaacagac
     3541 ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc
     3601 tgccggtgta gattgcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca
     3661 gacgtttgat atcggtctga gcgaaggtca tccacgcacc gtagaagatg ccgataacac
     3721 ccagccacat agcgattggc gcgaactctg ccgacgcgtt cgggaacagc ggcagggaga
     3781 aacgcaacaa accgtaagcg gcggttttca gcaagatccc cgcgaggtcc acggaaccgg
     3841 cggtcggagc ctgggagtgc gcatccggca gccagccatg cagcggaacc accggcattt
     3901 tgactgcgaa ggcgatgaag aagcccagca tcaacagata ttccacacca ctggacattg
     3961 gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa
     4021 aaaccagcgc caggatggcg atcaacatca ccagaccact cgcctgggtg taaatgaaga
     4081 acttggttgc cgccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga
     4141 tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga
     4201 tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc
     4261 cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg agcagaccgg
     4321 tcagcacgac catcagcagc gacagaccgt caatggcgag atgaatagag ataccaaaac
     4381 gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca
     4441 gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat cccatggtga
     4501 tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag cacaggaagc
     4561 cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt attccttgta
     4621 aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc tcttcggact
     4681 gggggtagtg tcggatgcgc cgcgaaggca gcttatccga cctacgttct tatatgccca
     4741 ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag caccacgacc
     4801 gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct taacagcaga
     4861 cctttacctg caaagcggga aaaaaccgcc gggatgttca tcattgagtt cagcggatcg
     4921 cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc gtacagccag
     4981 tcaaagcccc aagcgttgta ccaccaggta ctcagcaggc ggcccggcgc actgttggcg
     5041 atggaggtca ccagagaacg tttacccagc cacagccagg ctgccagcag aatgccgacc
     5101 accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc
     5161 gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg
     5221 atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg agcgtgaatt
     5281 tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga ggtcataaac
     5341 gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc acccgcgagg
     5401 atctcatcct tactgaagaa gcccgcagtg accagcggca gtgccgacag tgctgcgccg
     5461 cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc catcttgaag
     5521 atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa cagcagcgct
     5581 ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg cacgccaagc
     5641 gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg tttgatgtcg
     5701 gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac aatacccacc
     5761 agatgcagaa cttccggcgt catcaggaac aggccgtggg tacgggcgat caggtagaca
     5821 cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc
     5881 gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc accgcccagc
     5941 agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt
     6001 tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa
     6061 gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt tcattgctgc tgcgccattc
     6121 ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac gccttcccag
     6181 ccgaggtaca tcagcagcag gttatcggcg agaaccagaa ctaccatgct ggcgatgaac
     6241 aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg
     6301 tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag cgacaggccg
     6361 tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt ccacagcggc
     6421 tggctgtatg cctgctcgcc gttagcgaag aaatcaacgc cgataaaggc ggttaccagc
     6481 gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga ccagcgccca
     6541 cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa ggcaagcatg
     6601 ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga cggtgaagtt
     6661 gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg gcgagaatgt
     6721 acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag gccagcgcgg
     6781 aggcgttaat catgatttcc agaccaatca gcataaacag cagattgcga cggataacca
     6841 gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt tgtaagggga
     6901 tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc
     6961 acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga gcagcagcat
     7021 agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca ctgctttagc
     7081 actgattggc gtaccgtcga taccctgatc gttaacaccg aggatggcgt aaacaatcac
     7141 caccagcatg atggccgaca aaattgccgg gccaatccac acctgcggtt tcagccactg
     7201 gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga acacgaacag
     7261 caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt aagcgcctag
     7321 tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca gtgcgtgtac
     7381 cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc cacagatata
     7441 aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc gataggcttg
     7501 gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc catccggtag
     7561 aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt ctcgtaaacc
     7621 agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat cgccgtggtc
     7681 ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttaat gcggaaaaat
     7741 tccggatacc agcgaccgtc tttggtttct gctttttgca gcgagataca gccgaccggg
     7801 caggctaccg cacagaggtt acaggctacg caacgctctt cgccgtccgg gtcgcgggtc
     7861 agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg gtacattcgc
     7921 gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac ctgggtgccg
     7981 aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc gcctgccaga
     8041 gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag attttccagc
     8101 cgaaggacat tacccggtca taacgcggac gcggtaacga cgcacgaatc aaaatgaaca
     8161 tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac aacgggcctt
     8221 gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc ccgatgtact
     8281 cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa ccatccgcca
     8341 gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga
     8401 tggcaaaggt aataaaacca aagaattgcg ggatgacgtt ccacacatgc gcctggctgt
     8461 tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc agggaaagcc
     8521 cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc agcagcgagt
     8581 atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga cctgccatca
     8641 tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga ctgactggca
     8701 caattgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg gtaaagatga
     8761 cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc atgtccgcaa
     8821 ccagctggag cgaaccgccc cagccaacac ggttaggtcc ataacggttc tggaacagac
     8881 ccagcaggcg acgttcgcca aagctcatga atgccccaca ggtgacaacc accagcagga
     8941 tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat atccaactca
     9001 ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag caatgccgga
     9061 catacccatc ggcaagccca cctgccctgc cgtcagtcct tcggcgattt caaccggcag
     9121 cgtgaccgtg ttgccatcgt aactaaagga gacgcgtgta cctgcgttca cacccaactt
     9181 cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct ggaagaccgg
     9241 agcacgctgt gacaattcat cgctgccaaa caggtggtag tacggcgcga tacgccattt
     9301 cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac cgttttcgct
     9361 ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg
     9421 gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg gcacttgcga
     9481 acggtgcgcg gtcggctggt tgttaccttc catcgagaag gtgaacatgg tgtcaatatc
     9541 ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac gaccgctgta
     9601 acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc
     9661 atctttgata cccgccagtt ccgggatttt cgccacaaca gcgtcaatta catggtcgag
     9721 ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc agcgagtgca gccagcgcca
     9781 gctttccagc atgacagttt tgctgtcgta ataagccgga tcgtaaacct ggaagaaacg
     9841 ttgggcgcga ccttcattat taattaccgt accgtcgctt tcagcaaagc tggcagagga
     9901 aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga tgatcaacca ccatcaccag
     9961 cggcgcttta gccagcgcag cattcacgcg ggtagcagaa gcgtaacgat gcagatcgtt
    10021 ttccaacacc accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg cttcttcaag
    10081 cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa tcatggtgat
    10141 accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag cctgaatcac
    10201 ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac ctgccagtgc
    10261 ctgcacgatg acgtcgattt tgttttgcag ctcaggttcg ataccgtcaa ccgctggtgc
    10321 ggagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt caaccggtgc
    10381 gcggtaagtc cacgccgcga tatcatccag acgagtgtca tcaacgttgg taacaaacag
    10441 cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt cagccacttt
    10501 ctgtgctgca gccatttcgc gcgctttacc tttcaccgcc tgacgcactg ccagtgcgac
    10561 gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt aagattcgat
    10621 ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg ccagttgcag
    10681 acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca ccagttcacg
    10741 cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca ctttcttcga
    10801 ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc
    10861 atcgccacga cgctgtactg gctgacgcgg acgatccttc aggttgacgt aaccgtaacc
    10921 gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat
    10981 acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg aacattgctg
    11041 gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg agtgcgtttt
    11101 gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt cgctttccag
    11161 cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcgccataaa cgcccagatc
    11221 tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga tgcagcggtt
    11281 catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg
    11341 gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc agttaccgcc
    11401 ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga ccacactttc
    11461 acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt
    11521 catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc
    11581 acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt aaggaatatc
    11641 aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct cgtattcttt
    11701 gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcggtca gggaccgagc
    11761 gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc ccattaatca
    11821 aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga
    11881 tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt ttacccgggc
    11941 ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctggcct tcgccacgct
    12001 ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc gtacaccagc
    12061 cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc atgttgatct
    12121 catggtcaac cgccatcgcc agcgccgtac ccagacggct gcccgcttta ccgatacttt
    12181 cgaattccat cggcagatca aggtgcgctt cggtcaggaa gtcagtcccc gcgccgcctg
    12241 gctgccaggc tttaaatttc agaccatcac gcataccacc ggcgtaatct tcgaggatct
    12301 cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg atttttcacc cgaccggaga
    12361 agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac cactccacgc
    12421 cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca caggtcggtt
    12481 tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta gcacgacgcc
    12541 cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct gccccggtat
    12601 ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc aagcccgctt
    12661 cagtggcttc ggcaatggcg cggcgcagat ttactgccgc ttcgatatat tcgccacgca
    12721 ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata ccttccacca
    12781 gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc tccatttcat
    12841 cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc aggctccact
    12901 tcaggccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct tttacctgat
    12961 taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg taaccgtttt
    13021 tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc caggtcagcg
    13081 gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca gttcagggat
    13141 cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc atcatgtttg gccctttatc
    13201 acagttcccc aggcagcaag ttggcagcag cgtaaagcgg ccatcaaatg tcgtttgccc
    13261 tggtttgatg ttcagctttt tctcgagcgc cgcctgaata ccctgatacc cgttgatatg
    13321 acagaccacg ctgtcgcaat aacggatcac atggcgacca accggctggc ggaagatctg
    13381 actgtagaac gttgccacac cttcgacgtc gcttgccgga atacccagca catcggcgat
    13441 cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt tcagcgcttc
    13501 aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc
    13561 tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca taattagcgg
    13621 tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc agacaccagg
    13681 ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt gcggatacga
    13741 gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat ccccttggtc
    13801 gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga cacttgcagg
    13861 aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc
    13921 agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg
    13981 cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca gtcagaaaca
    14041 ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg acgcgcctta
    14101 cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc
    14161 gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat ggtattttgc
    14221 agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc
    14281 agacgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa ccacgccggg
    14341 tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg atcggtaaag
    14401 gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat gtacagcagg
    14461 tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac gcgatccggc
    14521 acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg
    14581 ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg gcgctcgccc
    14641 attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac aatctcttcg
    14701 ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg
    14761 ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc ttccggtttg
    14821 aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa cggcgagaat
    14881 tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt ttgcggcatc
    14941 atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca
    15001 cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt
    15061 tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat cagatggtag
    15121 aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg
    15181 ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa atcgccaact
    15241 tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt cgcctgaaca
    15301 gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc atcaagatga
    15361 tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt caccattgca
    15421 aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag gaataagcga
    15481 agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg caggttggtt
    15541 acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc gcgataaacg
    15601 ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga ttcctgcaac
    15661 agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat atacacatca
    15721 accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat accaccagag
    15781 ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc atacagacgc
    15841 tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga
    15901 cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac
    15961 gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca aattgagttt
    16021 ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt gttaacttct
    16081 tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg
    16141 ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta gcgttgacga
    16201 ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag
    16261 tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa aataaaaatt
    16321 gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca tgcgaacaga
    16381 tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa cttggcggac
    16441 aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac gtttttcgac
    16501 ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca acacaggcca
    16561 atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc agtggatgtt
    16621 gacatactca ttgcttactc atcaaaagta gcgccagatt cactgttctt cacggcagat
    16681 ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaataact gtagaaattc
    16741 ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg tcaactttaa
    16801 caaaagcttc ttcacattag tttacataat atcaacacca ttagcattta atgccctttc
    16861 accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa
    16921 gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct ccccccaaaa
    16981 gagagtattt tcttgatctg tgacacgctt ttgttattcc ataacaaaaa cgcagcaaca
    17041 aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga ttatttgtgc
    17101 attcgggacg tgaatctctg gtgattgaaa aataaacagt tttgtacgtt ctgcactatg
    17161 cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc agcggctcgt
    17221 tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat atccctttca
    17281 atcaacaatg aatcatcccc ttccggagca gacattgggc tgtattgcca cggattatgg
    17341 tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga cgggtcatag
    17401 cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact cagcacacgc
    17461 aggtccgggc tcatcatctc aaccggcctt gccgtcacgc caagaccggc tttcactgcc
    17521 gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccatggaat atctacttta
    17581 ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa tacaagaggg
    17641 atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt tggcgaggta
    17701 cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa atccacttcc
    17761 tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc cagcgctaat
    17821 ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc ggcagattca
    17881 tctgaagcac cgatagttaa cacgccctga agattactga acattaatga tgagcaggcc
    17941 tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc atgttcagtt
    18001 aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg ttccagacgc
    18061 tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc ggcagcaaaa
    18121 gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag gttaattatc
    18181 ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga gctggttaat
    18241 gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca gtttcatccg
    18301 gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact cacattgttc
    18361 cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata tctattaagt
    18421 tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt
    18481 gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag caatggtgaa
    18541 gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa aaccttttat
    18601 ttataagggt cattgcgaat tatctgatgc aaagttatgt tatgttaggc aaagtaatct
    18661 tctattatta ataagcacat caaaaccttt ttgaatatta aataataatt aattagcatc
    18721 atcctcattc attaattccg cttaacaata gtttcacacc ttacccccat tgcgacacct
    18781 cggaaatcat cacgctgtta aactctccgt aatgcggaaa tatcatcatc gcgtttcatt
    18841 agtgaattct taaatgaggc attttcacac aattatctta cagataaaaa aaccagactt
    18901 gcaattaaga attagaacaa ttcactatat aacattgcat gtaaagcata tacacctcat
    18961 tattttgtca ttattaagtt attaacagca caatcgagcc ttcccctctg gcaaaatctt
    19021 attctgcaga ccttcaaaac accgtcctgg gggagtacat tgttctaagc tgacttccac
    19081 ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc
    19141 agcaaattag agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc
    19201 ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc cccattcggt
    19261 tttgacgcgc cagatgaaat cctcgttgac gtgatacgca acctgcctac cgctcaaggg
    19321 tattgcgatt ccaaagggct ttactccgcg cgtaaagcca tcatgcagca ctaccaggct
    19381 cgtggcatgc gtgatgttac cgtggaagat atttacatcg gcaatggtgt atcggagctt
    19441 atcgttcagg caatgcaggc attgctgaac agcggggacg aaatgttggt tcctgcacca
    19501 gattacccac tctggaccgc ggcggtttcg ctttccagcg gtaaagcggt gcattatctt
    19561 tgcgatgaat cctctgactg gttcccggac ctcgatgata ttcgcgctaa aattacgcct
    19621 cgtacgcgtg ggattgttat tatcaaccca aataacccaa ccggcgcggt atattccaaa
    19681 gagcttttaa tggagattgt ggagattgca cgtcagcata atctcattat cttcgccgat
    19741 gaaatttatg acaaaattct ctacgacgat gctgagcatc actcaattgc gccgctggca
    19801 cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc
    19861 cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt
    19921 ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag
    19981 accgcgctgg gtggttatca gagcatcagt gaatttatta cccctggcgg tcgtctttat
    20041 gagcagcgta accgcgcgtg ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa
    20101 cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca aacgctttaa cattcatgac
    20161 gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt gcaagggacg
    20221 gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat
    20281 atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca gctgtaatct
    20341 taatttcact gccggagatt gcatccggca gtgttatccc gccacaatga cctgatgatg
    20401 tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc tcccgcctga
    20461 aactcattaa ccgctggccg ctaatgcgca acgtgcggac ggaaaatgtg tccgaacaca
    20521 gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcgg aaatttggcg
    20581 gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc tcagaagtgc
    20641 tcaccggcga tctccctact ccggtgaaat acttcaatgc gcaaatcgct caggagtaca
    20701 aggctattga aaaaatcgct cagcagaaac tggtcgatat ggttccggaa gaactgcggg
    20761 atatctttgc gccgttaatt gacgagcatg catatagcga tgaagaaaaa tcgctggtga
    20821 aacaggcaga tgcactgtgt gcatatctga aatgtctgga agaactcgcg gccggaaata
    20881 atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg
    20941 agatggacta cttcatggaa gtatttgttc ccagcttcca tctttcgctc gatgagatta
    21001 gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcagtcaat aagcgccgga
    21061 tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca cacaaaccgc
    21121 catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc tgtaatttcc
    21181 cggacctaaa accagggtgt taacaggtga agacaccggt gtcataaagg cggcggatgc
    21241 tgccatcgcc acgaccatcg caaatggata aggcgacact cccatcgttt tggcagcagc
    21301 cagcgcaatc ggagccatca acaccgccgt cgcggtatta gagataaaca gcccaataac
    21361 cgccgacaag acaaacaaac accccagcat catatgtggc ccataaccgc cgccaatatc
    21421 catcagccct ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg
    21481 catcatccca acgatcaaaa taatgctcgg ccagtgaatg gatttatagg cgctttcagc
    21541 atctatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa caggattagg
    21601 aatttcattt gtcagcatta acgccaccat tagcaccaga cagaaaatgg catggggtgc
    21661 ctggctgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg ctacgaagtc
    21721 gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca ggatgatatc
    21781 gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat tgcgcttgag
    21841 ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat
    21901 cagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg cctggtcaga
    21961 aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa attgccggag
    22021 atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa attcagaaac
    22081 cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt
    22141 agcgccataa cgctcacgta atttgagatc atccagccgt tgacctatca ttggcgatcc
    22201 ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga taagatcgcg
    22261 aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa
    22321 acgcatcact aacatataca aaatacccag caccagcacg accaggccaa taggtgttac
    22381 gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga ctaccaggtt
    22441 cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc ccggcaaaac ttaacggcat
    22501 catcagacgt gacggcgaag tttgcatacg catggcaacg cttaacacca cgggaataaa
    22561 gatagcgaca acgctggttg aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa
    22621 caaaaccaac attttgattt cactgttgcc cgccactttg accagccatg ttcccattac
    22681 ggtggcaaca ccggtacgga ccaaaccatc gccaataata aacaaggcgg caatcaggac
    22741 aacgttagga tcagaaaagc cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc
    22801 aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg tcgcaaacaa
    22861 gacgatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca caacttatcc
    22921 ttgttaattg agggggatga cttgattctg ccataaaaaa gccccgacga gacggggcta
    22981 aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt ggtcacagta
    23041 atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt atctgccgga
    23101 gcgttaaccg caatgacatg gcaacccgcc gccaggccag aaagcacgcc agcgggagca
    23161 tcttccacca ccacacactc ctgcggcgca agccccagca gctgcgcacc taacagatac
    23221 gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg ttacaaacac ctctggtgcg
    23281 ggaagccccg ctattttatg gcgcgctcgc gctaccggca tggagccaga agtcacaatg
    23341 gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc aggaagcgcg
    23401 gtaatacctt cggtttccgt ggcctcgatt tgctccagac gcgtaaactc ggcggcaata
    23461 tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg tttaccgtga
    23521 atgaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt gctccacgcc
    23581 cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag aaaacctttg
    23641 caccgcacgc gggcctcctc aggcgttgat aatttgatta atttcgttgg cgctcaaatg
    23701 gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt cccacattgg
    23761 cgtctgggcg ttaaaaccat gagtcccagc gtcaaaatgg gtatagcgcc cttccacatt
    23821 aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa
    23881 ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg aaacatacaa
    23941 cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc
    24001 aaactcgcga tccaattcac gcatctgtaa tccgtaacca cgctcaataa ttgtttgcag
    24061 gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt actgattaga
    24121 caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca ctctactacg
    24181 gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg gtcaattaca
    24241 aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg
    24301 cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc tgcaatttac
    24361 cgcgaacttc ataaaaccag ttaaggattg cagggggtaa tggcgttaca gaacgcttgc
    24421 ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagtgcagtg gcaactgccg
    24481 gcccaagcag accgcccagg gcaatctgcc agcagagagt aaatacggcg atcggcggca
    24541 taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag accggggcaa
    24601 ggcgtttttc cagtggccac gtctttgagt aatgctgtcc gcggcgaaac aagctaaaaa
    24661 aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca tataaagatt
    24721 caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt tgtcttccat
    24781 acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat tcattgagtc
    24841 gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca tagactcaag
    24901 atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt
    24961 gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta tcaattatag
    25021 gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct tcactgaaat
    25081 ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc gaatgtttcc
    25141 acctgcccga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa gcggctttag
    25201 gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt ctggcacaaa
    25261 aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa
    25321 agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa gatgcagctt
    25381 cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct ctgaaatctt
    25441 tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac cagactatgc
    25501 cggaagagtc ttacctctac gccctgccgt acaacctgta caaagagcac ggcatccgtc
    25561 gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca aaaatgctga
    25621 acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt ggttccgttt
    25681 ctgctatccg caacggtaaa tgcgttgaca cctctatggg tctgaccccg ctggaaggtc
    25741 tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac ctgcacgaca
    25801 ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct ggcctgctgg
    25861 gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg acgaaagaag
    25921 acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc ggtgcctaca
    25981 ctgcgctgat ggatggtcgt ctggacgctg ttgcattcac tggtggtatc ggtgaaaatg
    26041 ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt gaagttgatc
    26101 atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa gaaggtaccc
    26161 gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac gcgagccgcc
    26221 tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa cccgccaaat
    26281 cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac
    26341 cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt
    26401 tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca
    26461 gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac cgctgaaaat
    26521 gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg aagagatcgt
    26581 cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc
    26641 gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa cgctgaatgc
    26701 ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg gaacagctga aagagcgtat
    26761 cgaactgacc cgcaacagct tcggtggtgc caaaaacacc aacatcaccg gcgttatcgt
    26821 taacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccggatc tgtccgagat
    26881 tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc
    26941 cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc
    27001 gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca tcaatactcg
    27061 ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg agcacttccg
    27121 tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg ccgcttgcct
    27181 ggcagccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct acgaaatgga
    27241 cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt
    27301 gaacaccaac acctggcaga cctctctgag cctgcagagc ttcaacctgg aagttccggt
    27361 tgacgatcac gaacgtatcg agaaagttca ggaatacgtt gctaactaca tcaacgctga
    27421 ctggatcgaa tctctgactg ccacttctga gcgcagccgt cgtctgtctc cgccagcgtt
    27481 ccgttatcag ctgactgaac tggcgcgcaa agcgggcaaa cgtatcgttc tgccggaagg
    27541 tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta tcgcaacttg
    27601 cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact
    27661 aggtgcaggc attgaaatcg ttgatccaga agtggttcgc gaaagctatg ttggtcgtct
    27721 ggtcgagctg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac agctggaaga
    27781 caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc tggtttccgg
    27841 tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca aaactgcacc
    27901 gggcagctcc ctggtatctt ccgtgttctt catgctgctg ccggaacagg tttacgttta
    27961 cggtgactgt gcgatcaacc cggatccgac cgctgaacag ctggcagaaa tcgcgattca
    28021 gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc tctcctactc
    28081 caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc
    28141 gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg ctgcggtaat
    28201 ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg ctaccgtgtt
    28261 catcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc gttctgccga
    28321 cctgatctcc atcgggccga tgctgcaggg tatgcgcaaa ccggttaacg acctgtcccg
    28381 tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc agtctgcaca
    28441 gcagcagtaa tctcgtcatc atccacagct ttgcgctgcg gatatctgaa ccggaaataa
    28501 tcactatttc cggtttttta ttctcttaat ttgcattaat cctttctgat tatcttgctt
    28561 aactgcgctg catcaatgaa ttgcgccatc tcactttgca tacttaccac tttgttttgt
    28621 gcaagggaat atttgcgcta tgtccgcaat cactgaatcc aaaccaacaa gaagatgggc
    28681 aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagtc ttgccacctg
    28741 ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg atggtcaaac
    28801 aaaaacacgc aaagtcgtag atccacactc atttcgcatt ctgactaacg aagcaggcga
    28861 acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg gcctgatgaa
    28921 cttcccgttt gaagggttaa cctcaggatc gaaatacggg acagccgttg gcatcatcat
    28981 gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa
    29041 cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta ttcctgcgct
    29101 gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg ccgtcgcctt
    29161 tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta ccaccgtcct
    29221 ggtgacttat attgccacgc aaatcggttt tgccagttcg tggatgaacc cgttttgtgt
    29281 ggttgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt
    29341 ggtgtgggtt atcgccactc tgattggcct gatctttacc atggtgtacg cctcacgagt
    29401 gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca
    29461 ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga ttgtcctgac
    29521 agccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta ttccagaaat
    29581 tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg ttttccgcct
    29641 taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcgc gaatgatgat
    29701 cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggttg gtaatggtga
    29761 agcaggtaat gccagcgtgt taaatactat cctcaacagc attgccaatg ccattagcgg
    29821 tctggacaac gcggtcgcgg cctggtttat gttgctcttc caggctgtat ttaatttctt
    29881 cgtgacgtcc ggttctggtc aggcggcatt aactatgccg ttactggcac cgcttggcga
    29941 tctggtcggt gttaaccgtc aggttaccgt gctggcgttc cagtttggtg atggcttcag
    30001 ccacatcatt tacccaacct cagcttcgtt aatggcaacg ctcggtgtgt gccgggtgga
    30061 cttccgtaac tggctgaagg tgggcgcgac cctgcttgga ctgctgttta ttatgtccag
    30121 cgtcgtagtg atcggcgctc agttgatggg ctaccactaa aaatgttaag agccgcaatt
    30181 gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg cattgcgctt
    30241 catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat
    30301 ttcttccggc gtcagccagc aaactccact gacttcatct tcctgtaggg cgaagggacc
    30361 gtgagagacg caactgaaca acgcgcccca gacacggcaa tttttatctt cgaaatagaa
    30421 ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac tcttcttccg cttcgcgacg
    30481 cgcggattcc agcagttgct catcggcctg aactacaccg cctgcggtcg catctaacat
    30541 gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc ccatgccatc
    30601 atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt gttcccggct
    30661 ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag tacttgccaa
    30721 acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg cgcattttga
    30781 gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat cattgagtgc
    30841 gattacacta agtacgtcat tatccagcat gccataaccc gccggattac cgcctttcgg
    30901 aatactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg ccactggcag
    30961 atgggtatga ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc
    31021 aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct gttgccacgg
    31081 cgcggttatc gggaaatgca gcatcatttg atccacttcg ctgtcgcagt tgccacgcac
    31141 agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg gcgcgtaacc
    31201 ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca ccagccactg
    31261 ggcaccgctt tggacaaaca actccacaac acgttccgtc gccggtaacg acccatgaat
    31321 gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta agctatgata
    31381 ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata aaacgctgga
    31441 cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcgttctggc acctcatcgc
    31501 catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg caccattcac
    31561 caaataaatt cggctgaccc agtgctaaca aatgttctgc catcgcgaac agtttctctg
    31621 cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg
    31681 tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg
    31741 cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc
    31801 gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta
    31861 ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc
    31921 tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccagg
    31981 cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac
    32041 tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct ttttgtcact
    32101 gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga
    32161 ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa
    32221 aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacctggg
    32281 gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc
    32341 aattgttgat cattctcctg ccgatggcgg cgaaccgtta agcctctttg agtctggtgc
    32401 cattttgttg tatctggctg agaaaacagg actctttttg agtcatgaaa cgcgtgagcg
    32461 cgccgccaca ttacagtggt tattctggca ggtaggcgga ctggggccga tgcttgggca
    32521 aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca
    32581 ggttgaaact cagcgtcttt accatgtact aaacaagcgg ctggaaaact cgccctggct
    32641 gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac
    32701 tcgcctgcga attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg
    32761 ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg agcgttcgga
    32821 tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct
    32881 gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttgcg tacgtttatc
    32941 ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac
    33001 taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc
    33061 gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgct ggaaaaatta
    33121 actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag
    33181 atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag
    33241 cgttaatcgc catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa
    33301 ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaattacg gtagtgacgc
    33361 gtaacccgca gaaagccagt tccgttctcg gccctcgggt gacactatgg caagggcttg
    33421 ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg
    33481 ctgataaacg ctggactcac aagcaaaaag agcgtctctg ccaaagccgc tggaatatca
    33541 cgcaaaaact ggtcgatttg attaatgcta gcgacacgcc gccgtcggta ctcatttccg
    33601 gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc
    33661 cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc
    33721 aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg
    33781 gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attggttccg
    33841 gtcggcagta tctggcctgg attcatattg atgatatggt caacggcatt ctctggctgc
    33901 tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccggta cgcaatgaac
    33961 aatttgccca tgcgctcggt catgcgctgc atcgcccggc tattttgcgc gtccccacaa
    34021 ccgccattcg gctgttaatg ggcgaatctt cagtactggt attaggtgga caacgcgcgc
    34081 tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc
    34141 tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa ctccctgtgt
    34201 tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc accatcataa
    34261 ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgt
    34321 gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct
    34381 tttcttgtgg tgcgcgttct gttatctcac caataaatgc acggaaacgc tgggcatccc
    34441 ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa
    34501 ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct
    34561 gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc ggcaccacgg
    34621 taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca ggctcggcaa
    34681 attcatcaag ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca
    34741 tcgggatcac caatggaagc tctttatagc ccgcatcaag atggttttgc attgccgcaa
    34801 tggaataacg catcatgcga aaagccatta attcttccgg cttactttgg tgctctatca
    34861 ctacataaat ataacccact ccctcctgcg ttttcacaga ccacaagagg tcggaataat
    34921 attgccgcag gtcttcatca ataaaactgt ttggttctag tttaagcgtc gttaaatcac
    34981 acagtttgcg cagcggcgcg ggaagatgaa tattaataaa attccgcgcg gtgtctggat
    35041 ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga agttgtcgat attgtcatgg
    35101 cgttccgtca cccctcaaat ttaagatgac gcgacaataa ccacgattaa cacccaatgc
    35161 agcaggcagt ttttatcttt ggacagcgaa ttcagaagta tttttagcgg actgcaactt
    35221 ttacatttca gcggaagctg actcgcaaat tccccttgaa aatactccgg cagcgttagc
    35281 caccggagag gaaattattt cagcgatccc ttaaggaact gttgcagacg agggctttgc
    35341 gggttgccaa ataactgctc cggcgcgccc tcttcttcta ttttcccctg atggaggaaa
    35401 atgacgtgag tagaaacatg gcgagcaaag cccatttcgt gagtcactac caccatggtt
    35461 ttcccctctt ctgccagttg ctgcataata cgtaacactt cgcctaccag ttcaggatcg
    35521 agcgccgagg taggttcatc aaacagtaaa acttctggtt ccatcgccag cgcccgcgcg
    35581 atagaaacac gctgttgctg accgccggaa agatgcaccg gatatttccc ctgcgcacgt
    35641 tcgtctatcc cgactttcgc cagatacttc accgcccgct cgcgcgcttc ctgcttgctc
    35701 aggcccaaca cctgaatcgg cgcttccatg acgttttcca acaccgtcat atggctccag
    35761 agattgaagt gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta
    35821 tcggcgactt tgagttgacc gtctttgtcg cgcaccagat tgatcgtctg gccattgacc
    35881 acgatcgatc cttcactcgg tttttcgagg aagttaatgc agcgcagaaa ggtacttttc
    35941 cccgatcccg acgatccgat gatgcttatt acatctccgg cattcgcttg cagtgagacc
    36001 cctttcagca cttcgtgttc gccatagcgt ttgtgcaaat cgataacgtt taatttattc
    36061 tcggacatcg tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc
    36121 cgctctgcga aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat
    36181 gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta ttttcagcag
    36241 atccggcaca gtggccgtaa acgccagcgc agtagagtgc agcatcagga tcacttcgtt
    36301 gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcagc gatacatttt
    36361 aaaggtcgag aagccatagg ctctggcggc ttcaatttcc ccatgcggaa ccgaacggat
    36421 tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt
    36481 acagttcagg ccactgcgga agaaagcgtt aaggaattcg gttcccttaa caatctcaag
    36541 cgtgtacatg ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat
    36601 ataggtaaat aaccagattg gaaactggat gtatttatta ctggagacac gaccaatcgc
    36661 cagaaacagc gccaggactc cgcctatcac taccgacaaa ataagcagcc acagagtgat
    36721 cgccacacca gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt attcatgtaa
    36781 gatttcgatc acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc
    36841 agcacaccat tggaaacggt ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa
    36901 tagaacggtt cccaggtact tttgcctgcc agctgcgtgg ctttgaccac atcttccagg
    36961 ccgagtaacg aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct
    37021 ggcagggcgt aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccgcgagta
    37081 aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca ctgccataaa cgcgccacgg
    37141 aacgtttcgg taaaataagc accgtagata aaaccgagag tgataatacc agcgaccatc
    37201 ggatcgatat caatctgccc gacgcccatc gcctccgtca ccatgtttag cgcaatctgc
    37261 aaaccataga aaatcagcag catcaacacc aagtccggca cgcctcgaat cagcgtggtg
    37321 tacccttcga aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt
    37381 aaaccgatga ttacagcgag cactacagag ctgatagcca gctccagcgt gacgagcgca
    37441 ccctgtaaaa taacacctga aaacccatac aacatgctgc ctgtcctgtc gtgtgtggtg
    37501 aattaccgcc tcttgtctcc ctccggttac ccgaagggag cgggctttct ttcaccgatg
    37561 gcgattaacc accataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac
    37621 cgtcagcgcg catttcggca aaggctttgt tcagtgcctc gcgcagttcg ttatcttctt
    37681 tacgcaggcc catgccggtc cctacgccaa acagtttttc atctttaaca gacggaccac
    37741 cgaatttgta atctttaccg acgggttgtt tgaggaaacc ttcgctggca gcgacctcat
    37801 cctggaacgc ggcatcaata cgtccggcag tcaggtcaga ataaatgttg tcctgcccct
    37861 gatacgagac gatttcaatg ccttttggtg cccagtgttc attaccgaac gtctcctggg
    37921 tggtgccctg caatacgcct acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt
    37981 cagaattttt cgccaccacc agacgagaat cggcagcgta cagtttgtcg gtgaaggcaa
    38041 tttcttgctg acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg
    38101 cttttaagga cgggattaac gcatccagcg ggttttcgac aaacgtacat tgcgtattga
    38161 tgcgcttgca caactccttc gccagctcga tatcgaaacc aaccagttcg ccttgtgaat
    38221 tttttgattc aaatggcgca taggtcgggt cggtaccgat gcggatgttt tgcggaattg
    38281 cagcaagtgc cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt
    38341 ttttcataat gtcctcaact gacagtcttt ttatagggaa tatttacagg tttgttacac
    38401 ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc gccccgttag acgtattatt
    38461 tttcattaaa aatgcttaag tatgtatttc aatgcaccac gatagtgcaa caaacctggc
    38521 gtgcaccatg atgatcattc catcaggtac agcttcccag cgacgtatca gtcaccgtag
    38581 acattaaagt cgaaatactt tttcgccatc ttgtcgtagg tgccgtcctg acgcagctcg
    38641 ccaagcgcct tattgaaggc agccgtcagt tcagcatcat ctttacgtag ccctacaccg
    38701 gtgccatcac cgaagtattt tttgtctttt actgatgagc cagcaaaagc gaaatcttta
    38761 ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt catcttgtaa cgcagcatcc
    38821 agacgtcctg cagccagatc ggaatagacc aaatcctggt tggcataggc caccacatcc
    38881 acgcctttac tacgccaggt ctcgttagcg taagcttcct gggttgatcc ctgcagcaca
    38941 ccaacatgtt tacctttcag tgaatccagc gttggctgaa tcggtgaacc tttggcagca
    39001 atcaaacgag aatctgcggc gtacagcttg tcggagaagg caatctcctg ctgacgttta
    39061 tcggtaatgg aaagcgacga aataatagcg tcgatttttt tcgctttcag tgaggggatc
    39121 agcgcgtcaa agtcactggc aacccaggta catttcacct gcatccgttt gcacatctcg
    39181 ttaccgagat cgatatcaaa gccaacaaaa tcacctttag catctttcga cgagaacagc
    39241 gcataggtgg tatcggttcc gatacgtacc gtctccggta gcgccgcata gctggaagcc
    39301 gctgcggaga gaccgactaa taaagacaga gcgagaatcg acttcttcat acataaccct
    39361 caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg
    39421 caggtcttat gccatcttga cgacaacagt aacattcaac gttaaatatg ttaataagac
    39481 gttgcattat tgtcctgaag ttgaagatag caggtatagc ggttgaatcg cagcgtttcg
    39541 attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac
    39601 agggcaacag cggagttatg cgccctgcca gcgggcaaag agatcttcag gaagggttat
    39661 tgcaaactgg tcaagaacac gattaaccgt ctgatttatc acatcatcaa gggattgcgg
    39721 gcgatgataa aacgccggaa cgggaggcat aatcaccgca ccgatttctg ccgcctgagt
    39781 cattaaacgc agatggccta agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg
    39841 acgctctttc agcaccacat ctgccgcacg ggtcagtaag ccatcagtat agctatggac
    39901 aatgccggac agggttttga ttgaacaggg taaaatcacc atccccagcg tctggaaaga
    39961 accggaagag atgctggcgg aaatatcgcg cgcatcgagc gtgacatcag ctaatgcctg
    40021 cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg cctggctcat
    40081 caccagatgc gtttcgatat ctgtgacatc gcgcagaacc cgtaataagc gcacgccata
    40141 aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt ttcataaata gttgccctgt
    40201 cagacttgca ggcagacttt gcagtatttc ggcgggagtt gcaagtcagg gtgccagacc
    40261 ggcaccctca gcgaaggcat catccttcgt tatgcatttc gagattttcc acttcgttct
    40321 gacgttgcac tgctttggcg tcatcattac gtaacgtatc gaggaaatcg aggtagccct
    40381 gatcaacatc tttggtgacg tagacgccgt tgaacaccga gcattcaaac tgctggatat
    40441 ccggattttc agcgcgaacg gcgtcgatca gatcgttcag atcctggaaa atcaacccgt
    40501 cagcaccgat gatctggcga atttcatcaa cttcgcgacc gtgagcgatc agttccgtgg
    40561 cgctcggcat atcaatacca taaacgttcg ggaagcgaat ttccggtgcc gcagaagcga
    40621 ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc
    40681 cacggacgat ggagtcgtcg accagcagga cgtttttatc gcggaactcg gcgcggttgg
    40741 cattcagttt acggcgcacg gacttacgac gcagctgctg gcccggcatg ataaaggtgc
    40801 ggccaacata gcggttttta acgaagccct ggcggtacgg tttgcccaga atacgagcaa
    40861 tttccagcgc gatatcacac gaggtttctg ggatcgggat caccacgtcg atatccagat
    40921 cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc catattcaca cgcgcgctgt
    40981 aaacggaaat tttgtcgata aacgagtccg ggcgggcaaa gtatacatac tcaaacaggc
    41041 acggattgct gaccggattg tcagcacatt gacgggtaaa caactgccct tcttcagtga
    41101 tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg
    41161 ctacgctttc ggaagcgacc atatattctg tacggttctc gtcaatatca cgttttccca
    41221 gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac cataccgtgg ccgataatca
    41281 tcgccacaca ggcatacgcg ccgcggatta agcggtttgt ggcagcaatg gcagcgaaaa
    41341 tattgtcggc ttccagcggg tagtggcgga agttgtccag ctcgctggcg aagatattaa
    41401 gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg tttttcttca aacagttttt
    41461 tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg ccatacgggg
    41521 agttaacgta aaacggctgc gcttcagagg cgctggagct gccagccgta gggtaacgca
    41581 catgaccaat gcccatattg ccctgcaaac gctgcatatg gcgagcttca aatacatcgc
    41641 tcaccagccc gttcgcttta cgcaaacgga agcagttatt ggcatctatg gtgatgatgc
    41701 cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc atcataaatc gactggttaa
    41761 ccggcataac accggcgata ccgacaatac cgcacatacg tctttttcct cgttaagcca
    41821 catctcagag cacttacgct ctgggcaaga aacttgacga gctttgcaga taatcaaaaa
    41881 accatctgat gataaaactg aattgcggga tcagctgtga tttgctccag tcttcacttt
    41941 tcgacacccc ggtaaaggag tcgagaaaga agagaatggc ggcaacaatc aacacaccgc
    42001 gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc tgacaacccc gttttctcca
    42061 ccaactggcc tatcacgaag ttcacgatag caccaacgat cagggtagcg ataaacagta
    42121 ccgcgatggc aatcccattt cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca
    42181 ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc agcgataacg
    42241 cttcacgaac aaagccgtgg atcaggctaa ccagagagga aaaagcaatc accgcgatta
    42301 tggcgtaatc aatccagacc atatgtgtcc cacgatttta cgccctgtca tcctgttcgg
    42361 ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat ttccttcccg cgcatcaata
    42421 aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc ggcatcgtgc
    42481 aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta cggttctaac actgcccaag
    42541 cctgataaga cgcgccagcg tcgcatcagg caagactgta ttaattcggc gtatagccca
    42601 ttaccacgcc acttaagcca gaaagttgct tcaactcacc cagcgaacct ttcagcttat
    42661 ctttcgaggc atccggccca accagaatac gggtaatttt accctgcact ggcgtggatg
    42721 gcgacgtata aacccgataa ccggcaccgc gcagcttacc gacaatctca ttcactttat
    42781 cggcattttt cagcgcaccc agttgcacaa cataggcttt acccgttggc gctgcttttt
    42841 cttccacgac cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc tttggtttag
    42901 gcggctccac cggtttcggc ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg
    42961 tgttattggc tgcaatagtg gccggatcga gcgacggtgc tgcggcatca cctgcccgca
    43021 cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg gctggcatca
    43081 tatcaggctc atcacgatcg cccgctttcg gcaccagcgg gatagccgcg aactcatcct
    43141 gataatgttt tttctgcccg tccagcagcc ctggaagtac aatcaccccc agcgccacca
    43201 gcacgatcgt gcccactaac cgattctgaa acttacttgc caccgcttct cctcgcgtca
    43261 atcacttcca tgacatgtgc gaccgtgtgg aaagaaccac acaccagcac ggtgtcttcc
    43321 gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg
    43381 ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca
    43441 caataccagt catcaaccac gcttttcaac caggccagag ttccggcaat atctttatca
    43501 tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg
    43561 gtgagatatt ccgccgcatg tggattatgc gcgacatcaa aaataacgcg tggcgactcg
    43621 ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt
    43681 tcactgactt ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc attcggttgc
    43741 gggacaagcg gcaacggcag attttccagc gtgccgtgag catcgctaaa cgcccagtca
    43801 tgatcggtga cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc acctttttcc
    43861 tgcgccacat cagcaatggt agaaggcatt tccggctcac cgacaattgc cggtttttcg
    43921 ctgcggaaga tgcctgcttt ctcgcgacca atactttcgc gatctggacc cagccagtcg
    43981 gtatgatcca gcgcaatact ggttactacc gcgacatcgg catcgacaat attggttgcg
    44041 tccagacgac cgcccagccc tacttccaga atcaccacgt caagttgtgc ctgcttgaac
    44101 agccacaacg ccgacagcgt accgtactcg aaataggtca gggaaatatc accgcgtgcc
    44161 gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg gccctgcaca
    44221 cgtacgcgct cggtataacg caccagatga ggcgaactgt agacgcccac tttgtaccct
    44281 gccgccatca gaatcgactc cagcgtacgg caggtggtgc ctttgccatt cgtacccgca
    44341 acggtaaaca caaatggcgc tggtttcagg acgccaagac gcgccgcgac ctggctcacg
    44401 cgctcaaggc cgagatcgat agttttactg tgcaggtttt ccagataaga aagccacgaa
    44461 gccagaggcg acgcggcttg aggagtgcgt ttgataatca tggtatccgc tgattcgtta
    44521 cggtgagaat agcaaaaggg cagagccagt ggccctgccc ttatcagtta tcaggcctca
    44581 ggttcctgat ccggtaccgg gggtaccact acgccttcac gcggcgcttc aggattcggc
    44641 gctggcagat tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat ttccggacga
    44701 cggacgatca tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg gaatctaggc
    44761 ggcagttttt cgcgaacggt ctgttcgata acacgcggac cggcaaagcc gattaacgct
    44821 ttaggttcag cgatgttgag atcgcccagc atggcgaagc ttgcagaaac accgcccatc
    44881 gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct cctgcatttt tgccagtgcc
    44941 gcagaggttt tcgccatctg catcagcgac atcagtgctt cctgcatacg tgcgccacca
    45001 gaggcggaga agcagatcag cgggcagtta tcttcctgcg cctgctcaac ggcacgcacg
    45061 aaacgtgcac ccacaacaga ccccattgaa ccgcccataa aggcgaactc gaatgccgca
    45121 gcgacaaccg gcattccata cagagtgcct ttcatcacca ccagcgcatc tttttcgccg
    45181 gtttctttct gcgcagatgc cagacggtct ttatacttct tggagtcacg aaacttcagc
    45241 tcatctttcg gctcaagctc gctacccagc tccacaaggc ttccttcatc taacaggcta
    45301 tgcaggcgat tacgcgctgt catacgcatg tgatggtcac acttcggaca gacctcaaga
    45361 ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc tatcacactt agtccacacc
    45421 ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg ttcaatccag
    45481 ctcattaggg acctttctgt ctgaacctgg ttcgatgcca gttttatctt tggggacgca
    45541 taatgccatt tttgccccca acagaccatg aatgttgcac attaaaacat aacagcccga
    45601 aactttggat aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg
    45661 cagcgcgttt gtgacggatt atttcgatga cgcccggcaa aatagaaacc acaataatcc
    45721 cgacgatcag cagcttaagg ttatcctgaa ccatcgggat tgtaccgaag aaatagcctg
    45781 cgtaggtaaa aagcagtacc cacaacagtg cgccgatcac gttataagca gcgaaatgac
    45841 ggtacgacat gtggcccatt cccgcaacaa acggggcgaa cgttctgacg atcggcacaa
    45901 aacgggcgag aataatcgtt ttgccgccat gtttctcata aaactgatgg gttttgtcga
    45961 gataactgcg acggaaaatt ttcgaattgg gattactgaa taacttttca ccgaacaacc
    46021 gcccgatagt gtaattgacc gcgtcaccca caatcgcggc gatcagcatc agcactacca
    46081 tcatatggac gttgagatcg ttggtttcca gtgatgccaa cgctccggca acaaacagca
    46141 atgaatcacc cggtaaaaac ggcattacca ccagaccggt ttcacagaac aaaattaaga
    46201 acaaaatggc ataaacccag acgccgtact ccgcgactag ctctgccaga tgcacgtcaa
    46261 tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat atttgcctat gccttgtact
    46321 cgtcatcttt caggctgtaa ctacgttggc tacagcctga aatgttccga gcattattct
    46381 cgttagtccg ccagaaatag cgggcccatt ggcggttttg gaagatcata ccggtcaggg
    46441 taatccaccg cgaccagata cagcccttcc gcttttgccg ttgctgccgc cagcgttctg
    46501 tcctttgccg ccagcaactc tgctatccag ctctccggct ggttgtgggc accgacttcc
    46561 atcaggctgc cgacaatatt cctgaccata tgatgtacaa aggcattcgc tttgatatca
    46621 accaccacat aaggaccgtg acgcgtgaca ttaatatgca taacgttgcg ccacggggtt
    46681 cgggactggc actgcaccgc acggaacgag gtgaaatcat tctcgcccag caagcattgc
    46741 gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa aatgggttac ccctttactc
    46801 agtaccgccg ggcgcagccg atgattgtag atgatgtagc gataacggcg agccgtggcg
    46861 ctaaatcgtg catgaaaatc atcaggtaca gctttaaccc aacgcacggc gatgtcacca
    46921 ggtaaattcg catttacgcc caaggtccac gccgcgtctt tgcgctgcgc ggttgtttcg
    46981 aaatgcacca cttgcccggt accgtgtacc cctgcgtcag tacgcccggc gcagaagacg
    47041 gtgatgggtt cgttcgccac ctgggagagc gccttttcca gcttctcctg cacactgcgg
    47101 acttcattct gccgttgcca gccgtaatac ttactgccgt cgtactcaat gcccagcgca
    47161 attttataaa ctggcagttg ttgctggtcg gacattagta cagatactcc tgcaccagtt
    47221 tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc
    47281 agaactggac ttgctccggc ataccgtagt cattacgcac gcagccaaca gaaagatgcg
    47341 gcgtacccga agcatcccct acctgagtcg ggaattcgtt ctcttcagag agcacaatat
    47401 cttcgccctg agcaaacgca tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa
    47461 agttgaccat ctgggcatga ccgtagaata ccggtgcctg gacgacgctt gccgaaatca
    47521 tcagcccttc gtcctgcagg attttgcgta cttcgtcaac gatacgacgt tcttcacgca
    47581 cgctaccttc gctatccggc aacaacggca gcatattgaa cgccagttga cgaccgaaga
    47641 aatcttcttc gtcaatcgga atgccgttga gcaatttcgc actctgcccc gctaacgcat
    47701 cgaccgcttt tttgccctgg gcggaggctg aaatcaggct ggtaacactg atacgcgaca
    47761 aaccgccctg atcgattaac ggtttcagtg ccgccagcag ctggctggtc agactgtctg
    47821 gtacggcgat gacattccgg ttgcggtaat cggtcagtac aaacgggttt acttccggca
    47881 ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct gctgtcgatc accaggcaac
    47941 ctgagttggt cgcttcttca acccaggcag cggtagcttc tttgcctgcg acaaaaaatg
    48001 ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg cacggtgatt gttttaccac
    48061 caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt tccccaaccg
    48121 ggaactgacg ttcagccagc gtttcaagca gggcttcgcc catagcgcca gttgcgccca
    48181 ggacggcaat gttccagcct tcagacatgg tggtttactc cagaaatagc aaagctccct
    48241 gccaatgtta cagcagagag catgaagaag agattaacgt gccggatgat gaacggcgtt
    48301 aaaacccagt ttacacagca atgatgccgc actggcgtca tcacaaatta catacagaga
    48361 tgaccattca cggcgctcaa gatagttttt gcgcagttta tcgaactcac ccggtatccc
    48421 ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct
    48481 tttcagcgtc ggttgatcga gcggaccatg cagcgtaatg cggccaaact caggcgcggg
    48541 cagcaacgta tccagcgcaa cgtgttgttc atgcccaata aacttgctat aggcttcaaa
    48601 cacttgcgtc gtgccacgcg ctttaccctc cagggtatag cctgcgatat gcggcgtgcc
    48661 gatatccact tttgtcagca attccacatt aagttccggc tcgccttccc agacatccag
    48721 caccacgctt aacttctggc cttcattcag gcaggtcagc agcgcggtat tatcgacgac
    48781 tgcgccacgg caggcgttaa tcagaatcgc tccgggcttc aggctacgga tcagtttttc
    48841 atccgccaga tgtagcgttt tgtacggacc atctttaaag agtggcgtat ggaaagtcag
    48901 aatatccgcg cgctggacta actcatccag cgagcggaaa tcaccctcat ccccacggtc
    48961 ggcgcgaggc ggatcgcaaa gtaaggtttt aatccctaac gcttccagtc gcgcctgcaa
    49021 tcgacgtcca acgttaccaa cgcccacgat ccccaccgta cggtcgtgca gtgaaaatcc
    49081 atcgcgttcg gcaagcatca gcagggagga gaaaacatat tccaccaccg caatcgcatt
    49141 acagccaggt gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt catcgacatg
    49201 gtcggtcccc gcagtggcag ttccaacaaa tttaatgggt tttcctgcca gcaaagattc
    49261 attcactttc gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt gagcgacggg
    49321 gattggacgc ccgggaaccg cgatcacctc acccaaacgg ctaaataagt cgcgggcata
    49381 aggcatattt tcatcaacaa ggattttcac gtttgtgtta cctgtatgag acgagagtta
    49441 accggacaag tatgccataa tctcgcggcc aggcatactt gcgaagattt caggtataag
    49501 gatacgtaat gatacaacct atttccggcc ctcctcctgg gcagccacca ggtcagggag
    49561 ataacctgcc gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt acttcgctgg
    49621 aaagcttaat gacgaaagtg acctcactga cgcaacaaca aagagcagaa ctgtgggcgg
    49681 gtatcaggca cgatattggt ctgtcgggag attcaccgct gctttcgcgt cacttccctg
    49741 ccgctgagca taatctggcg caacgtctgc tggccgcgca aaaaagccat tctgcccgcc
    49801 agcttttagc gcaattaggg gagtatttac gtctggggaa tcatcgtcag gcggtcacgg
    49861 attatatccg tcataacttt ggtcagacgc cgctgaatca gctctcaccg gagcaattaa
    49921 aaaccattct caccctgttg caggaaggga agatggttat tccgcaacca cagcagcgcg
    49981 aggcgaccga ccgtccttta ttaccggcgg agcacaatgc gctaaaacag ctggtgacca
    50041 aacttgcggc ggcaacgggg gaacccagca aacagatctg gcaatcgatg ctggaacttt
    50101 ccggggtgaa agatggcgag ttaattccag cgaaactgtt taaccatctg gtgacctggc
    50161 tacaggcgcg tcagacgcta agccagcaaa atacgccgac gctggaatca ctacagatgg
    50221 cgctaaaaca acctttagat gccagtgaac tggcggcgtt atcggcatat atccagcaaa
    50281 aatatggcct ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat attcttaatc
    50341 agctttatca acggcgggtt aaagggattg atccgcgtga tatgcaaccg ctgcttaatc
    50401 cttttccacc gatgatggac acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac
    50461 tgttagtcgc gattatcctg atgctggtct ggctggttcg ttaaccccga cgaaatgaca
    50521 gtatcgtgac aataataccc agcaccgcag agatcgcccc ggcaagaaat accgaagagt
    50581 aaccaaacgt ggtcgccagc attcccgcca gcggcccgga gacgccgagg gcgatatcct
    50641 gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca
    50701 cctccacgcc cagcgcagga aagataagcg aacatccggc tccggttaac gccgcgcccg
    50761 ctaatgcgac ccatgcacct ggggcttgcc agagcagcaa caagcccacc gtttctacaa
    50821 gcagagagac aatcgccact ttcacgccgc caaaacggtc cggcatccag ccaaacatga
    50881 cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc
    50941 ctttgctggc aaagtagagc gaaacgaaag tcccgataac cgcaaaacca acgccttgta
    51001 gtgccagccc taaccctggt ttccagataa gcccgacaac gctccacagc gatggacgtt
    51061 ctcccgccag ggccggtact ttgcgcactg tgccgttaca ggcccacgcc agtaagggta
    51121 ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa
    51181 gcggcgcacc aacagcgagg gcaccgtaaa tcgccattcc gttccatgac atcactttgc
    51241 cagagtgttt tggccctacg atgcctaacc cccaggtcag agcgcctgtc agtaactggc
    51301 tttcaccaaa cccaagaatc aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg
    51361 agacaggcaa aatcgccgcc agcagcaacg cgccgccagc cagaccacaa gctaacatcc
    51421 cctgaagcgc cgaacgtttt gcgccatatt gatcggccag tcgcccggcg taaccacgcg
    51481 tcagcaccgt agccagaaac tgaatcccga cggcaatgcc gaccatggta ttgccatagc
    51541 ccagatcatg atgaacaaac agcgggataa ccggcaacgg caaccctacg gtcatgtagg
    51601 tgagaaaaac cgcaaaagag atgcggaaga gcgaaaaact ggcagaagat cgtgtttcgg
    51661 tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg agtatccccg
    51721 tctcatctct ctggtttcag ggttacggtg cgttggcagg atttaacgcg tacgtctttt
    51781 cagaaggaaa tcgacaaagc gggaagtttg cctagaactg gtggcgattg tcaatgatgt
    51841 gaaaaaggga accatcaggt tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa
    51901 cgtcggatgc gacgctggcg cgtcttatcc gacctactcc ggcctactgc gaattaatct
    51961 tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat
    52021 aacagtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc
    52081 gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga
    52141 gtagatagct tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg ctttggttgc
    52201 agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagct ctttcacgtc
    52261 gccaaccgga gtcgaagtac cgtgagagtt caggtaatcg attggggtat caacgccatg
    52321 catcgccatc tgcatacaac gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc
    52381 atcagaggtt gcgccgtagc caacgatttc agcatagatg taagcaccac gcgccagcgc
    52441 gtgttccagc tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg
    52501 gtgagcgtcg taagtacggg aggctttttc cggggtgtcg ttgtatttag tagatagcgc
    52561 acccatcgcg tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa
    52621 cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga
    52681 agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag
    52741 acaggcagaa acgccggatg ccatcgcttt agtgaccaca tacgggccaa ccgctttcag
    52801 gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc
    52861 ggaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggcccgc
    52921 atcagcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg
    52981 cacaactttg cggtcaatga ggccagtggt atccagtttt atgttgcccc agacgtggct
    53041 acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag
    53101 agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat
    53161 cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca
    53221 atagcgtaca cttgtacgcc gaacaagtcc gatcagccat ttaatagaga tatttgcgca
    53281 gccttacaca catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac
    53341 actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag
    53401 attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc
    53461 tgggaggtaa ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtgatag
    53521 cagagagcgg cttcggcacc ggattaaact tcctgacgct atggaaggca tttgatcagt
    53581 ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat
    53641 ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt
    53701 gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc
    53761 tcgatgaagg ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc
    53821 aactggacga ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag
    53881 ctaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc
    53941 caggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag
    54001 ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg
    54061 tgatggaaca gacattacca ctcccctgct ccacgccgtg gtttaatcgt acgggcagca
    54121 gcaaacggga agtagcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgt
    54181 tattacggcg cggctggcag gtagcgcttt attgcgcgga tgaagccccc gcgctgggtg
    54241 cttccggcaa tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa
    54301 accgcttttt ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta
    54361 aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata
    54421 aaatcgcaca gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa
    54481 atgcggttga acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag
    54541 gtggttggct gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg
    54601 gtttgcagat ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt
    54661 tgttgaattt tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc
    54721 atcaaatcag ccgtttcagc caaacgtcga ctctcccggt gtattcggtt gccgggcagg
    54781 tcagccatat tccgacaacg ccggaactgg cagagctgaa gcaggtgctg tgctatgacg
    54841 gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc
    54901 gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga
    54961 ttgattgttt cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc
    55021 gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt
    55081 atgaggcaac actcgtggaa tatgcgtcgt tggcggagca aaaagatgag gcggtaagcg
    55141 cgccggtttt tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg
    55201 ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg
    55261 ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta
    55321 aagcggttaa ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct
    55381 tatccaacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat
    55441 ggtgctccag acgccgcaac ttacagctgt gacgcctgct gaaacaggtt ttcccacata
    55501 tcggtcacca gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga
    55561 ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct
    55621 gcgatggcta atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg
    55681 gcatggtcaa ccatgccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc
    55741 ttctttcctc attgtaaaac gtggcgctga cttcagttga gcgcttccgg ccacgggaac
    55801 tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc
    55861 gggcgttccg gaatgccatt tgtcagataa tccatcacct gcgcatgtac ccgccgttga
    55921 aacaccacac gatccggttc gaaatcccct tccagattgt cgcaactgac gttaaacgga
    55981 tatccagccg ccacgcagaa taaccagtcg aacgcctgcg gcttcacttc aacatcttca
    56041 aactggcttt gcgtctgggc atcgcgtccg tccgggcaat accagtagcc gaaatcaacc
    56101 agctcacggc gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca
    56161 taaaagccat gagcaaagac gattcggtta tacggtactt ccgcatcagc aggaagatag
    56221 atcggttcgt cgtcgccttt aatcagacgg gtattaaatt catcggcaaa gcagctatta
    56281 aaaatttcaa tcaactgctc gtagtggtgt gtactgttca ttagttcatc cccaaccagt
    56341 ggaggatctc ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gccgagacga
    56401 taacaatcat cgggcggatc agcttttgtc ctttgctcaa caccaaccgt gaacccatgc
    56461 gcgcgccgag gaactgcccc accagcatga caaagcccgt cgcccaaatc actttgccgc
    56521 cgagaataaa cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat ttggcgtgag
    56581 ccgtggcttt ggcgaggttg aatccgcaca gcataacgaa ggccagcgcg taaaacgatc
    56641 cggcagccgg gccaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga
    56701 agggtaaacc gtacatccgg cgctggcgat cttcttcacc cagctttggc atcagcaaga
    56761 aatagaggcc gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa
    56821 cgtactgtac caacagtgcg ccgctcattg agccgacaaa ggtcatggca atattgagtt
    56881 tctgatcgct taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc
    56941 cgccgcaggc ttgcagttta ttggttgcca gcgcgttagc gggagacatt cccgcagcca
    57001 tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca
    57061 gcatggcgac aaaaaagaga actcccagca acagcggaga aaccataaac aggctattaa
    57121 acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg
    57181 cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag
    57241 ttctgccccg caaccatcgc ctgatggcgg taaaggttga tcttcacact ccagactatc
    57301 ggcagggcaa cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt
    57361 gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt
    57421 aacaaaaatg cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc
    57481 tggcttccac agcgtggaga caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg
    57541 cgggcgcaag agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac
    57601 atccagtccg gtctggtggc tggcatgacc gccgttgaaa cgcccaccag cgggcatccc
    57661 catatcgcca atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg
    57721 gataaacatc accagatccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg
    57781 ataatgttcg gactgtatcg gcaacgtgtc agcgccgaca atacagccat tagaaaaact
    57841 gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc
    57901 caggctagcg ctactggcaa gcagagccag cagcgcaatc gcggttttgt tcatttttta
    57961 ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc
    58021 cattaaaacg atcgccagca tcgcttcagc gatcggcact gcacggatcc cgacacaggg
    58081 atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc
    58141 cggcacggta atgctggagg tcggtttcag cgccatatga gcaatgattt gctgcccgct
    58201 gctgataccg ccgaaaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc
    58261 gtcgcggttc tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc
    58321 tttcaccgcg ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac
    58381 cggctcgcca agtccggcgg ggacaccact ggcaacaacg gtgactttcg cgccgatgga
    58441 gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc
    58501 cgggcagaag aacggatttt gttcaaccag cgaccagtct ttgatttcca gcggaatgtc
    58561 gcccatctgg gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt
    58621 ggcaatcgcc cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc
    58681 gcgataatcg cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc ctggacggaa
    58741 gacgtcttta attgcgctgt aatcctgaga acgctggtcg gtattttcga tcaacaagcc
    58801 aatgctggtg ccagtagtaa cgccttcaaa aacaccggag agtattttga cctgatccgg
    58861 ctcgcggcgc tgggtggtat agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg
    58921 caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc
    58981 cagcccgtgc gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc
    59041 catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat
    59101 gttctcgtgc ggcaataagc tgctctttag tgagcataaa cacaccatcg ccgccgttat
    59161 caaactccag ccaggtgaac ggaacatcgg gatattgttc cataagatgt accatgctgt
    59221 tgcccacttc acaaatcaac acgccatcat cagcaaggta atctgccgcg ttaccgagaa
    59281 tgcgacgcgt cagtttcagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac
    59341 ggtattcgtt tggcaggtcg gacatatctt ccgcgtcgac atacggcggg ttagtgacaa
    59401 tcagatcgta ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt
    59461 tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga
    59521 tatctaccgc gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc
    59581 cgctaccggt acacatatct aaaatatgct gcggttgctt gctgatgagt ccggcaaatt
    59641 tattgttgat cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa
    59701 attcatgacc gcagaaccag gctttgttgg tcaggtaagc caccggaatg cgctcgttga
    59761 cgcggcggat cacgcgttca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc
    59821 gcatatcttc cgggatatcc agcggcaggt agagcgatgg taacaccagc tgtacggctt
    59881 catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctta
    59941 ccgaccagcg caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa
    60001 ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg
    60061 aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat
    60121 cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa
    60181 aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac
    60241 tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt
    60301 gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct ccgatgagtt
    60361 tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt
    60421 tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg
    60481 tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct gtcgccgcga
    60541 acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac
    60601 accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac cgaaagagta
    60661 cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gaatggttgc cgccggagtt
    60721 gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg cctacaaata
    60781 tgtgataatt caataaattg caattcactt cgtaggcctg ataagacgca tcagagcaat
    60841 actacgaact tcagttcatt caatacatac tgaaaacgac acgtcgaatc aatcggttgc
    60901 ccactactgg tattcacatt caccacaata tcgtgtagta ctttatccgt taccggatga
    60961 cgagttggcg taaccgttac cgttgattta tcacaaccgt aagaggtaaa ggttaacaat
    61021 tctgataccg cgcggcagta tgctcctctg ccatcattat cagaaaaatt actgccatca
    61081 cagagtctgc cgccttccat caggtaggtc gaaagcgtgg tggtcactgt tgaccccgtg
    61141 gactgaattc gtaacgcctt attatggctg acataatttt cactccagga gatatcgtat
    61201 gaagcgaccg gtatcgttgg gcatattgtg ttatgcactg taggggccgt atagcttcca
    61261 aatccgggta aatctgtata ggaataaaag gatgcgatcc aagggcaacc ataatcatca
    61321 gatgcaacgg taattttaaa cgtcatatat gcacaacgaa aaattttcgt atctatgcca
    61381 gggcaatttg agttattaat tgtatcaaag tcagtcagta ccaaagttgg agctgaaccc
    61441 gacccagacc aggaaataac ccgcaacgta tcgcctttat acgcaaaaga atcatatttc
    61501 ccatatgcaa cctcctttgg cgttatcacc gtaacattga aaacactgct cgctacagca
    61561 ccggttacct tgtccatggc ataataaact ctcacactgt caaaactcgc actcataagt
    61621 ttatctccag cataactctt agatgcgccg acaacactca gaacgagcag cattaggctc
    61681 gcgaaaatta tggtccattt tttcatttca gaaatactca aaggttgccg tcactgtggc
    61741 actaaagtct ccggacgtta catctcgccc gctctttgcc tgtaaccagg tattaaaatt
    61801 taggctgtta tttccctgat tcagcgggag cgtgacgcct gttgtattgt tgattggcac
    61861 cgatgtgcct gccgcagttt caataccaat cccagcgccg gatgctgatg acgaggagtc
    61921 aaaggccaga aagccgggta gagcactatc ttctgttcct gtcaacgtta ctttgacatt
    61981 aaacaatgta gaactatgac aatctttcaa ttgaaacacc aatggcactc gctctgactg
    62041 cccaaggttc attaaatcac tggcggcaat ggcaggaaaa tgtacttctg ccaactggct
    62101 accattaatc actggcgtac acgatttact gattaaattg ccggtaaagt gcaaattatc
    62161 tcctgctatt gtggttccac tgcccagcaa catcaggcta ctaataaaaa acaacgtacg
    62221 ctttttcttc attggtaatc tacctgtaac gtggcccagg cctcaaagtc cgcctctggc
    62281 agaatgacac cactcttttt tactggcaca gccgttaaca ccggtaaatc ggtttcattc
    62341 accacaagtg gcgtattgat cgtaaaactc tgccctgcct gtttgagctg aatgcctaaa
    62401 ccagtgatat tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc actccagctc
    62461 aaggtcattt ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga atacggcacg
    62521 gtttgctcat aattgctgcc attaatgtca tcaataagca catcctgaaa agttatttca
    62581 atggtttgag cgttgctaat cgtgcagtta ggcggctgca ctaaataacc gtgaaaagag
    62641 acgtttgaga gtgccatcga gcaaaagccc cacagcaaaa taagcgagat ccgtttcatt
    62701 ggtaatccac cacaattgtg gcactggcgt taaattcagc tgccgctaac gttgtaccac
    62761 tctgtttcac cggcacagct tccagaacgg gaactcccga ggaaaaatta aatggcaacc
    62821 atgagccaga ggttaagtcg agtattgtgt gatcactact tttctgaacc cgaataccaa
    62881 acccaggaat acccgtgctg ataactgttt caccgttaat cacgaccgtt gtcgcctgca
    62941 tctgtagacg caggtcatcc attgccagat tgttacaaac aagcgagtag tcaatgggac
    63001 gtttataatc aaccctattg atcttattaa tgaatacatt accaaactca actgccgcac
    63061 catttaccgt acaaggagga ggggcatcga caagcacccg taaattcata cttactgact
    63121 tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca gttaccgccc
    63181 agacagaaca agatcctttc atactggctc tcccggcctc agcctttgcg gctctgctct
    63241 tcatcgacct agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg agcgccatag
    63301 tcgttgacat aggtcagtac cggcacgcta cccatcttca cattcagcgg tgcagaggtt
    63361 ttcggcgaca tcactattag cgagaaaccg gctgccggat tgccattttt ctgtgtgctg
    63421 gcattactga aaatgacgta atacgacgtc gggttattga cggtaaattc ctgaccgttg
    63481 cgggttaaag taatcttata ttgctagggg tttttcatgc tgacgttctc cagcgccttc
    63541 ggtcgccaga acaacttaat acgcgtttgt agtgctattt gcagcgtgtt ggctttgttt
    63601 gattttggcg ggatctcgcg cacgttgaag tagaacaggc tttcacgatc ggcaggcaat
    63661 ttgttgatat ccggcattcc ctgcactttt acctgcccgt tcatcattga atcgatgcgt
    63721 tgcaccggtg gcagaacagt taacggtgaa ctgatcttgt tacccttttc atcttcgatc
    63781 cagctttggg ccagataagg cagtttcgga tcgttattgc gcaacgtcac gctgattgat
    63841 ttgtcgcttt cattaaatat caaacgggta cgatctggcg ttacagaagc ctgggcagac
    63901 aaactggcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc agagcaaagt
    63961 aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag aaacaggggg
    64021 attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt catcaggaac
    64081 aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa acagatcggc
    64141 aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga acacactcat
    64201 gtgttcgcca ggtttcaccc cagccagata aacattgccg tcatcatcga caaggcccac
    64261 tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa
    64321 acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt agccaatcgc
    64381 cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt ccggcagttt
    64441 gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa cgacggcttt
    64501 accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat cggcaacgcc
    64561 atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc
    64621 gtgggcagta agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt attgcccttc
    64681 atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt aatagccgtc
    64741 aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg cgtcatcgac
    64801 acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagtacc gttatagcta
    64861 acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt gtcggcctgg
    64921 ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat actgcccata
    64981 ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc ccagtaggta
    65041 tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg gttataagtg
    65101 gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact ggcatccaga
    65161 tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa agtaacgcgg
    65221 ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc gtccagcgaa
    65281 cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg
    65341 accgcgccga atgtagacaa atcgcgaccg acaccaagcg ccgcagactg atagttttca
    65401 tctcccagtg cgccgccata taacgaccag ccgttagcga tcccccagga agcttcagca
    65461 ccagaaaaaa attcaccctc gacatggtgt ccccactctt gcggacggcc catcatgatc
    65521 ttatagcgaa cctgacctgg acgagtgagg tatggcatcg aggcggtgct gatgtcatat
    65581 tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga gacagaatca
    65641 ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc
    65701 atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag
    65761 ccgcgcagat tgggaggcaa catttgatcg tcagtactga cactgccacc aacatagtta
    65821 aaaccatcaa aaatatcgga tctgaggtaa tcctcgccca gcgccagttt ggcttttaat
    65881 gatggtaacg cccgcctggc atagtagcga ctccactccc attttttttg agtttcatcg
    65941 ccgctgaatt catcgtcatc attactgcga gtatgttgat agttagtctg ccagtcagca
    66001 cgcatacgcc acggccctag gttaacccca accgtcccgt tgccgctgat ctcgttacta
    66061 tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc
    66121 ccggagatgc cgtcatccca acgtgaaggc ggatcccagt cgggataagt atattcgagg
    66181 taagcctgtg gcagtgaaat gactaatgcg gactggctta aatcagcctt aatttccatg
    66241 ccttccagtt gaccggattt caggcattta gcatcgtggc tccattgcag atttttcgcc
    66301 acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg
    66361 ctcgcgtcat cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta
    66421 tttagttgaa cctgtaaatt gtattttccg ggctcaacat atccctagct ggaaaaacgc
    66481 ttcagatcaa tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg
    66541 tcttcagccc agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga
    66601 atccgtaatc gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt
    66661 tcggtcaaaa tacatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg
    66721 taagtaatct ggaaggtggt atttgcttca aaattaccca gatctggcgc atcagctgcg
    66781 ccgaccagcc aggcgttaaa gttcagtgtc tgcttcgctt tgcctttgtt ggtagaagtg
    66841 tctttcacta ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca
    66901 ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa
    66961 taattgccgc tatttgcaga agaaacggta ccagtaaagg tagtggtcat tgtttcctga
    67021 gtatcaaata cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta
    67081 tttttcagtt tttcggcacc gatatcaccc atatcaactt ccagtttgtg atcgtctgga
    67141 acgatagaac acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc
    67201 gcagcgattg ttgccgtaga ggtgaacacg cccatcacca ttgccgcagc aatagctgtt
    67261 ttaacaaact tactcattat ttgtccttaa actataaata attaaaaata taaacattgc
    67321 aatacattga ttcagtcaat agccaatgtt tatacccagc cattgaatac ggcatgcgac
    67381 ccaaaaagaa caggtcgtag aaaattgtat tcattggtgt ggggccgcat agtgatgttt
    67441 ccacacccct gatccaattt aattttacta aagagtaaat gtagatttaa ttaatatatt
    67501 gacagaagaa aggttatctt aaggacatcc ttaaaaaata cggaataagt atttagaaaa
    67561 aatagaaagt atatcaataa tgaatatcaa cagcttaaaa aacaaacaat gaacatataa
    67621 tgcgattggc atttatccac ttttgacaat ataatatata aacactatag ccagaattta
    67681 atcaagtttc aattacatcc tgcctgatta ttcaactcta ttttctctat tatctgtatt
    67741 aataaatgac aatgagtgtc aaacagatat tattcatttc aacatgtaac tttgggaaaa
    67801 atcaagatac gcgcagaaga ataatttccg aatgaatgta gaaagtgttt tatttataga
    67861 taaggagagt cttcgatagt agtgaggttg acagatatcc gctgcctaat gagcaattat
    67921 gcccaatgaa tctacctcat tgggcataat ttggaacaca tcagatagct tttgccatct
    67981 tcagattaca cgggctcatc tgccagttga atgtaccgtt tccgctttca tcaagcgtta
    68041 cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat aactcggcaa
    68101 ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca ccttcattgg
    68161 tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt aactccggca
    68221 gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt gtttgctcgg
    68281 ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct ttcagccagt
    68341 tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacc gaatcactgg
    68401 cggaatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg caccgctttt
    68461 gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc
    68521 attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg cgtaagtata
    68581 gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt catatagctt
    68641 tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc acgcgcgccc
    68701 atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc cagtcgttgc
    68761 attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg tccaccgaga
    68821 aatggcggaa aaccaatgcc aaataccgca ccaatatccc cgtcacgcac gctacggata
    68881 acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca ccgttcagca
    68941 acctgcggtg cggagagtca cccctgccct tgtgcgccaa tgagcgggta aatggcagga
    69001 tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc ccgaccattt
    69061 tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa
    69121 cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc gattcctacc
    69181 tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcactagcgc ggcatcaatg
    69241 tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta aggcgctaag
    69301 atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt
    69361 ttcgccagtt ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg aggaataatc
    69421 tctaccagcg gcattttttc caccggactg aagaaatgca ggccaataac ttgctcaggt
    69481 cgcgcggcgt gagcggcgat atcaccaatc ggtaaagatg acgtgttcga agcaaaaatg
    69541 gtatgagcgg cgcaattttg ctccacttcc gccaccatct gttgttttaa ttcgagattt
    69601 tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg
    69661 gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg gcgaactttg
    69721 ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg gttgatatct
    69781 ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc gccgcccatc
    69841 aagccaccac ctaaaattcc cacgctgttt aatgccgcag gcggtgcatc actaccggga
    69901 tctttcttca cgtccgtact ggcaaaaaag atactacgca aagcttgcga ttgcggcgtc
    69961 atcgccagtt cgccaaaggc gcgagcttca gcgtcatagc cgctgctggt gccctgcgct
    70021 aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc ttgagttttg
    70081 tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg ccccgccaga
    70141 atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc cagctcaaca
    70201 gcggcttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag ctttaatgcc
    70261 tgtttcgccc gaagttgttt tccggtgaga atcatctcta atgctgtact gacgcctatc
    70321 agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg tacttcagac
    70381 aaaccgagca ccgttttagg atcgtcagta caaacgcgac cgtggcacgc cagcgccagc
    70441 tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaat gggcaaagca
    70501 tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt
    70561 ttgcagttgc caatcatgtt gatgtctgct ccggcgataa aattgtccgg tttcgcggag
    70621 ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat ggcgcgcacc
    70681 tgcgaggcaa attccgcctt cagggtattc attttctcat ccggtacgtc gatggtgatg
    70741 atagcaatgt tgtccagacg aacattaaga gtaaacgctg atgccatttc cattattccg
    70801 cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact aaaccaaatc
    70861 caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg
    70921 cgaagggatg tccgtaagca atcgaaccgc caagcacgtt aaatttgcta tcgtccactt
    70981 cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta cccagcaact
    71041 gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat gtcagttcgc
    71101 tcatcgtcaa accggcacgt tccagcgcca gcggtgttga ccaggctgga ccgagcaaca
    71161 tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc agcggcacca
    71221 gccctaattc tttcgcccgg gattcagtca tcaggatcac cgctgccgcg ccatcggtca
    71281 gcggcgtact gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct
    71341 ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca agcggttgtt
    71401 tataaggagg gataaaggca gtcatcacct cttctttgag ttttccgtct gaccatgcct
    71461 gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt
    71521 aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta
    71581 ccgcaggcgg tacgggcatt aagtcgcgca aacgcaggcg agagaagagt ttcaggcgct
    71641 ggctcatggt tcgagctttg ttgacatcaa ccagcacgtg cgccagtttt ttactgacgc
    71701 caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg atagttcccg
    71761 ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga
    71821 cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca cgcgcaatgt
    71881 tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc acttcagcgg
    71941 ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca accgtgggaa
    72001 tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta acaatggcga
    72061 tacgatcgcc ctggcgggta accagtggta aaacctgacc cataacactc ccctgtaaaa
    72121 aaataaataa agtggtctga cctgatcata gtcttaacca tttttttaca tttagccaag
    72181 cggagaaaaa ggaaagtggg agctatgaca cagtgaagac agaagaaaga aaagaaaacg
    72241 cccctgccag caactgacag gggcgtacgt acccgagagg aattaacgca gacccagctg
    72301 gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc
    72361 agactcttca gtgaaagtcg gggagatttt gcacggttcg gattccacgc tacgggattt
    72421 ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca cgcggctgta
    72481 agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg
    72541 ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc
    72601 cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg
    72661 caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga
    72721 atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta gatcatattt
    72781 gaaaaaagat ggaaacatac ttgcaacatt ctagctggtc cgacctatac tctcgccact
    72841 ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa
    72901 catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta
    72961 caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct
    73021 ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa
    73081 ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg tttgaccgcc
    73141 cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc ggaacgtctc
    73201 cgtctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc
    73261 actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc tctaactatg
    73321 gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt acaactgacc
    73381 ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca tggagctttg
    73441 gtcttggttt caacgccatc tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg
    73501 ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct caagggcaag
    73561 cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat ctgaacggta
    73621 accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa aataaccgct
    73681 atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc
    73741 ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt ggcgcaacgc
    73801 aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt tataaccgtg
    73861 ttgatccaca gtgggcgatt cactatagcc tggcttacac cagctggagt cagttccagc
    73921 agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa ggctttaaag
    73981 atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg accttccgta
    74041 ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc tccattccgg
    74101 accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa gatgcttcag
    74161 tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa ggcccatacc
    74221 agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat
    74281 aacgcgttcg cctagataaa gtcacctgct atgcaggtga ctttaactcc cccacttcac
    74341 cgaaagtagt ttcctcattg cttgcctcct gagttttgca aacagcctga tggcagcttg
    74401 ccctttcaaa tcagtaagta gtatccacag acaaacctga agggaaatgc atattttcaa
    74461 gcgttctgct cgtccttctc aaagagttta cttttctgca tttccaggat actcaacccc
    74521 cctggctatt gtgcgcttat acactcaaat taaagatagg ttctaaataa atgagcgttt
    74581 tttgatagtc tatttcatta ggtaatatat atttgtaaca aatcaatcaa catggaataa
    74641 aatcatgcta ccatctattt caatcaacaa taccagcgca gcttacccag aatccatcaa
    74701 tgaaaataac aatgatgaaa ttaatggatt agtacaagag ttcaaaaacc atttttaatg
    74761 gtaaggaagg aataagcacc tgtattaaac atctacttga gcttataaaa aacgccatac
    74821 gagtaaacga cgatccttat agatctaata ttaataattc ctcagttact tatattgata
    74881 ttggctccaa tgatacagac catattacta ttggtatcga caaccaagaa ccaatagaat
    74941 tacctgcgaa ctataaagac aaagaactcg tccgtactat cattaatgac aacatagttg
    75001 agaagactca tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa gaaatatatg
    75061 atggagatcc tggttttatc ttcgataaga tatcacacaa actcagacat acggtaacgg
    75121 aatttgatga gagcgggaaa agcgaaccaa cggacttatt tacctggtac ggtaaagata
    75181 aaaaaggcga ctctctcgct attgtaatta aaaataaaaa cggaaatgat tacttatttc
    75241 tcggttacta cgatcaggac gactaccaca ttcaaagagg aattcgtatt aatggtgata
    75301 gtctcaccca atattgtagt gaaaacgcca ggagtgcttc agcgtggttt gaaagcagta
    75361 aagctatcat ggcagaatca tttgcaactg gttccgatca tcaggttgta aacgagctca
    75421 acggggaaag actgagagaa cctaacgaag tttttaaacg tttaggtcga gcaataagat
    75481 ataattttca agtggacgat gcaaaattta gacgcgataa tgtaaaagaa ataatttcta
    75541 ctttattcgc taacaaagtt gacgttgacc atcctgaaaa taaatacaaa gactttaaga
    75601 atctcgaaga taaagttgaa aaaagacttc aaaatcgcca gacgaaatat caaaatgaaa
    75661 ttaatcaact atctgcacta ggtgttaatt ttgatgatat ttaaaattat ttttccagtt
    75721 ttttgatttt tcaacgcctg aatatcggaa atagtcagtg acaaacatca gtatttttac
    75781 atcattacgt ccgggtcatt gtcggtttat ctccttttac ttgtggtaat tgagggataa
    75841 caacaaaaaa ggtgagtttt gcgactcacc ttttttattt gtttcttatt cagaatcgat
    75901 atcttttaaa tcatcctgaa tcgcttgcgc gttcggattt tcctgcggtt tgagttcgcc
    75961 gccattagcg atgaaatcat gacgctggaa gtacgcttcg cgcaccataa tataaggatc
    76021 ggacgactga cgcagcagac catcggaatc cagcagctga gcgcgggttt cgatcccttc
    76081 aagcgtccat ttacccacag acatcggcca ggtcagccag gaaagaaccg ggtaaagagc
    76141 atccgccata tcaccaccgt catcacgcag cgtgaagcta ccgtagaacg gtaactgaac
    76201 gtaaggccca taacccacgc cataatgacc aagcgtacta ccgaagcggt gaggttcagt
    76261 ccgttgcagt ttcgggttcg ccatccctgc aacatcaata aagccgccca tccccaaaat
    76321 ggtgttcagg aaaaagcggg taaagtggac catcccctga taagggtcgc cctgcaagaa
    76381 gtagttaacc atcaccgcag gttcttcaag gttgccagta aagttgctca aaccattacg
    76441 cgccggttgc ggaacataat cacgccaggc gacagcgacc ggtcgaacaa tatacgggtc
    76501 taatacgttg aagttgaagt tgtacatggt gcggttgaac ccttctaacg ggtcagaacg
    76561 cccttgctga tctgtaccgg aactcgcaca ccccaccaga agcgtagttc ccagagcaag
    76621 cgccgacagg cgaagcttca taaatgtctc cctgtttttt tatggcttat gcagtttgcc
    76681 atccatgacg gaacgatacc gtatccgcct gtttaggtgt gggcgattgt aacagcacgt
    76741 caactgatgt ccagacgccc tgatttgctg atttgatcat agcctggtaa tcgccgccct
    76801 gtaggctact tgattctata gaaacagaaa aaggcaaacg cagccttttc tacattttca
    76861 gagtaactcc cgccagttgc gagcaaaaaa gccgctacgc tttagctata cgtgctaatt
    76921 caagagaaga gaccatggac aacgacaaaa ttgatcaaca cagcgacgaa attgaagttg
    76981 agagcgaaga aaaagagcgc ggcaaaaaaa tagaaataga tgaagaccga ctcccctccc
    77041 gagcgatggc aattcatgag catatccgcc aggatggtga aaaagagcta gaacgcgacg
    77101 caatggcgct actgtggtca gccattgcgg cgggtctgtc gatgggcgct tcgttactgg
    77161 caaaagggat atttcatgcc gaactggaag gtgtgccggg cagcttcttg ctggagaatc
    77221 tcggttatac ctttggtttt attatcgtca taatggcccg ccagcaatta tttaccgaaa
    77281 acaccgtgac tgcggtacta cccgtcatgc aaaaaccgac aatgagcaac gttggcttac
    77341 ttatgcggtt atggggagtc gtgctgctgg gtaatattct cgggactggt attgctgcgt
    77401 gggcatttga atatatgcct atcttcaatg aagaaactcg cgatgcattt gtcaaaatcg
    77461 gcatggatgt gatgaagaac acccccagcg agatgtttgc caacgcgatc atttccggct
    77521 ggctgatcgc cactatggta tggatgtttc ctgcagcagg tgcggcaaag attgtggtga
    77581 ttatattgat gacctggctt attgccctgg gtgacaccac ccatatcgtg gtcggttctg
    77641 ttgaaatcct ctatctggtg tttaacggta cgctgcactg gagcgatttc atctggccct
    77701 tcgcactacc tactttagcg gggaacatct gcggcggcac ctttatcttc gcgttaatga
    77761 gtcatgcaca gattcgtaac gacatgagca acaagcgtaa agcagaagca cgccaaaaag
    77821 cagaacgtgc ggaaaacatt aagaaaaatg ataaaaaccc agcataaatg gcgagggttt
    77881 aagcaatcga gcggcagcgt acttaccccg cagtccatta gcgggtatac tcgccgcatt
    77941 gtcctcttag ttaaatggat ataacgagcc cctcctaagg gctaattgca ggttcgattc
    78001 ctgcagggga caccatttat cagttcgctc ccatccgtac cagtccgcaa aatcccctga
    78061 atatcaagcc ttccgtatat tcacagttcg tcatggttcg cgtcagatcg ttgacagccg
    78121 cactccatga cgggtaaaaa gtggataaaa taattttacc caccggattt ttacccatgc
    78181 tcaccgttaa gcagattgaa gcagcaaagc cgaaagaaaa accataccgc cttctcgatg
    78241 gtaatggcct gtacctttat gtccctgtat ccgggaaaaa ggtatggcag cttcgctaca
    78301 agattgacgg taaggagaaa atcctgaccg tcggaaaata tccgcttatg actttgcagg
    78361 aggcaaggga taaagcatgg actgcgagga aagacatctc ggctggcatc gatccggtaa
    78421 aggcgaaaaa ggcttcgtct aacaacaatt catttagtgc gatttacaag gaatggtacg
    78481 aacacaagaa gcaagtctgg tcagtagggt atgcgactga acttgcaaaa atgtttgatg
    78541 acgacatttt acccatcatc ggcggccttg agattcagga tattgagccg atgcaactgc
    78601 tggaagtaat ccgcagattt gaagatcgcg gtgc
//
LOCUS       DAEEDS010000004        75611 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEEDS010000004 DAEEDS010000000
VERSION     DAEEDS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75611
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.004"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(438..644)
                     /locus_tag="KLH32_000268"
     CDS             complement(438..644)
                     /locus_tag="KLH32_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBE0137260.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            957..1046
                     /gene="kdpF"
                     /locus_tag="KLH32_000269"
     CDS             957..1046
                     /gene="kdpF"
                     /locus_tag="KLH32_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBE0137261.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1046..2719
                     /gene="kdpA"
                     /locus_tag="KLH32_000270"
     CDS             1046..2719
                     /gene="kdpA"
                     /locus_tag="KLH32_000270"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBE0137262.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2742..4789
                     /gene="kdpB"
                     /locus_tag="KLH32_000271"
                     /pseudo
     CDS             2742..4789
                     /gene="kdpB"
                     /locus_tag="KLH32_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4798..5370
                     /gene="kdpC"
                     /locus_tag="KLH32_000272"
     CDS             4798..5370
                     /gene="kdpC"
                     /locus_tag="KLH32_000272"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBE0137263.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5363..8043
                     /gene="kdpD"
                     /locus_tag="KLH32_000273"
                     /pseudo
     CDS             5363..8043
                     /gene="kdpD"
                     /locus_tag="KLH32_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8040..8717
                     /gene="kdpE"
                     /locus_tag="KLH32_000274"
     CDS             8040..8717
                     /gene="kdpE"
                     /locus_tag="KLH32_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBE0137264.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8992..9096
                     /locus_tag="KLH32_000275"
     CDS             8992..9096
                     /locus_tag="KLH32_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBE0137265.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9407..11595
                     /gene="speF"
                     /locus_tag="KLH32_000276"
                     /pseudo
     CDS             9407..11595
                     /gene="speF"
                     /locus_tag="KLH32_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11592..12911
                     /gene="potE"
                     /locus_tag="KLH32_000277"
     CDS             11592..12911
                     /gene="potE"
                     /locus_tag="KLH32_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBE0137266.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12967..14607)
                     /gene="pgm"
                     /locus_tag="KLH32_000278"
     CDS             complement(12967..14607)
                     /gene="pgm"
                     /locus_tag="KLH32_000278"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBE0137267.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14633..15178)
                     /gene="seqA"
                     /locus_tag="KLH32_000279"
     CDS             complement(14633..15178)
                     /gene="seqA"
                     /locus_tag="KLH32_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBE0137268.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15363..16127
                     /gene="ybfF"
                     /locus_tag="KLH32_000280"
     CDS             15363..16127
                     /gene="ybfF"
                     /locus_tag="KLH32_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0137269.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16267..16560
                     /gene="ybfE"
                     /locus_tag="KLH32_000281"
     CDS             16267..16560
                     /gene="ybfE"
                     /locus_tag="KLH32_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBE0137270.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16700..17230
                     /gene="fldA"
                     /locus_tag="KLH32_000282"
     CDS             16700..17230
                     /gene="fldA"
                     /locus_tag="KLH32_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBE0137271.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17440..17526
                     /gene="uof"
                     /locus_tag="KLH32_000283"
     CDS             17440..17526
                     /gene="uof"
                     /locus_tag="KLH32_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBE0137272.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17519..17965
                     /gene="fur"
                     /locus_tag="KLH32_000284"
     CDS             17519..17965
                     /gene="fur"
                     /locus_tag="KLH32_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBE0137273.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18049..18375)
                     /locus_tag="KLH32_000285"
     CDS             complement(18049..18375)
                     /locus_tag="KLH32_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137274.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18412..19818)
                     /gene="chiP"
                     /locus_tag="KLH32_000286"
     CDS             complement(18412..19818)
                     /gene="chiP"
                     /locus_tag="KLH32_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBE0137275.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20397..22061)
                     /gene="glnS"
                     /locus_tag="KLH32_000287"
     CDS             complement(20397..22061)
                     /gene="glnS"
                     /locus_tag="KLH32_000287"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBE0137276.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22264..24210)
                     /gene="nagE"
                     /locus_tag="KLH32_000288"
     CDS             complement(22264..24210)
                     /gene="nagE"
                     /locus_tag="KLH32_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBE0137277.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24543..25343
                     /gene="nagB"
                     /locus_tag="KLH32_000289"
     CDS             24543..25343
                     /gene="nagB"
                     /locus_tag="KLH32_000289"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE0137278.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRLFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25403..26551
                     /gene="nagA"
                     /locus_tag="KLH32_000290"
     CDS             25403..26551
                     /gene="nagA"
                     /locus_tag="KLH32_000290"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE0137279.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26560..27780
                     /gene="nagC"
                     /locus_tag="KLH32_000291"
                     /gene_synonym="nagR"
     CDS             26560..27780
                     /gene="nagC"
                     /locus_tag="KLH32_000291"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBE0137280.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27828..28580
                     /gene="nagD"
                     /locus_tag="KLH32_000292"
     CDS             27828..28580
                     /gene="nagD"
                     /locus_tag="KLH32_000292"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBE0137281.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28977..30641
                     /gene="asnB"
                     /locus_tag="KLH32_000293"
     CDS             28977..30641
                     /gene="asnB"
                     /locus_tag="KLH32_000293"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBE0137282.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31020..31096
                     /locus_tag="KLH32_000294"
     tRNA            31020..31096
                     /locus_tag="KLH32_000294"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31054..31056,aa:Met,seq:cat)
     gene            31105..31189
                     /locus_tag="KLH32_000295"
     tRNA            31105..31189
                     /locus_tag="KLH32_000295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31139..31141,aa:Leu,seq:tag)
     gene            31213..31287
                     /locus_tag="KLH32_000296"
     tRNA            31213..31287
                     /locus_tag="KLH32_000296"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31245..31247,aa:Gln,seq:ttg)
     gene            31322..31396
                     /locus_tag="KLH32_000297"
     tRNA            31322..31396
                     /locus_tag="KLH32_000297"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31354..31356,aa:Gln,seq:ttg)
     gene            31412..31488
                     /locus_tag="KLH32_000298"
     tRNA            31412..31488
                     /locus_tag="KLH32_000298"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31446..31448,aa:Met,seq:cat)
     gene            31537..31611
                     /locus_tag="KLH32_000299"
     tRNA            31537..31611
                     /locus_tag="KLH32_000299"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31569..31571,aa:Gln,seq:ctg)
     gene            31649..31723
                     /locus_tag="KLH32_000300"
     tRNA            31649..31723
                     /locus_tag="KLH32_000300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31681..31683,aa:Gln,seq:ctg)
     gene            complement(31877..33052)
                     /gene="ubiF"
                     /locus_tag="KLH32_000301"
     CDS             complement(31877..33052)
                     /gene="ubiF"
                     /locus_tag="KLH32_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBE0137283.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33198..34622
                     /gene="miaB"
                     /locus_tag="KLH32_000302"
     CDS             33198..34622
                     /gene="miaB"
                     /locus_tag="KLH32_000302"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBE0137284.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34775..35815
                     /locus_tag="KLH32_000303"
     CDS             34775..35815
                     /locus_tag="KLH32_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBE0137285.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35812..36279
                     /gene="ybeY"
                     /locus_tag="KLH32_000304"
     CDS             35812..36279
                     /gene="ybeY"
                     /locus_tag="KLH32_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBE0137286.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36369..37247
                     /gene="corC"
                     /locus_tag="KLH32_000305"
     CDS             36369..37247
                     /gene="corC"
                     /locus_tag="KLH32_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBE0137287.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37272..38810
                     /gene="lnt"
                     /locus_tag="KLH32_000306"
     CDS             37272..38810
                     /gene="lnt"
                     /locus_tag="KLH32_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBE0137288.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39207..40115
                     /gene="gltI"
                     /locus_tag="KLH32_000307"
     CDS             39207..40115
                     /gene="gltI"
                     /locus_tag="KLH32_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBE0137289.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40285..41025
                     /gene="gltJ"
                     /locus_tag="KLH32_000308"
     CDS             40285..41025
                     /gene="gltJ"
                     /locus_tag="KLH32_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBE0137290.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41025..41699
                     /gene="gltK"
                     /locus_tag="KLH32_000309"
     CDS             41025..41699
                     /gene="gltK"
                     /locus_tag="KLH32_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBE0137291.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41699..42424
                     /gene="gltL"
                     /locus_tag="KLH32_000310"
     CDS             41699..42424
                     /gene="gltL"
                     /locus_tag="KLH32_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBE0137292.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42542..43477
                     /gene="rihA"
                     /locus_tag="KLH32_000311"
     CDS             42542..43477
                     /gene="rihA"
                     /locus_tag="KLH32_000311"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBE0137293.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43561..45222
                     /gene="hscC"
                     /locus_tag="KLH32_000312"
                     /pseudo
     CDS             43561..45222
                     /gene="hscC"
                     /locus_tag="KLH32_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45282..46733)
                     /gene="djlC"
                     /locus_tag="KLH32_000313"
     CDS             complement(45282..46733)
                     /gene="djlC"
                     /locus_tag="KLH32_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBE0137294.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46730..47437)
                     /locus_tag="KLH32_000314"
     CDS             complement(46730..47437)
                     /locus_tag="KLH32_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0137295.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47539..48093
                     /locus_tag="KLH32_000315"
     CDS             47539..48093
                     /locus_tag="KLH32_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE0137296.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48103..49530)
                     /locus_tag="KLH32_000316"
     CDS             complement(48103..49530)
                     /locus_tag="KLH32_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBE0137297.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49527..50234)
                     /locus_tag="KLH32_000317"
     CDS             complement(49527..50234)
                     /locus_tag="KLH32_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0137298.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50398..51375
                     /locus_tag="KLH32_000318"
     CDS             50398..51375
                     /locus_tag="KLH32_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE0137299.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51445..51927)
                     /locus_tag="KLH32_000319"
     CDS             complement(51445..51927)
                     /locus_tag="KLH32_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBE0137300.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52162..54744
                     /gene="leuS"
                     /locus_tag="KLH32_000320"
     CDS             52162..54744
                     /gene="leuS"
                     /locus_tag="KLH32_000320"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBE0137301.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54759..55340
                     /gene="lptE"
                     /locus_tag="KLH32_000321"
     CDS             54759..55340
                     /gene="lptE"
                     /locus_tag="KLH32_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBE0137302.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55340..56371
                     /gene="holA"
                     /locus_tag="KLH32_000322"
     CDS             55340..56371
                     /gene="holA"
                     /locus_tag="KLH32_000322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBE0137303.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56373..57014
                     /gene="nadD"
                     /locus_tag="KLH32_000323"
     CDS             56373..57014
                     /gene="nadD"
                     /locus_tag="KLH32_000323"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBE0137304.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57038..57649
                     /locus_tag="KLH32_000324"
     CDS             57038..57649
                     /locus_tag="KLH32_000324"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBE0137305.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57909..58226
                     /gene="rsfS"
                     /locus_tag="KLH32_000325"
                     /gene_synonym="rsfA"
     CDS             57909..58226
                     /gene="rsfS"
                     /locus_tag="KLH32_000325"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBE0137306.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58230..58697
                     /gene="rlmH"
                     /locus_tag="KLH32_000326"
     CDS             58230..58697
                     /gene="rlmH"
                     /locus_tag="KLH32_000326"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBE0137307.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58728..60629
                     /gene="mrdA"
                     /locus_tag="KLH32_000327"
     CDS             58728..60629
                     /gene="mrdA"
                     /locus_tag="KLH32_000327"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBE0137308.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60632..61744
                     /gene="mrdB"
                     /locus_tag="KLH32_000328"
                     /gene_synonym="rodA"
     CDS             60632..61744
                     /gene="mrdB"
                     /locus_tag="KLH32_000328"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBE0137309.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGMWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61755..62843
                     /gene="rlpA"
                     /locus_tag="KLH32_000329"
     CDS             61755..62843
                     /gene="rlpA"
                     /locus_tag="KLH32_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBE0137310.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62982..64193
                     /gene="dacA"
                     /locus_tag="KLH32_000330"
     CDS             62982..64193
                     /gene="dacA"
                     /locus_tag="KLH32_000330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBE0137311.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64305..64568
                     /locus_tag="KLH32_000331"
     CDS             64305..64568
                     /locus_tag="KLH32_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBE0137312.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64669..65310
                     /gene="lipB"
                     /locus_tag="KLH32_000332"
     CDS             64669..65310
                     /gene="lipB"
                     /locus_tag="KLH32_000332"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBE0137313.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65569..66522
                     /locus_tag="KLH32_000333"
     CDS             65569..66522
                     /locus_tag="KLH32_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE0137314.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66731..67696
                     /gene="lipA"
                     /locus_tag="KLH32_000334"
     CDS             66731..67696
                     /gene="lipA"
                     /locus_tag="KLH32_000334"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBE0137315.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67797..68000)
                     /gene="tatE"
                     /locus_tag="KLH32_000335"
     CDS             complement(67797..68000)
                     /gene="tatE"
                     /locus_tag="KLH32_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBE0137316.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68129..68917)
                     /locus_tag="KLH32_000336"
     CDS             complement(68129..68917)
                     /locus_tag="KLH32_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBE0137317.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69010..69393
                     /gene="crcB"
                     /locus_tag="KLH32_000337"
     CDS             69010..69393
                     /gene="crcB"
                     /locus_tag="KLH32_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBE0137318.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69447..69656)
                     /gene="cspE"
                     /locus_tag="KLH32_000338"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69447..69656)
                     /gene="cspE"
                     /locus_tag="KLH32_000338"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE0137319.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69831..70391)
                     /gene="pagP"
                     /locus_tag="KLH32_000339"
     CDS             complement(69831..70391)
                     /gene="pagP"
                     /locus_tag="KLH32_000339"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBE0137320.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70979..72364
                     /gene="dcuC"
                     /locus_tag="KLH32_000340"
     CDS             70979..72364
                     /gene="dcuC"
                     /locus_tag="KLH32_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE0137321.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72405..72746)
                     /locus_tag="KLH32_000341"
                     /pseudo
     CDS             complement(72405..72746)
                     /locus_tag="KLH32_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73084..73593)
                     /locus_tag="KLH32_000342"
     CDS             complement(73084..73593)
                     /locus_tag="KLH32_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0137322.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73756..74001
                     /locus_tag="KLH32_000343"
     CDS             73756..74001
                     /locus_tag="KLH32_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0137323.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73976..75607
                     /locus_tag="KLH32_000344"
                     /pseudo
     CDS             73976..75607
                     /locus_tag="KLH32_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18611 a        18987 c        19205 g        18808 t
ORIGIN      
        1 caacataaca tcttattgga ttagatatgt taacaattaa ttattcttaa tatatcaata
       61 aagtatatat tactctcttt actatctgtt tttattgcgg tagcgatact aataacaaaa
      121 ttcggaattt atttagatgc tggaattaga aaaagggatg acggctttct caccttaccc
      181 tgaagtttca cgaaagtgtg taaggaagta tgcaatctaa ctggagattt ctgatgatct
      241 agtcgcttag agcgacaatg aaggtgaccg catcctgggt agccctatga aaggatacta
      301 ttgaaacgtg atgctaaaac agtgatgaaa tgaataatac gatggaaacg tcttccatgt
      361 taattatcat ggaagacgtt gatgttgaac acctaaacca gccccctgat acgaggctgg
      421 cttaagcgcg caggcaatca gtaaaaatca ccggttgcct tttcggcctg atccatccac
      481 accggtttat cgctggtttt cgaccagacg cggtgcaggt agctgtaaaa gcgggcgcgg
      541 tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc atatgtacgg
      601 cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt ctcccccttt
      661 tgtatctacc cggtgaatag caccggaaaa atgaatttgt ttatctgatg aaaatagtac
      721 cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt gatggtttat
      781 ttacattcac actgtaatta aattacacaa aagttaaatt aatactaacc atcagttaaa
      841 tcatggcctt tgccattttt atactttttt tacaccccgt ccgcagattt ttgcgaaatc
      901 tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg tgcactgtga
      961 gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat ttggtttatg
     1021 ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt
     1081 tttactggta ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc ggctgattaa
     1141 tgacattcct cttccaggta cagcgggcgt tgaacgtata ctctttcgtt tacctggcgt
     1201 ctctgaccat gagatgaact ggaagcaata tctttgtgcc attctcggcc tgaacatgct
     1261 ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc ttaatccaca
     1321 gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct ttgtcacgaa
     1381 taccaactgg caatcttata gcggtgaaac cacgttgagc tatttcagcc agatggcagg
     1441 cttaacggta caaaactttc tttctgccgc cagcgggatt gcggtgattt ttgccttcat
     1501 ccgtgcgttt actcgccaga gcatgagcac actcgggaat gcctgggtcg atctgctacg
     1561 catcacgtta tgggtgctag tccctgtggc gttgttgatc gcactgtttt ttattcaaca
     1621 aggtgcgcag caaaactttc tgccctatca ggcagtgaat accgttgaag gagcgcaaca
     1681 gctgttaccc atgggaccag tagcttctca ggaagcgatc aagatgctcg gtactaacgg
     1741 cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg cactgaccaa
     1801 cttcgtgcag atgctggcga tcttcttgat cccaacagcg ctgtgctttg cctttggtga
     1861 agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt
     1921 catctgcgta ggcgtggtta tgtgggcaga agttcagggt aatcctcatc tgctggcgct
     1981 cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg tgctggtcag
     2041 tagcctgttt gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg cgatgcatga
     2101 ttcgtttacc gctctcggcg gcatggtgcc gatgtggcta atgcaaattg gtgaagtggt
     2161 gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc tggcggtgtt
     2221 tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tagacgtacg
     2281 cgagatgaaa ctgactgcac tggcgattct ggttactcca acactggtac tgatgggcgc
     2341 ggcgttggcg atgatgaccg acgccggacg tagcgccatg cttaacccta gcccgcatgg
     2401 ttttagcgaa gtgctgtacg ccgtgtcgtc cgccgctaac aacaacggca gcgcctttgc
     2461 cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg
     2521 tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag
     2581 ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg gcctgttaat
     2641 cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt
     2701 ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt aaacaactgg
     2761 cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc
     2821 cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca
     2881 cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg tttagcgcgg
     2941 caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag
     3001 aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact gcctttgccc
     3061 gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc gatcaactgc
     3121 gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat ggtgaagtca
     3181 ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc
     3241 gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt tctgactggc
     3301 tggtgattga gtgtagcgtt aaccccggtg agacatttct ggatcggatg atcgcgatgg
     3361 tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc ctgctgattg
     3421 ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg
     3481 gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc ccaaccacta
     3541 ttggtggcct gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg
     3601 tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt ctgctactgg
     3661 ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc cccgcgcagg
     3721 gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct gatgaaacgc
     3781 cggaaggtcg cagtattgtg atcctcgcca agcaacgttt taacctgcgc gagcgcgatg
     3841 tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg agcgggatca
     3901 acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catattgagg
     3961 ctaacggcgg tcacttccct gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcaag
     4021 gagccacgct gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt gcgctgaaag
     4081 atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaatgg gcattaaaac
     4141 ggtgatgatt accggcgata accgtctgac tgccgccgcg atagctgcgg aagcgggtgt
     4201 cgatgatttt ctcgccgaag cgacaccgga ggccaagctg gcattgatcc gtcagtatca
     4261 ggcggaaggt cgtttggtag cgatgaccgg cgacagcacc aacgatgcta cggcgctggc
     4321 gcaggcagat gtcgcggtgg cgatgaactc aggcacccag gcggctaaag aggcgggcaa
     4381 tatggtggat ctcgactcta acccgaccaa gttgatcgag gtggtgcaca ttggcaaaca
     4441 gatgctgatg acccgtggct cgctgaccac cttcagcatt gccaacgatg tggcgaaata
     4501 cttcgccatt attccggcgg cgttcgcggc aacatatccg cagttaaatg cgctgaacat
     4561 catgcgcctg cattcgcccg actccgccat tctcagtgcg gtcattttca acgccttgat
     4621 tatcgtcttt ttgattcctc tggcgttaaa aggcgtgagt tataaaccgc ttaccgcttc
     4681 tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg tgccgtttat
     4741 tggtatcaaa gtcattgatt tactgctaac cgtttgtagt ctggtgtgag gtttaccatg
     4801 agtggattac gtccggcatt atcgacattt ctctttctgt tattgattac tggcggcgtt
     4861 tacccgctgc tgaccaccgc actggggcaa tggtggtatc cctggcaggc caacggttcg
     4921 ttgattcgtg aaggtgatac ggtgcgcggt tcggcattaa tcgggcaaaa ttttaccggc
     4981 aacggctatt ttcatggtcg cccgtcggca acagcagaaa tgccctataa tccacaggct
     5041 tctggcggga gcaatctggc agtcagtaac ccggagctgg ataaacagat agccgcacgc
     5101 gttgccgcat tacgggccgc taacccggac gccagcacga acgttccggt tgaactggtg
     5161 acggcgtcgg caagcggact ggacaataac atcaccccgc aagcggcggc ctggcagatc
     5221 ccacgcatcg caaaagcgcg taatctcagc gttgaacagc tcacgcagct gatcgcaaaa
     5281 tacagccaac aaccgctggt gaaatatatc ggccagccgg tcgtcaacat tgtcgaactc
     5341 aatctggcac tggataagct tgatgaataa cgaaccctta cgtcccgacc ccgaccgtct
     5401 gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttca gtgcctgtgc
     5461 aggcgtcggg aagacctggg cgatgctggc agaagcccag cgactgcggg cgcaagggct
     5521 ggatattgtg gttggtgtgg tagaaaccca tgggcggaaa gataccgccg ccatgcttga
     5581 agggctggct gttctgcctc tgaaacgcca ggcgtaccgt gggcggcata tcagcgagtt
     5641 tgatctcgat gccgccctcg cccgccgtcc ggcgctgatc ttaatggacg aactggcgca
     5701 cagtaatgcg ccaggttctc gtcatcctaa acgctggcag gatatcgaag aactgctgga
     5761 agctggcatt gatgttttca caaccgtcaa cgttcagcat ctggaaagtc tgaatgatgt
     5821 ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc
     5881 cgccgacgac gtggtgctgg tggacttgcc cccggacgat ctgcgccagc ggctgaaaga
     5941 aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg
     6001 caatctgatc gccctgcgcg aactggcact gcgccgtact gccgatcgcg ttgatgagca
     6061 aatgcgcgcc tggcgggggc atcctggcga agagaaagtc tggcacacgc gcgacgcgat
     6121 ccttttatgc atcggccata acaccggcag cgaaaactgg tccgcgcggc ggcgcggctg
     6181 acgtcacggc tggggagcgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc
     6241 ttaccggaga aaaaacgtcg ggcaattctc agcgccttac gtctggcgca ggagctgggc
     6301 gcggagacgg caacactttc tgatccagcg gaagagaaag cggtagtgcg ttatgcccgt
     6361 gaacataatc tcggcaagat tattctcggt cgcccagcct cgcgccgctg gtggcgtcgg
     6421 gaaacatttg ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg
     6481 cttgatgaac cacccgcccg cacgattaac aacgcgccgg atagccgctc atttaaagac
     6541 aagtggcggg tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc
     6601 ttaattgcca tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatctg
     6661 cttggcgtgg tggtggcgct attttatgga cgctggcctt cggtggttgc caccgtcatt
     6721 aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat
     6781 gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt
     6841 actgctggcg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac
     6901 ttatatgaaa tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc
     6961 agcgaacaat ttattgcctc cacgtttcat gcccgcagtc aggtgttgtt gcccgatgac
     7021 aacggtaaat tgcagccgtt aacacatccg caaggagtta cgccatggga cgatgccatc
     7081 gcgcagtgga gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt
     7141 gtaccgtacc agattttgcc gctaaaaagc ggcgagaaaa cctacgggct ggtggtggtg
     7201 gagccgggga atctgcgcca gttgatgatc ccggaacagc agcgcctgct ggagactttt
     7261 acgctgttag tcgccaatgc ccttgagcgg ctgacgctaa ccgccagcga agaacaggcg
     7321 cggatggcaa gcgaacgtga acagatccgc aacgccctgc tggcagcgct ttcgcatgat
     7381 ttacgcacgc cgcttacggt gctgtttggt caggcagaaa tcttaacgct cgatctggca
     7441 agcgaaggat caccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacact
     7501 acccgactgg tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg
     7561 aagaaagagt ggttaacgct ggaagaagta gtcggcagcg cgctgcaaat gccggaaccg
     7621 ggtttatcgt cgcccatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac
     7681 gggctactct ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt
     7741 gcgcaggccg aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc
     7801 tgggataacg gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct
     7861 cgcgggaata aagagtcggc ggtgccgggt gtcgggcttg ggttggcaat ttgtcgggcg
     7921 attgtggatg tacacggcgg caccattacc gcgttcaacc gaccggaagg tggtgcctgt
     7981 tttcgtgtta cacttcccca gcaaaccgct cctgaacttg aagactttca tgaggatatg
     8041 tgacaaacgt tctgattgtt gaagatgaac aggctattcg tcgctttctg cgcacggcgc
     8101 tggagggcga cgggatgcgc gtctttgagg ccgaaactct gcaacgcggc ttgctggaag
     8161 cggcaacccg taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga
     8221 ttgagtttat ccgcgacctg cgccagtgga gcccggtgcc ggtgattgtg ctttccgcac
     8281 gcagcgaaga gagcgacaag atcgccgcgc tggatgccgg agcggatgat tatctgagta
     8341 agccgtttgg tattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg
     8401 ccaccgccgc gcccgatccg ctggtgaaat tttccggtgt taccgtcgat ttagccgccc
     8461 gcgtgattca ccggggtgag gaagaggtgc atctcacacc aattgagttc cgcctgctgg
     8521 cggtgctgct caacaatgcc ggaaaagtac tcacccagcg ccagctcctt aaccaggtgt
     8581 gggggccaaa cgcggtcgaa cacagtcact atttgcgtat ttatatggga catctgcgac
     8641 aaaaactgga acaggatccg gcccggcctc gccacttcat tactgaaacc ggtattgggt
     8701 atcggtttat gctttgaatt gttattcaat aaattcgctt aaatagtgat taatttatta
     8761 ttaaacctgc gccgaatatt attatttggc aggtttaatt tcttaacgca aatataaaac
     8821 acgaaattac aacatttaag taacaaaaca gcatctaata tggcgatatt cataattaat
     8881 gatttattat ctggggtatg atcgtttttt gttgatcttc ttcacagatt atagccattt
     8941 catggataga ataactctac cttcaactga cacagcaagc ggtaaaggta aatggaaaat
     9001 aataaccgct taatgcctca tataaggcgg acaacccata tcatgaagtt tgcccatcgt
     9061 aatagttttg actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccaa
     9121 cgtacaggtc tccctgactt taagcattac aggttaatac ctgtattcct cggtgctcat
     9181 atactgctac cccttttaaa ctctaaataa ttcgagtcgc agcacttgca acttgaggta
     9241 tgacgagtat agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta
     9301 ctgatttatt tatcagcggt ggagctttgc tttttttccg acgtgatcga tttctccttt
     9361 gagaaattga ggacctgcta ttacctgaaa taaagagatg aataaaatgt cagaattaaa
     9421 aattgccgtt agccgttctt gcccggattg tttttccact catcgtgcat gcgtgaatat
     9481 tgacgaaagt aattatattg acgttgccgc cattatttta tcagtcaatg atgttgaacg
     9541 tggaaaactc gataaaatag acgctactgg ctatggcatt cctgttttta ttgcaacgga
     9601 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
     9661 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
     9721 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
     9781 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
     9841 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
     9901 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
     9961 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
    10021 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
    10081 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
    10141 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
    10201 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
    10261 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
    10321 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
    10381 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
    10441 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
    10501 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
    10561 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
    10621 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
    10681 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
    10741 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
    10801 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
    10861 agagaatcaa tactttgtcg atccttgcaa actgttgctg acaaccccag gtattgatgc
    10921 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
    10981 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
    11041 ggcagaagat atggccaaac ttcagcaact tgtgccctgc tggtacgctt cgaaaaactg
    11101 cttgagtccg acgcgccatt agcagaagtg ctgccttcca tctacaaaca gcatgaagag
    11161 cgctacgccg gttataccct gcgtcagttg tgccaggaaa tgcatgattt gtatgcccgc
    11221 cacaacgtga aacaactgca aaaagagatg ttccgcaaag cgcacttccc acacgtcagc
    11281 atgaatccgc aagaagccaa ctacgcctat ttacgcggtg aagtggagct ggttcgtctg
    11341 ccggatgccg aaggtcgtat cgctgccgaa ggggcgcttc cttatcctcc gggtgtgctg
    11401 tgtgttgttc cgggtgaaat ctggggtggt gctgttttgc gtgctctgga agaagggatc
    11461 aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga acatgatggt
    11521 cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac cctgttgaaa
    11581 ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag ttaaccatac
    11641 tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag cttgccgaag
    11701 ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg gcactggcat
    11761 gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc ggttatgccg
    11821 agtatgcttt cggtaaatcc ggtaacttta ttgcgaacta tacctacggc gtctcactgc
    11881 tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa ctgctcggcg
    11941 cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgctgtgg atttgtaccg
    12001 tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc gtgtgggggg
    12061 gcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc ccgacgcttt
    12121 acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt tcttccatcg
    12181 ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact gatgtagtgg
    12241 aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt gcggcggtga
    12301 tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag ctggcaaatt
    12361 caacggcacc atttggcctg gccttcgcac agatgtttac accagaagtg ggtaaagtca
    12421 ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag ttcaccattg
    12481 cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc tcccgtgtaa
    12541 ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag agcgggttgg
    12601 cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt aacctggccg
    12661 tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt attcagaagg
    12721 tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt gttggcgcga
    12781 tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac ggttccatcg
    12841 tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa ctgaaaaata
    12901 aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgctctt tttttattaa
    12961 atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt tctcaatctg
    13021 cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc
    13081 tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc caatagaagc
    13141 accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca gggtgctggc
    13201 gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag tcgcagctgc
    13261 ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg
    13321 cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa tgatgccgtc
    13381 tttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc
    13441 gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga aacctaccgg
    13501 tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg cagaagaaac
    13561 cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga acaggtagtt
    13621 gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga cgataccgtg
    13681 acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca gtgccagcag
    13741 acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt tatcgaggtg
    13801 cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga ggttgtaata
    13861 ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa cgcccagcgt
    13921 caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt ccacgaacgg
    13981 ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga gggagatacg
    14041 cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca ccactttggt
    14101 gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac catcttccgg
    14161 cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt ttttattgtg
    14221 aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt cctgcacaat
    14281 gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag gctcggacag
    14341 ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg cacgttcttc
    14401 agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc gcgctgcact
    14461 gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg cttctggttt
    14521 cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct gttgtgcagg
    14581 ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg ttttagatag
    14641 ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg atgtgttcga
    14701 tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc gtgcctggca
    14761 catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt
    14821 acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag
    14881 aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc tctgcgtatt
    14941 cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct
    15001 tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct
    15061 gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat
    15121 gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc gttttcatct
    15181 taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa taaatcccgg
    15241 cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta tttgttgcag
    15301 ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa aagaactttg
    15361 ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat tctcccatcg
    15421 ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct cgcgatctgg
    15481 taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca ccgagagagc
    15541 cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat gcactgcaga
    15601 tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg gcacttactg
    15661 cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg gtcgactatc
    15721 acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa tcggacgcac
    15781 aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa ggggtgattc
    15841 agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtgccg gtattgtggg
    15901 atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac cctgccctgt
    15961 ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat ttactggcac
    16021 aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc catgctgaaa
    16081 aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc aactgattaa
    16141 aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat cgcgctgatg
    16201 tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc cgaagtcacc
    16261 aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc gcacgagcgt
    16321 cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg tattaataaa
    16381 cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact gaagctgaac
    16441 gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag ccgtagcgaa
    16501 ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa
    16561 atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg
    16621 cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt tcaataagtt
    16681 tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg acaccggtaa
    16741 taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg ccgatgtcca
    16801 tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc tgctgggcat
    16861 cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc cgactctcga
    16921 agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc aggaagatta
    16981 cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac cgcgcggtgc
    17041 aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa aaggtctggc
    17101 agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg aactgaccgc
    17161 tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg acgaaattct
    17221 taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag ataatgccaa
    17281 tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat gtcccagtgt
    17341 tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac aatatttgcc
    17401 agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat tatctcaaga
    17461 gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca gattccgcat
    17521 gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa
    17581 aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag atttatacaa
    17641 acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg tactgaacca
    17701 gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat ccgtatttga
    17761 actgacacag caacatcacc acgatcacct gatttgcctc gactgcggca aggttatcga
    17821 atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg
    17881 cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga
    17941 tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac ctgcgggttg
    18001 gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt
    18061 actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat catttccggt
    18121 ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct ggtcggcaaa
    18181 ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt
    18241 ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca gacggctgtc
    18301 ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca tggcgatgag
    18361 aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc atcagaagat
    18421 ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga tgttaccgta
    18481 accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt gcagtttgaa
    18541 catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc tgtaggcaga
    18601 ctcttcaata gtccggtttt tgtcgtagta cgcatccgga ttgctctgcc aggtcgcagg
    18661 tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa gattccagtt
    18721 tttcaggtca tacatcgcac tgaagaagac cgctttttcg ccgttagcgt tgaagtcgga
    18781 acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg gggtcatacg
    18841 ttgcaggaag tatccctgct gactgtcagc cttaatccag gtgccttcga ggcgcaaatc
    18901 cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaggcgg tgccgtcata
    18961 aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact ggtagctggt
    19021 ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat attgatcgat
    19081 atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga agtcgtattt
    19141 cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat aaaactcatc
    19201 catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga aactcaaggc
    19261 accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag tacccggcat
    19321 aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac ctgccctcgc
    19381 ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac cgcttttgtc
    19441 gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt cgttcgggtg
    19501 ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat caaggccgaa
    19561 catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg agtgagaaag
    19621 gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac gctgccagta
    19681 atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag cctccggcat
    19741 tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg tactacgttt
    19801 gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc accaaaggtg
    19861 aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg ctgactacag
    19921 gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag aatgtaagaa
    19981 catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt gcggttgaaa
    20041 ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta cattttattt
    20101 aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg aattaattcg
    20161 cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg ctatttcttg
    20221 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca atatattgca
    20281 gagatcatgt aggcctgata agcgaagcgc atcaggcaat ttagcgtttg aaatttattg
    20341 tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac ttaaaattac
    20401 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcg
    20461 gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta
    20521 cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc
    20581 gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct gaacagacga
    20641 tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
    20701 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
    20761 atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca
    20821 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
    20881 cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg gctccccatt
    20941 tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg
    21001 ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gcgcattttc gttgagatct
    21061 tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt ggtcacgccg
    21121 atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg cagaccggaa
    21181 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
    21241 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
    21301 ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg gttgtcctgg
    21361 aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat gcaatgggtg
    21421 aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta
    21481 atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc gattttcgca
    21541 cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccaag
    21601 ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt cagggtaccg
    21661 cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc tttattgatc
    21721 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
    21781 gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata ctcgatatct
    21841 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
    21901 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
    21961 ggcgggaaac gggtgtgtac tgtgatgtgc ttaccactgg ccagatcttc atcgatgatc
    22021 tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt
    22081 aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt
    22141 taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga
    22201 agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca ggcataaagc
    22261 agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt
    22321 aatgagcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg
    22381 ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg
    22441 cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc
    22501 catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt
    22561 gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac
    22621 cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc
    22681 cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc
    22741 ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg cagcggctac
    22801 cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac
    22861 aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca
    22921 catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg taatacaggc
    22981 atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg ttgccagttg
    23041 agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt cttctttatc
    23101 ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta
    23161 gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc tggcggcagg
    23221 caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc cggcatggat
    23281 acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag
    23341 cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc
    23401 agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc
    23461 gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga agaagccgga
    23521 catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc cgtggaaaac
    23581 cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg
    23641 atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc
    23701 gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg gcggccagac
    23761 gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg gcacaaagcg
    23821 tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc
    23881 tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt
    23941 gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc
    24001 acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt
    24061 atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc
    24121 gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta acgccctacc
    24181 gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc
    24241 tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc tgtaatcaga
    24301 ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgcatcgcaa attaaacgcg
    24361 tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac
    24421 cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc tgcgcaaatc
    24481 caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta ttgaggtgaa
    24541 taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata
    24601 tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tctgtttgta ctgggcctgc
    24661 cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc
    24721 aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag
    24781 agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc
    24841 cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc gagtgccgcc
    24901 agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta
    24961 acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca
    25021 aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc
    25081 aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc gaagaagtga
    25141 tgattatggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt gaaggttgcg
    25201 tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc atggtgtgcg
    25261 atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag
    25321 cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct cagggcaata
    25381 ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca
    25441 cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc
    25501 ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc
    25561 cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga
    25621 agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac
    25681 taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt
    25741 tatgcgtgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc
    25801 gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc
    25861 cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc
    25921 ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc
    25981 cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt
    26041 tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg
    26101 cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga
    26161 ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga
    26221 cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata
    26281 ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat
    26341 gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg
    26401 tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg gcacgctcgc
    26461 cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat
    26521 cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct
    26581 cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt
    26641 gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc
    26701 agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag
    26761 caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac
    26821 gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga tctcagcagc
    26881 aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac gctggaacat
    26941 gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct gcgcgagcta
    27001 atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac
    27061 atgccgcata ttcaggtaga aaactggggg ctggtagaag cactggaaga acgttttaaa
    27121 gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt
    27181 gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg
    27241 attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat
    27301 attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact
    27361 atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag
    27421 agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc
    27481 gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa aaccatcgcc
    27541 attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat caccgaagcc
    27601 gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct gaaggcgttt
    27661 cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt
    27721 gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa
    27781 tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa
    27841 atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag
    27901 cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc
    27961 cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg
    28021 atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc caggaaggca
    28081 agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca
    28141 ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc tacaactggg
    28201 acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc
    28261 cggacaccca cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
    28321 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg
    28381 cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta
    28441 ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt
    28501 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
    28561 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca
    28621 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    28681 aattttcatg cttttgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaatc
    28741 gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa
    28801 ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca
    28861 ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca
    28921 tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
    28981 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    29041 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    29101 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    29161 ctctctacaa ccaacaaaaa aaccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    29221 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    29281 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    29341 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    29401 ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa
    29461 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    29521 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    29581 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
    29641 gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa
    29701 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    29761 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    29821 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    29881 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    29941 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    30001 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    30061 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccttgc
    30121 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    30181 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    30241 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    30301 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    30361 tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc
    30421 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct
    30481 ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg
    30541 cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt
    30601 ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg
    30661 gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga
    30721 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    30781 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    30841 gctgtagggt ggggaaaaac acaaagtaaa caataattga cgaatatagc gccacgctgt
    30901 tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag gttgacgctg
    30961 caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga aaaaaagatg
    31021 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    31081 cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc accagattta
    31141 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    31201 gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    31261 ctggttcgaa tccaggtacc ccagccatct tcttcgagta agcggttcac cgcccggtta
    31321 ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    31381 ccaggtaccc cagccatcga agaaataatc tggctacgta gctcagttgg ttagagcaca
    31441 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt
    31501 gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc
    31561 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc aatttattca
    31621 agacgcttac atcgtaagtg cacccagttg gggtatcgcc aagcggtaag gcaccggatt
    31681 ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccacattaaa aaagctcgct
    31741 tcggcgagct ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt
    31801 atccttcaat gtcggatgcg acgctgccgc gtcttatccg acctacggct ggcacgcatc
    31861 cggcaatgtt gtaaggctac aaccctaacg catatttcag cgcctgacgt ttcaacacgc
    31921 cagcacgctc tgccgccatt aacccgagat tacgcacaaa acgcagcggc ggcagattat
    31981 tactgaatcc ggcataaaac agatccatac cactttgcat aatgaagtta tccgccatgc
    32041 gccgcatctg gtaacgcttg aggacaggat aactggccca cgcttcaccg tagctgcggg
    32101 cattcaccag aacatcaatc agggcatcga catcacgata accaagattc accccctgcc
    32161 ccgccagcgg atggatggta tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat
    32221 actgcaacgc atgtcggcgc gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac
    32281 ccagacgcga cgggaaatgc ttcgcgattt ccgcctggag ttgcgccata ttcatattct
    32341 gcaactggcg aatacgcgcc ggagagtcat accacaccag cgatgcccag ttatcaaaca
    32401 acggcagaaa cgcacgcggt ccgtccgggg taaattgctg ccaggtgctg tcgccgggat
    32461 cgttctcaca ctgtacgcta atcaacatgc acgactgcgc atactgccag gcatgaacgc
    32521 caattcccgc catctgccgc acctgagaat ttgcgccgtc ggcaccaatc accagtttcg
    32581 cgcggatcgt ttcaccgccc ttcagttcca gctcctgaag atcattatct cgatgtaatg
    32641 caatcagcga ggttggcaca cgtaacgtta ctttcggatg cgcttccagc gcctgccaca
    32701 acgcctgttg caggactgtg ttttccacca tgtagccaag caacggcaac tttaattcgg
    32761 ctgcgtcaaa taccacatgt gccgtttccc actcccaggt ttccagtctg cggtaaggat
    32821 ggcaacgcat agcctgtact gcatcccaga cccctaaccc tttaagcaat gataccgaag
    32881 ccgcgctgat cgccgagatc cgcacgtccg gttggctgtc agcgacaaac ggtgcaggtt
    32941 ctgcgtgctc gatcaccgtt accgaaaatc cgtgctgtgc cagccccagc gccagtgcgc
    33001 cgccgaccat tcctccgccg acaatggcaa tttccgttgg ttgatttgtc atggtcaatc
    33061 atcctgttag caatatgctt taagtttaca ggatttttac ccccaagggc tgataacgct
    33121 catactggtc acaacggcag caaagcatta cactatgcgc cctgcattcc tggctactat
    33181 ttcgcaagag caagtcgatg accaaaaaac tccatattaa aacctggggc tgtcagatga
    33241 acgagtacga ttcatcgaag atggccgatc tgctggatgc cacccacggc tatcaactga
    33301 ccgacgtggc ggaagaagcg gatgtgctac tgctgaacac ctgctcaatc cgcgagaagg
    33361 ctcaggaaaa agtcttccat cagttgggtc gctggaaact gttaaaagag aagaatccag
    33421 acctgattat cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac attcgccagc
    33481 gcgcccacta tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg ccggagatga
    33541 tcaactccgt gcgcggcgac cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga
    33601 agtttgaccg tctgccggaa ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg
    33661 aaggctgcaa taaatattgc acctactgcg tggtgcctta cacccgtggt gaagaggtaa
    33721 gccgtccgtc tgacgatatt ctgtttgaga ttgcccagct tgcggctcag ggcgtgcgtg
    33781 aagtcaacct gctcggtcag aatgtgaatg cctggcgtgg tgagaactac gacggcacca
    33841 ccggatcgtt tgccgatctg ttgcgtctgg ttgctgcgat cgacgggatc gatcgtattc
    33901 gctttaccac cagccatccg atcgaattca ccgacgatat catcgaagtg taccgcgaca
    33961 cgcctgagct ggtgagcttc ctgcatctgc cggtacagag cggttccgat cgcattctga
    34021 acctgatggg acgtacccat acggcgctgg agtacaaagc gatcatccgt aaactgcgtg
    34081 cggcgcgtcc ggatattcag atcagttctg acttcattgt tggcttccct ggcgaaacca
    34141 ccgaagactt cgagaaaacg atgaagctga ttgccgacgt caatttcgac atgagctaca
    34201 gctttatctt ctctgcacgt ccaggaacgc ctgccgccga tatggttgat gatgttccgg
    34261 aagaagagaa gaagcagcgt ctgtatattc tgcaagaacg tatcaatcag caagcgatgg
    34321 catggagccg tcggatgctc ggcaccaccc agcgtattct ggtagaaggg acttcgcgta
    34381 agagcatcat ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca
    34441 caccggatat gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac ccgaactctc
    34501 tgcgcggtaa agtggtgcga actgaagatg aaatgggtct gcgcatggca gaaacaccgg
    34561 agtcggtgat tgcccgcacc cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt
    34621 aattctcagg cctgccgttc cggtgggcct tgttttttct ctctttatcc ccatctttat
    34681 cgcaatgctt gcgccttttt cctgtggcgt gaataatttc cttataggcc ggtgaatgtt
    34741 cagcttccgg cggcatacag ataagaggaa cggtttgaac atagacactc gcgaaatcac
    34801 cctggagcca gcagacaatg cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat
    34861 caagcagctc gaacgccgtc tcggcatcga gatcaatcgc cgcgataacc actttaaact
    34921 gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga
    34981 tactgccccg atgcgcggtc agattcagga tatcgaaccg gaacagatcc accttgcgat
    35041 taaagaagcg cgggtactgg agcaaagcgc ggagagcgtg ccggagtacg gcaaagcggt
    35101 caatatcaaa actaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat
    35161 cgccaatatt ctcgaccatg acattacctt cggcgttggc ccggcgggta cgggtaaaac
    35221 ctacctggca gtggctgcgg cagttgatgc cctggagcgt caggaaattc gccgtattct
    35281 gctgactcgt ccggcggtcg aagccggtga gaaactgggc ttcctgcctg gcgatttaag
    35341 ccagaaagta gacccgtatc tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt
    35401 tgagaaagtc gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat
    35461 gcgtggtcgt acgctgaacg acgcgtttat cattctcgat gagagccaga acactaccat
    35521 cgaacagatg aagatgttcc tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg
    35581 cgacgtcaca cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc atgccattga
    35641 agtgctggcc gatgtcgaag agatcagctt taacttcttc cacagcgaag acgtggttcg
    35701 tcacccggtg gtggcgcgta tcgttaacgc ttatgaagcc tgggaagaag cagaacaaaa
    35761 acgtaaagcg gcgctggcag cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag
    35821 gtgatcctcg atttacaact ggcatgtgaa gataattccg ggttaccgga agagagccag
    35881 tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg aagagtcgga agtgactatt
    35941 cgcgtggtcg ataccgccga aagccacagt ctgaatctga cctatcgcgg taaggataag
    36001 ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg gcatggagat gtcgctactg
    36061 ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag ctcaggagca aggcaaacca
    36121 ctggaggcgc attgggcgca tatggtggtg cacggcagtc tgcatttgtt aggttacgat
    36181 cacattgaag atgacgaagc agaagaaatg gaagccctcg aaacagagat tatgcttgct
    36241 ctgggctatg aggatccgta cattgccgag aaagaataat ttgccaggca tttgactggc
    36301 agacgtcccc gccgttggtg attaacgacg gcgttttgat taactaacat gagaacccat
    36361 acgacgccat gagcgacgac aattcacaca gtagtgacac gataagcaac aagaagggat
    36421 ttttctccct gttactcagc caacttttcc acggtgaacc gaaaaaccgt gacgaactgc
    36481 tggcgctgat ccgtgattcc gggcagaacg accttatcga cgaagatacg cgcgatatgc
    36541 tcgaaggggt gatggacatc gcagaccaac gcgttcgcga catcatgatc ccccgctccc
    36601 agatgattac cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc atcatcgagt
    36661 ccgcccactc acgtttcccg gtgattagcg aagacaaaga tcacattgaa gggattctga
    36721 tggcgaaaga cttgctgccg tttatgcgca gcgatgctga agccttcagc atggacaaag
    36781 tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt
    36841 ttcgctctca gcgttaccac atggcgatcg ttattgacga attcggtggg gtttccggtc
    36901 tggtgaccat tgaagacatc ctggaactga ttgttggtga gattgaagac gagtatgacg
    36961 aagaagatga tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg cgcgcactgg
    37021 cttccattga agacttcaac gaagcgttcg gcacccactt tagcgatgaa gaagtcgaca
    37081 ctatcggtgg tctggtgatg caggcgtttg ggcatcttcc ggcgcgtggc gaaactatcg
    37141 acatcgacgg ttaccagttc aaagtggcga tggccgatag tcggcgtatt attcaggttc
    37201 atgtcaaaat cccggatgac tcaccccagc caaagctgga tgaataaaac cgaaactgga
    37261 tagataacta catggctttt gcctcattaa ttgaacgcca gcgtattcgc ctgctgctgg
    37321 cgttattatt cggtgcctgc ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg
    37381 cgattatttc gctgatgggg cttcaggcgt tgacctttaa ccgtcgccca ctccagtctg
    37441 ccgctattgg cttttgctgg ggatttggcc tctttggcag cggtattaac tgggtctatg
    37501 tcagcatcgc gacctttggc gggatgcctg gcccggttaa catcttcctg gtggtgctgc
    37561 tggcggcgta tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc
    37621 cgaaaaccac ctggctgcgc gtggcgattg ccacccctgc tctctggcaa gtgaccgagt
    37681 ttctgcgcgg ttgggtgctg actggcttcc cgtggttaca gttcggctat agccagattg
    37741 atggcccgtt aaaagggctg gcaccgctaa tgggcgtgga agccattaac ttcctgctga
    37801 tgatggttag tggcctgctg gcactggcgt tggtcaaacg caactggcgt ccgctggtgg
    37861 tggccgtcgt gctgtttgcc ctacccttcc cgctgcgtta catccaatgg tttaccccgc
    37921 aaccggagaa aaccattcag gtttcgatgg ttcagggcga tattccgcaa tcgctgaaat
    37981 gggacgaagg ccagcttctt aatacgctaa agatttacta caacgcaacg gcaccgctga
    38041 tgggcaaatc atcgttgatt atctggccgg agtcggcgat aaccgatctg gaaattaatc
    38101 agcaaccgtt cctcaaagca ctggacggtg agttgcgtga taaaggtagc tcgctggtaa
    38161 ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta cgatacctac aacaccatca
    38221 tcacgctggg taaaggtgca ccgtacagct acgaatcagc cgatcgctat aacaaaaacc
    38281 atctggtgcc gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt
    38341 tctttgatct gccgatgtcg tcgttcagcc gtgggccata tatccagccg ccgctgtcgg
    38401 caaatggtat tgagcttact gcggctattt gctacgagat cattctcgcc gagcaagtgc
    38461 gcgataactt ccgcccggat accgactacc tgctgactat ctccaacgat gcgtggtttg
    38521 gtaaatctat tggtccatgg caacacttcc agatggcgcg aatgcgtgcg ctggagctgg
    38581 cgcgcccact gttgcgcagc accaacaacg gcattacggc ggtgattggc ccgcagggtg
    38641 agattcaggc gatgatcccg cagttcgccc gcgaggtgtt aaccactaac gtgacgccga
    38701 ccaccggact cacaccatac gcacgtaccg gcaactggcc gctgtgggtg ctgacggcac
    38761 tgtttggttt tgctgctgtg ttgatgagtc tgcgtcagcg acgtaaataa tcccttctta
    38821 tcgtgccgga atacatccca tatttcggca cgctcaccgg atgcggcatc agtgcattat
    38881 ccggataaca atattctcct atccaacttt ggcacatcta ttgctttgtt atacaaggca
    38941 aagcttgaac cagcatcagt acaacccggt tgcaccacag caatccaccg gtagcaccaa
    39001 aagagtgcat tttaagaatt ttgcttcaga atggtgcggt gcacctcgca aaaacaaaca
    39061 ataaaacatc aatatcttta catttagcca aactaaatgt tacctaacag gcacaacact
    39121 gcacaataaa gttgcagacg ataacaacac aaacactcac aacgggtatc catgcgttct
    39181 taacgcagaa gataaaggtg ttggatatgc aattacgtaa acctgccaca gcaatcctcg
    39241 ccctggcgct ttccgcagga ctggcacagg cagatgacgc cgccccggca gcaggcagta
    39301 cgctggacaa aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtac
    39361 ctttctctta ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg
    39421 ccattgttga agcagtgaaa aagaaactca acaaaccgga cttgcaggta aaactgattc
    39481 cgattacctc acaaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg
    39541 gttctaccac caacaacgtc gaacgccaaa aacaggcggc tttctctgac actattttcg
    39601 tggtcggtac gcgcctgttg accaaaaagg gcggcgatat caaagatttt gccgacctga
    39661 aaggcaaagc cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga
    39721 atgaagagca aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc
    39781 gcaccctgga aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg
    39841 aacgtgcgaa agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg
    39901 aagcctacgg ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca
    39961 ctatcgctca ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa
    40021 atccaattcc gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac
    40081 tgttcaaaga accgaatgac aaggcactga actaattaca agaaccaggg gcggaaattc
    40141 cagccctctc gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc
    40201 gggcctgaaa accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta
    40261 ccctcttttt tctggagtag atttatgtct atagactgga actggggtat ttttttacaa
    40321 caagccccgt tcggcaatac cacctatctt ggttgggtct ggagtggttt tcaggtgacg
    40381 atcgctttgt cgatctgcgc ctggattatc gctttcctcg tcggttcatt tttcggcatt
    40441 ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc
    40501 cgcaacgtgc cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg
    40561 ccggagaaaa tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt
    40621 tcatccatgc tctgcctggg gctgtttacc gccgcccgtg tttgcgaaca ggtacgcgcc
    40681 gctattcagt cgctaccgcg tgggcagaag aatgccgcgc tggcgatggg cctgacgcta
    40741 ccgcagactt atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg
    40801 acttccgaga tgatgaacct ggtgaaaaac tccgccatcg cctcgaccat cggtctggtg
    40861 gatatggcgg cgcaggcggg taaattgctc gactactcgg ctcacgcctg ggagtcgttt
    40921 accgccatca cgcttgcgta tgtgttgatt aacgctttta tcatgctggt gatgacgctg
    40981 gttgaacgta aagttcgcct gcccggcaac atggggggca aataatgtac gagtttgact
    41041 ggagttccat tgttccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa
    41101 tcaccgtcac ggcagtcgtg attggtattt tgtggggtac catgctggcg gtgatgcgct
    41161 tgtccagctt cgcgcctgtt tcgtggtttg ccaaagccta cgttaacgta ttccgctcga
    41221 ttcctttagt catggttttg ctgtggtttt acttgattgt gccgggattt ctgcaaaatg
    41281 tgctgggatt atcgccaaaa aatgatattc gtttaatttc ggcgatggtg gcgttttcga
    41341 tgtttgaagc ggcctactat tcagagatta tccgtgccgg tattcaaagt atttcccgtg
    41401 gtcagtcgag cgccgcgctg gcgttgggga tgactcactg gcagtcgatg aaactgatta
    41461 ttctgccgca ggctttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt
    41521 tccaggatac ctcgctggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa
    41581 ccattggtga gcgtgatggt acgcaggtcg agatgatcct gtttgccgga tttgtttatt
    41641 tcgttattag ccttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat
    41701 gattaccctg aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc
    41761 aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc
    41821 aacgctgatt aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga
    41881 tggtatcgtg gttaacgaca agaaaaccga tctggcaaag ctgcgctccc gcgtcgggat
    41941 ggtattccag catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc
    42001 gcaggtgaaa gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat tgaaattgct
    42061 ggagcgcgtg ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca
    42121 gcagcagcgc gtggcaatcg ctcgcgcatt gtgtatggct cctattgcga tgctgttcga
    42181 cgaaccgaca tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga
    42241 actggcgaac gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa
    42301 agtggcgaat cgggtgatct ttatggacga gggtaaaatt gtcgaagact cgccgaaaga
    42361 cgctttcttc gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca
    42421 ttaatcactc tggcgcacat acccaattgc gcgccatctc acgctttatc gtttcgcccc
    42481 tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac
    42541 aatggcactg ccaattctgt tagattgcga cccaggtcat gatgacgcta tcgcaatagt
    42601 tctcgcactc gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca
    42661 gacaccagaa aaaaccttac gcaatgttct gcgtatgctg accttgctta atcgcaccga
    42721 tattccggtg gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa
    42781 tgtgcacggc gaaagcggtc tcgacggccc ggcattaccg gaaccgacgt tcgcaccgca
    42841 aaactgtacg gcggtggagc tgatggcgaa aacgctgcgc gaaagtgcgg aacctgtcac
    42901 gattgtttct accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact
    42961 gcatagcaaa attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac
    43021 gcctgcggct gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc
    43081 agggatcccg gtggtgatgg ccggtctgga tgttactcat aaagcgcaaa tccacgttga
    43141 agacaccgag cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct
    43201 ggatttcttc ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca
    43261 tgacccatgc accatcgcct ggctgttgaa acccgagttg tttaccactg ttgagcgctg
    43321 ggttggcgtg gaaacacagg ggaaatatac ccagggtatg acggttgttg attattatta
    43381 tctgactggc aataaaccga atgccactgt aattgtcgat gttgatcgtc agggctttgt
    43441 tgatttactg gctgatcgtc tgaaatttta cgcttaacac tcgtcattga ttaagatcac
    43501 cacgcgttac ctgggtggtc ttaatctatt tatctctttt tctttcaaga tataaatgca
    43561 atggataatg cagaactcgc cataggtatc gatctcggca ctaccaatag tttaattgcc
    43621 gtctggaaag acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca
    43681 tccataatta gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg
    43741 cgtacttcgc atccggataa aacggcagcg ttatttaaac gtgcaatggg cagctggcgg
    43801 ttaggcagcg acacatttaa cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa
    43861 gaagatgccg aagaatttct gcaacgtccg attaaagatg tggtgatctc cgttccggct
    43921 tatttcagtg atgaacaacg caagcatacc cgtttagcag cggacttagc cgggttaaat
    43981 gcggtacgct taattaatga acccacagca gctgcgatgg cgtatggcct gcatacccaa
    44041 caaaataccc gttcgctggt gtttgatctc ggtggcggca cgtttgacgt tacggtgctt
    44101 gagtacgcca cgccggtgat tgaagttcac gcctccgctg gcgacaactt cctcggtggc
    44161 gaagatttta cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt cacaaggacc
    44221 acgcttaacg agagtgaact ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc
    44281 aatcaatcgc caatgcacat tcgctggtag taccaggatg aaacgcggga atgcgaattt
    44341 tacgagaacg aactggaaga tttgtggttg ccgctgctca atcgcttgcg agtgccgatt
    44401 gaacaggcgt tgcgcgatgc gcgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt
    44461 ggcggcgcgt cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta
    44521 ccgtatcaaa gttacgatcc gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc
    44581 tgccgcttac gcagtgaaga tattgaagaa gtgatcctca ctgatatttg cccttactcg
    44641 ttgggcgttg aggttaaccg ccagggcgtt tccggcattt tctcgccgat tattgaacga
    44701 aacaccactg tgcccgtgtc gcgtgtagaa acttattcaa ccatgcaccc ggaacaggat
    44761 tcaattacgg ttaacgtcta tcagggagaa aaccacaaag ttaaaaacaa cattctggtg
    44821 gaatccttcg atgtgccgtt gaagaaaacc ggggcttatc agtcgattga tattcgcttt
    44881 agttatgata tcaacgggtt gcttgaagtt gacgtgcttc tggaagacgg cagcgttaag
    44941 tccagagtga ttaaccacag cccggtaaca ttgagcgcgc agcagattga agagagtcgg
    45001 acgcggttat ccgcattgaa aatttatccg cgcgatatgc tcatcaatcg cacctttaaa
    45061 gccaaactgg aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg
    45121 gtgatcaccg attttgatgc ggcgctgcag tcaaacgata tggcccgcgt cgatgaggtc
    45181 aggcggcggg cgagcgttta tttagccatt gagacctcat aatcacggct gccggatgcg
    45241 gcgtaaatgc tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg
    45301 attttgccga gagcataacc tataaccagc accgttaaac tgattttgat aatgctggta
    45361 atgctctgtg gtttttcacc ctcatcattt ttttcttcat ggttttcagg tgttggctca
    45421 tcgacatctt catcctccgg ctcgagtttt actttcgtca acagcatccg ggtgataatg
    45481 gcctctaatg gatgagcatc tccgccttcc agccttgtat taagacgggc cagcatctct
    45541 tgtttcggaa acgcttcaag tactacctgt tccgctaccc gatacaaatc cagtaacggt
    45601 gaatcttctt ctgtctcgct accgactctc gatttccacc acgcccatgc tttgcgtttg
    45661 aagaataacg actcaataaa cgattgacct aacggcgata attttccgct atgtaaaatt
    45721 tccgcccata acagccgcgt ccgggcgctc tcaccaggaa catatttcag cggttgacct
    45781 tgcgcatcta ccatgctccg cgcttcgata accatcacta ataacgcgaa ataatccggg
    45841 caatgacgct tgctccactg caacaacaaa ttatcagctt gtgtagatgg gtaactttcc
    45901 cagtacgcgc cgagatcagc aagcaggctt tccccctcgc cgcagtacaa acgttgtgca
    45961 cactggtaat accgggcatc ttcgctttct ggttcttccg cttgccactg tttggctaca
    46021 ggaatgagtt ccaccatacc tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt
    46081 aactttgcat catcaggaat gaaccacggt ccttccatcg ccaaaaacgc agtgacatag
    46141 tcaggatggt aacggcagca acgttccagc gcgaagtaat attcaacagt ctggttctgt
    46201 aacgcgacgg gatattccag aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc
    46261 tcaagaaaag cctccctttc ttcctcatcc attccttcgc tgtcattaaa cgaatgccag
    46321 ttcaaacgct ctgccaacaa agaagcacaa tttaatgaca gatatcgcgc ttctatagcg
    46381 attgcacaca gcggccagcg taattgatcg acatcctcca tgttccaggt atttaattgc
    46441 tgaataaatt tctgccaggc ggagggctga aaacgatcac tttctgaatc cagtaatgtc
    46501 ctgaatgcac gtagtatttc atgttcagcg gtggcatctt tttcttcatc atctgcttcc
    46561 tcgacaggat ttaccgccag tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac
    46621 ccctgcggat cggtttcggg atgacacaac ggtaagcgag caagataagc ctggcggata
    46681 atgtctattt gcgtcgtgct ttcaatttcg aggatttgcc aacatgtttt cataggtcgc
    46741 tcacatcctt aagcgttacc gggcattcag gctggacagt taatggctgc cagggaagcg
    46801 tggcatagaa acggtcgctt cccgctgcca gctcttcgaa catccgactc ccggcgctat
    46861 cgtatttgta cagggttagc gcctcgcgta gcgaagcgtt gtcttcgcac tgagaagatt
    46921 gccagtagag acgccctagt gaataggcag aaaaatagtg catccagctg tgatagtaat
    46981 ggtgagctcg tgcatagacg cgcgattgca gccataaact ctcttcttcc gtcagccagt
    47041 tgttgagcac accggcccgt gacagaaacc ccattcgaac ataatcccag gctttgattc
    47101 ccccttctcc acagcacata gcggtttggg caacgaattg ggtataaatc aaccctctct
    47161 ccgagccacc agcaattaat gctttccact cttcagggga agagcgttgc catgtcagat
    47221 ataagccagc taaatcattg gcatgaccat catcagtcat ctgataaata gtctgaatta
    47281 aatcatcacg attatttatt ccccaggatg atgagagatc gaattctttt atcccggtat
    47341 acatttttgg cgagtcatgc gcgggtttgc aactctgatt aagtatttcc atgggcgcag
    47401 aaagcgcaaa aagaagatat tgttcttcct tattcatttt ttaataatcc cttaagtgca
    47461 ttaactgact acttttttat tattgatgag tataaacttg acgctttcaa aataaaaaga
    47521 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt
    47581 gcagcaatta cctgtattag ccaacccaag aaaacgatgt tgcgtgataa agccatggtg
    47641 aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg
    47701 gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt
    47761 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
    47821 gcagaacagg aaaatccgcg cgcacaaagc aaactgaggt ggatctacct gaaaggcctg
    47881 ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
    47941 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt
    48001 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
    48061 gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag
    48121 tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag
    48181 acagcccagc ccgccatcat caacgggcat tggctgtggt tgcggctccg gctcgacggt
    48241 ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
    48301 tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg
    48361 ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt
    48421 atcacgcgtg agtgagatcc gcggcttacg tccttcgtgc aaaaccatct cgaccagtga
    48481 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
    48541 ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc
    48601 ttcaatcatc atcaccacta atgggtaata atagacccga tgctgacggc accattgcaa
    48661 cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa gttctgccag
    48721 caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc
    48781 attgtcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag
    48841 gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat
    48901 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
    48961 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaactggca gatgcagaat
    49021 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
    49081 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
    49141 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
    49201 ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
    49261 ggacagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
    49321 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
    49381 tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
    49441 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
    49501 tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag cgtttccggg
    49561 catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc atcatcactg
    49621 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
    49681 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
    49741 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc
    49801 gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt
    49861 aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct
    49921 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca
    49981 cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
    50041 tgaccgttct cgatcattcg gtgaattatg cgcagcaggt catcacggtc atcaattccc
    50101 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga tgaatgcgaa
    50161 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
    50221 gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc
    50281 gaggctcagt tatttatact tttctgacgc aacataatac gcataaaata aggcttacgc
    50341 aaaaatggaa tacataaaaa tataatatat tttatctaca accataagga aatgataatg
    50401 atttttacgt caagttgctg cgataattta tcaatagatg agattatcga acgtgctgaa
    50461 aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt
    50521 gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga gcaaggtcat
    50581 tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg tcataaagat
    50641 aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc
    50701 aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga agcggttacc
    50761 tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct tggatggatg
    50821 tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg gtacaaacaa
    50881 gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta taaagacgga
    50941 gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag cgcacagcag
    51001 ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga aggggtggac
    51061 caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga aagcgtcggc
    51121 gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag
    51181 gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc ctggtataac
    51241 ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac
    51301 ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat tcgtgagact
    51361 gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc catattctct
    51421 ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcata
    51481 accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca
    51541 tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa ccccatgatg
    51601 attgaggtcc tggaaaattt cgcgccattc aagctgcgtt ttatcggtga tgtctgccag
    51661 ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt cttctttcag
    51721 gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc gcgtcagctc
    51781 atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac
    51841 cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg cgaccagttc
    51901 acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa
    51961 taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt
    52021 acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg
    52081 tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt agccgtattg
    52141 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
    52201 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
    52261 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
    52321 cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg caaaaacgtt
    52381 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
    52441 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
    52501 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
    52561 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taaaaagact
    52621 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
    52681 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
    52741 aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca
    52801 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc
    52861 ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt
    52921 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
    52981 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct
    53041 gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt tcacccatta
    53101 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
    53161 gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc
    53221 ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct ttctcagcaa
    53281 gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct tgaccatgaa
    53341 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
    53401 aactaccgcc tgcgcgactg gggtgtttct cgccagcgtt actggggcgc gccgattccg
    53461 atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct gccggtgatc
    53521 ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg
    53581 gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt
    53641 atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg
    53701 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
    53761 gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
    53821 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
    53881 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
    53941 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
    54001 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
    54061 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
    54121 atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct gaaacgtgtc
    54181 tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg
    54241 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc taaagtgacc
    54301 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
    54361 aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat gcaggaagca
    54421 ctgcaggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt cacgctgtgg
    54481 caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
    54541 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa
    54601 atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
    54661 ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt accaggtaaa
    54721 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacactgt
    54781 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gacaccacgc
    54841 aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa
    54901 gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
    54961 cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat
    55021 cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg
    55081 tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt tcattcttcg
    55141 ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt
    55201 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
    55261 gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct
    55321 ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
    55381 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
    55441 ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc acactttttc
    55501 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt
    55561 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa
    55621 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctattga tcgtccgcgg
    55681 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag
    55741 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgtgcg
    55801 cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct gctactgtta
    55861 tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga
    55921 cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc
    55981 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca
    56041 gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac aacgtgaact
    56101 gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
    56161 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
    56221 tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
    56281 agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa
    56341 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
    56401 gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg gcgaatttga
    56461 ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
    56521 aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
    56581 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
    56641 aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt ggtcaggatt
    56701 cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
    56761 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
    56821 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
    56881 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
    56941 acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt aaccaacaag
    57001 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
    57061 tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac
    57121 cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg tttcctttga
    57181 cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga
    57241 ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg gcgactggga
    57301 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
    57361 tgactggcag catgctatcc ccacgaatgg tgaaggattt caggcatttt cgcaacgtgt
    57421 ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt taatcgtcag
    57481 ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg ccgagtctat
    57541 gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc aaaaattcgc
    57601 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aacgcatgac ttttctgttt
    57661 ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc
    57721 cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    57781 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    57841 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    57901 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    57961 aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga
    58021 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    58081 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    58141 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    58201 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
    58261 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    58321 tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg gacatcaagc
    58381 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    58441 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
    58501 ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    58561 cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
    58621 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    58681 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    58741 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
    58801 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    58861 ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg
    58921 cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc aaccgcacta
    58981 tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt
    59041 tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    59101 gttcacaccg tttcacctct attccggtga aaactaacct aaccgaagta caagtagctc
    59161 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    59221 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    59281 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    59341 ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    59401 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    59461 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    59521 tgctggcggg tagccgcgca gctgtggttg ttaccgaacc gcgtacaggt ggggtgctgg
    59581 cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    59641 attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg
    59701 tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta agcgccgggg
    59761 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca gggtcggaaa
    59821 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
    59881 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    59941 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    60001 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    60061 agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc ccaatccaga
    60121 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
    60181 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg
    60241 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    60301 gccctaatgg tacggcgaat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    60361 ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    60421 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    60481 tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc
    60541 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    60601 cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    60661 ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta
    60721 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    60781 cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    60841 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    60901 tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt
    60961 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    61021 cgacgtttgc ccgccatcgt tgaagaatac tggcatcgcg ctggtgctga tatttatgcc
    61081 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    61141 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    61201 ggtagcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    61261 cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca
    61321 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    61381 gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg tactggcgga
    61441 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    61501 cgggatgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    61561 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    61621 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    61681 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    61741 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    61801 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    61861 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    61921 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    61981 gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg
    62041 cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg cttccgatcc
    62101 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    62161 atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc
    62221 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    62281 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    62341 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    62401 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    62461 gtagcggttt cctcggcgct ccaacgacct tagcgcctag tgtactggaa ggcagcgaac
    62521 cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca acctcgcctg
    62581 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg
    62641 gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    62701 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg
    62761 ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    62821 catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    62881 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    62941 ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    63001 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    63061 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    63121 agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg
    63181 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    63241 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    63301 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    63361 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    63421 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    63481 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    63541 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    63601 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    63661 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    63721 acaaagcagg ttacaatctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg
    63781 cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    63841 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    63901 aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc
    63961 tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    64021 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    64081 gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    64141 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaaattg
    64201 aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa aactcccgcc
    64261 ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa accaaactta
    64321 acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag gcgttacctg
    64381 agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac tacaccccaa
    64441 cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc aacgccactc
    64501 atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg
    64561 ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct ctcctcgctg
    64621 tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt gtatcaggat
    64681 aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctcccg ggctatgcat
    64741 gaattcaccg atacccgcga taatagtacc cttgatgaaa tctggctggt cgagcactat
    64801 ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt
    64861 ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg acaacaggtg
    64921 atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg
    64981 cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca tcctcgggct
    65041 gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt acgaattcga
    65101 cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc accattttta
    65161 cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca atggaaaccc
    65221 gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc gctactaaac
    65281 aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca atttacactg
    65341 ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt atattttctt
    65401 tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat tgattaacct
    65461 attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca taagattatt
    65521 gtaatttttt aaatattatt ttcccatgaa taaaattgga gtaagtgcgt ggatagtaat
    65581 aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca aatatttaca
    65641 accttacgaa atattgatct taaccttctg actatttttg aagctgtata tgtacataaa
    65701 gggatcgtta atgcagcgaa agtgcttaat ctgaccccct cggcaatcag tcagtctatt
    65761 cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca gggtgtcact
    65821 cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga gtccattctt
    65881 ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat tgcgaccact
    65941 ccctcggtcg gagccctggt ccttcctgtc atctatcggg cgattaaaac tcactatccg
    66001 cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag tcagtttcaa
    66061 accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca tcatgttctg
    66121 ttcaccgaca atatggtgtt aatttgccgc aagggaaatc cactactctc tttagaagat
    66181 gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaagg gcaaaatttc
    66241 agcggtctgc ggcagagagt tcaagagatg tttccggacc ggcaaatcaa tttcaccagc
    66301 tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc gattattccc
    66361 agccgttttt ataacctgtt tagtcgttgc tggccgctgg aaaaactgcc ttttccgtcc
    66421 ttaaatgagg agcaaataga tttctctatc cactacaaca aattcatcct gcgtgatccc
    66481 atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat tgttacagcc
    66541 agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca ttttcgcgac
    66601 cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata
    66661 gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa
    66721 cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga
    66781 taagatggcc cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa
    66841 gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg gcatcaaagc
    66901 cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc
    66961 ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt gtacccgccg
    67021 ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt
    67081 gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga
    67141 ccgtgatgac ctgcgcgatg gcggtgccca acactttgcg gattgtatta ctgccattcg
    67201 ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgcg gtcgaatgga
    67261 tcgtgctctg gatattctga ctgcaacgcc cccagatgtg ttcaaccata acctggaaaa
    67321 cgtaccgcgt atttaccggc aggtacggcc tggcgcagat tacaactggt cgctgaagct
    67381 gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc tgatggtggg
    67441 tctgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt
    67501 gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg
    67561 ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac
    67621 ccatgctgca tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg
    67681 aatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa aacccgcttt
    67741 ttgaagcggg ttttttgtat cgaacaagat attgagggag cgtcctgctc gccacgtcac
    67801 tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt cgccgcagca
    67861 tcgtcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc tccgcccagc
    67921 gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac cagcagtttg
    67981 gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca gaacgcgcct
    68041 taaaaaccag agaatattaa cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa
    68101 ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca aagcgacggt
    68161 tgtttaagat gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca
    68221 taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga tctataatcc
    68281 ggctttgccc gatatttttg ttcccgcact cccccgccgc caccatataa caggtggtat
    68341 ccagcgcacg agcggcaagc aacgttgacc agtgatgctc tttgagcggc ccacgaaccc
    68401 aggcggcagg aagtaccagg atttcagctc cctgtaaagc ctgtgccagc gccagctctg
    68461 gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccttccacc tccagtaacg
    68521 gagctatttc attaccagca tcaacatggc gtgattcctg aatggcaaat gcatcataga
    68581 gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc agcatattcc
    68641 atgcgcgccc cggcgttgaa ggaagatgaa tcgtcagaat tgtcgtcatc atgttacgtt
    68701 tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct gatttaaccg
    68761 atagatctgc atcatgatca tcgcgcgcca gcaatgcttc cggcaggaca aacagcgata
    68821 cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc ttttcccaca
    68881 cagatgtaac agcaaactgt cctgcagcaa ccagcattgc cacctcctgt cagagtcgta
    68941 aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat caaatagcag
    69001 gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac gggaagcgta
    69061 gcgagatggc tgttaagtat gcgatttaac ccgctgcatc aggcgattcc gttggggacg
    69121 ctggcagcaa acctgattgg ggcattcatc ataggaatgg gattcgcctg gttcagcagg
    69181 atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg tggcggtcta
    69241 acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc
    69301 tgggcattac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac cgcactggca
    69361 ttctggctgt tttcggcctc aaccgtacac taaagaccaa aaaaaacccg ctgattaagc
    69421 gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc agaagggcct
    69481 ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt tttaaaacca
    69541 ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg
    69601 aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt agacatcaaa
    69661 attaccttta catgaaaaat agacacaaat gctgtgtcgg ttatcagtac accaattgtg
    69721 gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta tttttgatgt
    69781 tgcttatttt agttattgat tttaaagaag ttactaaaac ttcatttgtc tcaaaactga
    69841 aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat gtaggtcatc
    69901 tgaaaagtca ctgggccata acccacggag gccaatggca gtagaaccgg gagagggatg
    69961 taattccagt tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa atgaaaattt
    70021 tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg ttcccatttg
    70081 ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt ttcatcccaa
    70141 cgcgacaggc caaaaccgcc accccacggt cgctcgttat agcgatcggt tttttctttg
    70201 tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat ataaatcata atgttcaggc
    70261 tgttgccagg tttgtgcaat attttctcta aacgttgtca tcctctcatc tgcgttagca
    70321 aaaactttac caacgctgat taactgaata aaaacaaagg aaaagatagc gacatattta
    70381 ctcacgttca tttgtgacca taaaacattt atcaaaaatc tactactagc atagcaaagc
    70441 tacaattaac ataaccttaa tagacccaac ataaagaata atctgaatag ctgctctatt
    70501 tatttccgct tgttcaagtt aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt
    70561 ctgcgatggg aatagtcaaa aaagaaaaac caagtctttt ttgatgacaa aatgcaatca
    70621 aggaaaaatt attttatttt tttaacgaga tacaacaatc atcttaacga agtatataat
    70681 atatatcgtc aaccatgctc atattagatt attttttgag cgcacagccg cagctaacac
    70741 cattgcaatt aacaaatttg catcaatcca ccatcaattt acacacatta ttatgtgata
    70801 attgccaacc gctaaatatg cgttttgtta tctatgtata aaaacagcaa cttcaatgtc
    70861 ttaatggcag tttttcttga ttttaatcag cattcatcgc caatttattg ggcatatttt
    70921 ttccttaagc tttaggaagt ttttattatt tactttgggc ctggagacag gaaaaattat
    70981 gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtgg ctcgctacat
    71041 cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat tgctgattat
    71101 cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc
    71161 cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg acctcggcat
    71221 gatgattatg atgctgtgtg gctttgccgc ttacatgacc catatcggcg cgaatgatat
    71281 ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc tgctgatgat
    71341 tgccgcctat tttgttgctt gtctgatgtc actggccgtc tcttccgcaa ccggtctggg
    71401 tgttttgctg atggcaaccc tgtttccggt gatggtaaac gttggtatca gtcgtggcgc
    71461 agcagctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt
    71521 ggtgctggcg gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct
    71581 gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta
    71641 tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa tcaccaccac
    71701 tgcccccgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac tgatttttga
    71761 cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc
    71821 ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt
    71881 ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg
    71941 ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac
    72001 ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga ccatgctggc
    72061 ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa
    72121 actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc
    72181 aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg ccgggatggc
    72241 gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg ttgggctagt
    72301 gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacagacaa
    72361 gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat
    72421 atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg
    72481 gcgcaatatt caagataacg cctggcagtg gtgcggctga tggtcagtgc ctgcgccacc
    72541 gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc
    72601 aaggggtcaa tgccggtcgg cagttcgtct ttgggttcac cgcgagcata ggcattaaac
    72661 atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg
    72721 cggaaacggg ttagcgtttg ccccagtgta ttgattcact tgaagtacga aaaaaccggg
    72781 agaacattgg attattcggg atctgatggg attagatttg gtggggcttg caagcctgta
    72841 gtacaaattt taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt gcttttgttt
    72901 cattgaaaaa acgaaaaaca aaaacactat aaatcatttc aatcatagct tcaaaaaacg
    72961 ttcaaaaccg ataacaacca ggctgtaacc aaatgactca tatcacaaat cagctcatgc
    73021 cgtttaggta tgttacatgt gtgattatgt gaggtgaagt atgttttagc tggctcatgg
    73081 ttgttatacg gcttttttcg catcttgtgg ttccaacaaa ggagctacag gctcttcctg
    73141 tttatgaaca ccatacattg acaaaatctc ttggcgagtt tctggaggta atttatgcag
    73201 cgccaaagct gtttgtagtg tttggcgatc aatacccagc gttgccaata gggtgtcaac
    73261 ggagatggag gggcgttctt gcattaatcc ggaaagccca tatttagcca tgtttagctc
    73321 tatctctcgg acttcatttt ctcctagttt attgattaac agggagaaca ttttcaatgt
    73381 atcagcattc atttcttcga tagcaatttt ttgcatagcc ggtattgccg ggatatattc
    73441 aaccccaggc ccgcgacgcc cttcttgtga acgcttcggc catccttctt tctttgctcg
    73501 tttatgcacc ccctgtattg tcccaggcat tccatccagt tcagtaattt gtttgggtgt
    73561 catccactgg tttatctcat ttgcgctttt cataaactaa gtttaccaga tttatctata
    73621 taaaaatcaa tggattacaa aataaaccac attaactgat ataaaccggt tttgtttatt
    73681 tggtaatgta taaaacagat aatcaagacg aattatttgt tttggtaacc ctttatcata
    73741 cgcaatatgg ttggtgtgcg aaagatgagt aacgattggc atcgggccga cgtgattgcg
    73801 gccctcaaaa aaacaggaac ccctctggca aaattgtcca gacgttcagg ttattcaacc
    73861 gggacgcttg caaatgcatt agtcaggcca tggcctaaag gtgaacgcat tattgctgac
    73921 gccttaaaca tgaaacctga ggaaatttgg cctagtagat atctaaggga tcacaatgag
    73981 agagtggata tcagcaattg aatgcatggg tttatctggt ttacctggtt cattaatggg
    74041 ggttcacaaa agagctcaga gagaaggatg gctcaagaga agaagagaag gaattagagg
    74101 ggttgcttac gaataccata tatcttctct tcctaacatt gctcgagctg aagttttgct
    74161 ccaacaagga aaatttaaaa ccagtcaggg atgttttgaa atcgcccgcc ccacgctgga
    74221 agctcatgat tatgatcgtg aggcactatg gagcaaatgg gataaagcca gcgattccca
    74281 gcgcagcctg gctgaaaaat ggttgccctc aattcaggcc acagacgaaa tgctgaacca
    74341 ggggatttca acgaaaacgg cttttgcgac cgttgcaggg cattaccagg tcagcgcatc
    74401 cactttgcgg gacaagtatt accaggtaca gaagtttgcg aagcctgact gggcggctgc
    74461 acttgttgat ggacgtggag catcccgtcg caatgttcac aaaagtgaat ttgacgagga
    74521 tgcctggcag tttctgattg cagattatct gcgaccggaa aaacccgcct tccgcaaatg
    74581 ttatgagcgt ctggaactgg cagcccgcga gcatggctgg agtattccct cccgtgccac
    74641 ggcctttcgc cggattcagc aactggacga ggcaatggtt gttgcctgtc gtgaaggtga
    74701 acatgcactg atgcatctga taccggcaca gcagcgaact gtggaacacc tggacgccat
    74761 gcagtggatc aacggcgacg gttatctgca taacgtcttt gtacgctggt ttaacggtga
    74821 tgtgatccgt ccgaaaacat ggttctggca ggatgtgaaa acccgaaaaa ttctgggctg
    74881 gcgctgcgat gtgagcgaga acattgattc aattcgcctc tcgttcatgg atgttgtgac
    74941 tcgctacagt atcccggagg attttcacat caccattgat aacacccgtg gtgctgcgaa
    75001 taaatggctg acgggaggcg cgcccaatcg ctaccgcttt aaggtaaaag aggacgatcc
    75061 aaaaggactg tttttactga tgggtgcgaa aatgcactgg acaagcgttg ttgccggtaa
    75121 aggctggggc caggcaaaac ctgttgaacg tgctttcggt gttggtgggc ttgaggaata
    75181 cgttgataag catccggcac tggctggcgc atatacgggg ccaaatccgc aggcaaaacc
    75241 tgataactat ggcgaccacg ctgttgatgc agagctgttt ctgaaaacct ttgccgaagg
    75301 tgtggcgatg ttcaatgcca gaacaggccg tgaaacagaa atgtgcgggg gcaaactctc
    75361 gtttgatgat gttttcgagc gtgaatacgc cagaacgatt gtgcgtaagc caaccgaaga
    75421 acaaaaacgg atgctgttac tgcctgccga ggcggtgaac gtttcacgca aaggcgagtt
    75481 tacgcttaaa gttggcggct cccttaaagg cgcgaaaaac gtttattaca acatggcatt
    75541 aatgaatgcc ggagtgaaaa aagttgtggt cagatttgat ccacagcagt tacacagcac
    75601 ggtttattga g
//
LOCUS       DAEEDS010000005        72215 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEEDS010000005 DAEEDS010000000
VERSION     DAEEDS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72215
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.005"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            28..1149
                     /gene="roxA"
                     /locus_tag="KLH32_000345"
                     /gene_synonym="ycfD"
     CDS             28..1149
                     /gene="roxA"
                     /locus_tag="KLH32_000345"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBE0137324.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(1198..2424)
                     /gene="pepT"
                     /locus_tag="KLH32_000346"
     CDS             complement(1198..2424)
                     /gene="pepT"
                     /locus_tag="KLH32_000346"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBE0137325.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            2674..3810
                     /gene="potA"
                     /locus_tag="KLH32_000347"
     CDS             2674..3810
                     /gene="potA"
                     /locus_tag="KLH32_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBE0137326.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            3794..4651
                     /gene="potB"
                     /locus_tag="KLH32_000348"
     CDS             3794..4651
                     /gene="potB"
                     /locus_tag="KLH32_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBE0137327.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            4648..5442
                     /gene="potC"
                     /locus_tag="KLH32_000349"
     CDS             4648..5442
                     /gene="potC"
                     /locus_tag="KLH32_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBE0137328.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            5439..6485
                     /gene="potD"
                     /locus_tag="KLH32_000350"
     CDS             5439..6485
                     /gene="potD"
                     /locus_tag="KLH32_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBE0137329.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(6641..7462)
                     /gene="cobB"
                     /locus_tag="KLH32_000351"
     CDS             complement(6641..7462)
                     /gene="cobB"
                     /locus_tag="KLH32_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBE0137330.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(7478..8389)
                     /gene="nagK"
                     /locus_tag="KLH32_000352"
     CDS             complement(7478..8389)
                     /gene="nagK"
                     /locus_tag="KLH32_000352"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBE0137331.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8418..9662)
                     /gene="lolE"
                     /locus_tag="KLH32_000353"
     CDS             complement(8418..9662)
                     /gene="lolE"
                     /locus_tag="KLH32_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBE0137332.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9662..10363)
                     /gene="lolD"
                     /locus_tag="KLH32_000354"
     CDS             complement(9662..10363)
                     /gene="lolD"
                     /locus_tag="KLH32_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBE0137333.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10356..11555)
                     /gene="lolC"
                     /locus_tag="KLH32_000355"
     CDS             complement(10356..11555)
                     /gene="lolC"
                     /locus_tag="KLH32_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBE0137334.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11817..12890
                     /locus_tag="KLH32_000356"
     CDS             11817..12890
                     /locus_tag="KLH32_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBE0137335.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            13018..16464
                     /gene="mfd"
                     /locus_tag="KLH32_000357"
     CDS             13018..16464
                     /gene="mfd"
                     /locus_tag="KLH32_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBE0137336.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16607..17569
                     /gene="ldtC"
                     /locus_tag="KLH32_000358"
     CDS             16607..17569
                     /gene="ldtC"
                     /locus_tag="KLH32_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBE0137337.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17652..17864)
                     /locus_tag="KLH32_000359"
     CDS             complement(17652..17864)
                     /locus_tag="KLH32_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0137338.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            18149..18781
                     /gene="comR"
                     /locus_tag="KLH32_000360"
     CDS             18149..18781
                     /gene="comR"
                     /locus_tag="KLH32_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBE0137339.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(18843..19382)
                     /locus_tag="KLH32_000361"
     CDS             complement(18843..19382)
                     /locus_tag="KLH32_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE0137340.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(19609..20913)
                     /gene="ndh"
                     /locus_tag="KLH32_000362"
     CDS             complement(19609..20913)
                     /gene="ndh"
                     /locus_tag="KLH32_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBE0137341.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(21321..21863)
                     /locus_tag="KLH32_000363"
     CDS             complement(21321..21863)
                     /locus_tag="KLH32_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0137342.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(21886..22911)
                     /gene="nagZ"
                     /locus_tag="KLH32_000364"
     CDS             complement(21886..22911)
                     /gene="nagZ"
                     /locus_tag="KLH32_000364"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBE0137343.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(22922..23746)
                     /gene="thiK"
                     /locus_tag="KLH32_000365"
     CDS             complement(22922..23746)
                     /gene="thiK"
                     /locus_tag="KLH32_000365"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBE0137344.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(23727..24368)
                     /gene="lpoB"
                     /locus_tag="KLH32_000366"
     CDS             complement(23727..24368)
                     /gene="lpoB"
                     /locus_tag="KLH32_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBE0137345.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(24383..24760)
                     /locus_tag="KLH32_000367"
     CDS             complement(24383..24760)
                     /locus_tag="KLH32_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBE0137346.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(24763..25122)
                     /gene="hinT"
                     /locus_tag="KLH32_000368"
     CDS             complement(24763..25122)
                     /gene="hinT"
                     /locus_tag="KLH32_000368"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBE0137347.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            25253..25434
                     /locus_tag="KLH32_000369"
                     /pseudo
     CDS             25253..25434
                     /locus_tag="KLH32_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25467..27652
                     /gene="fhuE"
                     /locus_tag="KLH32_000370"
                     /pseudo
     CDS             25467..27652
                     /gene="fhuE"
                     /locus_tag="KLH32_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(27712..29145)
                     /gene="ptsG"
                     /locus_tag="KLH32_000371"
     CDS             complement(27712..29145)
                     /gene="ptsG"
                     /locus_tag="KLH32_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBE0137348.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(29440..30237)
                     /locus_tag="KLH32_000372"
     CDS             complement(29440..30237)
                     /locus_tag="KLH32_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0137349.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(30248..31252)
                     /gene="holB"
                     /locus_tag="KLH32_000373"
     CDS             complement(30248..31252)
                     /gene="holB"
                     /locus_tag="KLH32_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBE0137350.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(31249..31890)
                     /locus_tag="KLH32_000374"
     CDS             complement(31249..31890)
                     /locus_tag="KLH32_000374"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBE0137351.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(31880..32902)
                     /gene="yceG"
                     /locus_tag="KLH32_000375"
     CDS             complement(31880..32902)
                     /gene="yceG"
                     /locus_tag="KLH32_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBE0137352.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(32905..33714)
                     /gene="pabC"
                     /locus_tag="KLH32_000376"
     CDS             complement(32905..33714)
                     /gene="pabC"
                     /locus_tag="KLH32_000376"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBE0137353.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(33834..35075)
                     /gene="fabF"
                     /locus_tag="KLH32_000377"
     CDS             complement(33834..35075)
                     /gene="fabF"
                     /locus_tag="KLH32_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBE0137354.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(35162..35398)
                     /gene="acpP"
                     /locus_tag="KLH32_000378"
     CDS             complement(35162..35398)
                     /gene="acpP"
                     /locus_tag="KLH32_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE0137355.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(35609..36343)
                     /gene="fabG"
                     /locus_tag="KLH32_000379"
     CDS             complement(35609..36343)
                     /gene="fabG"
                     /locus_tag="KLH32_000379"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBE0137356.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(36356..37285)
                     /gene="fabD"
                     /locus_tag="KLH32_000380"
     CDS             complement(36356..37285)
                     /gene="fabD"
                     /locus_tag="KLH32_000380"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBE0137357.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(37301..38254)
                     /gene="fabH"
                     /locus_tag="KLH32_000381"
     CDS             complement(37301..38254)
                     /gene="fabH"
                     /locus_tag="KLH32_000381"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBE0137358.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(38322..39392)
                     /gene="plsX"
                     /locus_tag="KLH32_000382"
     CDS             complement(38322..39392)
                     /gene="plsX"
                     /locus_tag="KLH32_000382"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBE0137359.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(39473..39646)
                     /gene="rpmF"
                     /locus_tag="KLH32_000383"
     CDS             complement(39473..39646)
                     /gene="rpmF"
                     /locus_tag="KLH32_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBE0137360.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(39698..40219)
                     /gene="yceD"
                     /locus_tag="KLH32_000384"
     CDS             complement(39698..40219)
                     /gene="yceD"
                     /locus_tag="KLH32_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBE0137361.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            40418..41002
                     /gene="yceF"
                     /locus_tag="KLH32_000385"
     CDS             40418..41002
                     /gene="yceF"
                     /locus_tag="KLH32_000385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBE0137362.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(41114..42073)
                     /gene="rluC"
                     /locus_tag="KLH32_000386"
     CDS             complement(41114..42073)
                     /gene="rluC"
                     /locus_tag="KLH32_000386"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBE0137363.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            42646..45831
                     /gene="rne"
                     /locus_tag="KLH32_000387"
     CDS             42646..45831
                     /gene="rne"
                     /locus_tag="KLH32_000387"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBE0137364.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(46027..46980)
                     /gene="flgL"
                     /locus_tag="KLH32_000388"
     CDS             complement(46027..46980)
                     /gene="flgL"
                     /locus_tag="KLH32_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBE0137365.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(46992..48634)
                     /gene="flgK"
                     /locus_tag="KLH32_000389"
                     /pseudo
     CDS             complement(46992..48634)
                     /gene="flgK"
                     /locus_tag="KLH32_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(48700..49641)
                     /gene="flgJ"
                     /locus_tag="KLH32_000390"
     CDS             complement(48700..49641)
                     /gene="flgJ"
                     /locus_tag="KLH32_000390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBE0137366.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(49641..50738)
                     /gene="flgI"
                     /locus_tag="KLH32_000391"
     CDS             complement(49641..50738)
                     /gene="flgI"
                     /locus_tag="KLH32_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBE0137367.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(50750..51448)
                     /gene="flgH"
                     /locus_tag="KLH32_000392"
     CDS             complement(50750..51448)
                     /gene="flgH"
                     /locus_tag="KLH32_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBE0137368.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(51501..52283)
                     /gene="flgG"
                     /locus_tag="KLH32_000393"
     CDS             complement(51501..52283)
                     /gene="flgG"
                     /locus_tag="KLH32_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBE0137369.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(52421..53172)
                     /gene="flgF"
                     /locus_tag="KLH32_000394"
                     /pseudo
     CDS             complement(52421..53172)
                     /gene="flgF"
                     /locus_tag="KLH32_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(53192..54400)
                     /gene="flgE"
                     /locus_tag="KLH32_000395"
     CDS             complement(53192..54400)
                     /gene="flgE"
                     /locus_tag="KLH32_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBE0137370.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(54425..55120)
                     /gene="flgD"
                     /locus_tag="KLH32_000396"
     CDS             complement(54425..55120)
                     /gene="flgD"
                     /locus_tag="KLH32_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBE0137371.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(55132..55535)
                     /gene="flgC"
                     /locus_tag="KLH32_000397"
                     /pseudo
     CDS             complement(55132..55535)
                     /gene="flgC"
                     /locus_tag="KLH32_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(55539..55955)
                     /gene="flgB"
                     /locus_tag="KLH32_000398"
     CDS             complement(55539..55955)
                     /gene="flgB"
                     /locus_tag="KLH32_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBE0137372.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            56110..56769
                     /gene="flgA"
                     /locus_tag="KLH32_000399"
     CDS             56110..56769
                     /gene="flgA"
                     /locus_tag="KLH32_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBE0137373.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            56845..57138
                     /gene="flgM"
                     /locus_tag="KLH32_000400"
     CDS             56845..57138
                     /gene="flgM"
                     /locus_tag="KLH32_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBE0137374.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            57143..57559
                     /gene="flgN"
                     /locus_tag="KLH32_000401"
     CDS             57143..57559
                     /gene="flgN"
                     /locus_tag="KLH32_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBE0137375.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(57599..59134)
                     /gene="murJ"
                     /locus_tag="KLH32_000402"
     CDS             complement(57599..59134)
                     /gene="murJ"
                     /locus_tag="KLH32_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBE0137376.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(59301..60167)
                     /locus_tag="KLH32_000403"
     CDS             complement(59301..60167)
                     /locus_tag="KLH32_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0137377.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(60169..60816)
                     /locus_tag="KLH32_000404"
                     /pseudo
     CDS             complement(60169..60816)
                     /locus_tag="KLH32_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(60827..61411)
                     /gene="rimJ"
                     /locus_tag="KLH32_000405"
     CDS             complement(60827..61411)
                     /gene="rimJ"
                     /locus_tag="KLH32_000405"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBE0137378.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            61647..62855
                     /gene="mdtH"
                     /locus_tag="KLH32_000406"
     CDS             61647..62855
                     /gene="mdtH"
                     /locus_tag="KLH32_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBE0137379.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            62919..63566
                     /gene="grxB"
                     /locus_tag="KLH32_000407"
     CDS             62919..63566
                     /gene="grxB"
                     /locus_tag="KLH32_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBE0137380.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            63700..64260
                     /locus_tag="KLH32_000408"
     CDS             63700..64260
                     /locus_tag="KLH32_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0137381.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            64366..65412
                     /gene="pyrC"
                     /locus_tag="KLH32_000409"
     CDS             64366..65412
                     /gene="pyrC"
                     /locus_tag="KLH32_000409"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBE0137382.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            65486..65731
                     /gene="dinI"
                     /locus_tag="KLH32_000410"
     CDS             65486..65731
                     /gene="dinI"
                     /locus_tag="KLH32_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBE0137383.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            66021..66275
                     /gene="bssS"
                     /locus_tag="KLH32_000411"
     CDS             66021..66275
                     /gene="bssS"
                     /locus_tag="KLH32_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBE0137384.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            66390..67508
                     /gene="solA"
                     /locus_tag="KLH32_000412"
     CDS             66390..67508
                     /gene="solA"
                     /locus_tag="KLH32_000412"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBE0137385.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            67556..67669
                     /locus_tag="KLH32_000413"
     CDS             67556..67669
                     /locus_tag="KLH32_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBE0137386.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            67930..68496
                     /locus_tag="KLH32_000414"
     CDS             67930..68496
                     /locus_tag="KLH32_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBE0137387.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            68500..69075
                     /locus_tag="KLH32_000415"
     CDS             68500..69075
                     /locus_tag="KLH32_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBE0137388.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(69117..70169)
                     /locus_tag="KLH32_000416"
     CDS             complement(69117..70169)
                     /locus_tag="KLH32_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBE0137389.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            70394..71314
                     /gene="lpxL"
                     /locus_tag="KLH32_000417"
     CDS             70394..71314
                     /gene="lpxL"
                     /locus_tag="KLH32_000417"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBE0137390.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            71486..71683
                     /locus_tag="KLH32_000418"
     CDS             71486..71683
                     /locus_tag="KLH32_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBE0137391.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGADFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            71707..>72215
                     /locus_tag="KLH32_000419"
     CDS             71707..>72215
                     /locus_tag="KLH32_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0137392.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPGFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVS"
BASE COUNT        17574 a        19181 c        17938 g        17522 t
ORIGIN      
        1 gcagatacca gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat
       61 tttcttgaac gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt
      121 attgacccga tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt
      181 cgactggtca gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac
      241 gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg cagtaaacca ctggcatgag
      301 ccgaccgccg cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg
      361 atgatttctt tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg
      421 tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa
      481 cagcactgcc cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa
      541 gagctggagc ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg
      601 ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt
      661 agcggatttg ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatccg
      721 gatgttccac ctcgtgctca tcctgcggat gttctgccgc aagagatgga taaactgcgt
      781 gagatgatgc tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt
      841 atatcccagt cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat
      901 gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta
      961 ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg
     1021 gcactggatg cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg
     1081 gaagatccgt cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc
     1141 gaagggtaag tttggattta agccggatgc agcatcacat gccgcatccc ttttcgctta
     1201 cttccgttgc gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc
     1261 ttccagaatc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
     1321 gcaaggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
     1381 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
     1441 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccaatt caatgtagca
     1501 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
     1561 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt
     1621 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
     1681 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
     1741 tttcgccgtg cccggatgaa cattgttacc gacaattttt atattgaccg atgcggcgtt
     1801 gaagttttca aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc
     1861 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
     1921 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
     1981 gatttctgca atacctgctt tgtcatcagc acctaacaag gttttaccat cggtggtaat
     2041 caacgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
     2101 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt
     2161 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
     2221 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
     2281 gataagcccc atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
     2341 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
     2401 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
     2461 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
     2521 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
     2581 caagcggttt gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa
     2641 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
     2701 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
     2761 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
     2821 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
     2881 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
     2941 tatgtgaaca ctgttttcca aagctatgca cttttccccc acatgaccgt gttcgaaaat
     3001 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
     3061 gaagccctgc ggatggtgca gctggaaacc ttcgctcaac gtaaaccgca tcagctctct
     3121 ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg
     3181 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
     3241 ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt tcgtgactca cgaccaggaa
     3301 gaagcactca ccatgtcgga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
     3361 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg gttcattggc
     3421 gaaattaata tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc
     3481 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
     3541 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
     3601 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
     3661 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgat
     3721 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
     3781 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
     3841 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
     3901 gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct
     3961 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
     4021 cctcgcctgc ctggtgctgg gctatccatt tgcctggttt ctggcgaagc tgccacacaa
     4081 ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc tggaccaact cattgattcg
     4141 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
     4201 gctgggcgtt atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg
     4261 tctggtttac attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact
     4321 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
     4381 ccgcatcatt attccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct
     4441 gccagcgatg ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat
     4501 cggtaacgtc atcaaggtac agttcctcaa tattcgtgac tggccgtttg gtgcagccac
     4561 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
     4621 tttgctgaat aagaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
     4681 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
     4741 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
     4801 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
     4861 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc
     4921 ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga tgtcaccaga tatcgtgatg
     4981 gcaatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
     5041 ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg
     5101 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag cgaatttacc
     5161 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcggg ctgggtgtta
     5221 agctttaccc tgtcgatgga cgatgtggta gtttcttcat tcgtcaccgg accaagttat
     5281 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
     5341 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
     5401 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
     5461 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
     5521 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
     5581 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
     5641 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
     5701 ataaaatgcg taaagagggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
     5761 gcaatctcga tccagacatg ctcaacaagc cttttgaccc gaataacgac tactccattc
     5821 cgtatatctg gggtgcgacg gcgattggtg ttaacggtga tgcggtggat ccgaaatctg
     5881 tcaccagctg ggccgatctg tggaagccag agtacaaagg cagcctgctg ttgaccgacg
     5941 atgcccgtga agtgttccag atggcgctgc gtaagctggg ctactccggt aacaccaccg
     6001 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
     6061 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
     6121 tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac gtggtgtggc
     6181 cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
     6241 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
     6301 ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agtccagaag
     6361 tggcaaacga taagacgctc tacccggatg ctgaaaccat taaaaatggc gaatggcaga
     6421 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
     6481 gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
     6541 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccgcttgt
     6601 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc tccagcaact
     6661 tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
     6721 taccaacctg actcggttca agattcagtt caacggtgtg agcaccatgt agtttggctt
     6781 cgtgtacaaa cccagccgcc ggatagacat gcccggaagt accaatggca ataaagacat
     6841 ccgccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaaa
     6901 cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct tctggcgtaa
     6961 cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc agcaattccc
     7021 catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
     7081 ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt agccagcgca agatgcgcgg
     7141 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
     7201 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
     7261 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
     7321 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
     7381 aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt tttacgaaaa cgacttaacc
     7441 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
     7501 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag
     7561 ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg cgttgtgatt gccgggaaat
     7621 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
     7681 gagaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
     7741 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgataca
     7801 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
     7861 agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc agacgcatat
     7921 ggccaaactc gccggtaatg tagcttttac cagtaatcgg tttgccgttg aaaaccagcc
     7981 cgccgccaac gccggtgccg agaattaacc ccatcaccag tggatattgc gtaaattcat
     8041 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
     8101 caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct ggcagcgggg acattagcgg
     8161 catacagctt accatcttct gtttccggca ttcccggaat accgatgccg acagaacctt
     8221 tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct aaaaatgcgt
     8281 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
     8341 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
     8401 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
     8461 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
     8521 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaataaat atcgctggag
     8581 aggaactgat gaccgatcag cttttcaatc cactcaataa tcggggtaag ttgcagtgaa
     8641 acaactacgc cgataaccac cccacacagg ctgccgaaca gccctgccag caatccatac
     8701 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
     8761 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
     8821 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
     8881 atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt cacttcaccc
     8941 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
     9001 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
     9061 tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc
     9121 tgcatcagtt tatgctcggg attcgagttg gggatcatga tcgacaccca atcgccctgc
     9181 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
     9241 aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
     9301 tgtgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc caccagaccg
     9361 gtgaaattga tatacggcgc agcggcggca atacccggca ctttctgcac gttatccagt
     9421 gcttcctgcc agttagtcca tggctgattt accgcttcga tttcgccatg cggcaccacc
     9481 gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
     9541 aataccgcca cgccgagggc aatgccaatg gtagaaatga cagagattag cgacaccatg
     9601 ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca ataacgataa aggcatcgcc
     9661 attactccgc ccccatcagg ctcagttccg ccgtcagacg tccatcacgc atttccagtt
     9721 ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac caccaggaaa gcggtgccct
     9781 gcaagcgatt caactcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
     9841 taccggtagg ttcatccgcc agtaccagac gcggattatt gaccagcgca cgggcaatcg
     9901 ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
     9961 gccccaccgc ttttaacatc tccagtgcac agctgttgat ttcagcgggc tttttcttgc
    10021 caatcagcag tggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    10081 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgct gaagacagtt
    10141 tgctcatcgg ctgaccgtta aagatcacat cgccggaggt tggcgtatcc agcccgccta
    10201 acaggtgcag caaggtactt ttaccggaac cagagctacc gacaattgcc atcatttcac
    10261 cttcgcccac gctgaaactg acattgagca gcacatcggt ttgcacactg ccttcctgat
    10321 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    10381 ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    10441 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
    10501 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    10561 agcgccgctc cgaggattgc accgataatc ccggcgctag ccccttgcac cataaagacc
    10621 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgcgacttc gccctgcttc
    10681 tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacagc gacaatcagg
    10741 ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    10801 cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    10861 actctcagcg gctcatccag ccacaaacgc cagccggtaa tattggctgc cggataacgc
    10921 atcagacgcg atgcatcctc aatattcacc agcatttcat agccatcgac ttcactgttg
    10981 gcggcgaaag tgccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
    11041 aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattgac gcccaactgt
    11101 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    11161 acattgacca gatacggcgt tagtggatct ttttgcgccg ggtcgatacc gagcatcacc
    11221 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    11281 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    11341 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    11401 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    11461 atggtagaaa gccaggagac gaaacgaccg aagcgatcca ctgcacgccc acgcatgtaa
    11521 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    11581 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    11641 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat
    11701 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    11761 gggtcataac cagcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gttctgatga
    11821 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    11881 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    11941 tcctgagcaa atgctggatc tatttcaatc tttaccttgc cccctttcgt atgccggttt
    12001 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    12061 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    12121 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    12181 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    12241 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    12301 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    12361 ccacgccgtg gtggggaatg aacagtgtga tacgcaattt gccttattac agccttggcg
    12421 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    12481 tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
    12541 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat
    12601 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
    12661 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
    12721 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
    12781 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
    12841 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc
    12901 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    12961 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    13021 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    13081 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    13141 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    13201 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    13261 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    13321 cagcgtggca tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    13381 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    13441 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    13501 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    13561 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    13621 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    13681 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    13741 gaacatattt accagcaagt gagtaaaggc acattacctg ctgggatcga gtactggcag
    13801 ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg
    13861 ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaagctga cacgctggcg
    13921 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    13981 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact
    14041 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    14101 gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    14161 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    14221 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    14281 gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggc gcgtaatctg
    14341 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    14401 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    14461 ccggtggtcc atctggagca cggcgtcggt cgctatgccg gaatgaccac gctggaagct
    14521 ggcggcatta ctggcgagta cctgatgctc acctatgcca acgacgccaa actgtatgtt
    14581 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    14641 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    14701 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    14761 gcgtttaaac acgatcgtga gcagtatcag ttattctgcg acagcttccc gtttgaaacc
    14821 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    14881 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    14941 gctttcctgg cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt
    15001 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    15061 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    15121 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    15181 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    15241 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt
    15301 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    15361 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    15421 atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac
    15481 attcagaaag ccgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt
    15541 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    15601 ttcaacgtgc tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac
    15661 accattatca ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    15721 cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    15781 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    15841 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    15901 gaacaaagcg gttcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    15961 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    16021 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac
    16081 acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    16141 atcaaagtcg agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg
    16201 gatattgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    16261 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    16321 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    16381 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    16441 ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa
    16501 catttacaaa ttctttgcac tttcctgcac tatccgacac tgtcaccatc cataattcag
    16561 gcttatctgt ttattacaat aaccttatat ttattatgta tttttggtga tgatcaaaac
    16621 gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc
    16681 ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa
    16741 atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt
    16801 cggctttctc gctctgttac aagctaaccc cggcgttgat ccttacgtac cgcgcgcggg
    16861 cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt
    16921 gatcaacatt gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt
    16981 gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac
    17041 cgtttcagac aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta
    17101 taaagcacag ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg
    17161 ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc
    17221 cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat
    17281 caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat
    17341 aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga
    17401 gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt
    17461 taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg
    17521 gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa
    17581 aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg
    17641 agggttaatg cttatttata aattactgct gttccatgga gggtattcgg accggttaca
    17701 gaagtaatac ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc
    17761 agcgatccca gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct
    17821 ggcgttgact gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc
    17881 gcagcgatga ggtttttacg tttttcataa tagtggcctt atgcagatga atgacgtcgc
    17941 aagaaattac ttaacgatcg ataactaaat gatagatgtg atctggatca catacaagat
    18001 attttttata acaatcatta attaatttaa aaatctttaa atttcagtca aatagaaagc
    18061 aattattttt atgggatctg gactggtgaa cggaggtgat tatttttata ataacctttc
    18121 attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa
    18181 aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt
    18241 gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag caaccggagc
    18301 caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc gtgccgttct
    18361 cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa
    18421 aagcgtggag tctgcgctgg ctgactattt tgcggccatc gccaactgct ttaccagcaa
    18481 agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc cagattcagg
    18541 agatatagcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt
    18601 tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cacatctggc
    18661 agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct
    18721 ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac tggtgaaata
    18781 agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc caaattgcag agtacagcct
    18841 tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat ctgcgtaccc
    18901 ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa
    18961 ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa
    19021 gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc acccagcgcc
    19081 cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca
    19141 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
    19201 tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
    19261 gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact
    19321 gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc
    19381 acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct
    19441 atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc
    19501 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
    19561 gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt
    19621 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
    19681 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
    19741 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
    19801 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
    19861 accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
    19921 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
    19981 gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
    20041 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
    20101 taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
    20161 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
    20221 cgcagtagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
    20281 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc
    20341 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
    20401 cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg
    20461 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
    20521 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
    20581 acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
    20641 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
    20701 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
    20761 cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
    20821 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
    20881 gacaatcaca atctttttca atggcgtagt caacgtgacc cccttaaatt tattaaccaa
    20941 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
    21001 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
    21061 caagttttgt tgcttaaaag ataggtaact ttaccggggg agatcaaaaa atagagttta
    21121 ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat atcgagggcg
    21181 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    21241 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    21301 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    21361 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    21421 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    21481 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    21541 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    21601 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    21661 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    21721 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    21781 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    21841 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
    21901 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    21961 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    22021 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    22081 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    22141 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
    22201 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    22261 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    22321 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    22381 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    22441 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    22501 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    22561 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    22621 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    22681 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    22741 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    22801 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    22861 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    22921 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    22981 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    23041 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    23101 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    23161 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    23221 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    23281 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    23341 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    23401 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    23461 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    23521 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    23581 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    23641 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    23701 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    23761 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    23821 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    23881 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    23941 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    24001 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    24061 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    24121 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    24181 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    24241 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    24301 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    24361 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    24421 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    24481 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    24541 cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag
    24601 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    24661 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    24721 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    24781 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    24841 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    24901 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    24961 cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg ttggcgcttg
    25021 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    25081 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    25141 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    25201 ttaaacctgc tctttcaatc ttacctcacg atttcttaag caaaaaatcg ttttgctgcc
    25261 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    25321 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcat ttgcatttgc
    25381 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
    25441 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    25501 atcaagtcat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    25561 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    25621 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    25681 agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    25741 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    25801 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    25861 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
    25921 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    25981 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag
    26041 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    26101 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    26161 actcatggct ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg
    26221 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    26281 gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    26341 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    26401 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    26461 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    26521 tgctggttgg gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt
    26581 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    26641 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    26701 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
    26761 tttcccacaa gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa
    26821 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
    26881 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
    26941 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
    27001 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
    27061 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
    27121 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
    27181 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
    27241 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
    27301 tcttgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
    27361 aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa
    27421 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
    27481 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
    27541 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat
    27601 cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
    27661 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
    27721 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
    27781 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
    27841 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
    27901 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
    27961 tgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
    28021 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag
    28081 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    28141 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    28201 gtggatgatg tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat
    28261 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcacggtt
    28321 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    28381 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    28441 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    28501 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    28561 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    28621 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
    28681 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    28741 gaaacggtta aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt
    28801 atccgccaag tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    28861 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    28921 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    28981 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    29041 cgcggaaccg acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag
    29101 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    29161 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
    29221 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
    29281 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    29341 atatgtcaca atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac
    29401 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    29461 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    29521 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    29581 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
    29641 tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
    29701 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    29761 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    29821 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    29881 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
    29941 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
    30001 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    30061 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
    30121 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    30181 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    30241 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    30301 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt
    30361 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    30421 ccagttcggc gaccagtccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
    30481 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    30541 gattaagggc cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
    30601 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
    30661 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    30721 gtgaaagcca agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac
    30781 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
    30841 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    30901 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
    30961 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    31021 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    31081 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    31141 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    31201 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    31261 ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    31321 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    31381 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    31441 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa
    31501 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    31561 gccctgatac gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc
    31621 cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    31681 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    31741 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    31801 cagttgctcg agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    31861 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    31921 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    31981 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    32041 ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    32101 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    32161 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    32221 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    32281 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    32341 ctttcaccat tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat
    32401 acatcaaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    32461 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    32521 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    32581 cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    32641 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa
    32701 cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg
    32761 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    32821 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    32881 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    32941 atataacgtt gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat
    33001 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac
    33061 ttcgacaagc tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc
    33121 gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa
    33181 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    33241 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat
    33301 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc
    33361 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg
    33421 tccgctgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    33481 cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa
    33541 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa
    33601 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    33661 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    33721 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    33781 gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc
    33841 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    33901 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    33961 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    34021 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    34081 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    34141 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    34201 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    34261 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    34321 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    34381 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    34441 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    34501 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    34561 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    34621 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    34681 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    34741 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    34801 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    34861 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    34921 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    34981 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    35041 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat
    35101 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    35161 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    35221 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    35281 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    35341 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    35401 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    35461 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    35521 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    35581 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    35641 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    35701 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    35761 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    35821 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    35881 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    35941 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    36001 aaacagatga aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta
    36061 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    36121 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    36181 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    36241 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    36301 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    36361 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    36421 caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta
    36481 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    36541 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    36601 caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    36661 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt
    36721 ttgcgccagc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    36781 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    36841 cttcacacgc tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg
    36901 tgccttccgg tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat
    36961 cagcgaaatc aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    37021 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    37081 cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct
    37141 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    37201 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    37261 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag
    37321 agccccaagt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    37381 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    37441 ggcgatccag cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac
    37501 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    37561 tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    37621 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg
    37681 gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg
    37741 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    37801 cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    37861 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac
    37921 aggctgctgc aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac
    37981 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    38041 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    38101 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    38161 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    38221 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    38281 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    38341 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    38401 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    38461 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    38521 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    38581 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    38641 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    38701 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    38761 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc
    38821 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    38881 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    38941 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    39001 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    39061 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    39121 agggccatac gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    39181 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    39241 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    39301 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    39361 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    39421 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    39481 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    39541 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    39601 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    39661 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    39721 atacggcaaa tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg
    39781 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    39841 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    39901 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    39961 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    40021 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    40081 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    40141 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    40201 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    40261 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    40321 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    40381 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    40441 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    40501 acctgaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    40561 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    40621 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    40681 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    40741 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    40801 tcacctaagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc
    40861 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga
    40921 ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    40981 gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat
    41041 cggtgcagac ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    41101 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    41161 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    41221 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    41281 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    41341 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    41401 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    41461 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    41521 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    41581 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    41641 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    41701 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    41761 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    41821 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    41881 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    41941 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    42001 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    42061 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    42121 tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt
    42181 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    42241 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    42301 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    42361 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    42421 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    42481 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    42541 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    42601 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    42661 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    42721 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    42781 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    42841 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    42901 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    42961 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    43021 tatctggttc tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc
    43081 gatgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg
    43141 cttatcgtgc gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc
    43201 ttccgcctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    43261 ctgattcatc aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat
    43321 atcggcgaaa tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct
    43381 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    43441 ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg
    43501 ccatccggtg gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac
    43561 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    43621 gccgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    43681 gacttcatcg acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    43741 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    43801 gaaatgtccc gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg
    43861 cgctgctccg gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    43921 ttgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    43981 ccaatcgctt cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt
    44041 caggacggtg ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac
    44101 gtgttgcgcg tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg
    44161 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa
    44221 caacctgcac tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa
    44281 cctgccgcga ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct
    44341 gcacaacctg ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt
    44401 gaagaagcca aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa
    44461 caggatcgtc gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc
    44521 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    44581 gcacagcagc aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa
    44641 gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat
    44701 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    44761 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    44821 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    44881 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    44941 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    45001 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    45061 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    45121 atgccgttga ccgtagcgtg cacgtctccg gaattggcct ctggcaaagt ctggatccgc
    45181 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    45241 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    45301 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    45361 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    45421 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    45481 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    45541 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    45601 gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga tcaccgttga gcataaccac
    45661 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    45721 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    45781 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    45841 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata
    45901 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    45961 cgccccggcg atcccagagc gggcagacaa aacataccca gtttcatgat atgttttaaa
    46021 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    46081 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    46141 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc
    46201 cagcgactcc agtttgttca gctgcgtgcc taattccgcg cgaacggtaa ggacattatt
    46261 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    46321 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    46381 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    46441 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgctt
    46501 aatactttct gttccgccaa cataatcacc gttagcctca ctaaacggcg cagtctctgt
    46561 tttataaccg gcaaaaatgt agcgcccatt accgtcagtg gtgttcgcca gattcagcaa
    46621 ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt
    46681 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    46741 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca gcgtgtactg
    46801 gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcgtc
    46861 tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca tccattctgc
    46921 ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    46981 tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    47041 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    47101 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    47161 gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    47221 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    47281 agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc catcgcgatt
    47341 ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc
    47401 gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    47461 actttaccgt tggcatctgg tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc
    47521 tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccac ggaggcatca
    47581 gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc
    47641 ttaccgatag taaagaaatc ttcaccagca tcgccgttag catcaaatcc ggctttgtgt
    47701 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    47761 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    47821 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    47881 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    47941 ccattggcat cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa
    48001 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    48061 ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt
    48121 aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    48181 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    48241 cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    48301 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    48361 tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg
    48421 taataaacgt atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc
    48481 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    48541 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    48601 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    48661 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    48721 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt
    48781 gagtttacgg gcatagtgag gatcggtagc atagcccgcg tcctgtagga cctgcgcccc
    48841 ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc
    48901 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt
    48961 cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtgacttgcc ctttccagtt
    49021 gccagaggct ttgacaccaa acaggttata gcttggctcg ccgttttcgc ggcggatttg
    49081 ccgttgcccc caaccagatt caagcgcagc ctgagcgagg atcaaatgat gtggcacacc
    49141 gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctctactgtc
    49201 acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct gcgaaagcgt
    49261 ctggttttga taacgcacca cggtttcgag cgggaatttc atcggtgctg ctggcgtgga
    49321 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag
    49381 acctttgccc gccgtcatct gttgggcaat ttgctggtca tacatactgg tatacagacg
    49441 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    49501 catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc
    49561 ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    49621 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    49681 tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc attgagcgca
    49741 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    49801 cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc caccaccaaa cggtgtatct
    49861 ggctggctga cattggcctg acgattaact gttactgaga gatttccctg cgctaccgcg
    49921 cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact
    49981 actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaaacgg
    50041 acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg
    50101 gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa
    50161 tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca
    50221 cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg
    50281 ctaccgcctg cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac
    50341 acctgactgt caacgccctt aagcggtgtc atcaacaacg tacctccacg caagcttttg
    50401 gcattaccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga
    50461 agtgacgctg tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga
    50521 acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg
    50581 gttgtctggt caccagtgcc atccagcccc actaccaagc catagccaat cagtgagttt
    50641 tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta
    50701 accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg
    50761 acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat
    50821 tgcctacgta ttcaatacgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc
    50881 tgatagtgcg tggattaacc acgcccgaga agcgaataaa ttcggtaccc tgattaatgg
    50941 caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca
    51001 ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt
    51061 taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg
    51121 gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt
    51181 tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc
    51241 gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag
    51301 aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca
    51361 gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg
    51421 cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg
    51481 aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg
    51541 gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct
    51601 tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc
    51661 aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta gaggttttcg
    51721 ccaatgctct ccagtccggt atcattcata aaggtggtga gattgagctg cccaacctga
    51781 accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata
    51841 cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc
    51901 agctgcccgt tctgatccac ctggaaagag ccgtcacgga tataggctga tgagccatct
    51961 ggcaacatta cctggaaaaa gccctgccct ttaatcgcga catctttgct gttattggtc
    52021 tgcgacagat tgccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg
    52081 atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg
    52141 gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg
    52201 tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg
    52261 gcgatccata atgaactgat catataatgt cctgtagtgg attgctgaca agcagagcag
    52321 aagcacgcga cagtgtgaat ttcccctcac cctaaccctc tccccaaagg ggcgagggaa
    52381 tagtccgcgc gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt
    52441 ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac
    52501 gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca
    52561 gaacccccga catcacacgc aaggttggat ctgcctgcag taccggccct cgcgtggcct
    52621 gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg
    52681 ctttcaccag tttcagacgc cctactggcg caactgtatt tgccggatcg cccggattga
    52741 gcgccgagat tgtgccatcg gcagcaatcg tgatttccgc cccttccggc acagcaattg
    52801 gccccgcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg ggatcaacct
    52861 gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccatc
    52921 ctgctgcaac gcaacgtcca gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc
    52981 tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg
    53041 cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gtagaggcat tggccagatt
    53101 actggcggtt accgcctgtt gattcagcgt ctggctggct gctcccatcg cagtataaat
    53161 tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaacccgc gtgttgagga
    53221 tctggtcctg ggttttgatg gtctgggcgt tagacttata gttacgctgg gcaacgatca
    53281 tattgaccag ttctttactg agatcgacgt tggacgcttc cagcgcaccg ttggtcaggg
    53341 tgccaaagtt tcccgtcccg gctgtcccca acagcgccac gccagaagat tgcgtcgcag
    53401 accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc gccagtacaa
    53461 tctgccccag cagttgggtt tgttcgttgg aattgttgcc gacaaccgta ccgtcatcat
    53521 tgatttgata actcaccaga tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt
    53581 tgttagcgcc ggtattttgc tgcatggagt tgaggaagct cagactaaac gtggcgggtt
    53641 ctgcaccgtt aattgcgccg gttgcgatat tattcgccat cgcaccatcc actaatgtgc
    53701 cattagcatt aaattccagt gttgtcgctg tcttcgcaat gctgtttgga tcactgctat
    53761 cctgggtgta gacctgccag ttattatccc cggtcttcac aaaatagacg ctcatgtcat
    53821 gagcattacc ctgactgtcg aaaacagtca ccgaaccttt tttgttatag ctatccgcat
    53881 tgctggcgct gaatggcgta acagtaggaa gcggatcact ggaattcagg ttgatctgca
    53941 tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa atattggtcg
    54001 gattcgcccc ttgctgaata gtcggcggcg taccggttac cgggtaaccc gtcagctgta
    54061 aaccttgcgt attcaacagg ttacggtttt catccagctt aaattgtccg ttacggctgt
    54121 agaacaccga accgttgctg tctaccagac ggaaaaaacc gttctggctg atagcaacgt
    54181 ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa
    54241 cttttacccc cagtcccact ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg
    54301 atttaaagcc gtaggtggcg gagttggcga tattgttgcc aataacatcg aggttggtgg
    54361 cggcagcgtt taatccgcta accgcttgag aaaaggccat gactgactcc tgaaatgtga
    54421 aggcttaaat tatctgccgt acttcgtcga gggtggtggt gccgtaagtg ccgagatcca
    54481 gcgtattacc gctgttgccg cggatcacac cctgcaccag agcaaactgc agcggctggg
    54541 caaccagttg tgtaccaccg ttactggcgc taatcgctac attgtaagaa ccgttcggcg
    54601 cagtgctgcc atcagtcaac gaaccgtccc aggtgaaact gtgaactccg gcggtcagtt
    54661 caccaatatc aatggtgcgc acaaccgcgc catttttatc ggtgatggtg gccgtaactt
    54721 tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt cgtggtcaca gccccttctt
    54781 cactgccggt tcccgcaaga acagttgtgc cggggatcat cacgccgtga ccgatcaggt
    54841 tactggcctg taacgactgg ctgttatcaa tctgtccgga aatagatccg agcgtggtat
    54901 tgagtttttc aatcccgctg accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt
    54961 tttccattgg attggtcggg tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac
    55021 tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact actgctggtg gtactgacgc
    55081 cggtatttgt cggatcggtg gtggttaccg caatggacat agctttctcc tttattgacc
    55141 gagcgtaagg gttttcagca tcatgctttt caccgtgttg agcacttcaa cattggcctg
    55201 atagctgcgt gacgccgaca tggtgttaac catctccccg acgacatcaa cgttcggcat
    55261 ttttacgtag ccctttgcat ctgccagcgg attacctggt tcataaacca gtttgtccgg
    55321 ggcctgactt tcttaacatc ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg
    55381 ttaacctgga ataccacctg ttttgcccga tatggctgtc catcaggacc ggtcacgcta
    55441 tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc agttaacgcc
    55501 gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct ctgtaaaacg
    55561 ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta ttgcaggctg
    55621 ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc
    55681 tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg cgccgggatg
    55741 tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc acgttgcatg
    55801 acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg ggtatcggca
    55861 ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag cgcctcttgt
    55921 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    55981 tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    56041 gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta gaaatggctg
    56101 ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg
    56161 ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg
    56221 ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact accgccatgc
    56281 gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg
    56341 gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat
    56401 gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaactg
    56461 aaacgtggac gcctggatac cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt
    56521 gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccagtttcgc
    56581 caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt
    56641 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg
    56701 cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa tattcttata
    56761 aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    56821 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagcctgta
    56881 agcaccgttc aaccgcgcga aaccaccgac gcgccggtaa cgaacagccg ggcggcaaaa
    56941 acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    57001 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac
    57061 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa
    57121 gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc agatgtccgc
    57181 tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct ctatggggca
    57241 gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc tggcgacgct
    57301 ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg ttgatattag
    57361 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    57421 cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa tgttgaaacc
    57481 gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacacccc atcgcggcag
    57541 taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga atgcattgtt
    57601 acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt gcagcgaagt
    57661 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag ggcatggtac
    57721 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    57781 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta aagattttct
    57841 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    57901 gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    57961 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa taaaagccag
    58021 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    58081 gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc gaaacggtta
    58141 acggacctga aagaatgccc aacgcaaccg cactcggcag tgccaacagg aaacaaagac
    58201 gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    58261 acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag ggaaactcca
    58321 ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa
    58381 tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc ggtcccatct
    58441 gtttcaccac gcgcattgct ccagcatcgt ggaagttaat gcgcggcagg accagcatgc
    58501 cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg accgtaacag
    58561 cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa
    58621 tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg ttccacgtat
    58681 tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga aaggtaatct
    58741 ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca
    58801 ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg
    58861 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt
    58921 tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    58981 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    59041 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    59101 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccgg acccacgggc
    59161 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctagatt atggtgaatt
    59221 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    59281 atacgttgcg ccagcactgc ctattcgccg gcggtttgcg gaactgtctg attttgcacg
    59341 cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt gctctgccaa
    59401 ccaggaatcg gtttattcac tacaccctgc ccgcgctcct cacgccattc gcgcatatcc
    59461 gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg acttccggca
    59521 cggcgatgca tacaagtggt gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac
    59581 agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc tttgccgccc
    59641 gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt gaaataaaga
    59701 tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc tgcggtggcg
    59761 agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    59821 gtcagttttt tccgcgccgc cagttccacc agcctttcag catcgcgcag attttctgcc
    59881 agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac cacgtcaaag
    59941 tggctggcgg tgctggagtg cacaaaaacc gcatcacaac tggcggcaag gctggataac
    60001 gaatcggcat aaggaatgcg ccagctgtca caaattggca gggctttggc gcgcgtaggc
    60061 gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    60121 tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt ttttcacttt aatctcccag
    60181 atgggccagc aacgaatcaa gacgttgttt cagttctgcc acttcgattt ccagggcttc
    60241 gacgcgggtc tataaatcac cgtcaaccat gttcgacata gccgttaccg ccggctgatc
    60301 ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    60361 cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg tcgactccac
    60421 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt
    60481 ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc tcagtttcag
    60541 atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat taccaaaacc
    60601 gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct gcacttcgga
    60661 ttcactcaga ttcatgaccg gtttacggtt cgttttctga ttacaggccg tgactacacc
    60721 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    60781 tacgcgagct tccaaggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    60841 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    60901 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc
    60961 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    61021 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    61081 tcgaataacc gagatagcag gcatgaaaag agccgcgaac aacattggaa aaattggcaa
    61141 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct gaaccttgtt
    61201 tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa cagtggcttt
    61261 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcca
    61321 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    61381 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    61441 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc
    61501 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac gttttgataa
    61561 aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    61621 tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta
    61681 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    61741 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc
    61801 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    61861 gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc ggattcgcca
    61921 caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc tcgggactcg
    61981 gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac
    62041 aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccagtgcg gtcattggcg
    62101 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggccg
    62161 ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    62221 ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca
    62281 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    62341 caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg tatgccattg
    62401 aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa aagcattttc
    62461 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    62521 gcatggtcag cggcctacaa caacttttca ccctgatttg tctgttttat atcgggtcga
    62581 tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg
    62641 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    62701 gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt ccgtggatga
    62761 tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agtcagaaac
    62821 gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc tgactcctcc
    62881 atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac atttacgatc
    62941 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    63001 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    63061 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat atcgttcact
    63121 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    63181 agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt tttgccaaat
    63241 cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac aagaaagagg
    63301 ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    63361 gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    63421 tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg gtagccggaa
    63481 tcaactggcc aagccgcgtt gctaattacc gcgataatat ggcgaaacag acacaaatca
    63541 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    63601 tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca tgctgcgttg
    63661 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    63721 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    63781 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    63841 ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca aattggtcgc
    63901 gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa ctccctgttc
    63961 ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa
    64021 aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca accggaaaaa
    64081 atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg caattacttt
    64141 aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa
    64201 aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt tacggattaa
    64261 tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc
    64321 tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac tgcaccatcc
    64381 caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga tggcgacatg
    64441 ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt aatgcccaat
    64501 ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg tattcttgac
    64561 gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac agattcgctg
    64621 gatcctaatg agctggagcg cggatttaat gaagccgtgt tcaccgctgc aaaactttac
    64681 ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc aatcatgccg
    64741 gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga agtgacacat
    64801 gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg
    64861 cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc
    64921 gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca gcatctgatg
    64981 tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg tttacccatc
    65041 ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg ttttaatcga
    65101 gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag cagttgcggc
    65161 tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag
    65221 atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca gttctatggc
    65281 ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt tgctgaaagc
    65341 atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt acgctggtcc
    65401 gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct gtataaataa
    65461 ccagtatgtt caacaggggg ctattatgcg aattgaagtc accatagcga aaacttctcc
    65521 attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta ttcagtatgc
    65581 gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata atttatcggt
    65641 tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg aaacgtggga
    65701 aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt ttgccggatc
    65761 gccccggctt ttttatttta cttacgaatt ttgagtacaa aatttctaca cttcctcaaa
    65821 ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt tcatataaat
    65881 cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac ccttacttat
    65941 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
    66001 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
    66061 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
    66121 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca
    66181 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
    66241 gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
    66301 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
    66361 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
    66421 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
    66481 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
    66541 gccatgctta tggtgaaggc gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt
    66601 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
    66661 acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
    66721 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
    66781 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg
    66841 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc
    66901 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt
    66961 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc
    67021 tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg
    67081 tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt
    67141 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
    67201 cagcggatga acgtgttccg tttgcggaag tggccagtga tgggtcagaa gccttcccgt
    67261 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg
    67321 ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc
    67381 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
    67441 ccgattttgc gcaagaccaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
    67501 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
    67561 tcggctgttg cactatctca tcaataatat tcgcgagcat ttgatgcttt atctttttct
    67621 ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact
    67681 gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgatatt
    67741 attcctgctt ctacagcact caccagacat ctctgatgct gaacccacta aacttatgaa
    67801 gattctttca ttattaattc aaaattattg aaataaaaat aaatatcatt caatttgtta
    67861 tttttgtgtt gcaattcatt ctccgacacg ccattttctt tctctcgcaa attcgtattg
    67921 gatgcactta tgtcattcac aaatacccct gaacgctatg gtgttatatc tgcggccttc
    67981 cattggctaa gcgcaattat tgtctacggc atgtttgctt taggtctgtg gatggtcacg
    68041 ctcagttatt acgatggctg gtatcacaaa gcacccgaac tgcataaaag catcggtatt
    68101 ttgttaatga tggggctggt tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt
    68161 ccgctgccga gttattcgcc catgactcgt cttgccgcaa aagctggaca tctcgcattg
    68221 tacctattgc tgttcgccat cggcatcagc ggctatctga tctcaactgc cgatggtaaa
    68281 ccgatcagcg tttttggctg gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag
    68341 gcagactttg ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt actgtctgtt
    68401 atgcacggat ttatggccct gaagcatcat ttcatcgata aagacgacac tctgaagcgc
    68461 atgctgggaa agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt
    68521 ttaaccttcg cgtccctgat gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt
    68581 gacaaagaag gtcagcacgc ctttgttaac ttccgcatac agcatcttgg ctatagctgg
    68641 ttatatggca cctttaaaga tttcgacggt acttttacct ttgatgaaaa aaatccggct
    68701 gccgataaag tgaatgtgac aattaacacc accagcgtcg atactaacca cgccgaacgc
    68761 gataaacatc ttcgcagtgc ggattttctc aataccacaa aatatccaca ggcaacattc
    68821 acctccacca gcgtgaagaa agacggtgac gaactggata ttaccggcga tctgacgctg
    68881 aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca
    68941 tggggcggta aacgtgcagg ctttgaggcc gaaggcaaaa ttaagctcaa agacttcaat
    69001 atcaagacag atttaggtcc agcttctcag gaagtggatc tgattatttc agtggaaggc
    69061 gtacagcaga agtaagagtg aaatgttgcc ggatggcacg catccggcaa tgatatttat
    69121 tctgttggat caggaatacc cagtgttgta ttcagacgtc cacgagactt attaaagatc
    69181 ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt
    69241 tcttcgcagc aaatctcact acaacaacct ttgtattttt ccgcgcatac tggacactga
    69301 ataaacagca ggtggcagcc atcattttta cagttggtat ggctgtcgca cggcgcgccg
    69361 cactggtggc aatgcgcgat aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa
    69421 acaaaatttt tgccaataaa acgcaccggc aagccctgct cgcgcgcctt acgggcgtat
    69481 tcaataattc cgccctcgat atgccagact ttgttgaatc cgttatgttt catccaggca
    69541 ctggcctttt cacaacgaat gccgccggtg cagtacatga cgattttttt atctttatgt
    69601 gcctgcatca tctcgactgc ttttggcagc tgctcacgga aggtatctgc cggaatttcg
    69661 agcgcgtttt caaagtgccc cacttcatac tcatagtggt tacgcatgtc gataaatagt
    69721 gcatcgggat cgtcaagcat ggcgttcact tccgccgctt gcagatactc accaacattg
    69781 ctggcatcaa agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg
    69841 cgcagtaccc agaaggattt cccgtcatca tccaacgcga tattcaggcg taaaccctct
    69901 aaagccgggt cgaaggcata gagctgcgcg cgaaatgttt caacattgct cgcaggtacg
    69961 ctgatttgcg cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac attcagcgcg
    70021 gtaaacagct gatataaagc gtcacgggtc gccttaggat cggcgatgtg gaaatacttg
    70081 taaaacgaaa tggtggttcg cggttcgctc tcagccaaca ttttggcttt taacgcgtcg
    70141 ttggaaatgc ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa
    70201 aaatccgcgg catgatatag caattatcga taattaacat ccacacattt tacgctacat
    70261 ttgcgcatta aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagcca
    70321 caaattttgg ttgcgggcga aaaaatgcga caatacatac aattgcccgt ataggttgaa
    70381 aaacaggatt gatatgacga atctacccaa gttctccacc gcactgcttc atccgcgtta
    70441 ttggttaacc tggttgggta ttggcgtact ttggttggtc gtgcaattgc cctacccgat
    70501 tatctaccgc ctcggttgtg gattaggaaa actggcgtta cgttttatga aacgacgcgc
    70561 aaaaattgtg catcgcaacc tggaactgtg cttcccggaa atgagcgaac aagaacgccg
    70621 taaaatggtg gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa ccggtatggc
    70681 gtggttctgg ccggaccgcc gaatcgcccg ctggacggaa gtgatcggca tggaacacat
    70741 tcgtgacgtg caggcgcaaa aacgcggcat cctgttagtt ggcatccatt ttctgacact
    70801 ggagctgggc gcgcggcagt ttggtatgca ggaaccgggg attggcgttt accgcccgaa
    70861 cgataatcca ctgattgact ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat
    70921 gctcgaccgc aaagatttaa aaggcatgat taaagccctg aaaaaaggcg aagtggtctg
    70981 gtacgcacca gatcatgatt acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt
    71041 tgagcaggct gcgaccacga ccggaacctg gatgctggca cggatgtccg gcgcatgtct
    71101 ggtgcccttc gttccacgcc gtaagccaga tggcaaaggg tatcaattga ttatgctgcc
    71161 accagagtgt tctccgccac tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt
    71221 ggtcgaaaaa tgcatcatga tggcaccaga gcagtatatg tggttacacc gtcgctttaa
    71281 aacacgcccg gaaggcgttc cttcacgcta ttaaatctcc catgccggat gcttcagaat
    71341 ggcatccggc attaccacag caaatccccc tgatttagcg ataaaagctc tctggattgc
    71401 gccccctgga agtcgggcgc ataattagtg tgtttatctt ttcttcttat gttcaccgcg
    71461 cctggcgcac caacagcgga ttgctatgtc accctgtgaa aatgacaccc ctataaactg
    71521 gaaacgaaac ctgatcgtcg cctggctagg ctgttttctt accggggccg acttcagtct
    71581 ggtaatgccc ttcttacccc tctacgttga gcagcttggt gttaccggcc actccgccct
    71641 gaatatgtgg tccggtattg tcttcagtat aatgggttcc taaacacatc attttaggta
    71701 ttgaatatga gacgggcaat cagctacatc agattttcaa gtgaacgaca actgaaaggg
    71761 gactctgtac gccgacaatc taagctggtt actgactggc ttgataagaa ccctgggttt
    71821 tacttggatt catccttgag ctttaaagat ttggggaaaa gtgcttttag tggtaagcac
    71881 ctgaaaggtg gtctaggtga ctttttaacc gctatcgaga aagggcttgt taaagctggt
    71941 gatacgctac taatcgagag tttagaccgt ctgtcacgtc aggatattga tattgcttct
    72001 gagctattac gccgtatctt aagggctggt gtagatgtag tcacactgag cgatggagaa
    72061 cattacacac gagaaagttt aaaagacccg ttagccctga ttaaaagcat tctgataatg
    72121 cagcgggcgc atgaggaaag tttgagaaag tctgagcgtg tccaagctgc atggaaccgt
    72181 aaaaaagaac tcatctccga aggtatcaag gtatc
//
LOCUS       DAEEDS010000006        72081 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEEDS010000006 DAEEDS010000000
VERSION     DAEEDS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72081
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.006"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1334..2929)
                     /locus_tag="KLH32_000420"
     CDS             complement(1334..2929)
                     /locus_tag="KLH32_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBE0137393.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3284..5455)
                     /gene="glcB"
                     /locus_tag="KLH32_000421"
     CDS             complement(3284..5455)
                     /gene="glcB"
                     /locus_tag="KLH32_000421"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBE0137394.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5477..5881)
                     /locus_tag="KLH32_000422"
     CDS             complement(5477..5881)
                     /locus_tag="KLH32_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBE0137395.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5886..7105)
                     /gene="glcF"
                     /locus_tag="KLH32_000423"
                     /pseudo
     CDS             complement(5886..7105)
                     /gene="glcF"
                     /locus_tag="KLH32_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7116..8168)
                     /gene="glcE"
                     /locus_tag="KLH32_000424"
     CDS             complement(7116..8168)
                     /gene="glcE"
                     /locus_tag="KLH32_000424"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBE0137396.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8168..9667)
                     /gene="glcD"
                     /locus_tag="KLH32_000425"
                     /pseudo
     CDS             complement(8168..9667)
                     /gene="glcD"
                     /locus_tag="KLH32_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9918..10688
                     /gene="glcC"
                     /locus_tag="KLH32_000426"
     CDS             9918..10688
                     /gene="glcC"
                     /locus_tag="KLH32_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBE0137397.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10812..11953)
                     /locus_tag="KLH32_000427"
                     /pseudo
     CDS             complement(10812..11953)
                     /locus_tag="KLH32_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12315..14045
                     /locus_tag="KLH32_000428"
     CDS             12315..14045
                     /locus_tag="KLH32_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBE0137398.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14042..14956
                     /locus_tag="KLH32_000429"
     CDS             14042..14956
                     /locus_tag="KLH32_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBE0137399.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14989..15237
                     /locus_tag="KLH32_000430"
     CDS             14989..15237
                     /locus_tag="KLH32_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE0137400.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15237..16408
                     /locus_tag="KLH32_000431"
                     /pseudo
     CDS             15237..16408
                     /locus_tag="KLH32_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16444..17523)
                     /gene="lptG"
                     /locus_tag="KLH32_000432"
     CDS             complement(16444..17523)
                     /gene="lptG"
                     /locus_tag="KLH32_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBE0137401.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17520..18590)
                     /gene="lptF"
                     /locus_tag="KLH32_000433"
     CDS             complement(17520..18590)
                     /gene="lptF"
                     /locus_tag="KLH32_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBE0137402.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18621..19181)
                     /locus_tag="KLH32_000434"
     CDS             complement(18621..19181)
                     /locus_tag="KLH32_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBE0137403.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19193..20017)
                     /locus_tag="KLH32_000435"
     CDS             complement(19193..20017)
                     /locus_tag="KLH32_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137404.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20017..21366)
                     /locus_tag="KLH32_000436"
     CDS             complement(20017..21366)
                     /locus_tag="KLH32_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBE0137405.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21412..22170)
                     /locus_tag="KLH32_000437"
     CDS             complement(21412..22170)
                     /locus_tag="KLH32_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137406.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22202..22915)
                     /locus_tag="KLH32_000438"
     CDS             complement(22202..22915)
                     /locus_tag="KLH32_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE0137407.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23089..23781
                     /locus_tag="KLH32_000439"
     CDS             23089..23781
                     /locus_tag="KLH32_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE0137408.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23830..25329)
                     /gene="pitB"
                     /locus_tag="KLH32_000440"
     CDS             complement(23830..25329)
                     /gene="pitB"
                     /locus_tag="KLH32_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBE0137409.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25621..27480)
                     /gene="gss"
                     /locus_tag="KLH32_000441"
     CDS             complement(25621..27480)
                     /gene="gss"
                     /locus_tag="KLH32_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBE0137410.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27685..28551
                     /gene="yghU"
                     /locus_tag="KLH32_000442"
     CDS             27685..28551
                     /gene="yghU"
                     /locus_tag="KLH32_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBE0137411.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHELHDAS
                     DFETNTEDKRQG"
     gene            complement(28674..28922)
                     /gene="hybG"
                     /locus_tag="KLH32_000443"
     CDS             complement(28674..28922)
                     /gene="hybG"
                     /locus_tag="KLH32_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBE0137412.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29056..29397)
                     /gene="hypA"
                     /locus_tag="KLH32_000444"
     CDS             complement(29056..29397)
                     /gene="hypA"
                     /locus_tag="KLH32_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE0137413.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29390..29878)
                     /gene="hybE"
                     /locus_tag="KLH32_000445"
     CDS             complement(29390..29878)
                     /gene="hybE"
                     /locus_tag="KLH32_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBE0137414.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29871..30365)
                     /locus_tag="KLH32_000446"
     CDS             complement(29871..30365)
                     /locus_tag="KLH32_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBE0137415.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30365..32068)
                     /gene="hybC"
                     /locus_tag="KLH32_000447"
     CDS             complement(30365..32068)
                     /gene="hybC"
                     /locus_tag="KLH32_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBE0137416.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32065..33243)
                     /gene="hybB"
                     /locus_tag="KLH32_000448"
     CDS             complement(32065..33243)
                     /gene="hybB"
                     /locus_tag="KLH32_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBE0137417.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33233..34219)
                     /gene="hybA"
                     /locus_tag="KLH32_000449"
     CDS             complement(33233..34219)
                     /gene="hybA"
                     /locus_tag="KLH32_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBE0137418.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34222..35340)
                     /gene="hybO"
                     /locus_tag="KLH32_000450"
     CDS             complement(34222..35340)
                     /gene="hybO"
                     /locus_tag="KLH32_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBE0137419.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35529..35816)
                     /locus_tag="KLH32_000451"
     CDS             complement(35529..35816)
                     /locus_tag="KLH32_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBE0137420.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35935..36822)
                     /locus_tag="KLH32_000452"
     CDS             complement(35935..36822)
                     /locus_tag="KLH32_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE0137421.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36979..38019
                     /gene="gpr"
                     /locus_tag="KLH32_000453"
     CDS             36979..38019
                     /gene="gpr"
                     /locus_tag="KLH32_000453"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBE0137422.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38059..38553)
                     /locus_tag="KLH32_000454"
     CDS             complement(38059..38553)
                     /locus_tag="KLH32_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBE0137423.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38744..39628
                     /gene="yghA"
                     /locus_tag="KLH32_000455"
     CDS             38744..39628
                     /gene="yghA"
                     /locus_tag="KLH32_000455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE0137424.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39900..40325)
                     /gene="exbD"
                     /locus_tag="KLH32_000456"
     CDS             complement(39900..40325)
                     /gene="exbD"
                     /locus_tag="KLH32_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBE0137425.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40332..41066)
                     /gene="exbB"
                     /locus_tag="KLH32_000457"
     CDS             complement(40332..41066)
                     /gene="exbB"
                     /locus_tag="KLH32_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE0137426.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41318..42505
                     /gene="metC"
                     /locus_tag="KLH32_000458"
     CDS             41318..42505
                     /gene="metC"
                     /locus_tag="KLH32_000458"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBE0137427.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42645..43304
                     /locus_tag="KLH32_000459"
     CDS             42645..43304
                     /locus_tag="KLH32_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0137428.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43344..44243)
                     /gene="yqhC"
                     /locus_tag="KLH32_000460"
     CDS             complement(43344..44243)
                     /gene="yqhC"
                     /locus_tag="KLH32_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBE0137429.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44437..45600
                     /gene="yqhD"
                     /locus_tag="KLH32_000461"
     CDS             44437..45600
                     /gene="yqhD"
                     /locus_tag="KLH32_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBE0137430.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45705..46532
                     /gene="dkgA"
                     /locus_tag="KLH32_000462"
     CDS             45705..46532
                     /gene="dkgA"
                     /locus_tag="KLH32_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBE0137431.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46732..47658
                     /locus_tag="KLH32_000463"
     CDS             46732..47658
                     /locus_tag="KLH32_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBE0137432.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47709..47966
                     /gene="yqhH"
                     /locus_tag="KLH32_000464"
     CDS             47709..47966
                     /gene="yqhH"
                     /locus_tag="KLH32_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBE0137433.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48009..50228)
                     /locus_tag="KLH32_000465"
     CDS             complement(48009..50228)
                     /locus_tag="KLH32_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBE0137434.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50339..51751)
                     /gene="ftsP"
                     /locus_tag="KLH32_000466"
     CDS             complement(50339..51751)
                     /gene="ftsP"
                     /locus_tag="KLH32_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBE0137435.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51826..52563)
                     /gene="plsC"
                     /locus_tag="KLH32_000467"
                     /gene_synonym="parF"
     CDS             complement(51826..52563)
                     /gene="plsC"
                     /locus_tag="KLH32_000467"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBE0137436.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52797..55055)
                     /gene="parC"
                     /locus_tag="KLH32_000468"
     CDS             complement(52797..55055)
                     /gene="parC"
                     /locus_tag="KLH32_000468"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBE0137437.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55601..56083)
                     /locus_tag="KLH32_000469"
     CDS             complement(55601..56083)
                     /locus_tag="KLH32_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBE0137438.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56136..56528)
                     /gene="ygiW"
                     /locus_tag="KLH32_000470"
     CDS             complement(56136..56528)
                     /gene="ygiW"
                     /locus_tag="KLH32_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBE0137439.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56680..57339
                     /gene="qseB"
                     /locus_tag="KLH32_000471"
     CDS             56680..57339
                     /gene="qseB"
                     /locus_tag="KLH32_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBE0137440.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57336..58610
                     /gene="qseC"
                     /locus_tag="KLH32_000472"
     CDS             57336..58610
                     /gene="qseC"
                     /locus_tag="KLH32_000472"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBE0137441.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58656..58988)
                     /locus_tag="KLH32_000473"
     CDS             complement(58656..58988)
                     /locus_tag="KLH32_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBE0137442.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59307..59888
                     /gene="mdaB"
                     /locus_tag="KLH32_000474"
     CDS             59307..59888
                     /gene="mdaB"
                     /locus_tag="KLH32_000474"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBE0137443.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59919..60233
                     /locus_tag="KLH32_000475"
     CDS             59919..60233
                     /locus_tag="KLH32_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBE0137444.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60281..62173)
                     /gene="parE"
                     /locus_tag="KLH32_000476"
     CDS             complement(60281..62173)
                     /gene="parE"
                     /locus_tag="KLH32_000476"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBE0137445.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62202..62783)
                     /gene="yqiA"
                     /locus_tag="KLH32_000477"
     CDS             complement(62202..62783)
                     /gene="yqiA"
                     /locus_tag="KLH32_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBE0137446.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62783..63610)
                     /gene="cpdA"
                     /locus_tag="KLH32_000478"
     CDS             complement(62783..63610)
                     /gene="cpdA"
                     /locus_tag="KLH32_000478"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBE0137447.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63635..64057)
                     /locus_tag="KLH32_000479"
     CDS             complement(63635..64057)
                     /locus_tag="KLH32_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBE0137448.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64058..64687)
                     /gene="nudF"
                     /locus_tag="KLH32_000480"
     CDS             complement(64058..64687)
                     /gene="nudF"
                     /locus_tag="KLH32_000480"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBE0137449.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64892..66373
                     /gene="tolC"
                     /locus_tag="KLH32_000481"
     CDS             64892..66373
                     /gene="tolC"
                     /locus_tag="KLH32_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBE0137450.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66521..67192
                     /locus_tag="KLH32_000482"
     CDS             66521..67192
                     /locus_tag="KLH32_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBE0137451.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67198..68358
                     /locus_tag="KLH32_000483"
     CDS             67198..68358
                     /locus_tag="KLH32_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE0137452.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68396..69184)
                     /gene="ygiD"
                     /locus_tag="KLH32_000484"
     CDS             complement(68396..69184)
                     /gene="ygiD"
                     /locus_tag="KLH32_000484"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBE0137453.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69327..70100
                     /gene="zupT"
                     /locus_tag="KLH32_000485"
     CDS             69327..70100
                     /gene="zupT"
                     /locus_tag="KLH32_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBE0137454.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70158..70328)
                     /locus_tag="KLH32_000486"
     CDS             complement(70158..70328)
                     /locus_tag="KLH32_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137455.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70589..71242)
                     /gene="ribB"
                     /locus_tag="KLH32_000487"
     CDS             complement(70589..71242)
                     /gene="ribB"
                     /locus_tag="KLH32_000487"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBE0137456.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71346..71494)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71616..71906
                     /gene="ubiK"
                     /locus_tag="KLH32_000488"
     CDS             71616..71906
                     /gene="ubiK"
                     /locus_tag="KLH32_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBE0137457.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18029 a        19165 c        17743 g        17144 t
ORIGIN      
        1 tacgtaccgt ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt
       61 acagggctta ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca
      121 taagaaatca aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt
      181 cattaatact gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga
      241 gatccatcgg tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg
      301 tatcagattc ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat
      361 ttacattatt tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt
      421 aatctcttac tactttcgtg ttattgcaat acaaccatcc gtaaagcagg gctattttta
      481 gcaggaaata ctcaggtgtc aacattcgaa ctccaactct gaaatattca tataagttaa
      541 tgattttaat gattttaatg attttaatga ttttaatgat tttttcattt attcacacct
      601 aataaaagta ttcaaattca gatgattttt ctgctcaaca gacacaaaca ggcaataata
      661 ctctccaaag cgaacaatat ttggcattac gatttaatgc ttcataatca ccaaattatt
      721 taaccataaa ttttcttatt tctaacaaaa tgattggtgt gttgttggag ttaaaataca
      781 tacataatat taacatctgc caatatttca ctttccgcaa gacggatgat ttcgaatcct
      841 catccacgtt accctatccc aaatatcttt attaataggc agtaacgaac actgttatag
      901 caagattaac ttattaacct aacggcatct tttatgtaac aagtggagat aaattgctta
      961 aaattgatcc tcgacaaatc ttaaaagttc tcattattct gcaaaatcag ctttgcgaat
     1021 aatcttatgt ttttgggagt tttgggaatt ttgatctact cattgttcta gtgaaaaata
     1081 taaataagag aaaaaacgcc attcgtcatg aaagcaacag atgaagcaat gtaataacat
     1141 actaaaattg atctcatgaa agtgggtact ttataaccag cacagaagcc aagttgtata
     1201 aatattaaac aattcgcatc gtaaaacgga gactacgtat aaagaagcca atcgtaaaaa
     1261 cggttccctg gcgagataaa cgacagacaa atggaaagcc gggtgatatt taccttcatc
     1321 cggctttatt gatttacgag actaacatcc cggtaaacac atacgcctgc agcagggtga
     1381 taacgccgat aacgctggca aaaatcagac tgtgcttcac ggtgtagcgg aacagttcag
     1441 attctcggcc caccatgccc gtcgcggcgc aggccacggc gatagattgc ggggagatca
     1501 tcttgccagt tacgccgccg ctggtatttg ctgccaccag cagagtgtca gagacgttga
     1561 tttgctgcgc cgtggtcgat tgcagtgaac caaacagggc gtttgaagaa gtgtccgacc
     1621 cggtaaggaa tacgcccagc cagccgagaa acggtgagaa aaacgggaac atcacgcctg
     1681 tacctgccag taccagcgcc agcgtggtgg acatgccgga atagttggtg acgaaggcga
     1741 atgccagcac catgccaatc gacaatatcg gccacttcag actaattagc atttcggcaa
     1801 agacgccaat ccctttcttg atgcccacgc cgaggatgaa gatagagata atcgccgcaa
     1861 taaaaatagc ggtgccgcca gccgagagag ggtcgaattt aaacaccgca tccattggcg
     1921 ttggttgggc gacaatgggt gccgctttca acacttgttg atgcaaatga gggatctgga
     1981 aattaatcac cagtgaataa aacgcgccgc ccggagcaaa taacgcttta aacggcttca
     2041 tggtccagat ggtgaccagc accgttaaga ttaaaaacgg tgaccacgca cgaatgattt
     2101 gccccagact atattctgaa ggcacgggac cgccagaaga tggcttattt accaccatcg
     2161 cacctgcgga ttgccccatg ctgattgccg tttcggtatt tttcggccgc cagactttaa
     2221 gaaataaagc gagtgagacg atactcacca gcgccgaagt aatatccggc agttccggac
     2281 caatatagtt agaggtaaag aactgagtga cagcgaagct tcccccagca accagcgccg
     2341 ctggccacgt ctctttcacc cctttccagc cgtccatcat tgctaccagc cagaacggca
     2401 caagaaccga caggaacggt aactgacgtc ccgccattgc gccaatgtgg aacggatcga
     2461 ttcccgttac ctgaccggcg accagaatcg gcacgcccaa cgcaccaaac gccaccgtcg
     2521 caccaaagcc agcagctcct tccagcaacg caccaaagga gaaaccaatc agtaacacct
     2581 gcaaacgctg atcgtcggtg atggagataa ccaagctgcg gataatatcg aactgcccgc
     2641 tggcaacggt taatttatac aggaacaccg ccgcgacaat aatccacgca attggccata
     2701 atccataaat aaagccatag cccgcagcag caaatgccat atcaatcggc attttaaagg
     2761 cgaatatcgc aatcaggata gataatataa gggttattgc tccagcgaca tgtcctttca
     2821 gacgtaatac cgcgagtgca acgaagaaga atattatcgg gatcagggcg accagagcgg
     2881 atagccccag tcctcccatc ggcatataca tttgggtcca ggtaaccata ttgttcagtc
     2941 tcttattatc tttatatgct tgatatactt aaggttgtaa taagcaaaag aggactgaac
     3001 tgtaaaatat aggcgttata ctttacagca acagtacgcc gctaacgcaa ttgctacctc
     3061 tggcataaca agtatatcgg gtaagggttt ctgttccgca cacgcaggcg cagagtatcg
     3121 ttaagatgtc catattgttg ttttaggccc gctagtaatg cgctacgggt atttaatatt
     3181 gttaaaccct gataatcgct ccggttattt ccgggataaa tgtactaccg cagttactat
     3241 catagccccg acaataaaac ttgccggggc ttttttgacg ctattaatga ctttcttttt
     3301 cgcgtaaacg ccaggcgtgt aataacggtt cggtataacc gtttggctgt ttcacgccga
     3361 ggaagattaa atcgctggca gctttaaaag cacacgagtt agcgaaattc cccgccatcg
     3421 gacgataagc cggatcgcca gcattttgct gatcaaccac tttcgccata ttctccagcg
     3481 acgcctgcac ctgttctttg gtcagaatac cgtgacgtaa ccagttggcg atatgctggc
     3541 tggagatacg cagcgttgcg cggtcttcca tcagcgccac gttgtgaata tccggcactt
     3601 ttgaacaacc aatcccctgc tccacccaac gcaccacgta ccccagaatc ccctgcacgt
     3661 tgttatccag ctcttgttgg atctcttgcg ccgaccagtt agcgttttca gccaccggaa
     3721 tagtcagcag atcgtccagc agcggttcaa attcagcact gaactcggtc tgggcaatgt
     3781 tggcttgcac gctctgtacg ttggtatggt ggtagtgcag cgcatggagc gtagcagcgg
     3841 ttggtgacgg aacccaggct gtgtttgccc cggcacgcag ttggtcgccc ttctggctgt
     3901 acatgtctgc catcaggtcc ggcattgccc acatgccttt accgatttgc gctttaccgc
     3961 gcagcccaca gaacagaccg gaaagcacgt tattacgttc gtaggctttg atccacggcg
     4021 tcgatttcat ctggttttta cgcagcatcg ggccagcttc catcaccgaa tgcatttcat
     4081 cgccggtacg gtcgaggaaa ccggtattga tgaacgccac gcggttgcgc gcctgagcga
     4141 tacagctacg caagttcagc gaggtccggc gttcttcatc cataatgccc attttcaggg
     4201 tattcggtgc cataccgagc attgtctcaa tgcgggtaaa cagtttgttg gcgaacgcca
     4261 cttcctgcgg accgtgcatt ttcggtttca caatatagac gctgccagtg cgcgagtttt
     4321 tctgcacttt taaatcatag agggcaatcg cgccagtcat gacgccatca agaatacctt
     4381 ccgggatttc attgccttcg ctgtcccaaa tcacaggaat ggtcatcaaa tgacccacgt
     4441 tgcggataaa cagcaacgag cgtccgtgca gagaaatttc agagccatcg gcggcggtgt
     4501 actgacgatc gtcattcagt ttacgtacga tttgccgacc gtttttctcc attttctctt
     4561 gcagagtccc ctgcatcagg cccagcagat tgcggtacag caggatttta tcttccgcat
     4621 caaccgccgc gaccgaatct tcgcagtcga gaatggtact gatagcagct tcgacgataa
     4681 catcgttgat gtgcgccgga tcgtctttgc caatccgccc gttggcatca atttgcagtt
     4741 caatatgcag gccgttattt ttcagcaaaa tgcaggtcgg cgcagcggca tcgccacggt
     4801 aaccgacaaa ctgtgctgga gtacgtaacg tggtttcttt accatttttc aactggatgc
     4861 gtaattgttt atcgaccacc ttaaacgcca ccacatcctg atagctgccg ttttccagcg
     4921 gtagtgattc atcgaggaaa cgtcgaaccc aggcgataac ctgctcaccg cgctgcggat
     4981 cgtagccact gaccatcgcc ccttcctgcg ggatgatgtc gctgccgtat aacgcatcgt
     5041 acagtgagcc ccagcgagcg ttcgccgcgt tcagcgcgta gcgggcgttc attgccggaa
     5101 ccaccagctg tggccccgcc tgactggtga tttcgctgtc aatgcccgtg gtttccaccg
     5161 tcacgcgctc cggttgcggc accaggtagc ccagttcacg aaggaaagat ttataggccg
     5221 ctttatcttt taccggcccc ggattgctgc gatgccactc atcaagcgcc gcctgaatgc
     5281 gatcgcgttc tgccagcaac tgacgatttt ctggtgccag atcgtgaacg atctcatcaa
     5341 cattgtgcca gaatgcagca gcgtccagtt ccacgcccgg caaaacttct ccatccacaa
     5401 aacgtttaaa attggcgtca atgcgtaaac ggccctgggt tatggtttga ctcattgttt
     5461 ctctcctcgt tttcgcttat ttcgccaaca ccgctgctgc cactttcgcg acctgcgcat
     5521 cctgtgctcc ggttaaacca gaaacgccca cggcaccaat aatttgccca tcaacaacaa
     5581 ccggtacgcc gccttccagc gacgtcagta acggcgcagt cacgaacgcg gtacgtccgt
     5641 tgttcaccat ctcttcatag cccttagttt cacgacgccc cagcgcggcg gtacgcgctt
     5701 tctcctggga gatgtaagcc gcaatcggcg cgcaatcgtc catgcgactt aacgccagca
     5761 gatgaccgcc gtcatcggca acagcaatgg aaacagacca gttatttttc tgcgcctctt
     5821 cctgacctgc ggcaataatt gcactcgcca tttgctggct aagaatgact ttagttttca
     5881 tttttttatt ccttttcaag ggcttgttct acaatttcaa tccagtgacg cacagaggta
     5941 cgaccggcgc tcgccagatg cgtctggcaa ccaatgttgg cggtgacgat catttccggt
     6001 ttgccgcttt ccagcgcatt cattttgtta tcccgcagct ggcgtgccag atcgggatgc
     6061 gttaacgcat atgttcccgc tgaaccgcag cacagatggc tgtcgggaac gtccgttaag
     6121 gtaaatccaa gacgaagtaa cactttttcc acttcgccgt tcagcttttg cgcatgttgt
     6181 agggtacacg ggcagtggaa ggccagtttt ttatcgccgc gaattgccag tttttccagc
     6241 ggttcctcgc gcagaagttc gactaaatcg accgccagtt cactgacctg acgcgcttta
     6301 tcggcatata acgcatcgtt tttcagcatc tgcccatact ctttgacgaa cgcgccgcaa
     6361 ccgctggcgg tttgcaaaat tgcctcggca cctgcttcaa tcgcgggcca ccaggcatca
     6421 atattattgc gcgcccgtgc caaccctttc tcctgcgcat taagatgata gtccaccgcg
     6481 ccacaacagc ctgcttcgtt agctgacatg acgctgatcc ccagacgatc cagcactcgc
     6541 gcagttgccg cgttggtgtt gggcgaaagc gtaggctggg cgcagccttc cagcattaaa
     6601 acccgacgct tatggcgcag cggcggacgc ggtttagctt tcaccgtttc agcaggcagt
     6661 tttgctctga cctgttccgg taaaaacggt cgcagcacca gccctacctg cgtcagcgca
     6721 cggaagaccg ccggacgcgg cactacctgg cgcaatcctt cgcgcagcat tcgctccggc
     6781 agtgggcgtt tcactttctg ctcgacaata tcacgcccga tatccagcaa attgtgatag
     6841 cgcacaccag aaggacaggt ggtttcacaa ttgcggcaag tgaggcagcg atcgagatgc
     6901 tcctgtgttt taagcgtgac ttcgttgcct tccagcacct gtttaatcag atagatgcgc
     6961 ccgcgcggcc cgtccagttc atcgcccaga agctgatagg ttgggcaggt tgcggtacaa
     7021 aatccgcagt gaacacaggc gcacaggatg ctgtcggctt ccagcgcgcg ttctgccgca
     7081 tctcttcggt taatcgggtt tgcatagcct gctcctcaaa gttccgcgta catgcgaccg
     7141 gggttaaaca cgccgcaagg gtcgagctgc tgtttaagct gctggtgata gcggaataaa
     7201 ggagccgata gcggggcaaa gccaccatct ccggcactaa agcgggtcgc atgaccgcca
     7261 gcgttgcggg cgatgcgatg gatttgattg tcatcggctg tcgatttcag ccagcgtaac
     7321 gccccgcccc agtcgatcag ttgctcgccg ggtaaatcca tcatcggcgc atcgctgggt
     7381 aatgaaatgc gccataaggt acctggtaac gagaagaacg gcagttgttg ttcacgcaat
     7441 tgctgccaga actgacccgc aacctcttcg ccacccagca gttcacgcgc tgcttttacc
     7501 gatccttcgc cgccctcaag gcggatccac aacgcattgt cgaagtaaca taagccacta
     7561 atgggtaatg gctggagttg ccactcggcg atttcactca tggcttcttg caggctgatt
     7621 tcccgacgca ggctcagaga ggcacgcggt cgcggtaaca ctttcattga gatttcagtg
     7681 agcacgccaa gacaaccata gcttccggcc attaaccgtg agagatcgta tccggcaacg
     7741 tttttcatca cttcgccacc aaaacgcaga tgttttccag tgccggtaat gatgcgcgtg
     7801 ccgaggacaa aatcgcggac cgaaccgctc cacgggcgac gcggccccgc cagcccgcag
     7861 gcgaccatcc cgccccaggt ggcttcttca ccataatgcg gcggctcgca gggaagcatt
     7921 tgcccggcgc tctccagcgc cgcttcaatc gccaccagcg gcgttccggc acgagcggtt
     7981 atcaccagct cggtcgggtc gtaattaaca atgccgcgat gacaacgcac atccagcgtt
     8041 tgcccggtga ctgggcgacc taaaaaggct ttgctattgc tgccctgaat caccagcggc
     8101 gttttatcgc taattgcctg attcacctgc tccagcagcg cctgactgta atcacactcg
     8161 cgtagcatca aaagcgctcc agttcaggaa aaggtaaatg accgtgatgc acatgcatgg
     8221 caccaaattc agcacagcgg tgtagcgtgg gaatgttttt cccagggttc agcaaaccat
     8281 cggggtcaaa cgccgccttg accgcatgga aggtcgtgat ttcatcgctg ttgaactggg
     8341 cgcacatttg attgattttt tctcgcccga tgccatgttc cccactgatg ctgccgccaa
     8401 cttcaacgca gagttcgagg atcttcccgc ccagctcttc cgcgcgggca aattcaccag
     8461 gttcgttggc atcgaaaagg attaacgggt gcatgttgcc atctccggca tgaaagacgt
     8521 tggcaacacg taaatcatat tgctgcgata aacgggcaat gccttccagt acgccaggca
     8581 gggcgcgacg cgggatggtg ccatccatgc agtagtaatc cggggagata cgtcctaccg
     8641 ccgggaacgc atttttgcga ccggcccaga aacgtacgcg ctctgcttcg tcctgtgcca
     8701 aacggacgtc agtcgcgccc gctttcaaca agatgtagtt aacccgctcg cagtcttcct
     8761 gtacgtcaga ctccacgccg tccagttcgc ataacaaaat cgcttcggcg tcgacgggat
     8821 aaccggcatg aataaaatct tccgccgcgc ggatcgacag gttatccatc atctccagcc
     8881 cgccggggat aatgccattg gcgatgatgt caccaaccgc aagtccggct ttttctaccg
     8941 agtcaaagct ggctaacaga acccgcgcca cgggcggctt cggcagcagt tttaccgtca
     9001 cttcggtggt cacgccgagc ataccttccg atccggtgaa cagcgccagc aggtcaaaac
     9061 caggtgaatc cagcgcgtcc gatcccagcg tcagcgcctc gccgtccagc gtttgcactt
     9121 caattttcag caggttatgt acggtcagac catatttcag gcagtggacg ccaccggcat
     9181 tttccgccac attgccgcca attgaacagg cgatttgtga ggaaggatcc ggcgcgtaat
     9241 agagattatg cggcgcaacg gcctaggaga tcgccaggtt acatacgccc ggctgcacgc
     9301 gcgcgcggcg accaacgggg ttaatgtcga ggctctcttt aaagcgcgcc atcaccaaca
     9361 acacaccttt ttccagcggc agcgcgccac cagaaagccc ggtgcctgca ccacgggtca
     9421 ccaccggtac acgcaggcga tggcagacag ccagaatcgc tgtcacctgt tccatttgct
     9481 taggcagaac aaccagtaat ggacgcgtgc gatacgcgct caacccgtca cactcgtaag
     9541 gaatgatctc ctcatcggta tgcaggatct caagtccagg gacatgctca cgcagtgcca
     9601 tcagtaccga tgtgcggtcg acatcgggta aagcgccatc aagacgctct tcgtacaaga
     9661 tgctcatgag taggcttcgc tttgttgtgt tgtgtggcag ctgatttttg cgcgctgctt
     9721 ctgtgaacag ttattaagcg ggcttttcgt tttcgtctat ctctttagct accggtcaga
     9781 ccattttttt tccagctctg tgaccttgtc ttggttaact taatgttaaa ttgatgtaac
     9841 ataatcactt gcgtgatgtg cgtgttttgc gagttaagaa cagaaaaatt ggtcctacct
     9901 gtgcacgagg tccgggaatg aaagatgaac gtcgccctat ttgcgaagtg gttgcagaga
     9961 gtatcgaacg gttaattatc gacggcgtac tgaaggtcgg tcagccgctt ccctcggaac
    10021 gtcgactgtg tgaaaagctc ggcttctcac gctccgcact gcgtgaaggg ctgaccgtgc
    10081 tgcgcgggcg cgggattatt gaaacggcgc agggtcgcga ttctcatgtc gcacggctta
    10141 atcgggagca ggacaccagc ccgctgatcc atctgttcag tacgcagccg cgaacgctgt
    10201 acgatctgct cgacgttcgc gcattactgg agggcgaatc ggcaaggctg gcggcaacgc
    10261 tgggaacgca ggctgatttt gttgtgataa cccgctgtta tgaaaaaatg ctcgccgcca
    10321 gtgagaacca caaagagatt tcgctgatcg aacatgcgca gttggatcac gctttccatc
    10381 tcgccatttg tcaggcttct cacaatcagg tgctggtgtt tacgctgcaa tcattgaccg
    10441 atctgatgtt taattcagtg tttgccagcg taaataatct ctaccatcga ccacagcaaa
    10501 aaaagcagat cgatcgccag catgcgcgga tctacaacgc ggtgttgcag cggctgccgc
    10561 acgtcgctca gcgcgcagcg cgcgatcatg tacggaccgt gaaaaagaat ctccacgata
    10621 tcgagctgga aggccaccat ttgattcgct cggcggtgcc gctggagatg aacaaggtgg
    10681 gaatgtgagc agagttttac cctatcccca aggcgagggg accgcccctg cacttaacag
    10741 gatctggtgt taaatctgtt catctatacc ctctcccgaa tgaccgcccc tgcgctaaac
    10801 aggatctggt attaaatttg cttttcatac aatcggtaac gcttgtacgg ctccgcccca
    10861 atgcgttcca gcatgttatt catgcctgta ttggtttcga ggatccatga catctccagc
    10921 gcatcgatct tccggcgggc aaacggatcg cgtaaggctt caatcaataa cagcgcaatc
    10981 accgggccga tgcggctgaa ctgatactcg tcgcgcacgc ccatcagcgg tactcgcgca
    11041 gttcgcacac cgctgacttt caaacgccac agcaattttg cccagccgaa gggaaagagc
    11101 gatccgttca gatcggcaat cgcctcgttg atgttaggca agccacaata aacgcgcagg
    11161 gtgcagaatc aatctcagcg atgtagatca tatcgtccgg caccagatat ttaagttggt
    11221 cgcccatggt cgcgaattca tgttcggtaa acggcacaaa tccccagttg tgctgccagc
    11281 cagagttgaa aatctcacgc aggatctgca tctcttcggc aaaccgctga cgattgatgc
    11341 agcgaatggt cacctttttg cgcacctgat ccatcagttt ttttagcgcc ggagagaaag
    11401 tgagatcggt tcgctgcatc caccacgcca gtaaatcaat gcctttgtga taacccagtt
    11461 gttcaatatg cgcggcatac cacggtttgc cgtgtggcat catcgcacag ggtggtgtgt
    11521 caaaaccttc aatcagtaat ccgctttcct gattgatatt caggctgaaa ggcccgctga
    11581 tcttacttgc accttgtgac ttcaaccacg cttccgctgc gccaaacaac gcggcaaaat
    11641 cctgcggatc atcaatggcg tcaatcatgc cgaaatgacc ggtatcttca ccgtaacgct
    11701 cacggtgcaa ggtatctatt tgcgcggtaa tacgcccaac tatctgcccc gctttttttg
    11761 ccacccacgc ctgccagatg atatggtccg tccccggatt tttcgcagac aaatgctcgt
    11821 tgcgttcaat gaataaaggg ggtatccagt ttggatcgtc gggataaagt gatgacggaa
    11881 aagcgataaa tgccttaagg tcatttttat taattacttt ttcaatttta atcatcagca
    11941 gatcgcattc catatctctg tggataacaa ataattaatg gtgaaaaaca ggttgcaaca
    12001 gcaacgagcg gcatcttaat aatcaactca atgaatacaa acgacgaata aatagtatta
    12061 attaatgatt atgataaggt cagaggctac acagaaacgc ctcaatgcct tttgtcaatt
    12121 taaggcgacc ggacaaggct tcttcttaat atattcataa attaaatatc attcataata
    12181 aaaacaaaaa acttaaactc attatttaaa tcaatgttca ttttcacacc gatgaaaata
    12241 ctatctcatt tatcttttat gcctatataa tccaccgcat tattgtcatt ttatttttga
    12301 tatccaattt tttcgtggtg tatatgtcta ataaaatctt tacgcattcc ctacctatgc
    12361 gctatgccga ttttccaacg ctggttgatg ctttggacta cgccgctctg agtagagccg
    12421 gaatgaattt ttatgacaga cgttgccaac ttgaagatca actggaatat cagacattaa
    12481 aaacgcgtgc cgaagctggt gcgaagcggt tgttatcgct gaacctgaaa aaaggcgaac
    12541 gcgtggcgct gattgccgaa acgagtagcg ggtttgtaga ggcttttttt gcctgtcagt
    12601 atgctggctt agtcgccgtc ccgttggcga ttccaatggg cgtcggtcag cgggattcct
    12661 ggagcgccaa actccagggt ttactggcaa gttgccagcc cgcagccatt atcactggtg
    12721 atgagtggtt gccactggtc aatgccgcga cgcataacaa ccccgaatta catgttttaa
    12781 gccacgcttg gtttaaggca ttaccggaag ccgatgttgc gctccagcgt ccagttccga
    12841 acgatatcgc ctacctccag tacacctccg gcagcacccg ttttccccgt ggcgtcatta
    12901 tcacccatcg cgaagtgatg gctaatctac gtgctataag ccacgatggc attaaattac
    12961 gccctagcga ccgctgcgtc tcctggcagc ctttctacca tgatatggga ctggtcggct
    13021 ttctcctgcc ccccgtcgcc acgcaacttt cagtagatta tttgcgcact caggattttg
    13081 ccatgcgtcc tttgcaatgg cttaaattga tcagtaaaaa tcgcggcacc gtttccgttg
    13141 cgccgccgtt tggctatgaa ttgtgccaac gccgcgtgaa tgaaaaagat ctcgctgaac
    13201 tggatctttc ctgctggcgc gtcgctggta ttggtgctga gccgatctcc gcagaacaac
    13261 tccatcaatt cgctgaatgt ttccgtcagg ttaactttga caataaaact ttcatgccgt
    13321 gctacggact ggcagaaaat gcgctggctg tcagcttctc tgatgaagcc tccggggttg
    13381 tggttaacga agtggatcgc gacatcctcg aatatcaggg taaagccgtc gcgccgggtg
    13441 cagagacacg cgccgtatcg actttcgtca actgcggcaa agcgttgccg gaacatggta
    13501 ttgaaatccg caatgaagca ggtatgccgg tcgcggaacg tgtggtaggc catatttgca
    13561 tctccggtcc cagtctgatg agcggttact ttggcgacca ggtttcgcaa gacgagattg
    13621 ccgcgacggg ctggttagac accggcgacc tcggttatct gctggacggt tatctgtatg
    13681 tcaccggacg cattaaagat ctgattatta ttcgtggccg taatatctgg ccgcaggata
    13741 ttgaatatat agcggaacag gaaccggaaa ttcattctgg cgatgcgatt gcttttgtta
    13801 ccgcccagga aaaaatcatt ttgcagatcc agtgtcggat cagcgacgaa gaacgtcgcg
    13861 ggcagcttat ccacgcgctg gcggcacgga tccaaagcga atttggcgtg accgcggcta
    13921 tcgatctgtt gccgccccac agtattcccc gaacgtcctc cggcaagcct gcccgtgcgg
    13981 aagcgaaaaa acgttatcag aaggcttatg ctgccagtct taatgtgcag gaatccctgg
    14041 cataaatcaa actgtcgcgg tgacgggcgc taccgggttt atcggtaaat atattatcga
    14101 taacctgctc gcccgcggct ttcacgttcg cgcattgacg cgtactgccc gcgctcacgt
    14161 caatgataat ctcacctggg tgcgcggttc gctggaagat actcattcac ttagcgagct
    14221 ggttgccgga gccagcgtag tggtccattg cgccgggcaa gtgcgcgggc acaaagaaga
    14281 gattttcacc cgctgtaacg ttgacggcag cctgcgcctg atgcaagcag caaaagagag
    14341 cggcttttgc caacgttttc tgtttatctc ttcgctggcg gcgcgccatc ccgagctctc
    14401 ctggtatgca aaatccaaat acgtcgccga gcaacgactg gcagcaatgg cggacgaaat
    14461 tacgctgggc gtttttcgcc cgacagccgt gtatggtccc ggcgataaag agttaaaacc
    14521 gctgtttgac tggatgctac gcggcctgct gccacgactt ggtgcgccag atacacaact
    14581 ctcttttctt cacgtcaccg attttgcgca agcagtgggt cagtggttaa gcgccgaaac
    14641 tgtacagacg caaacctatg aattatgcga tggcgtcgcc ggtggctatg actggcaacg
    14701 cgtacgacag cttgccgcca aagcccgttg tggttccgtg cgaatggttg acattcctct
    14761 gccggtactc acaggccttg cggatatcag taccgcgttg agtcgcctgg caggtaaaga
    14821 acctatgctg acccgctcga aaattcgtga attaacccac gccgactggt cggcaagtaa
    14881 taaccgtatt tctgaagata tcaattggtt tcccgggatt agcctggaac acgcattacg
    14941 caacgggcta ttttgattgt cttgttttaa aggtgtgtta acgtttctat ggtaaatcgt
    15001 gaaatagtaa tggattacat cctttcctgt ttacaggatt tggtcgaaaa cggtgtggaa
    15061 attaagcccg acagcgatct cgtcaacgat cttggcctgg agtcgatcaa agtgatggat
    15121 ctcttaatga tgctggaaga cagattcgat atttccattc ctattaatat tctgctggat
    15181 gtcaaaaccc ctgcgcaatt aatggaaact ctacttccat ggctggagaa taaataatgg
    15241 ggctatacga taaatatgcg cgccttgctg gcgagcgtct gcaatttagt gacaacgggt
    15301 taacaccgtt tggcacctgt attgatgaag tgtattccgc caccgaaggc cggatcggca
    15361 ataaaaaagt gattctggcc gggaccaata attatctcgg tttaaccttt aatcatgatg
    15421 ccatcagcga aggtcaggcc gcgttagcgg cccaaggcac cggcaccacc ggttctcgta
    15481 tggcaaacgg cagttatgcg ccgcacctgg cgttagagaa agagattgcc gagtttttca
    15541 atctccctac cgctatcgtt tttcaaccgg ttataccgct aatttaggcg tcatcagcgc
    15601 acttgccgac cataacgccg tggtgttact ggatgccgac agccacgcca gtatttatga
    15661 cgcctgttct ctcggtggcg cggaaattat tcgtttccgc cataacgatg ctaaagatct
    15721 ggaacggcgc atggttcgcc tcggtgaacg ggcgaaagag gcgattatca tcgttgaggg
    15781 catctacagc atgctcggtg acgttgcgcc gctggcggaa attgttgata tcaaacgtcg
    15841 tctcggtggt tatttgattg tcgatgaagc gcattcgttt ggcgtgttag gcgcaacggg
    15901 gcgcggactg gctgaagctg tcggcgttga agatgatgtg gatattatcg ttggtacttt
    15961 cagtaaaagt ctggcctcga ttggcggttt tgctgtcggt tcggaagcga tggaagtgct
    16021 gcgctacggc agccgcccgt atatttttac cgcctctcct tctccgtcct gcattgcgac
    16081 cgtgcgctcg tcgttaagaa ctattgccac acaaccagag ctgcggcaaa agctgatgga
    16141 taacgccaac catctgtatg acggtctgca aaaactgggt tatgagttaa gttcacatat
    16201 tagcccggta gtgccagtaa taatcggttc gaaagaggaa ggtttacgca tctggcgcga
    16261 gcttatctcg ccgggcgtgt atgtgaatct tatcctgccg cctgccgcgc ctgcaggcat
    16321 cacgctgctg cgctgtagcg taaatgctgc ccacagtcgt gagcaaatcg atgccatcat
    16381 tcaggcattt gctactctga agcagtaatg tgagcccgcc gtggtgtttg cgccacggcg
    16441 ggtttaatgt tgtctgtcat accaatacac caggctcaac gccaccaaca caaacgctac
    16501 cggtaaaccg agcgcggtaa caggtgcgct taacgcaaat agcaatccca gattaaccat
    16561 gatttgatag ctgatccagg tgagtaagcc cacgatgacg cctacagcga gacggctacc
    16621 catcccaggc gaacgcgggg cactaaaggt aaacggcaca gccagcaaaa tcatcgccag
    16681 ggtcaggatg ggttgcccca gtttttccca cagtgcaagg cggtattcgc tgctgggttg
    16741 cccggtttcc tgcaaataat ggatgtaatg attaagctga cggacagaaa aagtattccc
    16801 cggcattgat aactcttcaa gattcatact ggtgaaggcc gattgccagg caagattatc
    16861 taatgtttcc agcgtttctt tgccattcag ccatttttta tgattcacac catgcaggac
    16921 ccacgttttg tcgtcttcaa tggtggcact gcgtgcataa atgtaggatt caagcgagag
    16981 atcgtcgcga taatgaaata tctccacgcc caccggctgg ccctgctcgt tcagcgattt
    17041 caccgtcaca aattcattac cacgcctggc ccacagcata ttgccggtaa tgtcatcgtc
    17101 ctcccccaac gcggtggcgg tcgatttgat ttgcaacgcc tgttgctgca atggcgacgc
    17161 cacccactca tctatcgcgc ctaacgaaac agtccacaat atcccggcaa ccagcgccac
    17221 cagtgcaata cggaagatag aaaaccccgt gctgcgaatg gcggtaagct cactgttttt
    17281 cgataactgc cccagtccaa caataccacc caataacgcg ataaacggcg aaagttcgac
    17341 cagtgtgcgc ggtaaggtca ttagcaccac cagcaccgcc tgagtccagc gataaccgcc
    17401 cgggctgaca ccatccagtt cgttaatcag gttgaaggtg gtaaaaagcg gcagcaatag
    17461 ccctgcagcg gcggcaaaac cgagaaagag atgacggatt aaatagcgac tgaaaacatt
    17521 catcgggaaa atttctgcag caaagagaag tcacgtgcga ccagtatcag caggcccatt
    17581 agcatgagtc cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc gttagccacc
    17641 agtgtacggc agacgtcgcc gccataaaaa atggcaataa acagcaacgt cagtggcaat
    17701 aacgttgaaa atcgcccttg ccgcggtttt acccgactta atgaaatggc cagcagcgcc
    17761 atcaacaatg cggtcaggcc acggctttgt cgccattgca gttcggcatg atcggcagga
    17821 aaggcggagc gcgccagctc cgtgaccgat ttcgctttgc gtttgacgtt cgggctttga
    17881 tccagcggat tcagatgtaa ttgcagatta cggtagatct gttcgttgtc gtcacgcccc
    17941 tgatgatcga gaagataggc agtgccgttg tgcaacataa cggtcggttt ctcgggtgat
    18001 gggtcaacca catcaaccga accggcgcgg aaaatacggg ttcgattggc agtagaagta
    18061 taaatcagcg cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga aaggatcatt
    18121 cgtccgttat cgttagtgtt aaatttcttt gcccgcaact ggcgaacatc cagctccgac
    18181 tgcgactgct gctccagttg ataaatctgc gcataagccc acggacgacc atacatcgac
    18241 agaagggtga caaaaatact tagcggtatc gccagataaa gaacggcttt gtacaagcgt
    18301 cccggactgc cccccgccgc agagatagcg gtaatttccg agtcggtata catctgccct
    18361 agcgtcacgc caactgacac atacagacca acaggtaaca acatctccag tgcaatcagt
    18421 actttgtaga aaacgatatc cagcacaaca tcaagagcta atgtcccatt tgccgcttcg
    18481 gtcagataac gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc gacgataatc
    18541 agcaccagac ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat ctcactaacg
    18601 ccctcaatcc tttgagtttt ttattcattg atcagtttct gcgccaacgc aatgacatga
    18661 tctacctctt ccggcgtcag gctgccgtcc tttgcccact gaatttgccc ggctttattc
    18721 agcaccagaa tggttgaact ctgctccggc aagcgccagg caacgcgtcc ctgtccattg
    18781 ccgtcaataa cgaattgcgc ccacggatag cggcgtttat ttttttcgat tttgccaaca
    18841 acaaaatagc cagtaccaaa gatggcatca tcggtgttca caatggtggt gggctgaaaa
    18901 cgatcctgcg gaaagtttgc cgcttcaacc gctttaatta acagtgagtt ctttttcttc
    18961 gccgacttac gcccggcgat atactgaata atgcggactt ttcccgctaa ttgtgtgctg
    19021 ttccactttt ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc gccttcctgg
    19081 atataaacag gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac acccggggct
    19141 aagagcatga taatcaacgc aataatcaaa cgagatgaca tcattgctcc ggctatagcg
    19201 gaaaacgcga aatcagcagc tctaagcgct gacaaatatc gctatccagc gccaacggtg
    19261 cataatacga ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt ttatctccca
    19321 tcaggccatt aatggcggcg aggaaacggg aaatctgtaa cgcctcttta ttttcaggat
    19381 caagcgatct taaggtgtcg taataagaaa cctgctggct gatgattttt cgcacatcat
    19441 gaaattccct gccggttagt cgcggtttgg caataatctc tttcagggta tgcatttgct
    19501 tttgccgata ggcaataaaa ctgcttacgc tttctaactg gctatggcgt aatgaatagt
    19561 tcaccaacga ccagaccgga gagcttaaat aaatccgtaa caggttgccg taatacgaca
    19621 caatattttt cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa acggtcgttt
    19681 tgccgaataa aaatccggcc tgatgcttct ttaaatacag ccgttgcgca aaacgcaggt
    19741 gcttgtagcg cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc agctcatcgc
    19801 ctttggcttg tttgcgaact aaacgcagta attcctgacg atcaacgcca ttgagccaga
    19861 attgcaggca taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt agtgcaatag
    19921 tcggcggcaa actaacctgt gcatcaacga catcatcttc ttcaatcgca acaaatagac
    19981 gctcgctcgc ctcaagcggg aaatattttc tcatcatcag gatttctctc cggctgcgtt
    20041 ataaagcaag gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg agagcgccac
    20101 gtccagacat tgcccaccga agtaagcaaa cgctgctccc agcacaccaa acagatgaca
    20161 gaacatgaac agcaatacga tgtagccgat tgaggcgatg gtttgcgcca cgagaaaagc
    20221 gcgctgtttt cccgccatca atagcaatga ctcctgcggg aagcccagca tcgaaatcac
    20281 aattgcgccc agcatcacct gaatcaggtc atacgcttcg agatacttaa cgccaaacac
    20341 cagcgaaatg agcggcttgc cgacgatcaa caccgcgaga gccaccagaa taccgattcc
    20401 gcccgccagt aaaccggact tcacacctaa caaccacggt ctggtggtgc gcggatctaa
    20461 acgcatcacc tctgggtaaa aacttttacc cagcaaaccc gctggcgttc cggcggcgtc
    20521 gaagaatgtc atggcgattt taaataaccc ggcggcagcg ggtcctaaca cgatccccac
    20581 caacactgtg ctgcacgagt tacgcgccga ccagatggag tgggcaatgt tggttgacca
    20641 gacaaaactc cacgcgcctt taatgtgtcg ggcagactca aacagattca atttgaaggc
    20701 gttatggata tttctacggc gtaattcgcg cgcggcaaac caccagtaca tggtgccgcc
    20761 aaccagattc gacacgtacc aggcaataac aaaacccgca aaaccaaagt caaaatacca
    20821 ggctgcgacg ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta cagcaatcaa
    20881 atcaaagcga tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca ttgaaggaat
    20941 gagcgtgcaa tacagcgccg ccagccaaaa gctttggtca tccagaccta atgaatggga
    21001 aaggaatggc agtaaggcaa tgccgccgac aatcgccacc gcgccgctga cgatatccag
    21061 cgagaaggaa aatgagacga cattgcgaaa ttgctgcgga ttattgttgg tcaaggctgg
    21121 cgttccgtac tgaaccacca gctgccatgt ctgaaactta ataaaatcgc tgatcgactt
    21181 ggcgtacgat tgcacaatca ccagtacacc aaacatggcg ggcgtcatcc ctttaccggc
    21241 acacgagagc gccaacagcc ccagcaaggc gctctcgaca ttactggagc ctaaccaggc
    21301 gctattgcga ataatggtgc gaaacgcgcc atctgcaaac caatgtttga tgttaaaacc
    21361 cgccaattca gcctgacctt atcgttgata gtaaaaaata tcccgccagc cttaagttaa
    21421 acttcggcgg tcagaaacga tggcaaccag agaaaccgcc ttctgtacct gttccagcac
    21481 ttcgctgtag ggcgctctgg aatcaatctc aagaattttt gtgccgttat agccaatcgt
    21541 cgacatgacg ccgattttgt cctgcagctc ggcatactca tggtcaggct tgcgggagat
    21601 ggcagtatca atatcaatcc ccaaacgaat gattaattca ggacggtatt gcgccatttt
    21661 ttggtataaa ctccgttcgc gctgcgccag aaacatgctg attttcccgg tcgcacgttc
    21721 gacgccgatc cccggtccgt cataataaaa gcccgaaatt tcagcctgcg ggaagcgatc
    21781 gctgaccacc agaacgccac tttgcgccag tcgctgaacc ttgcgtagat tcgccattct
    21841 tcgcagcgag aagcagtaca taatcaccgc cgcccatagc gccggagatt tggttttcat
    21901 gctttgggtt ttcgaggatt tggccgccaa tcgccgttcc agccagacgc caaccaacgg
    21961 caatcgtttg attttgtcgc cgtcttcgcc ggagagcagc cccagatagc ggcgctcggt
    22021 ttgccagtgt tgttgcagcg atttcaccag gtcggtggtc agcgtggatt taccggtgcc
    22081 atcacacccg accaccgcaa tcagccccgg aatgtagttg ggctgcggcg cggttgtact
    22141 attaacagtt tgtgtttgta gtgcatccat tcgttaaatc atcccctatc cattttggtg
    22201 atcaggaaag cgcggcgtga attgcgcgta gtgacgcttg cataatttca tcggctggtt
    22261 gccgaccatc cagttcaagg atcttggcgc cattgaatgt cagttgcggc gtaacggcga
    22321 ttttttcctg taacgctgcc agttggtggt caggtttacg cgcaaacgcg gtttgttcat
    22381 caatgccaag acgaatcaac aatacgggta aataagacgc catccattgg tacagcttca
    22441 actcgcgctg ccttaacatt tttatccaac cgttaccgcc cgtggttttt gccaattgcg
    22501 ggccatcaaa gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg atgagcagat
    22561 agccttgctg gcttttacac aacattttgc gaaacttgta cgcccgccag caggaaagca
    22621 gatagataac cagcgcagta atattgccag gcggagttaa gggcttttcg tgtacatgtg
    22681 ccgctttact tcgcagataa cacccacaag gtgcgccaat aacagggagc tgtgaaatcc
    22741 attcgccaat tcgcccggac gattgcccga gataaatgtg ttctgttggc attcttgctg
    22801 ccagttcatt taccaggctt gccgtgaggg tcgatttacc tgaaccatca catcctacaa
    22861 tggcaataac acgcactggc gtggaattaa ttattgacat attacgttga ttcacgaaaa
    22921 acccggcagt aaataatgta ttgaatatta cgtggtccgt gctcagaata tccgttcaac
    22981 cttattgtga caatggctaa ttgtgacatt gacctgaatt acgctttcat aaaaacatat
    23041 taaccaaata aatattttta atggatattt aaattaaagg atatattcat gcagtcaata
    23101 acacctccat taattgccgt tattggtagc gatggttcag gcaagtcaac ggtgtgtgaa
    23161 catcttatta ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacag
    23221 gccggaaatg tcggtcgtgc agtgacaaaa ttacctttga tgggaaaatc cttacataaa
    23281 acaattgaac gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt tccggcgctg
    23341 gtaattacag cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct tgcctgtcgt
    23401 cgtcgcgggt taattgttct aaccgaccgt tatcctcagg accaaattcc tggcgcttac
    23461 gatggtacgg tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg gctggcaagc
    23521 caggaacgta aagcgtttca ctggatggcg agccataagc ctgatctggt catcaaactc
    23581 aatgttgacc ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga atcgctggcg
    23641 aggaagattg ccataacgcc acagttaacc tttggtggtg cacaactggt tgatatcgat
    23701 gccaatcagc cactggaaca ggtgttggtt gatgcagaaa aagcgattac ggattttatg
    23761 accgcgcgtg gttatcacta gtcaaaaatg gaaatgcccg atcgccagga ccgggcattt
    23821 tcaggaaggt taaatcaact gcaatgctat ccagtacagc ccaccagaaa gaaaaattgc
    23881 cgccggtaaa gtaaacaccc acgccatcag gatgctggtt accgttttac gctgtaaccc
    23941 accgccgtcc accaccatcg tccctgctac cgcagacgag aggacgtgtg ttgtggagac
    24001 aggcatccca atataactgg caaggccgat agacactgcc gccgtcattt gtgccgccat
    24061 gccttgcgca tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg
    24121 acgccagcca atcatggtgc caatgccgag cgccagtgct accgccatga tgatccacac
    24181 cggagcgtac tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc
    24241 ttctttactg acgcctggca gtttcgccag cttcgcggag gtatcggaga tgcacagcat
    24301 aatgcggcgc agctggctgc gctgactcac gcttaacggc tcgtaacttt ccatattgcc
    24361 tggcagcatc gttttaacgc gcgcaatcgc atcaaaggta tttgccggat gacagtgaaa
    24421 ctctgttact tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca tcgcaatcaa
    24481 cttctgcggc agttcaggat gctgttgcag gtagtgttcg aagttggtaa cggcatcgcg
    24541 ggtacgggta atttcatagc cggacgcatt catattgacg acgaagccag caggggcaat
    24601 ccccaccagt accagcatta ccaggccgat ccctttctgc ccgtcgttcg cgccgtgcga
    24661 aaacgccacg cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg gcggtttacg
    24721 tttgcccttt ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt
    24781 cccactccag aaacggcgca gcaggaaaat cagaccaccc gcaatgacca ggccgacgat
    24841 aggggaaaca atcagcgagg agaaaatttt ggttacttca cgcaggttta acgcatccat
    24901 caccgatgag ccggttaaca gcgcgttggt taaaccgatg ccgataatcg caccaatcaa
    24961 ggtgtgcgaa ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc agataatcgc
    25021 cgccagcagc atggaaaaga ccatcgccaa accgtgagtt gaccccatat tcagcaacaa
    25081 atcgattggt aacatatgga caatggcata ggcaacgcta agtccgccca ataacacgcc
    25141 aaaaaagtta aaaaatgccg ccatcaccac agcaagttgt ggctgcatgg cacgagtata
    25201 aataacggtt gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa
    25261 cacaaatgcc agagcaagta ataacaaaag ccccgtatat atatcaaggc caacaaataa
    25321 atttagcata tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact
    25381 ttaacggcat gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg ttatttcttg
    25441 tgtatcagac aataagcatt cattcaaaac ctttagaaat aatcaaaaca caggtattcc
    25501 actggttaat atattcacat atgaaatgaa taaatattgg aatatataaa tattgaatat
    25561 tttgattaat ccccgtacta attattcttc ataatcagta cggggttgct acaacatgta
    25621 ttactttttc accacaatta acggttcaat atcactctct tttttgatga ccagtgattc
    25681 atcaccgcgc aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac atacctgaat
    25741 gtatttaccg tccactttcg gcaaacacca cagttgctga tagatgtttt tctgctcggc
    25801 aaatttaccg ctggttttgt ccagcacctc ttcatgatgg ctgaccaggt cgatattgct
    25861 accacagcga ccggcgatcg gtttcactgc gtaacctgtt ttcaccaatt catcattaac
    25921 agtgaaatcg gtatccagca ggtaacgatg gtgcgggaac agcgaccaga ggatcggcag
    25981 aatcgctttg ttgccgggga tcaccgtcca cagcggctca aagaccagca cttccgggcg
    26041 cagcaatacg tcgataagac gcacttcgtt ttgcggatga ccggtacgga ttggcaccgc
    26101 agcaaactca cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc acgcccaggt
    26161 tttccacacg cagttaacca gtcgcccttc cccatcaatc agttgcccgg cagcatccca
    26221 gcccagttca tccagcccgc gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg
    26281 ctccataaac tgcgcgtgat agttttcctc gatatctttg tcctgcatga tatggacaaa
    26341 cggacgtgca cgactgtgtt tccaggcacc agccagttcg ttaatcagcc cttccgccgg
    26401 attgaagccg ttgcctttat agccctgctc cgcccaacgt tcgaggatca agcccgcttc
    26461 ggtatgacag gaggcggagt cggcgttgta ttcgtaaacc ttcaggccac gctcatccat
    26521 gcagaaatcc atacgaccag tgatcatatg gtgacggcga cgctgccagg agagacgcaa
    26581 acgtggccag aggattttcg gaatgtcgaa cagcgccagc aggttgtcat ctttcagcac
    26641 cttgtcggtt gcgtgaagat acatcagatg cagctcgttg gtggctttaa ttagctcctg
    26701 ctcggcactc tcggtaatgg tgtagtaatg atacggatcc tgattgatca cctgaccgtt
    26761 ggcctgcaca taggcgtttt gtagcggatc tttttcatcc agccatttac cgtcaaactg
    26821 gcctttgttt tccagacgcg ctccactgat tttcagcagc tcgcctgcaa tttccggctg
    26881 cggtaagctg tattcagtat cttccgtctg gatcatccag cccagaatgg tggtgtcatc
    26941 aaaagtgtct ttaagggtat agcagccgtt ttcgaccacc atctccagct cgcgcgtcca
    27001 ctgttgccct tgcggcaacg gggaatgaat cacgttctgt tccgcaatac ggactttgtt
    27061 gccatgcaat tgggtaatga tggcgacatg gccagtgtct ttaaattcac cgcctttatc
    27121 ccagataaga agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa atgcctgcaa
    27181 tggcaggatg ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa tctcccacgc
    27241 catacccacg tcagtaaaga ccacaccgta attcagaaag agaaaacggc gagcaaattc
    27301 aacgcattgc cacttgtggc ccatatattc gtcgtcgata tagctacgga atacggcgtc
    27361 atcttcgtat tcctgcggat cgagagaact gtaatctgaa gagtagattg ctaccccacc
    27421 tggggcgtag cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat
    27481 cactttacct ttaacaatac aacctaagca gttggcgtaa ttgttgtgct ctgattacct
    27541 gctcagcgac attaaccgga cagaggtcag actaatcata cacctcatcg cagcggctgg
    27601 cgcaaagggt taaaaaattc acattctgta cagcaagtga cctgctacac tgcttcaaca
    27661 ctaccactca gaaggcaact cactatgaca gacaatactt atcagcccgc gaaagtctgg
    27721 acgtgggata aatccgctgg cggcgcgttc gccaatatca atcgcccgtt ttctggtccg
    27781 acgcatgaaa aaacgctgcc cgttggcaaa catccattgc aactttattc gctgggaacg
    27841 ccgaacggtc agaaagtaac gattatgctt gaggagctac tggcgctggg cgttactggt
    27901 gcagagtacg acgcctggct gattcgtatt ggcgatggcg atcaattctc cagcggcttt
    27961 gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg atcatacgca taatccgccg
    28021 atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt tggctacttc
    28081 ctgccgcagg atctggcaaa acggactgaa acgttgaact ggctgttctg gttacagggt
    28141 gccgcgccgt tcctcggcgg tggttttggt cacttttacc attacgcgcc ggtgaaaatt
    28201 gagtacgcca tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt gctggataag
    28261 caactggcgc agcataagtt tgttgcgggc gatgagtaca ccattgcgga tatggcgatt
    28321 tggccgtggt ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga gtttctggat
    28381 gcgggaagtt ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg tccggcagtg
    28441 aaacgtgggc gtattgttaa ccgcaccaac gggccgctga acgagcagtt gcatgagctc
    28501 catgacgcca gtgattttga gacaaataca gaagataagc gtcaggggta agggttggtg
    28561 ttcgtcgcag caagccatcc aggccggata aggcgttcac gccgcatccg gcaatcgtgc
    28621 acaatgcctg atgcgacgct gccgcgtctt atcaggccta caaaagctaa tcatcacgcg
    28681 ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg tggctttggc ttcatcttcg
    28741 tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc actggcccag cagatcggca
    28801 gggttacctt cgcaaatcag ggcgatattc acgtcgcgct tgataccaca tacttcaacc
    28861 tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct ggcctggaac gccaatacac
    28921 attgttaact ccggttattt cgattgccgg acaaacgcga aaaagaacct ttagcgggtg
    28981 ttccctctca ccctaaccct ctccccggag gggcgagggg accgtccgtg cgctttttag
    29041 gcgttgtcct cagtcttatt caacttcaat acttttgacg atcagcgaat cgccggtatc
    29101 gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaatctccac
    29161 cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca
    29221 cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc
    29281 aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg
    29341 ctccgcctgc cgttggataa tttcaacggc gctctggcaa agcgacaact catgcatttt
    29401 cgctcctcct gcggccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattgg
    29461 tgactggcaa cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccttcttcaa
    29521 tcgacatgct gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc gaaacaccgt
    29581 ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca attttttcac
    29641 tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc
    29701 acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca
    29761 ccggcattga aggatgcaga aaagagagat cgtgcatcga acgccgggca atttcttcaa
    29821 acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga
    29881 atcgcctccc gtgggatggc ttccacgcct gattcgcgca gcgcagccag aacctgctca
    29941 agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat
    30001 tccgggatca cgccgaccag ggtcagcttt ttcggaaact caccggtgaa gcgcagggcc
    30061 gacaatacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga
    30121 acttcttcat cccgcaggat catcatcgtt cccggagtgt tctttttcga cacaatggca
    30181 tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg
    30241 ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca
    30301 atccgcacgc cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata
    30361 cgcattacag aaccttcact gaaaccactt cgttgccgtc ggcatccact acgtgtaccg
    30421 cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg
    30481 gatcggcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt
    30541 tacgcggacc agagttccag gttgatggaa caaccgcctg gtagttgctg atgataccgt
    30601 ctttaataac catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt
    30661 tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg cctttgccga
    30721 tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca cagcagtgaa
    30781 cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc agcgtgttgc
    30841 cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta gattcgcgac
    30901 ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt ttgccgtaga
    30961 aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa
    31021 tggtggtacc ttcccacggc gcctgtggcg cttcgtcttt ataccaggag tgcttcgcgc
    31081 tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg
    31141 acagatccgc attctcaatg tagcctcccg ggaacaggaa gctgccgttt ttgctgtcgg
    31201 tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg
    31261 ggtagaacgc ggcgataacc gcggtatcaa ccttataaac ctgctcaaca aagtcgctca
    31321 gtttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcaacacg cccaacccgt
    31381 cgaggttgat tgggttcgcg acaccaccca ctgccaggtt ctgaatgtgc ggcgttttac
    31441 cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc tgcaggtagt
    31501 gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc
    31561 cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt
    31621 tagtgaactc ttccggactg ttcagatgcc aggtcgaaac gcctttcagc atttcggagg
    31681 ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct
    31741 gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt
    31801 attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca
    31861 gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg
    31921 gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt
    31981 tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta
    32041 ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt
    32101 ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc
    32161 aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc
    32221 gccgcccggg ttgaatgcca ccaacgaata ggtcagacgc caggttgcac aacctaacag
    32281 tgcgctcagt gctgacagga acagcatgcg ggaatcatta cgcagcttcg ccacacgcag
    32341 aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc
    32401 accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcccgat
    32461 gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaacaggc tcttttcatc
    32521 cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga caatcgagaa
    32581 gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccagag
    32641 cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt
    32701 cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac gttgtagcga
    32761 caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca tcacgccgat
    32821 gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg
    32881 aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc
    32941 cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc
    33001 acggttaaag acgtataccg cccacgccag cgcccagccg ccacaggcaa agccggtgcc
    33061 aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac
    33121 agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg
    33181 tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact
    33241 catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtta
    33301 agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt
    33361 cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca
    33421 cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat
    33481 aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt
    33541 ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac
    33601 gcgtaccgaa aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt
    33661 tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg
    33721 ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcaac
    33781 cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag
    33841 agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt
    33901 acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacctgaa
    33961 tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg
    34021 ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc
    34081 acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag
    34141 ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa
    34201 taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg
    34261 acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa ccacaccgcc
    34321 gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt
    34381 ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc
    34441 gatacctttt tcgttacagc catagcacgg gtgaccaatc gccaccggcc acacaccgcc
    34501 aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag
    34561 gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg caaaacgacc
    34621 agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt
    34681 cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt
    34741 cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg
    34801 cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc
    34861 ccacgcggag caggaaccaa tagcgataat ggctgctgag ccttccgccg ctttgcggat
    34921 gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt ttaatgggat
    34981 ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt tatgtttgtt
    35041 ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatattcca gagagatagt
    35101 ctccagcacg aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc
    35161 ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc
    35221 ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag
    35281 cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat
    35341 ttatagttat tccgttgcga ggacctggca tatattttgc ctcaatcgca aaatcaataa
    35401 tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg
    35461 cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt
    35521 atgtttgatc aattttcgag gcaataaccg gccataaaaa agcgcaacgt agaacaggaa
    35581 ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga
    35641 atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat ccggtgtgag
    35701 tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg
    35761 tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt
    35821 cacctcattc gttatatatc gccatatata gcgatatttt aggcaaagaa cgacttttgc
    35881 gcacgctatt ttttcatact tgtttaagac gtaatcccgt aagagataaa cctatcagga
    35941 gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg cagatttgtc
    36001 ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga tataagcctc
    36061 ataaacctta ttattgtctt tcaacgccgc ctcgtaagga ggccagccct cgttgattcg
    36121 ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt
    36181 gggtgcctga cgaccataaa acggcaccgc gcaggccagt tccggatacg cgacagccgc
    36241 cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttac ctgtcgcttg
    36301 cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc
    36361 aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc caacggaact
    36421 taatccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc
    36481 gatatacgga ttcagtccac gattctcatg caccaccccc acggctggcg ttttgccgct
    36541 catttttgcg ggtttcacca gataaccccg tacctcgccg tgaccatttg gcgaaggata
    36601 cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata
    36661 atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg catacttcgc
    36721 cgcaaggttg aggaactcac gtttcgaaat tttcccgtga acgtaatagt cgtagtaatc
    36781 caacaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcat tcctcaactg
    36841 gtgttttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc actctgtgag
    36901 ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa
    36961 cggaggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcag
    37021 taccgctatt gcggaaaaag tggtttacgc ctgcccgcat tatcgctcgg tttatggcac
    37081 aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa agcgtttgat
    37141 ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa
    37201 gagaactttg gtcgcctgct gcgcgaggat tttgctgctt atcgcgatga actgattatc
    37261 tctaccaagg ctggctacga tatgtggccc ggcccgtacg gctctggcgg ttcacgtaaa
    37321 tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta tgtcgatatc
    37381 ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct
    37441 catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc gccagagcgg
    37501 acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat tcatcaacct
    37561 tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat
    37621 aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac cggaaaatat
    37681 ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt tcgtggtctg
    37741 acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa tgaaatggca
    37801 cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgatcgc
    37861 gtgacgtcgg tattgattgg tgccagccgc gcggagcaac tagaggagaa cgtgcaggcg
    37921 ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca tatcgccgat
    37981 ggcgagctga atctgtggca ggcgtcgtcc gataaatgac ctgttaataa cgggcgcgcg
    38041 aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccatcac
    38101 aaatgcagaa agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatccgg
    38161 gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga
    38221 cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccact tcagcttctc
    38281 tttgttctcg gagatatcca gctgtgagac gaaattctca taaccggaaa acatcaccat
    38341 caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc
    38401 tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag
    38461 cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga
    38521 agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg
    38581 caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata tacgttaaac
    38641 ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa cgcatcgaga
    38701 gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga
    38761 cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat
    38821 ccaggcgaag atgacaccga taccggattg cggcgagaaa acctatgttg gtagcggtcg
    38881 cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc
    38941 catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga
    39001 agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc
    39061 aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc acaaggcgtt
    39121 aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc cggatattgc
    39181 agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg
    39241 gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtgtcatca ccacttcgtc
    39301 aatccaggca taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat
    39361 tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat
    39421 tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa cgcaggataa
    39481 gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg cggaactggc
    39541 ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg
    39601 cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca
    39661 tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat
    39721 gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt
    39781 gagcacctaa tgcctgatgc gtcgtgaacg ccttatccgg cctacaaacg cacgaagatt
    39841 caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc aattctactt
    39901 tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc
    39961 agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag
    40021 atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg
    40081 gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt
    40141 ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc
    40201 gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac
    40261 ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt
    40321 gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg
    40381 ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc
    40441 agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga
    40501 atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg
    40561 actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag
    40621 accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta
    40681 ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt
    40741 tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca
    40801 ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg
    40861 tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg
    40921 aagaactcta cgctcttact gaagaagatt gcccaggtga ctacagaggc caaaataagc
    40981 ccaatcatca cgcacttaac gacaatatcg gcgtactgat acatacccca gacggaaagg
    41041 tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc
    41101 gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg
    41161 ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt
    41221 tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag
    41281 tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac
    41341 tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa atagcgtgat
    41401 tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa
    41461 tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca
    41521 acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc
    41581 agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa
    41641 caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac
    41701 gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac
    41761 taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc
    41821 gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg
    41881 ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaggccgc
    41941 caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg
    42001 ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac
    42061 cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga
    42121 aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc gagttaacca
    42181 ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag
    42241 cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct
    42301 ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct
    42361 ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg
    42421 gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga
    42481 cgccggtttt gcacggattg tataacattg ccacttttgg acaattttgc agacatttta
    42541 ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta
    42601 caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag
    42661 atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta
    42721 gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct
    42781 catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc caaggggtta
    42841 tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa
    42901 gttatattca ggggagctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc
    42961 ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc
    43021 tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt
    43081 ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca
    43141 gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag
    43201 atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag
    43261 gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc
    43321 cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct
    43381 tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca
    43441 tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc
    43501 gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta
    43561 aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc
    43621 aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag actgaagtga
    43681 gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac
    43741 agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg
    43801 tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg
    43861 ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt
    43921 aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag
    43981 gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga
    44041 aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat gatgccaggc
    44101 tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc
    44161 aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag
    44221 cgacaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat
    44281 tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga
    44341 aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc
    44401 tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc
    44461 ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac
    44521 gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg cgttctcgat
    44581 caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac
    44641 ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa agtgactttc
    44701 ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct
    44761 aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa
    44821 agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgca
    44881 ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc tgcacatgtt
    44941 cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg
    45001 gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt
    45061 gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat cgaagatggt
    45121 ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg
    45181 actcaggcgc tgaacggttt gattggcact ggcgtaccgc aggactgggc aacgcatatg
    45241 ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc
    45301 ctgcctgcac tgtggaatga aaaacgcgag accaagcgcg ctaagctgct gcaatatgct
    45361 gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc
    45421 gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga ctacggtctg
    45481 gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg
    45541 ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc cgcccgctaa
    45601 gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac
    45661 ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg
    45721 ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc tggcaagcaa
    45781 gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgattg
    45841 ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag
    45901 tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac aagcgccccc
    45961 gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa
    46021 tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat
    46081 tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc
    46141 aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc
    46201 tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag accgaatcct
    46261 ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg
    46321 cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc
    46381 tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg
    46441 atttccgtct cgacaaagac gaactcggca aaattgcaaa actcgatcag ggcaagcgtc
    46501 tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt ggcggtaatg
    46561 ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc
    46621 ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa
    46681 agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata
    46741 atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc tccttcacag
    46801 accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc cagtacgcct
    46861 tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca ggcgcttacc
    46921 ctgaacgaca atcttacact accgggcaat attggctggc tggattatga tccggtttgt
    46981 gattgtcagg attttggcga tctggtgtta gaaagcgtag cgataaccca aactgacgtc
    47041 gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga aaagaccaca
    47101 cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgatgatat tgtcagcaat
    47161 catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc cgctttagct
    47221 tcattgcaga aagaacagcc agaatccttt gttgctgaac tctttgaaca tattgctgat
    47281 tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt taatgccttc
    47341 tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat agaacggcaa
    47401 tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg cgttgatgaa
    47461 attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt tacgctgacc
    47521 aatggcaaca atgaagagca agagctggtt ttacagcggc gcgaaggtaa gtgggaaatc
    47581 gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc aaaaaccgcc
    47641 gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata aataaaaggg
    47701 aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc tgcttgctca
    47761 gtggctgcgt caatgagcaa aaggtcaatc aactggcgag caatgtacaa acattaaatg
    47821 ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc tatgctgcca
    47881 aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt gattgcctgc
    47941 gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc agttgccggg
    48001 gatttttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc attcaccgga
    48061 cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg agtcgccatc
    48121 ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct ggcgacgagc ttcacgcatc
    48181 tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg
    48241 cccatcgctt ccagcgcctg acggattaac ggccagtttg ccggatcgtg gtaacgcaac
    48301 aacgctttat gcaaacgacg ctgtttgtcg cccttcggta cgaagacgcc ttcgctctta
    48361 taaccaatct tcgccagcgg ttttttcccg gtgtaataca tggtggttga gttcgccagc
    48421 ggcgatgggt agaagttctg tacctggtcg agacggaaac gatgcttttt cagccacagc
    48481 gccaaattca ccatatcttc atcacgcgta ccggggtgcg cagagatgaa atacgggatc
    48541 agatactgct ctttacctgc ctgtttcgag taggtatcga acagctcttt aaagcggtca
    48601 tagctgccca tgcccggctt catcatcttc gacaacggcc cttcttcggt atgttccgga
    48661 gcaatcttca gataaccgcc gacgtgatgg gtcgccaact ctttgatata gcgcggatct
    48721 tccacggcga tgtcataacg tacgcctgag gcgataagga tttttttaat gcctttcaga
    48781 tcacgcgcac ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat gtgcggacaa
    48841 atatccggat aaacgcacga caaacggcga caagtttgtt cagcgcgtgg cgatttgcag
    48901 cgcaacatat acatgttggc agttggccca ccgagatcgg aaatcacgcc cgtaaaacct
    48961 ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg aatcttccga acggctctga
    49021 ataatgcgcc cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa gcagccgcgc
    49081 ataatgttga ccgaaaaacg gatcatttcg taagccggaa tacgggcatt gccataggcc
    49141 ggatgtggca cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc ggtagaaagc
    49201 gggatagcag gcgggttgat ccacacatag cggtcaccgt gtttttgcat caatgcgcgg
    49261 gcgcagcctg ggttggtttc gtggtgcaga atgcgcgaag catgggcgta cagcacttta
    49321 tcgcccttca ctttctcgaa agaaggcagc aatacgtagg ttttttccca cggtttctgg
    49381 cgcggtggct gcacgattac agctttcgct tcctgctttt tcggcgcaac aggtttgtta
    49441 tccgcacacg gcaaatcttc gccatacgga tgcgggattg gggcgatttt ccctggggta
    49501 tcaagacggg tggaatccac accgctccag ccaggcagcg cctcttttac gataattgcg
    49561 gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg atgcactact
    49621 tccaccagcg gacgttcacc gttaccaaac atcagcatgt cggctttcga atctaccaga
    49681 acggaacggc gcacggtatc ggaccagtaa tcataatgcg cggtacggcg caggctagcc
    49741 tcaataccgc cgaggatcac cggcacatct ttccacgcct ctttacaacg ctgggtataa
    49801 accagcgtgg cgcgatccgg tcgcttaccc gcgacattat ctggcgtgta ggcatcatca
    49861 tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg aatccatgtt gccagcagta
    49921 acaccaaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct gctccagtcc
    49981 ggctgggcga tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc gcaaatcgcc
    50041 atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta
    50101 tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc cggtccaaaa
    50161 caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata
    50221 gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga ttataagccg
    50281 gaacgaaaga gaaatcgaaa ggtattccat actcgccctc ctcgggcgag tatgaagatt
    50341 acggtaccgg attgaccaac agttgcccaa tcgagccacg gtccgccatt tccagcgtct
    50401 gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa acaagcagct
    50461 ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg aacggcatcg
    50521 cgccattcac gttacggatc tggaacatta cgccttcaat atggaacgct tgcggctcgt
    50581 ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca atacggttga
    50641 cgtcccacag ctgtccatta atacccgggt catcacccag actgatatcg cgactgcgaa
    50701 ttggcgaacc agccatgatt tcagttggca gcaagcgcat cggaagactg tctgtgacca
    50761 gcggcagaag gccggttggg cgcagcgtta gcaccaaggt agaaaccaga atactggatg
    50821 gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc ttcaccacag gtgatcgaca
    50881 cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt gccagcgaaa
    50941 gttgcttcac cgatacagga gcaggcagga atccctgatc gccagaaatc acatgtaacg
    51001 ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc agcagtcgca
    51061 agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta accagcagcg
    51121 tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca aagttatcca
    51181 gccgtttatc ctggataatg accggaaaat catccacacc ataatggttg gggataggca
    51241 gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta tagacctgct
    51301 gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatag
    51361 gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg cccatcagcg
    51421 ggcctggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cggttgctgt
    51481 aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat
    51541 tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag
    51601 tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt agcggttgct
    51661 gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg
    51721 cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta
    51781 ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc
    51841 ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg
    51901 acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt caattggcgg
    51961 cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct tattcgaagt
    52021 tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt gaaatgctcc
    52081 agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat
    52141 ggaaatacgg cgttttttga aatgattcac gacttccgca atggtgccgt gagctttagt
    52201 gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg
    52261 gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacaa tgttcgatgc
    52321 tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc cgtagttttc
    52381 agcatctgca ggtttgcggc actcaacttt caggccgaac agtggcgcaa gacggccaaa
    52441 catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga
    52501 gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa agatatatag
    52561 cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa tcaggttagg
    52621 gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca
    52681 acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg atgacgactt
    52741 aacgtttcat ccggcgttcc ttgctagcgg gaggaaacag cgccctcccc ggcatattac
    52801 tcttcgctat caccgctgct ggcacggcga ggagagtcga tctcaacacg atcgatacgc
    52861 tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt ctgtagctct
    52921 tccggacgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc
    52981 agaacgtaca gttgcgccag accatcctcg ccacgcgcgg cttctgccga tggaatgttg
    53041 ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cacttaccgg gaacatcaac
    53101 atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat caccaccggc
    53161 ggcataacat gggcattttc cggtaaggtg atcaaagcct tacccgcacg gttacgcgct
    53221 accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat cagcagtttc
    53281 tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa cgttaatttg
    53341 ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc gatggcatag
    53401 ctacgaccgg tggaatcaac aaacactacc ggttggttac tcttaccttt caccgccgct
    53461 ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg gcctttagcg
    53521 ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg cagcatgtcg
    53581 tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg atcgtcaccg
    53641 taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt acgctcggaa
    53701 gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc accgcgaatc
    53761 ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg
    53821 gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc gttacgaatg
    53881 atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag gatatgcagg
    53941 cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc gcgacggaac
    54001 accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc atccagaccg
    54061 atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa gaggtggttc
    54121 atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg ggtcgggttc
    54181 tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt gcgcatttgc
    54241 gcagcaattt gctccagtac gcgcgcacct gaaacctgat gcggtaatgc gctgataacc
    54301 accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg tccgttctcg
    54361 tagattttac gaatctcggc gcgcgaagtg ataatttccg cttcagtcgg ataatccggc
    54421 ccctgcacga tatccagcag ctgatcgagc gtagttttcg gctggtcgat taatgcgatt
    54481 gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat gccgacggca
    54541 ataccggtgg tgccgttaag caaaatgttt ggcagacggg caggtagcat tttcggctcc
    54601 tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag ctcgctcaat
    54661 agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc gaacgatttc
    54721 ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg gtaagagaac
    54781 ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg atggtattta
    54841 cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc attcaggccc
    54901 agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc accaataaac
    54961 ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt ttccgtaaat
    55021 tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc aacttattcg
    55081 ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg tcgagtcaca
    55141 ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg catcaatgtg
    55201 ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca caaggttgcg
    55261 cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag ttgaggttat
    55321 gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatacg gttcagccca
    55381 atttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc ttgctcatgg
    55441 atatccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac atggtattcc
    55501 ttaccggaga aattataagc ccaacaacaa tacgcctgtg acattgttcg ctcaaactta
    55561 tcgcgagtga tttggctcac actccggtaa agattcccca tcacgccaac ggcacataaa
    55621 catccgtttc cagtcgctgc tctgtcaccc cttcggcaag attggtgtag tgaaacagaa
    55681 tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata atgccccata
    55741 tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa cgtccaccgg
    55801 taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta acgctgccgc
    55861 agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg ggcgtcccag gcgacgccaa
    55921 aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg ataaatttcg
    55981 ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc acagggattg
    56041 atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct ccctgcgaaa
    56101 ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt gcggatctgt
    56161 ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat ctcaaccgta
    56221 tctttcggcg tcaccgtcac accgttccag cgtttgtgat cgatatcaac attgatagta
    56281 ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat attgccgcgc
    56341 agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt tacgctgccg
    56401 ttcggcccct gaaatcctcc ggcctgactt tgcgttaccg atgggccaga aaaaccgccc
    56461 tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac tgctgcgaat
    56521 tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa gatagtcctt
    56581 aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt tacaacacgg
    56641 tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt actgatagaa
    56701 gatgacatgc tgattggtga cggcatcaaa actggcctta gtaaaatggg ttttagtgtc
    56761 gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc ttatgatgcg
    56821 gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg cgaatggcga
    56881 gagaaaggtc agcgtgagcc ggtactgaac ctgaccgcgc gtgatgcgtt agcggaacgt
    56941 gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc gttgatagaa
    57001 gtcgccgcca agctggaggc tctgatgcgc cgaaccaacg gccaggccag caacgagctg
    57061 cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc tggcgaaccc
    57121 ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa cgctggtcgg
    57181 gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga ggtcaccagt
    57241 aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga ttttattcgt
    57301 accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa cgtcttagtc
    57361 tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg ctgctttcca
    57421 gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac acccaactga
    57481 tgctgtttgc caaacggtta agtacgctcg atctcaacga aatcaacgcg gcggatcgca
    57541 tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg ctgacctttg
    57601 ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga gaagatattc
    57661 cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa gacgatcctt
    57721 ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt ggccaggaat
    57781 gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc ccgtggctgg
    57841 tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa ctcgcgccgc
    57901 tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa ccactaaacg
    57961 cgactggcgt acccagcgaa gtgcggccac tggttgagtc gctaaatcaa ctgttcgccc
    58021 gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct cacgaactac
    58081 gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct gacgatgatc
    58141 cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc gctactcgtc
    58201 tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt caggacgtcg
    58261 cggagatccc gcttgaagat ctcctacaat cgtcggtgat ggatatttac cacacggcgc
    58321 agcaggcgaa aattgacgtg cgactgacac tcaatgccca cggcatcaaa cgcaccggac
    58381 aaccgctatt gctaagtttg ttggtgcgaa atttgctgga taacggcccc ggtgtgacac
    58441 cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa accgctaccg
    58501 gcagtggact tgggctatcg attgtccagc gaatcgccaa gctgcatgac atgaatgttg
    58561 agtttgggaa tgcggaacaa ggtggatttg aggcgaaggt aagctggtaa cttctctgtc
    58621 tatgagagcc gttaaaacga ctctcataga ttttactaat agcaaaatat aaaccgtccc
    58681 caaaaaagcc accaaccaca aaccagacaa ataacgagcg agaatacata tagccaaaac
    58741 tcgaacattt cccgcataaa gagttttctt aagataagaa taataagtgg cgtaagaaga
    58801 aaaaatgctg caatcattgc ttgcaatata cgatcttcgc ctgaaacgga aggtatactg
    58861 caaatattgc ctcccgactc aagcatccag tcatacttat tgaaagcaaa aaaagaatct
    58921 attaaccaat aagtaagcat tatatatagc aatgcttcaa atattgtttt aattttctgc
    58981 tttaacatat atcagtacgc tcataaaaat ccatttataa ttatcaacat gacacgttga
    59041 tattttcatc ttttacttat tcaaagccat attttcaatc cccaaaaaaa ccgaaccaaa
    59101 cgtgtaaatt attccggtgc gagtaagaat tatgaggaat ggctatcagt attgtcattt
    59161 tcagaaaata tttatcctgc atcggtgagt cagagctaga tcagactttt gctaaattcg
    59221 caaaagactt tgcacatttt gctaatttca cagtaccgct ctgtgacgta ctatagtcga
    59281 caaacgtctc accttgagat taaaaaatga gcaacatcct gattatcaac ggcgcgaaaa
    59341 aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg gatggcacac
    59401 tgcgcgacct cgggcatgat gtccgcatcg ttcgcgccga cagcgactac aatgtcaaag
    59461 cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca ggctggtgga
    59521 tgggcgcgcc gtggacagtg aaaaaatata ttgatgatgt attcaccgaa ggtcacggga
    59581 ctctgtatgc cagcgatggt cgtacccgca aagatccgtc gaaaaaatac ggttccggca
    59641 gcctggtaca gggcaaaaaa tatatgcttt ctctaacctg gaacgcacca atggaagcct
    59701 tcaccgaaaa agatcagttc ttccacggcg ttggcgttga cggtgtgtat ctgccgttcc
    59761 ataaagcaaa ccaattcctc ggtatggaac cgctgccgac atttatcgct aatgacgtga
    59821 taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt gtggaaattt
    59881 ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta atcgcagaaa
    59941 tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt gctaaaatcg
    60001 ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg gattgcgctg
    60061 ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag cagtgggaaa
    60121 gcatcgcgca ccttgaagcg catctgcaaa ccccgcacat gaaggcgtac agcgaagccg
    60181 taaaaggcga cgtgctggag atgaatatcc gtattctgca gccagggatt taatcctgcc
    60241 ttgtttgccc ggccatcctg accgggcaat gttctttcct ttaaacctca atctccgcca
    60301 tgtcgccttt ctcttgcaac cagttgcggc gatcttccga gcgtttcttc gccagcagca
    60361 tatccatcat cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac tgcaccagac
    60421 ggcgagtgtt cggatcaagc gtggtttcac gcaattgcat cgggttcatt tcccccagac
    60481 ctttaaaacg ctggacgttc ggcttgcctt tcttgcgttt taattgctca agtacgccct
    60541 ctttctcttc ttccgtcagc gcgtaataaa cctctttccc gagatcaata cggtagagcg
    60601 gtggcagtgc gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt tttacgaaca
    60661 aagcgcagag cagcgtggca atgtgcagac catcagagtc cgcatccgcg aggatacaga
    60721 ttttgccata acgaagctgg ctcagatcgt cgctgtcagg atcgataccg atcgctaccg
    60781 aaatatcatg cacttcctgc gaggccagca cttcgtcgga agaaacttcc caggtgttaa
    60841 ggatcttacc tttcagcggc atgatcgcct gatattcacg atcgcgcgcc tgcttggcag
    60901 atccgcctgc ggagtcacct tccactagga acagttcggt acggttaagg ccctgcgcgg
    60961 tacaatcagc cagtttgcca ggcagcgccg ggccgctggt cagcttttta cgcaccactt
    61021 ttttagccgc acgcatacga cgctgggcgc tggaaatcgc catctctgcc agcaactccg
    61081 ccgcctgaac gttctggttc agccacagga tgaaggcatc tttcaccacg cccgaaacga
    61141 atgccgcaca ctgacgcgaa gagagacgct ctttggtctg cccggcaaac tgcggatcct
    61201 gcattttaac cgacagaaca taggcgcagc gatcccagat atcttccgcc gacagcttca
    61261 cgccgcgcgg cagaatgttg cggtattcac agaactcacg catcgcgtcc aacaggccct
    61321 gacgcagacc attaacatgg gtaccgccct gcatcgttgg gataaggttg acgtagcttt
    61381 cggtcagcag ttcaccgcct tccggcagcc acagtagcgc ccagtccaca gcttcagtat
    61441 cgccagcgaa attaccgata aacggttttt ctggcagcgt cggtaaaccg tttaccgctt
    61501 ccgccaggta atcattcaga ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct
    61561 catctttaaa ggtaatctca acgccggggc acagtactgc tttggccttc agcacatgcg
    61621 tcaggcgtga aacagaaaaa cgcgggctgt caaagaaggt ttcatccggc cagaagtgca
    61681 cgctggtacc ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt
    61741 cgccattttc aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta acttctacgc
    61801 gcttcgacaa ggcgttaact accgaaatcc ccacgccatg caggccgcca gagaactggt
    61861 aatttttgtt agagaattta ccgcctgcat gcagacggca aagaatcagt tcaaccgccg
    61921 gtactccttc ttccgggtga atatccaccg gcatcccgcg cccatcgtca atcacttcta
    61981 acgactggtc agcatgtaag attacatcca cgcgttttgc gtgacccgcc agtgcttcat
    62041 cgacactgtt atcaataact tcttgcccca aatggttagg gcgagtggta tcggtataca
    62101 tccccggacg gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca tcagcgttat
    62161 aagtttgcgt catggtttaa gttagcaatt cgagttgatc gtcagagatg gtgcagacca
    62221 agaaaatcga cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct
    62281 tctatgacag tctggcggca ggaagcgtag tacgccaccg cctggcggta atccagcact
    62341 tcatctcccg tctgttgcag cagccagatc aaatccggcg cttccagcgg gtcaatctgc
    62401 atgactttga gatcgtaaat atggcgtgac tctagcacat attgctgccc ggtgtagggg
    62461 ttctcgttct gaccgagata gtccgtcagc aattcaaacg ggcgtaccgc cgggtttacc
    62521 accactgcgg gcagcataaa acattgcgac aaccaggtgg cgtaatatcc ccccagtgac
    62581 gaaccgacaa tacccagcga atcgccgcca tgttccagga caatggattc cagcagttct
    62641 gccgcgtcgg aaggatacgg cggcaactgc ggaatgatca tctcaacgtc agggtgatgt
    62701 tccgccagcc agttttttaa caagctcgct tttgcagagc gcggcgagct gttgaaaccg
    62761 tgtaaataaa gaagcgtaga catcagtagc cttctgaagc ggtatcaggt tggaaacgtg
    62821 tgtccgccag gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac tcgagagtac
    62881 gccagccggg cgcgatagta tccagcgtaa agttggaaca gtgcggctta aactgcacac
    62941 aggtcgacgg cgttgccagc aggcggcgac cgttccagtc gagatccagc tcctgatgaa
    63001 tatgaccgca cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc
    63061 ccgcgttacg cagactgtgt tgatcgagcc aactacaacc cgcaggtagc ggatgatgat
    63121 gcagcagcag caacgtatgg cgttctggcg catcggccag tttacgttcc agccactcaa
    63181 gctgaaactc gctcagctca ccgtgcggca cgccaaacac ctggctatcc agcaacagga
    63241 tttgccattg ctcaccaata aacacccgct tcgccgggga gatacccgca tcctgtaacg
    63301 cgctgtacat cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa
    63361 aacttgcgat gccttcagcg aaatgctgat aggccgcaga ggattgatcc tgcgctaaat
    63421 cacctgtcgc gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc tccagcaccg
    63481 cctggtagct ttcccaggtg tttaccccta acagggcttc gtgcttttgt gcaaacaggt
    63541 gagtatcggt aatttgtaaa atccttcctc tggcctcacc agccagagga agggttaaca
    63601 ggctttccaa atggtgtcct taggtttcac gacgctagta aaccggaatc gccatcgctc
    63661 catgtgctaa acagtatcgc aaccagtccg ctaaaaactg gttaatttga tgcttttcgt
    63721 cgcgttgatg caacttttta ttaggataat cataccgcgc tttgaagcga aaaatctgct
    63781 ggcttgaaca cacttcagcc accatcgcgt catgatacag acgcaccgtc attgacggaa
    63841 ggctccagta actgatcgcg ggcgcagtct gttctattgt cactagggta gtgtatcggg
    63901 tcgattccac aatcgtcagc cgatattgtg cgtttgccac ctgatagctt acagtttcgc
    63961 cgggtgcgtc attgcgcggt aacaaacggc gcaattgtga aaagttcatc tcgcacaggc
    64021 gcatcatttc aggaaagtca ggtgtgtaac gcttcattta tgcccactca ttttttaacg
    64081 cttgatgatg cagctgcagc cattgcaaag cgatgaccga cgctgcgttg tcgattttcc
    64141 cctcttctac ccactggtat gcctgttccc ggcttaccac atgaacgcga atatcttcgt
    64201 tttcatcagc cagaccatga ataccgcttg cggtcgtggc gtccacttcg cccaccataa
    64261 ttgacgaacg ctcactggtg ccccccgggc ttgccaggaa acttaacacc ggtttggtcc
    64321 gtttgactat cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct tccacacttt
    64381 caccctcttc aatcatcccg gcaaccatct ccagtagcca gggggtttcg ctggtgtcgt
    64441 acgcggcaat ccgaatctgc tcaatcagca caacttcatc acgcactggg tcaaagggta
    64501 gcaagactgc ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga ctcatttgcc
    64561 cgttgaatag acgatgacga aatctataaa gatctaatga aaaaaagccg cgataaagtg
    64621 tttctcgtgc aataatttct acatcgtttt tgccaaatgt aacgggcagg ttgtctggct
    64681 taagcattgt taatgtccta gcactaatag tgaattaaat gtgaatttca gcgacgtttg
    64741 actgccgttt gagcagtcat atgttaaatt gaggcacatt aacgccctat ggcacgtaac
    64801 gccaaccttt tgcggtagcg gcttctgcta gaatccgcaa taattttaca gtttgatcgc
    64861 gctaaatact gcttcaccac aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg
    64921 cctgagcctt tctgggttca gttcgttgag ccaggccgag aacctgatgc aagtttatca
    64981 gcaagcacgc cttagtaacc cggaattgcg taagtctgcc gccgatcgtg atgctgcctt
    65041 tgaaaaaatt aatgaagcgc gcagtccatt actgccacag ctaggtttag gtgcagatta
    65101 cacctatagc aacggctacc gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc
    65161 cctgcagtta actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga
    65221 aaaagcagca gggattcagg acgtcacata tcagaccgat cagcaaacct tgatcctcaa
    65281 caccgcgacc gcttatttca acgtgttgaa tgctattgac gttctttcct atacacaggc
    65341 acaaaaagaa gcgatctacc gtcaattaga tcaaaccacc caacgtttta acgtgggcct
    65401 ggtagcgatc accgacgtgc agaacgcccg cgcgcagtac gataccgtgc tggcgaacga
    65461 agtgaccgca cgtaataacc ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa
    65521 ctactatccg gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc
    65581 ggttaacgcg ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac aggcacgctt
    65641 gagccaggac ctggagcgcg agcaaattcg ccaggcgcag gatggtcact taccgactct
    65701 ggatttaacg gcttctaccg ggatttctga cacctcttat agcggttcga aaacccgtgg
    65761 tgccgctggt acccagtatg acgatagcaa tatgggccag aacaaagttg gcctgagctt
    65821 ctcgctgccg atttatcagg gcggaatggt taactcgcag gtgaaacagg cacagtacaa
    65881 ctttgttggt gccagtgagc aactggaaag cgcacatcgt agcgtcgtgc aaaccgtacg
    65941 ttcctccttc aacaacatta atgcttctat cagtagtatt aacgcctaca aacaagccgt
    66001 agtttccgcg caaagctcat tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac
    66061 cattgttgat gtgttggatg cgaccaccac gctgtacaac gccaagcaag agctggcgaa
    66121 tgcgcgttat aactacctga ttaatcagct gaatattaag tcagccctgg gtacgttgaa
    66181 cgagcaggat ttgctggcac tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga
    66241 aaacgttgcc ccgcaaacgc cggaacagaa tgctattgct gatggttatg cgcctgatag
    66301 cccggcaccc gtcgttcagc aaacatccgc acgcactacc accagtaacg gtcataaccc
    66361 tttccgtaac tgatgacgac gacggggctt cggccccgtc tgaacgtaag gcaacgtaaa
    66421 gatacgggtt atctgccgca ttcttccccc ttctcgcttc aatttcgacc agccatcctc
    66481 tattctgatg ggtatttacc actggtcccg gaagacaaaa atgaaacgga caaaatccat
    66541 acgccacgca tcgttccgca aaaactggag cgcacgccat ctgacaccag tcgctctcgc
    66601 ggttgccact gtttttatgc tggctggctg tgaaaagagt gatgaaacag tgtctctcta
    66661 tcaaaatgct gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc
    66721 gtacaacaat gcgctgaaag aagccgaacg tactgcgccg aaatacgcca cccgtgaaga
    66781 ctgtgttgct gaatttggtg aaggtcagtg tcagcaagca ccagcccagg ctggcatggc
    66841 accagaaaac caggcgcagg ctcagcaatc cagcgggagt ttctggatgc cgctgatggc
    66901 cggttacatg atggggcgtc tgatgggcgg cggcgcggga tttgcacagc agccgctgtt
    66961 ctcctcgaaa aacccggcca gtccggctta cggtaaatat accgacgcga cgggtaaaaa
    67021 ctatggcgca gcccagccag gccgcaccat gaccgtaccg aagacggcaa tggcaccaaa
    67081 accggcgacc accactaccg ttacccgtgg cggttttggt gaatctgttg ccaaacaaag
    67141 cactatgcag cgtagcgcaa ccggcacctc ttctcgttca atgggtggct gataccgatg
    67201 gaaagagtca gtattaccga gcgcccggac tggcgtgaga aagcccacga atacggtttc
    67261 aattttcaca ccatgtacgg cgagccgtac tggtgtgaag atgcttacta caagttgacc
    67321 ctcgcccagg ttgaaaagct ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa
    67381 gtggtggaaa aagtgatcgc cagcgatgag ctgatgacca aattccgcat accaaaacac
    67441 acctggagtt ttgtgcgtca atcatggctg actcaccagc catcgcttta ttcgcgtctt
    67501 gatctggcgt gggatggcac tggcgaacct aaacttctgg aaaataacgc cgatacgcca
    67561 acgtcactat acgaggcggc gttctttcag tggatctggc tggaagatca gcttaacgcc
    67621 ggtaacttgt cggagggcag cgaccagttt aacagtctgc aagaaaaact gatcgatcgc
    67681 ttcgttgagc tgcgtgaaca gtatggcttc cagttgctgc atctcacctg ctgtcgcgac
    67741 acggtggaag atcgcggaac cattcagtat ttgcaggact gcgcaaccga agctgaaatc
    67801 gccaccgagt tcctctacat cgacgagata ggtttaggcg aaaaaggcca gttcacggat
    67861 ttacaggatc aggtgatttc taacctgttc aagctttatc cgtgggaatt tatgctgcgc
    67921 gaaatgttct ccaccaagct ggaagatgct ggcgtacgct ggctggaacc ggcgtggaag
    67981 agcattatct ccaacaaggc acttctaccg ctactgtggg agatgttccc gaatcacccg
    68041 aacctgctgc ccgcttattt tgcggaagat gatcatccgc aaatggaaaa atatgtggtt
    68101 aaaccgatct tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt
    68161 gaagcagcgg aaggtccgta tggcgaagaa gggatgattg ttcagcaatt ccacccgtta
    68221 ccgaaattcg gcgacagcta tatgctgatt ggtagctggc tggtgaacga tcaacctgcc
    68281 ggaattggca ttcgtgaaga ccgtgcgttg atcacccagg atatgtctcg gttttatcca
    68341 catatttttg ttgaataagc cacgataccg gatggcactc gccatccggt aattgttagc
    68401 ctatctgcac cgacagcata cttaaactgc ccatttctat accatcgacc ggaatggtaa
    68461 ttggctcctg cccatcccac acacctaaca catacaacaa cggcagatag tgctctggcg
    68521 ttgggttcga taacgcgcca ccttcatggt cgagataatt caccagagga tgctgctcta
    68581 ccggcccttg ccacgtcaga ttcgctttca catactcatt aaacgacgtc gcccacggat
    68641 acggtgaact atcaccgtgc cacttcactg tgcgcaggtt atgcaccacg ttaccgctgg
    68701 cgaccaacat tattccttca tcacgcagcg ccgcgagttt gcgccccatt tcgaaatgcc
    68761 aggcgacagg tttgctactg tcgatactca actgcaccat agggatatcg gcgtcaggat
    68821 acatcttaat cagcacgccc cacgagccgt ggtcaaagcc ccaggcttct ttatccagcg
    68881 tcaccgggat cggcgctaac agctcaacca gacgctgtgc cagcgcaggc gaacccggag
    68941 caggataatg cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg
    69001 gcgtctccat cgcggtcact cctgttccac gggtaaacca gtgagccgaa accaccacaa
    69061 tcgcttgcgg gcgtggcaat gtcatcccca acttctgcca gctgcgggta tacaaattat
    69121 cttccagcac gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag
    69181 acatgatgat atccttaact aaaggtgtca ttttgatatc ctcacaatac gcttgttcgg
    69241 cggagtaaga acccggataa caatgatgat gatcatcagt tattttgacg atctgcctga
    69301 aggtgaagat ttataaggag ttgtcgatgt cagtacctct cattctgacc atactggcgg
    69361 gggcagccac gtttattggc gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct
    69421 tactggcgtt ttcgctaggt tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa
    69481 tgcttcctgc cgcactagca gctgaaggaa tgtcgccggt gttgggttat ggaatgttta
    69541 tcttcggtct gcttggctac tttggcctgg accgcatgtt gccgcatgcg catccgcagg
    69601 atttaatgca aaaatcggtg cagctgttgc caaaatcgat caagcgcaca gccattctgc
    69661 tcactctcgg catcagtctg cataacttcc cggaagggat tgccaccttt gtcacggcga
    69721 gcagcaacct ggagctggga tttggcatcg cactggccgt cgcgttgcac aatatccctg
    69781 aaggtctggc agtggcaggc ccggtttatg cggcaacggg gtctaaacgc accgcgattc
    69841 tgtgggcggg gatttctgga ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc
    69901 tcggtagcat gatttccccg gtggtcatgg cggcaatcat ggcggcggtt gcaggaatta
    69961 tggtggcgct ctcggttgat gaattaatgc cgctcgccaa agagattgac cctaataata
    70021 accccagcta tggcgtacta tgtggaatgt cagtgatggg attcagttta gtgctgctac
    70081 aaacggcggg aattggttaa aaacaacatc agaagttgtc cagcacatgg acaacttctg
    70141 ccagcttcac gaaagcctta ttctaccttt tcggctgtct cttctctcgt actgtttaag
    70201 tatttgttcc gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg acaataacgt
    70261 ttcaaatgca ggaaataacc caccacaacc agagcaatca atacaatcca gtaccaggcg
    70321 ataaacatcc ttttaccctt aatatttaac aatactaaac atttagcgca taaatttcac
    70381 atatcctttt tcggatatat tcatatggtc gcataataac ggaacaatta tatgattttg
    70441 atcataatga aaaagaatgc ctgatattaa tgaaattttc tcatagaaag agaagcggaa
    70501 ttaacatctt gcatagcagg gcaaaataaa aaccgaagcc agctacaact tcggtttctt
    70561 gattaaggca gtaattaagc agcggttttc agctggcttt acgctcatgt gcctgacggt
    70621 atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca aactcaatgc
    70681 actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagc acaccagccg
    70741 gtttaaagcc tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg ccacgcgtca
    70801 gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc agatcagaag
    70861 gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg gcagaaacac
    70921 cggtagtcac accttcagcc gcttcaatgg tcacggtaaa accggtgcca taggcgctgg
    70981 tgttattttc taccatcatt ggcagatcaa gttgtttacg gcgatcttca gtgatgcaca
    71041 ggcaaacaat accgctaccg tggcgaatgg tcagcgccat ctgctcaacg gtcatggttt
    71101 ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc atcatcaagc accattacac
    71161 cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc gtaccaaaag
    71221 aggaaagtag cgtctgattc atggtaaaaa aacctcacta aaattatggt taccagaatc
    71281 agggcagtct taggagtggc ggcatatagc caaaataacg tgagcgggtc catgcccgac
    71341 agaatcgtta ctctctccca tccggactct aaccgtcggc cccggaatta caccggatct
    71401 gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt caactgagtt gatttaccgc
    71461 cggtggggaa tttcgccccg ccctgagaat aagcggattc actataacgc taatgattag
    71521 cggcagcaac gcatagcttc acataattct ggtttatgac ttacccttat cgcactacaa
    71581 tggtactcaa cacctatcag tacagggaaa ctacaatgat tgacccgaaa aaaattgagc
    71641 aaatcgctcg ccaggttcac gaatcaatgc ctaaaggaat cagggagttc ggggaagatg
    71701 tggagaaaaa aatccgccaa accctacaag ctcagctgac gcgcctcgac cttgtaagcc
    71761 gtgaagagtt cgacgtccaa acgcaagtcc tgttacggac tcgtgagaaa ctggcgctgc
    71821 ttgaacaaag aatcagcgaa ctggaaaatc gcagtactga gatcaaaaag cagccagatc
    71881 cagagactct ccccccaacg ctgtaaacat ttgtctttaa tgcactgtaa attatgaatt
    71941 aatcctctaa tttacagtgt tttctattac attatcgctt aatcccattc agagtatcat
    72001 cacaaaaatg aatgattgca attgaaaata taattgttat gtattcttta agttttactt
    72061 aatcgatatt tagactggcc c
//
LOCUS       DAEEDS010000007        70988 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEEDS010000007 DAEEDS010000000
VERSION     DAEEDS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70988
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.007"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..795
                     /locus_tag="KLH32_000489"
     CDS             <1..795
                     /locus_tag="KLH32_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0137458.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="KLH32_000490"
     CDS             806..1897
                     /locus_tag="KLH32_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0137459.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KLH32_000491"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KLH32_000491"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBE0137460.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="KLH32_000492"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="KLH32_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBE0137461.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="KLH32_000493"
     CDS             4859..5254
                     /locus_tag="KLH32_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBE0137462.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="KLH32_000494"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="KLH32_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBE0137463.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="KLH32_000495"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="KLH32_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBE0137464.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="KLH32_000496"
     CDS             complement(6563..7603)
                     /locus_tag="KLH32_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE0137465.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="KLH32_000497"
     CDS             complement(7676..8311)
                     /locus_tag="KLH32_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBE0137466.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="KLH32_000498"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="KLH32_000498"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBE0137467.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="KLH32_000499"
     CDS             complement(8937..9380)
                     /locus_tag="KLH32_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBE0137468.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="KLH32_000500"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="KLH32_000500"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBE0137469.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="KLH32_000501"
     CDS             complement(9720..10223)
                     /locus_tag="KLH32_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0137470.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="KLH32_000502"
     CDS             complement(10217..10741)
                     /locus_tag="KLH32_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBE0137471.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="KLH32_000503"
     CDS             10950..11945
                     /locus_tag="KLH32_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE0137472.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTSLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="KLH32_000504"
     CDS             11954..12832
                     /locus_tag="KLH32_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE0137473.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="KLH32_000505"
     CDS             12913..13920
                     /locus_tag="KLH32_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBE0137474.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KLH32_000506"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KLH32_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBE0137475.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KLH32_000507"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KLH32_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBE0137476.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KLH32_000508"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KLH32_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBE0137477.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KLH32_000509"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KLH32_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBE0137478.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KLH32_000510"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KLH32_000510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBE0137479.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KLH32_000511"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KLH32_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBE0137480.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KLH32_000512"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KLH32_000512"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBE0137481.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KLH32_000513"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KLH32_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBE0137482.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KLH32_000514"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KLH32_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBE0137483.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KLH32_000515"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KLH32_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBE0137484.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KLH32_000516"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KLH32_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBE0137485.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="KLH32_000517"
     tRNA            complement(27654..27730)
                     /locus_tag="KLH32_000517"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="KLH32_000518"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="KLH32_000518"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBE0137486.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31054)
                     /locus_tag="KLH32_000519"
                     /pseudo
     CDS             complement(29429..31054)
                     /locus_tag="KLH32_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245267.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="KLH32_000520"
     tRNA            complement(31513..31599)
                     /locus_tag="KLH32_000520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KLH32_000521"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KLH32_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBE0137487.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KLH32_000522"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KLH32_000522"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBE0137488.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KLH32_000523"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KLH32_000523"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBE0137489.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KLH32_000524"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KLH32_000524"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBE0137490.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KLH32_000525"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KLH32_000525"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBE0137491.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="KLH32_000526"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="KLH32_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBE0137492.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KLH32_000527"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KLH32_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBE0137493.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="KLH32_000528"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="KLH32_000528"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBE0137494.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KLH32_000529"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KLH32_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBE0137495.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="KLH32_000530"
     CDS             complement(41211..42176)
                     /locus_tag="KLH32_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0137496.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KLH32_000531"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KLH32_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBE0137497.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KLH32_000532"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KLH32_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBE0137498.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="KLH32_000533"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="KLH32_000533"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBE0137499.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="KLH32_000534"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="KLH32_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBE0137500.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KLH32_000535"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KLH32_000535"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBE0137501.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KLH32_000536"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KLH32_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBE0137502.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KLH32_000537"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KLH32_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBE0137503.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KLH32_000538"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KLH32_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBE0137504.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KLH32_000539"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KLH32_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBE0137505.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KLH32_000540"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KLH32_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBE0137506.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KLH32_000541"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KLH32_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBE0137507.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="KLH32_000542"
     CDS             49714..50679
                     /locus_tag="KLH32_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBE0137508.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="KLH32_000543"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="KLH32_000543"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBE0137509.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="KLH32_000544"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="KLH32_000544"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBE0137510.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="KLH32_000545"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="KLH32_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBE0137511.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="KLH32_000546"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="KLH32_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBE0137512.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="KLH32_000547"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="KLH32_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBE0137513.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="KLH32_000548"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="KLH32_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBE0137514.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="KLH32_000549"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="KLH32_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBE0137515.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="KLH32_000550"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="KLH32_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBE0137516.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="KLH32_000551"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="KLH32_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBE0137517.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="KLH32_000552"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="KLH32_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBE0137518.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="KLH32_000553"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="KLH32_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBE0137519.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KLH32_000554"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KLH32_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KLH32_000555"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KLH32_000555"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBE0137520.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KLH32_000556"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KLH32_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBE0137521.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="KLH32_000557"
     CDS             complement(62540..63469)
                     /locus_tag="KLH32_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBE0137522.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="KLH32_000558"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="KLH32_000558"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBE0137523.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="KLH32_000559"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="KLH32_000559"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBE0137524.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="KLH32_000560"
     CDS             70219..70698
                     /locus_tag="KLH32_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBE0137525.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            70773..70988
                     /locus_tag="KLH32_000561"
                     /pseudo
     CDS             70773..70988
                     /locus_tag="KLH32_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        17402 a        19237 c        17617 g        16732 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccgccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgc
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcagt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acgccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctactgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgcatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa cctcccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tcgtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaagctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagttaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaaggaaatg aggtagcctg agtttaacgg acactccttc
    70741 ctgaaataga atggcatcag aaggagctaa taatgagcag aaaaacccaa cgttactcta
    70801 aagagttcaa agccgaagct gtcagaacgg ttcttgaaaa tcaactttcg atcagtgaag
    70861 gcgcttcccg attatctctt cctgaaggca ctttaggaca atgggttacc gcacggtggc
    70921 tgaactggaa tctgaaattc tgcaactgcg taaggcgtta aatgaagctc gccttgagcg
    70981 agatatat
//
LOCUS       DAEEDS010000008        67101 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEEDS010000008 DAEEDS010000000
VERSION     DAEEDS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67101
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.008"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="KLH32_000562"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="KLH32_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBE0137526.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="KLH32_000563"
     CDS             complement(1150..1236)
                     /locus_tag="KLH32_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBE0137527.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KLH32_000564"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KLH32_000564"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE0137528.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KLH32_000565"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KLH32_000565"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBE0137529.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="KLH32_000566"
     CDS             3585..3629
                     /locus_tag="KLH32_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137530.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KLH32_000567"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KLH32_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBE0137531.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="KLH32_000568"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="KLH32_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBE0137532.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="KLH32_000569"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="KLH32_000569"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBE0137533.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="KLH32_000570"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="KLH32_000570"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBE0137534.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="KLH32_000571"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="KLH32_000571"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBE0137535.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="KLH32_000572"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="KLH32_000572"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBE0137536.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KLH32_000573"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KLH32_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBE0137537.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KLH32_000574"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KLH32_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBE0137538.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="KLH32_000575"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="KLH32_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBE0137539.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="KLH32_000576"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="KLH32_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBE0137540.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="KLH32_000577"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="KLH32_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBE0137541.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="KLH32_000578"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="KLH32_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBE0137542.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="KLH32_000579"
     CDS             complement(16981..17745)
                     /locus_tag="KLH32_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0137543.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KLH32_000580"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KLH32_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBE0137544.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="KLH32_000581"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="KLH32_000581"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBE0137545.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="KLH32_000582"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="KLH32_000582"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBE0137546.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="KLH32_000583"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="KLH32_000583"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBE0137547.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="KLH32_000584"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="KLH32_000584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBE0137548.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="KLH32_000585"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="KLH32_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBE0137549.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="KLH32_000586"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="KLH32_000586"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBE0137550.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KLH32_000587"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KLH32_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBE0137551.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="KLH32_000588"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="KLH32_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBE0137552.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="KLH32_000589"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="KLH32_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBE0137553.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="KLH32_000590"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="KLH32_000590"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBE0137554.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="KLH32_000591"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="KLH32_000591"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBE0137555.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="KLH32_000592"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="KLH32_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBE0137556.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="KLH32_000593"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="KLH32_000593"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBE0137557.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KLH32_000594"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KLH32_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBE0137558.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KLH32_000595"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KLH32_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBE0137559.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KLH32_000596"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KLH32_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBE0137560.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="KLH32_000597"
     CDS             complement(40421..41815)
                     /locus_tag="KLH32_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0137561.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KLH32_000598"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KLH32_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBE0137562.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="KLH32_000599"
     CDS             complement(42158..43444)
                     /locus_tag="KLH32_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0137563.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="KLH32_000600"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="KLH32_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="KLH32_000601"
     CDS             complement(44451..45221)
                     /locus_tag="KLH32_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBE0137564.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="KLH32_000602"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="KLH32_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBE0137565.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="KLH32_000603"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="KLH32_000603"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBE0137566.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="KLH32_000604"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="KLH32_000604"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBE0137567.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="KLH32_000605"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="KLH32_000605"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBE0137568.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="KLH32_000606"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="KLH32_000606"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBE0137569.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="KLH32_000607"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="KLH32_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBE0137570.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KLH32_000608"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KLH32_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBE0137571.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KLH32_000609"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KLH32_000609"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBE0137572.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KLH32_000610"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KLH32_000610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBE0137573.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KLH32_000611"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KLH32_000611"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBE0137574.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KLH32_000612"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KLH32_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBE0137575.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KLH32_000613"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KLH32_000613"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBE0137576.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KLH32_000614"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KLH32_000614"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0137577.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KLH32_000615"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KLH32_000615"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBE0137578.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KLH32_000616"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KLH32_000616"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBE0137579.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KLH32_000617"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KLH32_000617"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBE0137580.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="KLH32_000618"
     CDS             complement(66187..66405)
                     /locus_tag="KLH32_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBE0137581.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="KLH32_000619"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="KLH32_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBE0137582.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16290 a        18259 c        17267 g        15285 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggtcgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct t
//
LOCUS       DAEEDS010000009        66140 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEEDS010000009 DAEEDS010000000
VERSION     DAEEDS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66140
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.009"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..88
                     /locus_tag="KLH32_000620"
     CDS             <1..88
                     /locus_tag="KLH32_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0137583.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(236..715)
                     /gene="folK"
                     /locus_tag="KLH32_000621"
     CDS             complement(236..715)
                     /gene="folK"
                     /locus_tag="KLH32_000621"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBE0137584.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KLH32_000622"
     CDS             complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KLH32_000622"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBE0137585.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KLH32_000623"
     CDS             complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KLH32_000623"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBE0137586.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KLH32_000624"
     CDS             complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KLH32_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBE0137587.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KLH32_000625"
     CDS             complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KLH32_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBE0137588.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KLH32_000626"
     CDS             complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KLH32_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBE0137589.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5088..7517
                     /gene="hrpB"
                     /locus_tag="KLH32_000627"
     CDS             5088..7517
                     /gene="hrpB"
                     /locus_tag="KLH32_000627"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBE0137590.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7713..10247
                     /gene="mrcB"
                     /locus_tag="KLH32_000628"
     CDS             7713..10247
                     /gene="mrcB"
                     /locus_tag="KLH32_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBE0137591.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10467..12710
                     /gene="fhuA"
                     /locus_tag="KLH32_000629"
     CDS             10467..12710
                     /gene="fhuA"
                     /locus_tag="KLH32_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBE0137592.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12761..13558
                     /gene="fhuC"
                     /locus_tag="KLH32_000630"
     CDS             12761..13558
                     /gene="fhuC"
                     /locus_tag="KLH32_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBE0137593.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13558..14448
                     /gene="fhuD"
                     /locus_tag="KLH32_000631"
     CDS             13558..14448
                     /gene="fhuD"
                     /locus_tag="KLH32_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBE0137594.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14445..16427
                     /gene="fhuB"
                     /locus_tag="KLH32_000632"
     CDS             14445..16427
                     /gene="fhuB"
                     /locus_tag="KLH32_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBE0137595.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KLH32_000633"
     CDS             complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KLH32_000633"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBE0137596.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17967..19388
                     /gene="clcA"
                     /locus_tag="KLH32_000634"
     CDS             17967..19388
                     /gene="clcA"
                     /locus_tag="KLH32_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBE0137597.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19412..19477
                     /locus_tag="KLH32_000635"
     CDS             19412..19477
                     /locus_tag="KLH32_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137598.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19470..19814
                     /gene="erpA"
                     /locus_tag="KLH32_000636"
     CDS             19470..19814
                     /gene="erpA"
                     /locus_tag="KLH32_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBE0137599.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19861..20484)
                     /locus_tag="KLH32_000637"
     CDS             complement(19861..20484)
                     /locus_tag="KLH32_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBE0137600.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KLH32_000638"
     CDS             complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KLH32_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBE0137601.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KLH32_000639"
     CDS             complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KLH32_000639"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBE0137602.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22097..23614
                     /gene="dgt"
                     /locus_tag="KLH32_000640"
     CDS             22097..23614
                     /gene="dgt"
                     /locus_tag="KLH32_000640"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBE0137603.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23744..25168
                     /gene="degP"
                     /locus_tag="KLH32_000641"
     CDS             23744..25168
                     /gene="degP"
                     /locus_tag="KLH32_000641"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBE0137604.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25323..26479
                     /gene="cdaR"
                     /locus_tag="KLH32_000642"
                     /pseudo
     CDS             25323..26479
                     /gene="cdaR"
                     /locus_tag="KLH32_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26568..26954)
                     /locus_tag="KLH32_000643"
     CDS             complement(26568..26954)
                     /locus_tag="KLH32_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBE0137605.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KLH32_000644"
     CDS             complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KLH32_000644"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBE0137606.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KLH32_000645"
     CDS             complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KLH32_000645"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBE0137607.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30857..31651)
                     /gene="map"
                     /locus_tag="KLH32_000646"
     CDS             complement(30857..31651)
                     /gene="map"
                     /locus_tag="KLH32_000646"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBE0137608.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32019..32744
                     /gene="rpsB"
                     /locus_tag="KLH32_000647"
     CDS             32019..32744
                     /gene="rpsB"
                     /locus_tag="KLH32_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBE0137609.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33002..33853
                     /gene="tsf"
                     /locus_tag="KLH32_000648"
     CDS             33002..33853
                     /gene="tsf"
                     /locus_tag="KLH32_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBE0137610.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34000..34725
                     /gene="pyrH"
                     /locus_tag="KLH32_000649"
     CDS             34000..34725
                     /gene="pyrH"
                     /locus_tag="KLH32_000649"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBE0137611.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35017..35574
                     /gene="frr"
                     /locus_tag="KLH32_000650"
     CDS             35017..35574
                     /gene="frr"
                     /locus_tag="KLH32_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBE0137612.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35666..36862
                     /gene="ispC"
                     /locus_tag="KLH32_000651"
     CDS             35666..36862
                     /gene="ispC"
                     /locus_tag="KLH32_000651"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBE0137613.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            37051..37809
                     /gene="ispU"
                     /locus_tag="KLH32_000652"
                     /gene_synonym="uppS"
     CDS             37051..37809
                     /gene="ispU"
                     /locus_tag="KLH32_000652"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBE0137614.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37822..38679
                     /gene="cdsA"
                     /locus_tag="KLH32_000653"
     CDS             37822..38679
                     /gene="cdsA"
                     /locus_tag="KLH32_000653"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE0137615.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38691..40043
                     /gene="rseP"
                     /locus_tag="KLH32_000654"
     CDS             38691..40043
                     /gene="rseP"
                     /locus_tag="KLH32_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBE0137616.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40073..42505
                     /gene="bamA"
                     /locus_tag="KLH32_000655"
     CDS             40073..42505
                     /gene="bamA"
                     /locus_tag="KLH32_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBE0137617.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42627..43112
                     /gene="skp"
                     /locus_tag="KLH32_000656"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42627..43112
                     /gene="skp"
                     /locus_tag="KLH32_000656"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBE0137618.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43116..44141
                     /gene="lpxD"
                     /locus_tag="KLH32_000657"
     CDS             43116..44141
                     /gene="lpxD"
                     /locus_tag="KLH32_000657"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBE0137619.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44246..44701
                     /gene="fabZ"
                     /locus_tag="KLH32_000658"
     CDS             44246..44701
                     /gene="fabZ"
                     /locus_tag="KLH32_000658"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBE0137620.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44705..45493
                     /gene="lpxA"
                     /locus_tag="KLH32_000659"
     CDS             44705..45493
                     /gene="lpxA"
                     /locus_tag="KLH32_000659"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBE0137621.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45493..46641
                     /gene="lpxB"
                     /locus_tag="KLH32_000660"
     CDS             45493..46641
                     /gene="lpxB"
                     /locus_tag="KLH32_000660"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBE0137622.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46638..47234
                     /gene="rnhB"
                     /locus_tag="KLH32_000661"
     CDS             46638..47234
                     /gene="rnhB"
                     /locus_tag="KLH32_000661"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBE0137623.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47271..50753
                     /gene="dnaE"
                     /locus_tag="KLH32_000662"
     CDS             47271..50753
                     /gene="dnaE"
                     /locus_tag="KLH32_000662"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBE0137624.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50766..51725
                     /gene="accA"
                     /locus_tag="KLH32_000663"
     CDS             50766..51725
                     /gene="accA"
                     /locus_tag="KLH32_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBE0137625.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51824..53965
                     /gene="ldcC"
                     /locus_tag="KLH32_000664"
     CDS             51824..53965
                     /gene="ldcC"
                     /locus_tag="KLH32_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBE0137626.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54022..54411
                     /locus_tag="KLH32_000665"
     CDS             54022..54411
                     /locus_tag="KLH32_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0137627.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54476..55774
                     /gene="tilS"
                     /locus_tag="KLH32_000666"
     CDS             54476..55774
                     /gene="tilS"
                     /locus_tag="KLH32_000666"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBE0137628.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KLH32_000667"
     CDS             complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KLH32_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBE0137629.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56070..56270)
                     /locus_tag="KLH32_000668"
     CDS             complement(56070..56270)
                     /locus_tag="KLH32_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBE0137630.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56436..56981
                     /locus_tag="KLH32_000669"
     CDS             56436..56981
                     /locus_tag="KLH32_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBE0137631.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56978..57400
                     /gene="arfB"
                     /locus_tag="KLH32_000670"
     CDS             56978..57400
                     /gene="arfB"
                     /locus_tag="KLH32_000670"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE0137632.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57414..58124
                     /gene="nlpE"
                     /locus_tag="KLH32_000671"
                     /gene_synonym="cutF"
     CDS             57414..58124
                     /gene="nlpE"
                     /locus_tag="KLH32_000671"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBE0137633.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKTVLLSVEGHFTLEANPDTGVPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58279..59103)
                     /locus_tag="KLH32_000672"
     CDS             complement(58279..59103)
                     /locus_tag="KLH32_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBE0137634.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KLH32_000673"
     CDS             complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KLH32_000673"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBE0137635.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KLH32_000674"
     CDS             complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KLH32_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBE0137636.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KLH32_000675"
     CDS             complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KLH32_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBE0137637.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KLH32_000676"
     CDS             complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KLH32_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBE0137638.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KLH32_000677"
     CDS             complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KLH32_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBE0137639.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KLH32_000678"
     CDS             complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KLH32_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBE0137640.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64931..65506
                     /gene="gmhB"
                     /locus_tag="KLH32_000679"
     CDS             64931..65506
                     /gene="gmhB"
                     /locus_tag="KLH32_000679"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBE0137641.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65870..>66140
                     /locus_tag="KLH32_000680"
     rRNA            65870..>66140
                     /locus_tag="KLH32_000680"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15658 a        16369 c        18228 g        15885 t
ORIGIN      
        1 ggtacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca
       61 ttatctgaac ataaaacact atcagtaagt tggagtcatt accggcgatg ggattgatat
      121 ttatctgcgg gaatttatat tatgtttctg caaaaatgtt taaactaaaa tttttattaa
      181 ggaaagagag gatgtatgat tttcagaaaa tatttatatt cgtaatataa ataaattacc
      241 atttgcttaa tttgtcaaat gctcttgtat gtaagacttc acgcaacgtc tccccatcag
      301 gaaacgccag ctccggtgca atctcaaaca gcggccacag cataaatcca cgattcttca
      361 tatcgtagtg cggaacggtc aggcgttcgg tatttatcac ttcattacca aacagcatga
      421 tatcgagatc cagcgtgcgt ggtccccagc gttcagcttt acgaacacga ccctgttgca
      481 gttctatgcg ctgtgtatgg ttgagtaact cttccggggc gggcgaagtt tcaagcgcca
      541 cggcggcgtt gaggtaatcg ggttgatctt gcggccccag cggtggggtg cggtaaaacg
      601 aagaaacggc aagaatgcgg ctttcaggga tatcgcctaa tgctttcagg gcagcattga
      661 cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact gtcatgtggt
      721 accctcacga cgcggtgcgc gtttgcgtgg acgacgagta cgacgacgcg gtgacggttc
      781 ttcatccagc tcgttaagca tccctttttg atctggtggt gcggaaacct ggaactcacc
      841 ccaccatttc accagacgct gcagttcagc attacgttca acttcagctc gcaaggccaa
      901 caggtcataa gccgcacgga acttaggatg ctccagcagt ttccatgcgc gtttaccctg
      961 acgacgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg tcagacgttt
     1021 cgggattgcc agtgaacggc aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc
     1081 gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca gcagtgggta
     1141 ccagaacatg gcggcaaaca agaacgccgg gttcacgcgc atatcgttat ggatacgcgt
     1201 atcggtattc ttcagcacct gttcaatgat ccgctccatc gggctgtcgc cattttccgt
     1261 gaagtagcgg gtaatggtcg ggaacagcgg ctggaacaga tgatattcac acaacagctt
     1321 ataggtttcg taaccgtagc ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc
     1381 cggtgggata tcgttcagca gggtagcgag gcgagggatc ggttctgcgg tttccgggct
     1441 gatgcgcata cccaatttgg cggcaaaacg taccgcgcgc agcatacgta ccggatcttc
     1501 acggtagcgc gtttccgggt taccaatcag acggataacg ccgtccttca gatccttcat
     1561 accaccaacg taatcacgga cggtaaaatc cgctacgctg taatacaggc tgttgatagt
     1621 gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat
     1681 gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg
     1741 gaaggtcgca acttcgataa tctccgggcc aaacattaca tgagccagac ggaaacggcg
     1801 acccaccagg cggcagttac ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt
     1861 tacgtcaaaa tctttcggct ttttgccaag taacaggtcg cgcacgccgc cgccaaccag
     1921 ccaggcttcg tatcccgctt tgttgagcct gtacattacc ttcagggcat tttcactgat
     1981 atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg gacgggcgac
     2041 tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat tagcgactcg
     2101 ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa atagcggcta
     2161 atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga ctcgggcacg
     2221 gcggtcagca tccagttttt gacggctgac tgaaggattt gctcgacgct gaaatcctgc
     2281 cagtgtgctt ctgcctgctg ccccagaaat tgaagtgccg cgattagtac cgggcgtgga
     2341 tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta
     2401 agcgccagcg gcagatgaat gtaatcaggc actttccagc caaaaagctg gtacagcggg
     2461 atttgcctta ctgttggttc aatcagatca gccccacgca ctatttctgt aacgccctgg
     2521 aaatgatcat caaccacaac agccaggttg taggcgaaca acccatcacg gcgatgaatg
     2581 ataaaatctt cccgtgccag tttttcgtcg gcgtgaataa tgcctcgcag caggtcagta
     2641 aattgcgtga ccggatgctg ctggcggata cgcactgcgg cgttgtctgg tccatgatgc
     2701 aacacccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag
     2761 gtgcagtaat aacttagtcc ttgttcatgt aaccaggcga gtgcttcacg ataggcgtcg
     2821 tgacgttgcg attgccagag aacatcgccg tcccagtgca gaccgtaatg ttccagctgg
     2881 cgcaggatag tttctgcggc accgggaact tcacgaggcg ggtcgatatc ttctatgcgt
     2941 accagccagc gaccttgccg ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga
     3001 gagccaaaat gaagctcgcc ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct
     3061 gtcatctctt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta aacgtgatgg
     3121 aacggctgta attagccagc catctgtttt tcgcgaattt cagccagcgt tttgcagtcg
     3181 atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc aacaccgcag
     3241 gattcgcagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc gatctttttg
     3301 atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc ttcctgggct
     3361 gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt aacggtgcga
     3421 tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa gtgcgccagc
     3481 tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac cccagcgatg
     3541 gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt gcttctcctt
     3601 aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa atagcagatg
     3661 cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat
     3721 ttgcgcacgc gaccagaatg taaattaacc agttacttac tttactacaa tgtaaccggc
     3781 agtgattttt taagagccat gccttcagca gaaatttccg ctttgtaagc cagaatttct
     3841 acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg
     3901 ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa aaataaccgc acgctggcct
     3961 tcagccgcta cgctcatcaa ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg
     4021 ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat atagcagtct
     4081 ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttcggagcc gtattttact
     4141 tcgcttttca gcgagctata gcctgacagt tctgaaattg attcattaag gatagcctct
     4201 ttcgtcaacc tgttagccca aagcgtattg acgcaaataa atgcgccgct ctggctttga
     4261 gttaattccc aggtgtgtgg gtatttccgt ttggtgttgt ctgaagtcga ataccagacg
     4321 gtatcgccag gcgttgcaca accggtcatc gcacccgtat tcgggcagtg tagcgttaat
     4381 tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc
     4441 gcgcgctgta gagggggaga aaattccatt gcgacaatcc ttgttattgc gttagcgccc
     4501 agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga
     4561 actccgtcac cgcataagac cagttaaaac ccggcggcgg gaatgtcacc gcctcgctgg
     4621 cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaaaa caaccgctgc
     4681 gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtgga ggctgacgca
     4741 tccctaacca caccacacgc gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga
     4801 aaccaggttg acgaatccgt ccggctaaaa gagaaagcgc cttctctttc tctgcgctca
     4861 cttcgcctaa aaatgccaga gtcaggtgca aattatcggc ggcgaccgga cgtcccgcct
     4921 caggtgggaa gtgtgtggcg cgccaatgga taatctgttc gcggatttct gcaggtaagt
     4981 cgatagcaaa gaacagacgt tgcggttcag acatgtgagg cactcggtta tgaattaccg
     5041 cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg tcgtcgttgc
     5101 ccgttgctgc cgtcttacct gaattactta ccgctctcga ttgtgcgccg caggtattat
     5161 taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc
     5221 ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg cgtaacgtcg
     5281 cgcaacggct ggcggagctg cttaacgaaa agccaggcga taccgttggc taccggatgc
     5341 gtgcgcaaaa ctgcgtcggg ctgaataccc gcctggaagt ggttaccgaa ggcgtgctga
     5401 cgcggatgat ccagcgtgac ccggaactga gcggtgttgg attggtgatc cttgatgagt
     5461 ttcatgagcg cagtttgcag gcggatttgg cgttggcgct gttactcgat gtgcaacaag
     5521 gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gctggacaat gaccgcttgc
     5581 agcaaatgct gccggaagcg cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac
     5641 gccgttattt accgctgccc gcgcatcagc gttttgacga agccgttgcg gtagccaccg
     5701 ccgaaatggt gcgtcaggaa agtggatcat tactgttatt tttacctggc gtcggagaaa
     5761 ttcagcgcgt gcaggaacaa ctggcttcgc gcatcggcag tgatgtggtg ctctgcccgc
     5821 tgtatggcgt gttgtcactg aacgatcagc gaaaagcgat cctcccggca ccgcaaggga
     5881 tgcgcaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatc gaaggtattc
     5941 gtctggtggt ggattgtgcc caggagcgtg tggcgcgctt tgatccgcgc acggggctta
     6001 cgcggttgat tactcaacgc gttagtcagg catccatgac gcagcgtgcc gggcgcgccg
     6061 ggcgtctgga gccgggtatc agcctgcatt taatcgccaa agaacaagca gaacgcgccg
     6121 cggcgcaaag tgagccggag atcttacaaa gcgatctttc cggtttgctg atggaattac
     6181 tgcaatgggg atgcagcgat ccggcgcaga tgagctggct ggatcaaccg ccaacggtga
     6241 atctactggc cgcgaaacgc ctgttacaaa tgctgggggc gctggagggt gaacggctta
     6301 gtgcgcaagg gcaaaaaatg gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg
     6361 ttaacgcaaa gagcgatgac gaagctgcta ccgcggcaaa aattgccgcc attctcgaag
     6421 agccgccacg gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct
     6481 ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca
     6541 gttcgcttat cgcgccgcta cttgccgggg cgtttgccga tcgcattgct catcgccgtg
     6601 ggcaagatgg acgctatcaa ctggcaaaca gcatgggagc gatgctcgat gccgacgacg
     6661 cgctaagccg ccacgaatgg ttgatcgcac cgttattatt gcagggcagc gcctcgccgg
     6721 atgcgcggat tttactggcg ctaccggtcg atattgatga attagtacaa cgctgtccgc
     6781 agctggtgca gcaatctgac accgtggaat gggatgacgc gcaaggtacg ctgaaagcct
     6841 ggcgtcggct acaaatcggt cagttgacgg tgaaagtgca gccgctggcg aaaccgtcag
     6901 aagacgagtt gcatcaggcg atgcttaatg gcatccgtga taaaggttta agcgtgctca
     6961 actggacggc ggaagcggaa cagctacgct tgcgtttgtt atgcgccgca aagtggttgc
     7021 cggaatatga ctggccagcg gttgatgatg aaagtttgtt ggcgacgctg gaaacgtggc
     7081 tgctgccaca tatgaccggc gtacattcac tacgcggcct gaaatcactc gacatttatc
     7141 aggcactacg cggattactt gattggggaa tgcagcaacg tctggatagt gaattgcctg
     7201 cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa gataacccgc
     7261 ccgcgctggc ggtgagaatg caggagatgt ttggtgaggc cactaatccg acgatcgccc
     7321 aggggcgcgt gccgctggtg ctgaagttgc tttcccctgc ccaaaggccg ctgcaaatca
     7381 cgcgtgattt gggggccttc tggaaagggg cgtatcgtga ggtgcaaaag gagatgaaag
     7441 ggcgttatcc caaacacgtc tggccggacg acccggcaaa cacggcaccg acgcgacgga
     7501 cgaaaaagta ttcgtaaggt tggttttctc cctctccctg tgggagaggg ccggggtgag
     7561 ggcatcagcg cgcacgttca ccctcaccct aaccctctcc ctcaagggag aggggaccga
     7621 tcgagcgcaa attttgagag atatcttctt ctgtcttgta acagaagaac agaaaatcgg
     7681 gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc gagccaattg
     7741 gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag
     7801 atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag
     7861 gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctactgc
     7921 taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa
     7981 aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa
     8041 tggtcaacct tgaaccagat atgaccatca gcaagaacga aatggtgaag ctgctggagg
     8101 caacacagta tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca
     8161 acagcattga gatgatccgc cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc
     8221 gtgcgcgtct gacctttgat ggtgatcatc tggcgaccat cgtcaatatg gagaacaacc
     8281 gtcagttcgg tttcttccgt cttgatccgc gtctgatcac catgatctcc tcgccaaacg
     8341 gtgagcagcg tttgtttgtg ccgcgcagtg gtttccctga tttgctggtg gatactttgc
     8401 tggcgacaga agaccgtcat ttttacgagc atgatggaat cagtctctac tccattgggc
     8461 gtgcggtgct ggcaaacctg accgcaggtc gcacggtgca gggtgcgagt acgctgacgc
     8521 aacagctggt gaaaaacctg ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg
     8581 aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt
     8641 atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg
     8701 caagcttgta ttactttggt cgcccggtag aagagctaag cctcgaccag caggcgctgt
     8761 tagtcggtat ggtgaaaggg gcgtccatct acaacccgtg gcgtaacccg aaactggcgc
     8821 tggagcgacg taatctggtg ctgcgtctgc tgcaacagca acagattatt gatcaagaac
     8881 tctatgacat gttgagtgcc cgtccgctgg gggttcagcc gcgtggtggg gtgatctctc
     8941 ctcagccagc ctttatgcag ctggtgcgtc aggagttgca ggcaaaactg ggcgataagg
     9001 taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg
     9061 cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc
     9121 ttgaaactgc gattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggtcggag
     9181 gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt
     9241 cccttgcaaa accagcgact tatctgacgg ctttaagcca gccgaaaatc tatcgcctga
     9301 acacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac
     9361 cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg gatgcgttga
     9421 cacgttcgat gaacgtgccg acggtaaatc tggggatggc gctggggctg cctgcggtta
     9481 cggagacctg gatcaaactg ggcgtaccga aagatcagtt gcatccggtt ccggcaatgc
     9541 tgctgggggc gttgaactta acgccaatcg aagtggcgca ggcattccag accatcgcca
     9601 gcggtggtaa ccgtgcaccg ctttctgcac tgcgttcggt aatcgcggaa gatggtaagg
     9661 tgctgtatca gagcttcccg caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga
     9721 cactatggac catgcagcag gtggtacaac gtggtacggg tcgtcagctt ggggcgaaat
     9781 acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt
     9841 ttgcaggcat tgacggcagc acggtgacca tcacctgggt aggccgtgat aacaaccagc
     9901 cgaccaaact gtatggagcc agcggggcaa tgtcgattta tcagcgttat ctggctaacc
     9961 agacgccaac gccgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact
    10021 acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc
    10081 cgcaatcgct gtgccagcag agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc
    10141 agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca
    10201 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
    10261 aataccggat ggcgagttgc catccggtaa aataacatcc catctaagat attaaccctt
    10321 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10381 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatt
    10441 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10501 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10561 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10621 aaagcgcatg ggggcctgct gcaactattg cggcgcggca gtcagctacc ggcactaaaa
    10681 ccgatacgcc gattcaaaaa gtgccacagt ctatttctgt tgtgaccgcc gaagagatgg
    10741 cgctgcatca gccgaagtcg gtaaaagaag cgcttagcta cacgccgggt gtctctgttg
    10801 gtacgcgtgg cgcatccaac acctatgacc acctgatcat tcgcggtttt gcggcagaag
    10861 gccaaagcca aaataactat ctgaatggcc tgaagttgca gggcaacttc tataacgatg
    10921 cggtcattga cccgtatatg ctggaacgcg ctgaaattat gcgtggcccg gtttccgtgc
    10981 tttacggtaa aagcagtcct ggcggtctgt tgaatatggt cagcaagcgt ccgaccaccg
    11041 aaccgctgaa agaagttcag tttaaagccg gtactgacag cctgttccag actggttttg
    11101 actttagcga tgcgttggat gatgacggtg tttactctta tcgcctgacc ggtcttgcgc
    11161 gttctgccaa tgcccagcag aaagggtcag aagagcagcg ttatgctatt gcaccggcgt
    11221 tcacctggcg tccggatgat aaaaccaatt tcaccttcct ttcttacttc cagaacgagc
    11281 cggaaaccgg ttattacggc tggttgccga aagagggaac cgttgagccg ctgccgaacg
    11341 gtaagcgtct gccgacagac tttaatgaag gggcgaagaa caacacctat tctcgtaatg
    11401 agaagatggt cggctacagc ttcgatcacg aatttaacga cacctttact gtgcgtcaga
    11461 acctgcgctt tgctgaaaac aaaacctcgc aaaacagcgt ttatggttac ggcgtctgct
    11521 ccgatccggc gaatgcttac agcaaacagt gtgcggcatt agcgccagcg gataaaggcc
    11581 attatctggc acgtaaatac gtcgttgatg atgagaagct gcaaaacttc tccgttgata
    11641 cccagttgca gagcaagttt gccactggcg atatcgacca caccctgctg accggtgtcg
    11701 actttatgcg tatgcgtaat gacatcaacg cctggtttgg ttacgacgac tctgtgccac
    11761 tgctcaatct gtacaatccg gtgaatcccg atttcgactt caatgccaaa gatccggcaa
    11821 actccggccc ttaccgcatt ctgaataaac agaaacaaac gggcgtttat gttcaggatc
    11881 aggcgcagtg ggataaagtg ctggtcaccc taggcggtcg ttatgactgg gcagatcaag
    11941 aatctcttaa ccgcgttgct gggacgaccg ataaacgtga tgacaaacag tttacctggc
    12001 gtggtggtgt taactacctg tttgataatg gtgtaacacc ttacttcagc tatagcgaat
    12061 cgtttgaacc ttcttcgcaa gttgggaagg atggtaatat tttcgcaccg tctaaaggta
    12121 agcagtatga agtcggcgtg aaatatgtac cggaagatcg tccgattgta gttactggtg
    12181 ccgtgtataa tctcactaaa accaacaacc tgatggcgga ccctgagggt tccttcttct
    12241 cggttgaagg tggcgagatc cgcgcacgtg gcgtagaaat cgaagcgaaa gcggcgctgt
    12301 cggcgagtgt taacgtagtc ggttcttata cttacaccga tgcggaatac accaccgata
    12361 ctacctataa aggcaatacg cctgcacagg tgccaaaaca catggcttcg ctgtgggctg
    12421 actatacctt ctttgacggt ccgctttcag gtctgacgct gggcaccggt ggtcgttata
    12481 ctggctccag ctatggtgat ccggctaact cctttaaagt gggaagttat acggtcgtgg
    12541 atgcgttagt gcgttatgat ctggcgcgag tcggcatggc gggctccaac gtggcgctgc
    12601 atgttaacaa cctgttcgat cgtgaatacg tcgccagctg ctttaacact tatggctgct
    12661 tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt tcgtttctaa tttctctttt
    12721 ggggcacgga tttccgtgcc catttcacaa gttggctgtt atgcaggaat acacgaatca
    12781 ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt
    12841 gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg
    12901 ttctggtaaa tccactctgc tcaaaatgct tggtcgtcat cagcctccgt cagaagggga
    12961 gattcttctt gatgcccagc cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt
    13021 ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg aactggtggc
    13081 gattggtcgt tacccgtggc atggcgcgct ggggcgcttt ggtgcggcag atcgcgaaaa
    13141 ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg gcgcatcggc tggtcgatag
    13201 tctctctggc ggcgaacgtc agcgggcgtg gatcgccatg ctggtggcgc aggatagccg
    13261 ttgtctgttg ctcgacgaac cgacttcagc gctggatatc gcccaccagg ttgatgtgct
    13321 ggcgctggtg caccgtttaa gtcaggaacg tggcctgacg gtcattgccg tgttgcacga
    13381 tatcaatatg gcggcacgct actgtgatta tctggtcgcc ctgcgcagtg gtgaaatgat
    13441 tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga tttatggcat
    13501 cccgatgggt attttgccgc atccggcggg tgctgcacct gtgagttttg tttattgatg
    13561 agcggcttac ctcttatttc gcgccgtcga ctgttaacgg cgatggcgct ttctccgttg
    13621 ttatggcaga tgaataccgc ccacgcggcg gctattgacc ccaatcgtat tgtggcgctg
    13681 gagtggttgc cggtggaatt actgctggcg ctcggcatcg tgccttacgg cgtggcggat
    13741 accatcaact atcgcctgtg ggtcagcgaa ccaccattgc cggactcagt gattgacgtc
    13801 ggtttgcgta cagaacctaa ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc
    13861 tggtcggcag gatatggccc ttcatcagaa atgctggcac gtattgcgcc gggtcgcgga
    13921 tttaacttca gtgacggcaa acatccgttg gcgatggcgc gtaaatcgct gacggaaatg
    13981 gcagatttac ttaacctgca aagcgcagcg gaaacgcatt tagcgcaata cgaagacttt
    14041 atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac gcccgttact gctgacgacg
    14101 cttatcgatc cgcgccatat gctggtcttc ggtccaaaca gcttgttcca ggaaattctc
    14161 gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg cagtaccgcc
    14221 gtcagtatcg atcgtctggc agcgtataaa gacgttgatg tgctctgttt tgatcacgac
    14281 aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat gccgtttgtc
    14341 cgtacagggc gctttcagcg cgtacccgca gtctggtttt atggtgcaac gctttcggca
    14401 atgcactttg tgcgtgttct ggataacgcc atcggaggta aagcgtgagt aaacgaattg
    14461 cgcttttccc ggcgttattg ctggcgctgt tagtgattgt cgctacggcg ctcacctgga
    14521 tgaacttctc acaggcgctg ccgcgcagtc agtgggcgca ggccgcctgg tcgccggata
    14581 ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg gcgatttcgc
    14641 tgctggtggg cgcgggcctg gggctggtgg gcgtgctgtt tcagcaagta ctgcgtaacc
    14701 cgctggcgga accgacgacg ttgggcgttg caacaggcgc gcaactgggg attaccgtca
    14761 ccacgctctg ggcgatccct agcgcgatgg cgagccagtt tgctgcgctg gcaggggctt
    14821 gcgttgttgg cttaattgtc tttggcgtcg cgtgggggaa acggctgtcg ccggtaacgc
    14881 tgattttcgc ggggttggta gtgagccttt attgcggcgc aatcaatcag ttactggtta
    14941 tcttccatca tgaccagttg caaagcatgt ttctgtggag cactggaacg ctgacgcaaa
    15001 ccgactgggg cggtgttgag cgtttatggc cgcagttgct gggcggtgtg atgctgacgt
    15061 tgctgctact tcgtccgtta accctgatgg ggcttgatga tggcgtggcg cgcaatctcg
    15121 ggctggcctt gtcgcttgcg cgtctggcgg cgctgtcgct ggcgattgtc atcagtgcgc
    15181 tgctggtgaa cgctgtgggg attatcggct ttatcgggtt gttcgcgccg ctgctggcaa
    15241 aaatgctggg ggcgcggcgt ctgctgccac gactgatgct ggcgtcgttg attggtgcgc
    15301 tgatcctctg gctttccgat caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt
    15361 ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg ctgccgcgtt
    15421 tacgcagcat tagcgcgccg gatatgaagg ttaacgatcg tgtcgcgact gaacgccaac
    15481 atgtgctggc gtttgccctc gcgggcggcg tgctgttgtt gatggctgtg gtggtggcgc
    15541 tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag cggggcgttg ctcgaggatt
    15601 taatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggcgtc atgctggcgg
    15661 tagcgggctg tattattcag cgactgaccg gcaacccgat ggcaagcccg gaagtgctgg
    15721 ggattagctc cggcgcggcg tttggtgtgg tgttaatgct gtttctggta ccgggtaatg
    15781 cctttggctg gttgttaccc gcaggcagtc tcggggcggc ggtgacgctg ttgatcatta
    15841 tgatcgccgc cggacgcggt ggattttccc cacaccgcat gttactggcg gggatggcgt
    15901 taagtaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg
    15961 cgcaagtgct gacctggatt tccggttcga cctacaacgc gaccgatgct caggtctggc
    16021 gcaccggaat tgtgatggtg attttgctgg cgattactcc gctgtgccgc cgctggctga
    16081 ccattttacc gctgggtggt gataccgcgc gggcggtagg gatggcgctg acgccgacgc
    16141 gaattgcgct gctgctgtta gcggcttgcc tgacggcgac cgcgacgatg actattggac
    16201 cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggc tttcgacgga
    16261 cgatgccaca catcgtaatt tcggcgctgg tgggtggttt actgctggtg ttcgctgact
    16321 ggtgtgggcg gatggtgctg tttccattcc agatcccggc ggggctgctg tcgaccttta
    16381 tcggtgcgcc atattttatc tatttgctga gaaagcagag ccgttaatag aatgcctcgc
    16441 ccggtgggcg aggcaaaatt atcacaactt cgcgaacacc cgacgtgcag catcgatggt
    16501 gttattgata tcttccatgc tgtgcgccac agacataaag cccgcttcaa acgctgacgg
    16561 tgccaggtaa acaccttcat ccagcatcat atggaagaaa cgcttaaaac gctcgacgtc
    16621 acaggccatc acatcctgat agcacgtcac ggactcggcg tcggtaaaga agataccgaa
    16681 cataccaccg acgtggttaa cgaccagcgg aattcccgct tcttctgccg cttccagcag
    16741 accttctgcc agacgtgttg tcagctcatc cagcgtttcg tgaatgcccg gctgcgcgac
    16801 ttcattcaga caggcgaaac ccgctgccat cgcaatcggg ttaccggaaa gcgtacccgc
    16861 ctgatagacc ggacctgtcg gggccagcgc atccattaca tcacgacgac caccgaatgc
    16921 gcctaccggc attccaccgc cgatgatttt tccgaggcag gttaaatctg gcactacggc
    16981 gaaataatcc tgtgcgccag ctagcgctac gcggaaaccg gtcatcactt catcgatgat
    17041 cagcaacgcg ccaaattcgt cgcacagcgc acgcagacct ggcaggaact ctggcagcgg
    17101 cggaacacag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa tctcttgcgg
    17161 gtattgctca aacgcggcgc gtacagaggc cagatcgtta taagtacagg ttaaggtatg
    17221 tttggcgaaa tctgccggaa cgcccggcga gtttggctgg cctaacgtga gtgcgccaga
    17281 accggctttc accagcaggc agtcagcgtg accgtggtaa caaccttcaa atttaatgat
    17341 tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg cctcggtgcc
    17401 ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca gttgcgccat
    17461 tttcacttcc atttcggtcg gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac
    17521 ggcattgcgg atcgccggat ggttatggcc cagcaccatc ggcccccagg aaccgacata
    17581 atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt tttcgataaa
    17641 cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacac cgccagggat
    17701 cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt cctgattcgt
    17761 agaaaaagtg aatggctgct attccatgtt attcataaca agttaaatac tcgtcaaaca
    17821 tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt tgtgcatttg
    17881 aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat
    17941 ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta gaaacaccgc
    18001 aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc gataaaaccc
    18061 cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgtcgggctg gctgcggttg
    18121 cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcgctg gtacatactg
    18181 ctgataatta tccgctactg ttaaccgtcg cttttctctg ttcggcggtg ctggcgatgt
    18241 tcggctactt tttggtgcgc aaatacgcgc ccgaagcagg gggatcgggg atcccggaaa
    18301 ttgaaggggc gctggaagat caacgtcctg ttcgctggtg gcgtgtattg ccggtgaaat
    18361 tctttggcgg gctggggaca ctcggcggag gcatggggct agggcgcgaa ggaccaaccg
    18421 tgcagatcgg cggcaatatc agtcgcatgg tgcttgatat tttccgcctg aaaggtgatg
    18481 aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg
    18541 cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt cgctatacgt
    18601 taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg taccggattt
    18661 ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg cttaatacgc
    18721 tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt tttaataaat
    18781 gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg cggcaatatt accaaatggg
    18841 tactgatggg gggtgcgatt ggcggtctgt gtggattgct ggggtttgtg gcaccagcaa
    18901 cgtcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc agcatgggaa
    18961 tgctggtgtt tatcttcgtc gctcgggtca ttaccacctt actctgcttc tcttccggcg
    19021 cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga accgctttcg
    19081 gaatggttgc cgttgagctg tttccgcaat atcacctgga ggcggggacg tttgctattg
    19141 ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg
    19201 ttctggagat gaccgataac taccagctca ttttgccaat gattattacc ggtcttggcg
    19261 caacactatt agcgcaattt accggcggga aacctctata ctcggcgatt cttgcgcgca
    19321 cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca gccagcgaga
    19381 atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt tagaatttgc
    19441 ccgattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg ccgctggagt
    19501 ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc
    19561 tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat ggtttcacct
    19621 ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt ggcctggtgg
    19681 ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactacacc gaaggactgg
    19741 aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt tgcggttctt
    19801 cctttagcat ctaatctgtt gttcatgtgc cggataaact atccggcaca tcttctctct
    19861 tcaacgccca ttctcatcca gcgcaaacgt cggtagctta agatgccaac gaatagccgc
    19921 cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca gtggtacgga
    19981 aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag ttgcgtagat
    20041 ttctgtacgt aaaatcatgg ggatttcgcg gaccagtaca tcacgaatga tcccgccgcc
    20101 aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg cattaaaggc
    20161 tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca ccggcaacat
    20221 ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc tggtgaccat
    20281 ggcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat ccagcgccat
    20341 gtcgcgaatt gttccgtcgc ctactgcggt aaccacgccc agtaccagaa caccaaaagg
    20401 gtccatacgc aattttccgg ctaacaaaac gccgtagatg gcaaataccg ctgtgccgac
    20461 tatatccagc caatagacga gcattgttca atccccactg agcacctgaa aaggtcaggc
    20521 actaatctac ctgtgaaagc gcattacaga gctgttgtgc tgcgaggata atacgtgggc
    20581 ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc tgttcacccc
    20641 agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaata gcctgtggcg
    20701 agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg tctttaaaga
    20761 tgttttctcc gccacaaact tcgagtacct ggttctgaat cgactctttt ccactggtaa
    20821 atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca gcatattgcg
    20881 ctttcaattg cacgtactga tccagcaggg attgcgcggc ttgttcggcc ttgtctggtt
    20941 gcggactcca gggggccagt tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat
    21001 cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc tcggcattac
    21061 ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc agattcatcc
    21121 cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc
    21181 tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg ggagaaagcg
    21241 tgatgacgcg cggcgcggcg ttgagccaca gtggcgcgag aaaagacagg gcgaccagcg
    21301 ccctgaacag tgatttagcc atgtgccagt ttctgcacca gtgactcaac catcaggctg
    21361 gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg ttgatcggcc
    21421 acgtcggaga tggcgcgtac gacaacaaac gggacgttga aattgtggca gacatgggcg
    21481 attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg gattttcgcc
    21541 agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta
    21601 agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc agctttaaag
    21661 cctgccggac agcctggtaa ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg
    21721 tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagcgttgg tgccaggcca
    21781 ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag cagagtggca
    21841 cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc ggttccattc
    21901 agttggccgg tatagatttc gcaaccgccg agactgatag tttgacggtt ttcgattttg
    21961 tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt catagattta
    22021 ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga agcgtatttc
    22081 tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact ggcatcgtcg
    22141 ttaccgttca ccgcagggcg ttaaaaccga acatgagatc ctgcggatct tcgagagcga
    22201 tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga cccaggtttt
    22261 tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca
    22321 ggtgggacgc tacatcgcca aagaaatttt aagccgtctg aaagagctta aattgctgga
    22381 agcatacggc ctggatgaac tgaccggccc ctttgaaagc attgttgaga tgtcatgcct
    22441 gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcagcga taaatgactg
    22501 gtttcgccaa cgtttgcacc cggaagatgc cgaaagccag cctctgactg acgatcgctg
    22561 cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg cttaacgagc tgcggcgcaa
    22621 gattcgtcag gacttatgtc attttgaggg gaatgcacaa ggcattcgtc tggtgcatac
    22681 attgatgcgg atgaatctca cttgggcaca ggttggcggt attttaaaat atacccgtcc
    22741 ggcgtggtgg cgtggcgaaa cgcctgagac acatcactat ttaatgaaaa agccgggtta
    22801 ttatctttct gaagaagcct atattgcccg cttgcgtaaa gaacttaatt tggcgcttta
    22861 cagtcgtttt ccattaacgt ggattatgga agctgccgac gacatctcct attgtgtggc
    22921 agaccttgaa gatgcggtag agaaaagaat atttaccgtt gagcagcttt atcatcattt
    22981 gcacgaagcg tggggccagc atgagaaagg ttcgctcttt tcgctggtgg ttgaaaatgc
    23041 ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt tttttatgta
    23101 tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat ttattgataa
    23161 tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg
    23221 cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa catgtgttta gccatccgga
    23281 tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag agatttatcg
    23341 tcctttatta agcctgtcgt tatcagactt tactgaactg gtagaaaaag aacgggtgaa
    23401 acgtttccct attgagtcgc gcttattcca caaactctcg acgcgccatc ggctggccta
    23461 tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat gggaatatta
    23521 ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct atgcgtggga
    23581 tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag acgaacaata
    23641 aatttttacc ttttgcagaa actttagttc ggaacttcgg gctataaaac gaatctgaag
    23701 aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa aaaccacatt
    23761 agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct ctgcaacggc
    23821 ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac cgatgctcga
    23881 aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc
    23941 gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct gccaggaagg
    24001 ttctccgttc cagagctctc cgttctgcca ggggggccag ggcggtaatg gcggcggcca
    24061 gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata aaggctatgt
    24121 cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc tgagcgatgg
    24181 tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg cgctgatcca
    24241 aatccagaac ccgaaaaaac tgaccgcaat taagatggcg gattctgatg cactgcgcgt
    24301 gggtgattac accgtagcga ttggtaaccc gtttggtctg ggcgagacgg taacttccgg
    24361 gattgtctct gcgctggggc gtagcggcct gaatgctgaa aactacgaaa acttcatcca
    24421 gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg ctggttaacc tgaacggcga
    24481 actgatcggt atcaacaccg cgatcctcgc accggacggc ggcaacatcg gtatcggttt
    24541 tgctatcccg agtaacatgg tgaaaaacct gacctcgcag atggtggaat acggccaggt
    24601 gaaacgcggt gagctgggta ttatggggac tgagctgaat tccgaactgg cgaaagcgat
    24661 gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt ctgcctaatt cctccgctgc
    24721 aaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc cgatcagcag
    24781 ctttgccgca ctgcgtgctc aggtgggtac tatgccggtg ggcagcaaac tgaccctggg
    24841 cttactgcgc gacggtaagc aggttaacgt gaacctggaa ctgcagcaga gcagccagaa
    24901 tcaggttgat tccagctcca tcttcaacgg cattgaaggc gctgagatga gcaacaaagg
    24961 caaagatcag ggcgtggtag tgaacaacgt gaagacgggc actccggctg cgcagatcgg
    25021 cctgaagaaa ggtgatgtga ttattggtgc gaaccagcag gcagtgaaaa acatcgctga
    25081 actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca ctgaacattc agcgcggcga
    25141 cagcaccatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa tacgggaagg
    25201 ggttctcctt acaatctgtg aacttcgcca caactccata catcttcatc atcctttggg
    25261 catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat aacggaggac
    25321 ttatggctgg ctggcatctt gataccaaaa tggcgcagga tatcgtggca cgtaccatgc
    25381 gcatcatcga taccaatatc aacgtaatgg atgcccgcgg gcgaattatc ggcagcggcg
    25441 atcgtgagcg tattggtgaa ttgcacgaag gtgcattgct ggtactttca cagggacgag
    25501 tcgtcgatat cgatgacgcg gtagcacgtc atctgcacgg tgtgcggcag gggattaatc
    25561 taccgttacg gctggaaggt gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga
    25621 atctgcgtaa atatggcgaa ctggtctgca tgacggctga aatgatgctg gaacagtcgc
    25681 ggttgatgca cttgttggcg caggatagcc gtttgcggga agaactggtg atgaacctga
    25741 ttcaggcaga ggagaatact cccgcacttc tgaatgggcg caacggctgg ggatcgatct
    25801 caatcaaccg cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg gcgtggacag
    25861 cgcaatggcg gagttacaac aactgcaaaa cgcgctgact acgcccgagc gtaataatct
    25921 ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg
    25981 gcgctgggat gcagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga
    26041 gtacggccag ctgcgttttc gcgtttcact gggcaactat tttaccggtc ctggcagtat
    26101 tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga
    26161 aagtcgctgc tatttttatc aggatctgat gttacctgtg ttactcgaca gtttgcgtgg
    26221 cgactggcag gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa
    26281 cggcttgctg cgacgaacgc tggcggcgtg gtttcgccac aatgtgcaac cgctggcaac
    26341 gtcaaaggcg ttgtttattc atcgtaatac cctggagtat cggcttaatc gtatatcgga
    26401 actgaccggg cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca
    26461 actggatgaa gagcggtaga ttatgcgtta aggttgaggc agcgctggct cattcccctc
    26521 agcccgaagg ggagagggga atgcccgagt taaccgtcaa ttcgggatta tttacgcgtt
    26581 agtttttcca gatcggcttc aatctcgctg atcttattgg ttacgactga ctccaggtga
    26641 cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg ctgacagatt
    26701 tgatcaagct catcaatgat ataccgtaga ttcgggctga tttcctggac ttctttataa
    26761 ccctgaccca caccatcagc gacgaccgtt ttacgctgac gcggatattt aaacttaacg
    26821 ctcttggcga aaaactcgcc tttgtctttc tggaaataga ttttcagaat atcgttgttg
    26881 gcttcctgcc ggaggctgta acgatcaatt tcttcaggat tggtaatacc cagacttttc
    26941 agattgtcgt acatagcgtt acctcaaaat gagtcagtaa attgtgctta ttttagcatt
    27001 tggcttggcc cgcccggctc gactgtgatc gcagaaagcc tttttcttcc agacttctat
    27061 catggcgtaa aaatcaaaaa attacctgct ttattgtggc gatattccat gggatggatt
    27121 agcacattta tcttcgtttt gcgaattaca tgacagtccg aaacaagatg aaaaagtatt
    27181 taaaaaaggg ggaggaagaa tgagtaggac gaattattct gagccagaaa gcatacgggc
    27241 agcatcatgc tgcccgtata tgtactctta atcgatggta cgcagcagtt cgttaatgcc
    27301 gactttgccg cgagttttcg cgtcaacttt cttaacgata accgcacagt agaggctgta
    27361 tttgccatct tttgacggca gattacctga aacaaccaca gaccccgccg gaacgcgacc
    27421 gtagtggatt tcgccggttt cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc
    27481 catagagatg acggaacctt cttcgacaat caccccttca accacttcag agcgtgcgcc
    27541 aatgaagcag ttatcttcaa taatggtcgg gttagcctgc agcggctcca gcacgccgcc
    27601 gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt
    27661 cgcccaggta tcaaccatgg tgccttcatc aacatatgcg ccgatgttaa cgtaagacgg
    27721 catcagcacg gtgttacggg caataaacgc accctgacgt accgccgctg gtggcacaac
    27781 gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg gcgaatttca tcggcacttt
    27841 gtcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac ggaaagagag
    27901 cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt tttccgctac
    27961 acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc gggtaacggt
    28021 gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg
    28081 taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt tatcgtttgg
    28141 attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat taagcgcacg
    28201 ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg
    28261 ggcaccatga agcgaaattc ccagatcggc aaaaattttc ccgactcgcg ccagcagtcc
    28321 aggttggtcg agggcgatca gttcgaggaa cgatttgcgg tcggtatggg tcggcaaaaa
    28381 cgttacttcg gtttcaacag taaaatggcg taatttggcg ggttggcgac ggggctgcgg
    28441 tggctgccag ctactttgcg tcagcacctg ctccagacca aaccgaataa cctcatggcg
    28501 atctgcggac agcgggctgc catcgggttc cagcacgata aaggtatcca tcgccatacc
    28561 gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc
    28621 acagacggcg gcaaacagat aggggcggtc cgggctccag ataaaaatct cggtgcctcc
    28681 acgcgtagcc tgcgggctaa gcaataccag cggtttgctt aaatcatgct gtaataaatg
    28741 gcgggcatgc caggccagtt gatttgggct atggcggaca aaatagttag cacgacagcg
    28801 tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta gtgccagtgc
    28861 ctggagttgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca ttccgcgtcg
    28921 tagctgcttt tcggtggcaa aatagagttc gcgcaacaga ctttgcttcc agctattcca
    28981 cagtgtttcg ttggtggcgc aaatgtcagc cactgtcagg cataccagat agcgcagacg
    29041 attttccgtt tgaacttctt cggcaaactg cttgatgact tcagggtcct gaatatcgcg
    29101 gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc agccaggcaa ccagctgtgt
    29161 ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact acatcctgag caccgagaat
    29221 ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag
    29281 ctcagttgac ggcaggcgcg gccagacgtc cacacacaac ggatggcgct ggcgcgtttc
    29341 ttcactggca aaactctcca gtttcagcat cacgcggata gtatgttcat ccaccgtgta
    29401 ggcgtggaac agatcaaact gcatctgccc gacgatatgc gaccattgcg gcatatacgc
    29461 gccgagcacg ctatggcgat gcattggcaa tagtccgcgc cgcaccgctc cggggtgacg
    29521 cagaatgctc aaaaacagtt ttcgtgcttg cggaatatta cacagcggtt gttgcagatg
    29581 gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa atgccggtga tcgcactgtt
    29641 gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa atagtgtttc
    29701 atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgagtg gacgtggttt
    29761 ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt tgcagcagca tctggttgag
    29821 ttcactgacg cggcgtgtaa cgcggaagta atccttcatc atccgctcga ccggttcgtt
    29881 gccttcacca ctgtaattca gacgctgagc gacgctaagt tggcgatcga ataacagacg
    29941 attatcgtaa cggctgacga ccagatgcag ggcaaagcga atacgccaca gtatatgcag
    30001 acattcgttt aattccgccc gctccgctga ggttaagaag ccaaacccga ccatttcatc
    30061 cagcgatgtt gcgccaaaat gacggcgggc cacccattgc agagtgtgaa tatcgcgcaa
    30121 gccgccaggg ctacttttga tgtctggttc aaggttgtag ctggtgccat ggtaacgctg
    30181 atggcgctgg ttctgttctt caactttcgc tgcgtagaac ttgtcggaag gccagaatcc
    30241 ttcgctaaaa atatgttttt gcagttcgag gaagagcgca acatcgccaa ttaataagcg
    30301 ggattcgatt aaattggtgg cgacggttaa atccgataac ccttccaaca tgcactcttc
    30361 aagcgtgcgc acactatgac cgacttccag ctttacatcc cagagcagcg ttaacagctc
    30421 gcccactttt tgcgcctgat cgtccgggag ctttttacgg cttaaaatca gtaaatcgat
    30481 gtcagaaagt ggatgcagct cgccacgacc gtagccaccg acggcaacca atgccaggtc
    30541 ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt ctatgaactc
    30601 ggtacgtgcc tcaatcaact gttctgcaga gatcccattg tcaaaggcat cacccagcca
    30661 acgctggaaa gtatcgatat gggcttttat cccacagacg gttaattcat cacggggcca
    30721 ggcgcatgga ttttgcggtt gaccgggcag ggtggagaga gcggtgtttg cgtactgttc
    30781 tggaagggta ttcattgtgc gccacccata agattaaatt atcgcattaa aaaagccggc
    30841 attcgccggc ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc
    30901 gtagcgtcaa aatttcgcag ccgttatcag tcaccacaat agtatgctca tattgtgcag
    30961 acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg atctcttttt
    31021 tacccgcgtt gaccattggc tcaatggtga acgtcatccc cggtttcagc acgacgttgg
    31081 tttcacggga gtcatagtgc agcacctgcg gttcttcatg gaagccgcga ccaataccgt
    31141 gtccgcaata ttcacgaacg acggagaagc cttctgcttc gacaaatttc tgaatcgccg
    31201 caccgatttc gcgcagatta atgcctggtt ttaccatgcg tagcgccagg tacaggcttt
    31261 cttgcgtgat gcggcacagg cgttcgccca tgatggtcgg cttaccgacg ataaacattt
    31321 tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta acgatatcgc
    31381 catctttcag cagcttagca tcgtccggga taccgtggca caccacttca ttaatagaga
    31441 tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat
    31501 taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc ggtttaacat
    31561 acggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc attttttcga
    31621 tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt cggtgatacc
    31681 gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga gaatcattct
    31741 gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcgac gtctgatgct
    31801 gtacacagcg ccaacaatta ttggtgtcca cgacgtattt gtggtataaa gcgcgccgga
    31861 cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact ttgtgtaaca
    31921 acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata tgggatacgt
    31981 ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt tccatgcgcg
    32041 acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac ccgaaaatga
    32101 agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag aaaactgtac
    32161 cgatgttcaa cgaagctctg gctgaactga acaagattgt ttctcgcaaa ggtaaaatcc
    32221 ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg
    32281 accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg aaaaccgttc
    32341 gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt actttcgaca
    32401 agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc
    32461 tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc gatgctgacc
    32521 acgaacacat tgctatcaaa gaagcaaaca acttgggtat tccggtattt gctatcgttg
    32581 ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac gacgcaatcc
    32641 gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg
    32701 atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc ttgataactc
    32761 ccccaaaata gttcgagttg cagaaaggcg gcaagctcga gaattcccgg gagcttacat
    32821 cagtaagtga ccgggatgag cgagcgaaga caacgcatct gcggcgcgaa atatgaaggg
    32881 ggagagccct tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct
    32941 ttttcacttt tatatctgtg tggtttaatg ccgggcagat cacatctccg aggattttag
    33001 aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat
    33061 gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa
    33121 catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg
    33181 cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac
    33241 tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc
    33301 tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag aagaacgtgt
    33361 tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg
    33421 cgacgttctg ggttcttatc agcacggtgc gcgtatcggc gttctggttg ctgctaaagg
    33481 cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt
    33541 catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga
    33601 tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat
    33661 gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa
    33721 aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca tccgtttcga
    33781 agtgggtgaa ggcatcgaga aagttgagac tgactttgca gcagaagttg ctgcgatgtc
    33841 caagcagtct taattatcaa aaaggagccg cctgagggcg gcttcttttt gtgcccatct
    33901 tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta
    33961 ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc
    34021 gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc
    34081 ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg
    34141 ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg
    34201 aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac
    34261 ggcttggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct gatgtccgct
    34321 attccattga atggcgtgtg cgatagctac agctgggcag aagctatcag cctgttgcgc
    34381 aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca
    34441 gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt
    34501 gacggcgtgt ttaccgctga tccggcgaaa gattcaaccg caaccatgta cgagcaactg
    34561 acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctg
    34621 gctcgtgacc ataaattacc gattcgtgtt ttcaatatga acaaaccggg tgcgctgcgc
    34681 cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata
    34741 aataagggta agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat
    34801 tgttgcaggc agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga
    34861 ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa tagcctaata atccagacga
    34921 ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc
    34981 tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat
    35041 gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc
    35101 acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg
    35161 acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac
    35221 gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcctc cgatctcggc
    35281 ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa
    35341 cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta
    35401 cgtaatgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc
    35461 agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc
    35521 aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt
    35581 gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt
    35641 attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc
    35701 gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc
    35761 gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg
    35821 ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca
    35881 ggggagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga
    35941 ggaggttgat caggtgatgg cagccattgt tggtgctgct gggctgttac ctacgcttgc
    36001 tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg
    36061 acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga
    36121 acataacgct atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga
    36181 ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga
    36241 gacgccattg cgcgatttgg caacaatgac gcccgatcaa gcttgccgtc atccgaactg
    36301 gtcgatggga cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata
    36361 cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc
    36421 gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg
    36481 ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc
    36541 tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta
    36601 tgagcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac
    36661 gacagcattg aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg
    36721 ctttacggat atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc
    36781 acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg agtgaagtcg ccagaaaaga
    36841 ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgcgct atttgttagc
    36901 gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc
    36961 aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg
    37021 taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc acttagcgaa
    37081 aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg
    37141 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    37201 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt
    37261 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    37321 gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc
    37381 gataccagtc gttttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca
    37441 gccggaaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    37501 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    37561 atagatgaag agatgttaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    37621 gtaattagga ctgggggaga gcatcgcatt agtaactttt tgctttggca aattgcctat
    37681 gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    37741 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    37801 acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    37861 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg
    37921 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    37981 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    38041 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgttg
    38101 ggttggtgga ttgtcgcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    38161 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    38221 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    38281 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    38341 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    38401 ataggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    38461 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    38521 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    38581 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    38641 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    38701 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat
    38761 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    38821 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    38881 tcccgttggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    38941 tccgccacca tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag
    39001 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    39061 tgcctggcgt acgtccggtg gttggcgaaa tagcagccaa ttcgatagct gcggaagcac
    39121 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    39181 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    39241 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    39301 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggccg caaattgaac
    39361 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    39421 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    39481 tccgggataa cccgggtaaa tccttagcgt tagaaatcga aaggcagggg agtcccttgt
    39541 ctttgacatt aatcccgggg agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    39601 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    39661 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    39721 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    39781 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    39841 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    39901 ggcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    39961 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    40021 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    40081 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    40141 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    40201 cctcagtatg ccggtgcgta caggcgacac ggttaatgat gaagatatca gtaataccat
    40261 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    40321 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    40381 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    40441 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    40501 cgtcggtaaa tatagcgcca gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    40561 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    40621 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    40681 gccgtggtgg aatgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgatct
    40741 tgaaaccctg cgcagctact atctggaccg cggttatgcc cgtttcaaca tcgactctac
    40801 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg
    40861 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    40921 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacta aagtgaccaa
    40981 aatggaagat gacattaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    41041 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgt
    41101 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    41161 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ttggggagcg atctggtcga
    41221 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    41281 acgtgttccg ggtagcccgg accaggttga tgtagtctac aaggtgaaag aacgtaacac
    41341 tggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    41401 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    41461 cgattaccag acctatgctg aactgtcggt aaccaacccg tacttcaccg tagatggcgt
    41521 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    41581 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatta acgaatataa
    41641 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    41701 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    41761 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    41821 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    41881 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    41941 cgatcacaaa tgggttgttc tgggtcgtac ccgctggggt tatggtgatg gtttaggcgg
    42001 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tgcgtggctt
    42061 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    42121 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    42181 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    42241 gtttattagc gataagtatg ctaactcggt tcgtacttca ttcttctggg atatgggtac
    42301 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    42361 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    42421 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    42481 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    42541 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    42601 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagctgca ggtcttggtt
    42661 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    42721 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    42781 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaaactgc
    42841 agtccatgaa agcgggcagc gatcgcacca aactggaaaa agacgtgatg gctcagcgcc
    42901 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    42961 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    43021 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    43081 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    43141 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    43201 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    43261 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    43321 cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    43381 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    43441 cggcgaagct gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg
    43501 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    43561 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    43621 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    43681 gtggtaactg ggtgaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    43741 tcggtgcctg cacaaccatc gatcgcggcg cgctggatga cactgttatt ggcaatggcg
    43801 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    43861 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    43921 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    43981 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    44041 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    44101 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    44161 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    44221 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    44281 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    44341 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    44401 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    44461 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    44521 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    44581 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    44641 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    44701 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    44761 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg
    44821 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    44881 taatgagatt tatcagtttg cctccatcgg cgaagttaac caggatttga aatatgctgg
    44941 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    45001 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    45061 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    45121 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    45181 ccatcagttc tgcatcattg gtgcgcatgt gatggttggc ggctgctccg gtgtggcgca
    45241 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    45301 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    45361 taagctaatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    45421 ggcggaaaca tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg
    45481 tctgattcgt taatgactga ccagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    45541 tccggcgata tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    45601 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    45661 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    45721 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    45781 attgatgcgc ccgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    45841 accattcatt acgtcagtcc atccgtctgg gcgtggcgac agaaacgcgt tttcaaaata
    45901 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    45961 tacaatgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc gttagatcca
    46021 gataaaaatg gtgcccgtga tgtgctgggg atcccttacg atgcccactg tctggcattg
    46081 ttgccgggca gccgtggtgc agaagtcgaa atgcttagtg ccgatttctt gaaaactgcc
    46141 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccgctggt gaatgccaaa
    46201 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacctgtc ggttcatttg
    46261 ctggatggaa tgggccgtga ggcgatggtc gccagcgatg cggcactact ggcgtcgggg
    46321 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    46381 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    46441 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    46501 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa ccagccacgc gatgcacgat
    46561 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    46621 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    46681 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    46741 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    46801 tggcgctcta tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    46861 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    46921 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    46981 taccgatgcc tgcgatggct gtggtgaaag gggatagccg cgtaccagaa atcagtgccg
    47041 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    47101 cgcaatatgg ttttgcccaa cataaagggt atccaaccgc ttttcatctg gaaaaactgg
    47161 ctgaatatgg cgcgactgaa catcatcgtc gcagctttgg gcctgtcaaa cgcgcactgg
    47221 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    47281 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ttggccaaaa
    47341 ccgcaccgct ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    47401 tcaccaacct ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    47461 ctatcgtcgg ggcagatttt aatgtccagt gcgacctgct gggtgatgag ttaacccacc
    47521 tgacggtact ggcggcgaac aataccggct atcagaatct ggcgttgctg atctcaaaag
    47581 cgtatcagcg cgggtacggt gccgccgggc cgatcatcga tcgcgactgg cttatcgaat
    47641 taaacgaagg gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt
    47701 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    47761 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    47821 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    47881 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    47941 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    48001 aggagatgtg tgagctgttt gccgacatcc cagaagccct tgccaacacc gttgagatcg
    48061 ccaaacgctg taacgtaact atgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    48121 gggacatgag caccgaagat tatctggtca agcgtgcaaa agaaggcctg gaagagcgtc
    48181 tggcattttt attccctgac gaggaagaac gtcttaaacg ccgcccggaa tatgacgaac
    48241 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    48301 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    48361 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    48421 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    48481 ttgacttctg tatggagaaa cgcgatcagg ttatcgaaca cgtggcggac atgtacggtc
    48541 gtgatgcggt atcgcagatt atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg
    48601 acgtaggccg cgtgctgggg catccgtacg gctttgtcga ccgtatctcg aaactgatcc
    48661 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    48721 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    48781 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    48841 attttgcgcc gctttattgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    48901 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    48961 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccaccgc
    49021 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    49081 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc
    49141 aacctgactg cttcgaagat atgatcgcac tggtggcact gttccgccct ggtccgttgc
    49201 agtcagggat ggtggataac tttatcgacc gtaaacatgg tcgtgaagag atctcctatc
    49261 cggacgtgca gtggcagcat gaaagcctga aatcggtact ggagccaacc tacggcatta
    49321 tcttgtatca ggaacaggtc atgcagattg cccaggtgct ttctggttat accctcggtg
    49381 gcgcggatat gctgcgccgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    49441 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa
    49501 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    49561 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    49621 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    49681 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    49741 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    49801 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    49861 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    49921 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    49981 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    50041 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    50101 ggccggagca ggtggtatta gatggggaac gtgaaacgtt aggcctgtac ctgaccggac
    50161 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    50221 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    50281 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    50341 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    50401 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    50461 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc
    50521 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    50581 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    50641 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    50701 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    50761 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    50821 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    50881 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatctcg
    50941 gtgcatggca gattgcgcaa ctggcacgcc atccgcagcg tccttatacc ctggattacg
    51001 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgtgcgtat gcagacgata
    51061 aagctatcgt cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc
    51121 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    51181 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca
    51241 cctttatcga caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg
    51301 aagccattgc acgcaacctg cgtgaaatgt ctcgccttgg cgtaccggta gtttgtacgg
    51361 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    51421 tgctgcaata cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    51481 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    51541 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    51601 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    51661 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    51721 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    51781 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    51841 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    51901 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaaatttat
    51961 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52021 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52081 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52141 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52201 taacattacg ccgccgttca cgaaagcctt gtttacctac gtcaaagagc ggaagtacac
    52261 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct
    52321 gttttatgat tttttcggcg ggaataccct taaggctgat gtctctattt cggtcaccga
    52381 gcttggttcg ttgcttgacc acaccgggcc acatctggaa gcggaagagt acatcgcgcg
    52441 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    52501 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca actgccataa
    52561 atcactggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    52621 taatgcgttg gggattctgg gggggatccc gcgccgtgaa tttactcgcg acagcatcga
    52681 agagaaagtc gctgccacta cgcaagcaca atggccggtt catgcggtga tcaccaactc
    52741 cacctatgat ggtttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    52801 gatccacttc gattccgcct gggtgccgta tactcatttt catccaatct accagggtaa
    52861 aagtggtatg agcggcgagc gtgttgcggg aaaagttttc ttcgaaacgc aatcgaccca
    52921 caaaatgctg gcggcgttat cgcaggcttc actgatccac attaaaggcg agtatgacga
    52981 agaggcgttt aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt
    53041 tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa
    53101 ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc cagcggctgc gggaagagtc
    53161 tgacggttgg tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgcaggcc
    53221 cgttgcgcct ggcgaacaat ggcacggctt taacgatgcg gatgccgatc atatgtttct
    53281 cgatccggtt aaagtcacta ttttgacacc ggggatggac gaacagggca atatgagtga
    53341 ggaggggatc ccggcggcgc tggtagcaaa attcctcgac gaacgtggga tcgtagtaga
    53401 gaaaaccggc ccttataacc tgctgtttct ctttagtatt ggcatcgata aaaccaaagc
    53461 aatgggatta ttgcgtgggt tgacggaatt taagcgctct tacgatctca acctgcggat
    53521 caaaaatatg ctgcccgatc tctatgctga agatcccgat ttctaccaca atatgcgtat
    53581 tcaggatctg gcgcaaggga tccataagct gattcgtaaa cacgatcttt ccggtttgat
    53641 gttgcgggca ttcgatactt tgccggaaat gatcatgacg ccacatcagg catggcagag
    53701 acagattaaa ggtgaagtag aaaccattgc gctggaacaa ctggtcggta gagtatcggc
    53761 aaatatgatc ctgccttatc caccgggcgt accgctgttg atgccaggag aaatgctgac
    53821 caaagagagc cgcacggtac tcgattttct actgatgctt tgttccgtag ggcaacatta
    53881 ccccggtttt gaaacggata ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt
    53941 acgagtccta aaaatggcgg gataacttgc cagagcggct tccgggcgag taacgtgctg
    54001 ttaacaaata aaggagacgt tatgctgggt ttaaaacagg ttcaccatat tgcgattatt
    54061 gcgacggatt atgcggtgag caaagctttc tactgcgata ttcttggttt cacgctgcaa
    54121 agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg atttggcgct taatgggcaa
    54181 tatgtgattg agcttttctc ttttccgttc ccgccggagc gccccagccg accggaagct
    54241 tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg atgcggcagt ggcgcacctt
    54301 gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc catacacgca aaaacgcttc
    54361 accttcttta acgatccgga cgggctgccg ttggaactgt atgagcagta aggcttgtca
    54421 tcgccgcatt tgcccggtaa cgtgccgggc attgctactg taaaatcgca ccatcatgac
    54481 actcacgctc aatagacaac ttctcacctc acgccagatt ctggtggcct ttagcggcgg
    54541 gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg cggacggaaa atccgggcgt
    54601 cactctgcgc gctattcatg tgcatcacgg tttaagtgcc aatgccgatg cctgggttac
    54661 gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg gtggtcgaac gcgtacaact
    54721 tgcgcaagag gggctgggga ttgaggccca ggcgcggcag gcacgttatc aggcatttgc
    54781 ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa catctcgacg atcaatgtga
    54841 aacctttctg ctggcgctaa aacgcggtag cggccctgcc gggctttcgg ctatggcgga
    54901 agtttcggag tttgctggaa cacggcttat ccgcccgttg cttgcccgca cgcgggggga
    54961 actggagcag tgggcattgg cgcatggttt acgctggatt gaagacgaaa gtaatcagga
    55021 cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg ccgttattgc agcagcgttg
    55081 gccgcatttt gcggaagcaa cggcccgcag cgccacactt tgtgctgaac aagagagtct
    55141 gttggatgaa ctgctggcgg atgatttagc acactgccaa acgtcgcagg ggacgctgca
    55201 gattgcgcca atgctggcga tgagtgatgc ccgtcgcgcg gcgattattc gccgctggct
    55261 ggcagggcag aatgcaccga tgccttcccg cgacgcgctg gtgcgcatct ggcaggaagt
    55321 ggcgctggcg cgggaagatg cttccccctg tttacgtttg ggcgcctttg aaatccgccg
    55381 ctatcagtcg caactgtggt ggattaaatc cgtcaccggg caaagcgaaa ccattgtgct
    55441 gtggcagacg tggctacaac cgctggaatt accggcgggg ctgggaaccg tacagcttac
    55501 tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc
    55561 gccaggattg ctgcatattg tcgggcgcaa cggcggacgt aagctgaaga aaatctggca
    55621 agagttaggc gtgccgccgt ggctacgtga caccacgcca ctgctgtttt atggagaaac
    55681 gctgattgcg gcggcagggg tatttgtgac gcaagaaggt gtggctgaag gtgagaatgg
    55741 cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa gccggataag acgcatcaaa
    55801 cgtcgcatcc ggcgaaagtc aatcaggatt cgcttaccac caccgtaccg atttcagggt
    55861 ggctaaagct ggtaatttta tccagacgca gctcgcgggt ttcgccagcg gcctcgacga
    55921 ccagatactc cacatttttg cgggagacta aatcactggc ttttgcctgc aatttttcgc
    55981 catctttcag ctcaagtgtc agcattaaat gatgctggca ggcgagctca agattatcgt
    56041 aatcatcaca attaattggt tgatacgtat cattcattga cataatcgct caccagtaag
    56101 tttgccgcag cgtatgctgc tttttccctg acagcctcag aaagggcgtc gtcggcagcc
    56161 atttcattca gcactttcaa aacgcagccc agcgcgtccg gaacgtatcc taagtctccg
    56221 ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat atttttccac atgccctcct
    56281 gtcagcactc tgacttaacc gtggatgcaa gtctaagcct acgaagataa actctgtttc
    56341 gcaaggtgac tataccacac tcatttctgc aatatcagcg ccgcaactgc acgtattccg
    56401 ttacaatggc ctcctgattc gaaaggagtt ttcttatggc gcttaaagcg acaatttata
    56461 aagcgacggt taatgtggcc gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc
    56521 tggcgcgcca cccttcagaa acccaggagc gtatgatgct gcgcttgctg gcgtggctga
    56581 aatatgctga tgaacgtctg caatttaccc gtggtttgtg tgccgatgat gagccggaag
    56641 cgtggctgcg taacgatcac ctgggcattg atttgtggat tgagctgggg ctgccggatg
    56701 agcgacgaat taagaaagcc tgcacccagg ccgcagaagt ggcgctgttt acctataata
    56761 gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa atgtgtgcag tttgccaatc
    56821 tttccgtctg gtatctggac gatgaacaac tggcgaaagt aagcgccttt gccgatcgta
    56881 ccatgacgct gcaggcaacg attcaggatg gtgttatctg gttatcggat gataagaata
    56941 atctggaagt taacttaacc gcctggcaac aaccttcatg attgtgattt cccgacatgt
    57001 tgctattccc gatggtgagc ttgagatcac cgccattcgt gcgcagggcg ctggcgggca
    57061 gcatgttaat aagacctcaa cggctattca tctgcgtttt gacattcggg cgtccagcct
    57121 gccagagtat tacaaagagc gtctgctcgc cgccagccat catttgatca gcagtgatgg
    57181 cgtgattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg aagccgctct
    57241 ggcccggctg gtggcagtga ttaaagattt aacaaaagaa caaaaagccc gacgacccac
    57301 gcggcccacc cgtgcatcga aagagcgcag gctggcatcg aaagcacaaa aatcaagcgt
    57361 gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa aaagaaggaa tggatggtga
    57421 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
    57481 atcgggccga agtcgatacg ctttctccgg cgcaggctgc cgaactgaaa ccgatgccgc
    57541 aaagttggcg cggcgtgcta ccgtgtgccg attgcgaagg aatcgaaatc tctctgttcc
    57601 tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct cggtgctcgt gaagaacctt
    57661 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgaca
    57721 gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg
    57781 aaggcaatcc aattgaatcg cagttcaact atacgctgga accggcacaa tccagtttac
    57841 ctatgacgcc gatgaccctg cgaggcatgt atttttatat ggctgatgcg gcgaccttca
    57901 ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa cgcagagctg gagcgtagct
    57961 acctggctgc gcgcggtcac agtgaaaaaa cggtgttact gtcagtagaa ggtcacttta
    58021 cgcttgaggc taatccggat actggtgtgc caaccaaagt attagcgccc gatacggcgg
    58081 gtaaatttta cccgaataag gattgcagta gtttggggca gtaaacctgc ttaaggcaga
    58141 aacaaacgca aaattgcctg atgcgctgcg cttatcaggc ctacagcata taatgcaaca
    58201 ttttgaatta gcacgatttt gtaggccgga taaggcgttt acgccgcatc cggcattttg
    58261 tgcacgaggc tggctttatc actgagtcag cccaacaaag cgacccgcct tgatataaat
    58321 cccaggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg cagagacatc
    58381 accaatacgc atatgcatca gatcggcagg gctaatccag cccgattgcg ccagtgtaag
    58441 tggatgcccg gatttggcaa atgcatcagt gacaaattcc gaacaaaacc acgacttttt
    58501 gtctccttcg cacacactgc ttaactgcgc tttcgccagg tcgctgacgc actgttggcg
    58561 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
    58621 ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa acgcggtgat
    58681 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
    58741 cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
    58801 attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
    58861 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtgc
    58921 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
    58981 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
    59041 acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct ttggtttatc
    59101 cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc tggcatcagc
    59161 ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac tgtttctcgc
    59221 cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa
    59281 tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc
    59341 ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt ttctcagcaa
    59401 gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg
    59461 cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgcagcta
    59521 ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt
    59581 caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac
    59641 caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt ggatcgccag
    59701 ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc cagttgatac
    59761 cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg
    59821 tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggctt
    59881 taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca acctgcggca
    59941 gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg
    60001 ggaagctact gccttcaacc gctttaacca gcagagtctt aaccgttttc tcaatcggca
    60061 gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca
    60121 tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt
    60181 tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca
    60241 cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcctgt acggcgcgga
    60301 aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt
    60361 cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg
    60421 aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg atctgataga
    60481 agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca gtgataactt
    60541 cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt
    60601 ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg
    60661 gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt
    60721 ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga
    60781 tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct
    60841 tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta
    60901 gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa
    60961 atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc
    61021 gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg ttttgcccgt
    61081 ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag
    61141 cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa
    61201 actcaccgcc atctctgcag ctggcgcact ttgcgcatag ctggcactgg catcgggaag
    61261 cgattcggca aaggggagat acggtttgat atccactacc ggcgtaccat cgaccagatc
    61321 cagactgccg agcttcagaa tcacgctgcc tttatggcaa acaacctctt tcagctctac
    61381 cagcgacatg ccaattgggt tagggcggaa agtagagcgt gttgcgaaaa cccccattct
    61441 ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc cagccgcctt ccatcgtttg
    61501 atgaaagacg aaaaggatcc ataaatggct gaatgcttcc aggccgcgaa cggcgtcggc
    61561 ctggttgtag gaagcaatga gatgcagttc gccgttggcg ctttttacca gacctggctg
    61621 gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg
    61681 gaaactgctc atttcgccgt aatgttaagc gcagagccga tacatacagc ctgacgatag
    61741 cagcccggcg taccgctggt gacttcgcag ctatgcagta atacagcatt ggctttcatt
    61801 ttagaggcat tgatttgcat ctgcttacgt gcggttggaa tgctcggcgg agagtcctga
    61861 ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact
    61921 aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca
    61981 ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac
    62041 atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt
    62101 cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg
    62161 gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac agcctaaaaa ttaccagcct
    62221 ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcatc agactgataa
    62281 gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt tacgatcagg
    62341 tttacgtatg gggagtcttt atcttcgaca aagataccgt ctttcgccgg agtcaggcca
    62401 atctggctgg cataggtggt attgataact gccagagcga tttgcgcgtc gtccagagaa
    62461 cgcggcagtt gcggtgcttc cagttcaaca attttcagat tttttgggtt ctcaacaaca
    62521 tcaagaacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc
    62581 agcagcagca gtgaacgacc aagattagtt ggatcgtttg gcacggcaac ctgcgaacca
    62641 tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaaa
    62701 gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt
    62761 ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca
    62821 tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc
    62881 gcgacttctg ctacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga
    62941 tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc
    63001 gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt
    63061 gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa
    63121 accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag
    63181 ccatactgat agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa
    63241 taaccgacca gggtaatcag ggtgatagtt gccgcattca ccagacccgg caacgcttcc
    63301 ggtaacagca ctttacagac gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca
    63361 attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat aaacggtgct
    63421 gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg
    63481 cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc
    63541 acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc cggacgcgtg
    63601 acataaagca gaacgccaac cggcagacca atcacaaacc caaaaaatcc ggatacgaag
    63661 gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata
    63721 acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc
    63781 ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat ttaacgccac cggcgtagtc
    63841 catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag
    63901 tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtaaa
    63961 tggctccgtt tgcagacgtt cctggtaatc ttccgggata tccagatgca gggtcgactg
    64021 aataaacttc tgcgccagcg gcgttttcgg atgcgagaac acttcactta ccgtgtcctg
    64081 ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca caaatacgct tcacaacgtc
    64141 catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttaatgt ctttcagcag
    64201 ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa
    64261 tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga
    64321 aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt
    64381 cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc tccagcggca gagccacgtt
    64441 gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg
    64501 gcgagctttg gtcaactcgg attctgacag cgtggtcaat tcctggccat cgaccagcac
    64561 gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc
    64621 gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt
    64681 caacgcctgg atggtgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat
    64741 cattgattat ttattatcgt cattaagtta gtcgtagcat ctcgaatgcc tgaaacgggc
    64801 aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc
    64861 gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcccatgcc ttattaagga
    64921 gctataaaag gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa
    64981 tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcgacg gtgttattga
    65041 cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg
    65101 cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg
    65161 gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc atcatccgca
    65221 ggggagtgtt gaagagtttc gccaggtctg cgattgccgc aaaccacatc cggggatgct
    65281 tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa
    65341 attagaagat atgcaggcag cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac
    65401 gggtaaacct attacacctg aagcagaaaa cgcggcagat tgggtgttaa atagcctggc
    65461 agacctgccg caagcgataa aaaagcagca aaaaccggcg caatgattaa aagatgaacg
    65521 gttgaaataa aaatgcattt ttccgcttgt cttcctgagc cgactcccta taatgcgcct
    65581 ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa
    65641 aatcctgaaa ttcagggttg actctgaaag aggaaagtgt aatatacgcc acctcgcgac
    65701 agtgagctga aagctgcgtc gcaactgctc tttaacaatt tatcagacaa tctgtgtggg
    65761 cactcgaaga tacggattct taacgtcgca agacgataaa tgaataccaa gtctcaagag
    65821 tgaacacgta attcattacg aagtttaatt ctttgagcat caaactttta aattgaagag
    65881 tttgatcatg gctcagattg aacgctggcg gcaggcctaa cacatgcaag tcgaacggta
    65941 acaggaagcg cttgctgctt cgctgacgag tggcggacgg gtgagtaatg tctgggaaac
    66001 tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcataa cgtcgcaaga
    66061 ccaaagaggg ggaccttcgg gcctcttgcc atcggatgtg cccagatggg attagcttgt
    66121 tggtggggta acggctcacc
//
LOCUS       DAEEDS010000010        65633 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEEDS010000010 DAEEDS010000000
VERSION     DAEEDS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65633
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.010"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..101)
                     /locus_tag="KLH32_000681"
     CDS             complement(<1..101)
                     /locus_tag="KLH32_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0137642.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCS"
     gene            291..734
                     /locus_tag="KLH32_000682"
     CDS             291..734
                     /locus_tag="KLH32_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0137643.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            970..1788
                     /gene="ybhA"
                     /locus_tag="KLH32_000683"
     CDS             970..1788
                     /gene="ybhA"
                     /locus_tag="KLH32_000683"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBE0137644.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1789..2847)
                     /gene="modC"
                     /locus_tag="KLH32_000684"
     CDS             complement(1789..2847)
                     /gene="modC"
                     /locus_tag="KLH32_000684"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBE0137645.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2850..3539)
                     /gene="modB"
                     /locus_tag="KLH32_000685"
     CDS             complement(2850..3539)
                     /gene="modB"
                     /locus_tag="KLH32_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBE0137646.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3539..4312)
                     /gene="modA"
                     /locus_tag="KLH32_000686"
     CDS             complement(3539..4312)
                     /gene="modA"
                     /locus_tag="KLH32_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0137647.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4479..4628)
                     /gene="acrZ"
                     /locus_tag="KLH32_000687"
     CDS             complement(4479..4628)
                     /gene="acrZ"
                     /locus_tag="KLH32_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBE0137648.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4757..5545
                     /gene="modE"
                     /locus_tag="KLH32_000688"
     CDS             4757..5545
                     /gene="modE"
                     /locus_tag="KLH32_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBE0137649.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5613..7085
                     /gene="modF"
                     /locus_tag="KLH32_000689"
     CDS             5613..7085
                     /gene="modF"
                     /locus_tag="KLH32_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBE0137650.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7346..8362
                     /gene="galE"
                     /locus_tag="KLH32_000690"
     CDS             7346..8362
                     /gene="galE"
                     /locus_tag="KLH32_000690"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBE0137651.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8372..9418
                     /gene="galT"
                     /locus_tag="KLH32_000691"
     CDS             8372..9418
                     /gene="galT"
                     /locus_tag="KLH32_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBE0137652.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9422..10570
                     /gene="galK"
                     /locus_tag="KLH32_000692"
     CDS             9422..10570
                     /gene="galK"
                     /locus_tag="KLH32_000692"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBE0137653.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10564..11604
                     /gene="galM"
                     /locus_tag="KLH32_000693"
     CDS             10564..11604
                     /gene="galM"
                     /locus_tag="KLH32_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBE0137654.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11807..12559
                     /gene="gpmA"
                     /locus_tag="KLH32_000694"
     CDS             11807..12559
                     /gene="gpmA"
                     /locus_tag="KLH32_000694"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBE0137655.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12726..13778)
                     /gene="aroG"
                     /locus_tag="KLH32_000695"
     CDS             complement(12726..13778)
                     /gene="aroG"
                     /locus_tag="KLH32_000695"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBE0137656.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            14094..14474
                     /locus_tag="KLH32_000696"
     CDS             14094..14474
                     /locus_tag="KLH32_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBE0137657.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14588..15529
                     /gene="zitB"
                     /locus_tag="KLH32_000697"
     CDS             14588..15529
                     /gene="zitB"
                     /locus_tag="KLH32_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBE0137658.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15526..16245)
                     /gene="pnuC"
                     /locus_tag="KLH32_000698"
     CDS             complement(15526..16245)
                     /gene="pnuC"
                     /locus_tag="KLH32_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBE0137659.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16283..17326)
                     /gene="nadA"
                     /locus_tag="KLH32_000699"
     CDS             complement(16283..17326)
                     /gene="nadA"
                     /locus_tag="KLH32_000699"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBE0137660.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17575..17650)
                     /locus_tag="KLH32_000700"
     tRNA            complement(17575..17650)
                     /locus_tag="KLH32_000700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17615..17617),aa:Lys,seq:ttt)
     gene            complement(17654..17729)
                     /locus_tag="KLH32_000701"
     tRNA            complement(17654..17729)
                     /locus_tag="KLH32_000701"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17694..17696),aa:Val,seq:tac)
     gene            complement(17781..17856)
                     /locus_tag="KLH32_000702"
     tRNA            complement(17781..17856)
                     /locus_tag="KLH32_000702"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17821..17823),aa:Lys,seq:ttt)
     gene            complement(17859..17934)
                     /locus_tag="KLH32_000703"
     tRNA            complement(17859..17934)
                     /locus_tag="KLH32_000703"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17899..17901),aa:Val,seq:tac)
     gene            complement(18071..18146)
                     /locus_tag="KLH32_000704"
     tRNA            complement(18071..18146)
                     /locus_tag="KLH32_000704"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18111..18113),aa:Lys,seq:ttt)
     gene            complement(18311..19102)
                     /gene="cpoB"
                     /locus_tag="KLH32_000705"
     CDS             complement(18311..19102)
                     /gene="cpoB"
                     /locus_tag="KLH32_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBE0137661.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19112..19633)
                     /gene="pal"
                     /locus_tag="KLH32_000706"
     CDS             complement(19112..19633)
                     /gene="pal"
                     /locus_tag="KLH32_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBE0137662.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19668..20960)
                     /gene="tolB"
                     /locus_tag="KLH32_000707"
     CDS             complement(19668..20960)
                     /gene="tolB"
                     /locus_tag="KLH32_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBE0137663.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21093..22334)
                     /gene="tolA"
                     /locus_tag="KLH32_000708"
     CDS             complement(21093..22334)
                     /gene="tolA"
                     /locus_tag="KLH32_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBE0137664.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22399..22827)
                     /gene="tolR"
                     /locus_tag="KLH32_000709"
     CDS             complement(22399..22827)
                     /gene="tolR"
                     /locus_tag="KLH32_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBE0137665.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22831..23523)
                     /gene="tolQ"
                     /locus_tag="KLH32_000710"
     CDS             complement(22831..23523)
                     /gene="tolQ"
                     /locus_tag="KLH32_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBE0137666.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFVALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23520..23924)
                     /gene="ybgC"
                     /locus_tag="KLH32_000711"
     CDS             complement(23520..23924)
                     /gene="ybgC"
                     /locus_tag="KLH32_000711"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE0137667.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(24074..24367)
                     /gene="ybgE"
                     /locus_tag="KLH32_000712"
     CDS             complement(24074..24367)
                     /gene="ybgE"
                     /locus_tag="KLH32_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBE0137668.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24367..24480)
                     /gene="cydX"
                     /locus_tag="KLH32_000713"
     CDS             complement(24367..24480)
                     /gene="cydX"
                     /locus_tag="KLH32_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBE0137669.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24495..25634)
                     /gene="cydB"
                     /locus_tag="KLH32_000714"
     CDS             complement(24495..25634)
                     /gene="cydB"
                     /locus_tag="KLH32_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE0137670.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25650..27218)
                     /gene="cydA"
                     /locus_tag="KLH32_000715"
     CDS             complement(25650..27218)
                     /gene="cydA"
                     /locus_tag="KLH32_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBE0137671.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(28067..30700)
                     /gene="mngB"
                     /locus_tag="KLH32_000716"
                     /pseudo
     CDS             complement(28067..30700)
                     /gene="mngB"
                     /locus_tag="KLH32_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30718..32634)
                     /gene="mngA"
                     /locus_tag="KLH32_000717"
     CDS             complement(30718..32634)
                     /gene="mngA"
                     /locus_tag="KLH32_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBE0137672.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32804..33526
                     /gene="mngR"
                     /locus_tag="KLH32_000718"
     CDS             32804..33526
                     /gene="mngR"
                     /locus_tag="KLH32_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBE0137673.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33629..34498)
                     /gene="sucD"
                     /locus_tag="KLH32_000719"
     CDS             complement(33629..34498)
                     /gene="sucD"
                     /locus_tag="KLH32_000719"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBE0137674.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34498..35664)
                     /gene="sucC"
                     /locus_tag="KLH32_000720"
     CDS             complement(34498..35664)
                     /gene="sucC"
                     /locus_tag="KLH32_000720"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBE0137675.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35758..36975)
                     /gene="odhB"
                     /locus_tag="KLH32_000721"
     CDS             complement(35758..36975)
                     /gene="odhB"
                     /locus_tag="KLH32_000721"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBE0137676.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36990..39791)
                     /gene="sucA"
                     /locus_tag="KLH32_000722"
     CDS             complement(36990..39791)
                     /gene="sucA"
                     /locus_tag="KLH32_000722"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBE0137677.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(40092..40808)
                     /gene="sdhB"
                     /locus_tag="KLH32_000723"
     CDS             complement(40092..40808)
                     /gene="sdhB"
                     /locus_tag="KLH32_000723"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBE0137678.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40824..42590)
                     /gene="sdhA"
                     /locus_tag="KLH32_000724"
     CDS             complement(40824..42590)
                     /gene="sdhA"
                     /locus_tag="KLH32_000724"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBE0137679.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42590..42937)
                     /gene="sdhD"
                     /locus_tag="KLH32_000725"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42590..42937)
                     /gene="sdhD"
                     /locus_tag="KLH32_000725"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBE0137680.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42931..43335)
                     /gene="sdhC"
                     /locus_tag="KLH32_000726"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42931..43335)
                     /gene="sdhC"
                     /locus_tag="KLH32_000726"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE0137681.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43716..43823
                     /locus_tag="KLH32_000727"
     CDS             43716..43823
                     /locus_tag="KLH32_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137682.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            44029..45312
                     /gene="gltA"
                     /locus_tag="KLH32_000728"
     CDS             44029..45312
                     /gene="gltA"
                     /locus_tag="KLH32_000728"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBE0137683.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45703..46269
                     /locus_tag="KLH32_000729"
     CDS             45703..46269
                     /locus_tag="KLH32_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0137684.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46320..48779
                     /locus_tag="KLH32_000730"
     CDS             46320..48779
                     /locus_tag="KLH32_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0137685.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48794..49525
                     /locus_tag="KLH32_000731"
     CDS             48794..49525
                     /locus_tag="KLH32_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBE0137686.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49522..50598
                     /locus_tag="KLH32_000732"
                     /pseudo
     CDS             49522..50598
                     /locus_tag="KLH32_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50750..51796
                     /locus_tag="KLH32_000733"
     CDS             50750..51796
                     /locus_tag="KLH32_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE0137687.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51793..52584)
                     /gene="nei"
                     /locus_tag="KLH32_000734"
     CDS             complement(51793..52584)
                     /gene="nei"
                     /locus_tag="KLH32_000734"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBE0137688.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52620..53354)
                     /gene="pxpA"
                     /locus_tag="KLH32_000735"
     CDS             complement(52620..53354)
                     /gene="pxpA"
                     /locus_tag="KLH32_000735"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBE0137689.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53344..54276)
                     /gene="pxpC"
                     /locus_tag="KLH32_000736"
                     /pseudo
     CDS             complement(53344..54276)
                     /gene="pxpC"
                     /locus_tag="KLH32_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54270..54926)
                     /gene="pxpB"
                     /locus_tag="KLH32_000737"
     CDS             complement(54270..54926)
                     /gene="pxpB"
                     /locus_tag="KLH32_000737"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBE0137690.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54949..55692)
                     /gene="ybgI"
                     /locus_tag="KLH32_000738"
     CDS             complement(54949..55692)
                     /gene="ybgI"
                     /locus_tag="KLH32_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBE0137691.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55963..57444
                     /gene="dtpD"
                     /locus_tag="KLH32_000739"
     CDS             55963..57444
                     /gene="dtpD"
                     /locus_tag="KLH32_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBE0137692.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57594..59012)
                     /gene="phrB"
                     /locus_tag="KLH32_000740"
     CDS             complement(57594..59012)
                     /gene="phrB"
                     /locus_tag="KLH32_000740"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBE0137693.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(59009..59518)
                     /locus_tag="KLH32_000741"
     CDS             complement(59009..59518)
                     /locus_tag="KLH32_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBE0137694.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59647..59934
                     /locus_tag="KLH32_000742"
     CDS             59647..59934
                     /locus_tag="KLH32_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBE0137695.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59989..60369)
                     /locus_tag="KLH32_000743"
     CDS             complement(59989..60369)
                     /locus_tag="KLH32_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137696.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60372..65036)
                     /locus_tag="KLH32_000744"
                     /pseudo
     CDS             complement(60372..65036)
                     /locus_tag="KLH32_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(65055..65258)
                     /locus_tag="KLH32_000745"
                     /pseudo
     CDS             complement(65055..65258)
                     /locus_tag="KLH32_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15918 a        17775 c        16022 g        15918 t
ORIGIN      
        1 tgagagcaga gatagcgctg atgtccggca gtgcttttgc cgttacgcac cacgccttca
       61 gtagcggagc aggaaggaca tctgatggaa atggaagcca cgcaagcacc ttaaaatcac
      121 catcatacac taaatcagta agttggcagc attaccgtct tgccgaagaa gatccagctt
      181 ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt gacgagtatc
      241 tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc atggatcttg
      301 cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag gatataagag
      361 atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt tctctcccca
      421 ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg aaaaaaataa
      481 acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca gcacagttca
      541 ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca tttcatgaaa
      601 tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc actcaggcgc
      661 tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga gggagggaat
      721 ggaaagacat ttagaattaa tttcgaatga tttcgaaatg ccacttgtaa ttaattgatt
      781 ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca tttagcaact
      841 gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa acgcagaatt
      901 ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa ccttaatctg
      961 gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt attgaccccg
     1021 aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga agcaggctat
     1081 caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta tcaggcgctg
     1141 gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta tcatgcaaaa
     1201 accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat tgagatgctg
     1261 aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta tgagcacccg
     1321 accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga acagcgtccg
     1381 actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc cgtatggaag
     1441 ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca tgtcgaacat
     1501 gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc acacggcggc
     1561 aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc gatggaaaac
     1621 gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc tggtacaggc
     1681 gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt gattggtgat
     1741 aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat
     1801 cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct
     1861 ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac
     1921 ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt
     1981 ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc
     2041 agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc
     2101 ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc tactttgttg
     2161 ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa
     2221 ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc
     2281 cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata
     2341 aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag
     2401 caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga
     2461 caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc
     2521 gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg
     2581 cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg gcgtcaggca
     2641 gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg
     2701 gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa
     2761 gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat ggttgcccaa
     2821 cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg
     2881 gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt
     2941 ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc agaaggaatg
     3001 gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga
     3061 gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg
     3121 atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttccagtttg
     3181 acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg
     3241 gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac
     3301 agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc
     3361 acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac
     3421 gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc
     3481 agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt
     3541 acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat
     3601 cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca
     3661 cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctggcaactg
     3721 cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc gccagcgccc
     3781 cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg cccagttttt
     3841 gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca accgccaggc
     3901 gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa tctttctgct
     3961 cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga cgcgtagcag
     4021 tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca gaaataaaca
     4081 gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa gcgaaagaag
     4141 aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc attgcgttag
     4201 tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca ttgccagcaa
     4261 ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc
     4321 ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt tcccagcagt
     4381 tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc aggaagttga
     4441 gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg tccgggctgg
     4501 tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc
     4561 aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac
     4621 tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa
     4681 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
     4741 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
     4801 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
     4861 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
     4921 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
     4981 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
     5041 ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct
     5101 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
     5161 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
     5221 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
     5281 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
     5341 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
     5401 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
     5461 aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
     5521 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
     5581 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
     5641 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     5701 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     5761 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     5821 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     5881 tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt caggatgaag
     5941 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     6001 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     6061 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     6121 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     6181 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     6241 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     6301 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     6361 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     6421 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     6481 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     6541 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     6601 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     6661 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     6721 atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     6781 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     6841 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     6901 aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg ctgattagcg
     6961 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     7021 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     7081 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc ggccaacggt
     7141 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     7201 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     7261 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     7321 taccacaagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     7381 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     7441 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     7501 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     7561 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     7621 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     7681 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     7741 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     7801 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     7861 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     7921 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     7981 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     8041 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     8101 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     8161 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     8221 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     8281 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     8341 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatctcg ttgatcatcc
     8401 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     8461 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     8521 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     8581 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     8641 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     8701 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     8761 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     8821 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     8881 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
     8941 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     9001 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     9061 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     9121 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     9181 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     9241 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     9301 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     9361 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     9421 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     9481 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     9541 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     9601 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     9661 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     9721 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     9781 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     9841 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     9901 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     9961 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
    10021 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
    10081 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
    10141 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
    10201 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
    10261 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt ctcatgcctc
    10321 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
    10381 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
    10441 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
    10501 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
    10561 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
    10621 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
    10681 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
    10741 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc
    10801 atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
    10861 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
    10921 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
    10981 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
    11041 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
    11101 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
    11161 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
    11221 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
    11281 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
    11341 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
    11401 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
    11461 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
    11521 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
    11581 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
    11641 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca
    11701 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
    11761 ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg ctgtaactaa
    11821 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
    11881 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
    11941 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
    12001 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
    12061 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
    12121 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
    12181 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
    12241 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
    12301 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
    12361 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
    12421 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
    12481 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
    12541 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
    12601 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
    12661 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
    12721 aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag agcatcggta
    12781 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
    12841 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
    12901 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
    12961 ttggacgaat tagcatggct gaaatctatc atcacctgag ctggcagacc tgctttgttc
    13021 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
    13081 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
    13141 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
    13201 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
    13261 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
    13321 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
    13381 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
    13441 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
    13501 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgagtg
    13561 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
    13621 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc aaccgtattc
    13681 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
    13741 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gttgccatac
    13801 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
    13861 tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa
    13921 aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
    13981 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
    14041 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
    14101 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
    14161 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
    14221 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
    14281 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
    14341 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
    14401 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
    14461 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
    14521 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
    14581 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
    14641 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    14701 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    14761 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    14821 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    14881 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    14941 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    15001 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    15061 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    15121 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    15181 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    15241 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    15301 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    15361 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    15421 tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc ataggccaga
    15481 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    15541 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    15601 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    15661 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    15721 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    15781 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    15841 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    15901 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    15961 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    16021 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    16081 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    16141 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    16201 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    16261 taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc agcatacgat
    16321 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    16381 cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc cacggacaat
    16441 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    16501 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    16561 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    16621 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    16681 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    16741 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    16801 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    16861 aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg tagacgacga
    16921 cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg cagccgagat
    16981 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    17041 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    17101 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    17161 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    17221 aacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    17281 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    17341 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg gtgtaatttg
    17401 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    17461 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    17521 gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg
    17581 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    17641 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg
    17701 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
    17761 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    17821 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    17881 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgacact
    17941 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    18001 ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acgttttact accgcttcca
    18061 ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    18121 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    18181 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgca
    18241 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    18301 gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag
    18361 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    18421 gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc ggatagtttt
    18481 tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg tagtttaact
    18541 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    18601 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    18661 cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc gcagtaccag
    18721 catcagctgt cggcgtcgtt gatgccgtcg caccgctttg atcgctgctg gttgattgcg
    18781 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    18841 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    18901 attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    18961 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    19021 cctgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    19081 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    19141 tggagtatgc cgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    19201 tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg gcgttcgcac
    19261 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    19321 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc agcatttgag
    19381 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    19441 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    19501 catccatacc agtgccggca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    19561 tggaagaaca tgccgcaatt gccataacag gcagagcaat catcagccct ttcagcactt
    19621 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    19681 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    19741 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    19801 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    19861 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga gctgaccatt
    19921 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    19981 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accggcctgg
    20041 tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt attgttactg
    20101 cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta caggttcaga
    20161 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    20221 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    20281 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    20341 cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    20401 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac aacgtaggca
    20461 atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc atcactggcg
    20521 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    20581 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    20641 ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca tgcagctggt
    20701 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa cggattaaat
    20761 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    20821 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    20881 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    20941 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    21001 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    21061 gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    21121 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    21181 gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    21241 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    21301 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    21361 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    21421 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    21481 tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    21541 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
    21601 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt cttatcagct
    21661 gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc agcagcttca
    21721 gttgctgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    21781 agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    21841 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg ctgcttcttc cgcagcttta
    21901 gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa ctcggcctgt
    21961 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    22021 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    22081 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    22141 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    22201 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    22261 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    22321 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    22381 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    22441 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    22501 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    22561 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    22621 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    22681 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
    22741 caacagcacc agcagtacgt ccagcaacgg aacaatgttg atttcggact tgagatcgcg
    22801 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
    22861 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    22921 tttgtttacg cgctggttga gacggttata agccattacc gccggaattg cggcgaacag
    22981 accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    23041 ttgttttacc gccccgaggg cgacaaaggc gtgcatgatc ccccagaccg taccaaacag
    23101 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    23161 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    23221 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
    23281 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    23341 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag
    23401 aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    23461 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    23521 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    23581 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    23641 cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    23701 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    23761 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    23821 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    23881 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    23941 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    24001 tgagggcaag ttaagggcgc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    24061 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
    24121 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
    24181 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
    24241 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
    24301 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc gcgataatct
    24361 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
    24421 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
    24481 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
    24541 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
    24601 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
    24661 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
    24721 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
    24781 atacgtgcag tcaggatggt cagcagcagc agaaccacac ccagtgccgg aatagcccac
    24841 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
    24901 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
    24961 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
    25021 tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg cagataggtt
    25081 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
    25141 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
    25201 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
    25261 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
    25321 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
    25381 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
    25441 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
    25501 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
    25561 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
    25621 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
    25681 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac
    25741 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
    25801 aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag cacttcaccg
    25861 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
    25921 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
    25981 cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc acacgccacc
    26041 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
    26101 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
    26161 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
    26221 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
    26281 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
    26341 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
    26401 ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
    26461 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
    26521 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
    26581 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
    26641 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
    26701 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
    26761 ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga accgagcgcc
    26821 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
    26881 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
    26941 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
    27001 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
    27061 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
    27121 aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
    27181 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
    27241 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
    27301 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
    27361 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
    27421 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa
    27481 attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat gcccatttcc
    27541 aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg tttcaacaag
    27601 gtaactttag tgatatttat gtttaaaatg tgtgaattat aagactgaaa ttagtttcgg
    27661 ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt
    27721 tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc ataaatctcc
    27781 cccccgccga tattaacatt taattaactt ataattatat tataattaac tcacccgtgg
    27841 ttattatttt gttaaaaata aagccacata tacgtagctt gagagtgaag ataaacacaa
    27901 agttacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat attgaatttg
    27961 cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt
    28021 tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag gcaagccggt
    28081 aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa ccttgatttt
    28141 cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc tcgcgactga
    28201 aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt aaaatcactt
    28261 cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg ggcattttca
    28321 acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc gcatcccatg
    28381 ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga gctacaccag
    28441 cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc agaccacgta
    28501 gttgtgagtc cgggactggc attttaattc ccgaaggccg cctaggcctt aaaagcagat
    28561 cttctttccc cagtaagccc acgccgcgca gcaacgtaat ggcaaaggtt ttcttctctt
    28621 caccgatgac ttcaaattca cgtagccctt cgctaaagac agccatgccg ttacgccctt
    28681 cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct ttccagcctt
    28741 cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa ccaaactggg
    28801 tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga aggcgatgat
    28861 cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta tgactaagag
    28921 tgacaaccaa ctcgacatct actctgccag tggattgtct tgcgctgcgt tgttgcagat
    28981 tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc tcatgaataa
    29041 tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt gcgggtgaat
    29101 agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg tcatagcgca
    29161 caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca atttgccaga
    29221 aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg ccaggctgat
    29281 tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg atctgtatat
    29341 caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg attaggcctg
    29401 gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga tcgtctcgta
    29461 gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc cgcggccagg
    29521 gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg tcggcgattt
    29581 tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg aagcgggcga
    29641 cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg gcatgatttt
    29701 ttaagatctc tttccacatt ttctccagca agccgtggtg gtatccaaaa cccaacgtcc
    29761 tggccagcgt tgccagcggt tccagcagat taacaatctt attttcaata cgtgcgtggg
    29821 caactttgat atccatgcgc gtggaaccga tggtgcgatg cacgcgcata tatttgccat
    29881 caataaattc ccctttcagg gttgccagat tatctcgctg cgcttcgatc ttctcaaata
    29941 cctcttcaaa gcggctcatc acaaactttc gttgaggata aatttcgcgc agtttctcca
    30001 tcacttcgaa gatattttgc tgcaatggca tctgatcatg cccattcggc agcaaaatct
    30061 ctttggttac agacgctttt tccagcacgt caaaataact gtcgaggcgt ttgcgtaatc
    30121 cgttttcgtc cgcaggtaag tacttaccga tggcataacc caacggcatc acctgcgccg
    30181 tcacttcgct accgtcactg ctttgccaca aaaactcggt tttatccgta ccgtggtgct
    30241 cagaacatcc acgccagaac atggtgcagg taatgccaaa tccattgtag atatgcggca
    30301 gttgcccgga catgccaaag gaatcgggta aataacctat tttcatcggc tcgccaaacg
    30361 cgaggcagtc acgcattccg tacatcagat tacggacgat ggattccgca gaaacaatcg
    30421 tggtatcggt ctgggtatac taggggccga taatcaactt gccggcttct accagtttct
    30481 tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc ttcgaggatc gccgtttgcc
    30541 cgtcgagtac gtaatatttg tattcgttgt cctgttccag tcggcacagg atctcttcca
    30601 tattattgac cagcagaatg cgtgactctt cggtggtgaa ataccactcc cgatcccagt
    30661 gcatatgcgg ggtgatgtga acgcgagata ccgctttcat cgtcgtttcc tttttgttta
    30721 tggcattacg ccatcagtca gatagttgcc atgcttaacc gcgtgacgcc gccagatcag
    30781 gagaattgca gtcgagattg cagcccccac caatgccgca ccaaaccagc caattgccgc
    30841 cataacaccg cccgcgccat tatcatgaag taaaaagagc gagaaaatgc cagcaccggg
    30901 tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc gttaccatag agcccagcac
    30961 aaacgaacca ataacccgca gcggatcttc aatcgccatc gggatcgccc cttcggtaat
    31021 acctgccagc cctaacagcc aggtggattt cccggtctca atttcaaact ctttaaacag
    31081 gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc gaaaccattt tgacggaggc
    31141 gaaaatggca tacgggccgt aaacgccgtt cgccattgcg cccaggcaga atgcataagc
    31201 ggctttattc actggccctc caaggtcaaa ggaacacata aaaccgagaa tggcacccag
    31261 caacagcgcg ttacttcctg acagaccgtt cagccaggcg gtaagcgagt tattgatcca
    31321 ggcgacaggt tcccccacca caaacagcat cagactgccc gctcccaatg taccgagcac
    31381 cgggtagaga taaaaagtca ggaagccatt gaatttactg ctaagacgca agtgattttt
    31441 catccagcgt atcaagtaac cggctatcaa tccgccaacg accgcgccga gaaacccgga
    31501 gccgatcatg ttggcggcaa gtccagccgc aaagcctggc gctaacgccg gtttatctgc
    31561 cagcgaatag gcggtatagg ctgccagcac cggtaccatc aaaattccga gcagcccgcc
    31621 gcccagtttg cggtacatcc acagccagga gttttcttca ttaaacagat cttgtagccc
    31681 gaagatttgc gacagtaata ccgcgaccgc cagcaccgtc ccccccgcga caatcaacgg
    31741 tacggcaaaa gagatcccgc tcaacagtgc ctgtttcagc tccgttttga cgcttttcac
    31801 cggttgcgta tcttgttgta aggtacgcgt ctcatcgcca cgctcgaggg taagcgattg
    31861 ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca ggcacagaaa gcgcaggaat
    31921 gccgttaaaa cgctcactct ccttgatggc gacttcagcc gcaaaaatac aggcagttgc
    31981 actattgagt tgatccgccg ttaaacgccc ttcaataccg ttagcacctt gcttttcaac
    32041 gtagacattc acgccgagtt tgcgtccggc tttttccaga tactccgcag ccatgtaggt
    32101 gtgagcaata cccgccgggc aggccgttac gcaaacgata gttggcgcgt tggaaaaaga
    32161 ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag agcaattcat caggcgtcgt
    32221 cgccgactgt atacgcgccc gaatctcatc atccgccagg cgcgtggtca gcgctgtcag
    32281 cagttgcatg tgcgtagtac ccgcttcatt gaggggaatc gccagcaaca ccactaaatc
    32341 aaccgcttcc gggccatcca cgccttccca ctgaagcggc tcgctgagtg tggcgaccgc
    32401 aaacgccgct tcttttaccg cagcagtttt gccatgcggc acagccaacc cttcacttaa
    32461 ggcggtcggg ccaaggcttt cacggcgata cacttctttc agaaattgct cagtactgga
    32521 aattttcccc agagcagcaa gacgttgagt caacgcgtga atggcctctt cacggctggt
    32581 aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga gtcgtcaggt tcatcatgat
    32641 cctcacttga tcgtttttat agtcgcgcca gtgtgcccga caaaacaata ccattgcgta
    32701 atcaatttca cttgattgaa attatttgca ttttatttgt attgtttaca ggatgaattt
    32761 atcgggcata atggctaaat tcgtattaat gagatgctaa ataatgggac acaaaccctt
    32821 ataccgacag attgctgatc gcattcgtga gcaaattgcg cgtggcgagc ttaaacctgg
    32881 cgatgcgtta cctaccgaat cggcattaca aacagagttt ggcgtcagcc gggttacggt
    32941 gcgtcaggct ttgcgccagt tagtcgagca acagatcctc gaaagcattc agggcagcgg
    33001 gacttacgtc aaagaagagc gggtcaatta cgatattttt cagttaacca gttttgatga
    33061 aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtt ctgatatttg aagtgattcc
    33121 ggctgacgat tttcttcagc aacagctaca aatcaccgtg caggatcgtg tgtggcatgt
    33181 gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg gaagaaacct ggatgccgct
    33241 tgcgttgttc ccggatctca cctggcaggt catggaaaat tcgaaatatc actttattga
    33301 agaagtgaag aagatggtta tcgatcgtag cgaacaggaa atcattcctc tgatgccaac
    33361 cgaagagatg agccgtctgc ttaatatcag ccagacaaag ccgattcttg aaaaagtatc
    33421 gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc cgcaacgctt ttaataccga
    33481 tgactataaa tttaccctga tagcgcagcg aaaatcatcg cgataagcac aaaaaggcca
    33541 tcatacgatg gccaaccatg tcgaaaccga cagcccttcg ccattcctgt agagggaagc
    33601 ggcgagggct atttcttatt acagatattt atttcagaac agttttcagt gcttcaccga
    33661 tatccgccag gctgcgaacg gttttcacgc ctgcggcttc cagagcagcg aatttctcat
    33721 ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg gcccatacgt ttgcctttcg
    33781 gcgcagtcac accagcgatg taacccacaa ctggcttggt aacatgctct ttgatgtacg
    33841 ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat catcacgatc gcttcggtct
    33901 gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt agagccaggg atcgggtcac
    33961 cgccgatacc gacacaggtc gactgaccga aaccgtaatc cgtggtctgt ttaaccgctt
    34021 cataggtcag tgtaccggaa cgggaaacga tacccacttt acccggtttg tgaatgtgac
    34081 ccggctggat accaattttg cattcacccg gggtgataac gcctgggcag ttcgggccga
    34141 tcatacgaac gcctgcttcg tccagtttca ctttcacggt cagcatatcc agcgtcggga
    34201 tgccttcagt gatggtgata atcagtttga tgcctgcatc gatagcttcc agaatggagt
    34261 ctttgcagaa cggtgctggt acgtaaataa cagacgcgga agcgccagta gcagcaacag
    34321 cttcacgcac ggtgttgaac accggcaggc cgaggtgggt agtgccgcct ttacctggag
    34381 ttacgccacc aaccatttta gtgccgtatg caatggcctg ttctgagtgg aaagtcccct
    34441 ggctaccggt aaagccctgg cagataacct tggtgttttt atcaattaaa atggacatta
    34501 tttcccctcc actgcggcaa caacctgctg agctgcatcc gtcagacctt ttgctgcaat
    34561 aatattcagg ccgctgtcag ccagtttctt cgcgccgagt tcggcgttgt taccttccag
    34621 acgtaccacg accggtacgt taacacccac ttccgctacc gcgccgatga taccatcagc
    34681 gatcaggtcg caacgaacga taccgccgaa gatgttaacc agaacggctt tcactttgtc
    34741 gtcagagagg atgattttga acgcttcggt tacacgttct ttggttgcgc cgccgccaac
    34801 gtccaggaag ttagccggtt cgccgccgtg cagtttaact atgtccatcg tacccatcgc
    34861 cagacctgcg ccgttaacca tacaaccgat gttaccgtcc agcgcaacgt agttcagttc
    34921 ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac tggtcacgca tttcgcgcag
    34981 atcaggctgg cggaacaggg cgttgccatc agcgcccagt ttgccgtcga ggcaaatcag
    35041 atcgccctgt ttggtgatga ccagcgggtt gatttcgatc agcgccaggt cgcgctccag
    35101 gaaaatggtc gccaggccca tgaagatttt ggtgaactgc tgaaccagtt taccttccag
    35161 acccagtttg aacgccagct cgcgtccctg atacggcatc gggccagtca gcggatcaag
    35221 cgcaacttta tggatcaggt gcggagtttc ttccgccact ttttcgattt ccacgccgcc
    35281 ttcggtggag gccataaaga ccacgcgacg ggaactacgg tctacaacgg caccgagata
    35341 cagctcttta gcgatatcgg tcgccgcttc aaccagaatc tggttaaccg gttggccatt
    35401 ggcatctgtt tgatacgtta ccagacgctt gcccagccag ttttctgcaa aagcacggat
    35461 gtcttctttg ctgtttacaa ctttcacacc gcccgcttta ccgcggccac cagcgtgaac
    35521 ctgacatttc actacccacg gaccggcacc gatttttgaa gcggcttctt ctgcttcgcg
    35581 cggagtagta caggcataac ccaccggtgc tggtaagcca tagcgggcaa acagttgttt
    35641 tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct tcagtaatcg ttatctttta
    35701 aaccgtagac cgacacccta tccacgtcgg tctacagtgc aggtgaagct taaactacta
    35761 cacgtccagc agcagacgcg tcggctcttc cagtaactct ttgatcgtta ccaggaagcc
    35821 cacggattcg cgaccatcga tcagacggtg atcgtaggac agcgccaggt acatcatcgg
    35881 caggatctca acctgaccat tcaccgccat cggacgatct ttgatagcgt gcatacccag
    35941 aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc agggaaccga acacaccacc
    36001 gttggtgatg gtgaagttac caccggtcag atcttcaacc gtcagcttgc catcacggcc
    36061 tttgactgcc agctctttga ttttcttctc gatgtctgcc atgccgaggg tatcgacatc
    36121 acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc gccatgctga cgtcgaaata
    36181 gttgtggtaa accacgtcat cgccgtcgat agacgcgttc acttccgggt aacgtttcag
    36241 ggcttcaacc accgctttca cgtagaagga cataaagccc agacggatgc cgtggcgttt
    36301 ttcaaacgct tcaccgtact gcttacgcag atccataatc ggcttcatgt tgacttcgtt
    36361 gaacgtggtc agcatggcgg tggagttttt cgcttccagc agacgctctg ccacacgctt
    36421 acgcaggcga gtcatcggga cacgtttttc actacgtgca gccagagccg gttgcgccgc
    36481 cggagcagcc gctgccggag cagactcttt cgccggggct ttcgccagat gtttttccac
    36541 atcttcacga gtcagacgac cacccacacc ggtgccttta atggcgctgg cgtcgagatt
    36601 gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca tcgttgtttt gctcttccag
    36661 agacgcctgc tggcgttgcg ccggagtgga cgctttctct tcagatttgg cgctggtttc
    36721 tttaccggcg ctgttgcctt cacgcaggcg accaaggatc tgacgagacg ttaccgttgt
    36781 accttcatct tccagaaccg catccagaat gccgtctgct gatgccggta cttccagtac
    36841 cactttgtca gtttcgattt ctaccagcac ttcatcacgt acgactgcgt cgccgggttt
    36901 tttatgccag gttgcgacgg tggcatcggc tacggattca ggcaggtcag ggaccagaat
    36961 atctacgcta ctcattgtgt atcctttatt tattcgacgt tcagcgcgtc attaaccaga
    37021 tcttgttgct gtttctggtg aacggacata taccctaccg ccggagaggc ggaggccggg
    37081 cggcctgcat aacgcagaga agccccaaac ggaatcactt cacggaaatg atgctggctg
    37141 cagtaccatg cgccctggtt gagcggctct tcctggcacc agacaaaatc cttgacgtga
    37201 gcaaactgct gcaacacttc ctgcatcgct ttatgcggga acgggtagag ttgctcgata
    37261 cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct gttccagcag gtcgtaataa
    37321 accttaccag aacacatcac tacgcgcttc acgcccttcg gatcaagctc gtcgatttca
    37381 ccgatggctg gcaggaaggt gccgttcgcc agttcttcga ggctggaaac cgccagcgga
    37441 tgacgcagca gggatttcgg cgacatcacg accagcggac gacgcatccc gcgcagcgcc
    37501 tgacgacgca gcatgtggta aacctgtgcc ggggtagacg gtacgcaaac ctgcatgttt
    37561 tgctcagcac aaagttgcag ataacgttcc agacgcgcgg aggagtgctc cggcccctgc
    37621 ccttcgtaac cgtgcggcag caacatcacc agaccacaca tccggcccca tttctgctcg
    37681 ccagaggaga tgaactggtc gataaccacc tgcgcaccgt tggcgaagtc accgaactgc
    37741 gcttcccaga tggtcagggt gcgtggttct gcggtggcat aaccatattc aaacgccagc
    37801 acggcttctt ctgacagtac ggagtcccag acacggaacg cgccctgacc gttatggatg
    37861 tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt ggatcaccgc gtggcggtgg
    37921 aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa ccggaatgcc ttcatcaacc
    37981 agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga acagtttctc accggctgcc
    38041 atcgcctggc gatcgccata aatcttggca acgcgagact gcatttcaac tgcttctggt
    38101 accgtactga tacgtttagc cagttcctgc aggcgcttca tctcaacttt gttcgggtac
    38161 tcttcgtccc attcgtggtt gaggtacggc gaccaggtga aagagtgcat gttcatcgga
    38221 cgccactctg ccactacgca atcgccagca tccagcgcat cgcggtacag gttaaccatc
    38281 tcggtggcat cttccagcgt cgccactttt tcctgctcca gcttgtcagc gtagattttg
    38341 cgcggtgtcg gatgtttttt gattttctga tacatcagtg gctgggttgc gctcggctcg
    38401 tcggcttcgt tgtggccgtg acggcggtag cataccaggt cgatgaagac atcacgttta
    38461 aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg caacggcttc cggatcatcc
    38521 gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga tatcagtaca gtacggtgta
    38581 gaacgggcat ccagcgggtt agaggtggtg aaaccaacct ggttgttgat aacgatacgt
    38641 accgtaccgc caacttcata accacgcgct ttcgacatgt tcagagtttc ctgaaccacg
    38701 ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg gcagcacttt gttgctgttc
    38761 ggctcatcaa gtctgtccag acgggcacga acagagccga taacgaccgg gcttacaatc
    38821 tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca ggccgccatc ggtctggaag
    38881 tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc cgaggtgttc tttatgttta
    38941 ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca gcacgttcac cagcacgttc
    39001 agacgaccac ggtgcgccat cccgagaacc acttcgcggg tgccgctgtt gccagcgtgg
    39061 cggatcatct ctttaagcat cgggattaac gcgtcaccgc cttccagcga gaagcgtttt
    39121 gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt cagcggcggt cagttcgctt
    39181 aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac cagactcgat acgctgttgg
    39241 atccagcgtt tttcttcggt gctggtgatg tgcatatact cggcaccaat cgggccgcag
    39301 taggtttgct tgagggcgga aataagctcg ccgagtttca tggtttcttt gccgctggca
    39361 aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg tcagatcgtg gaaagacgga
    39421 tccagatcgg ccactttatc ttgctgccac agtcccagcg gatctagatt cgcatgctgg
    39481 tgaccacgga agcggtatgc gttaatgagc tgcaggactt taacctgctt cacattggtg
    39541 tcagggtcgg agatcgttga agagtaacgt gaagcgtctt tcgccaggcg gcggaaatat
    39601 tcacgcgttt gagagtggaa ttgatccggt ttgactcccg taccaggtaa ctgctggaac
    39661 gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta agaagtcttc atagagctgt
    39721 tctatccagc tctggtttgc gccagagagg taagaagagt ccaaccaggc tttcaaagcg
    39781 ctgttctgca tcgtgatccc ttaagcatct tttttatgct tacttcgccg tggatactac
    39841 cacgcacagt gatgtgcgtg ccggggttca cttgcgagct cttgtctacg tagtggctcg
    39901 cgaaggaacc tttaaaaact gtcttagtgt caggggcagt ttttaaagat ttcctgcatt
    39961 tctttgttac gcctgatgcg ctgcgcttat caggcctgcg ggtaatgact tgtaggccgg
    40021 ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg caacgcttgc gcgtcttatc
    40081 aggcctacag tttacgcatt acgttgcaac aacatcgact tgatatggcc gatggcgcgc
    40141 gtcgggttca gccccttcgg acatacactg acgcagttca tgatgctgtg acagcggaat
    40201 acgctgaatg catcgctcaa accgtcgagg cggctgtcag tctcggtatc acggctatcg
    40261 atcaggaaac gatatgccgc taacaagcct gccgggccga taaacttatc gggattccac
    40321 cagaaagacg gacaagaggt tgaacaacat gcgcagagaa tacattcata cagcccgtcg
    40381 agtttttcgc gctgctctgg catctgtaaa tgctcgcgag ctggcggatt ttgtccatta
    40441 ttcaacaggt aaggcttaat tttctcatat tgcgcataga attgtcccat gtctaccacc
    40501 aaatcgcgga tcaccggtaa acctggcagc gggcgaatca caatcttctt gcccggctgg
    40561 ttgagtgccg aaatcggggt aatacaggcc agaccattct tgccgttcat gttcagaccg
    40621 tcggaaccgc acacaccttc acggcaggag cggcggaacg acaggctggg atctttctct
    40681 ttcagctgga taagcgcatc cagcagcatc atgtcgcgac cttcttccgc ttccagggtg
    40741 taatcctgca tacgcggagc atcatcaaca tccgggttat agcgataaat tgaaaactcg
    40801 agtctcattt tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa tgccgggcgc
    40861 agtttcggtt ccatgttgac gcttcggcgc gtcatggatt ccgactcagg cagatacagg
    40921 gagtggcaca gccagttttc atcatcacga tccgggaagt cgaagcggct atgtgcgcca
    40981 cggctttcgg tacggaagtt ggcagaaaca gccgttgcat acgccgtttc catcaggtta
    41041 tccagttcca ggcactcaac gcgctgggtg ttgaactcgc tggaagtgtc atccagacgg
    41101 gcatttttca gacgctcgcg gatcactttc aactgctcaa gccctttcgc catcgcatca
    41161 ccttcacgga agaccgagaa gttatgctgc atacattctt gcagcgcttt acggatcgcc
    41221 accggatctt caccgttacg attattgttc caccggttca ggcgatccag agacgcttca
    41281 acatcagact cgctggcatc gtgcagtgcg ccctgctcgg cgatagactc ttgcagatgc
    41341 agacctgccg cgcgaccaaa gaccaccagg tccagcagcg agttgccgcc cagacggtta
    41401 gcgccgtgta ccgatacaca agcgatttca ccaacggcaa acagtcccgg aacaaccaca
    41461 tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa ctttggtcgg aataccgccc
    41521 atcatgtagt gacaggttgg gataaccgga atcggctctt tcaccggatc gacgtgagcg
    41581 aaggtacggg aaagctccag gatacccggc agacgggatt cgagaacttc tttacccagg
    41641 tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat cacagccgcg accttcacgg
    41701 atttcaatca tgatggaacg cgccaccaca tcacggcccg ccaggtcttt ggcgttcggc
    41761 gcataacgct ccataaagcg ttcgccatgt ttgttcagca gataaccgcc ttcaccacgg
    41821 caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg tcgggtggaa ttgccacatt
    41881 tccatatcct gcaccggtac gcctgcgcgg atagccatgc cgacaccgtc gccagtgtta
    41941 atgtgggcgt tagtggtgga ctgataaata cgccctgctc cgccagtcgc cagcacggta
    42001 gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc acagtgcggt acaacccacc
    42061 accgcgccat cctggttttt caccagatcc agcgcatacc actcggagaa aatggtggtg
    42121 tggtttttca ggttctgctg ataaagcgtg tgcaacagtg cgtgaccggt acggtcagca
    42181 gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt tcgactgacc gccaaacgga
    42241 cgttgataga tacgaccatc atcgagacgc gagaacggca ggcccatgtg ttcgagttcc
    42301 agaatcgctt ccggcccggt tttacacata tattcaatcg cgtcctggtc accgatatag
    42361 tccgaccctt ttacggtgtc gtacatatgc cattcccagt tatcttcatg ggtattaccc
    42421 agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg aacgggtcgg gaagacttta
    42481 gagagcaggg cacaggtctg gccgctctgg gaaatttgca gcgccgcgcg catacctgcg
    42541 ccaccggcac caatcacaac tgcatcaaat tctctgactg gcaatttcat cacacacccc
    42601 acaccacaac gaatccataa atcacgtaaa ccaccagtgc aacgacaatc accagttgca
    42661 gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa cacctgccac atgccgatcc
    42721 aggcatgaat caagatagaa aacagcgcca gcagggtgaa cactttggtg aacgcagagg
    42781 cgaagaaacc gatccagact tcatatgtca gttcgccact ggtggcgaaa aaaccgacca
    42841 tataaatgat gtagagcgtc aggacgatag cggtagcgcg aacgaggatg aaatcatgta
    42901 cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg aggactcctg cgagaagtga
    42961 aagcacgaca gtaataacaa aggagatttt ggcggagcgt ttacccgctt cgaatgtttc
    43021 ttccagatag ccaaaatcca tcatcatgtg gcgaatacct acgacgacgt gatacgccag
    43081 agcggtaagg atgccccaca tgataaattt gacgaagaag ctgcccataa tcgcggaagc
    43141 ttgctcgaaa ccttcagggg aagagaggct ggtacccaga agccactgca ggatgcccac
    43201 tgcaacaaag gtgatcacac cggaaacgcg atggagaatg gacgctatcg ccgtgatggg
    43261 gaaccggatg gtctgtaggt ccagattaac aggtctttgt tttttcacat ttcttatcat
    43321 gaataacgcc cacatgctgt tcttattatt ccctggggac tacgggcaca gaggttaact
    43381 ttctgttacc tggagacgtc gggatttcct tcctccggtc tgcttgcggg tcagacagcg
    43441 tcctttccat aactgcgcgt catgcaaaac actgcttcca gatgcgaaaa cgacacgtta
    43501 caacgctggg tggctcggga ttgcagggtg ttccggagac ctggcggcag tataggctgt
    43561 tcacaaaatc attacaatta acctacatat agtttgtcgg gttttatcct gaacagtgat
    43621 ccaggtcacg ataacaacat ttatttaatt tttaatcatc taatttgaca atcattcaac
    43681 aaagttgtta caaacattac caggaaaagc atataatgcg taaaagttat gaagtcggta
    43741 tttcacctaa gattaactta tgtaacagtg tggaagtatt gaccaattca ttcgggacag
    43801 ttattagtgg tagacaagtt taataattcg gattgctaag tacttgattc gccatttatt
    43861 cgtcatcaat ggattcttta cctgcaagcg cccagagctc tgtacccagg ttttcccctc
    43921 tttcacagag cggcgagcca aataacaaac gggtaaagcc aggttgatgt gcgaaggcaa
    43981 atttaagttc cggcagtctt acgcaataag gcgctaagga gaccttaaat ggctgataca
    44041 aaagcaaaac tcaacctcaa cggggacaca gctgttgaac tggatgtgct gaaaggcacg
    44101 ctgggtcaag atgttattga tatccgtact ctcggttcaa aaggtgtgtt cacctttgac
    44161 ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta cttttattga tggtgatgaa
    44221 ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc taactaccta
    44281 gaagtttgtt acatcctgct gaatggtgaa aaaccgactc aggaacagta tgacgaattt
    44341 aaaactacgg tgacccgtca taccatgatc cacgagcaga ttacccgtct gttccacgct
    44401 ttccgtcgcg actcacatcc aatggcagtc atgtgtggta ttaccggcgc gctggcggcg
    44461 ttctatcacg actcgctgga tgttaacaat cctcgtcatc gtgaaattgc cgcgttccgc
    44521 ctgctgtcga aaatgccgac catggccgcg atgtgttaca agtattccat tggtcagcca
    44581 tttgtttatc cgcgcaacga tctctcctat gccggtaact tcctgaatat gatgttctcc
    44641 acgccgtgcg aaccgtatga agttaatccg attctggaac gtgctatgga ccgtattctg
    44701 atcctgcacg ctgaccatga acagaacgcc tctacctcca ccgtgcgtac cgctggctct
    44761 tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg cttcactgtg gggacctgcg
    44821 cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag aaattagctc cgttaaacac
    44881 attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct gatgggcttc
    44941 ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga aacctgccat
    45001 gaagttctga aagagctggg caccaaagat gacctgctgg aagtggctat ggagctggaa
    45061 aacatcgcgc tgaacgaccc gtactttatc gagaagaaac tgtacccgaa cgtcgatttc
    45121 tactctggta tcatcctgaa agcgatgggt attccgtctt ccatgttcac cgtcattttc
    45181 gcaatggcac gtaccgttgg ctggatcgcc cactggagcg aaatgcacag ttacggtatg
    45241 aagattgccc gtccgcgtca gctgtataca ggatatgaaa aacgcgactt taaaagcgat
    45301 atcaagcgtt aatggttgat tgctaagttg taaatatttt aacccgccgt tcaaatggcg
    45361 ggttaatttt tatatgccta aacacaaaaa attgtaaaaa taaaatccac tatcggacat
    45421 tcatatattc taaaaaaaaa tacatcggta taaattataa acaaatagat gatttttaat
    45481 catctatctt cctctcagtt acatgaataa accaccaaat atagcgcacc cctataattc
    45541 ccaatacttt attcatcaaa catttaattc ataccatcaa ttaattactt ttacatttaa
    45601 taaaattaat aatattttta agaaaaacta aaaacattcg acaatcaaat aagatattct
    45661 tgcaccgata tacttttttc ttttctgtta aaggactcag ttatgtttaa aggacaaaaa
    45721 acattggccg cactggccgt atctctgctg ttcacagcac ctgtttatgc ggctgatgaa
    45781 ggatccggtg aaattcactt taaaggtgaa gttattgaag caccgtgtga aatacatcag
    45841 gatgatattg ataaagaggt tgaactcggt caggtgacca ccagccacat taatcagtca
    45901 catcacagcg atgccgttgc tgtcgacctg ctcttagtca actgtgatct ggaaaactcc
    45961 agcaacggtt ccggtggcaa gatttctaaa gttgcagtca cctttgatag ctcagcgaaa
    46021 accaccggcg cagatccgat tctcaacaac accagcacgg gtgaagccac cggcgtcggc
    46081 gtacgtttaa tgaataaaga tcaaagcaat atcgttttag gcaccgctac tccagatatc
    46141 gacctggctc cgacctccag cgaacaaacg ctgaatttct ttgcctggat ggaacaaatt
    46201 gatcaggcta cacctgtaac gccaggcgca gttacggcga atgcaaccta cgtgctggat
    46261 tataaataag tattactttg gcggcgctat cgccgccttt cataaaatat ttatcaggta
    46321 tggacaccgt gaatatttat cgactctctt ttgtatcctg cctggtcgtg gcgatgcctt
    46381 gcgcattggc ggtcgaattc aatctgaatg ttctcgataa atcaatgcgc gaccgcattg
    46441 atatttcatt attaaaggaa aaaggagtca ttgctcccgg tgagtatttt gttagcgttg
    46501 cggtaaataa taaccaaatc agtaacgagc aaaagattaa ctggcacaaa aatgacgata
    46561 aaaccattcc gtgcatcaat gatttactgg tcgataaatt tggcttaaaa cctgaagtcc
    46621 gtcagtcgtt accattgata aatcagtgcg tcgattttag ctcccgacct gaaatgctct
    46681 tcaatttcga tcaagccaat cagcaactga atattagtat tccgcaagcc tggctggcgt
    46741 ggcactcaga aaactggacc ccaccctcca catggaaaga aggtgtcgcc ggtgtcctga
    46801 tggattacaa cttgtttgcc agcagctacc gcccacagga tggcagcagc agcactaatc
    46861 ttaacgccta tggaacagcc ggaatcaaca cgggggcatg gcgcttacgc agtgattatc
    46921 agttgaatca tactgatagc gatgataacc atgaacagtc aggcgaaata tcacgtacct
    46981 atctttttcg cccgttaccg caattaggtt caaaattaac cctcggcgaa acggatttta
    47041 gtcccaatat tttcgacggt ttttcttata ccggcgcggc actggcaagt gacgatcgaa
    47101 tgttgccatg ggaactacgc ggctacgccc cacaaattag cggtattgca cagaccaatg
    47161 ccacggtgac gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc ccaccaggcc
    47221 catttatcat tgacgacctt aatcagtctg ttcagggcac actggatgtc aaagtgacgg
    47281 aagaagatgg tcgggtgaac aatttccagg tttcagcagc atcgacgccc ttcctgactc
    47341 gtcagggaca ggttcgctat aaactggccg cgggtcagcc acggccctcc atgtcacatc
    47401 aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg ggggatgctg tcaaacacct
    47461 cgctgtacag cggcctgctg ctttctggtg atgactacca ttctgccgca atgggtattg
    47521 ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt cacgtgggcc agtagccatt
    47581 ttgatactca gcaggacgag cggggcttaa gctaccgttt taattacagc aaacaagtgg
    47641 atgccactaa cagcacgatt tccctcgccg cttatcgttt ctccgatcgt cattttcaca
    47701 gctacgccaa ctatctggat cacaaataca acgacagcga tgcgcaggac gaaaaacaga
    47761 cgatcagctt atctgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc
    47821 tgctacatca aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt
    47881 ttaatgttga tattggtgac tggagagata tctctatttc tacgtcattc aatacaaccc
    47941 attacgaaga taaagatcgc gacaaccaga tttacctgtc gatttcgctc cccttcggta
    48001 acggtggtcg ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt
    48061 cgtggaacga tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg
    48121 accgtcctga caatggagcc caggtgagcg gtaactatca gcacctgagt tcagcgggtg
    48181 agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga
    48241 gcggttcttt caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg
    48301 aaccacgcct gatggtcagc accgatggcg tggcagatat tccggttcag ggcaatctcg
    48361 actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca
    48421 ccgtggcggt gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca
    48481 aagaaacgtg gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag
    48541 acgttaacgt catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc
    48601 gccaggatga cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa
    48661 gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc
    48721 tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat
    48781 taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct
    48841 gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa
    48901 agctaccagt ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg
    48961 gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca
    49021 acgtctggaa ccgaaagcaa caacacaggt acggatagta aagcaagcct cgaccacaaa
    49081 attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc
    49141 ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt
    49201 ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt
    49261 cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc
    49321 ctacctggga cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc
    49381 ttttagtacg gtcaacacaa acaccggaaa ttatagcggc agccagtttt atctcggtta
    49441 tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccattt
    49501 acaagccgtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg
    49561 ttatttctgt cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc
    49621 tcggtagaaa aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc
    49681 gataagatct gggaatctga cgatattaaa ataagatctg ggaatctgac gatattaaaa
    49741 ttcccgtcta ttgtgacaac aataccaacg gcaactttga aagcgaacat gtctacgcat
    49801 gggtaaaccc ctacccgggg gtgcaggatc gttattatca actcggagta acttacaatg
    49861 gcgttgatta tgacgccaac cagggaaaaa gccgcatcga cactaaccag tgtatcgaca
    49921 gtaagaacat tgacatttat acccctgatg ggatggcaga acaaaatttg ctcgggtgat
    49981 cccgctaata ttcacatgtc gcgaaccttt ctcgctcgca tgcggttata cgtcaaaata
    50041 cgagaaatgc cgccgcatga ttatcaaagt acgcttagcg actatatcgt cgtgcaattt
    50101 gacggtgccg gtagcgttaa cgaagaccct actgcccaaa acctgaaata tcatattact
    50161 ggtctggaaa acattcgcgt gctggattgc agcgtcaatt tttccatttc accggaaaca
    50221 cagatgattg attttggtaa atttaatttg ctggatattc gtcgccacac aatgtcgaaa
    50281 acattcagta ttaagacgac aaaatcacaa aatgaccaat gcaccgacgg atttaaagtc
    50341 agttcctcct tttataccga agaaacgttg gttgaagaag ataaagcatt gttgattggt
    50401 aatggtctga agctacgttt gctggatgaa aacgcctcgc cttatacctt caataaatat
    50461 gctgaatacg ccgatttcac cagcgacatg ttggtctacg aaaaaaccta tacagctgaa
    50521 ctttcgtcca ttgcaggtac ccccatcgag gctgggccct tcgatacagt ggtgcttttt
    50581 aaaattaact ataactgatt tgccatgatg ccctttgcag ccggaaaggg catctctttc
    50641 agaaaattgc tgattttccc cttttccttg attgctgtta ttttttccta gcccggcttt
    50701 ctttatgctg tatcgataca ccaaagagaa aataatgagg gagcgaagga tgccagtttt
    50761 gcagtgggga atgttatgtg tgctatcact tctcctttct attggtttcc tcgcggtaca
    50821 tctcccggcg gcgctattac ttgggccgat gatcgccggg atcatcttca gtatgcgcgg
    50881 aattactctg caacttcccc gctccgcttt tctcgccgcg caggccattc ttggctgcat
    50941 gattgcgcaa aacctcactg gttcgattct caccacgctg gccgccaact ggccgatcgt
    51001 gcttgctatt ttactggtga cgctgctttc cagcgccatc gtgggttggt tattggtgcg
    51061 ctatagctca ctgcctggaa ataccggtgc ctggggttct tctccaggcg gcgcagcggc
    51121 aatggtcgct atggcgcaag attacggcgc agatattcgc ctggtggcgt ttatgcaata
    51181 tctgcgagtg ctgttcgtcg cgggggccgc tgttctggtc acccgcatga tgctgggtga
    51241 taacgctgaa gcggttaacc agcaaatcgt ctggttcccg ccagtgagca ttaatctcct
    51301 gcttacgatt ttgctcgcag tcgttgccgg tacggcggga tgcatgctgc gtcttccttc
    51361 cggcacgatg ctcatcccga tgctggcggg cgcagtgctc cagtctggtc agctcatcac
    51421 catcgaacta ccggaatggc tgctggcgat ggcgtatatg gcaattggct ggcggattgg
    51481 tcttggtttc gataaacaaa tcttactgcg ggcactacgc ccgctaccgc aaatcctgct
    51541 gtcgattttt gctctgctgg ctatttgtgc aggtatggcg tggggactga cccggtttat
    51601 gcatattgat tttatgactg cctacctcgc caccagtccc ggcgggcttg ataccgtggc
    51661 ggtcatcgcc gcagggagca atgccgatat ggcgcttatt atggcgatgc aaaccctgcg
    51721 cctgttcagt attttgctga cggggccagc cattgcgcgg tttatttcaa cctatgcgcc
    51781 gaagcggtcg gcctagtgct ggcagcctgg acaccagtaa aacgggcgcg aggacaacgt
    51841 ggttttctca atgatgccgc cacagcgttc gcacagttcg ccatcacgat gaaaaacctt
    51901 aaagcgaaac agcgccccat gatgcttatt ctcatccacc tgcccccgcg tagcgtaaga
    51961 aaaccgagga atctccagta acgcgtgtgc gagtgcatcc agttgcgccg cattgagatc
    52021 tttcgcttta tgatttccag tcaacccaac ctgccagagg atctccaccc gcaaataatt
    52081 gccaagccca gccagaaacg cctgatcgag cagtaatcct gcaaactgac ggttacgaaa
    52141 gcgcggcgac aataaccgtt cctttaccac ctccggcgtc agattcggat ccagcacatc
    52201 gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt tccggcctca acatctcaat
    52261 atccgaggcg ctataaagca gaatggtttt gtcagccgtt tgcagtttta cccgcaatac
    52321 tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc cagacgccgt aaagctgatt
    52381 atggctgtag agcgttaaac cgttgggaaa atgagttaac aacgccttac cacgcgtttc
    52441 cacatgggta acgtgttgac cgataagttg tgattgataa gtttttaact gcgggaaggc
    52501 aaaccagaca tcagttagtg gtttgccttt gatcgccgcc tccaggttat ccgctgcacg
    52561 gcggatctcc gggccttcag gcatggtgat atcctttttt cattctttat taagcggggt
    52621 tatgctgcga caacaatccc ctttttggca aatgtagctc gtaatcggcg ggcgaacgcc
    52681 agagcgtgct cgccgtcgcc atgcaggcag accgtttgcg ccgcgaccgt tgcccattcg
    52741 ccggtgatac ttttgactct gccgtgttgc accatctcca gcgtttgcgc caacgcctgt
    52801 tcttcgtttt caatcaacgc gcctggctga cttcgcggca ccagcgagcc gtcagcctga
    52861 taaccgcgat cggcaaacac ttcctcgcgc gttgtcaggc catattgctt gcctgcacga
    52921 atcagctcgc ttcctgccag cccaacgaga accaatgccg gatcgcaagc gtataccgct
    52981 ctggcgatgg cgtctgccag ttgtgcttct ttcgccgcct ggttgtacaa catgccgtgc
    53041 ggtttgacat gacgcattac gccgccttgc gcacgggcaa tcgttgccag cgcgccaatt
    53101 tgatacagcg tctgggcgta aacggtttcc ggcggcagct gcatggcgct gcgaccaaaa
    53161 ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga caccattttt tattgcttca
    53221 cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa atccacaagc aatattggca
    53281 gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc cttcgcccag atcggcgttc
    53341 aggtcaattt tcattgtgca gccgccacgc taattgttca aaataacgtt gctgatcttg
    53401 ccgcgctttc agtgcctctt ccagtgaaca ctggacaaaa tgaatcggct gaccgagcgg
    53461 aatttgcgcc agatggtaca tatcagcctc aatgatacag gcaatacgtg gataaccacc
    53521 ggtggtctgt gcgtcgttca tcaacacaat cggctgcccg ttatgcggca cctgcaccac
    53581 gctcggtaac aaaccgtgag ataacagttc gcgatcggtg atgcgtttta aaatttgccc
    53641 ctgtaaacga tagcccattc ggttactttg cgagctaagc tgccagggcg aacgccagaa
    53701 tgcatcctgc gaggcgcgat cgaactcatg atattccggc cccggcaagg cgcgaatgcg
    53761 gttgccccac agcagctgtt taacgccctg cgcttccata aaatcacgct tcgatttgcc
    53821 aatcgggagt cagtcaccat ccttcagtaa acggccttcc agcccgccaa tccccacttt
    53881 gagatcggtg ctgcatgacc ccattaccgg cggaacatca ataccacccg cgaccgccag
    53941 ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg ctttcatcgg
    54001 caatcgccag ccggtccaga cggcgttatc atccaaccgc gcttcgcaac ctgcacccgt
    54061 cagggcaaac cacccatcag tttcgaactc aactgttaac tgaccgagcg tgatctccag
    54121 cgcgggggca ttggcgtcat tacccaccag caggttagca atgcgcagcg caggcatatc
    54181 cagtgcgcca cagtggctga tacccgactg gcaaaaaccg tgacgaccgc catcctgcac
    54241 agtggtgtac atgcccgcac gaataatctt cagcatattc cctccttctg cggtacaaat
    54301 cgcacgctgt ctcccggacg taataagatg ggttcgtcac gcgccggatc aaacaggctg
    54361 agtgaggtat gaccaatcaa ctgccagcca cccggcgttg ccagcggata aacaccagtc
    54421 tgcggcccgc cgatcccgac agaacctgcc ggaacgagca agcgcggttc agcgcgccgt
    54481 ggcgtgtgta gttgttccgg caaactcccg agatacggga agcccggttg aaaacctaaa
    54541 aaccagacca cgtattccac ggaggagtgc aattcaacaa cctgtttttc gctcaacccg
    54601 caatacgccg cgaccaccgc caaatccggt ccgcctgcac caccgtaaac caccggaatt
    54661 tcaataaagc gagactcagg ttccagggcc tcgctctcct cccaccagcg ttgcaaacgc
    54721 tctatggcat ccagcgccag cgactcagga ttacgcaaaa tcaccgtgat attgttcatg
    54781 ccgggaatgg cttcaaccac attcggcata tccaccagac gctgcgccag tcgccagatc
    54841 cgtttctggc tagccagcgt caccggcggt tccagttcca gcactaccgc cgtttcacct
    54901 atcagataac aacgcgctcg ttgcactttc gatccctctg attattcgtt atgcaggatt
    54961 aggaatatca ataaaggtca catcaagatc ggtattttca ttcagccact cgctcaatgc
    55021 gcgaatacca ccgcgttcag tggcatggtg acctgcagca taaaaatgca atccctgctc
    55081 gcgggctgaa tgaatggtct gttcagaaac ttcgccagta ataaaagcat ccacgccaaa
    55141 acgcgcggcg ctatcgataa aactttgccc gccgcccgtg caccaggcga cgcgctggac
    55201 cacctcaggt ccggtatcgc cacaccataa cggcttacgt cccagacgcg cttcaatcca
    55261 ggaggctagt tccattcccg gcacaggcat ggtcagctct ccccacggca ccaacggctc
    55321 aatttcgccc atgaccgtga tccccagtaa cgccgccagc tgtgcgttat tgcccagctc
    55381 aggatgtgcg tcaagcggca aatgccagcc atacaggttg atatcattcg ccagcaacgt
    55441 ttttaaacgg ttacgcttca ttccgcgaat gaccggagac tctcctttcc agaagtagcc
    55501 atgatgcaca atgaccgcat ctgcgcccag acgcactgcc tcatcgagca aagcctggct
    55561 ggcggtgaca ccggtaacaa ttttttgcac cgtctctttg ccttccacct gcaaaccatt
    55621 cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt gttccagttc
    55681 ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta caccgttaac atagcgttac
    55741 atcgcctgtt cgtcgatagc caatcttttg acatattaag aaacaaaccc gacgccaaaa
    55801 gacgcaaatt gtttatatca tcacgcttca gctaatgtat atttatgcaa tcagacagca
    55861 tattgcataa ataatcagat ctgtatcaaa gttttaactt taaggaaaca caagcacgcc
    55921 gcgcaactat tacgtgacgt tgccgtcaat tgtggaatta ttatgaataa acacgcatca
    55981 caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct gggaatattt tagcttttac
    56041 ggcatgcgtg ccctgctgat tctctatctc accaatcaac taaaatacaa cgatactcac
    56101 gcctacgagt tatttagcgc ctactgttcg ctggtgtatg tcacgccaat cctcggtggc
    56161 tttttggcgg ataaagttct cggcaatcgc atggcggtga tgctgggggc gttgttgatg
    56221 gcgatcggtc atgtggtgct gggtgccagt gagatccatc cgtcattcct ctatctgtcc
    56281 ctggcgatta tcgtctgcgg ctatggcctg tttaaatcta acgtaagctg tctgctcggc
    56341 gagctgtatg agccaaccga tccgcgtcgt gatggcggtt tctcgctgat gtatgcggcg
    56401 ggtaacgtgg gatctattat cgcacctatc gcctgtggtt acgcccagga agagtacagc
    56461 tgggcgatgg gctttggcct ggcggcggtt ggcatgatcg cgggtctggt cattttctta
    56521 tgtggcaatc gtcatttcac tcatacccgc ggcgttaaca aaaaagtact gcgtgcgaca
    56581 aactttctcc tgccgaactg gggatggctg ctggttctgc tggtggcaac gcctgcactg
    56641 attaccgtgc tgttctggaa agagtggtcg gtatacgcct taattgtcgc gactattatt
    56701 ggtctgggtg tactggcaaa aatttatcgc aaagcagaaa accagaaaca gcggaaggag
    56761 ctggggctga ttgtgacgct caccttcttc agtatgttgt tctgggcctt cgcacaacag
    56821 ggcggtagct cgattagcct ttatatcgac cgcttcgtta accgcgatat gtttggttat
    56881 accgttccga ccgcgatgtt ccagtcgatt aatgccttcg cagttatgct gtgcggtgtg
    56941 ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc gtaccgtgcg catctgggga
    57001 aaatttgctc ttggccttgg cctgatgagc gccggattct gcattctgac cttaagcgcc
    57061 cgctggtccg caatgtatgg tcactcttct ctgccactga tggtgttagg cctcgcggtg
    57121 atgggctttg cggaactgtt tatcgacccg gttgccatgt cgcaaattac gcgcattgaa
    57181 attccgggcg tgaccggcgt attaaccggc atctacatgc tgctttctgg cgcgatcgcg
    57241 aactatctgg cgggcgtgat tgccgatcag acatcgcagg cttcgtttga tgcttccggg
    57301 gcgatcaact actccatcaa tgcatatatt gaagtatttg atcaaattac ctggggcgca
    57361 ctggcgtgtg taggactggt actgatgatt tggctgtatc aggcgctgaa attcagaaac
    57421 cgcgcgctgg cgctggagtc ataataggcc agcaccgatg cctgatgcga cgcttgacgc
    57481 gtcttatcag gcctacgtat ttcctgcaat ttattgaatt tgcacaaatt tgtaggctgg
    57541 attaggcgtt cacgccgcat ccggcatctg ggctcgattg cctgacgcgt ctgttatttc
    57601 cccttccgcg ccgcctcata cgctgccaac gtttgtactc tcgcctcttt gtgctcgact
    57661 atcggttgcg gataatccag cttcacacct gctttctgcg cccacttcca cggctcatgc
    57721 accgctttcc ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca
    57781 cggtcaaatt tctcgccctg agttatcgga ttgaaaatac gaaaatacgg cgcagcatca
    57841 gtacccgtag aagccgccca ctgccagcca ccgttattag ctgccaaatc accatcaatc
    57901 agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga
    57961 aaactggctg taatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc
    58021 atagcggcat caataatcgg gtatcccgtt ttgcctttct gccaggcctg taaatgtgcg
    58081 ggattgcttt gccactgcac acgatccgtc caggcaataa atggacgatg tttacacaac
    58141 gaggggtaat acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag
    58201 acactaccgg ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac
    58261 tggcgaggcg ataacccgcc cgttgccaag ctggcggaca aacggctggt gccttccact
    58321 gccggaaaat ctcgttgttg ctcatactct ccagcaccgt tctcgcaaaa ctggcgtaat
    58381 tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga
    58441 taattcagcg taatggatgg cgcgggcttt atcgatccgc tactacgaac ttttggtgca
    58501 gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta
    58561 aagactttgt acatctcgtg attgccggtc atcaccgccc ccggcggcag gatcacgctg
    58621 tcatcaaatc cttcacacac cacgttacgc agcgctcttt caacgtccac atcccgcgct
    58681 cgctcattca cctcatactg atagttataa aaaagatgag taacgctgtt ttccgcgcac
    58741 acctgtttaa ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga
    58801 ataccttttt ccgccagcgc gatttgtagc ccattcagtt gagcattgat aagttcagcc
    58861 tgacgcggcg acatgttatg cgtctcccac tggcgtggtg tagcgatata cagcgccagc
    58921 acgcgtgcag acgaattgcg gcaggcggca gccagtgcga gattatcgtg cagacgtaaa
    58981 tcctggcgaa accaggccag atgggtagtc ataaaactcc tgaatggcgc aagttaatca
    59041 cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca
    59101 tatagtgttt gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt
    59161 cgataagcgc agccagctgc tggcgttctg tggaatcaat atgcgggcga aaataaccct
    59221 gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa
    59281 ttacgcgttg gcggtactgg ttgtaaaaat catccagatt ttgccactga tgaatatcag
    59341 ccacaaacgg gccaagtttg cggtactcgg gctgagaatg ggccagaaag acgagtttat
    59401 agcggctgtg ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcgatgta
    59461 actcatgaag ggcgaagata cggattaggg tgctgtcatc aaatcgttgt agattcatta
    59521 ctgctcctga acggtataca ggagtaagtg taggcgttgg taaatgatgt ttgtgaagtt
    59581 aatccgctag cagggcaaga ttacgaaagc gcgctccccg caagcaacta atcaaggtag
    59641 ttaatcatgg ctgaacacga tattacgcca gaagtcacca tttccaaaac acagcgccgc
    59701 tataaagtcg gctatgtcag agcccgccac caggaccgca aaaccggtat agcccggtat
    59761 tattcacaac atcccagcct gcaccttaag ggcgactggc tgaaagaggc cggatttgat
    59821 accggacggg gtgtcacggt gaagatttcg gaggggggcc tgaccatcat cgctgacagt
    59881 gacgaggtgc aggaactgcg ggatgggatg tttagtgtgc tgaacgaaag ttaaatatta
    59941 aaaaagctgg gaagatttaa aaccttccta gctttttatt ctgtcttatt ataatggata
    60001 ctcatcatct tccgatgaat ttataaattg ctccaagttt acatgaagag tacttaacct
    60061 agtttttaac tcattaagtg aagcgataat ttcatatttt ccaacataat catatgatgg
    60121 atcgtattca ttatctatgt cagaagctaa aaatgatgat attcgtagtt taaatttagc
    60181 ttcatcttta ttacattcat tggcataagg tgaatgccct atcatagcat attcatctaa
    60241 tctaaaagga taagatggtt ttaattcatc gatcaatgaa atttcaatcc atgttctata
    60301 tccaggcatt aatggactaa tttctttgag caaaaccttt ccacttaata atgtattgag
    60361 attgttcatt attatgtctc ccacgggaaa aatatatgtc taacacttcc accatgttga
    60421 tgaacttgta aatgtggtaa atcaccatca ggatttccta gtccatgacc tgacactcta
    60481 tatgaaggtt ttgttacaga aggatctttt atatcatccc aatgatatga acctgctttt
    60541 gttttatttc cagtaagata ttccattaag ttcttagtgc tggggcctga ctcacctgtc
    60601 atatttttat aattatgacc taaatatcta tccataaaca attgttcagc gtcacttcta
    60661 gacctaacag taactttggt ttcactacta tctaataaac gattcgcttt ttggttacta
    60721 acagcacatg gagttaatcc aagtggatca gcatatttta gaggattagg tgcataactg
    60781 tagagattta aaccgcctct caaccctatc gggtcagtta ccatgaaccg cccgacatcc
    60841 ggatcatagt acctgtgcag attgtagtgc agccccgttt ccctgtcgag gtactgtccc
    60901 tggtagcgca ggttctgctc caccccgccg tggtccttct cctgtatcgt gttaccccag
    60961 acctgatacc ccgtctccca cacgattttc ccgtcgctgt ccgtcatctc ttccggcgcg
    61021 ccgttcacat ccgtgtgaaa atacagtatc cggttcgggc cctctttgcc cgccttatcc
    61081 acccgcgcca gcggttcata actcccctgg tccctgtaga tgtacaggct gcctgttccc
    61141 tccggcctgc tctcctgcgc cagccgcatt ccgtcccata aaaattttgt ccggttgtac
    61201 ggcttaccgt cagggctgat gtgctgtttt tccgtccgcc tccccagtgc atcgtacaca
    61261 taccggtatg tcccccgcgc tgtcgtcact tccaccatcc ggtgttccgc atcataacgg
    61321 taactctgcg ttccgctccg gccctccttt tccaccgcac ggccaaattc gtcataacgg
    61381 tagtgcacac cacggtaaga catcagccgg ttgaacggca ccacgctgtt ttctgccgtg
    61441 gccctttccc ccgcgcgcct ccccagcagg tttcccgccg cgtcgtactg ccagctcgcc
    61501 agatacccct catcctccga acgacacgcc gtgatgcgcc ccgtggggtc gtagaagtaa
    61561 tccgtttccc cccgccggct gtgccgcgtc ttcagcagtt catcctcgcc actgtagctg
    61621 tactgccggt ccgtgaacgt ctccggtacc accccgcgca tcccgctgta tgtctccctg
    61681 cgcttcagcc gcccgccggg gtcgtaaagc tgccgcgtgc tcagtcgccc ctggcttctg
    61741 agcacttcac ggtgcaggtg gtcgcgcgtg aactcgctga tgacctcccg gtccagattt
    61801 atctgctgga gatggccgct gccgtagtac agatggcgct gcgttctgcc gtccggaaag
    61861 gtggtggcag tccggttgcc cagtttatcg tactggtggg tcaggatgcc ctgcgccgtc
    61921 tcctcgctca gcatccgccc cagtctgtcg tagctgaagc ggataacttc cggcgcgtca
    61981 gtttcgtgcc gctggcgacg gatttccagt agctggtctg ccgcatcatg gacgtattcc
    62041 gtacggccat cggctgtctc ttttgccgtg agccggcccg ccgcatcacg cgccagccgg
    62101 tggcggattt caccgccccg gtccccggtg aacaccactt ccgtgaccgc gttcagcgcg
    62161 ttgtagcggt acgactggcg gcagccgtcc gggtttatct gctccgccag acggtccagc
    62221 acatcatggc ggaaccggta gcgttctccg ttctcgttcg tgagcgtgac cagacgcccg
    62281 taagcgtcgt acccgaatgc cgtcctgcgc cccatcgcgt ccgtccggct gcgcacccgg
    62341 ccctgcccgt cccgctgcca gcgcgttatc ctccccatcg ggctggtgta ctttaccagc
    62401 ctgcccgccg cgtctgactg gtaattcacc gtgcggccgt ccgccagtat cacctccgtc
    62461 agatgaccgc tgtcgtcata cccgtagcgc gtgctgtggc tctgcgcatc cgtcattctg
    62521 ataagcctcg tgcgttcgtc gtagaaccac gccgtctgcg agccggaaca gtccgtgtgc
    62581 cgcatgagct gcccgtcttc attccactgg aggtatttat tccccccgcg cgcatccgtt
    62641 atctgcacca cctagccgtg gtggtcatac tgataccggg tgttctgctg cagcgggtcg
    62701 gtcacggcaa gaaggttgcc gcgttcatcg tacacacagc gccaggtgtt tcccgccgcg
    62761 tccacctccg tttccggctg gtgccacacc gggtgccact gcgcctgctc cacccgcccc
    62821 agcgggtcgt gcgtctccgt gatatgcccc tgcaggtcat acacatagcg ccatttgccg
    62881 ccctgagggt cggtcatccc cagcaacagt cgttcttcgt cgctccagcg gaatgtcgtc
    62941 acctgtccgg cttcgtcctg gtacgccgtc acctgattct gcgcatccca ctggtgataa
    63001 cgtgtgcgcc ccagtccgtc cgtcacgcac agcctgcgct gcgccaggtc ccacgccagc
    63061 cggtactgtt caccgctgtc cgtgtggtgc cccgtcaccc gccatgccgc gtcatccgga
    63121 ccttcatacc agccccactg gtaataatac ccttcgccgc ccggctggcg gtgcagcgtc
    63181 atgcagtggt tttcgtcgta agcaaactca cgcagcaggt gccccgtgct gtcgtacacg
    63241 ccgttcaggt ggccggcctc gtcatagctg taacgacgca gcagtaaagg tgcagtctca
    63301 gggaaatgct gataaatctg tgtcacccgc cggggatgcg ccgccagttc gtaatacaga
    63361 cggatgcacg gacgctgctg ttcgccgctg atttccacaa tgcggccccg gtcgtcgtag
    63421 tacagattca gacagttgct gttacggtcg cccagcattt tcagccgctg gcgctgcggg
    63481 tggtgcgggt cagcctcaaa cagaaaacac tgcccgtcat cgctgctgat aagccagtgc
    63541 ccctgctcac cacgccttac cgccagccct tcaccggggc tgtaaaaccc gccccccggc
    63601 agtacggctt ccatatccaa gcgtcgcccc atgcccgaca caaaggtcat gcagttttcg
    63661 tctgcgctac ccggtgtgca cacgaggctg actttataca ttacactcca gccctgacca
    63721 aacattcctt ccgtacgttc gtcacgactg ctgtacacgc gctgccagtc cagcggaaag
    63781 tggcccggca gactgaaatc gaagtcatcg tcatcggcca ggtatttggc cccggtggcg
    63841 gcattgaccg gatgcggaag ggcactggtg gccttctgcg tcagcatgct catcccgaca
    63901 ctggcagcaa aacaggcgat tttagaaagc attttgcccg ggcgaagaaa catcagcgcc
    63961 acggttgcca ccagggttat ctgcgacctg ccgcttctga tatccctgac caccagtaac
    64021 ggaccgccaa tgcgcacatc cggtgacaca aacgccccgt tccccggctc attcaccacc
    64081 ctggcctcgc aggtacagcg tgccccgctg cgtgtcgccg gctggctgtt gatacgcaca
    64141 tcccgcgcgc cttccgcgat atattccgct gcgggcggct cgtcatcgcc tttgaacaga
    64201 ttactgatgc tgccaataac cgcattcact ccctgaaagg cggtttccag cataccgccc
    64261 ggattcagca ggttaagggc cgttcccagg gggtcatcca gaaaggcggc ttttttttgt
    64321 gcgagaaact gcgtcagtga ggaaggatgt ttatggcagt cgattttatc gtcaggggtc
    64381 ggggtggatc ccggggccgc actggccaca gtgggctgcc atagttccga cacaaaatgt
    64441 gtcacgccat ccaccatttc agaacgccct tcttccgtac tgagctggtc gtgcagagcc
    64501 cgggacatca gcacaggccc tgtcaccggg ttaaccgcca gggctatggc ccccgccatt
    64561 tccagatatt ccccggccac atcccggaac gttctggcat cctcctgacg ctgcgcttct
    64621 tcctgttctg ccttcgcctg cgcttcctgc gcctctatgt cctgccgcga tgccgcagtc
    64681 gccgctgccc gcgcggcagg tttgctgttg atccatgtgt caccagagcc ggtgaggatg
    64741 taaccttcaa tctgaggcgg gaaaagcata tttgcgaggg ctgaacaggc ttcgctgacc
    64801 ttttctgtca cgccggtgta caccatcgcc acacccgcca caaaaccggt cagcacgacg
    64861 gccgtgcacc ccatcgtgat ccccactgac gtcgccagta acgaggttgt gccgacaatg
    64921 agggcatcaa taacgacatt tgcggccacc tccagcaccg cccccagcac atctgccata
    64981 aacgacgtat gcagcagggc atcaccctgt cttgccgccc agaatgcctc ttccatgtcc
    65041 cgttccctgt atgattaatt cgtcctgact tcctgccgca tcctgaagcg cagattcttt
    65101 ttaatttcag cccagcgcgt catatctgcc ggggtcagcg gtgtggttct ggcgtaggtg
    65161 aaggccatca tgacccgctc acccggcaat acaaatgcca cctgacactg gtacacctgc
    65221 tcgttacccc ggagaaactg gctctgtatt tccaaaccgc ctcgttgaga taatattccg
    65281 tcataatgta aatagtccgt tttaataaag gaattattta ttgacaggtt ctgttttttt
    65341 aatcatgaat gtaataccca tcaccgcttt acagcacact ttatctttcc agccataatc
    65401 actaataata ttaagcaatt agcaatattt acaacgacca gagcggatac atagttatgt
    65461 atttaacaat cccaatatat tcactatgca aataaaacca ctagtaacag acacgctacc
    65521 gcctccatat aaaaatggac accttataaa attgtatctt gcatagaaaa agctgaaata
    65581 tcggatgcgt tttaattatc aactataaat ctataagctt tttattaaca aca
//
LOCUS       DAEEDS010000011        65614 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEEDS010000011 DAEEDS010000000
VERSION     DAEEDS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.011"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KLH32_000746"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KLH32_000746"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBE0137697.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KLH32_000747"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KLH32_000747"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBE0137698.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KLH32_000748"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KLH32_000748"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBE0137699.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KLH32_000749"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KLH32_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBE0137700.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KLH32_000750"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KLH32_000750"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBE0137701.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KLH32_000751"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KLH32_000751"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE0137702.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="KLH32_000752"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="KLH32_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBE0137703.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KLH32_000753"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KLH32_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE0137704.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KLH32_000754"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KLH32_000754"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBE0137705.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KLH32_000755"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KLH32_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBE0137706.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KLH32_000756"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KLH32_000756"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBE0137707.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="KLH32_000757"
     CDS             complement(15665..16702)
                     /locus_tag="KLH32_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0137708.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="KLH32_000758"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="KLH32_000758"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0137709.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KLH32_000759"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KLH32_000759"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBE0137710.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="KLH32_000760"
     CDS             19444..19884
                     /locus_tag="KLH32_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBE0137711.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDNSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KLH32_000761"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KLH32_000761"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE0137712.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="KLH32_000762"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="KLH32_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KLH32_000763"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KLH32_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBE0137713.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KLH32_000764"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KLH32_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBE0137714.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KLH32_000765"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KLH32_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBE0137715.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KLH32_000766"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KLH32_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBE0137716.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KLH32_000767"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KLH32_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBE0137717.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KLH32_000768"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KLH32_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBE0137718.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KLH32_000769"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KLH32_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBE0137719.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KLH32_000770"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KLH32_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBE0137720.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="KLH32_000771"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="KLH32_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBE0137721.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KLH32_000772"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KLH32_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBE0137722.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KLH32_000773"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KLH32_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBE0137723.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KLH32_000774"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KLH32_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBE0137724.1"
                     /translation="MNKRDAINHIRQFGGRLERFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KLH32_000775"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KLH32_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBE0137725.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KLH32_000776"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KLH32_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBE0137726.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="KLH32_000777"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="KLH32_000777"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBE0137727.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="KLH32_000778"
     CDS             37412..37681
                     /locus_tag="KLH32_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBE0137728.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="KLH32_000779"
     CDS             complement(37684..38043)
                     /locus_tag="KLH32_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBE0137729.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="KLH32_000780"
     CDS             38184..38810
                     /locus_tag="KLH32_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBE0137730.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="KLH32_000781"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="KLH32_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBE0137731.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KLH32_000782"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KLH32_000782"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBE0137732.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="KLH32_000783"
     CDS             41650..42315
                     /locus_tag="KLH32_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBE0137733.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="KLH32_000784"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="KLH32_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBE0137734.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="KLH32_000785"
     CDS             complement(42949..44166)
                     /locus_tag="KLH32_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0137735.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="KLH32_000786"
     CDS             44300..45349
                     /locus_tag="KLH32_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE0137736.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="KLH32_000787"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="KLH32_000787"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBE0137737.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="KLH32_000788"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="KLH32_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBE0137738.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="KLH32_000789"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="KLH32_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBE0137739.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="KLH32_000790"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="KLH32_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBE0137740.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="KLH32_000791"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="KLH32_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBE0137741.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="KLH32_000792"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="KLH32_000792"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBE0137742.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="KLH32_000793"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="KLH32_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBE0137743.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="KLH32_000794"
     CDS             complement(51544..51903)
                     /locus_tag="KLH32_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBE0137744.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="KLH32_000795"
     CDS             complement(51900..52175)
                     /locus_tag="KLH32_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBE0137745.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="KLH32_000796"
     CDS             complement(52234..53358)
                     /locus_tag="KLH32_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0137746.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KLH32_000797"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KLH32_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBE0137747.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="KLH32_000798"
     CDS             complement(56090..57157)
                     /locus_tag="KLH32_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0137748.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="KLH32_000799"
     CDS             complement(57523..59145)
                     /locus_tag="KLH32_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE0137749.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="KLH32_000800"
     CDS             complement(59407..60996)
                     /locus_tag="KLH32_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE0137750.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENCFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="KLH32_000801"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="KLH32_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="KLH32_000802"
     CDS             complement(62499..62975)
                     /locus_tag="KLH32_000802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137751.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="KLH32_000803"
     CDS             complement(62968..63732)
                     /locus_tag="KLH32_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBE0137752.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="KLH32_000804"
     CDS             63978..64739
                     /locus_tag="KLH32_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0137753.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="KLH32_000805"
     CDS             complement(64846..65346)
                     /locus_tag="KLH32_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137754.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15937 a        17624 c        16160 g        15893 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggataatt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgtcgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgctccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaac aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcct
//
LOCUS       DAEEDS010000012        60383 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEEDS010000012 DAEEDS010000000
VERSION     DAEEDS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60383
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.012"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..322
                     /locus_tag="KLH32_000806"
     CDS             <1..322
                     /locus_tag="KLH32_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0137755.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="KLH32_000807"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="KLH32_000807"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBE0137756.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="KLH32_000808"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="KLH32_000808"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBE0137757.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KLH32_000809"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KLH32_000809"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBE0137758.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="KLH32_000810"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="KLH32_000810"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBE0137759.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="KLH32_000811"
     CDS             complement(3726..4955)
                     /locus_tag="KLH32_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE0137760.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="KLH32_000812"
     CDS             complement(5019..5459)
                     /locus_tag="KLH32_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE0137761.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="KLH32_000813"
     CDS             complement(5564..6334)
                     /locus_tag="KLH32_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0137762.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="KLH32_000814"
     CDS             complement(6331..7257)
                     /locus_tag="KLH32_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0137763.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="KLH32_000815"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="KLH32_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBE0137764.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KLH32_000816"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KLH32_000816"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBE0137765.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="KLH32_000817"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="KLH32_000817"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBE0137766.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KLH32_000818"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KLH32_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBE0137767.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="KLH32_000819"
     CDS             12964..13311
                     /locus_tag="KLH32_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBE0137768.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="KLH32_000820"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="KLH32_000820"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBE0137769.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="KLH32_000821"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="KLH32_000821"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBE0137770.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="KLH32_000822"
     CDS             complement(15131..15493)
                     /locus_tag="KLH32_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBE0137771.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KLH32_000823"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KLH32_000823"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBE0137772.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="KLH32_000824"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="KLH32_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KLH32_000825"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KLH32_000825"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBE0137773.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24137)
                     /gene="aceF"
                     /locus_tag="KLH32_000826"
     CDS             complement(22293..24137)
                     /gene="aceF"
                     /locus_tag="KLH32_000826"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBE0137774.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGT
                     GRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRI
                     QKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKA
                     VAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSR
                     ELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEP
                     VWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM"
     gene            complement(24152..26815)
                     /gene="aceE"
                     /locus_tag="KLH32_000827"
     CDS             complement(24152..26815)
                     /gene="aceE"
                     /locus_tag="KLH32_000827"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBE0137775.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26976..27740)
                     /gene="pdhR"
                     /locus_tag="KLH32_000828"
     CDS             complement(26976..27740)
                     /gene="pdhR"
                     /locus_tag="KLH32_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBE0137776.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28281..29654
                     /gene="aroP"
                     /locus_tag="KLH32_000829"
     CDS             28281..29654
                     /gene="aroP"
                     /locus_tag="KLH32_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBE0137777.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29697..30551)
                     /gene="ampE"
                     /locus_tag="KLH32_000830"
     CDS             complement(29697..30551)
                     /gene="ampE"
                     /locus_tag="KLH32_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBE0137778.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30548..31099)
                     /gene="ampD"
                     /locus_tag="KLH32_000831"
     CDS             complement(30548..31099)
                     /gene="ampD"
                     /locus_tag="KLH32_000831"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBE0137779.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31187..32080
                     /gene="nadC"
                     /locus_tag="KLH32_000832"
     CDS             31187..32080
                     /gene="nadC"
                     /locus_tag="KLH32_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBE0137780.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32283..32723
                     /gene="ppdD"
                     /locus_tag="KLH32_000833"
     CDS             32283..32723
                     /gene="ppdD"
                     /locus_tag="KLH32_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBE0137781.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32733..33880
                     /gene="tadA"
                     /locus_tag="KLH32_000834"
                     /pseudo
     CDS             32733..33880
                     /gene="tadA"
                     /locus_tag="KLH32_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33870..35072
                     /gene="hofC"
                     /locus_tag="KLH32_000835"
     CDS             33870..35072
                     /gene="hofC"
                     /locus_tag="KLH32_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBE0137782.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35107..36150)
                     /gene="guaC"
                     /locus_tag="KLH32_000836"
     CDS             complement(35107..36150)
                     /gene="guaC"
                     /locus_tag="KLH32_000836"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBE0137783.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36312..36350)
                     /locus_tag="KLH32_000837"
     CDS             complement(36312..36350)
                     /locus_tag="KLH32_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137784.1"
                     /translation="MIIKTLKMSARK"
     gene            36375..36995
                     /gene="coaE"
                     /locus_tag="KLH32_000838"
     CDS             36375..36995
                     /gene="coaE"
                     /locus_tag="KLH32_000838"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBE0137785.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36995..37738
                     /gene="zapD"
                     /locus_tag="KLH32_000839"
     CDS             36995..37738
                     /gene="zapD"
                     /locus_tag="KLH32_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBE0137786.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37748..37945
                     /gene="yacG"
                     /locus_tag="KLH32_000840"
     CDS             37748..37945
                     /gene="yacG"
                     /locus_tag="KLH32_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBE0137787.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38045..38434)
                     /gene="mutT"
                     /locus_tag="KLH32_000841"
     CDS             complement(38045..38434)
                     /gene="mutT"
                     /locus_tag="KLH32_000841"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBE0137788.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38494..41199)
                     /gene="secA"
                     /locus_tag="KLH32_000842"
     CDS             complement(38494..41199)
                     /gene="secA"
                     /locus_tag="KLH32_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBE0137789.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41261..41773)
                     /gene="secM"
                     /locus_tag="KLH32_000843"
     CDS             complement(41261..41773)
                     /gene="secM"
                     /locus_tag="KLH32_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBE0137790.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42004..42921)
                     /gene="lpxC"
                     /locus_tag="KLH32_000844"
     CDS             complement(42004..42921)
                     /gene="lpxC"
                     /locus_tag="KLH32_000844"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBE0137791.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43022..44173)
                     /gene="ftsZ"
                     /locus_tag="KLH32_000845"
     CDS             complement(43022..44173)
                     /gene="ftsZ"
                     /locus_tag="KLH32_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBE0137792.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44234..45496)
                     /gene="ftsA"
                     /locus_tag="KLH32_000846"
     CDS             complement(44234..45496)
                     /gene="ftsA"
                     /locus_tag="KLH32_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBE0137793.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45493..46323)
                     /gene="ftsQ"
                     /locus_tag="KLH32_000847"
     CDS             complement(45493..46323)
                     /gene="ftsQ"
                     /locus_tag="KLH32_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBE0137794.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46325..47245)
                     /gene="ddlB"
                     /locus_tag="KLH32_000848"
     CDS             complement(46325..47245)
                     /gene="ddlB"
                     /locus_tag="KLH32_000848"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE0137795.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPIDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47238..48713)
                     /gene="murC"
                     /locus_tag="KLH32_000849"
     CDS             complement(47238..48713)
                     /gene="murC"
                     /locus_tag="KLH32_000849"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBE0137796.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48767..49834)
                     /gene="murG"
                     /locus_tag="KLH32_000850"
     CDS             complement(48767..49834)
                     /gene="murG"
                     /locus_tag="KLH32_000850"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBE0137797.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49831..51075)
                     /gene="ftsW"
                     /locus_tag="KLH32_000851"
     CDS             complement(49831..51075)
                     /gene="ftsW"
                     /locus_tag="KLH32_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBE0137798.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51075..52391)
                     /gene="murD"
                     /locus_tag="KLH32_000852"
     CDS             complement(51075..52391)
                     /gene="murD"
                     /locus_tag="KLH32_000852"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBE0137799.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52394..53476)
                     /gene="mraY"
                     /locus_tag="KLH32_000853"
     CDS             complement(52394..53476)
                     /gene="mraY"
                     /locus_tag="KLH32_000853"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBE0137800.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53470..54828)
                     /gene="murF"
                     /locus_tag="KLH32_000854"
     CDS             complement(53470..54828)
                     /gene="murF"
                     /locus_tag="KLH32_000854"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBE0137801.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54825..56312)
                     /gene="murE"
                     /locus_tag="KLH32_000855"
     CDS             complement(54825..56312)
                     /gene="murE"
                     /locus_tag="KLH32_000855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBE0137802.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56299..58065)
                     /gene="ftsI"
                     /locus_tag="KLH32_000856"
     CDS             complement(56299..58065)
                     /gene="ftsI"
                     /locus_tag="KLH32_000856"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBE0137803.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58081..58446)
                     /gene="ftsL"
                     /locus_tag="KLH32_000857"
     CDS             complement(58081..58446)
                     /gene="ftsL"
                     /locus_tag="KLH32_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBE0137804.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58443..59384)
                     /gene="rsmH"
                     /locus_tag="KLH32_000858"
     CDS             complement(58443..59384)
                     /gene="rsmH"
                     /locus_tag="KLH32_000858"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBE0137805.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59386..59844)
                     /gene="mraZ"
                     /locus_tag="KLH32_000859"
     CDS             complement(59386..59844)
                     /gene="mraZ"
                     /locus_tag="KLH32_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBE0137806.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16718 c        14836 g        14357 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgaacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagcg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aatttgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg cgccttcaac ttcgaagatc ataatcagcg aaccagtttt cactttatcg
    23341 ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccggaac ttccatagaa
    23401 gctttatcgc cttctacggt aatcagtgac tgttcagcgg caactttgtc gcccactttc
    23461 accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt aacttctttc
    23521 acgccagctg ctggtgcagg ggtcgctgcc ggagtcgctt cctgtttagc cgccggagct
    23581 gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt agacactttg
    23641 tcacccacgt tcactttgat ctctttcacg gtacctgcaa acggagccgg aacttccata
    23701 gaagccttgt cgccttctac ggtgatcagc gactgttcag cttcaacttt atcgccaact
    23761 ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac gttaacgtct
    23821 tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc ttctgcctga
    23881 gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag tgcgccggtc
    23941 tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg cggagatgga
    24001 acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc ttcaactttg
    24061 tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat gtccggtact
    24121 ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta actttatctg
    24181 catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt tcgccacgtt
    24241 tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg aagtggtgac
    24301 gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg cgatagtcgt
    24361 cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg gtagatgcca
    24421 ctgccggcgc gtcgttcatc acctgagcga tatacggaac gcgcggagtt tccagcggat
    24481 gcagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg aaggaggtca
    24541 cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc gcttcacgga
    24601 cgtgacgcag gatggaaccg gagcccagca gctgaacttt acctttgcta ccttcaatgg
    24661 tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc attgccggca
    24721 tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt ttttcaccgt
    24781 acatacgctc cagaccgtca tgcataatga cagcaacttc gtaagcgtaa gccgggtcgt
    24841 aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct tcgtgctgca
    24901 gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag ccacgcgctt
    24961 gctggtctcc agccgcccag cacagatcgc cgatacgctg gaagccgaac atcgagtaat
    25021 agatgtagaa tgggatcatc ggcagattgt tcgtgctgta agaggtcgcc gctgccagcc
    25081 aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct ttctcgtctt
    25141 ctttatagta agcaacctgc tcgcggtcct gcggggtgta ctgctgaccg ttcgggctgt
    25201 aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg tcggcgatga
    25261 tcggtaccag acgatctttg atcgacttgt tcttcagcat cacgttcaga gcacgaacga
    25321 aagcgatagt ggtagagagc tctttgctct gctcttccag cagcgcgccg aagtcttgca
    25381 ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga taaccgtgca
    25441 gtttctgacg ctgagcgtgc agataggtat gctcttcaga accttccggg aaggtgatgt
    25501 acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg cggatgtgac
    25561 gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct tcagccgcgt
    25621 cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg cctttggttt
    25681 cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca cggttcagtg
    25741 cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga tatttaccga
    25801 agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag tcgccgtcaa
    25861 cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc agttcatccc
    25921 aacggctacc ccacatcact ttgatcacgt tccagccagc accttcgaag atgccttcca
    25981 gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc aggttacagt
    26041 tgataacgaa aaccaggtta tccagttttt cacgggtagc gatggtgatc gcacctttgg
    26101 attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt ttagaggtat
    26161 ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata gcaccaatcg
    26221 gacccagacc catagatacg gtcgggaact gccagaattc cggcatcagt ttcgggtgcg
    26281 gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc tgctcctgag
    26341 tcagacgacc ttccaggaaa gcacgggcgt aaacgcccgg ggagatgtgg ccctggaagt
    26401 aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta aagcacacat
    26461 cataaatggt tgcggaagac tggaaggacg ccatgtggcc gcccagttcg aggtcttttt
    26521 tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga atacggcgtt
    26581 ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg ttgatgtagt
    26641 tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca gcaagcagtt
    26701 ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat tcgatcgcct
    26761 ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct gacatgggtt
    26821 attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga actctcgcca
    26881 gaaagctcaa taagacaggt tctacgttta gttaccgcgc ttttatatgc gcttgattta
    26941 caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg ctccagacga
    27001 cgcagagaac gctcacggcg gctctcttca cggcttctgt cgagcaaaat ttcttcgata
    27061 aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc cataatcgct
    27121 tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg cgaatagagc
    27181 aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct tagcagatga
    27241 agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag tacggcgttt
    27301 gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg gagttcacgg
    27361 atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc gataccttcc
    27421 agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga gagcagctcc
    27481 accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc gccaccctgg
    27541 cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa ggagggacgg
    27601 gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc cgggcggaga
    27661 gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca catcggagag ttttggttgg
    27721 cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt caattggtct
    27781 taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt ccatacaggt
    27841 tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata acgtttcaaa
    27901 gttgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa cgattattaa
    27961 actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt aagtcaataa
    28021 aaacgaattt aaattcactc tgcatattga gaggggttga ggctgagctt tacaaacggt
    28081 ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc tgccgcatac
    28141 cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcctt gatgtaaata
    28201 aatcaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag atcaaaaaaa
    28261 caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag ctaaagcgcg
    28321 gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg acagggctat
    28381 tcctaggtag cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca
    28441 ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg gtcgaagaac
    28501 ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg
    28561 cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag ctgactgccg
    28621 tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct gccgccgtat
    28681 tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc gagatggagt
    28741 tctggtttgc cattatcaaa gttattgcgg tagtagcgat gatcatcttc ggcggctggc
    28801 tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg gatcaaggtg
    28861 gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc atgttctcgt
    28921 tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg gagcaaagta
    28981 tcccgaaagc aactaaccag gttatctacc gcatcctgat tttctatatt ggttcgttag
    29041 ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt ccgtttgtgc
    29101 tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc gtggtactga
    29161 ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgtttggtc
    29221 tggcacaaca ggggaacgcg ccaaaagcgc tggcgtctgt cgataaacgc ggcgtaccgg
    29281 ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt aactatcttg
    29341 ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg gtgatcaact
    29401 ggacgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa caaggcgtgg
    29461 taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg
    29521 cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc
    29581 cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg
    29641 taaaagcgca ttaatctctc tacgccctca cccgtacagg gtgagggcaa taatctctac
    29701 accaacgccc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa cgaggttttc
    29761 ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg ttcacgcgcc
    29821 agagagaact gcgccagacg cgttaatacc tgatattgcg aagtatggaa atcacccagc
    29881 gaagcaaacc aggccggtaa cgctttctca ccatgaccga tcaaggcata taccacaccc
    29941 gcaagacgaa ccggcaccca atccagtaca tgaagcactg catcaatgcc ggactgtaaa
    30001 cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa gaacgcatac
    30061 cccatcagcg taacgggtcc ccaggttccc cccacaatca gccagaacag cggtgcaaga
    30121 taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttcg
    30181 tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca
    30241 tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct
    30301 gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac gttgaacaat
    30361 actccctgca atgcgcgtaa cagtaaaaaa gtcacaccca tcgcaataat ggtcatgcct
    30421 aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca
    30481 agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta
    30541 aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa tcaaatgcag
    30601 gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg ttattagcga
    30661 tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga tactgcgcat
    30721 cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa tcattgcagc
    30781 gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg aaaggaacat
    30841 actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc aaatgggcga
    30901 tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat gcgtcgatcc
    30961 acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg
    31021 tttcgtcatc caggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccaaccagcc
    31081 acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag
    31141 tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg
    31201 ctacaaccct gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg
    31261 cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgacat
    31321 tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc acggtgatca cccgcgagaa
    31381 tggcgtattt tgtggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga
    31441 tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc aacccttgtt
    31501 cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt
    31561 gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac tggaaggcac
    31621 caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag ctctgaaata
    31681 cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg ccttcctgat
    31741 caaagaaaac catattattg cctccggctc agtgcgccag gcggtcgaaa aagcgtcctg
    31801 gctgcatccg gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaggc
    31861 cctgaaagcc ggagccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga
    31921 agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa
    31981 aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg cgctaactaa
    32041 acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag atttatccgc
    32101 tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct cgcaaatttc
    32161 ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg ctttcccgtt tcgttttttg
    32221 cccctcgcct gtgctgatgg cgtttgccaa agtagcacca accaaatcaa ggagcgaaac
    32281 agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt ggcatcattg
    32341 ccattttaag ctccattggt attcccgctt atcaaaacta cctgcgcaaa gccgcactca
    32401 ccgacatgct acaaaccttt gtgccttacc gtaccgccgt agagttgtgc gcgctggaac
    32461 atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct accaccaccc
    32521 gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc gctgaccggg caagaaagtc
    32581 tcaatgggct aagcgtcgtc atgacgccag gttgggataa cgcaaacggc gtcaccggct
    32641 gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc gaagatgtct
    32701 tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc acggccctgt
    32761 gcctgcgtta tcagggagtc ttgctggatg ccagcgaaga agtggttcat gttgcggtgg
    32821 tcgatgcccc ctcacatgag ttgctggacg cattgcattt cgctaccacc aaacgtattg
    32881 agatcacctg ctggacgcgc caacaaatgg aaggtcacgc cagtcgcaca caacagacat
    32941 tacccgtagc tgttcaggag aagcatcagc ccaaagcaga attgctgact cgaacgttac
    33001 aatctgcgct ggaacaacgc gcgtctgata ttcatatcga accagcggac aatgcctacc
    33061 gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca ccggatgccg
    33121 gagtcgcatt aacagccaga ttaaaagtgc tgggaaacct ggatattgcg gaacatcgcc
    33181 tgccgcagga cgggcaattc actgtcgaac tggcaggaaa caccgtctca tttcgtattg
    33241 cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag caggtgagcc
    33301 aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac tttgctcatg
    33361 ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagt ggcaaaacgg
    33421 tcacctggtg ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca
    33481 gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg
    33541 tctggtacgt aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc
    33601 agacactgta tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta
    33661 ccacggtttt tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg
    33721 ccagcttatt tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg
    33781 tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga
    33841 agagttaatc cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg
    33901 gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac
    33961 tgcttatggc actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca
    34021 attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac
    34081 ttaaagccgg gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta
    34141 agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt
    34201 ccaatgcctt attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca
    34261 cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat
    34321 cccagcgtca gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag
    34381 cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta
    34441 tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag
    34501 actttagtgg cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca
    34561 ataagttgct gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca
    34621 tcccgattat gggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg
    34681 cgctgacaca aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa
    34741 tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc
    34801 atcccatctg gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag
    34861 tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc
    34921 atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc
    34981 tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc
    35041 atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt
    35101 gggagattac aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt
    35161 gcgcttggtc agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc
    35221 gcccaaaata tcacgcgcgg tattttcaac ctggcctcgc agcggcagct taacggtttt
    35281 accttctgct gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga
    35341 gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct
    35401 ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc
    35461 tttcgccaca tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc
    35521 gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt
    35581 gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga
    35641 gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt
    35701 cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc
    35761 cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc
    35821 gaaatccgca tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga
    35881 attgttgata aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat
    35941 atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc
    36001 gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag
    36061 ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa
    36121 acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag
    36181 ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc
    36241 gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg
    36301 tttcatcaat ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc
    36361 cgggaatata aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga
    36421 gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg
    36481 cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg
    36541 aaaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg
    36601 ccaacccgga agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga
    36661 cgcaacacca gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctgg
    36721 tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa
    36781 cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc
    36841 ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata
    36901 acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc
    36961 ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca
    37021 tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac
    37081 cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga
    37141 attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg
    37201 gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga
    37261 agcattaatt cagcagttaa aagcggcggt gagcgtatta atttccgcgc cgcgtatcgg
    37321 gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg
    37381 cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca
    37441 gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac
    37501 catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg
    37561 tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc
    37621 acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct
    37681 ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg
    37741 agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg
    37801 gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg
    37861 agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga
    37921 ctggagcgaa gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac
    37981 gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc
    38041 cgacctacag acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg
    38101 cattaagacc gaccagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc
    38161 cttcccaact ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt
    38221 ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac
    38281 gcaccaccgc ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt
    38341 tattcgccat gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc
    38401 gaataatacc taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca
    38461 gaatcctgcg ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt
    38521 gtatttttta ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt
    38581 ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat
    38641 ttgtgctaaa cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg
    38701 catacgtacc tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat
    38761 cgctgcaaac atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata
    38821 gccacgcagg tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag
    38881 ggagtcaagc gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac
    38941 cacttcttct ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt
    39001 ctcttcatgc agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa
    39061 atcgttcttc agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg
    39121 cggaatgtag gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc
    39181 gctcacatcg ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc
    39241 gttagccacg tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac
    39301 tttacgctgg gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg
    39361 cttcataccc agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag
    39421 cgcatcttcc atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg
    39481 accgcgcaac tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag
    39541 gccacctgct tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat
    39601 ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac
    39661 aatatctgta ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc
    39721 ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat
    39781 accggctttg gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac
    39841 cagcaccggc tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt
    39901 ttccgcttca gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt
    39961 cggaacaacg acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc
    40021 agtaccggtc atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga
    40081 agccagcgtt tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg
    40141 cagaccatcg gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat
    40201 aacttcacca tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag
    40261 cgccgccgtt acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc
    40321 atccatgatg ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag
    40381 gttcacctgg cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga
    40441 gtcttctttt tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc
    40501 cgagctgtct tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat
    40561 ggagtccact tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct
    40621 gaacgccatg ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat
    40681 gtcagctgcg taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac
    40741 agtcaggcca aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta
    40801 gtcgttgacg gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag
    40861 cgttgcggtc agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag
    40921 aaccataccg ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc
    40981 ctcacgcacc acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag
    41041 acgcgcacga aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc
    41101 cggttccatg gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt
    41161 acgactaccg aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg
    41221 cgttacggag ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc
    41281 cagcacggat gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg
    41341 taaaatgcgc ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca
    41401 gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag
    41461 caacgggcag tgtttgcggt gccattgcga aagaaagatg acggattacc gtacgaatag
    41521 catgttgatg ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca
    41581 gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt
    41641 ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga
    41701 ccatccctaa taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca
    41761 gtattccact cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct
    41821 tatcattaaa gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta
    41881 agaaaacagc gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg
    41941 ttcacctggc cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg
    42001 tcgttatgcc agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg
    42061 gaaggtcaca tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt
    42121 cagtgcatga ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa
    42181 caagtcaccg atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc
    42241 gtcttcgttc agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag
    42301 gcacaaacca cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg
    42361 gctgatctgg cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga
    42421 atcaatagcc ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt
    42481 aaattcagcc cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt
    42541 tttggcgcag ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc
    42601 gctgccgtcc atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc
    42661 caggcccgcg agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt
    42721 gaccagacac gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac
    42781 cggtggattc aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa
    42841 cgtcagggtg actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt
    42901 aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta
    42961 ctatacattc ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt
    43021 cttaatcagc ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag
    43081 tttgcggcgc attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag
    43141 ccatcccatg ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca
    43201 gagtgatttc aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct
    43261 cgtcattcat atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa
    43321 atgcacggat ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg
    43381 tgatgttaac cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag
    43441 agatagccat ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca
    43501 tcattgcgta gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca
    43561 aacccggacg agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg
    43621 ctgcgccaaa cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt
    43681 tcgggatagt gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga
    43741 atgccatacg cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga
    43801 tacccaaatc ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac
    43861 ccatacccgc agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc
    43921 gatcctcatc agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg
    43981 tgataccgct accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat
    44041 cggtatttac cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag
    44101 cattaccgcc gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca
    44161 ttggttcaaa catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc
    44221 ggcctcataa aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc
    44281 caactgatgc tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc
    44341 catagtgaag caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac
    44401 cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg
    44461 ctgcaagacc ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat
    44521 gtttaacccc ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt
    44581 tgagcaactc ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc
    44641 gcggcggacg accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac
    44701 ccagcgcaca accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg
    44761 cgtaagcgat atcactggtc acgacattgc cagcataagg aattaccttt gtgtggcgca
    44821 atgccccacc ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga
    44881 cacccagttc acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata
    44941 tcagttggtc aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat
    45001 cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg
    45061 gattcttgat cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac
    45121 gatgctcatc gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt
    45181 gcgtcacttc ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct
    45241 taccagaaag cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt
    45301 caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca
    45361 taccacgtga cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt
    45421 cccctactaa agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt
    45481 ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc
    45541 tggcggcaag ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct
    45601 aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg
    45661 aaccaaacgt ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt
    45721 caactgccag gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc
    45781 cagcatctgc cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc
    45841 atacagcatt ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc
    45901 ttccgcgtct accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg
    45961 aatcttcaat tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg
    46021 ttgttctatt tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc
    46081 caatgccagg atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa
    46141 caccagcttt gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc
    46201 gctcaccaac actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc
    46261 attattgcgg cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga
    46321 catattagtc cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct
    46381 gacgtgccgc catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca
    46441 gcagataaaa ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac
    46501 ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgcttcca
    46561 gacctgcggg gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga
    46621 aggttccgga cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg
    46681 tgaactccgg cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca
    46741 atcttaatgc atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac
    46801 cttcgcggct cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct
    46861 tatcgctcag gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta
    46921 aaccggcacc ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca
    46981 tcactccgct gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat
    47041 cttcaccgcc gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt
    47101 gcgtcacgtc gacttctttc gggtcgatag gatacgcatc aataccgcct tcacgcagcc
    47161 cggctaacac cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca
    47221 acaggaccgc gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg
    47281 atttcagcta aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg
    47341 tcgttaccgg ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga
    47401 atgggatcaa ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc
    47461 ggaattggcg cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc
    47521 agcacattgg cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac
    47581 agcattacca ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact
    47641 tccgtcgggt ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact
    47701 ggctccagcg ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc
    47761 agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct
    47821 gcggcgttca gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg
    47881 tcctgacgca gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc
    47941 acgtcggcat cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat
    48001 tcgcggatca ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc
    48061 agaaaattaa taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg
    48121 tcggcttcga tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca
    48181 ctctcatctg cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc
    48241 gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg
    48301 gaaaccatcg cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga
    48361 aaacgcatta actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga
    48421 gctgcgacaa tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca
    48481 tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc
    48541 tgcgtgaccg gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga
    48601 acttcggcaa taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg
    48661 cgacgcattt cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac
    48721 gccattaact tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag
    48781 cccggctcac ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc
    48841 gttctgccat ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat
    48901 ccacgctaag ctgtggctgc tcaataattt tggctgcgcc cgctttttcc agcggtagcg
    48961 cattccagta ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg
    49021 ccgcggcgat ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg
    49081 catacgccgc cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt
    49141 cggcatacgc ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga
    49201 ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct
    49261 gggaaccacc cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg
    49321 gcaacggcag cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga
    49381 aagcgcctgg aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta
    49441 agcccgcaat accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca
    49501 gaccacctgg acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt
    49561 tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca gctatcagtg
    49621 cttttatacc ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt
    49681 ttggcactaa gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct
    49741 gagccattag atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg
    49801 ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc
    49861 tttctccaga cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat
    49921 cagtaagctc gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat
    49981 ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca
    50041 ggcgagaaaa ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc
    50101 acgaaaagcg acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc
    50161 ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg
    50221 taccgaatta cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg
    50281 cgttaactga tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg
    50341 gcggatacgg tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat
    50401 aatggcaatg aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac
    50461 aaacaacacc accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag
    50521 aatcacgccc atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg
    50581 caccagatag ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat
    50641 acgcagcaaa ccgagatcga tccaacgcga tgccccttta acagcgctac ccactaccag
    50701 cacgatcatc agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa
    50761 ctccatcggc agacgcagcg taatgatcgc cagaataaac gccaaaatca gatagacacc
    50821 atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc
    50881 cgaggtcacc atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg
    50941 atcgtacatg atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag
    51001 cgccgtggag atccagacca ggatactgaa tcctggcagg cgcggcattt tcaggcgagg
    51061 gagagataaa cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt
    51121 tcaaagttct tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc
    51181 ggctgaacac gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc
    51241 acttccgggc gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg
    51301 ttatcgccat tcaggtaacg caccagtggg ctaaagtccg ccgatttacc atcgccgccc
    51361 agcaacaaat gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg
    51421 acgttggtcg cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca
    51481 aagcgatgcg gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac
    51541 cctgcggcat ctgccagcgc cagcgccacc agcgcattgg tgtagttatg ctgcccggaa
    51601 agtttcatct ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc
    51661 tgctgatgat tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc
    51721 gcaccgcgaa tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt
    51781 tcgtaaatgc gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata
    51841 tgatcttcag tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc
    51901 agctggaagc tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac
    51961 gcaggcaggc caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca
    52021 cccactagcg tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct
    52081 tgtgcttcgc gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca
    52141 gcgcttaagg atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc
    52201 atcagccatt catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc
    52261 aggccaggcg gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa
    52321 aagtccacgc aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccctgg
    52381 taatcagcca tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg
    52441 aaataatcca gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt
    52501 gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca
    52561 ggatgacaga aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct
    52621 gacgtagcag tacggcgata atgcctaacg caccacctaa cgccagcgaa ccgacatcgc
    52681 ccataaagac ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta
    52741 tcgcggtaca gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc
    52801 tggcaaagtt catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata
    52861 cggtcggcat aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac
    52921 ccacaatgac gaagtaagcc agcagaatgt agaacagccc cagttgcggc atcacatctt
    52981 taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga
    53041 aagcaacacc cagcgcaatg accgacatcc agaaatattt ccaacgagcg atcaaccctt
    53101 tggtgtcttt acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta
    53161 ccagcaccac caacacgcac cagacgtacg gattggacgg gtaagcccac agcagcacgg
    53221 agatcacaat cgctgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga
    53281 agtgtgattc aggaccgtcg ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag
    53341 caatcatgcg cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg
    53401 cgcgaaacgt cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg
    53461 ccagccaaac taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcagc
    53521 actacgtgaa cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag
    53581 acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat
    53641 agcatggctt tgtttaccca tgcttaacac gcggtcaata ccagcagctt ttgccgcctc
    53701 acccacctgt acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac
    53761 cagcacgcgg tagcccggca tttcagccag tacctgaaca gctgcggtca ttgaaccgac
    53821 attggcgttg taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag
    53881 acggccagga acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc
    53941 cacggacatg gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa
    54001 cggcagcaga acatcgacgc taccggttgg ggtttgtagg gtaaattcca taccgtgcga
    54061 ggtcacatgg atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca
    54121 cactttgcgt gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat
    54181 aatggcgata ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc
    54241 cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc
    54301 cgggcgagtc agactcacag tccaggcaat ctcgccctga tggttcgcgc caagttcaat
    54361 aactgcgtaa tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt
    54421 gttgagattg cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat
    54481 ctctttaacg gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg
    54541 ctggcgaacc catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg
    54601 cggcaggtcg atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc
    54661 ctggtcggca aaatcgtggg catcaaaacg ttcacctttc agggcaacaa acaggcagcc
    54721 cggcgtcagt tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag
    54781 ttcaccgttg agaatatcgg taagttggct aagggttacg ctaatcatgc aatccccccc
    54841 agcagacgcg ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg
    54901 taatcctcat ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg
    54961 gcgcaagtca ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt
    55021 cccgccagaa tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc
    55081 accaccgcca cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta
    55141 tcgcgatcgc caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc
    55201 gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt
    55261 ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt
    55321 ttcagcagat cagccagtgg atagcccagc gccagcagtg ttgccagcgc gagcaacagg
    55381 ttgctgacgt taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccagcttgaa
    55441 ctaaagcgaa tggtcgcgcc gctgtcgtga tagttcactt cggtcgcttt caaccagcgt
    55501 ccgtgacagt tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt
    55561 gccagccagc ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt
    55621 tcagaataaa gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg
    55681 tgatcgcggc ttaagttggt aaagaccgac gccggaaatt tcaatgccgc cacacggtgc
    55741 tgtaccagcc cgtgggagga aacttccatt gcgcaaaaag tcgcgccctg atccaccagc
    55801 cccgccagct catgctgaac atcaactgcc gaaccggtag tattttctgt cgggatcact
    55861 ttccccagca gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg
    55921 ctccactgcg ccagaagctg ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg
    55981 agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg
    56041 ttgagctggc tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc
    56101 gcctcatctt tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata
    56161 tatcgacgcc cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc
    56221 acacagctgt cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga
    56281 gcaagaaggt cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa
    56341 ttcattttta tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc
    56401 gcccatgatg gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg
    56461 cggatcgttg ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc
    56521 ggtataagca atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc
    56581 ggttttaatg gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcctggtag
    56641 cgccacgctt tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc
    56701 accgggaacc ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc
    56761 gatagttgcg tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga
    56821 gaaggtggcc ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc
    56881 tccgaccaac cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa
    56941 cgctgaggac ggcatcgtta acgccagctt ggaaacaccg acgttactcg acttctgtaa
    57001 taccccggtc agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat
    57061 tcgataagga atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt
    57121 catcaccacc atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt
    57181 acgcatcgac tctttcggcg tgccgctcag attgttaggg ttgtatgacg ggctgttagc
    57241 catcgccagc acttcacctg tgttgacatc caccagcacg gcgctaccag attcagcctt
    57301 gttaaaggcc accgcgttgt tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat
    57361 actcagcgcc aggttgtgcg ctgcctggct gtcagtagaa gaaatatctt caattacgcg
    57421 accatagcgg tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa
    57481 actcttctca acgccctcaa tcccttgact atcgacgtta gtaaagccga tgaggtgagc
    57541 agtcacttcg ccggacggat agtaacggcg agactcttca cgcagatgaa tccccggcag
    57601 tttcagtttt ttgatgtagt ccgccatgtc agagtttacc tgacgcgcca gataaataaa
    57661 gcgccctttc gggttggcgt taatgcgggc agaaagctga tccagcggaa tattgagcgc
    57721 gttagccagc gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt
    57781 cgggtcagcc caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc
    57841 agtaatcatg ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc
    57901 tttcaccagc atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc
    57961 cagcgccagg agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg
    58021 ttcttcctga cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc
    58081 ttatttttgc actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc
    58141 cgtggcgatc ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt
    58201 gcgccattca atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag
    58261 taaacgggta tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa
    58321 caggcagagt ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc
    58381 atggcgctcg tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct
    58441 gatcatgcgt tcgtcctctc tgcaatacgc agaactgaac tacgggcacg agggttctca
    58501 gccacctctt cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt
    58561 tttttgagct gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt
    58621 tcacgcataa aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc
    58681 cgcccacccg gggccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt
    58741 tcactgttta cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta
    58801 tctttcaccg gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc
    58861 tgttcgcggt tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaatg ctcttcacca
    58921 taggttttca atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct
    58981 gactgcccac gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag
    59041 ccgcgttcag catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg
    59101 atcttgccaa taagatcgcg ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg
    59161 atgatggaga agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga
    59221 tcgatcgcca gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca
    59281 ccgcgaccaa aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg
    59341 gcttcatcca gcagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc
    59401 ctgcagtcgc tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac
    59461 ctgttgatgc caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat
    59521 cacttctttt gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc
    59581 gccatccatc tgacattcgc tggcatgacc taacagtagg cgctgcacac ggcgctcaac
    59641 cgggttcatg ctcaacagac gcgataattt ttgctcgata atttcccatt caggcagggg
    59701 gtaaagcagc aggcacgggt gatgaatgtc aatggtgcaa accatttgac cggcagcgtt
    59761 ctcaagcagc tgttcccgat aacgggtagg cactgataag cgccctttgc tgtcgagatt
    59821 gactaacgtt gctccccgga acatgccagc ctcacccctt attcccactt tgccccacaa
    59881 tttcccactg aaaggagttt acggagcgga ggaaaagctt gtcaagccag tcacaaggca
    59941 taccgaggta aaaaacccga tgtttacggc taatatcagc gttgtttatt ttacgtccaa
    60001 cgtgcgaagc aaattaacgt tatgaatagc tgtaagaaaa aatccctcat cgtcatcact
    60061 gttttaaaat gactcactat tttccacagc aaatataaag tgtcagtttg cgacgcgagc
    60121 ggcattttag gacatatctt ccccggttaa cagtccttgt tacgtctgtg tggcgcttga
    60181 tgccagcggg tttgcgcaaa agcaaagaaa agtgtttgtt aaatcggcaa ttcatcgccg
    60241 catgtaacaa aataatgcaa aaacgcgctg tcgatatcac ttaattattg tgaatttcag
    60301 ctggtgagtt aattaacggg cgggtttaaa accatttatc aataatagtc aggaatagtc
    60361 ttatttactt taaggatatt gat
//
LOCUS       DAEEDS010000013        59845 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEEDS010000013 DAEEDS010000000
VERSION     DAEEDS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59845
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.013"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="KLH32_000860"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="KLH32_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBE0137807.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="KLH32_000861"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="KLH32_000861"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBE0137808.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="KLH32_000862"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="KLH32_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBE0137809.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KLH32_000863"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KLH32_000863"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBE0137810.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KLH32_000864"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KLH32_000864"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBE0137811.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="KLH32_000865"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="KLH32_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBE0137812.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="KLH32_000866"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="KLH32_000866"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBE0137813.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="KLH32_000867"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="KLH32_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="KLH32_000868"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="KLH32_000868"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBE0137814.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="KLH32_000869"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="KLH32_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBE0137815.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KLH32_000870"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KLH32_000870"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBE0137816.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="KLH32_000871"
     CDS             complement(13538..13933)
                     /locus_tag="KLH32_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBE0137817.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KLH32_000872"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KLH32_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBE0137818.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="KLH32_000873"
     CDS             complement(15220..15447)
                     /locus_tag="KLH32_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBE0137819.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="KLH32_000874"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="KLH32_000874"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBE0137820.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="KLH32_000875"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="KLH32_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBE0137821.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KLH32_000876"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KLH32_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBE0137822.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KLH32_000877"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KLH32_000877"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBE0137823.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="KLH32_000878"
     tRNA            19689..19765
                     /locus_tag="KLH32_000878"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19799..19875
                     /locus_tag="KLH32_000879"
     tRNA            19799..19875
                     /locus_tag="KLH32_000879"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19833..19835,aa:Met,seq:cat)
     gene            complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KLH32_000880"
     CDS             complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KLH32_000880"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBE0137824.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21435..22766
                     /gene="argA"
                     /locus_tag="KLH32_000881"
     CDS             21435..22766
                     /gene="argA"
                     /locus_tag="KLH32_000881"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBE0137825.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KLH32_000882"
     CDS             complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KLH32_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBE0137826.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KLH32_000883"
     CDS             complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KLH32_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBE0137827.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KLH32_000884"
                     /pseudo
     CDS             complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KLH32_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
     gene            complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KLH32_000885"
     CDS             complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KLH32_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBE0137828.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34634..34966)
                     /locus_tag="KLH32_000886"
     CDS             complement(34634..34966)
                     /locus_tag="KLH32_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBE0137829.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34951..35358)
                     /locus_tag="KLH32_000887"
     CDS             complement(34951..35358)
                     /locus_tag="KLH32_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBE0137830.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35355..35918)
                     /locus_tag="KLH32_000888"
     CDS             complement(35355..35918)
                     /locus_tag="KLH32_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBE0137831.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35909..36379)
                     /locus_tag="KLH32_000889"
                     /pseudo
     CDS             complement(35909..36379)
                     /locus_tag="KLH32_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KLH32_000890"
     CDS             complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KLH32_000890"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBE0137832.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KLH32_000891"
     CDS             complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KLH32_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBE0137833.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KLH32_000892"
     CDS             complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KLH32_000892"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0137834.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KLH32_000893"
     CDS             complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KLH32_000893"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBE0137835.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41864..42553
                     /gene="mutH"
                     /locus_tag="KLH32_000894"
     CDS             41864..42553
                     /gene="mutH"
                     /locus_tag="KLH32_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBE0137836.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42622..43335
                     /locus_tag="KLH32_000895"
     CDS             42622..43335
                     /locus_tag="KLH32_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE0137837.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43473..43691
                     /gene="ygdR"
                     /locus_tag="KLH32_000896"
     CDS             43473..43691
                     /gene="ygdR"
                     /locus_tag="KLH32_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBE0137838.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43799..44839
                     /locus_tag="KLH32_000897"
     CDS             43799..44839
                     /locus_tag="KLH32_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBE0137839.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KLH32_000898"
     CDS             complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KLH32_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBE0137840.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KLH32_000899"
     CDS             complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KLH32_000899"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBE0137841.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48800..49831
                     /gene="galR"
                     /locus_tag="KLH32_000900"
     CDS             48800..49831
                     /gene="galR"
                     /locus_tag="KLH32_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBE0137842.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KLH32_000901"
     CDS             complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KLH32_000901"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBE0137843.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51222..52157
                     /gene="lysR"
                     /locus_tag="KLH32_000902"
     CDS             51222..52157
                     /gene="lysR"
                     /locus_tag="KLH32_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBE0137844.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KLH32_000903"
     CDS             complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KLH32_000903"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBE0137845.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KLH32_000904"
     CDS             complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KLH32_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBE0137846.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KLH32_000905"
     CDS             complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KLH32_000905"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBE0137847.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KLH32_000906"
     CDS             complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KLH32_000906"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBE0137848.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56612..57793)
                     /locus_tag="KLH32_000907"
     CDS             complement(56612..57793)
                     /locus_tag="KLH32_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBE0137849.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58048..59277
                     /locus_tag="KLH32_000908"
                     /pseudo
     CDS             58048..59277
                     /locus_tag="KLH32_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14777 a        16053 c        14573 g        14442 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag tacggtgacg
    19801 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19861 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgatctccca
    19921 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt tgccccatcg
    19981 gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    20041 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    20101 ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc
    20161 ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct gtcagcaata
    20221 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20281 acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg
    20341 gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg actggtaaag
    20401 gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc
    20461 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20521 tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt ttcgcgcgtt
    20581 ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca
    20641 atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg cggcacctgc
    20701 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20761 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20821 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg tacggtttgc
    20881 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20941 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttca
    21001 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    21061 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    21121 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21181 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21241 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21301 acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa
    21361 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca aataatcatg
    21421 caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga ttccgccatt
    21481 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21541 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21601 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21661 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21721 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcaatgagtc
    21781 tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21841 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21901 ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta atggggccgg
    21961 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    22021 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    22081 atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag
    22141 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22201 aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22261 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22321 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22381 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22441 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22501 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22561 cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca
    22621 aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga tttaccccgg
    22681 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22741 aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc aggcgtaaca
    22801 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22861 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22921 cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22981 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    23041 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    23101 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    23161 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc
    23221 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgttc
    23281 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg
    23341 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23401 ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg
    23461 taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23521 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
    23581 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc
    23641 cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg taaagcccgc
    23701 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23761 acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca gtctcgacat
    23821 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23881 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
    23941 gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    24001 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    24061 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg ccacggtggt
    24121 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24181 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24241 cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga agcgcgctac
    24301 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24361 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24421 tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct cgttattttc
    24481 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24541 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24601 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24661 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24721 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24781 ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24841 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24901 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24961 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    25021 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    25081 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    25141 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25201 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25261 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25321 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25381 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25441 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25501 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25561 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25621 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25681 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25741 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25801 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcattaaga
    25861 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25921 ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt atggatcgtg
    25981 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
    26041 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    26101 gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    26161 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26221 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
    26281 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
    26341 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26401 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26461 acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg
    26521 cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26581 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26641 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26701 atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccggctgat
    26761 ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg gctgaaaagc
    26821 ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg
    26881 gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26941 atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    27001 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
    27061 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    27121 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27181 actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat cgacaatggt
    27241 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggcc
    27301 ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa
    27361 ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc ctgattgcta
    27421 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27481 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27541 gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc ttgcagataa
    27601 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct
    27661 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27721 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
    27781 aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc atccatctgc
    27841 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27901 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27961 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    28021 agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    28081 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    28141 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28201 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28261 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc
    28321 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28381 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28441 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28501 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28561 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28621 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28681 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28741 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28801 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28861 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28921 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28981 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    29041 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    29101 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    29161 ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29221 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca taaggccgtt
    29281 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tagttgctta actgatcaag
    29341 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29401 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    29461 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcgta
    29521 tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa gctctggcaa
    29581 agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt
    29641 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    29701 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt
    29761 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat
    29821 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29881 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29941 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaagccttt
    30001 atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    30061 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg
    30121 gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga acttcgctga
    30181 gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg
    30241 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30301 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30361 tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct cgtggaaatc
    30421 tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc
    30481 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30541 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc
    30601 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    30661 gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30721 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30781 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30841 atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg cctgcggatc
    30901 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30961 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    31021 tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    31081 cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc aaatcatctg
    31141 ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga
    31201 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    31261 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31321 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    31381 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31441 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31501 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    31561 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca ccgtctttgc
    31621 gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31681 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31741 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31801 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31861 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31921 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31981 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    32041 cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    32101 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    32161 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32221 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32281 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32341 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32401 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32461 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32521 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32581 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32641 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgtgcca
    32701 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32761 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32821 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32881 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32941 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    33001 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    33061 gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    33121 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33181 ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag
    33241 gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc gcacgacggt
    33301 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact gcctgaataa
    33361 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33421 gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc
    33481 tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33541 tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt tccagaatgt
    33601 cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    33661 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc ccccatgagg
    33721 ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt ccggcatttt
    33781 cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgagtca
    33841 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33901 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33961 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    34021 tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc cagcgcggtt
    34081 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc caggcctgtg
    34141 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat
    34201 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34261 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccagcaatt
    34321 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34381 ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34441 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34501 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34561 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34621 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34681 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34741 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34801 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat gttcgctgga
    34861 tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc gcaaccatta
    34921 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    34981 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    35041 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    35101 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    35161 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    35221 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    35281 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    35341 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    35401 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    35461 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    35521 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    35581 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    35641 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    35701 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    35761 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    35821 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    35881 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    35941 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    36001 agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc gaaggtgtca
    36061 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    36121 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    36181 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    36241 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    36301 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    36361 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    36421 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    36481 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    36541 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    36601 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    36661 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    36721 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    36781 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    36841 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    36901 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    36961 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    37021 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    37081 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    37141 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    37201 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    37261 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    37321 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    37381 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    37441 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    37501 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    37561 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    37621 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    37681 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    37741 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    37801 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    37861 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    37921 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    37981 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    38041 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    38101 gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    38161 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    38221 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    38281 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    38341 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    38401 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    38461 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    38521 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    38581 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    38641 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    38701 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    38761 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    38821 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    38881 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    38941 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    39001 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    39061 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    39121 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    39181 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    39241 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    39301 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    39361 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    39421 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    39481 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    39541 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct cgcacgacaa
    39601 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    39661 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    39721 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    39781 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    39841 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    39901 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    39961 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    40021 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    40081 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    40141 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    40201 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    40261 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    40321 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    40381 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    40441 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    40501 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    40561 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    40621 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    40681 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    40741 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    40801 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    40861 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    40921 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    40981 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    41041 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    41101 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    41161 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    41221 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    41281 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    41341 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    41401 cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca atgcattaca
    41461 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    41521 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    41581 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    41641 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    41701 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    41761 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    41821 cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    41881 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    41941 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    42001 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    42061 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    42121 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    42181 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    42241 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    42301 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    42361 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    42421 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    42481 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    42541 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    42601 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    42661 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    42721 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    42781 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    42841 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    42901 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    42961 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    43021 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    43081 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    43141 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    43201 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    43261 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    43321 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    43381 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    43441 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    43501 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    43561 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    43621 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    43681 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    43741 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    43801 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    43861 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    43921 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    43981 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    44041 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    44101 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    44161 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    44221 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    44281 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    44341 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    44401 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    44461 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    44521 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    44581 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    44641 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct gtcaaccgtt
    44701 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    44761 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    44821 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    44881 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    44941 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    45001 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    45061 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    45121 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    45181 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    45241 cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt tgagataggt
    45301 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    45361 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    45421 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    45481 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    45541 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    45601 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    45661 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    45721 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    45781 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    45841 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    45901 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca gagtggcaaa
    45961 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    46021 cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt tccgcttcgt
    46081 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    46141 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    46201 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    46261 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    46321 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    46381 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    46441 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    46501 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    46561 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    46621 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    46681 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    46741 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    46801 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    46861 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    46921 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    46981 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    47041 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    47101 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    47161 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    47221 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    47281 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    47341 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    47401 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    47461 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    47521 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    47581 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    47641 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    47701 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    47761 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    47821 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    47881 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    47941 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    48001 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    48061 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    48121 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    48181 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    48241 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    48301 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    48361 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    48421 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    48481 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    48541 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    48601 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    48661 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    48721 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    48781 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    48841 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    48901 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    48961 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    49021 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    49081 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    49141 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    49201 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    49261 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    49321 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    49381 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    49441 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    49501 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    49561 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    49621 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    49681 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    49741 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    49801 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    49861 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    49921 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    49981 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    50041 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    50101 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    50161 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    50221 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    50281 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    50341 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    50401 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    50461 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    50521 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    50581 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    50641 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    50701 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    50761 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    50821 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    50881 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    50941 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    51001 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    51061 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    51121 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    51181 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    51241 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    51301 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    51361 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    51421 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    51481 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    51541 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    51601 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    51661 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    51721 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    51781 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    51841 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    51901 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    51961 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    52021 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    52081 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    52141 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    52201 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    52261 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    52321 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    52381 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    52441 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    52501 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    52561 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    52621 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    52681 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    52741 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    52801 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    52861 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    52921 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    52981 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    53041 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    53101 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    53161 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    53221 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    53281 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    53341 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    53401 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    53461 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    53521 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    53581 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    53641 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    53701 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    53761 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    53821 gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact gaacgctgta
    53881 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    53941 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    54001 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    54061 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    54121 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    54181 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    54241 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    54301 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    54361 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    54421 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    54481 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    54541 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    54601 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    54661 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    54721 ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    54781 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    54841 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    54901 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    54961 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    55021 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    55081 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    55141 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    55201 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    55261 gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc tcgatggttt
    55321 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    55381 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    55441 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    55501 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    55561 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    55621 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    55681 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    55741 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    55801 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    55861 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    55921 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    55981 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    56041 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    56101 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    56161 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    56221 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    56281 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    56341 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    56401 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    56461 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    56521 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    56581 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    56641 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    56701 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    56761 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    56821 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    56881 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    56941 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    57001 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    57061 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    57121 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    57181 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    57241 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    57301 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    57361 gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg ctatcagtca
    57421 gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata tcagcctcgc
    57481 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    57541 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    57601 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    57661 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    57721 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    57781 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    57841 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    57901 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    57961 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    58021 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    58081 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    58141 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    58201 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    58261 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    58321 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    58381 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    58441 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg
    58501 gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
    58561 gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt
    58621 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
    58681 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
    58741 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
    58801 tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
    58861 ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt
    58921 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
    58981 gccacagagg tcgtcaaaac aacactacaa caggttggtg taaagaaaag tcgtgcattt
    59041 aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac
    59101 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
    59161 atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga
    59221 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat
    59281 ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca
    59341 tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa
    59401 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
    59461 caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
    59521 tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta
    59581 ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg
    59641 caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa
    59701 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
    59761 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
    59821 ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAEEDS010000014        58520 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEEDS010000014 DAEEDS010000000
VERSION     DAEEDS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58520
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.014"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..1142)
                     /locus_tag="KLH32_000909"
                     /pseudo
     CDS             complement(1..1142)
                     /locus_tag="KLH32_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(1354..1701)
                     /gene="pspE"
                     /locus_tag="KLH32_000910"
     CDS             complement(1354..1701)
                     /gene="pspE"
                     /locus_tag="KLH32_000910"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBE0137850.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1744..1965)
                     /gene="pspD"
                     /locus_tag="KLH32_000911"
     CDS             complement(1744..1965)
                     /gene="pspD"
                     /locus_tag="KLH32_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBE0137851.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1974..2333)
                     /gene="pspC"
                     /locus_tag="KLH32_000912"
     CDS             complement(1974..2333)
                     /gene="pspC"
                     /locus_tag="KLH32_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBE0137852.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2333..2557)
                     /gene="pspB"
                     /locus_tag="KLH32_000913"
     CDS             complement(2333..2557)
                     /gene="pspB"
                     /locus_tag="KLH32_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBE0137853.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2611..3279)
                     /gene="pspA"
                     /locus_tag="KLH32_000914"
     CDS             complement(2611..3279)
                     /gene="pspA"
                     /locus_tag="KLH32_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBE0137854.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLRETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3446..4423
                     /gene="pspF"
                     /locus_tag="KLH32_000915"
     CDS             3446..4423
                     /gene="pspF"
                     /locus_tag="KLH32_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBE0137855.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4543..5808)
                     /gene="puuE"
                     /locus_tag="KLH32_000916"
     CDS             complement(4543..5808)
                     /gene="puuE"
                     /locus_tag="KLH32_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBE0137856.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5846..7126)
                     /gene="puuB"
                     /locus_tag="KLH32_000917"
     CDS             complement(5846..7126)
                     /gene="puuB"
                     /locus_tag="KLH32_000917"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBE0137857.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7128..8615)
                     /gene="puuC"
                     /locus_tag="KLH32_000918"
     CDS             complement(7128..8615)
                     /gene="puuC"
                     /locus_tag="KLH32_000918"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBE0137858.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8765..9322)
                     /gene="puuR"
                     /locus_tag="KLH32_000919"
     CDS             complement(8765..9322)
                     /gene="puuR"
                     /locus_tag="KLH32_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBE0137859.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9349..10113)
                     /gene="puuD"
                     /locus_tag="KLH32_000920"
     CDS             complement(9349..10113)
                     /gene="puuD"
                     /locus_tag="KLH32_000920"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBE0137860.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10325..11743
                     /gene="puuA"
                     /locus_tag="KLH32_000921"
     CDS             10325..11743
                     /gene="puuA"
                     /locus_tag="KLH32_000921"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBE0137861.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11892..12056
                     /locus_tag="KLH32_000922"
     CDS             11892..12056
                     /locus_tag="KLH32_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137862.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12046..13353
                     /gene="puuP"
                     /locus_tag="KLH32_000923"
                     /pseudo
     CDS             12046..13353
                     /gene="puuP"
                     /locus_tag="KLH32_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
     gene            13468..13713
                     /locus_tag="KLH32_000924"
     CDS             13468..13713
                     /locus_tag="KLH32_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBE0137863.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14026..15669
                     /gene="sapA"
                     /locus_tag="KLH32_000925"
     CDS             14026..15669
                     /gene="sapA"
                     /locus_tag="KLH32_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBE0137864.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15666..16631
                     /gene="sapB"
                     /locus_tag="KLH32_000926"
     CDS             15666..16631
                     /gene="sapB"
                     /locus_tag="KLH32_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBE0137865.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16618..17508
                     /gene="sapC"
                     /locus_tag="KLH32_000927"
     CDS             16618..17508
                     /gene="sapC"
                     /locus_tag="KLH32_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBE0137866.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17508..18500
                     /gene="sapD"
                     /locus_tag="KLH32_000928"
     CDS             17508..18500
                     /gene="sapD"
                     /locus_tag="KLH32_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBE0137867.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18502..19308
                     /gene="sapF"
                     /locus_tag="KLH32_000929"
     CDS             18502..19308
                     /gene="sapF"
                     /locus_tag="KLH32_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBE0137868.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19376..19729
                     /locus_tag="KLH32_000930"
     CDS             19376..19729
                     /locus_tag="KLH32_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBE0137869.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20097..20885
                     /gene="fabI"
                     /locus_tag="KLH32_000931"
     CDS             20097..20885
                     /gene="fabI"
                     /locus_tag="KLH32_000931"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBE0137870.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21029..22156
                     /locus_tag="KLH32_000932"
     CDS             21029..22156
                     /locus_tag="KLH32_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBE0137871.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22224..24158
                     /gene="rnb"
                     /locus_tag="KLH32_000933"
     CDS             22224..24158
                     /gene="rnb"
                     /locus_tag="KLH32_000933"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBE0137872.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24236..24338)
                     /locus_tag="KLH32_000934"
                     /pseudo
     CDS             complement(24236..24338)
                     /locus_tag="KLH32_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24392..26366
                     /gene="pdeR"
                     /locus_tag="KLH32_000935"
                     /pseudo
     CDS             24392..26366
                     /gene="pdeR"
                     /locus_tag="KLH32_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26514..26687
                     /locus_tag="KLH32_000936"
     CDS             26514..26687
                     /locus_tag="KLH32_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137873.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26777..27526
                     /locus_tag="KLH32_000937"
     CDS             26777..27526
                     /locus_tag="KLH32_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0137874.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27795..28013
                     /gene="osmB"
                     /locus_tag="KLH32_000938"
     CDS             27795..28013
                     /gene="osmB"
                     /locus_tag="KLH32_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBE0137875.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28139..28465)
                     /gene="yciH"
                     /locus_tag="KLH32_000939"
     CDS             complement(28139..28465)
                     /gene="yciH"
                     /locus_tag="KLH32_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBE0137876.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28465..29202)
                     /gene="pyrF"
                     /locus_tag="KLH32_000940"
     CDS             complement(28465..29202)
                     /gene="pyrF"
                     /locus_tag="KLH32_000940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBE0137877.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29395..30564)
                     /gene="lapB"
                     /locus_tag="KLH32_000941"
     CDS             complement(29395..30564)
                     /gene="lapB"
                     /locus_tag="KLH32_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBE0137878.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30571..30879)
                     /gene="lapA"
                     /locus_tag="KLH32_000942"
     CDS             complement(30571..30879)
                     /gene="lapA"
                     /locus_tag="KLH32_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBE0137879.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31028..31792)
                     /gene="pgpB"
                     /locus_tag="KLH32_000943"
     CDS             complement(31028..31792)
                     /gene="pgpB"
                     /locus_tag="KLH32_000943"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBE0137880.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31962..32552
                     /gene="ribA"
                     /locus_tag="KLH32_000944"
     CDS             31962..32552
                     /gene="ribA"
                     /locus_tag="KLH32_000944"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBE0137881.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32616..35291)
                     /gene="acnA"
                     /locus_tag="KLH32_000945"
     CDS             complement(32616..35291)
                     /gene="acnA"
                     /locus_tag="KLH32_000945"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBE0137882.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35455..35550)
                     /locus_tag="KLH32_000946"
     CDS             complement(35455..35550)
                     /locus_tag="KLH32_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137883.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35664..35831)
                     /locus_tag="KLH32_000947"
     CDS             complement(35664..35831)
                     /locus_tag="KLH32_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137884.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35834..35998)
                     /locus_tag="KLH32_000948"
     CDS             complement(35834..35998)
                     /locus_tag="KLH32_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBE0137885.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36293..37267)
                     /gene="cysB"
                     /locus_tag="KLH32_000949"
     CDS             complement(36293..37267)
                     /gene="cysB"
                     /locus_tag="KLH32_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBE0137886.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37477..40074)
                     /gene="topA"
                     /locus_tag="KLH32_000950"
     CDS             complement(37477..40074)
                     /gene="topA"
                     /locus_tag="KLH32_000950"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBE0137887.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40454..40705
                     /locus_tag="KLH32_000951"
     CDS             40454..40705
                     /locus_tag="KLH32_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBE0137888.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40741..41790)
                     /gene="sohB"
                     /locus_tag="KLH32_000952"
     CDS             complement(40741..41790)
                     /gene="sohB"
                     /locus_tag="KLH32_000952"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBE0137889.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42010..42768
                     /locus_tag="KLH32_000953"
     CDS             42010..42768
                     /locus_tag="KLH32_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBE0137890.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42765..43355
                     /gene="cobO"
                     /locus_tag="KLH32_000954"
     CDS             42765..43355
                     /gene="cobO"
                     /locus_tag="KLH32_000954"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBE0137891.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43395..44267)
                     /gene="rluB"
                     /locus_tag="KLH32_000955"
     CDS             complement(43395..44267)
                     /gene="rluB"
                     /locus_tag="KLH32_000955"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBE0137892.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44368..44988)
                     /locus_tag="KLH32_000956"
     CDS             complement(44368..44988)
                     /locus_tag="KLH32_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBE0137893.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(44985..45866)
                     /gene="yciV"
                     /locus_tag="KLH32_000957"
     CDS             complement(44985..45866)
                     /gene="yciV"
                     /locus_tag="KLH32_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBE0137894.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46004..46048
                     /gene="trpL"
                     /locus_tag="KLH32_000958"
     CDS             46004..46048
                     /gene="trpL"
                     /locus_tag="KLH32_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBE0137895.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46025..46118
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46140..47702
                     /gene="trpE"
                     /locus_tag="KLH32_000959"
     CDS             46140..47702
                     /gene="trpE"
                     /locus_tag="KLH32_000959"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBE0137896.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47702..49297
                     /gene="trpD"
                     /locus_tag="KLH32_000960"
     CDS             47702..49297
                     /gene="trpD"
                     /locus_tag="KLH32_000960"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBE0137897.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49298..50659
                     /gene="trpCF"
                     /locus_tag="KLH32_000961"
     CDS             49298..50659
                     /gene="trpCF"
                     /locus_tag="KLH32_000961"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBE0137898.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50671..51864
                     /gene="trpB"
                     /locus_tag="KLH32_000962"
     CDS             50671..51864
                     /gene="trpB"
                     /locus_tag="KLH32_000962"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBE0137899.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51864..52670
                     /gene="trpA"
                     /locus_tag="KLH32_000963"
     CDS             51864..52670
                     /gene="trpA"
                     /locus_tag="KLH32_000963"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBE0137900.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53051..53230
                     /locus_tag="KLH32_000964"
     CDS             53051..53230
                     /locus_tag="KLH32_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBE0137901.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53316..53816
                     /locus_tag="KLH32_000965"
     CDS             53316..53816
                     /locus_tag="KLH32_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBE0137902.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53862..54368
                     /locus_tag="KLH32_000966"
     CDS             53862..54368
                     /locus_tag="KLH32_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBE0137903.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54428..55066)
                     /gene="ompW"
                     /locus_tag="KLH32_000967"
     CDS             complement(54428..55066)
                     /gene="ompW"
                     /locus_tag="KLH32_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBE0137904.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55306..55395
                     /locus_tag="KLH32_000968"
                     /pseudo
     CDS             55306..55395
                     /locus_tag="KLH32_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55423..56166
                     /locus_tag="KLH32_000969"
     CDS             55423..56166
                     /locus_tag="KLH32_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBE0137905.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56196..56735
                     /locus_tag="KLH32_000970"
     CDS             56196..56735
                     /locus_tag="KLH32_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBE0137906.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56840..57238
                     /gene="yciA"
                     /locus_tag="KLH32_000971"
     CDS             56840..57238
                     /gene="yciA"
                     /locus_tag="KLH32_000971"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBE0137907.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57278..58006)
                     /gene="tonB"
                     /locus_tag="KLH32_000972"
     CDS             complement(57278..58006)
                     /gene="tonB"
                     /locus_tag="KLH32_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBE0137908.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58221..58517
                     /locus_tag="KLH32_000973"
     CDS             58221..58517
                     /locus_tag="KLH32_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBE0137909.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14051 a        15133 c        14624 g        14712 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacctactaa tgcaccttcc tgctgtaatg cctcgaccag
       61 cgctaatatt tcgctttctg gaagcaagcc acgcagtggg ttcaacccga tgccatcgtg
      121 agaggcgagg aagttaaacc aggtcgtttt actggagggc aagctcagac tttgcgccca
      181 ctggcacagc gcctcgacgt tctgtttttg caccgcatgt aacaccagcg gcggcagcga
      241 gaactggtac accatatgcg cttcgtcatc accgttgcca aagtaagcaa tgttgtcttt
      301 gtgcggaaca ttggtttcgg taatgatcac tgtacctggt gctacgtcat caataatcga
      361 ccgtaacagc ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
      421 tttccacata aagccaacgg catccaggcg gacatactca gcacctttct caaggtaaca
      481 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
      541 atcgtcacta aaggtggtcc ataaatggcg cgtagaatta tcatccattt gaaacggcgt
      601 taatagcggt aacgcacgcg gaagggtgac ggcactgaga tcggttttcg gatcaaccgc
      661 aataaagaaa tcttcaaaac ccggaagctg ttgtaaatag tttttaaacc attcactttt
      721 tgcagacata tggttgcaga catatggttg cagacaaaat caaacattaa atgactgcat
      781 tcaccgagtt gctgaatatc ctgccactcc cccgcttcac tggcgacctg atgataatca
      841 attaccgaaa atccatcatc agatgaccat gggtaaaaag gtaataaatg aacatgtgaa
      901 aaaatgcttt gcagccattg atggtagaac tgattaaata tgggtagtgg ttttaaatca
      961 ttactgtgaa attgatcggc ataggtaatt aacacgacat cactttcatc ccagtgtttt
     1021 ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac gcgttacaag tttctgtaaa
     1081 tgagttgcgg taaatgttcc accgtagatt tcgtccatgt aatccgtaat tttctgtttc
     1141 atgttgcctt tccgtggtag cggtcccatg aggcgagaga ctactccgca gaaaataaac
     1201 tgtcaattga gcacaagggt tttagtcacg caggaaatca ttttttacca gggaaaaagc
     1261 gtatttgtga gctgacgcaa ataatctata gggatgtaaa taaggtgtga cagaaaaaaa
     1321 acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg gcattgcgat
     1381 gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa ggagctcttt
     1441 tgcctgcccg gactggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg
     1501 aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg ccccctgaac
     1561 gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag cggcgaaaac
     1621 gggcagtgaa aacaccagtg ccagagcaag taagcctttt tttaaacatg taagcgtcct
     1681 tctatggatg aaaattgcca tccattatac gcctgagatt taacaatttt tgcgaaactc
     1741 ccctcacctt ttataacgct gtgccagttt attagcagcc cgacttaaca gcggttccag
     1801 cgccacagcc agcaacattt tcagcggtcg gcgagcaact gattttatcg cccagcccgc
     1861 cacacccgcc gggccatagc gcagtgcggt aagaagtacc agcttgcctg ctaatttgaa
     1921 accaagcttc accttttgcc cggcctgttg ccagcgagta ttcataactt tcctcacagt
     1981 tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga cataacgttc catctcgcgt
     2041 aaacgcgttt cacttgccgc cagttcacgg tcgacttcat ccagcaattc gctgctggaa
     2101 ggtagctgct caccaaaggc catgttgtcc ggcattggat caagcgcaaa tgacaaaatg
     2161 atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa tcgacagcac caccaggata
     2221 cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc cggcgcagac gccgcggacc
     2281 atgccctgct gtggaatacg ccataacttt ttattgagat taatgcccgc cattagcgat
     2341 ccctccagtt cggatgttct gcatcaagaa tagattccag cgcctgaata cgttcgcgca
     2401 tccgttttgc ttcatcaacc agttgcgcta atcgctgctg ctcactttgc gacaattcac
     2461 tgcgaccaga acgattgctg taatgcagcc ataaccagat cggtaaaaca aacagcacaa
     2521 aaatggttaa cggaatagcc agaaatagcg cgctcataag tactccttag acgatgagcg
     2581 gcggcgcgtt cggacgccgc ctggatgtta ttattgattg tcttgcttca ttttggcttt
     2641 taattgtgcc agttgttcgc tgattgcatc atcggctttc agttcggcaa actgatcgtc
     2701 cagcgatttt tgtttaccga agctgtggct ttctgcttcc gcttccatct ggtcaatacg
     2761 acgttcgaaa gattcgaaac gagccattgc ttcatccagt ttgccactgt ccagctgacg
     2821 acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac atcaatgcct gctggcgagc
     2881 gcgtgtttct ctcaatttgt tttccagctc gccaatctct ttcttcatgc gtgccagcgt
     2941 atcgtccacc agcgtcactt catgttccag ggacttaatc agatcggtca gtttctgttt
     3001 ttcaattaac gctgcacgtg ccagatcctc tctctctttc agcagcgcca gttcggcttt
     3061 ttcctgccat tcaacttcac gcgccgacgc ttgttcaata cggcgagtca gctgtttctt
     3121 ttctgccaac gcacgcgccg aagtagaacg tacttcaacc agtgtatctt ccatctcctg
     3181 gatcatcaga cgcaccagtt tctgtggatc ttccgctttc tctaacagag cgttgatgtt
     3241 ggcattcacg atgtcggcaa agcgagaaaa aatacccata atgttgtcct cttgatttct
     3301 gcgttcagga ttgtcctgct gaactgttaa tacaatttgc gtgccaattt tttatctttt
     3361 tgatttataa agatctgatt gaagaatcaa cagcaacatg ccaggatgaa ttagcgaatt
     3421 acactaacaa gtggcgaatt tcatcatggc cgaatacaaa gataatttac ttggtgaggc
     3481 gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc gcaccgctgg acaaaccggt
     3541 gctcatcatc ggcgaacgcg gcaccggtaa agagttgatt gccagccgcc tgcattatct
     3601 ctcctcccgt tggcaagggc cgtttatttc ccttaactgc gcggcgttaa atgaaaatct
     3661 gctggattcc gaactgtttg gtcacgaagc gggggcgttt accggtgcgc aaaaacgtca
     3721 tcctgggaga tttgaacgtg ccgacggcgg tacgctattt cttgatgaac tcgctacggc
     3781 accgatgatg gtgcaggaaa aattattgcg tgtgattgag tacggtgaac tggagcgcgt
     3841 tggcggtagc cagccgttgc aggtgaatgt gcggttggta tgcgcgacga atgccgatct
     3901 cccggcgatg gtcaatgaag gtacttttcg cgctgacctg ctcgaccggc tggctttcga
     3961 tgtagtgcaa ctgccgcccc tgcgcgagcg cgaaagcgac attatgctga tggcagaaca
     4021 ctttgccatt cagatgtgtc gggaaatcaa gctgcctctg ttcccggggt ttacggagcg
     4081 cgccagagaa acattgctga attatcgttg gccgggaaat attcgtgaat tgaaaaacgt
     4141 ggtggaacgt tcagtgtatc gccacggcac cagcgattat ccgcttgatg acatcattat
     4201 tgatcccttt aaacggcgtc cacctgaaga agccatcgcc gtttcagaaa atacctcgct
     4261 tccgacgctg ccgctggatt tacgcgagtt tcagatgcag caggaaaaag agttgctgca
     4321 actcagtttg caacagggga aatataacca gaagcgcgcg gctgaattac tggggttaac
     4381 ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt tagcgaagtt gagcaataca
     4441 acttgccgga tgcggcgtga atgctttatt cggcatacat aatcaatacg tcgtttgcag
     4501 gcctgataag cgcagcgcat cagacattat tgcgttgggc tattaatcgc tcagcgcatc
     4561 ctgcaaaatt ttcattgccg catcgaattg cgcatccggg atggtcagcg gatacaggaa
     4621 gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc agcccctgcg ccagcgcgcg
     4681 ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc gtttgcggat cgttaaactc
     4741 tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg gccggaacgc tttctttggc
     4801 atcaatcaac gtgtttttga gacgctggcc cagttgattc gcgcgttcgc agagtgattc
     4861 tttgtcgata atattgagca ccgcgtgcgc ggcagccacc gccagcgggt tcccggcgta
     4921 ggtgccaccc aacccgcccg gcgcgggcgc gtccataata ttcgcgttac cgaccacgcc
     4981 cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc attaagtccg gcttatcggc
     5041 gtaatgatcc atggcaaaca gtttaccggt acgcgcaaag ccgctttgca cttcatcggc
     5101 aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg gcggcaacca gctcttttgg
     5161 cgcaacgttg aaaccgcact cgccctgcac cggttcgaaa ataatggccg cgacctgctt
     5221 cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg agggagtcct gtgttgaaat
     5281 gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa ccagggaacg ggccgaagcc
     5341 gattttgtac ggcgcaactt ttccggtcag cgccatggtc atatatgtac gaccgtggaa
     5401 gccaccgcta aacgcaatca cgccaggtcg tccggtatgg gcgcgagcaa ttttcaccgc
     5461 gttttccacc gcttccgcgc cggtagtgaa gaatgcggtt ttagcctgcc cgctcactgg
     5521 ggcaagggtg ttaattttct ccgccagcgt gacgtagctt tcgtacggca caatctgata
     5581 cgccgtgtgg gtaaactgtt gcaattgctg ctccaccgcc gcgaccagat cagggtggcg
     5641 atgcccggta ttcagcaccg caatgcctgc ggcgaaatcg atgtattcgt tgccctctac
     5701 gtccttcagc gtagcgtttt ctgccgactg ggcgaagaag ttacacatca cgccaacccc
     5761 gcgtggggta gcagaaagac gacgctgatg gaattcattg ttgctcatgt ttcacacctt
     5821 ttgactcttc actgttaatc ggaaattaaa cgcccagacg atcgcgcagg ctgtaataag
     5881 ccgcgcccat cgcggtaaat ggcacgcgca gcgtgcgccc gccggggaac gggtaatgcg
     5941 gcagattggc gaaggcatcg aaacgctcgg cgtcaccgcg cagcagttca gcaatcaaac
     6001 gcccggcaag atgggtacag gtgacgccgt ggccgctgta accctgcatg taatagatgt
     6061 tggtatcaag gcggccgaac tgcggcatgt gcgacaaggt cagcaggaag ttgcccgtcc
     6121 agcggtaatc aattttcacg cccttcaact gagggaaggt ttttagtagt ttcggtacca
     6181 caagacgctc aacgtcatcc gggttgcgtg caccatacac tacacccccg ccgtacagca
     6241 ggcggttgtc ggcggtaaga cggtagtaat caagcagata gttacagtct tccacacagt
     6301 agtttttcgg gatcagcgaa cgggctaaat cttccgacag ccgttcggtg gtgatcacct
     6361 gcgtgccgca cggcatgctg cgtttcgcta gttccggctc tactttatcg cccagatacg
     6421 cattcccggc gacaatcaca tacttcgccg tcacctgacc attggcagtc cgaaccacgg
     6481 ctggcgtggt gtgctggatc tgcgtcacgg cggaaagctc atacacgcgc ccgccgttca
     6541 ggcggatggc atcggcttca ccaatggcga ggtttagcgg gtgaatatgc ccaccgctgt
     6601 gatccagcag cgcaccagtg tagcgatcgc tggcgacctc gcggcgaata gcgttgacgt
     6661 ccagcagctc cagctgttta ttgccgtagc gttcccagtt ctctttctgc tcttcgagtg
     6721 tggcgagttg tttatcattc atcgccacaa acagaccgcc ggggcggtag tcgcagtcaa
     6781 tctgataacg tttgatacgt tcgcggatga tctcgccacc ctcgaacatc atgctgccga
     6841 gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac gtcgatgtcg cggctgtagg
     6901 agttcacaag ctgcccgcca ttgcgcccgc ttgcgccaaa gccgatgcgt gaggcttcga
     6961 gaaccactac gtcaaagccc gcttccgcca gatgcagcgc ggaggagagc ccggtatagc
     7021 cgccgccaac cacgcaaacg tcgcaggtga ttgactcatt cagtgtgtcg aatggtgcat
     7081 atttattcgc actggcggcg tagtaactgc tggtatgttc ggtcatttca ggcctccagg
     7141 cttatccaga tggttttcag ttcagtgaat ttttcaaggg catgcaggga tttgtcgcga
     7201 ccgttgccgc tctgcttata gccgccaaac ggcacggtca tatcgccgtc gttgtagtta
     7261 ttgacgaaga cggaaccggc tttcaggcgt cggctcatgc ggtgcgcgcg ggagaggtcg
     7321 cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt tggcaagctg cagcgcctga
     7381 tcttctgatg tgaaacgggt gaccaccagc accggaccga aaatctcttc gcggcttaag
     7441 gacgcattcg ggtccacatc cacgaagatg gtcgggccga tggccgctgc cagcccggcg
     7501 ttgcgaccat ccagcagcag ttgccccttg ccttcgcctt cacgaataaa ggtgtgtacc
     7561 gagtcagcgt gagcgctgtc gattaaggtg cccatggtgg ttgcgggatc aagtggatgg
     7621 cccggctgcc agttttgcgc ctgctgtttt aacagggcta agaattcatc ggcgatgctc
     7681 tcttccagta acaaacgcgt tccggcgatg cacacctggc cctggttgta gaagatgcct
     7741 gcggcggtgg cgcttgccgc tttttgcaga tccggacaat cggcgaaaac gatgttggcg
     7801 cttttgccgc ccgcttccag ccagacacgt ttcatgttgc tatcacccgc gtctttcagc
     7861 agttgtttac ccgtacgcgt ggagccggta aaggcgatgg catcgatatc gttatgacgc
     7921 gaaagcgcct gccccgcttc atgaccaaaa cccgtcacca cgttcaacac accatccggc
     7981 aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac tgagcggtga tttttcagac
     8041 ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc cgagtttcca gcaagtcagc
     8101 aacagcggga agttccacgg cacgatggcg gcaatcacgc cgaccggttc acgcacgatc
     8161 atcgccagct catggctact ggtggtcgcc acttcgccat acactttgtc gatcgcttcg
     8221 gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat cacgcagact gtgacgaatc
     8281 ggtttgccgg tgtcgagagt ttccagcagt gccagctctt cggcgtgggc ttccattaaa
     8341 tcggcgagtt tattcagtac cgctttacgt ttagccggag aagagagtga ccagtcgccg
     8401 cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga tatcgacgct cttgccgcgg
     8461 gcaattttcg ccagcggtgc ctgggtgacc ggatcaacgg tttcaaaggt ttcattttcc
     8521 gccgcagcag tatattcacc gttaataaat aagcggtttt caatggcgag acttaacgct
     8581 ttatcctgcc agtaagccag atgatgaaaa ttcattatga ctcctgtttc acgtctatca
     8641 gatatatgca gtttgtgaac gtcagggcgc agagatcccc gggctgcatg cccgtttatt
     8701 tctggcgaca ttctttatgg attgccgggc gcattgcgct tacccggctt acagaacaaa
     8761 aagattaaaa cgtggtgggc gtatgggcgc tgataattcg gcaaatacct gccgacgtat
     8821 tactgaaact gtgcgggatg ccggtattaa tggcataact ttgccccgcg acgaggtggt
     8881 aatcctgacc attaatcgtc agaacaattt caccttccag tactgtgcct atttcctcac
     8941 cctgatgctt aattctttcc ccagtggttg tgcccggctg gtacgtttca aagatcatcg
     9001 ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat tgacacaccc tgactgccca
     9061 tctcaattaa gtcgtcctga ttaatgacga cctgcggctc atcaggtttt tccggctcgg
     9121 aaaagaattc cgagagtgac aaaccataca ccttcagcag cttttgcagc gtactgatgg
     9181 cagggctgac tttatcttgt tctatcgtac tgatagcact gtgagtcagc ccggagagtt
     9241 cggcggcacg acgttgtgaa agcccctgct gctggcggat ttccgacaag cgttttcctg
     9301 gcgccagtcc ctcatcactc atatttgcat ttccttaaac tgtagtggtc agagtcgctg
     9361 tttttcagcg atatggtgct gccaagcggt gataaagccc tcgaacaata tacgcgaaag
     9421 cgcgtactcg ctactgttcc attccgggtg ccactgtacg cccagcgcaa aaggatgatt
     9481 gatgacgcta accgcctcaa ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg
     9541 gctaacgacc ttcgcccctt gtccatgtag agagtttacc caaaagttgc tacattcagg
     9601 taacaacgca gacagtaatc ccccctcttc aacctgaact tcatgcgacg gtgcatattg
     9661 ctgttccacc ggcagttcgg gatcttcccg atgttccagc aattcaggct gttcgcacag
     9721 cttgcgatgc aacgaaccac cggttgccac caccagttct tgtaaacccc ggcagatggc
     9781 gaaaatgggg atgcgccttt cgagtgcggc attaattatc gccatgctca gaagatcacg
     9841 cccgggatcg gcgtcaggct catcgccgtt ttcaccatat aggtgcggct gcacattgct
     9901 gggactacta ggaagataaa tgccatcgag tttcggcaaa agttgttcaa gtaacgacgg
     9961 ttccgccagc gcatgtggta gcgcaattgg caagccgcct gcgtggatga tggcattcag
    10021 gtacttttct tgcagagtct gggtcgcatg acccttaagc ctgttcctgc acattacgac
    10081 accgataacc ggattgttca ttatattttc catgctcaat ctcacaaagt ggactaaatt
    10141 atcgccatta ctgcgcttta aatgaccttt atgttcaata ttttttcaat ctagcagtgg
    10201 attttcattt ttgcaaactc aatttaacat ttgacaaaca tttggtttgc atacagattc
    10261 gaatgatggt cattatattt tacgctttga taacgagcgg aaaacaaacc aaaggcgaag
    10321 aatcatggaa accaatatcg ttgaagtaga gaactttgtt cagcagtcag aagagaggcg
    10381 gggtagcacc tttacgcagg aagtgaaacg ctacctggag cgctacccga atacgcaata
    10441 tgttgatgtt ttacttactg atttgaatgg ttgcttccgt ggaaaacgta tccctgtttc
    10501 aagcctgaag aagctcgaga aagggtgtta tttcccggcc tcggtatttg caatggatat
    10561 tctgggcaac gtagtagaag aagcaggtct gggtcaggaa atgggcgagc cggatcgtac
    10621 ctgtgttcct gttctcggtt ccttaactcc ttctgccgcc gatccagagt ttatcggtca
    10681 gatgctcctg accatggtcg atgaagatgg cgctcccttt gacgttgagc cgcggaacgt
    10741 tcttaaccgc ctgtggcagc agctgcgcca gcgcggattg ttcccggttg tagcggtaga
    10801 gctggagttc tatttactgg atcgccagcg cgacgctgaa ggggatctgc aaccgccctg
    10861 cgcgcctggc accgatgacc gcaatacaca aagccaggtt tactccgttg ataacctcaa
    10921 ccacttcgcc gacgtgctca atgatattga tgaactggcg cagttgcagc tgattccggc
    10981 agatggcgcg gtcgctgagg cctcgccggg tcagtttgaa atcaaccttt accatactga
    11041 taacgtgctg gaagcctgcg atgatgcgct ggcactaaaa cgcctagtgc gtctgatggc
    11101 agaaaagcat aagatgcacg ccacttttat ggcgaagccg tatgaagagc acgcgggcag
    11161 cgggatgcat atccatatca gtatgcaaaa taatcgtggc gagaatgtgc tttctgacac
    11221 agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc
    11281 gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc agccgggaat
    11341 gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc accgtcgccc tgcgtattcc
    11401 gtgcggtgac cgtcataatc accgcgtgga atatcacgtg gcgggtgccg atgccaaccc
    11461 atatctggtg atggcagcga tttttgccgg tattttgcat ggccttgata acgagctgcc
    11521 gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa ggcttaccct tcccgattcg
    11581 ccagagcgat gccctgggtg agtttatcga gaatgatcac ctgcgccgct atttaggcga
    11641 acggttttgc catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct
    11701 catcacagaa acagaaattg agtggatgtt gaaaaacgcg taattcccct tgcggggttg
    11761 tttcatcggc ctgagcggag ccaggcctga catttgctgg taatccagac atttcccgga
    11821 tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta acgattaaga ctgtgacgag
    11881 gaaatgagat gatgccgatg attaaatccc cgcatggcga gggaggctgc gtatgcgccc
    11941 ctcctgctac tgactggact ccgccccctt tgcttccctt gctaaacagg tttgatttca
    12001 ggtcgacacg accgcaaacc ttattacgca ggggaggcag caattatggc tattaattca
    12061 ccactgaata ttgctgcgca acccggcaaa acccgtctgc gaaaatcact gaaattgtgg
    12121 caggtggtaa tgatgggtct ggcctatctc acgccgatga ccgtatttga tacttttggt
    12181 attgtctccg gcattagcga cggtcacgtt ccggcgtcct atttgctggc gctggcgggc
    12241 gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc agtttccgga ggccggttcg
    12301 gcctatacct acgcgcaaaa gtcgattaac ccgcacgtcg gatttatggt cggctggtca
    12361 tcactgctgg attatctctt tttgccaatg atcaacgttt tgttggcgaa aatctatctc
    12421 tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta
    12481 accgccgcga atctgaagag cgtcaacctg gtcgctaact tcaataccct gtttgtactg
    12541 gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg ttcagggact gcataaagga
    12601 gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc gcacctgatc
    12661 ccgattatta ccggtgcgac gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc
    12721 acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc cgaaagccat cttcctgacg
    12781 gcggtatacg gcggcgttat ctttatcgcg gcgtcgttct ttatgcagct gttctttccc
    12841 gatatcagcc gctttaaaga cccggacgcc gcactgcctg aaattgcgct gtacgtcggc
    12901 ggcaagctct ttcagtcgat tttcctctgc accacgtttg tgaacacatt agcgtctggc
    12961 ctggcttcac atgccagcgt gtcgcgtctg ctgtatgtga tggggcgtga caatgtgttt
    13021 ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga ctccggcact caacgtcatt
    13081 atggtcggga ttgtcgcgct gtcggcgctg ttctttgatt tagtcaccgc gacagcactg
    13141 attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc
    13201 tggcggcgta aaggaatgaa taaaagctgg aaggatcact tccactattt gctgatgccg
    13261 ctggttggcg cgctgacggt gggtgtgctg tgggttaacc tagagtcaac gtcactgaca
    13321 ctcggtctgg tatgggcttc gttaggcggc gcattatcct gcctgagggt aacgcgccgc
    13381 gccattaact ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc
    13441 ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat
    13501 attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg
    13561 ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagcgaa
    13621 gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc
    13681 gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt
    13741 atcgttgtcc ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg
    13801 tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt
    13861 tatagttata gagaaatcac gggtacgaat gttgaacaga tggcgacgcc acctgttcaa
    13921 cgaccaacca cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat
    13981 taaatattag taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta
    14041 tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc
    14101 cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac
    14161 acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt
    14221 tatgatcgac tgctggatgt cgatccctat acctatcgcc tgatgcctga acttgccgaa
    14281 agctgggaag tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg
    14341 tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt
    14401 accttccagc gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc
    14461 ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat
    14521 cataccgttg agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc
    14581 cattatgctt cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag
    14641 gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc
    14701 gggcaattta ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg
    14761 caggtagtgg tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg
    14821 gaatgcgacg ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg
    14881 cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc
    14941 aaaccgccgc taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag
    15001 cgcctgatgc aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc
    15061 gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc
    15121 gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt
    15181 tcgcaggcgt ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg
    15241 caggttggcg taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg
    15301 atggatatga gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac
    15361 agtttcttcc gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac
    15421 tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca
    15481 gcgcgtattg aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg
    15541 ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt
    15601 agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa
    15661 aaaccatgat tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc
    15721 tgaccttcgt tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt
    15781 cactgtggaa tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt
    15841 ccagcattaa cggtcaacca attgccgaac agttaaaaga gctcttcccg gcgacgatag
    15901 aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg
    15961 ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct
    16021 tttcaatccc ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag
    16081 gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt
    16141 ttgcgttgat tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg
    16201 caatccgcca tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga
    16261 tccgcctgat gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg
    16321 cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac
    16381 cgccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca
    16441 ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc
    16501 aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta
    16561 acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg
    16621 ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc
    16681 aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta
    16741 ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat
    16801 caattattgc cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac
    16861 gacctcggac gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc
    16921 gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg
    16981 gcgacacacg gcctccgctc agcggtgctt aaccatattc tggatacctt actggcgatc
    17041 ccttcgctgc tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct
    17101 atgtttgctg tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg
    17161 catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg
    17221 aatattctct ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc
    17281 cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca
    17341 caactcccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc
    17401 gccccgtgga ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac
    17461 ctccttggcg atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg
    17521 atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc
    17581 gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg
    17641 gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg
    17701 ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca
    17761 aactggttgg ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt
    17821 cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt
    17881 ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga
    17941 ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc
    18001 agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac
    18061 cgacaaactc aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca
    18121 accaaaacag caataccact attttgctta tcagccatga cttacaaatg cttagccaat
    18181 gggcggataa aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg
    18241 agttggtgac gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact
    18301 tcggcagcgc gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc
    18361 tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat
    18421 gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc
    18481 tgaacatgga gaaaaagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa
    18541 acgttccgct accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg
    18601 agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa
    18661 tccacactgg caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt
    18721 gacgatcatc cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt
    18781 tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt
    18841 ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg
    18901 atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc
    18961 ggacaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt
    19021 gccgatgaag ccctcgcttc actggatatg tcaatgcgtt cgcagttgat taatctgatg
    19081 ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg
    19141 atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc
    19201 agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg
    19261 cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc
    19321 tccttccaga ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat
    19381 ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa
    19441 gctctggcga tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc
    19501 ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga
    19561 tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc
    19621 gcagggctgg accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt
    19681 gctggagatt attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg
    19741 ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc
    19801 ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt
    19861 ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt
    19921 tagggtaagg ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta
    19981 tagctactca cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa
    20041 acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg
    20101 gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct
    20161 acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg
    20221 acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc
    20281 agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt
    20341 ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg
    20401 gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct
    20461 acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc
    20521 tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg
    20581 caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg
    20641 tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag
    20701 acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt acagttacta
    20761 ttgaagatgt gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg
    20821 gtgaagtagt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga
    20881 aataatcgtt ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc
    20941 tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa
    21001 cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca
    21061 ctggtatcat gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga
    21121 agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc
    21181 ccccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca
    21241 gcttaccgtg acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac
    21301 ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc
    21361 gctccccgga cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata
    21421 tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa
    21481 tgacgtcggt cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg
    21541 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt
    21601 acccgggctg gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg
    21661 ctggcgaagc agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc
    21721 agaactttgc caactggccg aaacacactc tctccaccct atttcacttt cccttctcga
    21781 aaagttgtta aacagcacac gggtcaatgc acagccggat aatcagcttg cggcgttgtt
    21841 atgcgcgcgt ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga
    21901 atataaaaaa atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga
    21961 ccgtcattct gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga
    22021 tcgctttagc gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat
    22081 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt
    22141 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
    22201 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
    22261 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct
    22321 ttggcttcct ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa
    22381 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
    22441 cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca
    22501 aaaatgaccg tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc
    22561 gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc
    22621 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
    22681 ttggtgacga ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag
    22741 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga
    22801 cctcgctgga ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt
    22861 tcgctaaggc gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg
    22921 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
    22981 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
    23041 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca
    23101 ccactgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
    23161 atgaccaggt ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa
    23221 ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata
    23281 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg
    23341 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
    23401 tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct
    23461 ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa
    23521 cgcacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc
    23581 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
    23641 catttgctga aattagctct gaacccggtc ctcactttgg cctcggtctg gaagcatacg
    23701 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
    23761 cggttatcaa aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg
    23821 aacgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct
    23881 tcctgaaaga taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc
    23941 gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt
    24001 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta
    24061 aaggtgaaac ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca
    24121 tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct
    24181 catggatggg ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac
    24241 tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa
    24301 acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc
    24361 ggactccgct gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt
    24421 gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt
    24481 gcgcttttct accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca
    24541 ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt
    24601 cgatgaaagc gatctttctg tgcatctggt aggacgaaaa atcaataaac gggaatgggc
    24661 tggcaacgcg tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct
    24721 ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg
    24781 ggggaatatc caacgcttca atcggttatg tgaagattac acagggttga aagaacacga
    24841 cgtcattggg taaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag
    24901 gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc
    24961 aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg
    25021 caaaaacgag atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca
    25081 ggagcgactg cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc
    25141 aatgcaggag ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt
    25201 gtatcttgat ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga
    25261 ccagttatta cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt
    25321 ggcgcgtcca ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct
    25381 ggaagcaatg gcatcacgaa ttttgacccg cttacgcctc ccctttcgta ttggtttaat
    25441 tgaagtttat accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag
    25501 cgcgactatt attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg
    25561 acaattttgc gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga
    25621 taccaacttg cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg
    25681 gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc
    25741 caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt
    25801 gggtgattct cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc
    25861 gagtggcggt aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga
    25921 aacaggttct ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga
    25981 gttgtctgat tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag
    26041 gtgcgcaagt gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc
    26101 gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac
    26161 ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg
    26221 tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca
    26281 atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt
    26341 ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg
    26401 tcattatata tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta
    26461 gcggcaattt ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg
    26521 aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag
    26581 gcgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg
    26641 ttgctgaatc tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc
    26701 aggttttcct atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt
    26761 tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca
    26821 gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg
    26881 ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt
    26941 ttcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga
    27001 actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg
    27061 cagcagcaat gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat
    27121 cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct
    27181 cggtggcgtg taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat
    27241 ccagcaggta catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg
    27301 attcaccggt cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg
    27361 cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg
    27421 tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat
    27481 gcatcttgag cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc
    27541 tacggatgat ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta
    27601 agactattta cctgttatta acactctcaa gatataaaat tattatcagc gatataacag
    27661 gaagtaatta tcacctgcgt gatataaccc tgcgcgcgag cagatttcac ggaataattt
    27721 caccaggctt attcttagct attatagtta tagagagctt acttccgtga atcataaatt
    27781 caggagagag tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta
    27841 ctttggcaat gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa
    27901 tcggcgcggg tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta
    27961 cattaggtgg tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa
    28021 taaatgtcga ttatcgactc gcaattataa tacgcttgat tctgttcagt acgaaataac
    28081 tgaataatac acacagtaaa atattcgaaa gccacggata tatccgtggc tttttatgtt
    28141 aaccgcctgc gagttttacc ttcatccctt tcgcttcaag tagtgatttt aataaatcac
    28201 gcttatcgcc ctggatttca ataactccat ctttaactgc tccgccgcag ccgcattttt
    28261 tcttcagttc cgctgcaagt tttgtcagtt cggcatcatc gagatcgata ccggtaatca
    28321 ggcaaacgcc cttaccttta cgtccactgg tctgacgctg aatacgcacc gctccatcgc
    28381 ctttagggcg tactggggcc gctttgggct catcaatacg cccggtttcc gttgagtaga
    28441 ccagacggct gttggaatca ctcatcatgc actccgctgt aaagaggcgt tgatcgcttt
    28501 cagtgtctgc gctggatcta ccgattgcgt taccgggcga ccaatcacca tataatcaac
    28561 accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc tggtcaccag cttcactccc
    28621 ctgcggacga atgcccggtg taaccagttt gaactcctga ccgaatacct gtttaaagcg
    28681 cacagcttcc tgagcagaac acaccacacc atcaaggtca catttttgcg tcagtgctgc
    28741 cagacgttct gcataatctg caggtgacag tgtcacgcca agatcggcca ggtcgctggc
    28801 ttccatgctg gtcaacactg tcacagcaat caaaagcggt gcatctttgc caaacggaac
    28861 cagtgcctca cgcgctgcgg tcatcatacg cgccccaccg gaggcatgaa cattcaccat
    28921 ccacacgcct aagtcagctg cagcagcgac agcgtgcgct gcagtgttgg ggatatcgtg
    28981 gaatttcagg tcaagaaaga tatcaaaacc acgctgttga agttcgcgca caaactgtgg
    29041 cccaaacaat gtaaacatct ctttaccgac cttcagacga caatcgcgtg ggtcgatctt
    29101 gtcgacaaag gacagcgcgt catcacaatt atgataatca agggcaacaa ccacaggaga
    29161 attcgtaacg gcgcgggaag aagatgaagc agttaacgtc atgaccagac cttcttgatg
    29221 atgggcaccg ggagtggcgc gaaacaggta aacggcgagc attctacccg tgaaaataaa
    29281 aaattaacag ttgcgatttt tcccctagca ggctttttct gctccgtcgt tgccggagca
    29341 caatgggaca tcaataacta gtatgttgta actaaagtcg gattttttta aaaattacag
    29401 gccatcaaga ccgcgaatcg gtttaatagt tgaccaggca cgacaagacg gacaatgcca
    29461 gtagagggtg tatgcagtaa aaccacattt ctggcagcta taacgaggct tactacgcac
    29521 cttctcgcca accatgtcac gcagcaccat cagactctct ttggcgcgcc cttcttccgc
    29581 ttcatttaag tggtaatcca ttagcttatg gaatacacgc atggtcggat gacgctgaag
    29641 ctggcgtgta atatagacct gtgcagcctc actaccgtcg cgcgcttcga tgatatcagc
    29701 aagcatcaat tcagcatcgg caccggtgtt ctcttccacc gcgcgttgca ggaattctgc
    29761 ccattcggca gttttaccca actgctgata gcaggtttgc aacatttcca gcgtttcgct
    29821 gaccagttct ctgtcctggg atatgacgcg ttgcaaactt tcgacggctt tggcgtattc
    29881 tcctttcgcc ataaacacgc gtcccatcat gatggatacg cgggcgctgt ttttatctgc
    29941 cgccgcccct ttttttagca aggtcatggc acgatcgaga tcgtcgctgg ccatatgctg
    30001 cagggctaac tcacagtaga aatgggcaat ttcgacgcgc tgtttatctt tacccagctt
    30061 caccaggcgt tcggcaacat caattgcttt ctgccactca ctggtagcct ggtagatttg
    30121 tagcaactgt tgcagcgcgc caatgcggaa gtcagtttca tcggtcagct gattgaacat
    30181 gtcttccgcg cggtcgtata acccggcggc catgtaatca cgccccagtt gttgaatcgc
    30241 caacagacgc tgttcatagg tcagcgaggc gctttccatt agggtctgat ggatgcgaat
    30301 agcgcgatca acttcgccac gcgaacggaa caggtttccg agcgtaaggt gggcttcaac
    30361 ggtgcctgta tcctctttaa gcatatcgag aaacaggtct accgctttat cctgttgatt
    30421 actaagcagg aagttaaccc ccgctacgta atcacgcgac aagcggttgg cttcatcttg
    30481 cttgttttgt tgcgcacttc tgcggcccat ataccagcca taggcagcgg ctacaggcaa
    30541 aagcagaaac aacaactcca gcatagaaag ttattccttc gccgctgacg agtgcggcac
    30601 tacagccacg tcagtcgcgg gtgaaagctg gttttccagt cgctttattt tacgttcagc
    30661 gcgcgccagg gaaacacgaa ctcgcagcca gaacaggcca caaatcaacc aaccgatagc
    30721 aaaccccgca gcaaacaata ccgccagcaa tgtggaaata cggtactccc cttgcgctaa
    30781 cagataatta aacgtcacct gttgatcgtt ctgcgcaccc aacgtgaccg aaatcacgaa
    30841 gatcgctaac accagtaaga aaatgagtaa atatttcaca ttacttcccg ttatgtggtt
    30901 agagcgaata aattatggtc aacttaccgc aatagccctt taaagtacca ttttcgcgcc
    30961 gtctgcgaaa tggcatccac gcgcattgtt gatgatttaa ggcctaaatt cggaaaatca
    31021 accagcgtta actttcttgt tctcgttgtg caatttcgcg attttcttcc gcaggtggtg
    31081 ttaatggccc acaaattcgt tgcgcaagcc acgttgccac cgccaccagc gcccacgaaa
    31141 tcaacgtagc taccaccaga tcgcgtggcc aatgcatccc gagcagcagg cggcttccca
    31201 tgactcccgt tgcccagacc aacaagatag caatggttaa cgttcgccga cgcggccaca
    31261 gcaaaccaac ggccagcagt gcccaactgg cagcaaacat cgtgtgaccg gaaggaaagg
    31321 caaaccccgt ctctttctgc cagtgtgaac gcaaatattg tggaatattt ttctcttcag
    31381 ccaactgttc ttttactaga tttccgcgtt ctgctcgctt taaagtgtag aactcatcaa
    31441 ccggaatatg atgtgttttt tccaaccaga taacaaaagg tcgtggttcc tgtactttgt
    31501 ctttgatcca ggatttaacg ccttgtccaa caaggattgc cgccgccaga atggcaaata
    31561 atacaaaggc agccttaatg cgaaaacgca gacaccagag aaaccagccg aacaaaatca
    31621 aatgtgtaat gacgccccag ggctgggtga cagtttcagt aacccaaaaa gccgctttta
    31681 gtagccaact ttgttctcca ggttgccaac gccagccaga aatccatacg gctactggca
    31741 tgacaagcaa tagtgcagct cccactgcgg tacgtcgggc aatcgaacgc atggcctctc
    31801 ctttttgata agtcccacaa tcataactga aaatgccagt tccaagaaaa attgacagat
    31861 ttgtgccatt ccgtgaacga tcgacgcgtc gtgattaggt gaaccccttc tcgttatggc
    31921 aaaataagcc aatacagaac cagcattatc tggagaattt catgcagctt aaacgtgtgg
    31981 cagaagccaa actgccaacc ccatggggcg atttcctgat ggtgggattt gaagaactgg
    32041 caaccggaca cgatcatgtc gcgctagtct atggcgatat ttccgggcat accccggtac
    32101 ttgcgcgcgt ccattccgaa tgtctgaccg gtgacgccct gttcagcttg cgctgcgatt
    32161 gtggcttcca gctcgaagcg gcattgacgc aaattgccga ggaaggccgt ggtattttgc
    32221 tgtatcaccg tcaggaaggt cgtaacattg gtctgctgaa taaaatccgc gcttacgcac
    32281 tgcaggatca aggttacgat accgtagagg ctaaccacca gttaggcttc gccgctgatg
    32341 agcgcgactt cactctttgc gctgatatgt tcaaactcct tggcgtcaat gaagtccgct
    32401 tgttaaccaa taacccgaaa aaagtcgaaa ttctgaccga agcagggatt aatattgttg
    32461 aacgcgtacc attgattgta ggtcgtaacc ccaataacga acattatctc gataccaaag
    32521 ccgagaaaat gggccatttg ctgaacaaat aaccctcttg catcgtgtaa ttcatttgct
    32581 tgccggaagc aaaataaccg gcaagcaaat acttgttact tcaacatatt acgaatgaca
    32641 taatgcaaaa tgccgtcgtt ctggtagtag gtcaactccg tcgcggtgtc gatacgacaa
    32701 cggcagggta cgacttcctg gctaccatcc gcgcgcgtaa gcgtcaccgg aaccgtcgcg
    32761 ccaggttgta gattttgcag atcgccaata tcaatcttct cttccccggt tagccctaac
    32821 gttttacgcg tcacgccttg cggaaattcc agcggcagga tgcccatgcc aattaaattc
    32881 gaacggtgaa ttcgttcaaa cgattcggca atcaccacac gaattccaag cagacgaggt
    32941 ccttttgccg cccagtcacg gcttgagcct gatccatact ctttcccggc aatcaccgcc
    33001 agcggcgttt gctcctgctt atagcgcatc gcagcatcat aaatagagac tacgtcgctg
    33061 tcaggtaaat gccgcgtcat ccccccttca acgccaggca ccatttcatt acggatgcga
    33121 atattggcga aggtgccgcg catcatcact tcatggttac cacgccgcga accgtaggag
    33181 ttaaagtctt ttcgctcaac accccgacct tgtagatatc gacccgctgg gctgtcgggc
    33241 ttaatactgc ccgccggaga gatatggtca gtggtgactg aatcccccag cattgcgagg
    33301 atccgcgcac cgtgaatatc ttccactggt gctggtgttg cctgcatttc atcaaagaaa
    33361 ggcgataagc gaatataggt tgagtcctcc tgccaaccgt aggtatcgga tcgtgtgacg
    33421 ttaatttcct tccactctgc tgtgccttca aaaacttctg cgtactcttt gcggaacatt
    33481 tctgtggaga cttgttctac cgcacgggca atttcttgtg ccgatggcca gatatctttc
    33541 agataaaccg gctcgccttt gcgatcatgg ccgataggct cagaagccag gttgatattc
    33601 atatttcccg ccagcgcata ggcaaccacc agcggtggcg aggccagcca gttagtttta
    33661 accagcggat ggatacggcc ttcaaagtta cggttgccgg acagcaccgc accgacggtt
    33721 aaatcgcctt ttttgattgc cgtttcgata ggatcgggca gcggcccaga gttaccaata
    33781 caggtggtac aaccgtatcc cacaaggtta aaccccagtt cgtcgagata cggtgtcagt
    33841 ttcgcttttg ccagataatc agaaacgact ttcgaacccg gtgccaacga cgctttgacc
    33901 cacggttgcc gcttgaggcc aagagttacg gcttttttcg ccagcaagcc tgcggccatc
    33961 agcacacttg ggttagaggt gttggtgcac gaggttatcg cagcaatgac cacagcgcca
    34021 tcaggtaact gatactgatg tccgttcata acataatcga ctggcaggcg atctttatgc
    34081 gtggcattca cttccagttc gttactggcg gcaaatgctt ttggtacatc gggcagtgca
    34141 acgcgatcct gtgggcgttt tggccctgcc aggctcgctt caacgtcatt catatccagt
    34201 tctaacatac tggtaaaaat cggttcatcg cccgggttac gccacatgcc ctgcgctttg
    34261 gcatattttt cgaccaactc gacctgatct tcgctgcgcc cgcttaaacg catgtaatcg
    34321 agggttactg catcgattgg gaagaagcca caggtggcac catattctgg cgacatattg
    34381 gcaatggtgg cgcgatccgc caacggtagt gaatccagac catcaccata aaattcgacg
    34441 aatttcccca ccacgccatg tttgcgcagc atttgggtaa cagtgagaac caggtctgtg
    34501 gcggtaatac cttcacgtaa ttttccggta agtttaaagc ccactacgtc cgggataagc
    34561 atggaaaccg gctggcctaa catggcggct tctgcttcga tcccacccac gccccacccc
    34621 agcacgccaa ggccgttgat catagtggtg tgcgagtcag taccaacgag tgtatccgga
    34681 taagcaatcc attcaccgtc ctgcaattcg ctccacactg ctttgccgag atattcgagg
    34741 ttaacctgat ggcaaatgcc tgtgcctggc ggcacgacgc taaaccgact gaacgcttgc
    34801 tttccccatt tcaggaacac ataacgttcg tggttgcgct ccatttccag gcgtacgttt
    34861 tcttcaaatg cctcatcatc accaaaacga tcgacggtca ccgagtggtc aatgaccagg
    34921 tcgaccggtg agagcgggtt aacctttgca gtatcgccac cgaggcgttt aaccgcttcg
    34981 cgcattgccg ccagatcaac aacggcaggt acgccggtaa agtcctgcat cagcaccctt
    35041 gccgggcggt aggcaatttc acggtcagca tgggcatttt tcagccatcc cgccagtgcg
    35101 tggatatcct cttcggtaac tgagttacca tcctgccagc gcagcaggtt ttcgagcaaa
    35161 actttgagtg acttgggtag acgggtgata tcgcccagtg atttagcagc aagcggcagg
    35221 ctgtagtagt ggtaagtttt atctttggcc tgcaacgtgt ccttactggc ttctcgtagg
    35281 gttgacgaca tagctcctcc ttaatgacag ggttgcgaag ccctgattct cttcagggtg
    35341 aatattaaag ataacacaaa catcgcgtaa cgatttgata acaacccaaa ttgataaaag
    35401 aggagaaacc ttgtggcaaa gcaaatgaca accccgccgc agcggggtca aggatcagtg
    35461 agtaatgatc cagaggagtt cggcccagaa gagcatcgac agagaaaacg cgcccatcca
    35521 ggaccaatat ttcatatttg tgttgatcat aggctacacc cttaacggtt attattttgt
    35581 gttgagataa ctcaagctgt tattgctgcg gtgtattaaa agccctttaa ttcattaaaa
    35641 atgggtaata agaatgatga taattattgt ttcggctgat catcttcagc aaacagttct
    35701 ataaaggctt gctgctgcga tgttagttgc cagtcgtctg gaataacgac ggaacattcc
    35761 cgtttcgttt ctggttcatt gtcactatgc tggcataatg gtgacgactc cagttgctcc
    35821 tgaccgacca tagttatccc cacactttcc aaatcagcag tgcgacaacc acccagaaaa
    35881 gtgcagaacc aagaaaaacc gccagccagg ctttacgttt taattcagga tcacggcggg
    35941 gttcctgatt tccagaaggc attgctaacc tcatataatc gccatcactt atcagcaaga
    36001 cgttgaatga ttggtgtaat taatcatacg atgagacaaa tcctaaagaa actttagtca
    36061 aaaatccagc gaatttacga atcgtctcca gtgaataatt caatcttttg acctgaaata
    36121 gatatttgaa aactggaatt tgcgaggcgg gtaattagac actactttac aggtaattta
    36181 ccctataaat atgagggata aaaatattgt gcacggtata gaaataagta acaaaagaga
    36241 atatattccg gcaccttcgc tacataaaag gtgccgaaaa taacgcaaga aattattttt
    36301 ccggcagttt tatatcttta aacatgacct caatttcttc attggagcgc aatgcgacag
    36361 ccgcatcaac gacatcacgc gttaaatgcg gtgcaaaacg ctgaatgaaa tcatacatat
    36421 aactgcgcaa aaaagtacta cggcgaaaac caattttggt tgtactgtgg ctgaagatat
    36481 cgtgagcatc aacgcgcaca aggtcgggat cggcgaccgg atccaccgcc atgctggcaa
    36541 tgacccctac ccccagccct aaccggacgt aagttttaat gacgtcagca tccgttgccg
    36601 tgaaaacgat acgcggcgtt aaccctgcgc ggttaaaggc agtatccagt tctgaacgtc
    36661 cggtaaagcc gaaggtatat gtcaccaacg gatattgcgc cagttcttca atggtaatgg
    36721 cttttttgcc tgccagcggg tgatccggag tgactacaat agcccgattc cagtggtagc
    36781 acggtaacat cactaaatct tcatacagat gcagcgcttc tgtggcgata gcgaaatcgg
    36841 cattgccttt agagacggca tcagcaattt gtgtcggcgg gccctggtgc atatgcaaag
    36901 aaacgcgggg ataacgctca ataaagcctt tgataacgtt tggtaacgcg tagcgtgcct
    36961 gggtatgcgt ggtggcgata tacagcgagc ctttatccgg ccaggtgtgc tcaccggcaa
    37021 ccgattttat ggcatcgact ttcgacagga cttcgcgagc gatacgaatt atttcttgtc
    37081 ctgctggcgt tacctgcgtc aggtgcttgc cgcttcggga aaaaatttga atgcctagct
    37141 cgtcttccag cattctgact tgtttactga tcccgggttg tgatgtgtaa agtccttccg
    37201 ctgttgatga gacattcagg ttatgattga ccacctcaac aatatagcga agttgttgta
    37261 atttcatgtt aaaccatcca cttagacgcc atcagaacat cgttttttcg tcacacctgg
    37321 cgggtgacta atacgattta ataataaaaa aggtgctaac tataaccact atatcattta
    37381 tagccttagt gtataggtgt gaagaaaaaa taatatgcaa taaaaaaggg ccgctttcgc
    37441 gaccctttgt ttataaaaac ctgacagaat taaaggttat ttttttcctt caacccattt
    37501 gccatcaaca taaaatgctg accagccagt cgcctttccg tctttttccg aagagacata
    37561 ttgctgtttg gttttacggc taaagcgaac catggtctta ttaccttccg gatcctgctg
    37621 tggcgcatcg gccagataac gcagtttttc cggcagacgg tcgcggaagc gataaagctc
    37681 ttccaccagt ggcgcacgcg tttcacgcga tttcgggaaa gtgttggcag ccaggaacac
    37741 accggcagca ccgtcacgca gcacgaaata agcatctgat ttttcgcacg gcagctcagg
    37801 taatggcacc ggatcttctt tcggtggtgc cacttcgccg ttacgtaaaa tcttacgtgt
    37861 gtttttacac tcttcgttgg tgcaggccat gtatttaccg aaacgcccca ttttcaggtg
    37921 catttcagag ccacattttt cacactcaac gatcgggccg tcataacctt taatgcggaa
    37981 ttcgccctct tcgatctcgt aaccgtcgca ggttgggtta ttaccacaga catgcaactt
    38041 acgtttcgga tcgatgagat agctgtccat cgccgtgccg catttcgggc aacgacgttt
    38101 tgcgcgcagc gcgttggttt cagcatcttc gccttccagc acgttcagca cttcgttttc
    38161 cggcaccagg ttaatggtgg ttttgcaacg ctctttcggc ggcagcgcat agccagaaca
    38221 gccaaggaat accccggtac tcgctgtgcg aatccccatt ttgcgaccac aagtcgggca
    38281 gtcaatgctg gtcagaacca tctggttcgg gcgcatacca ccctcttccg gatctttttc
    38341 agctttatct aactgctggg tgaaatccga gaagaagtga tccagtacag ctttccactc
    38401 tgcttcgtga tttgccacct ggtcgaggct gttttccatc tgcgcggtaa agtcgtagtt
    38461 cattaactcg cggaaatttt cttcaaggcg atcggtgacg atttcgccca ttttttccgc
    38521 atagaaacga cgattttcta ctcgcacata gccacgatcc tgaatggtcg aaatgatcga
    38581 cgcataggta gacggacgac cgataccacg tttttccagt tctttaacca gcgatgcttc
    38641 actgaaacgg gctggcggct tggtaaagtg ctgggctggt gttagttcaa cgagcgtcag
    38701 agcatcgcct ttatcgactg ccggtaagat acgatcttca tcgcctttac gcagtgcagg
    38761 catcactttt gtccagccat caaagcacaa aatacgaccg cgtgctttca ggcggaaatc
    38821 gcccgcacca acggtcagcg tcgtggagtc atatttcgct ggggtcatct ggcaggcaac
    38881 gaactgacgc cagattaact ggtacagttt ctgcgcatct gcttccatat ccttcagcga
    38941 ttccgccatc acattgacgt cagaaggacg aatcgcttcg tgcgcttcct gtgagttttc
    39001 tttgctggcg tactgattcg gactttccgg cagatatttc ttaccaaaat tatcgctgat
    39061 ataaccgcga accatattta ccgcgtcctg actcaggtta gtggagtcgg tacgcatgta
    39121 agtgatatag cctgcttcat acaaacgctg cgccatcatc atggtttttt tcacgccaaa
    39181 accaagacgg gtgctggcag cttgttgcag cgtagaggta ataaaaggag cgccaggttt
    39241 actggttgtc ggtttgtctt cacgttccag cacgctgtag cgcgcttttt ccagcagact
    39301 taccgcagcc tgagtttgtt ctttgttgac cggacggaac ggtttgtcat tctgatgagt
    39361 cacctgcaac gccaacgctt caccagatgg cgtagtcgtg ctggcatcga cttcccagaa
    39421 ctcttccggt acgaacgctt taatttcacg ctcacgctct accaccaggc ggaccgccac
    39481 cgactgcaca cgaccggcag acagaccacg agcgatcttt ttccacagca gcggcgaaac
    39541 catatacccc accacgcggt ccataaagcg acgcgcctgc tgggcattaa cacgatcaat
    39601 attcagctca cccggtttgt taaatgcctg gcggatcgcg tttttagtaa tttcgttaaa
    39661 caccactcgg ctatagcgcg catcatcacc accaatcact tcccgcaggt gccatgcaat
    39721 ggcttcccct tcgcggtcaa ggtcggttgc gagatagatg tggtcggctt tttcagccag
    39781 ttgtttcagt tcagagacga ccttctcttt accaggcaac acttcatagt gcgcctccca
    39841 attgtgccac gggtcaaccc ccatacggtt gacgagagcg ccacgttcat cctttttagg
    39901 ctttttagcc gtcttggtgg aggtagagtc ggcactcttt ttggcagctg agccactggt
    39961 cggcaaatcg cggatgtgac cgacgctgga tttcaccacg tagtcacaac ccagatactt
    40021 gttgatcgtt ttggcttttg ccggggactc aacgatgaca agagctttac ccatattcac
    40081 cttacctaat ttgattcttc caggaatgcg tcgcacgttg ataaaccttc cactggcaac
    40141 gagttaccga tatggactcg ctctctgcgg atatcaaccc cttgtcgtga ctctgttcac
    40201 gaaaattcag gtgactgagt taacaggtct tggcctacag gcgctatagc acgacggaat
    40261 tttggtcgaa tgtcaagcaa atctattgcc agattgacga aagcgtcaca ctgtacctga
    40321 taaaatccga tacgcaactt tattagcatg caaaatccaa agttgccagt aaggcgctct
    40381 tgccaccagc gtggtatatt tgtctgcaag ccatgcctgg cgtagactaa tgtccttgtt
    40441 taaggagtaa ataatgaaca aagagacaca acccatcgac cgcgaaacgc tgttgaaaga
    40501 ggccaacaaa atcattcgtg agcatgagga tacgctcgca gggattgaag ccaccggcgt
    40561 cacgcagcgt aatggcgtgc tggtatttac cggcgactac tttttagatg aacaaggatt
    40621 gccaacagcc aaaagcaccg cggtgtttaa tatgttcaaa cacctggccc atgtgctttc
    40681 agaaaaatat catctggttg attaaagcaa aacgcgaggt cttagcctcg cgtttgtctt
    40741 ttacatcaat ggcttttgac cccgctgcca ccagcgtagc aacaatcgat cggcgctctc
    40801 tgccgcgctg ccggtgaatc ggtcaatgag tcgtttacgc tgcatatagc gtacattaac
    40861 cacttcacgg ccttccatca ggctaagaat aacttcatca ctggtgttga tttcatcaac
    40921 caggcctttc tctaccgcct gttgtccgta ccagtgttca cccgttgcca cctgttcaat
    40981 atccagagac ggacgcatac gcttcacaaa atctttaaat aactgatgcg tttcgttcag
    41041 ctcttcgcgg aatttctccc gcccttcttc ggtattttca cccagcaagg tcagcgtgcg
    41101 cttatactgc ccggcggtgt gcagttcgat atcaatatct ttgcttttca ggaagcggtt
    41161 aaagttgggc atttgcgcca ccaccccaat ggaacccaca atagcaaacg gtgcggaaac
    41221 aattttgtcc gccacacagg ccatcatgta accgccgctg gcagcgactt tgtctaccgt
    41281 aacagttaaa ggaatgtttt tatcacgcag acgctgcagc tgcgaagccg ccaacccgta
    41341 accatgcacc atgccaccag ggctttccag acggagcaca acctgatcct gcggtttgaa
    41401 tgctgcgagt accgccgtta tctcttcacg tagcgagttc acttcatggg cgtccatgct
    41461 gcctttaaaa tccagcaccc agacgcgagg tttactgtca gttgccacct cgcccagttt
    41521 ggctttcgct tttgctgctt tcgcttcttg cttgtgcttt ttcttctgcg ctttgtgcca
    41581 ctgtttttgc tgatgtgagt ccatcagcgc cgcggccagt tcttctttca tctccttata
    41641 ctgttcgctg agattgttga cccgtaactc gccacgctgg cgtttattac gttgagcaac
    41701 attgacaata atggcggcaa tcgccgcaat cgctagcaca acggtaacga ttttagccaa
    41761 aaacaaacca tattcagaca acaattccac gagtccccac cttggttaaa caacgctttg
    41821 gctcacagtt tacaacaggc atgttaagcc gtctcgcaga gatgttattg cagcgaaagt
    41881 atgacaaagt atccattttg cggtgttatc gccccaggct gattgaaatg cccttctgtt
    41941 tcaggcataa agccccaaag tcataaagta cactggcagc gcgacagaca acgtcgttga
    42001 ggagtagcca tgcattacca gccaaaacaa gatttactca atgatcgcat tatcctggtg
    42061 acgggagcca gcgatgggat tggtcgtgaa gccgcgatga cgtatgcacg ctatggtgcg
    42121 acagtgattc tgttgggccg taatgaagaa aaattacgtc aggtagccag ccacataaac
    42181 gaagaaactg ggcgtcagcc acagtggttt attctcgatt tgctgacctg cacgtccgaa
    42241 aattgccaac aactggcaca gcgcattgtc gttaattatc cgcgtctgga tggtgttttg
    42301 cataatgccg gattgctcgg cgatgtttgc ccaatgagcg aacaaaatcc gcaggtctgg
    42361 caggacgtca tgcaggtcaa cgttaatgcc acctttatgc tcacccaggc actgcttcct
    42421 ttattactca aatcggacgc cggctcactg gtctttactt catcaagcgt tggacgtcag
    42481 ggacgagcca actggggtgc atatgcagcg tcgaaatttg ccaccgaagg gatgatgcag
    42541 gtactggccg atgaatatca gcagcgcctg cgcgtcaact gcattaaccc aggcggtacg
    42601 cgcaccgcaa tgcgcgcaag cgccttcccg accgaagatc cacagaaact taaaacaccc
    42661 gctgatatca tgccgctcta cctctggctg atgggcgatg acagccgccg taaaaccggc
    42721 atgacctttg acgcccaacc gggccgtaaa ccaggaattt cccaatgagt gatgaacgct
    42781 accaacagcg tcagcagcga gtgaaagaaa aagtagatgc tcgtgtggcc caggcccagg
    42841 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
    42901 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
    42961 aaggcacctg gcctaatggc gaacgcaatc tgctggagcc acatggcgtt gagtttcagg
    43021 tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc gccgcctgcc
    43081 gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat atggttttgc
    43141 ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
    43201 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
    43261 atattcttga actggcggat acggtaagtg agctgcgccc catcagacat gcgtttgatg
    43321 ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg gatatgacgt
    43381 ccggcatact ttgattaacc gttattacgc ccgccagaac gacgactgcc gctcacctga
    43441 ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg gtctttttct
    43501 acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag atagttagtc
    43561 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
    43621 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
    43681 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
    43741 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac gccacgactc
    43801 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta ttcacgttca
    43861 acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt gaacagcagc
    43921 agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc acgcagcttt
    43981 ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca caactcacct
    44041 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
    44101 agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc gccgagttta
    44161 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcgg
    44221 gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga gcttcctttt
    44281 ggtgtcgcct tcacaggcgt cgaacaggag aatcaatggc gccgtatagc gccattttaa
    44341 ggccgcgtag tatacagagt tacgcactta taagaaaggc ttcacatcac ctacgccttc
    44401 acgcaccacg accggcgtat catcggtaag atcaataacc gttgttggtt tctgcccgag
    44461 atagccgccg tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg
    44521 atcggattcg gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc
    44581 cagtgcttca agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt
    44641 gcgtttctcc tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata
    44701 gttgcccggc gtgttgtttt tcatcaaacg aaacgcaacg ttatccacga atgaataggt
    44761 cgagagttca gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg
    44821 aatacgacaa atacgctcca tggcgttttt atcttcaatt ttacagccga gcgcatagcc
    44881 ggaatcagtt ggatagacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat
    44941 cagacgttgc tgcgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct
    45001 gtggtgttct gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag
    45061 agtttacgac ccagttcgat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc
    45121 cataaatgat gctgacgcgc aagtgtggcc agctgggtgc gttcattggg cgattgctgg
    45181 cactgcgcga cttccatcgc gtcgccgtgg tgttcggcaa aatgcgcaac caatcttttc
    45241 agccatttag cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa
    45301 tgatgaatga catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt
    45361 ttcccgcgcg ccagatactt tttaaagaca tctgccattg aactggcttt gccgcactcc
    45421 acaaggaaac gcgcaaaatg accgcgcgtc actgcgccgc cctgcgccag tcgttgtgcg
    45481 ccttccagcg ccccaggtat ttgcgctttt tcaagtcttt cggcaatttg ctgcgcccgc
    45541 tgattgcgcc gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca
    45601 atattcagcc cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg
    45661 ggaataagat tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cgcgattgca
    45721 gctgtggtgt catggtcggt gatcgccagg gtgccgacgc gcatctcgac tgcacggtgc
    45781 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
    45841 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
    45901 cgacatcata acgggtctgg caaatattct gaaatgagct gttgacaatt aatcatcgaa
    45961 ccagttaact agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt
    46021 actgaaaggt tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa
    46081 tcagataccc agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa
    46141 tgcaaacaca aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc
    46201 cgactgcgct ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg
    46261 cagatatcga cagcaaagat gatttaaaaa gcctgctgct gatagacagt gcgctgcgca
    46321 ttacagcatt aggtgacacc gtcacaattc aggcactttc cggcaatggc gaagccctgc
    46381 tggcactact ggataacgcc ctgcctgcgg gtgtggaaag tgaacaatca ccaaactgcc
    46441 gtgtgctgcg cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc
    46501 tttcggtttt tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac
    46561 gagaagcaat gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt
    46621 taccgcaact gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc
    46681 tgatggtgat tgaccatcag aaaaaaagca cccgtattca ggccagcctg tttgctccga
    46741 atgaagaaga aaaacaacgt ctcactgctc gcctgaacga actacgtcag caactgaccg
    46801 aagccgcgcc gccgctgcca gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga
    46861 gcgatgaaga gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa
    46921 ttttccaggt ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct
    46981 attacgtgct gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt
    47041 tcaccctatt tggcgcgtcg ccggaaagct cgctcaagta tgatgccacc agccgccaga
    47101 ttgagatcta cccgattgcc ggaacacgcc cacgcggtcg tcgcgccgat ggttcactgg
    47161 acagagatct cgacagccgt attgaactgg aaatgcgtac tgaccataaa gagctgtctg
    47221 aacatctgat gctggttgat ctcgcccgta atgatctggc acgcatttgc acccctggca
    47281 gccgctacat cgccgatctc accaaagttg accgttattc ctatgtgatg cacctcgtct
    47341 ctcgcgtagt cggcgaactg cgtcacgatc ttgacgccct gcacgcttat cgcgcctgta
    47401 tgaatatggg gacgttaagc ggtgcgccga aagtacgcgc tatgcagtta attgccgagg
    47461 cggaaggtcg tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg
    47521 atctcgacac ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc
    47581 aagcgggtgc tggtgtagtc cttgattctg ttccgcagtc ggaagccgac gaaacccgta
    47641 acaaagcccg cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct
    47701 gatggctgac attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca
    47761 gttgcgcagc aatggtcata acgtggtgat ttaccgcaac catattccgg cgcagacctt
    47821 aattgaacgc ctggcgacga tgagcaatcc ggtactgatg ctttctcctg gccccggtgt
    47881 gccgagcgaa gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat
    47941 tattggcatt tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca
    48001 ggcgggcgaa attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt
    48061 tgccggatta acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat
    48121 tccggccggt ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga
    48181 tgcggatcgc gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc
    48241 tcgcctgctg gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct
    48301 gcaaccgatt ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca
    48361 gctgttttca gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt
    48421 gagcatgaaa attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact
    48481 ggaaaacgca gcgccgttcc cgcgcccgga ttatctgttt gctgatatcg tcggtactgg
    48541 cggtgacggc agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgcggcctg
    48601 tgggctgaaa gtggcgaaac acggtaaccg cagcgtctcc agtaaatccg gctcgtcgga
    48661 tctgctggcg gcattcggta ttaatcttga tatgaacgcc aataaatcgc gccaggcgct
    48721 ggacgagtta ggcgtatgtt tcctctttgc gccgaaatat cacaccggat tccgccacgc
    48781 gatgccggtt cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat
    48841 taacccggcg catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc
    48901 gattgccgaa accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg
    48961 gatggatgaa gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcaaaat
    49021 taagagctat caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact
    49081 ggcaggcgga acacctgaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg
    49141 cgacgccgcc catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg
    49201 ccatgaagat ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc
    49261 ttacgacaga gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa
    49321 aatcgtcgca gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag
    49381 ttttcagaat gaggttcagc cgagcacacg acatttttat gatgcgctac agggtgcacg
    49441 cacggcgttt attctggagt gtaaaaaagc gtcgccgtca aaaggcgtga tccgtgatga
    49501 tttcgatccg gcacgcattg ccgccattta taaacattac gcttcggcaa tttcggtgct
    49561 gactgatgag aaatattttc aggggagctt tgatttcctc ctcatcgtca gccaaatcgc
    49621 cccgcagccg attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg
    49681 ctattaccag gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg
    49741 ccagcttgca gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga
    49801 agaggaactg gagcgcgcca ttgcattagg ggcaaaggtc gttggcatca acaaccgcga
    49861 tctgcgtgat ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca
    49921 caacgtaacg gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag
    49981 ccacttcgct aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc
    50041 cgccgtgcgc cgggtgttgc tgggtgagaa taaagtatgt ggcctgacgc gtgggcaaga
    50101 tgctaaagca gcttatgacg cgggcgcgat ttacggtggg ttgatttttg ttgcgacatc
    50161 accgcgttgc gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta
    50221 tgttggcgtg ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc
    50281 gctggcggca gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga
    50341 agctctgcca gcacatgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc
    50401 ccgcgagttt cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca
    50461 acgttttgac tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg
    50521 cttaggcgca gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa
    50581 ttctgctgta gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca
    50641 gacgctgcgc gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt
    50701 gagtttggcg gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa
    50761 gcttttgtca gcgcgcaaaa agatcctgaa tttcaagccc agttcaacga cctgctgaaa
    50821 aactatgccg ggcgtccaac cgcactgacc aaatgccaga acattacagc cgggacgaac
    50881 accacgctgt atctcaagcg tgaagatttg ctgcacggcg gcgcacataa aaccaaccag
    50941 gtgctggggc aggcgttgct ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc
    51001 ggggccggtc agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc
    51061 cgtatttata tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc
    51121 ttaatgggtg cggaagtgat cccggtacat agcggttccg ccacgctgaa agatgcctgt
    51181 aatgaggcgc tacgcgactg gtccggcagt tatgaaaccg cgcactatat gctgggcacc
    51241 gcggctggcc cgcatcctta tccgaccatt gtccgtgagt ttcagcggat gattggcgaa
    51301 gaaaccaaag cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt
    51361 gttggcggcg gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc
    51421 ggcctgattg gtgtggagcc tggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg
    51481 ctaaaacatg gtcgcgtggg catttatttc ggtatgaaag cgccgatgat gcaaaccgaa
    51541 gacgggcaga ttgaagaatc ttactccatc tccgccggac tggatttccc gtctgtcggt
    51601 ccacaacacg cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat
    51661 gaagcccttg aagccttcaa aacgctgtgc ctgcacgaag ggatcatccc ggcgctggaa
    51721 tcctcccacg ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga taaagagcag
    51781 ctactggtgg ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt
    51841 ttgaaagcac gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg
    51901 agcgcaaaga aggcgcattc gttcctttcg tcacgctcgg tgatccgggc attgagcagt
    51961 cattgaaaat tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggtatcc
    52021 ccttctccga cccactggcg gatggcccga ccattcaaaa cgccactctg cgcgcctttg
    52081 cggcaggtgt gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc
    52141 cgaccattcc cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg
    52201 agttttatgc ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccag
    52261 ttgaagagtc cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca
    52321 tctgcccgcc aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt
    52381 acacatattt gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc
    52441 tcaatcatct ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg
    52501 gtatttccgc cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt
    52561 ctggttcggc cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg
    52621 cggcactgaa agtttttgta caaccgatga aagcggcgac gcgcagttaa tcccacagcc
    52681 gccagttccg ctggcggcat tttaactttc tttaatgaag ccggaaaaat cctaaattca
    52741 tttaatattt atctttttac cgtttcgctt accccggtcg aacgtcaact tacgtcattt
    52801 ttccgcccaa cagtaatata atcaaacaaa ttaatcccgc aacataacac cagtaaaatc
    52861 aataattttc tctaagtcac atattcctca ggtaattgtt aatatatcca gaatgttcct
    52921 caaaatatat tttccctcta tcttctcgtt gcgcttaatt tgactaattc tcattagcga
    52981 ctaattttaa tgagtgtcga cacacaacac tcatattaat gaaacaatgc aacgcaacgg
    53041 gagaaataac atggccgaac atcgtggtag ttcaggaaat ttcgccgaag accgtgagaa
    53101 ggcatccgac gcaggccgta aaggcggtca gcatagcggc ggtaatttta aaaatgatcc
    53161 gcaacgcgca tctgaagcgg gtaaaaaagg cggtcaacaa agcggtggta ataaatcagg
    53221 caaatcctga ttcaccgtta attattaata tcagctgata aagataattg catctgcggg
    53281 ccgtatggct cgcagtgatt tcactggaga aaaatatgaa tatgaagacc attgaagatg
    53341 tatttattca actgctttca gatacctaca gcgcagaaaa acaattaacc cgggcactgg
    53401 caaaactcgc aagagcaaca tcaaatgaaa aattaagtca ggcttttcat gcgcacctcg
    53461 aggaaactca tggacagatt gaacgaattg atcaagttgt ggagtcagaa tcaaatctga
    53521 aaattaagcg catgaaatgt gtggcaatgg aaggtcttat tgaagaagct aatgaggtca
    53581 tcgaaagtac cgagaaaaac gaagtgcgtg atgccgcact gattgccgca gcacagaaag
    53641 tcgagcatta tgagattgcc agttacggga cattagcgac gctggctgaa caattaggtt
    53701 accgtaaagc agcgaagctt ctgaaagaaa ccctggaaga agaaaaggcc accgacatca
    53761 aactgactga tctggccctt aataacgtaa ataagaaagc cgaaaataaa gcctgaaata
    53821 tgaattttaa cttttagtca ttttataaag aggacatttt catgaatcgt attgaacatt
    53881 atcatgactg gttacgtgac gcccacgcaa tggaaaagca agccgaatct atgcttgaat
    53941 ccatggccag ccgtatagat aattatcctg aactacgcgc tcgtattgaa caacatctta
    54001 gtgaaaccaa aaaccagatt gttcaactgg aaactattct tgatcgtaat gacatttcac
    54061 gttcagtcat taaagattcc atgagtaaaa tggctgcgct tgggcagtca atcggtggta
    54121 tattcccttc tgatgaaata gttaaaggct ctattggcgg atatgtcttc gagcaatttg
    54181 aaatcacctg ttacacctca ctattagcag cagcaaaaaa tgccggtgat acagtctcaa
    54241 ttccgatcat cgaagcgatt ttaaatgagg aaaagcaaat ggccgactgg ctgattcagc
    54301 atattccgca aacaactgag aaatttttaa ttcgttctga aactgatggc gtagaagcga
    54361 agaaataata ccctttatac catgtcctta ttgaccccgt atattacggg gtcgtttttg
    54421 tgcggaatta aaaacgatat cctgctgaga acataaacac ccacggatcg aggcgtacgc
    54481 tatcgtgttg ctgtgcaccg cctagcttat aattggcggt ggtatcgata tccatgtacc
    54541 acactgacat gttaaccaac cagtcacggt taatcagata atcaaccccc acctgcccgg
    54601 cagctcccca ggaatctttc agactgagat cggaaagccc cgcctctttg ccatgatcgt
    54661 taaatccatt atcaaagaag gtggtgtagt taataccggc cccaacgtaa ggacggaatt
    54721 tgctgctggc atcaccaaaa taccactgcg ccatcagtgt tggcggcaga tggtgaacgg
    54781 ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc gctgccagta
    54841 attccacacc aatgttgtcg gtcgccatat aagtaaacgt caagcccagt tgagtgttat
    54901 tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct gttggacgta
    54961 cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc atgcgcaaag gcactgccag
    55021 aaagaagagt tgttactgcc aaagccgcca ctgttaactt tttcatatcc gctccgtcgt
    55081 tatggttata aaaacgtgac gaatatacct acattggagt aacaaatgat cctacataga
    55141 tcacattaca tatgataatt caccatttat tgatctggat taatttagaa gttgcattga
    55201 gaatcctggt taatgtgatt taaatcaatt tttaaatatt ttctctacgc aaatattaac
    55261 tcgatcgcca cttgccgcgg tttgcccacg cctgaaggcc agcaattacc tccgctttac
    55321 aaagatatgc ttttccatac aagacctgat gctgccaccc gaggcttaac cagggtacaa
    55381 ttgcccgcta attaacacct gcataaaact caaggagagt gcatgtctat cacggcgcag
    55441 tccgtatacc gtgacaccgg aaatttcttc cgtaatcaat ttatgaccat tctgttggta
    55501 tcgttgctat gtgcgtttat cacagtggtg ttagggcatg ttttctcacc cagtgatgca
    55561 cagcttgcgc agctcaatga cggcgtgccc gttagcggca gtagtgggtt gttcgacctg
    55621 gttcagaata tgtcaccgga acagcaacaa attttgctgc aggcttcagc ggcgtccact
    55681 ttttcagaat taatcggtaa cgccattctc gccggaggcg taatattaat tatccagctg
    55741 gtgtctgcgg gtcagagagt cagtgcgctg cgagctattg gtgccagtgc gccgatattg
    55801 cccaagttat ttattctgat ttttctgact acccttttag tacagattgg catcatgctg
    55861 gtggtcgttc cgggaattat catggccatt ttactggccc tggcaccggt gatgctggta
    55921 caggacaaaa tgggcatttt tgcctcgatg cgtagcagta tgcggctgac ttgggcgaat
    55981 atgcgtctgg tggcacccgc agtactgagc tggttgctgg caaaaacact gttgctgctt
    56041 tttgcctctt cttttgccgc attaaccccg gaaattggtg ccgtactggc gaacaccttg
    56101 agcaacctga tttctgccat attgctcatc tatctgttcc gcctgtatat gttgattcgc
    56161 caataacctt aagcatttga ttacggaatc gtaaaatgaa gcagtttctt gattttttac
    56221 cgctggttgt ctttttcgcg ttttacaaga tttatgacat ctatgcggct actgcagcgc
    56281 tgatcgtcgc cacggcgatt gtgcttatct atagctgggt tcgctttcgt aaggttgaga
    56341 agatggccct gatcactttt gttctggtgg tcgtcttcgg tggcttgacg ctgttcttcc
    56401 acaatgatga gtttattaaa tggaaggtga cagtcattta tgctctgttt gcgggagccc
    56461 tgttagtcag ccaatgggtg atgaaaaagc cgctaattca gcggatgctg agtaaagaac
    56521 tcacgctgcc gcaaccggta tggtcgaagc taaatctggc ctgggctgtt ttctttatcc
    56581 tttgcggtct ggcaaacatc tacatcgcat tctggctgcc gcaaaatatt tgggtcaact
    56641 ttaaagtctt tggcctgacc gcccttacct taatcttcac attgttaagc ggtatctata
    56701 tctaccgcca catgccgcag gaagataaat cctaaccaga ctgccagtcc ttcggggctg
    56761 gcgctgtatc tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc tgtgattttc
    56821 cttttaagtc ggttttacca tgtctacaac acataacgtc cctcagggcg atcttgtttt
    56881 acgtacttta gccatgcccg ccgataccaa tgccaatggt gacatctttg gtggttggtt
    56941 aatgtcacaa atggatattg gcggcgctat tctggcaaaa gaaattgccc acggtcgcgt
    57001 agtgactgtg cgggttgaag gaatgacttt cttacggccg gttgcggtcg gcgatgtggt
    57061 gtgctgctat gcacgctgtg tccagaaagg gacgacatcg gtcagcatta atattgaagt
    57121 atgggtgaaa aaagtagcgt ctgaaccaat cgggcaacgc tataaagcga cagaagcatt
    57181 atttaagtat gtagcggttg atcctgaagg aaaacctcgc gccttacctg ttgagtaata
    57241 gtcaaaagcc tccggtcgga ggcttttgac tttctgctta ctgaatttcg gtggtgccgt
    57301 taattttaaa caggatattc accacaatcc cactgcctgg cttacccggc tcataacgcc
    57361 atctgcgcat cgcatttttc acctcacgct caaacatgtt cgcaggcttg gctgagagga
    57421 tttgtacgtt atccacgcga ccatccggcg tgacgtcaaa tttaacttta acctgccctt
    57481 caatgcgcaa tgcctgtgct cgtgccggat actgcggctg attacggctt aatgcgcgtg
    57541 gtcctgaagc cacactggta accggcttgc tggttgcagc cgttgccgta cttgatgtgg
    57601 ggcgcgcggg tgccgtattt tcaaacggtg atgccggacg cgactctacg ggtttgacat
    57661 cgcgttttgg ctgctcctgt acctttttca ccggcttcgg ttttggcttc ggtttcggct
    57721 tcggcttttc aatgaccacc ggtgcttctt ttggcggttc ggggatcggc tcaggttccg
    57781 gttctggctc taccaccggc tccggtggcg gctgaacggc ttgtggcggt tcgagatcag
    57841 caggcgcaac catcgtgaca gaaattggtt gcgcaggcgc aggtagttca ataacctgat
    57901 gtaccgaggt atagagcagc cccgccacaa cagcaccatg aatgcagacc gaaagtaacg
    57961 tcggccaggg gaagcggcga ggtaaatcaa gggttattga agtcataatc atttcagtag
    58021 aaaaactagg cctcgatttt aaatgcaaat agcaatcata ttcaataagg caagacgatc
    58081 aggatcagtg aaaatgtcat ttttggcgta attaccctta ctttatgcat gaccttaaca
    58141 aagacatcat ctttccgttg cagatcttcg ccctttcaaa taacgtactt tacaactttc
    58201 ccgaacaagg agttgtgccc gtgttgtatg ttatttacgc tcaagacaaa gctgatagcc
    58261 tcgaaaaacg cctttccgtt cgtccggcac atttagcacg tttacagtta ctgcatgatg
    58321 aaggtcgttt gttgacagct ggtccaatgc cagcggtaga cagtaacgat ccaggggctg
    58381 cgggatttac tggctcaacc gttattgctg aatttgaatc gctggaagca gctcaggcct
    58441 gggccgatgc tgacccgtac gtagcggcag gagtctatga acacgtttca gtgaaaccat
    58501 ttaagaaagt gttctgagta
//
LOCUS       DAEEDS010000015        58087 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEEDS010000015 DAEEDS010000000
VERSION     DAEEDS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58087
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.015"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            705..1133
                     /gene="uspC"
                     /locus_tag="KLH32_000974"
     CDS             705..1133
                     /gene="uspC"
                     /locus_tag="KLH32_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBE0137910.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1140..2554)
                     /gene="otsA"
                     /locus_tag="KLH32_000975"
                     /pseudo
     CDS             complement(1140..2554)
                     /gene="otsA"
                     /locus_tag="KLH32_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2529..3329)
                     /gene="otsB"
                     /locus_tag="KLH32_000976"
     CDS             complement(2529..3329)
                     /gene="otsB"
                     /locus_tag="KLH32_000976"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBE0137911.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3496..4482)
                     /gene="araH"
                     /locus_tag="KLH32_000977"
     CDS             complement(3496..4482)
                     /gene="araH"
                     /locus_tag="KLH32_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBE0137912.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4497..6011)
                     /gene="araG"
                     /locus_tag="KLH32_000978"
     CDS             complement(4497..6011)
                     /gene="araG"
                     /locus_tag="KLH32_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBE0137913.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6081..7070)
                     /gene="araF"
                     /locus_tag="KLH32_000979"
                     /pseudo
     CDS             complement(6081..7070)
                     /gene="araF"
                     /locus_tag="KLH32_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7867..8370
                     /locus_tag="KLH32_000980"
     CDS             7867..8370
                     /locus_tag="KLH32_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBE0137914.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8450..8701)
                     /locus_tag="KLH32_000981"
     CDS             complement(8450..8701)
                     /locus_tag="KLH32_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBE0137915.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8816..8902)
                     /gene="azuC"
                     /locus_tag="KLH32_000982"
     CDS             complement(8816..8902)
                     /gene="azuC"
                     /locus_tag="KLH32_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBE0137916.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9165..9488
                     /locus_tag="KLH32_000983"
     CDS             9165..9488
                     /locus_tag="KLH32_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBE0137917.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9659..10156
                     /gene="ftnA"
                     /locus_tag="KLH32_000984"
     CDS             9659..10156
                     /gene="ftnA"
                     /locus_tag="KLH32_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBE0137918.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10193..10432)
                     /locus_tag="KLH32_000985"
     CDS             complement(10193..10432)
                     /locus_tag="KLH32_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBE0137919.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10622..11833
                     /gene="tyrP"
                     /locus_tag="KLH32_000986"
     CDS             10622..11833
                     /gene="tyrP"
                     /locus_tag="KLH32_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBE0137920.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11895..12560)
                     /gene="yecA"
                     /locus_tag="KLH32_000987"
     CDS             complement(11895..12560)
                     /gene="yecA"
                     /locus_tag="KLH32_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBE0137921.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12756..12842)
                     /locus_tag="KLH32_000988"
     tRNA            complement(12756..12842)
                     /locus_tag="KLH32_000988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12806..12808),aa:Leu,seq:taa)
     gene            complement(12855..12928)
                     /locus_tag="KLH32_000989"
     tRNA            complement(12855..12928)
                     /locus_tag="KLH32_000989"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12894..12896),aa:Cys,seq:gca)
     gene            complement(12983..13058)
                     /locus_tag="KLH32_000990"
     tRNA            complement(12983..13058)
                     /locus_tag="KLH32_000990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13023..13025),aa:Gly,seq:gcc)
     gene            complement(13210..13758)
                     /gene="pgsA"
                     /locus_tag="KLH32_000991"
     CDS             complement(13210..13758)
                     /gene="pgsA"
                     /locus_tag="KLH32_000991"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBE0137922.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13815..15647)
                     /gene="uvrC"
                     /locus_tag="KLH32_000992"
     CDS             complement(13815..15647)
                     /gene="uvrC"
                     /locus_tag="KLH32_000992"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBE0137923.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15644..16300)
                     /gene="uvrY"
                     /locus_tag="KLH32_000993"
     CDS             complement(15644..16300)
                     /gene="uvrY"
                     /locus_tag="KLH32_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBE0137924.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16596..16772
                     /locus_tag="KLH32_000994"
     CDS             16596..16772
                     /locus_tag="KLH32_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137925.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16759..16983
                     /locus_tag="KLH32_000995"
     CDS             16759..16983
                     /locus_tag="KLH32_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBE0137926.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17016..17636)
                     /gene="sdiA"
                     /locus_tag="KLH32_000996"
                     /pseudo
     CDS             complement(17016..17636)
                     /gene="sdiA"
                     /locus_tag="KLH32_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17866..18618)
                     /gene="yecC"
                     /locus_tag="KLH32_000997"
     CDS             complement(17866..18618)
                     /gene="yecC"
                     /locus_tag="KLH32_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBE0137927.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18615..19283)
                     /gene="tcyL"
                     /locus_tag="KLH32_000998"
     CDS             complement(18615..19283)
                     /gene="tcyL"
                     /locus_tag="KLH32_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBE0137928.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19298..20284)
                     /gene="dcyD"
                     /locus_tag="KLH32_000999"
     CDS             complement(19298..20284)
                     /gene="dcyD"
                     /locus_tag="KLH32_000999"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBE0137929.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20389..21189)
                     /gene="tcyJ"
                     /locus_tag="KLH32_001000"
     CDS             complement(20389..21189)
                     /gene="tcyJ"
                     /locus_tag="KLH32_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0137930.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21277..21828)
                     /gene="fliZ"
                     /locus_tag="KLH32_001001"
     CDS             complement(21277..21828)
                     /gene="fliZ"
                     /locus_tag="KLH32_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBE0137931.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21874..22593)
                     /gene="fliA"
                     /locus_tag="KLH32_001002"
     CDS             complement(21874..22593)
                     /gene="fliA"
                     /locus_tag="KLH32_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBE0137932.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22758..24410)
                     /gene="fliC"
                     /locus_tag="KLH32_001003"
     CDS             complement(22758..24410)
                     /gene="fliC"
                     /locus_tag="KLH32_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBE0137933.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24676..26082
                     /gene="fliD"
                     /locus_tag="KLH32_001004"
     CDS             24676..26082
                     /gene="fliD"
                     /locus_tag="KLH32_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBE0137934.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26107..26517
                     /gene="fliS"
                     /locus_tag="KLH32_001005"
     CDS             26107..26517
                     /gene="fliS"
                     /locus_tag="KLH32_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBE0137935.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26517..26882
                     /gene="fliT"
                     /locus_tag="KLH32_001006"
     CDS             26517..26882
                     /gene="fliT"
                     /locus_tag="KLH32_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBE0137936.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26960..28447
                     /gene="amyA"
                     /locus_tag="KLH32_001007"
     CDS             26960..28447
                     /gene="amyA"
                     /locus_tag="KLH32_001007"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBE0137937.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28481..28894)
                     /locus_tag="KLH32_001008"
     CDS             complement(28481..28894)
                     /locus_tag="KLH32_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0137938.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29081..30286
                     /gene="yedE"
                     /locus_tag="KLH32_001009"
     CDS             29081..30286
                     /gene="yedE"
                     /locus_tag="KLH32_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBE0137939.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30283..30516
                     /gene="yedF"
                     /locus_tag="KLH32_001010"
     CDS             30283..30516
                     /gene="yedF"
                     /locus_tag="KLH32_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBE0137940.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30623..31293
                     /locus_tag="KLH32_001011"
                     /pseudo
     CDS             30623..31293
                     /locus_tag="KLH32_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31333..32541)
                     /locus_tag="KLH32_001012"
                     /pseudo
     CDS             complement(31333..32541)
                     /locus_tag="KLH32_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32609..33224
                     /gene="tnpA"
                     /locus_tag="KLH32_001013"
                     /pseudo
     CDS             32609..33224
                     /gene="tnpA"
                     /locus_tag="KLH32_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            33552..34634
                     /locus_tag="KLH32_001014"
     CDS             33552..34634
                     /locus_tag="KLH32_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBE0137941.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(35123..35921)
                     /locus_tag="KLH32_001015"
                     /pseudo
     CDS             complement(35123..35921)
                     /locus_tag="KLH32_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(35931..36482)
                     /gene="ybcL"
                     /locus_tag="KLH32_001016"
     CDS             complement(35931..36482)
                     /gene="ybcL"
                     /locus_tag="KLH32_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBE0137942.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(36651..36983)
                     /gene="emrE"
                     /locus_tag="KLH32_001017"
     CDS             complement(36651..36983)
                     /gene="emrE"
                     /locus_tag="KLH32_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBE0137943.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(37328..37642)
                     /gene="fliE"
                     /locus_tag="KLH32_001018"
     CDS             complement(37328..37642)
                     /gene="fliE"
                     /locus_tag="KLH32_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBE0137944.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            37856..39521
                     /gene="fliF"
                     /locus_tag="KLH32_001019"
                     /pseudo
     CDS             37856..39521
                     /gene="fliF"
                     /locus_tag="KLH32_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            39514..40509
                     /gene="fliG"
                     /locus_tag="KLH32_001020"
     CDS             39514..40509
                     /gene="fliG"
                     /locus_tag="KLH32_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBE0137945.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            40502..41188
                     /gene="fliH"
                     /locus_tag="KLH32_001021"
     CDS             40502..41188
                     /gene="fliH"
                     /locus_tag="KLH32_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBE0137946.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            41188..42561
                     /gene="fliI"
                     /locus_tag="KLH32_001022"
     CDS             41188..42561
                     /gene="fliI"
                     /locus_tag="KLH32_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBE0137947.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWKASLQGLE
                     RIFPTVS"
     gene            42583..42924
                     /gene="fliJ"
                     /locus_tag="KLH32_001023"
                     /pseudo
     CDS             42583..42924
                     /gene="fliJ"
                     /locus_tag="KLH32_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            42921..44048
                     /gene="fliK"
                     /locus_tag="KLH32_001024"
     CDS             42921..44048
                     /gene="fliK"
                     /locus_tag="KLH32_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBE0137948.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            44153..44617
                     /gene="fliL"
                     /locus_tag="KLH32_001025"
     CDS             44153..44617
                     /gene="fliL"
                     /locus_tag="KLH32_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBE0137949.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            44622..45626
                     /gene="fliM"
                     /locus_tag="KLH32_001026"
     CDS             44622..45626
                     /gene="fliM"
                     /locus_tag="KLH32_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBE0137950.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            45623..46036
                     /gene="fliN"
                     /locus_tag="KLH32_001027"
     CDS             45623..46036
                     /gene="fliN"
                     /locus_tag="KLH32_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBE0137951.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            46039..46404
                     /gene="fliO"
                     /locus_tag="KLH32_001028"
     CDS             46039..46404
                     /gene="fliO"
                     /locus_tag="KLH32_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBE0137952.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            46404..47141
                     /gene="fliP"
                     /locus_tag="KLH32_001029"
     CDS             46404..47141
                     /gene="fliP"
                     /locus_tag="KLH32_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBE0137953.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            47151..47420
                     /gene="fliQ"
                     /locus_tag="KLH32_001030"
     CDS             47151..47420
                     /gene="fliQ"
                     /locus_tag="KLH32_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBE0137954.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            47429..48214
                     /gene="fliR"
                     /locus_tag="KLH32_001031"
     CDS             47429..48214
                     /gene="fliR"
                     /locus_tag="KLH32_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBE0137955.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            48499..49122
                     /gene="rcsA"
                     /locus_tag="KLH32_001032"
     CDS             48499..49122
                     /gene="rcsA"
                     /locus_tag="KLH32_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBE0137956.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(49166..49354)
                     /gene="dsrB"
                     /locus_tag="KLH32_001033"
     CDS             complement(49166..49354)
                     /gene="dsrB"
                     /locus_tag="KLH32_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBE0137957.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            49517..49744
                     /gene="yodD"
                     /locus_tag="KLH32_001034"
     CDS             49517..49744
                     /gene="yodD"
                     /locus_tag="KLH32_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBE0137958.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            50042..50857
                     /locus_tag="KLH32_001035"
                     /pseudo
     CDS             50042..50857
                     /locus_tag="KLH32_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(50854..52548)
                     /gene="yedQ"
                     /locus_tag="KLH32_001036"
                     /pseudo
     CDS             complement(50854..52548)
                     /gene="yedQ"
                     /locus_tag="KLH32_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(52719..52901)
                     /locus_tag="KLH32_001037"
     CDS             complement(52719..52901)
                     /locus_tag="KLH32_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBE0137959.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(52980..53897)
                     /locus_tag="KLH32_001038"
     CDS             complement(52980..53897)
                     /locus_tag="KLH32_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBE0137960.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            54070..54990
                     /gene="yedA"
                     /locus_tag="KLH32_001039"
     CDS             54070..54990
                     /gene="yedA"
                     /locus_tag="KLH32_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBE0137961.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(54979..55449)
                     /gene="vsr"
                     /locus_tag="KLH32_001040"
     CDS             complement(54979..55449)
                     /gene="vsr"
                     /locus_tag="KLH32_001040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBE0137962.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(55430..56848)
                     /gene="dcm"
                     /locus_tag="KLH32_001041"
     CDS             complement(55430..56848)
                     /gene="dcm"
                     /locus_tag="KLH32_001041"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBE0137963.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(56915..57455)
                     /locus_tag="KLH32_001042"
                     /pseudo
     CDS             complement(56915..57455)
                     /locus_tag="KLH32_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(57495..57752)
                     /locus_tag="KLH32_001043"
     CDS             complement(57495..57752)
                     /locus_tag="KLH32_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137964.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            complement(57753..>58087)
                     /locus_tag="KLH32_001044"
     CDS             complement(57753..>58087)
                     /locus_tag="KLH32_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBE0137965.1"
                     /translation="RNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDN
                     AVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQ
                     YYQRLGSV"
BASE COUNT        14608 a        14866 c        13967 g        14646 t
ORIGIN      
        1 aaaataatta gcattagaat agttgcgata agctgcaata agcagagcca cctttttggt
       61 ttaataagtc cttacaaata gaagggggtc tttacactta tctaagattt ttcctaaatc
      121 gacgcaactg tactcgtcac tacacgcaca tacaacggag gggggctgcg attttcaata
      181 atgtgtgatg cagatcacac aaaacactca attacttaac ataaatgtat aagtcatact
      241 tttgttttgg gtgtatttca atctgttaaa aagtttttcg ctacgctagc aagcaaaaat
      301 gaaacaggaa taatcgaaat gggatgttgc gcacggttaa aataactcac cgtaaataat
      361 catctgctat aaataatcac tttcatgcaa taccagataa gctattttta aacagacact
      421 taccgcacaa caaactaatt aaatacaaag aaaggcaaca atcaacttaa tgttaataaa
      481 atgtttttca tatattaacc acttgtttga tgcggggaat cagtacatta ttttcttata
      541 attacagttg aaatcatatg atcaccgaga gtgattgatt aattgtcttt actaataatc
      601 agactaatat ttacctgttt gactgagttg ggattgcgtc gtttctccat taggagtaaa
      661 gctttaatgt cacctgaagt tcacagcaga ataaggaaca ggttatgagc tatagcaata
      721 ttcttgtcgc tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta
      781 tcgccaggcc agtaaaggga cacatcagtt taattactct cgcttccaac ccggaaatgt
      841 acaatcaatt agccgcgccg atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc
      901 aaagctttct tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg
      961 cctacggtga gttaagcgaa catattctgg aagtatgtca caaacatcat ttcaatttgg
     1021 tgatttgcgg taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag
     1081 tgattgcctc aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc
     1141 tacgcaagct ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga
     1201 actatctgct ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg
     1261 ataacgtcca gcatttctgc atgacgggaa atacgttcca ccagcgacat agtcaatgca
     1321 cgatccagcg cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt
     1381 aactcgtttg ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc
     1441 tgagcagcaa catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag
     1501 cccacgtcag agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa
     1561 taataaagcg gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt
     1621 tcgagctgat gacgaatatc ctggtaggct tgcacatcac cacgcgacgt tggtgcaatc
     1681 tgggtataac gaattttacc atgatgctgc ggatattttt ccagcaatgc ctcataggcg
     1741 agaaaacgct ccggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt
     1801 acgtttttca attccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt
     1861 ttggctattt ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgcttttccc
     1921 caggctgtat ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aaggcaatcc
     1981 aggaacgcca gacgatcgtt ttctgtctgt aaacccagca aatcataatc acaaagctgt
     2041 tcaagcaagg tgtcatatgt cggcagcgcg ttgaaaattt ccggtgtcgg gaaaggaata
     2101 tgcaagaaaa aaccaatgcg attattcacc ccacgtttgc gtaattcatg cgcaaagggc
     2161 aacaggtgat aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta
     2221 tctgccagca acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc
     2281 agatcgagcc gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat
     2341 tcgtcaaggt cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttagcg
     2401 gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca
     2461 gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg
     2521 caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt
     2581 ttttgttgta atgcggcggt tatcatttca agccagctcc agacatccgg cacacccgcc
     2641 agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg
     2701 ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc
     2761 ccgataaaag gagcttcctg cataaaggct gcaatcgctt cacctttact ggtacctctc
     2821 ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta
     2881 atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc
     2941 gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat gaccgtatgc
     3001 agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca
     3061 ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc
     3121 agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg
     3181 gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc
     3241 ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt
     3301 tcaggggttt cggttaacgg ttctgtcacc cggctctcct ccttctcttt tcttattgtg
     3361 ttgcgcagac cgcgctttac agacatgtaa gtatagacag tgtgacggag gtcgccattt
     3421 gcaacgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg
     3481 ttgtggaaaa aagcatcaga cagtgcgctt cgctttttgc ttgtaacggt cgaagatcac
     3541 cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag
     3601 gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt
     3661 tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa caataagctc
     3721 ataaccaatc gacgtcattg gctgcccact agtcatacgt gaagccagaa taattccggc
     3781 tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa caaccggtat
     3841 acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtattac gaccaaaggt
     3901 ggttttattc agcagcaaac caaagataat cagacacgcg acggtgagcc agattggcgc
     3961 aggcagaccg aaccagttag cgtaaccaag ggcaaagaag ctttcatctt cgataccgac
     4021 cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg ttgccagtgt
     4081 cgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca ggccacagag
     4141 aacgcccagc aacaaacccg ctgccacgcc aatccacagg ctttcagtca ggttgataac
     4201 caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt caaagtcacc
     4261 ggaagcaagg cagaacaaca tgccacacgc caccatcccc gacatggaaa ttgccaggcc
     4321 caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac aggcgataaa
     4381 gagcaccgca aacaccacca gcatgccgta ctgatcccag atacgcccga agctgaatga
     4441 cgacttaggt gcgccagacc ccgatgtaga aacagaagac atcatactct ccttactcag
     4501 gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc atctgcctgc
     4561 tcgtgtaaca attcaccggc gatttcgcct tcccgcatca ccacaatccg gtcggcaacg
     4621 ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacgccctg cgccgccagc
     4681 gcataaatta cgttatatat ttcgtgctta gcgccaacat caatgccgcg cgtaggttca
     4741 tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc tttttgctga
     4801 tttccgcctg agagattcat gatcagttgc tccgcgcccg gcgttttgat gttgagcgaa
     4861 cgaatgtgct gatcggcatt gttttcttcc caactgttgt tgattacaca accgccgagc
     4921 acatgtttgc gtctggcact gatgttgata ttgtcgcgaa cggagtgcac gggaataatg
     4981 ccttccgctt tgcgagcttc cgggcagagc atcatgcctg cggcaatggc gtggctcggt
     5041 ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat ttgcgtcccg
     5101 ccaaacaagc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa cagcccaaca
     5161 atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctagtgc tttcacagca
     5221 tcaagacgca ggcgctcctc gccataacta cgaggttgcc agccgtagat atcgccaatg
     5281 tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag
     5341 gttttgacat aacgtccatc tttaaagaca gtgatggcat cgctgagggc aaatatttct
     5401 tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc acgaataacg
     5461 cggaaaagat tgccgatttc gcgggcagaa agggagctgg ttggctcatc aaaggcgata
     5521 attttagcgt tacgcgccag cgctttggcg atttcaacca tctgccactg accaatggcg
     5581 agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag ttgtaaaccc
     5641 gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag ctggccgaga
     5701 tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta aataatcgcc
     5761 acacccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact
     5821 acagaacccg tggttggcgc atagttgccg ctgaggattt ttaagagagt tgattttcct
     5881 gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa actaatatcc
     5941 gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag atacggggta
     6001 gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg catcatgcag
     6061 aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag ttcttcttta
     6121 aagttgtcac gcgtgatcag taccacgtct gtaacttcgg taaattttgg cgtttcaacg
     6181 tcttttgcta cccagttgta aagcatttcg ctggatttat agccatgtac gtccgggctt
     6241 ggcagcaggg aaccgtagaa gccggttgcc tgtgctttag acagttcgct tactgcatcc
     6301 acaccgttaa taccaatacc gatgatatcg gctgctttaa agccctgacc ttcggttgca
     6361 cgtacgccgc caagtacggt gctgtcgttc ataccgacga tcagccaatg tttaacttcc
     6421 ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc gttagatttg
     6481 gtaggtacct gataaatttg tttttccggg aatcctgcag ctttcagtgc gtccatagaa
     6541 cccgttgtac ggcggcgggc ggtatccagt tcgttggcgg taatcgccat cactgcgctt
     6601 tctttgacat cccagccacg tttctgcatc tctttataca gttcctagcc ctgacgttcg
     6661 ccaattttag tcgccgccat catcaccaga ggaacggtat ccattggctt acctttggcg
     6721 ttaacaaact ggtcatccac tgcaatgact ttcatatcgt agccacgcgc tttcgcgacg
     6781 atggcagagc cgagtttcgg gtccggagta caaataacga aaccttttgc gccactggct
     6841 gccaggctgt cgatcgcgtt caatgttttt tcgccatccg gcacggcaat cttaataacc
     6901 tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg gaaccacggc
     6961 tcttccggtt gcttcaccag aaaaccgagc ttcaggttct ccgccatagc ggattgtgac
     7021 ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat ggttctctcc
     7081 agctttagtg tcgttttgtg tagtgcaaaa acgaatgaca ttcgttaaat taatcggaaa
     7141 acaaagcatt accttttatc taaaagataa gtgaccgtgt tgacatagtt ttagcgagaa
     7201 attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa cgtgcataaa
     7261 tttagcggga aaagacataa ggaaaagcca atttgtcaga caaattgttg aatgcacagc
     7321 agattaatcc ataagattag cctggaaatc cttgttgtct tcggtaccca ttcgggatgt
     7381 cttcttttta accagtcaat aggccgcatt acctggcgtt gagtttttga aatggtgtaa
     7441 taaccacaac tcaaagatgt ggaaaatgcg cgtcattcat ttcgccatta attatcactg
     7501 tgctcattaa ttaacagaac acgtataatg agagccatct cgcaaaaatg aaaaaacgtt
     7561 ttataaaatc atcacttcat catgaattca aattcatcga ttaatatcaa caagatacaa
     7621 aaagcactat cattaaaatt cattgcattt acattgattt caccaatgaa atgtaaaaat
     7681 atataaactt gatgatttaa gcattttctt ataaccgttc agacgttatt cttatttcag
     7741 atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga attattctgc
     7801 aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga tataaggata
     7861 ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa ccgcgagttt
     7921 tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag tctgaacggc
     7981 accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat gcgcatgttt
     8041 aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt tccaggtgaa
     8101 aaactgaact ctctggaaga actgttccaa aaaacgatgg aagaatacga gcaacgttca
     8161 agcactctgg cgcagttagc cgatgaagcg aaagaactga atgatgattc aaccgtcaat
     8221 ttcctgcgcg atctggaaaa agaacagcag catgatggcc tgttgctgca aaccattctt
     8281 gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga ccaacatgtt
     8341 ctgaatgtcg tgtcacacca gctgcattga tcatacatcg gcgctaatac attgcgccga
     8401 tgaagttttt gagaaaccgc tgcctcatct gtttgaagca gcggtttttt taatgggatt
     8461 caccctgtgg ggtaaacttg agttcaatca gcgcgatggc tttttggatt gcccgcatgg
     8521 tgaccgggtc tgcggcagcg ggatgatgag taaagtcgat attcttcagc tgactggaca
     8581 ttttttcacg aacttcaacg ggcgcgatta catcgagaac atccagaatt tgtttgataa
     8641 ccaactggca agctaccaca tcagaaacca gttcctgatc ggcattcagc ggctgggaca
     8701 tcgtaaactc ctgatagcat tttgaaagcc gttatagtag cggcttcaca tcttcagcga
     8761 tagtcacatc caccgtcatc aggacacaaa aaaacctgcc ggagcaggtt ttttgttatc
     8821 ggaacatatt gcctggcggt acgtctttga acgtcttgca atagttattg aacatacttt
     8881 tcaggatttt gcgcagtttc atcgcggcac tccgaccatt tgttatacag gtgttattgt
     8941 ctttgcgctt ataatatgac aaccatcaca aaaatcaatc tttatgtgat acaaatcaca
     9001 taaatacccc tttaatgtta taaaaatgat aatcaaaaaa cagcccccct atttctgaca
     9061 cctacagatg gcaagaaata gcgcctgcca ggcgtctttt ccggccattg tcgcagcgct
     9121 gtaacgcgta aaatagtgct ttctcttact cttctggctg gaccatgaga cttctgattc
     9181 tgactctttc attaataacg ctggcggggt gtacggttac tcgccaggcc catgtgagcg
     9241 aagttgacgc ggcaacaggt attgtacggt tggtttatga ccaggctttt ttgcagcatg
     9301 cccataccga tcgttatgtc agtcgcggga tcgccgatcg cgcatgccag caggaaggct
     9361 atacccacgc gatccccttt ggtcagccag taggcaactg tagccttttt gcgggttcct
     9421 tatgtttgaa tacggaattc actttgtcat atcagtgcca tcattccgcc ttccctgtct
     9481 ttctctaaat attttaatca gcgaggggat cttcgctgat taaagaaata gcggtaatgc
     9541 gttttattcc cattcgtatt tttaataatt gaagtttata ttttaccttt tgcaaataat
     9601 aaaataacaa attatagtga cgccacagca acaaatataa cctttgtgga gcactatcat
     9661 gctgaaacca gaaatgattg aaaaacttaa tgagcagatg aacctggaac tgtactcttc
     9721 actgctttat cagcaaatga gcgcctggtg cagctatcat accttcgaag gtgctgccgc
     9781 gttcctgcgc cgtcacgccc aggaagagat gacgcatatg cagcgtctgt ttgattacct
     9841 gactgatacc ggcaatttac cgcgtattaa taccgttgaa tctccgtttg ctgaatattc
     9901 ctcacttgat gaattattcc aggaaaccta taaacacgaa caattaatca cccagaaaat
     9961 taacgaactg gctcatgctg caatgaccaa tcaggactac ccaacattta atttcctgca
    10021 gtggtatgtt tctgagcagc atgaagaaga gaaactgttc aaatcgatta ttgataaatt
    10081 aagcctggca ggcaaaagcg gcgaaggtct gtattttatc gacaaagaac tctctaccct
    10141 cgacacacaa aactaatgct ctccggcggc aggtttctgc cgccgtttca aatcagtgac
    10201 ggcaaatctt actctgatcg gtcgaaaaac cttcttctga aggtataaat ttgccttttg
    10261 ctgccagaaa cgctaccagc tctcccgctg tcaccccttc tgccgagcac gtgtgaaaac
    10321 gtgcctgttc accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct
    10381 cgcctgattc aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc
    10441 ctcaaaaaaa gcctagcgta gcgattaccg cttatgaaga ctttgcgcca gcgcaggact
    10501 gaatgctttt attgtacatt tatatttaca caatatgtaa cgtcggtttg acgaagcagc
    10561 cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag
    10621 cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg
    10681 catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt taatcttgtt
    10741 gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg tgtaccagca
    10801 tgttccggca gataccggtc tgggcacgct ggcaaaacgc tatctgggac gctacggtca
    10861 atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcag catacatcag
    10921 cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc
    10981 aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg ttggaacatc
    11041 gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc tggtggtgat
    11101 gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca
    11161 ggggctggca ctgtctgcaa tcccggtgat ttttacgtcg tttggttttc acggtagcgt
    11221 gccgagtatt gtcagctata tggatggcaa cattcgtaag ctacgctggg tgtttataat
    11281 cggtagtgcc atccccctgg tggcatatat tttctggcag gtggcgacgc ttggcagcat
    11341 tgattcaaca acctttatgg gattgctggc taatcatgct ggattaaacg ggctgttaca
    11401 ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcggtgcatt tatttgctga
    11461 tttagccctc gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt atctggctga
    11521 tttgtttcag cgttcaaata ccgttggtgg acgattgcaa actggtgcaa ttacctttct
    11581 gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc
    11641 cggtgtggcg ctggcggtac tggcattgat tatcccttca ctattgacct ggcaaagcag
    11701 aaagcacaat cctcaggcgg gttaccgggt caaaggtggt cgtccggcgc tggtggtggt
    11761 gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag gcttgttacc
    11821 agaagtgggg tgatcagata gcctcaaatt ccttattggg tgccagaatt aacgctgaca
    11881 cccaatgtga cctcttaatg caggcagcac tgcttaaatt tcttaccact gccgcacgga
    11941 caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac agctttttcc
    12001 tgcggatgcg ccatccagta tgcatgtaaa tcaagcgccg ccagtcgaat ggcatctacg
    12061 ctctcttcaa acgcttctgg cgacattttc tctacccgct cgaagttttc ctcagtaccg
    12121 tgcagcgcaa tcgcctccag cgctggtttt aaagaatcgg gcaacgttga ccagtcagaa
    12181 agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag ctcgctgcca
    12241 tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt cagacgctct
    12301 gcggtatcgg ccatatgttg aaaagccagg ttcataaagc gcgtcatctc tttctctgac
    12361 gcccaacgcg gcacatagtc agccccaccc cacacggcaa ccagccactg ttctggttca
    12421 atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc cacatcaagg
    12481 atggcgtggt cagtgttgta tttggtcaga atatcgtcca gccattccaa ctcactttcg
    12541 tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca gcacctatct
    12601 tacatgccgg tccgtatcag agatactttt tgagtggctt tgccggtgat taaaaattaa
    12661 ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt ttcacgacac
    12721 tgcttattac tttgatttta ttcttatctt tcccatggta cccggagcgg gacttgaacc
    12781 cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt ctaccgattc caccatccgg
    12841 gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg gactagacgg atttgcaatc
    12901 cgctacataa ccgctttgtt aacgcgccaa attcttcagg cctttcagcc agacatccgc
    12961 ttgacgccga tgtcttttaa actggagcgg gaaacgagac tcgaactcga gaccccgacc
    13021 ttggcaaggt tgtgctctac caactgagct attcccgcat tcatcaagca atcagttaat
    13081 cacttgattt tattatcgtc tggcaatcag tgccgccgtt cgatgcgttg cattctactt
    13141 acctggcgcg atgagtcaac gatatttttc accacttttg atcgtttgct gaaaattacg
    13201 ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg ctcaaatatt gcaacattga
    13261 ccacagagtc agtaccgcag ccacaaagaa aagtgcaata ccgacgtact caacccaaat
    13321 gttcggacgc cacagcagcc aggccaacgc caccatctgg gcagtggttt tcactttccc
    13381 aatccaggag acggccacgc tactgcgttt acccaactcc gccatccatt cgcgtagcgc
    13441 agaaataata atttcacggg cgatcatcgt tgccgccggt aatgtcaccc accagctgtg
    13501 ataatgctcg gttaccagca ccatggcgat agccacgaga actttatctg ccacagggtc
    13561 aaggaaagca ccaaaccggg tactctggtt ccagcggcgt gccagaaaac catcgaacca
    13621 gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca aacggcgacc aggtgacagg
    13681 cagataaaag accaatacaa agaatgggat aaggatgaca cggaacagtg taagcaacgt
    13741 agggatatta aattgcataa tgacgggtaa ctatctgttg tcagtaagat tacccctatg
    13801 ttgctacaga gacatcaatg tttcaacgac cagaagatct tttctgccag accttgcgaa
    13861 atacccggca cttttgcaat ttcctcgacg ctggcgttac gtaaaccttg caaaccgccc
    13921 atatatttca acaacatttg ccgacgtttt ggcccgacgc cttcaatggt ttccagggaa
    13981 ctggtatttt tgaccttcgc ccgtttttta cggtgcccgc caatcgcgtg atcatgtgat
    14041 tcatcgcgaa tatgctggat aacatgcagc gcgggtgaat ctggcggcaa actaaatccc
    14101 tcaccttccg gctcaaagaa cagcgtttcc agtccagcct tacgatctgc tcctttggca
    14161 acgccaagta gcagcggatg atttttatcc catgagacat ccagttcggc gaagacattt
    14221 ttcgcctgcg caagctggcc tttgccgccg tcgataagga tcacatccgg gatcttactg
    14281 tcgtcaatgg ctttaccata acgccgacgc agcacctgat tcatcgccgc ataatcatcg
    14341 cccggcgtga tgcctgtaat gttatagcgc cgatactccg cacgcagcgg gccgttagca
    14401 tcaaacacca cacaggaagc gacggtttgt tcgcccatgg tatggctgat gtcaaagcac
    14461 tccatccgct tcacttccgg caatttcaac acgctggcaa gcgcggtcag tcgctggtga
    14521 acggtagatt gctgcgaaag tttgctggtt aaggccgtcg ccgcattggt gcgcgcgagt
    14581 ttcagataac gcgccctatc gccgcgaggt ttggtttgaa cattaatctt gcgtcccgcc
    14641 agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa gattaaaatc gagcaggatc
    14701 tcacccggta aggtgcgcat ctggctgcct tgtaaataga actgacctac gaaggtttct
    14761 accacctcgc tcagttccgt accgccaggc actttcggga aatagctgcg gctgccgagc
    14821 actttgccct gacgaatgaa caatacgtgg acacaagcca tgcccgcatc gaacgccaca
    14881 ccaataacgt caaggtcgtc gccggtattg gaaacaaact gtttttcggt gacgcgtcgc
    14941 accgcctgaa tttggtcgcg aatacatgcc gcttcttcaa actccagatt ctggctggca
    15001 gtttccatac ggctaatgag ttgcgtaagc acctgatcat ctttgccaga caaaaacagg
    15061 cgcacatact cgacctgctg agcgtattct tcttcactca ccagtccttc aacgcacggt
    15121 cccagacagc gccctatctg gtattgcaga cacggacgcg agcgattgcg ataaacacta
    15181 ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg ccagtgtttc acgtacggca
    15241 tagccattcg ggaacgggcc gaaatattca cctttggcat gctttgcacc acgatgcatc
    15301 gccagacgcg ggtgggtatc gccactcagg aagataaaag gataagattt atcatcgcgt
    15361 agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt tgtgttccag caacagcgct
    15421 tcggtttctg tatgcgtaac cgttacatca atttgctgga tctgggcgac cagcgcttcg
    15481 gttttgcgcg aagcgaggtt gctgcggaaa tagctggaaa gccgtttttt caggtctttc
    15541 gctttgccga catagataac cgtaccacca gcatcgtaca tgcgataaac gcctggctgg
    15601 ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat cactcactga cttgataatg
    15661 tctccgcatt acacagacca tggcgaattg ccaggtgagt cagctcaaca tcgccatgaa
    15721 tgtttagttt actgaacata cgatagcggt agctgttcac cgttttcgga ctgagattga
    15781 gctgttctga gatctcattg accttctggc ccttagtgat catcagcata atctgcaatt
    15841 cacgttcaga caaactggca aatgggcttt ctgttttttc tggttcgatc tggcttaacg
    15901 ccatttgttg agcgatatca gaagcaatgt aacgctgccc tgaatagaca gaacgaatcg
    15961 cactcacgac ttcctgcgga gccgcgcctt tgctgaggta gccagcagca cctgcctgca
    16021 tgactttcgc tggtaaaggg ttttctgtat ggaccgtaag catgatgatt ttaacatcag
    16081 ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc aatgcccggc atactcatgt
    16141 ccattagcac cacgtcaacg gcatttgtcc ggcaccactt aacggcgtct tcaccgcacg
    16201 atgcctctcc ggcgactttt atacccttaa tatcttccag aatgcgtcgt atccctgcgc
    16261 gcaccagttc gtggtcatca acaagtagaa cgttgatcaa aggaatatct ccagaaatag
    16321 ggataacgct actgatagtt agtcattcgt atattagcgg tttttattgc aactttgaaa
    16381 cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg tcaattcgat tgtccacagt
    16441 tatggtcacg cccgtcacca acggcttacc ggatcgtttc acagtaagcc ctacgatgaa
    16501 atgtgacaaa aattgacttt attcagcaaa aatgaaaatc agcccgctga atatgtaaca
    16561 ttaattaacc cgaacaatac gggcaaaaaa cattcatgat aaaaatattt attggtcatt
    16621 atattaacgt attttatagc actgccgata tcacgctcaa aaaacaacca ctgctatttt
    16681 tagcaaagct tatggtatac tccgccgcct taacattttt caccacaaat tttcattgca
    16741 acatgacgag gaaaataaat gagtacgcct gatttttcta ctgccgagaa taatcaagaa
    16801 ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc tgatgctgca ggcgatggga
    16861 caagctgacg cgggccgcgt gatgttaaag atggaaaaac agcttgcgct gatcgaagac
    16921 gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag
    16981 taatataaaa ccggctgaga gcgtgccttt cagccggtta ttgacaaaat catcgctatc
    17041 tctgctgatg tcttcccttc cgccgtccat ttcagaattt ctttttcgcg cttgctgaaa
    17101 ttcatctctg gcgtcatcac tatttcatca tttaaacgca tcagagccat cagactttcg
    17161 cgcaccagta actgcatttt taattgcagt tcatcactaa gaatgggtat ttcgcgcgtg
    17221 ctgcaacggg aaaaggacaa aaagcccagc gcccgatttg gcagcattaa atactgagtg
    17281 acaccgcggc gtaaaccatg tgcgtgcgcg gcttcccata atggctgtgc ttcgctgaat
    17341 aagtcatcat tccacattaa atggccctga ctaaagtttt cagggttcag cacaggatca
    17401 attgcgagaa aattttttgc ctgataataa ctaacccacg actcagggta attggtgtag
    17461 aaagccactt taggtcgcgt gaatggtacc gggtggcgaa cacataacga atagtaatcg
    17521 tactccagct gctgagcctg aagctctatt tcatggtagg cctcttctgc ggcctccatc
    17581 ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa aatccgtatc ctgcatagta
    17641 aaccgcaacg cccctgagag tgagtatcat ttataatgat aattcaaact atcacataaa
    17701 taagatttat atataattta tattattcag gcaatgaatt acttttgcaa gccatcgcat
    17761 tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac gcttgaaatg
    17821 gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt gcagcagaag
    17881 cttctcgagg aactggcggg tgcgaggctg ctggggatcg gcaaataacg cttttgctgc
    17941 cccctgctcg actatccgcc cctggtccat aaagatcgcc cggtccgcaa catcccgggc
    18001 aaagctcatt tcgtgtgtca caatcaccat cgtgcgcttt tcctgcgcca gctggcggat
    18061 ggtgttcagg acttcgccca ccagctctgg atccagcgcc gacgttggct cgtcaaacaa
    18121 aatcacctca ggacgcattg ccagtgcacg cgcaatcgca acacgctgct gttgaccgcc
    18181 agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg ccagcagctc
    18241 gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg gcccttcaat
    18301 aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga caaacccgac
    18361 gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg cagtatcaat
    18421 agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat ttatgctacg
    18481 tagcaatgtg gttttgccgg aaccactcgg accgataatt gccaccactt cgccaggctt
    18541 tacctcgagg tcgataccgt gcagcaccgt ctgaccgtgg aattttttca ccaggttctt
    18601 aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt ctcaaaatgg
    18661 ttctgcaacg tcgataacac agtcgccatg atccagtaga tgagcgaagc cgccaggtac
    18721 atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa cagctccggc
    18781 acctggattg tcgcggccag cgacgtatct tttaccagac tgataaagct gttcgacagc
    18841 ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt ctgccacggc
    18901 gtcataccaa tactggcagc ggcttcccac tgacctttat caatagaaga aatggcggcg
    18961 cgcagcgttt cggcggcata ggcggcagtg ttcagcgaca gaccaatcat tgctgacgga
    19021 atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa cagttgtgcg
    19081 atgagcggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg cacgggccag
    19141 attggcgaca ggcgcatcag cgcgagaata aacccgagta gtaagccaaa aaacatgccg
    19201 ccgatactga gttgcagggt atacccagcc cctttcaaca ggaacggcaa ggaatcaata
    19261 accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga tgataggcga
    19321 acagcgcagg cgcgccgccg gtatgaataa acagaatcgg cccttcatct ttgaagcgtt
    19381 tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca ggatcaagca
    19441 gaatgccttc cagacgcgcc agcaatttca ctgcttccat gccttcgtcg ttcggcacgc
    19501 cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg gtcagctcca
    19561 gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caattgatcg gcaacggaac
    19621 gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc ccaacagcca
    19681 gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata ttaaccgccc
    19741 cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg ccaagagcat
    19801 tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact cgcgtcgcca
    19861 gttcttccag ttgggcattg ggatcggtca gtgcgtcgca catttcaatc tgggtattga
    19921 acagatccaa caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt gtgccaatag
    19981 gattttccag cagcgccacg cagtgcagac cgagtttcgc cgcgactgcg gcagtctggc
    20041 gcacatggtt agactggatc gcgccggcag taatcagcgt atcggcacct tcgcgcaggg
    20101 catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc atgggggtga
    20161 cctcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc ggcagatatt
    20221 cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa ttatgcagtg
    20281 gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct gaaaacgcgt
    20341 aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt atttggtcac
    20401 atcagcacca aaccattttt cggaaagagc ttgcagagtg ccgtcttttt gcatttccgc
    20461 aattgcatca ttcaccgctt tcagcaggtc ttcatttcct ttacgcagcg ccacgccaga
    20521 ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt tcttcaccag
    20581 atccagcgcc gccagacgat caacgaggat cgcatcgata cgccctacgc gcagatcctg
    20641 atatttggtc gggtcatcat cataggtacg cacatcgaca ccctgaacat tctgccgcag
    20701 ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca gatcagcagc
    20761 cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccgg aaatggtgta
    20821 aggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct gattaatcac
    20881 cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg tcggttttag
    20941 tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt caaaaccggt
    21001 taatttgccg tcatccccct gaaaactgaa cggcggataa gtgccttcca gccctaccag
    21061 cagcgtgccg ctctctttga ctttattaag cagaccttca tctgcaaaac ttttaacgct
    21121 catgcccgca accagcgcca cggccatcac acccatcaat gcctgacgtc ccagatgtgc
    21181 taatttcata ttcaccccga atgttgttat gtctgtttgc agtgtagagc cattgtttgt
    21241 aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag aagcaggcag
    21301 gctggaggat ttctgcacaa gtccggtaca ggtttggcgc tgatagtgtt gatacttccg
    21361 taatgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga ggatttcatc
    21421 aaggaaaccg tcctgcagct gatcgaggga aatattttgc tcatgtagat gattacccaa
    21481 acggcgcaga cgaacgacat attcacgcac cgtacctggg ctcatttcag tttgctcaaa
    21541 aagaaatttc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc
    21601 accgcaaaat cggcacaatg ccgcccatga ttgttgttcc gtttgccagc ctttttcatc
    21661 aagcagcgtg tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaactaa
    21721 ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc
    21781 tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg
    21841 gtagtcaaag ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa
    21901 ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcaccgc
    21961 gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca gtttttcgcg
    22021 ctccggcaac gtttcgatgg cttccatcac ccgctggcgc agattactgt ccagcagttg
    22081 ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat cgccgtgctc
    22141 ttcacgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca gcatttgacg
    22201 ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc
    22261 aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct
    22321 gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga tacgctgcac
    22381 tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacag cattaagtaa
    22441 cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg
    22501 caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc
    22561 cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt
    22621 atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag
    22681 gggttatttg ggggttacgc aaaaagccga atgtaaaaaa ccccgccggt ggcggggttt
    22741 gagaggcatt cagacgatta accctgcagc agagacagaa cctgctgcgg tacctggttg
    22801 gcttttgcca gcacggagtt accggcctgc tggataatct gcgctttcga catgttggac
    22861 acttcggtcg catagtcggc gtcctgaata cgggactgcg cttcagacag gttggtagtg
    22921 gtgttgttca ggttggtgac tgcggaatcc aaacggtttt gcaccgcacc cagggaggag
    22981 cggaatttgt cgatggagct gatggcttca tccagagctt tcaggggatc ggctgtcgta
    23041 gctgcttttg tttcagcatc tgtcgtcaat ttaccatcag catctttata aactgcctta
    23101 ccgctgccgt tagtcactga accatcatcc tgtaggtaaa aatctacagt atcagatgtg
    23161 gtcaacgcct cgttgttatc aacataaacc gaatcaacac cttttgttac agcgccagtt
    23221 gtgccgttga cgctataacc tgcagtagtg aaatcaacat tgtttcccga ggttgataca
    23281 gctttatcga aagctgcctg atcaactttt gcacctgtta ctgaataagt aatagtgtcc
    23341 ggcgtagtac tattaccggt aaaggagata gaggtaccgc caatgtcaat ggttgtcgct
    23401 ttggtattat tggctgcaag tgtggacaga ctagcctcag tcaaagaagc accagagccg
    23461 tttttcgtta agcgcccagt tgtatcaatg tagagtttat ctccattgct tgccgtaatt
    23521 ttaccatcac tgctgatctg aacatcctgt gcagaaccat cgatagtaat agtgccctta
    23581 gcggtatcac caactcccgg ggtcaaatat ttctgaacat cggcagctga agccgttgtg
    23641 gtatcaaaag agtatgactt acttgcactg tcatacttgt aattcgttgc actagccgcc
    23701 gttccgaagc cgttattaat ggttgcctga acagtatcac catcactaac tccagccagc
    23761 aaatcagacg ctttagcagc atcgtaaccc gcactcacag tatatttgtt gccgaccact
    23821 gttgcattag cagctaccaa gtcagcttta gatgcagcag tattagccac agccccgcca
    23881 ccattcacat taaacccatt cagccccagc gtatcagagt caattttctt cagatcaata
    23941 gtgatagtct ggccgtcatt cgcaccaacc tgaattttca tcgaaccgtc ttttgccagt
    24001 acgttcacgc cgttgaactg ggtctgaccg gatacgcggt caatttcgtc gagacgggat
    24061 ttgatttcgt cctgaatgga gtccagatcc gaatcagagt tagtcccggt agaagcctga
    24121 accgtcagct cacggatacg ctgtaagttg ttgttgattt cggacagcgc gccttcagtg
    24181 gtctgtgcaa cagaaatacc gtcgttggcg ttacgtgcag cctgagtcag gcctttaatg
    24241 ttagaagtaa aacggttagc aatcgcctga cccgcggcgt catccttcgc gctgttaata
    24301 cgcaagccag aagacagacg ctcgatagaa ctcgacagcg cagactggtt cttgttgata
    24361 ttattttgag tgatcagcga gaggctgttg gtattaatga cttgtgccat gattcgttat
    24421 cctatattgc aagtcgttga ttacgttttg ggtttctacc cgtcggctca atcgccgtca
    24481 accctgttat cgtctgtcgt aaaacaacct ttagaatttt tttcacaaac agccattttt
    24541 tgttagtcga cgaaatactc ttttctctgc cccttattcc cgctattaaa aaaaacaatt
    24601 aaacgtaaac tttgcgcaat tcaggccgat aaccccggta ttcgttttac gtgtcgaaag
    24661 ataaaaggaa atcgcatggc aagtatttca tcgctgggag tcgggtcagg tctggattta
    24721 agttcaatcc ttgatagcct caccgccgcg caaaaagcga cgctaacccc tatttcaaat
    24781 cagcaatcgt cgtttaccgc taaacttagc gcctacggta cgctgaaaag cgcgctgacg
    24841 actttccaga ccgccaatac tgcattgtct aaagccgatc ttttttccgc caccagcacc
    24901 accagcagca ccaccgcgtt cagtgccacc accgcgggta acgccatcgc cgggaaatac
    24961 accatcagcg tcacccatct ggcgcaggcg caaaccctga ccacgcgcac caccagagat
    25021 gatacgaaaa cggcgatcgc caccagcgac agcaaactca ccattcaaca aggcgacgac
    25081 aaagatccga ttaccattga tatcagcgcg gctaactcat cgttaagcgg gatccgtgat
    25141 gccatcaaca acgcaaaagc gggcgtaagt gcgagcatca ttaacgtggg taacggtgaa
    25201 tatcgtctgt cagtcacatc aaatgacacc ggccttgata atgcgatgac actctcggtc
    25261 agcggtgatg atgcgctaca aagttttatg ggctatgacg ccagtgccag cagcaacggt
    25321 atggaggtct cggtcgccgc ccagaatgcg cagctgacgg tcaacaacgt cgccatcgag
    25381 aacagcagca acactatcag cgacgcgctg gaaaacatca ccctgaacct gaacgatgtc
    25441 accacgggca accagacgct aaccatcact caggacacct ccaaagcgca aacggcgatt
    25501 aaagactggg tgaatgccta taactcgcta atagatacct tcagcagcct gaccaaatac
    25561 accgccgtag atgcgggagc tgatagccag agttctagca atggcgcact gctcggcgac
    25621 tccacgctgc ggacgattca gacgcagttg aagtcgatgc tgagtaatac cgtcagttct
    25681 tccagctata aaacgctggc gcagattggt atcacgaccg atcccagcga tggcaaactg
    25741 gaactggatg ccgacaaact caccgctgca ctgaaaaaag atgccagcgg cgtaggtgca
    25801 ttgattgttg gcgatggtaa aaaaaccggc atcacaacca ccatcggcag caacctgacc
    25861 agttggcttt cgacaacggg cattattaaa gccgctaccg atggcgttag taaaaccctg
    25921 aataagttaa ctaaagacta caacgccgcc agcgatcgca ttgatgcgca ggtcgcgcgc
    25981 tacaaagaac aatttaccca actggacgtt ttaatgacct cgttaaacag caccagcagc
    26041 tacttaacgc agcagttcga aaacaacagt aattccaagt aatcattatt catcgggaga
    26101 caggtcatgt acgcggcaaa aggcacccag gcctatgcac aaattggcgt cgaaagcacc
    26161 gtaatgagcg ccagccaaca gcagttggtc accatgctat ttgatggagt gctgagcgca
    26221 ctggttagag cgcgactgtt tatgcaggac aacaatcagc aaggcaaagg cgtctctttg
    26281 tcaaaagcga tcaacatcat tgagaacgga ctgcgggtga gtcttgatga agagagcaaa
    26341 gacgaattaa cccaaaactt aattgctctt tatagctata tggtcaggcg tttgctgcaa
    26401 gccaatttac gcaacgatgt ctccgcagtc gaagaggtgg aagcattaat gcgtaatatt
    26461 gccgatgtct ggaaagagtc tttactctcc ccttcttcga ttcaggaccc agtctgatga
    26521 acaatgcacc gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc cagctcatgt
    26581 tacgcctggc aacggaagaa caatgggacg aactcatcgc tagcgaaatg gcgtatgtga
    26641 atgcggtgca ggagatcgca cacctgactg aagaggttgc cccgtccact acgatgcagg
    26701 agcagctccg cccgatgctg cacctgattc tcgacaacga aagcaaggta aagcagttat
    26761 tacagattcg gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg caaaaatcgg
    26821 tgttaagtgc ctatggcgat cagggcggct ttgtgctggc tccgcaggat aacctctttt
    26881 gaatcttaat gagccgatgg ctcgcgaata atccgattac ggctacgctt ctaatattcc
    26941 ccttgaatgg agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc actggtatta
    27001 cccggaaggc ggtaagctct ggcctgaact ggccgagcgc gcagacggtt ttaatgatat
    27061 tggtatcaat atggtctggt tgccgcccgc ctataaaggc gcatcgggcg gctattcggt
    27121 cggctacgac tcctatgatt tatttgatct aggtgagttt gatcagaaag gcagcatccc
    27181 tactaaatat ggtgataaag cacaactgct ggccgccatt gacgccctga aacgtaatga
    27241 cattgcggtg ctgctggacg tggtagtcaa ccacaaaatg ggcgcggatg aaaaagaagc
    27301 tattcgcgtg cagcgtgtaa atgctgatga ccgtacgcaa attgacgaag aaatcattga
    27361 gtgtgaaggc tggacgcgtt acaccttccc cgctcgtgcc gggcaatact cgcagtttat
    27421 ctgggatttc aaatgtttta gcggtatcga ccatatcgaa caccctgatg aagatggcat
    27481 ttttaaaatt gttaacgact ataccggaga aggctggaac gatcaggttg atgatgaact
    27541 cggtaatttc gattatctga tgggtgagaa tatcgatttt cgcaatcatg ccgtgacgga
    27601 agagattaaa tactgggcgc gctgggtgat ggaacaaacg caatgcgacg gctttcgtct
    27661 tgatgcggtc aaacatattc cggcatggtt ttataaagag tggatcgaac acgtacagga
    27721 agttgcgcca aagccgctgt ttattgtggc tgaatactgg tcgcatgaag tcgataaact
    27781 gcaaacgtat attgatcagg tggaaggcaa aaccatgctg tttgatgcgc cgctgcagat
    27841 gaaattccat gaagcatcgc gcatgggtcg cgactacgac atgacgcaga tcttcactgg
    27901 gacgctggtg gaagccgatc cttttcacgc cgtaaccctc gttgccaacc acgacaccca
    27961 accgttgcaa gccctcgaag caccggtcga accgtggttt aaaccgctgg cgtatgcctt
    28021 aattttgttg cgggaaaatg gcgttccttc ggtgttctat ccggacctct acggtgcaca
    28081 ttacgaagat gtcggtggtg acgggcaaac ctatccgata gatatgccaa taatcgaaca
    28141 gcttgatgag ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga cattattttt
    28201 cgaccatccg aactgcattg cctttagccg cagtggcacc gacgaatatc ccggctgcgt
    28261 ggtggtcatg tcgaacggag atgatggcga aaaaaccatt catctgggag agaattacgg
    28321 caataaaacc tggcgtgatt ttctggggaa ccggcaagag agcgtagtga cagacgaaaa
    28381 cggcgaagcg accttctttt gcaacggcgg cagcgtcagc gtgtgggtta tcgaagaggt
    28441 gatttaaatc catccccggc ggcaagccgg ggagatttca ttacggcagt ggcgtcggta
    28501 acggcgcttt atccagcgcg gcggcacact ctgccgtcgg gcgatccaca cgctccatcg
    28561 tcataccatc gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc tcacgtttta
    28621 cggtaacgtt agtgagatca tcagacatca atgtcaactt acccggcacg gcaatccccc
    28681 gctgccactg acggcaatcc aaggtatcgc cctctttagt gacaatcagg ctgcctatcg
    28741 cttccggact gaccagcgca cgttgcggcc ctttggtctg ccagtagccc gccagccagt
    28801 ccggcgcggg ggttttcacg acattgttat agttttcgac ttcggcacag cccgccagca
    28861 acatgagcgc gcctgcaatt gcgagttttt tcatcattcg tcctgcatga gaagaaaaga
    28921 ggattgtggc attaaagctc tgatgtcgcc agcctttcca ggttcagaga gaaatttgat
    28981 ctaactatta tttttgtgaa ttattacccc gaaaataaat ttgcatggtg tatgattcgc
    29041 ggctcttgtt tcaaaccttc tgagttcaga ggctacattc atgtcatggc agcaattcaa
    29101 acacgcctgg ttgattaaat tctgggcacc catccctgcg gtcatcgcgg cgggtattct
    29161 ctccacttac tattttggca ttactggcac cttttgggct gtcacgggtg aatttacccg
    29221 ttggggcggt cagctcctgc agctgtttgg cgtccatgct gaagagtggg gttattttaa
    29281 aattatccat ctggaaggat cgccattaac ccgcatcgac ggcatgatga tcctcggtat
    29341 gtttggcggc tgctttgctg ctgcgctgtg ggccaacaat gtcaaactac gcatgccacg
    29401 cagccgtatc cgcattatgc aggccatcat tggcggcatt atcgccggtt ttggcgcgcg
    29461 tctggcaatg ggctgtaacc tggcggcgtt ctttaccggc attcctcagt tctcgctgca
    29521 tgcctggttc tttgccatcg ccactgccat tggttcatgg tttggcgcgc gctttaccct
    29581 gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct caccgctgac
    29641 gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct ttttcggcat
    29701 gctgggctgg gcgctgctca cggcgatgaa ccaaccaaaa ctggggctgg caatgctgtt
    29761 tggcgtcggc tttggtttac tgattgaacg tgcgcaaatc tgctttactt cggcgttccg
    29821 cgatatgtgg atcaccggac gtacccatat ggcgaaagca atcattatcg gtatggcggt
    29881 gagtgccatc gggatcttca gttacgtaca gttaggcgtt gaacctaaaa tcatgtgggc
    29941 gggaccaaat gcggtaattg gtggtttact ttttggtttt ggcatcgtgc tggcaggcgg
    30001 ctgcgaaacc ggctggatgt accgcgcggt agaaggccag gtgcactact ggtgggtcgg
    30061 tctgggcaac gtgatcggct caacgattct ggcgtattac tgggatgatt tcgctccggc
    30121 gctggccacc gactgggaca aaatcaacct gctgaaaact tttggtccga tgggaggcct
    30181 gctggtgaca tatttgctgc tgtttgctgc gctgatgttg attatcggct gggaaaaacg
    30241 cttcttcccc cgtgaggcac cgcagactgc taaggagatc gcatgaaaaa tatcgttcct
    30301 gattaccgcc ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc aacccttgag
    30361 gcgatgccgc aattaaaaaa aggtgagatc ctggaagtgg tgagcgactg tccgcagtcg
    30421 atcaataata ttccactgga tgcgcgtaat cacggctaca cggtgctgga tattcagcaa
    30481 gacgggccga ccattcgtta tttaattcag aagtaatccc tcattcccgc tgtggcccac
    30541 agcgggaaca tcgttacctc tgacacatag attttacccg ccccctctcc tattcttttc
    30601 agtatgatct caggaggttg ctatgtgtgg acgctttgcc caatcccaaa cgcgtgagga
    30661 ttaccttgcg cttctcgcgg aagatattga acgcgatatt ccctacgatc ccgaacccat
    30721 tggcagatac aatgtcgcgc cgggaaccaa agttcttctg ctcagtgaac gtgatgaaca
    30781 ccttcatctg gatccggttt tctggggata tgctcccgga tggtgggata aaccgccgct
    30841 gattaacgcc cgcgtagaaa ctgcctcaac cagtcgcatg tttaaaccgc tctggcaaca
    30901 tggtcgggca atctgttttg ccgatggctg gtttgagtgg aaaaaagaag gcgacaaaaa
    30961 aagccttatt ttatctatcg cgctgacgga caacctgttt ttatggccgc gataggtagc
    31021 ataccgtttg agcgtggtga cgaagccgaa ggatttttga tcgtcactgc ggcggcagat
    31081 caaggtctgg tagatattca tgaccgacgc ccgctggttc tgtcgccaga agctgcacgg
    31141 gaatggatgc ggcaagacat tggtggtaaa gaagcctcag aaatcgctac cagaagctgt
    31201 gtgccagcaa accagttcac ctggcaccct gtgtcgcgca cggtgggtaa tgtcaaaaac
    31261 cagggcgcgg agttaattca acctgtttgt tgacatcctc cacgccctga atgacgagga
    31321 cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
    31381 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
    31441 agctccattt acatcggcgt tcgctgtata tccacatacc tggcatctga atttactttg
    31501 tgacaggcga ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
    31561 tggcggaaca gcaagcacct gaccgccacg ccagagctgc ttgtactcaa gctggcggcg
    31621 catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt
    31681 gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc
    31741 ctcaatgact atcattacgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc
    31801 cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
    31861 gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca gcttcttctg
    31921 gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg aggcaagttt
    31981 agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag ttgatacttc
    32041 actttctgcc tgaatactga tgtaccactt accgcaggac tggctgacag tgacattttt
    32101 cacaacaccc gtgacctgac ggctgttccg gtagcgcatc cagccaagtt ttggcagaaa
    32161 aatacggctg ttttcctgag cgagcttaac gccctgcgga tagcggaatg catcattctg
    32221 tcaccgcttt ttgaatcagg ggaacgccga gcgcttctgg aaaaagtttt tgcaggcctg
    32281 ctcagggtct ttcagtgact gttgcaatgg ctgtgacgga gcatctttaa tccattgcgt
    32341 ttcagtggca tttttccact caaccaatca ggaagccatt ttcgcgtaag ggatgtcttt
    32401 gttaccggcc ttatgattct cattctgacg tgccagagta cgactgaaaa cgaaacgaca
    32461 tgcgcctgcg aagcgcctca tttcacgcgc ctgttgacca ccgggtctta accggaattt
    32521 gaatacctgt agtcgtttca cagggcaaat tatactttgg tctgtgaaaa aaactgatat
    32581 ccgacgcgga aggcactgtg ttttcctgat gcatgctcac cgggtctttg ttaccaagta
    32641 ccgacgccag atttttgacc atgacgcgac agaaaaacta cgcacttact tttcaaatgt
    32701 atgtgcttat tttgaagctg aactggttga aatggatggc gaaccagatc acgtccatct
    32761 gttaattaac tatcccccca aactggcgat atccagtctg gtaaacaaac agcctcaaag
    32821 gcgtatcggg taggttactg cgacgggatc gaccagatat tgctgccagg tattaccgca
    32881 aagatgtttt gtggcgtccc ggctattttg ccagtagctg cggaggtgcg ccaatatccg
    32941 tcatccgcca atacattgaa caacagcaaa cacctggtca gatgaaaaaa cgagccttat
    33001 atccccgccc tgaaggacgg gcttttacgg tgcactggat aagcctggag attatctaca
    33061 cctgcatgga catcattgta ctatgccccg tacatgcccc atcacatcct caacatcaaa
    33121 gtattagagc cttgcaaacc attatgcgtc tcagtttgtc tcatctatct tacgcaaaaa
    33181 aaatagaact aatatcaagc ggcgctatat tgcgtattaa ataaaaccac ctcaattaca
    33241 ttaataaatc taaataaata tcatgcaaat aatttccatg gttattttta ggctatttaa
    33301 attaaaaaaa taaattatga gtcaatcata aataacaacc tttaatataa tttgactacc
    33361 aaatttatat tcgttcacaa aatagtcatg cattgtgtgc aatagaaaca gttcagataa
    33421 aaatagggat ctacttcaca atttaaatga gaaactaaaa cttacatctt gaaataatct
    33481 cattgattgg atgaatattt gtagtgcagt gacatcattt ttaaataata gttcaaataa
    33541 aagggctcac gatgaaaaaa ttaacagtgg caatttctgc tgtagctgca tcagtactga
    33601 tggcgatgtc tgctcaggca gcagagattt acaataaaga cagtaacaag ctggatctgt
    33661 acggaaaagt taatgctaag cactacttct cttctaacga tgcagatgat ggcgacacaa
    33721 cttatgttcg tctgggcttc aaaggcgaaa ctcaaatcaa cgatcaactg actggtttcg
    33781 gtcagtggga atatgaattc aaaggcaacc gtgctgaatc tcaaggttct tccaaagata
    33841 aaacccgtct tgccttcgct ggcctgaaat tcggtgacta cggctccatc gattatggcc
    33901 gtaactacgg tgtagcatac gatatcggtg catggaccga cgttctgcca gaattcggtg
    33961 gcgatacctg gacccaaaca gatgtgttca tgactggtcg caccacaggt gttgcaacct
    34021 atcgtaacaa cgacttcttt ggtctggttg atggtctgaa ctttgctgct cagtatcagg
    34081 gtaaaaatga ccgcactgac gtaactgaag ctaacggtga tggtttcggt ttctccacta
    34141 cttatgagta tgaaggattc ggtgtaggtg caacctatgc taaatctgac cgcactaatg
    34201 atcaggttat ctacggtaac aacagcctga atgcatctgg tcaaaatgct gaagtatggg
    34261 cagctggtct gaaatatgat gcgaacaaca tctatctggc taccacctat tctgaaaccc
    34321 agaacatgac tgtttttggt aataaccata ttgccaacaa agcacaaaac ttcgaagtag
    34381 ttgcacaata tcagttcgac ttcggtctgc gtccgtccgt tgcttacctg caatctaaag
    34441 gaaaagactt gggtgcgtgg ggtgatcagg acctgattga atatattgat gtaggtgcaa
    34501 cctattactt caacaaaaat atgtccactt ttgttgatta caaaatcaac ctgattgata
    34561 agagcgattt cacgaaagca tctggcgttg ctaccgatga tatcgttgct gtaggattgg
    34621 tttaccagtt ctaatctgat tactcaaaga tatgttgtgg gaggctttgc ctccccaaca
    34681 tataagtggc gctctcaagc cactttcctt agaagcacta tcttgcttct gactatataa
    34741 attacccttt attttctggg acgtttcaac gccccatttt taataatact cagtgaacaa
    34801 ttaacgtatc aattagattt aataacaacg atatccgtac ctaacctgat atctaatgtc
    34861 ataagcatcc ctttaattat cccctcaacc tcttgtaaat ttcccactct tgagctccac
    34921 atagcccaaa cgccggatgc aaaaaaaatc acacgtgcat ctctgaaatt gtgatggagg
    34981 aagtgagtat caattatatg tgcttataaa cgttcccgat tggcacagaa gtcaccggat
    35041 tgttcaggct ccaataggtt gagtatgtcg tatttatttt ccaaaataaa aatcgatgct
    35101 gttgctcctt aataaaatac ttttactctc tggacgcttt tcttagctct cttggtgtta
    35161 tattaaaacg attatggaac ctttcagtaa aacgagaagg acacttgtaa ccattttccc
    35221 tggcaatctc gctgatgggt tttactgtcg tttgtatagc aggacaacgc attatttaac
    35281 ctcacgtcgt ccaatatact ttgaaaactt attccctcgc ttgccagacg gcgatgcaat
    35341 gtggaaacag aaatgtagag atatctcgct accttgtttg ctgtccattt tgttctaggt
    35401 tcggatagca ttaggttata acaacgactt attaatgatt gtttactatc tgataaaagt
    35461 aaacaattaa catgatgtgc tcctaatgaa agtagaacgc ccattgctaa gtgttcctga
    35521 atttgcgttg agaagcctcg ggaaatagat gtttttagtt gctcccaaca atatattaac
    35581 tcggggttct gaggtaagca gaaacctgtc gtgttacgaa tttgatcagt tatcgcataa
    35641 aatttttgaa acttttcaat tagatcaatg ggataataaa gcatttctgc aagataaaga
    35701 cctgcttctg gataattcac aatataaaat tcatatccac agggaaataa tattatctga
    35761 ttattatcaa cagttagact atgcgtctcc caattgataa ctttttttcc ctgacggata
    35821 cgacataaag ctggcataag aggcttaacc ctgtgaatct catgatgttt atgcatccgt
    35881 atttcttcaa tctttaggtt agacttatct cttgccagca tactcttacc ctattttatc
    35941 tcataaactg gtgttatctc agcggttgca attttattgg cattaagcat ataaccaact
    36001 aacgctccgc tggaattaga atccacagga atcttatcag tttttagagc ccatacttta
    36061 aactggtaat gatggggttt atctcctttg ggaggacatg ctccaccaaa tcctgcataa
    36121 ccaaaatcat ttcggccttg aacagcacca gtcggaagtt ttgtaccatc acgtcttcct
    36181 gcatcagtgg gcaaatatgt tacagttgct ggaatattag caacagtcca gtgccaccat
    36241 ccactgcctg taggagcatc tggatcatac acagttacgg caaagctttt ggtgccttca
    36301 ggagcaccag accaggttaa tgagggcgat gtattaccac cttcacaccc aaatccagaa
    36361 aatacatgag acgttgtaag ttgctctcct gtttttattt cattactagt gacctgaaat
    36421 gctgcggcct gcgcagaaaa tgttatgaat gccaatacac ttgaaacgat aagttttttc
    36481 ataaaaacct cattgttgtg acctatcgtt attttatttg atgtgccttg atctcattat
    36541 gaataaaggc gcaatgatcg cgcaaatgca atcataatta taaaggtgcc ccgaacatta
    36601 gccataatat gcacaaatca gattcctgtt atctcaagga gaattatttt ttaatgcggt
    36661 gcacttcgtg acaacaagtt aatgactaac acaccggcac aaatcaacat catgcctatg
    36721 atagctggca ggtccagtcg ttgaccaaaa aatccccagg aaagcaaact aatcagaacg
    36781 ataccgactc ctgaccagat agcataagca atccctgtag gtatataagc cagcgtctga
    36841 gctaataacc agaatgatgc acaatagcaa ataattgtgc caacagaggg ccataaccgt
    36901 gtaaacccct ctgaaaactt cattaaggtt gtaccaatga cctctgcaag tattgcgcca
    36961 ccaagataaa tataagggtt catagcatat tctttcctgt tcatactgga gagaattgta
    37021 ctacagtttg aacacaactc accgttttca tcctggtgta tgtgtatcca aggggctctg
    37081 gcgtggcaag agataaaggc aggaacaata atgatcagaa ggaaccccag gctaacgaat
    37141 gagttcactc ttcctgggag caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt
    37201 ggatggcaga aaacaaccag gatcaggtat ccattttaaa ccagaaattg agtgagtttg
    37261 ccccatccat gccccacgct gtgggatcgg atataattaa acaagcatag tcaacaggtt
    37321 acacaaccta cacctgcatg gacatcactt cctgatacgc cgccaccagc ttattacgca
    37381 cctgaatccc catttgcata gaaactgagg ctttttgcat atcggtcatc acatcgttta
    37441 gcgccacgcc gggttcaccg agggtgaatt tttctgcctg cgtgcgggcg actgtttgtg
    37501 tatcgcttat gcgatcgagc gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg
    37561 gcagtgattc ctgcgcacac gcactcatcg ccgtagcctg taactggctg ataacccctt
    37621 caatcccctg tatcgctgac attttcgtct cccggataat ttctggtaac aaagcctacc
    37681 agtaagtcaa taagacaaag gcgctaaata gcaacaaaaa aactggttta ttggcggata
    37741 gaaaaaacga aagcacaaat aatgggagcg tcaatttttc gagtttgctg acccgagagt
    37801 gagtcttgtt ccactttgcc aataacgccg tccataatca gccacgaggt gcgcgatgaa
    37861 tgcgactgca gcccagacaa aatctcttga gtggcttaat cgcctgcgtg cgaatccgaa
    37921 aattccattg attgttaccg gttccgcggc agtggcggtc atggtcgcac tgatcctgtg
    37981 ggcgaaagcc cccgactacc gcacattatt cagcaatctt tccgatcagg atggtggcgc
    38041 aattgtcagc caactgacgc aaatgaatat tccttaccgc ttcagcgaag ccagcggcgc
    38101 tattgaggtt ccggcagata aagttcacga actgcgcctg cgcctggcgc aacaaggttt
    38161 gcccaaaggc ggcgcggtcg gtttcgaact gctcgatcag gaaaagtttg gtatcagcca
    38221 gttcagcgaa caggtgaatt atcagcgggc gctggaaggc gagctttctc gtaccatcga
    38281 aactatcggc ccggtaaaag gggcgcgcgt acatctggca atgccgaaac cgtctttatt
    38341 cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta aatctgttac ccggccgcgc
    38401 actcgatgaa gggcaaatta gcgctattgt acatctggtt tccagcgcgg ttgctggtct
    38461 gccgcctgga aacgtcacgc tggtggatca gggcgggcat ctgttaaccc agtccaatac
    38521 cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc agcgatgtcg aaggccgtat
    38581 tcagcagcgt attgaagcga tcctgtcacc tattgttggt aacggtaata ttcacgccta
    38641 ggtcacggcg caactggact tcgccagtaa agaacaaacg gaagaacagt atcgccctaa
    38701 cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt aatgagagcg agcaaagcgg
    38761 ttccggttat ccgggcggcg taccgggggc gttgtcgaat caaccggcac ctgcgaataa
    38821 cgcgccaatc agcacgcctc cgacaaatca aaataaccgc cagcagcagg cgagcaccac
    38881 cagcaatagt gggccgcgta gcacacagcg gaatgaaacc agtaactacg aagtcgatcg
    38941 caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt
    39001 ggtgaattac aaaaccttgc cagacggtaa accattgcct ctcagcaacg aacagatgaa
    39061 gcaaattgaa gctctgaccc gcgaggcgat gggcttttct gaaaaacgcg gtgattcgct
    39121 caatgttgtt aactcgccgt tcaatagcag tgacgaaagc ggcggagcac tgccgttttg
    39181 gcaacagcaa gtgtttatcg atcagttgct ggctgccggt cgctggttgc tggttgctgg
    39241 tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta acacgtcgcg
    39301 ccgaggcggt gaagactgtg cagcaacagg cgcaggccca cgaggaagtg gaagatgcgg
    39361 tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac caacgactgg
    39421 gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg
    39481 cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg caccgataaa
    39541 agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt caagcacctc
    39601 tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca gatctccaac
    39661 aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt tgctgcactg
    39721 aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg tgaagaacgt
    39781 gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg tattgaaacg
    39841 ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca tccgcaaatt
    39901 atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct ggcgttgttc
    39961 gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg cgtgcagcca
    40021 gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca gaatctcaag
    40081 cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat gaaaacgcag
    40141 caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc gcagaaaatc
    40201 atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag cattcagcgt
    40261 ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc cgagcagcca
    40321 ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct gcgcgacgat
    40381 ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa agcgattctg
    40441 ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg cgaggatacc
    40501 tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc caccaccggc
    40561 agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg aacccagcct
    40621 tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc aggcggggat
    40681 tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg cccaggggct
    40741 ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc ggatgcagca
    40801 actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag cttcgcgcct
    40861 gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa cggtggataa
    40921 ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat tcagcgggaa
    40981 accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc tcggcgcaac
    41041 tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg gcggatgtaa
    41101 agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc aagaactctg
    41161 ccgtctggca gcaccaggag tggtgtaatg accacgcgcc tgactcgctg gctaaccacg
    41221 ctggataact ttgaagccaa aatggcgcag ttgcctgcgg tacgtcgcta cgggcgatta
    41281 acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct cggcgcaacc
    41341 tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga agtcgttggc
    41401 tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt cctgcccggc
    41461 gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa gcagttgccg
    41521 ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct cgatggcctg
    41581 cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa cccgttgcaa
    41641 cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc
    41701 gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa aagtgtgctg
    41761 ctggggatga tggcacgcta cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa
    41821 cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg acgtgcacgc
    41881 tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca aggtgccgcc
    41941 tatgccacgc gcattgccga agattttcgc gatcgtggtc agcatgtgtt gctgattatg
    42001 gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat tggcgaacca
    42061 cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact ggtcgagcgt
    42121 gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt gctcaccgaa
    42181 ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga cggtcacatt
    42241 gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat tgaagcgtcg
    42301 atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc
    42361 aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc
    42421 aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct ggagggctat
    42481 ttgcaacaag gcatttttga acgcgcggac tggaaagcgt ctctccaggg gctggagcgt
    42541 attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaga acagctcaaa
    42601 atgttgatcg attatcagaa tgaatatcgc aataacctca atagcgatat gagtgccggg
    42661 atgaccagca accgctggat caactatcag cagtttatcc agacgctgga aaaagccatt
    42721 actcagcatc gccagcaact taatcagtgg acgcagaaag ttgacattgc cctgaacagc
    42781 tggcgagaaa agaaacaacg tttgcaggcc tggcagacac tgcaggaacg gcaatccacg
    42841 gcggcactgc ttgcagaaaa ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc
    42901 gccgccatga ggaaacctga atgattcgct tagcgccctt gattaccgcc gacgttgaca
    42961 ccaccacatt gcctggcggc aaagccagcg atgctgcaca agattttctc acgttgttga
    43021 gcgaagcatt agcaggcgag acaacaactg acaaagcggc cccccagttg ctggtagcaa
    43081 cagataagcc cacgacaaaa ggcgagccgc tggtcagcga tattcttgcc gatgcgcaac
    43141 aagcggattt actgatccct gtggatgaaa cacttcctgt catcaacgac gaacaatcca
    43201 catcaacacc gttaaccacc gctcagacga tgacgttggc tgcggtggct gacaaaaata
    43261 cgacaaaaga cgaaaaagcg gatgatctta atgaagacgt caccgcaagt ctgagcgccc
    43321 tttttgcgat gttgccgggt tttgacaata cgcccaaagt gactgatgca ccgtcaaccg
    43381 tgttaccggc agagaaacca acgctcttca caaaactgac ttctgcgcaa ctcactacag
    43441 cacagcctga tgacgccccc ggcacaccag ctcagccatt aacaccgctg gtagcagaag
    43501 cccagagtaa agcggaagtc atcagcacgc cttcgccggt gaccgctgac gccagcccgc
    43561 taatcactcc acaccagaca cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac
    43621 cgctgggttc tcacgaatgg caacaatcat tgagccagca tatttcgctg ttcacccgcc
    43681 aggggcaaca aagtgcagag ttgcgtctgc acccacagga tttaggtgaa gtgcaaattt
    43741 ccctcaaagt ggatgataac caggcgcaga tccagatgat ttcaccgcat cagcacgtac
    43801 gcgccgccct ggaagcagcg ctgccggtac tgcgtacgca gctggccgaa agtggcattc
    43861 agttagggca aagcaacatt agtggcgaaa gctttagtgg tcagcagcag gccgcttccc
    43921 agcaacagca aagccaacgc acagcaaacc atgaacctct ggcgggggaa gacgacgata
    43981 cgcttccggt tcccgtctct ttacaagggc gtgtaacagg taacagcggc gttgatattt
    44041 tcgcctaacg tcagaggtag caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa
    44101 gacgcaggat aattagccga taagcagtag cgacacagga agaccgcaac acatgactga
    44161 ttacgcgata agcaagaaaa gcaagcgatc gctttggatc ccgattctgg tattcattac
    44221 cctcgcggcc tgtgccagcg caggttacag ctactggcat tcgcatcagg ttgccgctga
    44281 cgacaaagcg cagcaacgcg tcgtgccctc cccggtcttc tatgcgctgg atacctttac
    44341 ggtcaatttg ggcgatgcgg atcgcgtgct ttatatcggc ataaccctgc gactgaaaga
    44401 tgaagcaacc cgctcgcggc tgagtgagta tttgccggaa gtccgtagtc gcttgctgtt
    44461 actgttttca cgtcaggatg ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat
    44521 tgccgagatt aaaaccacac tttccacccc gcttgttgcc gggcaaccga aacaggatgt
    44581 caccgacgtg ctgtataccg cttttattct gcgataacga catgggcgat agtattcttt
    44641 ctcaagctga aattgatgcg ctgttgaatg gtgacagcga agtcaaagac gaaccgacag
    44701 ccagtgttag cggcgaaagt gacattcgtc cgtacgatcc gaatacccaa cgacgggttg
    44761 tgcgcgaacg tttgcaggcg ctggaaatca ttaatgagcg ctttgcccgc cattttcgta
    44821 tggggctgtt caacctgctg cgccgtagcc cggacattac cgtcggggcc atccgcattc
    44881 agccgtacca tgaatttgcc cgcaacctgc cggtgccgac caacctgaac ctaatccacc
    44941 tgaaaccgct acgcggcaca gggctggtag tgttctcgcc gagtctggtg tttatcgccg
    45001 tggataacct gtttggcggc gatggacgct tcccgaccaa agtggaaggt cgcgagttta
    45061 cccatactga acagcgcgtc atcaaccgta tgttgaagct ggcgctggaa ggctatagcg
    45121 acgcctggaa agcgattaat ccgctggaag ttgagtacgt gcgttcggaa atgcaggtgg
    45181 aatttaccaa tatcaccacc tcgccgaacg acattgtggt taacacgccg ttccatgtgg
    45241 agattggcaa cctgaccggc gaatttaata tctgcctgcc attcagcatg atcgagccgc
    45301 tacgggaatt gttggttaac ccgccgctgg aaaactcgcg taatgaagat cagaactggc
    45361 gcgataacct ggtgcgccag gtgcagcatt cacagctgga actggtggcc aactttgccg
    45421 atatctcgct acgcctgtcg cagattttaa aactgaaacc cggcgacgtc ctgccgatag
    45481 aaaaacccga tcgcatcatc gcccatgttg acggcgtccc ggttctgacc agtcagtacg
    45541 gtactctcaa cggtcagtat gcgttacgga tagaacattt gattaacccg attttaaatt
    45601 ctctgaacga ggaacagccc aaatgagtga catgaataat ccggccgatg acaacaacgg
    45661 cgcaatggac gatctgtggg ctgaagcgtt gagcgagcaa aaatcaacca gcgaaaaaag
    45721 cgctgccgac gcggtgttcc agcaatttgg cggtggtgat gtcagcggaa cgttgcagga
    45781 tatcgacctg attatggata ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat
    45841 gaccatcaaa gagctgttgc gtctgacgca agggtcagtc gtggcgctgg acggtctggc
    45901 gggcgaaccg ctggatattc tgatcaacgg ttatttaatc gcccagggcg aagtggtggt
    45961 tgttgccgat aaatatggcg tgcggatcac cgatatcatt accccgtccg agcggatgcg
    46021 ccgcctgagc cgttagtgat gaataaccat gctactgtgc aatcttccgc gccggtttct
    46081 gctgagccac tgctgcaggt gagcggcgca ctcatcgcca ttattgccct gatcctcgct
    46141 gctgcctggc tggtaaaacg gctgggattt gcccctaaac gcactggcgt taacggtctg
    46201 aaaattagcg ccagtgcttc gctgggcgcg cgtgaacggg tcgtggtggt cgatgtggaa
    46261 gatgcacggc tggtgctcgg cgttaccgca gggcaaatca atctgctgca taaacttccc
    46321 ccttcagcac caacggaaga gataccgcag accgattttc agtcggtcat gaaaaatttg
    46381 cttaagcgtg gcgggagatc ctgatgcgtc gtttgttttc tgtcgcaccg gtccttctct
    46441 ggctgattac gcccctcgcc ttcgcgcaac tgccgggtat caccagccag ccgctacctg
    46501 gcggtggaca aagctggtcg ctcccggtgc agacgctggt gttcatcacc tcgttgacgt
    46561 tcattccagc gattttactg atgatgacca gtttcacccg catcattatt gtttttggtt
    46621 tattgcgtaa cgcgctggga acgccctccg cgccacctaa ccaggtattg ctggggctgg
    46681 cactgttttt gacctttttt attatgtcgc cagtgatcaa caaaatttat gtcgatgcgt
    46741 accagccatt cagcgaagag aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc
    46801 cgctgcgtga gtttatgctg cgtcagaccc gcgaggcaga tttagggttg tttgccagac
    46861 tggcgaatac cggcccgttg caggggcctg aagctgtgcc gatgcgcatt ttgctcccgg
    46921 cctacgtgac cagcgagttg aaaaccgcat ttcagatagg cttcacgatt ttcatccctt
    46981 ttttgattat cgacctggtg attgccagcg tgttgatggc attggggatg atgatggttc
    47041 cccccgccac cattgctctg ccctttaaac tgatgctgtt tgtactggtg gatggctggc
    47101 aattgctggt cggttcgctg gcgcagagct tttacagcta gagaggcaaa atgacacctg
    47161 aatcggtcat gatgatggga actgaagcga tgaaagtcgc gctggcactg gcagccccgc
    47221 tgttgctggt agcgctggtc acgggcctta tcatcagtat tttgcaggca gccacgcaga
    47281 ttaacgaaat gacgctgtcg tttattccga aaatcatcgc cgtatttatc gccatcatta
    47341 ttgccggacc gtggatgctc aatctgttgc tggattacgt ccgcaccttg ttcactaacc
    47401 tgccgtatat catcgggtag cccgtacaat gatgcaggag acaagcgatc aatggctttc
    47461 ctggttaagc ctgtacttct ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc
    47521 aattctgagc gaacgcagcg tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac
    47581 gttcgccatt gccccatcat tacctgccaa cgatgttcct gttttttcgt tctttgctct
    47641 gtggctggcc gtacagcaga tcctgatcgg cattgcgctt ggttttacca tgcaatttgc
    47701 ctttgccgct gtgcgaaccg ctggcgaaat tatcggtctg caaatggggc tgtcatttgc
    47761 gacatttgtc gatcctggca gccatctcaa tatgcccgtt ttagcgcgca tcatggatat
    47821 gctggcatta ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt
    47881 cgataccttt cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc
    47941 acccaccaaa gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat
    48001 tactctgttg ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt
    48061 atccattttt gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc
    48121 attaatgccg ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct
    48181 ggctgatatt attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct
    48241 aaggattatc cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata
    48301 tttgttttaa gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta
    48361 tacaacactt tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg
    48421 acccatgttg ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc
    48481 attgagtgag ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta
    48541 ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa
    48601 accgttgatg accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat
    48661 gaggactgtt tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa
    48721 catcccaata cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat
    48781 ctattggtca gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac
    48841 gatatccttg gcaatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg
    48901 acgttatcat tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga
    48961 accattcaaa tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt
    49021 aatattaaac gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg
    49081 acggataatg tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtgat
    49141 ttcccaccag tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat
    49201 gccctgcttc attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca
    49261 ttgtgccttc actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat
    49321 ccgttttgac tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca
    49381 gtgtagaaca atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga
    49441 cggtctatac ttaagagatg ctaggcggac ttaacgactg gcggcaacaa cagagtaacg
    49501 gttgcgagga aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt
    49561 cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca
    49621 tcgcagccag aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg
    49681 tgaattggcg gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg
    49741 ttgaattcac aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca
    49801 agaagcactt aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca
    49861 atttttttcg ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag
    49921 aaatgtgacg cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa
    49981 tcatctttga ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac
    50041 catgttttca attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga
    50101 cagtcatagt tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa
    50161 tattcccgtc attctctgta gcagtaaaac tttagcggaa atgctgtact tgcaaaaaat
    50221 gttggggcta caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga
    50281 acaatggcag gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat
    50341 ctgccaggtt ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga
    50401 tgtcgacgat gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac
    50461 gcagctgcat gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca
    50521 atttatcgct cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca
    50581 cgtactggat gcctctgccg gaaaagatca ggctgccaat tggattatcg cgacctatca
    50641 acaattgtca ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc
    50701 tttactggag gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca
    50761 tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg
    50821 gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg
    50881 attgcgcccg gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc
    50941 aaaatcataa tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat
    51001 cgttgtactc ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc
    51061 gacctccgca gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc
    51121 gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga
    51181 aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag
    51241 atcgacctga atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg
    51301 ggctttttcg aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg
    51361 ccactgcaac gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca
    51421 ggagaggagt aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact
    51481 gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa
    51541 atggtccaga cgttcccagc taacatagcg actgtccata cgaatgccgc cccgcgtgtc
    51601 atgttccatt gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg
    51661 atggtcagga ttggaagagg tcaaaaatct cagcttactg tcatagagct gatactcacc
    51721 atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact
    51781 catccccagc acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg
    51841 cggttcagta ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg
    51901 attttctcgt tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt
    51961 tggtacattg cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac
    52021 atacatcgcc tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac
    52081 ttccagtgca gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc
    52141 ctcgctgact aatgcatccg atacgccatt gactggcagg gtgcgccgtc ggttgagttc
    52201 gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga
    52261 ggtgaaatcc agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt
    52321 gtcgacgtta tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg
    52381 ctagctggag atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga
    52441 aaaaagcaat accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc
    52501 gagttttttc aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc
    52561 caacctgctt ttttatgatt ctggcataac ccggcaatag cgtaccgcaa aacagcgata
    52621 attttataat ggtggcatga taagacgcga ctatcattca ttgcaggcat tgggacacca
    52681 ggccggatgt gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga
    52741 ctcccctccc ccggaacaag atcggtttca tgaaaagctt cccgcttgac cccgtaaccg
    52801 tcataccaac gacactcaac cataccgctg gagtatccag tgacaatcat ccgcgggccg
    52861 ccctctttaa ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga
    52921 gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc ataatagcca gtgcgagtat
    52981 taatgtgcct gaccgcgcat tttcgctact gctttcactc ccagcaccac gatgccgccg
    53041 atgataaatc caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt
    53101 tgctgcccgg cgatatgttc aatagcgtga tgcagcggcg caataccatg taccacaatc
    53161 ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg ataatgcttt catcagccaa
    53221 ggcgcgataa tcaataaccc tttacctaat gcctgcatca gcgcgctaga tttttccgcc
    53281 agccaatacc ctaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg
    53341 gtcactacca gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg cgcctctgcc
    53401 acaatcccca gcgtgatggc gacgatttcc gcagacaaaa taaaatcggt acgaatcgcc
    53461 cctttaattt tgtccttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc
    53521 tgctggctct gcgctggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc
    53581 tctacccctt caaagcagag aaacgcgcca ccaatcatca acagtggcgt aatcgcccac
    53641 gggataaacg cactgatgat cagcgccagc ggcaccagaa tcactttatt gatcagcgat
    53701 cctttcgcca cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt
    53761 tgcgcattga gcgataaatc atcccctaat acaccggcgg ttttcttcgc cgccagtttg
    53821 cccatcacag agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt cagtaaacta
    53881 ctcccggcca aaagcattcc ctctcatttt tattgttgaa gggtgtaagt atgaagcaaa
    53941 aacggcagct ctgatacttg cttcacaggt caacatttat ttaacaatca agtgcaatta
    54001 tatctctcgc cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccgccg
    54061 tgagggatta tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat
    54121 atcatttggg gctcaaccta ttttgtcatt cggattggcg tgggaagctg gcctccgtta
    54181 atgatggcgg gcgttcgatt ccaggcagcc ggtattttat tgctggcatt tttgctactg
    54241 cgcggacaca aacttccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta
    54301 ttgctggctg tcggtaatgg catggtgacg gttgccgaac atcaaaatgt tccttccggc
    54361 atcgccgccg tagtgattgc aaccgtgccc ctctttaccc tgtgcttcag tcgcctgttt
    54421 ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca ttgggcttgc cggaatcatc
    54481 atgctcaata gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc
    54541 ggctcgatta gctgggcgtt tggctcagtt tatggctcgc gcattacctt acctgtaggg
    54601 atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat cgcatcgatg
    54661 attgcgggtg aaaaactgac ggcgctccct tccctgtcag gcttccttgc ggtcggctat
    54721 ctggcgctgt ttggttcgat tatcgccatt aacgcttata tgtatttaat ccgtaatgtc
    54781 agtccggctc tcgccaccag ctacgcctac gttaacccgg tggtcgcggt cttgctgggt
    54841 acgggactgg gtggcgaaac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtt
    54901 ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc cggccaaacc cgttgtcgcg
    54961 ccagttattc aggatgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct
    55021 ggcaccttcg ccgcagatcc actcttccag acgctcggta agcgccgcat ccgtcagctt
    55081 ctcgcgccta cgtaacgcac actcccagac aatcagtacg cgccagccga gctcctgcaa
    55141 gcgactgata tcgcggcgat cgcgttcaac atttttacct atcttctcca gccagaactc
    55201 agttcgagtc gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccgtg
    55261 ggtaaatatc acgcagcgat attcatcaac gacaaaatcc gggcgtccgg gcagactggc
    55321 gtcctgaacg cgaaacgcaa ggccctgccc ggttaacaaa ctggcgaggc gcttctcaat
    55381 cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg cgggtggcct tatcgtgaac
    55441 gtcgaccatg ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa
    55501 gcagttttgc caccgcggca aagaccggca cgaccaccga gttgccgaac tggcgatagg
    55561 cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta
    55621 agcgcgcgca ttcacgaggc gttaaccgac gtggacgatg ttgctgattc agcggatcgt
    55681 caaagtcttt ctcacccgtg gccatatccc aaccgcgatc gattaaaatt tccgcgccat
    55741 ctttgtaata acgagcagac agcgtacggg tgacgctttg cggattgttc ggataaacca
    55801 ttccataacc gaagccgtta ccgcgcgcct gatgtttttt cgcatatcga tagaggtact
    55861 tccacagcac cggcgtcagg atatatttcg cctcaaccat cgggtccagc agctgcgcca
    55921 gcgtcactcg ctgtgcaggg aaacattcgc tgatatcacg cagagtaaaa tcggctttca
    55981 gattcagatc gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcaggaaat
    56041 gtttaccatc gatgattttc ggatcgtccg gcccgttatc ttctgcatcg gcaacgtcat
    56101 agcccagttc gtccagcgtc tgcatgatga tgcggaacgt ttttccctta tcgtgactct
    56161 tcaggttttt gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca
    56221 ccacatcaaa gaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc cgcccgagcg
    56281 agttcttttt cgatacgcca gccagcgaaa atggctgaca agggaaaccg gccagtaaaa
    56341 catcgtgttc aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt
    56401 cttgatgact gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccgggtcgc
    56461 aataatggtt ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc
    56521 actgtccacc aatcgattca aaaccacgac ggatgccgcc aattccggca aatagatcga
    56581 taaagcgaaa cgcataatgc ggatgatgtt ccggtggttt gggtaacaac gtttgcagat
    56641 gggcaaactc tttctcactt aaacggtgcc atgccgagtc attcgccagc gcacgtttta
    56701 aaattgccgc gctccagtga ttctcaccca caccattaag ctgcgccacc agcattttaa
    56761 catcataaat ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg
    56821 aatcggttac tgatatattt tcctgcatag atttaaccgg ccatctgaga tggccgacag
    56881 gttaacataa ttactgccgt ttagggccac gcgctcagcc agcgggtgaa aacgcatcta
    56941 tcaccttgtg atcgatacct tcattctccc ccgccagttc ggcaccgagc ttcgccatca
    57001 aacagcgcca agctcaatgc ccctgaaacg gcaaatacgc gcgccaggcc aatcgccccc
    57061 agggcttcca gccgatccgc atcctggacg attttagctt ccgttgttaa gggggcgatt
    57121 tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg cctcaatttt ctccgccgga
    57181 aattgcacga actcttcacg gagcaggcga cgcgtctctt ctgccgccag gattgaagaa
    57241 cgctgccgtt gcggatgatt tttcgccagg ctcacgatat cgtgaaaata acaggcggtt
    57301 aaaatcacca gcatatcgac atcgtcatct gccgccagtt tctgtgccgt agcccagaca
    57361 cggcgaaaat gacacaggtc atgcgccgcg tcctgatgtt gatgatggtt tttcaaccag
    57421 ttctcgaact gtgcctgcca gtgttgtaac tccatacgcg ctccgttgcc aaagagtcgc
    57481 aaccttagca gtttttattc atagcgtctg caacgtgcgg gacgaggtcg ggtgtaccat
    57541 gcaatgccgc ctaacaaggc accaaccagg gccataaaca gaaatccgac caatgctcct
    57601 aaccacttcc ccgcggtaga tcctaaatca ctttcaaatc ccgacacggg ggcatcgggg
    57661 atccggctca tcacccagat gtatcgcgcc attgcccaga cgaaatagcc acaccatgta
    57721 tagaaagccc acaatgaatc gccacggata atctagacac ttccgagccg ttgataatac
    57781 tggttttcat attctgtcgg tgacatctgt tcgctagaac catgccgacg cttactgtta
    57841 taaaacattt cgatgtaatc aaaaatatca ctgcgggctt cttcccgcgt tccgtagatc
    57901 tttttcttta tccgttcacg tttcaacaac tggaaaaaac tttctgcaac cgcattatca
    57961 tggcagttac cgcgacggct catgctaccc tccaggccgt gtgatttcag gaacgactgc
    58021 cactcatggc ttgtgtactg actgccctga tccgaatgaa ccagcacctg tttttcggga
    58081 ttacgcc
//
LOCUS       DAEEDS010000016        58048 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEEDS010000016 DAEEDS010000000
VERSION     DAEEDS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58048
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.016"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(24..278)
                     /locus_tag="KLH32_001045"
     CDS             complement(24..278)
                     /locus_tag="KLH32_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBE0137966.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="KLH32_001046"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="KLH32_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBE0137967.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="KLH32_001047"
     CDS             1228..2694
                     /locus_tag="KLH32_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE0137968.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="KLH32_001048"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="KLH32_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBE0137969.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="KLH32_001049"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="KLH32_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBE0137970.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="KLH32_001050"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="KLH32_001050"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBE0137971.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="KLH32_001051"
     CDS             5709..7265
                     /locus_tag="KLH32_001051"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBE0137972.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="KLH32_001052"
     CDS             7347..9161
                     /locus_tag="KLH32_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0137973.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="KLH32_001053"
     CDS             9162..10256
                     /locus_tag="KLH32_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBE0137974.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="KLH32_001054"
     CDS             10256..11281
                     /locus_tag="KLH32_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0137975.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="KLH32_001055"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="KLH32_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBE0137976.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="KLH32_001056"
     CDS             complement(12876..13220)
                     /locus_tag="KLH32_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0137977.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KLH32_001057"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KLH32_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBE0137978.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KLH32_001058"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KLH32_001058"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBE0137979.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="KLH32_001059"
     CDS             15616..17376
                     /locus_tag="KLH32_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBE0137980.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="KLH32_001060"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="KLH32_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBE0137981.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KLH32_001061"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KLH32_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBE0137982.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="KLH32_001062"
     CDS             19113..19340
                     /locus_tag="KLH32_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBE0137983.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="KLH32_001063"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="KLH32_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBE0137984.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="KLH32_001064"
     tRNA            21195..21271
                     /locus_tag="KLH32_001064"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KLH32_001065"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KLH32_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBE0137985.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="KLH32_001066"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="KLH32_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBE0137986.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KLH32_001067"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KLH32_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBE0137987.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KLH32_001068"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KLH32_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBE0137988.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KLH32_001069"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KLH32_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBE0137989.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KLH32_001070"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KLH32_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBE0137990.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KLH32_001071"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KLH32_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBE0137991.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KLH32_001072"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KLH32_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBE0137992.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KLH32_001073"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KLH32_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBE0137993.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KLH32_001074"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KLH32_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBE0137994.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KLH32_001075"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KLH32_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBE0137995.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KLH32_001076"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KLH32_001076"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBE0137996.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KLH32_001077"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KLH32_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBE0137997.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KLH32_001078"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KLH32_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBE0137998.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KLH32_001079"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KLH32_001079"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBE0137999.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KLH32_001080"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KLH32_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBE0138000.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KLH32_001081"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KLH32_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBE0138001.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="KLH32_001082"
     CDS             37391..37555
                     /locus_tag="KLH32_001082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138002.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="KLH32_001083"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="KLH32_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBE0138003.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="KLH32_001084"
     CDS             complement(38328..39347)
                     /locus_tag="KLH32_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBE0138004.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="KLH32_001085"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="KLH32_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="KLH32_001086"
     CDS             complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="KLH32_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBE0138005.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="KLH32_001087"
     CDS             complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="KLH32_001087"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBE0138006.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42527..43591)
                     /gene="ada"
                     /locus_tag="KLH32_001088"
     CDS             complement(42527..43591)
                     /gene="ada"
                     /locus_tag="KLH32_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBE0138007.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="KLH32_001089"
     CDS             complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="KLH32_001089"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBE0138008.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="KLH32_001090"
     CDS             complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="KLH32_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE0138009.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46691..49363
                     /gene="rcsD"
                     /locus_tag="KLH32_001091"
     CDS             46691..49363
                     /gene="rcsD"
                     /locus_tag="KLH32_001091"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBE0138010.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49380..50030
                     /gene="rcsB"
                     /locus_tag="KLH32_001092"
     CDS             49380..50030
                     /gene="rcsB"
                     /locus_tag="KLH32_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBE0138011.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="KLH32_001093"
     CDS             complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="KLH32_001093"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBE0138012.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53354..54130)
                     /locus_tag="KLH32_001094"
     CDS             complement(53354..54130)
                     /locus_tag="KLH32_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBE0138013.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54135..55784)
                     /locus_tag="KLH32_001095"
     CDS             complement(54135..55784)
                     /locus_tag="KLH32_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBE0138014.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55785..56344)
                     /locus_tag="KLH32_001096"
                     /pseudo
     CDS             complement(55785..56344)
                     /locus_tag="KLH32_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56503..>58048)
                     /locus_tag="KLH32_001097"
     CDS             complement(56503..>58048)
                     /locus_tag="KLH32_001097"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBE0138015.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14199 a        15658 c        14265 g        13926 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc
    40141 aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc
    40201 tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg
    40261 ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc
    40321 ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca
    40381 tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc
    40441 tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc
    40501 attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc
    40561 cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa
    40621 tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc
    40681 tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc
    40741 agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta
    40801 tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc
    40861 gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc
    40921 acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg
    40981 aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc
    41041 gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca
    41101 ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg
    41161 tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct
    41221 tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata
    41281 tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata
    41341 gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg
    41401 agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca
    41461 tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg
    41521 gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa
    41581 gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga
    41641 aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa
    41701 gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat
    41761 ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg
    41821 aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat
    41881 tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga
    41941 aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc
    42001 accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca
    42061 aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt
    42121 tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa
    42181 gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga
    42241 aagctttgtg gcatggcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga
    42301 taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg
    42361 gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata
    42421 tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc
    42481 gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat
    42541 tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa
    42601 gtgtgccatc accacggacc actcgatgac agggtattac gatagccagc ttgttggcgg
    42661 cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct
    42721 gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa
    42781 aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg
    42841 cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg
    42901 gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc
    42961 aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt
    43021 aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt
    43081 catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg
    43141 tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc
    43201 aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca
    43261 tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac
    43321 acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac
    43381 gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat
    43441 tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca
    43501 cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt
    43561 gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt
    43621 agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg
    43681 acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg
    43741 atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt
    43801 accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg
    43861 gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt
    43921 ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg
    43981 atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc
    44041 cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga
    44101 tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca
    44161 ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc
    44221 gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt
    44281 tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc
    44341 ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg
    44401 acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag
    44461 cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg
    44521 gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca
    44581 cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg
    44641 gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg
    44701 cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca
    44761 aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat
    44821 atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg
    44881 ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg
    44941 gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg
    45001 tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc
    45061 agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct
    45121 ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca
    45181 gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg
    45241 ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt
    45301 ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg
    45361 ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct
    45421 tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca
    45481 aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca
    45541 gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaggaagt tacgtcgtaa
    45601 actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg
    45661 aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat
    45721 tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga
    45781 cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct
    45841 actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct
    45901 gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta
    45961 accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat
    46021 taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata
    46081 ctaaaacttt taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg
    46141 aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt
    46201 caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa
    46261 ccttcactcg caactagaat aactcccgct atcatcatta actttattta ttaccgtcat
    46321 tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta
    46381 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
    46441 actatttcac aaataattaa catcagcata atttccagca atctttgttt atttgcaatt
    46501 aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
    46561 ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
    46621 ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
    46681 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
    46741 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat
    46801 ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac
    46861 ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa
    46921 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
    46981 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
    47041 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
    47101 tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
    47161 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga
    47221 cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga
    47281 aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac
    47341 cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    47401 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    47461 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    47521 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    47581 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    47641 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    47701 tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    47761 tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    47821 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat
    47881 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    47941 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    48001 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    48061 aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc
    48121 acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    48181 tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga
    48241 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    48301 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    48361 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    48421 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    48481 tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat
    48541 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg
    48601 aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    48661 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    48721 cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    48781 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg
    48841 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    48901 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    48961 acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    49021 agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct
    49081 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    49141 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    49201 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    49261 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    49321 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    49381 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    49441 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    49501 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    49561 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    49621 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    49681 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    49741 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    49801 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    49861 tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    49921 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc
    49981 tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag
    50041 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    50101 aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga
    50161 taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    50221 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg
    50281 ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca
    50341 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    50401 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    50461 aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc
    50521 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg
    50581 atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga
    50641 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    50701 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    50761 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    50821 cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    50881 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    50941 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    51001 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    51061 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    51121 cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac
    51181 cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    51241 atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    51301 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    51361 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    51421 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    51481 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    51541 gttgttcatt accgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag
    51601 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    51661 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    51721 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    51781 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    51841 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    51901 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    51961 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    52021 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc
    52081 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    52141 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    52201 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    52261 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    52321 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    52381 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    52441 aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta
    52501 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    52561 cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    52621 gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc
    52681 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    52741 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    52801 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    52861 aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    52921 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    52981 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    53041 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag
    53101 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac
    53161 cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat
    53221 ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta
    53281 tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt
    53341 gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca
    53401 tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca
    53461 gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat
    53521 agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat
    53581 ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat
    53641 cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc
    53701 gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc
    53761 tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc
    53821 gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc
    53881 ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg
    53941 agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa
    54001 aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt
    54061 cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa
    54121 tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca
    54181 tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc
    54241 cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag
    54301 gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtctcg
    54361 ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt
    54421 aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca
    54481 aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag
    54541 gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg
    54601 agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca
    54661 cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc
    54721 tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc
    54781 gcctcgcgat ccagaactct ggtaacgtac tcttcacgat cgagatgcga ctgcaatttc
    54841 agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta
    54901 ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta
    54961 ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat
    55021 tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg
    55081 gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac
    55141 cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt
    55201 ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc
    55261 acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc
    55321 agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca
    55381 ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc
    55441 ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca
    55501 gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt
    55561 tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg
    55621 gtgagcgagt caggtaatgc tgagactttg gtcacgcctg atgacgacag ctgatacagt
    55681 tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc
    55741 agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc
    55801 ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa
    55861 cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg
    55921 accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc
    55981 gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc
    56041 catgttgtgc gctcaacgtc ggctcccggc gggagcggta cttccacctg accatagcgc
    56101 agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca
    56161 atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc
    56221 cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt
    56281 tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg
    56341 gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56401 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56461 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56521 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56581 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56641 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56701 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56761 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56821 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56881 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56941 tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc
    57001 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    57061 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    57121 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57181 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57241 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57301 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57361 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57421 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57481 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57541 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57601 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57661 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57721 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57781 cacctcttca cgccgtggac gttcaagcat ttttactcgc cgctcgctgc ggttagtcgc
    57841 gccaggcgca accggctcgc tagagagcgc ctggtcatag ctgataaacg acagactgga
    57901 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57961 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    58021 aggtttacct ttcagcgacg aggttaat
//
LOCUS       DAEEDS010000017        55430 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEEDS010000017 DAEEDS010000000
VERSION     DAEEDS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.017"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="KLH32_001098"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="KLH32_001098"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBE0138016.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KLH32_001099"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KLH32_001099"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBE0138017.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KLH32_001100"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KLH32_001100"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBE0138018.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="KLH32_001101"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="KLH32_001101"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBE0138019.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="KLH32_001102"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="KLH32_001102"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBE0138020.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="KLH32_001103"
     CDS             7826..9559
                     /locus_tag="KLH32_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBE0138021.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="KLH32_001104"
     CDS             9708..10559
                     /locus_tag="KLH32_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0138022.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="KLH32_001105"
     CDS             complement(10546..10887)
                     /locus_tag="KLH32_001105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBE0138023.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="KLH32_001106"
     CDS             complement(10889..11767)
                     /locus_tag="KLH32_001106"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBE0138024.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11769..14030)
                     /locus_tag="KLH32_001107"
     CDS             complement(11769..14030)
                     /locus_tag="KLH32_001107"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE0138025.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEI"
     gene            complement(14081..14401)
                     /locus_tag="KLH32_001108"
     CDS             complement(14081..14401)
                     /locus_tag="KLH32_001108"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBE0138026.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="KLH32_001109"
     CDS             complement(14416..15495)
                     /locus_tag="KLH32_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBE0138027.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="KLH32_001110"
     CDS             15804..16907
                     /locus_tag="KLH32_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBE0138028.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="KLH32_001111"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="KLH32_001111"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0138029.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="KLH32_001112"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="KLH32_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="KLH32_001113"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="KLH32_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="KLH32_001114"
     CDS             20366..20983
                     /locus_tag="KLH32_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBE0138030.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KLH32_001115"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KLH32_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBE0138031.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KLH32_001116"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KLH32_001116"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBE0138032.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KLH32_001117"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KLH32_001117"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBE0138033.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KLH32_001118"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KLH32_001118"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBE0138034.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KLH32_001119"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KLH32_001119"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBE0138035.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="KLH32_001120"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="KLH32_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBE0138036.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="KLH32_001121"
     CDS             30130..30738
                     /locus_tag="KLH32_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBE0138037.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KLH32_001122"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KLH32_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBE0138038.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="KLH32_001123"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="KLH32_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBE0138039.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="KLH32_001124"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="KLH32_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBE0138040.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQEHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="KLH32_001125"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="KLH32_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBE0138041.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="KLH32_001126"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="KLH32_001126"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBE0138042.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="KLH32_001127"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="KLH32_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBE0138043.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="KLH32_001128"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="KLH32_001128"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBE0138044.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="KLH32_001129"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="KLH32_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBE0138045.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KLH32_001130"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KLH32_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBE0138046.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="KLH32_001131"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="KLH32_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="KLH32_001132"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="KLH32_001132"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBE0138047.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="KLH32_001133"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="KLH32_001133"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBE0138048.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="KLH32_001134"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="KLH32_001134"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBE0138049.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KLH32_001135"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KLH32_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBE0138050.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="KLH32_001136"
     CDS             complement(44853..45152)
                     /locus_tag="KLH32_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBE0138051.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="KLH32_001137"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="KLH32_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="KLH32_001138"
     CDS             45905..46504
                     /locus_tag="KLH32_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBE0138052.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="KLH32_001139"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="KLH32_001139"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBE0138053.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KLH32_001140"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KLH32_001140"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBE0138054.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KLH32_001141"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KLH32_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBE0138055.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KLH32_001142"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KLH32_001142"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBE0138056.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KLH32_001143"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KLH32_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBE0138057.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KLH32_001144"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KLH32_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBE0138058.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="KLH32_001145"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="KLH32_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBE0138059.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="KLH32_001146"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="KLH32_001146"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBE0138060.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KLH32_001147"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KLH32_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBE0138061.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13506 a        15020 c        14159 g        12745 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct agatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggatgca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagagcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAEEDS010000018        53214 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEEDS010000018 DAEEDS010000000
VERSION     DAEEDS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53214
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.018"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(12..323)
                     /locus_tag="KLH32_001148"
     CDS             complement(12..323)
                     /locus_tag="KLH32_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBE0138062.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="KLH32_001149"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KLH32_001149"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="KLH32_001150"
     CDS             702..1055
                     /locus_tag="KLH32_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBE0138063.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="KLH32_001151"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="KLH32_001151"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBE0138064.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="KLH32_001152"
     CDS             complement(2811..3281)
                     /locus_tag="KLH32_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBE0138065.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="KLH32_001153"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="KLH32_001153"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBE0138066.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="KLH32_001154"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="KLH32_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE0138067.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="KLH32_001155"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="KLH32_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBE0138068.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="KLH32_001156"
     CDS             complement(5284..8433)
                     /locus_tag="KLH32_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBE0138069.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="KLH32_001157"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="KLH32_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBE0138070.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="KLH32_001158"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="KLH32_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBE0138071.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="KLH32_001159"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="KLH32_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBE0138072.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="KLH32_001160"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="KLH32_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBE0138073.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="KLH32_001161"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="KLH32_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBE0138074.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="KLH32_001162"
     CDS             14912..15289
                     /locus_tag="KLH32_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBE0138075.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="KLH32_001163"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="KLH32_001163"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBE0138076.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="KLH32_001164"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="KLH32_001164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBE0138077.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="KLH32_001164"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="KLH32_001165"
     CDS             18100..18429
                     /locus_tag="KLH32_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBE0138078.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="KLH32_001166"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="KLH32_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBE0138079.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="KLH32_001167"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="KLH32_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBE0138080.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="KLH32_001168"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="KLH32_001168"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBE0138081.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="KLH32_001169"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="KLH32_001169"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBE0138082.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23893)
                     /gene="aes"
                     /locus_tag="KLH32_001170"
     CDS             complement(22934..23893)
                     /gene="aes"
                     /locus_tag="KLH32_001170"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBE0138083.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="KLH32_001171"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="KLH32_001171"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBE0138084.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="KLH32_001172"
     CDS             complement(25482..27158)
                     /locus_tag="KLH32_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBE0138085.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="KLH32_001173"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="KLH32_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBE0138086.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="KLH32_001174"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="KLH32_001174"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBE0138087.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="KLH32_001175"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="KLH32_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBE0138088.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="KLH32_001176"
     CDS             complement(31206..32000)
                     /locus_tag="KLH32_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBE0138089.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="KLH32_001177"
     CDS             32138..32479
                     /locus_tag="KLH32_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE0138090.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="KLH32_001178"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="KLH32_001178"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBE0138091.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="KLH32_001179"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="KLH32_001179"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBE0138092.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="KLH32_001180"
     CDS             36395..37687
                     /locus_tag="KLH32_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0138093.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38246
                     /gene="cueR"
                     /locus_tag="KLH32_001181"
     CDS             37812..38246
                     /gene="cueR"
                     /locus_tag="KLH32_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBE0138094.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38220..38678)
                     /locus_tag="KLH32_001182"
     CDS             complement(38220..38678)
                     /locus_tag="KLH32_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBE0138095.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="KLH32_001183"
     CDS             complement(38675..39592)
                     /locus_tag="KLH32_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBE0138096.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="KLH32_001184"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="KLH32_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBE0138097.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="KLH32_001185"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="KLH32_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBE0138098.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="KLH32_001186"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="KLH32_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBE0138099.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="KLH32_001187"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="KLH32_001187"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE0138100.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="KLH32_001188"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="KLH32_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="KLH32_001189"
     CDS             43551..44237
                     /locus_tag="KLH32_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0138101.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46649
                     /locus_tag="KLH32_001190"
                     /pseudo
     CDS             44234..46649
                     /locus_tag="KLH32_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="KLH32_001191"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="KLH32_001191"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBE0138102.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="KLH32_001192"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="KLH32_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBE0138103.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVTPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="KLH32_001193"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="KLH32_001193"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBE0138104.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="KLH32_001194"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="KLH32_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBE0138105.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="KLH32_001195"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="KLH32_001195"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBE0138106.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53214
                     /locus_tag="KLH32_001196"
     CDS             52939..>53214
                     /locus_tag="KLH32_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBE0138107.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIR"
BASE COUNT        12828 a        13691 c        14023 g        12672 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgtgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttgctg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctggactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattattgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccaatt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccttggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctagaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaat
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga gctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgct
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacattggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgctg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggggat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtcttgcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggagttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaatgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgatgttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctataactat gattttcttc atgtgctaga tgaggactgt
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaaccaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg ggttacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattgatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaattaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct gggggtttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tcgt
//
LOCUS       DAEEDS010000019        53199 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEEDS010000019 DAEEDS010000000
VERSION     DAEEDS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53199
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.019"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            98..397
                     /locus_tag="KLH32_001197"
     CDS             98..397
                     /locus_tag="KLH32_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0138108.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            407..931
                     /gene="ygaP"
                     /locus_tag="KLH32_001198"
     CDS             407..931
                     /gene="ygaP"
                     /locus_tag="KLH32_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBE0138109.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(978..1382)
                     /gene="stpA"
                     /locus_tag="KLH32_001199"
     CDS             complement(978..1382)
                     /gene="stpA"
                     /locus_tag="KLH32_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBE0138110.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2050..2499
                     /gene="alaE"
                     /locus_tag="KLH32_001200"
     CDS             2050..2499
                     /gene="alaE"
                     /locus_tag="KLH32_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBE0138111.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2536..2880)
                     /locus_tag="KLH32_001201"
     CDS             complement(2536..2880)
                     /locus_tag="KLH32_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBE0138112.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3032..3262
                     /locus_tag="KLH32_001202"
     CDS             3032..3262
                     /locus_tag="KLH32_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138113.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3609..3854
                     /gene="nrdH"
                     /locus_tag="KLH32_001203"
     CDS             3609..3854
                     /gene="nrdH"
                     /locus_tag="KLH32_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBE0138114.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3851..4261
                     /gene="nrdI"
                     /locus_tag="KLH32_001204"
     CDS             3851..4261
                     /gene="nrdI"
                     /locus_tag="KLH32_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBE0138115.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4234..6378
                     /gene="nrdE"
                     /locus_tag="KLH32_001205"
     CDS             4234..6378
                     /gene="nrdE"
                     /locus_tag="KLH32_001205"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBE0138116.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6388..7347
                     /gene="nrdF"
                     /locus_tag="KLH32_001206"
     CDS             6388..7347
                     /gene="nrdF"
                     /locus_tag="KLH32_001206"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBE0138117.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7702..8904
                     /gene="proV"
                     /locus_tag="KLH32_001207"
     CDS             7702..8904
                     /gene="proV"
                     /locus_tag="KLH32_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBE0138118.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8897..9961
                     /gene="proW"
                     /locus_tag="KLH32_001208"
     CDS             8897..9961
                     /gene="proW"
                     /locus_tag="KLH32_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBE0138119.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10019..11011
                     /gene="proX"
                     /locus_tag="KLH32_001209"
     CDS             10019..11011
                     /gene="proX"
                     /locus_tag="KLH32_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBE0138120.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11203..12382
                     /locus_tag="KLH32_001210"
                     /pseudo
     CDS             11203..12382
                     /locus_tag="KLH32_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12506..13243
                     /gene="ygaZ"
                     /locus_tag="KLH32_001211"
     CDS             12506..13243
                     /gene="ygaZ"
                     /locus_tag="KLH32_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBE0138121.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13233..13568
                     /gene="ygaH"
                     /locus_tag="KLH32_001212"
     CDS             13233..13568
                     /gene="ygaH"
                     /locus_tag="KLH32_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBE0138122.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13659..14189
                     /gene="emrR"
                     /locus_tag="KLH32_001213"
     CDS             13659..14189
                     /gene="emrR"
                     /locus_tag="KLH32_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBE0138123.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14316..15488
                     /gene="emrA"
                     /locus_tag="KLH32_001214"
                     /pseudo
     CDS             14316..15488
                     /gene="emrA"
                     /locus_tag="KLH32_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15505..17043
                     /gene="emrB"
                     /locus_tag="KLH32_001215"
     CDS             15505..17043
                     /gene="emrB"
                     /locus_tag="KLH32_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBE0138124.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="KLH32_001216"
     CDS             complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="KLH32_001216"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBE0138125.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="KLH32_001217"
     CDS             complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="KLH32_001217"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBE0138126.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19401..19829)
                     /locus_tag="KLH32_001218"
     CDS             complement(19401..19829)
                     /locus_tag="KLH32_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0138127.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="KLH32_001219"
     CDS             complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="KLH32_001219"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBE0138128.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20540..20616)
                     /locus_tag="KLH32_001220"
     tRNA            complement(20540..20616)
                     /locus_tag="KLH32_001220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20580..20582),aa:Arg,seq:acg)
     gene            complement(20681..20757)
                     /locus_tag="KLH32_001221"
     tRNA            complement(20681..20757)
                     /locus_tag="KLH32_001221"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20721..20723),aa:Arg,seq:acg)
     gene            complement(20822..20898)
                     /locus_tag="KLH32_001222"
     tRNA            complement(20822..20898)
                     /locus_tag="KLH32_001222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20862..20864),aa:Arg,seq:acg)
     gene            complement(20963..21039)
                     /locus_tag="KLH32_001223"
     tRNA            complement(20963..21039)
                     /locus_tag="KLH32_001223"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21003..21005),aa:Arg,seq:acg)
     gene            complement(21043..21135)
                     /locus_tag="KLH32_001224"
     tRNA            complement(21043..21135)
                     /locus_tag="KLH32_001224"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21099..21101),aa:Ser,seq:gct)
     gene            complement(21451..21636)
                     /gene="csrA"
                     /locus_tag="KLH32_001225"
     CDS             complement(21451..21636)
                     /gene="csrA"
                     /locus_tag="KLH32_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBE0138129.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21871..24501)
                     /gene="alaS"
                     /locus_tag="KLH32_001226"
     CDS             complement(21871..24501)
                     /gene="alaS"
                     /locus_tag="KLH32_001226"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBE0138130.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24629..25129)
                     /gene="recX"
                     /locus_tag="KLH32_001227"
     CDS             complement(24629..25129)
                     /gene="recX"
                     /locus_tag="KLH32_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBE0138131.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25372..26433)
                     /gene="recA"
                     /locus_tag="KLH32_001228"
     CDS             complement(25372..26433)
                     /gene="recA"
                     /locus_tag="KLH32_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBE0138132.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26513..27010)
                     /gene="pncC"
                     /locus_tag="KLH32_001229"
     CDS             complement(26513..27010)
                     /gene="pncC"
                     /locus_tag="KLH32_001229"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBE0138133.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27155..28240)
                     /gene="mltB"
                     /locus_tag="KLH32_001230"
     CDS             complement(27155..28240)
                     /gene="mltB"
                     /locus_tag="KLH32_001230"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBE0138134.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28496..29058
                     /gene="srlA"
                     /locus_tag="KLH32_001231"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28496..29058
                     /gene="srlA"
                     /locus_tag="KLH32_001231"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29055..30014
                     /gene="srlE"
                     /locus_tag="KLH32_001232"
                     /gene_synonym="gutE"
     CDS             29055..30014
                     /gene="srlE"
                     /locus_tag="KLH32_001232"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBE0138135.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            30025..30396
                     /gene="srlB"
                     /locus_tag="KLH32_001233"
                     /gene_synonym="gutB"
     CDS             30025..30396
                     /gene="srlB"
                     /locus_tag="KLH32_001233"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBE0138136.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30400..31179
                     /gene="srlD"
                     /locus_tag="KLH32_001234"
     CDS             30400..31179
                     /gene="srlD"
                     /locus_tag="KLH32_001234"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBE0138137.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31284..31643
                     /gene="gutM"
                     /locus_tag="KLH32_001235"
                     /pseudo
     CDS             31284..31643
                     /gene="gutM"
                     /locus_tag="KLH32_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            31710..32483
                     /gene="srlR"
                     /locus_tag="KLH32_001236"
                     /gene_synonym="gutR"
     CDS             31710..32483
                     /gene="srlR"
                     /locus_tag="KLH32_001236"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBE0138138.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32476..33441
                     /gene="gutQ"
                     /locus_tag="KLH32_001237"
     CDS             32476..33441
                     /gene="gutQ"
                     /locus_tag="KLH32_001237"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBE0138139.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33438..34952)
                     /gene="norR"
                     /locus_tag="KLH32_001238"
     CDS             complement(33438..34952)
                     /gene="norR"
                     /locus_tag="KLH32_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBE0138140.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35139..36578
                     /gene="norV"
                     /locus_tag="KLH32_001239"
     CDS             35139..36578
                     /gene="norV"
                     /locus_tag="KLH32_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBE0138141.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36575..37708
                     /gene="norW"
                     /locus_tag="KLH32_001240"
     CDS             36575..37708
                     /gene="norW"
                     /locus_tag="KLH32_001240"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBE0138142.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37836..40088)
                     /gene="hypF"
                     /locus_tag="KLH32_001241"
     CDS             complement(37836..40088)
                     /gene="hypF"
                     /locus_tag="KLH32_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBE0138143.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40241..40768)
                     /gene="hydN"
                     /locus_tag="KLH32_001242"
     CDS             complement(40241..40768)
                     /gene="hydN"
                     /locus_tag="KLH32_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBE0138144.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40917..41927)
                     /gene="ascG"
                     /locus_tag="KLH32_001243"
     CDS             complement(40917..41927)
                     /gene="ascG"
                     /locus_tag="KLH32_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBE0138145.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(41941..42060)
                     /locus_tag="KLH32_001244"
     CDS             complement(41941..42060)
                     /locus_tag="KLH32_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBE0138146.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(42070..43779)
                     /gene="hycE"
                     /locus_tag="KLH32_001245"
     CDS             complement(42070..43779)
                     /gene="hycE"
                     /locus_tag="KLH32_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBE0138147.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43797..44720)
                     /gene="hycD"
                     /locus_tag="KLH32_001246"
     CDS             complement(43797..44720)
                     /gene="hycD"
                     /locus_tag="KLH32_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBE0138148.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44723..45418)
                     /locus_tag="KLH32_001247"
                     /pseudo
     CDS             complement(44723..45418)
                     /locus_tag="KLH32_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3);
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            complement(45587..46198)
                     /gene="hycB"
                     /locus_tag="KLH32_001248"
     CDS             complement(45587..46198)
                     /gene="hycB"
                     /locus_tag="KLH32_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBE0138149.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46323..46784)
                     /gene="hycA"
                     /locus_tag="KLH32_001249"
     CDS             complement(46323..46784)
                     /gene="hycA"
                     /locus_tag="KLH32_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBE0138150.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            46996..47346
                     /gene="hypA"
                     /locus_tag="KLH32_001250"
     CDS             46996..47346
                     /gene="hypA"
                     /locus_tag="KLH32_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE0138151.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            47350..48222
                     /gene="hypB"
                     /locus_tag="KLH32_001251"
     CDS             47350..48222
                     /gene="hypB"
                     /locus_tag="KLH32_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBE0138152.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48213..48485
                     /gene="hypC"
                     /locus_tag="KLH32_001252"
     CDS             48213..48485
                     /gene="hypC"
                     /locus_tag="KLH32_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBE0138153.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            48485..49606
                     /gene="hypD"
                     /locus_tag="KLH32_001253"
     CDS             48485..49606
                     /gene="hypD"
                     /locus_tag="KLH32_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBE0138154.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            49603..50613
                     /gene="hypE"
                     /locus_tag="KLH32_001254"
     CDS             49603..50613
                     /gene="hypE"
                     /locus_tag="KLH32_001254"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBE0138155.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            50687..52765
                     /gene="flhA"
                     /locus_tag="KLH32_001255"
     CDS             50687..52765
                     /gene="flhA"
                     /locus_tag="KLH32_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBE0138156.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(52802..53155)
                     /locus_tag="KLH32_001256"
     CDS             complement(52802..53155)
                     /locus_tag="KLH32_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBE0138157.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT        12633 a        14309 c        13619 g        12638 t
ORIGIN      
        1 tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg catttttagt
       61 ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca
      121 ggccagtacc gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct
      181 gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac
      241 cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat
      301 caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc
      361 cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac
      421 catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg
      481 tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt
      541 gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca
      601 ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga
      661 aatattttta ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt
      721 aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat
      781 attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt
      841 tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt
      901 agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg
      961 acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc
     1021 tgagcaattg gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca
     1081 tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca
     1141 gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc
     1201 caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca
     1261 cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag
     1321 aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac
     1381 ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg
     1441 atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca
     1501 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
     1561 gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga
     1621 caaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt
     1681 gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg
     1741 acaaacaccg aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa
     1801 attaaaggca agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa
     1861 aatccacaat gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa
     1921 ataaaaattt ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca
     1981 gatggcaatg agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg
     2041 agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg
     2101 atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc
     2161 ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg
     2221 ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc
     2281 tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg
     2341 gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac
     2401 atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga
     2461 ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca
     2521 tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc
     2581 aacacgcgaa ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata
     2641 gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt
     2701 tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc
     2761 gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac
     2821 atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat
     2881 atttgatcct cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc
     2941 tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg
     3001 aaaattacat cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt
     3061 tgatgatggc gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc
     3121 tgtattgaag tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc
     3181 tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc
     3241 cgcgcgcgat gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt
     3301 gggtacagca gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta
     3361 gtgcaaaaat gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt
     3421 ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg
     3481 atcactattt gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg
     3541 tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa
     3601 tacgaatcat gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca
     3661 aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg
     3721 aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg
     3781 atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg
     3841 cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt
     3901 tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca
     3961 ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc
     4021 ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg
     4081 cgttattgct tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat
     4141 tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga
     4201 tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg
     4261 acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca
     4321 ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg
     4381 cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa
     4441 ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt
     4501 gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac
     4561 accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga
     4621 gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc
     4681 gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag
     4741 cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg
     4801 attggtcggg cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt
     4861 ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct
     4921 ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc
     4981 gcgcgtcagg gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt
     5041 ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc
     5101 gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt
     5161 tcgccctatg acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa
     5221 cactatgacg aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat
     5281 ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa
     5341 gacacagtaa accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca
     5401 gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca
     5461 ggccatgata tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc
     5521 gatttttccc gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt
     5581 catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg
     5641 gggcagatga atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa
     5701 gccctggatt tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg
     5761 atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc
     5821 agtggtgaat atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt
     5881 ggcgaactgt ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg
     5941 cgcgacgacg tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc
     6001 gggtctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta
     6061 gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac
     6121 gaaaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc
     6181 tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat
     6241 accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa
     6301 acgctctatt acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc
     6361 gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg
     6421 aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg
     6481 ctaccagaaa aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta
     6541 gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat
     6601 gttatcggcg cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta
     6661 tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg
     6721 ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg
     6781 cagcgaaaag ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa
     6841 atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat
     6901 ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat
     6961 gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct
     7021 ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac
     7081 aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa
     7141 gcgtttctct gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt
     7201 cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat
     7261 gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa
     7321 acagaagatg aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag
     7381 cagggaaata attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca
     7441 ctgatactct gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct
     7501 atgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc
     7561 ggcgatttgc tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct
     7621 cagtatgtta gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa
     7681 ataaaggaat cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata
     7741 tttggtgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa
     7801 attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa
     7861 ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt
     7921 ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc
     7981 aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag
     8041 tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg
     8101 gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg
     8161 ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg
     8221 ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg
     8281 gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa
     8341 catcagcgca ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac
     8401 cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc
     8461 aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg
     8521 ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc
     8581 ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt
     8641 atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta
     8701 acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa
     8761 acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc
     8821 gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat
     8881 cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga
     8941 cagtgccgcg caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg
     9001 tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga
     9061 tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt
     9121 tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa
     9181 cggtttccag caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct
     9241 catcacctgg cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc
     9301 catcggcgca atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc
     9361 cctgctgttc tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg
     9421 agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta
     9481 tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat
     9541 catctttgcg ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc
     9601 ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt
     9661 tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc
     9721 cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact
     9781 tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat
     9841 cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg
     9901 caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta
     9961 actctgcact tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat
    10021 gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc
    10081 tgccgatctg ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga
    10141 aaccttccag acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa
    10201 acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt
    10261 caccgccgtg aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa
    10321 gaaattttat cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa
    10381 gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc
    10441 caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta accctggctg
    10501 gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac
    10561 tcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg
    10621 caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg
    10681 gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga
    10741 taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc
    10801 caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt
    10861 gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg
    10921 cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg
    10981 ggtgaatgag gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg
    11041 cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta
    11101 caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta
    11161 ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat
    11221 gagcttagcc cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt
    11281 aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt
    11341 tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt
    11401 gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca
    11461 gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg
    11521 gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg
    11581 gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg
    11641 atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc
    11701 ttttgggttg cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca
    11761 caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg
    11821 ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac
    11881 ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc
    11941 gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg
    12001 gcgggcggtt ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt
    12061 actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat
    12121 tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg
    12181 aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg
    12241 cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt
    12301 ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca
    12361 tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag
    12421 ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct atttataata
    12481 ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc
    12541 ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt
    12601 ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt
    12661 tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc
    12721 cgggagtagt ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt
    12781 gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct
    12841 atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa
    12901 taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg
    12961 ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc
    13021 cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc
    13081 tttccagcgc aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt
    13141 aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc
    13201 gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt
    13261 tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta
    13321 atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct
    13381 cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt
    13441 tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca
    13501 ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga
    13561 ttatataacc cacaagaatc atttttctaa aacaatacat ttacttcatt tgtcactgtc
    13621 gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca
    13681 ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc
    13741 ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc
    13801 tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg
    13861 aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg
    13921 cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata
    13981 acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg
    14041 ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa
    14101 aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag
    14161 acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat
    14221 aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc
    14281 tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa
    14341 ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct
    14401 tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt
    14461 tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt
    14521 ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc
    14581 tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg
    14641 cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata
    14701 ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc
    14761 tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca
    14821 gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc
    14881 tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg
    14941 cctagctggc gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc
    15001 gtgccgtgca accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag
    15061 ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg
    15121 gtcagccggt caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag
    15181 tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga
    15241 ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac
    15301 agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta
    15361 accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg
    15421 cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg
    15481 ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg
    15541 gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt
    15601 gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg
    15661 tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg
    15721 gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg
    15781 tcttgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag
    15841 gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg
    15901 ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt
    15961 tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc
    16021 atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt
    16081 gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt
    16141 atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag
    16201 gaaattatca ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag
    16261 ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc
    16321 ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg
    16381 cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg
    16441 gtagggatta ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat
    16501 atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc
    16561 tatacctttg aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg
    16621 tttgcggtgg tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg
    16681 gaacgactgg cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc
    16741 ggcacgtcga taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg
    16801 actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg
    16861 cttgggatga cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg
    16921 ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg
    16981 gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac
    17041 taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt
    17101 tactgactag atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct
    17161 gttgatgcgt acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga
    17221 gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg
    17281 cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg
    17341 cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat
    17401 ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt
    17461 atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac
    17521 ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc
    17581 catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat
    17641 ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg
    17701 catttgttat catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt
    17761 tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt
    17821 tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc
    17881 agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct
    17941 gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg
    18001 ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta
    18061 atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc
    18121 agtaacggga actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct
    18181 tcaaggatca cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc
    18241 ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca
    18301 tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg
    18361 ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga
    18421 atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta
    18481 ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta
    18541 aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta
    18601 ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg
    18661 cactcggttt tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc
    18721 gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata
    18781 acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac
    18841 ttcgcttgcc agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc
    18901 atcagcgcgc cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg
    18961 ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata
    19021 cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc
    19081 ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct
    19141 tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga
    19201 cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc
    19261 tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc
    19321 gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga
    19381 ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca
    19441 actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag
    19501 atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat
    19561 aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat
    19621 gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta
    19681 cccattgttg ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc
    19741 accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac
    19801 aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg
    19861 ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt
    19921 gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga
    19981 cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg
    20041 cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac
    20101 ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg
    20161 cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa
    20221 ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt
    20281 actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat
    20341 ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct
    20401 cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg
    20461 aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa
    20521 ggaaattttt tggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    20581 gtagccgagt actctatcca gctgagctac ggatacatcg ggaaacttac tttactgcag
    20641 attttttgat accgctacta aagccgtatc aagtaagaga tggtgcatcc gggaggattc
    20701 gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc
    20761 gggaaactta ctttactgca gattttttga taccgctact aaagccatat caagtaagag
    20821 atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga gtactctatc
    20881 cagctgagct acggatgcat cgggaaactt actttactgc agattttttg ataccgctac
    20941 taaagccgta tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc gaccgctcgg
    21001 ttcgtagccg agtactctat ccagctgagc tacggatgca aatggcggtg aggcggggat
    21061 tcgaaccccg gatgcagctt ttgaccgcat actcccttag caggggagcg ccttcagcct
    21121 ctcggccacc tcaccacacg cctcttacga gtgcttcgaa gaacttgttt atcgctcatc
    21181 atcgctgcgt ggcgcacata ttactttctg agacttataa gtcaaacaat ttttcccaca
    21241 cttttatcgt ttgcacactt cacgctcaat tagtctgtaa aagcgtcaaa aagagtgttt
    21301 tatctacaga aaaacggagg tctggcagat agcagtaatg caaaaaaatg gagacttaag
    21361 ttgaatgaac gggagtaaag cgaaaagact atagagtgaa ggagaaattt tgagggtgcg
    21421 tctcaccgat aaagatgaga cgcggaaaga ttagtaactg gactgctggg atttttcagc
    21481 ctggatacgc tggtagatct cttcacggtg aacagaaact tccttcgggg catttacgcc
    21541 aatacgtacc tggttgccct ttacccctaa aactgtcacg gtgacctcat ccccaatcat
    21601 gagggtctca ccaactcgac gagtcagaat cagcattctt tgctccttga aagattaaaa
    21661 gagtcgggtc tctctgtatc ccggcattat ccatcatata acgccaaaaa gtaagcaatg
    21721 acaaacacat tacatgtaag cagtcatggc attacattct gttaaaccta agtttagccg
    21781 atatacacaa cttcaacctg actttatcgt tgtcgatagc gttaatgcga atgccgtgaa
    21841 gcgagtccac ggcattgcct gacgcttata ttattgcaat ttcgcgctga cccagccttt
    21901 cacactggct aacgctgcag gtaaggccgc agcatccgta ccaccggctt gcgccatgtc
    21961 aggacgtcca ccacccttgc cgcccacctg ctgagcgacc ataccaatca gttcccctgc
    22021 tttcacacga tctgtgacgt ccttagatac gcctgcaatc agagaaacct taccttcggc
    22081 taccgttgcc agcacgataa ttgtcgaccc cagctgattt tttaaatcgt caaccatggt
    22141 acgcaacatt ttcggctcaa caccgctaag ctcgctaacc aacagcttaa caccattaac
    22201 atcaattgcc ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg
    22261 ttgtagttct ttttctaact gacgcgtacg ttccagtact gagcgcactt tatccgccag
    22321 attattgcta tcgcctttca gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg
    22381 aacggtggtg atagcgcctt ctccggttac cgcttcgata cgacgaacgc ctgcagcagt
    22441 acccgattca gagatgatgc ggaacagacc aatatcacca gtgcggctgg cgtgagtacc
    22501 gccacacagc tcggtggaga aatcgcccat gctcagcacg cgtacacgct catcatactt
    22561 ctcgccgaac agcgccatcg cacctttcgc tttcgccgct tcgagattca tgatgttggt
    22621 ttcgatcggc aaattgcgac gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc
    22681 ttccggtttc atcgcttcgt tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag
    22741 tgaacctttc tgcgatacat gagtccccag aacctggcgc agcgcagcgt gcatcaggtg
    22801 cgttgcggag tgattcagac gaatacgggc gcgacgagcc tcatcaatat cagcctgcac
    22861 agcgtcgccc actttcagag aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc
    22921 gtatttctgc gtatcttcca ccgcgaagga gaagttagcg cctttcagtt cgcctttatc
    22981 accaacctga ccgccggatt ccgcatagaa tggcgtttga tccagcacga ccacagcttc
    23041 ctggcctgca ttgatggcat caaccgcttt accatcaaca aacagcgcgg tcactttgcc
    23101 gttcagttcc agatggtcat agcctttaaa ttcagatgca ctatcaacac ggatcattgc
    23161 gttgtaatcg gcaccaaagc cgctggcttc gcgcgcgcga cgacgctgct cttccattgc
    23221 tgcttcaaaa ccagcttcgt caactttgat gttgcgctca cgacaaacat cagccgtcag
    23281 gtcaaccggg aagccatagg tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc
    23341 accagaaagt tttgccagct cttcatccag caacgccaga ccgcgctcca gagtacgggc
    23401 aaactgctct tcttcagtct tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc
    23461 ttcacccgca gagcccataa cgtcgatcag cggaccaacc agtttgtaga agaaggtttc
    23521 tttcgcgccg agcatattgc cgtgacgcac tgcgcgacga atgatacgac gcagtacata
    23581 accacggttt tcattggacg gcattacgcc atccgcgatc aggaacgcac aagaacgaat
    23641 gtggtcagcg attacgcgca gcgatttatt gctcagatcg gtcgcgccag tgactttcgc
    23701 taccgcctgg atcaacgtgc ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag
    23761 caccgcagca atacgctcca gacccatacc ggtatctaca gacggcttcg gcagcggttc
    23821 catcgtgcca tctgcctggc ggttgaactg catgaagacg atgttccaga tctcaatgta
    23881 gcggtcgccg tcttcttccg gacttcccgg agggccaccc caaatgtggt cgccgtgatc
    23941 gtagaagatt tcggtgcacg ggccgcacgg accagtgtca cccatctgcc agaagttgtc
    24001 agatgcgtat ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc gcgggatccc
    24061 gacttctttt tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg taacccacag
    24121 acgctctttc ggcagggcaa accatttttc gctggtcagc agttcccatg caaactgaat
    24181 ggcatcgtgt ttgaaatagt cgccgaagct gaagttgccc agcatttcga agaaggtatg
    24241 gtgacgcgcg gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg cacgcacgca
    24301 gcgttgggaa gtggtagcgc gggaataatt acgcttgtcg agcccaagga acacatcctt
    24361 gaactggttc atcccggcgt tggtaaacaa caaagttggg tcgttatggg gtaccaggga
    24421 gctgctggca actacctgat gtcccttact atggaaaaag tcgagaaacg cctgacggat
    24481 ctcagcggtg ctcttgctca taattatcct gaaatcaagc taacgaaata tcgccaccag
    24541 ctccagcgtg tcttaaccgc cgggctggta actgaaaagt gggaataaga taagttttct
    24601 tgactgggaa gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa atatcctgga
    24661 tatcttccat cagatagcca cgatagagca gaaaacgctg gatcttaact ttttctgaaa
    24721 agacagttgg caaaggttca ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc
    24781 agtcgatgtc acattcacgc atcgcttttt ctgtcgcttc gcgggaaata cctttctgat
    24841 tcagttcctg acgaatacgt gcaggtccat accctttgcg gctacggctg gcgataaagc
    24901 gcgcaacaaa tcggctgtca tcgagatagc catgttcatg gcaccaggca ataacgcgct
    24961 cgtaatcttc tgccgtagca tcaatctctt ctgggccatt tttgcccata atcggtgccg
    25021 cgagtttacg tcgcagttct tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac
    25081 gatccaacag gcgagcatat gccgggcgac gggatgttga ttctgtcatg gcatatcctt
    25141 acaacttaaa aaagcaaaag ggccgcagat gcgccccagg ttttgctgat aaacagattt
    25201 aatagtcaat gagtatgcag aatgttcccg caggcccaat tcgttagagg atcgaggtga
    25261 gagtaatttt tcacgccagt gctggcctgt tcccctcacc ctaaccctct ccccaaaggg
    25321 gcgaggggac cgtccgtgca cacattatct attatcatca ataacgaacg attaaaaatc
    25381 ttcgttagtt tctgctactc cttcgctgtc atctacagag aaatccggcg ttgagttcgg
    25441 gttgctcagc agcaactcac gtactttctt ctcgatctct ttcgcggttt ccgggttatc
    25501 tttcagccag gcagttgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta
    25561 ccacgcgcct gctttctcga tcagcttctc ttttacgccc aggtcaacca gttcgccgta
    25621 gaagttgata ccttcgccgt agaggatctg aaattcagcc tgtttaaacg gtgcagcgat
    25681 tttgttcttc accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac
    25741 cgcgccgata cgacggatgt cgagacgaac agaggcgtag aatttcagcg cgttaccacc
    25801 ggtagtggtt tccgggttac cgaacatcac accaattttc atacggatct ggttgatgaa
    25861 gatcagcagc gtgttggact gcttcaggtt acccgccagc ttacgcatcg cctggctcat
    25921 catacgtgcc gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg
    25981 cgtcagtgcc gccacggagt caacgacgat aacgtctact gcgccagaac gcgccagggc
    26041 gtcacagatt tccagtgcct gctcgccggt atccggctgg gagcacagca ggttatcgat
    26101 atcgacgccc agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc
    26161 acaggtttta ccttcacgct gcgctgcggc gatcacctgc agcgtcagcg tggttttacc
    26221 ggaagattcc ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag
    26281 cgcgatatcc agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc
    26341 acccaggcgc atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc
    26401 caacgctttc tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac
    26461 cggatagtca atatgttctg ttgaaacaat tatactgtat gctcatacag tatcaagtgt
    26521 tttgtagaaa ttgttgccac aaggtctgca atgcatacgc agtagcctga cgacgcaccg
    26581 catcacggtc gccgctgaag cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa
    26641 aagcaaacca gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac
    26701 cactaataga cacggcataa tcagcacgag ccgctttcag tgcacctatc gccatttcca
    26761 ccacgacggg ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca
    26821 tctgcgcttt ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac
    26881 cggcaatatc ggtaatcact ttcgctaccc aaccaccggt acaagactcg gcagttgtta
    26941 cggttgcgcc acgggctttc agcgcctgcc caacctgttc gcttaactgc atcagttcac
    27001 tgtcagtcat cacaacctct gtcagtcaaa aatttatgcc ggacaagata gcactttcgt
    27061 caggaagatg gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc
    27121 cagagggaag ctcacgcccc cctcttgtaa atagctactg tactcgcgcc agcgccacgg
    27181 cttgtcctaa ctgccagacc gccattgcgt aatgggtgct gtggttgtaa cgggtgatgg
    27241 tgtagaagtt cggcagaccg taccagtact ggtagccggt gccaacatcc agacgcagca
    27301 ggctggcttg ttgatggttg cccagcggct gctgtggcgt taaacctgcg gcggcaagct
    27361 gcgaaatgct gtacttagtt ttgaaaccat ttggcaagcc tggagcctga ccgtttgcca
    27421 ttatcgcgac ctgatcgcct ttcacccagc cgtgcgcttt gaaatagttc gccacgctac
    27481 cgatcgcatc aaccggatcc cacaggttga tatgcccgtc gccgctgaaa tctaccgcat
    27541 attgtttgta agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt
    27601 tcagattgag cggatcgtcc tgctcgtcgc gcgccatcag caggaaggtt tccagctcgc
    27661 cagagaaata ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga
    27721 tgcgagtttt ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa
    27781 tttccggcgg tacgccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc
    27841 agaaaaccac accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacgcagcc
    27901 atgcgccgtt cggacctgat ggcggtttca ccgatgtggt tggtgcctgg ttatccatca
    27961 gccgcagtac cgaatccaga cgcttcgcct gggagagaat ttcctgcaac tgctgacgat
    28021 cgaaaccgtg tttgttcacc attttgtcga tgaactgctg ggcattcggg ttattagcga
    28081 aatcgccgcc catctgcatc acattgtgct gcggctcaag caggaagccg ccagacggcg
    28141 ttccggtggt cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca
    28201 caaaaagggg aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat
    28261 tcaaccaggg gcaagtatgg taaagcatca caccccgcac aaggaagcgg tagtcactgc
    28321 ccgatacgga ctttacataa ctcaactcat tcccctcgct atacttttat tcaaactttc
    28381 aaattaaaat atttatcttt cattttacga tcaaaataac acttttaaac ctttcaacct
    28441 gattagatta ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat
    28501 agaaaccatt actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt
    28561 gtttaccggg atggtgaccg gcattcttcc gctgttgatt agcctgcagg ttatcatgaa
    28621 cgcactgatt aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg
    28681 taaccctgtt tcccgttacc tactgttacc gtgcattggc acgtttgtct tttgcaatcc
    28741 gatgacctta agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc
    28801 ggcctcttat agctgccact caatgaatgg cctcttcccc catatcaacc ctgcgaactg
    28861 tttgtttatc ttggcattgc cagcggtctg acaacgctga acctgccact tggcccactg
    28921 gcggtgagtt atctgctggt tggtctggtc accaatttct tccgcggctg ggtgaccgat
    28981 ctgaccaccg ccatttttga gaaaaagatg ggcattcaac ttgaacaaaa agttcacctg
    29041 gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg aacgggtggc tggggcggcc
    29101 cgcttgagct ggaagccacg ccgggcaaaa aaatcgtcta tatcaccgcc ggtacccggc
    29161 ctgcgattgt tgacaaactg gcacaactta ctggctggca ggctattgac ggatttaaag
    29221 aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc ggcacattac
    29281 gctgcggcat ctatccgaaa cgacgtattc ccaccattaa tatccactcg acgggcaagt
    29341 ccggtccgct ggcgcagtac attgtggaag atatttatgt ctctggcgta aaagaagaaa
    29401 acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt gactatgaca
    29461 ccagtaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga atgggcatgg
    29521 ggtccgccgt tgccgtgttg tttcagtctg gtcgtgacac catcgacact gtattaaaaa
    29581 ccattctgcc gtttatggca ttcgtctcgg cgctcattgg catcattatg gcttctggcc
    29641 ttggtgactg gattgcccac ggtcttgctc cgctagcgag ccatccactg ggtctggtca
    29701 tgctagcgct catctgctcc ttcccactgc tttcaccttt cctcggccca ggcgcagtta
    29761 tcgcacaggt tatcggcgta ttgattggcg tgcagattgg tctcggcaat attccgccgc
    29821 atctggcttt accggcactg tttgccatca acgcgcaggc ggcttgcgac ttcatcccgg
    29881 tcggtttgtc gctggcggaa gcccgtcagg acacggttcg cgtcggtgtc ccttctgtac
    29941 tggtgagccg ctttttaacc ggcgcgccga ctgtactgat cgcctggttt gtctccagtt
    30001 ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat cacccgtatc
    30061 ggcgcgagtg ccactgacgc cctcagcgac cagatgctca tcacctttcg tgaaggcgcg
    30121 ccagcggacc tcgaagagta ttgcttcatt cattgccacg gcgagttgaa aggtgcactc
    30181 catcccggtt tgcaattttc actcgggcag catcgctatc cggtgaccgc tgttggcagc
    30241 gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga tggtttaaac
    30301 gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tacccgacga tatcgcgccg
    30361 ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt tgccgttgtc
    30421 atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg gtctggctgc cgaggggtat
    30481 cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca agaaattaac
    30541 gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg acgccactag cgagcaaagc
    30601 gttctggcgc tctctcgtgg ggtagatgaa atctttggtc gcgtggattt gctggtctat
    30661 agcgccggaa tagcaaaagc agcctttatc agcgacttcc agctcggcga ttttgaccgt
    30721 tcgctacaag tgaatctggt gggttatttc ctgtgtgccc gtgaattttc gcgtttgatg
    30781 atccgcgacg ggattcaggg tcgcattatt cagatcaact cgaaatccgg caaagtgggc
    30841 agcaaacaca actctggcta cagcgcagcg aaatttggtg gcgtcgggct gactcaatca
    30901 ctggcgctgg atctggcgga gtacggcatt acggtacatt cactgatgct cggtaacctg
    30961 ctgaaatcgc ggatgttcca gtcactgttg ccacaatacg cgaccaagct gggtatcaaa
    31021 ccggatcaag tcgagcagta ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat
    31081 caagatgtgc tgaatatgct gctgttctac gccagtccta aggcgtcgta ctgcaccgga
    31141 cagtcgatca atgtcaccgg cggtcaggtg atgttctgat caacagcgga gatccgttaa
    31201 ggatctccgt gagactatag aatgcctgat gcgctacgct catcaggcat acaggacttc
    31261 cgccactaca ttaaggaaaa gttatggtat ccgcactcat caccgtcgcc gttatcgcct
    31321 agtgtgcgca actggcctta ggcggctggc aaatttctcg ttttaaccgt gccttcgaca
    31381 cactatgcca gcaagggcgg gttggcgtgg gccgttccag cgggcgcttt aaaccgcggg
    31441 tcgtggtcgc catcgcgctg gacgatcagc agtgcatcgt cgacaccttg tttatgaaag
    31501 gactgactgt cttcgcccga ccgcaaaaaa ttcccgcaat taccggcatg catgcgggtg
    31561 atttacagcc cgatgtgatc tttccccatg atccactatc acagaatgct ctatcattgg
    31621 cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta cttgcttgcg aagttatcat
    31681 tttgaaacct aaatcaggta atcacgccca tgaaacctcg tcagcgtcag gccgccattc
    31741 tggagtatct gcaaaagcag ggtaaatgct cggttgaaga attggcgcaa tactttgaca
    31801 ccacaggcac aaccattcgc aaagatctgg tcattctgga acatgccgga accgtcattc
    31861 gtacttatgg cggagtggtg ttgaataaag aggaatccga tccgcctatc gatcataaaa
    31921 cgctcatcaa cacccacaag aaagagctga ttgcagaagc tgccgttagt tttatccatg
    31981 atggcgattc gatcattctt gatgctggca gtaccgtttt gcagatggtt cccctgctct
    32041 cgcgctttaa taacatcacg gtgatgacca acagcctgca tatcgtcaat gcgctatccg
    32101 aactggataa cgaacaaact atcctgatgc caggcggaac atttcgcaaa aaatcggcct
    32161 catttcacgg gcaactggca gagaatgcct tcgagcattt caccttcgat aaattgttta
    32221 tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac gttcaacgag gtttataccg
    32281 tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt gatggcggac tcatcaaagt
    32341 ttggccgtaa aagccccaac gtagtttgca gtcttgaaag cgtcgataag ctaattaccg
    32401 acgcaggtat cgatccggcg tttcgtcagg cgctggaaga gaaagggatc gatgtgatca
    32461 taaccggaga gagcaatgag tgaagcacta ctgaacacgg gacgtcagac gttaatgctg
    32521 gagttgcagg aagcaagccg tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc
    32581 aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg gaattggcaa atcgggccac
    32641 attggtaaga aaatcgccgc aacacttgcc agtaccggca caccggcttt ttttgtccat
    32701 ccggcagaag cactgcacgg cgatctgggg atgattgaaa gccgcgatgt gatgctgttt
    32761 atctcttact ccggtggcgc gaaggaactg gatctgatta ttccgcgtct ggaagataaa
    32821 tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac cgctgggcct ggcggcgaaa
    32881 gcggtgctgg atatctccgt agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc
    32941 agcaccgtca ataccctgat gatgggtgac gcgctggcga tggcggtcat gcaggcgcgc
    33001 ggttttaatg aagaagattt tgcccgctcc cacccagctg gggcactggg cgctcgcttg
    33061 ctgaataaag tgcatcatct gatgcgtcgt gatgacgcta tcccacaggt agcgttaacc
    33121 gccagcgtga tggatgcgat gctggaactc agccgcaccg gtctggggct ggtggcggta
    33181 tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg gcgatttacg tcgctggctg
    33241 gtaggcggcg gcgcactcac cacgccagtt aatgaagcga tgacgacagg cggcaccaca
    33301 ttacaggcac aaagtcgcgc catcgacgcc aaagaagttc tgatgaaacg caaaatcact
    33361 gccgcaccag tggtagatga aaacggcaaa ctcaccggcg caattaacct gcaggatttc
    33421 tatcaggccg ggattattta atccttcaat cccagacgtt tcgccagccg atgcaggttg
    33481 gcgacgtcag tttccagcat tcgcgcacag gctgcccagt tgtgatgatt ttgtgccagt
    33541 gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca
    33601 acgagcaccg ccgccgcttc tggcggcggc aacgtcacct caggaaaagc aaaatgttgc
    33661 gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg ccagcactac cgcccgatga
    33721 atagcatgtt ccagttcgcg cacgtttccc ggaaaactgt agtgttgcag taaatttcgc
    33781 gccccggcac ttaataccac gcgggagagc ccctgccgca aacgacactg ctcgcagaaa
    33841 taccccgcca gcagaatgac atcatcgccc cgctcacgca gcggcggcac cgaaagtgga
    33901 aacacgctca ggcgatgaaa caaatcggcg cggaatcgcc ctgccagcac ctcttcgcgt
    33961 aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca
    34021 acgcgctgaa tatcgccata ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac
    34081 aactcgccga tctcatcgag aaacagagta ccgttatccg ccatttcaaa cttcccgctg
    34141 cggttactga tagcgccagt aaacgctcct ttcacatgcc cgaacaactc actttccgcc
    34201 acactttccg gcagtgcggc acagttgaga tagaccagcg gattcaccgc ccgtggcgag
    34261 gcttcatgaa tcgctttcgc caccagctcc ttaccggttc ccgtctcacc gctgatcaga
    34321 acgttgagat cggacgccgc cacaatctca atctcttttt tcagttgcgt catgccaggg
    34381 gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg gcgtggcatc gcctggcagc
    34441 atattctggc tttccagttg ttcaatcagc aacgcattgc ttaacgctcc cgccgccagc
    34501 gcggcaatca gccgtaactc ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg
    34561 tcgagcgtca gtgcgccgat caggttttgt ccgacaaaca atggcagacc aacgcaggcg
    34621 tgaaccttca gactctcctg cccaggaatc aaaccgtcat agggatcggg caattcgctg
    34681 tctgcgggaa agcgcaccac atccccggcg cgggcaatcg cttccagccg tggatgccct
    34741 tccagcgcaa agcgtctacc gagtacatcc tttgccagac cgtcgatggc aagcggaata
    34801 aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc actccagcac ctgacgtagc
    34861 gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg
    34921 atattcgcca gcacatcaac ggaaaaactc atctttgcct cactgtcaat ttgactatag
    34981 atattgtcat atcgaccatt tgattgatag tcattttgac tactcattaa tgagcataat
    35041 tttatttata gagtaaaaac aatcagataa gaaactggca cgcaatctgc aattagcaag
    35101 acatcttttt agaacacgct gaataaattg aggttgctat gtctattgtg gtgaaaaata
    35161 acattcattg ggtgggtcaa cgtgactggg aagtgcgtga ttttcacggc acggaatata
    35221 aaacgctgcg cggcagcagc tacaacagct acctcatccg cgaagaaaaa aacgtgctga
    35281 tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg
    35341 atctggcgga tatcgattac atcgtgatta accatgcaga agaggaccac gccggggcgc
    35401 tgaccgaact gatggcgcaa attcccgata cgccgatcta ctgtactgcc aacgctatcg
    35461 actcgataaa tggtcatcac catcatccgg agtggaattt taatgtggtg aaaactggcg
    35521 acacgctgga tatcggcaac ggcaaacagc tcatttttgt cgaaacacca atgctgcact
    35581 ggccggacag catgatgact tacctgacag gcgacgcggt gctgttcagt aacgatgctt
    35641 tcggtcagca ctactgcgac gagcatctgt tcaacgatga agtggatcag acggagcttt
    35701 tcgagcagtg ccagcgttac tacgccaata tcctgacgcc gttcagccgc ctggtaacgc
    35761 cgaaaattac cgagatcctg ggctttaact taccagtcga tatgatagcg acctcccacg
    35821 gcgtggtctg gcgcgataac ccgacgcaaa ttgtcgagct gtacctgaag tgggccgctg
    35881 attatcagga agacagaatc accattttct acgacaccat gtcgaataac acccgcatga
    35941 tggctgacgc tatcgcccag ggaattgcgg aaaccgaccc acgcgtggcg gtgaaaattt
    36001 tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg
    36061 tgctggtcgg cacttcgacg atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg
    36121 aggagatgac cggattacgc ttccgtaaca aacgcgccag tgctttcgga tctcacggct
    36181 ggagcggcgg tgcgatggat cgcctttcca cgcgcctgca ggatgcgggt ttcgaaatgt
    36241 cgctcagcct gaaagccaaa tggcgaccag accaggacgc tctggagtta tgccgtgaac
    36301 acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct gccgcagagc acggtgaata
    36361 cggtagttaa agaagaaacc tctgccacga cgacggctga cctcggccca cgaatgcagt
    36421 gcagcgtctg ccagtggatt tacgatccgg caaaaggcga gccaatgcag gacgttgcgc
    36481 caggaacgcc gtggagtgaa gtcccggata acttcctctg cccggaatgc tccctcggca
    36541 aagacgtctt tgacgaactg gcatcggagg caaaatgagc aacggcattg tgatcatcgg
    36601 ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc aaacaggacg ccactattcc
    36661 attaaccctg attgccgccg acagcataga tgagtacaac aaacccgacc tcagccatgt
    36721 tatcagtcag gggcaacgtg ccgacgacct tacccgccag acggcgggtg aatttgccgg
    36781 gcagtttaat ctgcgcctgt ttccgcacac ctgggtgacg gatatcgatg ccgaagccca
    36841 tgtggtgaaa agccagaata atcagtggca atacgacaag ttagtgctgg caaccggtgc
    36901 cagcgccttt gtcccgcctg tgcctgggcg tgagttaatg ctgacgttaa atagtcagca
    36961 agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga cgcgtgttga ttgtcggcgg
    37021 tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct
    37081 aatcgacaac gctgccagta ttctggcgtc attaatgcca ccagaagtaa gcagccgctt
    37141 gcagcatcgg ttgacggaga tgggcgttca tctgctgttg aaatctcagt tgcaggggct
    37201 ggaaaaaacg gattctggca ttctggcaac gctggaccac cagcgtagta tcgaagtgga
    37261 tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc ctggcacgac gcgccgggct
    37321 gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa accagtaatg ccgatattta
    37381 cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg ccgttcctgc agccgattca
    37441 acttagcggg atggtgctgg caaaaaatct tctcggcaat aacacgccgc tgaaactacc
    37501 ggcgatgctg gtgaaaatca aaacgccgga attaccgctg catctggcag gcgaaaccca
    37561 gcgtcaggat ttacgctggc agattaatac cgaacgccag ggaatggtgg cgcgcggcgt
    37621 tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag gatcggatga aagaggcatt
    37681 tgtattgttg aaaacgttgc cggtgtaggt gggctactgt gcctaaaatg tcggatgcga
    37741 cgctggcgcg tcttatccga cctacgggga cgcatgtgta ggccggataa ggcatttacg
    37801 ccgcatccgg caagggtgtc caaatgcaac acgctttatc cgttctggac ttcacccgct
    37861 aaccaacgcg ccgcagcaat aaccccctgc cccagagaca aaccgccatc acccgccggt
    37921 aaactctgtg gaaagagcaa tgtgaaatca gcgagataat gcgccagacg tgcacgcagc
    37981 aaacggttat gaataacccc gccgctaaat accagcgtag tgataccacg catcgtggcc
    38041 tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt
    38101 tgattaacca gtgcctgcca gttcagccac tgctgccaga aagtggcgag atccagttga
    38161 ttgtccaccc gcggcatcgt caccggatgc gtcactccgt cgcatgaggc tgcgagcgcc
    38221 tccagagcac aagccgcttc accttcataa cttaacgtgg ctggcgcaca gcccagtgcc
    38281 gccgccactg catcgaacaa ccgcccacac gatgatgcca gcggcgcgtt aattccacgc
    38341 tcaatggccc gcgccagtac gctccagttt tgctgttgca cacttgccgt ttcagggtaa
    38401 ttctgccact ccggcacaaa gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc
    38461 gccgccaaat cgccacccgg aagcgccact gcaggcaagc cgcccaggtg ctcacattcg
    38521 cgatagttca cccgcaggca ctcgccgccc cacaaagcgc cattctcccc cataccgata
    38581 ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg gccactgatg ctctgccaga
    38641 cacgccgctg catgggcatg atgatgcagt accgtttgcg tcggcagatt catttcgctc
    38701 gcccactggc tggagacata gcccggatgc acatcatgca caatgtattg cggagtgaaa
    38761 tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact gcatctggat gccatcgtca
    38821 cttaaatcgc ccagatgctg actcaacacc gcttgttcac cgcgcaccag gcagaaggta
    38881 tttttcagat ccgcgccaag acacagcaca ggcggaacat ttttaaagcc cggaggcaaa
    38941 accagcgcat ccggcacata cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc
    39001 actgaatcat ccatccgctg cacgatgtcg cggttatgta gcaagaatcc gtcggcaatg
    39061 ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg gtggtttacc gctcaggttg
    39121 ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc
    39181 gcaggcaaca tcaccccgac ttcgttaagg ccaggggcga tatcatcaca aagctcaggt
    39241 acgtattttt tatccaccag cacaatcggc gcagcgggcg tggtaagcaa ctggcgcgca
    39301 gcgtctggta aactttctgc cactggcaac atgactgcca gcggtttcgc cgggcgatgt
    39361 ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg catcgcaggc aagatgaaat
    39421 ccgccaatcc ctttgatggc gacaatgttg cccattttta actgtgcgat agctgcctgt
    39481 aatgccgcct cttgttctgc atgttcacca tgacttaccc attcaagatg cgggccacac
    39541 tccgggcagg ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg gtactctttg
    39601 tcacaggccg gacatagcgg aaacgccgcc atcacggtaa acgggcggtc gtaaggcatc
    39661 gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt tgataaacgg ataacgataa
    39721 cgccgctcgc ctggggtatt catttcggca aggcaagcag ggcaagtggc ggcatccggg
    39781 acaatttgcg tattcatggt gccgccggtg ctctggcgga tggtgaactc ggtgggcagt
    39841 tgtgaccaga taaacggctc acgctcgacg ctatcaatac gcgccagtgg cggacagtgc
    39901 tgatgcaatt gaacaagaaa cgtttccggg tcttccagca gccggacttc tacgccatcg
    39961 ccgtcattac atacatcgcc gtgaagattt aattgctgtg ccagctgcca gacaaacgga
    40021 cgaaaaccga cgccctgcac tttgccacga atacgcagtt ggacaccgca agatgtgttt
    40081 tttgccattg agttattccc gccatcatga attgcgtaac ccgccctgcc ggacacgaca
    40141 gcgtcgtatc cggcagtctc aggtcggcga tcccgcccgc tccgtattct acgaatattt
    40201 ccgggaattc ctttgatgcc agaacagttc tgtaagattt ttagaacatc agcgccgtac
    40261 ggcggcgttt ttctgcgctc agttgttcaa gtttattacg atcgacacaa atcagcgcat
    40321 gggtcgggca agccgccata cacgccgggc cgtcttcacg atggttgcac aggtcgcatt
    40381 tattggcttc ggctttgtca gcccgtacat tcagacccgc gccgctgttg cgaatcacag
    40441 gacgtaccac cacttccatc gcgccatacg ggcaagccac cacgcaggtt ttgcaaccaa
    40501 tgcaacgttc ctgcatcaca ggaacaaacc ctttatcacg gctgatagca ccattcgggc
    40561 agacgttagc gcacggtgca tcttcacact gacggcaaac tgtcgccgtg gaaatgttca
    40621 cacctttaat gacatggata cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct
    40681 gattttcctg atgagaaacc acgcacgcca cttcacaggt acggcaacca atacatttac
    40741 tcgcgtcagc aatgatgaaa cggttcatca aattctccag caatgacagt taatgcgccg
    40801 atacattcac aaatcatgcc agtttttaat ttgctgttat ttaaggaaat taatttctgt
    40861 aatgcaggaa aaaagatgtc atcgacacta gtgacgatga catgtgatga caatgtttat
    40921 cgcgaaaggg caatgagtga gtcgcggcgg atcagttttc cgctaaaggt tttcggcggt
    40981 gagaaatccc cgccatcgag cataaaaatc agccgtccaa taatttcctg aatcatctca
    41041 gtcaccggaa tttttacgct ggagagcgcc ggaacgatat agggggcaat tgcgatatca
    41101 tcgaatccga taactgacac ctgctctggc accgctacgc cgcgctcgtg caacgctttg
    41161 atcgcaccta tcgccatatc gtcgttactg gcaactaacg cgctaaattt agcccctcgt
    41221 tcgagcaacg tttctacccc ttcggccccg ctggcgggcg tccatttacc gttagcgata
    41281 agtttttcat tgagcgcaat accatgctgc gacagcgcgt ctttataccc ggcaagacgt
    41341 tcaatgctgg tgggggaatc catcgagccg gtaaggaaag caatctcctg atgcccggcg
    41401 tttatcaact ctgccacggc gttaaagctg gtctgtttat gatcgcacca gacgctatgg
    41461 ctgctattat tgcgcaggcg gcgattaagc accattatcg gctgactgtg cgcgtcaatg
    41521 atgtcatcaa tctcatccac gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc
    41581 aaatccagca gatactgaat cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc
    41641 aatagcaact gtcgcccttt ctcttccgcc attcgcgcgg catgaaacag taattcacta
    41701 aaataaatgc cgtgataaag cgtgttggtc actaccagcc ccagcgtctg agtactcttc
    41761 gccgacagat tgcgggccag caaatttggg cggtaactgc tctcttctac cgcctgaaac
    41821 acgcgatctt tagtctcctg gctgacgtag ccattacctg aaagcacgcg ggaaacggtc
    41881 gcttttgaaa ccccggcgcg cttcgccact tccagcatcg tcgtcatcat tttcatccct
    41941 ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat tgtccttgcg caatcggctc
    42001 cagcggataa gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat
    42061 ggcgattcct tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt
    42121 gtacggcacc actttgctct tcttcttacg cacatcgact acggtcatgc ggtcggtaca
    42181 ggagtagcaa gggtccaggc taccgataat cagcggcgca tcggaaacgg tattgccacg
    42241 cagcatgtag cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta
    42301 cagcttctgg ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc
    42361 aaagcccagc gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc
    42421 cggcaggtta tccagaccgt agtcgatcat attcagcgcg gtatagacct cgttgattcg
    42481 cactttcaga cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag
    42541 gccataaccg acaaacgggt gatcggcgcg agtatcgcgg gcgagaccgc tggcgcgaac
    42601 catcgggcca acgttactga aatcgcgggc gatttccggg tccagacgac caatgccgac
    42661 ggtgcgttgt tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg
    42721 acgcatttgt tgcgccagct ggcgggtctg gatcatgtca tctttcagca gatcgcgacg
    42781 aatcccgccg atcaggttca ggccgtaggt tttacgcgca ccggtaagga tctctgccat
    42841 tttcatggag gtttcacgca cgcggaagaa ctgcataaag ccggagtcga agccggtaaa
    42901 gtgacaggcg aggccgaggt tgagcagatg cgggtgcagg cgttccacct ccagcagaat
    42961 ggcgcggatc atctgcgcgc gttctggcac ctgaataccc atcgcgtttt ccaccgacgt
    43021 ggtgtaggcg gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt
    43081 cacttcgtta taacccatgc gggtttccgc cagtttttcc ataccgcgat ggacgtagaa
    43141 cagacggtag tcggcgtcga taatgttttc gccatcgacg aacagacgga agtgacccgg
    43201 ttcgtcagaa gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag
    43261 ttcgttgatg aactcgtagg tttcagcatc ggtagtcggt gccggacgct gacgataatc
    43321 catgctgtct ttacgcagcg gataaagttc atccggccag tcatccggca gcaccagacg
    43381 acgttcatcc ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc
    43441 ccacaccgcc gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcgtcaac
    43501 ttcgacgcga accgttaccc aacacttggt gcccttctcc atcgacagca cgtaataaac
    43561 ggcgtaatga ccattcagtt tgcgttcgtc gttaccaaac agcaccgaca gccagccacc
    43621 ctgcttgtag tagagaaact ccaccacttc cggcagatag ttcaccttta cggtgacagt
    43681 cagctgatct ttggtctgcc aggcgtggtc cagcacgacg cctagaaatg cctcattcag
    43741 cgcggcgaga taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac
    43801 gccgccagca aggagacgaa cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc
    43861 ggagtaatat caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg
    43921 ccgaccacca gtttgacgat ggcaatcacc agcgccagca gcagtccacc gacggtgaag
    43981 gtttccattt gtccccacgg aataaacacc ccgacgaaca tctgcaacac caccagctgt
    44041 ttcaggctga taccccattt catgacgcca aagccgctac cgctgtattc ggagagcgga
    44101 ccttcctgca actcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata
    44161 aaagtggcga acgcacaggc gcagagcgcc agcaccagcg ggatgctttg gctcagcggc
    44221 cagtgataaa cggtgtcggt aatgttgctg atgttggtgg aaccggcaac ctgtgcggca
    44281 acccacagac caagcagcaa catcggttcg accagcacgc caagcatcgc ttcacggctc
    44341 gcaccgatag cggtaaacgg gctaccggta tccagaccgg aaatggcaaa gaagaaacgc
    44401 gcgatggcga agagatacag taaggtgatc aaatcgccca gttgcggcag cggagaaccg
    44461 acggtcacca ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc
    44521 gtcaggcgga acatccagcc agaggcatcc gggccaacgc tctggcggcc cagcagtttg
    44581 ataatgtcgc gatactcctg caacacgccc ggcccgcgac ggttatgcag acgggcgcgc
    44641 gccacgcggg taataccgga gagcagcggc gcaacggcaa ataacaccag cgcctgaatt
    44701 aacggatata aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagtaccg
    44761 ccagttcaac cagcgccatc cggcggaaca gcaacgcact accttcgcac tgccagcccg
    44821 gcactagcga caccggattc agccatttgc gcagtttcag cactggcgca aacgcctgtt
    44881 taaccggcat ggcaaaaccg tgagcggtaa tcaccattga tttttcatgg tcgtagccgc
    44941 acacccaggc cgcaccgcgg gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa
    45001 tgatgaatgg cagcagcggg caggcaatca gcagcaatgt gatcattggt tgagaaacgg
    45061 tggtgttagc aggctccaga ggcagaggta cagcagcaga gagcatcggc agcagccacg
    45121 gcgcagcaac accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca
    45181 ggagcggcgc acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga
    45241 cgccatagac tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag
    45301 cgagcagcgg ccccagcaga cgggcaacaa acgcgccgct attgctcagt ttgaagaagg
    45361 attgatagat aacccattcc ccggcaaacc cgttcagtgg cggcagcgca ccgctcacgc
    45421 caaccgcgcc agtcagtaca gtgaagcccg cggctgccgt atacagacta ccaaccgcgc
    45481 cgccaattcc ggctatccag ccacttagcg ctttttgaaa agagaaatgc cagaacagcg
    45541 gcggcgacaa accatgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc
    45601 cactttgagc ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac
    45661 gcttacgttt gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg
    45721 ggcacatccg cacacaggcc ggaccttgtt catcaaagct acaaaggtca catttgacgg
    45781 cgatcgcgcg aatacctggc acccagtcaa gcaatgtgct gacacgcgcc ggagcaggcg
    45841 gtgccggtgg cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg
    45901 aaaattcaat tgcgccaaac gggcaggcga tgccgcacag tttgcagctt acgcacagac
    45961 tttcattcaa ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg cagaccaccg
    46021 cgcagggtgc atcttcacag tggtgacaga gctgcggcgc agattctttt tcattgagca
    46081 tcactctcag gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg
    46141 cctcacaggt gtggcagccg atacagagcg tggagtcggc aattacaaaa cgattcacca
    46201 ggcattcctc aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga
    46261 cactcatcga cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca
    46321 gctcatgctg ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt
    46381 tcaatggcgg tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga
    46441 acctgttcgc gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgc
    46501 gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg
    46561 tggctgttaa agccatccgc cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa
    46621 cagagttctt tgtccggcgc gcagctcatg gcgtgataca ggcgcgcttt cgataacgtg
    46681 atcccctgaa ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc
    46741 tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttactcgtt
    46801 taacagagat gccagcttta agcatttttt gtgccaactt ttaatttatt gttattaaag
    46861 agatttttaa gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg
    46921 acattattca ccgcagggat aatcaacact ggcgcaatta ttgcttgtag ctggcaatag
    46981 ttaatgggag gcgatatgca cgaaataacc ctctgccaac gggcactgga attgatcgaa
    47041 cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca
    47101 ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc
    47161 gtggcggaag gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca
    47221 tgccaacagt atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt
    47281 gacatgctac agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag
    47341 gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca acctgtatat cgagggtgat
    47401 gaacataacc ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgcag
    47461 atgaaaatca ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac
    47521 ctgcattacg gtcatggcga agcgggcact cacacaccgg gcatgagcca gcgtcggatg
    47581 ctggaagtcg aaattgacgt gctggacaaa aacaaccgtc tggctgaacg caaccgcgcg
    47641 cgctttgctg cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa
    47701 accaccctgc tgacggaaac cttaatgcgc ctgaaagaca gcgttccgtg cgcggttatt
    47761 gaaggcgacc agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg tacaccagcg
    47821 attcaggtga acaccggtaa aggctgccat cttgacgcac agatgattgc cgacgccgca
    47881 ccgcgtctgc cactggacga taacggtatt ctgtttatcg aaaacgttgg caacctcgta
    47941 tgcccggcca gcttcgatct cggtgaaaaa cacaaagtag cggtgctttc cgttaccgaa
    48001 ggtgaagaca aaccgctgaa atatccgcat atgtttgccg ccgcctcgct gatgctgctc
    48061 aacaaagttg acctgttgcc gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc
    48121 cgcgaagtca atccagaaat tgaaatcatc cttatttccg ccaccagcgg cgaagggatg
    48181 gaccagtggc tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc
    48241 gcaccattga cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc gatgtcgatt
    48301 taacgttagt cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg
    48361 ttcacgttgg ctttgccatg agcgtaatta atgaagccga agcacgcgac actctcgacg
    48421 ccttacaaaa catgtttgac gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa
    48481 aataatgcgt tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca
    48541 tctgcgcgaa cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga
    48601 agtgtgtggc ggtcataccc acgccatttt taaattcggc ctcgaccagt tactgcctga
    48661 aaacgttgag tttatccacg gtccgggttg cccggtgtgc gtactgccga tgggtagaat
    48721 cgacacctgc gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc
    48781 catgcgcgta ccagggaaac agggatcgct gttgcaggca aaagcacgcg gtgccgatgt
    48841 gcgcatcgtt tactcgccga tggatgcgtt gaaactggcg caggagaatc caacccgcaa
    48901 agtggtgttc ttcggcttag gttttgaaac caccatgccg accaccgcca tcactctgca
    48961 acaggcgaaa gcgcgtgatg tgcagaattt ttacttcttc tgccagcaca ttacgcttat
    49021 cccgacgctg cgcagtttgc tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc
    49081 gggccacgtt agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca
    49141 tcgtccgctg gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct
    49201 ggtggagcag aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc
    49261 ggatgccggt aacctgctgg cgcaacaggc gattgccgat gtgttctgtg tcaacggcga
    49321 cagcgaatgg cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta
    49381 tcaacgattc gatgccgaag cacatttccg cccggcaccg cagcaggtct gcgatgaccc
    49441 gcgcgcgcgt tgtggtgagg tattaacggg caaatgtaag ccgcatcaat gcccgctgtt
    49501 tggtaacacc tgtaatcctc aaaccgcatt tggtgcactg atggtctcct ccgaaggagc
    49561 gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa gcgtgaataa tatccaactc
    49621 gcccacggca gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc
    49681 tttgccaacc cgtggctggc agagcaggaa gatcaggcac gtcttgatct ggcgcagctg
    49741 gtagcggaag gcgaccgtct ggcgttctcc accgacagtt acgtcattga cccgctgttc
    49801 tttcctggcg gtaatatcgg caagctggcg atttgcggca ccgcgaatga cgttgcgggc
    49861 agtggcgcta ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg
    49921 gagacactga aagccgtagt gaccagcatg gcagaaaccg cccgcacggc aggcattgcc
    49981 atcgttactg gcgatactaa agtggtgcag cgcggcgcgg cagataaact gtttatcaac
    50041 accgctggca tgggcgcaat tccggcgaat attcactggg gcgcacagac gctaaccgca
    50101 ggcgatgtat tgctggttag cggtacactc ggcgaccacg gggcgactat ccttaacctg
    50161 cgtgagcagc tggggctgga tggcgaactg gtcagcgact gcgcggtgct gacgccgctt
    50221 attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt
    50281 gtaaacgcgg tggttcatga gttcgcggca gcctgcggtt gtggtattga aatttctgaa
    50341 tcagcgctgc cggttaaacc tgccgtgcgc ggcgtttgcg aattgctggg actggacgcc
    50401 ctgaactttg ccaacgaagg caaactggtg atcgccgttg aacgcaacgc ggcagagcaa
    50461 gtgctggcag cgttacattc ccatccactg gggaaagacg cggcgctgat tggtgaagtg
    50521 gtggaacgta aaggtgttcg tcttgccggt ctgtatggcg tgaaacgaac cctcgattta
    50581 ccacacgccg aaccgcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt
    50641 caatgacctt ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc
    50701 gatgagtgat ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc
    50761 cgatctggcc tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga
    50821 caacgcggct attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcgtcgcg
    50881 tgaaaaagac acccccatta aatatgaaga cgaaactgtt ctggcacacg gtccggtacg
    50941 cagcattttg tcgcgccctg atacgctgca ttgcagttac gaagaatttt gtgaaacctg
    51001 gccgcagctg gccgcaggtg ggctataccc caaatttggt cactattgcc tgatgccact
    51061 ggcggcggga gggcatattt ttggtggctg tgaatttatt cgttatgacg agcgtccctg
    51121 gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag atcgtttctg tcgtcaccga
    51181 acaaatccag agccgcgtcg tcaacaatgt cgactatgag ttgttatgcc gggagcgcga
    51241 taacttccgc atcctggttg ccattaccaa cgcggtgctt tcccgcctgg atatggacga
    51301 actggtcagc gaagtcgcca aagaaatcca ttactatttc gacattgacg atatcagcat
    51361 tgtcttacgc agccaccgta aaaacaaact caacatctac tccactcact atcttgataa
    51421 acaacatccc gcccacgaac agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt
    51481 gttcaaaagt aaagagatgc tgctgatcaa tctctacgag cgggacgatt tagcccccta
    51541 tgaacgcatg ttgttcgaca cctggggcaa ccagattcaa accttgtgcc tgttaccgct
    51601 gatgtctggc gacaccatgc tgggcgtgct gaaactggcg caatgcgaag agaaagtgtt
    51661 taccactacc aatctgaatt tgctgcgcca gattgccgaa cgtgtggcaa tcgctgtcga
    51721 taacgccctc gcctatcagg aaatccatcg tctgaaagaa cggctggttg atgaaaacct
    51781 cgccctgacc gagcagctca acaatgttga tagtgaattt ggcgagatta ttggccgcag
    51841 cgaagccatg tacagcgtgc ttaaacaagt tgaaatggtg gcgcaaagtg acagtaccgt
    51901 gctgatcctc ggtgaaactg gctcgggtaa agagctgatt gcccgtgcga tccataatct
    51961 cagtgggcgt aataatcgcc gcatggtcaa aatgaactgc gcggcgatgc ctgccggatt
    52021 gctggaaagc gatctgtttg gtcatgagcg tggggctttt accggtgcca gcgcccagcg
    52081 cattggtcgt tttgaactgg cggataaaag ctctctgttc ctcgacgaag tgggcgatat
    52141 gccactggag ttacagccga agttgctgcg tgtattgcag gaacaggagt ttgaacgtct
    52201 cggcagcaac aaaatcattc agacggacgt acgtttaatt gccgcgacta accgcgatct
    52261 gaaaaaaatg gtcgccgacc gagagttccg tagcgatctc tattaccgcc tgaacgtatt
    52321 cccgattcac ctgccgccac tacgcgagcg tccggaagat attccgctgc tggcgaaagc
    52381 ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc gacagcattc ctgccgagac
    52441 gctgcgcacc ttgagcaaca tggagtggcc gggtaacgta cgcgaactgg aaaacgtcat
    52501 tgagcgcgca gtattgctaa cacgcggcaa cgtgctgcag ctgtcattgc cagatattgc
    52561 tttaccggag cctgaaacgc cgcctgccgc aacggttgtc gcccaggagg gcgaagatga
    52621 atatcagttg attgtgcgcg tgctgaaaga aaccaacagc gtggttgccg ggcctaaagg
    52681 cgctgcgcaa cgtctggggc tgaaacgcac gaccctgctg tcacggatga agcggctggg
    52741 aattgataaa tcggcattga tttaactgca aattgccgga cagatctgcc tgtccggcat
    52801 actattcacg aggttttttc ggacgatatt tttccggcag ttctggcacc ggacgcttgt
    52861 catcgatgag atgacgcacg gttaagatcg gatgacgcca cagcattctc ggcccggccc
    52921 aacgcataat ctgtttcatc tcttcacgct ttgcaggctg gtaacagtgc accggacact
    52981 gcttacaggc tggtttctct tcgccgaaca cacatttatc cagccgcttt tgcgcgtaaa
    53041 caaacaacgc ctcgtaatgc tccggctccg ctgacgcctg cgggcatttc gcttgataaa
    53101 gatcgatcat ttttttaatc gtcagttttt cacgagagat acgcttgccg gacatgctgc
    53161 ctccacctca ttaagatgca tttatattac atcttaatc
//
LOCUS       DAEEDS010000020        52078 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEEDS010000020 DAEEDS010000000
VERSION     DAEEDS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52078
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.020"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KLH32_001257"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KLH32_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBE0138158.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="KLH32_001258"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="KLH32_001258"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE0138159.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="KLH32_001259"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="KLH32_001259"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE0138160.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="KLH32_001260"
     CDS             complement(5906..6949)
                     /locus_tag="KLH32_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBE0138161.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KLH32_001261"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KLH32_001261"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBE0138162.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KLH32_001262"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KLH32_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBE0138163.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="KLH32_001263"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="KLH32_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="KLH32_001264"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="KLH32_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBE0138164.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KLH32_001265"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KLH32_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBE0138165.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="KLH32_001266"
     CDS             15474..15830
                     /locus_tag="KLH32_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBE0138166.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="KLH32_001267"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="KLH32_001267"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBE0138167.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="KLH32_001268"
     CDS             16989..17189
                     /locus_tag="KLH32_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBE0138168.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KLH32_001269"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KLH32_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0138169.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KLH32_001270"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KLH32_001270"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBE0138170.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="KLH32_001271"
     CDS             complement(20101..20964)
                     /locus_tag="KLH32_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBE0138171.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KLH32_001272"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KLH32_001272"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBE0138172.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KLH32_001273"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KLH32_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBE0138173.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDVGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KLH32_001274"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KLH32_001274"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBE0138174.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="KLH32_001275"
     CDS             24133..24705
                     /locus_tag="KLH32_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBE0138175.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="KLH32_001276"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="KLH32_001276"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBE0138176.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="KLH32_001277"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="KLH32_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBE0138177.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="KLH32_001278"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="KLH32_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="KLH32_001279"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="KLH32_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBE0138178.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="KLH32_001280"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="KLH32_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBE0138179.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="KLH32_001281"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="KLH32_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBE0138180.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="KLH32_001282"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="KLH32_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBE0138181.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="KLH32_001283"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="KLH32_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="KLH32_001284"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="KLH32_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBE0138182.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="KLH32_001285"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="KLH32_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBE0138183.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="KLH32_001286"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="KLH32_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBE0138184.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="KLH32_001287"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="KLH32_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBE0138185.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="KLH32_001288"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="KLH32_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="KLH32_001289"
     CDS             complement(39099..40160)
                     /locus_tag="KLH32_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE0138186.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="KLH32_001290"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="KLH32_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBE0138187.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="KLH32_001291"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="KLH32_001291"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBE0138188.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KLH32_001292"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KLH32_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBE0138189.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KLH32_001293"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KLH32_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBE0138190.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KLH32_001294"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KLH32_001294"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBE0138191.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="KLH32_001295"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="KLH32_001295"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBE0138192.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="KLH32_001296"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="KLH32_001296"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBE0138193.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVQAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="KLH32_001297"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="KLH32_001297"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBE0138194.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="KLH32_001298"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="KLH32_001298"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBE0138195.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52078)
                     /gene="pdeF"
                     /locus_tag="KLH32_001299"
     CDS             complement(50969..>52078)
                     /gene="pdeF"
                     /locus_tag="KLH32_001299"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBE0138196.1"
                     /translation="YKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALD
                     SHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQR
                     CGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDEL
                     IGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFP
                     VEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYAS
                     YARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIRE
                     AVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12160 a        13738 c        13519 g        12661 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 caacatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggagct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacag gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgta
//
LOCUS       DAEEDS010000021        50696 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEEDS010000021 DAEEDS010000000
VERSION     DAEEDS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50696
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.021"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..1146
                     /locus_tag="KLH32_001300"
                     /pseudo
     CDS             1..1146
                     /locus_tag="KLH32_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1248..1400
                     /gene="hokA"
                     /locus_tag="KLH32_001301"
     CDS             1248..1400
                     /gene="hokA"
                     /locus_tag="KLH32_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBE0138197.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1587..1799)
                     /gene="cspA"
                     /locus_tag="KLH32_001302"
     CDS             complement(1587..1799)
                     /gene="cspA"
                     /locus_tag="KLH32_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBE0138198.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(2080..2370)
                     /locus_tag="KLH32_001303"
                     /pseudo
     CDS             complement(2080..2370)
                     /locus_tag="KLH32_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2804..3514
                     /locus_tag="KLH32_001304"
     CDS             2804..3514
                     /locus_tag="KLH32_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBE0138199.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3564..4538)
                     /gene="ghrB"
                     /locus_tag="KLH32_001305"
     CDS             complement(3564..4538)
                     /gene="ghrB"
                     /locus_tag="KLH32_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBE0138200.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4642..5292)
                     /locus_tag="KLH32_001306"
     CDS             complement(4642..5292)
                     /locus_tag="KLH32_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBE0138201.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5455..7788
                     /gene="bisC"
                     /locus_tag="KLH32_001307"
     CDS             5455..7788
                     /gene="bisC"
                     /locus_tag="KLH32_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBE0138202.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7757..8197)
                     /locus_tag="KLH32_001308"
     CDS             complement(7757..8197)
                     /locus_tag="KLH32_001308"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0138203.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(8194..8757)
                     /gene="tag"
                     /locus_tag="KLH32_001309"
     CDS             complement(8194..8757)
                     /gene="tag"
                     /locus_tag="KLH32_001309"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBE0138204.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8915..9613
                     /locus_tag="KLH32_001310"
     CDS             8915..9613
                     /locus_tag="KLH32_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0138205.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9842..11050
                     /locus_tag="KLH32_001311"
     CDS             9842..11050
                     /locus_tag="KLH32_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0138206.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            11374..13065
                     /gene="eptB"
                     /locus_tag="KLH32_001312"
     CDS             11374..13065
                     /gene="eptB"
                     /locus_tag="KLH32_001312"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBE0138207.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            13157..13233
                     /locus_tag="KLH32_001313"
     tRNA            13157..13233
                     /locus_tag="KLH32_001313"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13191..13193,aa:Pro,seq:cgg)
     gene            13977..15584
                     /gene="dppA"
                     /locus_tag="KLH32_001314"
     CDS             13977..15584
                     /gene="dppA"
                     /locus_tag="KLH32_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBE0138208.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15892..16911
                     /gene="dppB"
                     /locus_tag="KLH32_001315"
     CDS             15892..16911
                     /gene="dppB"
                     /locus_tag="KLH32_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBE0138209.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16921..17823
                     /gene="dppC"
                     /locus_tag="KLH32_001316"
     CDS             16921..17823
                     /gene="dppC"
                     /locus_tag="KLH32_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBE0138210.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17834..18817
                     /gene="dppD"
                     /locus_tag="KLH32_001317"
     CDS             17834..18817
                     /gene="dppD"
                     /locus_tag="KLH32_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBE0138211.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18814..19818
                     /gene="dppF"
                     /locus_tag="KLH32_001318"
     CDS             18814..19818
                     /gene="dppF"
                     /locus_tag="KLH32_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBE0138212.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19848..21119)
                     /locus_tag="KLH32_001319"
     CDS             complement(19848..21119)
                     /locus_tag="KLH32_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE0138213.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            21595..21702
                     /locus_tag="KLH32_001320"
     CDS             21595..21702
                     /locus_tag="KLH32_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0138214.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22078..22185
                     /gene="ldrD"
                     /locus_tag="KLH32_001321"
     CDS             22078..22185
                     /gene="ldrD"
                     /locus_tag="KLH32_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBE0138215.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22561..22668
                     /locus_tag="KLH32_001322"
     CDS             22561..22668
                     /locus_tag="KLH32_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0138216.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            23044..23151
                     /locus_tag="KLH32_001323"
     CDS             23044..23151
                     /locus_tag="KLH32_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0138217.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(23238..24917)
                     /gene="bcsG"
                     /locus_tag="KLH32_001324"
     CDS             complement(23238..24917)
                     /gene="bcsG"
                     /locus_tag="KLH32_001324"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBE0138218.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(24914..25105)
                     /gene="bcsF"
                     /locus_tag="KLH32_001325"
     CDS             complement(24914..25105)
                     /gene="bcsF"
                     /locus_tag="KLH32_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBE0138219.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(25102..26673)
                     /gene="bcsE"
                     /locus_tag="KLH32_001326"
     CDS             complement(25102..26673)
                     /gene="bcsE"
                     /locus_tag="KLH32_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBE0138220.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            26946..27134
                     /locus_tag="KLH32_001327"
     CDS             26946..27134
                     /locus_tag="KLH32_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBE0138221.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            27146..27898
                     /gene="bcsQ"
                     /locus_tag="KLH32_001328"
     CDS             27146..27898
                     /gene="bcsQ"
                     /locus_tag="KLH32_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBE0138222.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            27895..28254
                     /locus_tag="KLH32_001329"
     CDS             27895..28254
                     /locus_tag="KLH32_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBE0138223.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            28251..30512
                     /gene="bcsA"
                     /locus_tag="KLH32_001330"
                     /pseudo
     CDS             28251..30512
                     /gene="bcsA"
                     /locus_tag="KLH32_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            30523..32862
                     /gene="bcsB"
                     /locus_tag="KLH32_001331"
     CDS             30523..32862
                     /gene="bcsB"
                     /locus_tag="KLH32_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBE0138224.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            32869..33975
                     /gene="bcsZ"
                     /locus_tag="KLH32_001332"
     CDS             32869..33975
                     /gene="bcsZ"
                     /locus_tag="KLH32_001332"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBE0138225.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            34034..34210
                     /locus_tag="KLH32_001333"
                     /pseudo
     CDS             34034..34210
                     /locus_tag="KLH32_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            34538..36286
                     /gene="bcsC"
                     /locus_tag="KLH32_001334"
                     /pseudo
     CDS             34538..36286
                     /gene="bcsC"
                     /locus_tag="KLH32_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            36368..38356
                     /gene="hmsP"
                     /locus_tag="KLH32_001335"
     CDS             36368..38356
                     /gene="hmsP"
                     /locus_tag="KLH32_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBE0138226.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            38542..39828
                     /gene="dctA"
                     /locus_tag="KLH32_001336"
     CDS             38542..39828
                     /gene="dctA"
                     /locus_tag="KLH32_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBE0138227.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            40049..41545
                     /locus_tag="KLH32_001337"
     CDS             40049..41545
                     /locus_tag="KLH32_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE0138228.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(41641..42570)
                     /gene="kdgK"
                     /locus_tag="KLH32_001338"
     CDS             complement(41641..42570)
                     /gene="kdgK"
                     /locus_tag="KLH32_001338"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBE0138229.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            42802..43569
                     /gene="pdeH"
                     /locus_tag="KLH32_001339"
     CDS             42802..43569
                     /gene="pdeH"
                     /locus_tag="KLH32_001339"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBE0138230.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            43639..45699
                     /locus_tag="KLH32_001340"
     CDS             43639..45699
                     /locus_tag="KLH32_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE0138231.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(45881..47203)
                     /locus_tag="KLH32_001341"
     CDS             complement(45881..47203)
                     /locus_tag="KLH32_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBE0138232.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(47614..48627)
                     /gene="yhjD"
                     /locus_tag="KLH32_001342"
     CDS             complement(47614..48627)
                     /gene="yhjD"
                     /locus_tag="KLH32_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBE0138233.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(48676..49575)
                     /locus_tag="KLH32_001343"
     CDS             complement(48676..49575)
                     /locus_tag="KLH32_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0138234.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            50095..>50696
                     /locus_tag="KLH32_001344"
     CDS             50095..>50696
                     /locus_tag="KLH32_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0138235.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        12210 a        12608 c        13661 g        12217 t
ORIGIN      
        1 gatcgagcag cacatgtccc ttaatcaggc tgctgctcac tttatgcttg ctggtagtgg
       61 ttctgtagcc aggtggctga aggtctatga agagcgcgga gaagctggtt tacgcgcgct
      121 caagattagc accaaaagaa acattgcaat atcagttgat ccagaaaaag cggcatcagc
      181 attggagctg tcaaaagacc gacgcattga ggatcttgaa aggcaagttc gatttcttga
      241 aacgcggctt atgtatctaa aaaagctgaa agccttagct catcccacga aaaagtgaaa
      301 gtactcaacg agctaaggca gttttatcct cttgatgagc ttctcagggc tgcggagata
      361 ccgcgcagta cgttttatta ccatctaaag gctctcagca agcctgacaa gtatgcggac
      421 gttaaaaagc gtattggtga gatttatcac gagaatagag gccgatacgg ataccgtagg
      481 gtaacgctgt ctcttcatcg agaagggaaa cagattaacc ataaagctgt tcagcgcctg
      541 atgggaaccc tctcacttaa agcagcgatt aaggtcaagc gataccgctc ttacagagga
      601 gaggtagggc aaaccgcccc taatgttctc caaagagatt tcaaggctac gcggccaaac
      661 gagaagtggg ttaccgatgt tactgaattt gcagtcaatg ggcgcaagct gtatttgtct
      721 ccagtaatag atctcttcaa caacgaagtt atttcttaca gcctttcgga aagaccagtg
      781 atgaacatgg ttgagaatat gctcgatcag gcattcaaaa agcttaatcc tcacgagcat
      841 cctgttctgc actctgacca gggatggcag tatcgtatga gaagatatca aaatatcctt
      901 aaagaacatg gtattaaaca aagcatgtcc agaaaaggca attgtctgga taatgctgtg
      961 gtggagtgtt tctttggaac cttaaagtcg gagtgttttt atcttgatga gttcagtaat
     1021 ataagcgaac tgaaggatgc tgttacggaa tatattgaat actataacag cagaagaatt
     1081 agcctgaaat taaaaggtct gactccaatt gaatatcgga atcagaccta tatgcctcgt
     1141 gtttaactgt ccaacttttt ggggtcagta caaagtgagg ctaccctatg cctgaacacc
     1201 ataaggttag cctcttactc gttggaaatc aacacagggg gctgggaatg ccgcagaaat
     1261 atagattact ttctttaata gtgatttgtt tcacgctttt atttttcacc tggatgataa
     1321 gagattcact gtgtgaattg catattaaac aggagagtta tgagctggcg gcgtttttag
     1381 cctacaaatt gaaagagtaa gagtcttcgg cgggaaatta ttcccgcctt acttacggcg
     1441 ttgcgcattc tcattgcacc catatttatt cttcacaaaa ataataatag attttattac
     1501 gcgatcgatt atttatttcc tgaaaaaaaa taaaaaaatc cccgccaaat ggcagggatc
     1561 ttagattctg tgcttttaag cagagattac aggctggtta cgttaccagc tgccgggcct
     1621 ttagcgccgc tttcgatggt gaaggacact ttctgacctt cgtccagaga tttgtaacca
     1681 tcgttctgga tagcagagaa gtgtacgaac acatctttag agccatcgtc aggagtgatg
     1741 aagccgaagc ctttgtcagc gttgaaccat tttacgatac cagtcatttt accggacata
     1801 gtgtattacc tttaataatt aagtgtgcct ttcggcgata tggcgtgctt tacagatttt
     1861 gaagcgttaa aggaatgtgc actacgaggg gtatcaacga taactcttga agggacttgc
     1921 cttactacac tgctttaatg gtctgtacgt caaaccgttg atgtgcatta agccactcat
     1981 tggcgggtga tgcaacaatt atttttcata tttatgatta atcggttgga aaacggtcct
     2041 gtcatcagga ccgtaaacag caataaagtg gataaaagtc tatttcatca actgcttact
     2101 taatgccggg ttggcttgaa tcaaacgcat caattttagt tcggcacttg aaggcttcac
     2161 gcgtctggat tcccattcct ttaccatggc gactgatacg cccaaaaccc gggcgaaatc
     2221 atcgattttt aatcctgtcc cttttcataa ctgctcaatt tcggtacagg acgttgttct
     2281 gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca atctgttcca ggctgctcaa
     2341 cagctcatgc attggatctt tatattccat tgaaaactcc tcaaatcaca ctgcgggatc
     2401 gtgaattgca tcgaagctca ttaagaatag tcgggaaaag cagacacagc ggggtgaagt
     2461 cagggcatgc tctggctatg attcgattca gcctgtcatt tagcgtgact tatagccacc
     2521 taacgatctg ataaatgccg atatatctct tatcactcct gttttgtaaa gatttcgcaa
     2581 atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa aaggcaacgg cactatcatt
     2641 ttttaacaat gaaacaacaa attaaccttg tttcattttc tccatgattt taccaggaag
     2701 attacgtaga aagggtatcc tgcaagcctg tcctggacta tccttgtcac gtcagacacg
     2761 cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag aaaatggcga caggaaagtc
     2821 ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg gtagttagcc tgagtgggtg
     2881 ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac ttcctgcaaa atacagtgat
     2941 gcgtagcggt gaacgtctac caaccctgac tgccgatcag aaaaaacagt ttggtccttt
     3001 tgtctctgat tacgcgattc tgtatggtta ttctcagcag gtgaatcagg cgatggattc
     3061 cggtctgcgc ccggtcgtag acagcgttaa cgcaattcgc gtaccgcagg attacgttac
     3121 gcaaagcggt ccgctgcgtg aaatgaatgg ttcgctgggt gtgctggcgc aacaactgca
     3181 aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta aaacagagcg atgacctgaa
     3241 accggtattt gatcaggcat tcaccaaagt ggtaacgacg ccagctgatg cattgcaacc
     3301 gttgattccg gcggcacaaa ctttcacgca acaactggtg atggttggag actacattgc
     3361 tcagcaaggt actcaggtga gctttgtcgc aaatggcatc cagttcccga cttcacagca
     3421 ggcaagtgaa tataacaaac tgattgcgcc attaccggca caacatcagg cgtttaatca
     3481 ggcctggact acagcagtta ctgcaaccca ataaagagta aaagcccgag cgattatcgg
     3541 gctttacttc acgcagtcgc agcttagtcc gcgacgtgag gattcacaca gttcttctca
     3601 acctttcctt gtaacgcatc aatcaaatta tccacggcac aggcggccat gccataacgc
     3661 gtctcatggg tggcagatcc aatatgcggt actgcgacga cgttggccat tgagagcaac
     3721 ggcgaatcta cggacagtgg ctcttgttcg aagacatcca gcccggcggc gtgaatttcg
     3781 cctttctgca atgctgcgat cagtgcattt tcgtcaacca ccgggccacg tccggcatta
     3841 atgaaaatgg cggaggattt cattttggcg aattgttctg cgccaaacag atgatgcgtc
     3901 tcatcagtta acggcaggat caggcaaacg aaatctgact cttgtaacag agtatccaaa
     3961 tcgcagtagc gggcgttgaa gcgttcttct gcttctttat ggtggcggcg cgcgttatag
     4021 aggatgggca tgttgaagcc aaagtgcaca cgttgtgcca gcgccatgcc gatccgtccc
     4081 atcccgacaa tgcccagtgt tttatggtga acgtcagtgc cgtaccagtc cgggcctatg
     4141 ctcgcggtcc attcgcctgc ttttacccgt tctgctacct ccacaacccg acgagcggta
     4201 gacaacacca gcgccatcag cgtatcggcg acggtttctg ttaatacggt tggcgtgtgc
     4261 atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa aattgtcata gccgacggag
     4321 atcgttgatg tggcacgcag tttcggcatt ttttccagca atgcggcatc tacattctcg
     4381 tttgaaccca gtaaaccttc agcttctgca aaaattgctg cattttgaac gacggtttgt
     4441 gggctgaggt ttgccacctg gtgaacggtg aaatgcgctt gcaggcgttg cagtaaatca
     4501 tcaggtaagg ctttgtagag gataacggac ggcttcatgc ttctctccat tcactgataa
     4561 ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga agaatgccag gtgatttctg
     4621 catcacctgg catgaaaggg attacagcgg gcttaaggta atttctacac ggcggttttg
     4681 cgccttacct tctgcggtgc tgttgctggc gattgggtta gccgggccaa ggccctgagt
     4741 acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg ctggcaacgg aatccgcacg
     4801 ctgctgggag agacgcatgt tcaggtcgtg accccccgtg ctgtcggtat aaccaatcac
     4861 gttaaccgcc gttttcggat actctttcag caccattgcc acgccggtca gggtgttagc
     4921 gcctgccggt ttcaggggag cgctgctgct gtcgaaggtc acattgttcg gcatattgag
     4981 gataatgtta tccccgctgc gggttacgct aacaccagtg ccgcgcattt tgtcacgcag
     5041 cttcgcttcc tgcacatcca tgtaataacc cacgccgccg cccagagctg cgcctgctgc
     5101 tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa gaagagagcg caccaatacc
     5161 cgcgcccacg agagagccca gacctgcgcc gatagcagat ttgcctgctt cgcgttcgcc
     5221 ggtgtaaggg ttagttgtgc agccagatac cgccagagcg ccactcacta cggcggcaag
     5281 taagataaac acgtttcttc attgttaatc cttaataacc tttttattct ttgtcacggg
     5341 ttccgtggcg ggagattatg ccgcgtgaac atgaagatta ttcctgggaa tactcggaaa
     5401 tttgtaagta atatttaact gctcaataca tctaaccttt caggagtctt cggtttggcc
     5461 aactcatcct cacgatattc cgttctgact gccgcccact gggggcccat gctggttgaa
     5521 accgacggcg aaaccgtgtt tagctcgcgt ggcgcgttag ccacaggaat ggaaaactcc
     5581 ttgcagagcg cggttcgcga ccaggttcac agcaatacgc gggtacgatt tccaatggtg
     5641 cgcaaaggct ttctcgcgtc accggaaaac ccgcaaggca ttcgtgggca ggatgaattt
     5701 gttcgcgtga gttgggatga ggcgctggat cttattcacc aacaacataa acgcattcgt
     5761 gaggcttatg gtccggcatc gatttttgct ggttcctacg gctggcgttc aaacggcgtg
     5821 ctgcataagg cctcgacatt attacaacgc tatatggcgc tggcaggcgg ttataccggg
     5881 catctggggg attattcgac cggtgcggca caggcgatca tgccgtatgt cgtaggtggc
     5941 agcgaagttt atcaacagca aaccagttgg ccgctggtgc tggaacatag cgatgtcgtg
     6001 gtgttgtgga gtgctaaccc actcaatacg ctgaaaattg cgtggaatgc atccgatgag
     6061 caggggcttt cttacttttc cgcactgcgt gacagtggga aaaagctgat ctgcattgat
     6121 ccaatgcgat cggaaaccgt cgatttcttt ggcgataaaa tggagtgggt ggcaccgcac
     6181 atgggcaccg atgttgcgct gatgctgggg atcgcccata cgctggtgga aaatggttgg
     6241 cacgacgaag cgtttctggc gcgttgcacc acaggttatg ccgtcttcgc ctcttatttg
     6301 ctgggcgaga gtgacggaat agcgaaaaac gccgaatggg cggcagagat ttgtggtgtt
     6361 ggcgcagcga aaatccgcga gctggcggct attttccacc aaaataccac catgctgatg
     6421 gcaggctggg gaatgcagcg ccaacagttt ggtgagcaaa aacactggat gatcgtcacg
     6481 ctggcagcaa tgttggggca aatcggcaca cccggcggcg gttttggtct ttcttaccat
     6541 tttgccaatg gtggtaaccc cacgcggcgt tctgcggtgc tctcttccat gcagggcagc
     6601 ttgccgggtg gctgcgatgc ggtggataaa atccctgttg cccgcattgt tgaagcactg
     6661 gaaaaccctg gtggcgcata tcaacacaac ggtatgaacc gacatttccc ggatattcgt
     6721 tttatctggt gggcgggcgg tgccaacttt actcatcatc aggataccaa tcgtctgatc
     6781 cgtgcctggc aaaaaccgga gctggtggtg atctctgaat gcttctggac ggcggcggcg
     6841 aaacacgcgg atattgttct gcctgcgact acctcttttg agcgtaatga tctcaccatg
     6901 accggtgatt acagtaatca gcatctggtg ccgatgaagc aagtggtgcc gccacgctat
     6961 gaagcgcgta atgattttga tgtttttgcc gagttgagtg aacgttggga gaagggcggc
     7021 tatgcgcggt ttacggaagg aaaaagtcag ctgcaatggc tggaaacgtt ttataacgtt
     7081 gcccggcagc gcggggcaag ccagcaggtt gaattgccgc catttgctga gttctggcaa
     7141 gccaaccagt taattgagat gccggaaaac ccggacagcg agcggtttat tcgcttcgct
     7201 gatttttgcc gcgatccgct ggcgcatccg ttaaaaaccg ccagcggcaa gattgaaatc
     7261 ttctcacagc gtattgccga ttacggttac ccggattgcc ctgggcatcc aatgtggctg
     7321 gagccggacg aatggcaggg caatgccgaa ccagaacagt tgcaggtact ttctgcccat
     7381 ccggcgcacc gcctgcacag ccagctgaat tacagttctc tgcgcgaatt gtacgcggta
     7441 gcaaaccgtg agcctgtcac cattcatcct gacgatgctc aggcgcgcgg cataaccgaa
     7501 ggggatatgg tgcgggtgtg gaactcccgt gggcagatcc ttgccggagc ggtcattagc
     7561 gagggaatta aacctggcgt gatttgcatt cacgaagggg catggccgga tctggattta
     7621 accgctgacg gtatttgtaa aaacggcgca gtgaacgtgc tgaccaaaga tctccccagc
     7681 tcgcggctgg ggaatggctg tgcgggtaat acgacgctgg catggctgga aaaatacaac
     7741 ggtccggaac tgacacttac agcgtttgaa ccaccggcca gctcataatc caggtgggta
     7801 gttgggtttc atcctgccat gcgcaatcga caatgtgaaa accctgcgca cggtaaaaat
     7861 ctatcgccgg ttgatttttt tgataaacct ccagcatcag atggggatag cgctgctgca
     7921 catactgcat cagcgcctta ccaataccgc gtctgacggc cttcggtgcg acaaacattg
     7981 ctgccagaaa tcggccttcc ataatgctga caaaaccgag aagcttaccg tcctcttccc
     8041 agacccagtt ttgcgcgttg gcaagatagg catcccgcac cagcggaatg cagtcacgcc
     8101 agtaattcgc ttttataaag ggatgccccc aggttgtact ttccagccac agttcgagga
     8161 tcgcggggag ttctgaacgt tgcgcttccc gaatcatggt ttatttccca gatagcaaca
     8221 gcagccaacc acatgatcat tcaccagccc acatgcctgc ataaaggagt aacagattgt
     8281 ggtgccgaca aacttaaaac cacgtttttt cagtgcctta gatagggcgt cggaggcgga
     8341 cgtagatgtg ggaatttcgc tcaacgttgt ggcttgtgtc acctgtggct gatgatttac
     8401 aaacgaccag acaaagtcgg gaaacggttc gccgttctgt tccatttgca ggtacgcccg
     8461 cgcattacca ataattgcct gaattttccc tcgatggcgg ataatcccgg cgtcctgtac
     8521 cagtctttcg acatcctctt cctgcattgc tgcgaccttc accggatcga actgatgaaa
     8581 gcaggcgcga tagttttcgc gttttttgag gacggtgatc cacgataacc cagcctgctg
     8641 cccttcaaag cagatcattt cgaacagttt tttactgtca gtttcaggca cgccccactc
     8701 attatcatgg taggcaatat aaagcgggcc ctgactcacc cagccgcaac gttccatact
     8761 ttccctcgct atgtacccga tcgtaaaaat ttcagccgct tttcttgacg catcggtgaa
     8821 aaagtcaata tttaatacag ggctactatg cccgatatcc ttcatacaat gacaataata
     8881 ttgccgaatt cggctcttcc ctggagtcgt gttaatgatc ataaaaaaaa gcggtggtcg
     8941 ctggcagcta agcctgctgg cgagcgtggt aatcagtgcc ttttttctca acacagctta
     9001 cgcctggcaa caagaatata tcgttgatac gcaacccgga cattccacag agcgttacac
     9061 ctgggatagt gatcatcaac ctgattacaa cgatattttg tcgcaacgta ttcaaagtag
     9121 ccaaagggcg ctgggactgg aagtcaatct ggcggaagaa actcctgtgg atgtgaccag
     9181 cagtatgagt atgggctgga attttccttt atatgaacag gttacaaccg gcccggtcgc
     9241 ggcattacat tacgatggca caaccacctc gatgtataac gagtttggcg acagtactac
     9301 cacgctgacc gatccgttat ggcatgccag cgtgagtagc ttaggctggc gtgttgactc
     9361 ccggcttggc gatctccgac cctgggcgca aatcagctat aaccagcaat ttggcgagaa
     9421 tatctggaag gcgcaatcag gcctgagccg gatgacggcg acaaaccaga acggcaactg
     9481 gctggatgtc accgtaggcg ctgatatgtt gctcaatcaa aatattgccg cctatgctgc
     9541 gctaactcag gcagaaaata ccactaataa tagcgactat ctgtatacga tgggggttag
     9601 cgccagattt taacgtaaca gtcacaattg aaaccattga ataacaacag ttgtggcgat
     9661 agtgggtgct aacttaccaa ataataaatt tggtgaataa ttgtcgcgtc attcattcct
     9721 gaactaaggc atttcattcc gttctgatgg catttcatgc cgtttttccc caagcataaa
     9781 atgcacttcg ttatggttgt cggcagagat ttttcctttt tattactgca ggaatactgc
     9841 catgacacct tcaaattatc agcgtacccg ctggctgaca ctcatcggta ctatcattac
     9901 ccagtttgcg ctggggtcgg tttatacctg gagcctgttt aatggcgcgc tttctgccaa
     9961 gctggatgcg ccggtaagcc aggtcgcttt ctctttcggc ttgttaagtc tggggctggc
    10021 aatttcgtct tctgttgcgg gcaaattgca ggaacgtttt ggcgttaaac gcgtcaccgt
    10081 ggcttccggc attttgctgg gattaggctt cttcctgacc gcgcattcca acaacctgat
    10141 gatgctgtgg ttaagcgccg gtgtgctggt cggtctggcg gatggcgcgg gttacctgct
    10201 gacgctctct aactgcgtga agtggttccc ggagcgtaaa ggtctgatct ccgcgttcgc
    10261 tatcggttct tatggtctgg gtagcctggg tttcaaattt atcgacacgc agctgctcga
    10321 aacggtgggt ctggaaaaaa cctttgtgat ttggggagcg attgcgctgg tgatgattgt
    10381 tttcggcgca acgttaatga aagacgcacc aaaacaggaa gtgaaaacca gcaatggtgt
    10441 ggtggagaaa gattacacgc tggcagagtc gatgcgtaaa ccgcagtact ggatgttagc
    10501 ggtaatgttc ctgactgcct gcatgagcgg cctgtacgtg attggggtag cgaaagatat
    10561 cgcccaaagt ctggcacacc ttgatgtggt ttccgcagcc aatgcagtaa cggttatttc
    10621 catcgctaac ctttcaggtc gtctggtgct gggcattctg tctgacaaaa ttgcccgtat
    10681 ccgtgttatc accattggtc aggtgatttc gctggtgggt atggcggccc tgctgtttgc
    10741 accattaaat gcagtgacgt tctttgcagc gattgcctgc gtggcattta actttggcgg
    10801 cactattacc gtctttccat cactggtcag tgagttcttc ggcctcaata acctggcgaa
    10861 aaactacggt gtgatttacc tcggtttcgg tatcggtagc atttttggtt cgattatcgc
    10921 ctcactgttt ggcggtttct atgtgacctt ctacgtgatt ttcgccctgc tgattctgtc
    10981 attggcgctt tctacgacga ttcgtcagcc agagcagaaa atgttgcgtg aggcgcatgg
    11041 ctccctttaa tgctcagccg cgagctactg ggcttcagct tccgtgtgca tcaaacgcat
    11101 caacgaaatg tataaacaat gactcgtaaa atttgggcca gttaacaggc cctttttact
    11161 gctttttgta acgctaaagt ctcttttcaa acttgcattt ttgtaaattt gtgcttcatg
    11221 cacactcttt ccccacactt tttcgctttg ctgtggtcta cttattcgcg cgtgtagatt
    11281 ttacttatct gactacctcc gcacttttcc cctgccgggc ctgaaaagcc gctaagcagg
    11341 gtgttatcac ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc gattacacag
    11401 cagaagctga gctttttgct tgcaatctat attggccttt ttatgaatgg cgcggttttt
    11461 tatcgccgct tcggcagcta tgcgcacgat tttaccgtct ggaaaggcgt ttctgctgtt
    11521 gttgaactgg ccgccaccgt actggtgacc ttctttttac tacgtcttct atcgctgttt
    11581 ggccgccgca gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc aggtgccagc
    11641 tattacatga ccttccttaa tgtggtcatt ggttatggca tcatcgcttc cgtcatgacc
    11701 accgatatcg acctgtcaaa agaagttgtt ggtctgaact ttattctctg gttaatcgcc
    11761 gttagtgcat tgcctcttat ccttatctgg aataaccgct gtcgctacac cttgctccga
    11821 caactgcgaa ccccagggca gcgtattcgc agcctggtgg tcgtcgtact ggcgggtatt
    11881 atggtttggg caccgattcg tttgctggat atccagcaga agaaagtgga gagggcgacc
    11941 ggcgttgatt tgccgagtta tggcggtgtc gtagcgaact cttatctgcc atcaaactgg
    12001 ctttctgcgt tggggctgta tgcctgggcg cgggtcgatg aatcttccga taataattca
    12061 ttgcttaatc cggcgaagaa gtttacatac caggctccac aaaacgttga tgatacttat
    12121 gtcgtgttta tcatcggtga aaccacgcgt tgggaccata tgggtatttt cggctatgag
    12181 cgtaatacca cgccgaaact ggcccaggag aaaaatctgg cggcgttccg tggttactcc
    12241 tgtgataccg caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg gggcgcggaa
    12301 gataatccgc agcgcacatt aaaagaacag aacattttcg cggttctgaa gcagttagga
    12361 ttcagttctg acctctacgc tatgcagagc gaaatgtggt tctacagcaa cacgatggca
    12421 gacaacattg cttatcgtga gcagattggt gcggagccac gtaatcgtgg caagccggta
    12481 gatgatatgt tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc ggatggtaag
    12541 catctgatca ttctgcatac caaaggttcg cattttaact acacccagcg ttatccgcgt
    12601 agcttcgcgc agtggaagcc ggaatgtatt ggtgttgata gcggctgtac caaagcgcag
    12661 atgatcaact cctatgacaa ctcggtgacc tatgtggatc actttatctc cagcgtgatt
    12721 gatcaggttc gcgataagaa agcgattgtg ttctacgcag ctgaccacgg tgagtcaatt
    12781 aatgaacgcg agcacctgca cggcacgccg cgtgaactgg caccgccgga gcagttccgc
    12841 gtaccgatga tggtctggat gtcagataaa tatctggaaa atccggccaa tgcgcaggcg
    12901 tttgcgcagc tgaaaaaaga agccgacatg aaagtgccac gccgtcacgt agagctgtac
    12961 gacaccatca tgggttgtct tggctatact tcaccggatg gtggaattaa cgaaaacaac
    13021 aactggtgtc acatcccgca gacaaaagtg gcagcggcta actaagcggc ctgctgactt
    13081 tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta
    13141 agatgcgccc cgcattcggt gattggcgca gcctggtagc gcacttcgtt cgggacgaag
    13201 gggtcggagg ttcgaatcct ctatcaccga ccaaattcga aaagcctgct caacgagcag
    13261 gtttttttgc atttgtagct catgaggatg agaacctccg ggggcaggga ggctcgactc
    13321 gacgtaggcc tgataagacg cgccagcgtc gcatcaggca acggcaccgc acttattgtc
    13381 ggatgcggcg cgaccgcctt attcgaccaa cacaccacac attttcctgc caactttgcg
    13441 ccccatatct cactatccat taacggattt gtgacagaaa ccattgtctt tttttatatt
    13501 tgcttaaatc ttttttgcgt tacttatggc tggcgctggc attttccgct gtgcgtttta
    13561 gcgttcatgg tattaatcgc tcagatatcc accttttcct cagatttttt tcattaaatg
    13621 cataaaactt aatcaccaaa tgttgctaaa tattaagctg attgttctgg ttatgttgga
    13681 gtagcacaaa tttctctgct gcaaatagct gtttgaagtt tttttaatct ttgtttgtgg
    13741 attctcaccg ttggtgtaaa tcccggttgg ctgtattgac gttttcacat tctgttgaca
    13801 gattgtaggt cacgaggggc attttatgga ggatccgcac tgttacactg atgttaatta
    13861 gtacggcatc cccacctcat aacgttgacc cgaccgggca aaaaacaaaa aaggtcaggc
    13921 agcgacaacc cactgcaaag ggttaaaaca acaaacatca caattggagc agaataatgc
    13981 gtatttcctt gaaaaagtca gggatgctga agcttggtct cagcctggtg gctatgaccg
    14041 tcgcagcaag tgttcaggct aaaactctgg tttattgctc agaaggatct ccggaagggt
    14101 ttaacccgca gctgtttacc tccggcacca cctatgacgc ctcttccgtc ccgctttata
    14161 accgcctggt tgaatttaaa atcggcacca ccgaagtgat cccgggcctc gctgaaaagt
    14221 gggaagtcag cgaagacggt aaaacctata ccttccatct gcgtaaaggt gtgaagtggc
    14281 acgacaataa agaattcaaa ccgacgcgcg aaatgaacgc cgatgacgtg gtgttctcgt
    14341 tcgatcgtca gaaaaacgcg caaaacccgt accataaagt ttctggcggc agctacgaat
    14401 acttcgaagg tatgggcttg ccggagctga tcagcgaagt gaaaaaggtg gacgataaca
    14461 ccgttcagtt tgtgctgact cgaccggaag cgccgttcct cgctgacctg gcaatggact
    14521 tcgcctctat tctgtcaaaa gaatatgctg acgcgatgat gaaagccggt acaccggaaa
    14581 aactggacct caacccaatc ggaaccggtc cgttccagtt acagcagtac caaaaagatt
    14641 cccgtatccg ctacaaagcg tttgatggct actggggcac caaaccgcag atcgatacgc
    14701 tggtcttctc tattacccct gatgcttccg tgcgttacgc gaaattgcag aagaatgaat
    14761 gccaggtgat gccgtacccg aacccggcag acatcgctcg catgaagcag gataaatcca
    14821 tcaacctgat ggaaatgccg gggctgaacg tcggctacct ctcgtataac gtgcagaaaa
    14881 aaccgctgga tgacgtgaaa gttcgccaag cactaaccta cgcggtgaac aaagacgcga
    14941 tcatcaaagc ggtttatcag ggcgcgggtg tatcagcgaa aaacctgatc ccgccaacca
    15001 tgtggggcta taacgacgac gttcaggatt acacctacga tcctgaaaaa gcgaaagcct
    15061 tgctgaaaga agcgggtctg gaaaaaggtt tctccatcga cctgtgggcg atgccggtac
    15121 aacgtccgta taacccgaac gctcgccgca tggcggagat gattcaggca gactgggcga
    15181 aagtgggcgt gcaggccaaa atcgtcacct acgaatgggg tgagtacctc aagcgtgcga
    15241 aagatggcga acatcagacg gtaatgatgg gctggactgg cgataacggg gacccggata
    15301 acttcttcgc caccctgttc agctgcgccg cctctgaaca aggttctaac tactcaaaat
    15361 ggtgctacaa accgtttgaa gatctgattc aaccggcgcg tgctaccgac gaccataaca
    15421 aacgcgttga actgtacaag caagcgcagg tggtgatgca cgatcaggct ccggcactga
    15481 tcatcgctca ctccaccgtg tttgaaccgg tacgcaaaga agtcaaaggc tatgtggttg
    15541 atccattagg caaacatcac ttcgaaaacg tctctatcga ataattaaaa gccatacaag
    15601 actgatggca aaggcaaaac tgcctgatgt gctccgctta tcaggcctac gaaaattctg
    15661 caatgtattg aatttgcacg attttgtagg ccggataagg cgttaacgcc gcatccggca
    15721 taaacaaagc gcactttgtc aacaatctgt atacccggtg gcgctgtgcc tctgcgtgca
    15781 gcgccatccg gcagcaatac ttcattctct gtccgacaag ggcgggaatt gatttgtgag
    15841 caatacagac acgcagttcc aggctgcgag tcactataga gaatccgggt tatgttgcag
    15901 tttattctcc gacgtttggg actcgtcatc cccacgttta tcggtattac ccttctcaca
    15961 tttgcctttg tccacatgat cccgggcgat ccggtgatga tcatggcagg cgaacgtggg
    16021 atctcccctg agcgtcacgt gcagctgctg gctgaactcg gcttagataa accgatgtgg
    16081 cagcagtatc tccattacat ttggggcgta atgcacggcg atctaggcat ttcaatgaaa
    16141 agccgaattc cagtatggga agagttcgtg ccgcgctttc aggccacgct ggaacttggc
    16201 gtctgcgcga tgatttttgc tacggcagtc ggtattccgg ttggtgtgct ggctgcggtt
    16261 aaacgcggtt ccattttcga tcacactgca gttggcctgg cgctgaccgg ctactcgatg
    16321 ccgatcttct ggtggggcat gatgctgatc atgctggttt cggtgcactg gaacctgacg
    16381 cccgtctccg gtcgcgtgag cgatatggtg ttcctcgatg actccaatcc gttaaccggt
    16441 tttatgctaa tcgacaccgc catctggggt gaagacggca actttatcga tgccgtcgcc
    16501 catatgatct tgcccgccat tgtgctgggt actattccgc tagcggtcat tgtgcgtatg
    16561 acacgctcct cgatgctgga agtgctgggc gaggattaca tccgcaccgc gcgcgccaaa
    16621 gggctaaccc gcatgcgggt gattatcgtc catgcgctgc gtaacgcgat gctgccggta
    16681 gtgaccgtta tcggcctcca ggtgggaaca ttgctggcgg gggcgattct gaccgaaacc
    16741 atcttctcgt ggcccggtct ggggcgctgg ttgattgacg cactgcaacg ccgcgactat
    16801 ccggtagtgc agggcggcgt attgctggtg gcgacgatga ttatcctcgt caacttgctg
    16861 gtcgatctgc tgtacggcgt ggtgaacccg cgtattcgtc ataagaagta aggggacatc
    16921 atgtcacagg ttactgaaaa taaagtgatt agcgcaccgg tgccgatgac cccgttacag
    16981 gagttctggc actattttaa acgcaacaaa ggcgcggtcg tcgggctggt ttacgtcgcc
    17041 atcgtgctgt tcatcgcgat ctttgccaac tggattgcgc cttataaccc ggcggatcag
    17101 ttccgcgatg cgctgctcgc cccgccagcc tggcaggaag gcggcagtat ggcacacttg
    17161 ctgggcaccg atgacgtcgg tcgtgatgtg ctgtcgcggc tgatgtacgg cgcgcgttta
    17221 tcgctgctgg ttggctgtct ggtggtggtg ttgtcgctga ttatgggcgt tattctcggc
    17281 ctgatcgccg gttacttcgg cggcctggtt gataacatta tcatgcgcgt ggtcgatatc
    17341 atgctggcgc tgccaagcct gctgttggca ctggtgctgg tggcgatttt cggcccgtcg
    17401 attggtaacg ccgcgctggc gctgaccttc gttgccttgc cgcactatgt gcgcttaacc
    17461 cgcgccgccg tgctggtgga ggtgaatcgc gattacgtca ccgcctctcg cgtggcaggt
    17521 gccggggcga tgcgccagat gtttattaac atcttcccga actgccttgc gccgctgatt
    17581 gttcaggcgt cgctcggttt ctctaacgcc attctcgata tggctgctct tggtttcctc
    17641 ggcatggggg cacagccgcc aacgcctgag tggggcacta tgctctccga cgtgttgcag
    17701 ttcgcgcaaa gcgcctggtg ggtcgtgacc ttccctggtc tggcgatcct gttgacggtg
    17761 ctggcattta acctgatggg tgacggtctg cgtgacgcgc tcgatcccaa actaaagcag
    17821 taagaggttc gagatggcgt tattaaatgt agataaatta tcggtgcatt tcggcgacga
    17881 aagcgcaccg ttccgcgccg tagaccgcat cagctacagc gtaaaacagg gcgaagtggt
    17941 cgggattgtg ggtgagtccg gctccggtaa gtcggtcagt tcactggcga ttatggggct
    18001 gattgattat ccgggccgcg taatggcaga aaaactggag tttaacggcc aggatttgca
    18061 gcgtatctca gaaaaagagc gccgcaacct ggtgggtgcc gaagtggcga tgatcttcca
    18121 ggacccgatg accagcctta acccgtgcta caccgtgggt ttccagatta tggaagcgat
    18181 taaagtgcat cagggcggca acaaaagtac ccgccgtcag cgggcgattg acctgctgaa
    18241 tcaggtcggt attcccgatc cggcttcgcg tctggatgtt tacccgcatc agctttccgg
    18301 cggcatgagc cagcgcgtga tgatcgccat ggcgattgcc tgtcggccaa aactgctgat
    18361 tgccgatgaa ccgaccaccg cgctggacgt gaccattcag gcgcaaatca tcgaactact
    18421 gctggagcta cagcagaaag agaacatggc gctggtgtta attacccatg acctggcgct
    18481 ggtggcggaa gctgcgcata aaatcattgt gatgtatgca ggtcaggtag tagaaacggg
    18541 cgatgcacac gccatcttcc atgcgccgcg tcacccgtat actcaggcat tgctgcgtgc
    18601 gctgccagaa tttgctcagg acaaagaacg tctggcgtcg ttgccaggtg ttgttcccgg
    18661 caagtatgac cgcccgaacg gctgcctgct taacccgcgc tgcccctatg ccactgacag
    18721 atgtcgcgct gaagaaccgg cgctgaatat gctcgctgat gggcgtcagt ccaaatgcca
    18781 ttacccactt gatgatgccg ggaggcccac actatgagta cgcaagaggc cacctcgcaa
    18841 caaccgctgt tgcaggctat cgacctgaaa aaacattatc cggtgaagaa aggcatgttc
    18901 gtgccggaac gtctggtgaa ggcgctggac ggcgtttcgt ttaaccttga acgtggcaaa
    18961 acgctggcag tagtgggcga atctggctgc ggtaaatcga ccctcggtcg gttgctgacg
    19021 atgatcgaaa cgcccaccgg cggcgagctg tattatcagg ggcaggatct gctcaagcac
    19081 gatccgcagg cgcagaagct gcgtcggcag aaaatccaga tcgtcttcca gaatccctac
    19141 ggttcgctga atccgcgtaa gaaagttggg caaattctcg aagagccgct gcttattaac
    19201 accagcttaa gcaaagaaca gcgtcgggaa aaagccctgt cgatgatggc aaaagtcggc
    19261 ctgaaaaccg agcactatga ccgctatccg cacatgtttt ccggcggaca acgtcagcgt
    19321 atcgctattg cccgtggtct gatgctcgac ccggatgtgg tgattgccga tgagccggtt
    19381 tctgcgctgg acgtctcagt gcgtgcgcag gtgctgaatc tgatgatgga tttgcagcag
    19441 gagttggggc tttcttatgt ctttatctcc cacgacctgt cggttgtgga gcacattgct
    19501 gatgaagtga tggtgatgta cctaggccgc tgcgtggaga aaggaacgaa ggaccaaatc
    19561 ttcaataacc cgcgccatcc gtacacccag gcgctgctct ccgcgacgcc acgcctgaac
    19621 ccggacgatc gtcgtgaacg catcaaactc accggtgaac tgccaagccc gctgaatcct
    19681 ccgccgggtt gcgccttcaa cgcccgctgt cgtcggcgct tcggcccctg cacccagttg
    19741 cagccgcagc taaaagacta cggaggtcaa ctggtagctt gttttgctgt tgatcaggat
    19801 gaaaatccgc agcgttaact cacaaaaccg gggccatgcg ctccggttta ctcaatcagc
    19861 ttcaacagtg gcgacaagca tagcaaaatg ccgtaaagca gaatcagcca ggctttaaat
    19921 ccgcgaagtt tttccagttg tgcgacttta tagatcagga aaaacggaat gaggcacgac
    19981 acaatgccat ataacgggct tcccaactga aagaacacca gcactgatac acgaaacgaa
    20041 acccaaatcg tcaacgtaat gacgataaaa gcgcagatcg ccagagtcag catgcgtgag
    20101 ttaattttct tggtatcaat aattcggctt aacagattga gaataatgcc tttaatagcc
    20161 tcgtggaaac cgagataaat gccaaagaat gcggtcagta cggcaaagat attcagcacc
    20221 gtagaggtga tatgaatgat atgtccaggg atcacctgcg cggccagtgc cagtgccgag
    20281 atattttgtt caaaggcaga aacggcttct tcgtggctaa ttgagaaggt aaacgaaaag
    20341 gcaaaaaaca ggatcaccgc gatgagcgtg atataactaa ttcggtgggt acgcagcgcg
    20401 agccgggttg ccagtacttt atctgcttcc cgtttacgat aggcaatatt cattgggtta
    20461 agtacctgaa taaataccgc agaaaagaag caaaatggaa tggtaagcag gacatcgcgg
    20521 aaaaagacgg aggcttgcgg gaaggcggtt atattggcga aattccagtg cgggatcatc
    20581 gcaaaaccga acacgacaat aatccctact ttgaccacca ccattggccc agaaatctta
    20641 aacagtaatc gttcaccacc agacgcaatc gctatcagta cggcgaaaat agcgacttta
    20701 taaagtagag attgtgaaag atcggcatcg gttaaaccga aggttttcag gtacgaggcg
    20761 ctgtcgaaaa ccacggagag agagtagata aaaatcccgt ggataatcat caaaaagtag
    20821 ataaccccga ggaaaattcc ccagttcttc cccaggtaat gactgataat atcggtgtag
    20881 tcattacagg aatcgctttc agaaagggtt tttaaataaa tgtcctgcac tacccaggtg
    20941 acaggataag caatgatcgc tgcggtaata aatacccaaa ttcccttcaa gccaatttgt
    21001 actggcatca gcacggttcc ggcaccaatc gccatgccta tgcataataa aacccagccg
    21061 aagtcgtagc gtgtaaacgg caatttctga ttgtgtttcg atagtgtgtt gtgctgcata
    21121 aagtcctgtc cagaaagaag tcggcctcgc aaaaatgaat aaacccagtt tctcttgcgc
    21181 aacaggtgga gaagtcgaat acgcagtatg taaaaaatga ggcaacagat tcactgggga
    21241 ggtcgcgagg aggcgcaatt ctacaaaatt gcgcatttca ctaccagtct ggagtcagcc
    21301 cggatatgaa ctgcaactat tgcaaataaa gaaattaagc tggaaggcat cgcgcaatga
    21361 ggaagattgc cgggtgctga cagtaaaaag caatcgtaga atcagcgtca agccggaagg
    21421 attccggtga ggcgcaatgt tgcgggggct taatccctgg tggcattggt tgctggagag
    21481 agaaaacccc cgcacgttgc aggtatgtac ctgacaacat aacgggggct aatcttgaac
    21541 ctagaccact caagaatagc cgcgaaacgt tgtcattaca acacaggcgg ctatatgacg
    21601 ctcgcagagc tgggcatggc cttctggcat gatttagcgg ctccggtcat tgctggcatt
    21661 cttgccagta tgatcgtgaa ctggctgaac aagcggaagt agcgtgtcat gtgggcgtca
    21721 ggctgccgta atggcaattt gcgcccggac caggccgcag gggtgaaact ctgcggcctt
    21781 tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt ctgcggaatg cgcagcacag
    21841 agtgaagatg agacggattc cggaacagaa aaacagtcgt agaatcagcg tcaagccgga
    21901 aggattccgg tgaggcgcaa tgttgcgggg gcttaatccc tggtggcatt ggttgctgga
    21961 gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa caccacgggg gccattcttg
    22021 catccaaaca atacaagaat agccgcgaaa cgttgtcatt acaatacagg cggctatatg
    22081 acgttcgcag aactgggcat ggccttctgg catgatttag cggctccggt cattgctggt
    22141 attcttgcca gtatgatcgt gaactggctg aacaagcgga agtaacgtgc catgcgggcg
    22201 tcaggctgcc gtaatggcaa tttgcgcccg gaccaggccg caggggtgaa actctgcggc
    22261 ctttttcgta agaaaaatgc aacggttgca agcggataat attctgcgga atgcgcagca
    22321 cagagtgaag acgtgacgga ttccggaaca gaaaaacagt cgtagaatca gcgtcaagcc
    22381 ggaagggttc cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct
    22441 ggagagagaa aacccccgca cgttgcaggt atgcacctga caacatcacg ggggccattc
    22501 ttgaacctga acaactcaag aatagccgcg aaacgttgtc attacaacac aggcggctat
    22561 atgacgctcg cagaactggg catggccttc tggcatgatt tagcggctcc ggtcattgct
    22621 ggcattcttg ccagtatgat cgtgaactgg ctgaacaagc ggaagtaacg tgtcatgcgg
    22681 gcgtcaggct gccgtaatgg caatttgcgc ccggaccagg ccgcaggggt gaaactctgc
    22741 ggcctttttc gtaagaaaaa tgcaacgatt gcacgcggat aatattctgc ggaatgcgca
    22801 gcacagagtg aagacgtgac ggattccgga acagaaaaac agtcgtagaa tcagcggcaa
    22861 gccggaaagg ttccggtgag gcgcaatgtt gcgggggctt tatccctggt ggcattggtt
    22921 gctggagaga gaaaaccccc gcacgttgca ggtatgcacc tgacaacatc acgggggcta
    22981 atcttgaacc caaacaactc aagaatagcc gcgaaacgtt ggcattacaa cacaggcggc
    23041 tatatgacgc tcgcagaact gggcatggcc ttctggcatg atttagcggc tccggtcatt
    23101 gctggcattc ttgccagtat gatcgtgaac tggctgaata agcggaagta acgtgtcatg
    23161 cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc aggccgcaga ggttaaactc
    23221 tgcggccttt ttcgtgctta ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg
    23281 tacggtttat cttggtattg aataactact gcatttgagt tctcggagac cggtgctgtt
    23341 tgtggcaacc cactggtgag ttttttccag tcaacattgt cttcggtgaa aatcttgcca
    23401 tcaagaacgc gaaccaccag atcggagata gccaggaagc tgctcggttg gtcaatgaca
    23461 atcggtgccc cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac ggggacgtct
    23521 gtgatagacg ggctagggat atcacgtagg ccagatacct gcattctgtc gcccttcagc
    23581 gcgccgccgt gttccggcac cacgaccacc atcactttac gacccgattt ctccagttca
    23641 gtaaagaagg cgtccagttc atcaaagaat ttctgcgccc gcgctttgta atccgctgtt
    23701 ttgctgaccc ccggataatg gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca
    23761 ctacggctgt ttttatcttt ttcggtaacg tccagccagc ggttaagcac ggcggtatcg
    23821 tcataaaccg gcgaaccatc aaagcccagc aaaataaccg gcagatttgt ttgatccatc
    23881 aattcagtct gcatgccgcc attttcgcga acttctttca aaaaaccgcc gaactggccg
    23941 ttatgtccca tcatcaggtg ctgggtaaag cccagtttcg aaaggttatc aaacagatag
    24001 cagtcgttat ttgccggttg atacagatta gtgtgcgaag tctgcccgca gctggcgcgc
    24061 agtaaacgga tcgccgccgg gccactgtag gaggtggcgg agttaaagtt cttgaaatca
    24121 atatcgaaat gcgaccacag tggatgcgac atcaacccgg cggcttctat atccgaccag
    24181 gaaagcgaac agatgttaat caccagtagt tcaaatggct gagcatcagc gggcagcgaa
    24241 gacgggaagg tcgatttacg tttcgcctcc gcgttataga aattattcag ccaggcgtta
    24301 aggttcgccg ttgttggcgg tgcagtttgt gcgggcatat cacccactac cggtgcgcca
    24361 cccgtcgccg caacggttgc cgctgcgtta ccacccgtcg ttgttacagt ggtcgtcggt
    24421 tgtccggctg gccacaagga gaaacttggt cccgccaagg taagtacgtt cagccatagc
    24481 agtatggcaa ccacaaaaac ggtaatgcga atccattgtg acaggaataa ccaggccact
    24541 aataaaacaa aaatggcccc aatcatctgc cagttaataa agcgtgtgac aaggtcgatt
    24601 aaataatcgg tactgaaccc cgccacctgc gaaccctggc tcattatgct ttccgggcca
    24661 ggcaaccaga tgtcatgcca gaacaaagca aagccgatcg gcagggcaat ccagtggcgc
    24721 aagcgatgca ggctgtaacg cggaatgggc atcagcagaa acgcggcaaa caccaaattg
    24781 aggagcgggt ggaagttaag atatcccgcc cacaacaggc caaacttaac cagaaaatag
    24841 aagttccagc cggaaaggcc gcgccagtat tgccagaggg aagaaggcat ggcggtattt
    24901 tgcgtaaatt gagtcatttt ttggttgccc tggctttttc cgtgcggcgt aacgtcccgg
    24961 ccggtttaac ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca
    25021 aagagtgccg cgccagatag cccagcggga aaaatatcag tgcgcaaacg ataataattt
    25081 caatgatatc gctgatggtc atcatgatga gcgctccaca gcatcatcta acagtcgcat
    25141 gggttctggt attcgtcgcc agtggcgacc atcgtgctcg gcattgatga ccggtttaga
    25201 actttgcgtt aaaggcagcg gcattcccca ttgttctggg gcaagcaggc gcatctgcac
    25261 cagctcggca ctgatttgat catcttcaaa ccagaccata cggtttgaga aaatgtcgcc
    25321 agtaggcaat gggaaaatat gattcaacgc ggtatccaga tcgttaatcc gacagaatga
    25381 gagaaacagc accagccgat taccgccaat ggtcatgata tcgccggtac ggttagggcg
    25441 acatagcgtc agggcttgct caacgcggat acccggtacc ggacgtaggg caaccagaac
    25501 gcctttaccg tgggcaggca agagagggtt attcatcatg ttgttgacgg cattacagaa
    25561 cacatcccac ttctggaaac cacgcagttt gagcggctgg gtcattgaca gcaaggtagt
    25621 gatatcttcc ggtacatagc gactaaactt ctgcccttgc acgctttcga tcatcgtcag
    25681 acaacgggag agtggcgcat tccacggaat aaccatattt gcaccgcagg ccaataacaa
    25741 acgttcatcg gtggcgcgca ggctagcggt attttcccgc acgaggattt tcatcgcact
    25801 accgcgctgg cgacgcaggg tatgaatgct gcgggccagt ggctcgattt gcgcattttg
    25861 ctgtaaagaa aagaccaccg tcgccgcctg agcggtacgg gcttcattga acaggacttc
    25921 gttattgttg aacagttgcc agtgttccga tagcggcggc gcaccttcca gtacagcaac
    25981 attactcaga atgcgttttt cgtcgctgcg tggttggatc tccgcctctt cgctttgaac
    26041 taatgtccag ataccatttt gttgctgaac gctaagctgc tgacgggcgc tgaccccttt
    26101 ttcgttgcac cagaaggcaa tatccagcaa atgttggtca ccctgaaaac gcaaactggc
    26161 aagaccaaaa agtgaacggt attcctcaag caataatgaa aattgtttat cgttattatt
    26221 tccgggatta attaccaaaa gcgaacaatg gtttaacctg ctccatttat tcattttatc
    26281 caaccatgag cgaagccgct cggcaggaat attttgccat gcgttatttg cgcaaacaag
    26341 aataaaaagg taattatggg gatcaataga acactgcaaa tcacgggcca aatagtatag
    26401 acctttttca tgatttagca ttgaaaataa ttttattttt tccggccctt gagaatcatc
    26461 taattgaaag attttcgccg gatcgctgtc catgctaatg accgcgacgt gtgcggtttc
    26521 agcctgggat gcaattgttt gattcgccag actgatagca tcttcatggc gatcgacgtt
    26581 aaaccaccag acgccgcctg ctggcatatg tcgcagctca tcccataatg atgagatacc
    26641 gatagagaat acagggtcca caatgtccct catgtcacca ttttgtctga ttatcagttt
    26701 actagcgaaa gctaagaaat aaacctacca ttgaaattaa aacttatcag atttagcatg
    26761 tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt taactttttc
    26821 tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa acaataaacc
    26881 ggcgaaatgc tcacggttag aattgtctca tgaatggtac ggttatttca tagggatcaa
    26941 gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc tatatcttcc
    27001 agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat tatgccgata
    27061 tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg gcagagtttg
    27121 cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg tgcggggagg
    27181 cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa
    27241 tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta atgttgattt
    27301 tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc gtgacgctgg
    27361 gttgtgctac acctcgcagc tcgatttgct gccttttggt cagttatcca ttgaagaaca
    27421 agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct tacagcaact
    27481 aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg cctcgcagat
    27541 aacccaccag ctgctgagtt tgtgcgagca ctcgctggca atcgtcaatg tggatgccaa
    27601 ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt tgattaatga
    27661 cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc aaagccagcg
    27721 ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa
    27781 gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac tgacgctggc
    27841 gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg catcatgagt
    27901 atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc
    27961 gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt ctggatgatc
    28021 ctggcctgga tttttattcc gctggagcac ccgcgctggc agcgtattcg cgcagaacat
    28081 aaaaacctgt atacgcatat caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat
    28141 ctcattcaaa catgctggtt attgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc
    28201 agcgggcatt ttcaggtctg caaaatattc gtggacgtta ccatcaatgg atgaacgagc
    28261 tgcctgagcg cgttagccat aaaacacagc atctggatga aaaaaaagag ctcggtcatt
    28321 tgagtgccgg ggcgcggcgg ttgatcctcg gtatcatcgt caccttctcg ctgattctgg
    28381 cgttaatctg cgttactcag ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc
    28441 tgtggggggt agcgctgatc gtacggcgga tgccggggcg cttctcggcg ctaatgttga
    28501 ttgtgctgtc gctgaccgtt tcttgccgtt atatctggtg gcgttacacc tctacgctga
    28561 actgggacga tccggtcagc ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg
    28621 cgtggattgt gctggtgctc ggctacttcc aggtagtatg gccgctgaat cgtcagccgg
    28681 tgccattgcc gaaggatatg tcgctgtggc cgtcagtgga tatctttgtc ccgacttaca
    28741 acgaagatct caatgtggtg aaaaatacca tttacgcctc gctgggtatc gactggccga
    28801 aagacaaact gaacatctgg atcctcgatg acggcggcat ggaagagttt cgccagtttg
    28861 cgcaaaacgt gggggtgaaa tatatcgccc gcaccactca tgaacatgcg aaagcgggca
    28921 acatcaacaa tgcgctgaaa tatgccaaag gcgagttcgt gtcgattttc gactgcgacc
    28981 acgtaccaac gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc
    29041 tggcgatgat gcagactcca caccacttct tctcgccgga cccgtttgaa cgcaacctgg
    29101 gacgttttcg taaaacgccg aacgaaggca cgctgttcta tggtctggtg caggatggca
    29161 acgatatgtg ggacgccact ttcttctgcg gttcctgtgc ggtgattcgt cgtaagccgc
    29221 tggatgaaat tggcggcatt gctgtcgaaa ccgtgactga agatgcgcat acttctctgc
    29281 gtttgcaccg tcgtggctat acctccgcgt atatgcgtat tccgcaggcg gcggggctgg
    29341 cgaccgaaag tctgtcggcg catatcggtc agcgtattcg ctgggcgcgc gggatggtat
    29401 aaatcttccg tctcgataac ccgctcaccg gtaaaggact gaagtttgct cagcggctgt
    29461 gctacgtcaa cgccatgttc cacttcttgt cgggcattcc acggctgatc ttcctgactg
    29521 cgccgctggc gttcctgctg cttcatgcct acatcatcta tgcgccagcg ttgatgatcg
    29581 ccctattcgt gctgccgcat atgatccatg ccagcctgac caactcgaag atccagggca
    29641 aatatcgcca ctctttctgg agtgaaatct acgaaacggt gctggcgtgg tatatcgcac
    29701 caccgacgct ggtggcgctg attaacccgc acaaaggcaa atttaacgtc accgccaaag
    29761 gtggactggt ggaagaagag tacgtcgact gggtgatctc gcggccctac atcttccttg
    29821 ttctactcaa cctggtgggc gttgcggtag gcatctggcg ctacttctat ggcccgccaa
    29881 ccgagatgct caccgtggtc gtcagtatgg tgtgggtgtt ctacaacctg attgttcttg
    29941 gcggcgcagt tgcggtatcg gtagaaagca aacaggtacg ccgatcgaac cgcgtggaga
    30001 tgacgatgcc cgcggcaatt gcccgcgaag atggtcacct cttctcgtgt accgttcagg
    30061 atttctccga cggtggtttg gggatcaaga tcaacggtca ggcgcagatt ctggaagggc
    30121 agaaagtgaa tctgttgctt aaacgcggtc agcaggaata cgtcttcccg acccaggtgg
    30181 cgcgcgtgat gggtaatgaa gttgggctga aattaatgcc gctcaccacc cagcaacata
    30241 tcgattttgt gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg caggacagct
    30301 acccggaaga taagccgctg gaaagtctgc tggatattct gaagctcggc ttccgtggct
    30361 accgccatct ggcggagttt gcgccttctt cggtgaaggg catattccgt gtgctgactt
    30421 ctctggtttc ctgggttgta tcgtttattc cgcgccgccc ggagcggagc gaaacggcac
    30481 aaccatcgga tcaggctttg gctcaacaat gatgataacg cgatgaaaag aaaactattc
    30541 tggatttgtg cagtggctat ggggatgagt gcgttcccct ctttcatgac gcaggcgacg
    30601 ccagcaacgc aaccactgat caatgctgag ccagctgtag ccgcccagac ggaacaaaat
    30661 ccgcaggtgg ggcaagtgat gtcaggcgag cagggagctg atgcccccat cgtggcgcag
    30721 aacggtcctt cgcgtgatgt gaagctgacc tttgcgcaaa tcgcgccgcc gccgggcagc
    30781 atggtgctac gtggtattaa cccgaacggc agcattgagt ttggtatgcg cagcgatgaa
    30841 gtggtgacga aggcgatgct caacttcgaa tacacgccat cgccatcgtt actgcctgtc
    30901 cagtcgcagt taaaggttta tctcaatgat gagctgatgg gcgtgctgcc agtgaccaaa
    30961 gaacagttgg gtaaaaaaac gctggcgcaa atgcccatta atccactgtt tattaccgac
    31021 ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga aaacccggcc
    31081 agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac ctatcagacc
    31141 ctgaatgtga agaatgacct gtcacacttc ccggtgccat tctttgaccc gcgcgataac
    31201 cgtactaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgggct gcaacaagca
    31261 tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca gaacttcccg
    31321 gtgctctata accaattgcc ggatcgcaat gccattgtct ttgcaaccaa cgacaaacgg
    31381 ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat gattaaccat
    31441 ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa agacctgttg
    31501 caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag cgtggtagtg
    31561 aatgaagtga aaccgctgct accgcgtaag ccgtacgatg cgccgaactg ggtacgtacc
    31621 gatcgtccgg tcacatttgg cgaactgaaa acctatgaag aacagttaca atccagcggc
    31681 cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta cctgatgcgc
    31741 agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt gaaagacagt
    31801 tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct gagcagcaaa
    31861 caggaggcga accgtctgct gctgcgtatt ccggtattac aaggtttgct ggatggcaaa
    31921 acagatgtct ctattccggc gctgaaactg ggcgcgacca accagctgcg cttcgacttt
    31981 gagtatatga acccgatgcc aggcggttcg gtagataact gtattacctt ccagccggtg
    32041 cagaatcatg tggtgattgg tgacgattcc accatcgact tctcgaagta ttaccacttc
    32101 atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccattcag ccggatggcg
    32161 gatctgtcgc aaaccatcac cgtgatgccg aaagcgccta acgaagcaca gatggaaacg
    32221 ttgctgaata ctgttggttt tattggcgca cagacgggct tcccggcgat taatctgacg
    32281 gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat catcggtggt
    32341 atcccggaca aactgaaaga cgataagcag atcgacctat tggtgcaggc gaccgaaagc
    32401 tgggtgaaaa caccgatgcg ccagaccccg ttccccggca ttgtgccgga cgagagcgat
    32461 cgcgcggcag aaacccagtc aactctgacc tcttccggtg cgatggcggc ggtgattggc
    32521 ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgttggcaga tagcccacgc
    32581 ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac catgttcggt
    32641 tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga cgtttattac
    32701 gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca tccgattctg
    32761 ctggcggtgc tggcgactat cagtgtgata ttgctggcat gggtactgtg gcgtctgctg
    32821 cgaattatta gtagtcgtcg tcttaacccg gataacgagt aattgaagat gaatgtgttg
    32881 cgtagtggaa tcgtgacgat gctgttgttg gctgccttta gtgttcaggc agcctgtacc
    32941 tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg cgtcatcgac
    33001 cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggtat gttctttgcc
    33061 ctggcggcta acgaccgtgc agctttcgat aatattctcg actggacgca gaacaatctc
    33121 gctcagggtt ctttaaaaga acgtttgccc gcctggctgt ggggcaagaa agagaacagt
    33181 aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat ggcctggtcg
    33241 ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg cagcgcgttg
    33301 ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggttc catgttgtta
    33361 ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag ctacatgccg
    33421 ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct gcgcgaaacc
    33481 aatcaacatt tattgctgga aaccgccccg aaaggctttt cgccagactg ggtgcgctat
    33541 gagaaagaca aaggctggca gctaaaagcc gaaaaaacat tgatcagcag ctacgacgct
    33601 atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa agcgcggatg
    33661 ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc gccggaaaaa
    33721 gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg tcggtttttc tgccgccatg
    33781 ctgccttttt tacaaaaccg cgatgcgcag gcagttcagc gccagcgcgt ggccgataac
    33841 tttcccggca gcgataccta ttacaactat gtgctgaccc tgtttggaca aggctgggat
    33901 caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg ccaggaatgc
    33961 gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg
    34021 aggcagcacc aaccatcagc agaccctgcg gatggacagc ggcaacacta atgccgtgcg
    34081 cgggctggcg aatatttacc gccagcagtc gccagaaaaa gctgaagcgt ttatcgcctc
    34141 gctctctgcc agccagcggc gcagcattga tgatatcgaa cgcagcctgc aaaacgaccg
    34201 tctggcacag caggcagagg tactggaaaa ccagggcaaa tgggcgcagg cggcagcact
    34261 tcagcggcaa cgactggcgc tgggccccgg cagcgtatgg attacttacc gactttcgca
    34321 ggatctctgg caggccggac aacgcagcca ggccgatacg ttaatgcgca atctggcgca
    34381 gcagaagccg aacaacccgg agcaggttta cgcttacggg ctgtacctct caggtcacaa
    34441 ccaggacaga gcggcgctgg cgcatatcaa cagcctgccg cgcgcgcagt ggaacagcaa
    34501 tattcaggag ctggttaatc gactgcaaag cgatcaggtg ctggaaaccg ctaatcgcct
    34561 gcgagaaagc ggcaaagagg ctgaagcgga agcgatgctg cgtcagcaac caccttccac
    34621 gcgtattgac ctcacgctgg ctgactgggc gtaacaacga cgtgattaca ccgccgcccg
    34681 cactgcatat cagaatgtcc tgacgcggga gccaactaac gccgatgcca ttcttggtct
    34741 gacggaagtg gatattgctg ccggtgacaa agcggcggca cgtagccagc tggcgaaact
    34801 gcctgccacc gataacacct cgctgaacac tcagcggcgc gtggcgctgg cacaggcgca
    34861 gcttggagat accgcagcgg cgcagcagac gtttaataag ttgatcccgc aggcaaaatc
    34921 tcagccaccg tcgatggaaa gcgcgatggt actgcgcgac ggtgcgaagt ttgaagcgca
    34981 ggcgggcgat ccaaagcagg cgctggaaac ctacaaagac gccatggtcg catccggtgt
    35041 gactacgacg cgtccgcagg ataacgacac ctttacccga ctgacccgta acgacgagaa
    35101 agatgactgg ctgaaacgcg gcgtgcgcag cgatgcggcg gacctctatc gccagcagga
    35161 tcttaacgtc acccttgagc acgattactg gggttcgagc ggcaccggtg gttactccga
    35221 tctgaaagcg cacactacca tgttgcaggt ggatgcgccg tattctgacg ggcggatgtt
    35281 ctttcgcagt gatttcgtca atatgaacgt cggcagtttc tccactaatg ccgatggcaa
    35341 atgggatgac aactggggca cctgtacatt acaggactgt agcggcaacc gcagccagtc
    35401 ggattccggt gccagcgtgg cggtcggctg gcgaaatgac gtctggagct gggatatcgg
    35461 taccacgccg atgggcttca acgtggtgga tgtggtcggc ggcatcagtt acagcgatga
    35521 tatcgggccg ctgggttaca ccgttaacgc ccaccgtcgg cccatctcca gttctttgct
    35581 ggcctttggt gggcaaaaag actccccgag caataccggg aaaaaatggg gtggcgtacg
    35641 tgccgacggt gtggggctaa gtctgagcta cgataaaggt gaagtaaacg gcgtctgggc
    35701 atcgcttagt ggcgaccagt taaccggtaa aaatgtcgaa gataactggc gcgtgcgctg
    35761 gatgacgggc tattactata aggtcattaa ccagaacaat cgccgcgtca caatcggcct
    35821 gaacaacatg atctggcatt acgacaaaga tctgagtggc tactcactcg gtcagggcgg
    35881 ttactacagt ccgcaggaat acctgtcgtt tgccataccg gtgatgtggc gggagcgcac
    35941 ggaaaactgg tcgtgggagc tgggggcgtc tggctcgtgg tcgcattcac gcaccaaaac
    36001 catgccgcgt tatccgctga tgaatctgat cccgaccgac tggcaggaag aagctgcgcg
    36061 gcaatccaac gatggcggca gcagtcaggg cttcggctac acggcgcggg cattacttga
    36121 acgacgtgtt acttccaact ggtttgttgg tacggcaatt gatatccagc aggcgaaaga
    36181 ttacgcaccc agccatttcc tgctctacgt acgttattcc gccgccggat ggcagggtga
    36241 catggattta ccgccgcagc cgctgatacc ttacgccgac tggtaagttt tcagatagcg
    36301 cctctcttaa tgccgctgcg atcgggtata ctcgggcggc aatctgggat ttccgggggg
    36361 agacaatttg cgcgtaagtc gctcgttaac aatcaagcag atggcaatgg tggcagccgt
    36421 tgtcctggtg ttcgttttta ttttttgcac cgttttgctg ttccatctgg tccagcagaa
    36481 tcgctataac acggctacgc aactggaaag cattgctcgc tctgtccgcg aacccttatc
    36541 ttcagctatt ttgaaaggcg atattcccga agcggaagct attcttgcca gcattaaacc
    36601 ggcaggcgtg gtcagccgtg ccgatgtagt gctgcctaac cagttccagg cgctgcgtaa
    36661 aagttttatt ccagagcgtc cggtgccggt aatggttact cgcctgtttg agctaccggt
    36721 tcaaatctcg ctgggcgtct actcgctcga acgtccggca aatccgcagc caatagccta
    36781 tctggtgcta caggcggatt ccttccgtat gtataagttc gtgatgagca ctctctcaac
    36841 gttagtgacc atttacttac ttttgtcgct tatcctgacc gtcgccatca gctggtgcat
    36901 taaccgcctg attttgcatc cgttacgcaa tattgctcgc gaacttaacg ccatcccagc
    36961 ccaggagctt gttggtcacc aactggcatt accgcgtctg catcaggacg atgaaatcgg
    37021 tatgttggtg cgcagttaca acctcaacca gcaattgctg cagcgccatt atgaagaaca
    37081 gaacgaaaat gcgatgcgct tcccggtgtc ggatttgccg aacaaagcct tgctgatgga
    37141 gatgctggag caggttgtcg cgcgtaaaca aaccaccgcg ctgatgatca tcacctgtga
    37201 aaccctgcgt gatactgcgg gcgtgctgaa agaggcgcaa cgagaaattc tgctgctgac
    37261 gctggtggaa aaactcaaat cggtactgtc gccacgtatg atcctcgcgc agattagcgg
    37321 ttatgacttt gctgtcattg ccaacggtgt acaggaaccg tggcacgcaa tcactttggg
    37381 tcagcaagta ctcactatca tgagcgagcg cctgccgatt gaacgtattc aactccgtcc
    37441 gcactgtagc attggcgtgg cgatgttcta cggcgatctc accgccgaac agctttacag
    37501 tcgcgctatt tctgcggcat ttaccgctcg ccataaaggc aagaatcaga ttcagttctt
    37561 tgatccgcag cagatggaag cagcccagaa gcggttgacg gaagagagcg atatccttaa
    37621 tgcactggaa aatcatcagt ttgcaatttg gttacagcca caggtcgaga tgaccagcgg
    37681 taaactggtc agtgcggaag tgttactgcg tatccagcaa ccggatggca gttgggacct
    37741 gccggatggc ttaatcgatc gcattgagtg ctgtgggctg atggttaccg tcggtcactg
    37801 ggtgctggaa gagtcctgtc gattgcttgc agcctggcaa gagcgcggca ttatgctgcc
    37861 cttgtcggta aacttctctg cgctgcaact gatgcacccg aatatggtgg cggatatgct
    37921 ggaactgtta acccgctatc gcattcagcc gggaacactg attctggaag tgacagaaag
    37981 ccgacgtatt gacgaccctc atgctgcggt ggcaatcctc cgtccgctgc gcaatgccgg
    38041 agttcgggtg gcgctggatg atttcggcat gggctacgca gggctgcgtc agctgcagca
    38101 tatgaaatcg ttgccaatcg acgtactgaa aatcgacaaa atgtttgttg aaggcttgcc
    38161 ggaagatagc agcatgattg ctgcaattat catgctggcg cagagcctga acttacaaat
    38221 gattgccgaa ggcgtggaga ctgaagcaca acgagactgg ctggcaaaag cgggcgttgg
    38281 tattgcccag ggcttccttt ttgctcgccc actccctatt gaaatcttcg aagagagtta
    38341 cctggaagaa aagtagctac cccaaactga ttacaaaact ttaaaaagtg ctggtttgtg
    38401 cgagccagct caaacttttt aacctttttg tttcaattat gatccaggta catttctgtg
    38461 atgttgtctg ggtgttattt taaggccgca ggtacccccc cataacctta caagacctgt
    38521 ggttttacta aaggacaccc tatgaaaacc tctctgttta aaagccttta ctttcaggtc
    38581 ctgacagcga tagccattgg tattctcctt ggccatttct atcctgaaat aggcgagcaa
    38641 atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc
    38701 ttttgtaccg tcgtaacggg cattgcgggc atggaaagca tgaaggcggt cggtcgtacc
    38761 ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca tcgcgctgat tattggtctt
    38821 atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat
    38881 gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc agggcattgt cgccttcatt
    38941 atggatgtca tcccggcgag cgtcattggc gcatttgcca gcggtaacat tctgcaggtg
    39001 ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc tgggcagcaa aggccaactg
    39061 atttttaacg tgatcgaaag tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg
    39121 cgtctggcac ctattggtgc gttcggggca atggcgttta ccatcggtaa atacggcgtc
    39181 ggcacactgg tgcaactggg gcagctgatt atctgtttct acattacctg tatcctgttt
    39241 gtggtgctgg tattgggttc aatcgctaaa gcgactggtt tcagtatctt caaatttatc
    39301 cgctacatcc gtgaagaact gctgattgta ctggggactt catcttccga gtcggcgctg
    39361 ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta aatcggtggt ggggctggtt
    39421 atcccgacag gctactcgtt taaccttgat ggcacatcga tatacctgac aatggcggcg
    39481 gtgtttatcg cccaggccac taacagtcag atggatatcg tccaccaaat cacgctgtta
    39541 atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg
    39601 ctggcggcga cgctctctgc ggtgggccat ttgccggtag cgggtctggc gctgatcctc
    39661 ggtatcgacc gctttatgtc agaagctcgt gcgctaacta acctggtcgg taacggcgta
    39721 gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg
    39781 ctgaataatc gtgcgccgga tggcaaaacg cacgaattat cctcttaatc tcaccactta
    39841 tgcccgcact cccttccccg agtgcgggtg tttgcgcata attgacccct gtgcctgaac
    39901 ttaccacctg ggtgagttca ggtttatttc acttcttccg tgacattttc atgttcttgc
    39961 ggtctaacac gaagtgtttt taacgtcatt tagactgacc gacaacgggc agttttttta
    40021 ttaccaggat agttgatcag gggttcacat gcagggcata aaaattcgac ttttagcggg
    40081 cggtttgctg atgatggcca ctgctggcta tgtgcaggca gatgcgctcc agcctgatcc
    40141 agcatggcaa caggggacgc tttccaacgg tttacagtgg caagtgctga ccacccccca
    40201 gcgtcccagc gatcgtgttg aaattcgcct gctggttaat accggttcgc tcgccgaaag
    40261 tacacaacag agcggttaca gtcacgccat ccctcgtatt gcgctaacgc aaagcggtgg
    40321 ccttgacgca gcacaggcgc gttcattgtg gcagcagggg atcgacccta aacgcccgat
    40381 gccgccggta attgtctctt atgacaccac gctgtttaat ctgagtttgc ccaataaccg
    40441 taacgatttg ctgaaagaag cgctctctta tctggcaaat gccactggca aattgaccat
    40501 cacaccagaa accatcaacc acgcgctgca aagtcaggac atggtggcaa cctggcctgc
    40561 cgatactaaa gagggctggt ggcgctatcg tctgaaaggg tcaaccttgt taggtcacga
    40621 tcctgccgat ccgctgaaac aacccgttga agcggaaaaa attaaagatt tctatcagaa
    40681 atggtacacc ccggatgcaa tgacgctact ggtggtggga aacgtggatg cgcgctcggt
    40741 tgtcgaccaa atcaacaaaa cgtttggcga actgaaaggc aaacgtgaaa cgccagctcc
    40801 ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt attatgactg acgcggtgcg
    40861 tcaggaccgg ttatctatca tgtgggatac gccgtggcag ccgattcgtg aatcagccgc
    40921 actgctgcgc tactggcgtg cggacctggc ccgcgaggcg ctgttctggc atgttcagca
    40981 agcgttaagc gccagtgaca gcaaagacat cggtcttgga tttgactgcc gtgtgctgta
    41041 tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac gacaagctga acagcaacct
    41101 taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg aagaagagtt
    41161 caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa ctgtttgccg cctatgcacg
    41221 ggctgatacc gatattctga tgggccagcg gatgcgttcg ttgcaaaatc aggtcgtcga
    41281 tatcgcgccg gagcagtatc agaaactgcg gcaggatttc cttaacagcc tgactgtgga
    41341 gatgttaaat caggatctgc gtcagcagtt gtcgaatgat atggcgttaa tactgctgca
    41401 gccgaaaggc gagccggaat ttaacatgaa agcgttgcag gcggcctggg atcaaatcat
    41461 ggccccatct accgccgctg cgaccacctc tgtcgccacg gatgacgtac atcctgaagt
    41521 gacggatatt ccacctgcac agtaatgcct gatggtgatg agttagcaca accatcaggt
    41581 atttatggat gagctggata gaggggtaac actttatcca gccttttgca tatgctgcgt
    41641 ttacgctggc atcgcctcac gcgggataat cgcgccgcga tactgaataa cggtacttgc
    41701 ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg cccgtcaaac gtaccgccag
    41761 ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg atcacttttt ctttcggcag
    41821 tttcaccgcc ggaacatcaa ctaacgcttc accagcaatg gacaccaggc aagaatccgc
    41881 cccgcgtttc accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa tgacgtcttc
    41941 caccggctgt tgaccccaca gcgcgtcttc gtcgtccagc gtcaggaagg cgatatccgt
    42001 gcattccagc atttgctggt acacctgctg tgtctcttct ttgctggccc acaggcgcgg
    42061 acgatagtta ttgtcgaaaa tcacttttcc gcctttggcg cggcattcgc gcagcaggga
    42121 aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc tcaggtagag
    42181 ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac tgctcgctcg ccagccagaa
    42241 tttggcggcg gcttcgttac gccagtagta gaacgtacgt tcgccggtgc tgtcggtttc
    42301 aatgtagtaa aggcccggaa gacggttttc catccgttgg gtcagggaag tatcaacgtt
    42361 ctcgccgtgc caggcgtcca gcatctgctg gctaaaactg tccgttccca gcgccgttac
    42421 gtaatgaacg gttaatgccg caggatcgac ctgacgggcg atatagacgg aagtgttcag
    42481 ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct caatcatgca
    42541 ttcgccaatc acggcaatct ttttggacat agtcgtgaac ctgatctgta aaaaattagc
    42601 gttagtttgc gctgtggtgg tttgctggtc aaccatatta aaacagtgtt ccattatttt
    42661 tttgagccag aacgtattcc tgaaagattc cgttgtggag tgaggaaatt taccccggcc
    42721 aggcggtaaa gttctgccct tacgcgccga taatctttgt cgagtccggg cagcatcact
    42781 tttaaacaca ggacatcttt gatgataagg caggttatcc agcgaataag caaccctgaa
    42841 gcaagcatcg agagcttgca ggaacggcgt ttttggttgc agtgtgagcg tgcttacacc
    42901 tggcagccga tctatcaaac atgcgggcgg ttaatggccg tggagctatt aacggtggtc
    42961 acgcatccct tgaacccttc gcaacgcctg ccgccggatc gctattttac tgaaatcacc
    43021 gtcagccatc ggatggaggt tgtgaaagag cagattgatt tgctggcgca aaaagccgac
    43081 ttctttgtag agcacggcct gctggcatcg gtcaatattg atggccctac gctcatcgcc
    43141 ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc ttccctggct gcgtttcgaa
    43201 ctggtggagc atatccgtct gccgaaagat tcaacctttg cctcgatgtg tgaatttggc
    43261 ccgctgtggc tggatgattt tggtaccggg atggcaaatt tctctgcact aagtgaagtg
    43321 cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca gtcgccggaa
    43381 ggccgcacac tcttttctca gcttttacat ctaatgaatc gctattgtcg cggggtgatt
    43441 gtcgagggcg tagaaacacc ggaagagtgg cgtgatgttc agaactcgcc cgcattcgcc
    43501 gcacaaggct ggtttctttc acgcccggca ccgatggaaa cgctgaatac ggcggttctg
    43561 gcgctataag ctgcctcatt ttccgccggg ctggactatc tttaggactg gcacaggaag
    43621 aatatgagga agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat ttcaggggct
    43681 ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg gaatcgactc
    43741 aaaccgacca tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc tatccgtggc
    43801 aatctgggcg tggtgtggga gcggcaaaaa caagaaactg gctggcgcag ctgggtgccg
    43861 tggcctcatg tacatgcaga agacatcatt cttggtaatc caccggatat tcccgaagtc
    43921 acgatggtgc atttgccgcg agtagaggca acgctggcac cgctggcgct gctgaccaaa
    43981 acggtctggc tgccgtggat caagctcgaa aagcccgacg cgcgcctgat tcgcctctct
    44041 gaaaagaaca ataactggac gtttaatctt gccaacgatg acaacaaaga cgcgaatgca
    44101 aagccgtcgg catggtcgtt tcggctggat aatattcttt tcgatcaagg gcggatcgcc
    44161 attgatgaca aagtaagcaa agcggatctg gaaatttttg ttgatccgtt aggcaagccg
    44221 ctgccattca gcgaagttac tggatcgaaa ggtaaagcgg ataaagaaaa ggtgggcgat
    44281 tacatttttg gcctgaaggc gcagggacgt tataacggtg aaccgctcac gggtacggga
    44341 aaaataggcg gtatgctggc gctgcgtggc gaagggacgc cgtttccggt acaggctgat
    44401 ttccgttcag gtaatacccg tgttgctttt gatggcgtcg tgaatgaccc aatgaagatg
    44461 ggcggtgtcg atttacggct taaattttct ggcgattcac tgggtgatct ctatgaactg
    44521 acgggcgttc tgctgcccga taccccgccg tttgaaacgg atggtcggct ggtagcgaaa
    44581 atcgacactg aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg aattggcgat
    44641 agcgatatcc acggttctct ggtctacacc accggaaagc cacgaccaaa actggaaggt
    44701 gatgtcgagt cgcggcaatt gcggctggcg gacctgggac cgttgattgg cgttgattcc
    44761 gggaaagggg cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa aagcgttcag
    44821 cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga cgttatggat
    44881 gccgatgttc gcttcaaagg gcggcgcatt gagcatggca gtagcctgcc gattagcgat
    44941 ctttctactc atatcatcct caaaaatgct gacctgcgcc tgcaaccgct gaaatttggc
    45001 atggcgggcg gcagcattgc ggcgaatatt catctggaag gcgataaaaa gccgatgcag
    45061 gggcgggcag atattcaggc tcgtcgactg aaactgaaag aactgatgcc cgatgtggaa
    45121 ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg aactacgcgg tagcggtaac
    45181 tcggtggcgg cgcttttagg caacagtaac ggcaacctga aactgttgat gaatgacggg
    45241 ctggtgagcc gcaacctgat ggagattgtt gggctgaatg tcggcaacta cattgtcggt
    45301 gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg cggcgaatct gaatattgcc
    45361 aacggcgtgg cgcgcccgca gatttttgct ttcgatactg agaacgcgtt gattaatgtt
    45421 accggcacgg caagttttgc ttcggaacag ctggatttga ctattgatcc ggagagtaaa
    45481 gggattcgga ttatcacact gcgttcgccg ctgtatgtgc gtgggacgtt taaaaatccg
    45541 caggctgggg tgaaagccgg accgctgatt gcccgtggtg ctgttgctgc ggcactggca
    45601 acgctggtaa caccggcggc ggcgttactg gcactgatct caccttccga aggggaggct
    45661 aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat gataattgcc ggatgtctga
    45721 catccggcaa ttaaacagat ttagattttt ggcaaagtgt gctttgttca tgccggatgc
    45781 ggcgtgaacg ccttatccgg cctacaaatc cttgtgaatt caatatattg tatggaaatt
    45841 cagtcctgat aagcgtagcg catcaggctt ttcacactta ttacaacgac tgatgtcgcg
    45901 tctcatgggt cagcagcagg gcgattaacg tcaagccagc catcgccgcc agatataacc
    45961 ccaccgcacc taacccgtag ttagcctgca accaggctgc gatatacggc gcaacggaag
    46021 ccccgagaat cgacgatacg ttgtaagaga acgatgctcc ggtgtaacgc acttctgtcg
    46081 gaaacagctc tggtaacagc gcacccatcg gcccgaaggt cagccccatc agacttaacc
    46141 ccagcagcag gaaggcaaaa accagaatcg ggttgccaga accgagcagc gggttaaagg
    46201 cgaacagcgc gaacaggatg atcagcgtgg tgatgatcac catgctttta cggcgaccaa
    46261 aggcatcagc cagtaatccg gcgactggca ccatcacgcc aaaaccaata actgccatca
    46321 tcagcatcca caacacttcg ttacgcggca ggccaagccc aactggcgcg gcggcggtac
    46381 tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt ataggttgcc agcataatga
    46441 acgtacccag tacggttacg cgaacatgtt tggtcagcag cgtacccagc gggatcttca
    46501 cctgtttttt cgctttagcg actttctcaa acaccggcga ctcatgcagc gacacgcgaa
    46561 catacaggcc gataatgacc agcaccgccg agaagataaa tggcacacgc cagccccagc
    46621 tcataaactg ctcatcggtc agtagccagg aaagcagcaa gaaagtgccg ttggcaaaga
    46681 agaagccaat cggtgcgcct agctgtggga aggagccata cagtgcacgt ttgcgcggtg
    46741 gggcgttttc agtcgccagc agcgccgcac cgccccattc accgcctaag cccagaccct
    46801 gaccaaatcg agccagcgcc agcagcagtg gggcgaaaat accaatcgtg gcatagcccg
    46861 gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag caacgaggcg accagcgtcg
    46921 ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc agagccaatg gggcgcgcga
    46981 cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc tgccgttgga tcgccctgcg
    47041 ggaagaagat atgcggaaac acaataacgg ccgcagtggc gtaaatgtaa aagtcgaaga
    47101 actcaatggc cgtgccaatg agagaggcga caaggacttt attacgcgag ttgatcggcg
    47161 tgtattcttg ttcgtggtcg agtgttgtgg ctgttgcttg cataatcttt tcttattttt
    47221 tggctaacga atagccatat tacgcacagc aaaagtgata tttcaatgtg taacaaatcg
    47281 cggaaatggt caaaaaccag gcaaaaaggg gataatgttt aggccagtga tttttcttct
    47341 atgaaatgct tcacataatt ttataaatag tggataaaac tggtttcagg ttaaataaaa
    47401 gttaccaact ggatttatat gctggggtta tgagttggag tgaaattgtt tgctcccaaa
    47461 tagatggctg aagaggctct ggggaagagt ttcaagaggt tatgtgcgca ttatagccgg
    47521 atgcgatgct ggcgcatctt atccggccta cggggagcca ggccgaataa aatcgtatgc
    47581 cggataaggc gtttacgccg catccggcct attttaaggc tgcgttttcc ctggcattcg
    47641 cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg caaaacagcg tcaaacgggc
    47701 gaagaagtag aaaaatgcca tcagccccag cacggaacca aatgctgcgc cagaggggga
    47761 tttcatcagc gatggcaggg tgtaggtcat cacgatttta atcacttcaa aaccaatagc
    47821 agccagaaat gttccgcgaa tcagggcttt tttgcgcgga cggtggcgcg gcaaccgcca
    47881 gaagatccag aagaaaagca gatagttggc gaagatggaa atcgccaggc caatcaatcg
    47941 ccacgtcggt ttcagccact caatgctgtt caggtgcagg gcgctaataa tcatttgctg
    48001 cgccgaaccg gcaaccgagg tgatcgaaag cgtcacaatc agcgcaatca acaaaccaat
    48061 cagtgaaata aaatcacgca gatattttac ccagaacttc tcctgatctt gcggcgagcg
    48121 ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc aggttaccca tccagttgat
    48181 gccggaataa agtgccaccg ccaggccgac aagccctaca gtcgtacgct gctgaacggc
    48241 ggtgttgatg gtgtttttca acgtggcggc tagtgtcgga tcgctgatgt tttgcagaat
    48301 tttgtcgaag atatcctgta gcaacatcgg atgggaggcc agcacaaagc cccctgcggc
    48361 aaacgacacc atcaaaatcg ggatcatcga caaaaatgag aaataggtga tagccgcacc
    48421 aaactggtta cccagccgat cgttaaagcg ttctgtcgcg cgaataaggt gcgcaataac
    48481 cggttggcgt tggacttttt cggcagtggt ggtgacggtt tccagcgcct ggctaacttt
    48541 gccgcctttc tcgctattat ccaactgctt aatcggctcg tgctccagat cctgggtggg
    48601 acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc cttctgtcgt ggtcattctt
    48661 aaaaagtata gtcggtcagt ccaggtgctc tttcattacg ccagccagcc actgcataaa
    48721 caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa accagcgaaa gggagagcgg
    48781 ctcggcacgg tagcctggca acacttcaat aagccgcccg gcacgcaggg cttcgcgcac
    48841 ggcaatgcgc gggatctgaa taatccccag accggcaaga ccggcggcga gatagttttc
    48901 gctactgttt accgtcaaca tgccgccaga cttaaaccac tggacgccat taacgctggc
    48961 aacctcaaaa cctaacggat gtacacccag gtgcggtgta taacgcacta ttgcgtgtga
    49021 agtcagatca tcggggcttt gcggataacc aaagcgcgtc agatagtgcg gactggcaca
    49081 gttgaccatg gttagtttgc cgaggggacg ggcgataacg ccatcttccg gtaacgcgcc
    49141 agtgcgtatc acgcaatcaa aaccatcatg aagaatatct accggacggt tatggctact
    49201 cagttccagc tcaattcccg gatgctgata gagaaattcc gacagacgcg gcagtaacag
    49261 gtttttcgcg attcctgacg ggatgtcgat gcgtaattta ccgctgatac tggtggcatc
    49321 ctgttgaaac agaccgtcca gttcgctgag attactcaac acatcttttg cccgttgata
    49381 ataggtcatg ccttctggcg tgagtttgac ccgtcgcgta gtgcgctgga gaagctgggt
    49441 gccaagttga tgttccagtg cctgtatctg gcgcgaaaca cttccctttg gcaaagcaaa
    49501 gaaatccgcc gcgcgggaaa aactttccag ctccgcgact ttgatgaaca actgcattgc
    49561 gtgaatttta tccatttctt tgaccgattg ttgttcacag cgaaacagta acgcgcatat
    49621 cgcattgttt attgtttttt ctaacatcta atgccgggtt tcacaatgtt gtcatgacga
    49681 atgtggcgat ttctcagcag gaaaagggcg gaatagcttt atgagaaagg tgaatcccct
    49741 taaacagagc gtatttttca gtgattccct ttaaagtgaa tagaggacgt attggctatt
    49801 tgtgcttatt tttgtctcat tgccatttgt tattttggct tgtagtcgcg gaatttcatt
    49861 tttaattgca tgcgtactaa ccaccaatat aaggaaatcc tcaaaattgt tttaaatagt
    49921 gccctcgaca tgtaatttca ggcgtttagc tgtaactatt tatatcggta actattttct
    49981 acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa ttcttatttt ttctcgcggg
    50041 taataccctt ccactactta agtcttttta acgtattcag gtggagattc agttatgcaa
    50101 atagtcatgt ttgacaggca gtcaatattt attcatggaa taaaaatcag tttacagcag
    50161 cgtattccag gagtgagtat tcagggggtc agtcaggcag acgagttatg gcaaaagctg
    50221 gaaagttacc ctgaagcctt agttatgctc gatggcgatc aggatggtga gttttgctac
    50281 tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta aggtgttaat cacggcgacg
    50341 gattgcaata aacggtggtt acaggaagtt atccatttta atgtgctggc cattgtgcct
    50401 cgcgattcaa ccgtcgagac atttgcgttg gcggtaaata gtgcggcgat ggggatgatg
    50461 tttctgcctg gagactggcg gacgacgccg gaaaaggaca ttaaagatct caaatcgtta
    50521 agcgcccgcc agcgggagat tttaaccatg ttagcggcgg gcgaatcaaa taaggagatc
    50581 ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc atctggaatc cctgtatcgc
    50641 cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAEEDS010000022        49536 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEEDS010000022 DAEEDS010000000
VERSION     DAEEDS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49536
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.022"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KLH32_001345"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KLH32_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBE0138236.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVLLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /gene="fruB"
                     /locus_tag="KLH32_001346"
     CDS             1524..2654
                     /gene="fruB"
                     /locus_tag="KLH32_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBE0138237.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="KLH32_001347"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="KLH32_001347"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBE0138238.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="KLH32_001348"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="KLH32_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBE0138239.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="KLH32_001349"
     CDS             5724..6665
                     /locus_tag="KLH32_001349"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBE0138240.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6653..7591
                     /gene="psuG"
                     /locus_tag="KLH32_001350"
     CDS             6653..7591
                     /gene="psuG"
                     /locus_tag="KLH32_001350"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBE0138241.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="KLH32_001351"
     CDS             7685..8935
                     /locus_tag="KLH32_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE0138242.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KLH32_001352"
                     /pseudo
     CDS             complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KLH32_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="KLH32_001353"
                     /pseudo
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="KLH32_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10872..12122
                     /locus_tag="KLH32_001354"
     CDS             10872..12122
                     /locus_tag="KLH32_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE0138243.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="KLH32_001355"
     CDS             complement(12178..13266)
                     /locus_tag="KLH32_001355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBE0138244.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KLH32_001356"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KLH32_001356"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBE0138245.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="KLH32_001357"
     CDS             complement(14200..15249)
                     /locus_tag="KLH32_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBE0138246.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /locus_tag="KLH32_001358"
     CDS             15348..16229
                     /locus_tag="KLH32_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0138247.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="KLH32_001359"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="KLH32_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBE0138248.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="KLH32_001360"
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="KLH32_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBE0138249.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KLH32_001361"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KLH32_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="KLH32_001362"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="KLH32_001362"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBE0138250.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /locus_tag="KLH32_001363"
     CDS             21997..23073
                     /locus_tag="KLH32_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBE0138251.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="KLH32_001364"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="KLH32_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBE0138252.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="KLH32_001365"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="KLH32_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBE0138253.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="KLH32_001366"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="KLH32_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBE0138254.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="KLH32_001367"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="KLH32_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBE0138255.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KLH32_001368"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KLH32_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KLH32_001369"
     CDS             complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KLH32_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBE0138256.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="KLH32_001370"
     CDS             complement(30815..31054)
                     /locus_tag="KLH32_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBE0138257.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KLH32_001371"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KLH32_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBE0138258.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KLH32_001372"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KLH32_001372"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBE0138259.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="KLH32_001373"
     CDS             complement(32940..33635)
                     /locus_tag="KLH32_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBE0138260.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="KLH32_001374"
     CDS             complement(33632..34030)
                     /locus_tag="KLH32_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBE0138261.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="KLH32_001375"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="KLH32_001375"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBE0138262.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KLH32_001376"
     CDS             complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KLH32_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBE0138263.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35894..37330
                     /gene="mdtQ"
                     /locus_tag="KLH32_001377"
     CDS             35894..37330
                     /gene="mdtQ"
                     /locus_tag="KLH32_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBE0138264.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37383..38144
                     /locus_tag="KLH32_001378"
                     /pseudo
     CDS             37383..38144
                     /locus_tag="KLH32_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38261..38839)
                     /locus_tag="KLH32_001379"
     CDS             complement(38261..38839)
                     /locus_tag="KLH32_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0138265.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39009..39596
                     /locus_tag="KLH32_001380"
     CDS             39009..39596
                     /locus_tag="KLH32_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBE0138266.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39770..40702
                     /gene="pbpG"
                     /locus_tag="KLH32_001381"
     CDS             39770..40702
                     /gene="pbpG"
                     /locus_tag="KLH32_001381"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBE0138267.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KLH32_001382"
     CDS             complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KLH32_001382"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE0138268.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42651..44948
                     /gene="bglX"
                     /locus_tag="KLH32_001383"
     CDS             42651..44948
                     /gene="bglX"
                     /locus_tag="KLH32_001383"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBE0138269.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45200..46117
                     /gene="osmF"
                     /locus_tag="KLH32_001384"
     CDS             45200..46117
                     /gene="osmF"
                     /locus_tag="KLH32_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBE0138270.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46124..47281
                     /gene="yehY"
                     /locus_tag="KLH32_001385"
     CDS             46124..47281
                     /gene="yehY"
                     /locus_tag="KLH32_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBE0138271.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47274..48200
                     /gene="yehX"
                     /locus_tag="KLH32_001386"
     CDS             47274..48200
                     /gene="yehX"
                     /locus_tag="KLH32_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBE0138272.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48205..48936
                     /gene="yehW"
                     /locus_tag="KLH32_001387"
     CDS             48205..48936
                     /gene="yehW"
                     /locus_tag="KLH32_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBE0138273.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48917..49024)
                     /locus_tag="KLH32_001388"
     CDS             complement(48917..49024)
                     /locus_tag="KLH32_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBE0138274.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49084..49536)
                     /locus_tag="KLH32_001389"
                     /pseudo
     CDS             complement(49084..49536)
                     /locus_tag="KLH32_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11959 a        12201 c        13310 g        12066 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggctag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaggac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtagtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgcgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacaca gaatgtctga
     6661 attaaaaatc tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ctaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 attcatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg cagatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtatgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagcaggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aatatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacacaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac tctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc gggtctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatct
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct caggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcgact
    34921 gcgacgcagt gatgattggt cgcggggcgt tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa gggataagcc
    35401 gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg gggcggcggc
    35461 gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat gcacatgaca
    35521 ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg atgacattaa
    35581 ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa tcaggaaaat
    35641 aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga gtgctgaata
    35701 agtataggaa aaaacgaagt cctttgccgt tatccataaa cgattaagat aaatcggaag
    35761 atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac tgatgtattg
    35821 ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc
    35881 tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt
    35941 actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa gccagcaaac
    36001 gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt ggccagacag
    36061 ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca atgcactaca
    36121 gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag cccaggcgaa
    36181 ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg agcggcaaaa
    36241 aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg caggtacgac
    36301 cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc tcgatatctg
    36361 gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac gggcggcgga
    36421 acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact gggagtggca
    36481 aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga acaccattat
    36541 cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg gtgtggaaac
    36601 cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga aaatgaaaat
    36661 tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga agcttaaacc
    36721 ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact ccttactggc
    36781 ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa gcactattga
    36841 tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc aacaggatgc
    36901 gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct
    36961 gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg caaaagccga
    37021 aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga atgacgtggc
    37081 gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg cgcaaattga
    37141 gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc
    37201 tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt
    37261 attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg gcgggggtta
    37321 caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa gtggagaact
    37381 cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt
    37441 tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat gaagaagggg
    37501 caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc gtgcaactgg
    37561 atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag cgatttgggc
    37621 gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt cttgatatgg
    37681 cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt ttatgctcgc
    37741 aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc aacattacgt
    37801 cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct aaacatgcgc
    37861 tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt ttggtgaacg
    37921 cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc gacgtgaagc
    37981 ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag attgccagcc
    38041 tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg ttgatagtgg
    38101 atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg
    38161 gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat ggaacacccg
    38221 gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga ttatccggct
    38281 ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc acgaccagaa
    38341 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    38401 gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat
    38461 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatca
    38521 cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    38581 gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac aacttgccgc
    38641 caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca agcgccacag
    38701 aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    38761 tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca tattgtgaga
    38821 tgagtgtatt gagagccata ggccagtttg tctccctttg gttgcctgta agcatacacc
    38881 ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta catggatgaa
    38941 caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac tcacaacaag
    39001 ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa atgcaggtga
    39061 ttaatcgcga aaacgaaacg atttctcacc attataccca ccacgttttg ctgatggcgg
    39121 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    39181 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    39241 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgcaattacc
    39301 cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    39361 ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg
    39421 tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc ctgaatatca
    39481 acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg ctggtactgg
    39541 aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    39601 ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact cacgattctc
    39661 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    39721 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    39781 tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    39841 tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg gtagcgcgat
    39901 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc tggtgcgtcc
    39961 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    40021 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    40081 cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    40141 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    40201 taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    40261 taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc
    40321 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    40381 ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt atcgcgataa
    40441 ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt gtctggtgat
    40501 gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    40561 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    40621 tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    40681 gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat ccgtaatttt
    40741 tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg attcatgcta
    40801 ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt atacaaatga
    40861 ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt
    40921 tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg gaagacatag
    40981 cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa
    41041 tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg
    41101 acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt
    41161 aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc ggcctgtttg
    41221 aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat ttttaacagc
    41281 agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac
    41341 aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa tttcactttc
    41401 tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt gtcggtgcct
    41461 aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc
    41521 cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat atgacggcgg
    41581 atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc
    41641 gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat
    41701 tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga
    41761 acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct
    41821 ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc cagatcaatc
    41881 cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa taacgacatt
    41941 tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg
    42001 atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg tcccagcggt
    42061 ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac ctgttcgccc
    42121 ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca
    42181 tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga
    42241 ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca
    42301 gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc
    42361 gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa
    42421 aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa
    42481 aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat
    42541 tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt
    42601 tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc
    42661 tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg gatgatttat
    42721 tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat ttgctcaaga
    42781 aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc ccggataatc
    42841 cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt ttcaacaccg
    42901 taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa
    42961 ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc
    43021 tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt
    43081 atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg
    43141 atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acctcaataa
    43201 tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga
    43261 tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca
    43321 ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc
    43381 tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc acgccagcca
    43441 cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca
    43501 ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg
    43561 aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc gacgagtact
    43621 actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca gagctggacg
    43681 atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca
    43741 gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc
    43801 gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa
    43861 cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg gacagtaaac
    43921 gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg accgtactga
    43981 ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg
    44041 ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg
    44101 atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa caatctgatg
    44161 tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc agccggaccg
    44221 atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc accggtaaac
    44281 cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg
    44341 ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg
    44401 tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg cgttctgtcg
    44461 ggcagatccc ggtgtactac agccatctga acaccggtcg tccgtataat gccgacaagc
    44521 cgaacaaata cacttcgcgt tattttgatg aagctaacgg agcgctttat ccgttcggct
    44581 atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga
    44641 agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg
    44701 ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac
    44761 agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact gtcagcttcc
    44821 cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg
    44881 gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt
    44941 tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta
    45001 acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca tccggcagga
    45061 aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga atccttccgc
    45121 tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa aacaacagta
    45181 aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac tggttatgtt
    45241 ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga
    45301 taccgaaggc gcgctgctcg gcaacatcat ctttcaggta ctcgaaagcc acggcgtacc
    45361 gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga ttacttccgg
    45421 tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta aagatgaaaa
    45481 cgatgcagcg tggaaaaatg ccgggcaggg ctacgagaaa gtcaaaaagc tcgatgcgga
    45541 gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga ctatcgccgt
    45601 gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gccgacctga gtcgttatct
    45661 gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac gcgccgatgc
    45721 gttacccacg tttgaaaaag cctatggctt taagctcggt caggatcagt tgctgtcact
    45781 ggctggtggc gacacggcgg tgacgattaa agccgctgcc cagcaaacct ctggcgttaa
    45841 tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc aaaccttaag
    45901 cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg aatcggtgtt
    45961 gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc tcgatgcaaa
    46021 aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca aaaaagtggc
    46081 tgccgactac ctgaaacaaa aagggtgggc gaagtaattt cccgtgactt atctacgtat
    46141 taatcctgtt ctggcgctgc tgttgttgct gacggcaatc gcagcggcgc tgccgtttat
    46201 cagttacgcg cctaatcgtt tagtctcggg tgaagggctt catctctggc aactgtggcc
    46261 gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct gttttgtccc
    46321 cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg tattgttggt
    46381 gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc tggcgcgtac
    46441 cagcctcggc agaggtttct ggctggctgc ggcgctggca ttgctggcct gtagcgatgc
    46501 catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc agattgccat
    46561 tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa tgaaagaata
    46621 cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt tgctgtttgg
    46681 tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct acttttccac
    46741 tgcgcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc cttcggtggc
    46801 gctctttggc ctgttgattg cgccgcttgc cgcgctggtt acggcctttc cgtggctggg
    46861 gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg tgctctatgc
    46921 cttgctgccg ctggtgcgag gcgtggtagt cggtttgaac cagatcccgc gcgatgtgct
    46981 ggagagcgcc agagcgatgg ggatgagcgg ggcgcagcgt ttcctgcatg ttcagttacc
    47041 gctggcgtta ctggtatttc tgcgcagcct gcgggtggtg atggtgcaaa ctgtaggtat
    47101 ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt tccaggggct
    47161 gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg ttctggcggt
    47221 gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac gtaatgattg
    47281 aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat ctcaatctca
    47341 attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc aaatccacca
    47401 ccctgaaaat gattaaccgc ctggtggagc atgacagcgg agtgatccgc tttgccggag
    47461 aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat gccattcaat
    47521 ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg ccgcaattac
    47581 aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg ctactggggc
    47641 tggagtcaaa tttgcgtgag cgttatccgc atcagctttc cggtggtcag cagcaacgtg
    47701 tgggagtggc gcgtgcactg gctgccgatc cgcaagtctt actgatggat gaaccttttg
    47761 gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc attcaccgtt
    47821 tgctggggcg cactattgtg ctggtcactc atgatattga tgaggcgcta cggctggcag
    47881 aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg ctgacgatgc
    47941 tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa ctgggggtgc
    48001 acctgctttc gttacgtagc gtggcggatt acgtgcgtcg cgaagaacgg gcagaaggtg
    48061 aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc gcgcggggat
    48121 gcgaggtgct gccggtggtg aacacgcagg gccagccttg cggcacgctg cattttcagg
    48181 atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt ctggctcatt
    48241 gctctttttg tggcgctgat tttctggctg ccttacagcc agccgctgtt tgctgccttg
    48301 ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct ggcactggct
    48361 catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggcac aggtgccgga
    48421 attgctgtta ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac tattgccgcc
    48481 gttggacaga cttttccgcc tgtcgcagtg ctggcgattg ccgttccggt gatcggcttt
    48541 ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccat cctgcaggtg
    48601 acacttgccg ggctgggagc gattgatccc agcgtgacag aagttgcgaa aggtatggga
    48661 atgagtcgag gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc ggtgattctg
    48721 gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc ctcaacggta
    48781 ggggccagta cgctgggtac gcccatcatc atcgggctta gcggatttaa taccgcgtat
    48841 gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg cctgtttgaa
    48901 aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg agcatgacgc
    48961 caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt ttagctatgc
    49021 gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt tgttaacgaa
    49081 tcattaaata ccgagtggga aaatatcatg gccttgttct tgccaactgg tgagttgctg
    49141 ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc cttcgaatag
    49201 ttcagggcgt agttgattga gcgagtgggc gagcgaggac atcaatgcgc catccttgtt
    49261 gactggcaat ccagccctcc agccacagac gggtggtatc ctgaatattc cagccaacca
    49321 ccagcgcatc tttaccctgt tttttacgtg ccgaagccag acaaatggcg atgtagttga
    49381 tcagtacgcc gtcgaggatc gccagcagcg cctggagagt cggttgttgg cactgaagcc
    49441 gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg ggcggggtaa tcctgaccgc
    49501 gctctttgat ccacgttcgc aggctatgta gattgc
//
LOCUS       DAEEDS010000023        48607 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEEDS010000023 DAEEDS010000000
VERSION     DAEEDS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48607
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.023"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            30..1280
                     /locus_tag="KLH32_001390"
     CDS             30..1280
                     /locus_tag="KLH32_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0138275.1"
                     /translation="MSKLESLPEFELPEITDNELLVSVISDSVCLSTWKAALLGSEHK
                     RVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEY
                     FGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHR
                     MGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLA
                     ASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLN
                     FFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHI
                     GGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETH
                     GIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1277..2242
                     /gene="yggF"
                     /locus_tag="KLH32_001391"
     CDS             1277..2242
                     /gene="yggF"
                     /locus_tag="KLH32_001391"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBE0138276.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2264..2773
                     /gene="fumE"
                     /locus_tag="KLH32_001392"
     CDS             2264..2773
                     /gene="fumE"
                     /locus_tag="KLH32_001392"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBE0138277.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNETLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2770..3483
                     /locus_tag="KLH32_001393"
     CDS             2770..3483
                     /locus_tag="KLH32_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBE0138278.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3455..4137)
                     /locus_tag="KLH32_001394"
                     /pseudo
     CDS             complement(3455..4137)
                     /locus_tag="KLH32_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4131..4808)
                     /locus_tag="KLH32_001395"
     CDS             complement(4131..4808)
                     /locus_tag="KLH32_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE0138279.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4796..5179)
                     /locus_tag="KLH32_001396"
     CDS             complement(4796..5179)
                     /locus_tag="KLH32_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBE0138280.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5195..5503)
                     /locus_tag="KLH32_001397"
     CDS             complement(5195..5503)
                     /locus_tag="KLH32_001397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138281.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5504..6082)
                     /locus_tag="KLH32_001398"
     CDS             complement(5504..6082)
                     /locus_tag="KLH32_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE0138282.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6105..6536)
                     /locus_tag="KLH32_001399"
                     /pseudo
     CDS             complement(6105..6536)
                     /locus_tag="KLH32_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6908..7927
                     /gene="epd"
                     /locus_tag="KLH32_001400"
     CDS             6908..7927
                     /gene="epd"
                     /locus_tag="KLH32_001400"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBE0138283.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7977..9140
                     /gene="pgk"
                     /locus_tag="KLH32_001401"
     CDS             7977..9140
                     /gene="pgk"
                     /locus_tag="KLH32_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBE0138284.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9355..10434
                     /gene="fbaA"
                     /locus_tag="KLH32_001402"
     CDS             9355..10434
                     /gene="fbaA"
                     /locus_tag="KLH32_001402"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBE0138285.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10625..11485
                     /gene="mscS"
                     /locus_tag="KLH32_001403"
     CDS             10625..11485
                     /gene="mscS"
                     /locus_tag="KLH32_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBE0138286.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11624..12259
                     /gene="argO"
                     /locus_tag="KLH32_001404"
     CDS             11624..12259
                     /gene="argO"
                     /locus_tag="KLH32_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBE0138287.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12352..13092
                     /locus_tag="KLH32_001405"
     CDS             12352..13092
                     /locus_tag="KLH32_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBE0138288.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13259..14209
                     /locus_tag="KLH32_001406"
     CDS             13259..14209
                     /locus_tag="KLH32_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0138289.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KLH32_001407"
     CDS             complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KLH32_001407"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBE0138290.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KLH32_001408"
     CDS             complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KLH32_001408"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBE0138291.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KLH32_001409"
     CDS             complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KLH32_001409"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBE0138292.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KLH32_001410"
     CDS             complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KLH32_001410"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBE0138293.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KLH32_001411"
     CDS             complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KLH32_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBE0138294.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21052..21711
                     /gene="rpiA"
                     /locus_tag="KLH32_001412"
     CDS             21052..21711
                     /gene="rpiA"
                     /locus_tag="KLH32_001412"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBE0138295.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21967..23199
                     /gene="serA"
                     /locus_tag="KLH32_001413"
     CDS             21967..23199
                     /gene="serA"
                     /locus_tag="KLH32_001413"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBE0138296.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23428..23487
                     /gene="ibsC"
                     /locus_tag="KLH32_001414"
     CDS             23428..23487
                     /gene="ibsC"
                     /locus_tag="KLH32_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBE0138297.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23820..23879
                     /locus_tag="KLH32_001415"
     CDS             23820..23879
                     /locus_tag="KLH32_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0138298.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23980..24582)
                     /locus_tag="KLH32_001416"
     CDS             complement(23980..24582)
                     /locus_tag="KLH32_001416"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBE0138299.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24603..24786)
                     /gene="ssrS"
                     /locus_tag="KLH32_001417"
     ncRNA           complement(24603..24786)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KLH32_001417"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KLH32_001418"
     CDS             complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KLH32_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBE0138300.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25325..25903
                     /gene="ygfB"
                     /locus_tag="KLH32_001419"
     CDS             25325..25903
                     /gene="ygfB"
                     /locus_tag="KLH32_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBE0138301.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25929..27254
                     /gene="pepP"
                     /locus_tag="KLH32_001420"
     CDS             25929..27254
                     /gene="pepP"
                     /locus_tag="KLH32_001420"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBE0138302.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27251..28429
                     /gene="ubiH"
                     /locus_tag="KLH32_001421"
                     /gene_synonym="visB"
     CDS             27251..28429
                     /gene="ubiH"
                     /locus_tag="KLH32_001421"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBE0138303.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28452..29654
                     /gene="ubiI"
                     /locus_tag="KLH32_001422"
                     /gene_synonym="visC"
     CDS             28452..29654
                     /gene="ubiI"
                     /locus_tag="KLH32_001422"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBE0138304.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29728..29811)
                     /locus_tag="KLH32_001423"
     CDS             complement(29728..29811)
                     /locus_tag="KLH32_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138305.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30102..31196
                     /gene="gcvT"
                     /locus_tag="KLH32_001424"
     CDS             30102..31196
                     /gene="gcvT"
                     /locus_tag="KLH32_001424"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBE0138306.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31220..31609
                     /gene="gcvH"
                     /locus_tag="KLH32_001425"
     CDS             31220..31609
                     /gene="gcvH"
                     /locus_tag="KLH32_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBE0138307.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31728..34601
                     /gene="gcvP"
                     /locus_tag="KLH32_001426"
     CDS             31728..34601
                     /gene="gcvP"
                     /locus_tag="KLH32_001426"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBE0138308.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34868..35610
                     /locus_tag="KLH32_001427"
                     /pseudo
     CDS             34868..35610
                     /locus_tag="KLH32_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KLH32_001428"
     CDS             complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KLH32_001428"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBE0138309.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37145..37455
                     /locus_tag="KLH32_001429"
                     /pseudo
     CDS             37145..37455
                     /locus_tag="KLH32_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37619..38278
                     /locus_tag="KLH32_001430"
     CDS             37619..38278
                     /locus_tag="KLH32_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBE0138310.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KLH32_001431"
     CDS             complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KLH32_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBE0138311.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFKHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39697..39963
                     /gene="sdhE"
                     /locus_tag="KLH32_001432"
     CDS             39697..39963
                     /gene="sdhE"
                     /locus_tag="KLH32_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBE0138312.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39944..40351
                     /locus_tag="KLH32_001433"
     CDS             39944..40351
                     /locus_tag="KLH32_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBE0138313.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KLH32_001434"
     CDS             complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KLH32_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBE0138314.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            41024..41920
                     /gene="xerD"
                     /locus_tag="KLH32_001435"
     CDS             41024..41920
                     /gene="xerD"
                     /locus_tag="KLH32_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBE0138315.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41945..42655
                     /gene="dsbC"
                     /locus_tag="KLH32_001436"
     CDS             41945..42655
                     /gene="dsbC"
                     /locus_tag="KLH32_001436"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBE0138316.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42661..44394
                     /gene="recJ"
                     /locus_tag="KLH32_001437"
     CDS             42661..44394
                     /gene="recJ"
                     /locus_tag="KLH32_001437"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBE0138317.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44485..45583
                     /gene="prfB"
                     /locus_tag="KLH32_001438"
     CDS             join(44485..44559,44561..45583)
                     /gene="prfB"
                     /locus_tag="KLH32_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBE0138318.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45593..47110
                     /gene="lysS"
                     /locus_tag="KLH32_001439"
     CDS             45593..47110
                     /gene="lysS"
                     /locus_tag="KLH32_001439"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBE0138319.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KLH32_001440"
     CDS             complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KLH32_001440"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBE0138320.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47756..47827
                     /locus_tag="KLH32_001441"
     CDS             47756..47827
                     /locus_tag="KLH32_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138321.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47824..47949)
                     /locus_tag="KLH32_001442"
     CDS             complement(47824..47949)
                     /locus_tag="KLH32_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138322.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KLH32_001443"
     CDS             complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KLH32_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBE0138323.1"
                     /translation="IIVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTF
                     NSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAG
                     LVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLL
                     HSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE
                     "
BASE COUNT        11666 a        12185 c        13120 g        11636 t
ORIGIN      
        1 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta cccgaatttg
       61 aactgccaga aattaccgat aatgaactgt tagtgagtgt aatttctgac agcgtctgtt
      121 tatcgacctg gaaagcggcg ttactcggta gtgaacataa acgcgtaccc gacgatttag
      181 aaaatcatcc ggtcattacc gggcatgaat gtgccggggt tattgtcgaa gtgggtaaaa
      241 atctcactgg caaatataaa aaaggccagc gttttgtatt gcaaccggcg atggggttac
      301 caagcggata ttcagcgggc tacagctacg aatattttgg cggcaatgcc acttatatga
      361 ttattcccga aatcgccatt aatttgggct gcgtattacc gtatcacggc tcttattttg
      421 ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat gccaattatc
      481 acaccacgca atatgtttat gagcatcgca tgggcgtcaa acctggcggc aatattgcac
      541 tgctggcgtg tgcgggtccg atgggcattg gcgctatcga ttacgccatt aacggtggca
      601 tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa acgcctggcg caggtgcaga
      661 agctgctgcc ggtggatctg gcggccagta aaggcattga gctagtgtat gtgaatacca
      721 aagggatgag cgatcctatc cagatgctgc gggcgctgac aggagatgtc gggttcgatg
      781 acatttttgt ttatgcggcg gtgcctgctg tcgttgagat ggctgacgaa ttactggcgg
      841 aagatggctg tctgaacttc tttgccgggc tgacggataa aaacttcaaa gtgccgttta
      901 atttctacaa cgtccattac aacagcacgc acgtagtcgg tacatccggc ggttcaacgg
      961 acgacatgaa agaggcgatt gcccttagcg ccacaggaca gttacagccg tcgtttatgg
     1021 tgacccatat tggtggcctg gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc
     1081 ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctcactgcc attgccgatt
     1141 ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagtt ggcgcggctg gttgaggaaa
     1201 cgcacggcat ctggaatgaa caggccgaga aatatctgct ggcacaattt ggcgttgata
     1261 tcggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc
     1321 agcgttggct gcctggccgc acaccggatg tggcgacaaa aataaaattg atggcctggc
     1381 ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt cgtggacgag tggttatcgg
     1441 cgaaggagaa attgaccatg cgccgatgct gtggattggc gaagaggtgg gcaaaggcga
     1501 tgggccagaa gtcgatatcg cggttgatcc cattgaaggc acgcggatgg tggcgatggg
     1561 gcagagcaat gcgctggcgg tcatggcttt cgctcctcga gatagcctgt tacatgcgcc
     1621 cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga ttgatctgtc
     1681 tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa
     1741 gctgcgcatg gttacgctgg ataaaccgcg tctgagtgcc gcgattgaag aagcaacaca
     1801 gctgggcgtg aaggtttttg ccctgccgga tggcgatgtc gccgccagcg tgctgacctg
     1861 ctggcaggat aatccttacg atgtgatgta caccatcggc ggtgcgccag aaggcgtgat
     1921 ttctgcctgt gccgttaaag cgttaggcgg ggatatgcag gccgagctga ttgacttttg
     1981 tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc gcaagcgttg
     2041 taaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg tgagggggaa
     2101 cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca
     2161 gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa
     2221 tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga
     2281 agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta tgaaaactgc
     2341 gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga
     2401 tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga
     2461 tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtatgc
     2521 cgatatcacg cacttttccc agtactggca ttacctgaac gaacaggatg aaacgcccgg
     2581 atttgccgat gatataacct gggattttat cagcaatgtc aacagtataa ctcgcaatga
     2641 gacgctctac gatgcgttaa aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc
     2701 gcgttttagc ggaatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga
     2761 attaacgccg tgaaaattga attaacggtg aatgggctga acgtgcaagc gcagtaccat
     2821 gatgaagaga tagaacgcgt gcataaactg ttgctgcgta tgctggcggc cttacagacg
     2881 gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac gggtaaatct
     2941 accctaacca ccttctggga atatctcgcg caacaagacc cggagctgcc cgctattcag
     3001 gcgctcccga tggatggttt tcaccattac aatagctggc tggacgcgca tcaattgcgc
     3061 cccttcaaag gcgcaccaga gacatttgac gttgcgaaac tggcggaaaa tctgcgccag
     3121 gtggtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga tcctgttgaa
     3181 gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt gttgctggac
     3241 gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat caacgcgcca
     3301 gctgctgcgt tgcgggagcg tctggtgggg cgcaagctgg cgggaggggt atcgctggca
     3361 gatgcagaag ccttttatga ccgtaccgac gggccgaatg ttcgccgggt gctggaagag
     3421 agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg tctggtggat
     3481 taacgttcct gattacacca tcttccagcc gcaccacacg agagaaatga cgccgggtaa
     3541 atgcggcgtc atgactgatt gctaccacgc tcgtgcctcg ctggcggcac ttctccagcc
     3601 agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg tccagcagta
     3661 atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc tgcgcactat
     3721 gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg acaacattgc agggcagcat
     3781 gggtacgttg agtgatttca tccgccgggc atttttgcag tttcaaacca aatgcgatct
     3841 cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc ctgaaacaga acagtacgcc
     3901 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     3961 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
     4021 gccattgtca ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     4081 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     4141 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     4201 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     4261 tgcaacagca tttcgctggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaag
     4321 agctttggct gcatcgcgat ggcgcaggcg atgatcacgc gctgtgtctc tccaccggag
     4381 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     4441 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     4501 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     4561 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     4621 gtgcctgtta tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     4681 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     4741 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     4801 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     4861 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     4921 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     4981 gcggaataat taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca
     5041 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     5101 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     5161 cgcgaataaa acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg
     5221 ccagaatcag caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
     5281 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     5341 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     5401 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     5461 aggccgccaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     5521 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     5581 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     5641 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     5701 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     5761 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     5821 cggagaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca
     5881 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     5941 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     6001 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     6061 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     6121 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     6181 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     6241 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     6301 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     6361 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca
     6421 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     6481 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga
     6541 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     6601 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     6661 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     6721 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     6781 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     6841 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     6901 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     6961 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     7021 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     7081 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     7141 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     7201 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     7261 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     7321 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     7381 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     7441 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     7501 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     7561 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     7621 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     7681 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     7741 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     7801 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     7861 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     7921 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     7981 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     8041 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     8101 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     8161 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     8221 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     8281 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     8341 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     8401 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     8461 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     8521 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     8581 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     8641 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     8701 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     8761 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     8821 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     8881 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     8941 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     9001 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     9061 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     9121 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg
     9181 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     9241 gagtgaatcc ccaggagctt acataagtaa gtgactgggg ggagcgaacg cagacgcagc
     9301 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     9361 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     9421 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     9481 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     9541 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     9601 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     9661 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     9721 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     9781 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     9841 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     9901 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     9961 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
    10021 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    10081 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    10141 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    10201 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    10261 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    10321 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    10381 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    10441 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
    10501 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
    10561 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
    10621 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
    10681 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
    10741 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
    10801 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
    10861 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
    10921 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
    10981 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
    11041 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
    11101 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
    11161 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
    11221 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
    11281 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
    11341 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
    11401 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
    11461 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
    11521 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
    11581 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
    11641 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
    11701 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
    11761 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
    11821 gtggttgcta gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
    11881 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
    11941 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
    12001 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
    12061 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
    12121 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
    12181 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
    12241 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
    12301 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
    12361 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
    12421 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
    12481 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
    12541 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
    12601 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
    12661 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
    12721 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
    12781 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
    12841 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
    12901 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
    12961 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
    13021 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
    13081 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
    13141 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
    13201 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
    13261 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
    13321 tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
    13381 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
    13441 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
    13501 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
    13561 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
    13621 gactgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
    13681 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
    13741 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
    13801 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
    13861 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
    13921 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
    13981 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
    14041 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
    14101 taccgccacg atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc
    14161 atcgagccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
    14221 atatgtccgc cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt
    14281 gcacaattat caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg
    14341 atcccttgtt cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac
    14401 attggcacga cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac
    14461 agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg
    14521 gctacgtgcg ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg
    14581 atgacgccca gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca
    14641 atgcggctgg caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt
    14701 gtcaggctgg aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat
    14761 tcctgtagca cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt
    14821 gccattaccg cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga
    14881 atacgcccat gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg
    14941 cacatgggat tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg
    15001 gcgacaatct ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg
    15061 aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct
    15121 gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc
    15181 caggtcggcg cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc
    15241 gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc
    15301 acttcgctca aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa
    15361 ctggacgatg tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg
    15421 tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc
    15481 tcatgctgtt cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct
    15541 ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg
    15601 gcggtcatcc ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac
    15661 caacgaaatt aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc
    15721 tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt
    15781 gtgcttcgcc cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct
    15841 cagagatgtg gtgcgccatt tgtaaggtga aatcttccac ttccacaaca tggttgagga
    15901 tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agctctttga
    15961 caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc
    16021 cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac
    16081 tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt
    16141 tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat
    16201 cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggatccac
    16261 ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt aacgcctgca
    16321 taagatcatc aataaagact ttacttaagg cattaagttt tcgtccatag ttaaactcaa
    16381 tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata aaaattcctt
    16441 taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag
    16501 agcgtattgt ttttgaccgc taaaagcgtc tggtggtaat agcgatcgaa atcttcattc
    16561 gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt
    16621 tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg
    16681 gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag accccgtggc
    16741 tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca
    16801 atggcgacat tggtatggtt atcgccatcg tctttgttga taacgagcag atcagccact
    16861 tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag
    16921 ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgacgcc aaccgtttcg
    16981 acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg tgctcgctga
    17041 ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc
    17101 gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg
    17161 ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat gccaaaggct
    17221 tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta
    17281 ccacagaacg gcataatagc gtcaagcagc tgtgtactta gcgcctgatg acgtgggtga
    17341 cggctttcca ccagcgtcat ggcttgggcg agcgtggcac gctcaccctg acgtaagcgg
    17401 cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac
    17461 gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc
    17521 ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttc
    17581 gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc
    17641 cagtgaggat gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc
    17701 aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc
    17761 gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg
    17821 gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga
    17881 ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg
    17941 caccagataa cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg
    18001 aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt
    18061 cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag
    18121 cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc
    18181 ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag
    18241 cgactgttcg cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc
    18301 tttcgccatg ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat
    18361 ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgtggcagag
    18421 ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt
    18481 aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt
    18541 cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag
    18601 gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc
    18661 aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat
    18721 aaacagatcc atgccgatac cgaagaataa cgacaggcga ggagcgaagt catcaatttt
    18781 cagtccggca gagattgctg ctttgatgta ctcaatccca tcggcgatcg taaatgctac
    18841 ctgctgcacg cagttggcac ccgcttcccc catgtggtaa ccgctgatac tgatggtatt
    18901 aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg
    18961 ttttggtggg taaatatagg tgttgcggca gaggtactct ttgagaatat cgttttgaat
    19021 ggtgccggtc agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc
    19081 cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat
    19141 ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac
    19201 gtcacccgcc acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc
    19261 aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt
    19321 tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc
    19381 acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc
    19441 gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag
    19501 cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg
    19561 ctcgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg
    19621 tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc
    19681 cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa
    19741 cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga
    19801 ccataatcga gtaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg
    19861 tgccagtaga gcatccgtcg ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg
    19921 gccagctctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca
    19981 agttgtacga acgcttctga agaattaacg atatggcagg gtacgctgcc aggaggaaga
    20041 tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact
    20101 ggcgctttca ataatgccga acgcgttacg ccgttaggaa aatatttttc ggcaaacggt
    20161 tttgagctga cgaacagata gtcgagcgca ccgagtttat cgacaagaca actcggcagc
    20221 gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagta
    20281 cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc
    20341 agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc
    20401 ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc
    20461 agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac
    20521 agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta
    20581 atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc
    20641 tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt
    20701 cctgcactat gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca
    20761 tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta ttgcatttcg
    20821 cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct ctgttcgcac
    20881 cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg
    20941 ccattgcgcg atattttatg gatgagttaa ccacccagct tgccaacggg gtctgaatcg
    21001 ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag
    21061 gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggtaccatt
    21121 gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg cacgatgaaa
    21181 ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc
    21241 ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca
    21301 gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa
    21361 aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc caagcaggtt
    21421 gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg
    21481 gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat
    21541 aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa
    21601 aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg
    21661 gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg
    21721 ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt
    21781 accaggcaat tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat
    21841 tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta
    21901 ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt
    21961 gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc
    22021 gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac
    22081 aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc
    22141 ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct
    22201 attggctgtt tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg
    22261 atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc
    22321 gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg
    22381 tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc
    22441 tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac
    22501 ttttatgata ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct
    22561 gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa
    22621 aatatgatgg gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc
    22681 tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg
    22741 gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc
    22801 tctccactgt gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag
    22861 gaagcgcagg agaatatcgg cttggaagtt gcgggtaaat tgatcaagta ttctgacaat
    22921 ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt
    22981 cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc
    23041 gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat
    23101 gtagttattg atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa
    23161 gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa
    23221 cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg
    23281 cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt
    23341 tatttaatgg ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc
    23401 gaagcgaggg cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    23461 ttactgataa gtttcggagc ttattaacag tcagtctcaa gggaggagtc atcctccctt
    23521 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg
    23581 catcgccctg aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata
    23641 gatccttttt cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga
    23701 aaagatttaa aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg
    23761 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta
    23821 tgatgcgact tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac
    23881 agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga
    23941 atcaacgatg tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga
    24001 cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa
    24061 ctgacaatca tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt
    24121 ccgatcataa aaaccaccgc ccattcccag gcgctgaccg tactcatcaa aggcgaccag
    24181 cggtgtgatc agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg
    24241 ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag
    24301 caaattaccg gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca
    24361 gagttgttct attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac
    24421 cgtatgcgcc atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat
    24481 ttccagctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc
    24541 ttgtcgggat aatgtcagtg ggagctctgg tagttgcgtc atggctggtg ccagataaga
    24601 agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat
    24661 gtgtcgtcgc agttttaagg cttctcggac gaaccgagca tgctcaccaa ccgcggagcg
    24721 ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag
    24781 agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt
    24841 tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc
    24901 agcatccgaa tacgctgttc catacttgcc gcgtagtcac gagtctttgc tttttcttgc
    24961 gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact
    25021 ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac
    25081 gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg
    25141 acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac
    25201 ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc
    25261 tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat
    25321 gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca
    25381 acaagggacg ggtctgaccc ctgccgagat gcatggttta atcagtggga tgatatgtgg
    25441 cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg aaggcatggc
    25501 tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct
    25561 gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt
    25621 cgatcgggct gatgcactgg caggttgggt caatcacttc ctgcttggtc ttggcgttac
    25681 gcaaccgaag ctggacaaag tgaccggcga aaccggtgaa gccatcgacg atctgcgtaa
    25741 catcgcgcag ctgggctacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga
    25801 agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc
    25861 gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg
    25921 agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg
    25981 agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg
    26041 ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg
    26101 aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt
    26161 ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgctta ggccaggatg
    26221 ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc aatcagcaac
    26281 tttatcaact acttaacggc ctggatgtgg tttaccatgc ccagggcgaa tatgcatatg
    26341 ctgatgaaat cgtgaacagt gcgctggaaa aactgcgtaa aggttcgcgg caaaatctca
    26401 ccgcaccggc aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc ctgttcaaat
    26461 cgccagaaga gattgccgta ctccgccgcg cgggagaaat caccgccatg gcacatacac
    26521 gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc gaaattcacc
    26581 acgaatttaa ccgccacggt gcgcgctatc cttcctataa caccattgta ggcagcggtg
    26641 aaaacggctg cattctgcac tacaccgaaa acgagagtga actgcgcgac ggtgacctgg
    26701 tgttgattga cgcgggttgt gaatacaaag gttacgcggg cgatattacc cgcaccttcc
    26761 cggtcaacgg caaattcacc caggcccagc gtgaaatcta cgacattgtg ctggagtctc
    26821 tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tctggaagtc actggtgaag
    26881 tggtgcgcat catggttagc ggcctggtaa aactcggcat cctgaaaggt aatgttgatg
    26941 aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag
    27001 gactggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg
    27061 ggatggtact gaccgtagag cctggtctgt atatcgcgcc ggatgcggat gtgccagaac
    27121 aatatcgcgg tatcggcatt cgcattgaag acgacattgt gattaccgaa accggcaacg
    27181 aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggctgctg
    27241 cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct
    27301 ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag cgactgcgcc
    27361 agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg cgggtacctg
    27421 tcagcaactg gcgcgcatcg gcgtctggca atctctggct gattgcgcaa ccgccatcac
    27481 taccgtgcat gtcagcgatc gtggtcatgc tggattcgtc accctcgccg cagaagatta
    27541 ccaactggcg gcgctggggc aggttgtcga attgcataat gtcgggcaac ggctgtttgc
    27601 attgctgcga aaagcacctg gcgtaatgct gcattgcccc gatcgcgtag ctaacgttgc
    27661 ccgtactcag agtcatgttg aagtgacgct ggagagcggc gagatgctga cgggccgcgt
    27721 gctgatagcc gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgattggca
    27781 gcaggagcct tacgaacaac tggcagtgat tgccaacgtt gctacttctg ttgcgcacga
    27841 aggccgcgct tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga
    27901 cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg
    27961 gagcgacgag aagttttgcc gtgaactcca gtctgccttt ggctggcgac tggggcaaat
    28021 tacccacgcc ggtaaacgca gtgcttatcc gctggcatta acccgcgccg ccagaccgat
    28081 tacccatcgc actgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca
    28141 agggtttaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga ctcaggcgca
    28201 tgagcgcgga gaagacatcg gtgattacgg catattgtgc cgctatcagc agcgtcgaca
    28261 gagcgatcgc gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg
    28321 ttgggcaccg ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc
    28381 ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta
    28441 aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg gtggggctgg
    28501 cggttgcctg cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac
    28561 cggaacctct ggcggtagat gcactaccac aactgcgcgt ttcggctatc aatgccgcca
    28621 gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctcgctcgt agggccagtt
    28681 gttatcacgg catggaagtg tgggacaaag acagttttgg tcacatctcg tttgacgatc
    28741 aaagcatggg ctatagccat cttgggcaca tcgttgaaaa ctcagtgatt cactacgcgc
    28801 tgtggaacaa agcgcagcag tcgtcagata ttactctgtt ggcccccaca gaattacagc
    28861 aggtcgcctg gggagaaaat gaaaccttcc tgacgctgaa agatggcagc atgttaacgg
    28921 cgcgtctggt gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc
    28981 cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac
    29041 cgcatgatgc ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc
    29101 ttagcgatcc gcatctttgc tcgattgtct ggtcactgtc gccagaggaa gcgcagcgga
    29161 tgcagcaggc aagtgaagac gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc
    29221 ttggcttatg caaggttgag agcgcgcgtc aggtgttccc gctgacgggg cgttatgcgc
    29281 gccagtttgc cgcacaccgt ctggcgctgg tgggcgacgc cgcgcatacc attcacccac
    29341 tggcggggca gggcgttaat ctcggattta tggacgccgc agagctgatt gctgaactga
    29401 aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttacctgcgc cgctatgaac
    29461 gtagccgcaa gcacagcgcg gcgctgatgc tggctggtat gcagggtttt cgcgatctct
    29521 tttccggcgc taatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca
    29581 cgcttcctgg cgttaagccg caacttatcc gccaggcaat gggattaaac gatttgcctg
    29641 aatggctgcg ttaaaaattt ctcctctgtt gtttatttga tacccatcac actttcatct
    29701 cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt
    29761 cgcattatat tttctaatac catgattttt tgatttagtg ttttttgaca tttttttagc
    29821 tcttaatatt gtcttattca aattgacttt ctcatcacat catctttgta tagaaattgg
    29881 tgtatttttt ggttttttat tctgtcgcga tttttgcatt ttttaaccat aagctaatgt
    29941 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
    30001 aacgattgcg ttggggaccg ggagtggctc cgatgctggg ttttgtggtg ataatttcac
    30061 catgaaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    30121 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    30181 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    30241 ttgatgtgtc gcatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    30301 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag aggcaaagcc ctttactcgg
    30361 ggatgttgaa tgcctctggc ggtgtgatag atgatctcat cgtctactac tttactgaag
    30421 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    30481 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    30541 tgcaagggcc gaatgctcag gcaaaagctg ccacactgtt taatgatgct cagcgtcagg
    30601 cggtggaagg gatgaaaccg ttctttggcg tgcaggtggg cgatctgttt attgccacca
    30661 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    30721 tctggcgtgc gctggtggaa gcgggcgtta agccctgcgg tctgggggcg cgtgacacgc
    30781 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    30841 tagccgccaa catgggctgg actatcgcct gggaaccgac agatcgtgac tttatcggtc
    30901 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    30961 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    31021 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    31081 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    31141 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    31201 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    31261 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    31321 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    31381 gggcgatgac tgcgcggttg ccgaatcggt aaaagcggcg tcagacattt atgcgccagt
    31441 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag
    31501 cgaaccgtat gcaggcggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    31561 atcactgctg gatgcgaccg catacgaagc attgttagaa gatgagtaac ggctttattc
    31621 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    31681 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    31741 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    31801 aacagcaaga aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga
    31861 ttgtgccgaa agatattcag cttgcgacac caccgcaggt tggcgcaccg gcgaccgaat
    31921 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    31981 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    32041 atccgggctg gtataccgcg tacactccgt atcagcctga agtctcccag ggccgccttg
    32101 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    32161 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    32221 gcaaactgaa aaatgccaac cgcttcttcg tggcttccaa tgtgcatccg caaacgctgg
    32281 atgtggtccg tactcgtgct gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    32341 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    32401 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    32461 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    32521 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    32581 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    32641 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    32701 ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    32761 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    32821 accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa ctgcgccatg
    32881 cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta ctgacgcgtg
    32941 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg atcacccttg
    33001 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag tgtgctgctg ggcgataacc
    33061 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctatccagc
    33121 ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc tatcacagcg
    33181 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    33241 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    33301 tcacttggac ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    33361 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    33421 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    33481 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    33541 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    33601 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    33661 acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc
    33721 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    33781 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    33841 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    33901 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    33961 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    34021 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    34081 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    34141 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    34201 aaaccggcat cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc
    34261 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    34321 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    34381 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    34441 gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta ttcccggcag
    34501 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    34561 acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat tcgagtattt
    34621 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    34681 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    34741 atttaccctc accccgaccc tctccctgaa agggcgaggg ggaaaagcgt gccaacattg
    34801 aagattgagc cagtttgtta gcaatctcaa agatacgtca acgaattaat ttttctcgga
    34861 aaaacaaatg gctatagcac ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgt
    34921 attactgttg gcgcaagaag ggtatacggt ggcggttaat tatcagcaaa acctccacgc
    34981 ggcgcaggaa gtgatgaact taataatgca agccggtggc aaagcattcg tgctccaggc
    35041 ggatatcagc gacgaaaacc aggtcgttgc gatgtttaca gcaatcgatc agcacgatga
    35101 accgctagca gcgctggtca ataacgccgg gatcttgttt acccagtgca ccgttgaaaa
    35161 ccttaccgca gagcgaatca accgagtact ttccaccaac gtgacgggat attttctctg
    35221 ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt
    35281 caatgtctct tcggtggcct cacggttggg ttcgccaggg gaatatgttg attacgcggc
    35341 atcgaaaggg gcgattgatt cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg
    35401 gatccgcgtt aactgcgtgc ggccagggtt tatttatacc gaaatgcacg ccagcgcggc
    35461 gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca
    35521 gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga
    35581 agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca
    35641 agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct
    35701 ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt
    35761 ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag
    35821 tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt
    35881 ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca
    35941 tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga
    36001 taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc
    36061 cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg
    36121 ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc aagataatcg
    36181 caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt aaatccgcga
    36241 cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac atcggtaaag
    36301 acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acatgagtag
    36361 ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc
    36421 gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg gtacatcgtc
    36481 tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc aaacagcggc
    36541 gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt gactttatgc
    36601 ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa tttacggttg
    36661 gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga gagggttatc
    36721 accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa cttcagccct
    36781 tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc
    36841 cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa
    36901 aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt ggtgatttcg
    36961 cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggtcc tttgccgcct
    37021 ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa gaaatctttc
    37081 ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta caggaaatag
    37141 taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat tctggctggg
    37201 cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt
    37261 cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac cgcaacctca
    37321 accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat gaccctgcct
    37381 gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta tatgattgaa
    37441 tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt taatttcatg
    37501 atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt tcactggaac
    37561 tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg agtaagttat
    37621 ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg ccaacagcgt
    37681 cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac tggttcaggc
    37741 ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg gcggcagtat
    37801 gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac gggcaaaaat
    37861 gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa cctacacgcc
    37921 gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg ttatctggag
    37981 cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca aaattttatc
    38041 tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg aaatggcagt
    38101 taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt attcgctcgg
    38161 ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc acggcttcgt
    38221 gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc aggcgtaatg
    38281 ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac
    38341 cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt tgtgataaag
    38401 gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata aatgcctgat
    38461 gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg
    38521 catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg acgacctgta
    38581 ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta cgacgccagt
    38641 tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg acactacctg
    38701 ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc accatctctt
    38761 gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga ttggtcgcct
    38821 gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct tcaatgttta
    38881 atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca
    38941 tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt ttaaaccata
    39001 gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt tcgctaaaga
    39061 gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt
    39121 ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc agctgcggtt
    39181 cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac agacgtaaat
    39241 tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga tcttctgcca
    39301 tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc gcgccagtaa
    39361 tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg
    39421 taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt gcttaacggc
    39481 gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca ctacgcgatc
    39541 tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg tgaattaatt
    39601 tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa
    39661 tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa caaagcccga
    39721 attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat gccgtttttc
    39781 gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg tctgctggaa
    39841 tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc cgatgcagaa
    39901 ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc tgtggcaatc
    39961 tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg ggctggttgc
    40021 cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga tggtgttact
    40081 ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc gccaggggga
    40141 aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa
    40201 agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg gcggtaaacg
    40261 gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg atttacggcg
    40321 gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat gcgctaagcc
    40381 gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg caccagctct
    40441 gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga cccacgaaca
    40501 gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc gttggccagt
    40561 agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg agcgcatcga
    40621 ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata attttacctt
    40681 caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg atttcaccaa
    40741 aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat ttcggggagt
    40801 cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg attttttctg
    40861 ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc ataacgtaag
    40921 cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta gtcacggcac
    40981 aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac aggagctggc
    41041 acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg aaaatacgtt
    41101 gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac
    41161 gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc tggagggcgg
    41221 gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt tccagtatct
    41281 ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc
    41341 ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac aggcgccatt
    41401 aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt atgctaccgg
    41461 gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc gtcagggcgt
    41521 agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt
    41581 ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat
    41641 tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct tctggcaccg
    41701 cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt
    41761 gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg tggtgcagat
    41821 gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg ctaccgagcg
    41881 tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag
    41941 atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag gctttgctca
    42001 ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat
    42061 tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg tgttgtacat
    42121 caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg gcacggctcc
    42181 ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat
    42241 cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata ttacctgtgg
    42301 ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga tcaccgtgcg
    42361 ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa tgaaagctat
    42421 ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc
    42481 accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc agcttggcgt
    42541 tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt accagccgcc
    42601 gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta aataattcgc
    42661 gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct
    42721 gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag tgcgcaagaa
    42781 ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc
    42841 gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc
    42901 gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc gcttggttgc
    42961 agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt aagcccggaa
    43021 gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga taacggtatt
    43081 tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat tgttaccgat
    43141 caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc taacttgcgc
    43201 gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta tctgatgctg
    43261 gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat cgcaattcct
    43321 aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt cgtgccgctg
    43381 gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc cggaaagtgc
    43441 cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc
    43501 agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg
    43561 tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat
    43621 gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc
    43681 ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg
    43741 tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt
    43801 tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt
    43861 cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac actctacccc
    43921 ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat
    43981 aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct ggccccttcg
    44041 ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat gaccatggaa
    44101 gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga gccgctgttt
    44161 gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg
    44221 gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt cgataccgct
    44281 ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga tatcaacgag
    44341 tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat ttagcgtcat
    44401 cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc gcattttgac
    44461 tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa tcgcattcag
    44521 gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg ccaagaaaga
    44581 gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg aacccgaacg
    44641 cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca
    44701 aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag aagctgacga
    44761 cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa aactggcgca
    44821 gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt acctcgatat
    44881 tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg agcgtatgta
    44941 tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt cggaaggtga
    45001 agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt acggctggct
    45061 gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg
    45121 tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg atattgatat
    45181 cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca
    45241 gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg ggatcgtgac
    45301 ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga agcagatgaa
    45361 agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga
    45421 taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg atgactcccg
    45481 cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc tggacggcag
    45541 cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca acatgtctga
    45601 acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga aaacgcgtcg
    45661 tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga
    45721 tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag aactggaagc
    45781 gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta tgggtaaagc
    45841 gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga
    45901 tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg acatcctcgg
    45961 cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact gtaccgagct
    46021 gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct tgcaggatca
    46081 ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat cccgtaacac
    46141 ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga accgcggctt
    46201 tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt
    46261 tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc cggaactgta
    46321 cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg
    46381 taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac tctacatggc
    46441 ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta ctctggcaca
    46501 ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact tcggtaaacc
    46561 gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa ccgacatggc
    46621 ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta tccacgttga
    46681 gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg cagaagcaca
    46741 tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc tggcgcgtcg
    46801 taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat
    46861 cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct tcctggatca
    46921 ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag attacgttac
    46981 cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg accgtatggt
    47041 aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc
    47101 ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa
    47161 gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc
    47221 acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct aaatcacacc
    47281 attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa
    47341 ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg tagcgaaagt
    47401 caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg caacggcgga
    47461 tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag ttagtccaca
    47521 cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc cccttggcat
    47581 taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt gcggcatact
    47641 tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt tccgtttgca
    47701 taatttctca catgtaattc tgatcactca tcattataga taattgaaga gtgaaatgat
    47761 taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac
    47821 catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg caacacagtc
    47881 agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag cgcgcattaa
    47941 aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga agccacatac
    48001 gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg tcagcatgat
    48061 gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg agcataatgc
    48121 cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg tgagagagcg
    48181 tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa cggttggtgg
    48241 tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg aacatcacta
    48301 gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc atttttggca
    48361 ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg ctatgaacgc
    48421 gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag ctgttaaacg
    48481 tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata tcgtgtgaag
    48541 agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac tcgataaaca
    48601 cgatgat
//
LOCUS       DAEEDS010000024        48313 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEEDS010000024 DAEEDS010000000
VERSION     DAEEDS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48313
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.024"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            187..480
                     /locus_tag="KLH32_001444"
     CDS             187..480
                     /locus_tag="KLH32_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE0138324.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KLH32_001445"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KLH32_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBE0138325.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="KLH32_001446"
     CDS             complement(1922..2374)
                     /locus_tag="KLH32_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBE0138326.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KLH32_001447"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KLH32_001447"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBE0138327.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="KLH32_001448"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="KLH32_001448"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBE0138328.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KLH32_001449"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KLH32_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBE0138329.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KLH32_001450"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KLH32_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBE0138330.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KLH32_001451"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KLH32_001451"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBE0138331.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KLH32_001452"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KLH32_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBE0138332.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KLH32_001453"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KLH32_001453"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE0138333.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KLH32_001454"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KLH32_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBE0138334.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KLH32_001455"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KLH32_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBE0138335.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="KLH32_001456"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="KLH32_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBE0138336.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="KLH32_001457"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="KLH32_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBE0138337.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="KLH32_001458"
     CDS             complement(15085..15582)
                     /locus_tag="KLH32_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBE0138338.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="KLH32_001459"
     CDS             complement(15579..15941)
                     /locus_tag="KLH32_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBE0138339.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="KLH32_001460"
     CDS             complement(15931..16278)
                     /locus_tag="KLH32_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBE0138340.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="KLH32_001461"
     CDS             16387..16800
                     /locus_tag="KLH32_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138341.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="KLH32_001462"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="KLH32_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="KLH32_001463"
     CDS             complement(18324..20039)
                     /locus_tag="KLH32_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBE0138342.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="KLH32_001464"
     CDS             20206..21099
                     /locus_tag="KLH32_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBE0138343.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="KLH32_001465"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="KLH32_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23527)
                     /locus_tag="KLH32_001466"
                     /pseudo
     CDS             complement(21910..23527)
                     /locus_tag="KLH32_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23822..24496
                     /locus_tag="KLH32_001467"
     CDS             23822..24496
                     /locus_tag="KLH32_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0138344.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="KLH32_001468"
     CDS             complement(24535..26196)
                     /locus_tag="KLH32_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBE0138345.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KLH32_001469"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KLH32_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBE0138346.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KLH32_001470"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KLH32_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBE0138347.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="KLH32_001471"
     CDS             27650..27982
                     /locus_tag="KLH32_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBE0138348.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29203)
                     /locus_tag="KLH32_001472"
                     /pseudo
     CDS             complement(27984..29203)
                     /locus_tag="KLH32_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            29304..30404
                     /gene="cbrA"
                     /locus_tag="KLH32_001473"
     CDS             29304..30404
                     /gene="cbrA"
                     /locus_tag="KLH32_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBE0138349.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30365..31702)
                     /locus_tag="KLH32_001474"
     CDS             complement(30365..31702)
                     /locus_tag="KLH32_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0138350.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31777..32925)
                     /gene="dgoD"
                     /locus_tag="KLH32_001475"
                     /pseudo
     CDS             complement(31777..32925)
                     /gene="dgoD"
                     /locus_tag="KLH32_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32922..33539)
                     /gene="dgoA"
                     /locus_tag="KLH32_001476"
     CDS             complement(32922..33539)
                     /gene="dgoA"
                     /locus_tag="KLH32_001476"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBE0138351.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33523..34401)
                     /gene="dgoK"
                     /locus_tag="KLH32_001477"
     CDS             complement(33523..34401)
                     /gene="dgoK"
                     /locus_tag="KLH32_001477"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBE0138352.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34398..35087)
                     /gene="dgoR"
                     /locus_tag="KLH32_001478"
     CDS             complement(34398..35087)
                     /gene="dgoR"
                     /locus_tag="KLH32_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBE0138353.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35365..36021
                     /locus_tag="KLH32_001479"
     CDS             35365..36021
                     /locus_tag="KLH32_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138354.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36067..36879)
                     /gene="yidA"
                     /locus_tag="KLH32_001480"
     CDS             complement(36067..36879)
                     /gene="yidA"
                     /locus_tag="KLH32_001480"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBE0138355.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36993..37391)
                     /locus_tag="KLH32_001481"
     CDS             complement(36993..37391)
                     /locus_tag="KLH32_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBE0138356.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37631..40045)
                     /gene="gyrB"
                     /locus_tag="KLH32_001482"
     CDS             complement(37631..40045)
                     /gene="gyrB"
                     /locus_tag="KLH32_001482"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBE0138357.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40074..41147)
                     /gene="recF"
                     /locus_tag="KLH32_001483"
     CDS             complement(40074..41147)
                     /gene="recF"
                     /locus_tag="KLH32_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBE0138358.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41147..42247)
                     /gene="dnaN"
                     /locus_tag="KLH32_001484"
     CDS             complement(41147..42247)
                     /gene="dnaN"
                     /locus_tag="KLH32_001484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBE0138359.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42252..43655)
                     /gene="dnaA"
                     /locus_tag="KLH32_001485"
     CDS             complement(42252..43655)
                     /gene="dnaA"
                     /locus_tag="KLH32_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBE0138360.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43952..44032
                     /locus_tag="KLH32_001486"
     CDS             43952..44032
                     /locus_tag="KLH32_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138361.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44262..44402
                     /gene="rpmH"
                     /locus_tag="KLH32_001487"
     CDS             44262..44402
                     /gene="rpmH"
                     /locus_tag="KLH32_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBE0138362.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44419..44778
                     /gene="rnpA"
                     /locus_tag="KLH32_001488"
     CDS             44419..44778
                     /gene="rnpA"
                     /locus_tag="KLH32_001488"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBE0138363.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44742..44999
                     /gene="yidD"
                     /locus_tag="KLH32_001489"
     CDS             44742..44999
                     /gene="yidD"
                     /locus_tag="KLH32_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBE0138364.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45002..46648
                     /gene="yidC"
                     /locus_tag="KLH32_001490"
     CDS             45002..46648
                     /gene="yidC"
                     /locus_tag="KLH32_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBE0138365.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46754..48118
                     /gene="mnmE"
                     /locus_tag="KLH32_001491"
     CDS             46754..48118
                     /gene="mnmE"
                     /locus_tag="KLH32_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBE0138366.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11992 a        13059 c        12206 g        11056 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat
    29161 tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg tcctgataca
    29221 aaacggttcc cgccatcata cttcacaagg acttcacttt cagacaatag tcttttgcca
    29281 tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt ggcctcggcc
    29341 cggcagggtc ggcgttggca cgaaagttag ccggcaaaat gcaggtgatc gcgctggata
    29401 aaaagcacca gtgcggtact gaaggtttca gcaaaccttg cggcggtctg ctggcaccgg
    29461 acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtg atcgccaatc
    29521 cacagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc
    29581 gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg
    29641 ccagcgttga ggtttatcac gatagcctgt gccgaaaaat ctggcgtgag gatgataaat
    29701 ggcatgtcat ttttcgtgca gacggctggg agcaacatat taccgcccgc tatctggtcg
    29761 gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat caaatccgta
    29821 aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcctgca
    29881 tctttgataa tgcgataact gactgttact catggagtat cagcaaagat ggttatttta
    29941 tctttggcgg tgcctatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga
    30001 aaatgagcgc ctttcagttc cagtttggca aagcggtgaa aagtgagaaa tgcacggtgt
    30061 tgtttccctc acgctggcag gattttgtct gtggtaagga caacgccttt ctgattggtg
    30121 aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg ctggatagcg
    30181 cagagattct gcgtgcggtg ttcctgaaac agccggagaa gagcaacgcc gcttactggc
    30241 gcgccacaca caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga
    30301 ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcgcatatt ccacagttga
    30361 aagattatcc aacgcgcttc acatcaccca ccagcaggat gtaagagagc gcgccaatca
    30421 acgcgacggc ggagatataa accagtgcag gcgcgaaacc gtaaccctgc gccaggtacc
    30481 ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac acgccgccgg
    30541 ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta atcgaagcaa
    30601 aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga tcgttggtgt
    30661 agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc gttttacgcg
    30721 caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca gagagcagga
    30781 cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc agcgccgtga
    30841 ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac agtgcagaag
    30901 ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc agtttccagt
    30961 ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca tccaccagac
    31021 cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc aggcgcggcg
    31081 gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca gtgacaatga
    31141 acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc aaaaacgcca
    31201 gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca tgttccggga
    31261 accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta
    31321 tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccttggaat aaagtcacca
    31381 ctgaccaacc aaatatcgca ataaaataag tcacgcgaga acctacgcga tctaaaaacc
    31441 aaccgccggg gatctggcat agcgtataaa gccaggcgaa ggccgaaaat acatagccca
    31501 tttccgcttt cgtaatgccg aactcttcct gaatatgggc ggaagccacg gccaagttag
    31561 cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc agataacgcc
    31621 gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc cagattttgg
    31681 gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt ttgtattgct
    31741 gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta cgctgttatc
    31801 ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact caataacttt
    31861 agcttcgtcg atttccacac ctaagcccgg tttcgttaac ggtttaaaga agccgccaac
    31921 catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg
    31981 aatgcccata ctttgttact gaagtaccgc gttataggaa acaaagtcga tatgcaggca
    32041 agccgccagt gcaatcggtc cgaacggaca gtgcggcgca agggtcacat cataggcttc
    32101 tgccattcca gcaattttgt agcattcggt aataccgcct gcatgggaga gatccggttg
    32161 cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg
    32221 ctcacccgcc gccagtggaa tatgcgtttg tgccgccaat ttcgggtagt attcggcttg
    32281 ttccgccagc accggctctt caataaacag cgggcgatac ggctccagtt ctttaatcag
    32341 cactttcgcc attggcgcgc tgacgcgacc gtggaaatca agaccaaact caatctgatt
    32401 gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg cgcgggagtt
    32461 atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg
    32521 cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc aactgtaggc
    32581 tttaattttg tcgcgaacca ggccgcccat cagttgccag actggcgcat tcagcacttt
    32641 gcctttgata tcccataacg cctggtcgat cccggcgatg gcgctcatca ggattggacc
    32701 gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc gcgaaggatc
    32761 ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac gggcgcggcc
    32821 ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt tcaggaacat
    32881 ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac tgcctctcga
    32941 tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg cccggcgcga
    33001 taaagatcgc tgcctaagcc cgccccaaca caacctgcgt ctatccactg cgccaggttt
    33061 tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac cgcttttaac
    33121 gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc
    33181 gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcaaac ggtcatgccg
    33241 tagcccaccg cgcggcggat cacttcacta tggatattgg gcgtaacgat gagctgacag
    33301 cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc gccaatcaac
    33361 gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg tggggaattc
    33421 agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc
    33481 tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg ccactgcatg
    33541 agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg ccacgtcgca
    33601 acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg cgacaagggt
    33661 gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc caatcaacaa
    33721 accagagaga aattcgctga cctgttcgcg gggaagtgtt cccagcacat gcgaggcgcg
    33781 aacttcaaaa agctgcggca atatggcggg cgcattaagg ccgcgctcaa ggccagccgc
    33841 gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg ccaatcaatg agtgatttaa
    33901 cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa tcgttgattt gctggctatc
    33961 ggcctgcacc catttgcaat gcgtaccggg cataacataa agagaggaag gagccagagc
    34021 tcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca ttgtggttat cgtcatgaga
    34081 gacgcataat ccgggaataa tccagatatt gtcgccaaca gacgttaatt gttcgccaat
    34141 agacgaaaaa cgggcaggaa cagataaata cggagcaact ttccagccga cattgctgcc
    34201 gaccattcct gccatcacca ctggcgtatt ttcttcacgc cagtcggtcg tgacttctgc
    34261 taacaccaca gccggagatt ttccgttcag gcgcgtgacg cctgcttctg attgcctgct
    34321 ctccaggcag tggtcgccct gataaagcca ggcgcgcaga ttggtcgatc cccagtcaat
    34381 tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct ggcaatcatg
    34441 gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc gtatcgcatc gaacagcgcc
    34501 ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt tcgttcaaaa
    34561 accgcccgct gcagcgaact gatcgcaatg ctaagttgct gtaacaccgg gttatgcacc
    34621 gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc gcggtcctga
    34681 ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct ggaggtcgcg
    34741 cgctctgctg cccaacgggc aatcgccggt tccaccagat ttcgcacttc gctcatggca
    34801 ctgataagcc gtgggtcgta gtcgttttcc agcacccatt gcagtacgtc agtgtcgagg
    34861 taattccact ggttacgcgg tgccacaaac gccccgcgat aacgtttcat ttcaatcagc
    34921 cgcttcgcca tcagcgaacg gaacacctca cggatgatgt tgcgcgaggt tgcaaactct
    34981 tcacagagct ccgcctcagc cggaagtggc gagccaggta cgtatttgcc gtgaacgatc
    35041 tgtttaccca gcgtaatgac aatgcgatcg gttttattga gagtcatgga gagtccttgt
    35101 gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa ctttgtagta
    35161 ccagcgtgat gacgttcgcg tttgccgcgc gcgtaatgta gtacaaactt atattgttat
    35221 gctacaattt agatcaaaaa aagaacaata cataaaaaaa tgacatgcgt cgggcagaaa
    35281 tctgaaaagg gatatcaggc gctaaacagg agggaaagaa gagtatgctt tcaacggctt
    35341 agctactcgt ttaaaggatt aatcatgaag ttgaatttta agggattttt taaggctgcc
    35401 ggtttattcc cactggcgct gatgctttca ggctgtatct cgtatgctct ggtttcccat
    35461 accgcaaagg gtagttcagg aaagtatcaa tcgcagtcag acaccatcac tgggctatcg
    35521 caggcaaaag atagtaatgg aacaaaaggc tatgtttttg taggggaatc gctggattac
    35581 cttatcactg atggtgccga tgacatcgtt aagatgctca atgatccagc acttaaccgg
    35641 cacaatattc aggttgccga tgacgcaaga tttgttttaa atgcggggaa aaagaaattt
    35701 accggcacaa tatcgcttta ctactactgg aataacgaag aagaaaaggc actggcaacg
    35761 cattatggtt ttgcctgtgg tgttcaacac tgtaccaggt cactggaaaa cctaaaaggc
    35821 acaatccatg agaaaaataa aaacatggat tactcaaagg tgatggcgtt ctaccatcca
    35881 tttaaagtgc gattttatga atactattca cccagaggca ttccggatgg tgtttccgca
    35941 gcattactgc cagtgactgt tacactggac atcattactg caccgctgca atttctggtt
    36001 gtatatgcag taaaccaata atcagtaagc gggcaaacgc gtttatgctg tttgcccgct
    36061 tactgattaa ttcagcacat acttctcaat agcaaacgcc acgccatctt caaggttgga
    36121 ttttgtgaca aagttcgcca cttctttcac cgaaggaata gcgttatcca tcgccacacc
    36181 gacgcctgca tattcaatca ttgcgatgtc gttttcctga tcgccaatcg ccatgatttc
    36241 ttccggttta atacctaaca cgtcggccag tgatttcaca cccgtacctt tgttaacgcg
    36301 tttatcgagg atttcgagga agtacggcgc acttttcagc acggtatatt tctctttcac
    36361 ttcctgcgga atacgcgcga tagcctggtc gaggatggcg ggttcatcaa tcatcatcac
    36421 tttcaggaac tgggtattgg ggtccatttt ctccgcttcg cagaacacca gcggaattgt
    36481 ggcaacgaag gattcatgca ccgtgtagta gctgatatcg cggttagcgg tgtacagcgt
    36541 ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga gagagttttt ccaggaaacg
    36601 atagtcgtca tagctgagag cggtttgcgc cacggtgcta ccatcagcgg ccttctgtac
    36661 cagcgcgccg ttataagtaa tgcagtagtc gcccggctgt tccatatgca gctctttcag
    36721 gtagttgtgc acacctgcat acggacgacc cgtcgttagc acgacattca cgccacgggc
    36781 gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg gtgtgatcgg gcagcagaag
    36841 ggtgccatcc atatcgatag caatgagttt gatagccatg agttcccaag gtagattagt
    36901 tcctgaccca tgctaacgcg attccgctca aaaatcagta caacacccga gggaaaaggg
    36961 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    37021 tccatgcctg cggaaagcag atcgttattg gcttgtgctg acacttcacc ttgcggtgag
    37081 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagccgt actggtatcc
    37141 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ctccagttgc
    37201 tcgccgctaa ctgattgatt tctctgttga ttactcaacc aggttgagag aatggcccct
    37261 aagccgccac tttgcagttt ttccagcagc acctgaatgc caccctgctc ctcaacccaa
    37321 cttaaaatcg cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    37381 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatctg
    37441 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    37501 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgcaaaat
    37561 caatatattg caggagtctc gtaggactaa taagcgtagc gcatcaggca cgctcgcatg
    37621 gttaacgcca ttaaatatca atattcgccg ctttcagggc gttctcttca ataaacgcac
    37681 gacgcggttc tacggcgtcg cccatcagcg tggtgaacaa ctggtcagcg gcaatcgcat
    37741 ccttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    37801 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggcca cgacgggact
    37861 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    37921 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    37981 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    38041 gggtacgcac gcgaacaatc ggctcgaaca ggttttgttc ggcattggtg tggacatcaa
    38101 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    38161 agcgggtaac ggtctgctca tcagaaaggt cagcttccgt caacgtcggc tgatagataa
    38221 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    38281 cgttgtactc agataccagt ttctctaacg cttcgccagc caatgccggt gcactggcgt
    38341 tggtgtgcag cgttgcgccg tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    38401 cgtctttaat gtactgttcc tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    38461 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaagaag gtcaacagca
    38521 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    38581 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    38641 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcgacg ttaaggattt
    38701 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    38761 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    38821 ctgccagttt gcccggcagg ccagctaagt cgagcgcacc tttacggcgg gtcatttcac
    38881 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    38941 tcgcatcagt tgggttttcc agcaggtatt ccgccagcag ttcgttcatc tgctgttcaa
    39001 ccgccgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    39061 ccggcacttt tacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    39121 tgactttggc ttttttgctg tagccttctt tgtccatgta ggcattcagg gtacgggtca
    39181 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    39241 agcagtagat gttttcctgg aagccatcgt tccactgcag cgccacttca acgccaatac
    39301 cgtctttttc ggtggagaag tagaagatat ttgggtggat cggcgttttg ttcttgttca
    39361 gatattcaac gaacgctttg atgccgcctt catagtggaa gtggtcttct ttgccgtcac
    39421 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    39481 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    39541 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccagcgga gcctgcggta
    39601 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctgaataacc agctccagtt
    39661 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    39721 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    39781 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    39841 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    39901 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    39961 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    40021 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatct
    40081 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    40141 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    40201 agcaacccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    40261 cgccgcccgc tttcacgagt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    40321 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    40381 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    40441 cgttccagca cctcagcgta ctctgtctct ttctcccagc cgcgctggaa agagaaagtc
    40501 agagaaaact cagggagaaa ttgcttacag gtatcatcca tatcggccgc gataccggcg
    40561 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagttc tttatcccac
    40621 gggcgtagct gttcgtaacg agtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    40681 cgcttgagat tgctccaggc ggtgaaaaat ccaggttcgt tgtgaaagca tccccagtcg
    40741 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    40801 aactgcatcg gcatcaggtg cgccagttcc gcgactttat gcccgtccgt accgtcaata
    40861 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgccgtctc gcgctcttcg
    40921 ccctgtaatc gcccgtggag aacaaatgcc tcctgctcat ggcgaatgac gcgaccaatc
    40981 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    41041 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    41101 gtttcaatat tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    41161 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    41221 aatcggtcag catcatgcgg acgttttcgc atttcagcgc gttcagaaca tccagcacat
    41281 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    41341 ccgcttcttc ctgttccggg ttgttggcag taattttcag ctggttttcg ctgacataca
    41401 ggcgcacacc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    41461 gcagatcgca gcctgcttcc agatgtttgt ccgggttctt cggcagaacg cgacggtaat
    41521 ccgggaagcg accatccacc agtttcgagg tgaaaataaa gtcgccaacg tgggcacgaa
    41581 tgttattgct gccaatctgc acacgcagca gattgtcgcc gccgtcgagc atacgcatca
    41641 gttcaatcac gcctttacgc ggtacgatca ccgaatggct tggcaaagat tgaccaattg
    41701 gcattgaaca gaccgccaga cggtggccgt cggttgccac ggtgcgcagt tcttcacctt
    41761 cggtttcaaa cagcataccg tttaagtaat agcgaacatc ctgatgcgcc atcgaaaact
    41821 gggttgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    41881 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    41941 gcccggagcg caccagcatc cgttcacctt ccagctgcac ggcaatttcc gcgccttcag
    42001 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    42061 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    42121 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    42181 cacctaacgg accgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    42241 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    42301 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    42361 cgcccaccaa acgcatcgcc aatctctggc agactgtggt tagtcagctc tttcgctagc
    42421 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    42481 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    42541 ttttcctgca atgccagcaa gtcgcgcagc gcctcacgca cgaagtcgat ggtgatcgcc
    42601 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    42661 ttagatcgta gacgcttggc gataaagaac gccacttcac ccggcaaacg aatgtcattt
    42721 tcgtcggcct ttttcatcag gatcgccaca cgggtttcca gctctggcgg ttcgatcgcc
    42781 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    42841 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    42901 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    42961 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    43021 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    43081 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    43141 ccataaagga acaacgggtt ataggcaccg ccagggttat ccgccacctg gcaagccgcc
    43201 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    43261 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    43321 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    43381 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    43441 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    43501 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    43561 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    43621 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    43681 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    43741 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    43801 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    43861 ctatttatcc acaggacttt ccagaactcg ctaagtgtaa acgatcctgc cgcgaggcgg
    43921 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    43981 atctaataaa gatccaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    44041 ccggcgcgcc atgctggttt ccactggtgt gaggtcgtac attttccctg cgaaaaggtg
    44101 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg tgtgtacaat tattacaatc
    44161 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    44221 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    44281 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    44341 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    44401 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    44461 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    44521 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    44581 aacgttcgac gcgcccatga acgcaatcgg attaaacgcc tgacgcgtga aagcttccgt
    44641 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    44701 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    44761 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    44821 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    44881 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    44941 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    45001 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    45061 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    45121 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    45181 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    45241 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgtt
    45301 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    45361 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    45421 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    45481 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    45541 gcagaacgct ggcgagaaac cgctggaaat ctcctcgttt ggtcagttga agcaatccat
    45601 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgtgg
    45661 cgcggcgtac tccacgcctg acgagaagta tgagaaatac aagttcgata ccattgccga
    45721 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcgatgctgc aacagtattt
    45781 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    45841 taacggcatc gtcgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    45901 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    45961 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    46021 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    46081 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    46141 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    46201 caaacagcgt atcagtcagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    46261 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    46321 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    46381 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    46441 gatgtcgccg accactgtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    46501 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtactgtact atatcgtcag
    46561 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    46621 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    46681 agttgaccgc cttttttctt ttcgtagggc ggataagcac cgcgcatccg ccacacaaag
    46741 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    46801 tggcggcgtt ggcatcctgc gcatctccgg cttcaaagcc cgtgaagttg ccgaaaccgt
    46861 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    46921 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    46981 agatgtgctg gaactgcaag gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    47041 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    47101 tcttaacgat aaacttgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    47161 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    47221 taatcatctg gtagaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    47281 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    47341 gatggccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    47401 gatgaaagta gtgattgccg gacgtcctaa tgccggtaaa tcgagcctgt taaacgcgct
    47461 ggcgggccgt gaagcggcaa tcgtaaccga tatcgccgga accacgcgtg acgtgctgcg
    47521 tgagcatatc cacattgacg gaatgccgct gcatatcatc gacactgccg ggctacgtga
    47581 ggccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    47641 cgaccgcgtg ctgtttatgg tcgatggcac cacgacagac gccgttgatc cggcagagat
    47701 ctggccggaa tttatcgccc gtctgcccgc gaaactgccg atcaccgtgg tacgcaataa
    47761 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    47821 tctctcggca aggactggtg aaggcgtgga cgtgctgcgt aaccatctca aacagagcat
    47881 gggctttgac accaatatgg aaggcggctt cctggcgcgc cgtcgccact tacaggcgct
    47941 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttgggag cctgggcagg
    48001 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    48061 atttacttca gacgacctgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    48121 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    48181 gttatgcctc aacccttttt taaacattaa aattcttacg taatttataa tctttaaaaa
    48241 aagcatttaa tactgctccc cgaacgattg tgattcgatt cacatttaaa caatttcaga
    48301 atagacaaaa att
//
LOCUS       DAEEDS010000025        47955 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEEDS010000025 DAEEDS010000000
VERSION     DAEEDS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.025"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="KLH32_001492"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="KLH32_001492"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBE0138367.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="KLH32_001493"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="KLH32_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBE0138368.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KLH32_001494"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KLH32_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBE0138369.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KLH32_001495"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KLH32_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBE0138370.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KLH32_001496"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KLH32_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBE0138371.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="KLH32_001497"
     CDS             5377..5835
                     /locus_tag="KLH32_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBE0138372.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="KLH32_001498"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="KLH32_001498"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBE0138373.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="KLH32_001499"
     tRNA            6887..6971
                     /locus_tag="KLH32_001499"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="KLH32_001500"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="KLH32_001500"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KLH32_001501"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KLH32_001501"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBE0138374.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KLH32_001502"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KLH32_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBE0138375.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KLH32_001503"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KLH32_001503"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBE0138376.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="KLH32_001504"
     CDS             complement(10252..13995)
                     /locus_tag="KLH32_001504"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBE0138377.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KLH32_001505"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KLH32_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBE0138378.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="KLH32_001506"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="KLH32_001506"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBE0138379.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="KLH32_001507"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="KLH32_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBE0138380.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KLH32_001508"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KLH32_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBE0138381.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="KLH32_001509"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="KLH32_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBE0138382.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KLH32_001510"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KLH32_001510"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBE0138383.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KLH32_001511"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KLH32_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBE0138384.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="KLH32_001512"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="KLH32_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBE0138385.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="KLH32_001513"
     CDS             23392..23499
                     /locus_tag="KLH32_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0138386.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KLH32_001514"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KLH32_001514"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBE0138387.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="KLH32_001515"
     CDS             complement(24538..25347)
                     /locus_tag="KLH32_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBE0138388.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="KLH32_001516"
     CDS             complement(25351..25743)
                     /locus_tag="KLH32_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBE0138389.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KLH32_001517"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KLH32_001517"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE0138390.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KLH32_001518"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KLH32_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBE0138391.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KLH32_001519"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KLH32_001519"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBE0138392.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="KLH32_001520"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="KLH32_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBE0138393.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="KLH32_001521"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="KLH32_001521"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBE0138394.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="KLH32_001522"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="KLH32_001522"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBE0138395.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="KLH32_001523"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="KLH32_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBE0138396.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KLH32_001524"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KLH32_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBE0138397.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="KLH32_001525"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="KLH32_001525"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE0138398.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="KLH32_001526"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="KLH32_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBE0138399.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36789..39656
                     /locus_tag="KLH32_001527"
     CDS             36789..39656
                     /locus_tag="KLH32_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0138400.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSPDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="KLH32_001528"
     CDS             complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="KLH32_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBE0138401.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41903..42973
                     /gene="dhaK"
                     /locus_tag="KLH32_001529"
     CDS             41903..42973
                     /gene="dhaK"
                     /locus_tag="KLH32_001529"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBE0138402.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42984..43616
                     /gene="dhaL"
                     /locus_tag="KLH32_001530"
     CDS             42984..43616
                     /gene="dhaL"
                     /locus_tag="KLH32_001530"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBE0138403.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43627..45045
                     /gene="dhaM"
                     /locus_tag="KLH32_001531"
     CDS             43627..45045
                     /gene="dhaM"
                     /locus_tag="KLH32_001531"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBE0138404.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45365..47062
                     /gene="treA"
                     /locus_tag="KLH32_001532"
     CDS             45365..47062
                     /gene="treA"
                     /locus_tag="KLH32_001532"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBE0138405.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47141..47580)
                     /locus_tag="KLH32_001533"
                     /pseudo
     CDS             complement(47141..47580)
                     /locus_tag="KLH32_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12036 a        12343 c        11747 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcta
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccac ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aagcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaagc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ttggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta cgacaactta gggaataact cgatataaac tcacctttgt
    36601 aagggggggg ttaaatatat attaattcct gtgttcctga ttttgcccgg taatagagaa
    36661 ttattctcag agaagctcat catgattgca ccatacagat atcatgatga taagaggata
    36721 ttattcattg acttaaacaa aatgacaaaa tccatagacc catagcctgg gtactatata
    36781 ggagtggtat gggcatcaaa caacacaatg ggaataccaa agccgatcgt ctcgctgaat
    36841 taaaaatccg ttcgccctca attcaactga taaaatttgg cgctattggt ttgaatgcaa
    36901 ttatcttttc ccccctgctg atagctgctg atacaggaag tcaatatggc accaatatta
    36961 ctattaatga tggtgacaga attacaggag ataccgccga tccatcagga aacctctatg
    37021 gtgtaatgac cccagcagga aacacgcctg gcaatatcaa cctgggtaat gatgtcaccg
    37081 tcaatgtcaa cgacgcctct ggatatgcaa aaggaatcat tattcagggc aaaaacagct
    37141 ccctgacagc taaccgactc acagtagatg ttgttggtca aacctctgcc atcggcatta
    37201 acttaattgg tgactatacc catgctgact taggcacagg cagcaccatt aagagtaacg
    37261 atgacggcat cattattggg catagctcaa cactaacagc cactcaattc accattgaaa
    37321 actcgaacgg tataggccta accatcaatg actatggcac cagtgtcgat cttggaagcg
    37381 gaagtaaaat caagaccgat ggaagtacag gtgtttatat cggtggtctc aacggcaata
    37441 acgccaatgg tgctgcgcgt tttacggcga cagacctgac aatcgatgtt cagggctaca
    37501 gcgccatggg gataaacgta cagaaaaact ctgttgtcga tctcggaaca aacagtacca
    37561 ttaaaaccaa tggcgataat gctcacggcc tctggagctt tggccaggtt agcgcgaatg
    37621 cactcactgt tgatgtaact ggagccgcgg ccaatggcgt cgaagttcgt ggtggtacaa
    37681 ccactatcgg tgcagatagc catatttctt ccgcgcaggg cggtggcctc gtcaccagta
    37741 gtccagacgc gacaatcaat ttttctggca cggcagcgca acgaaacagc atcttttccg
    37801 gcggttctta tggtgcctcg gcccagacgg caacggctgt tatcaacatg caaaataccg
    37861 atattacggt tgatcgtaat ggcagtctgg cgctgggttt gtgggcgctc agcggcggta
    37921 gaataaccgg agacagtttg gctatcaccg gcgcggcagg agccagaggg atttatgcca
    37981 tgaccaacag ccagatcgac ctcacgagcg atctggtcat tgatatgagt acacccgacc
    38041 agatggccat cgcaacgcaa catgacgatg gttatgccgc cagccgcatc aacgcctcgg
    38101 gtcgtatgct tatcaacggt agcgttcttt ccaaaggtgg gctaatcaat ctggatatgc
    38161 accctgggtc ggtttggaca ggttcctccc tcagcgataa tgtcaatggc gggaaactgg
    38221 acgttgcaat gaataacagc gtctggaacg taacaagtaa ttctaatctc gacacgctgg
    38281 cgctgagcca ttcaactgtc gattttgcca gccacgggtc aactgccggc acatttacca
    38341 cattaaacgt agagaacctg agcggtaaca gtacctttat tatgcgtgct gatgttgttg
    38401 gcgagggtaa tggcgttaat aatagagggg atttattgaa tatcagcggg agtagtgctg
    38461 gtaatcacgt attggctatc cgcaaccagg gcagcgaggc cacaacggga aatgaagttc
    38521 tgacagtggt aaaaaccact gacggcgcgg cctcgttcag cgcgtcttct caggttgagt
    38581 tggggggata tctgtacgat gtgcgtaaaa atggcactaa ctgggagctt tacgcttccg
    38641 ggacagttcc ggaaccgact cctaatcctg aacccacacc agctcccgct cagcctccca
    38701 tagtcaaccc cgatcctacg cctgaacccg ctcccacgcc taaacccacc acgaccgcag
    38761 atgctggcgg caattatctc aatgtcggtt acttattgaa ctatgttgaa aaccgtacgc
    38821 tgatgcaacg gatgggtgac ttgcgaaatc agagtaaaga cggtaatatc tggttgcgca
    38881 gttatggggg aagcctggac tcctttgcca gtggcaaact gagcggcttt gacatgggtt
    38941 acagcggtat ccagtttggt ggggataaac gtctctctga tgtaatgccg ttgtatgtcg
    39001 gtctgtatat tgactcaaca catgcatcgc cggactatag cggaggcgac ggtaccgcac
    39061 gttcagacta catgggaatg tacgccagtt acatggcaca aaacggtttt tacagcgatc
    39121 tcgttataaa agcatcgcgc cagaaaaata gtttccacgt actggacagt cagaacaacg
    39181 gcgttaacgc caacggcact gcgaatggaa tgagcatctc cctggaagcc gggcagaggt
    39241 tcaacctgtc ccctactggt tatgggttct atatagagcc gcaaacccag cttacataca
    39301 gccaccagaa tgagatggct atgaaggcga gtaatggcct caatatacat ctgaatcact
    39361 acgaatcgct gctggggcgt gccagcatga tactggggta tgacatcacc gcaggcaaca
    39421 gccagctgaa tgtctatgtg aagactggcg ctatccgcga gttttcaggg gataccgaat
    39481 atctgttgaa cgactcccgg gagaagtaca gtttcaaagg taatggctgg aataacggcg
    39541 tgggagtcag tgcacagtat aacaaacagc acacattcta tctcgaagcg gattacacgc
    39601 agggtaacct ctttgatcag aagcaagtca acggaggata tcgcttcagc ttttaagtgc
    39661 gggatagaca agcccttaaa cgatgctgaa aattgttagc gtgcgccgga atgaaataca
    39721 gatcactctg atgtatcaga aatcgtttct gtctttcata cccggcgctt aaactgccct
    39781 gcatcaatgc catgttgctt cattttccgc cacagcgtag tgcggccgat cccaagtaaa
    39841 gccgacattt cctgaatgcg accgcctgtg acctgggctg cgttaataat tgcctctttt
    39901 tcaacttccg caaatgacag actggtggaa aggcgggtgg cgctgacatc atctgtcgcc
    39961 tgctcggtaa acagatgttc aggtaaatca ctgacgcgaa tgcgcccgtt atcactactc
    40021 agagcaagat tctcgatgac gctgtaaagt tcaaaatcgt tgcctggcca tgcacaagaa
    40081 actaggcgag cgagggcatc gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga
    40141 ctgcgtaatt tgttattcac cagcgccgga atgctgccac gccgcatacg cagaggcggg
    40201 atggtaattt caaatgcatg cagcgcgtaa tacagctggc gactaaaacg attttgttcc
    40261 accagcattg cgaggtccac ggtcgttgtt gcaatcactt tgacatcaat tggtattaaa
    40321 cgccgcgcat ccagtcgcgt gataaccccc tgcttgataa cctgcagcaa agcagactgt
    40381 aactccaccg ccagatattc aatcttttca agaaacagcg tgccgccgtg tgccagttcc
    40441 agccgactca gacggccatt ttcattgtcc gtgcgatcgc caccaataaa ttcttccgcc
    40501 agcgcagcat caccatataa ctcacaattg acggcgatat aaggacctgc agcacgctcg
    40561 ctttcattat gaattgcctg acttagcagt gccttgccca cgccctcttc tccacaaagc
    40621 aggacaggaa agctactgcg cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc
    40681 ggatcgtcct gtggcatatg agcgaaggta tggctgactt ttcctaattg actggtcatt
    40741 aactgccgca tctgttccac aggatggagc aacaaaataa agctggttcc ctgcgtttct
    40801 attatcggtt taagggttat caccgcatca ataaactggt gctggctttc aaaggttgct
    40861 tctacgtgtt tgagcggatg tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt
    40921 aagagttcag tgattgcccg tccctgactt gccgtcgcgt caaggcgcaa gacccgcgcc
    40981 gcctgggcat taataaattg caaattaccc tgctcgtccc agctaatcac gccatcatcc
    41041 atactttcta acagggcatt aagttgattt aaatgacggt tagtttcagc gagcaaactg
    41101 tccgtcagca gtaaattacc gacctcgcgg gcgattgcca acgtcaacgg caaatcagct
    41161 gcggtagttt gctcaaccgg acacgccagc gctattgttc ccgtcaatcg gcccttgctg
    41221 tcaaacaacg gcgttgcaca aaaggcccag ttccagagtg cctgtttgaa atgttgatcg
    41281 gccatcgttt tcacggcctg accagagata gccgctaacg atagcgcaca agtaccaata
    41341 attccctcgg cgcaatacgt gccgtcattg aatcccagtg cacttagctg ctgcaaggtt
    41401 tgcggatcgc cattacggct gagaatgcag gcggtttcat cgaggataaa cagcgcacac
    41461 tctcgcggtg ccatatattc ccaggcatct tccagcgcag cctgcccgag cgtcagcatc
    41521 gctttcttac gccgataaat agaagcaaat gtcacgccct gggcctgatg tggtacgttc
    41581 catgtctccc gtttcatcag cttattgcat cgctcccagg aggttgcaat taagggagat
    41641 atgccccgac catcgttgtt aaaagcgcca ctcatatccc gcatccctta tgacaaaagg
    41701 tgttccggct cgcaatttta agtattaata cacgtaaaag tttcaatatg gaacattcat
    41761 taaagcatat gtttcactat gaaacataat ttgggctaat tttcttttcc cttgcctgat
    41821 gcacaatgga ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta
    41881 ccgtaattgc tggagcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga
    41941 cgaacaactg gcaggactgg cgaaagcgca tccatcgctg acactgcatc aggatccggt
    42001 gtatgtcacc cgagctgatg cccctgttgc aggaaaagtc gccctgctgt cgggtggcgg
    42061 cagcggacac gagccgatgc actgtggtta tatcggtcag gggatgcttt cgggggcctg
    42121 tccgggcgaa attttcacct caccgacgcc cgataaaatc tttgaatgct ccatgcaaat
    42181 tgatggcggc gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt
    42241 tgaaacagcg accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga
    42301 tgacgacgtt gcggtaaaag acagtcttta taccgccggg cgacgcggcg ttgccaacac
    42361 agtattaatt gaaaaactcg taggcgcagc ggcggagcgt ggcgactcac tggaggcctg
    42421 tgcggaactg gggcgtaagt tgaataatca aggccactca ataggtatcg ctctcggtgc
    42481 ctgtaccgtt cctgccgcgg gcaaaccttc ttttaccctt gcggataatg agatggagtt
    42541 tggcgtcggc attcatggtg agccaggtat tgaccgccgc cctttctctt cccttgatca
    42601 aaccgtcgat gaaatgttcg acaccctgct ggaacatggc tcataccatc gcactttgcg
    42661 tttctgggat tatcaacaag gcagctggca ggaagaacca caaaccaaac aaccgctcca
    42721 gtctggcgat cgggtgattg cactggttaa caatcttggc gcaactccgc tttctgagct
    42781 gtacggcgtc tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgaacg
    42841 taatttaatt ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact
    42901 gaaagttgat gacgaaacgc tggctctctg ggacgccccg gtccacaccc cggcccttaa
    42961 ctggggtaaa taaggagaaa gcaatgtcac tgagcagaac tcaaattgtt aactggctca
    43021 ctcgttgtgg cgatattttc aacaccgaga gcgagtatct taccgggctg gatcgcgaga
    43081 ttggcgacgc tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac
    43141 tccctgctat cgcagataaa gatatcggtt tcattctcaa aaataccggt atgacgctgc
    43201 tttccagcgt cggtggtgcc agtggtccgc tgttcggtac cttctttatc cgcgccgcac
    43261 aggcgaccca ggcacggcaa agcctgacac tggaagagct ttatcagatg ttccgcgatg
    43321 gcgcggacgg cgtaatcagt cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg
    43381 tgtgggtgcc ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctgttccgg
    43441 tggcgctcga agctgccagt agcatcgccg aatccgctgc acaaagtacg attacgatgc
    43501 aagcccgcaa aggccgcgcc agttatctcg gtgaacgcag tattggtcac caggatcccg
    43561 gcgcgacctc ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa
    43621 ttggtgatgg taaacctggt catagtttca catagcagcc gactgggaga aggtgtcggt
    43681 gaattagccc gtcagatgtt aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt
    43741 gacgatccac aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct
    43801 gttgctgatg ccgaccatgt gctggtcatg atggatatgg gtagcgcatt attgagtgct
    43861 gaaactgcgc tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgcg
    43921 ccgttggtcg aaggtacact ggcagcaacg gtcagcgcgg cctcgggggc ggatatcgac
    43981 aaagttatct ttgacgctat gcatgcgctg gaagccaaac gtgaacaact gggtttaccg
    44041 tcctccgaca ctgaaatctc tgacacatgt cctccgtacg atgaagaagc ccgttctctg
    44101 gcggtggtca taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat
    44161 accttatcga catttaatgc cgatatgttg ctggaaaaaa acggcaaatg cgtcacacca
    44221 gagagtatta accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt
    44281 gcgaaagggc cagaagctga agaggcactg atcgctttcc gtcacctggc tgaagataac
    44341 tttggtgaaa cggaggaagt cgctccacct actctgcgtc ccgttctgcc tgtttcgggt
    44401 aaagcctttt attatcaacc agttttatgt acggtacagg caaaatcaac cctgaccgcg
    44461 gaagaagaac aagatcgatt acgccaggct attgacttca cgttattaga tctgatgacg
    44521 ttaacagcga aagcagaagc cagcgggctt gacgatattg ccgcaatctt ttctggtcac
    44581 catacactgt taggtgatcc ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa
    44641 cattgcacgg cagaatatgc ctggcagcaa gttcttaaag aacttagcca gcaataccag
    44701 caactggatg atgaatatct acaagctcgc tatattgatg tggacgatct tctgcatcgc
    44761 accctggtcc acctgaccca aacgaaagaa gaactcccgc agtttaactc gccaactatt
    44821 ctactggcgg agaacattta tccttccaca gtactgcaac tggatccggc ggttgtaaaa
    44881 ggtatctgcc ttagcgccgg aagtccggta tcccacagcg ccctaatcgc ccgtgaactg
    44941 gggattggct ggatttgcca gcagggtgag aaactgtatg cgatacaacc agaagaaacg
    45001 ctaacgctgg acgttaaaac gcaacgtttc aaccgtcagg gttaagccac cgttcggatg
    45061 gcatcgttct gatgtcatcc ggcaatttac ccagcactga tgttttttaa ctacctgtta
    45121 tattttttac caatttatga tgatttttct tcgctaatcg cgaaccaaat gtcgctggct
    45181 ggctaataaa cagacgttat ttgatcactc tatttcgccg ttatttatcc ctttaattcc
    45241 cttttctaaa atgcctgacc gttcgcagaa tgagatttcg atcatgcagc tagtgcgatc
    45301 ctgaactaag gttttctgat acttgaatac cgttttattc ggtttcgcca aaggagaatg
    45361 attgatgaaa tcccccgcac cttctcgccc gcaaaaaatg gcgttaattc cagcctgtat
    45421 ctttttgtgt ttcgctgcgc tatcggtgca ggcagaagaa acaccggtaa caccacagcc
    45481 gcctgatatt ttattagggc cgctgtttaa tgatgtgcaa aacgccaaac tttttccgga
    45541 ccaaaaaacc tttgccgatg ccgtgccgaa cagcgatccg ctgatgatcc ttgctgatta
    45601 tcggatgcag caaaaccaga gcggatttga tctgcgccat ttcgttaacg tcaatttcac
    45661 cctgccgaaa gaaggcgaga aatatgttcc gccagagggg cagtcactgc gcgaacatat
    45721 tgacggactt tggccggtat taacgcgttc taccgaaaac accgaaaaat gggattctct
    45781 gttaccgctg ccggaacctt atgtcgtgcc gggcggacgt tttcgcgagg tatattactg
    45841 ggacagttac ttcaccatgt taggacttgc cgaaagtggt cactgggata aagtcgcgga
    45901 tatggtggcc aattttgctc atgaaataaa cacttacggt catattccca acggcaaccg
    45961 cagttactat ttaagccgct cgcaaccgcc cttctttgcc ctgatggtag agttactggc
    46021 gcagcatgaa ggcgatgccg cgttgaagca atacctgccg caaatgcaaa aagaatatgc
    46081 ttactggatg gacggtgttg aaaacctgca agccggacaa caggaaaaac gcgttgtaaa
    46141 acttcaggat ggtacccttc tcaaccgcta ctgggacgat cgcgatacgc cacgaccaga
    46201 gtcatgggtg gaagatattg ccaccgccaa aagcaatccg aatcgacctg ccactgaaat
    46261 ttaccgcgac ctgcgctctg ccgctgcgtc tggctgggat ttcagctcgc gctggatgga
    46321 caacccgcag cagttaaata ccttacgcac caccagcatc gtaccggtcg atctgaacag
    46381 cctgatgttt aaaatggaaa aaatcctcgc ccgcgccagc aaagctgcag gagataacgc
    46441 gatggcaaac cagtacgaaa cgctggcgaa tgcccgtcaa aaagggatcg aaaaatacct
    46501 gtggaacgat caacaaggct ggtatgccga ttacgacctg aaaagtcata aagtgcgcaa
    46561 tcagttaacc gcggccgccc tgttcccgct gtacgtcaat gcggcagcga aagatcgcgc
    46621 caacaaaatg gcgacggcga cgaaaacaca tctgctgcaa cccggcggcc tgaacaccac
    46681 gtcggtgaaa agtgggcaac aatgggatgc gccaaatggc tgggcaccgt tacagtgggt
    46741 cgcgacagaa ggattacaaa actacgggca aaaagaggtg gcgatggaca ttagctggca
    46801 cttcctgacc aatgttcagc acacctatga ccgggagaaa aagctggtgg aaaaatatga
    46861 tgtcagcacc accggaacgg ggggcggcgg tggcgaatat ccattacagg atggctttgg
    46921 ctggaccaat ggcgtgacgc tgaaaatgct ggatttgatc tgcccgaaag agcaaccgtg
    46981 tgacaatgtt ccggcgacgc atccgaccgt taagtcagca acgacgcaac cctcaaccaa
    47041 agaggcacaa cccacacctt aaccagcgct tactccgaca acgtcattcg gcttctccgg
    47101 atggcgttgt cggcagataa caccatatct atggtttttt ctacacagac ggtttttcaa
    47161 gtcctggcgg gaagtattgc gcaaatgttt gccatacgtg gttgccgaag atagcgtcat
    47221 ttctacgggt aagtgtttca ctttcatcac ccggcgattt acgccgacgt tggctatata
    47281 aagcatcaat tgctaccgaa tattgttacg gtgttcactc atcaacgttt ttttgctttc
    47341 tggagttaaa ttaataatca cataatccag acactccaga gaatttagca accaaaaaaa
    47401 gaattcagac cattcctctt cgcgtaactg aagcattgcc gaacgggtat tttggcttaa
    47461 tctgccagaa ggattgatca attctaaatg ttcaataagc gcagccaaaa ccgcctcact
    47521 atccgcccca tttttaacgc ctgactggct actgtctaaa atatttattt tctgaatcac
    47581 accaaaaaca acgaaacaat aattaaaaaa ggattctgtt gttgttaccg ggaaccgact
    47641 tatttcgctc agaaagtcgt tcaaccagct ccgcagtgat aagattcgct gatgatacag
    47701 tgtatcctca ctgcgtgctg gaggtgccag tataatatgt tgatcaatac agtatttatt
    47761 gtgttcagtc gtttcctgat gcgtttggtt ctctgcggat tgtcgctggc gaaataaatg
    47821 gcttgcggta aatgccgtta cagagcagcg caacgcgtca attccttcgg agtgcccggc
    47881 ttttggcgag agttccggtt ttatgcgtcc aggtagcgga aatttcaccc gaatgagaaa
    47941 tgcattaata aaatg
//
LOCUS       DAEEDS010000026        47304 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEEDS010000026 DAEEDS010000000
VERSION     DAEEDS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47304
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.026"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="KLH32_001534"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="KLH32_001534"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBE0138406.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KLH32_001535"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KLH32_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBE0138407.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KLH32_001536"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KLH32_001536"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBE0138408.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KLH32_001537"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KLH32_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBE0138409.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KLH32_001538"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KLH32_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBE0138410.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KLH32_001539"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KLH32_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBE0138411.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KLH32_001540"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KLH32_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBE0138412.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KLH32_001541"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KLH32_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBE0138413.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KLH32_001542"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KLH32_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBE0138414.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KLH32_001543"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KLH32_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBE0138415.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KLH32_001544"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KLH32_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBE0138416.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KLH32_001545"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KLH32_001545"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBE0138417.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPLECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="KLH32_001546"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="KLH32_001546"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBE0138418.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="KLH32_001547"
     CDS             11233..12087
                     /locus_tag="KLH32_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0138419.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="KLH32_001548"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="KLH32_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBE0138420.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="KLH32_001549"
     CDS             complement(13550..14689)
                     /locus_tag="KLH32_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBE0138421.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KLH32_001550"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KLH32_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBE0138422.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="KLH32_001551"
     CDS             15875..17236
                     /locus_tag="KLH32_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBE0138423.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="KLH32_001552"
     CDS             17233..17751
                     /locus_tag="KLH32_001552"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBE0138424.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="KLH32_001553"
     CDS             17751..18071
                     /locus_tag="KLH32_001553"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBE0138425.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="KLH32_001554"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="KLH32_001554"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBE0138426.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="KLH32_001555"
     CDS             18890..20092
                     /locus_tag="KLH32_001555"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBE0138427.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="KLH32_001556"
     CDS             20189..20611
                     /locus_tag="KLH32_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE0138428.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="KLH32_001557"
     CDS             complement(20659..21531)
                     /locus_tag="KLH32_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBE0138429.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTTLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="KLH32_001558"
     CDS             complement(21543..22637)
                     /locus_tag="KLH32_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0138430.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="KLH32_001559"
     CDS             complement(22670..23668)
                     /locus_tag="KLH32_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0138431.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="KLH32_001560"
     CDS             complement(23693..25204)
                     /locus_tag="KLH32_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE0138432.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVTNGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="KLH32_001561"
     CDS             complement(25227..26210)
                     /locus_tag="KLH32_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBE0138433.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="KLH32_001562"
     CDS             complement(26307..29408)
                     /locus_tag="KLH32_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBE0138434.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="KLH32_001563"
     CDS             complement(29415..29588)
                     /locus_tag="KLH32_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138435.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="KLH32_001564"
     CDS             29706..30899
                     /locus_tag="KLH32_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE0138436.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KLH32_001565"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KLH32_001565"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBE0138437.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="KLH32_001566"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="KLH32_001566"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBE0138438.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KLH32_001567"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KLH32_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBE0138439.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KLH32_001568"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KLH32_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBE0138440.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KLH32_001569"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KLH32_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBE0138441.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KLH32_001570"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KLH32_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBE0138442.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KLH32_001571"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KLH32_001571"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBE0138443.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="KLH32_001572"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="KLH32_001572"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBE0138444.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KLH32_001573"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KLH32_001573"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBE0138445.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KLH32_001574"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KLH32_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBE0138446.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="KLH32_001575"
     CDS             45620..46468
                     /locus_tag="KLH32_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE0138447.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47304)
                     /locus_tag="KLH32_001576"
                     /pseudo
     CDS             complement(46807..47304)
                     /locus_tag="KLH32_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11281 a        12957 c        11892 g        11174 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgagcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg tcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tggtaacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagttcaacg catt
//
LOCUS       DAEEDS010000027        46385 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEEDS010000027 DAEEDS010000000
VERSION     DAEEDS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46385
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.027"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1014
                     /locus_tag="KLH32_001577"
     CDS             <1..1014
                     /locus_tag="KLH32_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HBE0138448.1"
                     /translation="NEQATQELASTSTRLSGGMYADAHAEWGVKPKILGVGGGVGIRG
                     GFKGSLDATDDDSHSASSGSRATHDARHDIDAKASKDFKEASDYFTSRKVSESGSHTD
                     NNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQMSEDLSQQFAQ
                     YVMKRAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQVQPGVDNAWRESRGDIGKGME
                     SVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRNDVKHQVDNMVTEYKGNIGDTQNSIH
                     GEENIVDRQYSELKNNHKQEEIQQNNRYNEENKRQILMPTPSEDALKQMVDDKKERLK
                     GPL"
     gene            1011..1520
                     /gene="traS"
                     /locus_tag="KLH32_001578"
     CDS             1011..1520
                     /gene="traS"
                     /locus_tag="KLH32_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBE0138449.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            1534..2265
                     /locus_tag="KLH32_001579"
     CDS             1534..2265
                     /locus_tag="KLH32_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBE0138450.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            2468..3205
                     /locus_tag="KLH32_001580"
     CDS             2468..3205
                     /locus_tag="KLH32_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138451.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            3256..5472
                     /gene="traD"
                     /locus_tag="KLH32_001581"
     CDS             3256..5472
                     /gene="traD"
                     /locus_tag="KLH32_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBE0138452.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGHQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQ
                     LPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            5472..10742
                     /gene="traI"
                     /locus_tag="KLH32_001582"
     CDS             5472..10742
                     /gene="traI"
                     /locus_tag="KLH32_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBE0138453.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            10762..11508
                     /gene="traX"
                     /locus_tag="KLH32_001583"
     CDS             10762..11508
                     /gene="traX"
                     /locus_tag="KLH32_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBE0138454.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            11567..12427
                     /locus_tag="KLH32_001584"
     CDS             11567..12427
                     /locus_tag="KLH32_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0138455.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            12530..13090
                     /locus_tag="KLH32_001585"
     CDS             12530..13090
                     /locus_tag="KLH32_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBE0138456.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            13219..13431
                     /locus_tag="KLH32_001586"
     CDS             13219..13431
                     /locus_tag="KLH32_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBE0138457.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            13676..14137
                     /locus_tag="KLH32_001587"
     CDS             13676..14137
                     /locus_tag="KLH32_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBE0138458.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTEMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            14183..14392
                     /gene="hha"
                     /locus_tag="KLH32_001588"
     CDS             14183..14392
                     /gene="hha"
                     /locus_tag="KLH32_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE0138459.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYGRIPKEIWKYVR"
     gene            14431..15021
                     /locus_tag="KLH32_001589"
     CDS             14431..15021
                     /locus_tag="KLH32_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBE0138460.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            15261..15509
                     /gene="repA"
                     /locus_tag="KLH32_001590"
     CDS             15261..15509
                     /gene="repA"
                     /locus_tag="KLH32_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBE0138461.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            15754..15828
                     /gene="tap"
                     /locus_tag="KLH32_001591"
     CDS             15754..15828
                     /gene="tap"
                     /locus_tag="KLH32_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBE0138462.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGRCD"
     gene            15821..16678
                     /gene="repA"
                     /locus_tag="KLH32_001592"
     CDS             15821..16678
                     /gene="repA"
                     /locus_tag="KLH32_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBE0138463.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            17380..17640
                     /locus_tag="KLH32_001593"
     CDS             17380..17640
                     /locus_tag="KLH32_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138464.1"
                     /translation="MAENGYGLAGQGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISICWWLCLKGYILYIKPDNTQIARQMSIMHPVIACL"
     gene            17747..18010
                     /locus_tag="KLH32_001594"
     CDS             17747..18010
                     /locus_tag="KLH32_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBE0138465.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSAQLRQQLQGRYQELRNEL"
     gene            18000..18299
                     /locus_tag="KLH32_001595"
     CDS             18000..18299
                     /locus_tag="KLH32_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0138466.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLVENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFIL"
     gene            complement(18335..19000)
                     /locus_tag="KLH32_001596"
     CDS             complement(18335..19000)
                     /locus_tag="KLH32_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138467.1"
                     /translation="MMFWNRNKKDKATIANEKKRFNHLLSVAEKLPVKALPDLIRAIV
                     RPVQSDFLLAVAEEGTDARPDMTPQEFFFKGLTQIQSYEKMKECEMNGADYPLSLASD
                     IVLPWPWSLPRYIDNVSRIGTHKGKPWKQDKNNHYVDLWLPWRIGFVHGGNHSITAGI
                     LAGEGSLIPEHVYDMSWMFELVRTDGNNWFVEGQKAEAVKSGRSAAVFEIGRLLVEGA
                     KER"
     gene            complement(19570..20133)
                     /locus_tag="KLH32_001597"
     CDS             complement(19570..20133)
                     /locus_tag="KLH32_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE0138468.1"
                     /translation="MDIIEKRITKRHLSESELSGVNYYNCIFERIQLDNFNFRDCEFE
                     KCRFVNCSIKNLKLNFFKLIDCEFKDCLLQGVNAADIMFPCTFSLVNCDLRFVDFISL
                     RLQKSIFLSSRFRDCLFEETDLRKSDFTGSEFNNTEFRHSDLSHCDFSMTEGLDINPE
                     INRILSIKIPQEAGLKILKRMGVVVGG"
     gene            complement(20137..20868)
                     /locus_tag="KLH32_001598"
     CDS             complement(20137..20868)
                     /locus_tag="KLH32_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE0138469.1"
                     /translation="MTIPLLEINSLSFSYKVNLPPVFNNLSLKIEQGELIGLLGENGA
                     GKTTLFNLIRGGVSNYEGTLKRNFSGGELVSLPQVINLSGTLRNEEVLDLICCFNKLT
                     KKQAWTELNHKWNDNFFIRYDKIRRKRTYTVSYGEKRWLIISLMLTLCKNARLFLLDE
                     PTVGIDIQYRMMLWELINKITADGKTVFFSTHIFDELTRDKIPFYMLSKNSINRYSDM
                     SDFIQSNNETTPEKAFIKEVMGTGD"
     gene            complement(20865..21590)
                     /gene="mcbE"
                     /locus_tag="KLH32_001599"
     CDS             complement(20865..21590)
                     /gene="mcbE"
                     /locus_tag="KLH32_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17 immunity protein McbE"
                     /protein_id="HBE0138470.1"
                     /translation="MVTLKMAIIFLMEQIRTPFSLIWTIMSPTVLFFFLHFNEIELHY
                     GDTAWLGKQISWFVGYISFSVVLFNYCLYLVGRRESGFIATFVHNMDGRLLFIRSQLI
                     ASLIMSILYVFFFILVVLTGFQASPDYQIVMIILKSIYINAFMMVSLTFMASFRVTFQ
                     TASTIYSVLITVCMVSGIVSLKYNEGIVYWINQVNPIAIYSTILQSDQELSLMTIFFY
                     SIMLIISIISALTFKTEPVWSSQ"
     gene            complement(21600..22790)
                     /locus_tag="KLH32_001600"
     CDS             complement(21600..22790)
                     /locus_tag="KLH32_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBE0138471.1"
                     /translation="MINVYSNLMSAWPATMAMSPKLNRNMPTFSQIWDYERITPASAA
                     GETLKSIQGAIGEYFERRHFFNEIVTGGQKTLYEMIPPSAAKAFTEAFFQISSLTRDE
                     IITHKFKTVRAFNLFSLEQQEIPAVIIALDNITAADDLKFYPDRDTCGCSFHGSLNDA
                     IEGSLCEFMERQSLLLYWLQGKANTEISSEIVTGINHIDEILLALRSEGDIRIFDITL
                     PGAPGHAVLTLYGTKNKISRIKYSTGLSYANSLKKALCKSVVELWQSYICLHNFLIGG
                     YTDDDIIDSYQRHFMSCNKYESFTDLCENTVLLSDDVKLTLEENITSDTNLLNYLQQI
                     SDNIFVYYARERVSNSLVWYTKIVSPDFFLHMNNSGAININNKIYHTGDGIKVRESKM
                     VPFP"
     gene            complement(22771..23589)
                     /locus_tag="KLH32_001601"
     CDS             complement(22771..23589)
                     /locus_tag="KLH32_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HBE0138472.1"
                     /translation="MSKHELSLVEVTHYTDPEVLAIVKDFHVRGNFASLPEFAERTFV
                     SAVPLAHLEKFENKEVLFRPGFSSVINISSSHNFSRERLPSGINFCDKNKLSIRTIEK
                     LLVNAFSSPDPGSVRRPYPSGGALYPIEVFLCRLSENTENWQAGTNVYHYLPLSQALE
                     PVATCNTQSLYRSLSGGDSERLGKPHFALVYCIIFEKALFKYRYRGYRMALMETGSMY
                     QNAVLVADQIGLKNRVWAGYTDSYVAKTMNLDQRTVAPLIVQFFGDVNDDKCLQ"
     gene            complement(23591..24478)
                     /locus_tag="KLH32_001602"
     CDS             complement(23591..24478)
                     /locus_tag="KLH32_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McbB family protein"
                     /protein_id="HBE0138473.1"
                     /translation="MVLPDIKKGKDMINILPFEIISRNTKTLLITYISSVDITHEGMK
                     KVLESLRSKQGIISEYLLDKLLDESLIDKDKGKEFLITTGVINKTKTSPLWVNSVIIS
                     DVPHLFSNAREQWKSDGVFVSHIIDIKDNNINVSDSTLIWLHLENYHSDIVKRIYSKF
                     ESNPGVAFIQSYYLKESFRIDGVYSPDLGTPCHFCHIERWLSREEKSFRRNEMSWANL
                     LQLLKKYQMTLPALALGESERGFSYHLIKRRLQELTGTSLVKSHVDNFMSSVSADLIT
                     CILCKEPVIHWQACSCLER"
     gene            complement(24505..24714)
                     /locus_tag="KLH32_001603"
     CDS             complement(24505..24714)
                     /locus_tag="KLH32_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17"
                     /protein_id="HBE0138474.1"
                     /translation="MELKASEFGVVLSVDALKLSRQSPLGVGIGGGGGGGGGGSCGGQ
                     GGGCGGCSNGCSGGNGGSGGSGSHI"
     gene            25244..25696
                     /locus_tag="KLH32_001604"
     CDS             25244..25696
                     /locus_tag="KLH32_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0138475.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISITLGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPIENKGIYAGNPARLIRIL"
     gene            complement(26099..26449)
                     /locus_tag="KLH32_001605"
     CDS             complement(26099..26449)
                     /locus_tag="KLH32_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBE0138476.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADLNSSTIIQSSSQSEQK
                     SDEETKKNAMKLIN"
     gene            complement(26452..27414)
                     /gene="parM/stbA"
                     /locus_tag="KLH32_001606"
     CDS             complement(26452..27414)
                     /gene="parM/stbA"
                     /locus_tag="KLH32_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBE0138477.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            27925..28851
                     /locus_tag="KLH32_001607"
     CDS             27925..28851
                     /locus_tag="KLH32_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBE0138478.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLF
                     LAGSAGLLQITENIRSEQCPGVTAAGRGAVSLENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVTHYGG"
     gene            28848..29159
                     /locus_tag="KLH32_001608"
     CDS             28848..29159
                     /locus_tag="KLH32_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138479.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARGGRFYRSRLASVKVYASPSPFTDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            29236..29919
                     /locus_tag="KLH32_001609"
     CDS             29236..29919
                     /locus_tag="KLH32_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBE0138480.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGVELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            29920..30141
                     /locus_tag="KLH32_001610"
     CDS             29920..30141
                     /locus_tag="KLH32_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138481.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            30155..30589
                     /locus_tag="KLH32_001611"
     CDS             30155..30589
                     /locus_tag="KLH32_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBE0138482.1"
                     /translation="MYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            30634..31404
                     /locus_tag="KLH32_001612"
     CDS             30634..31404
                     /locus_tag="KLH32_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138483.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLGAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            31820..32245
                     /locus_tag="KLH32_001613"
     CDS             31820..32245
                     /locus_tag="KLH32_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBE0138484.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            32292..32714
                     /locus_tag="KLH32_001614"
     CDS             32292..32714
                     /locus_tag="KLH32_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBE0138485.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRYIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            32711..32902
                     /locus_tag="KLH32_001615"
     CDS             32711..32902
                     /locus_tag="KLH32_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138486.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            33937..34167
                     /locus_tag="KLH32_001616"
     CDS             33937..34167
                     /locus_tag="KLH32_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138487.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            34219..35580
                     /locus_tag="KLH32_001617"
     CDS             34219..35580
                     /locus_tag="KLH32_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBE0138488.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            35628..36191
                     /locus_tag="KLH32_001618"
     CDS             35628..36191
                     /locus_tag="KLH32_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0138489.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            36217..36453
                     /locus_tag="KLH32_001619"
     CDS             36217..36453
                     /locus_tag="KLH32_001619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138490.1"
                     /translation="MPVCPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMLLFLLLLLLLLLLLLLLLLLLLLLFFFFLLL"
     gene            37096..37635
                     /locus_tag="KLH32_001620"
     CDS             37096..37635
                     /locus_tag="KLH32_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE0138491.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            37698..37931
                     /locus_tag="KLH32_001621"
     CDS             37698..37931
                     /locus_tag="KLH32_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBE0138492.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            37997..39955
                     /locus_tag="KLH32_001622"
     CDS             37997..39955
                     /locus_tag="KLH32_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBE0138493.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVILQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVGGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGVAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NCWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            40010..40444
                     /gene="psiB"
                     /locus_tag="KLH32_001623"
     CDS             40010..40444
                     /gene="psiB"
                     /locus_tag="KLH32_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBE0138494.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            40441..41160
                     /locus_tag="KLH32_001624"
     CDS             40441..41160
                     /locus_tag="KLH32_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBE0138495.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(41172..41360)
                     /locus_tag="KLH32_001625"
     CDS             complement(41172..41360)
                     /locus_tag="KLH32_001625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HBE0138496.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            41440..41598
                     /locus_tag="KLH32_001626"
     CDS             41440..41598
                     /locus_tag="KLH32_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE0138497.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            42289..42495
                     /locus_tag="KLH32_001627"
     CDS             42289..42495
                     /locus_tag="KLH32_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE0138498.1"
                     /translation="MYIPQNFLPRSRQKLLRSAPGQSFPSVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSTDDGG"
     gene            42520..42807
                     /locus_tag="KLH32_001628"
     CDS             42520..42807
                     /locus_tag="KLH32_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138499.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            42926..43747
                     /locus_tag="KLH32_001629"
     CDS             42926..43747
                     /locus_tag="KLH32_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBE0138500.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(44044..44646)
                     /locus_tag="KLH32_001630"
     CDS             complement(44044..44646)
                     /locus_tag="KLH32_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBE0138501.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            44977..45360
                     /locus_tag="KLH32_001631"
     CDS             44977..45360
                     /locus_tag="KLH32_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBE0138502.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYETQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPENDEG"
     gene            45494..46171
                     /locus_tag="KLH32_001632"
     CDS             45494..46171
                     /locus_tag="KLH32_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBE0138503.1"
                     /translation="MRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKKRKILYANGA
                     FIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIYQIRME
                     NVSFYNEESVVLWQINIFPDYPFFRVEKENYYHRDSYVQSVISNMTAKSLVVFCFYAL
                     GYKHINIAKELKITEVASKKRIKKINADINKRLGSFDLFRNKCIKTGVMYEILNVLSE
                     YMGVKKM"
     gene            46259..46369
                     /locus_tag="KLH32_001633"
                     /pseudo
     CDS             46259..46369
                     /locus_tag="KLH32_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
BASE COUNT        11593 a        11031 c        12536 g        11225 t
ORIGIN      
        1 aatgagcagg cgactcagga actggcatca acaagtacca ggctatctgg tggaatgtat
       61 gctgatgctc atgctgaatg gggggttaaa cctaaaattc ttggtgttgg tggtggggta
      121 ggcatcagag gtggatttaa ggggagcctg gatgcaacgg atgatgactc acattcagca
      181 agtagtggca gcagggctac ccatgatgct cgccatgata tcgatgctaa agccagtaag
      241 gactttaaag aggccagcga ttactttacc agtcgcaaag tcagtgaatc cggcagccat
      301 actgacaata atgccgattc ccgcgtggac cagttgtctg cagccctgaa ctcagcaaaa
      361 cagagttacg accagtacac gacgaatatg acccgcagcc atgaatatgc ggaaatggcc
      421 tcccgtactg aaagcatgag cgggcagatg agtgaagact tgtcgcaaca gtttgcacag
      481 tatgtgatga aacgcgcacc gcaggatgca gaagctattc tgacaaatac cagttcgccg
      541 gagattgcag aacgccgtcg ggctatggcg tggtcttttg tgcaggaaca ggtacagcct
      601 ggtgttgata atgcctggcg tgaatcccgt ggggatattg gtaaaggaat ggagagcgta
      661 ccttcgggtg gtggcagcca ggatattatt gctgaccatc aggggcatca ggccattatt
      721 gatcaaagaa cgcaggacag taatatccgt aatgatgtaa aacatcaggt tgataatatg
      781 gtcacagaat ataaagggaa tatcggagat actcagaata gtattcatgg cgaagaaaat
      841 attgtagaca gacaatattc agagttaaaa aataatcaca aacaggaaga aattcagcaa
      901 aacaacagat ataatgaaga aaataaacga cagatattaa tgccaacacc gtctgaagat
      961 gcgttaaaac agatggtgga tgataagaaa gaacgattga aggggccatt atgatcactc
     1021 actatgatat aaaaatggag atgcagaaac taaaggaggt actctctgta gagggtgtta
     1081 atatcccatc attattacaa gtcataaagc cgggaactta tgtgttttta tgggttttat
     1141 tatggccaac ttttctgcgt ttagtatcgg ttaaaagtga tgtcagggat gttggctttg
     1201 atatatgtgc tagtggaatg atggggttcc tgctttttgt ggctattaca aatggaatga
     1261 tgttatacct tgcgattcct gatagtttca gaaaggattc aaaaataata aattttatgt
     1321 attctaagag taagacatat attttgttgt ttttgattgt tttttctatg gtttcgttta
     1381 tgcattctat cttatatgtt tttgcactaa tgattacatt catattattt ttcttggtgt
     1441 ataccataga tataaatcgt tataatctct cagctatagc ctcagtaata ggactattca
     1501 aaaaagagtc tgttagttaa aaggatattt tggatgaaaa caaaaaaatt gatgatggtt
     1561 gcactggtca gttccactct ggccctttca gggtgtggtg cgatgagcac agcaatcaag
     1621 aagcgtaacc ttgaggtgaa gactcagatg agtgagacca tctggcttga acccgccagc
     1681 gaacgcacgg tatttctgca gatcaaaaac acgtctgata aagacatgag tgggctgcag
     1741 ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg tggtgacttc tccggataaa
     1801 gcctactact ggattcaggc gaatgtgctg aaggccgata agatggatct gcgggagtct
     1861 cagggatggc tgaaccgtgg ttatgaaggc gcagctgttg gtgcagcgtt aggtgccggt
     1921 attaccggat ataactcaaa ttctgccggt gccacactcg gtgtaggcct tgctgctggt
     1981 ctggtgggta tggctgcaga tgcgatggtg gaagatgtga actataccat gatcacggat
     2041 gtacagattg cagagcgtac taaggcaacg gtgacaacgg ataatgttgc cgccctgcgt
     2101 cagggcacat ccggtgcgaa aattcagacc agtactgaaa cgggtaacca gcataaatac
     2161 cagacccgtg tggtctcaaa tgcgaacaag gttaacctga aatttgaaga ggcgaagcct
     2221 gttctcgaag accagctggc caaatcaatc gcaaatattc tctgaacctc gtcaggaggc
     2281 tgatctgacc ggcctccttg ctgacgcctc gcgttgctcg ttgtccaacc ccgggatgta
     2341 acaaaataaa ttttgtcacc tcccggcgtt tccgaaataa atatgaaaac actgagcggt
     2401 tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga agccgggatc ttccggagaa
     2461 agtcattatg acatcagaca atatcgctga atatgttggt gagaataatc aatttactga
     2521 gaagcctgta actgaatacc ccccggtgat acaggataat acagtgacca ctaaagacta
     2581 tctgtacagc aataatattg atactgacct tttagtggtt atcggagggc ttattgttat
     2641 attgtttctc atgatgctta tcatgtactt tattaattct ctcaggcata aggaattaac
     2701 tgaaatactg acaaaaatat cttttcctga aaaatccggg agcaatttgc ttaaaccgga
     2761 ggcggtcagc tgtaatttgt tgtccctgtg tgtgctggca ccggtattct gtacacccag
     2821 aaagctggct gaagtttcca gggagatcat cgtggcgagt gcgcaggaaa ttgcatggac
     2881 gccggggagc agtcgtattg atggccacca ggcggagaat atcacctggg cgatttcatc
     2941 gcagtctcct gccgataagt atgatctcat caataaaacg tcctgtgttt ctcctgacag
     3001 tggccgtttt tatgaaccgg acacagttct ggatcaccgt gaacgggtac gcctttttat
     3061 tcagatccag tttccgcatg ggagtgactg gtctgaagta ttgcgcctga ctgacattgt
     3121 tacagagaag gtctcttccg gcaggactgc aggcgggcat caccaccggc atacagggta
     3181 cacctggtct gtttctgtga attaacccca catcatcctt ttctgccctg ttccagattt
     3241 tcccggagtt tcatcatgag ttttaacgca aaggatatga cccagggcgg tcagattgcg
     3301 tccatgcgta tccgcatgtt cagccagatc gccaatatca tgctttactg cctgtttatt
     3361 tttttctgga tcctcattgg tctggtctta tgggtaaaaa taagctggca gacgtttatc
     3421 aacggctgta tttactggtg gtgtaccacg ctggaaggca tgcgggattt aataaaatcc
     3481 cagccggtct atgagatcca gtattacggc aaaaccttcc ggatgaacgc tgcccaggta
     3541 ctgcatgata aatatatgat ctggtgcgga gagcagctat ggtccgcatt cgttctggcc
     3601 acagttgtgg cactggttat ttgcctgatc accttctttg ttgtctcctg gattctgggg
     3661 catcagggga aacagcagag tgaaaatgag gtgaccggcg ggcgtcagct gacggataac
     3721 ccgaaagaag tggcccggat gctgaaaaaa gacggcaagg actccgatat ccggattggc
     3781 gacctgccga ttatccggga ttccgaaatc cagaacttct gtctgcacgg cacggtcggg
     3841 gccggtaagt cggaggttat ccgtcgtctg gccaactacg cccgtcagcg cggtgatatg
     3901 gtggtgattt atgaccgttc aggggaattt gttaaaagtt actatgatcc ctccatcgat
     3961 aaaatcctga atccgctgga tgcccgctgt gccgcatggg atttgtggaa ggagtgcctg
     4021 acacagccgg attttgataa taccgcgaac acgctgatcc ctatgggtac aaaagaggac
     4081 ccgttctggc agggttcagg acgtaccatt tttgcggaag cggcgtacct gatgcgtaat
     4141 gaccccaacc gcagctacag caaactggtg gacacactgc tttccatcaa aattgaaaaa
     4201 ctgcgtacct tcctgcgtaa ttcaccggca gccaacctgg tggaagagaa aattgagaaa
     4261 acggcgattt ccatccgtgc tgtgctgacc aactacgtga aagccatccg ttacctgcag
     4321 gggattgagc ataacggtga gtcttttacc atccgtgact ggatgcgtgg tgtccgggaa
     4381 gatcagaaaa acggctggct gtttatttcg tcgaacgccg acacccatgc ctccctgaag
     4441 ccggtgatct ccatgtggct gtccattgcc attcgtggtc tgctggcaat gggagaaaac
     4501 cgtaaccgtc gtgtgtggtt tttctgtgac gagttaccca cgttacacaa actgccggac
     4561 ctggtggaga tcctgccgga agcccgtaag ttcggtggct gttatgtgtt tggtatccag
     4621 tcctatgccc agctggaaga tatctacggt gagaaagcgg cggccacgct gtttgacgtc
     4681 atgaacaccc gtgccttttt ccgttccccg agccataaga ttgcagagtt cgctgccggt
     4741 gaaattggtg agaaagagca cctgaaagcc agcgagcagt attcctacgg tgctgacccg
     4801 gtacgtgacg gggtatcgac cggtaaggat atggagcgtc agacgctggt cagttattcc
     4861 gacattcagt ctctgccgga tctgacctgc tatgtcactc tgcccggacc gtatccggca
     4921 gtaaaactct ctctgaaata tcaggcacga ccgaaggtcg ctccggagtt tattccgcgt
     4981 gacatcaacc cggaaatgga gaaccgtctg agtgccgtac ttgctgcaag ggaagcagaa
     5041 ggtcgccaga tggccagcct cttcgaaccg gaggttgcct ccggagaagg cgtgactcag
     5101 gctgaacagc cacaacagcc acaacagcca caacagccac aacagccaca acagccacaa
     5161 cagccacaac agccacaaca gccacaacag ccagtgtctt ctgtcatcaa tgataagaag
     5221 tcagatgcag gtgtgagtgt tccggcaggg gggatcgagc aggagctgaa aatgaaaccg
     5281 gaagaagaga tggaacagca actgccaccc gggatcagtg aatccggtga agtggtggat
     5341 atggccgctt atgaggcatg gcaacaggaa aatcatccgg acatccagca gcagatgcag
     5401 cgtcgtgaag aggtgaacat taatgtgcac cgggagcgtg gggaggatgt tgagccggga
     5461 gatgatttct gatgttgagt ttcagcgttg ttaaatcggc aggcagtgcc ggaaactatt
     5521 acaccgacaa ggataattac tatgtgctgg gcagcatggg agaacgctgg gctggtcagg
     5581 gggctgaaca gcttgggctg cagggcagcg tcgataagga tgtttttacc cgtcttctgg
     5641 agggcaggct gccggacggg gcggatttaa gccgcatgca ggatggcagt aataagcatc
     5701 gtcccggcta cgacctgacc ttctccgccc ccaaaagtgt ctccatgatg gccatgctcg
     5761 ggggggataa acgtcttatt gatgcacata accaggccgt ggattttgct gtccgtcagg
     5821 tggaggcgct ggcttccaca cgggtgatga cggacggaca gtcagaaacg gtgctgaccg
     5881 gtaatctggt gatggcactg tttaaccatg acaccagtcg cgatcaggac ccacagttac
     5941 acacgcatgt agtggtggct aatgtcacgc agcataacgg cgagtggaag acactgagca
     6001 gtgacaaagt ggggaaaacc ggattttccg agaacgtgct agcgaaccgg attgcgttcg
     6061 ggaaaatata tcagagtgag ttacgtcagc gcgttgaggc gctgggttat gaaaccgaag
     6121 tggtcggtaa gcacggtatg tgggagatgc cgggcgtacc ggtggaggcc ttttccagtc
     6181 gttcacaggc tatcagggag gccgtcggtg aagacgcctc gctgaaatcc cgggatgtgg
     6241 cggcactgga tacgcgaaaa tccaaacagc acgtcgatcc ggaaatcaga atggctgaat
     6301 ggatgcagac gctgaaggaa accgggttcg acatccgggc atatcgtgac gcggcggatc
     6361 agcgtgcgga gatccgcacg caggcgcccg ggcctgcttc acaggacggg ccggatgtgc
     6421 agcaggcggt gacacaggcg attgccggat taagtgaacg aaaagtgcag ttcacgtata
     6481 cggacgtgct ggccaggacg gtcggcatac tgccgccgga aaatggtgtg attgaacggg
     6541 cacgcgccgg tatcgatgag gccatcagcc gtgagcagct tatccccctc gaccgtgaga
     6601 aggggctgtt cacatcagga attcatgtgc tcgatgagct gtccgtccgg gcactcagtc
     6661 gtgacatcat gaaacagaac cgggtgaccg tacatccgga gaaaagtgtc ccccggacgg
     6721 ctggttacag cgatgcggtc agtgttctgg cacaggaccg tccgtcgctg gccattgtgt
     6781 ccgggcaggg cggtgcagcc gggcagcgtg agcgggtggc tgaactggtc atgatggccc
     6841 gggagcaggg gcgggaggtg cagattatcg ctgctgaccg tcgctcgcag atgaacctga
     6901 agcaggatga acggctgtcc ggtgagctga taaccggacg tcgtcagctg caggagggca
     6961 tggtcttcac gccgggcagt actgttatcg tcgaccaggg cgaaaaactc tccctgaaag
     7021 agacgttaac cctgctggac ggtgccgcac gtcataacgt acaggtcctg ataaccgaca
     7081 gcgggcagcg aaccggtaca ggcagtgcgc tgatggccat gaaggatgcc ggggtgaaca
     7141 cgtatcgctg gcagggcgga gaacagcgac cggcaaccat catcagtgaa ccggaccgga
     7201 atgtccgcta tgaccggctg gccggagatt ttgcggccag cgtgaaagcc ggagaagaga
     7261 gcgtggcaca ggtcagcggg gtacgggaac aggccatact gacacaggcc attcgcagtg
     7321 agctgaaaac acagggcgtg ctcggacacc cggaggtgac catgaccgcc ctgtcaccgg
     7381 tctggctgga cagccggagc cgttatctgc gggatatgta ccgaccgggg atggtgatgg
     7441 agcagtggaa cccggagaca cgcagtcatg accgctatgt tatcgaccgg gtgacggcgc
     7501 agagtcacag cctgaccctg cgggatgcgc agggcgaaac gcaggtggtg cgtatttcct
     7561 ccctggacag cagctggtcg ctgttccggc cggaaaaaat gccggtggca gacggcgagc
     7621 gactgagggt gacagggaaa attcccggac tccgcgtctc cggcggtgac cgcctgcagg
     7681 tggcatccgt cagtgaagat gcgatgacgg ttgttgtgcc ggggcgggct gaaccggcca
     7741 ccctgcctgt ggctgattca ccgttcacgg cactgaagct ggagaacggc tgggtggaaa
     7801 cgcccgggca ttccgtcagt gacagtgcga cggtgtttgc ctccgtcaca cagatggcaa
     7861 tggacaacgc caccctgaac ggtctggccc gcagcggtcg tgatgtccgg ctgtattcct
     7921 cactggatga aacccgtact gcggaaaaac ttgcccgcca tccctccttt acggtggttt
     7981 ctgagcagat aaaggcgcgg gccggtgaga catcgctgga aaccgctatc agtctgcaga
     8041 aaaccgggct gcacacgccg gcacagcagg ccattcatct ggcccttcct gtgctggaaa
     8101 gtaaaaacct ggccttcagc atggtggacc tgctgacaga ggcgaagtcg tttgctgcag
     8161 aaggaaccgg ttttactgaa ctgggagggg aaatcaatgc gcagataaaa cgcggtgact
     8221 tactgtatgt ggatgtggca aaaggctatg gcacaggcct gctggtttcc cgtgcgtcgt
     8281 atgaggcaga aaaaagtatt cttcgccata ttctcgaagg taaggaggcg gtcacgccgc
     8341 tgatggagag agtacccggc gaactcatgg agacgttaac gtcgggacag cgtgccgcca
     8401 cccgtatgat actggaaacg tccgaccgtt tcacggtggt acagggttat gccggtgtgg
     8461 gtaagaccac acagttccgg gcggtgatgt cagccgtgaa catgctgccg gcgagtgagc
     8521 gtccccgagt tgtggggctg gggcccacgc accgtgcggt cggtgagatg cgcagcgccg
     8581 gcgtggatgc acagacgctg gcgtcctttc tgcatgacac gcagctgcag cagcgcagcg
     8641 gagaaacgcc ggatttcagc aacacgctgt tcctgctcga tgagagctca atggtgggca
     8701 ataccgacat ggcacgggca tacgccctga ttgcggccgg tggcggtcgt gccgtggcca
     8761 gtggtgacac ggaccagctg caggccatcg cgcccggtca gcctttccgt ctccagcaga
     8821 cgcgcagtgc tgccgatgtg gtcatcatga aggagattgt gcgtcagacg ccggaactgc
     8881 gggaggcggt atacagcctg attaaccggg atgtggaaag ggcactgtcc gggcttgaga
     8941 gtgtgaaacc gtctcaggtt ccccgtcagg agggcgcatg ggcaccggag cactccgtga
     9001 cggagttcag tcacagccag gaagcgaaac tggcagaagc gcagcagaag gcgatgctga
     9061 aaggtgagac ttttccggat gtccccatga cactgtatga agccattgtc cgcgactata
     9121 ccggcaggac accggaagca cgggagcaga cgctgattgt cacgcacctg aatgaggacc
     9181 ggcgcgtact gaacagcatg attcatgatg cacgggaaaa ggccggtgag ctggggaaag
     9241 agcaggtcat ggtgcctgtc ctgaacacag cgaatatacg tgacggggag ctgcgtcgtc
     9301 tctccacctg ggagaataac ccggatgccc ttgccctggt ggatagtgtg tatcaccgga
     9361 ttgccggtat cagcaaggat gacgggctga taaccctgga ggatgcggaa ggtaacacgc
     9421 ggctgatttc gccccgggag gcggtggctg aaggtgtcac actgtacacc ccggacaaaa
     9481 tccgggtggg gaccggcgac cggatgcgct tcacgaagag tgaccgggag cgcggttatg
     9541 tggccaacag cgtctggacg gtgacagcgg tttccggtga cagtgtcacg ctgtcagacg
     9601 gacagcagac ccgggtgatt cgccccggcc aggagcgggc agagcaacat attgacctgg
     9661 cttatgccat caccgcccac ggtgcgcagg gggcgagtga aacctttgcc atcgcgcttg
     9721 aaggcacgga aggtaaccgg aaactgatgg ccggctttga gtcagcctac gtggccctgt
     9781 cgcgtatgaa gcagcatgtg caggtgtaca ccgataaccg tcagggctgg acggatgcca
     9841 ttaacaatgc cgtacagaaa ggaactgccc acgatgtgct ggagccgaaa ccggaccggg
     9901 aggtcatgaa tgcgcagcgg ctgttcagta cggcacggga actgcgggac gtggcggcag
     9961 gccgtgccgt tctccgtcag gcagggctgg ccgggggaga cagccctgca cggtttattg
    10021 caccgggacg taaatatccg cagccgtatg tggcactgcc ggcgtttgac cgtaacggca
    10081 agtcagctgg tatctggctg aacccactga ccacggatga cggaaacggg ctgcggggat
    10141 tcagtggtga aggacgggtg aaaggcagcg gggatgcgca gttcgtggcc ctgcagggca
    10201 gccgtaacgg agagagcctg ctggctgata atatgcagga tggcgtccgg attgcccgtg
    10261 ataatcctga cagcggtgtg gtggtgagaa ttgccggtga aggtcgtccg tggaatcccg
    10321 gtgccatcac cggtggccgc gtgtgggggg atatcccgga taacagcgtc cagccggggg
    10381 ccggaaatgg cgaaccggtc acggcagagg tactggcaca gcggcaggct gaagaggcca
    10441 tccgccgtga aacggaacgc cgcgcagatg aaattgtcag gaaaatggca gagaacaaac
    10501 ctgacctgcc ggatggcaaa acagagctgg ctgtcaggga tattgccgga caggagcgtg
    10561 accggtctgc catatctgaa cgggaaaccg cactgccgga gagtgtgctg cgtgaatcac
    10621 aacgggagcg ggaagcggtc cgggaggttg cccgggaaaa tctgctgcag gagcgtctgc
    10681 agcagatgga gcgggatatg gttcgtgacc tgcagaaaga gaaaaccctg ggcggagact
    10741 gatacaggaa gacaaacact gatgacaacg gataacacga acacgacacg taacgattca
    10801 ctggctgcac ggaccgatac ctggttgcag tcgtttctgg tctggtcacc cggacagcgg
    10861 gatatcatca aaacggtggc gctggtgctg atggtactgg accacatcaa tctgatattc
    10921 cagctgaagc aggaatggat gtttctggcc gggcgaggag cctttccgct gtttgccctg
    10981 gtgtgggggc tgaatctgtc ccgtcatgcg catatccggc aaccggccat taaccggctg
    11041 tggggatggg gaattattgc ccagttcgca tattatctgg ccggctttcc ctggtatgag
    11101 gggaatatcc tgtttgcctt tgcagtggcg gcacaggtgc tgacgtggtg tgaaacgcgc
    11161 agtgggtggc gtacagcagc ggccattctt ctgatggccc tgtgggggcc tttgtccggc
    11221 accagttacg gcattgccgg gctgctgatg ctggcggtca gttaccgact gtaccgggcg
    11281 gaagacagag cggaacgtct ggcgctgctt gcatgcctgc tggccgttat tccggcactt
    11341 aaccttgcct ccagtgatgc agcggcggtg gccggtcttg taatgacggt gctgaccgtt
    11401 ggtctggtgt cgtgtgcagg aaaatcatta ccccgtttct ggcccgggga ttttttcccg
    11461 gtattctacg cctgtcatct ggctgtgctg ggcgttctgg cgttgtgact ggtatgatac
    11521 tgacgctgat tctgattatt aataaacaac ataacacagg atatttatga aaattacgga
    11581 tcacaaactc tctgaaggta ttgccctgac atttcgtgtt cctgaaggaa atataaaaca
    11641 tcctttgatt atcttatgcc atgggttttg cggtatcagg aatgttctgc tcccttgctt
    11701 tgcgaatgcc ttcacagaag caggattcgc aacgattact tttgattatc gaggattcgg
    11761 tgagagtgat ggagaacgag ggcgtctggt tcctgcaatg cagaccgaag atattatttc
    11821 agttatcaat tgggcagaaa agcaggaatg tattgataat cagcgtattg ggttatgggg
    11881 aacatcgctg ggaggggggc atgtcttcag tgcagctgcg caggaccaac gcgtaaaatg
    11941 tattgtcagc cagctggctt ttgctgacgg tgatgttctg gttaccggag agatgaatga
    12001 atcggagaga gcgtcttttc tgtccacgct gaataaaatg gcagagaaga agaaaaacac
    12061 cgggaaagag atgtttgtgg gggttacccg ggtactgagt gataatgaat caaaggtatt
    12121 cttcgagaag gtaaaaggtc agtatcctga aatggatata aaaatacctt tccttacggt
    12181 tatggagacg ttgcaatata aaccggcaga gagcgcagcc aaagtacagt gcccagtttt
    12241 gattgttatt gccggccagg atagtgttaa tccaccagaa cagggaaaag ccttatatga
    12301 tgctgttgct tctggtacta aggaattata cgaggaagct gacgcatgcc attatgatat
    12361 ttatgaaggc gcgttttttg aacgtgtggc ggcagtacag acgcaatggt ttaaaaaaca
    12421 tctttaattg tcggtattaa tatgtcagag gtcgttgagg tcagcttaat atcatagtga
    12481 gtagtttcaa cgacctggag gacatatttt cacaaaggtc agggcaatca tgacagagca
    12541 gaaacgaccg gtactgacac tgaaacggaa aacggaaggg gaaacaccga cccggagccg
    12601 gaaaaccatc atcaatgtca ccacgccacc aaaatggaag gtgaaaaagc agaaactggc
    12661 ggagaaggct gcccgggaag cagagctgac agcaaaaaaa gcgcaggcca gacaggcgct
    12721 gtccatttat ctgaacctgc cctcgttgga tgaggccgtg aacaccctga aaccctggtg
    12781 gccgggatta tttgacggtg acacaccccg acttctggcc tgcggtatcc gggacgtgtt
    12841 actggaagac gtggcgcagc ggaatatccc gctctcgcat aaaaaactgc gcagggcgct
    12901 gaaggccatc acccgttcag aaagctatct gtgtgccatg aaagccggtg cctgccggta
    12961 tgacacggaa gggtatgtga cggagcatat ttctcaggag gaagaagtgt atgcggcaga
    13021 gcgtctggat aaaatccgcc gccagaaccg gataaaggca gaacttcagg ccgtgcttga
    13081 tgaacaataa aaaagcctcc ttcacactaa aggaggcgga aaaataaggc ttctctgaaa
    13141 ccttcttcca tacccggcag aagaaggtta taaaaattta taacacattc accggcctgg
    13201 attaaagggg gaaggaaaat gaacggaaca gggcgtgatt cgcaaaactc cggggtatgg
    13261 cgctaccggc aggtcggacg ccgggctgtc cggctggaag tcagggggga ctggccggga
    13321 gccgctgagg catggctacg ggctgcgcag tctgctcccc ggacagactg gcagcagttt
    13381 gcccgaaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaagc
    13441 agccttcatt actccaggga ttcagaaggc tgaataatgc atgagatttg ctttttttaa
    13501 tatccggtcg ggaagtatga ccggacagtt ctgaatagca cagagttttt tccgtattaa
    13561 gtcgtgattt tatattttgt gattaatttc acaaaataag gtgttattca gtgtgtgctg
    13621 cgatattcag gattacctga aatacaggtg ccatttatct gatatggaga ataacatgag
    13681 gaaatatatt cctctggtat tatttatctt ttcatggccg gtattgtgtg cagatattca
    13741 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cacggaaggc
    13801 agtgcggatc aggctgataa atattgatgc accggaaaag aaacaggatt atggccggtg
    13861 gtcaacggaa atgatgaaat cactggttgc aggaaaaacg gttaccgtca catattttca
    13921 gcgagatcgt tatggtcgta ttctcgggca ggtttatgca ccagacggaa tgaatgttaa
    13981 tcagtttatg gtccgggccg gggctgcctg ggtatatgag cagtacaaca cagatcccgt
    14041 gctgcctgta cttcagaacg aagccagaca acaaaaacgt gggctctggt cagatgctga
    14101 tccggttccc ccctggatat ggagacaccg taaataaagc gatggcaatc ttatcattca
    14161 tctgttctca gcgggaataa aaatggcaaa aacaaaacag gaatggctgt atcagttacg
    14221 gcgatgttca tcagtcaata cgctggaaaa aatcattcat aaaaacagag acagtttatc
    14281 gaccagtgaa cgtgaatcat ttaattcagc agcagaccac cggttagcgg aattaatcac
    14341 cggaaagtta tatggcagga taccaaaaga gatatggaaa tatgtaaggt aataattcag
    14401 caataattaa ttaccaggag cagataaaca atgaagctga ttatttttat tctcattgtt
    14461 ctgattattg cagcgttact tatccggatt atcctcagaa gtgtaaatca gcattctcct
    14521 ttgctgatgc aattgcatgc ggcaggtata cggaccggtg atgcagagcg aatactgtcc
    14581 ggcggtgaat actggcaacg tcagaagacg ctgctgacag aaagggaagt cagttttatg
    14641 aaaggactgt tcagaattgt ggatatgaag cggtggtatc tgtgtccgca ggtacgggtc
    14701 gcagatatcg tccagctgaa cgggaatatc cggccacgat cacgccagtg gtggcagtta
    14761 ttcaggatgg tgtctcagtg gcatgttgat gtggttatcg ttgagcggcg ttcgttcagt
    14821 attgttgcag cagtagagct ggatgatgcc agccatttac gacctgaacg cagacgccgg
    14881 gatattcttc tggaagaggt tctgaggcag gctggtattc cgttgctcag aagccacgat
    14941 gccagaaaac tgctgcagat gaccggagaa tggctgaata caacaggggc tgcccagcag
    15001 tcaccggaac atcgtagctg acgccttcgc gttgctcagt tgtccaaccc cggaaacggg
    15061 aaaaagcaag ttttccccgc tcccggcgtt tccataactg aaaaccatac tatttcacag
    15121 tttaaatcac attaaacgac agtaatcccc gttgatttgt gcgccaacac agatcttcgt
    15181 cacaattctc aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt
    15241 ttgagtattg aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc tcatcaggta
    15301 caaagcgagc atacagaaaa ggtaatcctc tgacgcttgc agaacgtcag caggcatccc
    15361 tggcaagaaa acgagctaca cataaagaac tcagagtgtt cattcctgct gcgttaaaag
    15421 cgcaattgca ggtgatgtgt gaagctgagg gcgttaccca ggctgagatg attgccgaac
    15481 tgataaagca gaagagtgct tttagctaac taaaatgtag cgacatcaca ttcttgcctg
    15541 tttctggtgt gcgtgataga ttgctgatcg tttaaggaat tttgtggctg gccacgccgt
    15601 aaggtggcag ggaactggtt ctgatgtgga tttacaggag ccagaaaagt gaaaaccccg
    15661 ataatcttct ctaactttgg cgagtacgaa agattaacgg ggctaacaag aaactgcata
    15721 gaagctgttg ctctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt
    15781 ctgtgctcac tccttctgtg caacataagt gcaggaaggt gtgactgatc ttcaacaaac
    15841 gtattaccgc caggtaaaga acccgaatcc ggtgtttaca ccccgtaaag gtgccggaac
    15901 gctgaagttc tgcgaaaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt
    15961 cgccattcat gtggcgcatg cccgttcgcg tggtctgcgc cggcgcatgc caccggtgct
    16021 gcgtcgacgt gccattgatg cgctgctgca ggggctgtgt ttccactatg acccgctggc
    16081 caaccgcgtc cagtgctcca tcaccacgct ggccattgag tgcggactgg cgacagagtc
    16141 cggtgccgga aaactctcca tcacccgtgc cacccgtgcc ctgacgttcc tggctgagct
    16201 ggggttgatt acctaccaga cggaatatga cccgcttatc ggctgctaca ttccgaccga
    16261 catcacgttc acaccggcac tgtttgccgc tctcgatgtg tctgaggatg cggtggcagc
    16321 tgcgcgccgc agtcgtgttg aatgggaaaa cagacagcgc aaaaagcagg ggctggatac
    16381 cctgggtatg gatgagctaa tagcgaaagc ctggcgtttt gtgcgtgagc gtttccgcag
    16441 ttaccagaca gagcttaagt cccgtggaat aaaacgtgcc cgtgcgcgtc gtgatgcgaa
    16501 cagggaacgt caggatatcg tcaccctggt gaaacggcag ctgacgcgtg aaatctcgga
    16561 agggcgcttc actgctaatg gtgaggcggt aaaacgcgaa gtggagcgtc gtgtgaagga
    16621 gcgcatgatt ctgtcacgta accgtaatta cagccggctg gccacagctt ccccctgaaa
    16681 gtgacctcct cagaataatc cggcacgcgc cggaggcttc cgcacgtctg aagcccgcca
    16741 gcgcacaaaa aatcagcacc acatacaaaa aacaatctca tgatccagct tctggtgcat
    16801 ccggtccccc ctgttttcga tacaaaacac gcctcactga cggggaattt tgcttatcca
    16861 cattaaactg caagggactt ccccataagg ttacaaccgt tcatgtcata aagcgccatc
    16921 cgccagcgtt acagggtgca atgtatcttt taaacacctg tttatatctc ctttaaacta
    16981 cttaattaca ttcatttaaa aagaaaacct gttcactgcc tgtcctgtgg acagacagat
    17041 atgtacctcc caccgcaagc ggcgggcccc taccggagcc actttagtta caacactcag
    17101 acacaaccac cagaaaaacc ccggtccagc gcagaactga aaccacaaag cccctccctc
    17161 ataactgaaa agcgccgccg tcccgcccaa agggcgggaa caacatcgct tttaattatg
    17221 aatgttgtaa ctatactccg tcatcgctgt cagtcttctc gctggaagta ttgagtacac
    17281 gctcgtaagc ggccctgacg gcccgctaac gcggagatac gccccgactt cgggtaaacc
    17341 ctcgtcggga ccactccgac cgcgcacaga agctctctca tggctgaaaa cgggtatggt
    17401 ctggcagggc aagggatggg taaggtgaaa tctatcaatc agtaccggct gacgccgggc
    17461 ttcggcggtt ttactccggt atcacatgta accacagcgt gccgccttcc atgccgctgg
    17521 cgcggcataa gcatatgttg gtggctgtgt ctgaaaggtt atatactcta cataaaacct
    17581 gacaacacgc agattgccag gcaaatgagc attatgcatc cggttatcgc ctgcctgtga
    17641 aacaatatca ccggggcgaa tatcgatact ctgactcgtt atacccatgc gtgtggcatc
    17701 aaaaaacctg caattgccct ttattgaatg aaagggggag tcaattatga atagcaccac
    17761 acattacgaa aacgccaatt tcctgcgcga gctggccgaa agcctgcctc gtatttttcc
    17821 agaaggcagt acagacaagt ctgcactatt gcaacgcctc gctaatgaag aactggcacg
    17881 ggcggaatac gacgaacaga tccgagcgaa agttgccgct gcccgcgccg ataagcggcc
    17941 gggaatgagt tccgcgcaac tgcgccagca actacaggga cgttatcagg aactgcgcaa
    18001 tgagttatga aatagaatgg cgaccaggtg cgattgagga tataacggcg ttgtttgaat
    18061 atctggtcga gaatgcatct ctttgggatg caaggaacgt gacagagcgt ctgatcactg
    18121 caaccgatcg cctggctgat taccccaggc tgtatgaaac ggatgaacgc tatggtgaag
    18181 gggttcgccg tattagctta cttggacagc atgtacttta tgaagtgaat gatgaggcca
    18241 gtcgtgtcaa tatcctggcg gttgtaggcc agcgtcagaa tccccgattc attctttgag
    18301 tgtccggctt ggaactccat cttcagagaa tcgatcaacg ttctttagct ccttccacta
    18361 acagccgacc tatctcaaac accgcagctg accgtcccga cttcaccgct tcggctttct
    18421 gtccctcaac aaaccagtta ttaccgtccg ttcggaccaa ctcaaacatc catgacatat
    18481 cgtaaacgtg ttccgggatc agcgaccctt ctccggccag aatacccgcc gttatggagt
    18541 ggttgccccc gtgcacaaag ccaatacgcc agggcaacca caggtcaaca tagtggttgt
    18601 ttttatcttg cttccacggc ttccccttat gggttccaat gcgtgaaacg ttatcgatat
    18661 agcgtggtag gctccaaggc cacggcagca ctatgtcgct tgccagcgac aacggataat
    18721 cagcgccgtt catctcacac tctttcatct tttcgtaaga ttgtatttgg gtaagtccct
    18781 taaagaagaa ctcctgcggg gtcatatccg ggcgggcatc tgttccctcc tcagctaccg
    18841 ccagcaagaa atcactctgt actggccgca cgatagcgcg aatgagatct ggtagcgcct
    18901 taacgggtag tttctctgcc acagaaagca gatgattgaa gcgttttttt tcattggcta
    18961 tagttgcttt atctttcttg tttcggttcc agaacatcac tgtctgtagc cctccttatc
    19021 gtgatcagga tgatggtttt cattctaatg gtgtagtagt ttcacgtcac gcgcacgtgc
    19081 atattcttcg aaagcttcgg cgttagccgg tcaaccaggg ggttgaggcc caaccgtacg
    19141 agatcgtacg ctaagttcat tttttgagca gaagaggtga gtaaagaaaa cgagggtttt
    19201 gtttactctt cccgtcaatg ccagtagcca tgcggtttca gggactttca gtgttaataa
    19261 actcggtaat caaatcaacg taaacctgcc taacaacgcc acgactttat tgacttaaaa
    19321 tcatatagtt agcttcttag cgtacgtttc cgctccattg ggcttcaaac cccacaccca
    19381 atgacatgag aaaagctgga tctgattctc aaaatgctgg gatacccatc gagatggaga
    19441 actcagtcaa tgttccattc tttctgaaat atgtgtgggg acactgatct gtcgggagcc
    19501 ggatgcggta gttccgcagg tccggttctg aggaggggcc cgtccgggca accggcgggt
    19561 ctactcacct catcccccta caaccactcc cattcgttta agtattttta aacctgcttc
    19621 ctgtggtatc tttatagata agatcctgtt tatctcagga tttatatcga gaccttctgt
    19681 cattgaaaaa tcacagtgag acaaatcaga atgtctgaac tctgtattat taaactccga
    19741 accagtaaaa tcagatttac gaaggtctgt ttcctcaaat aaacaatcac ggaagcgact
    19801 ggataaaaaa atagatttct gtaaccgtaa actaataaaa tcaacaaatc tcaaatcaca
    19861 atttacaaga gaaaaagtac agggaaacat aatgtctgct gcatttactc cttgtaataa
    19921 acaatctttg aattcacagt caataagttt aaaaaaatta agttttaaat tctttattga
    19981 gcagtttacg aagcgacatt tttcgaactc acaatcgcga aagttaaagt tgtcaagctg
    20041 tattcgttca aatatacaat tgtaataatt tacacctgat aattcacttt cactgaggtg
    20101 tcgttttgtg attctttttt ctattatatc catgtgtcag tctcctgttc ccataacttc
    20161 tttaataaat gccttttctg gagttgtttc attattggat tgaataaaat cactcatatc
    20221 agaataacga tttatactat tttttgaaag catatagaag gggattttat ctcttgttaa
    20281 ttcatcaaaa atatgagttg agaaaaatac agttttcccg tcagcggtga tcttatttat
    20341 cagttcccat aacatcattc tgtactgaat atcaatgcct acagttggtt catccagtaa
    20401 aaataaacgg gcatttttgc aaagcgttag catcaaagat atgataagcc aacgcttctc
    20461 tccataactt accgtgtaag tccttttacg tcttatttta tcatagcgaa tgaaaaaatt
    20521 atcattccat ttatgattaa gctctgtcca tgcctgtttt ttagttaact tattgaagca
    20581 gcaaatcaaa tcaagaacct cctcattcct tagtgtacct gaaagattta tgacttgagg
    20641 tagacttact aattctccac cagagaaatt tcgcttgagg gtcccttcat aattactgac
    20701 tccgcccctg ataaggttaa aaagagtggt cttcccggct ccattttctc caagcagacc
    20761 tatcaactct ccctgctcaa tcttaagcga cagattattg aaaacagggg gtaaatttac
    20821 tttatatgaa aagctcagag agttgatttc taaaagaggt atggtcattg agaactccag
    20881 actggctcag ttttgaaggt tagtgctgaa ataatactta tgattaacat tatactatag
    20941 aaaaatatcg tcatcagaga caactcctga tctgactgga gtattgtaga atagatggcg
    21001 ataggattaa cctgatttat ccagtaaacg atcccctcat tatattttaa tgatacaatt
    21061 cctgatacca tacagacagt tattaataca gaatagatag tgctggctgt ttggaatgtg
    21121 actctgaacg atgccatgaa agtgagtgaa accatcataa atgcatttat gtatatcgat
    21181 tttaaaataa tcattactat ttgataatct ggacttgcct ggaatcctgt tagtacgact
    21241 aaaatgaaaa aaaacacata caaaatagac atgataagtg aggctattaa ttgagaacga
    21301 ataaataata gtcgaccatc catattatgc acgaaggtag caataaaacc actttctctt
    21361 cttccgacca gatacagaca gtaattaaac agaacaacag aaaaagaaat gtagcctaca
    21421 aaccatgata tttgttttcc gagccatgct gtatcaccat aatgaagttc aatttcattg
    21481 aaatgaagga aaaagaacaa cactgtaggt gacataattg tccatattaa gctgaaaggg
    21541 gttctgattt gttccatcag aaaaattatc gccattttta atgtaaccat ttgttatcct
    21601 tatgggaatg gtaccatctt tgattctctg actttaatac cgtccccggt atggtaaatt
    21661 ttattattaa tgtttattgc acctgagtta ttcatatgaa ggaaaaaatc agggcttact
    21721 atttttgtgt accagacaag gctgttactt actctttccc tagcatagta aacaaaaata
    21781 ttatcagaaa tttgttgaag atagtttaat aaatttgtgt ctgacgtaat attttcctca
    21841 agcgttaact tgacatcatc agacagtagt accgtatttt cacacaaatc cgtaaacgac
    21901 tcgtacttgt tgcatgacat aaagtgacgc tggtaactat caataatgtc atcatcagta
    21961 taaccgccaa taagaaagtt gtgcaggcat atatacgatt gccacaattc cactacggat
    22021 ttacaaagtg cttttttcag actattagca taggataatc cggtactgta ttttattcga
    22081 ctgattttgt tttttgtgcc atacagggtt agtactgcgt gtccaggagc tccgggcagg
    22141 gtgatatcga aaatcctgat atctccttct gacctgagag ccagtaaaat ctcatctata
    22201 tgatttatgc ctgttactat ttcactggat atttcagtat tggcttttcc ctgtaaccag
    22261 taaagaagga gggactgtct ctccataaat tcacacaagg aaccttctat ggcatcgttc
    22321 aaactaccat gaaagctaca tccgcatgta tctctgtcag gataaaattt cagatcatct
    22381 gcagcggtta tattgtcgag tgcaattatg actgcaggta tttcttgttg ttcaaggcta
    22441 aacagattaa aggctctgac cgttttaaat ttatgggtta tgatttcatc gcgggtcagt
    22501 gatgagatct gaaaaaatgc ttcggtaaaa gcctttgcag cagatggagg tatcatctca
    22561 tataatgttt tctgaccacc ggtgactatc tcattaaaaa aatgacggcg ttcaaaatat
    22621 tcacctattg ccccctgaat tgacttcaga gtttcaccgg ccgcgctggc tggtgtaata
    22681 cgctcatagt cccatatctg agaaaacgtt ggcatatttc tgttcagttt tggactcatg
    22741 gccattgtgg ccggccatgc ggacataagg ttactgtaga catttatcat cgtttacatc
    22801 tccaaaaaac tgaacgatca gtggcgctac agtcctctga tccagattca ttgtttttgc
    22861 tacgtatgaa tcggtatatc ccgcccatac ccggtttttc agtcctattt gatctgcaac
    22921 caatactgcg ttctgataca tcgaacctgt ttccattaag gccatccggt atcctctgta
    22981 gcgatatttg aacaaagctt tttcaaaaat aatgcaatag acgagagcaa aatggggttt
    23041 accaagacgt tccgaatccc caccggacag gcttcggtag agtgactgag tattacatgt
    23101 agcaacaggt tccagtgcct gacttagcgg caggtagtga taaacattag ttcctgcctg
    23161 ccagttttct gtattttcag ataatctgca taaaaaaact tcaatcgggt acaatgcccc
    23221 cccagaagga taaggtcttc ttacagagcc aggatcaggt gagctgaatg cattgactaa
    23281 taacttttca atagtacgaa tggaaagttt atttttgtcg caaaagttta ttcctgatgg
    23341 gagcctttca cgactaaaat tatgtgatga ggatatgttt attacggagc tgaaacctgg
    23401 cctgaagaga acttctttat tttcaaattt ctccagatgg gcaagaggta ccgcggacac
    23461 gaaagttcgt tcagcaaatt cggggaggga agcaaagtta cctctgacat gaaaatcttt
    23521 aacaatggcc agaacttcag gatctgtgta atgcgttact tccactaaag agagttcgtg
    23581 ttttgacatg ttatctctcc agacagctgc aagcctgcca gtgaattacc ggctctttac
    23641 ataaaataca ggtgattaaa tcagcgctaa cagatgacat aaagttatcg acatgacttt
    23701 taaccagtga agtacctgtc aactcctgaa gtcgccgttt tattaaatga tagctgaatc
    23761 ctctttctga ttcgcctaaa gccaatgctg gtagtgtcat ttggtatttt ttcagtaact
    23821 gaagtaagtt tgcccaggac atttcgttac gtctgaagct tttttcttcc ctgctcagcc
    23881 atctctctat atgacaaaaa tggcagggag taccaagatc aggtgaatat actccatcta
    23941 tcctgaatga ctcctttagg tagtagcttt ggatgaaggc aacaccaggg ttactttcaa
    24001 attttgaata gattcttttt acaatgtctg aatgatagtt ttcgagatgt aaccagatta
    24061 gtgttgaatc gctcacatta atattattat cttttatatc aatgatatga gaaacaaaaa
    24121 caccatcaga tttccattgt tcccgggcat tactgaaaag atgtggaaca tcgcttatta
    24181 tgactgagtt tacccagaga ggtgatgttt tcgttttatt tatcacgcct gtggtaataa
    24241 gaaactcttt gcccttgtct ttatcaataa gcgattcgtc cagtagttta tcaaggaggt
    24301 attcagaaat aataccctgt ttggatcgta ggctttcgag tacttttttc attccctcat
    24361 gtgtaatgtc tactgaagaa atgtaggtaa taagcagggt ctttgtattt ctggaaatta
    24421 tctcaaacgg cagaatattg atcatatctt ttcctttttt aatatcaggg agcaccatgc
    24481 tccctgaacg gttaattcaa cgtatcagat atgtgaacca cttccgccgc tgccaccgtt
    24541 tccaccacta caaccgttgc tgcaaccacc acaaccgcca ccttgaccac cgcagctacc
    24601 gccgccgccg ccgccgccac caccaccaat gccaacacct aatggagact ggcgtgataa
    24661 tttaagagca tcaacggaca aaactacacc aaattcactc gcttttaatt ccataaggac
    24721 tcctttttta agggaaatta atgcttatga catctgaata gtatgttggc caacactagt
    24781 tacttgccgt aattaactat attttgcaat gataatttga actaaatttg tacgcctgtt
    24841 aaatatttga taataatatc agttggttat gtttatactg ttattgtttt tttacaaaaa
    24901 ttaatattgt gtttacattt tgctttcagt tgctgtcagg atgtcacact acccctttaa
    24961 gcccccactg gtctggcagg gccggggatg ggtaaggtga aacctgtcaa tcagtaccgg
    25021 cttacgccgg tatcatatgc aacaactgag tgccgccttc catatcatat catttgcccg
    25081 tttcccgccg tgcagatccg gtttccggct gaagtggatc cagacctgat ttgttgctga
    25141 gtatcggtaa aagagcggct tataatctat tctgaaacat ggcaagtgaa tctgggctgt
    25201 ataattccgg caagtgtcat ctgtatttaa aggtcgtgtg agtatgaaat ttatggaatg
    25261 tgctgtccgg gatgtgattt atgggaccaa tgtccgtatt gtcaaaccgg tcaacattta
    25321 tgaatgtgaa ctcagggaca atgtgtttgt cggccccttc gttgagatac agaaaggctg
    25381 tgtcattggt agtggaagcc ggatacagtc gcacacgttt atttgtgaga acgtaacact
    25441 gggggagaac tgcttcattg gtcataacgt gacgttcgcc aacgatctgt ttcgttcagg
    25501 agcaccagat ccgtcaccgg ataactggat cagcattacc ctgggggatt cggttactgt
    25561 tggcagtgga gccattattc tgtctccgta tatatgcagc ggagctgtta ttggtgcggg
    25621 ggcagttgta gtaaaaccga ttgagaacaa aggcatttat gcaggaaacc cggcaagact
    25681 gatcagaata ttatgaaaaa gaaaagggcc gttactggcc ctttttatca gaggtaggtc
    25741 tgtgaatacg gcaggtgtac agaaatcgac tgccggggat cgatttccag ggccgcccgg
    25801 tattctgcgt cagtgatatc accttttttc agtaaataac cgagagcatc gtttcccagc
    25861 cctgacagta tgcgtattct ggcgatccgg actggcaaat aacccatcaa aaacgttatt
    25921 gccagacatt gcgaactcac caattttgat tattggagcc actttttcat aagttccgtg
    25981 aaattatttc ataataacga ccctgtttat tttatgtaat gactggcaat aaaataactt
    26041 gattaagttc cctttatcca gcctgatagt ggataaagga aactcaatca taatttaatt
    26101 agtttattaa cttcatcgca ttcttttttg tctcctcatc acttttttgc tccgattgtg
    26161 aagaggactg aatgattgtt gaagaattta aatcggccat ctcttttgga aatagcgatc
    26221 tcaatatatt cacgatatct gaaaatgtgg tttctttcgt cagcagttca cataaaagga
    26281 aaggggccct gggatcttgt ctgtataagg ccagacctgc cgtcagtgca gcccggttaa
    26341 ggcggctgcg ttccccttgc gggatactgt ccagtgtatc acaaacaatt ttatctgttt
    26401 gatttacatc tggatttagc ttaaaggcaa tggttctgcg cttgtccatc attaattacc
    26461 tatgagatac ataccgttaa ctaaatcata ttgagagtta ttggttttaa aaaaacgttc
    26521 atcacgaatc tgtgtgtgtt tttttactgc atcgcatatt aattctgcgc caccacctat
    26581 aaccataaca tgagtataac cagaaaattc attgagtgta tttaatacac gttgctcaag
    26641 tttacgaagt gcttcattca ttgcttcggt gactattgat attttgttct catcattaat
    26701 tcgttgcttc agatagttat tatcttttct gtgaataatt atatcatcag caagatagct
    26761 acttcctttt gttctcgcaa gagaaagggc atcttttact gcagatgtga ccagagatac
    26821 accaagagat gagtctccgt atattttact gatccccgat aatttcccca ttacctgaga
    26881 aatatctaat gtggtgcccc cgagatctat aattaataaa gaatctaact catccagttc
    26941 ttgtagaact tcataacctg ccggtataga ttcaggcatg acttttacat cttttattgt
    27001 gaatgtatcc ccaccattta atgtaatttt tttccggaag tttgctttct tacgctcaat
    27061 attttccgta ttgggttggt tattcctgtc gtaatactct gtcagaggaa gtgtgcaaac
    27121 aatatccact tcgcttaccg gcagaccact ggtcagtaag gcgtggtgca ccgcaacgac
    27181 attaacgtcg ctgtattgcc atgcgatatt ggttgtgact acagcatccg ggctgattgg
    27241 atcaaatgaa tactgttcgc cgttcagtgt gtagttaaag acctttttat caccaaaaga
    27301 aactgcccac tcgcgtttga agctgttcgg gctaatgtgc tgtttaattg ttccgtcgct
    27361 ttcctgccac tgtagtttga tgtttgttga accgtcatca atgaatacca acatttataa
    27421 aactccttat ggtgtttttt tggtgttttt ttgggtgtgt tttgggcttt aaatgggttt
    27481 atttgtcaag tttaccccat tttaactatc aatcaatgat tatttgtctt gttttggtgt
    27541 tttgttgggc ttggtatggg ttgtttttgg gttttgtttg gcggataaca aaagatgtcg
    27601 ccgggctcct ctttttactg cgccggctga cgcggcgcgg caggaacgct gcctgtggtc
    27661 agtgtcccgc gtccggccgg gcacgggacg ggcgctttta ccgctcccgg ctggcgtcgg
    27721 tgacagtgta cgccagcccg tcgccctttt ctgatgagcg cccttcaagc cgctttcgcg
    27781 gcatagcctt gccgtcaaaa agacgacggc tgcggtattc cacggtcggc ctgacccgtt
    27841 accgggcgcg gtgaacagcc cacaggcagc ggggaacggg cgccacaggg gcgccctccc
    27901 gatgctcctg tgaggataat tcccatgcca aactggtgct cgaatcgtat gtatttttct
    27961 ggtgaaccgg cacagatcgc tgagattaaa cgactggcca gcggtgcagt cacaccgctt
    28021 tatcgccgcg ccacaaatga aggtattcag ctgtttctgg ccggaagtgc cggacttctg
    28081 cagatcactg aaaatatccg ctctgagcag tgccccggag tgacggctgc cggacgtggt
    28141 gctgtatcac tggaaaatat cgcgttcacc cgctggctga cacacctgca gaacggtgta
    28201 ctgctggatg agcaaaactg cctgatgctg catgaactct ggctgcagag cggtactgga
    28261 cagcgtcgct gggaagggtt accggatgat gtcagggaaa ctatcactgt gcatttcacc
    28321 gcaaaaagag gtgactggtg tgacatctgg ggtaacgagg atgtatcggt gtggtggaac
    28381 cgtctgtgtg acaacgtact gccggaaaaa accatgccgt ttgacctgct gacggttttg
    28441 ccgacccgcc tggatattga agtgaatggc tttaacggcg gtgttctgaa cggtgttcct
    28501 tctgcatatc actggtatac agaacggtat ggcgtgaagt ggccttgtgg gtatgacctg
    28561 aatatcagca gtcaggggga aaacttcatt caggtggatt tcgacacgcc gtggtgtcag
    28621 ccggaaagcg acgttgttgc agaattaagc cgccgtttcg gctgcacgct ggagcactgg
    28681 tacgccgaac agggctgcaa tttctgtggc tggcagttgt atgagcgcgg agagcttgtt
    28741 gatgtgctgt ggggggaact ggaatggtct tccccgacag atgacgatga gctgccggaa
    28801 gtcaccggac ctgcgtggat agtcgacaat gtgacgcatt atggcggatg aagtatgacg
    28861 gagacgggcg ggcaaccgcc cgtttctttt ccgacaaagg atgtcgccgt gctcctcttt
    28921 ttactgcgcc ggctgacgcg gcgcggcata aacgctgcct gtggtcagtg tcccgcgtcc
    28981 ggcgggcacg gggcgggcgc ttttaccgct cccggctggc gtcggtgaaa gtgtacgcca
    29041 gcccgtcgcc ctttactgat gaacgccctt caagccgctt tcgcggcata gccttgccgt
    29101 caaaaagacg acggctgcgg tattccacgg tcggcctgac ccgttaccgg acgcggtgaa
    29161 cagcccacag gcagcgggga acgggcacca caggggtgcc ctcccggtgt cctctgtaag
    29221 agaaggagtg ctgttatgtc ccgttttatc ctgggtgact gcgtgcgtgt tatggccacg
    29281 tttccgggca atgctgtcga tttcatcctc accgacccgc cgtatctcgt cggtttccgt
    29341 gaccgttccg ggcgcaccat cgccggcgat aaaaccgatg aatggctgca accggcctgt
    29401 aatgaaatgt accgcgtgct gaaaaaagat gcgctgatgg tcagcttcta cggctggaac
    29461 cgcgtcgatc gctttatggc cgcctggaaa aatgcgggat tcagcgtggt tggccacctg
    29521 gtgtttacca aaacctacac atcgaaggcc gcatatgtgg gctatcgcca cgaatgcgcc
    29581 tacatcctgg caaaaggacg tccggcgctg cctcagaagc cattgcccga cgtgctgggc
    29641 tggaaatatt caggcaatcg tcatcacccg actgaaaagc ccgttaccag cctgcaaccg
    29701 ctcattgaga gcttcacaca cccgaacgcg attgtgctgg acccgtttgc aggcagcggc
    29761 tcaacctgtg tggccgccct ccagtccgga cgccggtata tcggtgtcga gctgcttgaa
    29821 cagtatcacc gtgccggaca gcaacgactg gccgccgtgc aacgggccat gcagcagggg
    29881 gccgcgaatg ataactggtt tgaaccggag gctgcgtaaa tgaactatgc cggacacgaa
    29941 aaacttcgcg ccgacgtggc ggaggtggcc aacaccatgt gtgaccttcg ggccaggctg
    30001 aacgatatgg agcatcgctg tcgctttgat tccgatgtgc ttgttgaacg cctgacacgc
    30061 cagacgctgt atcgcgccaa tcgcctgttc atggaggcgt ataccgaaat tctggaactg
    30121 gattcctgct tcaaagattg agaaggagac gagaatgtac ggaacatgcg aaacgctatg
    30181 ccgggagctg gcagcaaagt atccgggaga cacgccgctg atgctggtta tctggtcccc
    30241 ggaagaaatt caggccctcg ctgacggaat ggatatttcc ctgtccgatc atgaaatcag
    30301 gactgtcctg gcgcgcctgg aggacatccc ggaagaccag cggactgaat ccggtatttc
    30361 ttccggtgcg gcgatggaga tcatcaataa cgtgagagaa aaccgccagg taaccgtccc
    30421 tgctgaactg ctggcgtccc tgattcagac cgctgaacag gcattgtgga aacgtgaatg
    30481 ggccgcccgg gatcatggcc tcgccgtccc ggaatgcgtc acccgccgtc aggcggtggt
    30541 taatcaggtc cgcatcctgc tgaaaaacaa cacacacgaa aacgactgat gttatcgccg
    30601 cctccgggcg gcgatacagg gagttcatga attatgaatg aaacgctgaa tgcactgatt
    30661 tatcgccacg ccagcaacct gttgctggcg cagggctggc cggaagagac ggatgttgac
    30721 cagcgtaacc cgaagtatcc gggctggatc agcatttacg tgcggctgga tgcgccccgg
    30781 ctggcgacgt tacttatcaa ccgtcacggc ggcgtactgc cgccgctcct ggcctctgcc
    30841 attcagaaac tgaccggaac cggagcggaa cttgttctgt ccggcagtca gtggcagtcg
    30901 ctgccggtac ttccggcaga cggaacgcag gtgtctttcc cgtatgccgg tgagtggctg
    30961 gcagaggacg aaatcagggc cgttcttggt gcggtacgcg atgcagtatg cagtgtcagt
    31021 taccaggtgg cagaagatgc gcggcgtatc cgtgcggcgc tgaccaccac cggtcagacg
    31081 ttgctgaccc gccagacgcg ccgctttcgc ctggtggtga aggaaagcga tcacccctgc
    31141 tggctcgatg aagacgacga aaacctgccc gtggtgctcg atgccatcct gaaccggggc
    31201 gctcgttttt cgtcggtgga aatgtatctg gtcagtgagt gtattgagca tatcctgtcc
    31261 agtgggctgg cctgcgatgt gctgcgtata ccggatgaac cgccccgccg ctggtttgac
    31321 cgtggtgttc tgcgggaggt ggtccgggaa gcccggaccg aaatccgcag catggcggat
    31381 gccctggcaa aaatccggaa atgatgacac tggcggggaa aaatctccgc cttttccagc
    31441 cggtccccgg aaagagcaaa aggatgtcgc cgcaggctcc gctttacccg gccatgcggg
    31501 gcatggcctt gtgggttttc agttatgtgg cctcagcgtc gtgtgcgggc tgtgccgtgc
    31561 ctccatcgta gccgggcttc tgcgggcggg ttgcaaggta aggtgaaccg ctgcggtcac
    31621 ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cacccgctca gatttcgcgg
    31681 tctcaccctc caactcccgt cagccgtgcg cgtcaggctg cggcttccct tgcgcccttg
    31741 caaccctgca ggcccggcaa agatggaggc acggcaccgc ccggtgtcgc ggaatatgtg
    31801 aacaacggag gaaaacatca tgcaatatgc gaaacctgtc actctgaatg ttgaagagtg
    31861 cgaccgtctc tcctttctgc cttacctgtt tggcctggat tttctgtatg ccgaagcgtc
    31921 tgtatacgcg ctggcgaaaa aaatgatgcc ggaatatgaa ggcggattct ggcacttcat
    31981 ccgcctgccg gacggtggcg gttacatgat gccggatggc gaccgtttcc acatggtgaa
    32041 cggtgcaaac tggtttgacc gtacggtcag cgccgatgcc gcaggcatca ttctgacttc
    32101 ccttgtgatt aaccgccagt tgtggctgta ccacgacagc ggggatgcag gactgaccca
    32161 tctttaccgg atgcgcgatg cgcagttgtg gcgtcacatc gaatttcacc ccgaatgcaa
    32221 cgcgatttac gcggcactgg actgattaac ggacggggcg gcaatgccgc ccctgtaaag
    32281 gagacgagaa catgtactgt actgttaaag aaatcatccg cgatgtactg gatacagacg
    32341 tgccggacag tgaatgcgtt tttgccgtgg tgctgacccg tggggatgtg cgctacatag
    32401 cccaggactg gaacctgtca gacgatgagc tggaaaccgt catgcagcgg ctggacgatg
    32461 cctttgaata tggtgcggat gtcagcattg ttcacgacgt tgttcgtgag ctgatggaag
    32521 aaaagcgagc cagccgtcag gtgacagttc cggcggtgat gctggaaaaa gtgatggcgc
    32581 tggcaggcag tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg ggcgacggtg
    32641 acgcattcgt cagggaagaa cgcgaagcaa tggacgttgt gttacaggcg ctggacgggg
    32701 agcacatgtc atgaatatca gcacagaaac ccgcgaaatt ctgcgtaatt acagggccgt
    32761 gattaatgcg cggcgtcgtg aaatggggca gaaaccgctc accaccgcgc agattgttga
    32821 tgaaatctgc gattttgtgg cgaatcagca ggcggttttc ctgggcggtc actacatctt
    32881 acagggtagc agaaacaggt gatacagggg caggcggtgg aggccgcctg tcagtccgcg
    32941 cgaaaacccc tggcaggctg acgcctgccg gaagggaagc gggccacgct ggcgcgtcgc
    33001 ccgttaccct tcccgtctgt gatgtgtctg actcaggagt gtttctttct tatgttatgg
    33061 aactgttcct gatgttgttg ttcctttatc tgctgctctg attctgacta ctgcagtgtt
    33121 gttctgtctg ccgggcgcac cttcggctcc cggctgcctg gcggcatccg gtcgtcaggc
    33181 ccgactccga caggcagctg ctggcgcacc cgccagtctc cgccgaaccc gctggcgcgg
    33241 tttcgggctg tcgctacggt ccgatgcgtg aaaaccccgg gcttgcagcg accgttccgg
    33301 tcgcgccacc cttgtcgcgc cgtggcctgc gctcccggca tcccggcaaa aggccgccgg
    33361 gatgtaaggg gcaacgccgt ttcgcgcacg cgctctccgt cgttgcggcg ggtccctgct
    33421 cattgcctgc cggtgccaca cacccgtatg agtccctgtc gcctggggct gatacagcca
    33481 caacctgccg cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa
    33541 cccggtaaaa aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc
    33601 ccttgcggcg aaggttctca aacgggtgaa gttcatacca cagggtatac agcagcaggt
    33661 tcccgcgaaa cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc tttaaaaatc
    33721 aggatttatt tcaggctccg gcaatacgca tttatgtgtt ctgtcagtca tccggctggc
    33781 aggtaatgag aacgcaggaa cagaaggagg aaaacaccgg agaacgtcag acgcagatat
    33841 gaaaaaagca ggtcagggac ctgcttaatc cacaccgcag agactgcacg aacagtcttt
    33901 acggtttcat taattaacgt cataagaggc taaattatga cagtaagtat tgtttcacct
    33961 tccgcagcgg ctgtaaagcc ccgccgtcat ccccgtttca gacgcgaaga catccccgcg
    34021 ccggaaattg accccgtgct gaaagccttc gggcgacaca ttgcccggag ttttcaccgg
    34081 gggcgtggtg tacacatccc cgccatgaaa aacacggcct tcgggcaggt gctccgcaca
    34141 ctcgaactga aacgcgcttt taactgatga ggtgagccgc cccgcgaggg gcggcaacag
    34201 acggaaggag aacattttat gacattagca gcaatcacca tgaccgcccc ggaagccgcc
    34261 agccctgtgc agatgtaccg cgcgacctac tcaccggatg acaacaaact gcgcctgtat
    34321 gccgtgtcac gtcttgaccc ggagacgtat aaaaaagtgc atgatgccgg tttccgctgg
    34381 gcaccaaaac aggcgctgtt tgtcgcgcca gcctggacac cgggccggga agacgtgctc
    34441 ctctcacttg ccggagagat tgaggatgaa gacagcacgc tcgctgaacg tcaggaagca
    34501 cgggcggagc ggtttaccgg atacagcgga aagcgggcca gtgaatccgc acaggcactt
    34561 gatgaagtgg aaagactggc cgcgatgatc ccgcccggtc agcccattct tgtggggcat
    34621 cacagcgaac gccgcgcccg tcgtgatgcg cagcgtattg aaaacggcat gaaacgagcc
    34681 gtgatgctct ttgaacgtgc ggaatactgg gaagagcggg cgcggtcggc actgcttcac
    34741 gcgaagtata aagaacgtcc ggacgttcgc tggcgtcgta tcaaaaaaat cgaagctgat
    34801 ttgcgcaagg ctgaaaagac catcgcgcag tcgcagaaat atctgacgat gtggcgggct
    34861 gaatcgctgg atctgaatat ggcaaaactc atcagcagtc atgaccatat cagcgcctgt
    34921 ttcccgctgg atacgtatcc gcgcccggca gaaaaaagcc agtatgaagg aagtcgatcg
    34981 ttatggtcgg ccctggatga tgacatcatc accacggagc aggcccgcga aattgcgatc
    35041 cgctgtcatg aacggcagat tcagcatcaa caacgctggg ttaaccacta tcagaaccgc
    35101 ctgatctatg agcgtgccat gctggacgaa agcggcggcg tggttacccg gacacaggat
    35161 tttgagccgg gcggacaggt tttcagccgg ggcgagtggc tgaccatcat ccgtgtgaac
    35221 aaaagcaacg gggcggtgag ttcagtcaca acgccaaatt acagttttct cgggtacagc
    35281 ggcacgatga aagtgacgcc cgatcgcatc acggactaca aagcaccatc ggcagaagag
    35341 gctgccgtcg ccagtcaggc cgcgaagcgt ccgccggtag tcaactatcc gggggaaggt
    35401 ttccgggaaa tgacaaaggc acagtgggcc gccctgcccc gggactgtaa ggccgtgcgc
    35461 agtgtggcag aagcagaaga ccacggggca taccggtacc gccgcacaat ggacaataat
    35521 ttccgcctgg tgaatgtgta tatctccgac atgaaaatca cggaaatccc acagaaataa
    35581 ggtatatccc cccgggaaaa tccccggggg atgacggaga taatgatatg cacagtcagt
    35641 taagagaacg tatccggctg atgcgcgcaa ggctggataa cgccgcgccg gttgctgaaa
    35701 tccgggctga atctcagctt tttgtgactc ctgccccggt ctgtgatcgc ctggtgatgc
    35761 tggcggagat cagcaaccgt gatcacattc tggaaccctc tgccggcacc ggggccatac
    35821 tgcgggcgat tcgggatacc gcgccggggg ccatgtgtga tgcggtggaa atcaacagcg
    35881 ggctggtccg gtatctgcgg gaaaatttta acggtgtcag ggttcagtgt ggcgacttca
    35941 tggaatggct gccggtgcag tattacagcc gggttatcat gaatccgccg ttcagccacg
    36001 ggcaggatat ccggcatatc ctgcgggcct tttccctgtt gcgtccgggt ggcgtgctgg
    36061 tcgccgtctg tctcaacgga ccacgccagc aggagaagct gttaccgttt tctgacgtcc
    36121 gcgaggagct gccgcgcggc acgtttgctt ataccgatgt cccgacgatg attattcgtc
    36181 tgcgtgcctg atgacacagg gccggcggga ggataaatgc ctgtctgccc gcgccgggaa
    36241 aaaacgcgcg ccggtggcgc tgaaaggggc cgcgctcacg cgccgcccct ttcattacgc
    36301 ggacgttctg atgggggctt ctgtttcttc atgcctgtca tttgtctgtt actgatgttg
    36361 ttgttcctgc tactgctact gctactgcta ctgctactgc tactgctact gctactgcta
    36421 ctgctactgt tcttcttttt cctgctgctc tgattctgac tactgcagtg ttgtcctgtc
    36481 tgccgggcgc accttcggct cccggctgcc tggcggcatc cggtcgtcag gcccgactcc
    36541 gacaggcagc tgctggcgca cctgccagtc tccgccgaac ccgctggcgc ggtttcgggc
    36601 tgtcgctacg gtccgatgcg tgaaaacccc tggcttgcag cgaccgttcc ggtcgcgccg
    36661 cctctgtcgc gccgtggcct gcgctcctgg catcccggca aaagaccgcc gggatgtaag
    36721 gggcaacgcc gtttcgcaaa ccgcgcttca cgtcgttgcg gcgggtccct gcccgttgtt
    36781 ctgtccggtg ccattcacct gtttaagccc ctgtcgccgg gggcaaagac agccacaacc
    36841 gtctccgcca gtccaggggt gaaatgcaca tcccgcaaaa ctttttgccc agaagcaggc
    36901 aaaaactttt gcggtctgcc cccggacagt cgtttccgtc cgtggcagtg atgccccatg
    36961 cggcgaaggt tctcaaaaag gtgatggtga ataccggagg gaacaaaagg caggtccccg
    37021 caaaacgtca aaaccgcggc tcccgaaact caaaagatga tgggggctga aaacatcaac
    37081 agaaggaggc acatcatggc agttcgtggc attaacaagg tcattctcgt aggacgcctg
    37141 ggaaaagatc cggaagtccg ttacatcccc aacgggggcg cagtggcaaa cctgcaggtc
    37201 gccacgtcgg aaacctggcg tgacaaacag acaggggaga tgcgggagca gacagaatgg
    37261 catcgcgtgg tgctgttcgg caagctcgcg gaagtggctg gcgaatatct gcgcaagggc
    37321 gcgcaggtct acatcgaagg tcagcttcgc acccgtagct gggaagataa cggtatcacc
    37381 cgttacgtca ccgaaattct tgttaagacc acaggcacca tgcagatgct gggacgtgcc
    37441 gcaggtgctc agactcagcc ggaagagggg caacagttca gcggtcagcc tcagccggaa
    37501 ccacagtcgg aggccggtac gaaaaaaggt ggcgcaaaaa cgaaaggccg tggacgtaag
    37561 gccgcgcagc ccgagcctca gccgcaaccg ccggaggggg aggattacgg gttttcagac
    37621 gatatcccgt tctgatcggg ctgactgtga taaccgcccc gccccgttct gtgcggggca
    37681 tcaccggaga gatgagaatg agcgaatatt tcagaatact tcaggggctg cctgacggtc
    37741 cttttacccg caaacatgcc gaagctgttg ccgcacagta ccggaacgtc tttatcgagg
    37801 atgaccacgg cgaacagttt cgcctggttg tccgtaataa cggtgcaatg gtctggcgta
    37861 cctggaattt tgaggacggt gccgggtact ggatgaacca tgtcatcagg gatttcggga
    37921 ttcttaagta agagaggtgc cggacgggga cacgtccggc agacaatcat tattaccgtt
    37981 ttgggaggta ctaactatgt cagttacaga gtctaaggca aaaacggagc gtaaatccag
    38041 ccgtaaacct gcaaaaacgc aggaaacagt cctgtctgcc ctgctggcgc agacggagga
    38101 agtgagcgtg ccgctggcct cactgattaa atcaccgctg aatgtgcgca cggtgccgta
    38161 ttctgtggag tccgtcagcg aactggctga gtccattaag ggcgttggcc tgttgcagaa
    38221 tctggtcgtg cataccctgc ctggtgaccg ttacggtgtc gccgcaggtg gtcgccgact
    38281 ggctgcactc aacatgctgg cagagcgcaa catccttccg gctgactggc ctgtacgcgt
    38341 gaaggttatt ctgcaggaac tggcgactgc cgcatcaatg actgaaaacg gtcatcgtcg
    38401 ggatatgcac cctgccgaac agattgccgg attccgcgca atggcgcagg aaggcaaaac
    38461 acctgcgcag actggcgacc tgctgggcta ttcaccccgc cacgtccagc gaatgctgaa
    38521 actggcagac cttgcacccg tcatccttga tgcgctggca gaagaccgca tcaccaccga
    38581 acactgtcag gcgctggcgc tggagaacga caccgcgcgt caggtgcagg tgtttgaagc
    38641 cgcctgccag tcaggatggg gcggtaaacc ggatgtgcgg gttatccgca acctgattac
    38701 cgaaagtgaa gtggcggtgg ctgggaacag taaattccgc ttcgtggggg ctgatgcctt
    38761 ctcgccagac gaactgcgca ccgatttgtt cagcgacgac gagggtggct atgtggactg
    38821 cgtggcgctc gatgccgccc tgctggaaaa actccaggct gtcgcagagt tccttcggga
    38881 agccgaaggc tggggatggt gtgccggacg catggagcct gtaggtgagt gccgtgagga
    38941 tgccggaaca taccgctgtc tgccggagcc ggaagcggtg ctgacggagg cggaagaaga
    39001 acgtctgaac gaactgatga cgcgttacga cgcgctggaa aaccagtgtg aggaatctga
    39061 cctgctggaa gcagaaatga agctgattga ctgcatggcg aaggtcagag cgtggacgcc
    39121 ggagatgcgt gccggaggtg gagtggtggt gtcctggcgt tatggcaacg tgtgtgtcca
    39181 gcgaggtgtg cagttgcgca gtgaggatga cgtggctgac gacgctgacc gcacggaaca
    39241 ggtgcaggag aaagcgtcag tggggggaat cagtctgcca ctgctgacga aaatgtcttc
    39301 cgaacgcacg ctggcagtcc aggcggcgct tatgcagcaa ccggacaaat ccctgacact
    39361 gctggcatgg acgctctgcc tgaatgtgtt tggcagcgga gcgtacagta aaccggcaca
    39421 aatcagcctg gaatgtaaac attattcgct gaccagcgat gcgccgtcag ggaaggaagg
    39481 tgtcgcattc atggcgctga tggcagaaaa agcccgtctt gctgccctgt taccggaggg
    39541 atggtcacga gacatgacga cattcctgtc actcagtcag gaggtgctgt tgtccctgct
    39601 cagtttctgc accgcatgca gccttaacgg tgtccagacc cgtgagtgtg gtcacacgtc
    39661 acgcagtccg cttgactcgc tggaaaccgc tatcggattc cacatgcgcg actggtggca
    39721 gccgacaaaa gcaaacttct tcggacacct gaaaaagccg cagattatcg cagccctgaa
    39781 tgaggcagga ctgtccggtg ctgcacggga tgcagagaag atgaagaaag gtgatgcggc
    39841 tgaacatgca gagcaccata tgaaagacaa ctgctgggtg cctggctgga tgtgtgcacc
    39901 acatccacag acggatacca ctgaacgcac cgataacctg gctgatgccg cctgataaac
    39961 aaccacaccg ccccgccgga gacggggcgg cagcaaggga gataccgtga tgaaaactga
    40021 actgaccctg aatgtattac agaccatgag cgcacaggaa tatgaagata tccgggcagc
    40081 cggaagtgat gaacgtcgtg agctgacaca cgccgtgatg cgggaactgg atgcgccgga
    40141 caactggatg atgaatggcg agtatggcag tgagttcggg ggctttttcc ccgtccaggt
    40201 tcgtttcacg ccagcccacg aacgtttcca cctggcgtta tgttcaccgg gcgatgtctc
    40261 tcaggtctgg gtgctggttc tggtgaatgc cggtggtgag ccgtttgcgg tggttcaggt
    40321 acagcggcgg tttgcaccgg aagccgtcag ccattcactg gcactggcgg cgtcactgga
    40381 cgcgcagggg tacagcgtca gtgacatcat ccatatcctg atggcggaag gaggtcaggc
    40441 atgagcgcac gttcacaggc actggttcct ctcagcacag agcaacaggc cgcatggcgg
    40501 gcggtggcgg agaccgaaaa acgccgccat cagggtaaca cgctggctga atatccgtat
    40561 gcgggggcgt ttttccgctg tctgaacgga agccgcagga tatcgctgtc tgacctgcga
    40621 tttttcatgc cgtccctgac cgcagaagaa ctgcacggca accgtcttca gtggctgtat
    40681 gccatcgatg tgctgattga aacacagggc gaggtctgcc tccttcccct tcccggtgat
    40741 gcagcagagc gcttgttccc gtcggtgcgg tttcgtgtca gggaacgcag tcgccataaa
    40801 tccgcgctgg tcatgcagaa gtacagccgc cagcaggcga gggaggcaga gcagaaagcc
    40861 cgtgcgtacc aggcgctggt cgctcaggct gaaatcgaac tggcattcca ttcgccggaa
    40921 acagtcggaa gctggcatgc acgctggagt gaccgggtgg cagagcatga cctggaaacc
    40981 ctgttctggc agtggggcga gcgttttccg tcactggccg gaatggaacg ctggcagtgg
    41041 caggatatgc cattctggca ggtcatcgca gaggctggta tggcggcaag ggtggtcggt
    41101 catgctgttc gtgagatgga gcggtggatg gtgccgaaca aactccggga ggcagcgtga
    41161 tgcggcaaca atcacacgga tttcccgtga acggtctgaa tgagcggatt attttcaggg
    41221 aaagtgagtg tggtcagcgt gcaggtatat gggctatgat gtgcccggcg cttgaggctt
    41281 tctgcctcat gacgtgaagg tggtttgttg ccgtgttgtg tggcagaaag aagatagccc
    41341 cgtagtaagt taattttcat taaccaccac gaggcatccc tatgtctagt ccacatcagg
    41401 atagcctctt accgcgcttt gcgcaaggag aagaaggcca tgaaactacc acgaagttcc
    41461 cttgtctggt gtgtgttgat cgtgtgtctc acactgttga tattcactta tctgacacga
    41521 aaatcgctgt gcgagattcg ttacagagac ggacacaggg aggtggcggc tttcatggct
    41581 tacgaatccg gtaagtagca acctggaggc gggcgcaggc ccgccttttc aggactgatg
    41641 ctggtctgac tactgaagcg cctttataaa ggggctgctg gttcgccggt agcccctttc
    41701 tccttgctga tgttgtacgg gcatgaacaa cctgacttca ggaaggtcgt ttcccttcag
    41761 gaacggggat gaacgcgcgc ctgcggcgcc cgttcttttc ccccgccttc tctggttatg
    41821 gcctgtcaga aatcacatca ccttccctgt gattattctc tttttcttcc tgctctgatt
    41881 ctgactactg cagtgttgtc ctgtctgccg ggcgcacctt cggctcccgg ctgcctggcg
    41941 gcatccggtc gtcaggcccg actccgacag gcagctgctg gcgcacctgc cagtctccgc
    42001 cgaacccgct ggcgcggttt cgggctgtcg ctacggtccg atgcgtgaaa accccgggct
    42061 tgcagcgacc gttccggtcg cgccgcctct gtcgcgccgt ggcctgcgct cctggcatcc
    42121 cggcaaaaga ccgccgggat gtaaggggca acgccgtttc gcaaaccgcg cttcacgtcg
    42181 ttgcggcggg tccctgcccg ttgttctgtc cggtgccatt cacctgttta agcccctgtc
    42241 gccgggggca aagacagcca caaccgtctc cgccagtcca ggggtgaaat gtacatcccg
    42301 caaaactttt tgcccagaag caggcaaaaa cttttgcggt ctgcccccgg acagtcgttt
    42361 ccgtccgtgg cagtgatgcc ccttgcggcg aaggttctca aaaaggtgat ggtgaatacc
    42421 ggagggaaca aaaggcaggt ccccgcaaaa cgtcaaaacc gcggctcccg aaactcaaca
    42481 gatgatgggg gctgaaaacc agagagagga gaaagcagga tgagtacccg aaatatccac
    42541 gttaacaccg cgtcgtacac gcttcttgtt gccgggaaga aaaaaaacac cggggaagag
    42601 tgggacgtcc tggaattcag cagcctgact gagctgaaaa aatatcgcaa aagccacccg
    42661 gaaaagatgg ccttcagtta tagctacgcg ctcagtcagg gtgtggataa gcagttccgc
    42721 catatcaaca ttgcggaagc cgatcacttc aaacagttcc tgcgtcagat taagcgtgcc
    42781 ggcctcgata tccgggcgat ctgctgaccg gatgtgatga atacccctcc tgattgttgc
    42841 aatcttgtta atggagatcg ggggggatga tattaagcgg aaaaattttt tattaaattt
    42901 attctttcat aaaggagtag ctgttatgcg attagcttcc cgttttggtc ggtataattc
    42961 catccgccgt gaacgtcctt taacggatga tgaattaatg cagttcgtgc cttcggtatt
    43021 ttccggtgat aaacatgagt cccggagtga acgttatacg tatattccaa caatcaatat
    43081 catcaataag ttacgtgatg aaggtttcca gccattcttt gcctgtcaga gtcgggttcg
    43141 tgatttggga cgtcgcgaat acagtaaaca tatgttacgt cttcgcaggg aagggcatat
    43201 taacggacag gaagttcctg aaattatcct gcttaattca catgatggtt catccagtta
    43261 tcagatgatc cccggaattt ttcgttttgt ctgcacaaat ggcctggtgt gcggaaataa
    43321 ttttggcgaa atccgcgttc cacataaagg tgatattgtc gggcaggtta tcgagggagc
    43381 gtatgaagtg ctcggtgtct ttgataaggt cactgataat atggaggcga tgaaagaaat
    43441 tcatcttaac agtgacgagc aacatttatt tggcagagct gcactgatgg tcaggtatga
    43501 agatgaaaat aaaacgccag tgacacctga acagataatt actccccgtc gtcgggaaga
    43561 caaacagaac gatctctgga caacctggca gcgggttcag gagaatatga taaaaggtgg
    43621 attatcgggg cgaagtgcct ccgggaaaaa taccaggaca agagccatta caggtattga
    43681 tggtgatata agaatcaaca aggcgttatg ggtgattgcc gaacagttca gaaagtggaa
    43741 gtcatgataa tataagtaaa gccccgaaaa aattttcggg gctttactta tattatcatt
    43801 tttcggcatt gctgtgtcgt ctgatatacc tcaatttcat caaccggctt tccggttgtc
    43861 tgtaaatatt gtgcctgcga aaaatggtct ggcaggaggc tgcgcagtta ccgggtatat
    43921 ataagatggg ttcccgcttc gcggggcttc ggccagtcag tattgtcact catatgatat
    43981 ttttgtgtgt ggccttccat gccgcttttg cggcataacc ggtatctgac aatgtctgta
    44041 agattaattg ttctgcacgc tgttaatttc cggaagtgat ttcttcgtgg ccggaagccg
    44101 gataccttta ctgcttttta tcccggtata aatccggtaa acatctgatg cgtaggcaag
    44161 acgtctctgg ttctggcgtt cggttttcct gaatccggca ttgtatgcac caacggcctc
    44221 ccaggtgacg ccccattttt taaatgctat tgccagataa taagcaccgg tataaatgtt
    44281 catgcaggga tctgttgtca gatgttctgg cttaattccg tagcgggcca gttcgttaaa
    44341 atgctgggaa tctacctgca tcagtccgct gccatatccc gttaccggat taataccgat
    44401 ggcattaacc cggtaacggg attcttgcca tgatattgct ctcagtaaat caggatctat
    44461 tttgtaatcc cggcctgcaa gatcaaagca atcagtggct tggcagatct catttataaa
    44521 catcaggcag atggctaaca tccatttttt catttttcca cctctggtga ctttatccgt
    44581 aaataattta acccactcca caaaaaggct caacaggttg gtggttctca ccaccaaaag
    44641 caccacacac tacgcaaaaa caagtttttg ctgatttgct acttgaatca ttaacttatg
    44701 ttttaaataa tgtattttaa tttattttac attataaaaa ggattcattg gtgaatcgca
    44761 tatgattcac caatgaattt aacacaactc atagatgaat cagtaataat aaaacaacga
    44821 gttaaagcga attgactcta gattcaattc gtgatgtgat ttgctgcatg aatcgtaatt
    44881 ttcgtaactt gcgtcacgat tcgattcatc gatgaattgt ctttttttta tatgtgaaat
    44941 aaatttgatg cgataagaaa ataaagaggt atacatatgg cgaaagtgca ggcttatgtc
    45001 agtgacgaaa ttgtctataa aataaataaa attgtggaaa gaagacgtgc agaaggagct
    45061 aaaagtacag atgtaagttt ctcaagcata tccactatgc ttctcgaatt agggcttcgt
    45121 gtatatgaga ctcagatgga gcgtaaagag tctgcattta atcagactga gtttaataaa
    45181 ttgcttcttg aatgcgttgt aaaaacacaa tcatcagtag cgaaaatttt gggtattgag
    45241 tctctcagtc ctcatgtctc cggaaatcca aagtttgaat atgcgaatat ggtcgaagat
    45301 atcagggaga aggtgtcatc tgagatggag aggttctttc cagaaaatga tgagggttaa
    45361 aaaagaatta aaagttgatt tgcttcaaat aacaggattt tgatctggtt caatttgatg
    45421 acaattaacc gcataaaggt tatattaatt atgtggttaa tgccacgtta aaacttgata
    45481 ttaaaaatcg ccgatgagag aaaggccact taacagtcaa tctgtaaata aatacattct
    45541 taacgttcaa aatatctaca ggaattctcc tgttcctgtt tgtgtccgta acaaaaaaag
    45601 gaaaatcctt tatgccaatg gggcctttat tgaactcttt tccaaagaag ataaaccctt
    45661 ctctggtgaa agttatgtac gtctgcaggt tgaaattttt ctttcatcac ttgagcttga
    45721 atgccagtct cttgggcatg gttccgcatt ttgtcgtcgt tttaattttc atggagaaat
    45781 ctatcaaata aggatggaaa atgtttcatt ttataatgaa gaatctgttg ttctatggca
    45841 aattaatata tttcctgact atccattctt tcgtgtggaa aaagaaaatt attatcatcg
    45901 tgatagctat gtacagtctg ttatatcgaa tatgacagca aaatcattgg ttgttttttg
    45961 tttctatgca ttagggtata aacatataaa tatagctaaa gaattaaaaa tcacagaagt
    46021 ggcttctaaa aaaaggatta aaaagattaa tgcggatatc aataaaagat tgggttcctt
    46081 cgatttgttc aggaataaat gtattaagac aggtgtaatg tacgaaatat taaatgtttt
    46141 gagtgaatat atgggtgtta aaaaaatgta ataaagcagg gctcgttttg ggcccatttt
    46201 cgacaggcca tttctgtgca atcatgattt tattggtcag gaaacagagg tgaattgagt
    46261 gaggaggcgt aacgcgagag gcgggataag cagaacagtc tcagtgtatc tcgatgaaga
    46321 tacaaataac cgacttattc gggccaaaga tcgtagcggt agaagtaggt aatggtgcca
    46381 actta
//
LOCUS       DAEEDS010000028        46143 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEEDS010000028 DAEEDS010000000
VERSION     DAEEDS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46143
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.028"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(28..300)
                     /locus_tag="KLH32_001634"
                     /pseudo
     CDS             complement(28..300)
                     /locus_tag="KLH32_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            451..2604
                     /locus_tag="KLH32_001635"
     CDS             451..2604
                     /locus_tag="KLH32_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE0138504.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2657..4387
                     /locus_tag="KLH32_001636"
     CDS             2657..4387
                     /locus_tag="KLH32_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138505.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4447..4938)
                     /gene="yajQ"
                     /locus_tag="KLH32_001637"
     CDS             complement(4447..4938)
                     /gene="yajQ"
                     /locus_tag="KLH32_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBE0138506.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            5106..6017
                     /gene="panE"
                     /locus_tag="KLH32_001638"
     CDS             5106..6017
                     /gene="panE"
                     /locus_tag="KLH32_001638"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE0138507.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5980..6570
                     /gene="yajL"
                     /locus_tag="KLH32_001639"
     CDS             5980..6570
                     /gene="yajL"
                     /locus_tag="KLH32_001639"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBE0138508.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6624..8072)
                     /gene="thiI"
                     /locus_tag="KLH32_001640"
     CDS             complement(6624..8072)
                     /gene="thiI"
                     /locus_tag="KLH32_001640"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBE0138509.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            8278..8520
                     /gene="xseB"
                     /locus_tag="KLH32_001641"
     CDS             8278..8520
                     /gene="xseB"
                     /locus_tag="KLH32_001641"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBE0138510.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8520..9419
                     /gene="ispA"
                     /locus_tag="KLH32_001642"
     CDS             8520..9419
                     /gene="ispA"
                     /locus_tag="KLH32_001642"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBE0138511.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9444..11306
                     /gene="dxs"
                     /locus_tag="KLH32_001643"
     CDS             9444..11306
                     /gene="dxs"
                     /locus_tag="KLH32_001643"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBE0138512.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11361..12278
                     /gene="yajO"
                     /locus_tag="KLH32_001644"
     CDS             11361..12278
                     /gene="yajO"
                     /locus_tag="KLH32_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBE0138513.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12389..12907)
                     /gene="pgpA"
                     /locus_tag="KLH32_001645"
     CDS             complement(12389..12907)
                     /gene="pgpA"
                     /locus_tag="KLH32_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBE0138514.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12885..13862)
                     /gene="thiL"
                     /locus_tag="KLH32_001646"
     CDS             complement(12885..13862)
                     /gene="thiL"
                     /locus_tag="KLH32_001646"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBE0138515.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13940..14359)
                     /gene="nusB"
                     /locus_tag="KLH32_001647"
     CDS             complement(13940..14359)
                     /gene="nusB"
                     /locus_tag="KLH32_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBE0138516.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14379..14849)
                     /gene="ribE"
                     /locus_tag="KLH32_001648"
     CDS             complement(14379..14849)
                     /gene="ribE"
                     /locus_tag="KLH32_001648"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBE0138517.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14938..16041)
                     /gene="ribD"
                     /locus_tag="KLH32_001649"
     CDS             complement(14938..16041)
                     /gene="ribD"
                     /locus_tag="KLH32_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBE0138518.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(16045..16494)
                     /gene="nrdR"
                     /locus_tag="KLH32_001650"
     CDS             complement(16045..16494)
                     /gene="nrdR"
                     /locus_tag="KLH32_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBE0138519.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16645..17184
                     /locus_tag="KLH32_001651"
     CDS             16645..17184
                     /locus_tag="KLH32_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBE0138520.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17482..18366
                     /gene="tsx"
                     /locus_tag="KLH32_001652"
     CDS             17482..18366
                     /gene="tsx"
                     /locus_tag="KLH32_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBE0138521.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18543..18890)
                     /locus_tag="KLH32_001653"
     CDS             complement(18543..18890)
                     /locus_tag="KLH32_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBE0138522.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(19019..19990)
                     /gene="secF"
                     /locus_tag="KLH32_001654"
     CDS             complement(19019..19990)
                     /gene="secF"
                     /locus_tag="KLH32_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBE0138523.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(20001..21848)
                     /gene="secD"
                     /locus_tag="KLH32_001655"
     CDS             complement(20001..21848)
                     /gene="secD"
                     /locus_tag="KLH32_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBE0138524.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21876..22208)
                     /gene="yajC"
                     /locus_tag="KLH32_001656"
     CDS             complement(21876..22208)
                     /gene="yajC"
                     /locus_tag="KLH32_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBE0138525.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(22231..23358)
                     /gene="tgt"
                     /locus_tag="KLH32_001657"
     CDS             complement(22231..23358)
                     /gene="tgt"
                     /locus_tag="KLH32_001657"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBE0138526.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23413..24483)
                     /gene="queA"
                     /locus_tag="KLH32_001658"
     CDS             complement(23413..24483)
                     /gene="queA"
                     /locus_tag="KLH32_001658"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBE0138527.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24576..25149
                     /gene="acpH"
                     /locus_tag="KLH32_001659"
                     /pseudo
     CDS             24576..25149
                     /gene="acpH"
                     /locus_tag="KLH32_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(25154..26971)
                     /gene="malZ"
                     /locus_tag="KLH32_001660"
     CDS             complement(25154..26971)
                     /gene="malZ"
                     /locus_tag="KLH32_001660"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBE0138528.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(27127..28500)
                     /gene="proY"
                     /locus_tag="KLH32_001661"
     CDS             complement(27127..28500)
                     /gene="proY"
                     /locus_tag="KLH32_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBE0138529.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28576..29895)
                     /gene="brnQ"
                     /locus_tag="KLH32_001662"
     CDS             complement(28576..29895)
                     /gene="brnQ"
                     /locus_tag="KLH32_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBE0138530.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(30302..31597)
                     /gene="phoR"
                     /locus_tag="KLH32_001663"
     CDS             complement(30302..31597)
                     /gene="phoR"
                     /locus_tag="KLH32_001663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBE0138531.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31655..32344)
                     /gene="phoB"
                     /locus_tag="KLH32_001664"
                     /pseudo
     CDS             complement(31655..32344)
                     /gene="phoB"
                     /locus_tag="KLH32_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32534..33736
                     /gene="sbcD"
                     /locus_tag="KLH32_001665"
     CDS             32534..33736
                     /gene="sbcD"
                     /locus_tag="KLH32_001665"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBE0138532.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33733..36876
                     /gene="sbcC"
                     /locus_tag="KLH32_001666"
     CDS             33733..36876
                     /gene="sbcC"
                     /locus_tag="KLH32_001666"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBE0138533.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            37002..38183
                     /gene="araJ"
                     /locus_tag="KLH32_001667"
     CDS             37002..38183
                     /gene="araJ"
                     /locus_tag="KLH32_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBE0138534.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(38326..39234)
                     /gene="mak"
                     /locus_tag="KLH32_001668"
     CDS             complement(38326..39234)
                     /gene="mak"
                     /locus_tag="KLH32_001668"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBE0138535.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39359..40270
                     /gene="rdgC"
                     /locus_tag="KLH32_001669"
     CDS             39359..40270
                     /gene="rdgC"
                     /locus_tag="KLH32_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBE0138536.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40348..40803)
                     /locus_tag="KLH32_001670"
     CDS             complement(40348..40803)
                     /locus_tag="KLH32_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138537.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40800..41135)
                     /locus_tag="KLH32_001671"
                     /pseudo
     CDS             complement(40800..41135)
                     /locus_tag="KLH32_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(41290..41574)
                     /gene="ppnP"
                     /locus_tag="KLH32_001672"
     CDS             complement(41290..41574)
                     /gene="ppnP"
                     /locus_tag="KLH32_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBE0138538.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41646..42323)
                     /locus_tag="KLH32_001673"
     CDS             complement(41646..42323)
                     /locus_tag="KLH32_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBE0138539.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42584..42772)
                     /gene="yaiA"
                     /locus_tag="KLH32_001674"
     CDS             complement(42584..42772)
                     /gene="yaiA"
                     /locus_tag="KLH32_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBE0138540.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42822..43346)
                     /gene="aroL"
                     /locus_tag="KLH32_001675"
     CDS             complement(42822..43346)
                     /gene="aroL"
                     /locus_tag="KLH32_001675"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBE0138541.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43529..43987)
                     /locus_tag="KLH32_001676"
     CDS             complement(43529..43987)
                     /locus_tag="KLH32_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBE0138542.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            44107..44916
                     /gene="proC"
                     /locus_tag="KLH32_001677"
     CDS             44107..44916
                     /gene="proC"
                     /locus_tag="KLH32_001677"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBE0138543.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44933..46048)
                     /gene="adrA"
                     /locus_tag="KLH32_001678"
                     /gene_synonym="dgcC"
     CDS             complement(44933..46048)
                     /gene="adrA"
                     /locus_tag="KLH32_001678"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBE0138544.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11996 a        12026 c        11294 g        10827 t
ORIGIN      
        1 cactgcccgg ttgtatgcgc gaggttaccg actgcggcct gagtttttta agtgacgtaa
       61 aaccgtgttg aggccaacgc ccataatgcg tgcactggcg cgacatccga cgccattcat
      121 ggccatatca atgattttct ggtgcgtacc gggctgagag gcggtgtaag tgaactgtag
      181 ttgccatgtt ttacgacaat gagagcagag attgcgctga tgtccggcag tgcttttgcc
      241 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
      301 gcaagcacct taaaatcacc atcatacact aaatcaataa gttggcagca ttaccttcgc
      361 ttaattccat ataactggac gattacatta gaagtaaact atacttttta tgaaatatca
      421 gggataaatt attcacatgg aaaatacatc atgtacaaat cattatttac tattatcatt
      481 ctactgtata gcgccagttc atatgcaaaa agcgaatccc ctcccgcaag cactataact
      541 ttcattgcac aaatcgatca tgttgagcta aataaaacag cattatctca tgatatgaaa
      601 ctcgttcttg ccgatcgttt ttatttcaaa accagtaatc cttgcgaaac aaattcaatt
      661 tctgctcgac cagaatcaat ggatattact gacaaagcct atgccgacaa attaaaaaaa
      721 ttagaaactc cttttcttga tgatcgctat ttctttatga ccatcaatca gtgtgacggt
      781 aatggcactc ccatgctgac agacataaaa gtatgtacaa cagaattatg tggtgctgaa
      841 tatacaaaga aaatgtctac tgtatggctc gatgaaactt taaaaccaac tgctaaacgt
      901 ctggcaattt attttattca atctccttta ccctatgacg aggagaaaaa acaatggaaa
      961 gtcaccggat ggtatatctc atcaactgat aatcagtctt caactaaaca gattgcattt
     1021 gaaggatata cagacacaga taacctctcc gattctaaat ttatttcttc atttaaatcc
     1081 tggtttgaaa gtggaaagat aaaaaacatt gctaactata atccaaaagg agaaagagat
     1141 ggaaaatacg aaagttatta caatagcaat ggtaacgtct atgaatctct aagtttttct
     1201 aatggtctta tcaacggtga gtttattaca tattatgaaa atggggccat tgaaagtaaa
     1261 cgtcatttta ataacgacaa aattaaagat ggtgaatgcc ctcattttta tgaagatggg
     1321 aaaatcaaac agcaacatag ctatctgaat agtaaactcg atggtcctgc atatgaatat
     1381 tatcctgatg gcaaactcat gcaggaaaat ttctatcagc aaagtgtgtt gattggaaac
     1441 gataccagtt actaccaatc cggaaaaata agctctatac acaatagaaa ttcccgtggt
     1501 cagtatgatg gagctaacga acgttatagt gaagagggta agttactgtc taaatccgtc
     1561 tataaagacg gaaaacaaat ttctgttcaa acctggtatg aaaatgggca gaaagaagag
     1621 gagaaacatt ttgatgagca aggccaatta aatggtctgg ttaagcaatg gtataaaaat
     1681 ggtaatcttg caaaatcaca aaactataaa catgacatac tcgatggtaa cagtgaagag
     1741 tggtacgaaa atggcatccc tgaaagtttg tacccttata agaatggaaa aaccgacggc
     1801 attgccaaat catggaataa gtatggtaaa ttaacctatt caatcgaata caaaaatggc
     1861 gtagagaatg gggcatatcg taactggagc aaaaacacag gtaaactaac caaagaaacc
     1921 ctgtacgtca atggtatcag acagggagtg gaaaaagagt ttaacgatcg cacaggtaaa
     1981 ctattaacct ccacgcaata tgttaataat aaaagacatg gtacggagga aacttatgat
     2041 caaaacggaa taaaatatat tacatgctat caaaatgatc agaaattatc gtctttagac
     2101 aatccaacac aaataaaaga taatgcaaca acaggcgatt ccagtgctca atttgcatta
     2161 gggaaatacg aattcatctg tgcaaatata gatgaaggga ttaaatggtt aaccaaatct
     2221 gcagagcaaa aaaacactga tgcgatatac tttttagcta cagcctacaa aggcaatggt
     2281 atacctgcaa acaatgaaaa atacctagca tatctgcaac aagctgcaac actgggcagt
     2341 agtaatgccc aggcggaaat tggctattta tatctcatcg gaaaagaact accacagaat
     2401 cttcctgatg ctggtgtctg gttcaaaaaa gcagctgcac aaggtaattt tgccgcacat
     2461 ttctatctgg gaaggatgta tcagaatggt gacggcgttg agagaaatat ggaaaaagcc
     2521 aggtttcatc tatctaatgc ggcagaaggt ggaatagaac ctgcgcttaa agcattaaat
     2581 gaacttgaac atcaaacaaa ataaagcgct cagggaccac gtaaggttca tcaaacagaa
     2641 aaggatatag tttgcgatga aagcgaaatc tactttgtta ttaatgattt tatctgtttt
     2701 ttattctcat caggttttat ccagcgaacc cactcagccg gatgtttatt ctgtcgtcga
     2761 gaaaaaacta aactctgctt tacctctgga agaaaatacc accttcagat cgcaggctga
     2821 atggtttaat ttgcaatatg aattattagt atctggctac cctgaacgtg cttacaattt
     2881 actgagcaaa cttgagacag aatcgaaaaa cgctaaatat tatctggatt taactgcctc
     2941 cagagtcgct gaagaagaag caccagagac agccttaact tttttatcaa aaattgggtt
     3001 aaataaaccg gctactctta catcctttga gagctatatc aatagctgga taaaaagcaa
     3061 acaacccgaa aaggctatgc agcttctcag cctcgatgag aatgccctga attattattt
     3121 accgactgtg ttagtcgcat atcagatcgt tcctgataag gcaataagta tttataattc
     3181 tgtatatgga gataatgttg ttatacccta tcagcaagtc accatgttac ttacagttgc
     3241 ggagggttat caaacaaaag gcgatataaa aaacgccatt cattatgccg ataaggcgct
     3301 gaatatgttt gataaagcta ttgctgtaga tagcagtcgt gaatcccatt atagtgatga
     3361 gtatttgaaa ctcatgaata tctacgccgc caatggcaac aaagaaaaag cggtggtttt
     3421 atctcaacgt ctacacaaag ccattaccga atctcaatcg aattacttaa gtactctgga
     3481 aggaatttta ttgttttata gaaataatga tatgcagcaa gactatcaaa acagcctttc
     3541 aaattacata gtatttatcg ataaaatttt tgccttttcc ccatcaacaa gaagtgaatt
     3601 aagcctcatt aatttattaa attcattaaa tgaggtggaa ttgatgagaa aacgtctggc
     3661 gtttctgatg acagcaccag aatacgcttg ttatgatagt cagtattgtt atgaagacaa
     3721 agtcagcgca ttaaaaatcc tgtatcgtca tcatgacaat gagctaatgg acaaatattt
     3781 cacgactctt cttaaagaga tcagtaatca accattagaa gactgggata taataataag
     3841 taatactggt gaaaaattta gtgaagtggg tctaaccaaa tatgctcaac atctcgcatc
     3901 tttcgctgaa gaattttata taaaagagca aaaatcacat tctgaaagtg aaatgagagg
     3961 actattttca tcacttgccg aattatatag ttttggtaat gatactgcca gcgcaaaccg
     4021 agtgttccat cagcatgtcc ctgccttcaa tacagatcat atgattcaat attttataaa
     4081 tgccaaagaa tggaacaatg ccagggaatt attgataaaa gaggaaatgt tgggtatgca
     4141 taatctgata ttgttggaga atatttgtat gcaaaaaaat gcagaatgca tggcacacat
     4201 tacctttacg cttaataaat taacaacaca accagcaata acaaaaatag atagcgtggg
     4261 taatgagcaa ctttatcaga ttggcaaaat ctaccataag ttggaaatta aacctgagcc
     4321 agagcaacag gttttaattc agtcgcttta tgaccgggca tcaggaagcg cctctgcaac
     4381 tcaataaatg catggtcaaa agggccacca cggtggccct gacaatcagt agctatatcc
     4441 agaaagttaa tcgcggaagt ttttgaactg gaacggctga ccgagatcgc caccgcgcac
     4501 catcgccatg acagcctgca aatcatcacg agatttgcca gtcacgcgga tctcatcgcc
     4561 ctgaatttgc gcctgcactt tcagtttgct gtctttgatc atcttgacga tttttttctg
     4621 agtggcgctc tcaatgccct gtttcagttt cgcttccaca aaccaggttt taccgctatg
     4681 aacgatattt tccggtacat ccagtgaact gccttcaatg ccgcgcttca gcagcttggc
     4741 acgcagaata tccagcaact gattgacctg gaagtcggac tcgctcaaca ctttgatggt
     4801 tttgctggcg tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa aacgggactc
     4861 cacttcgcga ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa cttcagagac
     4921 aatatcgaaa gatggcatct tttcctctcc cttcattttt gatgcgaagc ataatacctg
     4981 caaagttaaa taaccaacag tcctgcacca ggacgtatcc cttaattgtg catagtcgca
     5041 acagcatgca caattaaggg atagccttat tgaacacccg gagtggttgc gggtgaggag
     5101 gaacaatgaa aattaccgta ttgggatgcg gtgccttagg gcaattatgg cttacagcac
     5161 tttgtaaaca gggtcatgaa gttcagggct ggctgcgtgt accgcaacct tattgtagtg
     5221 tgaatctggt tgagacagat ggttcgatat ttaacgaatc gctgaccgcc aacgatcccg
     5281 attttctcgc caccagcgat ctgctcctgg tgacgctgaa agcatggcag gtttccgatg
     5341 ccgtcaaaag cctcgcatcc acactgcctg taactacgcc aatactgtta attcacaacg
     5401 gcatgggcac catcgaagaa ttgcaaaaca ttcagcagcc attactgatg ggcaccacca
     5461 cccatgccgc ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga atcacgcata
     5521 tcggtccggc acggcaacag gatggcgatt acagttatct ggcggatatt ttgcaaaccg
     5581 tgttgcctga cgttgcctgg cataacaata ttcgcgccga gctgtggcgc aagctggccg
     5641 tcaactgcgt gattaatcca ctgactgcca tctggaattg cccgaacggt gaattacgtc
     5701 atcatccgca agaaattatg caaatatgcg aagaagtcgc ggcagtgatc gaacgcgaag
     5761 ggcatcatac ttcagcggaa gatttgcgtg attacgtgat gcaggtgatt gatgccacag
     5821 cagaaaatat ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc gaaatcgact
     5881 atatcaatgg ttttctctta cgccgcgccc gcgcgcatgg gattgccgta ccggaaaaca
     5941 cccgcctgtt tgaaatggta aaaagaaagg agagtgaata tgagcgcatc ggcactggtt
     6001 tgcctcgccc ctggtagtga agagactgaa gccgtcacca cgatcaacct cttggttcgc
     6061 ggcggtatca aagtcaccac tgccagcgtc gacagcgatg gcaacctggc aattacctgc
     6121 tcgcgcggcg tgaagctgct ggcggatgcg ccgctggtcg aagtggctga tggcgaatat
     6181 gacgtgatcg tgctgcctgg tggcattaaa ggcacggagt gttttcgcga tagcactctg
     6241 ctggttgaaa ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc tatttgcgcc
     6301 gcgccagcca ccgtgctggt gccgcacgat atctttccga ttggcaatat gaccggcttc
     6361 ccgacgctga aagacaaaat tcccgctgaa caatggcagg acaagcgcgt cgtctgggat
     6421 gcacgggtaa aattgctgac cagccagggg ccgggtacag ctatcgactt tggtctgaaa
     6481 attatcgacc tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca actggtgatg
     6541 gcggcaggga tttataatta ttacgagtag tgtcggatgc ggcaaacgtc gcatctgacc
     6601 agatgcgacg taaaaactac aaattacggg cgatatacct tcacattgtt aaagccttgc
     6661 tcgcgcagat agagcgcctg cagacggctc atcaccccgc gctcacacca cagcagccag
     6721 gttttgttct ggtcgagatc gccaaatttg gtgctcagtt tatagaacgg cagagaaacc
     6781 acatcaatcc cttcgacttt cagtggctta tcttcctgtt catcgacaga acggatatcg
     6841 agaatcacgt cgttcgggcc gaagccgttg acggtttcca cttccaccac ttcctgctcg
     6901 gtctgctggg cgatttcgcg gatatcaacg ttattcgctt cctcaaccac tttatcgaga
     6961 atactgaagt cgaacttctc ttcttccgct tcaatcttcg atttaaccgc tttcaccgtc
     7021 gggcttttgg agatcacgcc acaatattcc ggcatcgtgc gagcaaagtc ttcggtgcca
     7081 atctggcgag ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg acgcaggatc
     7141 agcgtgtcag agacgttatc aatcaggcgc aggttggtca gcgtctgact ggacacctgg
     7201 ccgagcgctt cgccggtgac cagcgcctgt acgccgtaac gttcagccac tttagatgcg
     7261 gcacgcacca tcatacgttt gaggataacg cccatctgac cgtcgtcgat tttctcgaga
     7321 atttccccga caaccggttc gaaattaatg gcgacaaaac gcacgcggtg ggagctgcca
     7381 aagcggttcc acagataatg cgccacctga cgcacgccaa tttcatgcgc tgcgccgccg
     7441 aggttaaaga agcagtaatg cacgcggcag ccgcgacgca tcaacatata actggaaaca
     7501 ccggagtcga aaccaccgga aatgagcgac agcacatctt cctgggtgcc aatcgggaaa
     7561 ccaccaatac cttcgtagcg acctttaatc agcaggaggc gatcgtcttc cacttccaga
     7621 tgaacagtta catccgggtt agtcagcttc acgcgcgcgg attcaatatg ctgatttaaa
     7681 ccgccgccga cgtaacgttc cacatcaatc gagctaaaat catgtttgcc acggcgcttc
     7741 acgcgtacgc agaaggtttt gccttccagc tgatcgcgat actgaaccaa cgctttctca
     7801 aaaatatcgt gcatgtcggt aaacggcacg tcttcgactt cgagaatatg gtggataccc
     7861 ggaatacggg tcagggcgtc gcgaatagtc agacgctggt tttcatcttt cgcgcgaact
     7921 tcgatgttat cccagtggcg gacgacagcg agcgtctcat catagtgctt taaaacgtta
     7981 cgaatgttcc cggtaaggat ttttataaag cgcaagcgca cagattggct tttgatggtg
     8041 atttccggga acaatttaat gataaacttc atggcggcaa tggttcgttg gcaagcctta
     8101 agcgacttgt atagggaaaa atacagcagc ccacacctgc ggctgcatcc aggcgcggaa
     8161 gtataccact aacatcgctt tgctgcgcac atcaccttac cattgcgcgt tatttgctat
     8221 ttgccctgag tccgttacca tgacggggcg aaaaatattg agagtcagac attcattatg
     8281 ccgaagaaaa atgaggcgcc cgccagcttt gaaaaggcgc tgagcgagct ggaacagatt
     8341 gtaacccgtc tggaaagtgg cgacctgccg ctggaagagg cgctgaacga gttcgaacgc
     8401 ggcgtgcagc tggcacgtca ggggcaggcc aaattacaac aagccgaaca gcgcgtacaa
     8461 attctgctgt ctgacaatga agacgcctct ctaacccctt ttacaccgga caatgagtaa
     8521 tggactttcc gcagcaactc gaagcctgcg ttaagcaggc caaccaggcg ctgagccgtt
     8581 ttatcgcccc actgcccttt cagaacactc ccgtggtcga aaccatgcag tatggcgcat
     8641 tattaggtgg taagcgcctg cgacctttcc tggtttatgc caccggtcat atgtttggcg
     8701 ttagcacaaa cacgctggac gcacccgctg ctgccgtaga gtgtatccac gcttactcat
     8761 taattcatga tgatttaccg gcgatggatg atgacgatct gcgtcgcggt ttgccgacct
     8821 gccatgtgaa gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta caaacgctgg
     8881 cgttctcgat tctaagcgat gccaatatgc cggaagtgtc ggatcgcgac agaatttcga
     8941 tgatttctga actggcgagc gccagcggta ttgccggaat gtgcggtggt caggcattag
     9001 atttagacgc ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt catcgtcata
     9061 aaaccggcgc attgattcgc gccgccgttc gccttggtgc attaagcgcc ggagataaag
     9121 ggcgtcgtgc tctgccagta ctcgacaagt acgcagagag catcggcctt gccttccagg
     9181 ttcaggatga catcctggat gtggtaggag atactgcaac gttgggaaaa cgccagggtg
     9241 ccgaccagca acttggtaaa agtacctacc ctgcacttct gggtcttgag caagcccgga
     9301 agaaagcccg ggatctgatc gacgatgccc gtcaggcgct gaaacaactg gctgaacagt
     9361 cactcgatac ctcggcactg gaagcgctag cggactacat catccagcgt aataaataaa
     9421 caataagtat taataggccc ctgatgagtt ttgatattgc caaatacccg accctggcac
     9481 tggtcgactc cacccaggag ttacgactgt tgccgaaaga gagtttaccg aaactctgcg
     9541 acgaactgcg ccgctattta ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg
     9601 ggctgggcac ggtcgaactg accgtggcgc tgcactatgt ctacaacacc ccatttgacc
     9661 aattgatttg ggatgtggga catcaggctt atccgcataa aattttgacc ggacgccgcg
     9721 acaaaatcgg caccatccgt cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa
     9781 gcgaatatga cgtattaagc gtcgggcatt catcaacctc catcagtgcc ggaattggta
     9841 ttgcggttgc tgccgagaaa gaaggcaaaa attgccgcac cgtctgtgtg attggcgacg
     9901 gcgcgattac cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat attcgccctg
     9961 atatgctggt ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtt ggcgcgctta
    10021 acaaccatct ggctcagctg ctttccggta agctttactc ttcactgcgc gaaggcggga
    10081 aaaaagtttt ctctggcgtg ccgcccatta aagagctgct caaacgtacc gaagaacata
    10141 ttaaaggcat ggtggtgcct ggcacgttgt ttgaagagct gggatttaac tacatcggcc
    10201 cggtggacgg tcacgatgtg ctggggctta tcaccacgct aaagaacatg cgcgacctga
    10261 aaggcccgca gttcctgcat atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa
    10321 aagacccgat cactttccac gccgtgccta aatttgatcc ctccagcggt tgtttgccga
    10381 aaagtagcgg cggtttgccg agctattcaa aaatctttgg cgactggttg tgcgaaacgg
    10441 cagcgaaaga caacaagctg atggcgatta ctccggcgat gcgtgaaggt tccggcatgg
    10501 tcgagttttc acgtaaattc ccggatcgtt acttcgacgt ggctattgcc gagcaacacg
    10561 cagtgacctt tgccgccggt ctggcgattg gtggatacaa acccattgtc gcgatctact
    10621 ccaccttcct gcaacgcgcc tatgatcagg tgctgcatga cgtggcaatt caaaagctac
    10681 cggtcctgtt cgccattgac cgcgcgggca ttgttggtgc tgacggtcaa actcaccagg
    10741 gcgcttttga tctctcttac ctgcgctgca taccagaaat ggtcattatg accccgagcg
    10801 atgaaaacga atgtcgccag atgctctata ccggctatca ctataacgat ggcccgtccg
    10861 cggtgcgcta cccgcgcggc aacgcggttg gcgtggaact gacgccgctg gaaaaactac
    10921 caattggcaa aggcattgtg aagcgtcgtg gcgagaaact ggtgatcctt aactttggta
    10981 cgctgatgcc agaaacggcc aaagtcgccg aatcgctgaa cgctacgctg gtcgatatgc
    11041 gttttgtgaa accacttgat gaagcgttaa ttctggaaat ggccgccagc catgaagcgc
    11101 tggtcaccgt agaagaaaac gccattatgg gcggcgcagg tagcggagtg aacgaagtgc
    11161 tgatggccca tcgtaaacca gtacccgtgc tgaacattgg cctgccggac ttctttattc
    11221 cgcaaggaac tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc ggtatggaag
    11281 ccaaaatcaa ggcctggctg gcataatccc tactccactc ctgctatgct taagaaatta
    11341 ttcatggcag gagtggcagc atgcaataca accccttagg aaaaaccgac cttcgcgttt
    11401 cccgactttg cctcggctgt atgacctttg gcgagccaga tcgcggtaat cacgcatgga
    11461 cactgccgga agagagcagc cgtcccataa ttaaacgcgc gctggaaggt ggcataaatt
    11521 tctttgatac cgccaatagc tattccgatg gtagcagcga agagatcgtt ggtcgcgcac
    11581 tgcgggattt cgcccgtcgt gaagacgtag tcgttgccac caaagtgttc catcgcgttg
    11641 gtgatttacc ggaaggatta tcccgtgcac aaattttgcg ctctatcaac gacagcctgc
    11701 gacgtctcgg catggattat gtcgatatcc tgcaaattca tcgctgggat tacaacacgc
    11761 cgatcgaaga gacgctggaa gccctgaacg acgtggtaaa agccgggaaa gcgcgttata
    11821 tcggcgcgtc atccatgcac gcttcgcagt ttgctcaggc gctggaacta caaaaacagc
    11881 actgctgggc gcagtttgtc agtatgcagg atcactataa tctgatttat cgcgaagaag
    11941 agcgcgagat gctgcctctg tgttatcagg agggcgtggc ggtgattccg tggagcccgc
    12001 tggcgcgggg gcgactgacg cgtccatggg gagaaactac cgcacgactg gtgtctgatg
    12061 aggtggggaa aaatctctat aaagaaagcg atgaaaatga cgcgcagatc gcagagcggt
    12121 taacgggcgt cagtgaagaa ctcggtgcaa cacgagcaca agttgcgctg gcctggttgt
    12181 taagtaaacc gggcattgcc gcaccgatta tcggaacttc gcgggaagaa cagcttgatg
    12241 agctgctgaa cgcggtggat atcactttga agccataaca gattgccgag ctggaaacgc
    12301 cgtataaacc gcatgcggtc gtaggattta aataagagtc caggcctgat gagacgtgac
    12361 aagcgtcaca tcaggcatcg gtgcacaact acgacagaat acccagcggc cagtgatgac
    12421 cgataaagta caggatgcct gcggaaatca ccccggcgac aatatcgtcg atcatgatcc
    12481 ccatgccgcc atgtacattg cgatcaaacc agcggatcgg ccacggcttc cacatatcca
    12541 gaatacggaa aatcacaaac ccggcggcaa cccactgcca gtcattggtc ggcagcgcca
    12601 tgagcgtgat ccacatacca ataaattcgt cccagacaat gctgccgtga tcgtgcacgc
    12661 ccatatcttt cgccgtttga tgacaaagat agacgccgat acagatcccc aacatcacca
    12721 ccagcgagta gagctgccag ggtaaaaagg tcatcagata ccagaaaggg atcgccgcca
    12781 gcgatcccat cgtgccagga acgattgggc ttaatccact tccgaatccg acagcaagca
    12841 gatgccacgg attactcatc ttcaggcgac ttttcgcgac atctttatgg cgtgacaaaa
    12901 tggtcatatc ctttccagtc aaacgtgaca ggttcgccgt cacgaataaa acaaagccct
    12961 tcgatatcgg cggtcatttg cccgatacag gtaaacggta cgccaaggtg tccgagagca
    13021 acatccagcg cgccacggtt cagttccggc accgtgaaac acaactcgta atcttcaccg
    13081 ccagagagcg cccagcgcag cgcctgttcc ggttcaacat ggcgagaaag cgcatcagaa
    13141 aacggcagca atgccaggtc aatacgtgcg ccgcagtcgc tggctttcac gatatgcccg
    13201 agatcggaaa tcagaccgtc agagagatcg atagctgaat ttgccagatc gcgcagtgcc
    13261 tgcccctgta aaatacgcgg cgatggacgg agatgacgtt tgatcaagta gtcagcatct
    13321 ttagcatcgg caacctgcaa acggttttgc aaaatcgcca gcccggcggc gctatcgccc
    13381 ggtgtaccgg tcacatagat ccagtcaccc ggtttcgccc cagagcgcgt taaggctcgt
    13441 cccatcggaa caaagccgtg gatacccaac gtcattgata atggcccacg cgtggtatcg
    13501 ccgccaatga gttgcatatc gtaataattg agaagatcaa acaaactgtc gctgaaggac
    13561 tcaagccacg cttcgtctac gtccggtaag gttaatgcca gcgtcagcca ggccggatcg
    13621 gcccccatcg ctgccagatc gcttaggttc accgccagtg ctttataagc cagatcagca
    13681 ggatcgatat cagggaggaa atggtttccc gccaccagcg tatcagtgct gatcgccagg
    13741 gtctgtttct cggggatatt gagaagagcg caatcgtcgc cgatgcccag ttcgacatca
    13801 agacgagaac ttcttacacg gtcaaaataa cgggcaatca gggagaactc gccacatgcc
    13861 atacgttatg cctcagcagg taaaaaagaa aaggccggcg atcatggaac agtcttccgt
    13921 gaatctaccg gcctggatat tactttttgt tagggcgaat cacaggtgct gctttatcga
    13981 gtacgccgtt gacgaactta tggctgtctt ctgcgccgaa cgatttcgcc agttcgatcg
    14041 cttcgttaat ggccactttg tatggcacat cactacgttt agacagttcg tacagcgcaa
    14101 tgcgcagtac tgctttttct acctggccca gttcttccag caggcgggac aggtagggct
    14161 tcatcagtcc gtcgaggtat gcggtgttag tcgccacccc ggccagcagc tcacggaagt
    14221 acaggacgtc aacgtctttt acatcctgtt cagccaggaa ctggtattca acatcagcga
    14281 tgtcgttctg ggacaactgc caggagtaga gcgcctggac ggcacactca cgagcgcggc
    14341 gacgagcagc aggtttcacg gatttcccct tactaatttc aggccttgat ggctttcaat
    14401 acattaatca tttcaagcgc ggtcagtgca gcttctgcac ctttgttgcc agctttggtg
    14461 ccagcacgtt cgatcgcttg ttcaatgctt tcagtggtca gaaccccaaa agcaaccgga
    14521 atttcgctgt cctgggcaac atgcgccagg ccgttgcttg caccaccagc gacatattca
    14581 aagtgggcag tgccaccacg aataaccgta cccagcgcaa tcaccgcgtc gtatttaccg
    14641 gttttagcca gtgcacccgc cgccagcggc agctcatagg caccaggcac ccaaacaacg
    14701 gtaatgtttt catcttttac ctgaccgata cgtttcagtg cgtcaattgc accttccagc
    14761 aggctgtcat tgataaagtt gttgaaacgc gcgatggtga tggcgacgcg agcgtccggg
    14821 gtagcaacgt tagcttcaat aatgttcatt ttcttccttc gggttcgagt atggccccgc
    14881 aggggggcgg attttagcat aatatttcgt gcgctgcttc cctttcgagc cgggagatca
    14941 tgcacccact aaatgcaggc aaacatccgg gcctacatga cgtatctctt tgaatttaaa
    15001 ttggggggcg tcggctaatt tctcaagccc tggcagcgag cataatccac gggcgtcgct
    15061 gcctaatagt ttaggtgcga tatagacaat cagctcatcg actaaacccg cctgtagcaa
    15121 tacgccagcg agcgttggcc ccgcttccac ccagatgctg ttaatctgct gtttacccag
    15181 ttgcatcatc agtacaacca gatccagatg acctttatgc tctggaatca gcaaggtacg
    15241 caccgtttcc ggccactcac gagaatcttc ctgcgtacgc gcgaaccagg tttcgccggg
    15301 ctgctgcaca atgcgatgtt ccggcgtcac gcgattttgg ctatcaatca caatacgtac
    15361 tggctggcgg agattttgtt gcgggtagag cgcctgagtt tgttcatcca gttcagacca
    15421 acgcactgtt aaggctggat catccgccag caccgtggcg ctgctggtta aaatggcatg
    15481 actttgtgcg cgctgtcgtt gtacgtcgcg ccgcgcctga ggcgaagtga tccactggct
    15541 ttcgccgctc gccatcgccg tgcggccatc aagcgatgcg ccaagtttta actgaatata
    15601 aggaaagccg gtgcgcatcc gcttgagaaa gcctttattc aattgctcgg cttcactcat
    15661 catcagcccg tggctgacgt caatgccagc ctgttgcaga cggtaaagtc cacgcccagc
    15721 gacctgcggg ttaggatctt gcatcgctgc gaccacgcgc gctacgccag cggcgatgag
    15781 tgcgtcacag cacggtggcg tacggccatg atggctacag ggttcgagtg tcacatacgc
    15841 cgttgcgcct ttggcttttt cacccgccat acgcaacgcg tgtacttcgg catgtggttc
    15901 acccgctcgt tggtggtaac cttcaccgac aatttcgcca tctttgacaa tgacgcaccc
    15961 gacattcggg ttggggtggg tggtaaaacg tcctcgttgc gccagcttta gcgcccgcgc
    16021 catgtaatac tcgtcctgca cggcttagtc ctccaggcgc gcgatctctt cgccaaattc
    16081 tttgatatct tcgaaactgc ggtagacaga ggcaaaacgg atataggcga ctttatcgag
    16141 ctttttcaat tgctccatca ccagattgcc aatcatcttg ctcggtactt cgcgctcacc
    16201 ggtggcgcgc agctgcgatt taatatgatt gattgccatt tcaacgtaat cggaactcac
    16261 cggacgtttt tccagcgccc gcagcattcc gctacgcaat ttctcttcat taaacggttc
    16321 acgcacgtcg ttgcttttta caacacgcgg cataaccagc tccgccactt caaaggtggt
    16381 gaaacgttca ttacacacca gacactgccg acggcggcgt acggatgaac cctcgcccac
    16441 gagacgagag tcaattacct tagtgtccac ggcgaaacag aatgggcaat gcatacggcg
    16501 tcctgaccag gtggttaacg gaaatctatt ttaccctgaa ctgacgatac aacaaaggcg
    16561 caacgctttt gagataaagg atcgatgctt tcgctgcttt taccctctac cattaggcta
    16621 attgcgacga gaaagggacg acctatgaat actaacgttt ttcgactgct cctgctggga
    16681 agcctgttca gccttagcgc ctgtgtgcag caaagtgaag tgcgacagat gaaacacagc
    16741 gtcagcacgc tgaaccagga gatgacgcaa ctcaatcaag aaacagtcaa aatcactcag
    16801 caaaacaggc tgaatgcaaa atccagcagt ggggtttacc ttctgcctgg agcgaaaaca
    16861 ccggcaagac tggaaagcca gatcggtact ttacgtatgt cgctggtgaa tattacgcct
    16921 gatacagatg gcaccaccct tacactacgt attcagggtg agtcgaatga tcccttgccc
    16981 gcattcagcg gaactgttga atatgggcaa attcagggaa caatagacaa ttttcaggaa
    17041 atcaatgtgc agaatcaatt gattaatgcc cctgccagcg tcctcgcccc cagcgatgtt
    17101 gatattccgt tacaattaaa ggggatttct gtggatcagt taggctttgt acgtatccac
    17161 gacattcaac ctgttatgca gtaaacgtat ttcgggacga ttttgtgcgt cccgcaacat
    17221 cttccccgtc attttgttac tctgcttaca tcacctggat tgatagtaaa agtttgcaac
    17281 aagggcgaaa gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg caatcgatta
    17341 cgtaaatgat agaactgtga aacgaaacat atttttgtga gcaatgattt ttataatagg
    17401 ctcctctgta tacgaaatat ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga
    17461 ttttcaaaca gtggcataca tatgaaaaaa acattactgg cagccggtgc ggtactggcg
    17521 ctctcttcgt cttttactgt caacgcagct gaaaacgaca aaccgcagta tctttccgac
    17581 tggtggcacc agagcgttaa cgttgtcgga agctatcaca cccgtttcgg accgcagatc
    17641 cgcaacgata cctaccttga gtacgaagca ttcgctaaaa aagactggtt cgacttctat
    17701 ggttatgcgg atgcgccggt attcttcggc ggtaactccg atgctaaagg tatctggaac
    17761 cacggttctc cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat
    17821 actgacctta gcttcggtcc gttcaaagag tggtacttcg cgaacaacta catttacgac
    17881 atgggtcgta ataaagatgg tcgccagagc acctggtaca tgggtctggg taccgatatc
    17941 gacactggcc tgccgatgag cctgtccatg aacgtctatg cgaaatacca gtggcagaac
    18001 tatggcgcag cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa atactttgtg
    18061 ccgattaccg atctgtgggg cggtcagctg agctacatcg gcttcaccaa cttcgactgg
    18121 ggttccgatt taggggatga cagcggtaac gcaatcaacg gtattaagac ccgtactaat
    18181 aactctatcg cttccagcca tattctggct ctgaactacg atcactggca ctactctgtc
    18241 gtagctcgtt actggcacga cggtggtcag tggaacgacg atgcagaact gaacttcggc
    18301 aacggcaact tcaacgttcg ctctaccggc tggggtggtt acctggtagt aggttacaac
    18361 ttctgattat gaaaatgccg ggatttattc ccggcatttc tgattgttaa caacgtgtgc
    18421 tttgttcatg ccggatgcgg cgtaaacgct ttgtccggcc cacaaaagca cgtaaattca
    18481 atatattgct gaaataacgt aggcctgata agcgtagcgc atcaggcaat tttacgtttt
    18541 aatcacttct tcttgttcat catcgctttc aggtcagcga atgggttgta cttcgcttca
    18601 ccgacatctt tctgcgcatc ttcccctgcg ataacggtcg taccatactg atccgcttcg
    18661 gtatatttcg aatgctcatg atcgtggcaa tagagacaca acaattccca gttactgcca
    18721 tcttccgggt tattggtatg gtcgtgatca atgtggtgaa cggtaagttc acgcaggtta
    18781 gaataaacaa actcgcggga acagcgaccg cagacccacg gatagatttt taatgctttt
    18841 tcgcgatagc cgctttctaa ccgtgcatag ttttttggga tgatagccat gaaaagttac
    18901 ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc gcaatctcca
    18961 tacatcaaaa aaaatcccga tcttctgacc gggattttca acatcaacgg gaacttgatt
    19021 aaggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc aacatgtgtt
    19081 cgcgcttcat acccagtttc agagccaacg cagatgctac gtagatggaa gatgcagtgc
    19141 cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga ccaccgaaga
    19201 gatacagcat caggataacc atcaaggtag taccggatgt gatcaaggta cggtgcagcg
    19261 tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt
    19321 tttcacgaat tcggtccgat accacgatac tgtcgttaag cgagtaaccg ataaccgaca
    19381 tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa atacccagcg
    19441 taatgataac gtcgtgcgtc agcgcaataa ccacccctgc cgccagtcgc cactcaaagc
    19501 ggaaacctac gtacacgagg atagacagca gcgctgccat caacgccatc gcaccggttt
    19561 gcgcaaggtc agcccccacg ctcggaccga cgaactcaat acgcttcact gctgcattct
    19621 gattggtgga ttcgttaatc accttcagaa cttggctgcc cagcacctga ccgccggttt
    19681 cgccttcagc aggcggcata cggaccatga tgtcgtggct gctaccaaag ttttgcagca
    19741 ttggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt tcagccggtt
    19801 tttcgagcgt aatttcgata accgtaccac cggtgaaatc cagcccccag ttaaagccgc
    19861 gcacgcccat aataacgata gcagcgatta acagcagacc agagatgccg aaagcccagt
    19921 agtcccagcg cataaagtca tagactttac ggccgtggtt tagttgttca acagtatatt
    19981 cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgcttgcc gccatatagc
    20041 aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc cacaccgata
    20101 ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag gatgatgact
    20161 ttaatcagcg tggtgatgtt cgcatcgaag atagaactga atgcgccacg ataaccttca
    20221 tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg ttcgttgatc
    20281 agtacgttcg catcgaccgc taccgcaagg gttaagacga tacccgcgat acctggcata
    20341 ctcagtgttg cgcctggcaa cagcgacata ataccgacga tcaagatcaa gttggcaatc
    20401 agagcactgg tcgcaatcag accaaacttc ttatagaaga tgatcatgaa cagaatagaa
    20461 accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat acccaaggtc
    20521 gggccaattg tacgttcttc aacaatctga atcggcgcga tcaacgcacc ggcacgcagc
    20581 agcagtgaca gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta
    20641 cccagacgag actggatgtt ggcgatgtta atcacctctt cctgtttcac cagaaccgca
    20701 cgaccatttg catctttctt accgctgtct ttgtactcca caaacagggt tgccatcggt
    20761 ttgccgatgt tgtccttggt gaagttagac ataatgttgc caccagcgct atcgagcgag
    20821 atgttaacct gcggctggtt gtattcgtcc tggctggaag tggagtcggt gatatggtca
    20881 ccagtcagaa ttacgcgttt gtacagcaca accggctgac cttcgcgggt ctgtttcact
    20941 tcagagtcgc ccggtacgcg accggatgcc gcagcggcct gatcaacatt ggtgtttacc
    21001 agacggaatt ccagcgttgc cgtcgcgccc agaatctctt tcgcacgcgc agtgtcctga
    21061 ataccgggca gttcaacaac gatacggtca gcaccctgac gctgaaccac cggctccgcc
    21121 acgccaagtt ggtttacacg gttacgcagg atattaatgt tctgctgcac cgcatattca
    21181 cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct gccctggctg
    21241 ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc acgagcttta
    21301 gcatcgcgga aagtgatgct caggccgtag ttgttttctt tacgaacagt ggtatacggg
    21361 atgccctttt cgcgcaggtc actgcgtagg ctatcgatat tttgttcctg gagtttgcca
    21421 agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg
    21481 agcttcatcg gctcagcatg aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta
    21541 agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc gcgcaactgg
    21601 gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac agacttagca
    21661 gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc actggcggcg
    21721 actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt gggaagcgca
    21781 tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa aggataacgg
    21841 tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat ggtgcctttc
    21901 ggcaggacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc attcagcgcg
    21961 atagcaatgt agccgttttc cgctactttg gttacgcgac caaccaggcc accgttcgtc
    22021 agaacttcat cacctttggc aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc
    22081 tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag catcaaaatc
    22141 aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc agaaataaaa
    22201 aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg gtacttctcg
    22261 cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt caatagcctt
    22321 gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc
    22381 gcctaatatt tcgttgcaac ggtcgagatg atgcaagtaa gcgcgtgaat aattgcgaca
    22441 ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatatt tcgcattgcg
    22501 gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc gggttggcat
    22561 tacgcagtca aacatatcga taccgcgacg tacgccttca accaggtctt ccggtttacc
    22621 aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc ggacacacat gctccagaat
    22681 acggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt agccatcaaa
    22741 gccgatatct accagacctt taacagatat atcacgtaaa tcttcgtaaa tactgccctg
    22801 aattatacca aataacgcat ttttgttacc aagactgtca aaacgctcac ggctacgctt
    22861 cgcccaacgc agagacatct ccatagagcg ttttgcgtaa tcccagtcag caggatacgg
    22921 cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa tctccatcga
    22981 cttttccggg tcgaggaaaa tcgggtcgcc gttgatcggg ttacggaagt gaacgccctg
    23041 ttcggtgatt ttacgaatat caccaaggct gaagacctgg aagccgccgg agtcggtaag
    23101 aatcggtcct ttccactgca taaaatcgtg cagatcgccg tgcagtttca tgatttcctg
    23161 gcccgggcgc agccacaggt ggaaggtgtt gccgaggata atttgcgcgc cagtggcttc
    23221 aacttcttcc ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca taaaacaagg
    23281 cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt
    23341 gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa cgtttaaacc
    23401 agcgccgcgg aattactccc cgacgcgctc attaattgcc tgcggattgt acgtgataaa
    23461 catcgcatca ccgtaactaa aaaagcgata tttctcttct accgccgctt tataggcatt
    23521 catggtgtgt tgataaccgg caaaggccga aaccagcata atcagcgtcg actctggcaa
    23581 gtggaagttc gtcaccagcg catcgaccac tttgtactgg aagcccggat agataaagat
    23641 ttgggtatcg tcaaagaacg gttcgataag atcgtttttt gccgcctgtg ccgcgctttc
    23701 cagcgaacgt actgaagtgg tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc
    23761 cagtaccgcg tctaccacat cctgcggtac ttcagcgtat tccgagtgca tgatgtgatc
    23821 ttcaatggtg tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa
    23881 cgccatctcc acgcctttgg cgcgtaattt ttccagcaaa ggctcgtcaa aatgcagacc
    23941 agcggtcggg gctgcaaccg cgcccggttt ttcgctataa acggtttgat aaagttcgcg
    24001 gtcagcgtct tcgtccggac gatcgatata cggcggcagc ggcatatggc cgatgctgtt
    24061 gaggatatcc agtaccgagc gttcatcatt aaattcgact tcaaacagtg cgccgtggcg
    24121 cgcggtcatt gttgcgttga tactttcgtc atcgcccagc aacagttctg cgccaggttt
    24181 tggcgctttc gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca tccgctcaac
    24241 cagcacttca atcttgccgc cgctggcttt acgcccaaac agacgcgccg ggatcacgcg
    24301 ggtattatta aaaaccagaa gatcgccggg gttgagctta tcgagtaaat cggtgaaagt
    24361 accgtgcgtc agcgcgcccg tcggcccgtc cagcgacagt aaacgacagc tactgcgttc
    24421 aggcatggga tagtgggcaa tcagggattc gggcaattca aaggagaaat cggtaacgcg
    24481 catgacactg actcagacta aaataagagt cgggtagtct agtgccgggg cgctctccct
    24541 gcaaccttta cccccctttg gatagaatat aatcgatgaa ttttttagct cacctgcatt
    24601 tagcccatct cgcggaaagc tcgctttctg gcaatttact ggctgatttc gtacgcggaa
    24661 atcctgaaga aagttttccg cccgacgtcg tggctggcat tcatatgcat cgacgtattg
    24721 actgacaatc tgccggaagt ccgcgaagca caggagtggt ttcgtagtga aacgcgccgc
    24781 gttgcgccta ttacgctgga tgtcatgtgg gatcactttc tttcccgcca ctggtcgcag
    24841 ctgtcgccgg actttccgct acaggaattt gtctgttatg cccgcgagca agtgatgacg
    24901 attttgccgg actcaccgcc acgttttatc aatctgaaca attacttgtg gtcagagcag
    24961 tggctggtgc gctatcgcga tatggatttc atccagaacg tgttaaacgg catggcaagc
    25021 cgccgcccac gtctggatgc cctgcgtgac tcctggtacg atttagacgc tcattatgac
    25081 gccctcgaaa cccgcttctg gcagttttat ccgcggatga tggcgcaggc gtcacacaag
    25141 gcgttataaa acgttagttc atccataccg tagccgaaat ggcaggcaaa gccagaatcc
    25201 cgtcagtcag ttgcccatgc ccttctttgc attgccattg cacggcattg agaaacggtg
    25261 acgcgggtag caccacttca ctggcctcgc cacggttgat tgccaccagt acacgttgct
    25321 gattcagcac gcggacaaat accactacgt tatcttccgc atacagcacc tgacagccgc
    25381 cacgacgcag cgcctgactt ttcttacgca gcacaatcat tcgccggtac agcgcgaata
    25441 acgccgtatc ctgcttttcc acctgccagg ggaacggttt acggcaaaac ggatcgtttt
    25501 taccatccag tcccacttca tcaccgtaat aaatgcacgg tacaccaggc caggtgaaca
    25561 gccagaccac cgccagcggc aggcgcgcaa tatcccgacc gagcagcgtt ttaaatcgcg
    25621 cagtatcgtg gctatcgagc tgattaaaca tacgtaattg ttgttgatga gaaagccctg
    25681 cgcggtaatt atccatccag gccatacagg tttgggcgtc aatttgctgc ggatcataag
    25741 agatgtcggt attggcaaga aatccccaca acgggaatgt gaagccacga tagttcatgg
    25801 cagcatcttc cacatcggcc tgtaaccatt gccgtgcatc gccaaaatgt tcgccgacaa
    25861 tatacgcttc cggctgggtt tctttcgccg cttcggtgat cccggcaacg tgctgcatat
    25921 tattgcgcgc cccacccgcc tcccccagca tatgcaccac atccagccgc cagccgtcca
    25981 tattccacgg cgctttcagc cagtggcgga caatactgtc ttccccgcga taaatttcat
    26041 tcaccagact ttccgactga taatccagct tcgtcaagct ggcatagcca agccagtcga
    26101 gcgccgtgcc atcatcacta aacgagtacc agtcgcgcca gggcgattca gagttgtgac
    26161 aagctccccc cgtgccaaga ttgtgcctgt caaaccaggc atgggaatcg ccactgtggt
    26221 taaacacgcc gtccagcacc agccgcattc ccagctgctg cgtattgtgt cgcaaacgca
    26281 gcaacacccc atcaccgcca aactgcggat cgacatggcg ataatcctcg gtatcgtatt
    26341 tatgtacgct gggagcttta aacaccggat tgagatacag cgctgtcacg ccaagctttt
    26401 tcagatacgg cagtttttcg cttatcccgt ccagatcgcc gccatagaac gttgatccgc
    26461 ccgcctgcgc cgtgaccggt tcatcccagt cacgcaagat gatctcttgt ccggctgcat
    26521 gatggtaata gacatgatcc tgttcagctt cacgaggaag actacgcgca aaacgatcag
    26581 ggaagatctg ataaaaaatc tgatccgcag cccattgtgg gccgatatca ggtacatcga
    26641 cggcaaactg ctccagtcgt gccggcggca ttcggctgaa gccctgcggt gtaaaccaac
    26701 gctggcgatc gtgccacagc agtttgaaac tgtaacgccg ccggggttgt ccgctggaga
    26761 gatcaatcgc cgcacgccat gcggtgacgc ctggctgcgg ctgactgcgc tgcttatgca
    26821 ttggtactga catttcttcg ttatcgtgtt ctgtacgcag cataatgcgc tgcggtgggt
    26881 cttcgcccgt cagccacagt gtaatgagca gttgatcttt gctttgttta acaaatgggg
    26941 gcaccggcag gtgccatgca tttaacatca taattcccct gtgatgaaaa tctgatcacc
    27001 ttgccatagc ctaatgcagg atcactcatc atctgaagga tatttggggg aggaggacgg
    27061 gggcggataa ctttcggaat agccggatca ggatttcctc ctgaaccggc ttacagaacg
    27121 caagtattat tggttttcag ccagctgacg atcgtggcgg cgtttaaaca tccagccaat
    27181 caacagcaca acaatccacg cgaaaccgac atacagcgag atacgcgtat ccgggtgata
    27241 accaatcaac ccgataataa agagcaggaa aatcagcccg ccgatggtcg ttgctacccc
    27301 acccggcact ttaaatttca gcgccttaac ttcttctggc ggcaaacggc gacggaaggc
    27361 gatttgcgac agcaggatca taatccacac ccataccgtg gcgaaggttg ccagcgaagc
    27421 gatcaccagg aagacgtttt ccggcatgat gtagttcaga tacaccgcaa acagcagcgc
    27481 ggtagtcatc accagcaccg taacccacgg aataccgcga cgcgacgttt tgctgaaaat
    27541 tttcggtgcg ctgccctgct ctgccatacc gtggagcata cggcctacgc caaatacgtc
    27601 actgttaatt gccgacagcg aagcggtcag cacaacaaag ttaagaatgc tggcggcaaa
    27661 ggtaatgccc atatgctgga acgtcagtac gaacgggcta ccggcagtgc caacctgatt
    27721 ccacgggtag atagacataa tgacgaacag cgtaccgacg tagaacacca gaatacgcat
    27781 cggcacggag ttaatcgcac gcggaatcga tttctcagga tctttcgctt caccggcggt
    27841 aatcccgata atttcgatcc caccgtaagc aaacatcacc atttgcaacg acattaccat
    27901 gccaagccag ccgttactga agaagccgcc gttgctccac aggttatgaa taccggtcgg
    27961 ttgcccgccg ttgccaatcc cccagatgat gatgccgaaa ccggcgacaa tcatgatgat
    28021 gatggtggcg actttaaaga acgagaacca gaattccagc tcaccgaaga ccttcacgct
    28081 catcaggttt acggcgcaaa tgatcagcac cacgctcagt acccaaatcc agtgcggcac
    28141 cgtcgggaac aagacaccca tatagatacc aaaagcggtc acatcggcga tggcgacaat
    28201 aaggatttca aaacagtagg tccatccggt aatgtaacct gccagcgggc caaggttttc
    28261 ctgcgcataa cgcgagaaag agctggcggc cgggttatgt accgacattt cccccagcgc
    28321 acgcataatg atatacgccg cgataccacc gataatatag gccaacaaca cgctcggacc
    28381 ggccattttg atggcgtctg ccgaaccgta aaacagcccg gtgccaattg ctgaacccag
    28441 tgccataaag cgtatgtggc gggtacttag cccacgcttt agcttattct tactttccat
    28501 caattcctaa cccatcaaca caataaaaaa ccacggggca tcgcagcccc gtggttaaaa
    28561 caaatgttca gtgatttagt gagcgctgga ggtcacctga cgacctgccg cgcgatccca
    28621 gataatggcc agaaccacca tcaccactgt tggcattaac cacgccagac cttgttcggc
    28681 cagcggtaaa cgctgcgccc aggacggtaa gatatcgctg aatgcagatg ctttgatccc
    28741 gtcgagaata ccaaaaagca ggctgataaa catcggcgga gcaatcacgc gggacgaatt
    28801 atgccaccat gagcgtgtaa aacttaatac aaccagtgcg atacacggcg gataaatagc
    28861 ggtcagcacc ggtacggaga tctggatcag ctggcttaag ccgaggttag acaccaccat
    28921 cgagaagccg ccgaggataa acaccagcgt acgataagag agcggtacgt actgggcaaa
    28981 gaattctgca caagcacagg tcaggccaac cgccgtgacc agacaggcga tgaagattaa
    29041 cgccgccagc aggaagctac cgccgccgcc aaaggtgtgc tgaacgtaag catgcagaat
    29101 agcagcgccg tttgcagact gatcgaccag cgacgcgctg tctgaaccca gacagaacag
    29161 cgccaggtac agcaaagtca gaccaacacc cgccatcagg ccagcccaga cggtataacg
    29221 ggtcagcaga cgcgcttcgg taacgccacg agaacgcgcc gcgttaacaa taacgatacc
    29281 aaacaccatt gcgcccagcg tatccatggt cagatagccg ttaacgaagc cgttagaaaa
    29341 tgcagcgttt tgataagcct cagtcgccgt gctgatagaa cccgccggcc agacaatcgc
    29401 gacaacagac aggatgacca gcgcgataat tttcagcggc gcaaggaagt tgcccacggt
    29461 atccagcagc ttgcccggat agagcgaaac cagaataacg atagcgaaat agaccaggct
    29521 gtagataaac agcggcagcg cggaatcacc cgtcagcggc gcaatcccca cttcaaagga
    29581 aacggtagct gtacgcggcg tagcgaaaag cggccccacc gccaggtaac aaaccgttgc
    29641 cagcagtacg ccagcgactt taccgattgg cgtgctgagg ctgtcaacac cgccgccaac
    29701 ttttgccagc gccactaccg ttaataccgg caggccaacg gcagtaatga ggaagccgaa
    29761 tgccgcagtc cagacgtgtt cgcctgcctg taagccgacc attggaggga aaataatgtt
    29821 acctgcgccg acgaacaacg caaatgtcat aaagcccaga gcgatgatat cgcgcgatct
    29881 taattgatgg gtcataaatc atactgcctg tggatgtggt gttgtaacgt taaatttttg
    29941 tgccaatccc tggggatgat acagcggtat atttaaacaa ttttagcggg aaatactccc
    30001 aaccaggcgc ggcgaagaat agtttttcga tttaccgcgt taatgcagtc agtatgacag
    30061 cacctgaagc gcaatttaaa cgcttataac gttttaaagc aaggagggat ctaaaaacca
    30121 gaggtatata gcgataagaa atcaccaacc agtataaaac actatttccg tgaaaaagac
    30181 atggctaatc atgcgaacaa aaaatcacca accgttgtaa aatctaacgc ccgtattttg
    30241 actgcaaaga aaataagcca gcatcggctg gcttatggca ataaaagatg acaaaggcgg
    30301 attaatcgct gtttttggca attaaacgtt ccgggataac aaaactgaaa cgtgttcctt
    30361 ttcctactgt actctcaata ttcaggcgac tttcgtgatg attcaccgca tgtttcacga
    30421 tcgctaaccc taatccgcta ccgccggttt gccgggaacg cgctttatca acgcgataaa
    30481 aacgctcggt caggcgcgga atatgctccg gtgcaatgcc cggtccgtta tcttcaacgc
    30541 taaattcggc accgtgcggc actcgctgcc agcgtacggt gatatgcgtg ccttccggcg
    30601 tatgattcac ggcgttatag accaggttcg aaatcgcact gcgtagctga tcttcgttgc
    30661 cagacacctt gaggccgtta tctatctcaa aggtaaatgt ctgttttttc tgactcagag
    30721 tctgagcctc gcgctcaaca acgcgcagca tcatcggcac atcaaccttt tcattgagca
    30781 attgcgtcgg cgcggcttca attttcgaca gcgttagcaa ctgcttcacc agcccttcca
    30841 tccgctgggt ctgctcgcgc atggtgtgca acgctttttc gcgtaccgcg ccttccagcg
    30901 gctgctcatc catcatctcc aggtaaccct gtaacacggt caatggcgta cgtaactcat
    30961 ggctcacgtt ggcaaaaaag ttacgccgcg ccccttccag ttgatgcatt tgcgtgacat
    31021 cacgcgccac catcagcaac tgtttgtggg tataaggcat gacgcgaatt tccagatgcc
    31081 gcccggtgtt gagcaccaga ttgagcgggc gagaaaaatc acgcgttttc agatattgcg
    31141 taaactccgg gtaacgcagt aggttaagga tgttctgccc gttatcttcc ggccagcgca
    31201 aaccaagaat ttgttgcgcc agaccgttac accagaaaat accgccctct tccgtggtca
    31261 gcaccaccgc gtcgggcagc gactccgcgc cgctacgaaa gcgtttaatc agattgccca
    31321 gttcacggcg gcgtttttta tttcgcagct gcatctggtg taagccgtat agtagcggtt
    31381 cccagctacc acgccccggt ggcggggtca tactgcgatc cacccacagc caccatgaaa
    31441 ggcgcaataa attccagaaa tgccagataa gcagtcctgt taccgatgcc agcaaaaacc
    31501 agggcaggta accaaaaaat gcacccagga tgaaagccgg gaggcagcaa agtagcagct
    31561 ccagcaccag ccttttccac gacagccgtt ccagcacgta agatactcca gttaagaaat
    31621 cataagccct gctctgcgtc cgatgagcaa ggcgttaaaa gcgggttgaa aaacgatatc
    31681 ctgtaccgcg cacggtctgc accatgcggt catgcccgcc gggctccagt gctttacgca
    31741 ggcgacgaat gtggacatcg accgtgcggt cttccacata cacgttagtt ccccagacgt
    31801 ggtttaacag ctgctcgcgg ctgtacacgc gctcaggatg cgtcataaaa aagtgcagca
    31861 gtttaaattc tgtcggcccc atctccagcg gctcttcgcc cgccatcact cggtgagatg
    31921 tcgggtcgag acttaatccc tgcatcttaa tcacctcttc caccgccatt ggcgaaatac
    31981 ggcgcattac cgctttgatt cgcgccacca gctccttcgg cgaaaatggc ttggtgatat
    32041 agtcatccgc gccggtttca aggccgcgca cgcgatcttc ttcttcccct ctggcggtca
    32101 acatcaccac tggaatatcc cgggtcatcg actcgcgctt gaggtgtttg atgaactgga
    32161 taccggagcc gccaggtaac atccagtcaa ggagaattaa atccggctag ggttcattca
    32221 gttgattcac agcactgtca taatcttccg cttcgaccgg ctgaaagcca ttttgttcga
    32281 gcacgaagca gaccatttcg cgaattggag cttcatcttc tacgaccaga atacgtctcg
    32341 ccatgatttg ccctgttgta ataaataggt tgcgatcatt aatgcgacgt cattatgcgt
    32401 cagatttatg acagatttat gaaaagctcg tcgcacatat cttcaggtta ttgatttccg
    32461 tggcgcagaa aaaagcaaat ggcacatctg tttgggtata atcgcgacca tgctttttcg
    32521 ccagggaacc gttatgcgca tccttcacac ctcagactgg catctcggcc agaacttcta
    32581 cagtaaaagc cgcgaagctg aacatcaggc ttttcttgac tggctgctgg agacagcaca
    32641 aacccatcag atggatgcga ttattgttgc tggtgatgtt ttcgataccg gctcgccgcc
    32701 cagttacgcc cgcacgttat acaaccgttt tgttgtcaat ttacagcaaa ctggctgtca
    32761 tctggtggta ctggcaggaa accatgactc ggtcgccacg ctgaatgaat cgcgcgatat
    32821 catggcgttc ctcaatacaa ccgtggtcgc cagcgccgga catgcgccgc aaatcttgcc
    32881 tcgtcgcgac gggacgccag gcgcagtgct gtgccccatt ccgtttttac gcccgcgtga
    32941 cattatttcc agccaggcgg ggcttaacgg tattgaaaaa cagcagcatt tactggcagc
    33001 gattaccgat tattaccaac aacactatgc cgatgcctgc aaactgcgcg gcgatcagcc
    33061 tctgcccatc atcgccacgg gacatttaac gaccgtgggt tccagtaaaa gtgacgccgt
    33121 gcgtgacatt tatattggca cgctggacgc gtttccggca caaaactttc caccagccga
    33181 ctacatcgcg ctcgggcata ttcaccgcgc acagattatt ggcggcatgg aacatgttcg
    33241 ctattgcggt tcccccattc cactgagttt tgatgaatgc ggtaagagta aatatgtcca
    33301 tctggtgaca ttttcaaacg gcaaattaga gagcgtggaa aacctgaacg taccggtaac
    33361 tcaacccatg gcggtgctga aaggcgatct ggcgtcgatt accgcacagc tggaacagtg
    33421 gcgcgatgta tcgcaggagc cacctgtctg gctggatatc gaaatcacta ctgatgagta
    33481 tctgcatgat attcagcgca aaatccaggc attaaccgaa tcattgcctg tcgaagtatt
    33541 gctggtacgt cggagtcgtg aacagcgcga gcgtgtgtta gccagccaac agcgtgaaac
    33601 cctcagcgaa ctcagcgtcg aagaggtgtt caatcgccgt ctggcactgg aagaactgga
    33661 tgaatcgcag cagcaacgtc tgcagcatct tttcaccacg acgttgcgta ccctcgccgg
    33721 agaacacgaa gcatgaaaat tctcagcctg cgcctgaaaa acctgaactc attaaaaggc
    33781 gaatggaaga ttgatttcac ccgcgagccg ttcgccagca acgggctgtt tgccattacc
    33841 ggcccaaccg gtgcggggaa aaccaccctg ctggacgcca tttgtctggc gctgtatcac
    33901 gaaactccgc gtctctctaa cgtttcacaa tcgcaaaatg atctcatgac ccgcgatacc
    33961 gctgaatgtc tggcggaggt ggagtttgaa gtgaaaggtg aagcgtatcg cgcgttctgg
    34021 agccagaatc gggcgcgtaa ccagccggac ggtaatttgc aggtgcctcg cgtagagctg
    34081 gcgcgctgcg ccgacggcaa aattctcgcc gacaaagtga aagataagct ggaactgaca
    34141 gcgacgttaa ccgggctgga ttacgggcgc ttcacccgtt cgatgctgct ttcgcagggg
    34201 caatttgctg ccttcctgaa tgccaaaccc aaagaacgcg cggaattgct cgaggagtta
    34261 accggcactg aaatctacgg gcaaatctcg gcgatggttt ttgagcagca caaatcggcc
    34321 cgcactgagc tggagaagct gcaagcgcag gccagcggcg tcacgttgct cacgccggaa
    34381 caagtgcaat cgctgacagc gagtttgcag gtacttactg acgaagaaaa acagttaatt
    34441 accgcgcagc agcaagaaca acaatcgcta aactggttaa cgcgtcagga cgaattgcag
    34501 caagaagcca gccgccgtca gcaggccttg caacaggcgt tagccgaaga agaaaaagcg
    34561 caacctcaac tggcggcgct tagtctggca caaccggcac gaaatcttcg tccacactgg
    34621 gaacgcatcg cagaacacag cgcggcgctg gcgcatattc gccagcagat tgaagaagta
    34681 aatactcgct tacagagcac aatggcgctt cgcgcgagca ttcgccacca cgcggcgaag
    34741 cagtcagcag aattacagca gcagcaacaa agcctgaata cctggttaca ggaacacgac
    34801 cgcttccgtc agtggaacaa cgaactggcg ggttggcgtg cgcagttctc ccaacaaacc
    34861 agcgatcgcg agcatctgcg gcaatggcag caacagttaa cccatgctga gcaaaaactt
    34921 aatgcgcttg cggcgatcac gttgacgtta accgccgatg aagttgctac cgccctggcg
    34981 caacatgccg agcaacgccc actgcgtcag cgcctggtcg cgctgcatgg gcagattgtt
    35041 ccccaacaaa aacgtctggc gcagttaatc gtcactatcc agaatgtcac tctggaacag
    35101 acgcaacgta atgtcgcact taacgaaatg cgccagcgtt ataaagaaaa gacgcagcaa
    35161 cttgccgatg tgaaaaccat ttgcgagcag gaagcgcgca tcaaaacgct ggaagcccag
    35221 cgtgcacagt tacaggcggg tcagccttgt ccactttgtg gttccaccag ccacccggcg
    35281 gtcgaggcgt atcaggcgct ggagcctggt gttaatcagt ctcgattact ggcgctggaa
    35341 aacgaagtta aaaagctcgg cgaagaaggt gcggcgctgc gcgggcaact ggatgcatta
    35401 acgaagcagc ttcagcgcga tgaaaacgaa gcgcaaagcc tccgacaaga tgagcaagca
    35461 cttactcaac aatggcaagc cgtcacggcc agcctcaata tcaccttgca gccacaggac
    35521 gatattcaac cgtggctgga tgcacaagat gagcacaaac gccagctgcg gttactcagc
    35581 caacgacatg aattacaagg gcagattgcc gcgcataatc agcaaattat ccagtatcaa
    35641 cagcaaattg aacaacgtca gcagcagctc ttaacggcat tggcgggtta tgcactgaca
    35701 ttgccacagg aagatgaaga agagagctgg ttggcgacac gtcagcaaga agcgcagagc
    35761 tggcagcaac gccagaacga attaaccgcg ctgcaaaacc gtattcagca gctgacaccg
    35821 attctggaaa cgttgccgca aagtgatgat ctcccgcaca gcgaagaaac tgtggcgctg
    35881 gataactggc ggcaggttca tgaacaatgt ctcgcattac acagccagca gcagacgtta
    35941 cagcaacagg atgttctggc ggcgcaaagt ctgcaaaaag cccaagcgca gtttgacacc
    36001 gcgctacagg ccagcgtctt tgacgatcag caggcgttcc ttgcggcgct aatggatgaa
    36061 caaacactaa cgcagctgga acagctcaag cagaatctgg aaaaccagcg ccgtcaggcg
    36121 caaacgctgg tcactcagac agcagaaacg ctggcacagc atcaacaaca ccgacctgac
    36181 gggttggctc tcactgtgac ggtggagcag attcagcaag agttagcgca aactcaccaa
    36241 aagttgcgtg aaaacaccac gagtcaaggc gagattcgcc agcagctgaa gcaggatgca
    36301 gataaccgtc agcaacaaca aaccttaatg cagcaaattg ctcaaatgac gcagcaggtt
    36361 gaggactggg gatatctgaa ttcgctaata ggttccaaag agggcgataa attccgcaag
    36421 tttgcccagg ggctgacgct ggataattta gtccatctcg ctaatcagca acttacccgg
    36481 ctgcacgggc gctatctgtt acagcgcaaa gccagcgagg cgctggaagt cgaggttgtt
    36541 gatacctggc aggcagatgc ggtacgcgat acccgtaccc tttccggcgg cgaaagtttc
    36601 ctcgttagtc tggcgctggc gctggcgctt tcggatctgg tcagccataa aacacgtatt
    36661 gactcgctgt tccttgatga aggttttggc acgctggata gcgaaacgct ggataccgcc
    36721 cttgatgcgc tggatgccct gaacgccagt ggcaaaacca tcggtgtgat tagccacgta
    36781 gaagcgatga aagagcgtat tccggtgcag atcaaagtga aaaagatcaa cggcctgggc
    36841 tacagcaaac tggaaagtac gtttgcagtg aaataactat tcagcaggat aatgaataca
    36901 gaggggtgaa ttatctcttg gccttgctgg tcgttatcct gcaagctatc actttattgg
    36961 ctacggtgat tggtagccgt tctggtggtt gtgatggtgg tatgaaaaaa gtcattttat
    37021 ctttggctct gggcacgttt ggtttgggga tggccgaatt tggcattatg agcgtgctca
    37081 cggagctggc gcataacgta ggaatttcga ttcctgctgc cgggcatatg atctcgtatt
    37141 atgcactggt ggtggtggtc ggtgcgccaa tcatcgcact cttttccagc cgctactcac
    37201 tcaaacatat cttgttgttt ctggtggcgt tgtgcgtcat tggcaacgcc atgttcacgc
    37261 tctcttcgtc ttacctgatg ctcgccattg gtcggctggt atccggcttt ccgcatggcg
    37321 cattttttgg cgtcggagcg atcgtgttat caaaaattat caaacccgga aaagtcaccg
    37381 ccgccgtggc ggggatggtt tccgggatga cagtcgccaa tttgctgggc attccgctgg
    37441 gaacgtattt aagtcaggaa tgctggcgtt acaccttttt attgatcgct gtttttaata
    37501 ttgcggtgat ggcatcggtc tatttttggg tgccagatat tcgcgacgag gcgaaaggaa
    37561 atctgcgcga acaatttcac tttttgcgca gcccggcccc gtggttaatt ttcgccgcca
    37621 cgatgtttgg caacgcaggt gtgtttgcct ggttcagcta cgtaaagcca tacatgatgt
    37681 ttatttccgg tttttcggaa acggcgatga cctttattat gatgttagtt gggctaggga
    37741 tggtgctggg aaatatgcta agtggcagga tttcaggacg ttattcacca ctgcgcattg
    37801 cagcagtgac tgactttatc attgtactgg cactgctgat gctctttttc tgcggcggca
    37861 tgaaaacaac gtcgcttatt tttgctttta tttgttgcgc gggattattt gccctttcag
    37921 cacctctgca aatattgtta ctgcaaaatg ccaaaggagg agagttatta ggtggcgcag
    37981 gtgggcaaat agcgtttaac ctcggtagcg ccgtcggcgc atattgcgga ggtatgatgc
    38041 tgacgctggg gctggcatat aattacgtgg cgctgcctgc cgccctgctt tcgtttgctg
    38101 cgatgtcatc gttgctgctg tatggtcgct ataagcgcca gcaagcggcg gatagtccgg
    38161 tgctggcgaa accactgggg tagattactg tcacggtgcg cgttgcttat tgccggatgc
    38221 ggcgtgaacg ccttatccgc cctacgcggt tctggcacat tttgtaggcc tgataagacg
    38281 cggcaagcgt cgcatcaggc atgatgcgcc aattgcctac gttttttact cttgtggcca
    38341 taaccacgca gcgccgcgta cgccgctgga atcaccgtgc ttcgccttac tcaccggcgt
    38401 ttcacattcg ccgccgaaga caaattgttt aatcaactgc ccaaccgttt gatataaacg
    38461 gtctacattg ctcatcccgc cccccaggac aatcacatcc ggatcgagaa tattcacgac
    38521 atgtgccagc gattttgcca gccgcagctc gtagcgacgc aatgccagtt ccgctaccgg
    38581 atcgctttct tcaaccaggc ggataatttc actgcctttc agcgcatgtc cgctcaaacg
    38641 acgataatcc gtcgcgaatc ccgtgcccga aataaaggtt tcaatacaac cttgtttacc
    38701 gcaataacaa gggacttcct cgcgataacg cagttcgtct tcgtccatcc acggtagcgg
    38761 attgtgtccc cactcacctg ccgtgccatt gccgccgata tgcgtccgcc cattgaatgc
    38821 cacgcccgcg ccgcatcccg tgccgatagt cacggcaaat accgtctgcg ctcccgctgc
    38881 cgcgccatct actgcttctg aaaccgccag acagttggca tcatttgcca gccgcacttc
    38941 ccgctgcaac ctcgcgctta agtctttatc gaatggctga ccgttgagcc aggttgaatt
    39001 gacattcttc accacaccgg tgtagggcga aatcgaacct ggaatgccca tacctaccgt
    39061 tccgcgctgc cctgtcgcct gctccgccat atcaaccaac gtggcgatcg tttcaatagt
    39121 ctgccggtaa tcatcacgcg gcgtgggcag acgatggcgg tacaactgct cccctgcatc
    39181 gcccagtgca atcacttcag ttttggtgcc gcctaaatcg atacctatac gcactgtact
    39241 ctccttattt ttttcaatat caatagcgta gagacggaca accggattgg caatgcaagg
    39301 ccgccgacaa ttcgttatca tgcccgctaa atttaacgac aaggccgtgg aaattatcat
    39361 gctgtggttc aaaaatttaa tggtttaccg tcttagccgc gagatttcgc tgcgtgcaga
    39421 agagatggaa aaacagctag cctcgatggc atttacccca tgcggcagcc aggacatggc
    39481 gaagatgggc tgggttcctc cgatgggatc gcacagcgat gcgttaacgc acgttgccaa
    39541 tggtcaaatt gttatctgcg cgcgcaaaga agaaaaaatc ctcccgtctc cggtgattaa
    39601 acaggcgctg gaagcgaaaa tcgccaaact ggaagcggaa caggcgcgta agctgaagaa
    39661 aaccgaaaaa gattcgctga aagacgaagt gctgcactct ctgctgccgc gtgctttcag
    39721 ccgttttagc cagacaatga tgtggctcga cacggttaac ggtttgatta tggtggactg
    39781 cgccagtgcc aaaaaagcgg aagatacgct ggcattactg cgtaaaagcc tggggtcgtt
    39841 accggttgta ccattgagca tggaaaaccc gattgaactg acgctgaccg aatgggttcg
    39901 ctccggtagt gcggcacagg gcttccagct gcttgatgaa gccgagctga aatcgttgct
    39961 ggaagatggc ggcgtgatcc gcgcgaagaa acaagatctg accagcgaag agatcaccaa
    40021 tcacattgaa gccggaaaag tggtgactaa actggcgctc gactggcagc agcgcattca
    40081 gtttgtgatg tgcgacgatg gttcgctcaa gcgtcttaag ttctgcgacg agctgcgcga
    40141 tcaaaacgaa gatatcgacc gtgaagattt cgcccagcgt tttgatgccg atttcatcct
    40201 gatgactggt gaactggcag cgttaattca aaacctgatt gaaggattag gtggcgaagc
    40261 acaacgttaa ttgctgattt tcctttaatg ccggatgcga cgcctgccgc gtcttatccg
    40321 gcgtacgaag ccacaccaag catataatta ttcgctacgg cgagcaataa tttttagcgc
    40381 agcaatatta tgcgttttac gccgtaactg atctaatcgc ctcatcccta ttcgcctgtc
    40441 agcagcacgc agaatattca ctaaccccca tttatcgctg gtaaattgtt ttgttaacag
    40501 ctccgctttt ggggtatcaa tatattcacg caataaatca gaaattgaac aaatatccgt
    40561 caggtaagga taccagcaag tttcaccgtg aaaattacgc actccagcgt tacctgcaat
    40621 aaagtctttc gggtaatccc agatcacgtt tttatccaac gtgatccaat aacgcggtaa
    40681 atcggtactt ccaccatttt ggctgcgcat gggataacgg gtacagtgaa tttgcaggtt
    40741 gatcgttggc gtcagtaagt catagatctc ccgctgtaac ttgctccatg gacgttgtgt
    40801 cattgttttt cctcaagccg gtgacgcgct tcactgcgaa ttttccgcgt tgccccggtt
    40861 ttcactaaat cacgtaactg cgaaagatcg ggttattcga caaataagag taatgatgga
    40921 tcctctttta accacgccgt tttcacatcg gcagccagct gtggattatt aatagccagc
    40981 gaccgatcct gaggctcagt caatgtcgtt aacaacgacg ccggggtatg tgcgttgcgt
    41041 aatgccgttt ggcgcaaatg atcattgcga ctttgcagca atgcacgcca ccattcatca
    41101 tctgatttat cttttatcgt agccagttcc gcaaaaaata cctggagatc ttcactgata
    41161 tttatatcaa ccttgatgaa ttatgaatat atcggaaatt gaaaaaatcg cagcaaagga
    41221 aatattatta tgggttaaag agaggatatt tcagaaaaat gccctcccgt tcaaggggaa
    41281 ggcgaggaat tacagatagc ggcacagata agaggtaggt tcggcaactt gaagatgaaa
    41341 ctcactgtga ccgggaacat taaacaccga accggcttca tacacttgcc agtcggtcgc
    41401 gtcaggcagt aacacattca gcgcgccact gattaccgtc atctcttccg gctcagcggt
    41461 gctgaaggtg tattcgcctt caaccataac acccacgctg gcgcgaccag tgctgctgct
    41521 ggaaaagccg atcgatttca ctttgccgga aaagtactca ttactttgaa gcataaactg
    41581 gccctgtctg gtagttgaac atctacttca ctataggggc cagaggcgct ggctgtcacg
    41641 caaaattaca ccagtaattc cgcagccagc cgtgcaatca atacgttaga gagcaagaca
    41701 ggaacatcga gctgtttttg cagtaaatcg cgatgacgtt ggtgaaatcc taaacaatcc
    41761 agcatgatga catcagcccc ttttgccagt aattctttcc cagcatcaat gattttttgt
    41821 tctgaatcat gaatggggtt acccaatgaa aataccggcg atttctgcaa aatttgccat
    41881 ttttgcgcct gaacgggcag catctcctca accggaacga taacccccac ctgatgatct
    41941 tcaacaatag aggaaaccag tggaggcaat attcgcgacg gctcaagaaa gatcgtatta
    42001 cgcgcagtca tactactaat gtttgctgta ctcattaata aaatgacgtc ataaccctga
    42061 ttatcgagca cttcaaccac accttgcaga tctcgctcca ctttacgacg cgaaacatgg
    42121 gccagctggt tgtcatttaa taatgtgaga atggtgtctt cgcctgcttc tggcgcgtac
    42181 tccgccatca cttcttcacg acttaacttc cccagcaggc tatgatggga aatattatct
    42241 tcgtcaatgt attccgtcag gagcggcaaa acctcctgca taggtacaat gccgatggtc
    42301 aggatcgcca acgacgcact cattgttcat ccaccttact tttctttcgt cgttaattac
    42361 cgggcaagtg tgaagcacca ctgctgacac ttacttgtgc tacaaatgac aaaaagcgta
    42421 gcagcagacg tgcacggcat aatgtaaaga ttccaaatga ttccagtaat ggaatgttat
    42481 tgtttaatat tctaattatt agaaaaacat aaattatgaa aaatgtgacg cagatccctt
    42541 gtagtggcac tcagaatccc ttccgagcag tctgatgcgg tcactatttg tcagggtcct
    42601 catagcgttt tttcgcatcc atcgcttcct gagccgtcgg atgttcactg atcaacgagt
    42661 ctggtttagg atgatcggct ctgagttggt accaggttac cgtctgtcct ggctttcctt
    42721 tttcaatcgt cactatatat gcttcacgag gataaggcgg tttcgttggc atcgttcttc
    42781 cttatttcac gggatgaacg ttaagtatag gcgctctaaa atcaacaatt gatcgtctgt
    42841 gccagggcgc tacgaatttc agaaatcacc tggctgggtt cgtttgttgc gtcgatgata
    42901 atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca gcacttcctg aacttcttcg
    42961 ctcagcggtt ttcccgttaa ggttggccgt aaatcttctt ccggtgcagc ttgcagtcgg
    43021 ttaaccagga ctgatactgg cgcacacaaa taaaccacga tcccgttatt ttgcatgaag
    43081 tgacgattaa attccgtcag aataatgccg ccgcctgtag cgataacggt ggatgccgca
    43141 gttaccgctt ccagcgccgc cgtttctctg gcgcgaaatc ccgcccactc ttccctttcg
    43201 acgatctccg cgaccgtcat attgagctgt gattgcaacc actgatcggt atcgacaaac
    43261 cgacggttaa gcgaatcggc aagggccatt ccgaccgttg ttttaccaca gccccgaggc
    43321 ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc aataggtcgc aatgcggcga
    43381 aagccggtgt catgagaata gcgatcatac catcaaacta gtacaatttc gattgtaaag
    43441 aaaaaattcc acttaaagta aaaatctcaa tacatccctt actataccaa taaatattca
    43501 agaatgaagt gtaaataata aattacattt agccacgact acgttgcact tccagccacc
    43561 acttctccag ctccgcggca aaggcctggc ggtcacgttg tgaaaggcta tctggtccgc
    43621 cggtctggat cccactggca cgtaaggtat ccataaaatc gcgcatcgtc aggcgctcac
    43681 gaatggtcgc tggcgtgtaa cgttcgccgc gcgaattaag cgccgctgcg cctttctcga
    43741 tggcttcagc agccaaaggt atatctgcgg tgatcaccaa atcgcccgct tcacactgcc
    43801 ggacaatttc gttatcggca acgtcgaaac ctgccgcgac gcgcagcgta cgaataaatc
    43861 gcgatggcgg cacgcgtaaa ctctggtttg ctaccagtac cagcggcatc tgcatacgtt
    43921 ccgccgcgag atacaaaatc tctttaatta cattgggaca cgcgtcggca tccacccaaa
    43981 ttgtcataaa gtcatccttt gttaggtaat cctctattgt gtcgcgcttt tgccttccgg
    44041 catagttctg tttatgcttc tgccagcgat tatcaaaaca atgaatttca cggcaggagt
    44101 gaggcaatgg aaaagaaaat cggttttatt ggctgcggca atatgggaaa agccattctc
    44161 ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa tctgggtata caccccctcc
    44221 ccggataaag tcgccgccct gcatgaccag ttcggcatca acgccgcaga atcggcgcaa
    44281 gaagtggcgc aaatcgccga catcattttt gctgccgtta aacctggcat catgattaaa
    44341 gtgcttagcg aaatcacctc cagcctgaat aaagactctc tggtcgtttc tattgctgca
    44401 ggtgtcacgc tcgaccagct tgcccgcgcg ctgggccatg accggaaaat tatccgcgcc
    44461 atgccgaaca ctcccgcgct ggttaatgcc gggatgacct ccgtaacgcc aaacgcgctg
    44521 gtaaccccag aagataccgc tgatgtgctg aatattttcc gctgctttgg cgaagcggaa
    44581 gtaattgctg agccgatgat ccacccggtg gtcggtgtga gcggttcttc gccagcctac
    44641 gtatttatgt ttatcgaagc gatggccgac gccgccgtgc tgggcgggat gccacgcgcc
    44701 caggcgtata aatttgccgc tcaggcggta atgggttccg caaaaatggt gctggaaacg
    44761 ggagaacatc cgggggcact gaaagatatg gtctgctcac cgggaggcac caccattgaa
    44821 gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag tgatcgaagc gatgacgaag
    44881 tgtatggaaa aatcagaaaa actcagcaaa tcctgatgac tttcgccgga cgtcaggccg
    44941 ccacttcggt gcggttacgt ccggctttct ttgctttgta aagcgccaaa tctgccgatt
    45001 tcaaccactc acgatagtga ctcatttgtg ggttcagcgg cgcaaccccc acactaatcc
    45061 gtaaagttac ctgtggcgta ttcggcaagc gtaacgtgtt tagcccttca cgcacccgta
    45121 acatggcggt aatggcgctc tcagctggcg taccggacat gattactgca aactcatcgc
    45181 cgccaaaccg accaatcaca tcgctaccgc gcagggtaat ttgtaactgt cgggtaagcg
    45241 ccacaatcgc ttcatcgccc acatcatgac cccaggtatc gttgatgctc ttgaaatggt
    45301 cgatatcgat aatcagtaac gttgcatcgc gattatgccg ccgacagtta tcaaattcat
    45361 tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc cgtcatgccg tcgcgagtac
    45421 tcatgacctg caacctgcgt ttatgttccg ccagtttggt tgccgtctgg tagctgaccc
    45481 agccaaacat cagaggataa acgacaataa tgggaaggga gagccaccat tccagcggag
    45541 cactattgaa cgacacggta atgcccgtaa gctcgagggt gacaaggcag gaaaccacca
    45601 tcaacaccag acccgcgaca aacagacggg ggccgcctgc ccccatcaaa ttcagacaca
    45661 gaatcatcaa catagcggtg gaaggcagca cgtttacgcc cattacgcct acccacattc
    45721 ccgctaatac tgcatcggtt tttaagttgt aaatttcccg gctaagcgga tcgacggccc
    45781 tgctcgctat ctgccaggct aaatgcggcc agacgaacgc ccagccgacc aacaccagcc
    45841 accaccagcc cggcggcggg tgcgaaacca gcgttgaagc aatcggtaag aacatgccag
    45901 ccaggccaac cgcacggggt agtctgacgc gacgggcgaa gcgcagcccg gaccgctgat
    45961 gttcgttttg aggagttaaa ggaggttcct ccccatgcgc cgccgctttt ttgaaaaagt
    46021 tttcatcatt cattattttt gggaacattc agaaacaatt ttcccaaatt atagagacgg
    46081 ggctaaaggc agaagtatga aatttcgggt gagccacttt cgtagctcac ccgttagcac
    46141 tat
//
LOCUS       DAEEDS010000029        46118 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEEDS010000029 DAEEDS010000000
VERSION     DAEEDS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.029"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(19..606)
                     /gene="mog"
                     /locus_tag="KLH32_001679"
     CDS             complement(19..606)
                     /gene="mog"
                     /locus_tag="KLH32_001679"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBE0138545.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(721..1674)
                     /gene="tal"
                     /locus_tag="KLH32_001680"
     CDS             complement(721..1674)
                     /gene="tal"
                     /locus_tag="KLH32_001680"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE0138546.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1953..3382
                     /locus_tag="KLH32_001681"
                     /pseudo
     CDS             1953..3382
                     /locus_tag="KLH32_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3452..4228
                     /gene="yaaA"
                     /locus_tag="KLH32_001682"
     CDS             3452..4228
                     /gene="yaaA"
                     /locus_tag="KLH32_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBE0138547.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4381..4677)
                     /locus_tag="KLH32_001683"
     CDS             complement(4381..4677)
                     /locus_tag="KLH32_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE0138548.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KLH32_001684"
     CDS             complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KLH32_001684"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBE0138549.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KLH32_001685"
     CDS             complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KLH32_001685"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBE0138550.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KLH32_001686"
     CDS             complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KLH32_001686"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBE0138551.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9600..9719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KLH32_001687"
     CDS             complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KLH32_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBE0138552.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9934..10620)
                     /locus_tag="KLH32_001688"
     CDS             complement(9934..10620)
                     /locus_tag="KLH32_001688"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE0138553.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11020..11160)
                     /locus_tag="KLH32_001689"
     CDS             complement(11020..11160)
                     /locus_tag="KLH32_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138554.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11256..11972
                     /gene="arcA"
                     /locus_tag="KLH32_001690"
     CDS             11256..11972
                     /gene="arcA"
                     /locus_tag="KLH32_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBE0138555.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KLH32_001691"
     CDS             complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KLH32_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBE0138556.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KLH32_001692"
     CDS             complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KLH32_001692"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBE0138557.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KLH32_001693"
     CDS             complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KLH32_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBE0138558.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KLH32_001694"
     CDS             complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KLH32_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBE0138559.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16251..17120
                     /gene="robA"
                     /locus_tag="KLH32_001695"
     CDS             16251..17120
                     /gene="robA"
                     /locus_tag="KLH32_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBE0138560.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KLH32_001696"
     CDS             complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KLH32_001696"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBE0138561.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17816..18336
                     /gene="yjjX"
                     /locus_tag="KLH32_001697"
                     /pseudo
     CDS             17816..18336
                     /gene="yjjX"
                     /locus_tag="KLH32_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KLH32_001698"
     CDS             complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KLH32_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBE0138562.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KLH32_001699"
     CDS             complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KLH32_001699"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBE0138563.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20985..22652
                     /gene="ettA"
                     /locus_tag="KLH32_001700"
     CDS             20985..22652
                     /gene="ettA"
                     /locus_tag="KLH32_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBE0138564.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22759..22926)
                     /locus_tag="KLH32_001701"
     CDS             complement(22759..22926)
                     /locus_tag="KLH32_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138565.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(23009..23695)
                     /gene="nadR"
                     /locus_tag="KLH32_001702"
                     /pseudo
     CDS             complement(23009..23695)
                     /gene="nadR"
                     /locus_tag="KLH32_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
     gene            complement(23716..25098)
                     /gene="radA"
                     /locus_tag="KLH32_001703"
     CDS             complement(23716..25098)
                     /gene="radA"
                     /locus_tag="KLH32_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBE0138566.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25147..26115)
                     /gene="serB"
                     /locus_tag="KLH32_001704"
     CDS             complement(25147..26115)
                     /gene="serB"
                     /locus_tag="KLH32_001704"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBE0138567.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26221..26865
                     /locus_tag="KLH32_001705"
     CDS             26221..26865
                     /locus_tag="KLH32_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBE0138568.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            26893..27908
                     /gene="lplA"
                     /locus_tag="KLH32_001706"
                     /pseudo
     CDS             26893..27908
                     /gene="lplA"
                     /locus_tag="KLH32_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            27940..28203
                     /locus_tag="KLH32_001707"
     CDS             27940..28203
                     /locus_tag="KLH32_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0138569.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28364..29083)
                     /gene="deoD"
                     /locus_tag="KLH32_001708"
     CDS             complement(28364..29083)
                     /gene="deoD"
                     /locus_tag="KLH32_001708"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBE0138570.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29140..30363)
                     /gene="deoB"
                     /locus_tag="KLH32_001709"
     CDS             complement(29140..30363)
                     /gene="deoB"
                     /locus_tag="KLH32_001709"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE0138571.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30415..31737)
                     /gene="deoA"
                     /locus_tag="KLH32_001710"
     CDS             complement(30415..31737)
                     /gene="deoA"
                     /locus_tag="KLH32_001710"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBE0138572.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(31864..32643)
                     /gene="deoC"
                     /locus_tag="KLH32_001711"
     CDS             complement(31864..32643)
                     /gene="deoC"
                     /locus_tag="KLH32_001711"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBE0138573.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            32901..34442
                     /locus_tag="KLH32_001712"
     CDS             32901..34442
                     /locus_tag="KLH32_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBE0138574.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34414..35277
                     /locus_tag="KLH32_001713"
     CDS             34414..35277
                     /locus_tag="KLH32_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBE0138575.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35491..36273)
                     /locus_tag="KLH32_001714"
     CDS             complement(35491..36273)
                     /locus_tag="KLH32_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0138576.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36270..37343)
                     /locus_tag="KLH32_001715"
     CDS             complement(36270..37343)
                     /locus_tag="KLH32_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBE0138577.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37465..37626)
                     /locus_tag="KLH32_001716"
     CDS             complement(37465..37626)
                     /locus_tag="KLH32_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBE0138578.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(37743..38348)
                     /gene="osmY"
                     /locus_tag="KLH32_001717"
     CDS             complement(37743..38348)
                     /gene="osmY"
                     /locus_tag="KLH32_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBE0138579.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(38741..40330)
                     /gene="prfC"
                     /locus_tag="KLH32_001718"
     CDS             complement(38741..40330)
                     /gene="prfC"
                     /locus_tag="KLH32_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBE0138580.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40421..41098)
                     /gene="yjjG"
                     /locus_tag="KLH32_001719"
     CDS             complement(40421..41098)
                     /gene="yjjG"
                     /locus_tag="KLH32_001719"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBE0138581.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41113..41559)
                     /gene="rimI"
                     /locus_tag="KLH32_001720"
     CDS             complement(41113..41559)
                     /gene="rimI"
                     /locus_tag="KLH32_001720"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBE0138582.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(41528..41941)
                     /gene="holD"
                     /locus_tag="KLH32_001721"
     CDS             complement(41528..41941)
                     /gene="holD"
                     /locus_tag="KLH32_001721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBE0138583.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42044..43075
                     /gene="rsmC"
                     /locus_tag="KLH32_001722"
     CDS             42044..43075
                     /gene="rsmC"
                     /locus_tag="KLH32_001722"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBE0138584.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43352..43438
                     /locus_tag="KLH32_001723"
     tRNA            43352..43438
                     /locus_tag="KLH32_001723"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43386..43388,aa:Leu,seq:cag)
     gene            43467..43553
                     /locus_tag="KLH32_001724"
     tRNA            43467..43553
                     /locus_tag="KLH32_001724"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43501..43503,aa:Leu,seq:cag)
     gene            43588..43674
                     /locus_tag="KLH32_001725"
     tRNA            43588..43674
                     /locus_tag="KLH32_001725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43622..43624,aa:Leu,seq:cag)
     gene            complement(43713..43949)
                     /locus_tag="KLH32_001726"
     CDS             complement(43713..43949)
                     /locus_tag="KLH32_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBE0138585.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44090..44878
                     /gene="fhuF"
                     /locus_tag="KLH32_001727"
     CDS             44090..44878
                     /gene="fhuF"
                     /locus_tag="KLH32_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBE0138586.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(44916..45593)
                     /gene="bglJ"
                     /locus_tag="KLH32_001728"
     CDS             complement(44916..45593)
                     /gene="bglJ"
                     /locus_tag="KLH32_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBE0138587.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(45551..>46118)
                     /locus_tag="KLH32_001729"
     CDS             complement(45551..>46118)
                     /locus_tag="KLH32_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0138588.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        10923 a        12560 c        11617 g        11018 t
ORIGIN      
        1 ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg gtctgaatgc
       61 agcaaccact tctggtgccg tttcaacgta tggcccttcc agcaactgaa tgcagtacgg
      121 tacgctggca aaaataccat gcaccacaac gttaccctca gcgtccttca caccttccag
      181 cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct gtttgcgaat
      241 cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga tctggcgcat
      301 ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac
      361 gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac tcatttcatc
      421 caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta agcgggtttc
      481 cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgctg gaatgccttt
      541 atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa tacgtaaagt
      601 attcatcaag atttttccgt ttaaacaatc tcgttaaaca gaatgataca ctgcgaaggg
      661 agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg ctaagaatga
      721 ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa acttacggat
      781 accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca ggaactcgga
      841 ctcagtgata cgcgccggac gcgctttcac ttcgccggtg taagacagtt tacgttcgat
      901 agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca gacggtcaca
      961 gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa ccacggtttc
     1021 ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc ccggatcttc
     1081 tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa tacggccaac
     1141 aaacggcgag atcaggaaca cgcccgcctc cgcacaagca cgagcctgag cgaaggagaa
     1201 cagcagggtc aggttacagt tgataccttc tttttccagc tgttctgcag cacggatacc
     1261 ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg catcgttgta
     1321 gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg aaagacgcgc
     1381 atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac caatatttac
     1441 tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc
     1501 ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg cgttaagaat
     1561 gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg cgatgtcccc
     1621 agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg tcatgatagt
     1681 atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg atatcacgac
     1741 gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt ttcggatttc
     1801 tgacactgcg ttgatatcgc ccaccatttt tatacaaaac ctcatgtatg ctacgcagaa
     1861 gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt tacacaggaa
     1921 agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc tttattaaca
     1981 gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt tggttcactt
     2041 ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt cttaaaaata
     2101 gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc agtcttgcgg
     2161 cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc ggtggaccag
     2221 gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat ggcgacctcg tttgccgaat
     2281 gttcccttgc acaactttat aaagaacgtg acgtcaatgg gcagtttcgt ggcggaccgg
     2341 catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc gccgtctttt
     2401 tgctcatcgc ctacggcata attttcagcg gagttcaggc gaacgccgtt gcccgagccc
     2461 tgagtttttc ttttgatttt cctccgctgg tgacgggcat tattctcgct gtctttgctc
     2521 tgctggcaat cactcgcggt cttcatggcg tcgcccggct catgcagggg tttgtcccgt
     2581 tgatggcgat aatctgggta ctgaccagcc tggtgatttg cgtaataaat atcgggcaac
     2641 ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa gcggcaggcg
     2701 gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc agtatgtttt
     2761 ccaatgaggt gggaatggga tcgacgccaa acgcggcagc ggcagctgca tcctggcctc
     2821 cgcatccggc agcacaaggg attgtccaga tgattggcat ttttatcgac accctggtca
     2881 tctgtacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc tacatgccgc
     2941 tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc tagggtgctg
     3001 aatttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc aactacattt
     3061 atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc tggtgtttgc
     3121 ggatctgcac cttcgcaacg atcatcggcg gcaccttgct aagccttccg ctgatgtggc
     3181 aactggcaga tatataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct
     3241 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
     3301 cccggtgttt gatccgttgc gttatccgga tatcggtcgc caactttctc cggacgcgtg
     3361 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
     3421 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
     3481 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
     3541 ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat
     3601 gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
     3661 tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg
     3721 cttgcaggcc gagaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
     3781 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
     3841 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
     3901 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct
     3961 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
     4021 gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa
     4081 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
     4141 gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact
     4201 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg
     4261 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga
     4321 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat
     4381 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg
     4441 gtggcgcggc ggtgacggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg
     4501 ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc
     4561 acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc
     4621 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
     4681 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga
     4741 cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat aatttctcct
     4801 ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa
     4861 acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa
     4921 cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag
     4981 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
     5041 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
     5101 acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat
     5161 tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat
     5221 tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc
     5281 gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg
     5341 cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt
     5401 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg
     5461 gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag
     5521 caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc
     5581 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
     5641 agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc
     5701 tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc
     5761 agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg
     5821 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
     5881 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
     5941 cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga
     6001 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
     6061 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
     6121 tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta
     6181 gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg
     6241 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
     6301 acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcactaccg cgccgatttc
     6361 cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc
     6421 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
     6481 gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
     6541 aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca
     6601 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
     6661 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc
     6721 cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt
     6781 catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
     6841 catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg
     6901 ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg
     6961 tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa
     7021 tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc
     7081 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
     7141 gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca
     7201 gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt
     7261 tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat
     7321 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc
     7381 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct
     7441 cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
     7501 gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat
     7561 ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac
     7621 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
     7681 acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc
     7741 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca
     7801 actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgta acaacgtgga
     7861 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa
     7921 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
     7981 acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac
     8041 tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct
     8101 tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
     8161 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
     8221 cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct
     8281 gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
     8341 tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
     8401 ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
     8461 gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg
     8521 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg
     8581 ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca
     8641 gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac
     8701 ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg
     8761 gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
     8821 ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga
     8881 ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg
     8941 ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg
     9001 cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc
     9061 gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt
     9121 cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg
     9181 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc
     9241 ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag
     9301 ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg
     9361 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
     9421 caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct
     9481 gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg
     9541 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
     9601 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
     9661 cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat
     9721 ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt
     9781 atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag
     9841 ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa
     9901 tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc
     9961 atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg
    10021 taaccagtcg accagtttta tgtcatctgc cactgccaga gccgtcagca atgccatgac
    10081 tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg
    10141 ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga
    10201 aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt
    10261 agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt
    10321 ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact
    10381 acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa
    10441 aactttaata ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc
    10501 caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg
    10561 cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat
    10621 attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc
    10681 ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa
    10741 aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc
    10801 ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt
    10861 tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt
    10921 agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg
    10981 aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt
    11041 taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct
    11101 aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat
    11161 gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc
    11221 ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg
    11281 ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg
    11341 atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca
    11401 acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac
    11461 tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata
    11521 aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg
    11581 aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg
    11641 aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc
    11701 gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca
    11761 tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa
    11821 tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc
    11881 gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag
    11941 gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc
    12001 gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt
    12061 tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt ctatattacg
    12121 agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc
    12181 gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgaggat
    12241 aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa
    12301 cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc
    12361 cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg
    12421 taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag
    12481 cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac
    12541 tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc
    12601 accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc
    12661 ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa
    12721 ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt
    12781 cagtgccata ttcagctgca ggttctgctt acgccagtcc ccttcgggta aagggatatg
    12841 cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt
    12901 aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa
    12961 gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc
    13021 ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc
    13081 ttccacgttc tgattgccat caaccatcaa tgactcaggt aacaaaaaat ggataaaact
    13141 tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat
    13201 agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc
    13261 cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat
    13321 caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa
    13381 catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag
    13441 ctatgtgaag tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg
    13501 cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc
    13561 gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag
    13621 cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc
    13681 cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag
    13741 taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg
    13801 cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg
    13861 gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct
    13921 tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt
    13981 gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc
    14041 acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat
    14101 atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag
    14161 ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata
    14221 gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag
    14281 tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac
    14341 cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc
    14401 cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt
    14461 gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc
    14521 ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata
    14581 gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg
    14641 ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc
    14701 gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct
    14761 tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc
    14821 caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa gcccctcagg
    14881 ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc
    14941 ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct
    15001 tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac
    15061 gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc
    15121 tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag
    15181 aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac
    15241 ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca
    15301 cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac
    15361 aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc
    15421 tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc
    15481 tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc
    15541 gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta
    15601 atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg
    15661 ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt
    15721 tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc
    15781 ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc
    15841 cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga
    15901 tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg
    15961 accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg
    16021 atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg
    16081 tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc
    16141 ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta
    16201 tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg
    16261 ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc
    16321 tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag
    16381 atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg
    16441 tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact
    16501 ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc
    16561 gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca
    16621 caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga
    16681 gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct
    16741 ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa
    16801 cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc
    16861 aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat
    16921 atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat
    16981 acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat
    17041 actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc
    17101 tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga
    17161 tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca
    17221 cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tacccatgct ggtaatccga
    17281 gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc
    17341 cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca
    17401 ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt
    17461 tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt
    17521 cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg
    17581 tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac
    17641 gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc
    17701 cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta
    17761 acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca
    17821 ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca
    17881 cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc
    17941 ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc
    18001 ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga
    18061 cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc
    18121 aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc
    18181 cgtgagtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
    18241 tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg
    18301 agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca
    18361 tccgacattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    18421 tcaatcgctt ttcagcaaca cctcttccag ccactgacgc agctccacgg gcgcggcttt
    18481 caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
    18541 acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
    18601 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
    18661 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
    18721 tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa
    18781 tgactccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag
    18841 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg
    18901 tagtaagcat cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata
    18961 ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc
    19021 cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
    19081 taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc cagcaattgc
    19141 ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc
    19201 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc
    19261 tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt
    19321 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg
    19381 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
    19441 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggta
    19501 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
    19561 gtgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc accgatgcgt
    19621 tgtgctgcaa ccatcgggta gaaaccacgc tgttgtatga gttgatgcaa aatctctttc
    19681 gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg ccattcatct
    19741 ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
    19801 gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg
    19861 gcgtcatcgc gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa acgccaggcg
    19921 acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg ggcaagcgac
    19981 gggatcatca gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa cgccaccgcc
    20041 gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag taccgtatta
    20101 gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc
    20161 cctgccagca ctgttaccag gcctgtgtta cccgctttca tcgccagacg gatacgctct
    20221 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
    20281 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
    20341 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
    20401 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
    20461 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
    20521 accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc caggtaggga
    20581 taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat ctgtcgatta
    20641 tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
    20701 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt
    20761 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
    20821 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
    20881 ttttactgcg aaggtgatcg accaactggg attaggcggg gaaacaggct acacttcgcc
    20941 tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt
    21001 ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct
    21061 ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt
    21121 ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc
    21181 agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg gaacacaccg
    21241 tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa cgcctggatg
    21301 aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag
    21361 gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc
    21421 gtgcggcgga tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac ctctccggtg
    21481 gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc
    21541 tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa cgcttcctgc
    21601 acgacttcga aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc gataacgttg
    21661 caggctggat cctcgaactg gaccgcggtg aaggtattcc gtgggaaggt aactactcct
    21721 cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc
    21781 gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga
    21841 aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac cagaaacgta
    21901 acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag
    21961 tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg agcttctcga
    22021 tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt
    22081 tccgtatgat ctccggtcag gaacagccgg acagcggcac catcactttg ggtgaaacgg
    22141 tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg
    22201 aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg
    22261 cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt ggcgaacttt
    22321 ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc
    22381 tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg
    22441 ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc
    22501 gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta
    22561 actttactga gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg ctggagccga
    22621 agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgcgaac
    22681 gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta ggcctgataa
    22741 gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc gtgaacacct
    22801 tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc gtgcaggttt
    22861 tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa
    22921 actcatttta tacctcgcct ttgagcagtt tcgtcacggt tatccttgct cccccatcat
    22981 ctcccgcacc agctccacgc agcgcaggaa acggcttcac ttcggtagga atatattccc
    23041 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    23101 gatcgaccag caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt
    23161 ccgaggtgta gatcaggtcc ggctggatcc ccttttcagc cataaacttt ttgatgccgt
    23221 tgctccacac atcccagcca tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    23281 tacggatatt tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg
    23341 tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac
    23401 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    23461 cggtatgcag cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgccggggaa
    23521 attcaagccc caaaaaacgg tgcaatgcct ccagcttttg cgcgctgggg cttttaattt
    23581 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    23641 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    23701 cctccgtatc tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    23761 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    23821 ccggcgaaaa ccgtgtttcg ccgcttcaga aatccgctcc tggccactgg gcaccgggcg
    23881 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc
    23941 acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg ccgctggttt cggttacctt
    24001 cacgccgccg accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg
    24061 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    24121 cggattcgcc attatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    24181 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    24241 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    24301 ctcattcacc gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc
    24361 atcgccatcc agaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag
    24421 cgaaccatct ttggttacgt gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa
    24481 acgcgtcaga taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac
    24541 atccgccata tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc
    24601 ggcaatcagg cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    24661 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata
    24721 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc
    24781 cgcaccaggg ttaccgccaa tcagaatggc acttcctggc accacgccgc ctcctagtac
    24841 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    24901 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    24961 cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    25021 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    25081 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc
    25141 aggcaattac ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc
    25201 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    25261 cagccctgct gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg
    25321 cgccagcggg atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc
    25381 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    25441 ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc
    25501 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gctgcgttaa
    25561 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    25621 cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    25681 ttcggttacc tccgccacca tctcgcccgt tccggccagc ttggcaattt catcaataca
    25741 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    25801 cgggattttc cccagcgggg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg
    25861 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    25921 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    25981 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    26041 accacttaat gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca
    26101 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    26161 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    26221 atggctcgca caaaactgaa attccggcta catcgggcag tgattgtcct gttctgtctt
    26281 gccttgttag tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag
    26341 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt
    26401 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    26461 ttaacagatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    26521 gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca
    26581 ggcggctatt ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc
    26641 tatctgcgcc tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    26701 accactaaca ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc
    26761 cgcacgctgc tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc
    26821 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga
    26881 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    26941 aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt
    27001 ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt
    27061 aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtgg cggtggcggc
    27121 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    27181 gataaaactc tctccacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc
    27241 gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca
    27301 ggctcggcct atcgcgaaac caaagatcgc ggcttccacc acggcacctt gctactcaat
    27361 gccgacctca gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    27421 ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc
    27481 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcacgtgg
    27541 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    27601 cccgtcagag tagctgagag tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    27661 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    27721 cccgcgccca ggtatttacc gacagcctca acctcgcgcc gctggaagcc ctcgccgggc
    27781 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    27841 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    27901 taaggtaact cgctgattaa aatatccaag gaaggaaata tgatcgatct ggaaaaccag
    27961 gaacgggaga ttatcaattt aatgttcagc cagcgtattt cctggctagc cgccgtgcgt
    28021 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    28081 ctaaaacaga tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatggcagaa
    28141 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    28201 tgaatagatt cgtgaataga tacttgaatg ttttttattt tctatccata tgaaaaataa
    28261 tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc
    28321 ccactccggc ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca
    28381 gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    28441 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg
    28501 cgccaaactc tgcagccacg ccgtagatac cagccgcttc catttccacg ccgaggatgc
    28561 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    28621 acaggttacc cacgcgagca tcaacgccca gtgctttagc tgcatctact gcgttacgca
    28681 ccatgtcaaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt
    28741 tggaatcggt gcaggccccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa
    28801 ctgcgccaca ggaaccaacg cggataattt tcttcacgcc gaaatcggtg atcagttctt
    28861 tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc
    28921 ctttgtaagt accagtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    28981 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    29041 cagcgaaatc gcccatttct gcattaatgt gtggggtagc cattgtttta tcctttttgt
    29101 gacataacaa aggcggagtc aacgctccgc ccaaatccat cagaacatgg ctttgccata
    29161 ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc
    29221 acggtgaccc agtgagcccg gttttacttt cgggccatat accagtaccg gaatgtgttc
    29281 acgcgtgtgg tcagtaccgg tccaggtcgg atcgcaaccg tggtcagcgg tgaggatcag
    29341 gatgtcgtca tcgcgcagca gagacatcag ctccggcaga cggcggtcga acagttccag
    29401 acccgcagca taaccggcga cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa
    29461 gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa
    29521 cagcgcgtcc aagccagtcg ctttcacttt tttggtgata ccgcagttgg cgtagatgtc
    29581 tgcaatttta ccgacagaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac
    29641 ggtcggtgct ggcggctcaa cagccaagtc gtgacggtta ccggtacgct ggaagttacc
    29701 agctttgtca ccgataaacg gacgagcgat aacacgaccg atattgtagc cgccgttggt
    29761 cagctcttca cgggcgattt cgcacagttc gtagagttta tccagaccga aagtttcttc
    29821 atggcaggca atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt
    29881 catgtgctct tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag
    29941 gtaacccggc agattagcgc gttcgaccag tttatccagc agctcttgcg ggaagctgtt
    30001 ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg acaccggcga tttcccagtg
    30061 accagacggg gtatctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac
    30121 ttcagcgttg ccgtccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc
    30181 cagccccaga cgggtcagat ttggcagatt gagcggccct ttacgaccat tatcagcttc
    30241 gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg
    30301 ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt
    30361 catatgttct ccgtactcag tgcttcaaat ttaaatgatc agatcagtat accgttattc
    30421 gctgatacgg cgatagacag ttggtgtgct ttccggtgcg ttatcggcaa gtttaattgc
    30481 cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcga ggataaccgc
    30541 cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct
    30601 gtaatcgatg gtgtcagatg cctgacgacg accgccgccc attgcaacca ctgccatccc
    30661 cagcgcgcgg gtatccattt cactgacaaa accttcggta tcagcataga ctgctttcgt
    30721 cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg
    30781 tgccgctacc atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa
    30841 tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg gagatcagca tctccacgca
    30901 cagcgccatc gtgacatcaa acagacgcgg gttacgatac tcacccgtca ggaactgcac
    30961 cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat tcatgtcggt
    31021 gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag
    31081 ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cccactttca cgtccatcac
    31141 cagcgcgtcc agaccttccg ccagtttctt cgccaggatc gaggcagtga tcagcgggat
    31201 ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa cgtttatcag ccggagccag
    31261 tgaactggtc tgaccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt
    31321 gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt
    31381 atgaccgagg ccgcgaccgg agatcatcgg aatatagccg ccgcaggctg cgaccatcgg
    31441 ccccaacatc agcgaagtga catcgccgac gccgccggtc gagtgtttat caacaatcgg
    31501 gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca tcgccatggt
    31561 cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc
    31621 aatctgccct tcggaaatag tgttgtcgcg aataccgttg ataaagaaac gaatttcttc
    31681 atcgctcagc gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt
    31741 aacctcccga ggaaaagagt atgaggcggc ttttgccgga tgcgtctacg ccttatccgg
    31801 cctacgggta gatacgtctc gtaggcctga taagcgcagc gcatcaggcg taaagcatct
    31861 tacttagtag ctgctggcgc tcttaccgtc gccgtggccc agcgctttca gcaggcttgc
    31921 cagcaggctg gaagcaccaa agcggtagtg acgcgcatca gcccagtcag caccgaacag
    31981 ttcatctgcg atggcgagat atttctgcgc atcttccgca gtacgcacgc cgcccgccgg
    32041 tttgaaacca acggtttttt ctacgcccat atcacggatc acttccatca tgatgcgagc
    32101 gctttccggc gtcgcgttca cagccacttt accggtagag gttttgatga agtccgcacc
    32161 cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca gttcgccggt
    32221 ttcgatgatc actttcagca gtacattcgc tgccgcgcaa gcctctttac aggctttcac
    32281 caggtcaaaa ccaacctgct cgtcacccgc catcagcgcg cggtacggga acaccacgtc
    32341 aacttcatca gcaccgtagg cgattgccgc acgggtttct gccagcgcga tttcgatgtc
    32401 gtcgttaccg tgtgggaagt tggttaccgt agcgatacgg atttccgggg tgccctgctc
    32461 tttcagagtt ttgcgagcaa tcgggataaa acgaggatag atacagatag cggcggtatt
    32521 gccgaccgga gttttggcct gatgacacag agcaattact ttctcgtcgg tgtcgtcgtc
    32581 attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt
    32641 catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt tagtattcta
    32701 acatctactc cgcaacacac ttcgatacac atcacaatta aggaaatcta cttacaagtt
    32761 tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg cgtcagtggt
    32821 ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc ttacaatggc gcatcacctt
    32881 cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc
    32941 agcaaattgt caccagccca gtgcttagcc cggagcagaa gcgccatttt ctggcactgg
    33001 aagcagaaaa caatctgcct tacccacagc ttcctgccga agcccgccgc gcgctggatg
    33061 aaggtgtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac
    33121 ccgattacgc tcgttttctg gcgaacggtt ccgaatggct ggagctggaa ggcgcgaaag
    33181 atcttgatga cgcactctct ctgctgacca ttctttacca ccacgtaccg tcggtcacat
    33241 cgatgccggt ctacctgggg caactggatg cgttgttgca accgtatgtt agaattctaa
    33301 cacaaaacga gatcgatgtt cgaataaaac gtttctggcg ttacctcgac agaaccctgc
    33361 cagatgcctt tatgcacgcc aatatcggcc cgtctgattc gcccattacc cgcgcgatct
    33421 tacgtgcaga cgcgcaggtt tcaccgaacc tgacctttat ctacgatcct gaaatcaccc
    33481 ctgatgacct gctgctggaa gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg
    33541 ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt
    33601 acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac ctgaaagcca
    33661 ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg cactactgcc
    33721 agcagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct
    33781 ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt
    33841 ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgagaaagaa gggattgccg
    33901 cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta tcgcatcagc gcgcaactgg
    33961 cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc atgttacacg
    34021 cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg
    34081 atgagccaga tccgatcacc catctgcaaa ctgtcgcccc gcatcatgct tattattatt
    34141 ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaacccg caggcactgg
    34201 tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg
    34261 gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggattta gaaaaatatc
    34321 gcgccgaagg ttcacgcacc aacaccacct ggctgggcga agaagccgca cgcaacactc
    34381 gtattctgga acgccagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt
    34441 aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag
    34501 ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg ttgcaatgac
    34561 tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc agattgttga cggcaaagtg
    34621 gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc tgaagatgtg tccgcaacat
    34681 gccacaccaa tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt gcgcaaagcg
    34741 gtgctgttta ttgaagggat aacggtaagc ggcggcgaag ccacgaccca actgccgttt
    34801 gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac tgcgccatct cacctgtctg
    34861 gtggacagta acggtatgtt gagcgaaacc ggctgggaaa aattactccc ggtgtgtgac
    34921 ggcgcaatgc tcgatctcaa agcatggggt agcgaatgtc atcaacaatt aaccggacgc
    34981 gataatcaac agattaagcg cagcatctat ttgctggcag agcgcggcaa gctggcggaa
    35041 ctgcgtttgc tgatgatccc aggccaggtg gattatttgc aacacatcga agaactggcg
    35101 gcgtttatca agggacttgg tgatgttccg gttcgcctga acgcgtttca tgcccacggc
    35161 gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg aagacgttga gccgttggct
    35221 gatgcattaa aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta tttgtgacat
    35281 gccggatacg gcctacggtt cggcacagac ttgtaggcct gataagacgc gtcaagcgtc
    35341 gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa cgccttatcc ggcctacggt
    35401 tcggcacaaa cctgtaggca tgataagacg cgttaagcgt cgcatcaggc attatgcgcc
    35461 gactgccgga tgctgcctac gaagggcaaa ctacccacga acgttaaaca ccgcatacgt
    35521 gttattaagc aacacttcgg caatctcatc cgcgggttcc gggcgtaact cgcaaagcac
    35581 ggcaaacaca cgggcagcct gttccgggcg gttaggctgc ccctgaaaac cgttgagcgg
    35641 catatccggc gcatcggttt ccagcaacaa cgatgccagc ggtaattttg cgatgacatc
    35701 gcgggtttta ctggcgcgtg gataggtgat agtaccgcct acgccgattt tgtagcccag
    35761 ctgtacaaac cgttcagcct gttgctggct gccagaaaat ccgtggacaa caccagcacg
    35821 cggcaaatcg tggtgtttaa gatgcatcgc cagtttgtcg tgcgtgcgcc gtgaatgcag
    35881 gatcaccggc agatcgtagc gtttcgccag tttcagttgt tcgtcgagta accactgctg
    35941 cctctcaaat tgcggatcgt cgccgaagag atccagaccg atctccccca ccgccaccac
    36001 cttcgccgga cgcctttcca gcgcctgctg tagctgctca agagacacat cgctatgttt
    36061 ttccaacata ccaggatgca agcccaatgc ggcatacagc ggttgataat tttccgctaa
    36121 cgcctgcacg cgggcaaaat tttccgcctc tgtcgccgga acaatgatct tgcctacgcc
    36181 cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca ataaaaggcg ggaaatcaaa
    36241 atggcagtga gtgtcgataa aacggcaaat caagccagat cctcattgtt aaatgtcggg
    36301 tcgttcgcct gcggagcgtc gctcacttcg gcaaccagcg tatcgttggc gactggcgca
    36361 ggcggaatga caatcgattc cggcgtcacc actggcacca gatggcgggt aagcggcgct
    36421 ttttcagtta atagcttgcc aaccgtggcg aggaaataac gaccgcataa acgcccaagt
    36481 ttatagtctt cacgcagcgc cggaatccga ctgccaagcg cgatactatg taatggcttc
    36541 ggcggataaa tttcggatat ccgcagcttg cccggtggtt tctcaataaa ttgctgaatg
    36601 tcacgatagc tggtttcatg atgctgcacc agattgacca gcggctgcag gctactgtca
    36661 cccagccagc gttccatgcg tttgaaccac tgcggcgtgt agtacatttg tgacggcaca
    36721 gtgcggatga cgaccaacgt tttagcgccc tgccttgccg cctctttaac cggaatcgca
    36781 tcactgatcc cgccatccag gtagttaatg ccttccagcg acactccgct acgataaaag
    36841 ccaggtatcg cactggaggc gcgaatcaca tccagccagt tttgtttggt tggtaaaaag
    36901 taattcggct cgtagtcatc ctgacgacag gcgcacatat aaaacgattt gccgctgtca
    36961 aacaaccgcg cggcggtatc catttgcaac ggcatctggc ttgcggtggc ctccaccagc
    37021 cagtcgagat cgataagatt tcctccacgg acaaagcgca atggatcgaa aaattcgcgt
    37081 tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg gctgattgca gataaacgcc
    37141 gagaggttct gcgccccggc agatgtgccg agataaagat cgaaaggatt aaactgcgcg
    37201 cgcataaact catccagcac gccagccgtg aaaattccac gctgtccgcc gccttcgcac
    37261 accagagcta ttcgtccagg ctggaacggc cttagcgaca acggcgcaat attaccaagc
    37321 gttacaggta ttcgctgccc cacttctgcc ttcctgtttt tgttgtgaaa gaaagacaaa
    37381 gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt ccgctggact ggctttggat
    37441 aaaattttct taatggtctg aaatctaggg tcgttttcgg cccatgaaca aactcactag
    37501 gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg taccggccag
    37561 accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga tgccccaacg
    37621 aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca
    37681 ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggacg
    37741 aattacttag ttttcagatc atttttaacg cttttcacac catctaccgc tttggcgata
    37801 ctttcagcac ggtcactttg tgcctgagaa tctacggtac cggagagctg aaccacgcca
    37861 tcggtggttt caactttcac tttacgtgaa gggacgatat cgtccgccag cagtttggct
    37921 ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct tcaccgagcc ttctttagcg
    37981 tcgcgaacgt gcagtttgtc gctgacagag gtcacgcctt caacgccttt cgccactttc
    38041 actgcctctt cggcctgggc ctgactttca acgaaaccgc tcagggtcac gactttttga
    38101 tcggttttga cagagatatc ggtgctcttg atgttgtcat gatccaccag cgccgccttc
    38161 actttcgcgg tgatggcgct gtcatccatg aaattaccga ctttattcat agagctatcg
    38221 actttttgcc ctgcgctttc attggtagtc tgcgcgttgt tttccgcgta ggcagagccg
    38281 gtcgcgacgg cagaggtcaa cattacagcc agcagagttt tcgaaatctt cagtcttgtc
    38341 atagtcatcg atttattcct gtatgtttgc tcgtaatttg agcctagcaa cacgaggttg
    38401 cattgctgaa tagggagaga cttcaccctc tacagaagtc aatggtcgcc atcacaaaag
    38461 cgatgggtga tgaataacga ccattacagc ctctgaatca gttattaata tcggtagaat
    38521 gacaatcgac ggctttagat actgatatct acgcattgaa cggtatttaa cgccatcaga
    38581 aatgtcatca ctttgttaaa tatagatcac aattttgaaa ccgctcggga tatcagcgaa
    38641 aacataagca aaagtgaatg ttttaagaac attccgtaag cggctaataa ggaagggaaa
    38701 ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa ttaatgctcg cgggtctggt
    38761 ggaactgaac gtccggataa cgttcctgtg ccaggcgcag gttgaccatg ctggtagcga
    38821 tgtaagcgag gttatcgccg ccatcaagcg ccagttggct ttcgttctta cgcttgaact
    38881 cttcgaattt cttcgcgtct gcacattcta cccagcgggc agtggcaacg ttgactgact
    38941 catacactgc ttcaacgttg tattcgctct tcaggcgcgc taccaccaca tcaaactgca
    39001 gcacaccaac cgcaccaacg atcagatcgt tgttggagat tggacggaac acctgcaccg
    39061 cgccctcttc ggaaagctgt accagccctt tgagcagctg tttttgcttc agcggatctt
    39121 tcaggcggat acgacggaac agttctggtg cgaagttcgg aataccggtg aacttcatca
    39181 tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc gtggttgtgc aggccgagga
    39241 tatcgcccgg atacgcttct tcaacgtgcg aacggtcacc cgccataaag gtcagcgcgt
    39301 cggagatcac cacatctttc gcagtgcgca cctggcgcag tttcatgcct ttttcatatt
    39361 taccggacac cacacgcata aacgccacgc ggtcgcggtg tttcggatcc atgttggcct
    39421 gaattttaaa tacgaagccg gtaaatttat cttcgctcgc ttctacggta cgggtatcgg
    39481 tctgacgcgg catcggcgca ggcgcccact ccaccaggcc attcagcata tgatcaacgc
    39541 cgaagttacc cagcgcagta ccgaagaata ccggagtgat ttcgcccgca aggaacagct
    39601 ctttgtcgaa ctcgttagac gcgcctttaa ccagttccag ttcgtcacgc agctgctgtg
    39661 ccagatcttc accaaccgca gcatcgagat ccgggttatt cagcccttta acaatgcgga
    39721 cttcctgaat ggtgtgccct ttaccgctct gatagagata ggtttcatct ttataaaggt
    39781 ggtaaacgcc tttaaacagc ttgccgcagc caattggcca agtgatcggt gcgcagccaa
    39841 ttttcagctc gttctcaact tcatcgagca gttccatcgg gtcgcggata tcacggtcaa
    39901 gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg ggtaacttcc atcagcttac
    39961 gggtacgatc ttcaacacct tttgcggcgt cgataaccat caggcagcag tccaccgccg
    40021 tcagggtacg ataggtatct tccgagaagt cttcgtgccc cggggtgtcg agcaggttaa
    40081 ccaggcaatc gtgatacgga aactgcatca cagacgtagt aatggagatc ccacgctgct
    40141 tttccatctc catccagtcc gacttagcgt gctggttgga accacggcct tttactgtac
    40201 cggcggtctg aatggcctgt ccgaacagca gcaccttctc ggtgatggta gttttaccgg
    40261 cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc cacctctttc aaataaggag
    40321 acaacgtcat aattctttct tcttgagtaa gcggcatcgc gccgcgcttg ttgaaaacga
    40381 aaaattgcgg ctattttacc catcaacggg ggggaggcaa tcagtgttta cacaggagct
    40441 gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc gatgccttct ggttgctcgc
    40501 gattgtgcgc attcagccag caggtcgcaa gcccggcgtt gatgccaccg agaatatcgg
    40561 actcggcagt gtcgccaacc atcagcacgc gtgaacggtc aggattgccc gcctgttcca
    40621 gcgcataatc gaaaattttc ttattcggtt tggcaacgcc aacttcttcg gaaatcacca
    40681 gcaaatcgaa gtaatcacgc aggcccgtcc gttccagacg cacttgctgc aaggcgctaa
    40741 aaccgttggt gatgatgccg attttagcgt tgctacgaat ggcgttaagc agagaaaccg
    40801 cgcccggcaa cggcgtacag atttccgcca tcgcattaat aaaggcatcg ttaagcaagc
    40861 ctgcttcaac tttcagccgt tcggcccagc tctcgaaacg cccgtgctga agctgtaatg
    40921 aagtgatcgc gccgttttga taatccaccc acagtggctt gttaacggcc tgatagtcct
    40981 gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa catccgctgc aggccggtga
    41041 atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa aatccagtcc cacttcatta
    41101 ttccaccttg tcttacatac tgattggcaa cgccatgatg atggcgtctt cgcgaccgtc
    41161 cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta aagcctaaac tttcgtacag
    41221 ggcaatggcg gcagcatttg aggcacggac ttccagccat agtgtcgcca cgccgcgttt
    41281 ttccagttcg tcgatcagat gttccagcag cgccctaccc agcccctgac gctgatagtc
    41341 gggatcgacc gcaatattga acaatgtagc ttcatccagc accacttgcg taatcgcaaa
    41401 cgccgccatt ttgccgtttt gcgttaactg aaagttgaga taacgctcgc cctggttgct
    41461 ggcaaaggtt ttttcactcc acggaaagga gtgggcgcgt tgttcaatgt ggtaagccgc
    41521 tggtaaatca gtcgtttcga gggaagaaat cgtgttcata tgtgcaaatt tgttgccata
    41581 acgcggcgcg tgccgttggg tttgcccgta attcggtgag cgccggtgat gccacctgag
    41641 cgccttccaa tgatagcggt tcgtcagtac ccaaccgcca gctgttgcag cgactgcctt
    41701 gcggcagcat cgcgattttt tctggcgtca gttgcagcac ttgatcgggg ctgacggtta
    41761 atgcgcgcag aacatcgctc actaaaggat cagtcagggc gggaagatcg tttgccacca
    41821 tcaccagacg gacgtgtgcc gggctcgcaa tggcaatctc cccctgcaac gcgccagggc
    41881 gacgcagcga ccactgggta atgcccagtt gctgtaactg ccagtctcgt cgggatgtca
    41941 tagcgaatcg ctcctgttgt caggggcgca aatatagcaa attcgtcgat accgcgccaa
    42001 catatggcta taatcgccgc cagtatcaat tgaggagcat tccatgtctg catttacccc
    42061 ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa caaagccgta ttctgtttgc
    42121 cggagactta caggatgacc tgcccgcgcg tttagatacc gcggccagcc gtgctcatac
    42181 ccagcaattc caccattggc aggtgttaag ctgccagatg ggggataacg cccgttttag
    42241 tctggtcgcc acggcgaatg atgtcgcaga ttgcgatacg ctgatttact actggccgaa
    42301 gaacaaaccg gaagcccagt tccagttgat gaatttactt tctctgctgc cagtggggac
    42361 agatattttt gtcgttggcg agaaccgcag cggcgtgcgc agcgctgagc agatgctggc
    42421 agattatgcg ccgttgaata aagtcgacag cgctcgtcgc tgtggcctct attttggtcg
    42481 tctggaaaaa cagccagtat ttgatgcgga taaattctgg ggcgaataca gcgtcgatgg
    42541 cctgacggtc aaaacgctgc ctggcgtgtt tagccgcgac ggtctggatg tcggtagcca
    42601 gttgctgctc tcgacgttaa ctccgcacac gaaaggtaaa gtgctggatg tcggctgtgg
    42661 cgcgggtgta ctttcagttg cctttgcgcg ccattcgccg aaaattcgtc tcaccttgtg
    42721 cgatgtctct gccccggcgg tagaagccag ccgcgcaaca cttgcagcca acggtgttga
    42781 aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa ggttgttttg atatgatcat
    42841 ctccaacccg ccgttccacg atgggatgca aaccagcctg gatgcggcgc aaacgctgat
    42901 tcgtggcgcg gtgcgtcatc ttaatagcgg cggcgagctg cgaattgtag cgaacgcctt
    42961 cctgccttac ccggacgtgc tggatgagac atttggcttc catgaagtta tcgcgcaaac
    43021 cgggcgcttc aaggtatatc gcgccattat gacccgccag gcgaagaaag gttgagtatc
    43081 tgatatctgt cgccgcatac cgtgtcggat gcggcgtgaa cgccttatcc gacctacaaa
    43141 acctgcacgt tagcccttcg taggccagat aagacgcgcc agcgtcgcat ctggcatctg
    43201 cccccgcatg cctaccaaac ctgcacgcta gtttcctgat ggacattttt ccagcaatta
    43261 cacatcaggc gataattaac tattgacgaa aagctgaaaa ccactagaat gcgcctccgt
    43321 ggtagcaatt ctttttaaga attgatggta tgcgaaggtg gcggaattgg tagacgcgct
    43381 agcttcaggt gttagtgtcc ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa
    43441 aaccacgttg atattgctcg cactgggcga aggtggcgga attggtagac gcgctagctt
    43501 caggtgttag tgttcttacg gacgtggggg ttcaagtccc ccccctcgca ccaaacgagg
    43561 cgatatcaaa aaaagtaaga tgactgtgcg aaggtggcgg aattggtaga cgcgctagct
    43621 tcaggtgtta gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaattatc
    43681 tttacttcct ttcttgtttc ttccctgttt tgtcatccct gattcaaatt cattatcatc
    43741 agcatgatgc cagcaataag cgccaggcac gatggtagca aaatgtaatg ccgcaactgt
    43801 ttgtgataca gcatcaccgg cgtcaatact aatcccatca gaatgacaat ccgccactga
    43861 tccggcgata aatccgcata catcagccct gcaatcagca atcccggcag caacactccc
    43921 cagccactgc ccagcgtacg ttgcaacatg gtttcatctc tttcattgat aatgaaaacc
    43981 aatatcatat gataattttt atcatttgca agccagagaa atcccttgct atcgagtaaa
    44041 cctatcgcta ggattagcaa tcattatcat ttagattact atcacgatta tggcctatcg
    44101 ttccgcaccg ctctatgaag atgtcatctg gcgaacgcat ctccagccgc aggatgctgg
    44161 gcttgcacaa gccgtacggg cgatgatcgc caaacatcgt gaacatttgc tggagtttat
    44221 ccgcctggat gaacccgctc cgcttaacgc catgacgctg gcgcaatggt catcaccgaa
    44281 tgcgctaagt tctctactgg cggtctattc cgatcatatc tatcgcaacc aaccaacgat
    44341 gatccgcgag aacaaaccgc tgatctcact atgggcacaa tggtatatcg gcctgatggt
    44401 gccaccatta atgctggcgc tactgacgca ggaaaaagca ttagatgtgt cgccggaaca
    44461 tttccatgct gagtttcacg aaaccggacg cgtcgcctgt ttctgggtcg atgtgtgtga
    44521 agataaaaac gcaacaccac attcgccgca gcagcgaatg gaaacgttaa tcagccaggc
    44581 gctggttccg gttgtgcaag cactagaagc gaccggagag atcaacggca aacttatctg
    44641 gagtaatacc ggttatttga tcaactggta tctcactgag atgaagcaac tgctcggcga
    44701 ggctactgtt gaatcgctgc gccatgccct cttttttgag aaaacgctca cgaacggtga
    44761 agataatcca ctctggcgta ccgtggtact gcgcgacggc ctgctggtgc gccgcacttg
    44821 ctgccagcgt tatcgcttac cggatgtgca gcaatgtggc tattgtacgc tgaaatgaaa
    44881 gcactgccgg ggaagtaaac ccggcatcat gcggattaat agggatgcaa cacattactt
    44941 gcttcgcaat gccgcataca taacagaata tctgcggcct ccaacaaccc tgcatcagaa
    45001 ctgacgccca gtttcgacat cacattaaat ttatgcgctc ggatcgtttt gatattacgt
    45061 ttaagctgct cggcaatttg tgtcattgag tagccacgcg acataaagcg caatatttct
    45121 ctctccgtcg ggcttaacgt ccggctttgg ttaatgtacc actgattgtt cagtcggtcg
    45181 gtcgcctgac gtacaccatt taatgacaaa aagagttcct gatgaaaaat ctccagcgtt
    45241 gacgctttac ttaatacacc gtccagcggt gatggcgaca atgaaccaat cagccgagct
    45301 tcaatatcat catccacaat aactaaacgc cgggtacgcg gaaacttaat cgccagctca
    45361 gtcaggcaag ataatccctc tctgcgctca cttctcatgg cagaaaaaga aaaaatgacc
    45421 gccgcaaacg agatccgcga catggcatcc tgaaatgact cctgactggt ataggtatgg
    45481 agcttataag ggttgcccgc aaactttctg gataaactct caataccgat actactcatg
    45541 acgcattttt ctatgagtgc gacatttctc ttcttaattc ggctgtgttc cattctgcgg
    45601 catctccata aacagaaggc aagttaagtt ctcttgcacc ctgcgcgcta tttatccatg
    45661 catacatttc agcgttgctg tgaattgcca gtcgacgcat cgcactgttt ttttgggcgc
    45721 tgatagtttt attacttttc ttaagcaatg aagctatctg gttgatgccc catcctttac
    45781 ctaatagccg cagtaccttg cgctctgaaa gagtgagaat gacggagcga tagctaaaat
    45841 catgagtttc agtcattgcc ggagaggtca gcatggcgct gatacgctct gcgtgcgtat
    45901 tgccggaacg tacggtcttg accagattct caatgggttc aacatcagac aataacgtcg
    45961 ccgtagggca catgagcagt tctacagcct gggaatacca ggagcgtgac accatgaaaa
    46021 cccagtgaat ttcccgatat tgtgagatta aagaataata atgttcacaa acagaacgtg
    46081 gatcttcact ttcaccggct aaatcagcaa tgactaat
//
LOCUS       DAEEDS010000030        38775 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEEDS010000030 DAEEDS010000000
VERSION     DAEEDS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.030"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KLH32_001730"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KLH32_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBE0138589.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="KLH32_001731"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="KLH32_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBE0138590.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="KLH32_001732"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="KLH32_001732"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBE0138591.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="KLH32_001733"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="KLH32_001733"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBE0138592.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="KLH32_001734"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="KLH32_001734"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBE0138593.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="KLH32_001735"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="KLH32_001735"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBE0138594.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="KLH32_001736"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="KLH32_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBE0138595.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="KLH32_001737"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="KLH32_001737"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBE0138596.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="KLH32_001738"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="KLH32_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBE0138597.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="KLH32_001739"
     CDS             7138..7749
                     /locus_tag="KLH32_001739"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBE0138598.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KLH32_001740"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KLH32_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBE0138599.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KLH32_001741"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KLH32_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KLH32_001742"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KLH32_001742"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBE0138600.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="KLH32_001743"
     CDS             complement(11943..12302)
                     /locus_tag="KLH32_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138601.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="KLH32_001744"
     CDS             complement(12299..12805)
                     /locus_tag="KLH32_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138602.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="KLH32_001745"
     CDS             complement(12951..13514)
                     /locus_tag="KLH32_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138603.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="KLH32_001746"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="KLH32_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="KLH32_001747"
     CDS             complement(14722..15096)
                     /locus_tag="KLH32_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBE0138604.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="KLH32_001748"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="KLH32_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBE0138605.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="KLH32_001749"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="KLH32_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBE0138606.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="KLH32_001750"
     CDS             complement(17166..18056)
                     /locus_tag="KLH32_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBE0138607.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="KLH32_001751"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="KLH32_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBE0138608.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="KLH32_001752"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="KLH32_001752"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBE0138609.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="KLH32_001753"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="KLH32_001753"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE0138610.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="KLH32_001754"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="KLH32_001754"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBE0138611.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="KLH32_001755"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="KLH32_001755"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE0138612.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="KLH32_001756"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="KLH32_001756"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBE0138613.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="KLH32_001757"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="KLH32_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBE0138614.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="KLH32_001758"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="KLH32_001758"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBE0138615.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="KLH32_001759"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="KLH32_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBE0138616.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="KLH32_001760"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="KLH32_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBE0138617.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="KLH32_001761"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="KLH32_001761"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBE0138618.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="KLH32_001762"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="KLH32_001762"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBE0138619.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="KLH32_001763"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="KLH32_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBE0138620.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="KLH32_001764"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="KLH32_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBE0138621.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="KLH32_001765"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="KLH32_001765"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBE0138622.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="KLH32_001766"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="KLH32_001766"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="KLH32_001767"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="KLH32_001767"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBE0138623.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="KLH32_001768"
     CDS             complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="KLH32_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBE0138624.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38654..>38775)
                     /locus_tag="KLH32_001769"
     CDS             complement(38654..>38775)
                     /locus_tag="KLH32_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBE0138625.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10357 a         9708 c         8723 g         9987 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa tcttgcattg
    38641 gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc gtacgttggc
    38701 gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc cgttggactt
    38761 ttttggcgtc agcac
//
LOCUS       DAEEDS010000031        38531 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEEDS010000031 DAEEDS010000000
VERSION     DAEEDS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38531
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.031"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..74)
                     /locus_tag="KLH32_001770"
     CDS             complement(<1..74)
                     /locus_tag="KLH32_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBE0138626.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KLH32_001771"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KLH32_001771"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBE0138627.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="KLH32_001772"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="KLH32_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBE0138628.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KLH32_001773"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KLH32_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBE0138629.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="KLH32_001774"
     CDS             2704..2940
                     /locus_tag="KLH32_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBE0138630.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="KLH32_001775"
     CDS             2943..3800
                     /locus_tag="KLH32_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0138631.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5797
                     /locus_tag="KLH32_001776"
                     /pseudo
     CDS             3800..5797
                     /locus_tag="KLH32_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KLH32_001777"
     CDS             complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KLH32_001777"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBE0138632.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6400..7296)
                     /locus_tag="KLH32_001778"
     CDS             complement(6400..7296)
                     /locus_tag="KLH32_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBE0138633.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7345..7584)
                     /locus_tag="KLH32_001779"
     CDS             complement(7345..7584)
                     /locus_tag="KLH32_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBE0138634.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7687..8286
                     /gene="nemR"
                     /locus_tag="KLH32_001780"
     CDS             7687..8286
                     /gene="nemR"
                     /locus_tag="KLH32_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBE0138635.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8323..9420
                     /gene="nemA"
                     /locus_tag="KLH32_001781"
     CDS             8323..9420
                     /gene="nemA"
                     /locus_tag="KLH32_001781"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBE0138636.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9501..9908
                     /gene="gloA"
                     /locus_tag="KLH32_001782"
     CDS             9501..9908
                     /gene="gloA"
                     /locus_tag="KLH32_001782"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBE0138637.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10011..10658
                     /gene="rnt"
                     /locus_tag="KLH32_001783"
     CDS             10011..10658
                     /gene="rnt"
                     /locus_tag="KLH32_001783"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBE0138638.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10751..15209
                     /locus_tag="KLH32_001784"
                     /pseudo
     CDS             10751..15209
                     /locus_tag="KLH32_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15253..16461)
                     /locus_tag="KLH32_001785"
     CDS             complement(15253..16461)
                     /locus_tag="KLH32_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0138639.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16469..16969
                     /gene="tnpA"
                     /locus_tag="KLH32_001786"
     CDS             16469..16969
                     /gene="tnpA"
                     /locus_tag="KLH32_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBE0138640.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KLH32_001787"
     CDS             complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KLH32_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBE0138641.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17690..18517
                     /gene="mepH"
                     /locus_tag="KLH32_001788"
     CDS             17690..18517
                     /gene="mepH"
                     /locus_tag="KLH32_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBE0138642.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18645..19226
                     /gene="sodB"
                     /locus_tag="KLH32_001789"
     CDS             18645..19226
                     /gene="sodB"
                     /locus_tag="KLH32_001789"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBE0138643.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19372..20541)
                     /locus_tag="KLH32_001790"
     CDS             complement(19372..20541)
                     /locus_tag="KLH32_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0138644.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KLH32_001791"
     CDS             complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KLH32_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBE0138645.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21095..22120
                     /gene="purR"
                     /locus_tag="KLH32_001792"
     CDS             21095..22120
                     /gene="purR"
                     /locus_tag="KLH32_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBE0138646.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22117..23049)
                     /locus_tag="KLH32_001793"
     CDS             complement(22117..23049)
                     /locus_tag="KLH32_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0138647.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23162..24373
                     /locus_tag="KLH32_001794"
     CDS             23162..24373
                     /locus_tag="KLH32_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBE0138648.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24664..25812
                     /gene="cfa"
                     /locus_tag="KLH32_001795"
     CDS             24664..25812
                     /gene="cfa"
                     /locus_tag="KLH32_001795"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBE0138649.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KLH32_001796"
     CDS             complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KLH32_001796"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBE0138650.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26708..28081
                     /gene="mdtK"
                     /locus_tag="KLH32_001797"
     CDS             26708..28081
                     /gene="mdtK"
                     /locus_tag="KLH32_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBE0138651.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28122..29378)
                     /locus_tag="KLH32_001798"
     CDS             complement(28122..29378)
                     /locus_tag="KLH32_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBE0138652.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29686..29762
                     /locus_tag="KLH32_001799"
     tRNA            29686..29762
                     /locus_tag="KLH32_001799"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29720..29722,aa:Val,seq:gac)
     gene            29767..29843
                     /locus_tag="KLH32_001800"
     tRNA            29767..29843
                     /locus_tag="KLH32_001800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29801..29803,aa:Val,seq:gac)
     gene            29951..30256
                     /locus_tag="KLH32_001801"
     CDS             29951..30256
                     /locus_tag="KLH32_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBE0138653.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30382..31986
                     /locus_tag="KLH32_001802"
     CDS             30382..31986
                     /locus_tag="KLH32_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBE0138654.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(31998..32810)
                     /locus_tag="KLH32_001803"
     CDS             complement(31998..32810)
                     /locus_tag="KLH32_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138655.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32814..33599)
                     /locus_tag="KLH32_001804"
     CDS             complement(32814..33599)
                     /locus_tag="KLH32_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBE0138656.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33596..34264)
                     /locus_tag="KLH32_001805"
     CDS             complement(33596..34264)
                     /locus_tag="KLH32_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBE0138657.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34328..34966)
                     /locus_tag="KLH32_001806"
     CDS             complement(34328..34966)
                     /locus_tag="KLH32_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBE0138658.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34979..37080)
                     /locus_tag="KLH32_001807"
                     /pseudo
     CDS             complement(34979..37080)
                     /locus_tag="KLH32_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37101..37727)
                     /locus_tag="KLH32_001808"
     CDS             complement(37101..37727)
                     /locus_tag="KLH32_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBE0138659.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KLH32_001809"
     CDS             complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KLH32_001809"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBE0138660.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9280 a        10058 c         9432 g         9761 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttacttttgg tcaaatttgg cctgatggtc cagattagcg atctgtggca
     5101 atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat tgcagatgcc
     5161 caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta tcgctgtcac
     5221 taatcccccg gtgtatgatt ttgctgattt tcttaactat aatctggcaa aaatcgttgg
     5281 cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg ctcgtaaaag
     5341 ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc gccatccaac
     5401 actgagtgaa agcgaatttg aatcgctcac ttatcatcac gtcagtcagt tgagtaacag
     5461 ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc tgaactgttc
     5521 tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat cgcgagtacg
     5581 ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc agcaaaaatc
     5641 actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc gtcatcatca
     5701 acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc tttcgcaact
     5761 tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca ccacaggcat
     5821 agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg ccgccaacgt
     5881 ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc gcgatgacgg
     5941 catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct aaatgcccgg
     6001 taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg cctgcggatt
     6061 ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg gcatgaatat
     6121 ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc agacctttat
     6181 cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac gtgacgaggt
     6241 tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc gccagaatgg
     6301 ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata agtgtaaacc
     6361 agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt cgtaccccag
     6421 tgcagcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt ctgcttcgac
     6481 agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct gcgacggtaa
     6541 acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact cctccgccac
     6601 cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac gaccaccacc
     6661 aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga gcgtgccatc
     6721 cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa gggtaaacgg
     6781 cagacgtgat tgcaacaggg caaattgcgc aggcgtaaag ttcgatacgc caaaatgacg
     6841 cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg catccattaa
     6901 cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat taattagcga
     6961 ctgttcggcg ctcttaatga tgtgattgcg gtcagtgatg taatgaccaa tgacgttttc
     7021 ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt cacgcaggtg
     7081 aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac cataaatatc
     7141 agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac tgaccagctg
     7201 gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac gggaaaactc
     7261 cgggccttcc ggcgcaatag taatacgctg aaccataatc gcttcctctt atcagatata
     7321 agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt cgtctgatga
     7381 cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc gcagcacctc
     7441 ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac tacgaaaaca
     7501 gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc tctggacggg
     7561 aaagaactct aattgctccg ccacctcgcc ctcctcagat aagattatta ccattattga
     7621 agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc tactacactc
     7681 caacgcatga acaaacacac cgaacatgat actcgcgaac atctcttggc gacgggcgag
     7741 caactttgcc tgcaacgtgg attcaccggg atggggctaa gcgaattact aaaaaccgct
     7801 gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt tggcgttgcc
     7861 atgcttgagc gtcattacgc cgcatatcac cagcgactgg ctgagttgct gcaatccggc
     7921 gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa ccagttttgc
     7981 caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt gtgcgatctg
     8041 tcagaagata tgcgcagcgc gatggataaa ggtgctcgcg gcgtgatcgc cctgctctcg
     8101 caggctctgg aaaatggccg tgagaaccat tgtttaacct tttgtggcga accgctacaa
     8161 caggcacaag tgctttacgc actatggctt ggcgcgaatc tgcaggccaa aatttcgcgc
     8221 agtttcgagc cactggaaaa cgcgctggcc catgtaaaaa acattattgc gacgcctgcc
     8281 gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc tgaaaaactg
     8341 tattccccac tgaaagtggg cgcgatcacg gcggcaaacc gtatttttat ggcaccgctg
     8401 acgcgtctgc gcagtattga accgggtgac attcctaccc cgttgatggc ggaatactat
     8461 cgccaacgtg ccagtgccgg tttgattatt agtgaagcca cacaaatttc tgcccaggca
     8521 aaaggatatg caggtgcgcc tggcatccat agtccggagc aaattgccgc atggaaaaaa
     8581 atcaccgctg gcgttcatgc tgaaaatggt catatggcct tgcagctgtg gcacaccgga
     8641 cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc ttcagcactt
     8701 agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg cgttgaaaca
     8761 tccatgccgc gtgcgcttga actggaagag attccgggta tcgtcaatga tttccgtcag
     8821 gccattgcta acgcgcgtga agccgctttt gatctggtag agctccactc tgctcacggt
     8881 tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca gtacggcggc
     8941 agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat tgaagaatgg
     9001 ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa cacagataac
     9061 ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa acgcggcatt
     9121 gcttatctgc atatgtcaga accagattgg gcggggggtg aaccgtatac tgatgcgttc
     9181 cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc atacacagta
     9241 gaaaaagctg aaacgctgat cggcaaaggg ttaattgatg cggtggcatt tggtcgtgac
     9301 tggattgcga acccggatct ggtcgcccgc ttgcagctca aagctgagct taacccacag
     9361 cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc gacgttgtaa
     9421 tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt gaatctgtta
     9481 gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc
     9541 aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt accagcgaaa
     9601 acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc gaagaagcgg
     9661 tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact gcttatggtc
     9721 acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt caaaacgggg
     9781 gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc gcgtttgtgg
     9841 aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt cgcggtctgg
     9901 gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg catcgacaag
     9961 taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag atgtccgata
    10021 acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg
    10081 ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc gccatcaccc
    10141 tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc cacgtcgaac
    10201 catttgtcgg cgcaaatttg caaccagaag ccctcgcctt caacggcatt gacccgaacg
    10261 atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt aaagttgtac
    10321 gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac aacgccaatt
    10381 ttgatcacag ctttatgatg gccgccgcag aacgcgcctc gctgaaacgt aacccgttcc
    10441 accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga caaaccgtat
    10501 tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg cactccgcgc
    10561 tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc
    10621 tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc ctgatgacat
    10681 gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat attcacgggg
    10741 aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg tgttttcacc
    10801 ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc agtcgcaaac
    10861 ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg gctccgggaa
    10921 aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg gcgaagatac
    10981 ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga taaaagccct
    11041 gggcaccgac gttcagcgca acttgcagat cccgttgaag ggtattgccg atgaacggcg
    11101 gcggcgcggc gaaacggtaa ttattgatga agtccacgcg gtagcgggca gtaaacgtgg
    11161 tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct cagcacagcg
    11221 aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc ttggtggcga
    11281 tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac gaattgtcgt
    11341 accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcaccggcg aagacagcca
    11401 tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg atgaagtgtt
    11461 gcgccatcgc tcgaccattg tctttactaa ttcacgcggg ctggcggaaa aactgacagc
    11521 acgattaaat gagctttacg ccgcacgctt acagcgttcc ccgtctatcg ccgttgatgc
    11581 ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtacaaagta gcgacgtttt
    11641 tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca ccgaacaggc
    11701 gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtcttgaac tggggattga
    11761 tatgggcgcg gtggatctgg tgattcaggt ggcaacgccg ctttctgttg ccagtgggtt
    11821 acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc tgtttttccc
    11881 ccgtacccgg cgtgatttag tcgattccgc agtcattgta gagtgtatgt tcgcaggcag
    11941 gctggaaaac ctgacaccac cgcataatcc tctcgacgtc cttgcgcagc aaaccgttgc
    12001 cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac gccgtaccgc
    12061 accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc tttccgggcg
    12121 ctatccctct ggcgattttt ctgcttttcg ccccaaactg gtctggaaca gggagaccgg
    12181 gatattgacc gcccgacctg gcgctcaatt gttggcggtt accagcggcg gcaccattcc
    12241 ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaaaaggccg gttcgcggcg
    12301 ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgaca ttatcactct
    12361 cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg tgactcctgc
    12421 tccgggtcgt tctgcccagc tccccttctg gcgtggtgaa ggtaacggac gtccggctga
    12481 attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt tcttttccgg
    12541 gactattccc ccgtggctgg cagaagaaaa tacgaacgcc aatattcagg ggttgattga
    12601 cgagcaacgc aacgcgacgg gcatcgttcc ggggagtcgc catctggtcc tcgaacggtg
    12661 ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa gacgggtgca
    12721 tgaaccctgg gcgctggcga ttgccgggcg aatacatgcc ctatggggcg ctgacgcgtc
    12781 tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg gtaaattgcc
    12841 cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc gcgaggcggt
    12901 aggcagctcg gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg cattattaat
    12961 gccgggccgc acgccgggcc atcgcacccc gctttggcaa caacggctgc gcgccagtca
    13021 gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa ccctacgcga
    13081 atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc gcctgaacgg
    13141 tggcgaaatt caaatatccg atgtaacgac cactacgccc tcgcctttcg ccacaagttt
    13201 attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg cagagcgccg
    13261 ggcatccgta ctgtcgctgg acagcgagtt actgcgcaat ctactcggac aggtcgatcc
    13321 gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc aacgactggc
    13381 tcctggtaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg aactggggcc
    13441 aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagagg ttgcgtcgta
    13501 tctggaaaat cttcttgcag taaaacgaat cttcccagcg atgattagcg gacaggagcg
    13561 tcttgcctgt atggatgatg ccgccaggct gcgtgatgcc ctcggcgtac ggctaccaga
    13621 gtcattgcca gagatttatt tacatagagt cagttacccg cttcgcgacc tctttctgcg
    13681 ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt ttagtctcgg
    13741 tattgccatt gtcgaagagc agcttcagca actgcgtgaa cagggtctgg tgatgaatct
    13801 gcaacaaaac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc gctcgctgca
    13861 agccgccaga gaagcgacgc gtcccgttgc agccacgacc tatgcgcgat tgctgctgga
    13921 acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct caacatctcc
    13981 aggcgtttat gagggcgtag atggcgtgat gcgggtgatc gaacagcttg ccggagtcgg
    14041 tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg actattcgcc
    14101 agaaatgctc gatgaattac tggcaaccgg agcggttatc tggtcgggac aaaaaaaagc
    14161 tgggtgacga tgacggcctg gtggcactgc atctacagaa atatgccgca gaatcgttca
    14221 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca gcaagcgata atcgctgttc
    14281 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gataaaatcg
    14341 tcgaatcggt tgattcctcg gcactgcaag aggcgttatg ggcgctggtc tggcaaggcg
    14401 tcattaccag cgatatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
    14461 gcacctcaac tcgccgcagt caccgggctc gtcgtgggcg tcctgtctat gcacaacccg
    14521 tctcgccgcg ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
    14581 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
    14641 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
    14701 tgcaaacact ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
    14761 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgattg cgcgatctgg
    14821 cgacacaagc cacgcaaaca cgccactata caccagtggc gctctccgcc aacgatccgg
    14881 ctaatgtgtg gggaaatctt ctgccctggc ctgcacatcc ggcaacgctg gtaccaacgc
    14941 gtcgggcggg tgcgctggtg gtcgtttctg gcggcaaatt gttactctat ctggcgcaag
    15001 gcggcaaaaa aatgctggtc tggcaggaaa aggaggaatt actcgcccca gagattttcc
    15061 aggcgctgac caccgcactg cgtcgcgaac caaggctgcg ctttacgcta acagaagtga
    15121 atgatcttcc ggtccggcaa acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa
    15181 gttcgccaca agggctggat tggggatagt tgacatcctc cacgccctga aagacgtggt
    15241 tccctgctac gttcaagctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
    15301 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
    15361 agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga atttactttg
    15421 tgacaggcgg ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
    15481 tggcggaaca gcaagcacct gaccgccact ccagagctgc ttgtactcaa gctggcggcg
    15541 catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt
    15601 gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc
    15661 ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc
    15721 cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
    15781 gttgttgctg aatttgacct tccggcttaa ctgtcgctga agtctcgcca gcgtcttctg
    15841 gtttttttga aaactgttta caggctcaaa gactgtgcca tctgacagcg tggcgagttt
    15901 agccacgcca gcatccagcc cgatcattga tgctgaaggg tgaaccggag ttgatacttc
    15961 actttctgtc tgaatactga tgtaccacgt accgcaggac tggctgacag tgacattttt
    16021 cacaactccc gtgacctgac ggctattccg gtagcgcatc cagccaagtt tcggcagaaa
    16081 aatacggctg ttttcctgat cgagcttaac gtcctgcggg tagcggaatg cagcattctg
    16141 tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg
    16201 ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
    16261 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
    16321 attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
    16381 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca cctggtctta actggaattt
    16441 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
    16501 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa
    16561 ataccgacgc cagatttttg accatgacac gacagaaaaa ctacgcactt acttttcaaa
    16621 tgtatgtgct gattttgcag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca
    16681 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
    16741 catatcgaat aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
    16801 aggcgttttg tggagtcctg gctattttgc cagtagctgc gaaggtgcgc caatatccgt
    16861 catccgccag tacattgaac aacagcaaac acctggtcag gtggaaaacc gcgccttata
    16921 tccccgccct gaaggacgag gttttacgac gcaccggata aaggactgac ggatgcccgt
    16981 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
    17041 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
    17101 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
    17161 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
    17221 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
    17281 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
    17341 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
    17401 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
    17461 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
    17521 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
    17581 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
    17641 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
    17701 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
    17761 ggcccatgct tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc
    17821 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc agaaaacagc
    17881 gaaaaaagcc gccagtaaaa gtatgaccaa aagcaaaacc gcttcttctg ttaaaaaatc
    17941 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
    18001 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
    18061 agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg tgcaaaaagc
    18121 cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggcgg
    18181 cagctcaccg cataccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
    18241 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcaggacc
    18301 tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
    18361 tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
    18421 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
    18481 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
    18541 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
    18601 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
    18661 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
    18721 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
    18781 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
    18841 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
    18901 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
    18961 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
    19021 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
    19081 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
    19141 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
    19201 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
    19261 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
    19321 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
    19381 agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
    19441 tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag cggaaattac
    19501 cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
    19561 tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta acggaggtac
    19621 aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
    19681 ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
    19741 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
    19801 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
    19861 taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta ccgtcgtcag
    19921 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
    19981 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
    20041 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
    20101 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
    20161 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
    20221 gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg gcgcgatggc
    20281 ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
    20341 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
    20401 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
    20461 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
    20521 caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac gcgatattca
    20581 ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa aagagaaaac
    20641 acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg ctccctcgag
    20701 aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga ggacgataat
    20761 cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt ttttattacc
    20821 acacaaaaag tgatattacg catttttaca cactgtgatg aaaaaatctc ccgtcattta
    20881 taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt
    20941 acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt tttccggcgt
    21001 accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg
    21061 caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag atgtagcgaa
    21121 acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc
    21181 tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact cccctagcgc
    21241 ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg cgaccagcag
    21301 cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct tccagaaagg
    21361 ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg cttatctgtc
    21421 gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt acccagagcc
    21481 gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg actggggtga
    21541 agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg gctacatggc
    21601 cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg gcccgctgga
    21661 acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag aagcgatgat
    21721 caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc
    21781 catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg gtggcgatat
    21841 catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc cgcaggatgt
    21901 ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg cgctgaccac
    21961 gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt tggatcgtat
    22021 cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga ttgaacgccg
    22081 ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc
    22141 ttccggctcc cgtagccact ccttattcag cgtctcacta tcgccgagat actcaagcag
    22201 ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg ccgaatccgg
    22261 gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg gtttggcgaa
    22321 atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg atgattccca
    22381 gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt taggtagcgt
    22441 tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg
    22501 gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc ttagcatccc
    22561 catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc caattgccag
    22621 ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct gaaagacaag
    22681 aagttcgaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg tacgttctgg
    22741 cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg
    22801 acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt tgagaaacca
    22861 cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg gtaccgccag
    22921 ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacacgat gcagctcctg
    22981 tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga gtgaatattc
    23041 tgaccacata gtctgcctgc aaaatttttg aaaccagtca tcaaatatta ccgtttcaca
    23101 acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt agagaatata
    23161 catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac tcggttttct
    23221 ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc aaacgcctgc
    23281 gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag cccagcttct
    23341 gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg gcctgacaat
    23401 ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc tgctggtatt
    23461 gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag cattagtgac
    23521 agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc cgctggtggg
    23581 tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt tttcctggca
    23641 ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta ttttctggct
    23701 caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta ccgacctgct
    23761 acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag ccagtttttt
    23821 tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc cggcagttat
    23881 tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg gctgtcgcgc
    23941 cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt
    24001 cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct
    24061 gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg ttgtcgccca
    24121 ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga acactctgca
    24181 actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta tcagcacgcc
    24241 attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc tgggttacat
    24301 gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag tcgctcatag
    24361 cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa attttgttgt
    24421 atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat aaatattatg
    24481 tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg caagggttcc
    24541 tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg agatttctca
    24601 caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag tgatggagaa
    24661 actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg gtaccgtatc
    24721 gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc ggcggatatt
    24781 cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt ggggttaggc
    24841 gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt tagcaaagtc
    24901 ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct gcgtattgcc
    24961 ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat
    25021 tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca atattcctgc
    25081 gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct caaaatgatt
    25141 tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg cggctggggc
    25201 ggactagcgc actacatggc atctaactat gacgtaagcg tggtcggcgt caccatttct
    25261 gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac cattttgctg
    25321 caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg gatgttcgag
    25381 cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg
    25441 gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc
    25501 tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca gattgctcaa
    25561 tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga ttacgatact
    25621 acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc ggataactac
    25681 agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg tgctttccgc
    25741 gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa cggccttcga
    25801 gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag atcaggcttc
    25861 tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta ccgtatctac
    25921 cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt ttttcttccc
    25981 aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg ttggcgtgac
    26041 ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca ggatatattt
    26101 catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg aggttaatat
    26161 tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc caatttcatc
    26221 actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat cgccaagatt
    26281 ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt taatttccgt
    26341 cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc cgtccagcat
    26401 gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag
    26461 ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa tcagttaaga
    26521 cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg gtaagaatgg
    26581 ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt tcccctgctt
    26641 cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac aaataaaagg
    26701 tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag caatcccggt
    26761 gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga tggcgggcgg
    26821 ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc ttccggcgat
    26881 cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat taaatggttc
    26941 cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg caggttttgt
    27001 ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct acatggaaaa
    27061 catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc
    27121 gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg caaaaaccaa
    27181 gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga actatatctt
    27241 tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg tggctactgc
    27301 ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg cccgctccat
    27361 gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgag cctgcggtta tgaaacgact
    27421 gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac tgtttgccgt
    27481 cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc agattgccct
    27541 gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg taactatccg
    27601 cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg cgcggaccgg
    27661 gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt cactgcggga
    27721 gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg cgcatttgat
    27781 gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca gtgggatttt
    27841 gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt actgggtgct
    27901 gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac ctatggggcc
    27961 agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta tgatgatgct
    28021 gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta
    28081 ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt
    28141 gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt ttgccgactg
    28201 gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt gatgaagtca
    28261 ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg caggcgtttc
    28321 actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc gccattcaga
    28381 ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc gtcgagaatc
    28441 aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc ataaccaata
    28501 atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct
    28561 tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac tgttccacct
    28621 tgtattaccg cattttccag cgtaccggca tgcacttcag cccgcccccc cacattcacc
    28681 gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc cagacgataa
    28741 atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt catggtgcca
    28801 ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc ctgattaatc
    28861 tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc atctaccacg
    28921 accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat gattttttcc
    28981 gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa accgcaggca
    29041 taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt agcgagcgta
    29101 gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc accgtcgctg
    29161 accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc ggttgccact
    29221 ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc ttcaacaatc
    29281 aactcgcctt cagtaagttg cgtcgcgcca aggacctcgc cggtcttaac aatttgcaca
    29341 ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt tgctgcatct
    29401 gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac gacagatttg
    29461 agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt tgtatcagat
    29521 gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag ccaatttgct
    29581 taaaagctct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc ttgccacctt
    29641 tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt catagctcag
    29701 ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac
    29761 catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg gggtcggtgg
    29821 ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt ttccctctgt
    29881 ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag
    29941 gagaatcgct atggcaactt tgttacaact tcattttgct tttaatggcc catttggcga
    30001 cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac ctggttttct
    30061 gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct accttttcac
    30121 tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga aaaatctcgg
    30181 cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc aaatcaatca
    30241 ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc atccctgtct
    30301 gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta aattcgaaag
    30361 aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg gcctacgggg
    30421 atctacacct tattctcgct tctacagcaa caaactccac tttctatttc tatcttcgag
    30481 caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc aaaaatgatg
    30541 ctggcaaata ttgccagtat tgaaataccg ccgattaatt gtacgtatct cgaatggcta
    30601 caaaagcaag aagccagcca tctccagcgt tatggcgtca aaaaagaaac cttgcacgat
    30661 cgtcagtttt taccgcgaat tctgctgggc gaatatttcc gcgatcaatt tttacgatta
    30721 gtagaccagg cacgacagca aaaatttgca gtggctgttt atgaatcatg ccaggttacc
    30781 gatctgcaaa ttacaaatgc tggcgtcatg ctcgctacaa atcaggattt acccagcgag
    30841 acgtttgatt tagcggtgat tgccacgggt cacgtctggc ctgatgaaga agaagcaacc
    30901 agaacgtatt ttcccagccc gtggtcaggc ctgatggaag caaaggtcga tgcgtgtaac
    30961 gtgggtatta tgggaacatc cttgagcgga ctggatgcgg caatggcagt ggctattcag
    31021 catggttcgt tcattgaaga tgataaacaa cacgtcgttt ttcaccgcga taacgcaagt
    31081 gaaaagctaa atattacgtt gatgtcgcgc acgggtattt tacccgaagc cgatttctat
    31141 tgccctattc cctacgagcc cttacacatc gtcaccgatc aggcattaaa tgctgagatt
    31201 caaaaaggtg aagagggcct tttggatcgg gtatttagat tgatagtaga ggaaatcaag
    31261 tttgctgatc cagactggag ccaacgcata gccttagaga gcctgaatgt cgattccttt
    31321 gctcaagcct ggtttgccga gcgcaaacaa cgcgacccat ttgactgggc agaaaaaaat
    31381 ctccaggaag tcgaacgcaa taaacgagaa aaacatactg ttccctggcg ttatgtcatt
    31441 ctgcgcctgc atgaagccgt acaggaaatt gttcctcatc tgaatgaaca cgaccataaa
    31501 cggttcagta aaggccttgc cagggttttc atcgataatt atgcggcaat cccttcagag
    31561 tctattcgtc gactactggc cttacgtgaa gcggggatca ttcatattct cgccctcggt
    31621 gaagactacg aaatggaaat taatgagtcg cgcaccgtcc tgaaaacgga agacaacagc
    31681 tactcgtttg acgtttttat tgatgcccgc ggacagcgtc cgcttaaagt gaaagatatt
    31741 cctttccctg ggctacgcga gcaattacag aaaacagggg atgaaatccc tgatgttggc
    31801 gaagattata cgttacagca acccgaagat attcgtggac gcgtagcgtt cggcgcgttg
    31861 ccctggttga tgcacgacca gcctttcgtt cagggactta cggcatgtgc agaaattggt
    31921 gaggcgatgg ctcgggcggt cgtaaagcct gcatcccgtg ctcgtcggcg tctttcgttt
    31981 gattaaaggt aaagctatca gcactcaaca aactgatgct gttcattaag acgatagcgg
    32041 actccggttc gaatattgtt ttcaccttca atggcgacgg cgaagcgaac tctttcgccg
    32101 tcatgatatg ccagtgcggc gcttccccct agcccgagaa taattgagtc gaccacgccc
    32161 gtgctcacta ccgttgaatt ttcaccgctg gcaatgatgt gtacattgtc gccgctgttg
    32221 gcaattctgg cgtttgcgcc aaagctggcg atttgcacca ggtcaccatt actggcgaca
    32281 tgacaccgtt cacccaatgt acaaacgcgt actcgcattc cggtattcgc aatccgacta
    32341 ctatctccag cgctgcttat ccgcgaagaa ttaccggtta cagcaacccg tgcgcgttca
    32401 ccggagctgc caatgtgact gttaaagccg acacttccaa tccgcacgct ataacccata
    32461 ctggcaattt gcgcggcata cccagcggtt gcgattcggg cgttatcctc acaacagccg
    32521 atgcgagcat gatcgtcgct gcgggcaaac tgactgcaaa atggtatctc tccggggtct
    32581 gtggccagtt tttgcataga ggaaacttcc tgatgcgcga agttttcatc cgccagccat
    32641 tttttccacg catactcaac cagactctcc gcccagtccg tatatccttc ctcaatcagt
    32701 gcatgacgaa tatcggcata actaccgccg cgtgggaaat ggcgcaggaa ccagcgatac
    32761 atcaccgctc cgatacgcca ttctcgcagg tctgctcgtg taatgatcat tcgttagtgc
    32821 cggtgatagc catcgaccat gcttttaaag gtttcgtgtg gcgtacgccc cgtggtgcaa
    32881 agataaagat gaccgaagat aaaaaagagg cttataaatg ccagagcaaa atgcgcctgc
    32941 aataaccagt atcttacgcc aggaaacaca tctcccacgg cttgcggata gagacacaac
    33001 agccccgtca atagtaacaa cggcagcaat ccatacatga caccaacata ggcgacctgc
    33061 tgtaagggat taaatttaga ctgtgttgtt gccgggaaag gatgttcttc cccctgcata
    33121 atgccaaaca aataaaatcg cgtttgtttt gccgctcgtt ccagccaccc ctgacgacga
    33181 atgcgatagt ggtgaccatt atccccaacg gcattgatca gcacaaagcc gagccagcat
    33241 gccagtaaca aaaatccgca aacttcatgc accgcaacca gacttttaac cgcagttgcg
    33301 cccaccatcg caaagtgatt tatcagccca cttgccagca acaatacaaa gagtaacgca
    33361 ttcgaccagt gccataatct gaccgctttg ctgtaaagat aaaccttctc accatgatcg
    33421 gtggctgatt tcttcacgcc acgagcacga agcaaggcgt gcagccccag caccaaccac
    33481 atcccaacca gcaatactcc ggcaatgatc aaccacaccg gccaaaattc gggagtgaac
    33541 tgtggcacat agttcgctaa ctggctctga aactgttcag catgttgcga cgggttcata
    33601 cattttcctt tttaatcaaa tgttgaccga accggcgata cagatgcggt tttccggcac
    33661 caggtagctg atattgatag tatttattat cctgcaacca cgcctgaatt tctgggctat
    33721 cttcgcgacc aaagatcaat gcgtgttccg gacaggcgct gacgcaaatg ggcggaaagc
    33781 cttttgccag tcgggactca gcgcagaaat cacatttatc tgccacttta gtgacagggt
    33841 taaggtagcg aacctggtac ggacaagcgc caatgcaata actgcagccg atgcactgcg
    33901 atttttctac ccgcacaatc ccctgctcat cacgccacga tgcccccgtc gggcatacgt
    33961 cgatgcacgg agcatcttcg cagtgctgac atgactggcg aaaaaagtga tactgcgttt
    34021 cgttgtcgtt atcggtgact ggaatgtgtg ctatcgataa acggcttccc tgagcgggga
    34081 cgtggttagt tttacggcat gcgcgggcgc aaatattaca gccattgcac cgtgactcgt
    34141 catgaatcat ggcataccga acttcctttt cttgcctcgt gttcgctaac agagaagacg
    34201 cagaaccagt aaaaaagatt actgttccca tccccagaac aaatttgcgt cgagtaaagg
    34261 acatctgatt atcccctaat gcagtggctg caactgtcca ccctatccat gacatctccc
    34321 tgaaatttca ggggttctga aactgctcta cctcgaacca ttcgcataag ccataagcct
    34381 tgccataggt cataaatcgc tcgctaaaca agtacaacac cgccgtcgat gactccacct
    34441 gccgtatatc ggcatattca ggtgcaacat ccatcgcggc gatcgcggct tcaatctgcg
    34501 cttcctggaa gtaatatggc gcttgcatca gcatggcgac tttgtaagga cgaggatacg
    34561 tctggcactc aaaacgaacg gcgtgtgcaa tggcgcgaca aatgtcctgc tccacaactt
    34621 gcaaagacat cgccgcgtag ttttcactca tggcctgcgt tgagtaatag taatcatcct
    34681 gacttcccgt catacaagcg atatccgcgt agtctccgtt ctgcttaatg ctttccagca
    34741 aaactgacag ttcctcttct gcaaccgaat agggtggcga gagaaacacg gtgcgcgcca
    34801 ccagctgccc agccgcagaa tgctggcgaa tgaactcagc caggatttca gcaggttctg
    34861 gttcggttac cgtatcaacg gggtgacgca tcccctgcaa ccattgccgc ttcgcctcgt
    34921 caacttcgct gactttcgct aacggcaatt catcctgatg gttcatctta cccattcctt
    34981 acgcaggcag tagattgtgt gctgccagat cggcggcaat atcttccagc cccagacgct
    35041 gcaatgtttc gcgggttgga caaccaagct gtgggtccca gcccatttct ttgtagaaca
    35101 tcgtgagcga agcatgcata tcgtcacgat ccattttgtc agtaccttca gtaaagaccg
    35161 ggatctgcgg atccttgtcg aaaacccagg aacagataag atcgtgttca ttacgcatat
    35221 ctttggtttg catcggtttt accgtgtagg cacgatgcaa cgtaaagata cgctctgcag
    35281 ctaaatctaa tttttcctga gtcatctctt cgccggtgat cgctttgaag aatttggctt
    35341 aaagcgccag atcgccccgg taattacggc ttttcagcgg cgaaacggtc attggccaga
    35401 cccagttgca cagagtgacg gcgttatgca aacagacccg caacagcgac cactttgcat
    35461 atttaatttt tgcgtcattg attggcgtgt agtttttggt ttcatcgtaa gcatcttcag
    35521 aaccaaaaag ttctttcgcc acttcacgtt gcagtttcaa tggcaagccg gaaccaataa
    35581 agttgatatg ggtatgcgtc atgcagtcac ggttgaacat acagttaaca atggaaccga
    35641 cctgcgctga cgcttcattg gcatggtgaa ccggatagcc aaacggcgac cagagtttat
    35701 ttttcgcgta gccccagtac tctttaccca aattccagcg ttctgcgatg gcatatgaac
    35761 catcagccag gtgactcagc tcacccacac gatgcgccag acggtagtaa aaatctttaa
    35821 tgaagttaac gtcacccgct tccagttgat cccagcgaat ttctgcatac tcttcagctg
    35881 gcagaacacg cttgaacaca cctttgctgt aacaataagt aaagtcgcga tgcaactgcc
    35941 cgtagttaca ccataggccg tagtcatcga acaaattcag accaaccagg ttaccaatca
    36001 cacgaccatc gtctttatct tcaaaatctt tcggcccgtt cgggaagatg gtggtatgga
    36061 caaagtttgc tacacaagtg ttaccacctg tgctgggcac gccaaactct ttcacccgag
    36121 gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccgctc atttttactg
    36181 tgtatttctc tgccgccggt cctaagtcaa aaacggattt ataggtacga aagccgaccg
    36241 tattctgatt gcctggcgga atttcacccg tttcaatcgg accaccttca gccgcgcccc
    36301 aaaacagccc tttacgtgct gtccagcgtg acttgggatc tgaatactct gcccacgatt
    36361 gtggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc atcatgtaat
    36421 cattgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc ccttcaaccg
    36481 caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg ccgctgtggt
    36541 tacggctatt caacatgcca gagagaggaa taaggttttc cccagcctga ccaatagccg
    36601 ccacacaggt ttccgggctg gtcaatcgac aaatttcttc cgtcgttgcg cgcgtccctt
    36661 ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc ttcagccata
    36721 ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat ttcatttgcg
    36781 ctgcaaaaaa gccacccata tgggcatcgc gactaaattt cctttggtaa aagtagaaag
    36841 tgaggtgata tttacgcgag aactacaggg ggcaccagat ccagttaatg ggccggtagc
    36901 aaagactaat ttattcgctt catcgaaagg tttcgtgcct agcggtactt cgtcatacat
    36961 aattttgtag ccgaagccca tgccaccgac aaaactttta aacttactgg aatcttcgag
    37021 ggtaatattt cctgtcgtga gattgactct taatatatta cctgtccaac cgttagccat
    37081 gattttttcc tttgcaagat tcacacagta atatctttcc actcgataat ttttaacgcc
    37141 cctgttgggc aggcgtttgc gcattcaccg cataacacac atttcgagga ttttttactt
    37201 tcggtattta cggtggccat catccacggg catgccgtag tacaggcgct acagccaata
    37261 caacgtttat gatcgacggt aatacagcct tctttctgct gccaggtaat cgcaccaatc
    37321 gggcagacgt tcatgcattg cggctctttg cattgacggc aggtgtccgc ggtatagttg
    37381 agatcgccat acaggccgcc gccagagcca accccgttgt cgccaaagaa ataattgcga
    37441 tggattttga tacgggagaa gaatgtccct actgagccat cgttgaagtt ggtacaagag
    37501 atttcacagc ggtggcaacc cgtgcatcgc gctcgctggg tcaccagcac ccctttgggt
    37561 gtatttatca accctactgt gccgctatca atatcttcct gtttgcaacc caaaagtgac
    37621 agtaacgcag gggcaattgt tagaccagca aggccttttc ctgatatacg caagaattct
    37681 aatcgcgtta agccaatatc taatagtgga cgatcaaccg ggttcattta tttcgcatcc
    37741 tcttcgcaaa cacagtgctg acaattcagc agcctgatat taagcattca actaatacgc
    37801 tgctgactct gtccccaaca ccagatattt tccctaaccc gatagtgtat gagaaaagat
    37861 ttaacgccga taaactaatc ctttatggtt aggagaaaaa ataatgtgat tattctgagc
    37921 ccttaacatt gatcgttatc aattaaaatt acaaagcaag aaattataag cgaactgata
    37981 tttattatca tttgaaataa atttaactta agcgacaatc atttgtgacg ttcattgcag
    38041 aaaataaaaa taacattatc gctataaatt aaaatacata ccgaaattcg tataatttca
    38101 gcagttgtta tttagaatga ttaactggac cgcaactgaa gcaagaatat caggatgaag
    38161 cttaccgcct catcctgaat ggtcatttta ccagtgcgtt aatgacggtt tccatcgcct
    38221 gcttttccag ctcactgcgc tgaatgcgtt tattggctaa tgctgtacgt aatacgcctg
    38281 cgataacaat atgctttggt tcctgcccca gatcgcgcat ttccagcaca actttaccga
    38341 caactctgca catttctcgg tacagttcat cctcttttgt ccgatttccc attctttata
    38401 cctatttatc atgccaacta tcaggtaatg ctgccaactt actgatttag tgtatgatgg
    38461 tgattttaag gtgcttgcgt ggcttccatt tccatcagat gtccttcctg ctccgctact
    38521 gaaggcgtgg t
//
LOCUS       DAEEDS010000032        37790 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEEDS010000032 DAEEDS010000000
VERSION     DAEEDS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37790
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.032"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..177)
                     /locus_tag="KLH32_001810"
     CDS             complement(<1..177)
                     /locus_tag="KLH32_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBE0138661.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(261..1145)
                     /gene="yhdJ"
                     /locus_tag="KLH32_001811"
     CDS             complement(261..1145)
                     /gene="yhdJ"
                     /locus_tag="KLH32_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE0138662.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1231..1527)
                     /gene="fis"
                     /locus_tag="KLH32_001812"
     CDS             complement(1231..1527)
                     /gene="fis"
                     /locus_tag="KLH32_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBE0138663.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1553..2518)
                     /gene="dusB"
                     /locus_tag="KLH32_001813"
     CDS             complement(1553..2518)
                     /gene="dusB"
                     /locus_tag="KLH32_001813"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBE0138664.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2847..3728)
                     /gene="prmA"
                     /locus_tag="KLH32_001814"
     CDS             complement(2847..3728)
                     /gene="prmA"
                     /locus_tag="KLH32_001814"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBE0138665.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3740..5191)
                     /gene="panF"
                     /locus_tag="KLH32_001815"
     CDS             complement(3740..5191)
                     /gene="panF"
                     /locus_tag="KLH32_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBE0138666.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5181..5423)
                     /locus_tag="KLH32_001816"
     CDS             complement(5181..5423)
                     /locus_tag="KLH32_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBE0138667.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5532..6881)
                     /gene="accC"
                     /locus_tag="KLH32_001817"
     CDS             complement(5532..6881)
                     /gene="accC"
                     /locus_tag="KLH32_001817"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBE0138668.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6892..7362)
                     /gene="accB"
                     /locus_tag="KLH32_001818"
                     /gene_synonym="fabE"
     CDS             complement(6892..7362)
                     /gene="accB"
                     /locus_tag="KLH32_001818"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBE0138669.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8340..9314)
                     /locus_tag="KLH32_001819"
     CDS             complement(8340..9314)
                     /locus_tag="KLH32_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0138670.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9466..11406
                     /gene="csrD"
                     /locus_tag="KLH32_001820"
     CDS             9466..11406
                     /gene="csrD"
                     /locus_tag="KLH32_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBE0138671.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11711..12754
                     /gene="mreB"
                     /locus_tag="KLH32_001821"
     CDS             11711..12754
                     /gene="mreB"
                     /locus_tag="KLH32_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBE0138672.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12820..13923
                     /gene="mreC"
                     /locus_tag="KLH32_001822"
     CDS             12820..13923
                     /gene="mreC"
                     /locus_tag="KLH32_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBE0138673.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13923..14411
                     /gene="mreD"
                     /locus_tag="KLH32_001823"
     CDS             13923..14411
                     /gene="mreD"
                     /locus_tag="KLH32_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBE0138674.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14420..15013
                     /gene="yhdE"
                     /locus_tag="KLH32_001824"
     CDS             14420..15013
                     /gene="yhdE"
                     /locus_tag="KLH32_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBE0138675.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15003..16472
                     /gene="rng"
                     /locus_tag="KLH32_001825"
     CDS             15003..16472
                     /gene="rng"
                     /locus_tag="KLH32_001825"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBE0138676.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16540..20340
                     /gene="yhdP"
                     /locus_tag="KLH32_001826"
     CDS             16540..20340
                     /gene="yhdP"
                     /locus_tag="KLH32_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBE0138677.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20496..21941
                     /gene="tldD"
                     /locus_tag="KLH32_001827"
     CDS             20496..21941
                     /gene="tldD"
                     /locus_tag="KLH32_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBE0138678.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22069..22998)
                     /gene="aaeR"
                     /locus_tag="KLH32_001828"
     CDS             complement(22069..22998)
                     /gene="aaeR"
                     /locus_tag="KLH32_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBE0138679.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23181..23384
                     /locus_tag="KLH32_001829"
     CDS             23181..23384
                     /locus_tag="KLH32_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBE0138680.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23392..24324
                     /gene="aaeA"
                     /locus_tag="KLH32_001830"
     CDS             23392..24324
                     /gene="aaeA"
                     /locus_tag="KLH32_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBE0138681.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24330..26297
                     /gene="aaeB"
                     /locus_tag="KLH32_001831"
     CDS             24330..26297
                     /gene="aaeB"
                     /locus_tag="KLH32_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBE0138682.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26389..26661
                     /locus_tag="KLH32_001832"
     CDS             26389..26661
                     /locus_tag="KLH32_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBE0138683.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26717..26980)
                     /gene="yhcN"
                     /locus_tag="KLH32_001833"
     CDS             complement(26717..26980)
                     /gene="yhcN"
                     /locus_tag="KLH32_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBE0138684.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27345..27815)
                     /gene="argR"
                     /locus_tag="KLH32_001834"
     CDS             complement(27345..27815)
                     /gene="argR"
                     /locus_tag="KLH32_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBE0138685.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28250..29188
                     /gene="mdh"
                     /locus_tag="KLH32_001835"
     CDS             28250..29188
                     /gene="mdh"
                     /locus_tag="KLH32_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE0138686.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29250..30317)
                     /gene="degS"
                     /locus_tag="KLH32_001836"
     CDS             complement(29250..30317)
                     /gene="degS"
                     /locus_tag="KLH32_001836"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBE0138687.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30407..31754)
                     /gene="degQ"
                     /locus_tag="KLH32_001837"
                     /pseudo
     CDS             complement(30407..31754)
                     /gene="degQ"
                     /locus_tag="KLH32_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31908..32306)
                     /locus_tag="KLH32_001838"
     CDS             complement(31908..32306)
                     /locus_tag="KLH32_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBE0138688.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32500..33627
                     /gene="zapE"
                     /locus_tag="KLH32_001839"
     CDS             32500..33627
                     /gene="zapE"
                     /locus_tag="KLH32_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBE0138689.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33847..34275
                     /gene="rplM"
                     /locus_tag="KLH32_001840"
     CDS             33847..34275
                     /gene="rplM"
                     /locus_tag="KLH32_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBE0138690.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34291..34683
                     /gene="rpsI"
                     /locus_tag="KLH32_001841"
     CDS             34291..34683
                     /gene="rpsI"
                     /locus_tag="KLH32_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBE0138691.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35078..35716
                     /gene="sspA"
                     /locus_tag="KLH32_001842"
     CDS             35078..35716
                     /gene="sspA"
                     /locus_tag="KLH32_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBE0138692.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35722..36219
                     /gene="sspB"
                     /locus_tag="KLH32_001843"
     CDS             35722..36219
                     /gene="sspB"
                     /locus_tag="KLH32_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBE0138693.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVCFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36262..37629)
                     /gene="dcuC"
                     /locus_tag="KLH32_001844"
     CDS             complement(36262..37629)
                     /gene="dcuC"
                     /locus_tag="KLH32_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE0138694.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(37650..>37790)
                     /locus_tag="KLH32_001845"
     CDS             complement(37650..>37790)
                     /locus_tag="KLH32_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0138695.1"
                     /translation="TQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKH
                     YQ"
BASE COUNT         9152 a         9455 c         9932 g         9251 t
ORIGIN      
        1 cattctgtcc tgaaaattca gttcgtcaca gttaaccagt tttagtggat taactgagtt
       61 catattacgg catttgtctg tttctgttgt cagcagttca atccactgca tcagtaaagt
      121 atcaaacaga aaaacgaaga cagcgaaaac gacgagccac caatacttgc gaatcattac
      181 ctgagcctgt gcatgtttaa ccaagtctgg caggaaatat acacgaaatg aataatctga
      241 aaactaggct tacacttcct ttactttgca atgagatcgg ggtcaacttc gcttaaccgg
      301 cttcgttttg acaggttgcc ggtctttctt tttttcactt tcgccagttc ttccgcggag
      361 taatgcgacg cgacatccag ccgtcgaagc cccattttga tgtactcgct gttgatctca
      421 ataccaatga attttcgtcc gctggcgatg gctacggcac cggtagtaaa gctaccggca
      481 aacgggtcga gaacgatatc gcctgggttg gaagaggcga gaataatgcg tttcagtaag
      541 gctttcggtt tttgcgtcgg gtggttctca tattcatcca tcaaataacg cacgcgcgga
      601 aaatcccaga cgttacccgg tactttttga tgattgtatg gctgtggagg atttttgcga
      661 taatcgatca acgcgcgctg cgatccggtt ttggcttcta ccagaatagc atcaccgttg
      721 aatgtgtagt tctttgcgtc tttcaccatc atcaggatgg gttcgtacat ggagccgtag
      781 tgttttttcg cctgtactcc agaactgtca tatgaccaga cgatgcgact tttgatggta
      841 aaaagcttgc ggcactggag atcgataaag ggcatgtttt ccgtactgtt cataatgtac
      901 atgctgccct gctttttcag aacgcggtgg cactctgcaa tcacttcaaa cagccagtcg
      961 ataaacagat cttctttcca ggcttcgatc agaccatcaa aatttttacc gatgttatac
     1021 ggtgggtcgg caaagagcag atcgacgctt tcagtaggta gctttttaag ttcggcaaag
     1081 gcatcaccgt gaataatggt cttagcttca ttaccaaacc gggtcggttc acatcctgtt
     1141 ctcatggtca ctccctttgt gacacctata aaaaaggcgc ttccccatac cgagtagcgc
     1201 ctttttaatc aagcatttag ctaacctgaa ttagttcatg ccgtattttt tcaatttttt
     1261 acgcagcgta ccacggttga tgcccatcat cagcgcagca cgggtctggt taccacgggt
     1321 gtattgcatc accatgtcca acaggggctg ttctacttca gccagtacca gctcatagag
     1381 gtcattcaca tcctgaccat tcagttgagc aaaatagttc ttcagtgcct gtttaaccga
     1441 gtcacgcagg ggtttttggg ttacctgatc ctgagagtta acggtagaaa cggtcagtac
     1501 gtcagaattt acgcgttgtt cgaacatagt tctgtcagct ctttatttct gtttacgcaa
     1561 aattttcgaa gtatgcctcc aacgcctcca gctgttcgct ggcatcctca atggcgttga
     1621 atgtgcgccg aaactggtca tttggagcgt gttcctggag ataccaggaa acgtgtttac
     1681 gtgcaattcg gtaccctttt gccggaccat aaaagtcatg cagttcccga acgtgcgcgc
     1741 aaagcaagcg cttaacctct gccaaaggca gtgggggcag caactcccca gtgtccagat
     1801 aatgctggat ttcccgaaag atccagggtc ttccctgagc tgcgcggcct atcatcaggg
     1861 catccgcccc tgtatagtcg agcacagctc tggctttaag cgggtcagta atgtcgccat
     1921 tcgcgataac cggaatggaa actttctgct taactgcccg aatactgtcg tactcagctt
     1981 ctccattgaa caaacaggcg cgtgtacggc catgaatggt cagagcctga atgccacagt
     2041 cttcagccag ttgggcaatc tcttcgcagt tacggtgttc cggtgcccag ccggtgcgaa
     2101 tcttcagggt aacaggaacg tccactgcat tgacgacctc ggtaaggatc gatttaacga
     2161 catccgggta ctgcaagagg gctgaacctg cgagcttgcg attcactttt ttagccgggc
     2221 aacccatatt gatatcaata atctgggcac cgctttccac gttaatacgt gctgcatctg
     2281 ccatttcttt cggagcgcta ccagcaattt gcacggtgcg aataccgggt tcatcaatgt
     2341 gcaccatccg taaacgagat ttgtcgcttt cccaaacctg tgggttagaa gacatcatct
     2401 cggatactgt caatccggct cccatctcgt agcacaacgt ccgaaaaggt ctgtctgtaa
     2461 tgccagccat gggcgctgcg atcaggcgat ttctgagctg atattgtccg atgcgcatga
     2521 gttaagaaat gaccatactg tgactgcaag gcggcgtata ttacgcattt tttgcacgag
     2581 atgaaaggcc aaactttgac caatcctctg agatggatca aagaattgca tttaaaatga
     2641 gcgtggtgcg ataattactc ataaaaatca tcatattaga aaatagtgac taaaatttac
     2701 actcaaagaa atttgagtaa gttctcaatt tttctttatg aatgaaaatt ttggcacgca
     2761 aatttgcgta aataatcggc aaatttacgt gccttttgtg agcttgctcg cacttcgccc
     2821 cgcgtcaccc tacggcgatg cgaaggtcaa ttcttacgac cggtaatacg gcaccactct
     2881 tctttttcca cgaccgggtc cagtgcgaag ctatcggcat aagcttcaca aacgctctct
     2941 gcctggcttg ccagaatacc ggaaaggccc agcaaaccgc ctgaaaccgg caggacgctg
     3001 attaacggtg ccagttcacg taatgggcct gcaaggatgt tagcgaccac cacgtcggct
     3061 ttcatttctt ctggctgatc tttcggtaag tagagttcca gacggtcaga aacgccatta
     3121 cgttcggcgt tatcgcggct ggcctgaatc gcctgcggat cgatatcaat accaatggct
     3181 tttgctgcac ccagtttcag cgccgcgatc gccagaatgc cggaaccaca gccaaagtcg
     3241 atgactgttt taccggttaa atcgaggctg tcgagccatt gcaggcacag agaggtggtt
     3301 ggatgggtac ccgtaccaaa cgccagccct ggatctaaca tcacgttgac ggcgttttcg
     3361 tccggcacat cacgccagct agggcagatc cacagtcgtt caccaaagcg catcgggtgg
     3421 aaattatcca tccattcgcg ctcccagtct ttatcttcta gttgttcgat tttatgcgcg
     3481 aagcctgcgc cgagcagcgg atggttttcc agaatcgcca ccacgtcgtt catatcggtt
     3541 tcagcgtcga acagaccaat cacatcggtg tcgccccaca ggcgcgtttc gcccggcagc
     3601 ggctcaaata ctggcgtatc gtgggtatcc tgaaaagtga tagaaacggc acccgcttcc
     3661 atcagcgcat cgctaagatc ttccgcgttc gcgccggtgg tgttcagttt cagttggatc
     3721 caaggcatgg caaaactctt tatttatcag tagtcaaaac ggtagcttgc gggacggatg
     3781 taccgaaacg gtttccgacc aggaaagcca gcaaacttag tagtaacgag ggcacgatag
     3841 ggtggaagcc caggtactga atattcagcg tcgcgagtac ggcatacagc acgccgccaa
     3901 cgatcatcgc acttagcgcg cctttggcgt tggcgcgttc ccagtaaaga cccagcacca
     3961 gcggccacag gaaaacggct tccagcccac cgaaggccag caaattcagc cagatgatca
     4021 tttctggcgg cttccaggcg gcaagcagca gcaacgcgcc gagaactaac gtaattaccg
     4081 ccgacatccg cttcagacgc gtctcgtttt gcatttgatc cggacggata ttcagataga
     4141 gatctttaat gatcgtagcg gaactttgca gcagttgggc gttaattgtc gacatgatcg
     4201 cagccatagg tgcagccaga aagatcccgg cagcaaaagg tggcagcact tttaccatta
     4261 acgtcgggat caccagatcc ggtacggtga gatcggggat cactgcacga cctaacgctc
     4321 cggccaggtg cataccgaac atcaggattg cgactataat cgtaccgatg atgatccccc
     4381 ggtgtactgc tttgctgtct ttataagaga tacagcgcac cgcagtatgc ggcaggccaa
     4441 tcacgccaaa gcacaccagt acccagaacg acgtcataaa ggcaggcgac agaatatcgt
     4501 cagcgccttg tggcgtaacc agttgcggat cgatggtttg caaggtctgt actgcgttgc
     4561 ttaagccgcc agcggcatgt accacgccaa taagcagcac aacggtgcca atcagcatca
     4621 caagcccttg catggtgtcg ttcagcacgc tggcgcgaaa gccaccaaag gcggtatata
     4681 acgcaatgct gataccaaaa atcagcagcc cggtttcata aggaataccc gccgcggttt
     4741 ccagcaggcg cgcaccgccg ataaactgca cggtcatcgc accaacgaac gcaaccagca
     4801 aactcaaact cgccagccac accagaagac gactctggta gcgggcaaac agcatatcgt
     4861 tcagcgtcac tgcattgtag cggcgcgcaa gaatcgcaaa cttcttgccg agaataccga
     4921 gtgaaagcca gacagcagga agctgaatca tcgccagcaa tacccagccc agcccgtatt
     4981 tataagcagc tcctggcccg ccgataaacg aactggcact gatataggtc gcggtgagcg
     5041 tcatcgccag cacaataccg cccatagagc ggctgccgag gaaatactca ttaaggaagg
     5101 tgccggtgct ccgtttacgc atcgcataaa ccgagatacc gaacaccacc accagatagg
     5161 cgaccagcgg tagaattact tcaagctgca tcgtcatcct ccagtgggat atcgcgatag
     5221 ataaatttca ccatcgccca gcacagtcca ataaacagta gcggcgtcag gatgcaggcc
     5281 atctcaaacc agcgcggaaa gccggtaaaa ccgggggcaa cgccaggtaa gtaagcgtct
     5341 actaaccaaa ctgccagata caaaagggtc agccccagcg cccagcgcgc ctctttatgg
     5401 gcctgaacaa aacgagtgtc cattttttgt ccctgatggg tgaagaaagc gaggattgta
     5461 ccttatgggg gttgtcgatc cccagtaata aaaaaggccg gaaaatccgg ccttttgacg
     5521 cttttgcagt cttatttttc ctgaagaccg agttttttct ccagatagtg gatgttagtg
     5581 ccaccatgct ggaagttctc gtcattcatg atgcggatct gcagatcaac gttggttttg
     5641 ataccgtcga tgatcagctc ctgcagcgca ttcttcatgc gggcaatcgc cacgtcacgg
     5701 ttttcaccgt agcaaatcag cttaccgatc attgagtcat agtacggcgg tacggtgtag
     5761 cccgcgtaga tatgagactc ccaacgtacg ccaaaaccgc caggtgcgtg gaaacgggtg
     5821 attttgcctg ggcttggcag gaaggtgttc ggatcttcgg cgttgatacg acattccacc
     5881 gcatggccgc gaacgtgaac ttcttcttgc ttgatcgaca gcggttgacc ggcagcgata
     5941 cgcagctgtt ctttgatcag gtcaacgccg gtgatcattt ctgtaaccgg gtgttctacc
     6001 tgaatacggg tgttcatttc gatgaaatag aactcgccgt tttcgaacag gaactcgaaa
     6061 gtacctgcac cgcgatagcc gatatcaaca cacgctttag cgcaacgttt gccgatgtag
     6121 cgacgcagtt ccggggtaat gcccggtgct ggtgcttctt cgaccacttt ctggtggcgg
     6181 cgctgcatgg agcagtcacg ttccgccaga tagatagcgt tgccctgacc gtcagccagt
     6241 acctgaatct cgacgtggcg aggattctcc aggtatttct ccatgtaaac catatcgttg
     6301 ctgaaagcag ctttcgcttc cgcacgggtc atggagatgg attgtgccag ttcagcgtcg
     6361 ccgcgcacta cgcgcatacc gcgaccgccg ccgccgccgg aggctttgat aatcaccgga
     6421 taaccaatgc gtttagcaat ggcgcggttt ttatccatat cgtcgcccag cgggccgtca
     6481 gaaccaggta cgcaaggaac gcccgctttt ttcatcgccg cgattgcgga tactttgtcg
     6541 cccatcaggc gaatggtttc tgctttcggg ccgatgaaga taaagccgga gcgttcaacc
     6601 tgctcggcaa agttggcgtt ctcggagagg aagccgtaac ccggatggat tgctactgcg
     6661 ccggtgattt cagcggcgct gatgattgcc gggatgttca gataactttt tactgacgga
     6721 gcagggccaa tacagaccgt ttcatctgcc agtaatacgt gttttagatc gcgatccgtg
     6781 ctggagtgca cagcgacagt cttgatgccc agttctttac aggcacgaag aatacgcaat
     6841 gcaatctcgc cgcggttggc aataacaatt ttatccagca tgttcgcctc gttactcgat
     6901 gacgaccagc ggctcgtcaa attctaccgg ttgtccactt tcgaccagaa ttgctttcac
     6961 ggtaccggat ttgtccgctt cgatctggtt catcattttc atggcttcaa cgatgcacag
     7021 ggtatcgccc acgttgactt tctgacccac ttcgatgaac gcttttgcgt ccgggcttgg
     7081 ggtgcggtag aaagtaccaa ccatcgggga acgtacgatg tgaccactga tttccgctgc
     7141 tgctggcgct tccatggaag gaacggtcgc cggagcggct gcgttagatt gagctggctg
     7201 ctgcatcatt ggtgcagcgt aagcttgttg catcacaggg aaacttgcgg caggagctgc
     7261 acggctaatg cgtactgact cttcgccttc agaaatttcc agttcggaga tgcctgattc
     7321 ttcaaccagc tcgatcagtt ttttaatctt acgaatatcc atgagtgggt tccgtactct
     7381 ttgtttagtg tgattgtgac aggcgtttca ccgccgtctg taaagcgtat gccccgacag
     7441 ctgtatgcat agcgataaat tccagcaggc cgggacgccg gtctattttg ccccagagtg
     7501 tcaatgttag acttgacgga cattgtgcag cgatctgctg ctatcctccg gcaaaaaaca
     7561 aaatatacct tcgttgcgcc aatgtcacct tttccgcagc gcaaaaactg cgtcagggag
     7621 gacgaggtcg cacattataa cgatttcgta gcaattggca gctaaatact ggtcttatca
     7681 gggaatataa tcaacagcta acatgtaaat aaccttcaac accgtgtaat ttgcaacaag
     7741 ccgcacaatt cacgaaatta gcgccatcat cgacggaact tcttataacg taaggtgaaa
     7801 agctgaaaag ccagccctgc gaagatgagg ggctgcaatg agataatctt cacagaccac
     7861 aaataatgta gggatgccag gatcgcgaca agatagacga cattacgcag cggttgctgg
     7921 ggattaccca attttcactg catcgtctgg atagatgtga atgctaaagc acgcaaaatc
     7981 aaccaactga caattctcag cattaaatag gttcgagtgg ttaatttgta gtcaaataaa
     8041 atcagattat tcaccccatg gtaccagaaa tcagtaacag gttaagcgta gtgtcgccca
     8101 ggcaaagcac cataaaccta acaggcgacg gaggcgtatc aataatggct gtttagagga
     8161 gtatcaatgc gcatcaattt gtggtcagtc gaaatcatga ctttccagtt tggatgatgt
     8221 tgtacaactt cctgaccaag gtgttcgacg aaagctgtcc ggctaaccgc ttttcccgtt
     8281 aaccgattga ataatgatgt caaaatgagt tccctctctt attattcctt gctaaatggt
     8341 tagttaacct tcaccagcgt gcgaccctgg atctggttat taatgatggc ctcggcaaag
     8401 ttcggtgcct ctgacagaga tatctctttt gccgcctggg tatagaatga ttccggtaaa
     8461 tcggcgacca gtcgctgcca ggcttgtgcg cggcgttctg gtggcgtcat tactgaatcc
     8521 accccttgca aacggacatt acgcagaata aatggcatga ccgtggttgg cagagtaaaa
     8581 ccacccgcca gaccacaggc cgccacgcag ccgccgtaat tcatttgcgc cagcactttt
     8641 gccagcactt tgtcgccaac agtgtcaatt gccccagccc agacttgttt ttccagagga
     8701 cgggattcgg caaactcatc acgagggaga ataccgctgg cacctaaact tttcagatat
     8761 tcatgggtac tttcgcgacc ggaaacggca acgacctgat aacccaactt atgcagcagc
     8821 gccacggcgg tactgccgac gccaccactg gcacccgtca cgacaatctc cccgtcctgc
     8881 gggcgaacac cggcatcttc cagcgccatc acacacagca tggcggtaaa accggcagta
     8941 ccgataatca ttgctttacg cgcgtccagc ccttgcggca tggcaaccag ccagtcacct
     9001 ttcactcgcg cctgctccgc cagcccaccc cagtggtttt caccaacgcc ccagccagtg
     9061 agtaacacct cctgaccggc atgaaaacgc ggatcttcgc tggtgcgtac agttccggca
     9121 aaatcgatcc caggaatcat cggaaaatta cggatgattt ttcccttacc ggtaatcgcc
     9181 agcgcatctt tatagttcag gctcgaccag tgaacatcga ccgtgacatc gccctccggc
     9241 aggcgacgtt cgtccagagt ctgtactgat gcgagagttt tgccgtcctg ctgttctaaa
     9301 agtaacgcct gcataagtgg tcctcatgtg catgatggat tggaaaataa tttctgaaga
     9361 ctatactcgc taatggaaaa gcaaagccga tgaagcgcaa taaattgcga gataaatctg
     9421 atttgctagt atgcccgctt cctcactatc ggagttaaca caaggatgag attaacgacg
     9481 aaattttcgg cctttgttac gctgctcacc gggttaacaa tttttgtgac tttgctgggc
     9541 tgttcgctaa gtttctacaa cgccattcag tataagttta gtcatcgcgt tcaggcggtg
     9601 gcgacggcga ttgataccca ccttgtgtcg aatgacttca gcgcattaag gccacaaatt
     9661 accgaattaa tggtgtcagc agatatcgtt cgtgtagacc tgctgcatgg tgataaacag
     9721 gtttataccc tggccagaaa tggtagttat cgtccggttg gcaccagcga tctgtttcgt
     9781 gaactgagcg ttccgttgat aaagcatccg gggatgtcgt tgcgtctggt ttatcaggat
     9841 ccgatgggca actatttcca ttcgttgatg accaccgcgc cgctcacggg ggcgattggc
     9901 tttatcattc ttatgctctt tctggcggta cgctggttac aacggcaact tgccgggcaa
     9961 gaattgctgg aaacccgggc tactcgtatc ttaaacggtg agcgtggctc taatgtgttg
    10021 ggaaccatct atgaatggcc gcccagaacc agcagtgcgc tggatacgct gcttcgtgaa
    10081 attcagaacg cacgcgaaca acacagccgt cttgatacgc tgatccgctc ttatgccgca
    10141 caggacgtga aaaccggcct caataaccga ctctttttcg ataatcagtt agcaacgtta
    10201 ctggaagatc aggagaaagt aggtacccac gggatcgtga tgatgattcg tctgccggat
    10261 ttcaatatgt tgagcgatac ctgggggcac agccaggttg aagaacagtt cttcactctg
    10321 acgaatctgc tgtcgacatt tatgatgcgc taccctggcg cactgctggc gcgttaccac
    10381 cgcagtgatt ttgctgcgct gttaccgcac cggacgttaa aagaggcaga gagcatagcc
    10441 agtcagttaa tcaaagccgt cgataccttg ccgaacaata aaatgctcga tcgcgacgat
    10501 atgatccaca ttggtatctg tgcctggcgt agtggtcagg ataccgagca ggtaatggaa
    10561 catgcagagt ctgccacgcg taatgcggga ttgcagggtg gcaatagctg ggctatttat
    10621 gatgactcgt tgcctgaaaa aggacgcggt aatgttcgct ggcgtacgct tatcgagcaa
    10681 atgctcagtc gcggcggccc gcgcctttat caaaaaccgg cggttactcg cgaaggtcag
    10741 gttcatcatc gcgaactcat gtgccgcatc ttcgatggta atgaagaggt tagctcggcg
    10801 gagtatatgc cgatggtctt gcagtttggc ttatcggaag agtatgaccg tctgcaaatc
    10861 agccgtctta ttccactatt gcgttactgg ccagaggaaa atctggcgat tcaggttacc
    10921 gttgagtcgc tgattcgtcc gcgttttcag cgttggctgc gcgatacgtt aatgcaatgt
    10981 gaaaaatcac aacgaaaacg cataattatt gaacttgcag aggccgatgt aggtcaacat
    11041 atcagtcgtt tacaacctgt tattcgttta gtgaatgctt taggggtacg ggtagccgtc
    11101 aaccaggctg gtttgacgct ggtaagcacc agttggatca aagaacttaa tgttgagtta
    11161 ctcaagctcc atccggggct ggtcaggaac attgagaagc gaacggagaa ccagctgctg
    11221 gttcaaagcc tggtggaagc ctgctccggg accagcaccc acgtttacgc caccggcgtg
    11281 cgttcgcgaa gcgagtggca gaccctgatt cagcgcggtg ttacaggcgg gcaaggggat
    11341 tttttcgcgt cctcacagcc acttgatact aacgtgaaaa aatattcaca aagatactcg
    11401 gtttaacctg ccgtttaatc cgttttcacg tagaataacg cgcgctgcgt ctcatgggag
    11461 tgtgcttgtc tgctcgccag attgttgcag cacatatgca gatgaatgac cttacgcggt
    11521 tgcaaacagg cgaggaatgc tgctgatgca ttaagccttt ctggactcag gcagagattt
    11581 gtaacaaagg aaacgaactg cactaatttt caccgtagca gatgattttt gcgccttgtc
    11641 gctgctgcgt gtggttggta aagtaagcgg attttctttt ccgccccagc tttcaggatt
    11701 atcccttagt atgttgaaaa aatttcgtgg catgttttcc aatgacttgt ccattgacct
    11761 gggtactgcg aataccctca tttatgtaaa aggacaaggc atcgtattga atgagccttc
    11821 cgtggtggcc attcgtcagg atcgtgccgg ttcaccgaaa agcgtagctg cagtaggtca
    11881 tgacgcgaag cagatgctgg gccgtacgcc gggcaatatt gctgccattc gcccaatgaa
    11941 agacggcgtt atcgccgact tcttcgtgac tgaaaaaatg ctccagcact tcatcaaaca
    12001 agtgcacagc aacagcttta tgcgtccaag cccgcgcgtt ctggtttgtg taccggttgg
    12061 cgcgacccag gttgaacgcc gcgcaattcg tgaatccgcg cagggcgctg gtgcccgtga
    12121 agtcttcctg attgaagaac cgatggctgc cgcaattggt gctggcctgc cggtttctga
    12181 agcgaccggt tctatggtgg ttgatatcgg tggtggtacc actgaagttg ctgttatctc
    12241 cttgaacggc gtggtttact cctcttccgt gcgcattggt ggtgatcgtt tcgacgaagc
    12301 tatcatcaac tatgtgcgtc gtaattacgg ttctctgatc ggtgaagcca ccgcagaacg
    12361 tatcaagcac gaaatcggtt cggcttaccc gggcgatgaa gtccgtgaaa tcgaagttcg
    12421 tggccgtaac ctggcagaag gtgttccacg cggttttacc ctgaactcca acgaaatcct
    12481 cgaagcactg caggaaccgc tgaccggtat tgtgagcgcg gtaatggttg cactagaaca
    12541 gtgcccgccg gaactggctt ccgacatctc cgagcgcggc atggtgctca ccggtggtgg
    12601 cgcactgctg cgtaaccttg accgtttgtt aatggaagaa accggcattc cagtcgttgt
    12661 tgctgaagac ccgctgacct gtgtggcgcg cggtggcggc aaagcgctgg aaatgatcga
    12721 catgcacggc ggcgacctgt tcagcgaaga gtaatcggat gcaggcaggg gaagcgtctg
    12781 tttaccctgc ctggtctgat acgagaatac gcataactta tgaagccaat ttttagccgt
    12841 ggcccgtcgc tacagattcg ccttattctg gcggtgctgg tggcgctcgg cattattatt
    12901 gccgacagcc gcctggggac gttcagtcaa atccgtactt atatggatac cgccgtcagt
    12961 cctttctact ttgtttccaa tgctcctcgt gaattgctgg atggcatatc gcagacgctg
    13021 gcctcgcgtg accaactaga acttgaaaac cgggcgttac gtcaggaact gttgctgaaa
    13081 aacagtgaac tgctgatgct tggacaatac aaacaggaga acgcgcgtct gcgcgagctg
    13141 ctgggttccc cgctgcgtca ggatgagcag aaaatggtga ctcaggttat ctccacggtt
    13201 aacgatcctt atagcgatca agttgttatc gataaaggta gcgttaatgg cgtttatgaa
    13261 ggccagccgg tcatcagcga caaaggtgtt gttggtcagg tggtggccgt cgctaaactg
    13321 accagtcgcg tgctgctgat ttgtgatgcg acccacgcgc tgccaatcca ggtgctgcgc
    13381 aacgatatcc gcgtaattgc agccggtaac ggttgtacgg atgatttgca gcttgagcat
    13441 ctgccagcga atacggatat tcgtgttggt gatgtgctgg tgacctccgg tctgggcggt
    13501 cgtttcccgg aaggctatcc ggtcgcggtt gtctcttccg taaaactcga tacccagcgc
    13561 gcttatactg tgatccaggc gcgtccgact gcaggactgc aacgtttgcg ttatctgctg
    13621 ctgctgtggg gcgcggatcg taacggcgct aacccgatga cgccggaaga ggtgcatcgt
    13681 gttgctaatg aacgtctgat gcagatgatg ccgcaggtat tgccttcgcc agacgcgatg
    13741 gggccaaagt tacctgaacc ggcaacgggg atcgctcagc cgactccgca gcaaccggcg
    13801 acaggaaatg cagctactgc gcctgttgcg ccgacacagc ccgctgctaa tcgctctcca
    13861 caaagggcta cgccgccgca aagtggtgct caaccgcctg cgcgtgcgcc gggagggcaa
    13921 tagtggcgag ctatcgtagc cagggacgct gggtaatctg gctctctttc ctcattgcgc
    13981 tgttgctgca aatcatgccc tggccggata acctgattgt tttccggcca aactgggtgt
    14041 tacttatctt gttgtattgg atcctggcct tgccccatcg cgtaaatgtg ggcacaggtt
    14101 ttgtgatggg tgccatactg gatctgatca gcggctcgat gcttggcgta cgcgtattgg
    14161 cgatgagcat cattgcttac ctggtggcgc tgaaatacca gcttttccgc aacctcgcat
    14221 tatggcagca ggcgctggtc gtcatgttgc tttcgctggt ggtggatatt attgttttct
    14281 gggcagagtt tttagtgatt aacgtctctt tcagaccgga agtgttctgg agtagtgtag
    14341 tcaatggggt gctctggccg tggattttct tgctgatgcg taaagtccgt cagcagtttg
    14401 cagtgcaata aaggtttcta tgacttctct gtatttagct tccggttctc cgcgtcgtca
    14461 ggagttactt gcgcaacttg gcgtgacctt tgaacgtatt gttacgggca ttgaggagca
    14521 gcgtcagccg caggagagcg cgcagcagta tgttgtgcgt ctggcacgcg agaaagcaca
    14581 ggcgggtgtc gcgcaaacgg cacaggatct cccggtgctg ggtgcggata ttatcgttat
    14641 cctgaacgga gaagtgctcg agaaaccgcg cgacgcagag catgcggcgc agatgttgcg
    14701 caaattatcg ggtcagaccc atcaggtgat gacggcagtg gcgttggccg acagccagca
    14761 cattctcgat tgtctggtgg tcaccgatgt gactttcaga acgttaacag acgaagacat
    14821 cgcgggctat gtcgccagcg gtgaaccgtt agataaagca ggtgcatacg gtattcaggg
    14881 gctgggtggc tgttttgtca ggaagataaa tggcagctat cacgccgtag tcggcttacc
    14941 gctggttgaa acgtatgaat tattaagtaa ttttaacgca ctgcgtgaga aaagggataa
    15001 acatgacggc tgaattgtta gtaaacgtaa cgccttcgga aacgcgagtg gcgtatattg
    15061 atggcggtat tctgcaggaa attcatattg aacgtgaggc gcgacgcgga atagtaggca
    15121 atatctacaa gggtcgtgta agtcgtgtac ttccgggtat gcaggcggct tttgtagata
    15181 ttgggctgga taaagccgcg tttcttcatg catccgacat catgccgcat accgaatgtg
    15241 tggcgggtga agaacaaaag caattcacgg tgcgcgacat ctcggaactg gttcgtcagg
    15301 ggcaagatct gatggtgcag gtggtgaaag atccgcttgg cactaaaggt gcgcgcctga
    15361 ccaccgatat cacgctgcct tctcgctatc tggtatttat gccaggggct tctcacgttg
    15421 gggtttccca acgaattgaa agcgaatcag aacgtgaacg tctgaaaaaa gtggtcgcag
    15481 agtattgcga cgagcagggc gggtttatca tccgcaccgc agcggaaggg gttggcgagg
    15541 ctgaactggc ctccgatgcc gcttatctga aacgcgtctg gaccaaagtt atggagcgta
    15601 aaaagcgccc gcagacccgt tatcagctgt acggcgaact ggcgctggcg cagcgtgttc
    15661 tgcgtgattt tgccgacgcc gaactggacc gcattcgcgt tgactcacgc ctgacttacg
    15721 aagcattgct ggagtttacc tcggagtaca ttcccgagat gaccagcaag ctggagcatt
    15781 acaccggacg ccagccgatt ttcgatctct ttgatgtcga aaacgaaatc cagcgagcgt
    15841 tggaacgcaa agtagaactg aaatccggtg gttatctgat tatcgaccag accgaagcga
    15901 tgaccaccgt ggacatcaat accggagcgt ttgtcggtca tcgcaatctg gacgacacta
    15961 ttttcaatac caatattgaa gcgacgcagg ctatcgctcg ccagttacgg ttgcgtaatc
    16021 tgggcgggat tatcattatt gatttcatcg atatgaataa tgaagatcac cgccgccgcg
    16081 tgctgcattc gctggagcag gcgttgagca aagaccgggt gaaaaccagc gttaatggtt
    16141 tttcggcgct ggggctggtg gagatgacgc gtaaacgcac ccgcgaaagc attgagcacg
    16201 tactgtgtaa cgaatgccca acctgccacg gtcgcggaac ggtgaaaacc gtggaaacgg
    16261 tatgctatga aatcatgcgc gagattgttc gtgtccacca tgcttacgac tccgaccgtt
    16321 tcctggtcta tgcttctccg gcagtagccg aagccttgaa aggcgaagag tcacactcgc
    16381 tggcggaagt ggaaattttc gttggcaaac aggttaaagt acaaattgaa ccgctctata
    16441 accaggagca gtttgacgtc gtaatgatgt aaacagatgc tgggccgcca tccggcaaag
    16501 ggtttttgag tcacattttt agcagacaag gagtgacggg tgaggcgatt gccggggatt
    16561 ttactgctta ctggagccgc gctcgttgtg atcgctgccc tgctggttag cggcctgcgt
    16621 attgctttac cgcatcttaa cgcctggcgt ccggaaatcc tcaacaaaat agaatccgcg
    16681 actggcatgc cggtagaagc cagtcagctc tcagccagct ggcagaattt tggcccgacg
    16741 cttgaagcac acgacatccg tgcagaacta aaagatggcg gcgaattttc ggttaaacgc
    16801 gttactctgg cgctggatgt ctggcagagc ctgttacata tgcgctggca gtttcgcgac
    16861 ctcactttct ggcagctgcg ctttcgcacc aacactccta tcaccagcgg tggtagcgat
    16921 gacagtctgg aagccagtca catcagcgat ctgtttcttc gtcaatttga ccatttcgat
    16981 ctccgcgaca gtgaagtcag ttttctgacg ccatccggtc agcgcgccga gctggcgatc
    17041 ccacaactca cctggctgaa cgatccacgt cgacaccgtg cggaaggcct ggtaagcctc
    17101 tccagcctta ccggacagca cggcgtgatg caggtgcgca tggatttgcg cgatgatgag
    17161 gggttgttaa gcaatggtcg cgtctggcta caggcggatg acatcgacct gaagccgtgg
    17221 ctcggtaaat ggatgcagga caatattgct ctggaaacgg cacagttctc ccttgaaggc
    17281 tggatgacga tcgacaaagg cgatgtaacc ggcggtgacg tctggctgaa acagggcggt
    17341 gccagctggt tgggcgagaa gcaaacgcat acgctgtcgg tggataatct gaccgcgcat
    17401 attacgcgtg aaaatccggg ctggcagttc tctattcccg atacacggat cacgatggac
    17461 ggcaaaccct ggccgagcgg agcattgacg ctggcctgga taccggaaca ggacgttggc
    17521 ggcaaagaca ataaacgcag tgacgaactc cggattcgcg ctagtaatct ggagctggca
    17581 ggcctggagg gcatacgccc gctggccgcg aaactttcac ctgcactggg tgatgtttgg
    17641 cgctccacac aaccgagcgg caagattaac actctggcgc tggatatccc gcttcaggcg
    17701 gcaggcaaga cccgttttca ggcatcgtgg agcgatctgg cctggaagca atggaaatta
    17761 ttaccgggtg cggaacactt ctccgggacg ctttccggca gcgttgaaaa tggtttgctt
    17821 accgcgtcga tgaagcaggc aaagatgcct tacgaaacgg tattccgtgc gccactggaa
    17881 atcgccgacg gccaggcaac tataagctgg ctgaacaatg acaaaggttt ccagctggat
    17941 gggcgtaata ttgacgttaa agccaaagcc gtccatgcgc gcggcggttt tcgttacctg
    18001 caacctgcta acgatgaacc ctggctgggt attctggctg gcatcagtac cgatgatggt
    18061 tcacaagcct ggcgctattt cccggaaaac ttgatgggta aagacttggt tgattattta
    18121 agtggtgcga ttcagggcgg tgaagcggat aacgcgacgc tggtttatgg tggcaatccg
    18181 caactcttcc cctataaaca caacgaaggt cagtttgaag tgctggtgcc gctgcgcaac
    18241 gcgaagtttg ccttccagcc ggactggcct gcattaacta accttgatat tgaactggac
    18301 tttattaacg acggtttatg gatgaaaacc gatggcgtta atctgggcgg cgtgcgcgcg
    18361 agtaatctca ccgcagtgat ccctgactac tcaaaagaaa aactgctgat tgacgctgac
    18421 attaaaggtc cgggtaaagc cgttggccct tactttgatg agacaccgct gaaagattct
    18481 ctgggtgcga ccctgcaaga actccagctc gacggcgatg tgaatgctcg cttacatctt
    18541 gatatcccgc tgaacggcga actggtaacc gcgaaaggtg aagtgacgct gcgtaataac
    18601 agtctgttta tcaaaccact cgacagcatc ctgaaaaatt tgagcggtaa attcagcttt
    18661 atcaatggcg atctgcaaag tgaaccactg acagcaagct ggtttaatca gccgttgaac
    18721 gtggattttt ccaccaaaga aggggcaaaa gcctaccagg ttgcggtgaa tctcaacggt
    18781 aactggcaac cggcgaaaac cggcgttctg cctgaagcgg tgaacgaagc attgagtggc
    18841 agcgtggcgt gggatggtaa agtgggcatt gatctgcctt atcatgctgg cgcgacctat
    18901 aacgtagagc tgaacggcga tctgaataat gtgagtagtc acttaccttc accgttagcc
    18961 aaacctgcgg gtgaaccact agcggtaaac gttaaggttg atggcaatct caacagcttt
    19021 gaattaaccg gacaggctgg tgcggataac catttcaata gccgctggtt gcttggtcaa
    19081 aagctgacgc tcgatcgtgc tatttgggcg gcagacagta aaacgctccc gccgttgccg
    19141 gaacaaagtg gcgttgaact taatatgccg ccgatgaatg gtgccgagtg gctggccctg
    19201 ttccagaaag gcgctgcgga gagtgtcggt ggtgcagcga gtttcccaca acacataacg
    19261 ttacgtacgc ctatgttgtc actgggaaat cagcaatgga ataacctgag tattgtttcg
    19321 caaccgacgg caaatggcac cctggttgag gcgcaagggc gtgaaatcaa cgccacgcta
    19381 gcgatgcgta ataacgcgcc gtggctggcg aatatcaaat atctttatta caacccgagc
    19441 gtggcgaaaa ctcgtggtga ttcaacgccg tcatcacctt tcccgacaac ggagcgcatt
    19501 aacttccgtg gctggccgga cgcacaaata cgatgcacag agtgctggtt ctgggggcaa
    19561 aaattcggtc gcattgacag tgatatcacc atttctggcg atacgttaac gctgaccaat
    19621 ggactgattg atactggttt ctcgcggctt actgccgatg gtgaatgggt taataatccg
    19681 gggaatgaac gtacctcgct gaaaggaaaa ctgcgtgggc agaaaattga tgccgccgca
    19741 gaattttttg gtgtcacgac gcccatacgc cagtcgtcat ttaatgtgga ttacgattta
    19801 cactggcgca aagcaccctg gcagccagat gaagcgacgt tgaatggcat cattcatact
    19861 caactgggta aaggcgaaat taccgaaatc aataccggac atgccgggca attgctgcgc
    19921 ttattgagcg tagatgccct gatgcgtaag ctgcgttttg atttcagaga cacttttggc
    19981 gaagggttct attttgactc cattcgcagc accgcgtgga ttaaagacgg cgttatgcac
    20041 accgacgaca cgctggtgga tggcctggag gcggatatcg ccatgaaagg gtcggtaaat
    20101 ctggtacgcc gcgacctgaa tatggaagcg gttgtcgcac cagagatttc tgcgacggtg
    20161 ggcgtggctg cggcttttgc ggttaacccc attgttggcg cggcagtgtt tgccgccagt
    20221 aaagtgctgg ggccgctgtg gagcaaagtc tccattttgc gctatcacat ttcgggtccg
    20281 ctggacgatc cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa agcgcaatga
    20341 tttgacgagg gcgcgtaatt gccccaatct catatgataa tcgttgccaa aggccaacga
    20401 gccggaacat aactgtaggc cggatagggc gttcacgccg catccggcag ccgtaaaaaa
    20461 tcctctactg cagtaactaa cgagtagcaa aaacgatgag tctgaacctg gtaagtgaac
    20521 aattgctagc ggcgaacggc ctgaaacatc aggacttgtt cgcgatcctc ggtcaactgg
    20581 ccgaacgtcg ccttgattat ggcgatctct attttcagtc gagctatcac gaatcctggg
    20641 ttttagaaga ccgcattatt aaagatggtt cttacaacat cgatcagggc gttggcgtgc
    20701 gtgcaatcag cggtgaaaaa accggatttg cttacgctga ccaaatcagc ctgctggcgc
    20761 tggaacagag tgcgcaagcg gcgcgcacca tcgtccgtga tagtggtgat ggcaaagtac
    20821 agacgctggg cgcggtagag catagcccgt tgtatacctc ggtagatccg ctgcaaagca
    20881 tgagccgtga agagaagctg gatatcctgc gtcgcgtcga taaggttgct cgcgaagcgg
    20941 acaagcgcgt acaggaagtg actgccagcc tcagtggtgt ctatgaatta attctggttg
    21001 cggccaccga cggcacgcta gcggcggatg tccgtccgct ggtgcgtctt tccgtgagcg
    21061 ttctggtcga agaagatggc aaacgcgaac gcggtgccag tggcggcggc ggtcgttttg
    21121 gttatgagtt cttccttgcc gatcttgacg gcgaagtccg tgcggatgca tgggcaaaag
    21181 aagcagtgcg tatggcgctg gtcaatcttt ctgccgttgc tgcaccagcg ggcaccatgc
    21241 cggtagtact tggcgcaggt tggccgggcg tgctgttgca tgaagcggta ggtcacggtc
    21301 tggaaggcga cttcaaccgc cgtggcactt cagtatttag tggacaggtc ggggagctgg
    21361 tggcttcaga actgtgtacc gtggttgatg acggcacgat ggtcgatcgc cgtggttcgg
    21421 tggcgattga tgatgaaggt acgccaggcc agtacaacgt gctgattgag aacggcattc
    21481 tgaaaggcta catgcaggat aaactcaacg cgcgtttgat ggggatgacg ccgactggca
    21541 acggtcgccg tgaatcctac gcccatctgc ccatgccgcg tatgaccaac acctatatgc
    21601 tgccgggtaa atcgaccccg caagaaatta ttgaatccgt tgagtacggt atctatgcac
    21661 cgaactttgg tggcggtcag gtggatatca cctccggcaa attcgttttc tccacttcag
    21721 aagcatatct gattgaaaac ggtaaagtaa cgaagccggt gaaaggcgca acgttgattg
    21781 gttccggtat cgaaaccatg cagcagattt cgatggttgg caacgacctg aaactggata
    21841 acggcgtggg tgtctgcggt aaagaagggc aaagtttgcc ggttggcgtg ggccagccaa
    21901 cgttgaaagt cgataacctg actgttggcg gtactgcgta ataatttcat tattttcaga
    21961 aggataatta atctttctac gtgcacgaac ggtcccctcg ctcctctggg gttagggtga
    22021 ggggaacccg ttggcacagg tttgtagaac gtaacagtac aatatgaatt acttctcttt
    22081 cccgcgcccg tgcatctcct gaaacaattt accgacctca acaaaataat ccgtcagcga
    22141 gttgatcacg acctgtacct tcagcggcag cttatctttt tcggtatata acgcataaac
    22201 cgggcgtgga tctgactgat aacgcggcag caggatctcc agctccccac gattgatctc
    22261 gttgatcacc cacatcagcg gcacgtaggc gatcccggca cccgccgtta gccagcgcac
    22321 cagcgtcatc ggatcattag tcacaaatct cccttgcggg atcaggcgag tcgagatccc
    22381 ttccggtgca atcagttcaa attcattgtc gggccgcacg ctgtattcaa gccatgaatg
    22441 gctacttaaa tcggcgggtt tttccggtat gccgtattgt gtgagatagc ttttcgcggc
    22501 gcacaccacc attggcatcg cgccaagacg gcgggaaaac aggctggaat cctgcaacgc
    22561 gccgacgcgg atcaccacat ccagaccgtc ggcaatcagg tcgggggctg gaattccggt
    22621 aaccagattg gcgctcaaac ctgggtattc tttcagcatt ttggctgtca gcccggcgag
    22681 aacattttgt gccatagttg aagaacagcc aatgcgtagc gtcccgatgg gggtgttatt
    22741 gaaggcatac agttgctcat gaacatcctg cacttcatga agcatacgac ggcagccctg
    22801 gtagtaaatt ctaccggctt cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt
    22861 tacctgcaac tcatcttcca gttttgacac cgtctgactg atggacgaaa cgctcatctg
    22921 tagctgtctg gcggcggcgg taaaagagcc aaattcaact actttggcaa acaccgacat
    22981 gcgttttagt cgttccatta ttcactctga cttaaaagtg atttagatca cataatatag
    23041 ataacagcat aacagttacg ctaatatatt aaatatcaat ctacagcaat gttgctctcg
    23101 cccggctttc catgcccttc tctgcggcga cagatgctga aaataataac gcctgctctc
    23161 tctatacaac caaggtcaac atgagtctgt ttcccgttat cgtggtgttt gggctgtcct
    23221 tcccaccgat attttttgaa ttgcttttat cactggcgat tttctggctg gtgcgccggg
    23281 tacttgtgcc aacaggtatc tacgactttg tctggcatcc ggcgttgttc aacaccgcgc
    23341 tctattgctg cttgttttat ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca
    23401 ctaataagaa aattctcccg tacggccatc acggtcgtat tagtcattct ggccttcatc
    23461 gcaattttta atgcctgggt ctattacacc gaatccccct ggacgcgtga cgcgcgcttt
    23521 agcgctgacg tcgttgcgat cgcgccggac gtttctggac tcattaccca ggtgaatgtt
    23581 catgataacc agctggtgaa aaaaggacag atactgttca ccatcgacca gccgcgctat
    23641 caaaaggcgc ttgaggaagc gcaagccgat gttgcttatt atcaggtact ggcacaggag
    23701 aaacgccagg aggccggacg tcgtaaccgt ctcggtgtgc aggcgatgtc tcgcgaagag
    23761 atcgaccagg ctaacaacgt actacaaacg gttctgcatc agttagcgaa agcgcaggcg
    23821 acccgcgatc tggcaaaact ggatcttgaa cgcacggtga tccgagcgcc agcagatggc
    23881 tgggtgacca acctcaacgt ctataccggt gagtttatta ctcgaggatc aacggcggtt
    23941 gcgctggtga aacagaactc cttctatgta ctggcctata tggaagaaac taagctggaa
    24001 ggggtgcgtc cggggtatcg tgcagagatc acgccgcttg gcagtaacaa agtgctgaaa
    24061 gggactgttg atagtgttgc cgctggggtc accaacgcca gcagcacgcg tgacgacaaa
    24121 gggatggcga ccatcgactc tgaccttgaa tgggtgcgtt tggcgcaacg tgttccggtt
    24181 cgtattcgtc tcgacaacca gcaagagaac atctggcctg cgggcaccac tgctacagtg
    24241 gtggtcactg gcaaacaaga tcgcgacgaa agccaggatt cgttcttccg taaaatggcc
    24301 catcgcctgc gtgagtttgg ttaatcacga tgggtatttt ctccattgct aaccaacata
    24361 ttcgctttgc ggtaaaactg gcgaccgcca ttgtactggc gctgtttgtt ggctttcact
    24421 tccagctgga aacgccacgc tgggcggtac tgacagcggc gattgttgcc gccggtccgg
    24481 cctttgctgc gggaggtgaa ccgtattctg gcgctattcg ctatcgtggc tttttgcgca
    24541 tcatcggcac atttattggc tgtattgccg gactggtgat catcattgcg atgatccgcg
    24601 caccattatt gatgattctg gtgtgctgta tctgggccgg tttttgtacc tggatatcct
    24661 cgctggtacg aatagaaaac tcgtacgcgt gggggctggc cggttatacc gcgctgatca
    24721 ttgtgatcac cattcagccg gaaccattgc ttacgccgca gtttgccgtc gaacgttgta
    24781 gcgagatcgt tatcggtatt gtgtgtgcga ttatggcgga tttgctcttt tctccgcgat
    24841 cgatcaaaca agaagtagat cgagagctgg aaagtttgct ggtcgcgcaa tatcaattaa
    24901 tgcaactctg tatcaagcat ggcgatggtg aagttgtcga taaagcctgg ggcgacctgg
    24961 tgcgacgcac cacggcacta caaggcatgc gcagcaacct gaatatggaa tcttcccgct
    25021 gggcgcgggc caatcgacgt ttaaaagcga tcaatacgct atcgctgacg ctgattaccc
    25081 aatcctgcga aacttatctt attcagaata cgcgcccgga attgatcact gatactttcc
    25141 gcgaattttt tgacacgccg gtagaaaccg cgcaggacgt ccacaagcag ctcaaacgcc
    25201 tgcggagagt tatcgcctgg accggggaac gggaaacgcc tgtcaccatt tatagctggg
    25261 tcgcggcggc aacgcgttat cagcttctca agcgcggcgt tatcagtaac acaaaaatca
    25321 acgccaccga agaagagatc ctgcaaggcg aaccggaagt aaaagtagag tcagccgaac
    25381 gtcatcatgc aatggttaac ttctggcgaa ccacactttc ctgcattctg ggcacgcttt
    25441 tctggctgtg gacgggctgg acttccggca gtggtgcaat ggtgatgatt gcggtcgtga
    25501 cgtcactggc aatgcgtttg ccgaatccac gcatggtggc gatcgacttt atctatggga
    25561 cgctggccgc gctgccgtta gggctgctct actttttggt gattatccct aatacccaac
    25621 agagcatgtt gctgctgtgt attagcctgg cagtgctggg attcttcctc ggtatagaag
    25681 tacagaaacg gcgactgggc tcgatggggg cactggccag caccataaat attatcgtgc
    25741 tggataaccc gatgactttc catttcagtc agtttctcga cagcgcatta gggcaaatcg
    25801 tcggctgtgt gctcgcgttc accgttattt tgctggtgcg ggataaatcg cgcgacagga
    25861 ctggacgtgt actgcttaat cagtttgttt ctgccgctgt ttccgcgatg actaccaatg
    25921 tggcacgtcg taaagagaac cacctcccgg cactttatca gcagctgttt ttgctgatga
    25981 ataagttccc aggggatttg ccgaaatttc gcctggcgct gacgatgatt atcgcgcacc
    26041 agcgcctgcg tgatgcgccg atcccggtta acgaggattt atcggcgttt caccgacaaa
    26101 tgcgccgcac agcagaccat gtgatatctg cccgtagcga tgataaacgt cgtcggtact
    26161 ttggccagtt gctggaagaa ctggaaatct accaggaaaa gctacgcatc tggcaagcgc
    26221 caccgcaggt gacggaaccg gttcatcggc tggcggggat gctccataag tatcaacatg
    26281 cgttgaccga tagttaagtc aaaaccgacg ccaaaagcgt cggttttttc atggctatac
    26341 ttagcgatgc acggcagaac tcgccgcgaa acgtgacggt ggcaacagat gaatatttat
    26401 gcctttgatt ttgatgagat tgagagtcag gaggattttt atcgtgactt tagccaaacc
    26461 tttggtctgg ctaaagataa ggtacgcgat ctcgactcac tatgggatgt gttaatgaac
    26521 gatgtcctgc cgctaccact tgagattgaa tttgttcatc tgggagagaa aacgcgtcgc
    26581 cgttttggcg cgttaatatt gctgtttgat gaggcagagg aagagctgga agggcatttg
    26641 cgttttaatg ttcgtcatta gcgcaaaaaa aagcccacga accgggggca atatcgtcgg
    26701 acaaaacgat gagggtttat ttgtacagtt cagccgtagc gtgccaggtg tcaccgctac
    26761 gagcttcagt aatctggtag gccgttgcgc ctttctcttc cgcttttttg ttcagcattt
    26821 cacgtatatc cattggcgaa gacgccacac cacttacgga tacggtcccg attgcttcac
    26881 gattttgtgc ttgtgcagca tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag
    26941 aaagtacgct taatgcagct acagtggttt tgattttcat gatttttacc tcgacataat
    27001 cttttagctg ggcctttgtt tcgtgaccca tatcacaaaa tcaagtatac actaattatt
    27061 gaactaatta ataccacact aactgtttca gttgatacta agtgttaaaa atgtgaattt
    27121 taataacaaa aaagtataaa aaacacggtt atttattatt aattatagta ttttcaatta
    27181 gataatgaat tttgcataat gtgctttttt taccgtatgt attcattttg tgaatgatgt
    27241 gtcgcggtaa aacgcactat tcgttaaatg tatcgcagga gaaagaaggg gaatgagagg
    27301 gataagcaac attttccccg ccgccagaag cgacggggca gagattaaag ctcctggtcg
    27361 aacagctcta aaatcgcttc gtacaggtct ttgacggtga aaccgttagc aggggtggta
    27421 aagatggtgt catcgccagc gatggtgccc agaatacctt ctgctttgcc cagtgagtcc
    27481 agcaggcgag caattaactg cgccgcgcca gggctggtat gaatcacgac aactgcatcg
    27541 ttgtagtcga tatccagcac cagattcttc aatggactgg aggtggttgg tacacccagt
    27601 tcagctggca ggcagtaaac catttccatt ttggcattgc gtgtacgtac agcaccaaac
    27661 ttggtcaaca tccgcgagac tttagactga ttaatattgt caaagccttg ctcctgcaac
    27721 gcggcgacga tttcgccctg ggagctaaat ttctcttctt taagtaatgc tttaaatgct
    27781 ttaactagtt cttcttgctt agccgagctt cgcataagtc acccgatatg gtggttgata
    27841 caacattatt gtgcatacag atgaattttt atgcaaacag tcagccctga agaaggctga
    27901 aataatgtta tgaaagagcg ggattttatc aaatttcgtt attgagaaac atgcctgcgt
    27961 cacggcatgc aaattctgtt taaaagtaaa ttaattgtta tcaaattgat gttgttttgg
    28021 ctgaacggta gggtatattg tcaccacctg ttggaatgtt gcgctaatgc ataagcgact
    28081 gttaattacg taagttaggt tcctgattac ggcaattaaa tgcataaacg ctaaacttgc
    28141 gtgactacac attcttgaga tgtggtcatt gtaaacggca attttgtgga ttaaggtcgc
    28201 ggcagcggag caacatatct tagtttatca atataataag gagtttagga tgaaagtcgc
    28261 agtcctcggc gctgctggcg gtattggcca ggcgcttgca ctactgttaa aaacccaact
    28321 gccttcaggt tcagaactct ctctgtatga tatcgctcca gtgactcccg gtgtggccgt
    28381 cgatctgagc catatcccta ctgctgtgaa aatcaaaggt ttttctggtg aagatgcgac
    28441 tccggcgctg gaaggcgcag atgtcgttct tatctctgca ggcgtagcgc gtaaaccggg
    28501 tatggatcgt tccgacctgt ttaacgttaa cgccggcatc gtgaaaaacc tggtacagca
    28561 agttgcgaaa aactgcccga aagcgtgcat tggtattatc actaacccgg ttaacaccac
    28621 agttgcaatt gctgctgaag tgctgaaaaa agccggtgtt tatgacaaaa acaaactgtt
    28681 cggcgttacc acgctggata tcattcgttc caacaccttt gttgcggaac tgaaaggcaa
    28741 acagccaggc gaagttgaag tgccggttat tggcggtcac tctggtgtta ccattctgcc
    28801 gctgctgtca caggttcctg gcgttagttt taccgagcag gaagtggctg atctgaccaa
    28861 acgtatccag aacgcgggta ctgaggtggt tgaagcgaaa gccggtggcg ggtctgcaac
    28921 cctgtctatg ggccaggcag ctgcacgttt tggtctgtct ctggttcgtg cactgcaggg
    28981 cgaacaaggc gttgtcgaat gtgcctacgt tgaaggcgac ggtcagtacg cccgtttctt
    29041 ctctcaaccg ctgctgctgg gtaaaaacgg cgtggaagag cgtaaatcta tcggtaccct
    29101 gagcgcattt gaacagaacg cgctggaagg tatgctggat acgctcaaga aagatatcgc
    29161 cctaggcaaa gagttcgtta ataagtaatt gattagcgaa taataaaaaa ccggagcaca
    29221 gactccggtt tttgttttga gcactcgact taattggttg ccggatattc ctgaatggtg
    29281 acctgcagcg ttaactgctt atcatcacgc atcactacaa ccgggatcac cgaaccaggg
    29341 cgaatttccg ccacctgatc catcgtctcc agagcagaga tggccggttt gttatccacc
    29401 gaaataatca gatcgttgac ctgaataccc gcattcgccg ctgggccgtc aggtgacact
    29461 tcattaacca cgatcccttg cagttgatct ataccaccgc tctgcgcgtg cagtggtgcg
    29521 atctcgcgtc cgccgatacc aatgtagccg cggatcacgc gaccatcgcg gatcagctta
    29581 tccataattt tggttgctaa ctggaaagga atcgcaaagc cgataccttc cggcgtttcg
    29641 ccatcgttac tcttatcaaa cgacagcgtg ttaatgccca tcagttctcc cagcgagttc
    29701 accagcgcgc cgccagagtt accgtggtta atggaagcat cggtttgtag gaagttttgc
    29761 cgcccggtcg ggttcagacc gattcgaccc gtggcactaa taatcccttg ggtaatggtc
    29821 tgcccgaggt tgtacgggtt accgatcgcc agtactacgt cgccaatgtg cggtacgcga
    29881 cgtgcattaa ttggaatggt aggtaaaccg ccagtggcat taattttaag taccgccaga
    29941 tcggttagag agtcagatcc caccagcaat gcttcaaata cacgtccatc ctgtaaggcg
    30001 acgatgatct gatcggcgtc gttgatgacg tgtttattgg tgatgatata accgcgttga
    30061 tccatgatta caccggatcc cagggtgcgg atctcaagct ggttgtgaga gttggtgttc
    30121 aaaccacggt tgtaaacgtt aaccaccgct ggcgcggcgc ggcgaaccgc cagattatag
    30181 ctggcaggcg tctcatcggt actgtcaaat tgcggagtgg aaagcgggtt aaggctgcgc
    30241 agcgaaggca tggcaaccag cagaatagcg ccgacaatta atccaatcgc aacggaacgt
    30301 aagagcttca caaacatgat ggaggcgtca ttaaaaaagg gaacggcagc agcataccac
    30361 gagttaaccg gacatcacac gtcagcctga tgcccggttt acgacattaa cgcatcagca
    30421 gatagatgct ttcattgccg cgtacaattt gcagggcgat gatggccggt tttgccgcca
    30481 gcactttacg catttcagca atcgagttca cccgatcgcg gttgacgcca atgatcacat
    30541 cgtctttttg caagccagcc tgagcagctg ggcttccttt gacaacttca tctatcttaa
    30601 tacctttgcc gccatctttt agctgaccat cgctcaacgt tgcaccttcc agcgctggcc
    30661 gacgaagagg tgctggtatc aagcgtcact tctacttcca gtggtttgcc gttacgcagc
    30721 aggccaagct tcactttcgt gcccggctct gtagtcgcga tacgagagcg caactcagca
    30781 aagctattca gcggtttgcc gttgaggctg gtaataatat cgcccgcttt gacgcccgct
    30841 ttcgctgagc cagaacttgg caacacttcg ctgacaaacg cgccacgctg cacgtcaagg
    30901 ttgaaggctt tggcgatatc ggcactcatc tcggtgcctt tgatgcctaa caaaccgcgt
    30961 ttgatttcac caaagtcgat aagctgctgc gccagtgttc gcgccatatt actggggatg
    31021 gcaaatccaa tcccgacgct cccgccgcca ggcgcaagga ttgcagtgtt gatgccaatt
    31081 aactcaccgt taaggtttaa tagtgcaccg ccggagttac cgcggttaat ggaagcatct
    31141 gtctggataa agttttccag accttcaaga ttcaacccgc tgcggcctaa tgcggaaaca
    31201 atgccagagg tggcggtttg cccaaggcca aatgggttac cgaccgctac ggcaaaatca
    31261 ccgacgcgca atttatcgga gtcggcaata gcgatttgcg ttaatttgct cgggttttga
    31321 atttgtaaca gggcgatatc gctctggtca tcgctaccaa tcagttttgc atcaaactcg
    31381 cgcccatcat tgagctgaat actgattttc tgtgcctgat taatcacatg gttgttggtc
    31441 agcacatagc ctttactggc gttgatgatg acaccggaac ctaaaccttc gaagggttgt
    31501 gcaggttgat ccggtaaatc atcaccaaaa aactttttga attcttccgg gattttctgt
    31561 ccctgactgg ccgttccttc cacccgtacg ctcaccactg ccggaagcac tttttccagc
    31621 attggagcca gactggggag aggggcctga tcggcaacct ggcctggaat cgacgcgacg
    31681 gcctgaaatg acgccgagag agttaacccg gcacttaacg ctaatgcact caacagctgg
    31741 gtttgttttt tcattattcc tgctctcgta cctgaatgat aagaaaagag attcaaaaca
    31801 gtttgatgtt attgaatttt cagacggcta acaatgagac accagattaa atgatagtcg
    31861 gggagggaga gaagaggagg gcgcaatggc tgcgcccgaa aaataaatta gtcgcgcttc
    31921 gcgccagtac gcagcaggcc ggatgcgcct tcagaatagt cgcgaggcac ctgcaccggt
    31981 gcctgatcgt tgctggcttc agactctgcc agacgattac ggaacgggtt tgcttcagca
    32041 gacagttccg gcagcaggct gctggagctt tttgccatgt gctgatacag ctggcgatag
    32101 tcgtgcgcca tggtatccag taattccgcg ctgcgggcaa agtggctaac cagctcttcg
    32161 cgatactcgt ccagttcagc tttattcttt tccagttcgt actgcaacgc ctgttgctgg
    32221 cgtagtttac gattaccaaa acgcatggcc acagcaccaa taatgatgcc gacgactaac
    32281 ccaattagcg catattccca ggtcatgaac atctcccgtt gtcttttgtt tccgtagggt
    32341 gttggcttca ggctccatgc ctgcggctga ttatgccact ataaccgtta attccacaga
    32401 agtggaatcc cgactgcata tcgcgtagtg tagaacggcc tttttttcgt caaccgtgaa
    32461 caacagcgca ccgattattc aaggaataac aataagatca tgcaaagcgt taccccaaca
    32521 tcgcaatacc tgaaggcgct caatgaaggc agccatcaac ccgacgacgt tcaaaaagag
    32581 gccgtcagcc gcctggaaat tatttatcaa gaactcatca atagcaggcc accagccccc
    32641 aggacgagtg ggctaatggc gcgggtcggt aagctgtggg gtaaacgcga agacacaaag
    32701 catacgccag tgcgtggctt atatatgtgg ggcggtgtag gacgcgggaa aacctggctg
    32761 atggaccttt tctatcaaag cctgccggga gagcggaaac agcgcctgca ctttcaccgt
    32821 tttatgctgc gggtgcacga agagctaact gccttacagg ggcagaccga tccgctggaa
    32881 attattgccg atcgctttaa agccgaaact gacgtgctct gttttgacga attttttgtt
    32941 tctgatatta ccgacgccat gctacttggc ggtctgatga aagccctgtt cacccgcggt
    33001 attaccctgg tagcgacgtc aaatattccg ccggacgaac tttatcgaaa tggcctgcaa
    33061 cgtgcgcgtt ttctgcctgc aatcgatgcc attaaacagc attgtgatgt aatgaacgtg
    33121 gacgctggtg ttgattatcg actgcgtaca ctcactcagg cgcatctgtg gctttcgcca
    33181 ctcaacgatg aaacccgggc gcagatggat aaactatggt tggcgctggc gggggtgaaa
    33241 cgagaaaatt caccgacgtt agaaatcaac catcggccat tggcgacaat gggcgtcgag
    33301 aaccagacgc tggcggtttc ttttactacg ctgtgcgtcg acgcccgcag tcagcatgac
    33361 tatattgcgc tctcacgcct gtttcacaca gtaatgttgt ttgatgtgcc agtcatgacg
    33421 cggttgatgg agagcgaagc gcggcgcttt attgcgctgg tggatgagtt ttacgagcgc
    33481 catgtcaaat tagtggtgag tgcagaagtg ccgctgtatg aaatttatca gggtgagcgg
    33541 ctgaagtttg agttccagcg ttgcctgtca cgtctgcaag agatgcaaag cgaagagtat
    33601 ctgaagcgcg agcatttggc gggttaaaac ctgccacaaa tcacaaaaag gggtcgatct
    33661 ttgaccccga cttctctata atcctgcgac cccacgttac aagaaagttt ttttccccaa
    33721 aactttttgt gtgctggcat aggctattcg aaggggtagg tttgccggac tttgtcgtgt
    33781 gaacctcaac aattgaagac gtttgggtgt tcaccaacgt gtaactattt attgggtaag
    33841 cttttaatga aaacttttac agctaaacca gaaaccgtaa aacgcgactg gtatgttgtt
    33901 gacgcgaccg gtaaaactct gggccgtctg gctactgaac tggctcgtcg cctgcgcggt
    33961 aagcacaaag cggaatatac tccgcacgta gataccggtg attacatcat cgttctgaac
    34021 gctgacaaag ttgctgtaac cggcaacaag cgtactgaca aagtgtacta tcaccacacc
    34081 ggccacatcg gtggtatcaa acaagcgacc tttgaagaga tgattgctcg ccgtcctgag
    34141 cgtgtgattg aaatcgcggt taaaggcatg ttgccaaaag gcccgctggg tcgtgctatg
    34201 ttccgtaaac tgaaagttta cgcgggtaac gagcacaacc acgcggcaca gcaaccgcaa
    34261 gttcttgaca tctaatcggg attataggca atggctgaaa atcaatacta cggcactggt
    34321 cgccgcaaaa gttccgcagc tcgcgttttc atcaaaccgg gcaacggtaa aatcgtaatc
    34381 aaccaacgtt ctctggaaca gtacttcggt cgtgaaactg cccgcatggt agttcgtcag
    34441 ccgctggaac tggtcgacat ggttgagaaa ctggacctgt acatcaccgt taaaggtggt
    34501 ggtatctctg gtcaggctgg tgcgatccgt cacggtatca cccgcgctct gatggaatac
    34561 gacgagtccc tgcgttctga actgcgtaaa gctggcttcg ttactcgtga cgctcgtcag
    34621 gttgaacgta agaaagtcgg tctgcgtaaa gcacgtcgtc gtccgcagtt ctccaaacgt
    34681 taattggctt ctgctacggc agaaaacaat ttgcgaaaaa acccgcttcg gcgggttttt
    34741 ttatggctaa attctgaatc agcgtaaaaa atgggaagtt gttttttgct gccgtctgaa
    34801 agacaggtaa aacaaaaacc acatcgccaa taagggtcta agtcaactat ttcagactaa
    34861 agcgcatctc tttttcccca tttccggcat ccactcacca caaaggtcgc aaaatctggt
    34921 aaactatcat ccaattttct gcccaaatgt cgggtattgc tcattttttg tttgattttc
    34981 gaacaaagag agtcgttcct tattgggtaa cacaacttct gactggccac ctggtggctg
    35041 gtagcagtaa aaattctgac tatacctgga ggttttcatg gctgtcgctg ccaacaaacg
    35101 ttcggtaatg acgctgtttt ccggtcctac tgacatctat agccatcagg tccgcattgt
    35161 gctggctgag aaaggtgtaa gtttcgagat cgaacacgtg gaaaaggaca atccgcctca
    35221 ggatctgatt gacctcaacc cgaatcagag cgttccgacc ctggtggatc gtgagctgac
    35281 cctgtgggaa tctcgcatca ttatggaata tctggatgag cgtttcccgc atccgccact
    35341 gatgcctgtt tacccggtag ctcgcggtga aagccgtctg tacatgcatc gcatcgaaaa
    35401 agactggtac acgctgatga acactatcat caacggttca gcttctgaag cagatgccgc
    35461 acgtaagcaa ctgcgcgaag aactgctggc gattgcgccg gtcttcggtc agaagccgta
    35521 cttcctgagc gatgagttca gcctggtcga ttgctacctt gctccgctgc tgtggcgtct
    35581 gccgcaactg ggcatcgagt tcagcggccc gggtgcgaaa gagctgaaag gctatatgac
    35641 ccgcgtcttt gagcgtgact ctttccttgc ttctttaact gaagcagaac gtgaaatgcg
    35701 tctgggccgg agttaatctg tatggatttg tcacagctaa caccacgtcg tccctatctg
    35761 ctgcgtgcat tctatgagtg gttgctggat aaccagctca cgccgcacct ggtggtggat
    35821 gtgacgctcc ctggcgtgca ggttcctatg gaatatgcgc gtgacgggca aatcgtactc
    35881 aacattgcgc cgcgtgctgt cggcaatctg gaactggcga atgatgaggt gtgctttaac
    35941 gcgcgctttg gtggcattcc gcgtcaggtt tctgtgccgc tggctgccgt gctggctatc
    36001 tacgcccgtg aaaatggcgc aggcacgatg tttgagcctg aagctgccta cgatgaagat
    36061 accagcatca tgaatgatga agaggcatcg gcagacaacg aaaccgttat gtcggttatt
    36121 gatggcgaca agccagatca cgatgatgac actcatcctg acgatgaacc tccgcagcca
    36181 ccacgcggtg gtcgaccggc attacgcgtt gtgaagtaat acaaaacagg cccaggcggc
    36241 ctgttttgtc tttttaatga cttaataaaa tagcgtgatt gtggcaatca tattcaccac
    36301 gaaaccgact gccatgggga tcgctgtccg cttcaccacc gcaaaaggcg aaacgcctgc
    36361 aattcctgac gtaacgacca ccaccgcagt aatcggcgaa accgcgcgcg ctagcgtcgt
    36421 ggcaaaatgc atcggcataa tcattacaac cgctggtaca tgtagtccgg ctgcgatatt
    36481 cggaataaga ctggcaaatg acataaacgg cgcattgcca gagcccatca caatggcaca
    36541 aatggcaatg actagcgcca taataatcat cacgccaata ccgcccagac cagaatgctc
    36601 cgcacccctg ataaccgcat caacagtgcc aatcgtcgtt aagcctttcg caaatatttc
    36661 acccgcgacc accagcgtta ccacgttgac aaactgcgta cccatgccgt caaaaaacgc
    36721 ctgcacatcg tccattgttt cgcgcaagtt atgcttgcgg aagaactcaa caaacatcgt
    36781 caccgtcaaa cttagtaaca tcaccaccac cagatgcagt tctgacttca tcagcccgac
    36841 gtgggcgagg aacagcgagc cgagcatcag gattaacggc atcacaggta aaatggcgta
    36901 atagagcggc gggacattat cgagagtttt ttgctctgcc tgttcgtgat tgatattttt
    36961 atcttttttg tcaaaagcgc gttgcacgaa aaagtgggag atcgccaccg agataatgac
    37021 gcaagaggcg accggaagct ggtagtggaa gaagtatgtg gcaattttca ttcccgcgac
    37081 ctgggctgca aaaatggagt tcgtttccag aatcccccac tcaatggaca tcgtggttgc
    37141 gataaccgcc accgcagaga ggcgacttac tcccagactc accagcgtcg gaaataaggt
    37201 gaccatcagc aacataccca gaccggaggc gctggtaata aactgcgcca tgatttggcc
    37261 gatgacgtaa gttgccgaca gaataatata cggcgagcga atgagtttta acgggcggct
    37321 tagcaagctc accatcgcgc aactggctcc tatgcgctcc atgtagcggg cataaccgcc
    37381 caccgccata atcgacagcc ccaatccggc aatgcggttg gacagcatgc gcaggatttc
    37441 attataaatg tcgacgatca agtagccgct gcttttggcg ggatcgagta caccaccgaa
    37501 ccctaaccag actccgcaca tcatcaggaa gacacctgcg gcagccagca ccacctgagg
    37561 tttgtagttt ttcaggatca aatagcccat cgtaattaat acgatgacag atataattat
    37621 gccgaacatt gttgggtaat gactccaact tactgatagt gttttatgtt cagataatgc
    37681 ccgatgacct tgtcatgcag ctccaccgat tttgagaacg acagtgactt ccgtcccagc
    37741 cttgccagat gttgtctcag attcagatta tgtcgctcaa tgcgctgagt
//
LOCUS       DAEEDS010000033        36835 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEEDS010000033 DAEEDS010000000
VERSION     DAEEDS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36835
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.033"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..247
                     /locus_tag="KLH32_001846"
     CDS             <1..247
                     /locus_tag="KLH32_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBE0138696.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KLH32_001847"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KLH32_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBE0138697.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KLH32_001848"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KLH32_001848"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE0138698.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="KLH32_001849"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="KLH32_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBE0138699.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="KLH32_001850"
     CDS             complement(3258..3542)
                     /locus_tag="KLH32_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138700.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KLH32_001851"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KLH32_001851"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBE0138701.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="KLH32_001852"
     CDS             complement(4443..5651)
                     /locus_tag="KLH32_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0138702.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="KLH32_001853"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="KLH32_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBE0138703.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="KLH32_001854"
     CDS             complement(6446..8131)
                     /locus_tag="KLH32_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBE0138704.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="KLH32_001855"
     CDS             8401..8778
                     /locus_tag="KLH32_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBE0138705.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KLH32_001856"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KLH32_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBE0138706.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="KLH32_001857"
     CDS             9225..9512
                     /locus_tag="KLH32_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBE0138707.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="KLH32_001858"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="KLH32_001858"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBE0138708.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="KLH32_001859"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="KLH32_001859"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBE0138709.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="KLH32_001860"
     CDS             11269..11745
                     /locus_tag="KLH32_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBE0138710.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="KLH32_001861"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="KLH32_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBE0138711.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="KLH32_001862"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="KLH32_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBE0138712.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="KLH32_001863"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="KLH32_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBE0138713.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="KLH32_001864"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="KLH32_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBE0138714.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="KLH32_001865"
     CDS             16302..16790
                     /locus_tag="KLH32_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBE0138715.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="KLH32_001866"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="KLH32_001866"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBE0138716.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KLH32_001867"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KLH32_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBE0138717.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KLH32_001868"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KLH32_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBE0138718.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KLH32_001869"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KLH32_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBE0138719.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KLH32_001870"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KLH32_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0138720.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KLH32_001871"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KLH32_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBE0138721.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="KLH32_001872"
     CDS             complement(22242..22757)
                     /locus_tag="KLH32_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0138722.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="KLH32_001873"
     CDS             23406..25175
                     /locus_tag="KLH32_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE0138723.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="KLH32_001874"
     CDS             25386..25709
                     /locus_tag="KLH32_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBE0138724.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="KLH32_001875"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="KLH32_001875"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBE0138725.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTSVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="KLH32_001876"
     CDS             complement(26533..27546)
                     /locus_tag="KLH32_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE0138726.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="KLH32_001877"
     CDS             complement(27645..29075)
                     /locus_tag="KLH32_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBE0138727.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KLH32_001878"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KLH32_001878"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBE0138728.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KLH32_001879"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KLH32_001879"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBE0138729.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KLH32_001880"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KLH32_001880"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBE0138730.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KLH32_001881"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KLH32_001881"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBE0138731.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KLH32_001882"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KLH32_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBE0138732.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KLH32_001883"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KLH32_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBE0138733.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9215 a         9156 c         9442 g         9022 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacatc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaa
//
LOCUS       DAEEDS010000034        36520 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEEDS010000034 DAEEDS010000000
VERSION     DAEEDS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.034"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(156..1391)
                     /locus_tag="KLH32_001884"
     CDS             complement(156..1391)
                     /locus_tag="KLH32_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE0138734.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="KLH32_001885"
     tRNA            complement(1538..1614)
                     /locus_tag="KLH32_001885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="KLH32_001886"
     tRNA            complement(1657..1743)
                     /locus_tag="KLH32_001886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="KLH32_001887"
     tRNA            complement(1764..1839)
                     /locus_tag="KLH32_001887"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="KLH32_001888"
     tRNA            complement(1898..1974)
                     /locus_tag="KLH32_001888"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="KLH32_001889"
     CDS             complement(2077..3462)
                     /locus_tag="KLH32_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0138735.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KLH32_001890"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KLH32_001890"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBE0138736.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KLH32_001891"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KLH32_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBE0138737.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KLH32_001892"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KLH32_001892"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBE0138738.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KLH32_001893"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KLH32_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBE0138739.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KLH32_001894"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KLH32_001894"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBE0138740.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KLH32_001895"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KLH32_001895"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBE0138741.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KLH32_001896"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KLH32_001896"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE0138742.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KLH32_001897"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KLH32_001897"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE0138743.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KLH32_001898"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KLH32_001898"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBE0138744.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KLH32_001899"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KLH32_001899"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBE0138745.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KLH32_001900"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KLH32_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBE0138746.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KLH32_001901"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KLH32_001901"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBE0138747.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KLH32_001902"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KLH32_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBE0138748.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KLH32_001903"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KLH32_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBE0138749.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KLH32_001904"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KLH32_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBE0138750.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="KLH32_001905"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="KLH32_001905"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBE0138751.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="KLH32_001906"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="KLH32_001906"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBE0138752.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KLH32_001907"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KLH32_001907"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBE0138753.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="KLH32_001908"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="KLH32_001908"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBE0138754.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KLH32_001909"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KLH32_001909"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBE0138755.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="KLH32_001910"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="KLH32_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBE0138756.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KLH32_001911"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KLH32_001911"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBE0138757.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KLH32_001912"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KLH32_001912"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBE0138758.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KLH32_001913"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KLH32_001913"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBE0138759.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KLH32_001914"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KLH32_001914"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBE0138760.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="KLH32_001915"
     CDS             complement(31267..32913)
                     /locus_tag="KLH32_001915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBE0138761.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="KLH32_001916"
     CDS             complement(32916..32966)
                     /locus_tag="KLH32_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138762.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KLH32_001917"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KLH32_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBE0138763.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="KLH32_001918"
     CDS             33502..35022
                     /locus_tag="KLH32_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBE0138764.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KLH32_001919"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KLH32_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBE0138765.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="KLH32_001920"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="KLH32_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBE0138766.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="KLH32_001921"
     tRNA            complement(36439..36514)
                     /locus_tag="KLH32_001921"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8918 a         9996 c         9200 g         8406 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg tactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcatat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgtacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acattaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAEEDS010000035        35155 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEEDS010000035 DAEEDS010000000
VERSION     DAEEDS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35155
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.035"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            88..1116
                     /gene="murB"
                     /locus_tag="KLH32_001922"
     CDS             88..1116
                     /gene="murB"
                     /locus_tag="KLH32_001922"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBE0138767.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1113..2078
                     /gene="birA"
                     /locus_tag="KLH32_001923"
     CDS             1113..2078
                     /gene="birA"
                     /locus_tag="KLH32_001923"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBE0138768.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KLH32_001924"
                     /gene_synonym="panK"
     CDS             complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KLH32_001924"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBE0138769.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            3419..3494
                     /locus_tag="KLH32_001925"
     tRNA            3419..3494
                     /locus_tag="KLH32_001925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3452..3454,aa:Thr,seq:tgt)
     gene            3503..3587
                     /locus_tag="KLH32_001926"
     tRNA            3503..3587
                     /locus_tag="KLH32_001926"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Tyr,seq:gta)
     gene            3704..3778
                     /locus_tag="KLH32_001927"
     tRNA            3704..3778
                     /locus_tag="KLH32_001927"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3737..3739,aa:Gly,seq:tcc)
     gene            3785..3860
                     /locus_tag="KLH32_001928"
     tRNA            3785..3860
                     /locus_tag="KLH32_001928"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Thr,seq:ggt)
     gene            3975..5159
                     /gene="tuf"
                     /locus_tag="KLH32_001929"
     CDS             3975..5159
                     /gene="tuf"
                     /locus_tag="KLH32_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0138770.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5389..5772
                     /gene="secE"
                     /locus_tag="KLH32_001930"
     CDS             5389..5772
                     /gene="secE"
                     /locus_tag="KLH32_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBE0138771.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            5774..6319
                     /gene="nusG"
                     /locus_tag="KLH32_001931"
     CDS             5774..6319
                     /gene="nusG"
                     /locus_tag="KLH32_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBE0138772.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6478..6906
                     /gene="rplK"
                     /locus_tag="KLH32_001932"
     CDS             6478..6906
                     /gene="rplK"
                     /locus_tag="KLH32_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBE0138773.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            6910..7614
                     /gene="rplA"
                     /locus_tag="KLH32_001933"
     CDS             6910..7614
                     /gene="rplA"
                     /locus_tag="KLH32_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBE0138774.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8027..8524
                     /gene="rplJ"
                     /locus_tag="KLH32_001934"
     CDS             8027..8524
                     /gene="rplJ"
                     /locus_tag="KLH32_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBE0138775.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8591..8956
                     /gene="rplL"
                     /locus_tag="KLH32_001935"
     CDS             8591..8956
                     /gene="rplL"
                     /locus_tag="KLH32_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBE0138776.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            9276..13304
                     /gene="rpoB"
                     /locus_tag="KLH32_001936"
     CDS             9276..13304
                     /gene="rpoB"
                     /locus_tag="KLH32_001936"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBE0138777.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13381..17604
                     /gene="rpoC"
                     /locus_tag="KLH32_001937"
     CDS             13381..17604
                     /gene="rpoC"
                     /locus_tag="KLH32_001937"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBE0138778.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KLH32_001938"
     CDS             complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KLH32_001938"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBE0138779.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KLH32_001939"
     CDS             complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KLH32_001939"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBE0138780.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KLH32_001940"
     CDS             complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KLH32_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBE0138781.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KLH32_001941"
     CDS             complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KLH32_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBE0138782.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KLH32_001942"
     CDS             complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KLH32_001942"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBE0138783.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KLH32_001943"
     CDS             complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KLH32_001943"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBE0138784.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(23291..23391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KLH32_001944"
     CDS             complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KLH32_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBE0138785.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24015..24788
                     /gene="nudC"
                     /locus_tag="KLH32_001945"
     CDS             24015..24788
                     /gene="nudC"
                     /locus_tag="KLH32_001945"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBE0138786.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            24828..25892
                     /gene="hemE"
                     /locus_tag="KLH32_001946"
     CDS             24828..25892
                     /gene="hemE"
                     /locus_tag="KLH32_001946"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBE0138787.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            26049..26720
                     /gene="nfi"
                     /locus_tag="KLH32_001947"
     CDS             26049..26720
                     /gene="nfi"
                     /locus_tag="KLH32_001947"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBE0138788.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            26763..27353
                     /locus_tag="KLH32_001948"
     CDS             26763..27353
                     /locus_tag="KLH32_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBE0138789.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            27540..27812
                     /gene="hupA"
                     /locus_tag="KLH32_001949"
     CDS             27540..27812
                     /gene="hupA"
                     /locus_tag="KLH32_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBE0138790.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            27825..28520
                     /locus_tag="KLH32_001950"
     CDS             27825..28520
                     /locus_tag="KLH32_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBE0138791.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KLH32_001951"
     CDS             complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KLH32_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBE0138792.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29185..30561
                     /gene="zraS"
                     /locus_tag="KLH32_001952"
     CDS             29185..30561
                     /gene="zraS"
                     /locus_tag="KLH32_001952"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBE0138793.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATLLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            30558..31883
                     /gene="zraR"
                     /locus_tag="KLH32_001953"
     CDS             30558..31883
                     /gene="zraR"
                     /locus_tag="KLH32_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBE0138794.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWSYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KLH32_001954"
     CDS             complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KLH32_001954"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBE0138795.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KLH32_001955"
     CDS             complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KLH32_001955"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBE0138796.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8384 a         8997 c         9570 g         8204 t
ORIGIN      
        1 ctacaaactc ttcctgtcgt catatctaca agccatcccc ccacagatac ggtaaactag
       61 cctcgttttt gcatcaggaa agcagctatg aaccactcct taaaaccctg gaacacattt
      121 ggcattgatc ataatgctca gcacattgta tgtgccgaag acgaacaaca attactcaat
      181 gcctggcagc atgcaaccgc agaaggacaa cccgttctta ttctgggtga aggaagtaat
      241 gtactttttc tggaagacta tcgcggcacg gtgatcatca accggatcaa aggtatcgaa
      301 attcatgatg aacctgatgc gtggtattta catgtaggag ccggagaaaa ctggcatcgc
      361 ctggtaaaat acactttgca ggaaggtatg cctggtctgg aaaatctggc attaattcct
      421 ggttgtgtcg gctcatcacc tatccagaat attggtgctt atggcgtaga attacagcga
      481 gtttgcgctt atgttgattg tgttgaactg gcgacaggca agcaagtgcg cttaactgcc
      541 aaagagtgcc gttttggcta tcgcgacagt atttttaaac atgaatacca ggatcgcttc
      601 gccattgtag ccgtaggtct gcgtctgcca aaagagtggc aacctgtact aacgtatggt
      661 gacttaactc gtctggatcc tacaacagta acgccacagc aagtatttaa tgcggtgtgt
      721 catatgcgca ccaccaaact ccctgatcca aaagtgaatg gcaatgccgg tagtttcttc
      781 aaaaaccctg ttgtatctgc cgaaacggct aaagcattac tggcgcaatt tccaacagca
      841 ccaaattacc cccaggcggg tggttcagta aaactggcag caggttggct tattgatcag
      901 tgccagctaa aagggatgca aatgggtggg gctgcggtgc accgtcaaca ggcgttagtt
      961 ctcattaatg aagacaatgc aaaaagcgaa gatgtggtgc aactggcaca ccatgtaaga
     1021 caaaaagtgg gtgaaaaatt taatgtctgg cttgagcctg aagtccgctt tattggtgca
     1081 tcaggtgaag tgagcgcagt ggagacaatt tcatgaagga taacaccgtg ccactgaaat
     1141 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaatgctgg
     1201 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg
     1261 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc catccagtta cttaatgctg
     1321 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtt attgactcca
     1381 cgaatcagtt ccttcttgat cgtatcggag agcttaaatc gggcgatgcc tgtgttgcag
     1441 aataccagca ggctggccgt ggtcgccggg ggcggaaatg gttttcgcct tttggcgcaa
     1501 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa
     1561 gtctggttat cggtatcgtg atggcggaag tattacgaaa gctgggagca gataaagttc
     1621 gtgtcaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc attcttgtgg
     1681 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg atcaacatgg
     1741 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg caggaagcgg
     1801 ggatcaatct tgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt
     1861 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata
     1921 attttattaa tcgcccagtg aaacttatca ttggtgataa agaaatattt ggcatttcac
     1981 gcggaataga caaacagggg gctttattac ttgagcagga tggaataata aaaccctgga
     2041 tgggcggtga aatatccctg cgtagtgcag aaaaataaga aaggggagta ttcgctcccc
     2101 tgcaaattat ttgcgtagtc tgacctcttc taccacatga ttagcacttt tcgtcaggat
     2161 taaactggcg cgctcacgag taggtagaat attttgcttt aagttcagcc agttgatctc
     2221 tttccacaat gtcatggcag tcttaatcgc ttcttcttta gttaatttcg cgtagttatg
     2281 aaaataggaa tccgggtctg taaaagcccc ttcgcggaat ttcagaaaac ggttgatata
     2341 ccacgtctga agtaagtctt ccggtgcatc aacatatatc gaaaaatcga caaaatcgga
     2401 aacaaataca tgatgtggat cgtgtggata atccatcccg ctctgtaaga catttaaccc
     2461 ttcaagaatt aaaatatcag gctgaacaac agttttatct ccatccggga tcacatcata
     2521 aataagatgc gagtaaacag gggctgtaac gtttggcacg ccggatttga gatcggaaac
     2581 aaacttcacc aggcgatgca tatcatacga ttccgggaag cctttcttct tcatcagacc
     2641 acgttctttc agaacctgat tagggtgaag gaagccatct gtagtgatca gttcaacacg
     2701 acgatgttcc ggccaacggc ttaatagcgc ctgcaataca cgggcggttg tacttttccc
     2761 caccgcgaca ctgccagcaa tactgataat gtaaggaatg cgttgcccgt tggtaccaag
     2821 aaactgttcc agaactgcct gacggcgcag attcgagctt atatagaagt tcagcaaacg
     2881 tgacaaaggt aaatagatct cggcaacttc ttctaacgag agatcttcat taataccttt
     2941 gagacgggcg atctcatctt ccgataacgt cataggtacg gaatcacgca gagctgccca
     3001 ctggttgcgg tcaaactgta ggtaaggcgt cattaacgtt tgctctttta tactcataag
     3061 catgtttctg gcggtcatag ctctatcggc aaagcgatat ttgccatggc gcgtcacagt
     3121 tctgttgaat gggttaatgg gcaggagggt aacaccagat ggggaggaat aataagaaaa
     3181 aatctctgcc aggaagctat cgttgaaaag caatgtgaca gatattccat ctgataaaaa
     3241 gaattatggt ttagcaggag cgcattgttg agcacaatga tgttgaaaaa gtgtgctaat
     3301 ctgccctccg ttcggctgtt tcttcatcgt gtagcataaa atgtgaccaa taaaacaaat
     3361 tatgcaattt tttagttgca tgaactcgca tgtctccata gaatgcgcgc tacttgatgc
     3421 cgacttagct cagtaggtag agcaactgac ttgtaatcag taggtcacca gttcgattcc
     3481 ggtagtcggc accatcaagt ccggtggggt tcccgagcgg ccaaagggag cagactgtaa
     3541 atctgccgtc acagacttcg aaggttcgaa tccttccccc accaccaatt tcggccacgc
     3601 gatggcgtag cccgagacga taagttcact taccggctcg aataaagaga gcttctctcg
     3661 atattcagtg cagaatgaaa atcaggtagc cgagttctag gatgcgggca tcgtataatg
     3721 gctattacct cagccttcca agctgatgat gcgggttcga ttcccgctgc ccgctccaag
     3781 atgtgctgat atagctcagt tggtagagcg cacccttggt aagggtgagg tcggcagttc
     3841 gaatctgcct atcagcacca cttcttttct cctccctgtt ttttccttct gtttattgca
     3901 ttcaacaagt cgggcatgtt gcctggttga tgtggtgata tcaccgattt atccgtgtct
     3961 tagagggaca atcgatgtct aaagaaaagt ttgaacgtac aaaaccgcac gttaacgtcg
     4021 gtactatcgg ccacgttgac catggtaaaa caacgctgac cgctgcaatc actaccgtac
     4081 tggctaaaac ctacggcggt gctgctcgcg cattcgacca gatcgataac gcgccggaag
     4141 aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc ccgacccgtc
     4201 actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg atcaccggtg
     4261 ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg atgccgcaga
     4321 ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc gtgttcctga
     4381 acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg gaagttcgtg
     4441 aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt ggttctgctc
     4501 tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg gctggcttcc
     4561 tggattctta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
     4621 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggca
     4681 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
     4741 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg gcgtgctggt gagaacgtag
     4801 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
     4861 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
     4921 aaggcggtcg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
     4981 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
     5041 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
     5101 caatccgtga aggcggccgt accgttggtg cgggcgttgt agcaaaagtt ctgagctaat
     5161 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
     5221 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
     5281 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
     5341 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
     5401 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
     5461 ttgctcctgg cggcgattgt cggcaactac ctttatcgcg acattatgct gccgctgcgt
     5521 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     5581 ggtaaagcta ccattgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     5641 ccgattcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     5701 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     5761 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     5821 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     5881 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     5941 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     6001 cgacgcgagc tggcacttgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     6061 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     6121 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     6181 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     6241 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     6301 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     6361 cgtgagattg gaatacaatt tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat
     6421 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     6481 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     6541 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     6601 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     6661 tacgctgacc gttctttcac tttcgttacc aagaccccgc cggcagcagt tctgctgaaa
     6721 aaagcggcag gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     6781 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     6841 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     6901 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgtgagaa agttgatgca
     6961 accaaacagt acgacatcaa cgaagctatc gcactgctga aagagctggc gactgctaaa
     7021 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     7081 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     7141 tttacccaag gtgcaaacgc tgaagctgct aaagctgcag gcgcagaact ggtaggtatg
     7201 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     7261 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg tggcctgatg
     7321 ccaaacccga aagtgggtac tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa
     7381 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     7441 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     7501 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     7561 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     7621 tacgtgggcg gtgattttgt ctacaatctt acccccacgt ataatgctta atgcagacgt
     7681 atatccgaga tattcgggtt gtggcaaggc ggcaactgag tgagtcgcca agagcatagc
     7741 taactatgtg actggtgcga atgaaggaag ccaacgccgc cacaagttga atagcgacgg
     7801 atagaaaaga tttgttcgtt ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga
     7861 gtttcgcaag aaacttaatc ccctgcgtag acggtgacag aacgctaaga ttattctttt
     7921 atattctggc ttgtttctgc tcaccgtaat taagacgctc tctccgtttg gaggagtgaa
     7981 gtgagttcca gagattttct ctggcaaaca tccaggagca aagctaatgg ctttaaatct
     8041 tcaagacaaa caagcgattg ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc
     8101 agtagttgcg gattcccgtg gcgtaactgt agataaaatg actgaactgc gtaaagcagg
     8161 tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga
     8221 aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt ccgaccctga ttgcatactc
     8281 tatggaacac ccgggcgctg ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa
     8341 atttgaggtc aaagccgctg cctttgaagg tgagctgatc ccggcgtctc agatcgaccg
     8401 cctggcaact ctgccgacct acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga
     8461 agcttcggct ggcaaactgg ttcgtactct ggctgctgta cgcgatgcga aagaagctgc
     8521 ttaatcgcag ttatcttttt aacgcattcg cttacgtata aacttattct gatattcagg
     8581 aacaatttaa atgtctatca ctaaagatca aatcattgaa gcagttgcag ctatgtctgt
     8641 aatggacgtt gtagaactga tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc
     8701 tgctgtagct gtagctgctg gcccggttga agctgctgaa gaaaaaactg aattcgacgt
     8761 aattctgaaa gctgctggcg ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac
     8821 tggcctgggt ctgaaagaag ctaaagactt ggtagaatct gcaccggctg ctctgaaaga
     8881 aggcgtgagc aaagacgacg cagaagcact gaaaaaagct ctggaagaag ctggcgctga
     8941 agttgaagtt aaataagcca acccttccgg ttgcagcctg agaaatcagg ctgatggctg
     9001 gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt
     9061 tgactactgc tgtgcctttc aatgcttgtt tctatcgacg acttaatata ctgcgacagg
     9121 acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg
     9181 gaaagtgttc cattttccgg tcaacaaaat agtgttgcac aaactgtccg ctcaatggac
     9241 agatgggtcg acttgtcagc gagctgagga accctatggt ttactcctat accgagaaaa
     9301 aacgtattcg taaggatttt ggtaaacgtc cacaagttct ggatgtacct tatctccttt
     9361 ctatccagct tgactcgttt cagaaattta tcgagcaaga tcctgaaggg cagtatggtc
     9421 tggaagctgc tttccgttcc gtattcccga ttcagagcta cagcggtaat tccgagctgc
     9481 aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg
     9541 gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag
     9601 cgccggaagg caccgtaaaa gacattaaag aacaagaagt ctacatgggc gaaattccgc
     9661 tcatgacaga caacggtacc tttgttatca acggtactga gcgtgttatc gtttcccagc
     9721 tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg taaaacccac tcttcgggta
     9781 aagtgctgta taacgcgcgc atcatccctt accgtggttc ctggctggac ttcgaattcg
     9841 atccgaagga caacctgttc gtacgtatcg accgtcgccg taaactgcct gcgaccatca
     9901 ttctgcgcgc cctgaactac accacagagc agatcctcga cctgttcttt gaaaaagtta
     9961 tctttgaaat ccgtgataac aagctgcaga tggaactggt gccggaacgc ctgcgtggtg
    10021 aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta
    10081 tcactgcgcg ccacattcgc cagctggaaa aagacgacgt caaactgatc gaagtcccgg
    10141 ttgagtacat cgcaggtaaa gtggttgcta aagactatat tgatgagtct accggcgagc
    10201 tgatctgcgc agcgaacatg gagctgagcc tggatctgct ggctaagctg agccagtctg
    10261 gtcacaagcg tatcgaaacg ctgttcacca acgatctgga tcacggccca tatatctctg
    10321 aaaccttacg tgtcgaccca actaacgacc gtctgagcgc actggtagaa atctaccgca
    10381 tgatgcgccc tggcgagccg ccgactcgtg aagcagctga aagcctgttc gagaacctgt
    10441 tcttctccga agaccgttat gacttgtctg cggttggtcg tatgaagttc aaccgttctc
    10501 tgctgcgcga agaaatcgaa ggttccggta ttctgagcaa agacgacatc attgatgtta
    10561 tgaaaaagct catcgatatc cgtaacggta aaggcgaagt cgatgatatc gaccacctcg
    10621 gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa ccagttccgc gttggcttgg
    10681 tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg cgatctggat accctgatgc
    10741 ctcaggatat gatcaacgcc aagccgattt ccgcagcagt gaaagagttc ttcggttcca
    10801 gccagctgtc tcagtttatg gaccagaaca acccgctgtc tgagattacg cacaaacgtc
    10861 gtatctccgc actcggccca ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag
    10921 acgtacaccc gactcactac ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca
    10981 tcggtctgat caactctctg tccgtgtacg cacagactaa cgaatacggc ttccttgaga
    11041 ctccgtatcg taaagtgacc gacggtgttg taactgacga aattcactac ctgtctgcta
    11101 tcgaagaagg caactacgtt atcgcccagg cgaactccaa cctggatgaa gaaggccact
    11161 tcgtagaaga cctggtaact tgccgtagca aaggcgaatc cagcttgttc agccgcgacc
    11221 aggttgacta catggacgta tccacccagc aggtggtatc cgtcggtgcg tccctgatcc
    11281 cgttcctgga acacgatgac gccaaccgtg cattgatggg tgcgaatatg caacgtcagg
    11341 ccgttccgac tctgcgcgct gataagccgc tggttggtac tggtatggaa cgtgctgttg
    11401 ccgttgactc cggtgtaact gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg
    11461 cttcccgtat cgttatcaaa gttaacgaag acgagatgta tccgggtgaa gcaggtatcg
    11521 acatctacaa cctgaccaaa tacacccgtt ctaaccagaa cacctgtatc aaccagatgc
    11581 cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt gctggcagac ggtccgtcca
    11641 ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg
    11701 gttacaactt cgaagactcc atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca
    11761 ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag
    11821 agatcaccgc tgacatcccg aacgtgggtg aagctgcgct ctccaaactg gatgaatccg
    11881 gtatcgttta cattggtgcg gaagtgaccg gtggcgacat tctggttggt aaggtaacgc
    11941 cgaaaggtga aactcagctg accccagaag aaaaactgct gcgtgcgatc ttcggtgaga
    12001 aagcctctga cgttaaagac tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta
    12061 tcgacgttca ggtctttact cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg
    12121 aagaaatgca gctcaaacag gcgaagaaag acctgtctga agaactgcag atcctcgaag
    12181 cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc
    12241 tcgacaaact gccgcgcgat cgctggctgg agctgggcct gacagacgaa gagaaacaaa
    12301 atcagctgga acagctggct gagcagtatg acgaactgaa acacgagttc gagaagaaac
    12361 tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct ggcaccgggc gtgctgaaga
    12421 ttgttaaggt atatctggcg gttaaacgcc gtatccagcc tggtgacaag atggcaggtc
    12481 gtcacggtaa caagggtgta atttctaaga tcaacccgat cgaagatatg ccttacgatg
    12541 aaaacggtac gccggtagac atcgtactga acccgctggg cgtaccgtct cgtatgaaca
    12601 tcggtcagat cctcgaaacc cacctgggta tggctgcgaa aggtatcggc gacaagatca
    12661 acgccatgct gaaacagcag caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt
    12721 acgatctggg cgctgacgtt cgtcagaaag ttgacctgag taccttcagc gatgaagaag
    12781 ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg
    12841 gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg cgacctgccg acttccggtc
    12901 agatccgcct gtacgatggt cgcactggtg aacagttcga gcgtccggta accgttggtt
    12961 acatgtacat gctgaaactg aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg
    13021 gttcttacag cctggttact cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc
    13081 gtttcgggga gatggaagtg tgggcgctgg aagcatacgg cgcagcatac accctgcagg
    13141 aaatgctcac tgttaagtct gatgacgtga acggtcgtac caagatgtat aaaaacatcg
    13201 tggacggcaa ccatcagatg gagccgggca tgccagaatc cttcaacgta ttgttgaaag
    13261 agattcgttc gctgggtatc aacatcgaac tggaagacga gtaattctcg ctcaaacagg
    13321 tcactgctgt cggggtaaaa cccggcagcg gattgtgcta actccgacgg gagcaaatcc
    13381 gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc
    13441 aaaattgctc tggcttcgcc agacatgatc cgttcatggt ctttcggtga agttaaaaag
    13501 ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt
    13561 atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac
    13621 cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag
    13681 cgtatgggcc acatcgaact ggcttccccg actgcgcaca tctggttcct gaaatcgctg
    13741 ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac
    13801 tttgaatcct atgtggttat cgaaggcggt atgaccaacc tggaacgtca gcagatcctg
    13861 actgaagagc agtatctgga cgcgctggaa gagttcggtg acgaattcga cgcgaagatg
    13921 ggtgcggaag caatccaggc tctgctgaag agcatggatc tggagcaaga gtgcgaacag
    13981 ctgcgtgaag agctgaacga aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt
    14041 atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac cagagtggat gatcctgacc
    14101 gttctgccgg tactgccgcc agatctgcgt ccgctggttc cgctggatgg tggtcgtttc
    14161 gcgacttctg acctgaacga tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa
    14221 cgtctgctgg atctggctgc gccggacatc atcgtacgta acgaaaaacg tatgctgcag
    14281 gaagcggtag acgccctgct ggataacggt cgtcgcggtc gtgcgatcac cggttctaac
    14341 aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta aacagggtcg tttccgtcag
    14401 aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg taatcaccgt aggtccatac
    14461 ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc tggagctgtt caaaccgttc
    14521 atctacggca agctggaact gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg
    14581 gttgagcgcg aagaagctgt cgtttgggat atcctggacg aagttatccg cgaacacccg
    14641 gtactgctga accgtgcacc gactctgcac cgtctgggta tccaggcatt tgaaccggta
    14701 ctgatcgaag gtaaagctat ccagctgcac ccgctggttt gtgcggcata taacgccgac
    14761 ttcgatggtg accagatggc tgttcacgta ccgctgacgc tggaagccca gctggaagcg
    14821 cgtgcgctga tgatgtctac caacaacatc ctgtccccgg cgaacggcga accaatcatc
    14881 gttccgtctc aggacgttgt actgggtctg tactacatga cccgtgactg tgttaacgcc
    14941 aaaggcgaag gcatggtgct gactggcccg aaagaagcag aacgtctgta tcgctctggt
    15001 ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac
    15061 ggtgaattag tagcgaaaac cagcctgaaa gacacgactg ttggccgtgc cattctgtgg
    15121 atgattgtac cgaaaggtct gccttactcc atcgtcaacc aggcgctggg taaaaaagca
    15181 atctccaaaa tgctgaacac ctgctaccgc attctcggtc tgaaaccgac cgttattttt
    15241 gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc gttctggtgc atctgttggc
    15301 atcgatgaca tggtcatccc ggagaagaaa cacgaaatca tctccgaggc agaagcagaa
    15361 gttgctgaaa ttcaggagca gttccagtct ggtctggtaa ctgcgggcga acgctacaac
    15421 aaagttatcg atatctgggc tgcggcgaac gatcgtgtat ccaaagcgat gatggataac
    15481 ctgcaaactg aaaccgttat taaccgtgac ggtcaggaag agaagcaggt ttccttcaac
    15541 agcatctaca tgatggccga ctccggtgcg cgtggttctg cggcacagat tcgtcagctt
    15601 gctggtatgc gtggtctgat ggcaaagccg gatggctcca tcatcgaaac gccaatcacc
    15661 gcgaacttcc gtgaaggtct gaacgtactc cagtacttca tctccaccca cggtgctcgt
    15721 aaaggtctgg cggataccgc actgaaaact gcgaactccg gttacctgac tcgtcgtctg
    15781 gttgacgtgg cgcaggacct ggtggttacc gaagacgatt gtggtaccca tgaaggtatc
    15841 atgatgactc cggttatcga ggggggtgac gttaaagagc cgctgcgcga tcgcgtactg
    15901 ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg ctgatattct cgttccgcgc
    15961 aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag agaactctgt cgacgcggta
    16021 aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg tatgtgcgca ctgctacggt
    16081 cgtgacctgg cgcgtggcca catcatcaac aaaggtgaag caatcggtgt tatcgcggca
    16141 cagtccatcg gtgaaccggg tacacagctg accatgcgta cgttccacat cggtggtgcg
    16201 gcatctcgtg cggctgctga atccagcatc caggtgaaaa acaaaggtag catcaagctc
    16261 agcaacgtga agtcggttgt gaactccagc ggtaaactgg ttatcacttc ccgtaacacc
    16321 gaactgaaac tgatcgacga attcggtcgt accaaagaaa gctacaaagt gccttacggt
    16381 gcggtactgg cgaaaggcga tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg
    16441 gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg
    16501 atcgacggcc agaccattac tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg
    16561 gttctggatt ccgcagaacg taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc
    16621 gttgatgctc agggtaacga cgttctgatc ccaggtaccg atatgcctgc gcagtacttc
    16681 ctgccgggta aagcgattgt tcagctggaa gatggcgtac agatcagctc tggtgacacc
    16741 ttggcgcgta ttccgcagga atccggcggt accaaggaca tcaccggtgg tctgccgcgc
    16801 gttgcggacc tgttcgaagc tcgtcgtccg aaagagccgg caatcctggc tgaaatcagc
    16861 ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc gtctggttat caccccggta
    16921 gacggtagcg atccgtacga agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa
    16981 ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt
    17041 ctgcgtctgc gtggtgttca tgctgtgact cgttacatcg ttaacgaagt acaggacgta
    17101 taccgtctgc agggcgtaaa gattaacgat aaacacatcg aagttatcgt tcgtcagatg
    17161 ctgcgtaaag ctaccatcgt taacgcgggc agctccgact tcctggaagg cgaacaggtt
    17221 gaatactctc gcgtcaagat cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca
    17281 acttactccc gcgatctgct gggtatcacc aaagcgtctc tggcaaccga gtccttcatc
    17341 tccgcggcat cgttccagga gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa
    17401 cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt
    17461 accggttacg cgtaccacca ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct
    17521 gcaccgcagg tgactgcaga agacgcatct gccagcctgg cagaactgct gaacgcaggt
    17581 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
    17641 cgggtttttt tatgggtgtt attcagaggg caaattcttc acggaaaata ttaagaaagg
    17701 cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cggcactacc
    17761 ataaaaaaac acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa
    17821 taaatttacg ttcttacatt ccgtctcata gtctttgcga ggcgcgtccc agatagctgt
    17881 cccagtcttt ccataccggc tgcaaaccct gagcggttaa cacggcagca accgcttccg
    17941 gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg gtgattatcg gcatagccac
    18001 ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc cagcggaata acgcgatcgc
    18061 gaaaccacgg tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagtcgga
    18121 aggcgcagat ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc
    18181 cagtacacgg gcgcaggcgc ggaaaggaga cagagtaacg gctttgccag taatgctgtt
    18241 gcagccatag caaatgttcg gcaaccatat agcagtcaac gcgccagttg tcggaaaggc
    18301 caattagcgc gccgaggcct atcttatcaa tccccgcacg ccccagccga tccggcgttt
    18361 ccagccgcca gaagaagtcc tgttttttgc ctttcaaatg atggcgggta taagtcgcct
    18421 cgtgatatgt ctcctgataa accatcaccc catccagacc aagttgcttt aactcggcgt
    18481 attccgtctc cgccagcggt tgcacttcca tttgtagtga agagaactgt tcacgcaggg
    18541 cggggagatg acgacgaaag taatccatcc ccactttcgc ctgatgttca ccagtgacta
    18601 atagcagatg ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt
    18661 catccagcgt tttgcgcttg atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt
    18721 tagcgcaaag attggaaaga taaagcggga cgtagaaact aactgtgttg ccaaatcgct
    18781 gacgggtcag acgctgcgcc tgttgggcca gttgttccag atagccactg gcggcaggcg
    18841 ataacagcgc catcatgtcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt
    18901 cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc
    18961 gattgctgaa ggttttcatg ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg
    19021 agcaaaataa ctgcggctgc ccggtccgga ctgacgtgcc agcaggccta cttctaccgc
    19081 cagacgaaat gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac
    19141 taacaccgcg tcggccccca tttccagcgc ctgcgtggca tggctgggaa cgccgatgcc
    19201 agcatcgaca accaccggaa ctgtggcctg ctggataata atctccagca tggcacgggt
    19261 ttccagtccc tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac
    19321 ttcttccaga cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc
    19381 ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg
    19441 ggcgtcaggg tgaatctcca attttagcca gtttgtgcct aacgcttcac gtgccagatg
    19501 ggcggcgaaa atggcttctt ccgctgtttt cgccccggaa gtattcggca gcagggccac
    19561 acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt
    19621 catcgccagt gtcaccagct ggctgccgga agcgcggatc gcctccacca tcagttgtga
    19681 agaagcgaat ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg
    19741 taacatctca accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga
    19801 tatgttgcgt ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg
    19861 cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca
    19921 tcggttgatc gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg
    19981 cggcgcaacg ccaggctgcg ccactggctc gacttaccgt cgaacagtcg gagttctccc
    20041 gcaggcgtct ctataccgct taataactta atggcttcca gtgcctgcaa agtgcccatc
    20101 accccgacca ccgggccaac cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta
    20161 tctggccaca ggcagcggta acacccctgc tcccagggcg gtgtcagtac catcaactga
    20221 ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca
    20281 ttaatctcct ggcgagtcgc catattgtcg gtacagtcga gcaccacatc ggcttgtgca
    20341 accgcatctt tcaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatgtcg
    20401 ggattcaact gtgtcagtcg ctgttggctg acctgcgatt tcgggctgtt gatatcttca
    20461 gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt
    20521 accagcgtcc cgacgccagc ccctgccagg tatagcgcag caggtgtacc cagcccgcca
    20581 agaccaataa tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg
    20641 tcgagcagaa tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc
    20701 aatttccagc aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac
    20761 gacggcgata ctgccgacac ccgttgctat caccgcaggc gcgcgtgcca gactgatacc
    20821 gccaatcgcc acggtgggat aatccgccag gcgctcaaca tgccgtgcca actgttccag
    20881 cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat
    20941 ataagagggg cgtgctgcca gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc
    21001 cagccgcagg cctgccgcgc ggatggcatt gagatcggtg gcttgcaaat cttcctgccc
    21061 caaatggacg ccatacgcct gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa
    21121 tcgcgcgtta tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc
    21181 atcgcgccga tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc
    21241 gatccactgt acgctgtcca ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa
    21301 atcaggctga tacatcacgc ttcctcctta tgcaggtaga tttctccgcc tctggcacgg
    21361 aagttctccg acatatccgc cattcccact tcaatggttt gcgcggcggc gtaatcgcgt
    21421 acttcctggc tgattttcat cgagcagaat tttggcccac acatggagca aaaatgggcg
    21481 actttgccgg actcttgggg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg
    21541 agggccagat taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta
    21601 tcgcgaattt gcgcgcccgg atgccctttt gccaggtcag cagcgtgggc ggcaatctta
    21661 taagtgataa gcccctgctt aacatcttct ttattgggca gacccagatg ctcttttggc
    21721 gttacgtaac agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac
    21781 gtgaagtggt catagcccgg tgcaatatcg gtagttagcg gccccagagt gtaaaacggc
    21841 gcttcgtggc agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcaca
    21901 tggcctgggc cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttcg
    21961 cccagcgtat gcaattcggc aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg
    22021 cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg
    22081 cggaagtgtt gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc
    22141 gaaccgccgc gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc
    22201 agtacgcccg catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg
    22261 tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg
    22321 tagatcggca ctgtaccgat cggcaccgga ctgttacgca aaatccactc gcgggtttcg
    22381 tgaatatagc gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat
    22441 accagctttt ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg
    22501 ttaactttta ccaggaagtt acggccaata atcatcggct ccgattccgg atgattaatg
    22561 ttggccggga taatcgcacg tccggcagca acttcatcac ggacaaattc cgcagtgata
    22621 ttttccggca gatgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacctcgcta
    22681 cggatgcgct cgcggcccat attctcgcgg atggcgatga attccatgtc cggcgtgatg
    22741 atgccctggc gggcgtagtg cagttgggtg acacggcgtc ctgttttggc gcgttttggc
    22801 gttaataagc cgctaaaacg tagctcgtcg agaccatcat ctgccagccg cgttctggtg
    22861 taatcggaac tgcgcacggt aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg
    22921 cgtagttttg ccagcccttg ctgcacgtta atggcgatct gcggatcacc atacgggccg
    22981 gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact gcggctgttc tttgctaccg
    23041 ccaattagcg tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg
    23101 ccagtgatat aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa
    23161 tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg ttgcagacat agctcattcc
    23221 aaaaagttaa ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag
    23281 gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg
    23341 cggatcccga ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagcccccac
    23401 aaacggggcg ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga
    23461 cgcgggcggc gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca
    23521 gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg
    23581 cggtttccag gctggaatcg tagtaatcca taatctgttg ggtattggct tcgagttgcg
    23641 gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct
    23701 cataaatact gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa
    23761 gggctttttc gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt
    23821 agtaagccac gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc
    23881 ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt
    23941 attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag
    24001 gtagtgcaag agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca
    24061 gccatgaaca aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt
    24121 tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt
    24181 taatacaaca gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg
    24241 ttgggctgtt tcaactggcc ggacgtggcg tgcaactggc ggagttttac cgatcgcata
    24301 aatactgtgg ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt
    24361 gtagccattg ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgccatcc
    24421 gtcgcgatga ttcgctcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag
    24481 tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga
    24541 tggaagagag cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt
    24601 ttcctcagtc tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg
    24661 acccgaaaga attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc
    24721 cgcccggcac cgtagcgcgc cgtctgatag aagatactgt ggcgatgtgt cgggcagagt
    24781 atgagtgatg atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta
    24841 aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga
    24901 tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcgggcg
    24961 attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc
    25021 gtcgctaccc gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga
    25081 tggggttagg gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct
    25141 gcaaagccga cgtcgataaa ctgccaattc cggacccgga agatgagctg ggttacgtga
    25201 tgaacgcggt gcgtaccatt cgtcacgaac tgaaaggcga agtgccgctg attggttttt
    25261 ccggcagccc gtggacgctg gcaacctaca tggtggaagg cggcagcagc aaagccttca
    25321 ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct gcacgctctg ctcgataaac
    25381 tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct caggcagtga
    25441 tgattttcga cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct
    25501 attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag
    25561 tcacgctgtt taccaaaggc ggcggacagt ggctggaagc catggctgaa accggttgcg
    25621 atgcgctggg cctcgactgg acaacggaca tcgccgatgc gcgccgccgt gtgggcaata
    25681 aagtcgcgtt gcagggtaat atggatccgt cgatgctgta cgcgccgcct gcccgcattg
    25741 aagaagaagt agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc
    25801 ttggtcacgg cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag
    25861 tgcatcgact gtctgaacag tatcaccgct aagagcctga tccatcaggc tattttattt
    25921 gccattttga ggccgggcag tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt
    25981 tccgcgctgt ccggcctcaa actgccttca acaattacgc ctgttgaacc aggctctaag
    26041 gagcgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct tcttctgtga
    26101 tctgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc gatgtcgggt
    26161 ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg
    26221 agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt ccaggttttc
    26281 tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg
    26341 atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc gtcgccagcc
    26401 attttggctt attggtggat gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta
    26461 aattcgaacc gctctccagc aaaccgggcg cgctggcccc actgatggat aaaggcgagc
    26521 agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc
    26581 atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc
    26641 tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct
    26701 atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt tgagaactca
    26761 acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg cagcatgtca
    26821 ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc tgccagcaaa
    26881 ccggttttgg tgatgggcaa atttaccgtc gtattctcga tctcatctgg gaaacgctga
    26941 ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa gaagcgattc
    27001 cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt
    27061 taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga
    27121 gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggctggt cgcgaaatgg
    27181 gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt cagtgggaaa
    27241 tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa ggccttaggg
    27301 cagacctgcg tgagtcgggt gagagcaata ttggtataat ttttcagcaa taagaccaga
    27361 aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg
    27421 tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga gagtgctatt
    27481 ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa ggataactta
    27541 tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc aaaacccagg
    27601 ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa gaaggcgatg
    27661 ctgtacaact ggttggtttc ggtaccttca aagtgaacca tcgcgctgag cgtactggcc
    27721 gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt
    27781 ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac agttttaacg
    27841 aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc
    27901 tgtttctcac tgcctgtagc cacaactctt cacttcctcc ctttaccgcc agtggatttg
    27961 ctgaagacca gggcgcggta cgcatctggc gaaaagacag cggcgataat gtgcatctgc
    28021 ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat cgctggcagg
    28081 gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg aacattcgcg
    28141 cgcgttttga cgatcgcggc gatctgagct ttatgcaacg tgaatccgat ggggaaaagc
    28201 agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag atccgccaga
    28261 ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg
    28321 aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat tcgcaggcga
    28381 tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg gcatggctgg
    28441 aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt cgctggcaac
    28501 ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc caccaccgca
    28561 gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag ccatcgcgat
    28621 atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac
    28681 cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg
    28741 cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt
    28801 ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg
    28861 tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac
    28921 cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt
    28981 attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac
    29041 gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc
    29101 ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa
    29161 agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct
    29221 aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg
    29281 acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa
    29341 ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg
    29401 catcatgcgc agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg
    29461 tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga
    29521 aaacagcttt attcccctca ggaaatgcag cagttacatc cgggagatga agaagcgtgg
    29581 cggcggatcg atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg
    29641 atgtttgctg ctggaatgta ccggatgcgc catatgcagc agtatgccgc gacaccacaa
    29701 gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga
    29761 aacaccctga ttattctctt cgccctggcg acgctcttgc tggcaagcgt attgtcattc
    29821 ttctggtatc gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa
    29881 gagaagctgg tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca
    29941 ctttcctcga ttaaaggact ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa
    30001 gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc
    30061 gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg
    30121 ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta
    30181 cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag
    30241 gtcctgttga atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc
    30301 gtgacggcca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga
    30361 attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc
    30421 accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag
    30481 gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg
    30541 cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca
    30601 ttagccactg cactattttg caggctttac tgcgcggctg gagctataac gtcgcgctgg
    30661 cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt
    30721 gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa
    30781 acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg
    30841 cactgaaaac tggggcgcag gattatctca tcaagccgct ggatttcgat aacctacagg
    30901 cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga
    30961 ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa
    31021 tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggcaccggta
    31081 aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa
    31141 cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa
    31201 aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg
    31261 gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc
    31321 gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg
    31381 tccggctgat tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc
    31441 gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac
    31501 ggcgggaaga tatccctctg ctggctggcc attttcttca gcgctttgcc gagcgtaatc
    31561 gcaaggcggt aaaaggcttt acgccacagg cgatggattt gctgatccac tacgactggc
    31621 cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg
    31681 aatatatttc cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc
    31741 aaagtcagga tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg
    31801 agaaaacggg cggcaacaaa accgaagccg cccgtcagtt agggatcacg cgcaaaacgc
    31861 tattggcaaa actgtcgcgt tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt
    31921 tccggcagaa gcaatcatcc cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg
    31981 cttctgccac ggtatgaccc agcgcggtga cgcacagtac gcgcccgccg ctggttacca
    32041 cctgctcgtc atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt
    32101 ccagcggcag gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag
    32161 ccatcaccac gctgagagaa gcacgttcat cccactctga cgttttctcg tccagtttgc
    32221 cttcacaggc cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct
    32281 gggtttccgg atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt
    32341 cgatcatcag gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt
    32401 tcacggttgg ccagatgata cgttccatgg tgcgctgatg aacttcatcg gttactaccg
    32461 gcgcggggga gtaagcgccc atcccgccgg tgtttggtcc gttatcttta tcgcctacgc
    32521 gtttgtgatc ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa
    32581 agctcgcttc ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa
    32641 aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca
    32701 cgataacgcc tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct
    32761 cacgcagata caccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga
    32821 tcttatggcg ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac
    32881 ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca
    32941 ccagcggcgc ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca
    33001 acagcgccgg gatatcggtc acgccgatag caacgttttg cagcgcgggt tccagcgcag
    33061 tgcctgcatt acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc
    33121 aggccagcgc gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca
    33181 ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc
    33241 ggcaatcact tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc
    33301 agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga
    33361 acctttcact tccaggcctt catcggcagc tttaataccg gcgattttcg cggagtacac
    33421 gcggctcatc tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc
    33481 attggatttc acgaatttcg ccactttcca gcagaacagc gcatcacgca gttcctgttc
    33541 ggtcggctga cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg
    33601 atcctgaacc agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc
    33661 ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc
    33721 ttcgctggcg gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc
    33781 ggtttccgca tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc
    33841 ggttttgtac gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg
    33901 gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc
    33961 cgcatcggta tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt
    34021 agcaacggag gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt
    34081 ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg
    34141 accggcggct tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc
    34201 aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt
    34261 tgcaagcgtc agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct
    34321 gctcttcacc acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg
    34381 gccgccgata tcgatgttct caaccgcatc ttccagcgag cagccttcac gagcaacagt
    34441 ctgggcgaac ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc
    34501 cataatggca tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag
    34561 ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac
    34621 cggcagacct ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc
    34681 gcgtgcggaa agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag
    34741 agcgcggcgg actggacgac gttgttgcat ggtaaatccc ctgggtttga ctattacaga
    34801 gagcgttagc tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat
    34861 ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat
    34921 ggtgatcaca attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt
    34981 gtggataact ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc
    35041 agtcattttt ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgccttc
    35101 atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga agaga
//
LOCUS       DAEEDS010000036        35019 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEEDS010000036 DAEEDS010000000
VERSION     DAEEDS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35019
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.036"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..835)
                     /locus_tag="KLH32_001956"
     CDS             complement(<1..835)
                     /locus_tag="KLH32_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0138797.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GP"
     gene            927..1013
                     /locus_tag="KLH32_001957"
                     /pseudo
     CDS             927..1013
                     /locus_tag="KLH32_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1418..1894)
                     /locus_tag="KLH32_001958"
     CDS             complement(1418..1894)
                     /locus_tag="KLH32_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBE0138798.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="KLH32_001959"
     CDS             complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="KLH32_001959"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBE0138799.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3329..4369
                     /gene="bioB"
                     /locus_tag="KLH32_001960"
     CDS             3329..4369
                     /gene="bioB"
                     /locus_tag="KLH32_001960"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBE0138800.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4366..5520
                     /gene="bioF"
                     /locus_tag="KLH32_001961"
     CDS             4366..5520
                     /gene="bioF"
                     /locus_tag="KLH32_001961"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBE0138801.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5507..6262
                     /gene="bioC"
                     /locus_tag="KLH32_001962"
     CDS             5507..6262
                     /gene="bioC"
                     /locus_tag="KLH32_001962"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBE0138802.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6255..6932
                     /gene="bioD"
                     /locus_tag="KLH32_001963"
     CDS             6255..6932
                     /gene="bioD"
                     /locus_tag="KLH32_001963"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE0138803.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7511..9532
                     /gene="uvrB"
                     /locus_tag="KLH32_001964"
     CDS             7511..9532
                     /gene="uvrB"
                     /locus_tag="KLH32_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBE0138804.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="KLH32_001965"
     CDS             complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="KLH32_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBE0138805.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10897..11041
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11029..12018
                     /gene="moaA"
                     /locus_tag="KLH32_001966"
     CDS             11029..12018
                     /gene="moaA"
                     /locus_tag="KLH32_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBE0138806.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12040..12552
                     /gene="moaB"
                     /locus_tag="KLH32_001967"
     CDS             12040..12552
                     /gene="moaB"
                     /locus_tag="KLH32_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBE0138807.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12555..13040
                     /gene="moaC"
                     /locus_tag="KLH32_001968"
     CDS             12555..13040
                     /gene="moaC"
                     /locus_tag="KLH32_001968"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBE0138808.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13033..13278
                     /gene="moaD"
                     /locus_tag="KLH32_001969"
     CDS             13033..13278
                     /gene="moaD"
                     /locus_tag="KLH32_001969"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBE0138809.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13280..13732
                     /gene="moaE"
                     /locus_tag="KLH32_001970"
     CDS             13280..13732
                     /gene="moaE"
                     /locus_tag="KLH32_001970"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBE0138810.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13869..14573
                     /locus_tag="KLH32_001971"
     CDS             13869..14573
                     /locus_tag="KLH32_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE0138811.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14778..15491
                     /locus_tag="KLH32_001972"
     CDS             14778..15491
                     /locus_tag="KLH32_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE0138812.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15527..16483)
                     /locus_tag="KLH32_001973"
     CDS             complement(15527..16483)
                     /locus_tag="KLH32_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBE0138813.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="KLH32_001974"
     CDS             complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="KLH32_001974"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBE0138814.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17721..18482)
                     /locus_tag="KLH32_001975"
     CDS             complement(17721..18482)
                     /locus_tag="KLH32_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE0138815.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADIMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18615..19025
                     /locus_tag="KLH32_001976"
     CDS             18615..19025
                     /locus_tag="KLH32_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBE0138816.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18987..20093)
                     /locus_tag="KLH32_001977"
     CDS             complement(18987..20093)
                     /locus_tag="KLH32_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0138817.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20104..21237)
                     /locus_tag="KLH32_001978"
     CDS             complement(20104..21237)
                     /locus_tag="KLH32_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0138818.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21230..22966)
                     /locus_tag="KLH32_001979"
     CDS             complement(21230..22966)
                     /locus_tag="KLH32_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0138819.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="KLH32_001980"
     CDS             complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="KLH32_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBE0138820.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="KLH32_001981"
     CDS             complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="KLH32_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBE0138821.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24857..26044
                     /gene="rhlE"
                     /locus_tag="KLH32_001982"
                     /pseudo
     CDS             24857..26044
                     /gene="rhlE"
                     /locus_tag="KLH32_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="KLH32_001983"
     CDS             complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="KLH32_001983"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBE0138822.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26853..29003
                     /gene="dinG"
                     /locus_tag="KLH32_001984"
     CDS             26853..29003
                     /gene="dinG"
                     /locus_tag="KLH32_001984"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBE0138823.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29031..29993
                     /gene="ybiB"
                     /locus_tag="KLH32_001985"
     CDS             29031..29993
                     /gene="ybiB"
                     /locus_tag="KLH32_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBE0138824.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30134..31219
                     /gene="hcxB"
                     /locus_tag="KLH32_001986"
     CDS             30134..31219
                     /gene="hcxB"
                     /locus_tag="KLH32_001986"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBE0138825.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31448..31708)
                     /locus_tag="KLH32_001987"
     CDS             complement(31448..31708)
                     /locus_tag="KLH32_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0138826.1"
                     /translation="MKTINTVVAAMALSTLSFGVFATEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="KLH32_001988"
     CDS             complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="KLH32_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBE0138827.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="KLH32_001989"
     CDS             complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="KLH32_001989"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBE0138828.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33032..35019)
                     /locus_tag="KLH32_001990"
                     /pseudo
     CDS             complement(33032..35019)
                     /locus_tag="KLH32_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8297 a         8979 c         9467 g         8276 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg
       61 acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg
      121 gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg
      181 gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg
      241 gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg
      301 gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag
      361 gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag
      421 gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag
      481 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      541 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      601 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      661 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      721 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      781 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt
      841 attgatcctc attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa
      901 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      961 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
     1021 cctacctttt catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg
     1081 agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa
     1141 gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg
     1201 gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag
     1261 ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcggt cagctttttt
     1321 atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa
     1381 gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc gcggtaatcg
     1441 atgcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggctcctt
     1501 catcgacatc aatacgctct acatccagcg cgtgaacggt aaaaatgtag cgatgggttt
     1561 cgcctttcgg cggcgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca
     1621 aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat
     1681 cagcgggtaa attaacaact acccagtgcc accagccgga gccggttggc gcatccgggt
     1741 cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg
     1801 gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat
     1861 cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa
     1921 gtgtagccag aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt
     1981 accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc
     2041 ggcatcaggt aaatcagttt gccaaaaggc cggatccata ccccctgttc gacaaagaat
     2101 ttttgcagcg ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt
     2161 acgcgcacat cggcaaccat ttcggcatca cgggctggtg ccagttgctc gcgcagctgc
     2221 acttcaatag ccgccacctg ctgctgccat tcgccggatt cgataatcgc taggctggcg
     2281 tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcagccg
     2341 gcttcgccgt tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc
     2401 attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat
     2461 tcacaggcaa acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc
     2521 aagatacctt cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg
     2581 cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga
     2641 tgcgccgcca tcaggcgggc aaaaccaacc atatcgcgtt catcccattc accatccatg
     2701 cggctttgcg gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc
     2761 attgagttgt ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg
     2821 cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt
     2881 ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt
     2941 tgcggcgtca ttgccaccag tttgcggcat agctcgatgg cgggcgcatg ggtgataccg
     3001 ccaaacatca catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg
     3061 tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg
     3121 tcagacaaaa ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag
     3181 gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc
     3241 ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagcctaca ccgaattaac
     3301 aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca
     3361 agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca
     3421 tcgccagcat ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg
     3481 agcttgtccg gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga
     3541 agccgagcgg ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc
     3601 aggatcgacg cgcttctgta tgggcgcggc atggaagaat ccccacgaac gcgatatgcc
     3661 gtacctggaa caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct
     3721 gggcacgttg agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg attactacaa
     3781 ccacaacctg gacacctcgc cggagtttta cggcaatatc atcaccacac gcacttatca
     3841 ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg
     3901 cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa
     3961 cctgccgacg ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc
     4021 gcttgccgat aacgatgacg ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg
     4081 gatcatgatg ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca
     4141 gactcaggcg atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct
     4201 gaccacgccg aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa
     4261 tccgcagcaa actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttggacaggc
     4321 gctgatgacc ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag
     4381 aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg
     4441 gtggcgcaag gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc
     4501 agtaacgatt atttaggttt aagccatcat ccgcaaatta tccgtgcctg gaagcagggg
     4561 gcggagcaat ttggcatcgg tagcggcggc tccggtcacg tcagcggtta tagcgtggtg
     4621 catcaggcgc tggaagaaga actggccgag tggcttggct attcgcgggc gctgctgttt
     4681 atctctggtt ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa agaggaccgt
     4741 attgttgccg accggcttag ccatgcctca ttgctggagg ctgccagttt aagcccgtcg
     4801 cagcttcgcc gttttactca taacgatgtc gctcatctgg cgcgactgct tgcttccccc
     4861 tgtccggggc agcaactggt ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg
     4921 ccactggcgg aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat
     4981 gcccacggca cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta
     5041 aaaccagaat tgctggtagt gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg
     5101 ctttgctcaa gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc
     5161 accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt
     5221 gatgaggggg atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga
     5281 gtacaggatt tgccgtttac gctggctgat tcatgcagcg ccatccagcc attgatcgtc
     5341 ggtgataaca gccgtgcgtt acaactggca gaaaaactgc gccagcaagg ctgctgggtc
     5401 acggcgattc gcccgccaac cgtacccgct ggtactgcgc gactgcgctt aacgctaacc
     5461 gctgcgcatg aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg caacggttaa
     5521 taaacaagcc attgcagcgg catttggtcg ggcagccgca cactatgagc aacatgcaga
     5581 tctacagcgc cagagtgctg acgccttact ggcaatgctt ccacagcgta aatacaccca
     5641 cgtactggac gcgggttgtg gacctggctg gatgagccgc cactggcggg aacgtcacgc
     5701 gcaggtgacg gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc
     5761 cgcagatcat tatctggcag gagatatcga atccctgccg ttagcgactg cgacgttcga
     5821 tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga
     5881 gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc
     5941 gttacccgaa ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt
     6001 tttaccgcca gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac atcatattca
     6061 gctcatcaca ctatggtttg atgatgcgct cagtgccatg cgttcgctga aaggcatcgg
     6121 tgctacgcat cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg
     6181 attgcaactg gcctggccgc aacaacaggg gcgatatcct ctgacgtatc atcttttttt
     6241 gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga
     6301 aaaccgtcgc cagttgtgca cttttacaag ccgcgaaggc agtaggttat cggacggcag
     6361 gttataaacc ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg
     6421 cgctggcgtt acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca
     6481 ccttcgcaga acccacctcg ccgcacatca tcagcgccca agagggcagg ccgatagaat
     6541 cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg
     6601 aaggtgctgg cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa
     6661 cacaggaaca actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg
     6721 cgatgttgac tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga
     6781 acgatgttac gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga
     6841 ttcccgcgcc gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa
     6901 ccggaaagta cataaacctt gccttgttgt agccattctg tatttggtta aattgcgagc
     6961 gagatcgcgt cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa
     7021 aaattttact tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg ggggataggg
     7081 gctggagaca gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac
     7141 gaggcaagcg agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa
     7201 atatctttta ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc
     7261 ttgccataaa actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga
     7321 taacccggta aggctggagt tttatctcgc cacagagtaa attttgctca tgattgacag
     7381 cggagtttac gctgtatcag aaatattatg gtgatgaact gtttttttat ccagtataat
     7441 ttgttggcat aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag
     7501 gtagcgactc atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca
     7561 gccagaggcg attcgacgtc tcgaagaggg gctggaagat ggcctagcgc accagacgtt
     7621 acttggcgtg actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca
     7681 gcgcccaacc atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat
     7741 gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca
     7801 gccggaagcc tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga
     7861 acatattgag cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtgat
     7921 tgtagtggcg tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat
     7981 gatgctccat ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc
     8041 ggagctgcaa tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg
     8101 cgaggtgata gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt
     8161 tgacgaggaa gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac
     8221 tattccacgt tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt
     8281 acaggcgatg gaggagatca aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa
     8341 caacaaactg ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat
     8401 ggacgagctg ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg
     8461 accgggtgag ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt
     8521 cgatgaatct cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg
     8581 taaagagaca ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct
     8641 taagtttgaa gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg
     8701 taattacgag ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg
     8761 attgcttgac ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc
     8821 ggagattcgt cagcgagcgg caattaacga acgcgtgctg gtcaccacac tgaccaagcg
     8881 aatggcggaa gatcttgccg aatatctcga agaacacggt gagcgcgtgc gttatcttca
     8941 ctcagatatc gacaccgtcg agcgtatgga gattatccgc gacttgcgtc tgggtgagtt
     9001 cgacgtattg gtagggatca acttactgcg tgaaggtctg gatatgccgg aagtgtcgct
     9061 ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca
     9121 gaccattggt cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataggag
     9181 cactccatca atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa
     9241 gtacaacgag gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct
     9301 ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat
     9361 tgttgagccg gataatgtgc cgatggatat gttgccgaaa gcgttgcagc agaaaatcca
     9421 tgaactggaa gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca
     9481 aattcgtgac cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag
     9541 cgaagaagac tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc aggcctacat
     9601 tttctccgca atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca
     9661 tccggcataa acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt
     9721 ccgttaacct aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg
     9781 acgatacgga atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc
     9841 cgagacatcc acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg
     9901 ctccataatt gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg
     9961 ccccagattg ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc
    10021 cttcagcagc agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag
    10081 atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa
    10141 taactcttga atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc
    10201 ctgatcgtca atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc
    10261 cactttcagc agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc
    10321 cagcgccttt aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccgccaaa
    10381 acggtattca aacatcgcgg aggcgacgtt cggttccgtt atcagctggt tgaggcagtt
    10441 gcgcatatcg ccccaagcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc
    10501 attatccgtg gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag
    10561 aacgcgtccc agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt
    10621 acgattgcgc atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg
    10681 gggaaacagc aattaacctg ccaatattga ggatacaaac tattttcttc tatctctaaa
    10741 ggacgatgca cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc cattttccct
    10801 tattctgtag cgaaatagca cgatcatgac gctatataca tgattacata gcgaaagtgt
    10861 ggatggtaaa aatctcattt acacgctagt atcggcttaa ccactaaaca ctctagcctc
    10921 tgcacctggg tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc
    10981 agggcgaagg aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa
    11041 ctgactgatg catttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt
    11101 aactttcgtt gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa
    11161 ggctttctta ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc
    11221 gaaaaagtgc gcctgacagg aggagagccg tctttacgcc gcgactttac cgatatcatc
    11281 gccgctgtgc gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt
    11341 ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc
    11401 gacagtttgg acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc
    11461 atggcaggga ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg
    11521 ctgatgcgtg atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc
    11581 cctatccagc tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt
    11641 aagcatcaca tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac
    11701 caattacgtc aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga
    11761 gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc
    11821 gtttcctcca ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc
    11881 gatctgctgg aaggcgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg
    11941 cgggagaaga aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta
    12001 tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa
    12061 tttatcccga cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat
    12121 acctccggtc actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa
    12181 gccattgtga aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc
    12241 gacgatgtac aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct
    12301 cccgaagcgc tgctgccgtt gttcgaccgt gaggttgaag gttttggtga agtgttccgt
    12361 atgttgtcgt ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc
    12421 gctaacaaaa cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg
    12481 gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat
    12541 ttgaagaaat aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg
    12601 tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca
    12661 tgcgcagcga gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg
    12721 ccactgcgcg tattgccggt attcaggcgg caaaacgcac ttgggatctg atcccgctgt
    12781 gtcacccgct gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc
    12841 gggtgcgtat agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt
    12901 taaccgcggc ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag
    12961 atatggtgat tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta
    13021 aggtggaagc ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca
    13081 gatgcaaccg aagtggctgc ggattaccca accgttgaag cgttacgcca gcacctggct
    13141 gcgcagagcg atcgctgggc gctggcgctg gaagatggca aattgctggc tgccgtcaac
    13201 cagacgctgg tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg
    13261 ccggtaaccg gaggttaaga tggcagaaac caaaattgtt gtcggcccgc agccgttcag
    13321 cgtaggagaa gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt
    13381 caccggaaag gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca
    13441 ctatccgggg atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg
    13501 gccgctgggg cgcgtcacgg tgattcaccg catcggggaa ttatggccgg gcgatgaaat
    13561 cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat
    13621 tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga
    13681 ccgctgggtt gaagcccggg agagcgatct gcaggcggca aaacgctggt agttttttgt
    13741 tagccggata aggcaccgct gcgtccggca atttcaccgc tcagtacatg cgtttacgct
    13801 gcgtcgatgc acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg
    13861 agatcgtcat ggacagattc ccacgttctg attcaatcgt acaaccccgg gctggcttgc
    13921 aaacttatat ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg
    13981 ttgcctggta tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct
    14041 ttttaatcgg tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc
    14101 aaaagctgag cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc
    14161 ttacgctttc cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg
    14221 ttactgccgg gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa
    14281 gtggctttgg caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca
    14341 acttctggct gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct
    14401 ttgtcggatt gacggcgtat gacactcaga aattgaaaaa tatgggtgag cagattgata
    14461 cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg
    14521 acttcatcaa cctgttcctg atgttgttac ggatcttcgg caaccgccgt taattctttt
    14581 tcaccatcac tatcccggcc atgtgtcggg atttttttct tactaaaaac gacacttaac
    14641 aagtaccagg tataaaatat gccttatgag cgagcggaat cgttttccgt atccataaat
    14701 acaaaagtat tttgtatgcg tggttttaaa tttattgatg gtgaatatta atgttttttc
    14761 taaggacaac taaaagaatg gaatcatact cgcaaaacag caataaatta gattttcagc
    14821 acgaggccag gatattaaac ggtatatggc tcattaccgc tttaggtttg gtggcaaccg
    14881 caggactagc ctggggagct aagtatctcg aaattacggc aaccaaatat gattcaccac
    14941 caatgtatgt cgccataggg ttattattac tttgtatgta tggcttaagt aaggatatca
    15001 acaagataaa tgccgccatc gcgggcgtaa tatatctgtt tttactctct ttggtggcga
    15061 ttgtcgttgc aagtttagtt cctgtatatg ccattatcat cgtgttcagc actgcgggcg
    15121 cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc
    15181 gttttatcat tatgatgacg ttgacagggt tggccctggt aatcatcgtg aatgcggcat
    15241 taatgagtga acggcccatt tgggtaataa gttgcttaat gattgtgtta tggccaggca
    15301 ttatctcgca tggacgaaat aagctccttg aacaggcagg aaaatgccat agtgaagagt
    15361 tgtggagtcc ggttcgttgc gcttttacag gtgcattaac actctattac tattttatcg
    15421 gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt
    15481 tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt
    15541 catttttcgc ccgtagcttc ttcgcttggc tttccagtag caggtaacag atcagcgcca
    15601 gcagcagcgg gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc
    15661 ctttcgaggt atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc
    15721 ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa
    15781 agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt
    15841 taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt
    15901 gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga
    15961 taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtaat
    16021 ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc
    16081 agttggtggt aatactgagc gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc
    16141 gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga
    16201 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
    16261 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga
    16321 ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt
    16381 ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga
    16441 ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcaggggt ttacccccgt
    16501 gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg
    16561 cgtcagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa
    16621 gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc
    16681 atccacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt
    16741 aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc
    16801 gagattactg gaccctactg tcgcccagtg atcgaccatc aatgccactt tgccgtggag
    16861 cgggcggcgg cggtactcaa aaacctgaat gccgccttta accagatagt tatacagcaa
    16921 gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg
    16981 caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta
    17041 ggcgttggcg ataatcactt cccgctgcgc ctgagtgagc attttcagat aatggcgttc
    17101 aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg
    17161 ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc
    17221 aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac
    17281 cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag
    17341 cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa
    17401 cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc
    17461 agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag
    17521 caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt
    17581 gccgacgtta tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc
    17641 aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt
    17701 gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa
    17761 aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg
    17821 tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg
    17881 tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt
    17941 tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc
    18001 acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt
    18061 acgcacatca catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg
    18121 ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa
    18181 cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca
    18241 aaatcgctcc acataatgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc
    18301 acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg
    18361 ctgacggtac gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta
    18421 aagcctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc
    18481 ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag
    18541 gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct
    18601 tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct
    18661 ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga
    18721 ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc
    18781 tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc
    18841 tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac
    18901 tggaaaacaa ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag
    18961 tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc
    19021 gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc
    19081 agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta
    19141 atccacgtca gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa
    19201 aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag
    19261 cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta
    19321 aagtagaaca gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc
    19381 agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag
    19441 atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg
    19501 cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc
    19561 gacggcacca caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc
    19621 tcgctgttgt tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc
    19681 tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac
    19741 tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc
    19801 agtagtaacg ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc
    19861 agcacatgtg taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg
    19921 ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac
    19981 gggaacagga tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg
    20041 cgcagcaacg actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc
    20101 tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga
    20161 agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag
    20221 gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac
    20281 cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc
    20341 ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag
    20401 ccccattccc agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga
    20461 accgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag
    20521 catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc
    20581 cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt
    20641 gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg
    20701 gcttatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt
    20761 ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc
    20821 ctgcacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt
    20881 ggcgttggcg cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat
    20941 tttccccgac tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat
    21001 gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag
    21061 caaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag
    21121 cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg
    21181 cgtctctttg acgcacagcg cccgcacgcg atgccaggaa aggatcgggt tactcattgc
    21241 tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg
    21301 gttgctcatc gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt
    21361 tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg
    21421 tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc
    21481 gggtgagggg gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca
    21541 tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt
    21601 gtgaggcaat acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg
    21661 cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt
    21721 tgccgtagag cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac
    21781 tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc agcaaaccgc
    21841 acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct
    21901 ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg
    21961 tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg
    22021 cacccagcgg cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa
    22081 aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt
    22141 ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccatcgc
    22201 tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg
    22261 tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca
    22321 gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg
    22381 aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt
    22441 cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt
    22501 cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg
    22561 ccaggcgtcc ggtaaacggc ccaagagacg tgaactccag caggcgagca aaagtttgct
    22621 tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg
    22681 tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc
    22741 cgtcgttttt gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta
    22801 atcccgccaa catccgcatc agtgtggttt tacctgcacc gtccggcccc accaaccccg
    22861 tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg
    22921 gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca
    22981 ccgaattgta ccgtcactgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca
    23041 atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta
    23101 aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca
    23161 tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca
    23221 taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg
    23281 agaaccgtgc ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac
    23341 gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg
    23401 gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc
    23461 tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca
    23521 tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca
    23581 tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg
    23641 cgatacccgg caagcatcag attatactgc gcctgtgcca ccgagacgcc cgctttcgcc
    23701 tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg
    23761 atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga
    23821 ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga
    23881 taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg
    23941 acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat
    24001 gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg
    24061 tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat
    24121 gggtatgaag gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc
    24181 gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg
    24241 tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga
    24301 gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag
    24361 gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa
    24421 taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt
    24481 aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca
    24541 ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac
    24601 ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt
    24661 aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc
    24721 acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt
    24781 gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg
    24841 gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc
    24901 cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgcggt
    24961 gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt
    25021 tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc
    25081 ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt
    25141 ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat
    25201 taacccacaa atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg
    25261 tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct
    25321 cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac
    25381 aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa
    25441 agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac
    25501 cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt
    25561 gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa
    25621 acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta gtgtggcgat
    25681 ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga
    25741 cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc
    25801 gcacgtcgtc aaccatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg
    25861 taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa
    25921 actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg
    25981 ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg
    26041 cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    26101 gtgaactctg caatatattg aatttgcatg cttttgtagg ccggataagg cgtttacgcc
    26161 gcatccggca tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat
    26221 ctgttttacc gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa
    26281 ctccattaaa aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta
    26341 agcatcgttt tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcacacg
    26401 cagttctgca tgctgttcga atttagcgcg aagtgcttta cgcatcacct gttctttaac
    26461 cgactcccag tttttacgaa gtggcttgga acgattacgc cccatgcgag cggcgaccat
    26521 gggggaagaa acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg
    26581 aaaatagtgt tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa
    26641 gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat
    26701 gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg
    26761 cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata
    26821 ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc
    26881 aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc
    26941 cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc
    27001 tggcgattga agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg
    27061 ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg
    27121 atcagattta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca
    27181 ctgccgcttt cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg ctcgccagca
    27241 ctgaacccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc
    27301 aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg
    27361 gtctgcgcga tcatacggat atcgctatag atgatgatct ctggcgtcgt ttgagtaccg
    27421 acaaagccag ctgcctcaac cgtaactgtt actactatcg tgaatgcccg ttttttgtta
    27481 ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg
    27541 cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag
    27601 gtcatcacct gccagatgtg gcccgggatg cgctggagat gagcgctgaa atcaccgcgc
    27661 cctggtatcg actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt
    27721 ttcgcccgaa gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag
    27781 agttgtatga gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc
    27841 aggaggcaga gcaccgtttt gcgatgggcg aactgcctga tgaattgctg gagatctgcc
    27901 agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt
    27961 taagtgagaa aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttacagatga
    28021 accgcgcgct ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc
    28081 aatcttccgg tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctac
    28141 acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc
    28201 gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaat agtttttcgc
    28261 gtttgcagga gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt
    28321 ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt
    28381 ccatcgacaa cgaagagcaa cacattgccg aaatggcggc ctttttccgt gaacaggtgg
    28441 agagcaaaaa atatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct
    28501 ttctcgacta tgtgacggat ttacgcctga tgttgctggt gcagggcgat cagccgcgtt
    28561 atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg
    28621 gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata
    28681 tccacaaaat cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc
    28741 tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc
    28801 tgattcagca ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct
    28861 acgacaaacg cttgctgaca aaaaattatg gcaagcgact actggatgca ttaccggtat
    28921 ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat
    28981 ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc
    29041 gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata
    29101 ctgcgcgcgg tctgtatgct catatgctca atggtgaagt ccctgacctt gagttgggcg
    29161 gcgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc ggcttttacg
    29221 aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg
    29281 tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta ttggcgattc
    29341 tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg
    29401 tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac ggcgggcagg
    29461 cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc
    29521 cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc
    29581 tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc
    29641 atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg tgggcgttac
    29701 taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc
    29761 tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt
    29821 tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt tgcctggctg
    29881 gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg
    29941 gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt taatttttat
    30001 ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa attcctgcat
    30061 ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa
    30121 acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat
    30181 tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag ttgccgatca
    30241 tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga tcccaagcta
    30301 tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa tcgttaaaga
    30361 ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga
    30421 agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca
    30481 taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag cggcggggtt
    30541 tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg
    30601 cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag ataatttccc
    30661 gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca
    30721 taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa
    30781 tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg aacataaagg
    30841 ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac
    30901 gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga ccactatcat
    30961 catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga
    31021 gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg gcgagtggga
    31081 agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg gaagctggca
    31141 ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc aggctttctg
    31201 tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac
    31261 ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg
    31321 tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta ggcctgataa
    31381 gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc cttatccgtc
    31441 ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag
    31501 tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc gcttccaggg
    31561 catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct
    31621 gggatgccgt taccggttcc gtcgcgaaca cgccaaatga cagagttgaa agagccatag
    31681 cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag
    31741 tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga
    31801 gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat
    31861 ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg
    31921 gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta
    31981 actgtctgtc tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt
    32041 tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga cgggcttgcg
    32101 ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga atttcacccc
    32161 gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt
    32221 tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc
    32281 taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc ccgcagcaga
    32341 ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc
    32401 gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc
    32461 gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc
    32521 aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg atgttgtcca
    32581 aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc
    32641 gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc
    32701 acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta
    32761 cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag cacctcattt
    32821 tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt aacttgtgca
    32881 ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga
    32941 aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc
    33001 caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga
    33061 aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat
    33121 cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc
    33181 ccagtttggc atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt
    33241 ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg
    33301 ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg
    33361 aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc
    33421 caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc
    33481 gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat
    33541 cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc
    33601 caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccac
    33661 tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt
    33721 taatatagac attgccgttt tccgtcaggt ggtacagcgc cccggctttc cagttcacca
    33781 gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc acaccagctg
    33841 ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat
    33901 gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat
    33961 cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca
    34021 ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt aacgtcaccg
    34081 gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat
    34141 gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat
    34201 tactcacatc tttggtattc gccgtgcgtg actaggtcca gctattcaca tcgctggtgg
    34261 gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc tgctttacgc
    34321 gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca
    34381 tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag
    34441 tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca gaatagcccg
    34501 gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat
    34561 taagatacaa acgatttgct gtaccaaggc caaaagcgac agaaggggcg acgccgtaac
    34621 gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc acattcaggc
    34681 gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg
    34741 cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca
    34801 tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc cctttaatca
    34861 cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg cgaataccat
    34921 caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg ccagtggtgg
    34981 agttaccgtt ctcacccgca aaaaacgcac ccacgccgg
//
LOCUS       DAEEDS010000037        34938 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEEDS010000037 DAEEDS010000000
VERSION     DAEEDS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34938
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.037"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..2261
                     /locus_tag="KLH32_001991"
     CDS             <1..2261
                     /locus_tag="KLH32_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBE0138829.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2258..3347
                     /locus_tag="KLH32_001992"
                     /pseudo
     CDS             2258..3347
                     /locus_tag="KLH32_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(3435..4733)
                     /gene="exuT"
                     /locus_tag="KLH32_001993"
     CDS             complement(3435..4733)
                     /gene="exuT"
                     /locus_tag="KLH32_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBE0138830.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5217..6629
                     /gene="uxaC"
                     /locus_tag="KLH32_001994"
     CDS             5217..6629
                     /gene="uxaC"
                     /locus_tag="KLH32_001994"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBE0138831.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6644..8131
                     /gene="uxaA"
                     /locus_tag="KLH32_001995"
     CDS             6644..8131
                     /gene="uxaA"
                     /locus_tag="KLH32_001995"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBE0138832.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8214..8765
                     /locus_tag="KLH32_001996"
     CDS             8214..8765
                     /locus_tag="KLH32_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBE0138833.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8770..10014)
                     /gene="sstT"
                     /locus_tag="KLH32_001997"
     CDS             complement(8770..10014)
                     /gene="sstT"
                     /locus_tag="KLH32_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBE0138834.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10610..11575)
                     /gene="alx"
                     /locus_tag="KLH32_001998"
     CDS             complement(10610..11575)
                     /gene="alx"
                     /locus_tag="KLH32_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBE0138835.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11846..12844)
                     /locus_tag="KLH32_001999"
     CDS             complement(11846..12844)
                     /locus_tag="KLH32_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0138836.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12923..13615)
                     /locus_tag="KLH32_002000"
     CDS             complement(12923..13615)
                     /locus_tag="KLH32_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBE0138837.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13688..14191)
                     /locus_tag="KLH32_002001"
     CDS             complement(13688..14191)
                     /locus_tag="KLH32_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBE0138838.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14276..15412
                     /gene="rlmG"
                     /locus_tag="KLH32_002002"
     CDS             14276..15412
                     /gene="rlmG"
                     /locus_tag="KLH32_002002"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBE0138839.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15697..16011
                     /gene="higB"
                     /locus_tag="KLH32_002003"
     CDS             15697..16011
                     /gene="higB"
                     /locus_tag="KLH32_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBE0138840.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16008..16424
                     /gene="higA"
                     /locus_tag="KLH32_002004"
     CDS             16008..16424
                     /gene="higA"
                     /locus_tag="KLH32_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBE0138841.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16469..18487)
                     /gene="fadH"
                     /locus_tag="KLH32_002005"
     CDS             complement(16469..18487)
                     /gene="fadH"
                     /locus_tag="KLH32_002005"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBE0138842.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18713..21064)
                     /gene="ygjK"
                     /locus_tag="KLH32_002006"
     CDS             complement(18713..21064)
                     /gene="ygjK"
                     /locus_tag="KLH32_002006"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBE0138843.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21081..21500)
                     /locus_tag="KLH32_002007"
     CDS             complement(21081..21500)
                     /locus_tag="KLH32_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138844.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21478..21831)
                     /locus_tag="KLH32_002008"
     CDS             complement(21478..21831)
                     /locus_tag="KLH32_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138845.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21832..22152)
                     /locus_tag="KLH32_002009"
     CDS             complement(21832..22152)
                     /locus_tag="KLH32_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138846.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22286..23719)
                     /locus_tag="KLH32_002010"
     CDS             complement(22286..23719)
                     /locus_tag="KLH32_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0138847.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23782..24231)
                     /locus_tag="KLH32_002011"
     CDS             complement(23782..24231)
                     /locus_tag="KLH32_002011"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBE0138848.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24228..27305)
                     /gene="ebgA"
                     /locus_tag="KLH32_002012"
     CDS             complement(24228..27305)
                     /gene="ebgA"
                     /locus_tag="KLH32_002012"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBE0138849.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27488..28471)
                     /gene="ebgR"
                     /locus_tag="KLH32_002013"
     CDS             complement(27488..28471)
                     /gene="ebgR"
                     /locus_tag="KLH32_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBE0138850.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28689..29020
                     /locus_tag="KLH32_002014"
                     /pseudo
     CDS             28689..29020
                     /locus_tag="KLH32_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29062..30441)
                     /gene="ygjG"
                     /locus_tag="KLH32_002015"
     CDS             complement(29062..30441)
                     /gene="ygjG"
                     /locus_tag="KLH32_002015"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBE0138851.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30859..32379
                     /gene="aer"
                     /locus_tag="KLH32_002016"
                     /pseudo
     CDS             30859..32379
                     /gene="aer"
                     /locus_tag="KLH32_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32533..33156)
                     /gene="nfeR"
                     /locus_tag="KLH32_002017"
                     /gene_synonym="yqjI"
     CDS             complement(32533..33156)
                     /gene="nfeR"
                     /locus_tag="KLH32_002017"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBE0138852.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33433..34178
                     /gene="nfeF"
                     /locus_tag="KLH32_002018"
                     /pseudo
     CDS             33433..34178
                     /gene="nfeF"
                     /locus_tag="KLH32_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34232..34307)
                     /locus_tag="KLH32_002019"
     tRNA            complement(34232..34307)
                     /locus_tag="KLH32_002019"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34272..34274),aa:Ile,seq:cat)
     gene            34432..34938
                     /gene="mug"
                     /locus_tag="KLH32_002020"
                     /pseudo
     CDS             34432..34938
                     /gene="mug"
                     /locus_tag="KLH32_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8539 a         8974 c         9174 g         8251 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caagcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctaatctat cgtcagggag
     3061 ggaccaccac cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa
     3121 ccggtgggca aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga
     3181 aacgtgtgcg tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac
     3241 ccttgactct ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg
     3301 gcaaattgtc agtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg
     3361 acaaacgctt ttttctatat aaacgacgaa gccgcctgat aaggcggctt cgtcgttata
     3421 acttacgtta aacgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca
     3481 atcgccggtt tgttctgcaa tacggtccag ataaccagcg cacccagcag gtcaaatact
     3541 gccagaactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc
     3601 gcaaacagcg tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact
     3661 tcgttacgac cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtgg
     3721 gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc
     3781 atacccgggc caatcatcag cacagcaccc agcgttacga ccatcttacg ggaaacgatc
     3841 aggttcacac caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg
     3901 aggtcagcaa acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg
     3961 taaactttaa acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgca
     4021 aggaaacgcg gcagtgcgat accccagaac tgacggttac gcaggatctg accaacggac
     4081 attttcttcg ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt
     4141 tcttcatcgg tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc
     4201 gcccagataa agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg
     4261 attgcccata ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag
     4321 tagcctactg cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc
     4381 gccgggatca tcgctgcttc cgcggcaccg accgcaccac gagcaacagc taggccaccc
     4441 cagctacctg ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca
     4501 taaccgattt tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta
     4561 taagcagcag aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct
     4621 tccatcagag ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg
     4681 ccgagcgtca ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac
     4741 ctcatcgttt tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg
     4801 ttagttacga ccgggtaacc gaaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt
     4861 ttcgtgctaa ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg
     4921 gcaatttgca ttccgtcgac ttatgatttg cgacggcaga aagataactt gtcatacaac
     4981 cttaaaaggt gagagccatc acaaatgtgc gaatatttgt agggacatta cctgacgaca
     5041 gcgaggccag tactggcgcg gcctgcagcg agatttacca ctttgagagt aattttttta
     5101 actacgttta ttgatctaac tcaagaaaat atcttcggac tctggaaatt ggtgtgataa
     5161 ctttgtcagc atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga
     5221 ctccgtttat gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg
     5281 actacgcaaa agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg
     5341 cggaagacta tcgttttaaa aacctgtatg acatctggtt gaagggcgat cactacaaat
     5401 ggcgcgctat gcgtaccaac ggtgtggctg agcgtctgtg tactggtgat gcgtctgacc
     5461 gtgaaaaatt tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc
     5521 actggacgca cctcgaactg cgtcgtccgt tcggtatcac tggcaaattg ctttctccgt
     5581 caactgccga tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg
     5641 cgcgcggcat catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg
     5701 attctctgga gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc
     5761 cgagctggcg tccggacaaa gccttcaata tcgaacaggc gacctttaac gactacatgg
     5821 cgaagctggg cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgcct
     5881 tgaccaaacg tctggatcac ttcgccgctc acggctgtaa agtgtctgac catgcgctgg
     5941 acgtggtgat gtttgctgaa gcgaacgaag cggaactgga cagcattctg gcgcgccgtc
     6001 tggctggcga aaccctgagc gagcacgaag tggcacagtt caaaactgcg gtactggtgt
     6061 tccttggtgc cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc
     6121 gtaataacaa cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca
     6181 acgaccgtcc gatggcggaa gagttgtcta agctgctgag caagcagaac gaagaaaacc
     6241 tgctgccgaa aaccattctc tactgcctga acccgcgcga taacgaagtg ctgggcacca
     6301 tgatcggtaa cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt
     6361 ggtttaacga tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtc
     6421 tgctgagccg ctttgtcggt atgctgactg acagccgtag cttcctgtca tacacccgtc
     6481 acgaatactt ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag
     6541 caccggcgga catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc
     6601 gtgactactt cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat
     6661 ccatgcgctg gataacgtcg cggtcgcttt agcagatttg gctgaaggca cagaagtcag
     6721 tgtcgataac cagactgtta cgctgcgcca ggatgttgct cgtggacata aatttgcgtt
     6781 aacggatatc gcaaaagggg ccaacgtcat caaatatggc ctgccgattg gttatgcatt
     6841 ggcggatatt gcggcggggg aacacgtcca cgcccacaat acgcgcacga atctgagcga
     6901 tctggatcag tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcc
     6961 tgaagtgcag atctatcgtc gcgctaacgg cgatgtcggg gtgcgtaacg agctgtggat
     7021 cctgccaacc gtgggctgtg tcaacggcat cgcgcggcaa atccagaacc gtttcctgaa
     7081 agagaccaac aacgccgaag gtaccgacgg cgtgttcctc ttcagccaca cctatggctg
     7141 ctcacaactg ggcgacgatc acatcaacac ccgcaccatg ctgcaaaaca tggtgcgcca
     7201 cccgaacgcg ggcgcagtgc tggtgattgg tctgggctgt gaaaacaacc aggttgccgc
     7261 attccgtgaa acgttgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca
     7321 gcaggatgat gagatcgaag ccgggatcga gcatttgcat cagctgtata acgtgatgcg
     7381 caacgataaa cgcgagccag gcaaactcag cgaactgaag tttggtctgg agtgcggtgg
     7441 ttctgacggt ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt
     7501 gattgctaac ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca
     7561 gttgctgatg gaccattgcc gcgacgaagc aacgtttgaa aaactggtca ccatggtcaa
     7621 cgacttcaaa cagtacttta ttgcccatga tcagccgatc tacgaaaacc catcaccggg
     7681 gaacaaagcg ggcggtatca ccacgctgga agacaaatca cttggctgta cccagaaagc
     7741 gggttccagt gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccagggct
     7801 gaacttgtta agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg
     7861 ctgccatatg gtgctgttca gtactggtcg cggcacgcca tacggtggat ttgtgccgac
     7921 ggtgaaaatc gccaccaaca gtgaactggc ggcgaagaaa aaacactgga tcgactttga
     7981 cgcgggtcag ctgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacac
     8041 catcgttgag tttgccaacg gtaagcaaac ctgcaacgag cgtaacgact tccgcgaact
     8101 ggcgatcttc aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg
     8161 cgtttcataa tgtgaagcgc cgtttgcttt tccatacctg ctaaggattt atcatgaccg
     8221 cgtattggct ggcccagggc gtgggtgtca tcgcctttct gattggtatc acaacatttt
     8281 tcaatcgtga cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg
     8341 tacatttttt tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc
     8401 gtacattgat taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga
     8461 ctggcggaat tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggta
     8521 cgattgtcag tacctgggcg ctgttccgct gtaaagggct caccatgcgc tgcgtgatgt
     8581 ggttttcaac gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacga
     8641 tgattgaggg tagttttctg cttatgaatg gcctgaatat cattcgtttc tggcggatgc
     8701 agaaaagggg aattgatccg tttaaagtag agaaaacccc tcccgccata gacgaaaggg
     8761 gttaaacaat taattacgca gggcgctatt tgccagacga tcgtcttccg cctggcaagc
     8821 tgccgcagtg aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa
     8881 tacgccgata ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa
     8941 catattacag gccagtggga tcagcagcaa agaacccccc gccacgccgg atgcgccaca
     9001 ggcacacaga gaagccacta cgctcaacag cagcgctgtg ggcagatcga ccggaatacc
     9061 cagcgtatta accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt
     9121 gatggtggct cccagcggaa tagaaactga ataggtatcg cgatccagat tcagcttttc
     9181 acacagcgcc atattcaccg gaatgttcgc tgcagagctg cgggtgaaga aggcatacac
     9241 accgctttcg cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca
     9301 caccagcaat gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag
     9361 cagttgcgcg tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag
     9421 cccaaaaata ccgaacggtg cgaagcgaat gaccagtttc accataaagg taacggcatt
     9481 cgacatatca ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaagccgag
     9541 gccgatcgcc cacaccagaa tcccgatgta gttacctttc agcagggcgt cgatggggtt
     9601 ggaaaccatg ctcattacca gtccgcgcat cacttcgaca atgcctgacg gcggcgaaat
     9661 atcacccgcg ctactggaca agtgcagggt agaagggaag gcaaagctga agactactgc
     9721 ggccagagct gcagagaagg tgcccagcag atagaggaac aaaataggac ggatattggt
     9781 tttctgcccg tgctggtggt tagcaataga tgccattacc agcatcaaca ccaggatggg
     9841 ggcaacagct ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc
     9901 gggttttgag atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac
     9961 caggctgcca tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg
    10021 atcctttcat tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg
    10081 taacaaagtg tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg
    10141 ctggatttta aagagaggtc atgttttgca acttaatggt tgcatttatt taacataaac
    10201 agagggaagg actggctact aaggctgatt agataacata tgggcaaaac tatttggaat
    10261 atgagaacgg ttgcgctcct ttaacgtgtt atgccatata aagtcactgg cgcagggagc
    10321 gcgcaggggg cggccaatcg ccgccgcccc ctgcaccccc aggctctggc gaacaaaatc
    10381 gccgctgcgc ggttccctca gcctatccct tgcggctacc gggtcgggcg caaggtaacg
    10441 tccctgtaag atgcgccctc gccccggcct ccagggaacg cttcggcgat tttgacgcca
    10501 ccaacacccg tgcggtcttt ttgtaaagag ctataagccg attacataat taatagctca
    10561 cctttgtctg gaatgagtta tctctaggaa tggcttaata tacagcgaat cattctcccc
    10621 gctgcttatc atgccgataa ttcacccagg cgttgataat aaacgtcatc accagaatgc
    10681 caaacaccac gcccagcgag acggcgattg gaatatggta gaagtcgaca atcagcatct
    10741 tgataccgat aaacaccaga atcaccgcca ggccatattt gagcatcgag aaacgctctg
    10801 ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag gattgcaaac aggtttgagg
    10861 tcagcacaat gaacgggtca gtggtcacgg cgaagatagc cggaatgcta tccacggcaa
    10921 aaatcacgtc gctcaattcc accagaatca gtaccagcat cagcggtgtg gcgtacagca
    10981 atccattctt acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac
    11041 cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg
    11101 ccatcttcac gccggtaaac agcaggaagg caccgaagat atacaggatc cagtcgaact
    11161 gcgaaatcag ccagctgcct gtgaagatca tgatggtacg cagaacaatc gccccgagca
    11221 cgccatacac cagcacgcga cgttgtaatg ccgccggaac ggagaaatag ctgaacaaca
    11281 tcagccagac aaagacgtta tcgaccgcca gcgatttctc aatcagataa cccgtgagaa
    11341 aggccagggc ctgtggatcg gcaacggcgc gaccttcggt ttgcaccaga taccaccaga
    11401 aagcggcgtt aaacagtaac gacagcgtca cccagaccag cgaccaggcc gcagcctgtt
    11461 tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa caggtcgata gccagcataa
    11521 tggcgacaac aacagcgaat ccgccccata gcaacggcgt gccgacagta ttcatagaag
    11581 ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc agccgctgct ttttatgcat
    11641 agacctcgcc ttccggcaag gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc
    11701 tttcgggttg cccggtgacc ggatgtggtt tttcacacat cgtaatgacg atcgaccggc
    11761 aatgaagtta ctcccctttg caggtaacaa agtatgacag accattcagt cagtcaatgg
    11821 tctgttacat accttttaca ctgttttact ttggcaagta tagttttacg ctatctgccg
    11881 gaaaaatcac cccagtttgg cggcggatct cggtcagcag tttggctgtg atgcgactga
    11941 ccgccagccc tggatgatcc accagatgct catccaccag ggtagcgaac agctctgctt
    12001 cgtagagcat ggtattaata tgctgcggct gggtgagatc ctgcatctgg ctgccacgcg
    12061 gcacaaagca gactttctgg cattcagaca gtttttcaat caccagcgat cccgcttcgc
    12121 cctgaatctc gcttcccagg acagaatcac tgacctttga gtgctgcaag gtgacgctga
    12181 aatcaccgta atccatcacc accacgccgt gggcgtcaac gccgcttgcc agcaaactcg
    12241 ccgttgcctg cacgcttttc ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa
    12301 agccgatatc cataattgaa ccgttagaga atgccggatt aaaggtgttg ggattctcac
    12361 cgtccaggta acgttgatac cgcgaggaat actggcaata gttgaaaaag actttacgca
    12421 gttttcctgc tttcggcagc gcctggcgca acaaatgaaa gttcggcagg caggcggttt
    12481 taaatgcctc aaacagcacc acctgatttt cgcgcgcaca ggcaatggcg gcatccactt
    12541 ccgccagatt cgacgccagc ggtttttcgc aaatcacatg aattttatgg ctaaggaaaa
    12601 gttgtgtctg ggaaaagtgc agggaattcg ggctggcaat atacaccgcg tcaatggcat
    12661 cgctttccgc catcgcttcc agcgacgtaa acagatgctc gacagaaaaa tcattggcga
    12721 agtgctgagc ctgctcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct
    12781 catgggcggc ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta
    12841 tcataacgcg tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat
    12901 cttcgacgtg cgcagatacg cattattctt tcgccggaca gccatgctca ctaaacggcc
    12961 caatcatgac cgatggcccg agatatttcg gctggcgatg gaggattttt gcgtcaatca
    13021 ccgcgctgac acgagcgagt ttctcccgca gttgagtata aaaaccgtgc ttcatattga
    13081 ggtctggcgc gttaaacccg atagcgtcga tgccgtactg ttttgccagc cagatggcgc
    13141 gttggttatg aaattcttgc gagataatgg tgatatggtt ttccccaaac acctttttgg
    13201 cacgcactac cgaatccagc gttgagaatc cggcatagtc gcaaaagatc actttcgccg
    13261 gtacgccttt agcgatcaaa gcctgctgca tacctgatgc ttcatcgtaa tttttacgtc
    13321 cgttatcgcc actcaccagt agccatttca cttttccggc gtggtacaac gctgctgcgg
    13381 tatcaatacg tcgggtaaag tagcgattac ccggcctcgc ccccagcaat aaacccacgt
    13441 tacgcgccgg gacggcgttg acatcgcccc aggtcagctg cttgctggcg ttgaccataa
    13501 cgcgatcggc gataaaaatg gtcgctcctg ccacaagtaa cagtaagcag gcaatcttta
    13561 gagtccggcg agaaaaacaa atacggagaa gaaggcgggc aaatgcgcgc agcatggtcg
    13621 ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta aagaaaagat aacgcctgac
    13681 atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga
    13741 actcaagctg gtggtactgc ggctccatat ggcagcacaa ttgatagaac gctttgttat
    13801 gctctttctc cttcaggtgc gccagctcgt gcaccacgat catgcgcaaa aacggttccg
    13861 gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct
    13921 gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt
    13981 catacatcac tttattgatc ggcggggcat tacgcagaaa ctgatttttc agatcctgag
    14041 tatattgcca gagagcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca
    14101 gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac ctgcgaaagc agctgctcgg
    14161 gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta
    14221 tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag
    14281 ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt
    14341 taacccgcta caggcgtggg aagcggcaga tgaatatttg ctgcaacagt tggacgacac
    14401 agaaatcccc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct
    14461 ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga
    14521 gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga
    14581 ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga
    14641 acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc
    14701 caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc tcggcccgac
    14761 caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca atgaaccgcc
    14821 gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca
    14881 taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct tctttatgca
    14941 acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta atggcgttat
    15001 tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc
    15061 gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttggatcg
    15121 cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt
    15181 gctctgcaac cctccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat
    15241 gttccactac gcccgccgct gcctgaaaat caacggcgag ctgtatatcg ttgccaaccg
    15301 ccatctggat tacttccata aactgaagaa aattttcggc aactgcacca ctattgcgac
    15361 gaataataaa tttgtggtgc tgaaagcggt gaagctggga cgtcgtcggt aagattggga
    15421 gttacctgcc ggatgcggcg taaacgcctt atccggccta cgttcagcac gttaccccta
    15481 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgctcagttc tggaatcgcg
    15541 cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgtttttc
    15601 tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt
    15661 atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca
    15721 ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac
    15781 acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg
    15841 gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt
    15901 gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat
    15961 aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg
    16021 ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc
    16081 agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc
    16141 ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc
    16201 ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc
    16261 gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat
    16321 ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat
    16381 tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg
    16441 cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg
    16501 caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag
    16561 ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga
    16621 tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt
    16681 catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcagga
    16741 tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc
    16801 gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc
    16861 caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct
    16921 ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac
    16981 aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca tcgagataac
    17041 tcaatacctt cggatgatcg atcccgtcga taggcggaac acgcggcacg atcccactgg
    17101 cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca
    17161 gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg cagcgtttcg taaaactcct
    17221 ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgcgcatcaa
    17281 acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc
    17341 cagcgccgac caccgccaga ttttttttct gcacggcggg aaggattggc attttggttt
    17401 cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga
    17461 ggcagccctg gttgcagcca atacaagtgt tgatctcatc ggctcgccct gattgcgctt
    17521 ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat cgatactata tcggcatcgc
    17581 cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg
    17641 gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg
    17701 gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat tccggtgttg ataatggtcg
    17761 cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc ggcaaaagtg ccgccgcctt
    17821 cgaccaggtc gagcatcgac aaccggtaaa taataatgaa gtcgttgccg acgcgttcgc
    17881 gcaccgtacg cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact
    17941 ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcagagc
    18001 ccatcacttc gacgccatcg tatccggcct cccgcgccag ttgcgcgcag cgggcgaaat
    18061 cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg
    18121 gggcctgcaa tgcggacggt gcgaccagat gcggttggta gctgtagcgc ccggtatgca
    18181 aaatttgcag ggcgattttg ccgccttcct gatgtacggc ttcggtaatg gtgcgatggt
    18241 gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat
    18301 ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa
    18361 acgcagccag ccgttcagca ccgtccgggt attcctccag cccggtgtgc attgaaccca
    18421 tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt
    18481 agctcataaa attgtccagt atgtaaaata attgttatgt agtcggatga gttctaattt
    18541 agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga
    18601 atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc ttgggggatt
    18661 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattactgct
    18721 tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag aaatttggtg
    18781 cgccttgctg cgcgcctgtc agcgggttgt aattctcctg aattgggcca tcggcggtta
    18841 atcctttggc gtgcctgaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt
    18901 aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccgcgccagt
    18961 agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcgga acaaaggtgt
    19021 tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg
    19081 caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc
    19141 ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag
    19201 tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc
    19261 gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc
    19321 tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc
    19381 cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag
    19441 cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacggc
    19501 ccgattccca cgacgcagcg acctgtgccg gaatttccag actgtcgtac tggcctttct
    19561 ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca
    19621 ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt gtcgcgggtc gcgccatatt
    19681 ccggcacccc gttgccgtta tgatcgcggt tacgtaacca ccagtcgtga taggccacca
    19741 gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtat acttccatca
    19801 ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac
    19861 cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac
    19921 gcacgctgtc gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga
    19981 tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct
    20041 gattgccgga gaaccagcgt ccggtcaccg acggcgtgac ggtgttataa ttcaccgcac
    20101 cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct
    20161 gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct
    20221 gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt
    20281 tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt
    20341 tgatatgcgc cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt
    20401 tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc
    20461 caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt
    20521 cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca
    20581 gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt tccagtagtg
    20641 acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg cacatctttt gctgtcagtt
    20701 tttgcaccag cgcgccggga atactgtatg cctccagcgt gaagttgact ttcttgccgt
    20761 cttgccagac agtcaggcgg tcgaaattgc tggccataaa gttgatgtat tcttccgtca
    20821 gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc gtctggcaac agatgaccat
    20881 gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct
    20941 tcatgtactg cggcgcacca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg
    21001 catgagcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata gttttgattt
    21061 tcatgagatt acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg
    21121 ggatcgttag aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg
    21181 ccgccatatt cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg
    21241 tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccttt taacgcttca
    21301 tacatggcgc tcaccatcat cccgctttca cttcccggta cgtccagccc gtcggcacgt
    21361 ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa acaggcccgt gagatagagt
    21421 tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg tgatgccttc ggcaaggtca
    21481 tactggatgt aaccgttcat ccattgccgg ataggtgtat ttgtcgtcgt agcgatcgta
    21541 cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt ccggcaggtg tggcggtgac
    21601 gctataacga aagttgccgc ttaagttctg taccttcagc gcggtcataa tgtcgcgggt
    21661 attgggaatg acgtagccca aatcgggtgt gaagtcgccc caccattgca ggtcatccag
    21721 cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca tcattggtac gataaccgcc
    21781 atagaaacgg ttaatcccac cttcgaaacc accccagcta tggtcaggca cctaggcatt
    21841 accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa atcaggccga accaggagcc
    21901 gtacttcatc tccaggccac cttcaatata ggtgccgtcg ctgtatttat gtttattctc
    21961 accctctaaa tccatatatc caccaagccc taattcgccg taaaaacgaa atgaaggaga
    22021 agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt tctgccggac gtgcttcttc
    22081 tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt aatgctctgc atggtgcagt
    22141 gattaatttc attgtgttaa tccatgagct aaagaaaaag tacagccttt tatcgttgta
    22201 attataaaaa tataaaggca gaaactattt tccctctcct catccaggaa gagggaaaat
    22261 gcctgctcat tttttattag tctgcttaat caacattgct ggctggcgtc gcttcaatat
    22321 ggcgctcttc agccgttaat ccctttatat atttgctgta tttccaccac gcaaagccga
    22381 ggaagataac aataccgccg acgttataaa aaatgatggt cagaatattc gcgccagtcg
    22441 ggaatgtcga agcgacaaac cctacggcaa atatcgcaat cagcattgaa acaacgataa
    22501 taccggtgcg gcgggagccc atacggaaat cgcgtggcag gtgatctaat ttggcgcgta
    22561 aattcaggta agccagcatg atgaataacg gcggaagcat ggacgctgcg gcggtcatat
    22621 taataatggt attcatcaga tcctgcacgg tattggaacc gagcatcggg ataatcatca
    22681 gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc cggaacgccg ttttcgttaa
    22741 gttcgacggt tttcttacca aagatgcctt ccgggatttc ggagaagaaa attttcaccg
    22801 gcgttgcggt ccacatcagc agggaaccga acattgcggt aaaggacacc agaccgacaa
    22861 agcgattcat caacgcttcc ggcagaccaa aatacgccgc catgccgtgg aatacctgca
    22921 ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa gacgttaatc agcaccgagg
    22981 agacggaata cagtacgccg ataaaaatcc cggcgaggat gatcacttta acgaacgatt
    23041 tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga ctccgcgcca cctgcggcca
    23101 taaagatcca ggtggtaacg ccgaggaacg cccagttgaa gttcgggatc atcgcgtcaa
    23161 cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc cgtacctgcc agtaaaatgt
    23221 aggagagcgt taacagcagc atcagcgttg aagtgacgga ggtaattggc cccagcattt
    23281 tcgccccgtt ggtggaaacc caggtggaga aggcgaacag caccatactg ataacggtgg
    23341 tggcaaccgg cgtcataata tattcgtagc cgaggaaggc atacgaagca taagcaataa
    23401 cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa ccagtaagtg taggcagtaa
    23461 taaatgccca acgtccgccc agcgaacttt ttacccacgc gtagacaccg gcttctgagt
    23521 ttttatttaa cgaaacaaat tctgcgatga tcagacagaa gggaataaaa taaaaaatcg
    23581 tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc aatattatta ttgataacgt
    23641 tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa gccgaatttg ccgattgtat
    23701 tacgtttggt atcagacata acacatctcc gaaaatgtgt agggtagcgg tggcggcaaa
    23761 ctccttttgc cgccgtagtt gttatttgtt atggaaataa ccatcttcga tggtgacttt
    23821 gagaaccact tttttgaccg cgttattgca gataaaccga tacgcttcat ggatatcgca
    23881 aataacgatt tgcccttcgt ggacctctac ggtttctccg cagcctttta aatattcacg
    23941 gtcagtttca tcacgataat attccactac ctgtaatgtc tctttcggcg catattcaat
    24001 tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag cgtcgatgcc cggtaaatag
    24061 cgcatcggtc gcggagtcat tttccacacg gtaggtcaat gaatcaccga tggagtgggc
    24121 gacgccaggc tgaatgttgt cgatattttc aatcgcttca acgcagcgtt gccacttctt
    24181 gccagaggcg taaatctggc ggaactgttc taagttatcg atgatcctca ttgcttattc
    24241 tcgctgtgca aattcgtgga aaagaaccct gcgccgaact catacgacgc caggctttgc
    24301 gcggtagctt ctccgccaga aaccggcagc aacgtaaagc cgtagctgaa gtcacggaac
    24361 cagacgcgcc aggagtccag cacctcgctg ccccaggagt tggagccgag gccaagtagc
    24421 tggtggtcga ggttcagggt gatgtcatca ctgcgctgca gctcgttaca gtgctgggca
    24481 gcgtggatgt tttcctgggt atagtgccag gcgctgaagt taattgggcg ctgcggaacc
    24541 accagcagac cgttgccgtg gcggttagtc agtgccgtcc agcggacatg ctgacggttg
    24601 ccgttgttct gcgggaaggg atagttctcg aacatggcat cgacggtgct gcgccagata
    24661 tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc ccggtccacg accgtaatac
    24721 gccacctgat cgtattcgcc gttaatcccc atggtgaaac cgatgcacgg aatgatgtgc
    24781 ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa cctggccatc ggcagcgatg
    24841 cgccagatgt aggtacagcg catcccgaag tcaaacaccg gcggtgcaat aactgtgcgg
    24901 ctgatgatca acacttcacc atcgctctgt tctacggcaa agtcgcgcag atgttcctgc
    24961 atgatctgca aatgattcgg ctgccacaac ccttcgtact cctgcttgtg gttgtcgatc
    25021 atcggcttga agaagttgat ctttggctcg cgggtcagca gcgactcgcc atttacctgg
    25081 caggatgtcg gtttgccact catttttgag aaggtgatcg cgaagttgta gccgcgaacg
    25141 gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg cattatttgg tgcgaaaggc
    25201 actggctgcg cggtgttttc cttcagcggg aactgataag aggcgataga atgcccggct
    25261 tcgctgtagc gggtgcggga atctttggtc accgtaatgt tgaggaacgc ttcgcgggcg
    25321 tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt tcggcgcaac gtcgcgcagt
    25381 ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca cctctgcgtg cagggtgtag
    25441 tcatcaagcg tggtaaacca cagtttattt tcgactttca actcgccgcg agtcagcgcg
    25501 tggattttta ccggcgcgat aacctgtttg tactctttca ggcccgatcc cggcgtctga
    25561 tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc gccgccgaat
    25621 ttataccaga cattgccgtg gtcgtcctgt gcctggatcc cgtggtcgca ccactcccag
    25681 acataatgac cctgaatgca atcgtgctta tagaagacgt tctggtactc cgtcagcccg
    25741 cccggtccgt tccccatcgc atgagcatat tcacagatga tgcgcggctt cggatgcggg
    25801 tattcaccaa actcattcat cagcggcacg cgggtgtaca tggtggaaat aatatcgacc
    25861 acttcagcat cgcgatcttc ttcgtaatga accagtcgcg tgtcatccag cgctttcgcc
    25921 gcatggtaca tcgcgcggat gttacagcca tagccggatt cattgcccag cgaccagatg
    25981 atgatcgacg gatggttttt ctgtgcgtgg atatggcgaa caatgcgctc gacgtagact
    26041 ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc aaagccgtgc
    26101 gattcgacgt cggtttccgc catcacaaac aggccgtaga tatcacacag ttcgtaaaaa
    26161 cgcggatcgt tcgggtagtg agcggtacgc acggagttga tattgtgctg cttcatcaac
    26221 tggagatctt tctcgacgcg atccattcca acggcgcggc ctttgcgatg atcgttgtcg
    26281 tgacggttga cgccgtgcag catcacataa cggttattga tccagaacag accgtcgcgc
    26341 actttgatat cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt gccgttggcg
    26401 tctttcagcg tcatgaccag atggtaaaga taaggggatt ctgctgacca ttgctgcggc
    26461 tgttcgacag taaaagcaaa gctggcgctg gtcagttttt caattgccaa atgatcaatg
    26521 gcgctgctgt gcaccacgcg ttcgccatca aacagggtat attccagcgt cgtgacgaca
    26581 ggggaggcgg cgagattttc cagcaccact tcgcaggaaa gcgtggcatc gcaataggct
    26641 tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt tccgaccaga
    26701 taaacatcgc ggaagatccc cgccgaccac cacatatcct ggtcttccac gtaggtagag
    26761 tccgcccact gcatcacgcg cacacacaac aggttgtcgc cggttttaac catcgcgctg
    26821 atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac ccacatactg accgttaacg
    26881 tagacttcaa aataggtttc gacgccgtca aatttaatca gcgtctgttt accctgccag
    26941 ccgtcgctga gggtgaaaat acgttgatag gcaccggttg ggttatcgct ggggacaaac
    27001 ggcacatcga tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc gtgaccttcc
    27061 atttgccaca tggcggggac ggtaatatgc ccccagtcag ccattaactc agaggtgaag
    27121 gcttctggta cttgcagcgg atggtcaaaa aagtggaaat tccactgact gcttaagggc
    27181 agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt gcgcaacaga atcatatgaa
    27241 aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt ttcccagcga
    27301 ttcatagggc ctcccggtag agagaaatcg gaaccattca tggtgatggt agtttgtgta
    27361 attgctgggg ggttagtaaa atttttacta aaaagcagca ggtaaatgca tttactttaa
    27421 ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa tgattgccgg acagtgtgta
    27481 aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag actagcagcg
    27541 gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga cttcccatca
    27601 tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg gtggggatat
    27661 cgttaacgct gataagcgaa atatcttgtg ggatgttcag gccacgttca tgaattgccc
    27721 gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga tagtcttccc
    27781 gcgccagcat ttgttttgcc agttcataac ccgacgaact ggaaaaaccg ccgcgccaga
    27841 tatcctcttc gcgtaccact tgtttcagtc ggccatattc cgcaaaggcg acctcacgaa
    27901 tatccgcctt gccaggctca tcttcaccgc caataaaacc aatacgatta acgccctggt
    27961 tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc accgcatcgt
    28021 aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac gcgctggcag
    28081 cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg tttttaatgt
    28141 ctggtaatcc gttgtgttca taacagttgg tcagttcgat cgccagcttt tcgcactgag
    28201 tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc tcctgctggt
    28261 agctgtagat agccagaata tggtgttggt tgactgcgcc tgtctgtagt ttacgggcgc
    28321 tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc gtctcttctt
    28381 tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg gatacgccag
    28441 cttcgattgc gatgtctttt aatgttgcca tgtttacctt ttttactttc cctgatgtgc
    28501 gcccccattg tagaggaagt gcttatcggg tcacctgttt tcagtaaata aagcgcgttt
    28561 ctttgacgct gtaacggtaa cacagcggct taggaatgtg atcacgcctg cattctccat
    28621 ttttagccta caggattgag aaaaactttt ctatagttta gtaaaatttt atccgaaggg
    28681 aggaagcaat ggaaaccgtg gcttacgctg attttgcacg tctggaaatg cgcgtcggaa
    28741 agattgtgga agtgaaacgc catgaaaacg ccgacaagct gtacatcgta caggttgatg
    28801 tggggaaaaa acactgcaaa ccgtgaccag cctggtgccg tactacagcg aagaggagct
    28861 gatggggaaa acggtggtgg tcttatgcaa tctgcaaaaa gcgaaaatgc gtggtgaaac
    28921 gtcggaatgc atgctgttgt gtgcggaaac cgatgacggc agtgaaagcg tgttgttaac
    28981 gccggagcgg atgatgcctg cgggcgtacg cgtcgtataa aaagatcgga tggcgacgtc
    29041 gtatcgccat ccgatttgat attacgcttc ttcgacactt actcgcatgg ccgccagcgc
    29101 cttacgcgcc gctttgatca ccagttcaca ctgttcaatg gtcagtgtca gtggcggttc
    29161 aatgcggatc gttttggcgt tattgagcgt tccggccacc agtacgcgct ggcggaacat
    29221 ctcgctggca aagttatagc cgatttcgtt atcaacaaac tcaatcgcca tcaacatccc
    29281 tttaccacgc gcttcctgta ccagatcggg atattcccgc gccagttgac ggaaaccgtc
    29341 cagcaacata tcgccttttt gctcagcctg agccggtaag ttctgctcca gcaacacatt
    29401 gatggtcgcc agcgccgccg cacaggccag cgggttgccg ccaaaggtgg tggtatgcag
    29461 gaatgggttg tcgaacagaa ctgaaaacac ctcttcagtg gcgatggtcg cgccaatcgg
    29521 catcacgccg ccgccgaggg cttttgccag acacagaatg tccggctgaa cgttctcatg
    29581 ttcgcaggca aacatcttgc ccgtgcgccc catgcccgtt tgtacttcat cgaggatcat
    29641 cagtgcgccg aactcatcgc acagcttacg cacggcggtg agatagcccg gcggcggcag
    29701 aattacgcca ccttcgccct gaatcggttc gaggatcacc gcagccacat catcaccggt
    29761 ttttttgcac tcattaagag ccgtgcgcat ggcttcgata ttgccaaacg gcacatgacg
    29821 gaaccctggc agcagtggca taaacggttt gcggaaggtc gatttcgccg tggccgacag
    29881 cgcgccaagt gatttaccgt ggaacgcgcc gctggtggca ataaaagtaa atttgccgcg
    29941 cggcgactga taagccttcg ccagcttcag cgccgcttca acggactcgg tgccgctatt
    30001 acagaagaag ctgtatttca gtttaccggg cgttagcgca gcaagggttt tcgccagcat
    30061 agcccgtaac ggatcgagta gctcctggct gtgcagtggt tgtttcgcaa gttgattctg
    30121 tacggcggaa accacaactg gattacggtg ccccacgttg aaaattccaa aacctcccag
    30181 gcagtcgata aactcctgtc cctgggtgtc gacaagcgta tttaaacctc ccgcttgcca
    30241 ctctacggct ccgtaatccc cgccggcggt aacagatttg cgatactcta aaaaccccgg
    30301 attgacatgc tctttgaagt attcaatcac ctctcggtta agtgctttca tctcctcatg
    30361 atccagcgtt cgcttctcaa tgagattcag ggcgtgggcg ctgcacgcta aagccgatgc
    30421 gctcgaaggt aacctgttca aaatatgctc cggaggctcg cgtatcacat gataccgatt
    30481 taagtattgc agggattgcg ccactccggc tgcgatcgcg aaaatcggga taaacacaaa
    30541 tgcggttatc atttgtgcaa tatttaatgc tatccgttaa catttgttgt tattttgcac
    30601 ggtttttgtc gggtatggat ttgatgattg ctgcgcaaat gcacgttttc agggcaataa
    30661 ttgcctgatt tttgggcaag ataattaacg ttttcgtata gtaacagagt gaaagtattt
    30721 taaatcaaag aattagattt gtgctttaat cgcaaattgc gatctaaatc aaattaatcg
    30781 gttaaagata accgcagcgg ggccgacata aactctgaca agaagttaac aaccatataa
    30841 cctgcacagg acgcgaacat gtcttctcat ccgtatgtca cccagcaaaa taccccgctg
    30901 gcggacgata ccactctgat gtccactacc gatctgcaaa gctatatcac tcatgctaat
    30961 gacacttttg tgcaggtgag cggctttacc ttgcaagagt tacaagggca gccgcacaac
    31021 atggtgcgtc acccggatat gccaaaagcg gcgtttgcgg atatgtggtt caccctgaaa
    31081 aaaggggagc cctggagcgg catcgtgaaa aatcgccgca aaaatggcga ccattattgg
    31141 gtgcgggcca atgcggtacc gatggtgcgc gagggaaaaa tcagtggcta tatgtcgatt
    31201 cgtacccggg cgacggatga agagattgcg gcggtggagc cgctgtacaa agcgttgaac
    31261 tccggacgta ccagtaagcg tattcataaa ggcctggtgg tgcgtaaagg ctggctgggt
    31321 aaactgcctt cattaccgct tcgctggcgg acgcgtggag tgatgaccct gatgtttatc
    31381 ttgctggcgg ccatgctttg gtttgttgct gccccggtgg tgacgtattt cctctgtgtg
    31441 ttagtggtat tgttggcaag cgcttgtttt gaatggcaga ttgtgcgccc gatagaaaat
    31501 gtcgcccgtc aggcactgaa ggtggcgacc ggagagcgta atagtgttga gcacctgaat
    31561 cgcagcgatg agctggggct gacattacgc gcggtagggc agcttggcct gatgtgccgt
    31621 tggctaatta acgatgtctc aagccaggtg tccagtgtca gaaatggcag tgagacgctg
    31681 gcgaaaggca ccgatgaact gaacgaacat acccagcaga cagttgataa cgttcagcaa
    31741 acggtggcga ccatgaacca aatggcggcg tcggtgaaac agaactctgc cacggcgtcg
    31801 gctgccgata aactttctat caccgccagt aatgcggcag tgcagggtgg ggaggcgatg
    31861 accacggtga tcaagacaat ggacgatatc gccgacagta cctagcgcat tggcaccatt
    31921 acttcgctga ttaacgatat tgcgtttcag accaatattc tggccctgaa tgcggcggtg
    31981 gaagcggcgc gtgccggcga acagggcaaa ggttttgcag tggtggcagg ggaagtgcgt
    32041 catttagcca gccgcagcgc taatgctgcc aacgatattc gcaagctgat tgatgccagt
    32101 gctgataagg tgcaatccgg ttcgcagcag gtacacgccg ccggacggac gatggaagat
    32161 attgtggcac aggtgaaaaa cgtcacccag ttgatcgccc agattagcca ttcaacgctg
    32221 gaacaggccg atgggctttc cagcctgacc cgtgcagtgg atgagcttaa cctgatcacc
    32281 cagaaaaatg ccgagctggt ggaagagagt gcgcaggtgt cggcgatggt gaaacaccgc
    32341 gccagccgac tggaagacgc ggtgacggtg ctgcattaat cggtatgccg gatccggcgg
    32401 ttggagcaca atgcctgatg cgatgctggc gcatcttatc aggcctgcgg ggtgtgtagc
    32461 gggtgtaggc cggataaggc gcgtcaagcg tcgcatccgg caattgcacc gcgccactgg
    32521 cggatgcggc gattaatcca gttgcgtaat atcaaaagcg gcgcggtcga tcaccgcaat
    32581 gatctttttt atttgtgcat cactgatatc gctctggttg acgcgtaaat ccagcaccgc
    32641 tttaaaatta tccagcgccc gcttcatttg cgggttctgg cgcagcgccg cgccaacgca
    32701 acgcgctttg atgcgttctt caatcatctc cacttgttcg cggttttctt ccagccactg
    32761 cgcaccttgt tcggtcagcg caatctgctt cttaccacct tcctcttcgc ggatggtaat
    32821 cagcgactgc tcctgcagaa aatccagcgt cgggtagatg acgcccgggc ttggggtgta
    32881 attcccctgg gttagattct caatcgcttt aatcaattcg taaccgtggc tgtcatcgcg
    32941 cgagagaata tccagaatca ccagacgcaa ttcaccgtga ccaaagaaac gttgccgacg
    33001 accgccgccg cgaccgtggc gaccaccgca acattgaccg tgttcatgct ggtgaccgtg
    33061 tccgcagtgc tcgtgttctg acttctcacc tttgcagcag ccctcatgcc gtggctggcc
    33121 ttcatgttta caacaccctt cgtgatgatg gctcatatca gcctcctttt gcttgattag
    33181 atatatctaa tttatatctg atgtgagcat tactgcaagt acaaaaatga tttttaaacg
    33241 tggaagcacc tcacatctag gccggataag acgttcacgc cgcatccggc agcattgcta
    33301 tggcactgaa tgccttcaca tacttcagac atatcagttc tacaaatcgc ttgcatttat
    33361 catgattaac aatcattatc atttgcgaat tttatttaga tatatctgat taccatcacg
    33421 aaggcgataa caatgaataa caccccccgc tacccgcagc gcgttcgcaa tgatctgcgc
    33481 ttccgtgaac tgactgtgct acgcgttgag cgcatcagcg ccggttttca gcgcattgtc
    33541 cttggcggcg aggcgttgga cggttttaca tcgcgcggct ttgacgatca cagcaaactc
    33601 ttctttcctc aacctgacgc tcactttgtg ccgccaacgg taacggaaga gagcatcgtc
    33661 tggccggaag gaccacgccc accgtcgcgt gactatacgc cactgtatga cgaactacgc
    33721 catgaactgg cgatcgattt ctttattcac gacggtgggg tcgccagcgg ctgggcgata
    33781 aacttacggt ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc
    33841 tgtatgtctg cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa acgttgagca
    33901 aacttgccgt taaaccgcaa gttagtgcgc tggttagcgt gcgggataac gcctgtcagg
    33961 attatctcgc gcaccttgat ggttttaata tcgaatggct ggcacatgat gagcaggcgg
    34021 tagatgcgcg tctggcgcag atgcaaatcc ctgccgatga ttacttcatc tggataaccg
    34081 gcgaaggtaa agtcgttaag aatttaagcc gccgctttga agcggaacag tatgacccac
    34141 agcgggtacg tgcagcggct tactggcacg caaagtaact gatatataaa aagaaaaagg
    34201 ctgacgattt ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg
    34261 accaagcgat tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt
    34321 ataaagtaac tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt ttataaacaa
    34381 tgtattttca atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag
    34441 gatattttgg ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc
    34501 gccgggactg gttttccctt tgctcatccg gcaaatcgct tctggaaggt gatatatcag
    34561 gccgggttta ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt
    34621 tgtggcatca ccaaactggt agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag
    34681 gagctgcacg caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaggcg
    34741 ttggcgattc tgggcaaaca agcatatgaa cagggattca gctagcgcgg tgcacagtgg
    34801 gggaaacaaa cgctcaccat tggttcgacg cagatttggg tgctgccaaa tcccagcggt
    34861 ttaagtcgcg tttcactgga gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg
    34921 gtagtgcgtg ggcgataa
//
LOCUS       DAEEDS010000038        34445 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEEDS010000038 DAEEDS010000000
VERSION     DAEEDS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.038"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..332)
                     /locus_tag="KLH32_002021"
     CDS             complement(<1..332)
                     /locus_tag="KLH32_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138853.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="KLH32_002022"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="KLH32_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBE0138854.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="KLH32_002023"
     CDS             complement(1693..2073)
                     /locus_tag="KLH32_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBE0138855.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="KLH32_002024"
     CDS             complement(2087..2503)
                     /locus_tag="KLH32_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138856.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="KLH32_002025"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="KLH32_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBE0138857.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="KLH32_002026"
     CDS             complement(3504..3971)
                     /locus_tag="KLH32_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBE0138858.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="KLH32_002027"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="KLH32_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBE0138859.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="KLH32_002028"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="KLH32_002028"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBE0138860.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="KLH32_002029"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="KLH32_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBE0138861.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="KLH32_002030"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="KLH32_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBE0138862.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="KLH32_002031"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="KLH32_002031"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBE0138863.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGLWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="KLH32_002032"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="KLH32_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBE0138864.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="KLH32_002033"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="KLH32_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBE0138865.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="KLH32_002034"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="KLH32_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBE0138866.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="KLH32_002035"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="KLH32_002035"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBE0138867.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="KLH32_002036"
     CDS             complement(14626..15441)
                     /locus_tag="KLH32_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE0138868.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="KLH32_002037"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="KLH32_002037"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBE0138869.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="KLH32_002038"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="KLH32_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBE0138870.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="KLH32_002039"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="KLH32_002039"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBE0138871.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="KLH32_002040"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="KLH32_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBE0138872.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="KLH32_002041"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="KLH32_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBE0138873.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="KLH32_002042"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="KLH32_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBE0138874.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="KLH32_002043"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="KLH32_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBE0138875.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="KLH32_002044"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="KLH32_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBE0138876.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="KLH32_002045"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="KLH32_002045"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBE0138877.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="KLH32_002046"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="KLH32_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBE0138878.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="KLH32_002047"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="KLH32_002047"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBE0138879.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="KLH32_002048"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="KLH32_002048"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBE0138880.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="KLH32_002049"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="KLH32_002049"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBE0138881.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="KLH32_002050"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="KLH32_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBE0138882.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="KLH32_002051"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="KLH32_002051"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBE0138883.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="KLH32_002052"
     CDS             31575..32189
                     /locus_tag="KLH32_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBE0138884.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="KLH32_002053"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="KLH32_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBE0138885.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="KLH32_002054"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="KLH32_002054"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBE0138886.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8103 a         8702 c         9305 g         8335 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtctg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gtgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgctctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgaacaccc
    34441 tgcgc
//
LOCUS       DAEEDS010000039        34284 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEEDS010000039 DAEEDS010000000
VERSION     DAEEDS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34284
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.039"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..138)
                     /locus_tag="KLH32_002055"
     CDS             complement(<1..138)
                     /locus_tag="KLH32_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0138887.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YT"
     gene            195..776
                     /locus_tag="KLH32_002056"
                     /pseudo
     CDS             195..776
                     /locus_tag="KLH32_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(773..1909)
                     /locus_tag="KLH32_002057"
     CDS             complement(773..1909)
                     /locus_tag="KLH32_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0138888.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1915..2238)
                     /locus_tag="KLH32_002058"
     CDS             complement(1915..2238)
                     /locus_tag="KLH32_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBE0138889.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2421..2564
                     /locus_tag="KLH32_002059"
     CDS             2421..2564
                     /locus_tag="KLH32_002059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138890.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2783..4321)
                     /gene="aldB"
                     /locus_tag="KLH32_002060"
     CDS             complement(2783..4321)
                     /gene="aldB"
                     /locus_tag="KLH32_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBE0138891.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4486..5637)
                     /gene="yiaY"
                     /locus_tag="KLH32_002061"
     CDS             complement(4486..5637)
                     /gene="yiaY"
                     /locus_tag="KLH32_002061"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBE0138892.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5827..7671)
                     /gene="selB"
                     /locus_tag="KLH32_002062"
     CDS             complement(5827..7671)
                     /gene="selB"
                     /locus_tag="KLH32_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBE0138893.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7668..9059)
                     /gene="selA"
                     /locus_tag="KLH32_002063"
     CDS             complement(7668..9059)
                     /gene="selA"
                     /locus_tag="KLH32_002063"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBE0138894.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(9157..9765)
                     /locus_tag="KLH32_002064"
     CDS             complement(9157..9765)
                     /locus_tag="KLH32_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBE0138895.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9836..10921)
                     /locus_tag="KLH32_002065"
     CDS             complement(9836..10921)
                     /locus_tag="KLH32_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0138896.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10924..11286)
                     /locus_tag="KLH32_002066"
     CDS             complement(10924..11286)
                     /locus_tag="KLH32_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBE0138897.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11823..13736
                     /gene="mtlA"
                     /locus_tag="KLH32_002067"
     CDS             11823..13736
                     /gene="mtlA"
                     /locus_tag="KLH32_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBE0138898.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13831..14979
                     /gene="mtlD"
                     /locus_tag="KLH32_002068"
     CDS             13831..14979
                     /gene="mtlD"
                     /locus_tag="KLH32_002068"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE0138899.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14979..15566
                     /gene="mtlR"
                     /locus_tag="KLH32_002069"
     CDS             14979..15566
                     /gene="mtlR"
                     /locus_tag="KLH32_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBE0138900.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15578..15787)
                     /locus_tag="KLH32_002070"
     CDS             complement(15578..15787)
                     /locus_tag="KLH32_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138901.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            16071..16433
                     /locus_tag="KLH32_002071"
     CDS             16071..16433
                     /locus_tag="KLH32_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBE0138902.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16977..17660
                     /locus_tag="KLH32_002072"
     CDS             16977..17660
                     /locus_tag="KLH32_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBE0138903.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17704..22069
                     /locus_tag="KLH32_002073"
                     /pseudo
     CDS             17704..22069
                     /locus_tag="KLH32_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22437..24092
                     /gene="lldP"
                     /locus_tag="KLH32_002074"
     CDS             22437..24092
                     /gene="lldP"
                     /locus_tag="KLH32_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBE0138904.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            24092..24868
                     /gene="lldR"
                     /locus_tag="KLH32_002075"
     CDS             24092..24868
                     /gene="lldR"
                     /locus_tag="KLH32_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBE0138905.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24865..26055
                     /gene="lldD"
                     /locus_tag="KLH32_002076"
     CDS             24865..26055
                     /gene="lldD"
                     /locus_tag="KLH32_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBE0138906.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26251..26724
                     /gene="trmL"
                     /locus_tag="KLH32_002077"
     CDS             26251..26724
                     /gene="trmL"
                     /locus_tag="KLH32_002077"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBE0138907.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26777..27598)
                     /gene="cysE"
                     /locus_tag="KLH32_002078"
     CDS             complement(26777..27598)
                     /gene="cysE"
                     /locus_tag="KLH32_002078"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBE0138908.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27678..28697)
                     /gene="gpsA"
                     /locus_tag="KLH32_002079"
     CDS             complement(27678..28697)
                     /gene="gpsA"
                     /locus_tag="KLH32_002079"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBE0138909.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28697..29164)
                     /gene="secB"
                     /locus_tag="KLH32_002080"
     CDS             complement(28697..29164)
                     /gene="secB"
                     /locus_tag="KLH32_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBE0138910.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29227..29478)
                     /gene="grxC"
                     /locus_tag="KLH32_002081"
     CDS             complement(29227..29478)
                     /gene="grxC"
                     /locus_tag="KLH32_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBE0138911.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29620..30051)
                     /locus_tag="KLH32_002082"
     CDS             complement(29620..30051)
                     /locus_tag="KLH32_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBE0138912.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30296..31840
                     /gene="gpmM"
                     /locus_tag="KLH32_002083"
                     /gene_synonym="pgmI"
     CDS             30296..31840
                     /gene="gpmM"
                     /locus_tag="KLH32_002083"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBE0138913.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31850..33133
                     /gene="envC"
                     /locus_tag="KLH32_002084"
     CDS             31850..33133
                     /gene="envC"
                     /locus_tag="KLH32_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBE0138914.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            33137..34096
                     /locus_tag="KLH32_002085"
     CDS             33137..34096
                     /locus_tag="KLH32_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBE0138915.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(34083..34284)
                     /locus_tag="KLH32_002086"
                     /pseudo
     CDS             complement(34083..34284)
                     /locus_tag="KLH32_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8528 a         8975 c         8740 g         8041 t
ORIGIN      
        1 ggtgtaagtg aactgtagtt gccatgtttt acggcaatga gagcagagat agcgctgatg
       61 tccggcagtg cttttgccgt tacgcaccac gccttcagta gcggagcagg aaggacatct
      121 gatggaaatg gaagccacgc aagcacctta aaatcaccat catacactaa atcagtaagt
      181 tggcagcatt acccaatctt gagcgtaatg ccttttatga aggcgtcgaa tttcctgatg
      241 attgtgatgt tttaatcagc tacgctccac caaaagatga atcgctggtt gccagtttta
      301 ttacacaata tgccgtaacc gcttatgcca gccagcgcta tcttgagaaa catcctatta
      361 gccgtcctga cgaactcgaa catcactcct gtattttgat cgactcgatg atgatcgacg
      421 acgcgaatat ctggcgcttc aacgtcgccg ggagtaaaga ggtgcgggat tatcgcgtga
      481 agggaaatta cgtttgcgat aacacgcaat cggcgctgga gctggcacga aatcacctgg
      541 ggattgtgtt tgcgccagac aaaagtgtgc agagcgatct gcaagacggc acgctggtgc
      601 cctgctttca gcacccgtat gaatggtggc tggatctggt ggctatcttc cgtaaacggg
      661 aatacctgcc ctggcgggtc cagtatgtgc tggatgaaat gctgcgtgaa attcgccacc
      721 agcttgctca gtcgcagcaa ctgcggcctg aacaagcggc ggaaagcgaa gattaatgat
      781 cgagataaag gtaatgcacc cagctggtca tgcgtaatag cactttgcgc atcaccgaga
      841 catggctgaa atgatcagag tagaccgcca cctgggcgta aataccgtct ggtaaagcgc
      901 tcaaatcagt gtgctcatcc agttcaatgg tcgcgataac gccatctgaa cccggcgctg
      961 tgtttaacgt ctgtaaggtg ccggtcgact gataagctcc gccgggaaca gcaggactaa
     1021 tggctgccag cttgccgctg aataccttac ctggcagagc attaaacacc acttccgcat
     1081 cgtcgccagg ggccagtcgc agcaaggagt tctgacggaa ctgcgccaca atttgtcgtt
     1141 tctgatcggg tataaacacc atcaccggac gtagcggcag cgacgcggca taggtacccg
     1201 gacgaatcag cacctgggtc acataaccat cgctcggcgc acgcactatc gtctgctcaa
     1261 ggttatattt tgcttccgcg agctgcgctt tcaggctggc gatttgagaa tgttcaccca
     1321 acaccaggct atccagctgg ctctggatct gtttttgttc cgccgccgat gactttacgg
     1381 aggcttcctg cgccagataa ttttgccgcg ccacatcaat atcgcgttct gaaaacgggt
     1441 ttactttcgc ctggctgccg cgtgcgtaac gctgatactc tttagcgaat ttatcccgcg
     1501 tggcctttgc ctgctgagta ttcgccgcca tctcatctaa ctctgcgccc aacgcccgct
     1561 gtttatgttc tgcggtaacg atatccgcca tcagccgatc cacccgcgcc tgataacgcg
     1621 tcgggtccag tcgaaatagc acctcacctt ttttaatcag cgtatttttc ttatccgtca
     1681 cttcgatcac cacgcctgtc acctgtggga caacaggaat agaaataacc gctttttgcg
     1741 ccttgaaggt atacggatgg ttatagttca ttaacagaat taaaccactg acaataaata
     1801 tgccacccag ggccgctgtg gaaatggtcc atttatttac aggaattttg aagatcttaa
     1861 acattgccca tgcaaaagcc acataggtca aaataatcag taaatccata agtattactc
     1921 ggcagaagag gatttgatgt cggcgagctg tttctccagc ccggcaatac gctgttgtag
     1981 ttgcacaacg gaggagtcat ggatttgcat tccccatccc cgctccgggc ggtaaagcgt
     2041 ggcccaaatc cacaaaaatg gccagataac atgcagcgta aaaagactca cccaaccagc
     2101 aacatgaatg gcgtcggcat gaggatgatt acgtttttta gcaatcaggt agggaatatc
     2161 atgtaaaata ataatcccat agaatatcac cagaaataca aaaataagca ctcccagtgc
     2221 aaaatagtcc aggaacatat ttccctcaaa gaatataaaa aagaacaatt aacgcatatt
     2281 atgaaatgcc atgatgcagt gcaatttcct tatttatata acgacatatt gttttttata
     2341 tactttctga aatgtgctgt ctggtttttc aacaggacga ggtttatcat attgaaatag
     2401 tggaactatt ggccaaacta atgaataact ccagtaaaac atccacagta cagattaagc
     2461 gtattaaacc ttcaattatc taccgtttat tgctgattgg cctcggatca ccaatggtga
     2521 tttacggcct ggttcgcccg ctcaccatcg aaacgcgaga ttaaactcct gacaaaacac
     2581 gccccagaaa cgcacttctg ttggcgtagt ctgggttatt gcgcacgtag gtttaaaacg
     2641 taaattgtaa caacgtgcgc tttgtttatg ccggatgcgg cgtgtacgcc ttatccggcc
     2701 tacggtcagg ttcccgtagg catgataaga cgcgtaagcg tcgcatcagg caatgaatac
     2761 ccaatgcgac cagcttctta tatcagaaca gccccaacgg tttatccgag tagctcacca
     2821 gcaggcactt ggtttgctgg taatgctcca gcatcatctt gtgggtttcg cgaccgatac
     2881 ctgattgttt gtagccacca aacgcagcat gtgccggata agcgtgataa cagttggtcc
     2941 acacgcgccc agcctgtatg ccgcgcccca tcttataggc cagattaccg ttgcggctcc
     3001 agacgcccgc gcccaggcca tattgcgtat cgttcgccag ctccagcgct tcttccatcg
     3061 ttttgaaggt ggtcaccgcc agcaccgggc caaaaatctc ctcctggaac acccgcatat
     3121 tgttctgacc aaacagaatc gtcggttcga ggtagtagcc gtctttcagt tcaccttcca
     3181 gcagcttgcg ccgcccgcct gtgagcacgt cagcgccctc ttttttaccg atatcaatgt
     3241 agttgaggat ggtttccagt tgcccgtgag aaacctgcgc gcccatttgc gtcacgctgt
     3301 cgagcgggtt accgctgcga atgctttcga cacggcggat ggcgcgttcc ataaagcgtt
     3361 cgtagattga ctcctgcact aaggcacgac tcggacaggt gcaaacttcg ccctggttaa
     3421 aggcaaacag tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct tcatccatca
     3481 catcagcaaa gaagatattt ggcgacttac cgcccaactc cagcgtcacc ggaataatgt
     3541 tttgcgttgc gtattgcata atttgttggc ccacttccgt tgagccggta aacgccactt
     3601 tggcgatgcg tttcgaggtc gccagatatt cgccaatttc cccacctgcg ccattgacca
     3661 cgttcaccac gcccggcggc agtaaatcac cgacaatttc cattagcagc agtacagaaa
     3721 gcggggtaag acgtgcgggt ttcagcacca cacagttgcc cgccgccagc gcgggagcca
     3781 ttttccagct cgccatcagc agcgggaagt tccacgggat aatctgcccc accacgccta
     3841 acggttcatg gaaatgatag gccacggttt cgctatcaac ttcactgatc ccaccttcct
     3901 gcgcccgaat acacgaggcg aaatagcgga aatggtcaat cgccagcggt acatccgcag
     3961 cactggtttc gcgaatgggt ttgccgttat cccaggtttc agctgtcgct aacagctcga
     4021 ggttttgttc cattcgatcg gcaatcttaa acagaatcgc cgcgcgatcc tgcaccgagg
     4081 tgtgcgccca tttatctttc actttgtgcg cagcatccag cgccagatcg atgtctcgtt
     4141 tgcccgaaga cgccacttcg cacagcagct gcccggtcac cggcgtcaga ttctggtaat
     4201 actcaccatc ggcaggggct acccattcac cgccaataaa gttgtcgtag cgggctttta
     4261 acttgatggg gaaaccatac tcgccgggct taatctgtgc tgaaggggga ttattggtca
     4321 tgatcgtctc cttgcggtgt gaggtataac aagggtagac gtgactggcg aaatcttcgc
     4381 cagtcggtaa cagctttacg acagctatca cgaatttacg ggcaaggatt acagagatag
     4441 ttgaaaaagc ggctgacaat ttgccagccg ttgttggaat gatgattaca ttgcggcgcg
     4501 ataaatcacc acaatttctt cgtgagttgc ctggatcggg ttagtaaagc cacaggcatc
     4561 tttcagggca ttagtcgcca gaaccgcgaa atcttcttct ttcacgttca ggtcgcgtag
     4621 gcctgccggg atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc
     4681 accttccgcg tcgtttttac ctgtcacgtt cacgcccatt gcagcggcac agtcacgcag
     4741 acgtgcagcg gcgactttgc tgttgaatac ctgaacatgc ggcagcagaa cggcgttaca
     4801 tacaccgtgt ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc
     4861 cagagaagca ttattgaacg ccataccggc aaggaactgg gcataagcca ttgcttcacg
     4921 cgctttcgca ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac
     4981 ggctttcagt gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat
     5041 agcgtgcgtt aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat
     5101 cagagaggag tcattgacag aaagcagcgg agtcacgtgc ttatcaacaa tcgccatttt
     5161 gttatgacgc gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc
     5221 cgtggtattg atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta
     5281 atcgcgaata tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg
     5341 agaaccaccg cctaaggaga tcacgctatc gcaattattt tctttaagta atttcaaacc
     5401 tgcggcgacg ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat
     5461 attgcgttct tccagtgctt tttgcacatc acccgccata cctaatttcg ttaacatatt
     5521 gtcagtgaca attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcagt
     5581 caatgaatca gcgccgatga cattcacaga aggaataaag aaagttgaag atgccataac
     5641 acactcctga aagtgtaaaa aagggtgggc agcttactga ggattttcat tcgaaaacat
     5701 gatcattatt attaattatc aaaattaatc taataaaaag cacattgtat tcattaaaca
     5761 atgtgcttta tttaaaatca taagaaattt gtattaattt attttaaaaa ttaattaatc
     5821 atttccttat ttttccggaa ataataatgc gtcgcgcaat aaatgatcat ttccacgacg
     5881 acgcgtaaag ccaatgcggt caaaatattc cagaatctga attgccagct ttcggcctac
     5941 gcctaagcga tcgcggaaat ccgccgcgca ggttgaacca cactcctgat cgagatcgcg
     6001 gatcatattg gcaaattcga caatccgatc gttacggtaa taacgatctt tgacgatcgc
     6061 ggtaattatt ccttgctgcg ccgcctggcg tagcgtcagg cgcattgcct gctcgtcggt
     6121 acccgtctct tttgccagat cacgcaccca ccacggctcg tcaccaaaca gcggctctgc
     6181 tttttgccag atggcctgct gctcttcgct gaagcccgct ttgtgatccg gcagatgcag
     6241 ccaaccgtga tgactgagga tgtcgccgct ttcgcgcatc ttttcaatca gcaacagtac
     6301 tagcgcttca tcttccattg gcaacgccat acgtcgcaga cgttcgcgcc caggcccagg
     6361 ttcatcgcga tgttgctcat gataagtcgc taatgtgtcg agaattttcc gttgccagcg
     6421 agcggcaacc ggcgcattca acaagctata accagcctga atatagccag gctgttgcag
     6481 cagttcgcgc atcccttcgc cgttgagctg gcgcgcccag gcgaaatcag gaagattaac
     6541 cgcgccgcgt tgcaggtgaa cggctaacgc ttcgctatca ttctgtgccc tggcgaggga
     6601 agccagccat tgcagatact ctggtttacg tttaccgcga cgcggcgggt taagcatcac
     6661 gacgcgcgct ccggccagcg tgttgcgggc agagatatcg cgcaatacca ggcggtcgtt
     6721 atctgccagc cataacgggg tgtcgaagac cagttcagca aggttatctt ccagcagtga
     6781 aacgcgtccc gtgacgtggc tggcggcgtg gtgaatatgc agcggctgcc actgggtcag
     6841 cggtgcatgg gtttgaagct cgacaatcac ccgtgtgaac ggctctggcg gcacatcggc
     6901 aagcagccag tcgccacggt taatctgctc tttttccgca tcacccgcga tgtcaagcgc
     6961 gatacgctgc ccggcattgg cggtttctgt tggctggttt tgcgcatgca gcgcacgtac
     7021 acgcatcggt ttatttacac cagtcagcca gagtgaatcg cctaccttca cttccccgct
     7081 taacgccgta ccggtgacga ccagcccggc accttttacg gtaaatgcgc ggtcaatcgc
     7141 gaggcggaaa ctatgttggc tggcgtgctc gcgttccggc aactgaagca gatgctcgcg
     7201 cagggcatca attccccgac cttcggttgc tgcggtgata aacagttttg cctcagcaaa
     7261 accgtattcc cgcagaacct cctttacctg gcgttcaacc tcatcaacac gcgcttcgtc
     7321 cacgcgatcg gctttggtca gcgccactgt cagcatcggg ttaccggtca gctgcaaaat
     7381 cgccagatgc tcacgggtct gtgccatcac gccgtcatcg cacgccacca ccaacagcgc
     7441 gtgatcgata ccaccaacgc ccgccagcat gttggaaaga aacttttcat gaccgggaac
     7501 gtcgataaaa ccaggcacgc gaccatccgg ctgcggccag taggcatagc cgagatcgat
     7561 ggtcatgccg cgcttttttt cttccggcag acggtcagca tttacgccag taatcgcctg
     7621 caataaggtt gttttgccgt ggtcaacgtg tccggcagtc gcaataatca tttcaacaac
     7681 atctccaaaa accgttgctc atcttcaagg cagcgtaaat ccagccacaa tcgtccgtca
     7741 taaatacgac caatcaccgg cactggcaat tcacgccagc gggcggctaa tgactcaagg
     7801 tggctaccgc gtccatcatg gggtgtaaac gttaatgccg cgctcggcag gcgatcaacc
     7861 ggcagcgaac cactgccaat ctgcgaaaga catggcataa cctgtaccgc aaactccgcg
     7921 ccgtaatgtg cgacaagggg agcctgtaaa cgttgtgcct ggatttgaat gacctctgcg
     7981 ctgcgggtaa gcaggcgcag ggtcggtaat ttttcactca gagcttcagg gtgtaaataa
     8041 agacgcaacg tggcttccag cgccgcgagg gtcattttat ccgcgcgtaa tgcacgcttc
     8101 agcgggtggc tttgcaggcg ggcgatcatc tcttttttac caacgataat cccggcctgc
     8161 ggcccgccta acaacttgtc gccggagaag ctcaccagac tgacgcccgc cgcaatcaac
     8221 tcctgcggca ttggctcttt cggcaaaccg tactggctaa gatcgaccag cgagccactg
     8281 cctaaatcag tcaccactgg aacatccagc tctttgccga gcgccaccag ttcagcttcg
     8341 tctatcgctt tggtgaaccc ctgaatgctg tagttactgg tatgtacttt catcaacagt
     8401 gcggtatttt cattcaccgc ctgacgataa tcattcgcgt gcgtgcggtt ggtggtccct
     8461 acttcgtgta gggtgcagcc tgcctgacgc ataacatcgg gaatacgaaa cgcgccgcca
     8521 atctccacca gttcgccgcg agataccacc acctctttcc cgctggcagt ggccgccaac
     8581 atcaataaca ccgccgccgc attgttattg acgatacagg catcttccgc ccccgtaata
     8641 cggcacagca gctgcgccag cgcccgatcg cgatgtccgc gtccggcgtc gtccagatcg
     8701 tactcgaggg tcactggcga acgcatagcc tgcgcaacgg cttccaccgc ggcttccgcc
     8761 tgtaaagctc gcccaaggtt ggtatgcagc acggttcccg tcaggttgat caccggacgc
     8821 agcgcgctct gcgcctcttt cgtcaaccgg gcatcgactt cttgcgccca gttttcacac
     8881 cacgcaggca gcgtctggct gccacgaatc acttctcgcg cttcgtcgag catctgacgc
     8941 aacaattcca ccacgcgggt gtgaccataa gtatcacgca aagaaaggaa ggagctatcg
     9001 cgcaataagc gatcaatagc cggaagttga ctatagaggg aacgcgtttc ggttgtcata
     9061 ggaaacctgg ctgatcaagg ccctctcaca cggagaaggg cgtttaacat aaccacggat
     9121 tgtaacgtga gatgggtcag gaggacatat cgcgcatcaa gcctttggcg gttcggtgcg
     9181 agcaaaactt tcgcggctaa acaggttttc gaccagtttg actaagtgtg ggcggtcgac
     9241 acaccaacct ggcgcaacgc ggcggaaatt gagatatccg acagcacagg caatggcgat
     9301 agtcgccaga ttgaccgtat cggttttgag tgtgccatcg accagatatc cttccagcac
     9361 atccagactg cggttgattt tctcccgctg gcgtaacaat tcatcttcag actgctgcgc
     9421 cgccggacgc gcctgttcac gcaccgatac cagcccggca tccataatgc catccgccag
     9481 cgcctcaatt ttgcgcaccc gcaacgactc cagcggatcg cgcggcaaca tcgccggagc
     9541 gacattcatt aactcaatat attcagcgat gatcggcgaa tcaaaccagc attcgccctc
     9601 ttcggtcacc agcaccggca ctttccctaa cgggttaaat tgcgccacgc cgttatccgc
     9661 gttatagggc agttcattaa taaattcgaa agttatgccc ttttctaaca acagaataga
     9721 aagtttgcgt acaaacgggc tggtgtagct accgacgagt ttcatgccga gtcctttgtg
     9781 cgaggaaaaa tatcagtatg gctcaggctc acgcaaaaca caggtgatat aagtctcaat
     9841 gatccaaata aagataatgc atccaactgg tcattcttag cagcactttc cgcatcaccg
     9901 aaacatggct gaaatggtcg gagtaaaccg ccacctgggc gtagatgccg tcgggtaagg
     9961 catcgatatc atcgttaggg tccagttcaa tggttcccag cacaccgtcc gtgccgggaa
    10021 cgaccgttaa tgattgcaat accccctgcg cctgataaga accgcctggc acgacaggta
    10081 aaatactagt caatttgccg tggaacacct gcccaggtag cgcgttaaac accacttccg
    10141 catcatcgcc aggtttcaga cgtaacagcg agttttgccg aaattgggcg acaatttgcc
    10201 gtttttgctc ggggatgaag accatcaccg gacgcagcgg caaggcagct gcgtatgtac
    10261 ctgggcggat cagtacctga gtgacgtagc cattgcttgg tgcgcgaatg acagtctgct
    10321 caagattata ttttgcttca gtaagttgcg ctcttaagct cacaatctgc gattgctcgc
    10381 cgttgaccat actgtcgagc tgactctgga tctgcgcctg ctccgccacc gagcctttca
    10441 ctaacgcatc ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt tccgagaacg
    10501 aattcaccgc cgcctggctg cctttcaggt aacgttgata atttttaaac agacggtcgc
    10561 gctccgctga aacctgggtg gtgttggcct gcgcttcagt gagctgcgca cgcagcgtct
    10621 ttatattatg cgtcgccgtc atcaggtcag cctgaagtct gtcaactcgc gcctggtaac
    10681 gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat aagctgatta ttcttgtcag
    10741 tgacttcagt aacaattccc gtcacctgtg gcgtgatagg gatcgctatc actgcctttt
    10801 gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca ctcaccagaa
    10861 acacgcctcc cagcgccgcc gtcgccagcg ccacgtaagt taaaacaatc aatagatcca
    10921 taattagatc tccgggaacg tggaatagtc agtctttttc tcggcggcga gttgatgctc
    10981 cagccgggaa attcggtcag aaagtgcggc gatttccggt tcagtcgctt tctcctgcga
    11041 cgcaacatgt gactgcatac cccagccacg ctccggttga tagagcgtcg cccagatcca
    11101 cagaaacggc cagataacat gcagagtaaa caggctcacc cagcccgccg tatgaatagc
    11161 gtcggcatgg gggtgattgc gctttttggc aatcagataa gggatgtcat gtatcgcgat
    11221 gattccataa aaaatcacca gaaagacgaa gatcagcact cccaacgcga aatagtttag
    11281 aaacatatct gcctcggatt cacgtttatc agtgttgttt tttggctggc agccagaagg
    11341 gagtcaggct gatattttga caataatccg ggttcgcgat tctcgccata acaccaaaga
    11401 ataattttta gaggtgatga gttgcttagt tacataacga ttgtatgacg aaggcataac
    11461 atgctgtaga tcacatcagg tgaacgccgt aagaaaatat cttgtgattc agatcacaaa
    11521 gattcaacaa accatcaaaa caaaaatgtg acattactca catttaaatg ccatttttag
    11581 cgaaaatcac cgccttgttt cttttttata caggcgtttt gtgatgagcg tcacgtcaat
    11641 tacctctcta ccccctatat ttatgtgact gatatcacac aaaagcccgt cgactggaca
    11701 gttaaccgat tcagtgccag atttcgcagt atctacaagg tccggctacc tctgccgcca
    11761 cattaacaaa aaacctcggg cttccagcct gcgcgacagc aaacataaga aggggtgttt
    11821 ttatgtcatc cgatattaag atcaaagtgc aaagctttgg tcgtttcctc agcaacatgg
    11881 tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta tttattccaa
    11941 cagggtggtt accgaacgag acgctggcga agctggtcgg gccgatgatc acttatctcc
    12001 taccgctgct gatcggttat accggtggta agctggtagg cggcaaacgt ggtggcgtag
    12061 tcggtgccat taccaccatg ggcgttatcg tcggcgcaga catgccgatg ttcctcggtt
    12121 ctatgattgc aggtccgctg ggcggctggt gcattaagca cttcgaccgc tgggtagacg
    12181 gtaagatcaa atccggtttt gagatgctgg tgaataactt ctccgcaggc atcatcggga
    12241 tgatcctcgc tattctggca ttcctcggca ttggcccgat tgttgaagcc ctgtccaaaa
    12301 tgctggctgc gggcgttaac ttcatggttg tccatgacat gctgccgctg gcgtctatct
    12361 ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat taaccacggt atcttctcgc
    12421 cgctgggtat tcagcagtcc catgaactgg gtaaatcaat cttcttcctg attgaagcta
    12481 acccaggtcc aggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt ggtagcgcta
    12541 aacagtctgc gggcggtgcg gcaatcatcc acttcctggg tggtatccac gaaatctact
    12601 tcccgtatgt gctgatgaat ccgcgtctga tcctcgcagt catcctcggc ggtatgactg
    12661 gcgtgttcac gctgactatc ctgggcggtg gtctggtttc tccggcatct ccgggttcta
    12721 tccttgctgt actggcgatg acaccaaaag gtgcttattt cgctaacatc gcgggtgtgt
    12781 gtgcggcgat ggctgtctcc ttcgttgtct ctgctatttt gctgaaaacc agcaaagtga
    12841 aagaagaaga tgatattgaa gcagcaactc gtcgtatgca ggacatgaaa gctgagtcta
    12901 aaggcacatc tccgctatct gcgggcgatg tgactaacga cctgagccac gtacgtaaaa
    12961 tcatcgttgc ctgtgacgcc ggtatgggtt caagtgcgat gggcgcaggc gtgctgcgta
    13021 agaaaattca ggatgcaggt ctgtcgcaga tttcggtcac taacagcgcg atcaacaacc
    13081 tgccgccaga tgtggacctc gtcatcactc accgtgacct gaccgaacgc gctatgcgcc
    13141 aggttccgca ggcacagcat atttcgctga ccaacttcct cgacagcggc ctgtacacca
    13201 gcctaaccga acgtctcgtt gctgcccagc gccatactga aaacgaagtg aaagtgaaag
    13261 acagtctgaa agacagcttt gacgattcca gtgctaacct gttcaaactg ggcgcggaga
    13321 acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt gctggcgagc
    13381 agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa
    13441 aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga
    13501 aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg
    13561 gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt aacaacgagc
    13621 acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc atcgagcgtc
    13681 tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt aagtaatcca
    13741 atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct
    13801 cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat
    13861 atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc
    13921 gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg
    13981 gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt
    14041 gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt
    14101 gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt
    14161 aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac cacgcagctg
    14221 aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agagcacgtt
    14281 ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat
    14341 ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa
    14401 ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa
    14461 cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc
    14521 ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt
    14581 gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat
    14641 gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta
    14701 gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca
    14761 ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt
    14821 gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct
    14881 gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa cagcgaggtt
    14941 gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca
    15001 ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggccg
    15061 ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca
    15121 ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag
    15181 acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg
    15241 atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg
    15301 aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc aacgagtacg
    15361 cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac
    15421 caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct
    15481 atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccaaaatca
    15541 gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg
    15601 gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg
    15661 gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct
    15721 ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt
    15781 tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggcctc caattttagt
    15841 ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc
    15901 tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc gtagtttttg
    15961 agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga acaaagcacc
    16021 tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag
    16081 tcgaaaaaaa cgaaatcaag cgtctcagcg atcgcctgga cgccatccgc caccagcagg
    16141 ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca
    16201 cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag
    16261 cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag caggctgata
    16321 tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg
    16381 ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt taagcccctc
    16441 agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt caatgcgggg
    16501 actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc gatttaccgc
    16561 tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca ataaaaatat
    16621 atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg gatttaattg
    16681 tgaaatttta atcatcatat tcacaaaatc aattatctgt gaaatttttc ccagacatgg
    16741 tttctttcat atttgacatt tattaataat catcattaga atccacgaaa aataatatat
    16801 tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa
    16861 caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct caccaataat
    16921 acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga
    16981 acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat
    17041 gtgactacat tgaagaaggc aagccagaaa gtagtttact gaaacaacaa gaagaacata
    17101 ataataaaat tgtgctactt gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta
    17161 ttcaaaaaca acaaactggc ataataaaca gtacaaagac tttaactcac gtaattaaat
    17221 ccgttaaaga ccaacaaaat acttttattt ttaccgagtt taacccagca aaaacgaagt
    17281 atttcatcct gaacaacggt tcggtggctt tagcgggtcg agtgttatcc attgacgcca
    17341 cagagaatgg tagtgttatt catatctcac tggtcaactt attaagtaca cctatctcaa
    17401 acattggttt taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc
    17461 ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc
    17521 aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga
    17581 agttagccat caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc
    17641 agaaaagaag caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca
    17701 ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc
    17761 agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg
    17821 ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac
    17881 ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc tataggcaaa
    17941 ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac
    18001 gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggtggt
    18061 gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt
    18121 gcctcttcgt attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg
    18181 ggtaaattgt ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc
    18241 ctggggcgta gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt
    18301 gccagcgcag cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact
    18361 gctattggca ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc
    18421 cttgccgata aagtcaatac tatcgcatta ggaaatagca gccaggctct ggcagataat
    18481 gcaatcgcca taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat
    18541 ggtagccagt cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt
    18601 gataagagtc ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg
    18661 ggcgcagatt ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa
    18721 cgcaagatcg ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat
    18781 ggttcacagc tttatgccat tagcgactcg gtagcaaaaa aggcttggag gaggggctgc
    18841 agtagatgtt gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa
    18901 aaataacgta ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac
    18961 caaaggcaaa tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga
    19021 tgttgcggat ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa
    19081 agctaccaat gacgatgtcg aagccaacac caccaatatc accaacctga cggattccgt
    19141 tggtgacctt caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc
    19201 tcacggccag gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga
    19261 cagcactgat gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa
    19321 taccaccaat atcgccaata acacttccaa tattgccact aacaccacca acatctctaa
    19381 cctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg
    19441 tgtattcacg gcagctcatg gcaccgagac caccagcaaa ataaccaacg ttaaagatgg
    19501 cgacctgacg actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga
    19561 tgccgtggcg acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac
    19621 tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt
    19681 cactgcagct catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt
    19741 cactgcaacc agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat
    19801 cgccacctac ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac
    19861 ctacaaaatc ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc
    19921 ctcatttagc acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag
    19981 tgccaaacac ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat
    20041 ttcagactcc agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt
    20101 tgttgatgcg ctggggggtg gtgccgaagt caatgcagac ggcaccatca ccgcaccgac
    20161 ttacaccatt gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga
    20221 taacgccctg ctgtgggata agactgccaa tggcggtgct ggagcctaca atgccagcca
    20281 tgacggtaaa gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac
    20341 cgatgcagtg aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca
    20401 aattatcaat cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg
    20461 tatcaactac gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg
    20521 tgtcggcgct acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat
    20581 tggtcagaac agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc
    20641 cagccgtgtg attgccaaag gttctcgtga cactagcgta acggaaaatg gcgtggtgat
    20701 tggttatgac accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa
    20761 ataccgccaa atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg
    20821 ccagttgcag aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa
    20881 ctcaacggca gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac
    20941 tgtcgttaat ggtaatgcag gtattggtat cggtttgaac actctggttc tggctgatgc
    21001 gatcaacggc attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat
    21061 gggtaacggt tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga
    21121 cgcaccgcag aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat
    21181 cacaaacgtc gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt
    21241 cactgatgag cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac
    21301 taatctggat actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg
    21361 tagcaccaag tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga
    21421 tagcgttgct attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac
    21481 cggttccgtt gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg
    21541 tatcaccaac gtagctgcca gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa
    21601 atcttctgaa gcaggcggag tgcgctacga caccaaagct gatggttcta tcgactatag
    21661 caatatcacc ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg
    21721 cgtcaacaac aacgacgcgg tgaactacgc gcagttgaag caaagcgcgc aggaaacgaa
    21781 gcaatacacc gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa
    21841 gttgagcggt ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc
    21901 gggtgccagc atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt
    21961 aggtgtatcg atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa
    22021 tagccagggt gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa
    22081 gttagtgtga ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat
    22141 aaggcgctcc gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta
    22201 ccagacatct ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt
    22261 tcacaattcc ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca
    22321 ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac
    22381 aaggagatga gcaacagact cattacacga tgtgcgtgga ctctaggaga cctgcaatga
    22441 atctctggca acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat
    22501 cgcttcccat cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg
    22561 ccgcctcatg gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg
    22621 tcgctaacgc gctggcctca gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt
    22681 ggatcattat tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca
    22741 tcattcgctc gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg
    22801 gtttctgttt cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta
    22861 ccgccgcatt gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga
    22921 ttgttaacac cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac
    22981 aagtaacagg catcgacagc tttgagattg gccagatggt ggggcgtcag ctaccgttta
    23041 tgaccattat cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaaag
    23101 agacgtggcc tgcggtcgtg gttgcaggcg gctcgtttgc catcgctcag taccttagct
    23161 ctaacttcat tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc
    23221 tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt ccgcttcggt gacttggggg
    23281 cgtcacaggt tgatatgacg ctggcccaca ccggttacac cgcgggtcag gtgttacgtg
    23341 cctggacacc gttcctgttc ctgaccgcta ccgtaacact gtggagcatc ccgccgttta
    23401 aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt
    23461 acctcaataa actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg
    23521 ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc
    23581 tctcgattgt ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc
    23641 tgaaagaact ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct
    23701 cgaactattc cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat
    23761 tcaccttctt ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct
    23821 catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg
    23881 atctgttgct ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc
    23941 aatctatcgc tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc
    24001 gctttactgt caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc
    24061 aggcttatgt cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga
    24121 cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa
    24181 gttgcccgct gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactacgcga
    24241 ggcgctggca aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt
    24301 tattcgctgg cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct
    24361 gatggccgat gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc
    24421 cagcaccgca tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct
    24481 ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt
    24541 tcatctggcg attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt
    24601 cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc
    24661 ggttttttca caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga
    24721 tgccgacggg gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa
    24781 acgattcgat gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa
    24841 tgagcattcg agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca
    24901 gcgcaacgca ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa
    24961 tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg
    25021 aaaaacatgt ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg
    25081 gtagcactgg caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca
    25141 gccaaagcgg cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg
    25201 attgaagaag tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc
    25261 gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg
    25321 gttttcaccg tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg
    25381 agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg
    25441 tgggatgtgg gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc
    25501 aaaccgaccg gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc
    25561 tcatggaaag accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg
    25621 atcctcgatc cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt
    25681 tctaaccacg gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct
    25741 attgcagatg cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg
    25801 cttgatgtcg tgcgtatgat tgcgctcggt gccgacacca tactgctggg tcgtgctttc
    25861 ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa
    25921 aaagagatga aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa
    25981 gattcgctgg tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa
    26041 gggaatgcgg catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg
    26101 accgttcggc gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc
    26161 ctcgcccctc cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct
    26221 gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa
    26281 attccgccaa atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctacat
    26341 atcatcgaac cgatgggatt tgcctgggac gacaagcgcc tgcgccgcgc ggggctggac
    26401 tatcacgagt ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa
    26461 aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc
    26521 tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt
    26581 cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc
    26641 atgaatctgt ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat
    26701 ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg
    26761 gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga
    26821 aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca atacgaaccg
    26881 gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa
    26941 tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca
    27001 caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca
    27061 cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg
    27121 tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga
    27181 acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca
    27241 accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg
    27301 ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag
    27361 aggcgatcat ttccgggtca gcggcgtagg cttcttcaac cacttcacgg atagcaatag
    27421 caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt
    27481 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
    27541 gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg
    27601 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta
    27661 acaaaggttc cctggggtta gtgactgctg cgctcgtcct tgcgtgcacg acctaataat
    27721 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
    27781 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
    27841 ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc
    27901 atcataccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
    27961 agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcacc aagacgcgac
    28021 atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg
    28081 tcagacatcc ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacgcca
    28141 atgaaatccg ggttgctgta aacgcggaaa cttttgccgc agtgcagcag ctgctggaga
    28201 tcatcggcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa acctgccgcc
    28261 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc
    28321 tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac
    28381 accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca
    28441 tggctgggta cgacgacgag aatattacga ctggctgcca gcgcagtggc gagatcgctt
    28501 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
    28561 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
    28621 cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
    28681 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
    28741 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
    28801 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
    28861 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
    28921 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc caaggaggcc gttacggtaa
    28981 cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa
    29041 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
    29101 agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg
    29161 acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa taccaacgtt
    29221 cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc
    29281 acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc aaccgctgcg
    29341 tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct ggaaactcac
    29401 gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata
    29461 gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag
    29521 ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat
    29581 tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg
    29641 caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc
    29701 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat
    29761 aaccgatttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg
    29821 caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    29881 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt
    29941 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    30001 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    30061 caactcccga cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa
    30121 atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa
    30181 atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    30241 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    30301 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca
    30361 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    30421 tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg
    30481 taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    30541 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc tggctggcgc
    30601 ggtacataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg
    30661 cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    30721 cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc
    30781 tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc
    30841 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    30901 agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg
    30961 tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    31021 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt
    31081 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt
    31141 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat
    31201 caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc
    31261 gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    31321 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    31381 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    31441 actggttgcg gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg
    31501 cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga
    31561 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    31621 cgctgaccac ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc
    31681 acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc
    31741 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    31801 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    31861 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    31921 ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    31981 gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgca aaagagcgcg cggtacgcca
    32041 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    32101 tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat
    32161 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    32221 cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat
    32281 tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa
    32341 ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcaccatgca
    32401 gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc
    32461 ccaacaggcg aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga
    32521 atcttccatc cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct
    32581 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg
    32641 tgaggcccag gcggttcgcg agcgccagaa agaagcgacg cgcaaaggca ccacctacaa
    32701 accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg
    32761 tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggcgagc agctacaggg
    32821 tgaattacgc tggaaaggga tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat
    32881 tgccgacggt cgagtgattc tggctgactg gctgcaaggt tacggtctgg tggtggtggt
    32941 tgagcacggt aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt
    33001 tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg
    33061 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    33121 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt
    33181 ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta
    33241 tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt
    33301 acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt
    33361 gttgattcat ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctacg
    33421 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    33481 ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc tgtttggtat
    33541 gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    33601 cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa
    33661 ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta atcgcgcaat
    33721 tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt
    33781 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    33841 cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga
    33901 tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc
    33961 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    34021 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg cgaagtttaa
    34081 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    34141 cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac gctgcgcaca
    34201 ttggtaatca ggcgcttata catacccgga agtaaatatc tcggcaatca tccgttggcg
    34261 ggtaagaata tccggctctt tgcg
//
LOCUS       DAEEDS010000040        33692 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEEDS010000040 DAEEDS010000000
VERSION     DAEEDS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33692
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.040"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            78..1355
                     /gene="waaA"
                     /locus_tag="KLH32_002087"
     CDS             78..1355
                     /gene="waaA"
                     /locus_tag="KLH32_002087"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBE0138916.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1363..1842
                     /gene="coaD"
                     /locus_tag="KLH32_002088"
     CDS             1363..1842
                     /gene="coaD"
                     /locus_tag="KLH32_002088"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBE0138917.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KLH32_002089"
     CDS             complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KLH32_002089"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBE0138918.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KLH32_002090"
     CDS             complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KLH32_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBE0138919.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KLH32_002091"
     CDS             complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KLH32_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBE0138920.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KLH32_002092"
     CDS             complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KLH32_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBE0138921.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4269..5489
                     /gene="coaBC"
                     /locus_tag="KLH32_002093"
     CDS             4269..5489
                     /gene="coaBC"
                     /locus_tag="KLH32_002093"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBE0138922.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5467..5925
                     /gene="dut"
                     /locus_tag="KLH32_002094"
     CDS             5467..5925
                     /gene="dut"
                     /locus_tag="KLH32_002094"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBE0138923.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6032..6628
                     /gene="slmA"
                     /locus_tag="KLH32_002095"
     CDS             6032..6628
                     /gene="slmA"
                     /locus_tag="KLH32_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBE0138924.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KLH32_002096"
     CDS             complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KLH32_002096"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBE0138925.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KLH32_002097"
     CDS             complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KLH32_002097"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBE0138926.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8215..9078
                     /locus_tag="KLH32_002098"
     CDS             8215..9078
                     /locus_tag="KLH32_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBE0138927.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9299..10123
                     /gene="dinD"
                     /locus_tag="KLH32_002099"
     CDS             9299..10123
                     /gene="dinD"
                     /locus_tag="KLH32_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBE0138928.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10414..11031
                     /locus_tag="KLH32_002100"
     CDS             10414..11031
                     /locus_tag="KLH32_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBE0138929.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KLH32_002101"
     CDS             complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KLH32_002101"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBE0138930.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12968..13591
                     /gene="gmk"
                     /locus_tag="KLH32_002102"
     CDS             12968..13591
                     /gene="gmk"
                     /locus_tag="KLH32_002102"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBE0138931.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13646..13921
                     /gene="rpoZ"
                     /locus_tag="KLH32_002103"
     CDS             13646..13921
                     /gene="rpoZ"
                     /locus_tag="KLH32_002103"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBE0138932.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13940..16048
                     /gene="spoT"
                     /locus_tag="KLH32_002104"
     CDS             13940..16048
                     /gene="spoT"
                     /locus_tag="KLH32_002104"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBE0138933.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16055..16744
                     /gene="trmH"
                     /locus_tag="KLH32_002105"
     CDS             16055..16744
                     /gene="trmH"
                     /locus_tag="KLH32_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBE0138934.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16750..18831
                     /gene="recG"
                     /locus_tag="KLH32_002106"
     CDS             16750..18831
                     /gene="recG"
                     /locus_tag="KLH32_002106"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBE0138935.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KLH32_002107"
     CDS             complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KLH32_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBE0138936.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20350..21741
                     /gene="xanP"
                     /locus_tag="KLH32_002108"
     CDS             20350..21741
                     /gene="xanP"
                     /locus_tag="KLH32_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBE0138937.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21862..23571
                     /locus_tag="KLH32_002109"
     CDS             21862..23571
                     /locus_tag="KLH32_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE0138938.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KLH32_002110"
     CDS             complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KLH32_002110"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBE0138939.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25952..27335)
                     /locus_tag="KLH32_002111"
                     /pseudo
     CDS             complement(25952..27335)
                     /locus_tag="KLH32_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27628..27722
                     /locus_tag="KLH32_002112"
     tRNA            27628..27722
                     /locus_tag="KLH32_002112"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27662..27664,aa:Sec,seq:tca)
     gene            28023..29207
                     /locus_tag="KLH32_002113"
     CDS             28023..29207
                     /locus_tag="KLH32_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0138940.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29619..30662
                     /locus_tag="KLH32_002114"
     CDS             29619..30662
                     /locus_tag="KLH32_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBE0138941.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31177..31605
                     /locus_tag="KLH32_002115"
                     /pseudo
     CDS             31177..31605
                     /locus_tag="KLH32_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31635..32786)
                     /locus_tag="KLH32_002116"
                     /pseudo
     CDS             complement(31635..32786)
                     /locus_tag="KLH32_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32851..33505
                     /locus_tag="KLH32_002117"
                     /pseudo
     CDS             32851..33505
                     /locus_tag="KLH32_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8436 a         8853 c         8298 g         8105 t
ORIGIN      
        1 aaaaacggga aaagtaatgg taaagccaca gctaaataca tagaatcccc agcacatcca
       61 taagtcagct atttactatg ctcgaattgc tttacaccgc ccttctctac cttattcagc
      121 cgctgatctg gatacggctc tgggtgcgcg gacgtaaggc tccggcctat cgaaaacgct
      181 ggggtgaacg ttacggtttt taccgccatc cgctaaaacc aggcggcatt atgctgcact
      241 ccgtctccgt cggtgaaact ctggcggcga tcccgttggt gcgcgcgctg cgtcatcgtt
      301 atcctgattt accgattacc gttacaacca tgacgccaac cggttcggag cgcgtacaat
      361 cggctttcgg gaaggatgtt cagcacgttt atctgccgta tgacctgccc gatgcgctca
      421 accgtttcct gaataaagtc gaccccaaac tggtgttgat tatggaaacc gaactatggc
      481 ctaacctgat tgcggcgcta cataaacgta aaattccgct ggtgatcgct aacgcgcgac
      541 tctctgcccg ctcggccgca ggttatgcca aactgggtaa attcgtccgc cgcttgctgc
      601 gtcgtattac gctgattgct gcgcaaaatg aagaagatgg tgcacgtttt gtggcgctgg
      661 gcgcaaaaaa taaccaggta accgttaccg gtagcctgaa attcgatatt tctgtaactc
      721 cgcagttggc tgctaaagcc gtgacgctac gccgccaatg ggcaccacac cgcccggtat
      781 ggattgccac cagcactcat gaaggtgaag agagcgtggt cattgccgca catcaggcat
      841 tgttacagca attcccgaat ttattgctca tcctggtacc ccgtcatcca gaacgcttcc
      901 cggatgcgat taaccttgtc cgccaggctg gactaagcta tatcacacgc tcttcagggg
      961 aagtcccctc caccagcacg caggttgtgg ttggcgatac gatgggcgag ttgatgttac
     1021 tgtacggcat tgccgatctc gcctttgttg gcggttcact ggttgaacgt ggtgggcata
     1081 atccgctgga agctgccgca cacgctattc cggtattgat ggggccgcat acttttaact
     1141 ttaaagacat ttgcgcgcgg ctggagcagg caagcgggct tattaccgtt accgatgcca
     1201 ctacgcttgc aaaagaggtt tcctctttac tcaccgacgc cgtttaccgt agtttctatg
     1261 gccgtcatgc cgttgaagta ctgtatcaaa accagggcgc gctacagcgt ctgcttcaac
     1321 tgctggaacc ttacctgcca ccgaaaacgc attgaggttg ttatgcaaaa acgggcgatt
     1381 tatccgggta ctttcgatcc cattaccaat ggtcatatcg atatcgtgac gcgcgccacg
     1441 cagatgttcg atcacgttat tctggcgatt gccgccagcc ccagtaaaaa accgatgttt
     1501 accctggaag agcgtgtggc actggcacag caggcaaccg cgcatctggg gaacgtggaa
     1561 gtggtcgggt ttagtgattt gatggcgaac ttcgcccgta atcaacacgc tacggtgctg
     1621 attcgtggcc tgcgtgcggt ggcggatttt gaatatgaaa tgcagctggc gcatatgaat
     1681 cgccacttaa tgccggaact ggaaagtgtg tttctgatgc cgtcgaaaga gtggtcgttt
     1741 atctcttcat cgttggtgaa agaggtggcg cgccatcagg gcgatgtcac ccatttcctg
     1801 ccggagaatg tccatcaggc gctgatggca aaattagcgt agcgtttatg ccggatggta
     1861 tgccatccgg ctcgcatgaa ttacttctgg cactgccgac aataaaacgt tgcccgctgc
     1921 gcatgtttag tcgccacaat cggcgtaccg cacacccgac acggctcacc ttttcgcccg
     1981 taaacctgca attcctgcgc gaaatagccc ggtttaccat cactttgcag aaaatctttc
     2041 agcgttgtac caccctgctc aatcgaacgc agcaacaccg ctttaatcac ccgagctaac
     2101 aattcacact ctgccagcga cagtgatgac gccagccgat ccggatggat ccccgccgca
     2161 aacagtgatt cgctggcata gatattccct acccctacca ccagcttgtt atccatcagc
     2221 cacggtttaa tcgccgtttt tttcttcgcg cacttctgat gcagatactc accattgaaa
     2281 tcgtcgctaa gcggctccgg tccaagatgg gccagcacat tatgcccttc cagctctttg
     2341 gtccacagcc aggcaccaaa gcggcgcgga tcggtgtagc gcagcacttt accgttgctc
     2401 atcaccaaat ccacatggtc atgcttttca gggggaagtt cttctggaag gatgcgcagg
     2461 ctgccagaca tccccaaatg aatgataatc cagccctcag gcagctccag cagcagatat
     2521 ttagcccgcc gctgcacgct aagcactggt tgatcgctta atcggtagat ctcttctgaa
     2581 accggccagc gcaagcgtcc gttgcgcacc accgcatgaa gaatggttgc accaacgaga
     2641 tgcggttcta tgccgcgacg gctggtttca acttcgggta attcaggcat agcatctcca
     2701 ggaatgaaca gatgcaatca atatgggggc aagcagataa caaaaaaccc cgccggagcg
     2761 aggtttttcg ttacatcaaa gcgagaatta tttgattttc gcttctttgt agatcacgtg
     2821 ctgacgaaca actggatcga attttttcag ttccagtttt tccggcttag tacgtttgtt
     2881 cttcgtagtg gtatagaagt gaccagtacc agcagaagaa accagcttga ttttctcacg
     2941 aataccttta gccatgattt atttcctcta agtacttagt acttttcgcc acgggcacgc
     3001 agttcagcca gaactgtatc gatgcctttt ttatcgatta cacgcatacc tttagcagat
     3061 acgcgcaggg tgacaaaacg cttctcgctc tcaacccaga aacggtgaga gtgcaggttc
     3121 ggcaggaaac ggcgtttagt cgcgttcagt gcgtgggaac ggttgttacc ggtcaccgga
     3181 cgcttgccag taacttggca gactcgggac atgtctattc tccaaaaatc aaattagctc
     3241 gagcttcgta tggggtattg gcgcctcgtc aggctttaca gcccggtcat cgcagttcta
     3301 tgtgaactct cgattgccag gcccaaatgc caaacccgag attctcaaag gtggcgtagt
     3361 atacgctgac tcagcgatgt gctcaagtcc cgaacagaca aagatcccga aggatcgcgc
     3421 atagcgggtt aaatccagcc gcgttcggca aaagaaacat actctccacg cccaatcacg
     3481 atatggtcga gcacgcgtaa atccatgaac tgacaactct ttattatccg ttcagtaatg
     3541 agtttatccg ctttactggg ttcagcacaa cccgaagggt gattatgtgc aaggatcagc
     3601 gccgaggcgt ttatttttat cgcttcgcgg ataatttccc gaggatggac ttcaacatgg
     3661 tttagcgtgc cggaaaaaag acggctatgc gttataaccc ggtgttgtga gtcgagaaag
     3721 atcaccataa agatctcccg ctcctcaccc gtgagctggc tttgtaaaaa ttcccgcgtc
     3781 atctccgggc tgagtaaagg gctttcttca cgcatccgca cgttgtagta acgtcgcgcc
     3841 agttcagcaa tcccctttaa ctgggcaaat ttcgctacgc caattccatg aacaccacta
     3901 aattgttcat attcagaggt taacaagcca taaagagagc cgaaattctc cagcatctct
     3961 tttgccaggg ttaatacatc tttaccgcgc gttccggtac gcagaaatag cgccagcagc
     4021 tcgacatccg ttaaggcgct aataccaaac ttcagcattt tttcgcgcgg catcaacagc
     4081 tgtgcattgt ttttcacctt cacctccttt gtggtgctcg catcctgtca tagccgttcc
     4141 gttaaatcga cggccagttt tcaatcctgg aaagagcctc gcaaagtgaa tctcaggtga
     4201 aggcacgaca acaaaagtat tgtgataaaa tcgccaactt ctggtgtcac ccaacaggaa
     4261 aaatcatcat gagcctggcc ggtaaaaaaa tcgttctcgg cgttagcggc ggtattgctg
     4321 cctataaaac ccctgaactg gtgcgtcgtt tgcgcgatcg cggggccgac gtccgcgtag
     4381 ccatgaccga agcggcaaaa gcctttatca ccccacttag cttgcaggcg gtttctggtt
     4441 atcccgtttc cgacagtctg cttgacccgg cagccgaagc cgctatgggc catattgagc
     4501 tgggtaaatg ggctgattta gtgattctcg cccctgccac ggcagatttg attgcccgtg
     4561 ttgctgccgg aatggcgaat gacctggtat cgacgatttg tctggctaca cctgcgcctg
     4621 tagccgtgct ccccgccatg aaccagcaga tgtaccgtgc cgctgccacg cagcataatt
     4681 tagaggtgct tgcttcccgt ggtttgctca tctgggggcc agacagtggc agtcaggctt
     4741 gtggtgatat cggtcctgga cgaatgctcg atccgttaac cattgtggat atggcggtgg
     4801 cgcatttttc gcccgtcaac gacctgaaac atctgaacat tatgattacc gccggtccga
     4861 cgcgtgaacc gctcgatccg gtgcgttata tctctaatca cagctccggc aagatgggtt
     4921 ttgctatcgc cgccgctgct gcccgtcgtg gcgcgaacgt cacgctggta tcaggtccag
     4981 tttcactacc gacgccaccg tttgttaaac gtgttgatgt gatgaccgcg ctggaaatgg
     5041 aagccgccgt gaatgcttct gtacagcagc aaaatatttt tatcggttgc gccgccgtgg
     5101 cggattatcg cgcagctacc gtggccccag aaaaaatcaa aaagcaggcc acgcagggtg
     5161 atgaattaac aataaaaatg gttaaaaacc ccgatatcgt cgcaggcgtt gccgcactaa
     5221 aagaccatcg accctacgtc gttggatttg ccgccgaaac aaataatgtg gaagaatacg
     5281 cccggcaaaa acgtatccgt aaaaaccttg atctgatctg cgcgaacgat gtttcccagc
     5341 caactcaagg atttaacagc gacaacaacg cattacacct tttctggcag gacggagata
     5401 aagtcttacc gcttgagcgc aaagagctcc ttggccaatt attactcgac gagatcgtga
     5461 cccgttatga tgaaaaaaat cgacgttaag attctggacc cgcgcgttag gaaggaattt
     5521 ccgctcccga cttatgccac ctctggctct gccggacttg acctgcgtgc ctgtctcgac
     5581 gacgccgtag aactggctcc gggtgacact acgctggttc cgaccgggct ggcgattcat
     5641 attgccgatc cttcactggc ggcaatgatg ctgccgcgct ccggattggg acataagcac
     5701 ggtatcgtgc ttggtaacct ggtaggattg atcgattccg actatcaggg ccagttgatg
     5761 atttccgtgt ggaaccgtgg tcaggacagc ttcaccattc aacctggcga acgcatcgcc
     5821 cagatgattt ttgttccggt agtacaggct gaatttaatc tggtggaaga tttcgacgcc
     5881 accgaccgcg gtgaaggcgg ctttggtcac tctggtcgtc agtaacacat acgcatccga
     5941 ataacgtcat aacatagccg caaacatttc gtttgcggtc atagcgtggg tgccgcctgg
     6001 caagtgctta ttttcagggg tattttgtaa catggcagaa aaacaaactg cgaaaaggaa
     6061 ccgtcgcgag gaaatacttc agtctctggc gctgatgctg gaatccagcg atggaagcca
     6121 acgtatcacg acggcaaaac tggccgcctc tgtcggcgtt tccgaagcgg cactgtatcg
     6181 ccacttcccc agtaagaccc gcatgttcga tagcctgatt gagtttatcg aagatagcct
     6241 gattactcgc atcaacctga ttctgaaaga tgagaaagac accacagcgc gcctgcgtct
     6301 gattgtgttg ctgcttctcg gttttggtga gcgtaatcct ggcctgaccc gcatcctcac
     6361 tggtcatgcg ctaatgtttg aacaggatcg cctgcaaggg cgcatcaacc agctgttcga
     6421 gcgtattgaa gcgcagctgc gccaggtatt gcgtgaaaag agaatgcgtg agggtgaagg
     6481 ttacaccacc gatgaaaccc tgctggcaag ccagatcctg gccttctgtg aaggtatgct
     6541 gtcacgtttt gtccgcagcg aatttaaata ccgcccgacg gatgattttg acgcccgctg
     6601 gccgctaatt gccgcgcagt tgcagtaata tgacgccgga tgacttttca tccggcgagt
     6661 ttctttaaac gccaaactct tcgcgatagg ccttaaccgc cgccagatgt tccgccattt
     6721 ccggcttctc ttccagatag gcaatcagat ctttcagggt gatgatagag atcactttgc
     6781 agttgtaatc acgctcaact tcctgaatcg ccgaaatctc gccgcgcccg cgttcctgac
     6841 gatcgagcga aatcaacacg ccagcaagcg tcgcgccatt ggcctgaata atctccatcg
     6901 actcgcgaat cgccgttccg gcggtgatca catcatctac cagcattacg cgtccttgta
     6961 acgcgctacc aaccagattg ccgccttcac cgtggtcttt tgcttctttg cggttaaagc
     7021 agtacggcag gtccaggtcg tgatgctccg ccagtgccac agcggttgtg gtggcaatcg
     7081 ggatcccttt gtaagcaggg ccaaacagca gatcgaactc aatgccggaa tccaccaacg
     7141 cttcagcgta aaaacggcct aacagtgcca gatcgcgccc ggtattaaac agcccggcgt
     7201 tgaagaaata ggggcttttg cgcccggatt tcagcgtaaa ctcgccaaac tttaacacct
     7261 gcttgctaag cgcaaattca ataaactggc gctgatatgg tttcatgcct tcgctcctca
     7321 tcttactttt ctacagacaa aaaaaaggcg actcatcagt cgccttaaaa atcagtttgc
     7381 cagcgccgcc ttctgcgtcg ctacaatgga ttcgattccc cctcgggcca gcgccaacaa
     7441 ggtgagtagc tcttcatggg tgaacggctc gccttctgcc gtcccctgca cttcaatgat
     7501 gcgcccgtct tcggtcatca ctacgttcat gtcggtctct gcggctgagt cttcaacgta
     7561 ttccagatcg caaattgctt caccgtttac aataccgaca gaaactgcgg ctaccatccc
     7621 tttcatcggg ttggttttca gcttgccgtt ttccaccagc ttctgtagcg catctgccag
     7681 cgccacgcag gcacccgtaa tcgacgcggt acgagtgcca ccatcagcct gaagcacgtc
     7741 gcagtccagc gtaatggtga actcacccag cgctttcaaa tctactgccg cgcgaagagc
     7801 acgggcgatc agacgctgga tttccattgt gcgtccaccc tgcttacctt tcgccgcttc
     7861 acgagcgtta cgggtgtggg tagaacgtgg cagcatgccg tactctgcgg tgatccagcc
     7921 ctggccctga cctttcagga agcgcggcac gccttcttca atagaggcgg tacacaatac
     7981 tttggtatcg ccaaattcga ccagcaccga gccttctgca tgttttgtat agttacgagt
     8041 cagggtaacg ggacgcacct gattattgct acggcctgct ggacgcatat tgaaatctcc
     8101 ggcttgaaac aaatgtggct gcgcattata cggacttccg gcggttattc ctatcctgac
     8161 aaggcatcga tggctataat ccttccacct ctccttttat aaacaggaac gtctatgatc
     8221 cgcagtatga ccgcctacgc ccggcgtgaa atcaagggtg aatgggggag cgcaacctgg
     8281 gaaatgcgct cggtaaacca gcgttatctg gaaacttact ttcgtctgcc ggagcagttc
     8341 cgtagccttg aacctgtcgt tcgcgagcgt attcgttctc gcctgacgcg cggtaaagtg
     8401 gaatgtaccc tgcgctatga gccagatgtt agcgcacaag gtgagctgat cctcaatgaa
     8461 aaactggcta aacagctggt aactgccgcg aactgggtaa aaatgaagag tgacgaaggg
     8521 gaaatcaacc cggttgatat tctacgctgg ccgggcgtga tggcagccca ggagcaggat
     8581 ctcgacgcga ttgccgctga aattctcgcg gcgttggatg gtacgctgga cgactttatc
     8641 gtcgcgcgcg aaaccgaagg tcaggcactg aaagcattga tcgagcagcg tctggaaggc
     8701 gtcaccgccg aagtggtcaa agtccgcgcc catatgccgg aaattctgca atggcagcgt
     8761 gagcgtctgg tcgcgaagct ggaagatgct caggttcagt tggaaaacaa ccgtctggag
     8821 caggagttgg ttctgctggc acaacgaatt gacgttgccg aagagctgga ccgcctcgaa
     8881 gcgcatgtta aagagacgta caacattctg aagaaaaaag aagcggttgg tcgtcgtctg
     8941 gactttatga tgcaggagtt caaccgtgag tcgaacactc ttgcgtcgaa atctatcaat
     9001 gccgaagtga caaactccgc catcgagctg aaagtgttga ttgagcagat gcgcgagcag
     9061 attcagaata ttgagtaact cgtaacacca attctgactt ttcagccttc acaaatgccg
     9121 cccactcaaa cagagcggca tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg
     9181 ctccagattt tgcgatgttt ttttgcccag cacactgaga acgtgagata ctcacaactg
     9241 tatataaata cagttacaga tttactttct ttgcaatcga tatcacatgg agtgggcaat
     9301 gaacgaacat catcaacctt ttgaagagat aaaactgatt aatgcaaacg gagcagaaca
     9361 atggtcagca agacaacttg ggaaactact gggttattca gagtatcgtc actttatacc
     9421 tgtattaacg cgcgccaaag aggcctgtga gaacagtggt cacacaattg atgaccattt
     9481 cgaggagatc ctcgatatgg tcaaaattgg ctcaaatgcc aaaagagcat taaaagacat
     9541 cgtactctcc cgctatgcct gttacctggt agtacaaaac ggcgaccccg cgaaaccggt
     9601 cattgcggca gggcagactt attttactat ccagacccga cggcaggagc ttgctgacga
     9661 tgaagcattt aagcaacttc gtgaagacga aaagcgtctg tttctgagaa atgagttgaa
     9721 agaacataac aaacaattgg ttgaggcagc acagcaagct ggggtagcga cagctactga
     9781 tttcgccata tttcagaatc atggttacca ggggctgtat ggtggattag atcagaaagc
     9841 catccaccaa ctgaaggggc tgaaaaagag tcagaagatc ctggatcata tgggttcaac
     9901 agaactggcg gctaatctct ttcgagctac ccaaacagaa gaaaaactca agcgggatag
     9961 cgtgaattca aaacagcaag ctaataccac acactttgac gtgggtagca aggtgaggca
    10021 aaccattcag gaacttggcg gaaccatacc tgaggagttg ccgaccccgc aggtcagcat
    10081 caagcagttg gaaaacagtg taaaaattac agagaagaaa tagcagaaac gaaaaactct
    10141 ttttgttgat tgagacaccc gatgcgtaag ccaaggtcca ggtgcagtga acacaatggc
    10201 taaacattgc acctttcttt tcccccctca gttttaacct attttttctt atgcattttc
    10261 tcagacaaga agtcagaaga atgcatctac gctacagaaa atagcgattt cacataacta
    10321 aaagttatca atttcctctc accttaaaaa atatcaatcg tgacaatgcg cacaaatcgc
    10381 taccctgcca gacagatttt tagggagaga accatgctgt tacacatttt gtatttggtt
    10441 ggcattactg ccgaagccat gacgggggcg ctggcggccg ggcgacggcg catggataca
    10501 tttggcgtaa ttattattgc aaccgccacc gcaattggcg gagggtcagt gcgcgatatt
    10561 ctgctgggcc actatccgct cggctgggtc aaacacccgg aatatgtgat tatcgtcgct
    10621 accgccgcag tactcaccac tatcgttgct cctgtaatgc cttacctgcg caaagtgttt
    10681 ctggtgctcg atgcgctcgg actggtggtc ttttctatca tcggcgcaca ggttgcactg
    10741 gatatggggc acggcccaat tattgccgtt gtcgcggcgg taaccaccgg tgtatttggc
    10801 ggcgttttgc gcgatatgtt ctgtaaacgc attccgctgg tctttcaaaa agagttgtat
    10861 gccggtgtct catttgcctc cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac
    10921 catgatgtgg tgattatctc caccctggta ttcggctttt ttgctcgttt actggcgcta
    10981 cgtcttaagc tgggattacc ggtcttttac tacagccacg aaggtcacta aggttcaaaa
    11041 cctgtgatct gctgggcagc cagccaactg cccagcttct tgatttgcgc attttccttc
    11101 cattcaataa cctgtctggc gcgcccagat ccagtccccg gcagctgctg ccagaactgc
    11161 tccgtgctaa ataaaagttg cgaccaggac cgttcatcac tggcattaag cgccgcccgg
    11221 gttagcggta ttcccattgc catcacccag cgagtaaaag gctggttacg agccagatta
    11281 aactgatgcc ataactgcgc acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc
    11341 tctggcgtta ataaaagcca ggaaaagata tgctcaaagc gatgagtctg atgcagcgcg
    11401 cgccaaccgg cctcaccaat gccatccagc cccagaacct gttttgaccc cagccagact
    11461 aagcgtgaaa tgaactgttc ctgacaaaca tcagaagcaa agtagcaggt caacgggtta
    11521 aagcggtttt ctggtggtgt cggttttgta cgttctgcac cgcgccagac cacatcatca
    11581 atgcgaggaa taccctgacc ggcaaggctg acgaggatct gatcaccagg cgcaatatcc
    11641 cactcctgcc agcgcctgac ggaaccaata ttcacccgct ggactttttt atcatccagc
    11701 atgacaggtg cgagtgacgc aaccaccgat attttaccgc tcttacccac cgcaaactga
    11761 atcgccttca cttcggcaac ctgagctaca ggttgatatt tccaggccac cagccactcc
    11821 gcctggcccg gtagccaatg acgggattcg ggttccttag cccctcgcac aaccacgcca
    11881 tcggtgacga agggtaattt cgctttccac cactcattgc gtacgcgcgc aacctcatca
    11941 gcatttttaa ccgcacgggt atacctctgc gttagagtaa aacctgcggt agccagctcc
    12001 tttaaacgat cggtcattaa ctgcggtcca tccggccatg cccagacaaa aacagccagt
    12061 gagtttagcg tgtcgctatt cccctggcgc atcatcaggc cagcaacttt tgcgcgggca
    12121 tttattcccc ccatttgttg ttggatatgc ccctcgcgct ggagaaatat ttccccctga
    12181 agcgtactgt tggctaaagg cccgctaacg gtttgcggca cagcggaaat taagctaact
    12241 ttctgcgtcc agtcctcgcc tttcaggcca ttaccgcgac tgattgcttt gttcagtttc
    12301 ccgtcccgat aaaccagggt taccgcaacg ccatcaactt ttggctgcac ccaaagatcg
    12361 ctacgttctc gcatccacag acttaatgcg tttttatccg ccattttgcg tacacccgta
    12421 tgggcaaccg gatgcataac cgcaccattt aaaggcggca tcatcacgtc gcggggctcg
    12481 ctcacaaagc agcgttgcca ctgcgtaaga cgggcgctta actgatcgta aacaccgtct
    12541 tccacctcac tttttccttc cttccagtag tcatcgtccc actgttttat ttgctgttgc
    12601 aggcgggaaa tttcttcctg tgctctggct ggcgaccagg ccggacagac cgcccacaca
    12661 gatgattgcc agcacaagat acttattaat atcgccatcc atactttcat cttcacctcc
    12721 gctgtagtca ggctggcaga tatacaacgt ggtgaaggca aagccgagtg gcaaaaacgg
    12781 agtctgcgag gacgcttcct gagaatcgtc tttattgcag taaatcacag gcaaatgcgg
    12841 aaacagctac gcaaaacgca acaactttgc gcaaaaagtg tgagcaaggg ctacgtcaca
    12901 tggccgcgcc gtgtataata agctcgtatg taggctttat ttcgctaatc acatacgaaa
    12961 gatactcatg gctcaaggca cgctttatat tgtttctgcc cccagtggcg cgggtaaatc
    13021 cagcctgatt caggctttat taaaaaccca accgttgtat gacacccagg tttctgtttc
    13081 acacaccaca cgccaaccgc gtcctggtga agtccacggt gaacattatt tctttgttaa
    13141 tcatgatgaa tttaaagaaa tgattagcag agatgcgttc ctcgaacacg cagaagtttt
    13201 tggtaattac tatggcactt cgcgtgaggc cattgagcaa gtactggcga ccggtgtcga
    13261 tgtttttctc gatatcgact ggcagggcgc gcagcaaatt cgccagaaga tgccgcacgc
    13321 gcggagtatc tttattttac cgccgtccaa aattgaactg gaccgccgtc tacgcggtcg
    13381 cggtcaggac agcgaagagg tcattgcaaa gcgtatggcg caagctgttg cagaaatgag
    13441 ccattacgcc gaatatgatt atctgattgt gaatgatgac ttcgataccg cgttgaccga
    13501 tttgaagacc attattcgcg ccgaacgtct gcgcatgagc cgccaaaagc agcgtcatga
    13561 cgctttaatc agcaaattgt tggcagactg aacctgattt cagtatcatg cccagtcatt
    13621 tcttcacctg tggagctttt taagtatggc acgcgtaact gttcaggacg ctgtagagaa
    13681 aattggtaac cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt
    13741 aggcggaaag gatccgctgg taccggaaga aaacgataaa accactgtaa tcgcgctgcg
    13801 cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac gttcgcgaac gccaggaaca
    13861 gcaagagctg gaagccgctg aattacaagc cgttaccgct attgctgaag gtcgtcgtta
    13921 atcacaaagc gggtcgccct tgtatctgtt tgaaagcctg aatcaactga ttcaaaccta
    13981 cctgccggaa gaccaaatca agcgtctgcg gcaggcgtat ctcgttgcac gtgatgctca
    14041 cgaggggcaa acacgttcaa gcggtgaacc ctatatcacg cacccggtag cggttgcctg
    14101 cattctggcc gagatgaaac tcgactatga aacgctgatg gcggcgctgc tgcatgacgt
    14161 gattgaagat actcccgcca cctaccagga tatggaacag ctttttggta aaagcgtcgc
    14221 cgagctggta gagggggtgt cgaaacttga taaactcaag ttccgcgata agaaagaggc
    14281 gcaggccgaa aactttcgca agatgattat ggcgatggtg caggatatcc gcgtcatcct
    14341 catcaaactt gccgaccgta cccacaacat gcgcacgctg ggctcacttc gcccggacaa
    14401 acgtcgccgc atcgcccgtg aaactctcga aatttacagc ccgctggcgc accgtttagg
    14461 tatccaccac attaaaaccg aactcgaaga gctgggtttt gaggcgctgt atcccaaccg
    14521 ttatcgcgta atcaaagaag tggtgaaagc cgcgcgcggc aaccgtaaag agatgatcca
    14581 aaaaatcctc tcggaaatcg aagggcgttt gcaggaagcg ggaattccct gccgcgtcag
    14641 tggtcgcgag aagcatcttt attcgattta ctgcaaaatg gtgctcaaag agcagcgttt
    14701 tcactcaatc atggacatct acgctttccg cgtgatcgtc aatgattccg acacctgtta
    14761 tcgcgtgctc gggcaaatgc atagcctgta caagccgcgt ccgggtcgcg tgaaagacta
    14821 catcgccatt ccaaaagcga acggctatca gtctttgcac acctcgatga tcggcccgca
    14881 cggtgtgccg gttgaggtcc agatccgtac cgaagatatg gatcagatgg cggagatggg
    14941 tgttgccgcg cactgggctt ataaagagca cggcgaaacc agcaccaccg cgcaaatccg
    15001 cgcccagcgc tggatgcaaa gcctgctgga gctgcaacag agcgccggta gttcgtttga
    15061 atttatcgag agcgttaaat ccgatctctt cccggatgag atttacgttt tcacaccgga
    15121 agggcgaatt gtcgagctgc ctgccggtgc aacgcccgtc gacttcgctt atgcagtgca
    15181 taccgatatc ggtcatgcct gcgtgggcgc acgcgttgac cgccagcctt acccgctgtc
    15241 gcagccgctt accagcggtc aaaccgttga aatcattacc gcaccgggcg ctcgcccgaa
    15301 tgccgcgtgg ctgaactttg tcgttagctc aaaagcgcgc gccaaaattc gtcagttgct
    15361 gaaaaacctc aagcgtgatg attctgtaag cctgggccgt cgtctgctca accatgcttt
    15421 gggtggtagc cgtaagctga atgaaatccc gcaggaaaat attcagcgcg agctggatcg
    15481 catgaagctg gcaacgcttg acgatctgct ggcagaaatc ggacttggta acgcaatgag
    15541 cgtggtggtc gcgaaaaatc tgcaacatgg ggacgcctcc attccaccgg caacccaaag
    15601 ccacggacat ctgcccatta aaggtgccga tggcgtgctg atcacctttg cgaaatgctg
    15661 ccgccctatt cctggcgacc cgattatcgc ccacgtcagc cccggtaaag gtctggtgat
    15721 ccaccatgaa tcctgccgta atatccgtgg ctaccagaaa gagccagaga agtttatggc
    15781 tgtggaatgg gataaagaga cggcgcagga gttcatcacc gaaatcaagg tggagatgtt
    15841 caatcatcag ggcgcgctgg caaacctgac ggcggcaatt aacaccacga cttcgaatat
    15901 tcaaagtttg aatacggaag agaaagatgg tcgcgtctac agcgccttta ttcgtctgac
    15961 cgctcgtgac cgtgtgcatc tggcgaatat catgcgcaaa atccgcgtga tgccagacgt
    16021 gattaaagtc acccgaaacc gaaattaatg ttttatgaac ccaacacgtt atgcacgcat
    16081 ctgcgaaatg ctcgccaggc ggcagcctga tctgaccgtc tgcatggagc aggtccacaa
    16141 acctcataac gtttctgcga ttattcgtac cgcagatgcc gttggcgtac atgaagttca
    16201 cgccgtctgg cctggtagcc gcatgcgcac catggcttcg gcagcggcgg gtagtaacag
    16261 ctgggtacag gtgaaaacac accgcaccat tggcgatgcc gtcgctcatc tcaaaggcca
    16321 gggcatgcag attctggcaa cccatctttc tgataatgct gtcgatttcc gcgaaattga
    16381 ttacactcgc ccgacctgca ttttgatggg acaggagaaa acgggcatca cgcaggaagc
    16441 attggccctg gcggatcagg acatcatcat tccgatgatc ggcatggtgc agtcgctgaa
    16501 tgtttccgtt gcctcagccc tcattcttta cgaagcccag cgtcagcggc aaaatgcagg
    16561 catgtacctg cgtgaaaaca gcatgttgcc ggaagcagag caacaacgcc tgttgtttga
    16621 aggcggctat ccggtgctgg cgaaagtcgc aaaacgcaaa ggcctgcctt atccccacgt
    16681 caatcagcaa ggcgagatcg aagctgatgc cgactggtgg gctactatgc aggctgcagg
    16741 gtaagtgcca tgaaaggtcg cctgttagat gctgtcccac tcagttccct aacgggcgtt
    16801 ggcgcagcac ttagtaacaa actggcgaaa atcaacctgc ataccgtgca ggatctactc
    16861 ttacaccttc ccctgcgcta cgaagatcgc acccatctct accccatcgg agaactactg
    16921 ccgggcgttt atgccacggt ggaaggcgaa gtgctgaact gcaatatctc cttcggcggt
    16981 cggcggatga tgacctgcca gatcagcgac ggttccggca tcctcaccat gcgctttttc
    17041 aatttcagcg cggcaatgaa aaatagcctg gcgacgggcc gccgtgtact ggcttatggc
    17101 gaagcaaagc gcggtaaata tggtgcggag atgatccacc cggaataccg cgtgcagggc
    17161 gatctcagca cgccagaatt acaggaaacg ctcacgccgg tttatccaac aacggaaggc
    17221 gtaaagcagg ccacgctgcg taaattaacc gaccaggcgc tggatctgct cgacacctgc
    17281 gccattgaag aactcctgcc gccggaactg tcacaaggaa tgatgacgct accggaagcg
    17341 ttgcgcactt tgcaccgccc gccaccgacg ctacagctta gcgatctgga aaccgggcag
    17401 catccggcgc aacgtcgtct gattctggaa gaactgctgg cgcacaacct cagcatgtta
    17461 gccttacgtg ccggagcaca gcgttttcat gcccagccgc tgagcgccaa tgacacgctg
    17521 aaaaataaac tcctcgccgc cttaccgttc aagccaacgg gcgcacaggc acgcgtagtg
    17581 gcggagatcg agcatgatat ggcgctggat gtgccgatga tgcgcctggt acagggcgat
    17641 gtaggttccg gtaaaacgct ggtcgccgcc ctcgccgcgc tgcgtgcgat tgcccacggc
    17701 aaacaggtgg cactgatggc accaaccgaa ttactcgccg agcagcacgc caataacttc
    17761 cgcaactggt ttgcaccgct cggtatcgaa gtgggctggc tcgccggtaa gcagaaaggc
    17821 aaagcgcgac tgtctcagca ggaagccatc gccagcggtc aggtgcagat gattgtcggt
    17881 acacacgcca tcttccagga acaggtgcag tttaacggcc tggcgctggt gattatcgac
    17941 gaacagcatc gttttggcgt gcatcagcgt ctggcattgt gggagaaagg ccagcagcag
    18001 ggcttccatc cgcatcagtt gatcatgacc gccacgccga tcccccgcac gctggcaatg
    18061 acggcgtatg ccgatctcga tacctcggtg atagatgagc tgccgccagg ccgcacgcca
    18121 gtgactacgg tcgctattcc tgatacccgc cgtaccgaca tcattgaccg cgtgcgccac
    18181 gcctgcataa ctgaaggtcg tcaggcgtac tgggtttgta cgttgattga agagtcagag
    18241 ttactggaag cgcaggcggc ggaagctacc tgggaagagt tgaaactggc gctaccagaa
    18301 ttgaatgttg gcctggtaca cgggcggatg aaaccagccg agaaacaggc ggtgatggcg
    18361 tcgtttaaac aaggtgagct acatctgctg gttgccacta ccgttattga agtcggtgtt
    18421 gatgtgccta acgccagcct gatgattatc gaaaaccctg agcgtctggg tctggcgcag
    18481 ttacaccagc tgcgcgggcg cgtaggtcgt ggcgcggtgg cttctcactg cgtgctgctc
    18541 tacaaaacgc cgctttctaa aacggcgcaa attcgcctgc aagtgctgcg cgacagtaac
    18601 gacggttttg tgattgcgca aaaagatctg gagattcgcg gccctggcga attgttaggc
    18661 acgcgtcaga cgggtaatgc tgaatttaaa gtggcggatt tactgcgcga tcaggcgatg
    18721 atcccggaag ttcagcgcct ggcgcgacat attcacgaac gttacccaca acaggcaaaa
    18781 gccttgatag aacgctggat gccggagaca gaacgttact cgaatgcgta aaaggcggcg
    18841 gtgagaagac cgccgcttca tcggttaacc ggcaaaaatc ggcaacatta aatacagctt
    18901 aatcaccagc gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa
    18961 cgccatgtgc gacgggccaa agcgttcagt gatcgcctgc atgttggcga ttgccgttgg
    19021 cgttgcaccg aggccaaaac cacagtgacc cgcagccagc actgccgcat cgtagttttt
    19081 gcccatcatg cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac
    19141 cagaatcgcc agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa
    19201 cgccatcgcc aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa
    19261 gacgcggtaa aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac
    19321 acagacgaag gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc
    19381 caccgtcagg cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat
    19441 gcgtcccaca tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt
    19501 cggcgtggtg gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag
    19561 caccagaccg aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg
    19621 ttcaatgaac aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga
    19681 accggccaac agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat
    19741 ggcattttgc atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc
    19801 accggcacgc aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat
    19861 taacggatcg cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac
    19921 tagtagcgcc agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt
    19981 caaaaaggag acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac
    20041 aagcgttgct aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt
    20101 catactcgtc atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt
    20161 atgctttcct gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga
    20221 aaaataccaa aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat
    20281 gtggcaaacg tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga
    20341 ttgttttcga tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgctcagact
    20401 caaaacagcg aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt
    20461 gccgcctgtc agcatctgct ggcgatgttc gttgcggtga tcactccagc gctattaatc
    20521 tgccaggcgc tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt
    20581 gcctccggtg tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg
    20641 ttgtctattc agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg
    20701 ctgaaaaccg gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg
    20761 ctggcaagtt gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt
    20821 acgccgctgg tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg
    20881 ctgacctcca ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa
    20941 aatctgctgc tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac
    21001 ccttacttac gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg
    21061 tttatgggca tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca
    21121 acgccgctct attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc
    21181 tttatgatca cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa
    21241 cagccagtgt ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc
    21301 ctgaactcgt ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac
    21361 aacggagtga tccagttgac tggtgttgcc agccgctatg tcggttttgt cgtcgcgctg
    21421 atgttgatcg tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa
    21481 ccggttctgg gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt
    21541 atcgtttctc gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg
    21601 gttggtctgg gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa
    21661 aacctgctct cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt
    21721 ttcccaccag aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca
    21781 cctctttcac cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg
    21841 catttcacgg gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc
    21901 gctctgttag tggtgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa
    21961 catatcagcg catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggat
    22021 caccgttttt ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggt
    22081 cagcccgcgc ccctggtggc aaaaagtgtc gacattgcgc taagcagtcg ccaactgacc
    22141 gaaccacgcc atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag
    22201 accgcgccgc taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc
    22261 ccgaatagcg aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca
    22321 ccagaagccg gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt
    22381 tcgctcaatg atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc
    22441 gttacgctga ctaacctggg tgccgacatc gcccgcggga cattaaccgg aaacgcgcag
    22501 cgtaacgccg acggcagctg gcaagtggaa aatctgcgca tggcggatat ccgtctacaa
    22561 agcgaaaaat cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt
    22621 ggtcgcttgg aagtcatcga tgctcgtctg caaggtccgg actgggcggt gaccgacctc
    22681 gatctcagct tgcgcaacat gaccttcagt aaagatgact ggcagacaca ggaaggcaaa
    22741 ctgtcgatga acgccagcga gttcatttat ggttcgctgc atttatttga cccgattata
    22801 aacgcggaat tttccccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg
    22861 ggtatggtca gaacgtcagg gaactggctg cgtgacggta aaacgttgat ccttgatgat
    22921 gcggcaattg ccgggctgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa
    22981 acgacacccg gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg
    23041 atcattgata tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc
    23101 aacctgacgc tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat
    23161 gccgccgccg cgacattcaa tcgtgttgat gttcgtcgcc cgtcgctggc gctgaccgcc
    23221 aacagcagca cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa
    23281 gccaccgcaa gtgtttcaca aacgccacaa cgtcagaccc atatcagcct gaatggacgc
    23341 ggtgtgccgg tgaatatttt gcaacaatgg ggatggcctg aattaccgtt gactggcgac
    23401 ggcaatattc agcttaccgc cagtggagat attcaggcca atgccccgct gaaacctacg
    23461 gttagcgggc aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat
    23521 gcgggcgtcg tttccagcgg tgaagttaca tcgacggagc cggtgcggta aacatcaatc
    23581 tcgccgggta cgactcagac gtacccggca ttccgtcaat agattacaac gtaattgtca
    23641 gcgcattccc ttgaggcgtc accaccagcc cctgctcact ttcagcctgc gaaccgcctt
    23701 gcagaccatt tactttcaca acattgcgca agcacagcgt ccagttcttc gcctcgcccg
    23761 caccagtcac agtaatcgtg ttgccagtac gtgctgcttt taaagtaaag atcaccgatc
    23821 cgtcagcagc aggcacttca cagacggctt catgcccgtc ttgcagatta aagaggtgga
    23881 atgccgtgcc ttcgtgccac acgtaatcgg gacgttgatc gttgttgccc agcgccagta
    23941 gagtgttatc acgcacataa acgggcagac tcaggaagcc gtgctgctgt ttatgccagc
    24001 gactaccgtc gagttcatcg ttgtgccaca ggtgtgtcca gcgaccttcc ggcaggtaga
    24061 actgcacatc gcccgcttca gtgaacaccg gcgcaaccat cacgttgtcg cctaacatgt
    24121 attgacggtc aaggtaatca caagccggat cgtccgggaa ctccatcatc atggcccgca
    24181 tcatcggcgt accccgcgcg ttcgcacgcg cagcttcacg atacagatac ggcatcatgc
    24241 ggcatttcag ttgcgtgaag aagcgcacca catcacagga ctcatcatcg taggcccacg
    24301 gcacacgata agatttgcta ccgtgtaaac ggctatggct ggagagcaaa ccaaacgcgc
    24361 accagcgttt gtaaacgtgc gccggagcgg tattttcaaa gccgccgata tcgtggctcc
    24421 agaagccaaa acctgaaagg ccaatagaca aaccaccgcg caggctttcc gccattgatt
    24481 cgtagttagc gtaacaatcg ccaccccagt gtaccgggaa tttctgcgca ccgacggagg
    24541 ccgagcgggc aaacaagaca gcttcttcct caccaacggt gtccttgagc acgttccaca
    24601 ccagttcgtt gtagatgtac gcataatggt tatgcatttt ctgcggatcg gaaccgtcaa
    24661 accactgaac atcaattggg atacgttcgc caaagtcggt cttaaagcaa tcaacgccca
    24721 tcgcgaccag acctttcagt ttgtcggcgt accatttgca ggcatccgga ttggtaaagt
    24781 cataaatcgc cagacctggc tgccatttat cccactgcca tagcgaaccg tccgggcgtt
    24841 tgagtaaata gcctttctct tgtaactctt taaagacggg ggatttttga ccgatatagg
    24901 ggttaatcca gacgcagatt ttcagccctt tcgctttcag gcggcggatc atcccttccg
    24961 ggtcagggaa agtcagcggg tcccactcaa aatcgcacca ctggaaggct ttcatccaga
    25021 aacagtcaaa gtggaaaaca tgcagcggca gattgcgttc cgccatacca tcgataaagc
    25081 tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt ggttagccac aggccgaagg
    25141 accacggggg cggcagcgcc ggacgaccag taaagcgggt ataacgatca agtaccgctt
    25201 tcggcgtcgg gccgtcgata acaaagtatt cgagatattc actctcaacg ctgaactgca
    25261 ctttggagac tttctccgat cccacttcaa aagagacaca ctggggatga ttgaccagta
    25321 ccccataacc acggttagtc atgtagaacg ggatattttt atacgcctgt tcagtacttg
    25381 tgccgccgtc ccggttccag gtctctaccg tctggccatt gcgcaccagg gcagtaaagc
    25441 gctctcccag accgtaaact gtttcgccaa cgccaagatc aagccgctca aacatataat
    25501 tgcgttgatt attcgtgtcc tgcacgtagc cattattttt cacctgacta ccggtaatac
    25561 gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc tttgctgaca cgcgcgctta
    25621 agttgccact tttaaactca gcataacgtt ctgtgttttc gattgtgacc ttcacgtcct
    25681 gcaaaatatt gagcggataa tgaggaccgt tattcagcgc cccctgaaaa tgctcaatcc
    25741 gcacaccgac aataccttcc tgtggggaga aaaagcgcag cgtaaataaa ggcgtatcaa
    25801 gctgccaggt acgttcacgc acatcacggg gggcagcata gaccaccatt tcattatcct
    25861 gctgttcaac ctcgaatacc tgaagcgggt gaatcaaatt gaggccaggt tgaatcaacc
    25921 agtttccatc gctaattttc attgccgttc cttagttctg cacttcttga gaggtgaatt
    25981 gttgctggca acggcgttta ccctgagcca gctgttccat aacggttttc agattgtgcg
    26041 tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag caaataacag atcgccggaa
    26101 caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct gttctgcgct ttttctgccg
    26161 catcatatcc gccataagcc agcatccagc cgataagagc gccgccgaag gccaacccca
    26221 gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt caggcgttta ccattgcacc
    26281 actcgccgta gtcgacggta tcgaacatca ttacccactg gataggtgtc accagttgat
    26341 gcaacacacc aatcacgaag atgaagacaa acatagtgat gctggcctgc atgggaacaa
    26401 agaacatcgc gaggctaatc actgccagca gggcgttcgt ccaccagaag atagtgactt
    26461 tacatttcca gtcggtcaga ggttttgcca gtgcggaacc aatcaggtta cccacgcaat
    26521 aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa aatccatgtg acgtaataca
    26581 tcatcgcccc accgcgtacg cacaccgcca ggatattgaa aatggttagt aaaccgacaa
    26641 tccgccactg gtcgttttgc cagatatcac gtaaatcttc ccgcatagac gttgttgtag
    26701 atggtgcttc aacgcgttct ttagtggtga agaaacaaaa tgccagcatc atgaatgcca
    26761 ccacggaaag gaccacgata ccgccctgga aaccgagtgg tttattatca ccgccaatta
    26821 aattaaccag tggcatcatc agaacagtag aaagcatgcc tcccgcggtc gccagcacaa
    26881 aacgccagga ttgcagcgag atacgctgag tcgggtcatt ggtgattaca ccacccaatg
    26941 cgcagtaagg gatattgacg acggtataaa gtaaggtaag ttagggtgta agtaattgct
    27001 gcatagatca ttttgccgtt catactgaga tctggcgtgc tataggccag tacacagacg
    27061 atcccgaatg gcagtgcgcc aaacagtacc cacggacgaa atttacccca gcgagagcgc
    27121 gttcgatcgg ccaacaaccc catgcaagga tcggaaatcg catccagtgc acgagcgacc
    27181 aaaaacatgg ttccgacaaa tccggcagga atgccaaaaa tatcggtata aaagaacatc
    27241 atatataaca ttacgttatc gaaaataatg tggctggcgg cgtctcccat gccataacca
    27301 attttctctt taacggacaa cacttcactc ctcatctgct tttccttgat gctatagggg
    27361 tgctgagacc gataacgcat tttgtaaacc atccctgtga gagcgaatat tccgatatct
    27421 ggttattaat ttatgattct tgttttatgt gatcgtggta gcgttaattc cgctcatata
    27481 tcattgtaaa atatgggttt tatataaatt ataatgcttt cgtgataata cgctgcgttt
    27541 attaggcgga aaaaactgat ctgggggatg tagaaactca aggaagtagc tataatgcgc
    27601 cccgcctcca tgtagcaatc gaggcgcgga agatcgtcgt ctccggtgag gcggctggac
    27661 ttcaaatcca gttggggccg ccagcggtcc cgggcaggtt cgactcctgt gatcttccgc
    27721 cagtttacct cttctgaact ctcccaatgt caataaatct cttctaatcc cttgtatatc
    27781 agtatttatt tgtatcctga ggtcaaccta cttcaccccg tatcaagttt cttttggggg
    27841 tactttaggg ggtgttattt cctattgaaa tacaagcacc cccataatag gaaaaatgtg
    27901 atacataaca acggctttta acaatatctt tgttatacaa ggggtgtatt gggggtatca
    27961 ggttagttac tggcagaaat ccccctcagg aacatgaatt catcaaaaag ccggaggatg
    28021 tcatggcatt aaccgatatc aaagtgaaaa ccgcaaaacc caaagacaaa ccctataagc
    28081 ttgctgatgg cggcggtatg tatctactga ttaacactaa tggttctaaa tactggcgca
    28141 tgaagtaccg ctttgctggt aaagaaaaga tgctttctat tggcgtatac cccgatgtaa
    28201 cgctagccga tgctcgcgag aaacgcagtg aagccaggaa actcctcgct gctggaggcg
    28261 atccaaggga ggcaaaaaag gaagaaaaaa tcgcgcagca aatgagtctg aaaaatacgt
    28321 tcgaggctat tgctcgcgaa tggcaccagt taaaagctga tcggtggtca ttacgctatc
    28381 gtgatgaaat cattgatacc tttgaaaaag acatttttcc ctacattggc aagcgcccta
    28441 ttgccgaaat caaaccaatg aagttactgg aagcactgcg taaaatggaa aaacgtggag
    28501 ctctggagaa aatgcgcaag gtccgccagc gctgtggtga ggtgttccgc tatgcaattg
    28561 ttactggccg ggcagactat aacccagccc ccgatcttgc cagtgcttta gctacaccga
    28621 aaaaagtaca ttttcccttt cttactgcta atgaacttcc ccacttcctc aacgatctgg
    28681 cgggttatac cggaagtata atcactaaga cagctactca gatcattatg ttaaccggcg
    28741 tacgaacaca agaactgcgt tttgcacgct gggaagatat cgattttgag acaaaattgt
    28801 gggagatccc ggcagaagta atgaagatga agcgacctca tattgtgccg ctctctgagc
    28861 aggtcattat gctatttaag cagcttgaac caatctcaaa acatcatccc ctagtcttta
    28921 tcggtagaaa tgatccccgc aaaccaatta gtaaggaaag cattaaccaa gtaattgaat
    28981 tacttggata taagggaaga ctcacagggc acggtttcag acacaccatg agtacaattc
    29041 tgcatgaaca aggctttaat tccgcctgga ttgaaatgca gttagctcat gtggataaaa
    29101 attccatcag gggtacctat aatcatgccc tgtatctcga tggccgccgt gaaatgatgc
    29161 aatggtacgc tgattacatt gattcactct ccagccggga aagttaagtc agaaatactt
    29221 ttccctgatg ttttcctcaa ttaagtattt tatcctctgg cctacaaggc tggaggattt
    29281 ttgttattat attatttttt tgcgtcttca aacttattac aaaaacataa ttaaaaacaa
    29341 attgacattt ttaaattggc aattacaaaa tacagccttg cagttatttc gataccttta
    29401 gcaaaggaat agttgattca gatataattt agcatttcaa tcatctgaca tcattataaa
    29461 aaatgagatt cacataaaga taaaattatt gttctcaagg aaagcatgaa ggggcttcgt
    29521 aagcaatatt cattcggtgc caagacagga cttgatacac agaatctcaa cttttatgct
    29581 ctcagactat atcaccacat gttttacagg ctcagaaaat gaacgataga ttatgctttg
    29641 aagtgcacga taacaagggt tatttcgtat ttccggatac ctggtttggc ccattgctgg
    29701 gggagtttga agaagtgctt gatgcatacg acgctgatga aatcagcgag acgagttaca
    29761 tcaacaaact ccgacgcctt gctcaacggg agccggactt tattgatata cacgctcatc
    29821 tggcatatgc atttctggaa cagaatgccc cgcgtaaggc actgaacgcc gctcttaaag
    29881 gactggcggc aggtaatcgt atcatcccgg aaagtttctg tggtgaaata atctggatgc
    29941 atcccgaaaa ccggccttat ctgcgcgcgc tttatgcagc aattctcgca aatgtgcact
    30001 tacagcgcca tcaggatgcg gttatgctca ctgataaaat actggcatac aatcctgaaa
    30061 ataatcaggg ggctcgctgg ttgttgggtt cagagctgtt acgcaccggc gatcatgagc
    30121 gagccttcag tgtactgaaa gagcatgctg atgaattttc accttactgg tatgaactcg
    30181 gtctcctgca ttttctgaac ggggaacatg ttaaggccgc cacagctttt cgccatggct
    30241 ttgccacaaa cacttacatc gcagaaatgc tgtgcggaaa tctgcatcca tttccacttg
    30301 ctgtgtggca tgacttctcc ggctctctgg atacggcaga agattactat gcaacatact
    30361 caccattatg gggacaatat ccggaagcat tgctgtttgt aaactggctc tataaccatt
    30421 cctccgtgct gcatgaacga tcagaaatca ttaaatgcgc cgaaatgctg attcaggaag
    30481 atgatttcga aatatgcgaa agtattttaa gacagcagga gcacctgtgg aaaagaattg
    30541 acaagacact ttctgaggaa attgtacaga aatgcagaaa tatgaacggt gaatatatct
    30601 ggccctggat attgcccttt tccgcagcag ggatgaaaca ttcaagcatc cagtatcagt
    30661 agatattgca ttagtgtaac tttaaaacaa gtaataattt attatccaat aataacactg
    30721 tgttccttcc ccaggtgatt accctggggt tattttctga aagtttcctt tgcaaaagga
    30781 tagcgttgta aattacttta acgaattact ccatgaactc attaaagttc tttcgtaata
    30841 ctttatcgtc aaacacgtta tagctggcat caaaaaccac agatttaccc tgccagcggg
    30901 taatcccctc ctctggcgtt ttcggttgaa ctggtatccc cgtccaacac aggcagccat
    30961 tataaacagt aaaaaattgt attcgctgtt atgaaatcaa tagtcctgac gtactgttga
    31021 atgtgcttgt gttctgaacc tcgtacaacc agtacatttg atatcgaatt ataaacaaaa
    31081 cattgcatcc aactttttta atatggacaa tagttgtcca tacggtggtt ttatattcga
    31141 taaatcaatg gatttcattg agtcacagga ctggtaatgg atgaaaatgc tttaaggttt
    31201 gcctcatact ggcgcaactc gcttgcagat gctgagtcag gaaagggctg ttttgaacgg
    31261 aaagacgccc aaaatttcac tcactggcat gggatagcgg cgggacgtct tgacgaagcg
    31321 attgtcaata aattttttaa gggagaaaaa gacgatgtcg aaactgtcaa tgtcatctta
    31381 cgcccaaaag tttatttccg gttactgcag catggtaagg accgttccgc aggcacgcct
    31441 gatattgtta caccgatagt gacgccagcc ttgctaagcc gtgagggttt tttatatccg
    31501 acgccagcga cctccattcc cagagacctg cttgaacctt tgccaaaagg agcattttcg
    31561 attggtgaga ttgggcagta tgacaaatac aagacgaccc atactgatcc tacccacgta
    31621 atgtggacac ggccctaagc gagattatgg ttttcaaatt gttccgggct gagaccgcca
    31681 caggcactgt gacgacgcca gcgattgtaa tcacactcga tataattaaa caccgttgcc
    31741 cgcattattt cccggctgct aaagcgttcc ccgtggatac attccacctt cagcgaatga
    31801 aagaagcttt ccacacaggc attgtcataa cagtagcctt tcgcactcat actgccacgc
    31861 aggttgtgtc gcttcagcat tgcctgataa tcccctgaac agtattgacc accacggtcc
    31921 gtatgaacaa tgacgctttc cgggcgtctt ctccgccaca acgccatttg cagtgcatca
    31981 caggccagtt gtgctgtcat tcgcggtgac atcgaccagc caatgacgga gcgtgaccac
    32041 aggtcgatga ctactgcgag atacaaccag ccctcatcgg tacgcaagta cgtgatgtca
    32101 cccgcccact tctggtttcg ggccactggc gctgaagtcc tgctccagca gattctccaa
    32161 tacgggcagg ccatgtgcac ggtagctgac cgggctgaac ttccggctgg ctttcgcccg
    32221 cagcccctga cgacgcaggc tggcggcaat ggttttaata ttgaactccg gcagttcgtc
    32281 agcaaggcgg ggagcaccgt atcgctgctt tgcctcaaag aatgccttat ggacagcggc
    32341 atcgcaggtg agccgaaact gttggcgcag gctcatctgg tgacgacgcc tgagccagac
    32401 ataccagccg ctgcgggcaa ctcgaagtac acgacacatc gctttgatgc tgaactctgc
    32461 ccgatgattt tcgataaaga catacttcat ttcaggcgct tcgcgaagta tgtcgcggcc
    32521 ttttggagga tggacagttc ctcagcctgc tccgccagtt gtcgtttaag gcggacattt
    32581 tcagcggcca gttcgctttc gcgctctgac gaactcattt gttgctgctg tttactgcgc
    32641 caggcataaa gctgagattc atacaggctg agttcacggg ctgcggcggc cacaccgatg
    32701 cgttcagcga gtttcagggc ttcgttacga aattcaggcg tatgttgttt acggggcttc
    32761 ttgctgattg atactggttt tgtcatgagt cacctctggt tgagagttta ctcacttagt
    32821 cctgtgtcca ctattggtgg gtaagatcac cctccattaa aactaaaacg ggcgctttct
    32881 gatatatcat ctgtaagcgc agtaatacca ttagtagcac ctctcggcgg cgcattgatt
    32941 attgaatatt ataactggaa tgttatattt cttgttatga tgctgttcag tattataata
    33001 atgttattat caccaaagat aactcatgca aatgagaaag taatatgtca aaacagtgca
    33061 tcctttgtga ccaaagagtt tatctgcggc actatacttg tctcattaat actcagttct
    33121 ctattttgct acatatcaat atcaccatca attttcatgc atacttatgg cctgagtact
    33181 tttagttact caattatttt ctccttctca gttttatttt tcataatcgg aaatcaatta
    33241 tcaaagattg aaaaattatc caacctatgg tttttattac ccaatagcca attttatcgc
    33301 attacaaata aatggttcaa aaacaggatt tgccgcatct attacaggtt ttactcaaac
    33361 aataagcgct ggtacaatca gtttcctgtc agtaaccgta agcgacaacg gtgttggtcc
    33421 cggaactact ttaggtcttg gttcactaat aacttcatta ctgtcaatag ccgtaatcgc
    33481 cattgcagga aataataaaa agtaagtgta ctctgactgc gtatgtatct acttgaaccg
    33541 ccccggtttt cctggagagt gttttatctg tgaactcagg ctgccagatc atcgtttccg
    33601 atggaagcat aataagcttt ttctgcttct gccggaggag tatggcccag cctttccagc
    33661 aatcgtctat tgttatacca gtccacccac gt
//
LOCUS       DAEEDS010000041        32633 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEEDS010000041 DAEEDS010000000
VERSION     DAEEDS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32633
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.041"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..956)
                     /locus_tag="KLH32_002118"
     CDS             complement(<1..956)
                     /locus_tag="KLH32_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBE0138942.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="KLH32_002119"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="KLH32_002119"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBE0138943.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KLH32_002120"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KLH32_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KLH32_002121"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KLH32_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBE0138944.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="KLH32_002122"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="KLH32_002122"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBE0138945.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="KLH32_002123"
     CDS             complement(5018..6346)
                     /locus_tag="KLH32_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBE0138946.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="KLH32_002124"
     CDS             complement(6354..8702)
                     /locus_tag="KLH32_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0138947.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KLH32_002125"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KLH32_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBE0138948.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KLH32_002126"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KLH32_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBE0138949.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KLH32_002127"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KLH32_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBE0138950.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KLH32_002128"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KLH32_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBE0138951.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KLH32_002129"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KLH32_002129"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE0138952.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="KLH32_002130"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="KLH32_002130"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBE0138953.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="KLH32_002131"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="KLH32_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBE0138954.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KLH32_002132"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KLH32_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBE0138955.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19171
                     /locus_tag="KLH32_002133"
                     /pseudo
     CDS             18273..19171
                     /locus_tag="KLH32_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
     gene            19177..21609
                     /locus_tag="KLH32_002134"
     CDS             19177..21609
                     /locus_tag="KLH32_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBE0138956.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21755..22570
                     /gene="ybiV"
                     /locus_tag="KLH32_002135"
     CDS             21755..22570
                     /gene="ybiV"
                     /locus_tag="KLH32_002135"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBE0138957.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22722..23987
                     /locus_tag="KLH32_002136"
     CDS             22722..23987
                     /locus_tag="KLH32_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBE0138958.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24228..25820)
                     /locus_tag="KLH32_002137"
     CDS             complement(24228..25820)
                     /locus_tag="KLH32_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBE0138959.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26039..26959
                     /gene="ldtB"
                     /locus_tag="KLH32_002138"
     CDS             26039..26959
                     /gene="ldtB"
                     /locus_tag="KLH32_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0138960.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27017..28135)
                     /locus_tag="KLH32_002139"
     CDS             complement(27017..28135)
                     /locus_tag="KLH32_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBE0138961.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28132..28599)
                     /gene="mntR"
                     /locus_tag="KLH32_002140"
     CDS             complement(28132..28599)
                     /gene="mntR"
                     /locus_tag="KLH32_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBE0138962.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28785..28913
                     /gene="mntS"
                     /locus_tag="KLH32_002141"
     CDS             28785..28913
                     /gene="mntS"
                     /locus_tag="KLH32_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBE0138963.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29185..30768
                     /gene="opgE"
                     /locus_tag="KLH32_002142"
     CDS             29185..30768
                     /gene="opgE"
                     /locus_tag="KLH32_002142"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBE0138964.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30816..31331)
                     /gene="ompX"
                     /locus_tag="KLH32_002143"
     CDS             complement(30816..31331)
                     /gene="ompX"
                     /locus_tag="KLH32_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBE0138965.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31384..31449)
                     /locus_tag="KLH32_002144"
     CDS             complement(31384..31449)
                     /locus_tag="KLH32_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138966.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31684..32571
                     /gene="rhtA"
                     /locus_tag="KLH32_002145"
     CDS             31684..32571
                     /gene="rhtA"
                     /locus_tag="KLH32_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBE0138967.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8169 a         8699 c         7882 g         7883 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatac catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 cccggaaaaa ccgcttgatg cgccagagct gctcgatttt gcccagcagt atgcctgcca
    19141 gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa actggacacg
    19201 ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa gccgccagtc
    19261 tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga taagccgatc
    19321 ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat ctggatcaaa
    19381 cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc gatcttcccg
    19441 gaatatactg tctcgtggat cgaaaaagag attgatgatc tggcagatcg tcccggtgct
    19501 ggctttgcgg tgagcgaaga gaacaaacgt gttctgcatg aagtgtgccc atggtggcgc
    19561 ggtcagacgg tacaggatcg ctgctatggc atgtttaccg atgagcaaaa aggtctgctg
    19621 gcgaccggca tcattaaagc ggaaggcaat atgacctccg gcgatgcgca cctggcggtc
    19681 aatttcccgc tgctgctgga aaaagggctt gatggtctgc gtgagaaagt agcggaacgt
    19741 cgctcgcgca tcaatctgac ggtgctggaa gatttgcacg gcgaacagtt cctgaaagcg
    19801 attgatatcg tgctggtggc agtcagtgaa cacattgaac gtttcgctgc cctggcgcgt
    19861 gcaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg aactgctggc gatggcagaa
    19921 aactgcgatc ttatcgccca ccagccgccg cagactttct ggcaggcgct gcaactgtgt
    19981 tacttcatcc agttgatttt gcagattgaa tctaacggtc actcggtgtc gtttggtcgt
    20041 atggatcagt atctctaccc gtactatcgc cgcgacgttg aactgaacca gacgctggat
    20101 cgcgaacacg ccatcgaatt gctgcatagc tgctggttga aattgctgga agtgaacaaa
    20161 atccgctccg gctcccactc aaaagcctct gcgggaagtc cgctgtatca gaacgtcacc
    20221 attggcggac aaaatctggt tgatggtcaa ccaatggacg cggtgaatcc actctcttac
    20281 gcgatcctcg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac
    20341 catgcaggaa tgagtaacga tttcctcgac gcctgcgtac atgtgatccg ctgcggcttc
    20401 gggatgccgg cattcaacaa cgacgaaatc gtgatcccgg aatttattaa actcggtatt
    20461 gaaccgcagg acgcttatga ctacgcagcg attggttgta tagaaaccgc cgtcggtggc
    20521 aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt gatgctggcg
    20581 gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg
    20641 ttgtcggcag gtaacttcaa caacttcgat gaagtgatgg acgcgtggga tacgcaaatc
    20701 cgttactaca cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat gctggaagag
    20761 aacgtgcacg atattctctg ctcggcgctg gtggatgact gtattgagcg agcgaaaagt
    20821 atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggtcgg tattgccaac
    20881 ctcggcaaca gcctggcggc agtgaagaaa ctggtgtttg aacagggcgc gattggtcag
    20941 caacagctgg ctgccgcact ggcggatgac ttcgatggcc tgactcatga gcaactgcgt
    21001 cagcggctga ttaacggtgc gccgaagtac ggcaacgacg atgataccgt cgatacgctg
    21061 ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa cccacgctac
    21121 ggtcgtggtc cggttggcgg caactattac gcgggtacgt cgtctatctc cgctaacgta
    21181 ccgtttggcg cgcaaactat ggcaacgccg gacgggcgta aagcccacac cccgctggca
    21241 gaaggcgcta gtccggcctc tggtactgac catctcggcc ctactgcggt cattggctca
    21301 gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt tgctcaacca gaaactgaat
    21361 ccggcaacgc tggagaacga atctgacaag cagaaactga tgatcctgct gcgtaccttc
    21421 tttgaagtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga aacgctgctg
    21481 gaggcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc gggttattcc
    21541 gccttcttca ccgcgctctc tccagacgct caggacgata tcatcgcccg tactgaacat
    21601 atgctgtaat ctcttcggcc cggctgcggc gtcgccgggc taaatcattc acatcatcaa
    21661 tttcatcctt actttcattc gaaatataat ttgtgctctg cgtcacattg ttattacact
    21721 gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta tcgtcacaga
    21781 tatggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt ttatggcgca
    21841 atatcaggaa ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta atcagtatta
    21901 ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg
    21961 cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc gacatgaatc
    22021 gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg cctgcggtct
    22081 gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga tggcaaaaca
    22141 ctaccatcgc ctgaaacctg taaaagatta tcaggagatt gacgacgtac tgttcaagtt
    22201 ttcgctcaac ctgccggatg aacaaatccc gttagtgatc gacaaactac acatagcgct
    22261 ggatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacctga ttattcccgg
    22321 tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa
    22381 tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc
    22441 ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc gcccgttacg ctaccgatga
    22501 taataatcat gaaggcgcgc tgaatgtgat tcaggctgtg ctggataaca catccccttt
    22561 taacagctga cctctctacc ggagccatgc gctccggttt tcccttcttt ttacatcctg
    22621 tacatcacat caaatcatta aacttgcatt aacttattta taacttgtat tgtcacatgc
    22681 aaattagcat agtttcgaat gaaagttaat gcgaggtggt tatggcttct acttttacca
    22741 gcgacacatt gcctgccgat cacaaagcag ctatccgtca gatgaagcac gcgctgcggg
    22801 cgcagcttgg cgatgtccag cagatcttta atcagctaag cgatgacatt gccacgcgag
    22861 tggctgaaat caacgcactc aaagcacagg gcgatgccgt ctggccggtg ctgtcttatg
    22921 ccgatatcaa agcaggtcat gttactgcag agcagcgcga acagattaaa cgtcgcggtt
    22981 gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct aggctgggat cagtcgatgc
    23041 tggactatct gaaccgcaac cgctttgacg aggtctacaa aggccccggc gataatttct
    23101 tcgggacgct cagcgcttca cgtcccgaga tttaccccat ctactggtcg caggcgcaaa
    23161 tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat cgtctgtgga
    23221 catttgaaag tgatggaaag caatggttta acccggatgt gagcgtcatc taccctgacc
    23281 gtatccgccg ccttccgccc ggaacgacct ccaaaggtct tggagcgcat accgactccg
    23341 gggcgctgga acgctggctg cttccagcgt atcagcacgt tttcgccaac gtctttaatg
    23401 gcaatctggc gaaatacgat ccctggcatg cggcacatcg tacggaagtt gaagagtaca
    23461 cggtggacaa caccaccaaa tgttccgtgt ttcggacatt ccagggctgg actgcgctct
    23521 cggatatgct gcctggtcag gggttgctgc acgtcgtgcc cattcctgaa gccatggcgt
    23581 acgtactgtt acgtccgctg cttgatgatg tgccggagga tgaactgtgc ggcgtagcgc
    23641 ccggaagagt gttgccggta tcagagcaat ggcatccact gttaattgag gcgttaacca
    23701 gcattccaaa actcgaggcc ggagactccg tctggtggca ctgcgacgtc atccattccg
    23761 ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat gtacattcct gcggcaccga
    23821 tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa aaaggcgcat
    23881 cgccgggcga cttcccgcgc gaggactatg aaacaaactg ggaaggacgc tttacgcttg
    23941 ccgacctcaa cattcacggt aagcgagcgt tgggcatgga tgtttgagag ttaatggaag
    24001 tgaattgatg tgggattaag aaaatcgatt cgacgttact cagattgctg acaagtgcgc
    24061 tttcttaatg cctgatgcgg cgttaacgcc ttatccggcc tacaaagtcc tgcagattca
    24121 atatattaca ggtactgcgt aggcctgata aacgtagcgc atcaggctat tttgcatttg
    24181 tcatcagccg tcgtcacccc gacagtatcg gggtgacgga taatcactta ctcgatccct
    24241 ttactacgca ggtaatcttc gtaattaccg ctaaagtcga tcacgcgttc cggggtgatt
    24301 tccagaatgc gggtcgccag ggagcttacg aactcacggt cgtgtgaaac aaagatcagc
    24361 gtgccctgat acagttccag cgccatgttc agtgactcga tggattccat atccaggtgg
    24421 ttggtcggtt cgtccatgat cagaatgttc ggcttctgca tcattaactt accaaacagc
    24481 atccgccctt tttccccacc ggaaagcact ttagctggct ttttgatgtc gtcctggctg
    24541 aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt catcgccttc ctgcttccac
    24601 tgactcatcc attcgaacac ggtcagatca ttttcaaact catattcgtg gtcctgggcg
    24661 tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc cgctgtccgg ttgcagatcg
    24721 cccaccagcg ttttcagcag cgttgattta ccgacgccgt tggtacccag taccgcaagt
    24781 ttttcaccga cctccagcag caggttgaga tttttaaaca gtggaccgtt atcaaaccct
    24841 ttggtcagac cttccacttc cagcgcgtta cggaacagtt tcttatcctg ttcaaaacgg
    24901 ataaacgggt tctgacggct ggaggctttc acctcttcca gtttgatttt atcaatctgg
    24961 cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg cgctaaagcg gctaacgaaa
    25021 gattgcaact cagcaatctg cgctttcttc ttggcgttat cggccagcag acgttcacgc
    25081 gcctgggtcg ccgccgtcat gtactcatcg tagttacccg gataaacgcg cagctcgccg
    25141 taatccagat ccgccatgtg ggtacagacc atgttaagga agtgacggtc gtgcgagatg
    25201 atgatcatgg tgctgtcacg ctcgttcagc acctgttcca gccagcgaat ggtgtcgatg
    25261 tcgaggttgt tggtcggttc gtcgagcagg agaatatccg gctcagcaaa cagcgcctgc
    25321 gccagaagca cacgcagctt ccagccagga gcaacttcac tcatcgggcc gtagtgctgt
    25381 tccactggaa ttcccacgcc aagcaacagt tcaccggcgc gagcttccgc agagtaaccg
    25441 tccatttcgc cgtatttcac ttccagatcg gccactttat agccgtcttc ttcactcatt
    25501 tccggcaaag catagatgcg gtcgcgctcc tgcttcactt cccacaactc tttatgcccc
    25561 atgatcaccg tatccagcac agtgaactct tcaaaggcaa actgatcctg acgcagctta
    25621 ccaatgcgct cgttgggatc gagggaaacg ttacccagcg tcggctcaag gtcgccgccg
    25681 agaatcttca taaaggtgga tttaccacta ccgttcgcgc caatcaggcc gtaacggttg
    25741 ccgccgccga atttgacgga aatgttttca aacaacggct tactgccgaa ctgcatggtg
    25801 acgttactgg aaactaacac aggcgtatcc tgaaaagaga tatgacaaac cgcgcattat
    25861 gccataagtc tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa gccaaaggca
    25921 aaaaatgtga tttcgtacac atctgatttc actgtgagct ggaatgaact tataatgcgc
    25981 ttccaatact ctaatattct caacccaatg gcctgccagg cacaaaatct cgcttaacat
    26041 gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct gttgtcggct tttgcagtac
    26101 cgcctctgcg gtaacttatc ctctgccaac cgacgggagt cgcctggttg gtcagaatca
    26161 ggtgatcacc attcctgaag gtaacactca gccgctggag tattttgccg cggagtacca
    26221 gatggggctt tccaatatga tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg
    26281 cggtactgta ctgaacattc cgcagcagct gatcctgccg gataccgttc atgaaggcat
    26341 cgtcattaac agtgcagaga tgcgtctgta ttactatccg aaagggacca acaccgttat
    26401 cgtgctgccg atcggcattg gtcagttagg caaagatacg cctatcaact ggaccaccaa
    26461 agttgagcat aagaaagcag gcccgacctg gacgccgacc gccaaaatgc acgcagagta
    26521 ccgcgctgcg ggcgaaccgc taccggctgt cgttccggca ggtccggata acccgatggg
    26581 tctgtatgca ctctacatcg gtcgcctgta tgctatccat ggcaccaacg ccaacttcgg
    26641 tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct
    26701 gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt gatgagccgg taaaagcgac
    26761 caccgagcca gacggcagcc gttatattga agtccataac ccgctgtcta ccaccgaagc
    26821 ccagtttgaa ggtcaggaaa tcgtgccaat taccctgacg aagagcgtgc agacagtgac
    26881 cggtcagcca gatgttgacc aggttgttct tgatgaagcg atcaaaaatc gttccgggat
    26941 gccggttcgc ctgaattaat cgtcaaaacg taaaacaaag gcggactcat aatccgcctt
    27001 ttttatttgc cagaccttac caggccggga tcataactaa caaaacgtat cccaccagcg
    27061 ccgcccacaa cagcatcggt attgaataga gatggaaacg ccaccagatg cggcgatcgt
    27121 tcgccatacg tagcgcaatc aaatttgcca gcgatcctgg taataaacca aagcccccca
    27181 cgtttaccgc ccatgccagt aataaagacg gaggtacata attgagcagc aaaatggtac
    27241 tcggcacgtt actgatcacc tgcgataaac cgactgccgt taaccataac ccgggttcag
    27301 ataaatgact cacgttaccc aacacgcctt gcaacgctgg aagctgggtc agtaaatgga
    27361 cgtcgataaa catcgccata aacaccagca gcaacgtcca gtccacactc aacaccacgc
    27421 gacgcgccag cagcacaaag cccgccgcca caatcaccag tccccacagc tcttgtttga
    27481 actccagcgc cgtcagaaag acgatataca gccccagaca attccacacc agccgcggtt
    27541 tccactccgg tgtttgcacc cccgtatggt attgcagcgc ctttccaggg aaacaacacc
    27601 agcacaacag caggagtgtc agcatcattg cgccagccat cggtgccatt tgggcaataa
    27661 atccggcaaa cgaaagacca gaacgtcccc agataagaat attttgcggg ttgccaattg
    27721 gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa aataatcagc cgattaaccg
    27781 ggatctcaca cagtcttttt agcgtgatag tcagcggaac aacaataaac agcgcgacat
    27841 cgttggtcag aaaggtagaa agcagcgccg ccgccagcac cataaacatc gccagccgac
    27901 gctccgtagc aaagcggcgc accattttgc gccccagcac atcaaaataa ccgcttaact
    27961 ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag
    28021 cagcaggcca ggatttcggt gcaaagggca cgaaaaagct taatccgata ccaacaagaa
    28081 ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc
    28141 accgtgtttc tgggtaaaca aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc
    28201 catgccttcc gcgtcgcgac gggcgatttc cggactgacg cccaacacca acaagaaatt
    28261 ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa
    28321 aaacacgcca cgccagggga tcatttcaat cagccccatg gttgccagcc gcttaagcat
    28381 tttagccacc gtcggttgcg aaactcccag acgagcagcc atgtccacct gacgagcttc
    28441 ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg
    28501 atgcgcctct ctgacctggc ggaacccttc aacgtgctct tccacgttca ctaactgcgt
    28561 cacttttttt gctattggcg tacctgcgcg acgactcatt gtgcttcctc attatggtga
    28621 cgcatacata gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa
    28681 aaattaacgt tttagcaata gctatataat atagcctgtg ctatatctgt atgtaatgca
    28741 atcatccctc aaggatcgac gggattagca agtcaggagg tcttatgaat gagttcaaga
    28801 ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg gttaatgctg ctctctatgt
    28861 tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc ttcagataaa tagcggcgtc
    28921 gcggtacgcc gcttcactcc tgcttttatg caggcataac gcgttttggt ctgaaaaaaa
    28981 ccactttttg tcggatttgc aatccccttc gcaaaagatt tgttcgtcag tagttgacct
    29041 gaacggcggc tcgctctatc ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga
    29101 cccctttccc ctcttcacgc actcttgcag gtattgaccc ttgacgccag ggtaagcaca
    29161 tggcgtttgt tacgatagtg gcatatgaat ttaaccctca aagaatcgct tgttacccgt
    29221 agccgggtat ttagcccgtg gactgcgttc tactttttac agtcgctatt aattaacctc
    29281 ggcttaggtt accccttcag tttgctctac accgctgcgt ttacggctat tttgcttttg
    29341 ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg gtgtcagttc gctggtggcg
    29401 gcttgttatt tcccttttgc tcaggcctac ggcgcgccta atttcaatac attgctggca
    29461 ttgcactcca ccaatatgga agagtcgacc gaaatcctga cgatttttcc gtggtacagc
    29521 tacctggtcg gcttatttat ttttgcgctc ggcgtaatag caatcaggcg aaaaaaagag
    29581 aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc tggtattcag tgtggcgaca
    29641 ttttttgttg ctcccgtgca aaacctggcc tggggtggcg tatttaaact gaaagatact
    29701 ggctatccgg tatttcgttt tgctaaggat gtcatcgtca ataataacga ggtgattgaa
    29761 gagcaagaac ggatggcaaa actttccgga atgaaagata cctggacggt cactgccgtt
    29821 aagccgaagt atcagaccta tgtggtagtg atcggtgaaa gcgcgcgtcg cgatgccctc
    29881 ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa cggtttgata
    29941 tttgctgact acattgctgc cagtggctcc acgcagaaat cgcttggctt aacgctcaat
    30001 cgcgtagtcg atggcaaacc acagtttcag gataactttg tcaccctggc aaatcgcgcg
    30061 ggcttccaaa cctggtggtt ttccaaccag ggccaaatcg gcgaatacga tactgctatc
    30121 gccagcatcg ccaaacgtgc agatgaagtg tacttcctga aagaaggtaa ttttgaagca
    30181 gataaaaaca cgaaagacga agcgttactg gatatgaccg ctcaagtgct ggcgcaagag
    30241 cactcgcaac cgcagctgat tgttctgcat ctgatgggct cgcatccgca ggcctgcgac
    30301 aggacacaag gcaaatacga aacctttgtg caatcgaaag aaacgtcgtg ctatctctat
    30361 accatgacgc aaacggacga tttactgcgc aagctgtacg atcagttacg caacagcggc
    30421 agcagcttct ctctggttta cttttctgac cacggtctgg cctttaaaga gcgcggcaaa
    30481 gacgtgcaat accttgccca tgatgataaa tatcagcaaa atttccaggt gccttttatg
    30541 gtcatttcca gcggcgataa agcgcatcgg gtgattaaag cccgccgctc agccaatgac
    30601 ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa tatcaaatac
    30661 ccgtttatat ctgagaagaa agccgggccg atatacatca ccaacttcca gttacagaag
    30721 gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa acaaaaatcc
    30781 gccccgagag gcggattttt atatcaccaa agtgattaga agcggtaacc aacaccggca
    30841 atccaagtgc ctacgtcaac gctacgaata cggctctgct cgtaagagaa gtccagagca
    30901 acgttttcca tcgggttgaa ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg
    30961 tcgtgtttgt aggtcgggta ttcagtggtc tggaatttac cataacccac acccactaca
    31021 ccgtagatgc ttgcccagtc gttaatgcgg taagccggac cagcagtgat gccgtagtac
    31081 tggtttttgt tgtagtcacc agagcttgca gtacggcttt tctcggtgta agtgaaagaa
    31141 ccgatcacac ccagcgggct gttgtcttct tcatagcggt atttcaggtt gaaaccgccc
    31201 attttgttca tttggccctg agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc
    31261 gcagctacgg aagtacctgc ggtgaaagcc agaactgcgg ccagtgctga aagacatgca
    31321 atttttttca taaccacctc aaatgtgatt caaataagtc ctaagtttta aatatatcaa
    31381 aaattaatgg gaaactcttc gcgatttgtg atgtctaacg ggccatttca tgtaacagaa
    31441 cgtttccata caccgctatc catctaaatt taaatcactt tttcagagaa ctgcgtaagt
    31501 attacgcatg ttttccctgt cattcatcca gattattcct aatcaccaga ctaatgattc
    31561 catcaatcct ggcgcatttt agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc
    31621 agcattgggt atatccagta cactccacgc tttacttaag tctagatatt tgtgggagaa
    31681 aggatgcctg gttcattacg taaaatgccg gtctggttac caatagtcat attgctcgtt
    31741 gccatggcgt ctattcaggg tggagcctcg ttagctaagt cactttttcc tctggtgagc
    31801 gcaccgggtg tcactgcgct gcgtctggca ttaggaacgc tgatcctcat cgcgttcttt
    31861 aagccatggc gactgcgctt tgccaaagag caacggttac cgctgttgtt ttacggcgtt
    31921 tcgctgggtg ggatgaatta tcttttttat ctttctattc agacagtacc gctgggtatt
    31981 gcggtggcgc tggagttcac cggaccactg gcggtggcac tgttctcttc tcgtcgcccg
    32041 gtagatttcg tctgggttgt gctggcggtt cttggtctgt ggttcctgct accgctgggg
    32101 caagacgttt cccatgtcga tttaaccggc tgtgcgctgg cactgggggc cggggcttgt
    32161 tgggctattt acattttaag tgggcaacgc gcaggagcgg aacatggccc tgcgacggta
    32221 gcaattggtt cgttgattgc agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt
    32281 gaagcactct ggcactggtc ggttattcca ttgggtctgg ctgtcgctat tctctcgacc
    32341 gctctgcctt attcgctgga aatgattgcc ctcacccgtt tgccaacacg gacatttggt
    32401 acgctgatga gcatggaacc ggcgctggct gccgtttccg ggatgatttt cctcggagaa
    32461 acactgacac ccatacagct gctggcgctc ggcgctatca tcgccgcttc aatggggtct
    32521 acgctgacag tacgcaaaga gagcaaaata aaagaattag acattaatta aatttacatt
    32581 tctgtatggt tatgcataac catacagaat ttctcgctac ttttcctcta cac
//
LOCUS       DAEEDS010000042        32145 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEEDS010000042 DAEEDS010000000
VERSION     DAEEDS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32145
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.042"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="KLH32_002146"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="KLH32_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /locus_tag="KLH32_002147"
     CDS             1130..1237
                     /locus_tag="KLH32_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0138968.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="KLH32_002148"
     CDS             complement(1290..2048)
                     /locus_tag="KLH32_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBE0138969.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2335..3264
                     /gene="pfkB"
                     /locus_tag="KLH32_002149"
     CDS             2335..3264
                     /gene="pfkB"
                     /locus_tag="KLH32_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBE0138970.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3365..3655
                     /gene="ghoS"
                     /locus_tag="KLH32_002150"
     CDS             3365..3655
                     /gene="ghoS"
                     /locus_tag="KLH32_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBE0138971.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3761..4621
                     /locus_tag="KLH32_002151"
     CDS             3761..4621
                     /locus_tag="KLH32_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBE0138972.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4662..5198)
                     /locus_tag="KLH32_002152"
     CDS             complement(4662..5198)
                     /locus_tag="KLH32_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBE0138973.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFIGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5345..6013
                     /gene="hxpB"
                     /locus_tag="KLH32_002153"
     CDS             5345..6013
                     /gene="hxpB"
                     /locus_tag="KLH32_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBE0138974.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6176..6766
                     /locus_tag="KLH32_002154"
     CDS             6176..6766
                     /locus_tag="KLH32_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0138975.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6899..8289
                     /gene="tcyP"
                     /locus_tag="KLH32_002155"
                     /pseudo
     CDS             6899..8289
                     /gene="tcyP"
                     /locus_tag="KLH32_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8293..9109)
                     /locus_tag="KLH32_002156"
                     /pseudo
     CDS             complement(8293..9109)
                     /locus_tag="KLH32_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9386..9649)
                     /gene="cedA"
                     /locus_tag="KLH32_002157"
     CDS             complement(9386..9649)
                     /gene="cedA"
                     /locus_tag="KLH32_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBE0138976.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9832..12093
                     /gene="katE"
                     /locus_tag="KLH32_002158"
     CDS             9832..12093
                     /gene="katE"
                     /locus_tag="KLH32_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBE0138977.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12140..12898)
                     /gene="chbG"
                     /locus_tag="KLH32_002159"
     CDS             complement(12140..12898)
                     /gene="chbG"
                     /locus_tag="KLH32_002159"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBE0138978.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12911..14263)
                     /gene="celF"
                     /locus_tag="KLH32_002160"
                     /gene_synonym="chbF"
     CDS             complement(12911..14263)
                     /gene="celF"
                     /locus_tag="KLH32_002160"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE0138979.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14368..15210)
                     /gene="chbR"
                     /locus_tag="KLH32_002161"
                     /pseudo
     CDS             complement(14368..15210)
                     /gene="chbR"
                     /locus_tag="KLH32_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15218..15568)
                     /gene="chbA"
                     /locus_tag="KLH32_002162"
     CDS             complement(15218..15568)
                     /gene="chbA"
                     /locus_tag="KLH32_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBE0138980.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15627..16976)
                     /gene="chbC"
                     /locus_tag="KLH32_002163"
     CDS             complement(15627..16976)
                     /gene="chbC"
                     /locus_tag="KLH32_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBE0138981.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17061..17381)
                     /gene="chbB"
                     /locus_tag="KLH32_002164"
     CDS             complement(17061..17381)
                     /gene="chbB"
                     /locus_tag="KLH32_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBE0138982.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17680..18018)
                     /gene="osmE"
                     /locus_tag="KLH32_002165"
     CDS             complement(17680..18018)
                     /gene="osmE"
                     /locus_tag="KLH32_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBE0138983.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18220..19047
                     /gene="nadE"
                     /locus_tag="KLH32_002166"
     CDS             18220..19047
                     /gene="nadE"
                     /locus_tag="KLH32_002166"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBE0138984.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19277..20164
                     /gene="cho"
                     /locus_tag="KLH32_002167"
     CDS             19277..20164
                     /gene="cho"
                     /locus_tag="KLH32_002167"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBE0138985.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20124..20699)
                     /gene="ves"
                     /locus_tag="KLH32_002168"
     CDS             complement(20124..20699)
                     /gene="ves"
                     /locus_tag="KLH32_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBE0138986.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20902..21387)
                     /gene="spy"
                     /locus_tag="KLH32_002169"
     CDS             complement(20902..21387)
                     /gene="spy"
                     /locus_tag="KLH32_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBE0138987.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21717..22685)
                     /gene="astE"
                     /locus_tag="KLH32_002170"
     CDS             complement(21717..22685)
                     /gene="astE"
                     /locus_tag="KLH32_002170"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBE0138988.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22678..24011)
                     /gene="astB"
                     /locus_tag="KLH32_002171"
                     /pseudo
     CDS             complement(22678..24011)
                     /gene="astB"
                     /locus_tag="KLH32_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24008..25486)
                     /gene="astD"
                     /locus_tag="KLH32_002172"
     CDS             complement(24008..25486)
                     /gene="astD"
                     /locus_tag="KLH32_002172"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBE0138989.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25483..26517)
                     /gene="astA"
                     /locus_tag="KLH32_002173"
     CDS             complement(25483..26517)
                     /gene="astA"
                     /locus_tag="KLH32_002173"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBE0138990.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26514..27734)
                     /gene="astC"
                     /locus_tag="KLH32_002174"
                     /pseudo
     CDS             complement(26514..27734)
                     /gene="astC"
                     /locus_tag="KLH32_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28180..28986
                     /gene="xthA"
                     /locus_tag="KLH32_002175"
     CDS             28180..28986
                     /gene="xthA"
                     /locus_tag="KLH32_002175"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBE0138991.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29153..29861
                     /locus_tag="KLH32_002176"
                     /pseudo
     CDS             29153..29861
                     /locus_tag="KLH32_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29866..30543
                     /gene="ydjY"
                     /locus_tag="KLH32_002177"
     CDS             29866..30543
                     /gene="ydjY"
                     /locus_tag="KLH32_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBE0138992.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30558..31265
                     /locus_tag="KLH32_002178"
     CDS             30558..31265
                     /locus_tag="KLH32_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE0138993.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31265..31813
                     /locus_tag="KLH32_002179"
     CDS             31265..31813
                     /locus_tag="KLH32_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBE0138994.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31823..>32145
                     /locus_tag="KLH32_002180"
     CDS             31823..>32145
                     /locus_tag="KLH32_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0138995.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8247 a         8242 c         7613 g         8043 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttccagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattaac cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     2161 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     2221 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     2281 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     2341 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     2401 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     2461 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     2521 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2581 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2641 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2701 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2761 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2821 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2881 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca
     2941 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     3001 gtttcccttg gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg
     3061 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     3121 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     3181 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     3241 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     3301 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     3361 aactatggcc agtggcgatc ttgtccgtta tgtcataacc gtcatgttgc atgaggatac
     3421 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     3481 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3541 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3601 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3661 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3721 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3781 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3841 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3901 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3961 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     4021 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     4081 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     4141 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     4201 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     4261 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     4321 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     4381 cattttcgac cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct
     4441 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     4501 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4561 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4621 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4681 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4741 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4801 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4861 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgat aaaaatcagg
     4921 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4981 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     5041 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     5101 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     5161 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     5221 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     5281 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     5341 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     5401 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     5461 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     5521 tctttggtac gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt
     5581 tattgcccgt gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga
     5641 agctgttgcg ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact
     5701 acatatgctg gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc
     5761 ctcggctgaa aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc
     5821 aaaactgggc gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat
     5881 cgcttctaaa gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga
     5941 tccacgtttt gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga
     6001 ccttctcggt taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt
     6061 gcattcaact gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc
     6121 gtgcactgta taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     6181 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     6241 gacgcccgtg ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg
     6301 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc
     6361 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt
     6421 tgcgctgctg gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc
     6481 gctacaagga atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     6541 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     6601 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     6661 gccccattca ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     6721 gcagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca
     6781 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     6841 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     6901 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     6961 gacccgccat aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt
     7021 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     7081 tgtacagtgg tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     7141 gccgttagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     7201 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct
     7261 ggtcggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     7321 tgcagaaact gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca
     7381 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg
     7441 agccaatccg acgtcaatca tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     7501 attgaaactg ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac
     7561 cctacaaagc tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt
     7621 tctggctcta atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg
     7681 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct
     7741 gctgggcatt aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac
     7801 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     7861 gcgtcgtctg ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat
     7921 tggtcagaac ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg
     7981 gttggcatta acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     8041 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg
     8101 cctgcgatgg gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc
     8161 gacatgggcc gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc
     8221 cagtggctga agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca
     8281 caccgttaat tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt
     8341 taaatgttcc atcgcggcga tgcatactga ctcatattta aacagatcgt cagcgaaaaa
     8401 aatgtccaac ttatcaatac attcctggct caatgaatgt gcctgtagtg agaagcttaa
     8461 aacctcatac ccgagacgtt ttctaagtga actatctgga atactcataa tatcttcaag
     8521 cacaccactt cccattcccg gaagatttag tggattaaaa agaagattga tattaagcat
     8581 tgatattatc gaaggaaact ttaggagact ctcatttttt ttgaatcgca gcaaaaatgc
     8641 tcccggcagg atgtatataa tttttttcat agtatatagt ccttaaataa tgaataacca
     8701 acctattttt tatattgatc aaattcgtca ttttttcggg tcggaattca caggtaaaat
     8761 ccatcccaac aaaattctca ttaatatctc ttatccgaaa ctcactgcca taaagattat
     8821 taattatcac tctatattta acattgtcct gcagatacgt atcaaaacta agtgctggcg
     8881 ctcctagctt tgaggcatca tagttaatgt tagacatata ggcaatcata cgattgaagt
     8941 tcgactctgc tatttcatcg cagtcacatt gacccgttga tataaagaaa gtgagtgccg
     9001 ccatggtaga ctcaagcctt tctttaaaag agtcgaaagc caggctttta atatttaaat
     9061 caccataatt actctgtatt aagtttgtag aaaacatctc ccgcctcata ttgttaacaa
     9121 aattattatc ttatttaaat ctaagtcatt tacaatataa gtttaagagc gacgccacag
     9181 gatgaactat caaaaataac tcatcatgat tagcaaaact taaccatttt aaaataaata
     9241 aataattaaa gaaaaaagat cacttattta tagcaatagc tcgtcaaagg cagctttttg
     9301 ttacaggtgg tttgaatgaa tgtagcaacg aaatgcagaa tttcaggtcc tgtaactccc
     9361 ggcaaaaccg ggaggtatgt aatccttact cagtcacttc cccttcctgg cggatctgat
     9421 ttgcccaacg ttgggcagat tcaggcacag taaacgccgg tgagcgcaga aatgactctc
     9481 ccatcagtac aaacgcaaca tatttgccac gcagcatcca gacatcacga aacgaatcca
     9541 tctttatcgc atgttctggc ggcgcgggtt ccgtgcgtgg gacatagcta ataatctggc
     9601 ggttttgctg gcggagcggt ttcttcatta ctggcttcac taaacgcata ttaaaaatta
     9661 gaaaaactgt agtttagccg atttagcccc tgtacgtccc gctttgcgtg tatttcataa
     9721 caccgtttcc agaatagtct ccgaagcggg atctggctgg tggtctatag ttagagagtt
     9781 ttttgaccaa aacagcggcc ctttcagtaa taaattaagg agacgagttc aatgtcgcaa
     9841 cataacgaaa agaacccaca tcagcaccag tcaccactac acgattccag cgaagcgaaa
     9901 ccggggatgg actcactggc acctgaggac ggatctcatc gtccagcggc tgaaccaaca
     9961 ccgccaggtg cacaacctac cgcccctggg agcctgaaag cccctgatac acgtaacgaa
    10021 aaacttaatt ctctggaaga cgtacgcaaa ggcagtgaaa attatgcgct gaccactaat
    10081 cagggcgtgc gcatcgccga tgatcaaaac tcactgcgtg ccggtaaccg tggtccaacg
    10141 ctgctggaag attttattct gcgcgagaaa atcacccact ttgaccatga gcgcatcccg
    10201 gaacgtattg ttcatgcacg cggctcagca gctcacggtt atttccagcc atataaaagc
    10261 ttaagcgata tcaccaaagc ggatttcctc tcagatccga acaaaatcac cccagtattt
    10321 gttaggttct ctaccgttca gggtggagct ggctctgccg ataccgtgcg tgatatccgt
    10381 ggttttgcca ccaagttcta tactgaagag ggtatttttg acctcgttgg caataacacg
    10441 ccaatcttct ttatccagga tgcgcataaa ttccccgatt ttgttcatgc ggtaaaacca
    10501 gaaccgcact gggcaattcc acaagggcaa agtgcccacg atactttctg ggattatgtt
    10561 tctctgcaac ctgaaactct gcacaacgtg atgtgggcga tgtcggatcg cggcatcccc
    10621 cgcagttacc gcaccatgga aggcttcggt attcacacct tccgcctgat taatgccgaa
    10681 gggaaggcaa cgtttgtacg tttccactgg aaaccactgg caggtaaagc ctcactcgtt
    10741 tgggatgaag cacaaaaact caccggacgt gacccggact tccaccgccg cgagttgtgg
    10801 gaagccattg aagcaggcga ttttccggaa tacgaactgg gcttccagtt gattcctgaa
    10861 gaagatgaat tcaagttcga cttcgatctt ctcgatccaa ccaaactgat cccggaagaa
    10921 ctggtgcccg ttcagcgtgt cggcaaaatg gtgctcaatc gcaatccgga taacttcttt
    10981 gccgaaaacg aacaagcagc attccatcca ggtcatattg ttcctggtct ggacttcacc
    11041 aacgatcccc tgttgcaggg acgtttgttc tcctataccg atacacaaat cagtcgtctt
    11101 ggtgggccga atttccatga gattccgatt aaccgcccga cctgccctta ccataatttc
    11161 cagcgtgacg gcatgcatcg tatggggatc gacactaacc cggcgaatta tgaaccgaac
    11221 tcgatcaacg ataactggcc gcgcgaaaca ccaccggggc cgaaacgcgg cggttttgaa
    11281 tcataccaag agcgcgtgga aggcaataaa gttcgcgagc gcagcccatc gtttggcgaa
    11341 tattattccc atccgcgtct gttctggcta agtcagacgc cattcgagca gcgccatatt
    11401 gtcgatggtt tcagttttga gttaagcaaa gtcgttcgtc cgtatattcg tgagcgcgtt
    11461 gttgaccagc tggcgcatat tgatctcact ctggcccagg cggtggcgaa aaatctcggt
    11521 atcgaactga ctgacgacca gctgaatatc accccacctc cggacgtcaa cggtctgaaa
    11581 aaggatccat ccttaagttt gtacgccatt cctgacggtg atgtgaaagg tcgcgtggta
    11641 gcgattttac ttaatgatga agtgagatcg gcagaccttc tggccattct caaggccatg
    11701 aaggccaaag gcgttcatgc caaactgctc tactcccgaa tgggtgaagt gactgcggat
    11761 gacggtacgg tgttgcctat agccgctacc tttgccggtg caccttcgct gacggtcgat
    11821 gcggtcattg tcccttgcgg caatatcgcg gatatcgctg acaacggcga tgccaactac
    11881 tatctgatgg aagcctacaa acaccttaaa ccgattgcgc tggcgggaga cgcgcgcaag
    11941 tttaaagcaa caatcaaggt agctgaccag ggtgaagaag ggattgtgga agctgacagc
    12001 accgacggta gttttatgga tgaactgtta acgctgatgg cagaacaccg cgtgtggtca
    12061 cgcattccta agattgacaa aattccggcg taaatatgga taaggtcacg tcccctgggg
    12121 agtgacctta ccaccttact tacacatcaa gataactacc caaccgataa ccacgctcag
    12181 caattgcata tttcaacgac gctgatgtca gcacttccag ctccgttaac cgtgggaaac
    12241 agtaagcact ctgacggata gtattatcga taaacgccgg atgacacatc acctccagcg
    12301 aaggatcacc ccgatggctt gcgtcatcca gcacttgcag gaacagtgcc tcgctaatct
    12361 cttcaccata aaacgcgcta ctgaatccct gcgaactgcg cagattaacc ggtaaatcac
    12421 cagcgttaga tagcggctga cggtcgatgc gcaacgcaat cccctcttca gccgcaaacc
    12481 tggcaacaat cgggaaaatc tgcgggaaca tatgcacatg atgatggcta tcaagatgcg
    12541 taggtttgcg tccaaatagc tcaatgaaac gcaaatactg actggcaagc tcctgagtaa
    12601 tttcttccag cggtaaagca tcttcttctg ccaactgcca gatccatttt cccagcacac
    12661 catcgcgggt taaccccggc atagctgtca gtggcttgcc catagtaagg acaaagtgca
    12721 tccctatggc cagactcggt tcatcacgac tcaactgcac cgcatggtca atagcctgcc
    12781 cattcaccag cgccgtcgtc gatgtgacaa tcccattgcg acaggcctcg ataatgccgt
    12841 agttctgacc tttgcttaag ccaaaatcat cggcattaac aatcagtaag cgttccataa
    12901 tcagcctcgg ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa tttggcagcc
    12961 atttctcgtg cgccagaatc atctcgcgtg ccagcagctc agcatcgcga tcggaatgca
    13021 ccaacggact aaggtttagc gccagtaaca catcgttaaa ttctccgcta agtgcggcgt
    13081 tgctggcagc aatctcgaag cctttaatgg tgtgaatcag ccccatcact ttatcatcga
    13141 aatgcgtaat gcgcggatgt ggcgtcgcgc catcgtgccc cagcttacag gtcatttcta
    13201 ccgcccagtc tgccggaata ttatcaatat gcccatgatg cgggatatta acgtaatgtt
    13261 cagcttgctt gtcgttgtag atagcgttga tcacttcgca cgctgcatca gagtaataag
    13321 ccccaccgcg ctgttccagt tctttcggct taactttcaa ctccgggttt ttatacagct
    13381 caaaaagttg tttctcgact ttctgtacta cctgcgctcg tgcgccgcct ttgtagtatt
    13441 cgcccatttc aatagccagc atctcttttt gcttgaagta ataaagcaaa taagaacacg
    13501 gcaacagatt cagagaacga attaagcctt cgctaaatgg cagatcgaaa atatttttaa
    13561 cgccagatac ttttaactgc ccggatgcca caccatcaag caattcggca aagcgcgact
    13621 tgccatttac cagcacatcc ttaatgaaca ccatatggtt gaggccaaac aaatcgatag
    13681 ataaatcatc gctgtctttc agcatcagaa catcgtgaat aaacatcttc atgccgatcg
    13741 gaatattaca cacgccgata aaacgtttaa atccggtatg acgataaacg gcttcagtaa
    13801 ccattcccgc cgggttagtg aagttaatca cccatgcatt cggacaaagt tcttcgacat
    13861 cttttacgat gtcaaaaatc accggaatgg tacgcagacc tttaaacaaa ccgcccgcgc
    13921 cgttggtttc ctgaccaaga taaccatgac ttaatggaat acgttcatcc agttcacgcg
    13981 ccggtaattg gccaacgcgc agttgggtag taacgaaatc agcatctttc aatgcttcgc
    14041 ggcaatccag cgttttataa agcttcatcg ggacgccagc gttatcaatc atccgttggc
    14101 agagatcgaa aataatatcc agtttagctt tgccaccctc gacatccacc agccataatt
    14161 cgctgaccgg caattcgtga taacgcttaa taaatccttc cagtaactcc ggggtatagc
    14221 tgctcccgcc accaatagtg acgactttta atttctggct cataatttct cccttcagta
    14281 cagaatactg atatctggca tatctgcccc cccggacata aataatccag caacaggaca
    14341 gatatgtgaa ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata
    14401 tctcttaggc gtaaaggacg ttaatttctt aaacgtttta ataaacaaac tcgggctact
    14461 ataaccggcc tcaaacgcaa tatccgtcac ggaatagttg gtcatttcca gttgtttttt
    14521 tgcaaaatta atacggatct cattaataat ctgcattggt gttttgccat aatatcgttg
    14581 agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc
    14641 cgattcacta aactgctctt tatcatgcat cttttctacc gtacttttca gccactacgg
    14701 tacatcatca atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt
    14761 aaccatctca acaaactctt ccagccctgt ttcgcggaaa tttaatgaag aaatcactgt
    14821 ttccacatag gtgagaaaag cgttattggt ccggtatacc tgcgaagcaa caaagcaata
    14881 aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat
    14941 gcgcgtggca ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa
    15001 atcgccccgt tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa
    15061 tactagagta aattcataat agtcgtgctg atgcagtccg ctgatactct cagttttgtt
    15121 atagataaac acatggaagt ttttgccatt aaacaactgc tgttctcggg cagtggcaat
    15181 ttccggcgcg ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa
    15241 gctcaattaa ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag
    15301 cgtggaccag caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt
    15361 tcgtctgtac cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggctttcg
    15421 ctgcggcaaa atcgccctgc ttcgcctgtt tcaatgcggc atacgccagg ctacgcgctt
    15481 gtccagagtt gatgataagc cccatcacca cttcttccag ctcttcagct tctgtttgcg
    15541 tatcaggaat attatcgaga tccatcatac atcgttcctc tctcttctta ccggcaagat
    15601 tacccgtacc ggcatcgatt aaaatttcag gggttagcga tatcttcttc gctctcttct
    15661 ttatcaatcg cattttgtgc tttgttagcc accacaacaa agggcagata aattaacgtt
    15721 gcgatgccaa ggttgaagag tgcgaccagc aatgcggcga cgctaccgtt ggtgttaaag
    15781 aaggctccca gaccggttgg catggtccac ggtgcaatat tggtcaccgg aggaatgatg
    15841 cccatgtagt acgctgcgag ggtgattgcc gccagaatcg gttgtaccag tacaaacggg
    15901 ataaacatca ccgggttcat gataattggc agaccaaaca gaatcggttc gttaatctgg
    15961 aagatgccgg acggcagcgc cagttttgcc acctgacgat aatcagcacg gcgagaggcg
    16021 ataaagatag ccaggatcag gcctaaagtc gcaccactgc ccccaaggaa aataaaggag
    16081 tccagcatcg gcttggccca gatatggaag gtcttaccgg ctgccagcgc cgcttcgacg
    16141 gaaccatatt gctgataggt cgcgatattt tccagcgccc acggcgtcat aatgccgttg
    16201 tccagtgcgg tcagcgccag cgcgccatga ataccgaaga accagagcag tggaacaaag
    16261 atcacatagg cccagcccac cacgctaccc aacgatgcca gtggggttga gatggtatcc
    16321 ataatgatct gatggaagtt ggtgccccag gtattcaacg cccaggcaat aatccccatc
    16381 acggaaagaa taataaagcc gggaattaat gccgagaaag aacgcgatac tgaagcaggt
    16441 acgctgtcgg gcagtttaat gacccaattg cggcggacaa taaaggtaaa catttctgcc
    16501 accaccaggc caataataat cccggagatg atattcgccc cacctaacca gtttgcgcca
    16561 accgcatagg cctcaccgac actatatggg gtgacggtca taaatgctgc aacggataac
    16621 aacccagccg ccagcgcatc gactttacgc tcttctgcca gcgccatgcc aataaagaac
    16681 ggtgccatta aagacattat tcctaatgtt ccgttatata cgttgccgcc aatacctttc
    16741 agaccattaa gtgtttcaat ggttgaggcg tcgaggcgaa tacctaagga ataaaaaaac
    16801 gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc ccccgcaagg
    16861 gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg ctgctttcct
    16921 attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt actcatagaa
    16981 aaataccctt aaaaaccgca atttaaatat tacggtattg atttatgaaa taactctttg
    17041 acgggaaaat ttaaaataaa ttaatttgct gcggcttttt taatcgctgc aaccgcagcc
    17101 ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac ttcaaccggt
    17161 ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg ccctaataac
    17221 acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc ttcaataatg
    17281 accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa agagatagac
    17341 atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg
    17401 attatctgtc agccagacac tccgcaagcc ttgacctgct tccatgctct gggtaacttg
    17461 cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtatagac ctgagcaata
    17521 gtatttcttt gaccttataa attgtttctc cttgacctgt ggttatgacc ctcttcacat
    17581 ttcgggcaaa taattcgcga agataattaa gaactcaggc caaaaaaaac cggcgcaatg
    17641 gccggtttcc gttgttactc aagctttcag acgaattgat tacttcgcag cctgtggatc
    17701 agtgtcgtat tcagcacagg tctgataacc ggagttgatg acatgtccgg tatcatctaa
    17761 cgcgacaaag taggtttctg ctttaccatc acgttgaccc aggatgtagg tctggcaagt
    17821 accgcgagca tggatcatgc tcacttcaga cgaaggttta cccgcaattt gtgcaacctg
    17881 cgcccggctc atgccttttt tgacgtcttt caccacaggc tgtacaaact ggtctttggt
    17941 acgatcataa gctgtacaac ccgccagcat ggttaatacc gccgctgcac tcagaattcc
    18001 tgccatattc ttgttcatat tccgtcctct tgtttatcag cgtgttagat aagcctggaa
    18061 tacattgggc gctttttcaa gcccgtgaac gaaacggctc cgctttcaga ggattcctgt
    18121 atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc aacgggttag
    18181 ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa tgacattgca acaacaaata
    18241 ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag aggaaattcg tcgtagtgtc
    18301 gattttctga aaagctacct gcaaacttat ccgttcatta aatcactggt gctcgggatc
    18361 agcggcggtc aggactccac gcttgccgga aagctgtgcc agatggcgat taatgagctg
    18421 cgccaggaaa ctcgcaacga atcactgcaa tttattgccg tacgcctgcc ctatggtgtt
    18481 caggctgacg aacaagattg ccaggatgcc attgccttta ttcaaccgga tcgcgtatta
    18541 accgttaata tcaagggcgc ggtattggct agcgagcagg cattgcggga agcaggcatt
    18601 gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat
    18661 agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt
    18721 accggattct tcactaaata tggtgacggc ggtacggata ttaatccgct gtatcgtctc
    18781 aacaaacgcc agggtaaaca gttactgacg gcattaggtt gcccggaaca cctttataag
    18841 aaagccccaa ctgccgatct tgaagatgat cgcccttctc tgccagatga agtggcactc
    18901 ggcgtgacct atgacaatat cgacgactat ctggaaggga aaaacctacc tgaacaggtc
    18961 gccagaacaa tagagaactg gtatctgaaa accgaacata aacgccgtcc gccaattaac
    19021 gttttcgatg atttctggaa aaagtaataa tttgcaccta ttggattgtg cgaaaaagag
    19081 taatttgttc acgccggatg cggcgtgaac gccttattcg acctacaaaa ctatgcaaat
    19141 tcaatacatt gcaggagtcg aataggcctg acaggcgtag cacgtcagac ggtgtaacct
    19201 ttgtcatcaa cccgcctctt ttttaatcgc ctcccgcctg ttacactgga tagataacca
    19261 gcattcggag tcaacagtgg tacggcgttt aacttctccg cggctcgaat ttgaagctgc
    19321 ggcaatttat gaatatcccg aacatttaca ttcattcctt aatgacttac ccacccgacc
    19381 aggggtgtat ctgtttcatg gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt
    19441 taatatccgc agtcgggtgc tttctcattt acgcaccccg gatgaagccg ccatgctacg
    19501 gcaatcccgg cgaattagct ggatctgcac cgcaggcgaa atcggcgctc tgctccttga
    19561 agcgcgatta atcaaagaac aacagccgct gtttaataaa cggttgcgcc gcaatcgcca
    19621 gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga
    19681 tttttcacga gcccccaacc tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc
    19741 attgcagagc atcgctgatg aacaaaaact ttgttatggc ctgttgggac tggaaccgtt
    19801 aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg
    19861 taaagagagc catgaggaac atgcgctacg cttgcgccag tctctggagc gtttgcgagt
    19921 ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa cagcacccgg aaatgactca
    19981 atatcatatt attcaaaact ggctgtggct gggggcggtt aattcgctgg aagaagcgac
    20041 aacgttaatt cggacacccg ccgggtttga tcacgacggt tataaaattc tttgtaagcc
    20101 gctgctttcc ggtaactatg aaattactga acttgatccg gcgaatgacc agcgagccag
    20161 ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca
    20221 tcgaaccagc acgcgccctg gtcggtagtg agcagtttgt cgcccagttg ccacgccccg
    20281 ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt
    20341 ctgactttcg ccttacagac atccaggcgc gtcataatat tgaaatccat cgacatctgc
    20401 cctgccgtca gtttcgcttt taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt
    20461 tttaaggtat ggttaaagcg gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac
    20521 gtcactatcc tttccatgcc gggaaacaaa gaaaactccc cattcgccgc gatggatgca
    20581 atgctggcac gccagtaaaa atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt
    20641 tcaccggcag cgtttcgcca cagattcacc gacattttac gcatatcaaa gtattccata
    20701 ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt aaccgtggat gattatcggc
    20761 aactcgggtg atttacgtta gtggtgatca ggcttttttt acaactgttt gagaagagaa
    20821 aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc
    20881 ttaagttttt tggctgaaag attattcagc agttgcaggc attttacctt ttgccgctgg
    20941 acgttctgtc agacgcttct caaaattagc attaaattgc tttttctgtt ccggcgtcag
    21001 gatgttgtaa attttgttct gggtttccat gtgcgccagc atgttagctt tgcgctgttc
    21061 ttccattttt gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc tggcaatgat
    21121 gtcatgcatt gcgcggcgtt cttccagcgg cggacgtttc atctggtcac gctggccttt
    21181 catgatttcg tggatctgct gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat
    21241 gtcctgatgc ggaccgaact tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc
    21301 tgcggtagtg gtgtctgcgg catgggccag gttagccgcg ccaagagcca gggtagaggc
    21361 aacaaacagt gcagttaatt tacgcatatt ctatatcctt cctttcagtt atttattacg
    21421 gctttcttaa gtagcgtgcc gtgttgacga gattaacttt actgacttta gcgtcaatta
    21481 atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa
    21541 aaaaagaaaa tttgatggag agtgatgaga gaatattaca acacgatgat tttgcagaga
    21601 ttatgaagaa ctataccgga tgactggtga taaataaagc aaataaccag gattaatctg
    21661 tattaattta taagaaagca acttaatacc cgcagaatga tttctgcggg taagtattag
    21721 cttatttttt cgagcattaa tcccgcgcgt aatcccaacg ctaccaacgg attagggaat
    21781 aacacatact ctacatcatg ggttacggta aaacgttcct ctccgtcctg cgccagcaat
    21841 gttcctttct caaacggcat aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac
    21901 ggcgagtggc gagtaatttg cgaaaccacc cgataacgga gcggcggtgt tctcacgata
    21961 ccgacactct caccagatag cagcgcagca attgcgctgg cagttactgc aaactggcga
    22021 agatcgtttt gcccaaaggg caacgctttg ccaagttcca acgtacagac cagcgcgcca
    22081 aaatgtctga cgctgaaatg ggtaaacgta ccaccaggct cctgatggaa caccagcgcc
    22141 tccagccccg ccgcacccag ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc
    22201 ggtaatacac cgaactgcgg atgcaaggag ccacgaattg cggtatgtag atcaaggtgc
    22261 caacgcacag attctttgcc ctggtcataa aaatcttcca ggcactgttc cagttcgcgc
    22321 gcccgacagg tttctccgct ttcagcaaat agctgccaac gaccgccaaa cattcggttc
    22381 atatcgctat ggcaataacg tttcccttgc ttcagcgcag gaggattccc gaggatcacc
    22441 agcaaccgcc aacgtaacgg gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc
    22501 atttccacag gtgccgtctc attaccgtgg atccccgcag aaatcaccag cgcgccttgc
    22561 ggtggcgtta atggcgtcag ttccagcaca ccatcgccca gccagcgcca gcgaacgccg
    22621 ttgatttctc gctcggtgat aaccggtttt ttacccgtta aggtcagagc aagaaaatta
    22681 tccattgccc cctccctcgc gctggaacgg ataaaccgaa ccgagattca gtaattggct
    22741 caatacatcc agtgcttccc gcccttcgcg cagcaattgc gggtcggcca gatcggcagc
    22801 agtaagacga tcgcggtagt aacgatccac ccagtcattg agcacattaa acaaggtatc
    22861 gttcatcatc accgccggat tcaccgcccg gcgttcttct tctgtcaata ccccccgcaa
    22921 ccgcaggcac gccgggccgc cgccattcgc catgctttca cgtaagtcaa agacttttag
    22981 ttcactaatc gggttgtcag cggcaaggag ttcattgaga taaccccata ctccggcgtg
    23041 ctcccgacac tcctgaggca gcaccaacat catggaacca tcatcgcggc tcaacagttg
    23101 gctgttaaac agataggtag acaccgcatc cgacacggaa acctgagttg ccggaacttc
    23161 tatcgccata aaaccgttca cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt
    23221 tggtggcaaa acagcacctg gcggttactc acggcaatca cgtcattatg aaaaacgctc
    23281 tggtcgataa cgtccgggtt ttgctgggcg aaaatcacct gttggggatt cacctgatta
    23341 agccttgcca ccgcctcgct ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg
    23401 gtatcattgc cttcttctcg cccgtagaca aaaagtagca tacccggttc accgtaatga
    23461 ccgccgagac gattgtggtt tgccgccccc tcatcaccga gcaacgctac ctgcggcaac
    23521 gccgaatgga cgctaaattt ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg
    23581 acgggcgctt ccagcgaacg gtgaaattta ttgttcaggt tggcaacggt gagatgcact
    23641 ttgccatcca gcgtatcggc agatggcgcg atcgttgccg cattggctac ccacattggc
    23701 gaagcggaac tgacgctgga aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc
    23761 tgctcatcgc taccactgaa tcccaactga cgcagcaccg gaataaacgg acgctcgtgc
    23821 ggcgggatca cggcctgggg gaatcccgca tcggcaaggg ctttcatttt cagtaagccc
    23881 tgttttgccg ccagtcgcgg gttagacacc tgaaaacggt gacgggtaga ggcttcatta
    23941 ccaaacgaca ggcccgcgta atgatgcgtc agccctacca gcccgtcgaa attaacttcc
    24001 caggcgttca tcgcaccacc tcatcggaaa aatccagccc ggggttaagc gtggcgggca
    24061 atgttaacga gtccgactcc aggctcgcca taggccatgc gcagtaatct gcggcatacc
    24121 aggcgctggg gcgatggtta ccggaagcac caatgccgcc gaatggggcg gtactggcag
    24181 caccagtaag cggtttgttc cagttaacaa tccccgcccg cgcctccagc aacagttgat
    24241 cgaatttttc ccgctcgggg gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg
    24301 ccatccgaat cgcctcatca aaagtatcat aacgccagac gcgcaaaagc ggcccgaaca
    24361 cctcttcatc cggcacaccg ccaacgcccg tcatttcaat gatccctggt gtcagcaacg
    24421 atgtccctgc ttgtaataag cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt
    24481 gctgccaggc agtaaccacc tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg
    24541 gctgcggttc gtcatcccag ttgcccggcg ttaatcgctg gctgacggca accagactag
    24601 caagaaacgc atcgccctgc gctccgcttt tgagcaataa acggcgggcg caggtgcagc
    24661 gttgcccgac tgtgacaaac gccgactgaa tggtcagatg gacagccgcg tcgatatccg
    24721 ccacctcatc gataattagc ggattattac cgcccatctc aagggcgaga attttctccg
    24781 gctgaccgga gagctggcga tgcagctggt agccggtatt ggcgctaccg gtaaacagca
    24841 aaccgtcgag atcctccagc gcactcagcg cctgacccgt ttcacgcccg ccctgcacca
    24901 ggttcagcac gcccggcggc aagccagcct gctgccataa acgcattacc gcttcgccac
    24961 tccacggtgt cagttcgctg ggtttaaaga taatggtgtt acctgccagc aatgccggaa
    25021 cgatatgtcc gttcggcaaa tgaccaggga aattatacgg cccaaacacc gccagcacgc
    25081 cgtgcgggcg atgtcgcagg ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg
    25141 tacgaacgtg atacgcctta attgatatcg cgattttatt gatcatcgcc gtcacttcgg
    25201 ttgccgcttc ccagcgcggc ttacccgttt ctctggcaat aatcgcggtt aattcggctt
    25261 tattgctttc cagcaatccg gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga
    25321 gccgcgccca gcgcggaaac gccgcacggg ctgcccgaca agcctgttcg acctgagcgg
    25381 catcggcatc attgccttgc cataacacct cgcccgatac cggattacgc ttcacacgcg
    25441 atgcgccctg gcccgttatc cagtcaccgt taatccataa agtcatgctg ttttctcctc
    25501 tgcgcacagg cgcaccagac gaacgcgatc ccctgcgtgg catttgaggg catccagttg
    25561 tgcggcggtt aaaatcaaac gctcggttgc cggatcggta cgcgccagca ccacgcggaa
    25621 atggtgataa ttttcattgg cgaccaggca ggccggaaaa tcgccctgcg caggctgccc
    25681 ttctgccact tccaccagcc gacttttacg gatggcgcgc acgcggtcga tgtcacactc
    25741 aagcgttggc ccgccgtcga agatgtcgat atagttgcgg taacgaaaac cttctttctc
    25801 cagcaccgcg cgggcaggcg cggtttgcgg atgtacctga ccgatgacgt cctgggcttc
    25861 ctgggataaa aagtgggtat agatcggatg tttcggcatc agttctgcaa taaatgcctt
    25921 ttgcccggtg ccgcagagaa aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc
    25981 gaggctttgc cagaacggtg aatagccgtg ttcgtcaatc accccgcgca tttcagcaac
    26041 cactttgtca ttaaacttgt cgcgaaaagc cgccataaac ataaagcgcg atttcgacag
    26101 caaatagccg ttgccctctt tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct
    26161 gctgccggtg tgatcgttac taagaaacag cgtcggcaat gcgttataga cattcagctc
    26221 ttttgaggcg tgaaccaacg tgccgacgcg atagttgtac cagggatcgt tcagcccaac
    26281 cgccacctca atagcgcaaa tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa
    26341 cacatagccc tgctcgcttt tgggcagttc accttgccag gttttgattg ccctttcgat
    26401 acgcgccgaa agcgtggctt cattggcggg aagcgacgtc aggccgccgc ccgttttgct
    26461 ggcaagctgc atcagcgccg agacatctga gcgctcaacg ggacggatga ccatcatgat
    26521 gaacctcggc taacaaagtg ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc
    26581 tcttcttcgc tgacattgag cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt
    26641 accatcacgc ctgctttcgc cgcttccaga gagatctgtt tcgcttgccc ggcgtaatcg
    26701 gcattcagta cacagccaat caacaaacct aagccgcgaa cttcactgaa caaaccatag
    26761 cggtgattaa tagtattaag acgctcaaca aaccagtcgt gacgctgttt aacgccatta
    26821 agcatctctg gtgtgttgat gagctccagc actttgcctg ctaccgccga ggccagcgga
    26881 ttaccgccat aggtggtgcc atgagtgcca acggtcatca cgcgggcgca ctcttcggtt
    26941 gccaacaacg caccgaccgg gaaaccgccg cccagcgctt tggcggtggt taacagatcg
    27001 ggcgtcacgc cgtagtgcat ataggcatac agctcaccag tgcgcccgac gccggtttgt
    27061 acctcatcaa aaatcagcaa ggcattgtgg cggtcacaca attcacgcag accttgtaaa
    27121 aacgcgttgc tggctggcac cacaccgcct tcgccctaga tgggctcgac aatcaccgca
    27181 caggtggcgt cgtcaatcag cgcgctggca gagttaatat cgttatatgc agcatgacga
    27241 atatccgccg gcagtggcgc aaaatcctgt gaataggctg gctgcccacc cgcactgaca
    27301 gtaaacagcg tgcgaccatg aaacgcattt ttgaacgcca cgatgccact cttatggctg
    27361 ccgtagcggt cgtgagcgaa tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca
    27421 ccggagttac aaaagaagac gcgatcggca aacgtggcgt cgatcaattt tttcgccagt
    27481 cgcagtaccg gctcgttggt gtaaccgttg ccggtatgcc agaacttact cgcctgttcg
    27541 ttcagcgctt cacgcagttc cggatgcgca tggcccagcg cgttcaccgc aatgccaccc
    27601 gcgaagtcga tatactcttt cccctgctga tcccacaagc gcgaaccttc gccacgtacc
    27661 ggtataaagg gtgccggagc gtaaacaggt atcatccatt catcaaagtt ttcacgcgta
    27721 attggctgag acatagcgac ctctacagta aataattcgt tatttgcatg ttaacaataa
    27781 gtaatgtttg cgctgtaaat gtagattgca gggttcgtgc cagccagtga taaaagtgca
    27841 taaacggcgg aggctaactg gaaatcaagg agttataacc aaaccatatg catttaaagt
    27901 gcatataaag tgaatacgtt tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt
    27961 atgcagaagt aaaatataat tctagaattg tgatcattga cgaaatttac tggaaattac
    28021 tgcgccattc tgacgcagcg cgcaccaaaa gcgggcattt tttgcgccat cattgacatc
    28081 attaacaacc atcgatcaaa tcacttaaca acaggcggta agcaacgcga aattctgcta
    28141 ccatccacgc actctttatc tgaataaatg gcagcgacta tgaaatttgt ctcttttaat
    28201 atcaacggcc tgcgcgccag acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg
    28261 gatgtgattg gcctgcagga gacaaaagtt catgacgata tgtttccgct cgaagaggtg
    28321 gcgaagctcg gctacaacgt gttttatcac gggcagaaag gccattatgg cgtggcgctg
    28381 ctgaccaaag agacgccgat tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg
    28441 cagcggcgga ttattatggc ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac
    28501 ggttacttcc cgcagggtga aagccgcgac catccgataa aattcccggc aaaagcgcag
    28561 ttttatcaga atctgcaaaa ctacctggaa accgaactca aacgtgataa tccggtactg
    28621 attatgggcg atatgaatat cagccctaca gatctggata tcggcattgg cgaagaaaac
    28681 cgtaagcgct ggctgcgtac cggtaaatgc tctttcctgc cggaagagcg cgaatggatg
    28741 gacaggctga tgagctgggg ggtggtcgat accttccgcc atgctaatcc gcaaacagca
    28801 gatcgtttct catggtttga ttaccgctca aaaggttttg acgataaccg tggtctgcgc
    28861 atcgacctgc tgctcgccag ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac
    28921 tatgaaattc gcagcatgga aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc
    28981 cgctaattta gcagctctcc tggctcaaac tgggtcagga gaattaacct tgagaaaaat
    29041 caacaaactg tcagtaatga tttgttgcct gccgtccttt gttataccgt cactgcgttt
    29101 ttagttgtct gaccacttct ctattatcaa gtttgatata ggaaactcca cgatgaacgc
    29161 tgagcgtaac tttctgtttg cctgtcttat ttttgcgctg gtcatttacg ctatccacgc
    29221 tttcggttta ttcgatctgc tcaccgattt accccactta cagacactca ttcgccagag
    29281 cggattgttc ggttatagcc tctatattct gttattcatc aatgccccct ctttctgtta
    29341 ccaggaagca tactggtgat cgctggagga atagtttttg gcccgctctt aggacacaac
    29401 tctcattaat tgccgcaacg ctggcctcct cgtgctcatt cctgctggcg cgctggctgg
    29461 gacgtgattt actgctgaaa tacgtgggcc atagccatac cttccaggcc attgaaaaag
    29521 gcattgcgcg taacggtatt gattttctta ttctgacccg cttaatcccg ttgtttcctt
    29581 acaatattca aaattacgct tacggattca ccaccatcgc cttctggcct tataccctta
    29641 tttcggcact cacgaccctg cccggtattg ttatttatac cgtgatggca agcgatctcg
    29701 ccaatgaagg cattacgctg cgctttattt tacaactctg tctggcgggc ctggcgctgt
    29761 ttattctcat ccagctcgca aaactctacg cccgacacaa acatgtggat ctgtctgctt
    29821 cgcgccgcag cccacttact cacccaaaaa atgaaggata gaacgatgtc gcaacattat
    29881 tcagtgtcat ggaaaaaagg actggctgca ctctgcttac tggctgttgc cgggcttagc
    29941 ggctgcgatc aaaaagagaa tgcggcggca aaagtggaat acgacggact ttcgaacagc
    30001 caaccgctgc gcgtcgatgc caataaccat acggtaacca tgctggtgca aattaatggt
    30061 cgtttcctca ccgacgacac tcgtcacggt attgtgttta aagatggctc caacggacat
    30121 aaatcgctgt ttatggctta tgcgcccccg aaagcatttt atgaagccct gaaagaggca
    30181 ggtggtacgc cgggcgaaaa catgacgatg gataataaag aaacgactca tgtcacaggc
    30241 agcaaactgg atatttcggt caactggcaa ggggcggcaa aagcgtattc cttcgatgaa
    30301 gtgattgttg acagtaatgg caagaaactg gacatgcgct ttggcggtaa tttaacggca
    30361 gcagaagaga agaaaacggg ttgcctggtg tgtctggata gctgcccggt cggcatcgtc
    30421 agcaatgcaa catacactta tggtgcggtg gaaaaacacg gcgaagtgaa attcaaaggc
    30481 aatgcctcag ttctcccggc ggataacacg ctggcaacgg ttacctttaa aatcaccgaa
    30541 taaagccaag ataaaggatg atgaagatac aatcgcgtaa aatctggtac taccgtataa
    30601 ccctcatcat cctgttattc gctatgctgc tcgcatgggc gctgcttccc ggcgtccatg
    30661 agtttatcaa tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt atagaacgct
    30721 ttattcagtc ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg attttacagg
    30781 ccattgctgc accgctacct gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg
    30841 cgttctgggg cggcttgctg tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt
    30901 ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg
    30961 acagtatgga cggctttttc actcgctacg gcaaacacac catcctggtc tgtcggttat
    31021 tgccttttgt ccctttcgat ccaatcagct atgctgccgg tctgacttca atacgttttc
    31081 gctcgttttt tatcgccacc gggcttggtc aattaccggc gactattgtt tattcctggg
    31141 caggcagcat gttaacaggc ggtactttct ggtttgtcac cgaactgttt attctgtttg
    31201 ccctgaccgt ggtgattttt atggcgaaga aaatatggct tgagcgccag aagaggaatg
    31261 actgatgggt ttaccgccgc ttagcaaaat tcctttcatt ttacgtccgc aggcgtggct
    31321 gcatcgtcgc cattacggcg aggtgctaag cccgattcgc tggtgggggc ggatcccgtt
    31381 tatcttttat ttggtgtcaa tgtttgttgg gtggctggag cgcaaacgct caccgctcga
    31441 tccggtagtg cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg
    31501 cgtggatatc accagtatga aagtcgccga gcgtaccggc agcaccgata aactgctggc
    31561 agtggctgac tggcggcaaa gcccgctctt tagcgatgaa gaacggctgg cgctggagta
    31621 cgccgaagcc gcaagcgtca caccgccaac agtcgatgat gccctgcgta cccgactggc
    31681 tgcgcatttt gacgctcagg cgctcaccga actgacggca ttgatcggcc tgcaaaacct
    31741 gtcggctcgt tttaattctg ccatggacat tcccgctcag gggctgtgcc gtattccgga
    31801 aaaacgttct taaggaggga agatgcgcca ttgtgggtgg ttgctgggat tgttatcgct
    31861 gttttctttg gcaacacatg ccagtgactg gcaggaaatt aaaaatgagg ccaaagggca
    31921 aaccgtctgg tttaacgcct ggggcggcga taccgcaatt aaccgctatc tcgactgggt
    31981 tagcggcgag atgaaaaccc attacgctat aaacctgaag attgttcgtc tcgcggatgc
    32041 cgcagacgcg gtgaagcgca ttcagaccga agctgctgcc ggtcgtaaaa cgggcggctc
    32101 ggtggatctg ctctgggtga acggcgaaaa cttccgcacc ttaaa
//
LOCUS       DAEEDS010000043        32129 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEEDS010000043 DAEEDS010000000
VERSION     DAEEDS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32129
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.043"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KLH32_002181"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KLH32_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBE0138996.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="KLH32_002182"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="KLH32_002182"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBE0138997.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="KLH32_002183"
     CDS             complement(3736..4092)
                     /locus_tag="KLH32_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE0138998.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="KLH32_002184"
     CDS             complement(4096..4512)
                     /locus_tag="KLH32_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBE0138999.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KLH32_002185"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KLH32_002185"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBE0139000.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="KLH32_002186"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="KLH32_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KLH32_002187"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KLH32_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBE0139001.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KLH32_002188"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KLH32_002188"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBE0139002.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KLH32_002189"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KLH32_002189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE0139003.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="KLH32_002190"
     CDS             10521..11504
                     /locus_tag="KLH32_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBE0139004.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KLH32_002191"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KLH32_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBE0139005.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KLH32_002192"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KLH32_002192"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBE0139006.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="KLH32_002193"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="KLH32_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="KLH32_002194"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="KLH32_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBE0139007.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="KLH32_002195"
     CDS             complement(15306..15515)
                     /locus_tag="KLH32_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBE0139008.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KLH32_002196"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KLH32_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBE0139009.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KLH32_002197"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KLH32_002197"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBE0139010.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KLH32_002198"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KLH32_002198"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBE0139011.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="KLH32_002199"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="KLH32_002199"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBE0139012.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KLH32_002200"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KLH32_002200"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBE0139013.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KLH32_002201"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KLH32_002201"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBE0139014.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="KLH32_002202"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="KLH32_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="KLH32_002203"
                     /pseudo
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="KLH32_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="KLH32_002204"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="KLH32_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBE0139015.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="KLH32_002205"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="KLH32_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBE0139016.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="KLH32_002206"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="KLH32_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBE0139017.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="KLH32_002207"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="KLH32_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBE0139018.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="KLH32_002208"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="KLH32_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBE0139019.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8172 a         8246 c         7782 g         7929 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtctagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gcctttttt
//
LOCUS       DAEEDS010000044        31768 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEEDS010000044 DAEEDS010000000
VERSION     DAEEDS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31768
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.044"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="KLH32_002209"
     CDS             complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="KLH32_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBE0139020.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQ"
     gene            1796..2542
                     /gene="mipA"
                     /locus_tag="KLH32_002210"
     CDS             1796..2542
                     /gene="mipA"
                     /locus_tag="KLH32_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBE0139021.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2632..3393
                     /locus_tag="KLH32_002211"
     CDS             2632..3393
                     /locus_tag="KLH32_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE0139022.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3444..4328)
                     /locus_tag="KLH32_002212"
     CDS             complement(3444..4328)
                     /locus_tag="KLH32_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBE0139023.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="KLH32_002213"
     CDS             complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="KLH32_002213"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE0139024.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5749..6162
                     /gene="msrB"
                     /locus_tag="KLH32_002214"
     CDS             5749..6162
                     /gene="msrB"
                     /locus_tag="KLH32_002214"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBE0139025.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6204..6476
                     /locus_tag="KLH32_002215"
     CDS             6204..6476
                     /locus_tag="KLH32_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBE0139026.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6846..7922
                     /locus_tag="KLH32_002216"
     CDS             6846..7922
                     /locus_tag="KLH32_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0139027.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7949..9328
                     /locus_tag="KLH32_002217"
     CDS             7949..9328
                     /locus_tag="KLH32_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139028.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9345..10390
                     /locus_tag="KLH32_002218"
                     /pseudo
     CDS             9345..10390
                     /locus_tag="KLH32_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10411..11247
                     /locus_tag="KLH32_002219"
     CDS             10411..11247
                     /locus_tag="KLH32_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBE0139029.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11252..12199
                     /locus_tag="KLH32_002220"
     CDS             11252..12199
                     /locus_tag="KLH32_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBE0139030.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNEALDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12209..13189
                     /gene="ydjG"
                     /locus_tag="KLH32_002221"
     CDS             12209..13189
                     /gene="ydjG"
                     /locus_tag="KLH32_002221"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBE0139031.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13326..14084
                     /locus_tag="KLH32_002222"
     CDS             13326..14084
                     /locus_tag="KLH32_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0139032.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14202..15560
                     /locus_tag="KLH32_002223"
     CDS             14202..15560
                     /locus_tag="KLH32_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139033.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="KLH32_002224"
     CDS             complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="KLH32_002224"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBE0139034.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="KLH32_002225"
     CDS             complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="KLH32_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBE0139035.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="KLH32_002226"
     CDS             complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="KLH32_002226"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBE0139036.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19505..20056
                     /locus_tag="KLH32_002227"
     CDS             19505..20056
                     /locus_tag="KLH32_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBE0139037.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20173..21216
                     /gene="selD"
                     /locus_tag="KLH32_002228"
     CDS             20173..21216
                     /gene="selD"
                     /locus_tag="KLH32_002228"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBE0139038.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVHARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21221..23182
                     /gene="topB"
                     /locus_tag="KLH32_002229"
     CDS             21221..23182
                     /gene="topB"
                     /locus_tag="KLH32_002229"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE0139039.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23309..24349
                     /locus_tag="KLH32_002230"
     CDS             23309..24349
                     /locus_tag="KLH32_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0139040.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24466..25809)
                     /gene="gdhA"
                     /locus_tag="KLH32_002231"
     CDS             complement(24466..25809)
                     /gene="gdhA"
                     /locus_tag="KLH32_002231"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBE0139041.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26045..26317
                     /locus_tag="KLH32_002232"
     CDS             26045..26317
                     /locus_tag="KLH32_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBE0139042.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26283..26690)
                     /gene="nudG"
                     /locus_tag="KLH32_002233"
     CDS             complement(26283..26690)
                     /gene="nudG"
                     /locus_tag="KLH32_002233"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBE0139043.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26777..27397
                     /locus_tag="KLH32_002234"
     CDS             26777..27397
                     /locus_tag="KLH32_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE0139044.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27406..28713)
                     /gene="ynjE"
                     /locus_tag="KLH32_002235"
                     /pseudo
     CDS             complement(27406..28713)
                     /gene="ynjE"
                     /locus_tag="KLH32_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28780..29433)
                     /locus_tag="KLH32_002236"
     CDS             complement(28780..29433)
                     /locus_tag="KLH32_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE0139045.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29433..30950)
                     /locus_tag="KLH32_002237"
                     /pseudo
     CDS             complement(29433..30950)
                     /locus_tag="KLH32_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30923..31768)
                     /locus_tag="KLH32_002238"
                     /pseudo
     CDS             complement(30923..31768)
                     /locus_tag="KLH32_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7957 a         7606 c         8240 g         7965 t
ORIGIN      
        1 tctgctgttc caggacgtag aacagatgga ccgggtttgc cgccacttct acatgatcga
       61 agttgaacac gcgggagagg atcttaaacg caaaacgcgt cgacagaccg ttcatccctt
      121 catcgacacc ggcgtagtca cgatattcct gatacgactt ggctttcgga tcggtgtctt
      181 tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt tctggctctt
      241 tcaggcgcga aagaatggaa aaacgtgaca gtgtttcgag cgtgccaggg gcgcatgggg
      301 cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct tcggaaatgc
      361 gcaagcaata cggcaccttc acgatgtaaa cacggtcgag gaaggcttcg ttgtttttgt
      421 tattacggaa agtgacccat tcggacttgt tcgagtgggc gagaataatc ccgttgaacg
      481 gcagggcgga gataccttcc gtcccgttgt agttaccttc ctgagtggcg gttaacaagg
      541 gatgcagcac ttttatcggt gctttaaaca tctcaacgaa ttccatgatc ccctgattgg
      601 cgcggcacag cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg tagtgttcga
      661 gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg
      721 gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc actacccgga
      781 acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccatggc gacatgatgg
      841 tgccgagata acggcgagga atgccatact ctttttccag aatctgagca tcttcctgcg
      901 gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg gcgctcaata
      961 cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat gatttacccc
     1021 cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc tgagccgcgt
     1081 gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac tcttcaaatg
     1141 ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggg tcctgggctg
     1201 tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg ttggcataag
     1261 cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaactct tcgtccttgg
     1321 cagcttcata tcgctggcga tagtgatcaa atatattcat ggtatgccgt cctttcgttt
     1381 tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag aggtaaactg
     1441 aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg atgagaacaa
     1501 cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctgca
     1561 cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg
     1621 taaagaccag gctcgccagt aacgctaaat ccatttggct gtaagcgcgg tgtcatccgc
     1681 ggcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg
     1741 tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac
     1801 caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc
     1861 tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gagcacccat ataaagatta
     1921 cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg
     1981 tggcttaggt ggtggttact acctgtggaa tgacgcaacg gataaacttt caattaccgc
     2041 ttactggtcg ccgctttact tcaaagcgaa agacagtggc gatcaccaaa tgcgtcacct
     2101 ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg
     2161 ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca ttgtctggga
     2221 tatggcctgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg gtattggtgt
     2281 gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc
     2341 tcgcagcggt ctgcgtggct ataacccgaa tgacagctgg agcccttacc ttgagctgag
     2401 cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct acacccgtct
     2461 gtctgatgaa gttactgaca gcccgatggt ggataaatcc tggactggcc tgatttctac
     2521 cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc ataaaaacag
     2581 ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac aatgcaacaa
     2641 aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat
     2701 atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc aggcattgaa
     2761 ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg
     2821 gttggggaag cattaaccgg tctgcgagag aaggtctttc tcgtctctaa agtctatccg
     2881 tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat
     2941 actgactatc tcgatctcta cttattacac tggtctggca gtttcgcttt tgaagagact
     3001 gttgccgcga tggaaaaatt gatcgcccag ggaaaaatcc gtcgctgggg cgtttctaac
     3061 cttggttatg ccgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact
     3121 aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact cccctggtgc
     3181 cagcaacagc agatgccggt gatggcttac agtccattag cccaggccgg gcggttgcgc
     3241 aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgtccaaca acatgcagct
     3301 gcgcttgagg tcgaactttc gtcagcggaa ttagctatgc tggataaggc atatccggca
     3361 ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac
     3421 ccggcacaca acattaacgc aaattaacgt ttggcaacgc gaatggattg cgccagatgt
     3481 gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca
     3541 aaggtcttgt agccatcgtc cggcatgtcg cccatgctaa ctgaaagcgc cggtcccggg
     3601 ttccagccga caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct
     3661 tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg
     3721 aaggtctgaa taccgtcggt cagtacatct tctttcgcgt cattcacttt atcaatgaag
     3781 cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc
     3841 agggcagagg tggtttcaaa ttcgccatgt gattcaagat cgatttcaca ggttttaccc
     3901 acgcggaaat gcgctaacag cgtaaagtcg tgcggccaga attttttcgt ctcttcgctt
     3961 tgtgtcaatt caaaagtcag cgctacgcca tcagcatctt catgatgtga tttcagcgtc
     4021 cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc cggaccaaac
     4081 cacggccagc aaaccggtac gccaccgcga atagcgacgc catttttgaa cggtgtgttg
     4141 ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc
     4201 tgtaatgcaa aagaggcttt tacctgggga tgatcgacca caatgaggtc cagttcatcc
     4261 agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc aaaaattttc
     4321 ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt
     4381 cgctcttttt agatcacagt gtcatctcaa cttatttgga gatgtgagcg atcaggtcca
     4441 gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt
     4501 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
     4561 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
     4621 cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
     4681 ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
     4741 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
     4801 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
     4861 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
     4921 tgataacttt agccagcgga gctaggcagt tggtggtgca ggaagcgttg gaaacgatgt
     4981 cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag
     5041 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
     5101 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac acccacttcg tcccatttca
     5161 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
     5221 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
     5281 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
     5341 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     5401 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     5461 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct
     5521 aagcattacg cgactgactc gcctcacttt tcctttgggc ttgagaccac atggggtcgg
     5581 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     5641 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     5701 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagtaaat ggcgaataaa
     5761 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     5821 catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat
     5881 cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga ttccggctgt
     5941 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     6001 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
     6061 gtctttcccg acgggccgca accaacaggc gaacgttatt gtgttaactc tgcttcttta
     6121 cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
     6181 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
     6241 aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat ggcgttgcgt
     6301 tgaccgagga acaaaaagag aattgcctgc aactggtgat gctgtggcaa gcccgccata
     6361 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
     6421 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
     6481 ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc acgactcgtc
     6541 tcaattatct cgacccgacc tggctaagtc ccagtccgat ctcttgcagt cgcgttcacg
     6601 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcgaa
     6661 tttgctcttt ttgcgtcccg ccttccacca acctgtaaca gacccagcca ttttctgcgt
     6721 aattgtgatc tgagttgtaa aatgcaggtc attatctgtg ttgtgcagat ttaatattga
     6781 cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac acagaggatg
     6841 acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag atgattcatg
     6901 tcccacaacc catttgtggc ccggaagacg tagtgattga aattaaagcc gcggcaatct
     6961 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
     7021 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
     7081 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgt ccggcctgtg
     7141 aacagggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
     7201 ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa attcatcgtc
     7261 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
     7321 tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttcctggt caggatgttg
     7381 tcgtcattgg cactggccca ctcgggctgt tttccgtaca aatggcgcgg attatggggg
     7441 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
     7501 aagaactggg ggcgacggca gtagtaaatg gttctaccga agatatggtg gcgcgctgcc
     7561 agcaaatttg tggcaaagat aatctgggac tggtgattga atgctccggt gccaatatcg
     7621 cactgaaaca agccatcgaa atgcttcgcc cgaatgggga agtggtacgc gttggaatgg
     7681 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaataaa agcatcattg
     7741 ggcatatggc ctatgactcc acctcatggc gtaacgctat ccggctatta gccagcggcg
     7801 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
     7861 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
     7921 aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc attgtggtgc
     7981 acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt
     8041 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
     8101 gttgggctgg acagataatt ccactacagc cacattctca gcaatcacga ccgccggaat
     8161 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
     8221 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caccgaatat
     8281 ggattttctt attgcctgcc gttttgtgat ggacgttggg ctgggagctt tactggttac
     8341 actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg
     8401 ggtttccttt attggcaact ggtcatatcc gctctgctca ctgattgcga tgggactcac
     8461 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttattcctg caatattgtc
     8521 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
     8581 gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
     8641 ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag cgccacaagc
     8701 ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat taggctcttg
     8761 tgtgctgatt accatgaacg ttgtgcagta cacactaatt aactggttgc caacaatatt
     8821 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
     8881 cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
     8941 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac tgcaaaccag
     9001 tatgttgctc ataacgctga ttggtttctt cctgattact ttcgtctata tgtacgtttg
     9061 ctatgcctcg gcagtgtatg tccctgaaat ctggccgaca gaggccaaac tccgtggctc
     9121 cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt
     9181 gctgctcagt agttatggtg taacgggagt ctttattctt ctgggggctg tttcaaatat
     9241 tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctccg ttgaaagttt
     9301 aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa aattcaaaag
     9361 caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacca gtgcctaaag
     9421 aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat gtacatggtt
     9481 ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc ctgggtcatg
     9541 aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa ccgggggatc
     9601 gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat
     9661 ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc ggcgcattaa
     9721 cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat atggacacga
     9781 tggaaggggc gctggtggag cctgccgcag tcgggatgca tgccgcgatg ctggcagatg
     9841 ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg atgacgttgc
     9901 aagcgtgcaa atgcctgggc gcaacggaaa ttgccgtcgt tgatgtactg gaaaaacgtc
     9961 tggcaatggg ggaacagcct ggtgcgacag tggttattaa tggcgcaaaa gaggacacta
    10021 ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc gaaacagcgg
    10081 gttctgcggt caccgttaaa caggcacctt taatctggta atgcgcggcg gtaaaattat
    10141 gattgttggt actgtacccg gcgattcggc aatcaatttc ctcaaaatca atcgcgaagt
    10201 cactatccag acggtattcc gctatgccaa tcgttatccg gtcacgattg aagctatttc
    10261 ttcagggcga ttcgatgtga aatcgatggt gacgcatatt tacgattatc gggatgtaca
    10321 acaggcattt gaagagtcag ttaacaacaa acgcgacatt attaaaggcg ttattaaaat
    10381 tagcgattaa ttaatacggg agaacagaac atgctcgcag atatcagata ttgggaaaac
    10441 gatgccacaa ataaatatta tgcaattgcc cattttaacg tatggaatgc agaaatgttg
    10501 atgggcgtta aagacgcagc cgaagaagcg aaatccccgg ttattatttc ttttagtaca
    10561 ggttttgtcg gtaacacctc atttgaagat ttctctcaca tgatggtatc aatggcacaa
    10621 aaagcaacgg tgccggtaat aactcattgg gatcatggtc ggagtattga gattattcat
    10681 aacgcctgga ctcatggtat gaattcatta atgcgtgatg cttctgcatt tgatttcgaa
    10741 gaaaatattc gtttaaccaa agaggctgtt gatttctttc atccgctggg tattccggta
    10801 gaggcggaat tagggcatgt cggtaatgaa accgtttatg aggaagcgct ggcgggctat
    10861 cactataccg atcctgacca ggcggctgaa tttgttgaac gtacgggctg tgactcattg
    10921 gctgtcgcca tcggcaacca gcatggggtt tatacgtcag agccgcaatt gaactttgag
    10981 gtcgtcaaac gcgtacgcga tgctgtttct gttccgctgg ttttgcatgg cgcatcgggg
    11041 atcagtgatg ccgacattaa aactgcaatt tcgttgggta ttgcgaaaat caacattcat
    11101 acggagctct gtcaggccgc aatggtagca gtgaaagaaa accaggatca gcctttcctg
    11161 catttagaac gtgaagtacg taaagctgta aaagaacggg cattggaaaa aattgaactg
    11221 ttcggttctg atggcaaagc ggaataatga tatggataat ctcgacgtta tttgtatagg
    11281 tgccgctatt gttgatattc cattacaacc ggtcagtaaa aatatctttg atgtagattc
    11341 ttatcctctt gaaagaatcg caatgagcac cgggggcgat gcaataaatg aagcaacaat
    11401 tatttctcgt ctggggcatc gtaccgcatt aatgagccgc attggtaaag atgcctcagg
    11461 gcaatttatt ctcgaccatt gtcgcaaaga gaatattgat attcaaagcc tgaaacagga
    11521 cgtcagcata gatacatcta ttaacgttgg actggtgacg gaggatggcg agcggacgtt
    11581 tgtcacgaac cgtaacggca gtttgtggaa attaaatatt gacgatgttg attttgcgcg
    11641 gtttcctcag gcgaaattat tatcactggc cagtattttc aacagtcctc tattggatgg
    11701 taaagcgcta acagaaattt ttacgcaagc gaaagcccgg cagatgatca tttgtgccga
    11761 tatgatcaaa ccgcggttga atgaagcgct ggatgatatt tgcgaagcat tgagttatgt
    11821 cgattatctg tttccaaatt ttgccgagga aaaattactc acagggaaag agacactgga
    11881 tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa
    11941 agacggctgc tttatcaagc gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat
    12001 aaccgccatc gacaccattg gcgcgggcga taactttgct tcaggtttta ttgcggcact
    12061 gttagaaggc aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc
    12121 ggttctaagc gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg aacaattgct
    12181 ggaagaatac gaaggataat gaaggcaaat gaaaaagata cctttaggca cgacggatat
    12241 aacgctttcg cgaatggggt tggggacatg ggccattggc ggcggtcctg catggaatgg
    12301 cgatctcgat cggcaaatat gtattgatac gattcttgaa gcccatcgtt gtggcattaa
    12361 tctgattgat actgcgctag gatataactt tggcaatagt gaagttatcg tcggtcaggc
    12421 gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc aaatgcggca ttgtctggga
    12481 acgaaaagga agtttattca acaaagttgg cgatcggcag ttgtataaaa acctttcccc
    12541 ggaatctatc cgcgaagagg tagaagccag cttgcaacgt ctgggtattg attacatcga
    12601 tatctatatg acgcactggc agtcggtgcc gccatttttt acgccgatag ctgaaactgt
    12661 cgcagtgctt aatgagttaa aggctgaagg gaaaattcgc gctataggcg ctgctaacgt
    12721 cgatgctgac catatccgcg agtatctgca atatggtgaa ctggatatta ttcaggcgaa
    12781 atacagtatt ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc gtgataatgg
    12841 cattgtggtt caggtttatt ccccgctaga gcagggattg ttgaccggca ccatcactcg
    12901 tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct
    12961 gaaagtgatt gatatgcttg aacagtggca gccactttgt gctcgttatc agtgcacaat
    13021 tcccactctg gcactggcgt ggatattaaa acagagtgat ttaatctcca ttcttagtgg
    13081 ggctactgca ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga
    13141 tgcagacgca acattgatga gggaaatggc agaggccctg gagcgttaaa tactcaacac
    13201 gattcacctc tgtttctgtg aaaactgtga tgaaaatctg tgattcaaat aggttatttt
    13261 gaaagtaaac atcggttatg cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg
    13321 taatcgtggc ggcaaaagac aggattcagg caattaagca aatggttgcc aacgataaaa
    13381 aggtgacagt ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc attcgccgcg
    13441 atcttgagaa gctggaagat gaaggctttt tgaccagaac ctatggtggt gctgttttaa
    13501 atacagcggt gttgacggag aatatccatt tttataagcg cgcttcatcg ttttatgaag
    13561 agaagctgct tattgcacgc aaggcactac cctttatcga caataaaacc accatggcag
    13621 ccgattccag tagtaccgta atggaattgc tcaaattgtt acaggaccgt agtgacctga
    13681 cgttgctaac caactcggcg gaagcgattc atgttctggc tcagtcagaa attaaagtcg
    13741 tttcaacggg tggggaacta aacaaaaata cactttccct gcaaggaaga attactaaag
    13801 agatcatcag ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt cttgatatta
    13861 acagcggcgc gctggactct aatgaagcgg aagctgaaat caaaaagaca atgatccgtc
    13921 aggcgacaga agttgcgtta ttggttgatc actctaagtt tgatcgtaaa gcttttgtcc
    13981 agttagctga ctttagtcat attaattata taataactga taaatcaccg ggtgcagagt
    14041 ggatagcatt ttgcaaagac aataatatcc agctggtgtg gtaatttaag cacacgttgt
    14101 caatattatt tgttcctgcg actggaaata aaatgatgat aaagatgcgg caagtgaatt
    14161 tgccgcaatt gctacctttt atagcaaatc cgaggcaccc aatggaacaa tatgatcaaa
    14221 ttggcgcaag actggaccgc ttgcctttgg cccggtttca ttatcgtata tttggtataa
    14281 taagctttag tctgttatta acggggtttt tgagttactc aggtaatgtc gtcttagcaa
    14341 agctggtaag caatggatgg tcaaataatt tcctctatgc cgcctttacc tcggcattaa
    14401 tgtttggtta tttcatcggc tcacttactg gtgggtttat tggtgactac tttgggcggc
    14461 gcagggcgtt tcgcataaat cttctcatcg tcggtattgc tgcaacaggg gccgcttttg
    14521 tccctgatat gtactggctc attttctttc gcttcctgat gggaacagga atgggggcgc
    14581 tgattatggt tggctatgcc tcatttacgg agtttatccc cgcgacggtg cgtggaaaat
    14641 ggtccgcgcg gctctcattt gttggtaact ggtcgcccat gctgtctgcg gcgataggcg
    14701 tggtggttat cgcttttttt agttggcgaa taatgtttct tctgggtggt attggcatac
    14761 tgttagcctg gtttctctca ggtaaatact ttatcgagtc gccacgatgg ctggaaggga
    14821 aagggcaaat cgccggtgca gaaagccaac ttcgtgaagt agagcagcaa attgaaaggg
    14881 agaagagtat tcgtttaccc cagcttactt tgaaccagag caacagcaag gttaaggtaa
    14941 tcaagggtac cttcaggctc ctgtttaaag gggaaatgtt acgacgtaca ttagttgcga
    15001 taactgtttt aattgcaatg aacatttcgc tttataccat taccgtatgg ataccgacca
    15061 tatttgttaa ctccggcatt gatgtcgata aatcaatatt aatgaccgct gttattatga
    15121 ttggtgctcc ggtaggaata tttattgcag cattaattat tgatcatttt cctcgtcgat
    15181 tatttggctc cgccttactt attattattg ccgtgttagg ctatatctat tcaagtcaga
    15241 ctacagagtg ggcgatttta atctatggtc tggtgatgat cttcttttta tacatgtatg
    15301 tttgcttcgc gtcggcggtt tatatcccgg agctttggcc aacgcattta cgcctgcgcg
    15361 gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt tttcacgccc tatggcgttg
    15421 cggcattatt aacacattat gggtcgatca cggtgtttat ggtgcttggt gtcatgttag
    15481 tgctctgtgc gctggttctc tccatttttg gcatcgaaac gcggaaggtg tcgctggaag
    15541 agatttctga ggtgaattaa atctttcgtt gatggcatca attgccagat gtaccactga
    15601 cgtgttttat ttggcctaca atgccagtac agaattgtag gccagcgtaa aattacccct
    15661 gcgtctcttc ccagtctgcc agcgtatata gcgttgcccc agctgctgac atctccataa
    15721 acgcgtgtgc actgtcctgg ggctggatat tcacgccacg acagccatcg gtaatcacgt
    15781 ttaccttata acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag
    15841 ccaggcccat aacgatcaat tcatcgattt catgatcgcg caaccagtca tcaagcgcgg
    15901 ttttctgccg acgtccgtta tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc
    15961 ctttatggaa caccgctgcg atctcttttt gattcagtaa cggatgtaat tgtgcgcctt
    16021 cactgttctg cacacagtgt tcaggccaga aagtttgccg caggccgccg agttggcctg
    16081 gcgtataagg ctctacgccg tgctggctgg caaagctgcc gtgattcgcc ggatgccagt
    16141 cctggctggc gataaccgct tctccgcgcg actggcacca gtcaataagg cggttagcaa
    16201 cctccaccgt actgtcacct tccggtacgg cgagcgcgcc accagcacag aaatcatttt
    16261 gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt ctccttaatc atccggcgtc
    16321 agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc cagttcctgg
    16381 ctcagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc accgtcaata
    16441 acgccggcat gggcgagggc gttaccggtg gcataaccac tcatattcac tttaccggac
    16501 atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg taattcctgc
    16561 aggaaggctt tgttttgtgg cgcattaccc acgccatagg agcgcagaat caatgctttc
    16621 accggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccctggata aatcgtcacc
    16681 acgccaattg gttgtggcgt gattggatga acgattaatt ccccttcacc gtgcggggcg
    16741 ggtggcgtat tcaaacgacg aatatggata ccagcttcca gtaacggagg aaggtttgga
    16801 gaggcaaacg catcaaaacc atcggcatgg gctttggtag tgcggttgcc gcgatacaat
    16861 cggttattga aaaagagcgt tacttcgttg atcggataat tcgccgcaac gtacaacgca
    16921 ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg tgaccctgtc
    16981 acaatgaccg gtttaccgag attctcgagc atgaacgaca gcgcagaggc ggtatacgcc
    17041 atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt cgtcatagtg cgctttaata
    17101 tcttcagcaa tatgctgcca gtcttccggc gtcatatcag aagaatccat caacggcgta
    17161 tattcatgaa tggtgaaatc tggcatctcc gggcgatgga attccggcat cagcgccagt
    17221 tggcgttgta gatgacctga caccggaata taaccctgct cggaacgctg catcccgatg
    17281 gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat atgaatgata
    17341 tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc acctgatact atgcggataa
    17401 ggaaatatgg ggcgagacag cgggaagagg attcgtcgag gtaaaaaata atgtggccga
    17461 cgatctgtcg gccactcagt acaagactta acgcacgttg gcgcaggtca ggcaaaacgc
    17521 ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt taacggtaga
    17581 ggctaccgag gcaagcggtg caggtaacat ggcctggaac gcatctggca acattgcccg
    17641 gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat caacgtagta
    17701 ttccagatgc cactgtttca cttttgcgag ctctgctgct ttggcaaccg catcatcgaa
    17761 atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc
    17821 ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc gcatcagcaa ccagcgtgat
    17881 aaagcgttta tagccattct caatgcttaa ttgcatcatc tgctgcgctt ccggcggcag
    17941 tgccctggtg atagaaacat ccgccagcgg tgaagttgag acaccatcag tatgaacacc
    18001 aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga tagaaccggt
    18061 cagggtgctg gggttagcca caatgtaatt agctggcgtg gaaatccagt aaccaccaga
    18121 tgccgccatg ccgcccatcg atacaaccac agacttaccc gctgcccggg ctgctgccag
    18181 ttcagcgcga atcacttcag acgcggtaac gctgccgcct gggctattaa cacgcaggac
    18241 aatcgctttc actttcgggt caaggcgagc gtcgcggatt tgtgccgcag tggtatcacc
    18301 gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga agacgacacc
    18361 gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg
    18421 ataattttta tcagtcttac tccagccgaa ctctttggtc agagcttttt cgatttcggc
    18481 actcgatgcc agtgcatcga ccagcttgtt ttccagtgca tatttcgcgg tatcgccacc
    18541 ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct gagcagggat
    18601 ctgccggtta gcggcaacag tattcagata gttttgccac agctcaccaa tccagcggct
    18661 gtcagcttcg cgggctgccg gtgacatatc atcacgaata aacggttcaa cggcagattt
    18721 atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagca acgatttgta
    18781 gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg ccttgcggtg acagccaaat
    18841 tttattggcg aaactggcga gataatattg cccctggctg tagttctcgc caacggcata
    18901 aaccggtttc ccgctgtcac gaaactcttt cagggctttg ccgatgtact gcatagacgg
    18961 ctggtcgccg cctgcgaagt ttttcagatc catcacgata ccggtgatat tgcggtcgtc
    19021 cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgca gacgatcgga
    19081 actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt ctggtttatc
    19141 gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt ctttcgaatc
    19201 accaccactg acctgcatcc agatccccac accaaccagt acgaggaaaa taaagaacag
    19261 gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa aaaatccggc
    19321 aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc tgtcacaggc
    19381 gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa ttgcggactg
    19441 tgctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcacga caggagttaa
    19501 tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgcc tggctgaacc
    19561 cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca
    19621 taagtccatg caaccgtggc atttttttgt gattgaaggg gaagggtgcg agcgtttcag
    19681 cgccgtactg gaacaggggg cgattgctgc cggtagtgat gataaagcta tcgacaaagc
    19741 ccgtaatgcg ccgttccgcg caccgctcat catcacggtg gtggcgaaat gcaaagaaaa
    19801 tcataaagtc ccgcgatggg aacaggaaat gtctgccgga tgcgcggtca tggcgatgca
    19861 aatggcagcc gttgcccagg gatttggcgg catctggcgc agtggcgcat taactgaaag
    19921 tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg gttttctcta
    19981 cctcggcacg ccgcagttga aagcatctac gtcgattaac gtcccggacc cgacgccgtt
    20041 tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa
    20101 ttcagacact ctcacttatc acttcacgga atgagggtta ccatacccgg attgcattga
    20161 caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca cggagctggt
    20221 tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag
    20281 tttgttgatc tgaatttgct tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat
    20341 ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat cgttgataat
    20401 cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt cgcgatgggg
    20461 ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact ttccccggaa
    20521 attgcccgcg aagtaaccga aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg
    20581 gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt aacggggatc
    20641 gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa actgttcctg
    20701 acgaaaccgc tggggatcgg cgttcttacc acggctgaga aaaaatcact gttgaaacca
    20761 gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg cgcgtccttt
    20821 gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gctttggtct gctgggccac
    20881 ttgagcgaaa tgtgtcaggg ggctggtgtg catgcacgcg tcgactatga agcgatcccg
    20941 aaactccccg gtgttgaaga gtacattaag ttgggcgcag tacctggcgg cactgaacgt
    21001 aactttgcca gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg cgatctgctg
    21061 tgcgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc agaaaatgag
    21121 gttaaaacta cagccgccga gtttggcatt gaactgacgg cgattggcga actggtgcca
    21181 gcgcgcggcg gtcgtgccat ggttgagatc cgttaattta atgcggttgt ttattgccga
    21241 aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc aaaccgcacc ggaaaggcga
    21301 tggctttatc gagtgcggta atggtcaggt ggtgacttgg tgtatcggtc acctgcttga
    21361 gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg cggatttgcc
    21421 gattgtcccg gaaaagtggc aattacagcc acgaccctcc gtgaccaaac aacttaacgt
    21481 catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggggacc cggatcgtga
    21541 agggcaattg ctggtggatg aagtgctgga ctatctgcaa ctggcaccgg aaaagcgcca
    21601 gcaggtgcag cgttgcttga taaacgacct gaacccgcag gcggttgagc gggcgatcga
    21661 ccgtcttcgt tccaacagtg agtttgtgcc attgtgcgtt tctgcgctgg cgcgagcgcg
    21721 tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat accattctcg gtcgcaatgc
    21781 cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca cccgtgctcg ggctggtggt
    21841 gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca
    21901 tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg aagcgtgtga
    21961 accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa
    22021 ccgcattagt ggtcaaccgg ctattgtcac cagctataac gataaacggg aatcagaatc
    22081 cgcgccgctg ccgttttcgc tttcggcgtt gcagattgaa gcggcaaaac gcttcggtct
    22141 gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgcaca agctaatcac
    22201 ttatccgcgt tccgattgtc gctatttgcc agaagaacat tttgccggac gccacgcggt
    22261 gatgaatgcc atcagcgttc atgcaccgga tctgttgccg cagccagtgg tagatccaga
    22321 tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca tcatcccgac
    22381 cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata acctgattgc
    22441 ccgtcaatac ctgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaact
    22501 ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag caggctggcg
    22561 cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccat tgcctgtggt
    22621 ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg gtagagcggc aaacccagcc
    22681 gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg cgcgctttgt
    22741 gcaggacaaa gatctgaaaa aggtccttcg tgcgaccgat ggtctgggga cagaagcaac
    22801 ccgtgccggg attattgaac tgctgttcaa gcgtggtttt ctgaccaaaa aagggcgcta
    22861 tatccactcc accgacgccg gaaaagcgtt attccattcg ctgccagaaa tggcgacgcg
    22921 accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg
    22981 ctatcaggac tttatgcagc cgctggtggg gacgctatat cagcttattg atcaagccaa
    23041 acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg ggcagtggtg gcagtgctga
    23101 taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa agtccgccag cagatgaagc
    23161 cggaagcggg gcgatagcgt aagcgagcga atctttcgtg ctattcgagt catattctga
    23221 aatatccagc ggatcaagaa aattcgttgg atattttttt gcatggataa aattatcgcc
    23281 tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt ttattgcaaa accatcctgg
    23341 gagcgagaag tattctttta atggctgggg aatatttaat agtaattttg aacgggtgat
    23401 taaagaaaat aaggccatgc tgctttgtaa gtgggggttt tatttaacat gtgttgtcgc
    23461 tgtaatgttt gtattcgcag cgataacatc caacggtttg aatgaaagag gcctgattac
    23521 cgcgggatgc tcttttcttt atctattaat tatgatgggg cttattgttc gggccggttt
    23581 taaagcaaaa aaagaacaac tgcattatta tcaggctaaa ggtattgagc cgctcagtat
    23641 cgagaagtta caggcgctac aattgatcgc accttatcga ttctatcata agcaatggtc
    23701 tgaaacgcta gagttctggc cgcgaaagcc tgaacctggc aaagatacct tccaatatca
    23761 tgtgcttcct tttgactcga tcgatatcat cagtaaaaga cgcgagtctt tagagaatca
    23821 atggggtatc gaagatagcg aaagttattg tgccttaatg gagcattttc tttctggcga
    23881 ccatggagcc aataccttta aagcaaacat ggaggaagcc ccagagcagg ttatcgcctt
    23941 gttgaataaa tttgctgttt ttccctcaga ctatatctct gattgcgcta ataatcgctc
    24001 cggtaaatcc tcggcgaagc taatatgggc ggcggaatta tcatggatga tctcgatatc
    24061 aagcacagct tttcaaaacg ggacaattga agaagaactg gcctggcatt atataatgct
    24121 tgcttctcga aaggcgcacg agttgttcga aagcgaagaa gattatcaaa aaaatagtct
    24181 aatgggattt ctttactggc atatctgctg ctatcgcaga aagttaacgg atgctgaact
    24241 tgaagcgtgt tatcgttacg acaagcagtt ttgggagcac tacagtaaga aatgccgttg
    24301 gcccataaga aatgttccct ggggagcatc atccgttaaa tactcataaa cgcctgaaat
    24361 tttgccgggg gcgctgcgct tgaccggcct acattagagc gtaactgcaa tgaattgtgc
    24421 ccatctgtag gcctgataag cgtagcgcca tcaggcattt acaacttaaa tcacaccctg
    24481 cgccagcatc gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt
    24541 ggtttgctca ccttcaccac catgctcaac acaggcatgg tggatatcca gcatgatgtg
    24601 atgcaaacgt gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat
    24661 ttccaggccc gacgttgcta caccgccagc attagccgct ttacccggtg caaacagtac
    24721 gcctgcctgc tggaacagtt cagtcgcttc gatggtggtc ggcatatttg ccccttcagc
    24781 gacggcttta acgccattgg cgataagctg atgcgcggcg tcaacatcca gttcgttctg
    24841 ggtggcgcaa ggcagggcga tatcaaccgg tacagaccac ggctgttggc cttcgagata
    24901 gaccagacca aattctttgg cgtaatctgc cactcgacca tcgcggctgg ctttgatttc
    24961 gataagacgt gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga
    25021 gtctgacgca gtgatcacac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc
    25081 gacgttgccg gagccagaaa cggaaacgct catcccttca aaacccatac cgtggcgttt
    25141 taacattgct tctgtgaaat aaaccagacc gtagccggta gcttccgggc gaataagact
    25201 accgccaaat gaaaggccct taccggtgaa gacgcaggcg gtattgttgg agagcttttt
    25261 catcatcccc gccataaagc cgacttcacg accaccaacg ccgatatcac ctgccggaac
    25321 gtcggtatcc gcgcccaggt ggcgatacag ttcagtcatc agcgtctggc aaaaacgcat
    25381 cactttacct tcgctttttc ctttcggatc gaaatcgctg ccgcctttac caccgcccat
    25441 cggcagggta gtcagggcat ttttgaaggt ttgttcaaag ccgaggaatt tgagaatgga
    25501 aaggttaact gacggatgga agcgcatacc gcctttgtac gggccgatgg cagagctgaa
    25561 ctgcacacgc catgcacggt tgacctgtat ctggttgcga tcatcaaccc ataccacgcg
    25621 aaactggatc acgcgctccg gttcaaccag acgctccagt aatgacatct ggcgatattt
    25681 tggattttgt tcaagaaaag gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa
    25741 ctcggtttga ttcgggtcgc gcttttggac atggttgagg aatgactcca gagaatatgt
    25801 ctgatccata gatataaaaa ccttatatat taatacgatt gtgcttatgt tgtttatgtc
    25861 atgtgctttt gcagttttcg actataccat caagaatgtg acggtaagca agaaaaaaat
    25921 gacccaggaa agcaaattaa taacgagagt aatctcataa ccaaatgcga taactcagac
    25981 gctaaagttt tgccacccgg ctaccgttat cagtagcaga gacaataacg ttaaggagtt
    26041 ccctgtgagt cgagcattgt tcgccgtagt attagcattt ccattaattg cactggctaa
    26101 cccgcattat cgtccggacg tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg
    26161 gcagtcagcg cagccctgta ctcagtgctg cgtttatcag gatcaaaatt attctgaagg
    26221 ggcggtaatt aaagccgaag gaatactgct gcaatgccag cgtgatgaca aaacccttag
    26281 cactaatccg ctgctctggc ggcgcgtaaa gccataaacg cctctaataa tggaatgtca
    26341 gcaggggcca gcggatattg cagcgcctct tcaggtgagc accagaccag cgcctgatgt
    26401 tcatgtgcct gtaacgtccc gtggaagtcg ggtacgtgcc aggcatgaag atggataatc
    26461 cggcccgaaa cttctcgctg atggctggca acatactcac ccacagttgc ttcgatgtcc
    26521 aattcttcgt ttaactcacg caccagcacc tgctgctggc tttcatccag ctcgacttta
    26581 cctccggcaa actcccataa tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa
    26641 attttgccat cacgttcaat gatggcggca acaacttcaa tcattttcat aaattacaca
    26701 cgggcagata agcgaaaaat gccataatat ccgcactgtt gctcaatcag aaaggcttta
    26761 tatttaagga gtgcctgtgc tagaccgcca tcttcatccc cggattaaac cgttgttgca
    26821 tcagtgcgtg cgggttcttg ataaaccggg cattacgcca gatggtttaa cattagtggg
    26881 atttgccatc ggcgtgctgg ctctgccgtt tctggcgctg ggctggtatc tggcggcgct
    26941 agtcgtcatt ttgttgaaca ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag
    27001 agggcttacc gacgcgggcg gctttcttga tatttctctc gattttctct tttacgcgct
    27061 ggtgccgttt ggctttattc ttgctgcacc ggagcaaaat gcgctggcag gcggctggtt
    27121 gttgtttgcg tttatcggca cgggcagcag ttttctcgcc tttgctgcac tggcagcaaa
    27181 acatcagatt gataaccccg gttatgcaca taagtcgttt tactatctgg ggggattaac
    27241 cgaaggcaca gaaacgatct tactgtttgt gctgggatgt ttattccctg catggtttgc
    27301 gtggtttgcg tggatcttcg gtgcgttgtg ctggatgacg acctttacgc gggtgtggag
    27361 tgggtatctg acgctgaagt cgctccagcg tcagtaattg caatgttatt tgctactgcc
    27421 cgggccgcgt tcaccggttg caaccggatt ttttggatcg ctgctccatt cgtaccagcc
    27481 gccgtcatac acggaaacat tcttccaacc catggcgcgt gcgtacataa aggtttcgga
    27541 cgcgcgccag ccggtgccgc agtagaatga aacttgctga tctggtttga tattccatgc
    27601 tttccacata gcggtaatat catcggcgct acgcatggtg ccatccgggt tatagaaatc
    27661 ttccatatgc gtcgagtcgc taccagcgtg tccccaacgt gctccggcta tttcaccttt
    27721 tggtttaata tagctgtaac cgctggtcgt accgataaat tctggccacg aacgaatgct
    27781 caccagcgat gcatcctggc gatgcagcag tccacgggcc tgttccatat cgagcatcaa
    27841 ctgcggttgt gccgggatct tcacgccgaa atccggttcc gctttcactt tcggtggcgt
    27901 tcctcgctca acaggcagtc ccgcgtcgga ccaggtttgc cagccgccat ccagcagacg
    27961 cacatctttc acgcctgcgt agagcataat ttgcgccaca cgcgctccag cgtatacgtc
    28021 acgcccgtac agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca gcatcgcttt
    28081 tagttgttca tcagaaactt tgttccacag cggttcgctt tccacttcgt tggtatcgat
    28141 gtagtcagcg ccaggaatat ggctgataag gtaaagctta ggagcgccct aggccgcttc
    28201 aatgactttc cagtcaccga caggtttcgc cgtaacctct ttaccttgtt gcaggtcatg
    28261 cagccattgc ggataaacca gctgctcaaa gtgcggcagt ttttgtagac gggaaggttc
    28321 gcttagcgcg tcactcagaa tggagatatg cgtaaaacct gctttttgca gtcgcgtttt
    28381 gacggcgtcg acatctttgc cattaccgta cagcgccacc ggggcatcgg ctttcaggtt
    28441 atgttgcttg atccacgcgt tgagctgttc ggtgctcatt ttgtcgagcc agctagcaga
    28501 gaggtttaag gcggcaggtt catgaccaga agggccattt aaggtttgtg gccagccgtt
    28561 ataaaacgcg ctggggcgag tatctatcgc tttgccattt tgttgctgaa gttggtcaag
    28621 tgtaagaggc ttcgccagtt cagcggccca cgaagaagcg caagccagcc ctaaagccat
    28681 tgccagcgcg gtcatttgag aaacacgttt catcgaaaat cccgacattg tagttttgag
    28741 tgcgccattc tgaactgacg cattgagaaa aactttgcgt tatcgcagtt tgttgtaatt
    28801 ttctgaccac tgcgccatat ccagaacaga actatcagca ggaacatcct ggagatcgtg
    28861 cgttacctga acgacgggga tcgccagggc gcgaacttcg ctgaacaccc actggcgaaa
    28921 attatcgcgc agagccacat caagacggct gaatggctca tccaggagta acgcttttgg
    28981 ctgggcgaga agggcgcgta gtagagcaac gcgcgctcgc tgaccgccag acaaagtggc
    29041 aggatcctga tggaaagctc cgtcaaggcc tgaacgctca agtgcatcat tcacggcatt
    29101 acgtcgggca ttccccttaa gtgtcgccgg tagcgccagc agtaaatttt gcccgacgct
    29161 gaactggtca aataacagtg catcctgaaa aagaatgcca atctgacgct gtgcggtggg
    29221 tagcatgtca atccgttgct cattgagcca tagctcacct gtacaagaaa actgtccggc
    29281 cagtgcacca atcatccatg aaaacagagt ggattttcca cagccagacg gccccattaa
    29341 cgtgacgatg tcacctttat caaccgtaaa gttaacgttt gtcagcaagc ggctttctgg
    29401 taaacgtagc gaaacatttt tcacgcagag cattagcgga ttccttgtct gacataacct
    29461 acccattttg cgactaacgc ggtcagggca aaaataataa gcggtaacaa cagttgccat
    29521 aacgcctggg cggcgagaat accgttgctg ccgccgctgc ttaatgccac cgcctcagtg
    29581 gtgagcgtcg gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg cgcaatactg
    29641 actgaaaatc ccaccgcgaa ggcagtcagc acagggcgca acatgagtgg gcatttcacg
    29701 taaaagaaga ttttggcccg cgaccagccc agtgtttgcg caatcaaaat taaccgtgaa
    29761 tcaatgcgct gccaggcggg ttgcaggata aacagcatcc acggcattac ccacagcaga
    29821 tgcccccaga ccaccgccgt ccagcttcca tccaggttca gccatagcgc cagcgtgtac
    29881 tggcccgcca ccagtggcag agcgggcaat agaatgggca accatagcca caactggcga
    29941 cgctgtgccc cccattccag ccacagcagt aacaggagca aagcgatgaa tgttgctacc
    30001 agccccattg tcagactgtt gatgagtgct tcactattga tcgtcgactg atccgcgagg
    30061 atcgccagca gaaccacaca cagcacaccg gttaagggta aaaaactcgc cagcgtagtg
    30121 cctggtaata aaggtgtggc aggcttgcga atgccatcta cgcggggaat agtgcgccgc
    30181 cagctgcgcc aaaacgtagg cggcgagtaa cagcatcagc agcagactgg cgagtgcgcc
    30241 tttcgtttgt tgatccgcgt cgccctgagt taaccactgc cagctaatta ctgccagcgt
    30301 cggtggatta cctggcccaa gaataatcgc cacatccacg accgaaagcg accaggcaac
    30361 aatcgccagc atcgccattg ccagcgaagg cgcgacggag ggcaacagca gccagttcag
    30421 gcattgccag cggctgtagc ccagcgaatc cagcacaatg acctgctgca acagccgttt
    30481 ttcgctcaat actgccacta agatccacag cagaaatgcg ctttctttca ctgcaagggt
    30541 gaggcccaga ccgatccaaa tcggtccatt ggcggagtga aatacgggaa atagtcatat
    30601 agcagccctc cgtcagcaaa gagcagcagg gcgctggtgg caaaagccac atggggaatg
    30661 gcgagcagcc acggcagacg ggcgcacata cgctgccatt tcggcccagg ccatagcgcc
    30721 acaatcacca gcagggcaat caacaatgcc ccgaccgccg cgatggttgt cgacaccagc
    30781 gttgccagta atgcctgcgg taactgtgga tcggcaaata acgcctgcca gcgtgtcaac
    30841 gacaacgcag gcgatatcag cgtgagagct gccgggatca gcggtgcata aatcaccgcc
    30901 accatcgccc acagcaaaaa gattaatgcg taccgtaacg gcgtaaccat tcttgttcca
    30961 gtgcattcac ccatcctgcg tgcggttcag ccagtaccgg cggcagatcc tgcggcattc
    31021 ttgattgcaa tatttcgcgc tgcccgtcag gcagtttttg cggatcgaga acagaaggat
    31081 cgccccagac aacgggatct gctttacgca gttgcgcatc gggtgagagc aggaaattgg
    31141 caactacctt cgccgcagca ctggcattcg cgttggctgg agtggtgacg aaatgcacgt
    31201 tgccaatcat cccctcgcga aagccaaaac tgtaactgct tgccggcaaa tcgccgctgg
    31261 cgattttttg ctgcgcatgt gcggggttaa aggtcagcga caggcgcaat gtgccggctt
    31321 tcagcagagc atccatccgc gcgggcgaag gcgggaaatc ctttccttcg cgccacaaat
    31381 acggatgcag tgcatcaaga tattgctaca agggcgcagt gacacgggcg aaagtcgcat
    31441 cgtccggcgc ttcttttaat gcggcgggat ctggcgtcag cataatcagc aactgttcaa
    31501 gaaacgccgt gccggtaaag tccggtgggc gcggataagt aaccgtgccg ggattggctt
    31561 tagcaaactc cagtaaggct tgcggcgttt gtggtggctg tggcgtaaca tcgcggcggg
    31621 cgataaacgt cagttgtgcg ccgccccagg gcgattccgc accttgtgtc ggcactgaaa
    31681 aatctccccg caccggcagc tgtgtgtcga catagcgcca gttgggcaga gtctccgccc
    31741 agcccgtttg taataaattg gcctcttt
//
LOCUS       DAEEDS010000045        29802 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEEDS010000045 DAEEDS010000000
VERSION     DAEEDS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29802
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.045"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            58..852
                     /locus_tag="KLH32_002239"
     CDS             58..852
                     /locus_tag="KLH32_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE0139046.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            874..2283
                     /locus_tag="KLH32_002240"
     CDS             874..2283
                     /locus_tag="KLH32_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139047.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="KLH32_002241"
     CDS             2261..3040
                     /locus_tag="KLH32_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBE0139048.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="KLH32_002242"
     CDS             3037..3897
                     /locus_tag="KLH32_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBE0139049.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="KLH32_002243"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="KLH32_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="KLH32_002244"
     CDS             complement(4637..4897)
                     /locus_tag="KLH32_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBE0139050.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="KLH32_002245"
     CDS             complement(4888..6159)
                     /locus_tag="KLH32_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0139051.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KLH32_002246"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KLH32_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBE0139052.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="KLH32_002247"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="KLH32_002247"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBE0139053.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="KLH32_002248"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="KLH32_002248"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBE0139054.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="KLH32_002249"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="KLH32_002249"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBE0139055.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="KLH32_002250"
     CDS             11501..11653
                     /locus_tag="KLH32_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE0139056.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11847..12938
                     /locus_tag="KLH32_002251"
                     /pseudo
     CDS             11847..12938
                     /locus_tag="KLH32_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13084..14121)
                     /gene="iap"
                     /locus_tag="KLH32_002252"
     CDS             complement(13084..14121)
                     /gene="iap"
                     /locus_tag="KLH32_002252"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBE0139057.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14373..15281
                     /gene="cysD"
                     /locus_tag="KLH32_002253"
     CDS             14373..15281
                     /gene="cysD"
                     /locus_tag="KLH32_002253"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBE0139058.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15283..16710
                     /gene="cysN"
                     /locus_tag="KLH32_002254"
     CDS             15283..16710
                     /gene="cysN"
                     /locus_tag="KLH32_002254"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBE0139059.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16710..17315
                     /gene="cysC"
                     /locus_tag="KLH32_002255"
     CDS             16710..17315
                     /gene="cysC"
                     /locus_tag="KLH32_002255"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBE0139060.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17365..17688
                     /locus_tag="KLH32_002256"
     CDS             17365..17688
                     /locus_tag="KLH32_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBE0139061.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17882..18193
                     /gene="ftsB"
                     /locus_tag="KLH32_002257"
     CDS             17882..18193
                     /gene="ftsB"
                     /locus_tag="KLH32_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBE0139062.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18212..18922
                     /gene="ispD"
                     /locus_tag="KLH32_002258"
     CDS             18212..18922
                     /gene="ispD"
                     /locus_tag="KLH32_002258"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBE0139063.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18922..19401
                     /gene="ispF"
                     /locus_tag="KLH32_002259"
     CDS             18922..19401
                     /gene="ispF"
                     /locus_tag="KLH32_002259"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBE0139064.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19398..20447
                     /gene="truD"
                     /locus_tag="KLH32_002260"
     CDS             19398..20447
                     /gene="truD"
                     /locus_tag="KLH32_002260"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBE0139065.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20428..21189
                     /gene="surE"
                     /locus_tag="KLH32_002261"
     CDS             20428..21189
                     /gene="surE"
                     /locus_tag="KLH32_002261"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBE0139066.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21183..21809
                     /gene="pcm"
                     /locus_tag="KLH32_002262"
     CDS             21183..21809
                     /gene="pcm"
                     /locus_tag="KLH32_002262"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBE0139067.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21949..23088
                     /gene="nlpD"
                     /locus_tag="KLH32_002263"
     CDS             21949..23088
                     /gene="nlpD"
                     /locus_tag="KLH32_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBE0139068.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23151..24143
                     /gene="rpoS"
                     /locus_tag="KLH32_002264"
     CDS             23151..24143
                     /gene="rpoS"
                     /locus_tag="KLH32_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBE0139069.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24263..24670)
                     /locus_tag="KLH32_002265"
     CDS             complement(24263..24670)
                     /locus_tag="KLH32_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE0139070.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24817..25410
                     /locus_tag="KLH32_002266"
     CDS             24817..25410
                     /locus_tag="KLH32_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBE0139071.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25410..26357
                     /locus_tag="KLH32_002267"
                     /pseudo
     CDS             25410..26357
                     /locus_tag="KLH32_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26630..27193)
                     /locus_tag="KLH32_002268"
     CDS             complement(26630..27193)
                     /locus_tag="KLH32_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139072.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27358..27609
                     /locus_tag="KLH32_002269"
     CDS             27358..27609
                     /locus_tag="KLH32_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0139073.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27611..29707
                     /locus_tag="KLH32_002270"
     CDS             27611..29707
                     /locus_tag="KLH32_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0139074.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7301 a         7382 c         8059 g         7060 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggcgt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggct gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac cagccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcttggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt
    11761 attatgaatt tcaaatgaaa atcattaatt tattcataat gtaaatgttt tattggctga
    11821 tatatatcat agatagtttt taaaatatgc gtaaatatcc tttaagttta ctgaaggata
    11881 aaaatattgt gactttcttt gatttctggg gaaaaaaccg acgtggcgag aaagagggta
    11941 tcgacgacta tcaccttctt tgctggcatt tgctggatgt ggccgcaatg ggctatttaa
    12001 tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca
    12061 aggaacaggc agctcaattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg
    12121 cccgctcttt tcaacaactt tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa
    12181 aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa
    12241 gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga
    12301 tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg
    12361 aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca
    12421 agccactgtt tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac
    12481 ttataaaaca actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt
    12541 cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa
    12601 aggctttagt tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc
    12661 ctttcaccgg aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa
    12721 aggtgctgga tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga
    12781 ccggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta
    12841 acgctcaggg gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc
    12901 gactggtcaa aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga
    12961 taaaccggcg caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg
    13021 atgggtatca cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac
    13081 ccatcaagac gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg
    13141 gcagcgacgt tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct
    13201 tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc
    13261 atccttattt ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt
    13321 gtcgaatatt tctgcgtcat tacaacaccc agtgcctttc ggataatttt tattcaaacc
    13381 cggattggtc gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt
    13441 taatttcctt actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac
    13501 aattaagtta tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg
    13561 cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg
    13621 aataccatac tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa
    13681 acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc
    13741 agcatcgctc agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc
    13801 tttgccttca tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt
    13861 atcgcgggcg gtataaatat accgactatt aaatgtccga atatcactgc gataacccat
    13921 ttgctgaaac tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat
    13981 gcgtcccgga aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt
    14041 aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg
    14101 gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca
    14161 tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg
    14221 gtgccttaag cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact
    14281 cattctaatt ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg
    14341 caagaaaata gcggtattgc aaaggaacgg ttatggatca aaaacgactt actcacctgc
    14401 ggcaactgga ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc
    14461 cggtgatgct ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg
    14521 cgttttatcc aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc
    14581 gcgagatgta tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc
    14641 ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac
    14701 ataccgatat tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg
    14761 ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact
    14821 ctttccgtga ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca
    14881 actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga
    14941 ccgagcagga tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc
    15001 tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc
    15061 gtatcgacct gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg
    15121 gctgctggcc gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg
    15181 aagagatgct ggtttccacc accagtgaac gtcaggggcg cgtgattgac cgcgaccagg
    15241 cgggatccat ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca
    15301 caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc
    15361 ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt
    15421 ctgctgcacg atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt
    15481 aagcgtcacg gcacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa
    15541 gctgagcgcg aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag
    15601 cgtaaattta ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact
    15661 ggcgcatcga catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat
    15721 caaacccgtc gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg
    15781 atcaacaaaa tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat
    15841 tatctgacct ttgccgggca gctgccggat aatctggata tccgctttgt gccgctctcc
    15901 gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg
    15961 acactgctcg aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg
    16021 cgcttcccgg tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg
    16081 ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa
    16141 tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa
    16201 gcgatcaccc tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg
    16261 gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa
    16321 cagccgcttt ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct
    16381 cgtgttgatg gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa
    16441 aacctgccgc tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta
    16501 gatcgctatc agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat
    16561 gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct
    16621 gaattcagtg cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg
    16681 ggcgcgcgcg atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc
    16741 atagtcatcc ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc
    16801 tgtggtttac cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg
    16861 cgttacataa actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat
    16921 tatgcagcga tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg
    16981 aagtggcgaa tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac
    17041 accgcgccga acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt
    17101 ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag
    17161 cgcgtgccgg tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat
    17221 cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag
    17281 atctgttgag acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg
    17341 ttcgtcaaag taacaggatc aaatatgcgt aatagccata acattacact aacaaataac
    17401 gacagcctta ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt
    17461 atctcctggt tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc
    17521 ttctttctct acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg
    17581 attgcgctgc attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg
    17641 acagtaggca ttatgtttgg ctcactgttt atgtggctac tgggctaatt tgtactttct
    17701 tgtctcctct gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct
    17761 gggtatacgt aacaatggac aaatgtggta catttgcccg cgttgtcgcg gtatccccaa
    17821 cagaggatgt agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag
    17881 gatgggtaaa ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg
    17941 gttcggtaag aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca
    18001 agctacaaac gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct
    18061 caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc
    18121 gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca
    18181 aaacaatcga taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc
    18241 cgtggttccg gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct
    18301 ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg
    18361 ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct
    18421 ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct
    18481 ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc
    18541 ttgtttgcat caggatgacc tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg
    18601 agggatcctc gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc
    18661 cattgctcat accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc
    18721 tcgtgagctg ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga
    18781 cgaagcctcg gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga
    18841 taacattaaa gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac
    18901 catccatcag gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc
    18961 ggtgaaggcc caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg
    19021 cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg
    19081 ctgggggata tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc
    19141 cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac
    19201 gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc
    19261 gtgtttattg ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact
    19321 acggaaaaac ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta
    19381 ctcattaagg caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc
    19441 aaggcaccgg gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct
    19501 ttgagcctga tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata
    19561 cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct
    19621 tcgctgggca aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg
    19681 gcaaggaaat gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg
    19741 cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc
    19801 tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag
    19861 gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg
    19921 cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt
    19981 tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag
    20041 acgttaatca agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg
    20101 tagcaaccac cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga
    20161 taaccgccgc attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg
    20221 aacaagcagc tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg
    20281 aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg
    20341 acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca
    20401 gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatga tgacggggta
    20461 catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg
    20521 gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg
    20581 cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt
    20641 gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt
    20701 aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg
    20761 gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac
    20821 gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc
    20881 accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt
    20941 cgcgtgacgc gctgcggtac acgacatccg gcagatcagg tgatcccgca gcaagatccg
    21001 cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg
    21061 accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta
    21121 actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg
    21181 caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg
    21241 atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt
    21301 ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc
    21361 agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc
    21421 tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg
    21481 tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc
    21541 ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg
    21601 cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga
    21661 cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga
    21721 aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg
    21781 tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt
    21841 tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg
    21901 cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga
    21961 agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca
    22021 ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct
    22081 gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac gatgtctaca
    22141 gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg
    22201 caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc
    22261 gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc
    22321 gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac
    22381 cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag
    22441 gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca
    22501 gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa
    22561 ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac
    22621 aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc
    22681 gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact
    22741 gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc
    22801 gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt
    22861 aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt
    22921 gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa
    22981 aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt
    23041 tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc
    23101 aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga
    23161 atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg
    23221 tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg
    23281 aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg
    23341 agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac
    23401 tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa
    23461 aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca
    23521 acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa
    23581 catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta
    23641 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg
    23701 agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata
    23761 agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca
    23821 ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga
    23881 acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt
    23941 tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg
    24001 aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc
    24061 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata
    24121 tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc
    24181 tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc
    24241 gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca
    24301 gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag
    24361 gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct
    24421 tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt
    24481 ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag
    24541 gtttatcagc aatggcacgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt
    24601 gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc
    24661 gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac
    24721 gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata
    24781 ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca
    24841 ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat
    24901 gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct
    24961 tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg
    25021 gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg
    25081 aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac
    25141 gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc
    25201 atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg
    25261 cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc
    25321 gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg attaccgcag
    25381 gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac
    25441 aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg
    25501 atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt
    25561 ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc
    25621 agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg
    25681 agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct
    25741 gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc
    25801 gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc
    25861 cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg
    25921 gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata
    25981 aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca
    26041 tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg
    26101 cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt
    26161 ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc
    26221 cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg
    26281 ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga
    26341 ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa
    26401 agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt
    26461 ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac
    26521 aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc
    26581 aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg
    26641 ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc
    26701 taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta
    26761 gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc
    26821 tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta
    26881 gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt
    26941 caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct
    27001 tttttaactg atgtgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta
    27061 aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta
    27121 ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg
    27181 gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa
    27241 aataaaccat aaaaacacca aagaaccctt tacagggaat ctttggtatc ttaatcttta
    27301 tccatacgga tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg
    27361 agacaaagag aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa
    27421 gataacgggt ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca
    27481 ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata
    27541 ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag
    27601 gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc
    27661 agccaccaca aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt
    27721 tagtcgccgt cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat
    27781 aacacgaaag gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt
    27841 ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga
    27901 agcatatcgc ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca
    27961 gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc
    28021 cggtaacccc gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga
    28081 gctgccggag cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg
    28141 tactatcagc cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc
    28201 cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat
    28261 cagctggctg cctgaatttc tggcgcagta tcgccaggtg aacggcagac ccatgtcaga
    28321 agcctacgag gattttgtcg cagaatggca gcgtcgccat gcggacgaac cgtacatgct
    28381 ggaggtgatg ccttcctatg atgttgtccg gtatgccatg aaaaaactgc cggaagtggt
    28441 gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag cttgaaggat tcacccgccg
    28501 cgactggaca gctatgccgg tgaattatgt ctggattggt gacggtcacg gtatgaagct
    28561 gaaatgtgcg catccgatcc acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga
    28621 tggtggaaca cggtttgttg ttggctggag tcttgacctt gccgaaaacg tgttcgccgt
    28681 tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt attactcgga
    28741 taacggttcc ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta ttctgccacg
    28801 cctggggatt aaacacccta ccggtattgc cggtaatcca cagggacgcg gcattattga
    28861 acgcctgaac cggacactgc cgatgcgtat agccaggagg tatcgcacct attttggcaa
    28921 gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc
    28981 cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc
    29041 gtgggcggag cttattgagg ctattcgtga aggcgtggaa tggtacaaca accgcccgca
    29101 ttctgagctg ccgatgaaac cgaacggacg gcattacagc ccgacagagt tcagaaaaaa
    29161 acgccaggca gaagaagaca ccgaaattga gtggctgagt gatcttgagc tgcgcgacat
    29221 gttccgccca atggtggaac gaccggtaag acgctgcgaa attcagtggc tgaacaatat
    29281 ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat
    29341 tcatgacgcc gagcgaatta cggtacgtcg caaggacggc agctttatct gtgaggcgat
    29401 atggaacggc aataaacgcg cagcctttgc tgtcagtgct gaatatcaca aacaacagca
    29461 gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg
    29521 tattcagatt ctggaacaca aacaggctga accctggctg agtaatgttt atcgcccggt
    29581 tggtaatgtg gtggcagttc agcaaccgga atatgaagaa gagcatgacg aagaatttga
    29641 acgcgatttc cggctgggga tgcaaaaact ttttgccatg caggaagaag atgatccgct
    29701 ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa ataaaggaaa caaagctgat
    29761 ttgaaataac cgaggaaata ctatgactga tattgatgat gt
//
LOCUS       DAEEDS010000046        29236 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEEDS010000046 DAEEDS010000000
VERSION     DAEEDS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.046"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..218
                     /locus_tag="KLH32_002271"
     CDS             <1..218
                     /locus_tag="KLH32_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0139075.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KLH32_002272"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KLH32_002272"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE0139076.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KLH32_002273"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KLH32_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBE0139077.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="KLH32_002274"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="KLH32_002274"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBE0139078.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="KLH32_002275"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="KLH32_002275"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBE0139079.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="KLH32_002276"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="KLH32_002276"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBE0139080.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="KLH32_002277"
     CDS             7229..8179
                     /locus_tag="KLH32_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBE0139081.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="KLH32_002278"
     CDS             complement(8220..8690)
                     /locus_tag="KLH32_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBE0139082.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="KLH32_002279"
     CDS             complement(8745..9947)
                     /locus_tag="KLH32_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBE0139083.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="KLH32_002280"
     CDS             complement(10034..10576)
                     /locus_tag="KLH32_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139084.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="KLH32_002281"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="KLH32_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBE0139085.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="KLH32_002282"
     CDS             complement(11319..11921)
                     /locus_tag="KLH32_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE0139086.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="KLH32_002283"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="KLH32_002283"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBE0139087.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="KLH32_002284"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="KLH32_002284"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBE0139088.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="KLH32_002285"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="KLH32_002285"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBE0139089.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="KLH32_002286"
     CDS             16319..17209
                     /locus_tag="KLH32_002286"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBE0139090.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="KLH32_002287"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="KLH32_002287"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBE0139091.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLVLAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="KLH32_002288"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="KLH32_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBE0139092.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="KLH32_002289"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="KLH32_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBE0139093.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KLH32_002290"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KLH32_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBE0139094.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KLH32_002291"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KLH32_002291"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBE0139095.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KLH32_002292"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KLH32_002292"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBE0139096.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KLH32_002293"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KLH32_002293"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBE0139097.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KLH32_002294"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KLH32_002294"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBE0139098.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KLH32_002295"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KLH32_002295"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBE0139099.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KLH32_002296"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KLH32_002296"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBE0139100.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KLH32_002297"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KLH32_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBE0139101.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="KLH32_002298"
     CDS             complement(27384..27845)
                     /locus_tag="KLH32_002298"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0139102.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="KLH32_002299"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="KLH32_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBE0139103.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="KLH32_002300"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="KLH32_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7086 a         7856 c         7300 g         6994 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgtcctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAEEDS010000047        29200 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEEDS010000047 DAEEDS010000000
VERSION     DAEEDS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29200
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.047"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(177..1073)
                     /gene="yhaJ"
                     /locus_tag="KLH32_002301"
     CDS             complement(177..1073)
                     /gene="yhaJ"
                     /locus_tag="KLH32_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBE0139104.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTVEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1178..1879
                     /locus_tag="KLH32_002302"
     CDS             1178..1879
                     /locus_tag="KLH32_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBE0139105.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1902..2066
                     /locus_tag="KLH32_002303"
     CDS             1902..2066
                     /locus_tag="KLH32_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139106.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2278..3588)
                     /locus_tag="KLH32_002304"
     CDS             complement(2278..3588)
                     /locus_tag="KLH32_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBE0139107.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3616..4947)
                     /locus_tag="KLH32_002305"
     CDS             complement(3616..4947)
                     /locus_tag="KLH32_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBE0139108.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5222..6586)
                     /gene="tdcG"
                     /locus_tag="KLH32_002306"
     CDS             complement(5222..6586)
                     /gene="tdcG"
                     /locus_tag="KLH32_002306"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE0139109.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6658..7047)
                     /locus_tag="KLH32_002307"
     CDS             complement(6658..7047)
                     /locus_tag="KLH32_002307"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBE0139110.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7061..9355)
                     /gene="tdcE"
                     /locus_tag="KLH32_002308"
     CDS             complement(7061..9355)
                     /gene="tdcE"
                     /locus_tag="KLH32_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBE0139111.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9389..10597)
                     /gene="tdcD"
                     /locus_tag="KLH32_002309"
     CDS             complement(9389..10597)
                     /gene="tdcD"
                     /locus_tag="KLH32_002309"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBE0139112.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10623..11954)
                     /gene="tdcC"
                     /locus_tag="KLH32_002310"
     CDS             complement(10623..11954)
                     /gene="tdcC"
                     /locus_tag="KLH32_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBE0139113.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11976..12965)
                     /gene="tdcB"
                     /locus_tag="KLH32_002311"
     CDS             complement(11976..12965)
                     /gene="tdcB"
                     /locus_tag="KLH32_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBE0139114.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13064..14002)
                     /gene="tdcA"
                     /locus_tag="KLH32_002312"
     CDS             complement(13064..14002)
                     /gene="tdcA"
                     /locus_tag="KLH32_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBE0139115.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14192..14536
                     /gene="tdcR"
                     /locus_tag="KLH32_002313"
     CDS             14192..14536
                     /gene="tdcR"
                     /locus_tag="KLH32_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBE0139116.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14792..15331
                     /locus_tag="KLH32_002314"
     CDS             14792..15331
                     /locus_tag="KLH32_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139117.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15353..15700
                     /locus_tag="KLH32_002315"
     CDS             15353..15700
                     /locus_tag="KLH32_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139118.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15869..16519
                     /locus_tag="KLH32_002316"
     CDS             15869..16519
                     /locus_tag="KLH32_002316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139119.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(17140..17516)
                     /gene="rnpB"
                     /locus_tag="KLH32_002317"
     ncRNA           complement(17140..17516)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KLH32_002317"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17549..18694)
                     /gene="garK"
                     /locus_tag="KLH32_002318"
                     /pseudo
     CDS             complement(17549..18694)
                     /gene="garK"
                     /locus_tag="KLH32_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(18791..19681)
                     /gene="garR"
                     /locus_tag="KLH32_002319"
     CDS             complement(18791..19681)
                     /gene="garR"
                     /locus_tag="KLH32_002319"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBE0139120.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(19711..20481)
                     /gene="garL"
                     /locus_tag="KLH32_002320"
                     /pseudo
     CDS             complement(19711..20481)
                     /gene="garL"
                     /locus_tag="KLH32_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(20497..21831)
                     /gene="garP"
                     /locus_tag="KLH32_002321"
     CDS             complement(20497..21831)
                     /gene="garP"
                     /locus_tag="KLH32_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBE0139121.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            22206..23777
                     /gene="garD"
                     /locus_tag="KLH32_002322"
     CDS             22206..23777
                     /gene="garD"
                     /locus_tag="KLH32_002322"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBE0139122.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(23829..24653)
                     /gene="agaR"
                     /locus_tag="KLH32_002323"
     CDS             complement(23829..24653)
                     /gene="agaR"
                     /locus_tag="KLH32_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBE0139123.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            24902..26182
                     /gene="kbaZ"
                     /locus_tag="KLH32_002324"
     CDS             24902..26182
                     /gene="kbaZ"
                     /locus_tag="KLH32_002324"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBE0139124.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            26205..26678
                     /gene="agaV"
                     /locus_tag="KLH32_002325"
     CDS             26205..26678
                     /gene="agaV"
                     /locus_tag="KLH32_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBE0139125.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            26689..27468
                     /locus_tag="KLH32_002326"
     CDS             26689..27468
                     /locus_tag="KLH32_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBE0139126.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            27458..28267
                     /locus_tag="KLH32_002327"
     CDS             27458..28267
                     /locus_tag="KLH32_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBE0139127.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            28354..28788
                     /locus_tag="KLH32_002328"
     CDS             28354..28788
                     /locus_tag="KLH32_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE0139128.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            28785..>29200
                     /locus_tag="KLH32_002329"
     CDS             28785..>29200
                     /locus_tag="KLH32_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE0139129.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPP"
BASE COUNT         7593 a         7625 c         6892 g         7090 t
ORIGIN      
        1 attgttgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccagccta
       61 caaattgcca aaattcaata tattgcagtg actgcgtagg cctgataagc gtagcgcatc
      121 aggcagtttt gcgtttgtca gcagtcagaa gcaccttgtg aaaggtgctt aatagattat
      181 tttccagcga acaactttgg aatttcccgc agacaccagg atttcgcttc ccccatactg
      241 tcacgacgcc aggccataat aatatcgatc tcgctggtcg attccgggct gacgacacgc
      301 aaccgccctt ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc
      361 cccgccagta atgcctgacg tttatcttca accgtgctca ccgttaagcg cggctgtttg
      421 tccagcagct gtacggtcaa caccgggcgc tcacgagcgg tatccgctac cgcaattcca
      481 cgatatttca cacgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca
      541 ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc gcgagttgat ctccgacgag
      601 gaacgaaaat gcatatccgg cgcgataacg atatccgccc gcccctgctc cagccgttcc
      661 cacgcccccg ccagcacttc tgtgatgatt gccagttggg tattggcttt tgccgccagt
      721 ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga
      781 tgcgtttccc aaccgcgcgc gagagcttcc gcatcagtag tcagtttatc tgcggcttcc
      841 agcaaaacgc gccctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg
      901 cccgagcggt caaacagcac cacatccagc tcttcttcca gtttttgcat ggtgtagcta
      961 agtgcggaag gcacgcgtcc cagctcatcc gctgccgccg caaaactgcc ccggcgatcg
     1021 atcgcatcca taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc
     1081 tcattcagga aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc
     1141 ccttaccata aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag
     1201 gcagtgtgga caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca
     1261 ctacttcgac ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc aggaagtgct
     1321 ggccccaggt gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat
     1381 tctggatgga gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgccgg
     1441 agaggcgttg ctgctctcta cccagccggg tgtcagctat agcgaacaca atctcagcaa
     1501 agacaaaccg ttaacgcgaa tgcagctttg gctggatacc tgcccgcagc gagagaatcc
     1561 gctgattcaa aagctggcgc ttaatatgga caagcagcaa ttaatcgcct cgccagaggg
     1621 tgcgatggga agcctgcaat tacgccaaca agtgtggctg caccatatcg tgctcgacaa
     1681 aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg
     1741 gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg ctaacctgcg gtgatggggc
     1801 gtttattcgt gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct
     1861 gatagatttg cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcgacca
     1921 aaaagcgcca gccagcgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt
     1981 ttggctatga agagatgttg agcgagctgg aagctatcgt cgcggatgct gaaacgcgtt
     2041 tagccgagga tgaagctacc gcgtaaagac aggttgccgg atgcggcgta aacgccttat
     2101 ccggcctaca aaacacaaca acatcaacaa attaaacacc ccgtaggccg gataagatgc
     2161 gccagcatcg tatccggcat caggtacaaa acacaacaac atcaacaagt tgagcacccc
     2221 gtaggccgga taaggtgcgc cagcatcgca tccggcacaa tactcaaaac ctggcgttta
     2281 tctggccttg ctcgccataa tctcgataat ctgccgatcc gtttgctgca tcgaatggct
     2341 tgctaacgca cacaggttgg caatcgactg ctcaacatca tgcgccacga tcccttcatt
     2401 gccggtcacg gcggtatcat ccagcgccat taacaccgct ttccacgcag ccgaagcact
     2461 ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg caaatcatgc cgctgacatc
     2521 gccgatcata ctgctgatcg ccatcgagat agtttcataa cgcccatcca ccagccatgc
     2581 catcccggcg gcggccccca ttgctgcggt cgttgcggca caaagtgcag acaaacgcgg
     2641 taactggtta tggatgtaaa ttgcgctcaa atgagaaagc atcagcgcac gcgccagtcg
     2701 ttcatcatcc gctccgaagt gttctgctac aaccaccaca ggcattattg cagtgatccc
     2761 ctggttaccc gagccggagt tactcatagc cggaagcgta gcgccgccca tacgcgcatc
     2821 ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg
     2881 ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc
     2941 ctgcgataac gcacaattta gcttcgcgga atcgagaata aagcggatcg ccgcaaatgg
     3001 gacttcattg acgaacttca ggatctcagc cagcgtcgtt ctggaaagca ccgtcagcgg
     3061 cgactcttgc tcgccctctg ccacacacgc ctggtgggta aacaccacac cattgtgcgt
     3121 ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg acacacgccc acttctcacc
     3181 gttccagact ttggcgcgtg agaagaggat ttcattgcaa ggttcctgga tcttaacaga
     3241 gactttcccc gccgccagca gtgctttggc atcggcaatt gcctgcgcag ttgcgtcttt
     3301 cagcacttcc agcccggcgt tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg
     3361 cagccccacc attcccgtgc cgggaacggt gacgcccaga ccgttcttca tcagatttgg
     3421 cgaaacccag gcttctacac gttcaaccgg accttccagt tctgccgcag caaccgccgc
     3481 cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc ggttttactt cctcctgaac
     3541 ggcgaggatg taacgctgcc ataacggatt taaagtcgaa tcaaacatat taaaaacctt
     3601 aaaatttcag gtaaatcaag aaaatgccag gaacagagaa acacaaagca acaaaccagt
     3661 gacgataatc aggtacagag acatcccttt gtatttgtgc aatgccggta ctttgtaaac
     3721 cagccacgcc gggatcaggc accctaccat gccgaaaatc gggctacaga tagaggtgaa
     3781 gctcaacacc ggtgcgttca gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat
     3841 gatgccgcgc tgaacaagat tttcgttaat cttctcggca ggcatcttgc gacgcaggat
     3901 gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt
     3961 catgacagca aagatattga gaatgacgct gaccactttc acccacgctg cgccgtcacc
     4021 gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc
     4081 ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa
     4141 aatgccaaac gcgatattca ttgcccgcaa tgctttatgc cgcgccactt caattgattt
     4201 ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt
     4261 cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc agccccagcg gcggtagcga
     4321 accgacgtta tacagatgcc acattcccac catcgacacg cccagcgcag cgactaccag
     4381 cagcttggtc agcaccatgt cggtcgaaat tttgaataac aatttctcgc cgcgtgaaga
     4441 gatcgccacc agaatgcaaa tcagtaccag accataaaag ggactgtctg acagcaaccc
     4501 ttccgtcacg ccgaaggtat gcagatagga agcactatcg ttggtgatgg cggtggaata
     4561 gacgaacatc caaatcacca gcattacgaa atagagcgca cctaacagga tgccccagtt
     4621 tttacctaaa taaccgctaa tgacactcgg gtaatcttta cattctggtg attctgccag
     4681 cgtattaata aacaaccgct gaaacagata cattgccggg taaccaatca ccgatgagag
     4741 caaaaatacc cacaatccca tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc
     4801 aatcgccatc ccgatactca taatcaccca gccggtgtca gtactgtcga atttgatcgc
     4861 ttctcgccac tcgctctcac tcattccagc acgacccgcc ggggtcgaag cgtctgcaat
     4921 gacgccttta ttcgatgcaa tttccataat ttctcgctca atattttgta gggcttatta
     4981 tttttttccg agccgcatca aggcgatatg cggtctacgt ttttgcaggc gagttattag
     5041 aataaagaaa tgatacgcgc gaggtcagag aaaatcttca caatcaagac tttaaaataa
     5101 cggagatgga taagaatttt ctactaaatt aatcgcaaga gaatattcat tctctatttg
     5161 cggcagatca caaaaaaaag gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat
     5221 gtcagccgca gaccacttta atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt
     5281 tcatatcttt gccggtttca tacatcgtct cgatcacttt atcgagtgaa acacgcggtg
     5341 ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc tttcacggca ttaatggcat
     5401 tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg atcgcaggtc agcccaaggt
     5461 tatgctccat cgcgatttcc gccgcattgc atacctgcgc cggactgccg cccagtagtt
     5521 cagttaaccc tgccgctgcc attgaacagg ccacgccaat ctccccctga cagccgacct
     5581 ccgcgccaga gatggaggcg ttcattttat acagcgcgcc aatagccccc gctgccagaa
     5641 aatagcgggc aattgaccgc tcgtttaccg gacgacggaa cttatcgtaa taagccagca
     5701 ctgccggaat aatgccgcac gcaccgttag tcggtgccgt taccacgcgc ccgccagctg
     5761 cgttttcttc actaaccgcc agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat
     5821 cgttagagat gttatcgcta gaaaccagct gacgacgcag cgctacggca cgacacggca
     5881 cattgagcgg accaggcagc acgccttcag tgttcatccc gcgttcaata ccgtcatgca
     5941 tcacttgcca gatacgggca aaccctgcgt caatttccgc tttgctgcgc agcgccagct
     6001 cgttgtgcat catcaggcca gatatagaca ggccgttgta atcacacatt ttcagcagtt
     6061 cacctgctga gtggaaatcg taaggtacgg acgtttcgac atcgtgcgac aggccgaagt
     6121 gttcttcttc gacaataaac ccgccgccga cagagtaata ggttttactt aatagcgctt
     6181 cctgtccctt ccaggcagtg atccgcattc cgttctcatg gcgaggcaac atttcgggat
     6241 ggaagataat gttctttgct acaggaaaat caacaatatg cgcaccagat gccactggca
     6301 gccgcccgct gcgcgttact aactctataa atgcagggat ctcatcaatg acaacatcct
     6361 gcggactgtt tcctgccagt cccatgataa tggcgacatc cgtggcatgg cctttgcccg
     6421 tcagtgacaa cgacccgtac agatcgacca caatatggct cgtcgcggtt aataagccgc
     6481 tactttccag ccgatcaata aaactttttc cggcattcat tggccccacg gtatgcgaac
     6541 tggagggacc aatcccaatt ttgaaaatat cgaatgcact aatcatatcc acaccctcgg
     6601 attgccgttc agtgaagtgg agcggaacga ccttgcgacc gtcccgctca cgaggcttta
     6661 cgcactacgt actgcgatag cttcaatttc cagcttcaca tctttcggca aacgcgcaac
     6721 ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca tcgaagaact gcttatagac
     6781 ttcattgatg gtggcaaaat cattcagatc ggtgatgaac acggtcatct tgatgatatc
     6841 gcccacgctc agcccggcag caaccacgat cgctttgacg ttttcgaggc ttaaacgtgc
     6901 ctgatcttgc acatcagccg ggatctcacc ggtctgcggg caaaccggaa tttgcccgga
     6961 ggtgaagacc atgctgccta aatcaacgcc ctgaacataa gggccgattg cgcctggggc
     7021 acgttgcgtt tcgataatct ttttcataca tcctccggcg tcagagcgcc tgggtaaaag
     7081 tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc
     7141 cagagacacg gattgtcagg ttaggatatt tttccgggtg ctcgatggca tccagcagca
     7201 tttcccgatt cattacgttg acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt
     7261 ggaaataccc atccagcagg ccaacaaggt tggttttacg tactggatct tctttgccca
     7321 gcgccgcagg aacgattgag aaggtgtacg agatcccatc tttggcgtag gtgaacggca
     7381 gtttcgccac cgacgtcaat gaggccacgg cacctttgcg gtcacgacca tgcatcgggt
     7441 tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc cggcgtatta ccggttttct
     7501 ggccgtacac cacgttagaa gtgatagtca gaatcgactg ggtagggacg gcgttgcgat
     7561 aggttggcag cgctttaatt ttcttcataa agcgttcaac caggtcgcag gcaatgctgt
     7621 ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc gtcgatttca aagtccaccg
     7681 ccaggccgtt ttcgtcacgg attggtttca cgcgggcata tttgatggca gacagggagt
     7741 ccgtcgccac cgacaggccc gcgatgccgc atgccatagt gcgatagaca tcacgatcgt
     7801 gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg catgtagtga atgatattca
     7861 gcgcgctgat gtactgcacc gccagccagt ccatgaagtg atcgaggctg tccatcactt
     7921 tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt cggcccgacc tgaatcttca
     7981 gcttctcgtc caccccgccg ttaattgcgt agagcagcgt tttcgccagg ttagcgcgtg
     8041 caccaaagaa ctgcatttgc ttaccaatca ccattgggct gacgcagcag gcaatcgcgt
     8101 aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt ttcatactgc aaggaagagg
     8161 tgacgatcga cacctgcgcg gcatattttt tgaaggcaat cggtaattct tccgaccaaa
     8221 gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga
     8281 aggagttttt ggtcaccagc gtacgaccgt ccagccccat cccgccgatc acttccgtcg
     8341 cccagattgg gtcgccggag aacagcgaat caaattccgg tgtacgcagg aagcgtacca
     8401 tacggatctt catgatgaag tgatcgatca gttcctgtgc ctgctgctca ttgaggacgc
     8461 cagctttaaa gtcgcgctca atgtagatat ccaggaacga tgccgtgcgc cccagcgaca
     8521 tcgcgccgcc attttgcgat ttcactgccg ccagataagc gaagtagagc cactgcaccg
     8581 cttcctgtgc attctgcgcc gggcgagaga tatcaaagcc atatttcgcc gccatttcct
     8641 gaatctgcaa cagcgcatga cgatgctctg ccagctcctc acgcagacgg atggtggctt
     8701 ccagatcctc gcctttttcc agacgagact ggagatcggc aaattgcagt tcgcgttcac
     8761 gcaccagata actgatgcca tacagcgcta cgcggcgata gtcaccgata atgcgcccac
     8821 gaccatagcc atctggtaaa ccggtcagca cgccagattt acggcagcgc agcatatccg
     8881 gtgagtaaac atcaaatacg ccctggttat gggttttacg cagatcggta aacagatatt
     8941 caaattcact gtccatttca cggccatagg cgtggaatga acttttaatc atattaatgc
     9001 caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt
     9061 ccagcggctg gttaatatat cccgcatcat gagcggtaat tgtggtggca atattggtat
     9121 cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat gccttccatt accttttccc
     9181 acaattccgt ggtggcaggc gtcgcttcgg cgagaaaaga ttcatcgcct tcatacggtg
     9241 tatagttatg ttgaataaaa tcgcggacat taatttcgct tttccagtcc gtacctttaa
     9301 agccaagcca tgcgtcagcg tacagcttat cgctggtatc aatatctacc ttcatgaaaa
     9361 ataatctctc tacaatactt caactaaatt atgcaaattc tgcgggcgcg ttaactttac
     9421 ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt agtcggaata acgacgcaaa
     9481 tgacatgcgc attttcactg gaaacaattc gctcgccaaa agagttagat cgattattca
     9541 tttctgtatc aatctctacg cctaatacag ccaaatgttc catgaccaga cgacgaatta
     9601 aacttgaatt ctctcctatt ccgccggtga atataattcc atccagccga cgtaatgaag
     9661 ctgcatgtcc ggcaatatga cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg
     9721 cgcgttcgtg accttcatgc caggcttttt ccagaacacg taaatccgaa gaaagaccgg
     9781 aaatacctaa taatcccgac tctttattca ctacgcgttc caggtcaccc aggctctggt
     9841 tggtttggct ggcgacccag gacatcgcac caaagtcgac atcgccactg cgggtaccca
     9901 tcatcaagcc ttccagcggc gtcattccca ttgaggtgtc aacactctga ccgttgcgaa
     9961 ctgcgcagat tgacgcgcca ttgccaagat gcgccacaac caggccggag tcatcttccg
    10021 ccagattcag cagcgaatgt gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac
    10081 cataacggcg tacacctaac tcttcataat atttccacgg caggccgtat aaataagctt
    10141 ccggagccat cgtctggtgg aaactggtat caaataccgc cacctgagtt acgcccggaa
    10201 ataattgctg cgccgattca ataccactta aatttgcgta attatgcagg ggtgccagtg
    10261 gagaaacgcg acggatatta tcaatgactt catcggtaat aatggcggac tcggtaaaaa
    10321 tactgccgcc gtgagcgatg cggtggccaa ttaaggccac gctgtcattt aaattccgtt
    10381 tttccagttc aaatgcaatt gccttcaatg caccttcgta gctgtggtga gccagcggtg
    10441 ctggctctcc cccatttacg gataagaatg cattttccga gttaataccg tcggcaatac
    10501 ctgacattaa tacttcacaa tcgctggcat cgagtacgga aaacttaatc gaagacgaac
    10561 cacagttaat aaccaaaaca accggaaatt cattcatctc ttttctcatc ctgagttacg
    10621 gattaaaaca gtttgtatac gatgttcagg atggtcagca gaccaatcac ggtaacaaac
    10681 acgttatcca gacgaccacg gtatttcgcc agagacggcg ctttacggat agcatacatc
    10741 ggcaacaggc acagcaggga tgcgataatc ggtgcgccca tggcttcaat caggtcgagg
    10801 atgttcgggt tggcgtaggc aacaacccag gtggagccca tgatgaagat catgctgaga
    10861 gtattcagtt tacccagcga cactttggtt ttgtcacctt tataaccgaa cttcagaatc
    10921 agaccattca agccttccag cgtccccaga tagtgaccga agaaagattt gaagatagcc
    10981 acgagtgcga tgatggaagc cgcatattcc agtgtaatcg cgaacgttgt tttcgtaccg
    11041 gtcatggacg caaagtggtt agccagataa gaaagcactg gaatattctg cgctttggct
    11101 tccgccatgt tggccggaga cagagtaaac aggcagctaa aggcaaagaa catcaccact
    11161 gcaaccatca gcatgctggc acgagaaatg atttgggaac atttacgttc ggtgaagtcg
    11221 cgaccgaagt ctttctcata ctcttcacgt ttagaaacca cgaaggaaga gacgattggc
    11281 gagaagttaa aggagaaaac catgatggaa atccccagcc agacagtgat caggataccg
    11341 tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt cgataactgc agagttccag
    11401 taagggatca gcgacagaga aatcagcacc aggctggcga taaacggcca taccaggtag
    11461 ctcatcactt taaccatcag atccttacca aaccagatga cgaaagccat cagcagcaac
    11521 aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac
    11581 gtcataaagg tattggtaat agtaacgcca taaatccaca gcagtgggca aatcgcgaag
    11641 aagtacagga acgtgataac cacgccgcca gttttaccaa aatgctcttc caccgtttcc
    11701 gtaatgttgc cggaagggtt agagccagaa agacacagac gcgccagcgc ccggtggcaa
    11761 taaaatgcga tggggtatgc caataccaac atcagaagaa tcgggatcag tccgccaaaa
    11821 cctgcgcgga tagggaagaa cagcaccccg gcgccgattg ccgtaccaaa caagcctaac
    11881 gtccatgtgg tatctgattt acgccaggac gattgttttg tctggctgga tacaatgcta
    11941 tctgaagtac tcatatccta tcctcaacga attaattaag cgtcaacgaa accggtgatt
    12001 tgagagacgc gagaaagatc gatattgccg ccggaaataa tactgacggt ttttctgttt
    12061 tgaatatatt ggtctaattt accgcttaat aatgcagcac atgccagagc gcctgcgcct
    12121 tcggtgacga ctttattgcg ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg
    12181 ctgaccagca cgatgtcatc gactaattca cgaacgattt cgtaagttaa attacccggg
    12241 cgggagacat cacaaccatc cgccagggtg ccggtagttc ggtgcgtggt tatttctccg
    12301 gagtggaaag aagccgccat gccgtgaacg ttttctgact gtacgccaat aacacgaatg
    12361 gtcgggttaa tagatttaat tgccaccgca ataccagcaa ttaaaccacc accaccaatt
    12421 ggcacaatca cgttatcgac atcatagaga tcttccataa tttccagacc aatcgttccc
    12481 tggccagcaa tcactttcgg atcatcgtaa ggtgggataa aaatacgacc ttccatttcg
    12541 acaatttcgc tcactttagc gatagtgtcg ttgaagttat caccatgcag aacgacttct
    12601 gcggagtagt cgcacgttgc cgctactttg gattttggcg caccttttgg catcaccact
    12661 ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca
    12721 gaacaggcca ccacgccttt gcgtttttcc gcatcggtca gtgaacttaa tttattaaat
    12781 gcgccacgaa ttttaaatga acccgtacgc tgcatatttt caaacttcag gaatatttca
    12841 cctttgcaac gttcactaaa atagttggag cgaggcatgc ctgttttata aattcgccca
    12901 gccagtcgtt gtttcgcttc aataatatca tcaatagcaa ccggcagatc gtatgtaata
    12961 tgcattataa aacctcttcg cctgaattaa atatgtaggt aaccgtaacc gacacctgca
    13021 aaacagacag gtggattatt tatgtttaaa taaaaacaaa caactaacca acttctatta
    13081 attgccttcg tctacaccca ttataagatg aatactcttt ggctaattcc accaaaaccg
    13141 atgctgcttt tttaatacga taatttttcg accataccgc ggcatattgt gccacaggta
    13201 atgtttcttc aaccggaata gtaataaatt gattagaacc aaaaggtgac gtcatatcac
    13261 aaggaattac agttaagaaa tcagcattga gaacaagatt ataaattgtc acgactgagt
    13321 cggttttaac gatgttttca atactgatgc catttctttg taacgtagta agcagttcgc
    13381 tgtagtaccc catattagtt tgtggcaaca cccactgttc gttcttcaac gactccagcg
    13441 tggtggtgcc ggtgcatgtt cgggacttac tggctaccag cacaaactcg gactcgaaca
    13501 gcggctcaac atgtaaatcc tgaagcttca tttctgcact taacgtacca atcgcaaaat
    13561 ccagtcgacc gtcgcggatt gccggtaaga acgaagacag ttgcgcttca tacatagaaa
    13621 cctgcgcttt cgggaacacc tctttgaact tgttgatcat ccctgacata aaagtaaaac
    13681 caatcaatga aggaaaacca aatgagactt ccaccaccgc ttcagaagac ataccgctta
    13741 tctcattaac catatttttc atttcacggg taatggattc ggaacgggag agtaacaatt
    13801 gaccggcagg tgttaatgtt acaccggtat ttttccgcac cactaattcc acaccaaaat
    13861 aatcttcaat atcgttaatg attttactga cggccggttg agttaaccct aattcttttg
    13921 cagccgagcc gatagagcca cttctaatga cttcctgaaa gactaccagg tgctgcgttt
    13981 tcggaagaag aatagtgctc ataacgacct acgttaatta cctcattgac ggcatgaagt
    14041 gtatcaaaat gaaataaaca ggatatgtgc gaccactcac aaattaactt tcattacttt
    14101 ccaaggtatc gttattacaa ttaaatatct ttaaatatca acaagttaaa gcataaaaat
    14161 cagcataaac cctgattttt atcaaaaaaa aatgacgggg ataaccatat tttatggcga
    14221 taacatcatt cgttatgtgg ttaatacaaa aaaggggctg agaccatatt tcaagcaatt
    14281 accggataat taccaggcaa aatttgagtt aaatttaatg agcaagttct caaattttat
    14341 tataaataaa ccattttcag cgataaatac tgcggcacgt cacatttttt cacgctattt
    14401 gttggagaac aaacatttat tttatcaata ttttaaaatt tcgaatacag gtattgatca
    14461 tctcgaacaa ttgattaacg tcaacttttt ctcttctgac aggacgtcat tttgtgaatg
    14521 caatcgtttt ccataaattc ttctcccctc ataggcgacg aatagcattt tgtgttgagg
    14581 atcacaaaac gaataattgc tggtcgccgc gataaggtca gacgaagtca acaaggggga
    14641 ttttcactgt gctttacatc agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc
    14701 gggtaaatga gcgttttttg atagtgcgta agtgtcgtgc gaggaaaata agcatctatt
    14761 attgtttgtc atgacgacaa ggcaggcatc aatgaaaggc ttcccaattg cacatatttt
    14821 tcacccttca atcccgccaa tgcacgcagt ggttaacaat cacaatagaa atattgatta
    14881 ttggacggta aaaagaaagt ttgcagaaat tgtctccacc aatgacgtta ataaaattta
    14941 cagtataagt aatgaactcc ggagagtatt atctgcaatt actgcattga atttctatca
    15001 aggcgatgtt ccttctgtca tgatccgaat ccaaccggaa aatatgagtc cattcatcat
    15061 agatatttct acaggagaac atgatgatta tatcatacaa acattagatg taggcacttt
    15121 tgcacctttt ggtgaacaat gtacttgctc agccgtcaat aaaaaagagc tggaatgtat
    15181 taaggagacg atttctaagt attgcgcaaa gttcacccaa aaagaagcca tcttaacacc
    15241 ccctaggcac tttaataaaa cctgcattac ttcagattgt tggcaaattc tctttttttc
    15301 acccgatcat tttaataatg atttttattg attatttata aaggataaaa taatgttccc
    15361 tgtttcatca attggtaatg atattagtag tgatttagtc cgtcgaaaaa tgaatgacct
    15421 tcctgaaagc cccataggaa ataacctcga agcactagca ccaggtatag aaaaactaaa
    15481 acagacctct attcaaatgg tcactttact taacgcgtta caaccaagtg gaaaatgcat
    15541 tatcactggt gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatga
    15601 ctcgtctctt cgtctggatt ttttaggtta taacacccaa attattcaac gatcggacaa
    15661 tacctgcgaa cttaccatta atgaaccgtt aaaaaactag gaaatatcta caggcaatat
    15721 caatgttaat tgcccattaa aagatattta caatgaaatc aggaggttga acgtaatctt
    15781 tagttgtggg actggagata tagttgatct atcctctctg gacttacgta atgtcgattt
    15841 agattattat gatttcacag ataaacatat ggctaatact attctaaatc attttaaatt
    15901 ggattcaaca aattttacta atgccaacat gtttcaggtt aattttgtta gttcaacaca
    15961 aaacgccaca atctcctggg attatttact aaaaataacg cctgttttaa taagcattag
    16021 cgatatgtat tctgaagaaa aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga
    16081 cattaccgaa gaacaattaa aaattatgag atttgcaatt ataaaatcta taccaagggc
    16141 aactcttaca gataaattag aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa
    16201 aatcaacaat tgcttgaaca gaattaaatt accagagatg aaagaattct catcaaaaaa
    16261 aatatatgat tacatcgata caatcattga agattatgaa aatatcaaag aaaatgctta
    16321 tctggtcgtc ccccaaatta attatactat ggatttaaac atagaagact ctagctcaga
    16381 agagttaata ccctcgagaa agacgaaaat tctccggaca atggctttga ggtcggggaa
    16441 tataacacat atgaagcata taactcagag aatcaatatt ttaccagaga ggactatacg
    16501 tataattacg accttttaaa tgcaatatag acttaataac ttcttacaaa gtctattatt
    16561 aataccgtag atattcatct taccctgtgc accaaaagca aaaacttatc gacgcgggtt
    16621 tgaaactgat ggcaaccgca aaaatgcctg atgcgctacg cttatcaggc ctacgccatc
    16681 tctgcaatat attgaatttg cgtgcttttg taggcaggat aaggcgttca cgcagcatcc
    16741 ggcatgaata aagcgcactc aacaatctga aacccgccgg aacggtttat tacgtacatc
    16801 aggtgaaact gaccgataag ccgctttctt ttgggtatag tgtcgtggac agtcattcat
    16861 ctttctgccc ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtat atcaggtgaa
    16921 actgaccgat aagccgcttt cttttgggta tagcgtcgtg gacagtcatt catctttctg
    16981 cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg tacatcaggt gaaactgacc
    17041 gataagccgc tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc
    17101 aaaagcaaaa acccgccgaa gcgggttttt acgtaaaaca ggtgaaactg accgataagc
    17161 cgggttctgt cgtggacagt cattcatcta ggccagcaat cgctcactgg ctcaagcagc
    17221 ctacccgggt tcagtacggg ccgtacctta tgaaccccta tttggccttg ctccgggtgg
    17281 agtttaccgt gccacggact gttaccagcc gcgcggtgcg ctcttaccgc accctttcac
    17341 ccttacctga tcccgcttgc acgggccatc ggcggtttgc tctctgttgc actggtcgtg
    17401 ggtttccccc ccaggcgtta cctggcaccc tgccctatgg agcccggact ttcctcccct
    17461 ccgcccgtct cccccgaaga ggacgacgac gaagcggcga ctgtctggtc agcttcggcg
    17521 cgcagtatag agggtttgcg cgcccttgtc accccgtgtt gcgcattcca atcgccagtg
    17581 tcgcggcaat attacgtgaa gcacggcaga tattgtcata agccccgcgg aatgcttcgt
    17641 ccaacgtacc tatgctggtc aatacgctga agaccgcatc aatgccatgc tgatgtacaa
    17701 cgccaacatc atcggtcagg ctacccgcaa tgccaatcac cggtttatgg tacttcttcg
    17761 ccacgtttgc gacgccaatc ggtaccttgc cgtgaatact ctggctgtca atacgccctt
    17821 caccggtgat caccagcgta caatcgtgaa tatgttcctc cagattcagc gccgtagtga
    17881 cgatttcaat accacttttc agttccgcac caagaaacgc catcagcacc gcacccatac
    17941 cgcccgcagc tcctgcgccg gggacatctt ttacatcaac atgcagcgct tttttaatga
    18001 catcggcata gtgagagagg ttattgtcca gctcaacaat catcgcttca ctggctccct
    18061 tttgtgggcc aaagatgcgc gatgcgccgt tatcgcccac cagcggattg gtgacatcac
    18121 aagcgacgcg aatgacgcaa tcttttaagc gcggatcgag gccggaaata tcaatatcat
    18181 tcagagtatt aagactaccg ccgccaaaac caatttcatt gccgttggcg tcgcataatt
    18241 tcgcccccag cgcctgtacc atgcctgcgc cgccatcatt tgtagcgcta ccgccaatgc
    18301 cgataataat gtttgtcgcg ccgctctcca gcgcctacag gattaactcg cctgtgccgc
    18361 gtgaagtggt cacgagtgga tcgcgttttt ccgcaggtac cagctccagc ccactggccg
    18421 ccgccatttc aataaacgcg gttttgccat cgccggagat cccccaactg gcattcactt
    18481 tctcgcccag cggccctgta acccaggcgt gacgttcaga accctgggtg gctgcaatca
    18541 tcgcttccac cgttccttcg ccaccgtcgg caaccggaat agaaacgtac tgtgcatcag
    18601 gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca gataaacttt
    18661 ctttataaga gtctggggcg attacgattt tcatacctat gcctgttacc acatgacgcc
    18721 ggagggcgtt tctcttattc ggcctggatt ccaggcccgg attgcaatac gccatccggg
    18781 cacgacgtca ttaacgagta acttcgactt tcgccagttt ttcgtagtag cacgccaggg
    18841 cgctatgatc cgccgttcct aaaccatctg ctcgcagtgc ctgcatcatc tccataaccg
    18901 cagctgtgag cggcagttgc gcgccgacgc cgtgggaagt atccagcgca tttgccagat
    18961 ccttaatatg cagatcaata cggaagcccg gcttgaagtt gcggtccatc accatcggcg
    19021 ctttggcatc cagcacggta ctgcccgcca gtccaccgcg aattgcctga tacaccaggt
    19081 ccgggttaac gcccgcttta gttgccagcg ttaacgcttc tgacatcgcg gcaatattca
    19141 gcgccacaat gacctgattc gccagtttgg tgacgttacc tgcaccaatt gccccggtat
    19201 gcaccacgga acccgccatt gctttcatca aatcatagta tttgtcgaaa atagccttgt
    19261 cgccgcccac catcactgac agcgtaccgt cgatggcttt cggttcaccg ccgctcaccg
    19321 gagcatccag catatcaatg cctttcgctt tcagcgcttc gctgatttca cggcttgcca
    19381 gcggtgcgat agaactcata tcgatcaata ccgtacctgg cttcgcgcct tcaataatgc
    19441 cattctcacc cagcgccacc tctttcacat gaggggagtt tggcagcatg gttatgatga
    19501 cgtcgcactg ttcagcgatc gctttagccg tagaggctgt ttctgcacct gcagcaatca
    19561 cgtcagcaat agcttctggg ttacggtcag caaccaccag cgagtaacct gctttcagaa
    19621 ggtttttact cattggttta cccataatcc ccaggccaat aaaaccaact ttcatagtca
    19681 tatcaatcat ctctcttgtt gcggtggtgg ttatttttta aaggtatcag ccagtttatg
    19741 agtggcagag cggaagacgc cgagatcgct gccgacagcc acaaacgtcg cgccccattc
    19801 cagataacga cgcgcatcgg cttcgaccgg cgcgaggata ccgctgggtt tgccgtgcgc
    19861 gctggcacgg ttaaaaatgt gctgaattgc tttttgtaca tccgggtgtg atgcattgcc
    19921 gagatggcct aatgccgcgg ccagatcgct ggggccgacg aagatgccgt ctacgccttc
    19981 ggtagcggca atggcatcga cgttatctac gccctgctga ctttctatct ggaccagaat
    20041 agtgatgttc ttgttcgact gagcgaaata atccgccacg gtgccaaaca tattggcgcg
    20101 gtgagaaacg gagacgccgc gaatgccttc cggtgggtaa cgggttgatg ccaccgcctg
    20161 ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag ttatagaaac cgatatccag
    20221 aagacgctta ataatttccg gctcgttggt cggcactcac actactggcg cgctggcgct
    20281 gcctttcaag gccattaact gcggaataaa cgtggagata tcgtttggcg catgttcgcc
    20341 atccagcacc agccagtcaa acccagccaa accaagaact tcagtgctaa tcgggttaga
    20401 gagtgctgac cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt tgaatttatt
    20461 cgggaaaaca tcgttattca tcgcttatac ctttgcttat ttctgcaatt ccatacgttt
    20521 aatgtcgcca actacgaaga ggtagcagac catcgccatc agcgctgaac atcccacgaa
    20581 aaccagtgct gcattgaagg agtgcagttc acttaccagg tagccaatca ccagtggagt
    20641 gacaatggag gcaacattgc caaagacgtt aaagacgccg ccacagaggc caacaatctc
    20701 tttcggcgcg gtgtcagaaa tcaccggcca gcccagagca ccaaatcctt tgccaaaaaa
    20761 agccagcgcc atcagcatga ccaccagcgt ggtgttgttg gtgtagttac ataagatgat
    20821 ggtggaagcc agcaacattc ccagcacaat cggtagctta cgtgccaggg tcagggataa
    20881 accgcgtttg atcagataat ccgagaagac acctcccagc acgccgcccg caaaaccaca
    20941 cagtgccgga atcgaggcga ccagacccac tttcagaatc gacatgcctt tttcctgcac
    21001 cagataaatc gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa aatattgtcc
    21061 gaaaaatacg cccaacatca tgcggttaga gagcaattgc ttgatgtaat gcagtttgag
    21121 accgcttgct gccgcgctgc ccggcttttt gtggtccata tcgaccaccg cgccattttc
    21181 agagataaac ttcagctctt cctcagacat acgtgggtga tctgtcgggt tatgaatcaa
    21241 cttgatccac agcgccgtca gcacaaaaca aatcaccccc ataacggtaa agacgtgctc
    21301 ccatccccag gcgaaagtca gccagccaag cagaggcgaa aagagcgcca gcgagaaata
    21361 ttgtgccgag ttaaagatgg cggaggcagt accacgttct ttcgtcggga accaggcggc
    21421 gacaatacgg gcgttcgccg ggaatgatgg agcttccgag aatcccagca taaagcgcat
    21481 aaagaacatg gagatccctg cccaggccag cgggaacata tcaacaaagc cttgcaggaa
    21541 ggtgaacagc gaccagaaaa agaggctgta ggtgtaaact tttttcgagc caaacttatc
    21601 aagcaaccag ccgccgggga tttgcatcag caagtaggcc cagccaaaag cggagaagat
    21661 gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac cagcaataga
    21721 cagcgttgca cgatcggcgt agttaacggc ggtaacaata aaaataatca gtaatattaa
    21781 atagcgggta tgcacacctt tccttttttc gtcaacggtg tccagaatca ttttatttac
    21841 ctcgggtact tatgctgatt tttattatta tggggaaggt gttatttata agtttcattt
    21901 atgccgtaac gacaatgaac tcgggaatta gtataagcag cgcgagaata ataatcattg
    21961 tgcaaatgct aatttaatta atacttttta aatattattt tgagcatatg cacataaggt
    22021 tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcactgcaa tccctgaaat
    22081 gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa
    22141 atcatttatt tttaaatgag accaggtcct cattttaata acccctggct ggagaatatt
    22201 gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc
    22261 acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt
    22321 ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg
    22381 acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgtg
    22441 caatcccacg cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt
    22501 tacacacgct gccactggca accaaagtcc cggaaccctt accgccgctg gaaggataca
    22561 cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca
    22621 ccaccagcgt ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg
    22681 atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt
    22741 gtggcgtggc gattaacgca ccggcagcag ttgtacctat tcgtaccatt cacaatattt
    22801 cgctgaaccc taactttggc ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc
    22861 agcctgagcg cctgctgact ggaacggatg atgtgcaagc tattccagta gaaatcgcca
    22921 gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt
    22981 tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg
    23041 cttcagaact ggttgtcggc atgcattgcg gtggcagcga tgcgttttct ggcgtaacgg
    23101 caaacccggc agttggctat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt
    23161 tttcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg
    23221 aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg
    23281 gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg
    23341 tggtagagaa agcgctcggc tccattgcta aatcgggcaa aagtgcaatt gttgaagtgc
    23401 tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg
    23461 attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg
    23521 gtcgtggtac gccgtacggc ctgatggcgg tacccgtcat taaaatggca acccgcaccg
    23581 agctggcgaa ccgctggttt gatttaatgg atattaatgc gggcaccatc gctaccggcg
    23641 aagaaactat tgaagaggtg ggctggaagt tgttccactt tattctcgac gtcgccagcg
    23701 ggaagaagaa aaccttctcg gatcaatggg gactgcataa ccagctggcg gtgtttaacc
    23761 cagcaccggt gacctgataa ttcaattcgt attatgcccg atagcacacg tttatcgggc
    23821 atattgagtc acagcgaaga cgcgtgatcc ccgaccagaa tcacttcaac cccagccttt
    23881 cgcagtcctt ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt
    23941 tgagtatcaa tgatcttatg taaactggaa cgattgaatt tactggaatc ggtgaccacg
    24001 atgatccgtt ccgcaacttc gcacatccga cggtttaaac gggcttcatc ttcattatgt
    24061 gtgctgacgc cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg
    24121 taattttgca gcgattgctc agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa
    24181 tgcccgccgg tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc
    24241 ataccgttgg tcatcgcaat tacgtcagtg tacttgcgca tcagacgagc aatctcaaaa
    24301 gtggtggtcc cggaatcgag gatcacccga tgacctggct gaatcaactc aacggcagct
    24361 ttcgcaacgc tgcgtttcat cgcggtgttc agtgcgcttt tatcttccac tgatggctcg
    24421 actgacggcg tcgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc
    24481 tgcttttcca gaaacgccag atcattgcgg atcgtcacag tagatacgcc atacaatgcc
    24541 gacagatcgt taacctgcac actcccttgc tgtcgcagac gctgaatgat ctgttctcgt
    24601 cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt
    24661 cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa
    24721 tgcaagccca ccttgcccat tggcgcaagc tactctcgtt tcagtgactt tcattacgtt
    24781 tcttttgtga atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac
    24841 gcagtatcaa ttgaaacaaa acgaaagatc aatatcgcag taatctgaac tggagaggaa
    24901 agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atgcaattta
    24961 tgccgtttgt tccgcacatc cgctggtgct ggaggctgca atccgctacg ccagtgcaaa
    25021 ccaaacgccg ttactgattg aagcaacctc caatcaggta gaccagttcg gcggttatac
    25081 cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt
    25141 cccgcaggat gcgctgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct
    25201 gccagccgct caggcaatgg ccaatgccga tgatttgatt aaaagctacg ttgcggcagg
    25261 attcaaaaaa atccaccttg attgcagcat gtcctgtcag gacgatccaa ttcccttaac
    25321 tgatgacatc gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg
    25381 cgaacacttt ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg
    25441 cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac
    25501 gctggaagcc catcgtcacg cctttgaaaa gcaaggtttg aatgccatct ggccacgcat
    25561 tattgccctg gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca
    25621 gcccgccaaa gcgagcgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga
    25681 agcgcactct accgattatc aaacgccgca atctctgcgc cagctggtga ttgaccactt
    25741 tgccattctg aaagttggcc cggcgctgac cttcgccctg cgtgaagccc ttttttctct
    25801 ggcggcgatt gaagaagaac tggtaccagc gaaagcctgt tccggtctgc gtcaggtgct
    25861 ggaaaacgtg atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa
    25921 cgcgcgtcgt ctggcgcgtg gttacagcta ctcagatcgc gtgcgctatt actggccgga
    25981 cagccagatt gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc
    26041 gctaccgctc atcagccagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct
    26101 gcaaccgacg ccacgggaac tcattataaa ccatattcag gacatcctgg cgcagtacca
    26161 cacagcctgt gaaggccaat aagcaaaaca aagaggaaca tgctatgcca aatattgttt
    26221 taagccggat tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg
    26281 cgggggcaaa tctggtgctg gtagccaacg atgaggttgc cgaagatccg gtacaacaaa
    26341 acctgatgga aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag
    26401 ttatcgacaa cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac
    26461 ccgccgattt cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca
    26521 atatgcacta cgccaatggc aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg
    26581 atatcgcagc atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc
    26641 cgacagagcc tgctgtggac ctctttaaat tactttgagg gattcatcat ggaaatcagc
    26701 ctgttgcagg catttgcgtt gggcattatc gcctttatcg ctggcctaga tatgtttaac
    26761 ggcttaaccc atatgcaccg cccggtggtc ctcggcccgt tggtcgggct ggtacttggc
    26821 gatctgcata ccggaatttt aaccggcgat acgctggaac tagtgtggat ggggctggcc
    26881 ccgctggcgg gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt
    26941 gccattacta ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc
    27001 gcagtacaga tggggattac cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc
    27061 gaccggatgg cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcactg
    27121 ctggcactcg gcactttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa
    27181 catgcaaaaa ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg
    27241 ggcggcatca tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc
    27301 tacataccct acttcatcct gggctttgtt gccgcaacct ggctcaagtt accggtgctg
    27361 gctattgctg ccgccgcgct ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa
    27421 ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct
    27481 gccgaacgtc agcgaaaatg aagaaacact gctgactggc gtcaatgaaa acgtgtatga
    27541 agatcggagc attgccgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc
    27601 catgctgtta caagcgtctt ttaactacga acgtatgcag gcttctggct ggttgtacgg
    27661 tctgctgcct gctctgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa
    27721 ggggcatatg ggtttcttca atacccatcc gtttctggtg acgtttgtta tcggcattat
    27781 ccttgcgatg gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt
    27841 cggtgcgccg cttggcggga ttggcgatgc gatgttctgg ctaacgctac tgccgatttg
    27901 tggcgggata ggcgcaagcc tcgcgctaca aggctctatt cttggcgcag tcgtctttat
    27961 tgtgctgttc aacgtggtac acttggggct gcgttttggt ctggcgcatt atgcttaccg
    28021 catgggcgtg gcggcgattc cccttattaa agcgaatacc aaaaaagtcg gccatgctgc
    28081 gtcaatcgtt ggaatgacgg taatcggcgc actggtggca acctatgttc gtttaagcac
    28141 cacgctggaa atcacggcgg gcgacgcagt agttaagtta caggctgatg ttatcgacaa
    28201 actgatgcct gccttcttac cgctggtcta caccctgacc atgttctggc tggtacgccg
    28261 cggctagagt ccgctgcgcc tgattgcggt taccgtggtt ctagggatcg tcggtaaatt
    28321 ctgccatttc ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat
    28381 ggcgggtttg ccagtggcat ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag
    28441 tttattgcca tcgattttcc cgaaacctca agcgccgcgc tgcttacatc gcagcttgaa
    28501 gaggccatcg cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc
    28561 ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt
    28621 accggcacca atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt
    28681 gaagaattcc gtgtacaggc gctggagtgt ggacaccgag ggctgaccag tctggtggac
    28741 gagttaggcc gctgccatga agaatgtccg gtcgaggaag gaatatgaca cacgttctgc
    28801 gcgccagaag gctgctgact gaagagggat ggttcgatga ccatcagttg cgtgttgctg
    28861 acggtgtcat cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac
    28921 tgctctgccc cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg
    28981 atgacgcgcc ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca
    29041 gttggttacc gaccaccgta accgcgccgc ttaataccat tcatgcggcg ctgaaacgta
    29101 ttgctcaacg ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag
    29161 gaccgtactt cacgccgcag aataaaggcg cgcatccgcc
//
LOCUS       DAEEDS010000048        29051 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEEDS010000048 DAEEDS010000000
VERSION     DAEEDS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29051
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.048"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="KLH32_002330"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="KLH32_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBE0139130.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3188
                     /gene="mdtO"
                     /locus_tag="KLH32_002331"
     CDS             1140..3188
                     /gene="mdtO"
                     /locus_tag="KLH32_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBE0139131.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3185..4651
                     /gene="mdtP"
                     /locus_tag="KLH32_002332"
     CDS             3185..4651
                     /gene="mdtP"
                     /locus_tag="KLH32_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBE0139132.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4849..5088
                     /locus_tag="KLH32_002333"
     CDS             4849..5088
                     /locus_tag="KLH32_002333"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139133.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5098..5268
                     /locus_tag="KLH32_002334"
     CDS             5098..5268
                     /locus_tag="KLH32_002334"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBE0139134.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5317..6729
                     /gene="fdhF"
                     /locus_tag="KLH32_002335"
     CDS             5317..6729
                     /gene="fdhF"
                     /locus_tag="KLH32_002335"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBE0139135.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VESYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6730..6996
                     /locus_tag="KLH32_002336"
     CDS             6730..6996
                     /locus_tag="KLH32_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBE0139136.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7090..7779
                     /locus_tag="KLH32_002337"
     CDS             7090..7779
                     /locus_tag="KLH32_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE0139137.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="KLH32_002338"
     CDS             complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="KLH32_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBE0139138.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="KLH32_002339"
     CDS             complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="KLH32_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBE0139139.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="KLH32_002340"
     CDS             complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="KLH32_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBE0139140.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10481..12199)
                     /locus_tag="KLH32_002341"
     CDS             complement(10481..12199)
                     /locus_tag="KLH32_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBE0139141.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="KLH32_002342"
     CDS             complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="KLH32_002342"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBE0139142.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="KLH32_002343"
     CDS             complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="KLH32_002343"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBE0139143.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="KLH32_002344"
     CDS             complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="KLH32_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBE0139144.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="KLH32_002345"
     CDS             complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="KLH32_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBE0139145.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16280..18238
                     /gene="acs"
                     /locus_tag="KLH32_002346"
     CDS             16280..18238
                     /gene="acs"
                     /locus_tag="KLH32_002346"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBE0139146.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18438..18752
                     /locus_tag="KLH32_002347"
     CDS             18438..18752
                     /locus_tag="KLH32_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBE0139147.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18749..20398
                     /gene="actP"
                     /locus_tag="KLH32_002348"
     CDS             18749..20398
                     /gene="actP"
                     /locus_tag="KLH32_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBE0139148.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20576..21868
                     /locus_tag="KLH32_002349"
     CDS             20576..21868
                     /locus_tag="KLH32_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE0139149.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22022..23671)
                     /locus_tag="KLH32_002350"
     CDS             complement(22022..23671)
                     /locus_tag="KLH32_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBE0139150.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="KLH32_002351"
                     /gene_synonym="yjcD"
     CDS             complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="KLH32_002351"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBE0139151.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="KLH32_002352"
     CDS             complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="KLH32_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBE0139152.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26427..26750
                     /gene="soxS"
                     /locus_tag="KLH32_002353"
     CDS             26427..26750
                     /gene="soxS"
                     /locus_tag="KLH32_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBE0139153.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="KLH32_002354"
                     /pseudo
     CDS             complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="KLH32_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28759..29040
                     /locus_tag="KLH32_002355"
     CDS             28759..29040
                     /locus_tag="KLH32_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBE0139154.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7289 a         7779 c         7349 g         6634 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gctcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtatcggt attcagtcga acgcgtttta caccaaattt gtggcgatct
     1381 tgtttgtggt tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat
     1441 acggcgaacc gttgatccga ttgatcatcg ccgggccgat cctgatgggc tgcatgtttt
     1501 tgatgcgcac ccatcggctg ggactggtct ttttcgctgt cgccatttac gggcaaacct
     1561 tccccgccat gctcgactat ccggaagtag tcgtgcgctt aacgctgtgg tgtatcgttg
     1621 ttggcctcta tccaaccttg ctgatgacgt taatcggcgt gctgtggttt cccaatcgtg
     1681 ccattacgca aatgcatcaa gcgcttaatg atcggcttga tgatgccatt agccacctga
     1741 cggacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg gcgctgcaaa
     1801 aactgaatgt cttttgtctc gcggacgatg ccaactggcg aactcaaagc gcatggtggc
     1861 aaagctgcgt ggcaacggta acctacattt actcgacgct gaatcgctac gatcccacct
     1921 cttttgctga ttctcaggca attattgaat ttcggcaaaa attagcttca gaaatcaaca
     1981 agctgcagca tgccgttgct gaaggtcagt gctggcaaag cgactggcgg atcagtgaaa
     2041 gtgaagcggt ggcggcacgg gaatgtaacc tggagaatat ctgccagacg ttgttacaac
     2101 tgggccagat gaacccgaat acgccgccaa cgcccgccgc caaaccgcca tcaatggtcg
     2161 ccgatgcttt taccaatcca gactatatac gctacgcggt aaaaacattg ctcgcctgtt
     2221 tgatctgtta caccttttac agcggcgtgg actgggaagg cattcacacc tgtatgctga
     2281 cctgcgtgat cgtcgctaat ccaaatgtcg gttcgtcgta ccagaagatg gtgctgcgtt
     2341 ttggcggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg gtcatgccct
     2401 ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg ttggccgcat
     2461 ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg gtcaccttcg
     2521 cgctcgccac gctcgaaaac gtttttggcc cagtgtacga cctggtggaa attcgcgatc
     2581 gcgccctggg tatcatcatt ggtaccgtgg tgtccgcgat gatttacacc tttgtctggc
     2641 ctgaaagtga agcgcgcacg ctgccgcaaa aactggctgg cgcgctgggt atgttaagta
     2701 aagtaatgcg gatcccacgc cagcaggaag tcacggctct gcgcacttat ctgcaaattc
     2761 gtatcggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc gtggcgctgg
     2821 agcgtcaact ggacagcgaa gaacgcgcct tactgattga acgttcgcaa acggttattc
     2881 gtcagggccg cgatatcctt cacgcctggg atgccacctg gaattcggcg caggcgctgg
     2941 ataacgcact acagccggac agagcaggtc agtttgccga cgccctggag aaatacgctg
     3001 ccggtctggc aaccgcactc agccgttctc ctcaaataac gcttgaagag acacccacct
     3061 cgcaggccat cctgcccacc ttattaaaac aggagcaaca cgtctgccag cttttcgccc
     3121 gcttgccaga ctggacagcc ccggcattaa cgcccgccac ggaacaggca caaggagcca
     3181 cgcaatgatc aatcgtcaac tttcacgtct gctgttgtgc agcattctcg gcagcacgac
     3241 gctgatttcc ggctgtgccc tggtacgtaa ggattctgcg cctcatcaac agctcaaacc
     3301 ggaacaaatc aaactggccg atgatattca tcttgccagc tccggctggc cgcaggcgca
     3361 gtggtggaaa caactcaatg acccgcagtt ggatgccctg atccaacgga cgctaagtgg
     3421 ttcacacacc ctcgccgaag cgaaactgcg ggaagaaaaa gcgcaatcgc aggccgattt
     3481 gttagatgcc ggttcacagt tgcaggtggc agcgttaggg atgcttaacc gccaacgtgt
     3541 ctcggcgaac ggctttttaa gcccttatgc gatggatgcg cccgcactgg ggatggacgg
     3601 gccgtactat acggaagcca cagtaggttt gtttgccgga ctggatcttg atttgtgggg
     3661 tgtgcatcgc tcagcggttg ccgccgccat tggcgcgcat aatgccgcgc tggcagaaac
     3721 cgcagcagta gagctatcgc tgaccacggg cgtagcgcag ctttattaca gtatgcaggc
     3781 cagctatcag atgctcgatc tgttagaaca aactcgcgat gtgattgatt acgcggtgaa
     3841 agcgcaccaa agtaaagtgg cgcacggtct ggaagcacaa gtgcctttcc acggcgcgcg
     3901 ggcacagatt ctggcggtcg ataaacaaat tgctgccgtc aaagggcaaa tcaccgaaac
     3961 gagagaatct ctgcgcgcat tgattggcgc gggagccagc gatatgccgg agatcaaacc
     4021 tgtggcatta ccacgagtcc agaccggcat tccggcgaca ctctcttatg agttgctcgc
     4081 cagacgcccg gatctgcaag ccatgcgctg gtatgttcag gcgtcattag atcaggtgga
     4141 ttccgcgcgg gcgttgttct atccgagctt tgatatcaaa gcgtttttcg gccttgatgc
     4201 catccacctg gataccttat tcaaaaaaac cagtcgccag ttcaacttca tcccgggtct
     4261 gaaattgccg ctgtttgacg gtggacggtt gaatgccaat ctcgaaggca cgcgcgccgc
     4321 cagcaacatg atgattgaac gttacaacca gtcagtactg aacgcggtgc gtgacgttgc
     4381 cgtcaacggc acgcgtctgc aaacgctcaa cgacgagcga gaaatgcagg ctgaacacgt
     4441 ggaagcaacg cgcttcaccc agcgcgctgc cgaggccgcc tatcagcgcg gcttaaccag
     4501 ccgcttacag gccactgaag cccggttgcc agtacttgcc gaagagatgt cattactgat
     4561 gctggacagc cgccgggtga tccaaagcat tcagttgatg aaatcgctgg gcggcgggta
     4621 tcaggcggct cccgtcgtcg agaaaaaata aaatgtctgc cgcgtgatgg ctgtcacgcg
     4681 gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt tttcgtcaga gttttgaata
     4741 aatagtgccc gtaatatcag ggaatgaccc caaataaaat gtggcataaa agatgcatac
     4801 tgtagtcgag agcgcgtatg cgtgatttga ttaactggag cgagaccgat gaaaaaagtc
     4861 gtcacggttt gcccctattg cgcatcaggt tgcaaaatca acctggtcgt cgataacggc
     4921 aaaatcgtcc gggcggaggc agcgcagggg aaaaccaacc agggtaccct gtgtctgaag
     4981 ggttattatg gctgggattt cattaacgat acccagatcc tgaccccgcg cctgaaaacc
     5041 cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg tttcctgaga tgaggcactg
     5101 aattacgttg ccgagcgcct gagcgccatc aaagagaagt acggtccgga tgccatccag
     5161 acgaccggct cctcgcgtgg tacgggtaac gaaaccaact atgtaatgca aaaatttgcg
     5221 cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc gtgtctgaca cggcccatcg
     5281 gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga gcaatgctat taacgaaatt
     5341 gataataccg atttagtgtt cgttttcggg tacaacccgg cggattccca cccaatcgtg
     5401 gcgaatcacg taattaacgc taaacgtaac ggggcgaaaa ttatcgtctg cgatccgcgc
     5461 aaaattgaaa ccgcgcgcat tgctgacatg cacattgcac tgaaaaacgg ctcgaacatc
     5521 gcgctgttga atgcgatggg ccatgtcatt attgaagaaa atctgtacga caaagcgttc
     5581 gtcgcttccc gtacagaagg ctttgaagag tatcgtaaaa tcgttgaaag ctacacgccg
     5641 gagtcggttg aagatatcac cggtgtcagc gccagtgaga ttcgtcaggc ggcacggatg
     5701 tatgcccagg cgaaaagcgc cgccatcctg tggggcatgg gtgtaaccca gttctaccag
     5761 ggcgtggaaa ccgtgcgttc tctgaccagc ctcgcgatgc tgaccagtaa cctcggtaag
     5821 ccgcatgcgg gtgtaaaccc ggttcgtggt cagaacaacg tgcagggtgc ctgcgatatg
     5881 ggcgcgctgc cggatacgta tccgggatac cagtacgtga aagatccggc taaccgcgaa
     5941 aaattcgcca aagcctgggg cgtggaaagc ctgcctgctc ataccggcta tcgcatcagc
     6001 gagctgccgc accgcgcagc gcatggcgaa gtgcgtgccg cgtacattat gggcgaagat
     6061 ccgctgcaaa ctgatgcgga gctgtcggca gtacgtaaag cctttgaaga tctggaactg
     6121 gtcatcgttc aggacatctt tataaccaaa accgcgtcgg cggcggatgt cattttgccg
     6181 tcaacgtcgt ggggcgagca tgaaggcgtg tttactgcgg ctgaccgtgg cttccagcgt
     6241 ttcttcaaag cagttgaacc gaaatgggat ctgaaaacgg actggcaaat catcagtgaa
     6301 atcgccaccc gtatgggtta tccgatgcac tacaacaaca cccaggagat ctgggatgag
     6361 ttgcgtcatc tgtgcccgga tttctacggt gcgacttacg agaaaatggg cgaactgggc
     6421 ttcattcagt ggccttgccg cgatacttca gatgccgatc aggggacttc ttatctgttt
     6481 aaagagaagt ttgatacccc gaacggtctg gcgcagttct tcacctgcga ctgggtagcg
     6541 ccaatcgaca aactcaccga cgagtacccg atggtactgt caacggtgcg tgaagttggt
     6601 cactactctt gccgttcgat gaccggtaac tgtgcggcac tggcggcgct ggctgatgaa
     6661 cctggctacg cacaaatcaa taccgaagac gccaaacgtc tgggtattga agatgaggca
     6721 ttggtttgag tgcactcgcg taaaggcaaa attatcaccc gtgcgcaggt cagcgatcgt
     6781 ccgaacaaag gggcgattta catgacctac cagtggtgga ttggtgcctg taacgagctg
     6841 gttaccgaaa gcttaagccc gattacgaaa acgccggaat acaaatactg cgccgtgcgc
     6901 gtcgagccga tctccgatca gcgcgccgcc gagcagtacg tgattgacga gtacaacaag
     6961 ttgaaaactc gcctgcgcga agcggcactg gcgtaataca gtcctttcta cagcctcctt
     7021 tcggaggctg tttttttatc cattcgaact ctttatactg gttactccct acccaatcgt
     7081 attatcaaaa tgaaaaaaat tatcgcattg atgttgtttt tgacattctt tgcccacgcg
     7141 aacgactccg agcctggcag ccagtattta aaggcagcag aggccgggga ccgacgtgca
     7201 caatattttc ttgccgacag ctggtttagc tccggcgatt tgagcaaagc cgaatattgg
     7261 gcacagaaag ccgccgacag cggtgatgct gatgcctgcg cgctgttggc gcagatcaaa
     7321 atcaccaatc cggtcagtct ggactatcca caagcaaaag ttcttgcaga gaaagcggcg
     7381 caagcgggca gtaaagaagg tgaagtaacg ctggcgcata ttctggtaaa tactcaagcg
     7441 ggtaaaccgg attatccaaa ggcgatttcg ctgttagaaa acgcctcgga agatctggag
     7501 aatgactccg ccgtcgatgc ccaaatgctg cttggtttga tttacgccaa cggcgtgggc
     7561 attaaggccg acgatgacaa ggcaacctgg tatttcaaac gcagctctgc aatttcccga
     7621 accggttatt ccgagtactg ggcgggaatg atgttcttaa acggtgagga aggttttatc
     7681 gagaagaaca aacaaaaggc gctgcactgg ttgaacctga gctgtatgga agggtttgat
     7741 accgggtgtg aagagtttga aaaattaacc aacggttaag gtaggcctga taagacgcgg
     7801 caagcgtcgc atcaggcatc cacacattgc cgggggatat ccccggcaat cttcaattat
     7861 tgatccgcag ttttatcaaa tttgcccagc acttcacgct cataagccag cgcctttttg
     7921 cggtcaaatt tgtgttccca cttggcaatc accagcaccg ccagcgcatt acccaccacg
     7981 ttcagcgcag tacgcgccat gtcgaggata cggtcaacac cagcaataaa cgccagacct
     8041 tccagcggaa tacctacgct acccagcgtt gccagcaaca ccacaaacga cacgccagcc
     8101 acgccagcaa tccctttcga ggtcaccatc agcgtcagca ccagaatgat ttcctgccag
     8161 atggacaggt caatgccata cagctgagcg atgaaaatag cggcaatact ttgatacagc
     8221 gtcgaaccat caaggttaaa agagtaaccg gtcggcacca cgaagctggt gatcgacgcc
     8281 ggtgctccgt aggcttccat cttctcaata attcgcggca gtacgctttc agagctggca
     8341 gtggagtacg ccagaatcag ctcatctttc agaatacgaa tcaggatcca gacgcttaac
     8401 ccgcacagac gtgccacaat tcccagcact accagcgcga agaacagaat ggcgaaatgc
     8461 accagcagca ccagtttcgc cagtggccac agagacgaga aaccaaagtt agccaccgtc
     8521 accgcaatca gcgcaaacac acccaccggt gcataacgca tcaccatgtg agtcacttta
     8581 aacatggttt cagagatgga gcggaacacg gtcaccagcg gttcacgatg cgtcgcgggc
     8641 agggaagaaa gccccagacc aaacagcacc gagaaaaaga tgatcggcag catttcgcct
     8701 ttcgccatcg acgccacaat gttcgtcggc accagcgaca aaatcgtgcc cataatgccg
     8761 tgggaactgc tttgtaccgc ttccgtagtg ctctgatatt tcgagatatc gacggtcgcc
     8821 agctgcgaca tatccacccc ggcaccgggc tggaagacgt tcgccagagt gatccccaaa
     8881 atgatggcga cggtggtgat cacctcgaag taaataatgg ttttcgcgcc aatacgcccg
     8941 agctgtttgg catcaccaac acccgcgata cccaccacca gcgtggagat cacaatcggc
     9001 acaacaatca ttttgatcag atggatgaag atatcacccg ccggagagag caaattgacg
     9061 accagccagt cgcggctgtc gctatggtag tgcaggtagc ttcccaggag aatgcccagc
     9121 accatagcaa acagaatctg ccaggccagg ctgaatttta tatttttcat cagaataact
     9181 tcctcaatgg agcgccgttc cccgttcaga aacgcgagaa cgtgacatac tgccagggca
     9241 tgaattgtgt tgcgttggtt tgtagatttt tttaacgctt atgatcagta tcatttgcgc
     9301 ggtatgttgc gcaacccttt aacaacagag cttttcgctg cattaatgca taataacgcg
     9361 agtttatggc aggattaatg aataaccatt tattatggaa agatttttat ttgtttaatc
     9421 tgcaataata ctgattggtg attatttttg gcatatgttc attcgattat tttttatgca
     9481 gctcaatgac ataatcttca tcaccagatg attaatccga tctccgctgc aacagttttg
     9541 ccatattaat cgactcgacc agctgtgtcc ggttgatccg gggtgagttg agatccatca
     9601 ctttttgcca tagttcgatg gcttgcgcgt aattcgcctg cataaacgca tcggaagcca
     9661 gcagcatcag agcggtgatt tcgttagagt ccagcgcgag ggctttgtcg atcattgcgc
     9721 gagtctgggc ggtcatatgc tggctggcct ggtaataaag caccgtcgcc agcgccgcat
     9781 acagttcagc gttctcacca cgtagttgca acgcctgacg atacgccagc agcgaattgc
     9841 tgtaatcgtt ttgccacaga tagtactcgc ccagtaacgc ccattgttcg ctgttttgtg
     9901 gattagcgcg gattttatcc tgcaatgcct gaagctgcgc ttccggggtt tgctggctgg
     9961 caaactgatg tagcggatcg cgctgacgct gatactccgc acgtaccgcc tgccattttg
    10021 ggcttaacag ataactgccg acacagagga ataccatcaa tatcgtgagt gttgtcagca
    10081 ttttcaccgg tatttgaggc tgtttcatcg cttagccctc actcgccaga ggatcagtgc
    10141 catcagcaat aacaacacca ctggcagcgc ccacaacacc agcgtctgac ccgttaacgg
    10201 tgggttatag cgaacaaaat ctccgtagcg ttcggtcatc cagccaatga tttcgacttc
    10261 gttcttcccc tccgacacca tgctataaac ctgatggcgc atactgaccg ccaccggcgc
    10321 attggattcg agtaagtttt gattctggca ctgcggacaa cgtaactggc tggcaatatt
    10381 taacgcctgt tgctgttgtt gcggattggc gaattgccag gtgtctacaa cctgagcgcg
    10441 cgcaaaacag gtaaataaca gcagtagcgt gagaagccct ttattcatcg cgcttcttcc
    10501 cccgccatcc gcttaacaat gcgcccgcaa tcatcaacaa tcctcccccc cagatccagc
    10561 gcacaccgct ttgtacatac aaacgaaaag cgtaacgatc cgctctggtt ttttcaccca
    10621 tgaccgcata ccagtcatgg atgccgttcc agcgaattga cggttccatc atttgctgac
    10681 ggcgtgcttc ataaaaacgc cgctccggcg ttaattcacc aatgcgttgc tgatggtcaa
    10741 acagcgccac tatcgctttt tcgctggtgt aattgcctct ggcttgcaga tcgagtcgct
    10801 caaaacggaa ggtgtatcct gccagcgtca cctgctgacc cggctgtaaa ttcaggctga
    10861 tctcctgacg actgacactg gaaaccacga tccccgcggc aaataacagc acgccagcat
    10921 gagctaccag cgccggaagc tgcacgcgtt tgccagagac aaacgtcgcc agcacaatca
    10981 ccaccagcat caacagacca aacggtaacg tcgcgcggtt aaaatacggc gcgccaacgg
    11041 agaggcgtcc ccagcccagc aggccgtaaa tcatcggata aagcgttccc accagcacga
    11101 tcagtagcac tgcgcaaaac agcaacagcg tagcgaggat taacatttcc cgcgataacc
    11161 ccgaaaaacg caccgccggg ccaccgtccc gcgctcgcca gccatacaga gccaaagacg
    11221 caaggctaat cagtgcaaac aggctgaaca acggcacggc gcggacgtta tccagtgcga
    11281 acgcatgaac cgaaaccaga atgccagaac ggacaattaa ggtgcccagc agcgacagca
    11341 tcagagtaac tatcgccagc aacagcgacc agtggcggaa aatcccccgc tggcgtgtca
    11401 gggacaaact gtgcagcagc gcagtggcag aaagccaggg taataaagag gcgttttcca
    11461 ccggatccca gaaccaccag ccgccccagc cgagttcgca gtaggcccac caggaaccga
    11521 ggatgatccc cgccgttaat gcactccagc caggtaacgc ccagcgccag caaattcggg
    11581 cgctggccgc atcaaactcg ccgcgcagta gactcgccag cgccacgctc gccgctacca
    11641 tcaaaccgcc atagccaagg caaagcagcg gtggatgaaa gataagaccg ggatgttgca
    11701 gcatcggatt gagatcgcgg ccttcgattg ctggtggaaa tatccgcacg aagggatcgg
    11761 accacagcac cacaaacagt agcagtgcgg cgagcatgag agataaaacg gctagcgtca
    11821 gcggaaatag cggatcgctt tgctgccgat aatgccaggc aaacagcgcg ctccaggcgg
    11881 aaagcagcag cacccaaagc agcagcgaac cttcatgacc gccccacacc gccgccagtt
    11941 tgagttccca cgacagcagg ctgtagctat gttgggcgac ataaatgacc gagaaatcgc
    12001 tgatgagaaa acaatacgtc agtacgccaa aggcgagcag caggagcgcg aactgcgcca
    12061 gaatgccgat gcaagtgagt cgcatcatgg caggccagcg caaccgcact cccgcaaagg
    12121 ccgtcaacgg ggtcaacacg ttgaccccga gacttaacaa caacgccaga aaaccgactt
    12181 caggaaggaa tacgtccaga aaccacctct tttctggctt acgccaccgt caactgtccg
    12241 gcgtagagaa tgaaaaagcg cagcatcaac acgccgacca gactggcccc acacgccgcc
    12301 agcacggcag gaatgccgga actgcgattg acccacggtt tgagcaacat tggcacaatc
    12361 agccccagcc cggcgacacc aagccagaac caccacgtcc agaaaccgcc acctaatgcc
    12421 gccaccagcg cacgaacttt accgtcatcg cccagcgcca gaccgacaaa aaacgccacc
    12481 agcaggaaga tttcacccca taccacgggg atttccatcc ggtgaacaaa gtgcgcttcc
    12541 gtggaatgcg ggttactgcg ttggcgtatc gccatggcga tcagcgccac cgccgcaccg
    12601 gacgagatgc cggagaagag gagcagcacc ggcaggctcg ggttattgag gaacggatac
    12661 gatttcagcg ccgacagcag aaagccggta taagccccca gcagcaccgc caacaccagc
    12721 atcagcgttt ccagtccgcg atgaacgggc gtcagcaggc tcagaacctt ttgcacgatc
    12781 cccagcttcg gcaaccagcg ttgttgcagg gcaagcaaat catgttcaaa gattttcgcc
    12841 agccacagca ccagcaccac catgtagagc tgaaacagca tcacccccat cgacatcacc
    12901 gaggtaaaac tgtagtggaa catcagcttc cagaaggtcc acggtcttgt caggtggaag
    12961 acgaggatca acagaccgag gatcaccgcg cccggcccga caatcagcgt ggtgcgcagc
    13021 aacgtactgt ctgcaccgcc cgcctgcggg tagaagcgac gtaacagcac ggccagcgtc
    13081 accagaccgg cagaaatacc aatcaaaaac aggtagatgg caatcggcca gtcccacacc
    13141 agcgattcaa aatgaaatgc ggaagtctgc gtcattggct cacctccccg tatttaaacg
    13201 gtacgcggta cagcttcggt ttggttccca gcgccagctt gtagcggtaa gtaggcttct
    13261 ggcgcagcag ttgcgaaatc tcactgttag gatcgtccag attgccaaac gtcagcgcct
    13321 tcgtcgggca agcttcaacg cacgcgggaa gcttaccggc ctgcaaattg gtcttacgac
    13381 agaaatcgca tttgtccgcc gtcttcgtga ccggatggat aaagcgcacg cggtacggac
    13441 aggcggcgat gcagtactga caaccgacgc agagatcagg attaacatcg acaatcccgc
    13501 tagcggcatc acgaaaagac gcaccggtcg ggcagacgtc aacgcacggc acatgatcgc
    13561 agtgctggca agacttacgg aagaagcgat atttcacatc aggaaattcg ccctgcggct
    13621 cgctacgaat aatcgtcagg cgcgagacgc cttccggcac cttgttcact tcccgacaag
    13681 catccataca ggcggtacag ccgatgcata acgactcgtc atgcaccatg ccgtaacgca
    13741 ccccattgat attcaacgtt ttcgccacca cgcgccctgc ggtgccgctg acggctgcca
    13801 gcacgccgac gccggtgaga aactggcgac gagaccaggt catggctgct ccttaagcaa
    13861 cggaacagac gccgggttaa agttcggatt ggtgcgctga tcgctgtggc aatcgacgca
    13921 aatcttaatc cgtcctttgt cacttaacgt ctgcatcgta tcttgctgtg gatgcaggga
    13981 atggcagctg gcgcacgcca ctttggttac gtggacatcg tgcggccaga acgctttttg
    14041 caactgttcc ggcagatgac aggacataca gacgctgttc tgctctccga ccttatacat
    14101 cggctcgtta aagcgcatca catctttcac cccttcgcgg tgttgcggtg atgcttggcc
    14161 gtgacagttg gtgcaggtga ccggcaattt gttattcggg ttgataacgg aagcatgttt
    14221 gccatgcata ccttcggtat ctggtttatg acagtccaga caggcggcat ccgggttacg
    14281 ctgctgggta acttcgtagc gatcaccaga tgcctgcacc gcaggggtag cggtaattcc
    14341 gttcaggctc cacaacaggc ccgacgccag caccccagca gttaataacg aacgtaatac
    14401 gctcatattc actccattgt gttttccgcc ccttgcgagg cggaacgggg ttattggctt
    14461 aacagaccgt ttttacgtgc ctgctcttcc cactgcggga tcaccgtttt gatgaagtcc
    14521 tgcttctcgg ccttgatttg ttccatgttc aggccaatgg cctgctgggc tttctctttg
    14581 gtcgagatat ccgggatctg gatttcatgg gtgatacctt tggtcgccag caggcgcgcc
    14641 agtttggtgc gcgcatccgc cgctttatcc atcgccgtac cgagcatccg tagaccttct
    14701 tccggtgcgt gcatatgaat accgtgggaa gcgatcgcca gatcccaacg ccactgggca
    14761 tgacggatat cgtcctgaat cggcttcatt tcggcttccg tcgcgcctgc atccagcgcc
    14821 gctttcgctt cgaagtgagc gtgaaccagt tgatcttcaa ccttgatttt caggtcgtta
    14881 atcgattgct tacgttccgc gaccactttt tgcaaggcag ctttatcctg ggtatggcag
    14941 ttcgcacaag tctgggcgaa gttatcaaac ggattaccaa ttttatggtc ggtgtagagt
    15001 ttgccttcgg cgttctgcac ttttggcata tggcagtcga tacaggtcac gttgttttta
    15061 ccgtgaatgc ccgctgtcca ggtttcatat tccgggtgct gcgctttcag cattggcgtt
    15121 ttcgacaggg agttagtcca gtcagagaag gcaattttgt cgtaatactg ctccatattt
    15181 tcgactttca tgccgtcatc ccacgggaat ttaaccgctt tgtttttgcc gtcgaagtaa
    15241 tactccacat ggcactgacc gcaaaccatc gattgctggt cgaaacgtcc ggctttctca
    15301 aaaggtttac caatggcttc catcgcgcga gccgcatacg gacgggaaag ggttaactcc
    15361 ggtttgcctt tggcgaactc tggtgaggcg gtgttatggc agtcggcaca acctaagttg
    15421 ttgacgattt ccgggccgcc gcgcgcccat ttaccgtgga agtagccatc ttcgccgtct
    15481 ttctggatca gacgcgccac atccgggctt ttacaactcc agcacgccat cggtagcgga
    15541 ccatcttcag cgtttttcgg cgcaccggta cgcagggttt cacgcacatc ggtcacagca
    15601 aaggcatgtc cacgaggctt gttgtaatcg cgcgagaagg gataccccgc ccacaggatc
    15661 accagccgtg gatcttccgc cagggcgtca acacgctctg actgttccga ggtggctttc
    15721 caggagagat attgatcgtg atgctgcggg gcaaaggttt cattcttcgc ttccacagtt
    15781 acaggttttg cgggagctgc cgtttgttca gcgtgaacag aagtgaaaaa gaaaaaagga
    15841 atcaataagc tgaagatacg gcgtgcgttt atttttatcc ttgtcatagg ggcttcatcc
    15901 gaattgcgcc attgttgcaa tggcggtttt tattgttttt cacgacagta accgcaccta
    15961 cactgtcatg acattgctca cccctatgtg taacaaataa ccacactgtg aatgttgtct
    16021 ttaatcaatt gtaagtgcat gtgaaatacc actttagagt tagtcagtat cttcctcttt
    16081 ttcaacagca tgcataactg catgttcctc aaagaattaa tcaacttttg ttggtgacct
    16141 tcaaaaatta ccctgccgtt tatttgcacc attctacttt tgcgtgatct gtcgcccaaa
    16201 tactaaacaa aactgccaat acccctacat ttaacgctta tgccacatat tattaacatc
    16261 ctacaaggag aacaaaagca tgagccaaat tcacaaacac accattcctg ccaacatcgc
    16321 agaccgttgc ctgataaacc ctcagcagta cgaggcgatg tatcaacaat ctattaacgt
    16381 acctgatacc ttctggggcg aacagggaaa aattctcgac tggatcaaac cgtaccagaa
    16441 ggtgaaaaat acctcctttg cccccggtaa tgtgtccatt aaatggtacg aggacggcac
    16501 gctgaatctg gcggcaaact gccttgaccg ccatctgcaa gaaaacggcg atcgcaccgc
    16561 catcatctgg gaaggcgacg acgccagcca gagcaaacat atcagctata aagagctgca
    16621 ccgcgacgtc tgccgcttcg ccaataccct gctcgagctg ggcattaaaa aaggtgatgt
    16681 ggtggcgatt tatatgccga tggtgccgga agccgcggtt gcgatgctgg cctgcgcccg
    16741 cattggcgcg atgcattcgg tgattttcgg cggcttctcg ccggaagccg ttgccgggcg
    16801 cattattgat tccaactcac gactggtgat cacttccgac gaaggcgtgc gcgccgggcg
    16861 tagtattccg ctgaagaaaa acgttgatga cgcactaaaa aacccgaacg tcaccagcgt
    16921 agagcatgtg gtggtactga agcgtactgg cggaaacatc gactggcagg aagggcgcga
    16981 cctgtggtgg cacgacctgg ttgagcaagc cagcgatcag caccaggcgg aagagatgaa
    17041 cgccgaagat ccgctgttta ttctctatac ctccggttct accggaaaac caaaaggcgt
    17101 actgcacact accggcggtt atctggtgta cgcggcgctg acctttaaat atgtctttga
    17161 ttatcatccg ggtgatatct actggtgcac cgccgatgtg ggctgggtta ccggacacag
    17221 ttatttgctg tacggcccgc tggcctgcgg cgcgaccacg ctgatgtttg aaggcgtacc
    17281 gaactggccg acgcctgccc gtatggcgca ggtggtggac aagcatcagg tcaatattct
    17341 ctataccgca cccacggcga tccgcgcgct gatggcggaa ggcgataaag cgattgaagg
    17401 caccgaccgt tcgtcgctac gcattctcgg ttccgtgggc gagccaatta acccggaagc
    17461 gtgggagtgg tactggaaaa aaatcggcaa cgagaaatgt ccggtggtcg atacctggtg
    17521 gcagaccgaa accggcggtt tcatgatcac cccgctgcct ggcgctaccg agctgaaagc
    17581 cggttcggca acacgtccgt tcttcggcgt gcaaccggcg ctggtcgata acgaaggtaa
    17641 cccgctggaa ggggctaccg aaggtagcct ggtgatcacc gactcctggc ctggtcaggc
    17701 gcgtacgctg tttggcgatc acgaacgttt tgagcagacc tatttttcca ccttcaaaaa
    17761 tatgtatttc agcggcgacg gcgcgcgtcg cgatgaagat ggctattact ggatcaccgg
    17821 gcgtgtggac gacgtgctga acgtctccgg tcaccgtctg gggacggcag agatagagtc
    17881 ggcgctggtg gcgcatccga agattgccga agccgccgta gtaggtattc cgcacaatat
    17941 taaaggtcag gcgatctacg cctatgtcac gcttaatcac ggggaggaac cgtcaccaga
    18001 actgtacgca gaagtccgca actgggtgcg taaagagatt ggcccgctgg cgacgccaga
    18061 cgtgctgcac tggaccgact ccctgcctaa aacccgctcc ggcaaaatta tgcgccgtat
    18121 tctgcgcaaa attgcggcgg gcgataccag caacctgggc gatacctcga cgcttgccga
    18181 tcctggcgtg gtcgagaagc tgcttgaaga gaagcaggct atcgcgatgc catcgtaacc
    18241 cacaattgcc ggatgcgaat cggtaacggt ttgtaggcct gataagacgc gacagcgtcg
    18301 catcaggcat tgattgccgg atgcggcgta aacgccttat ccggcctaca ttcggcaagg
    18361 gttacccgag cgttaacctt ctcccataag ggagcgggaa ttaaaacaat ccctacatta
    18421 cctctggaga atctgtgatg aatggtacga tttatcagcg gatagaagac aatgcgcatt
    18481 tcagggagtt agtcgaaaaa cggcaacggt ttgccaccat cctgtcgatt attatgctgg
    18541 cagtttatat cggctttatt ttactgatcg ccttcgcgcc cggctggctg ggcacgccgc
    18601 tgaatccgaa caccagcgtc acacgcggta ttccaattgg tgttggagtg attgtgatct
    18661 cctttgttct caccggtatc tacatctggc gggcgaacgg cgaattcgac cgtcttaata
    18721 atgaagtcct gcatgaggta caagcatcat gaaaagagtt ctgacggcgc ttgccgccac
    18781 actccctttc gcagctaacg ccgcggatgc tattagcggg gccgtagagc gccagccaac
    18841 gaactggcag gcgattatta tgttcctgat tttcgtcgtg tttacgctcg gcattaccta
    18901 ctgggcatca aaacgcgtac gttctcgtag cgactactac accgcaggcg gcaatatcac
    18961 tggcttccag aacgggctgg cgattgccgg ggactatatg tccgccgcct cattcttggg
    19021 gatctccgcg ctggtgttta cctccggcta tgacgggctg atctactcgt tgggcttcct
    19081 ggtgggctgg ccaatcattc tgtttctgat tgccgaacgt ctgcgtaacc tgggacgcta
    19141 cacctctgcc gatgtggcct cttatcgtct gaaacaaggg ccgattcgta ttctttcggc
    19201 ctgtggttct ctggtagtgg tggcgcttta ccttatcgct cagatggtag gcgcaggtaa
    19261 actgatcgag ctgctgtttg gccttaacta tcacattgcg gtggtgctgg tcggcgtgct
    19321 gatgatgatg tacgtcctgt tcggcggcat gctggcgacc acctgggtac aaattatcaa
    19381 agctgtgctg ttgctgttcg gtgccagctt tatggccttt atggtgatga aacacgtcgg
    19441 ctttagcttc aacaatctgt tcagcgaagc gatggcggta cacccgaaag gtgtcgacat
    19501 catgaaaccg ggcggactgg tgaaagatcc gatctccgcg ctctctctgg ggctgggact
    19561 gatgtttggt acggcgggct tgccgcacat tctgatgcgc ttctttacag tcagcgatgc
    19621 ccgcgaagca cgtaagagcg tgttctacgc caccggattt atgggctact tctatattct
    19681 gacctttatt atcggcttcg gcgcgatcat gcttgttggt gcgaatccgg aatataaaga
    19741 cgcggcgggc catctgattg gtggtaacaa catggcggcc gttcacctgg cgaatgcagt
    19801 gggcggcaac ctattcctcg gttttatttc agcggttgct ttcgccacta tcctcgcggt
    19861 ggttgcggat ctgacactgg cgggcgcatc ggcggtttcg catgacttgt acgctaacgt
    19921 cttcaaaaaa ggcgcgaccg aacgtgaaga gctgcgggta tcaaaaatca ccgtactgat
    19981 cctcggcgtg attgcgatta tcctcggcgt gctgtttgag aatcagaaca tcgcctttat
    20041 ggtaggcctt gcgtttgcca tcgcggcgag ctgtaacttc ccgatcattc tgctttccat
    20101 gtactggtcg aaactgacta cccgtggcgc gatgctgggc ggctggctgg ggctgattac
    20161 cgcagtggtg ctgatgatcc tcggcccgac tatttgggta cagatcctcg gtcacgaaaa
    20221 agccatcttc ccgtatgaat acccggcgct gttctctatc agcgtggcat tcctcggcat
    20281 ctggttcttc tcggcaaccg ataactcagc ggaaggggcg cgcgagcgtg aactgttccg
    20341 cgcgcagttt atccgctccc agaccggctt tggcgttgag caaggccgcg cacattaatc
    20401 tctccccttc cccggtcgcc tgaccgggga atacgcttcc tctccaacat gcatcacctt
    20461 ttcccataat attaaacaaa taaactcatt aaaaaatgag cgatttttga cagtcgtaga
    20521 aaatgataat gcagagaata tgccttttct ttcttgttaa ttataaggat attttatgcg
    20581 ctataatggt ttaaataata tgtttttccc tctttgccag attaacgata accactctat
    20641 cacaagttca tcacatacaa agaaaacaaa atcagataat tacagcaaac atcataaaaa
    20701 cacgttaatt gacaataaag ccctctctct tttcaaaata gatgatcatg aaaaagtgat
    20761 aggcttgatt cagaaaatga aaagaattta tgatagttta ccatcaggaa aaatcacgaa
    20821 agaaacggac aggaaaatac ataaatattt tatagatata gcgttatatg caaataataa
    20881 atgtgacgat agaattacga gaagagttta ccttagtaaa gaaaaggatg tatccattaa
    20941 ggtggtatat tttataaata atgtcgccgt ccataataat actatcgaaa ttccacagat
    21001 agtaaatggt ggttacgatt tttcacacct tagcctgaag ggtatcgtga ttaaagatga
    21061 agatttatcc aattcgaatt ttgcaggttg cagactacaa aacgctattt tccaggactg
    21121 taatatgtat aaaacgaatt tttattacgc cataatggaa aaaatacttt ttgataattg
    21181 tattctcgat gactcaaatt tcgctcagat aaaaatggcc gacggaactc taaatgcatg
    21241 ctccgctatg catgttcaat tctacaatgc agcaatgaat agagccaata ttaaaaatac
    21301 cttccttgac tattcaaatt tttatatggc gtacatggct gaggtaaatc tttataaagt
    21361 aatagcgcca tatgttaatt tatttaaagc cgaccttagt ttctctaaac tcgatttaat
    21421 taactttgaa catgctgatc tgtctcgcgt caatctgaac aaagcaatcc tccagaatat
    21481 aaacttaatt gatagcaaac tcttttgtac gtggctgaca aatacattcc tcgaaatggt
    21541 tatatgtacc ggctctaata tggctaatgt taattttaat aatgccaatt taagcaactg
    21601 ccatttcaac tgttctattt taacaaaagc ctgtatgttt aatacccgtc tctatcgggt
    21661 taatttcgat gaggctagcg tccagggaat gggcatttcc attctccgtg gggaggaaaa
    21721 tatccccatt gatagtgata ccctggtaac actacagaaa ttctttgaag aagattgtac
    21781 ctctcatact ggcatgtcac aaactgagga taatattaat gcagtcgcta tgaagattac
    21841 tgcagatatt atgcaacacg cagattgagg ttaagtcgaa gaacagccaa tgaattgact
    21901 ttacaagaga gataacttat ttgttttaga atcatgaatg ttcgcccgag ctaacctgtt
    21961 aaaggtgcaa aagattgcac ctttttcaga ttacctcggg cctggttacg tcgctttcgg
    22021 cttactgatt ttcctcaatt agcaacgctt caagcaaatc gagatcgtgc agtaattttt
    22081 gcagcgtttc gttgctgatc tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag
    22141 aacgcaatgc cgccagacgg aagcgacgct caaggttctc ttcctgcacg gaactttcaa
    22201 cgtcgttacg tccatcggcg cgacgacgca ggttaccaat gacgcgagaa ctgacctccg
    22261 taagcaactg gttatcgata ttctcttcgg tatccgccgc cagacgctcc tccattttct
    22321 ggatcgccac aatcgccact tctgccgttg ccgctcgcgc aatacgttct tctttcaatt
    22381 gttgcgaatg gtccgcgact tcaatgtgtt gtagcagaat aggcaacatc accacgccga
    22441 caaacagcga gaagagaatg acgccagccg ccaggaacac cagctcatag cgcgccggga
    22501 agacgttacc atccggcaag agcagcggga ttgagagcac accggccaga gtgatcgccc
    22561 cacgcacccc agcgaaagac gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca
    22621 tcggcttctt cttcaggaag cggttgctga actttttcat cgtccacagc cagccgaaac
    22681 ggaccagcat cagcgccgca tatatcagaa taatatcggt aaacagcatc cagatttcga
    22741 cgttagggtc gatttctgcc gccatcagcg acgtctccag aatacccggc agttgcagac
    22801 ctaacagcag gaacaccatg ccgttaaaga caaattccag catcgcccag gtgctgtttg
    22861 cgcgcaggcg cattgccagt ggcgcacggc gcatcacacc ggagcgggtg atggtcatcc
    22921 cggcggcaac cgcagcgagg atgcccgaaa cgccaatatg ttcggcaatc agataagaag
    22981 cgaatggcag caagaacagc agaacgatct gcgtcgcggg ttcatcaccg ccccagcggc
    23041 tgaggaatcg cagcgagcga ccatacagcc agctcaccac aaaaccggca agaataccgc
    23101 caatggctac tttcataaat tcgaccgtcg caccgccgac agtgaagatc atcgtcccca
    23161 tcgccactgc cacggcaaac ttcaacgaca ccaggccgga agcgtcattc atcaacgctt
    23221 cgccctgcaa aatgcccatg atttttttcg ggatgcgccc ttcgccaaca atcccggaga
    23281 gcgccacagc atcggtcgga gaaagtaccg ccgccagcgc aaaggcgggg atcagcggaa
    23341 tgcccggcac cacccagtaa ataaggaagc caatgccgac cacggtgacc accaccagcg
    23401 ccagcgcgag gccgaaaatc tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc
    23461 agccatcagc gaacagcaac ggcgggataa acaagactaa aaagagttca ggatcaaact
    23521 ccacatgcaa accaaacgtc ggccacgcca gtagcgcacc gatggcgatt tgcataagcg
    23581 gaagcgggat ctgaaagggc atgacacgag tgaccacccc ggacagcgag accacgaggg
    23641 tcattatcag tatggtgaag aagatttcca tgcgttccct gtttgcgatg ttttttatga
    23701 tctgagaaag acgttatgcc tgaattctca ttttttcaga atatcgcaaa agctcacaca
    23761 atgtgcggag aaaataaaaa cgggcagcca ttgccacccg ttttcagaat ccgatgaaag
    23821 attagatagc ccagccaccc gcatagaagg tcaccagcgc cacggcgata acgaccgtac
    23881 cgatgttcaa cttgcgccat tcaccggaaa ccagacgacc aatcaccaga gtcgcgaagc
    23941 cgatcatgat gcctgttacg atgttacagg tcagcacgat gaataccgcc gtaaccagac
    24001 ccgccatcgc atcaacaaaa tcagcaaagt cgattttcgc cacgttgctc agcatcagca
    24061 ggccaacgta catcagcgcc ggagccgttg cataccccgg aacgaggtaa gaaagcggag
    24121 agaggaacag aatcagcagg aacagcacgc caacggtgat agccgtcaac ccggttttac
    24181 cgcccgccgc cgtacccgcc gcagactcga tgtataccgc tgccggagct gcacccacca
    24241 ggccagagaa aacgctgctc atggagtcag tggtcagtgc tttcccgccg tcgatgatct
    24301 gcccatcttt atccagcagg ttcgcctggc cggcgacagc gcggatagtt ccggtggcat
    24361 caaatactgc cgtcatcacc agcgccagaa cgcttggcag gactacagga ttcagcgcgc
    24421 ccataatgtc caggctgcca atcagtgaat tgccgttttc atcgctcaat gaaggcatgg
    24481 cgaaaacgcc ggagaaatgg acgttaggat cgaagatcaa accgacaatt gagataccga
    24541 taatggtcag cagaatgcca ccagggactt tcagtttttc caagccgatg atcaccgcca
    24601 gacctaccag tgacataatc accgggaagg tcgcgaaatc acccagcgca acgggcagac
    24661 catcaagcgg gtttttaatc accagaccga caccattagc ggcaatgagc agcaggaaca
    24721 gaccgatacc gatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc
    24781 tacggatacc cgtggcagaa attaccgtaa acagcacacc catcaggaac acggcaccca
    24841 gcgcgacagg tacgctaata tgttgcccca gcaccaggct gaatgcggta aacgccgtca
    24901 gggagatggc gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac
    24961 cgagtccggc aaccagacag gttgcaacga aaactgccgc aggcgggaag cctgctttac
    25021 ccaacatacc tggaacgacg atgaccgagt agaccatcgc cagaaacgtt gttaacccgg
    25081 caaccacttc ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg
    25141 aaccgccggt acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt
    25201 cgtcaagatg cgtggcggac aatcccctgc cagcgaaacg ttatcttcct gctccaggtt
    25261 tgcggcaatt tggtgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt
    25321 ggtaaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc gaagtttgct
    25381 gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc gcaaacgtta
    25441 tcgtcgatgt gcaagattgc agcgttatcg agccgtatac cgttctggat tgcgataaaa
    25501 aatcgtctga gatgacaaaa atattgcggc taaaaagtat acttttacac tggcgaagaa
    25561 acgtcggtga catacaatta aagcatcaac accaaccgga acctccacca cgtgctcgaa
    25621 tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgttcca gcgggataga gagaaagaca
    25681 aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgtgtt tgcggataat
    25741 tccaaaagag ctcgatcagt cccctcgccc cttcggggag agggttaggg ttaggggaac
    25801 aggcagcacc agtgcaaata tttaccctca ccccggcccc atagttaacg atgagtcagc
    25861 ttgtccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt
    25921 ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac
    25981 caatacatcc gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc cgatccaact
    26041 cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaacgta tgcccttctg
    26101 gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat gccaatacgc tgagcgattt
    26161 tgataattgc gacatatcgc aacacatcac gtttatatcg ccgctgattg ccgctgttac
    26221 ggatactagt aatcaaccct ttgctttcat agaaatgcag cgccgatacc gccacaccgc
    26281 tgcgtttcgc cacttcgcca ggggttagca gcgctttaat gcggggtaat ttcttttcca
    26341 taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat gaattaacga
    26401 actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag gatcttatcg
    26461 catggattga cgtgcatatt gaccagccgc ttaacattga tgtcgtcgca aaaaaatcag
    26521 gctattcaaa gtggtacttg caacggatgt tccgcacggt gacgcatcag acgcttggcg
    26581 attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc accgagcgtc
    26641 cgatttttga tatcgcaatg gacctgggtt atgtctcgca gcagaccttc tcccgcgttt
    26701 tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtag ttttattgcc
    26761 cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc tgcggcggca
    26821 tcgccttcgc aaaaaaccat ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa
    26881 cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccaggctg tgcgccagct
    26941 cgatgatgtg ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg
    27001 atttatcgat tttcaaaata tcgacattca atgatttaag gttatgcaag ttagagtagc
    27061 caataccaaa atcatcaatt gccacttcgt aaccagcctg gcggaaagcc agaattaatc
    27121 ggcgtcattt tttcgacatc aagaaatgca tgctcagtca cttcaaattt aatttgctgc
    27181 ggacgcaccg cgtattgctc tgttttctga ttgattcgcg ctatcaaccg tgacgtatga
    27241 aaatcggagg ccgacaggtt aatagaaaca tagcgatcgg catgtgttgc caggtacgcg
    27301 cccagatcgc ggaagacatt atcaataaca taatcagtta tctgttctat catcccctcc
    27361 ttttctgcca gcggaataaa ctctgccgga ttcattattt gcccctgctc accaggccaa
    27421 cgtaacaacg cttcagcgcc gatacatttt tctgttttga tatcgattat tggctggtaa
    27481 taaagacaaa gttgatgttt ttcgagggcg cgttgcaatt tacgtttggg agataaatag
    27541 ttttgtcgaa tacgtagcca gagtaatagc agaacaagac tccccaaaat accggcgggc
    27601 aacgcaaaaa taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca
    27661 atggctgcaa ttgggcgttt tgtcgaataa attgtcgcat ataaatagcc atttctttgt
    27721 acggttaaat ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt
    27781 aacgagaaaa aggttttcgt caccgtatca tacacacccc attgcaatgt cgggtcatca
    27841 gacatcactt cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata
    27901 taggtcattt tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta
    27961 ggttcacgct tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat
    28021 gagcataaaa aatggttatc acgaattcat taatataaag atagccacga ataatattca
    28081 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
    28141 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
    28201 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
    28261 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
    28321 gatgactcat tgctatcaat ttcctgcctg tttataccca gccattgcaa ggtgaaaccc
    28381 tggctaccga cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa
    28441 attgatacct ttttaatcgt cacctggatg aaaataattc attgatattc aaaattaatt
    28501 ttctacagga atacagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat
    28561 aatttgtaca gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat
    28621 agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg
    28681 atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtccaggg catcggctct
    28741 tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct tcgctggcaa
    28801 cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt tcgtcggtct
    28861 gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg ttgtttccgc ctgttggttc
    28921 gcaatggggt tgcttggcat cacaatggcg gacatcaccg ctatctggca caacatcgag
    28981 tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat tttgacctga
    29041 tacgtagaag c
//
LOCUS       DAEEDS010000049        28426 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEEDS010000049 DAEEDS010000000
VERSION     DAEEDS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28426
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.049"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            564..1334
                     /locus_tag="KLH32_002356"
     CDS             564..1334
                     /locus_tag="KLH32_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBE0139155.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="KLH32_002357"
     CDS             1325..1798
                     /locus_tag="KLH32_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBE0139156.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="KLH32_002358"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="KLH32_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBE0139157.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="KLH32_002359"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="KLH32_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBE0139158.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="KLH32_002360"
     CDS             3233..3727
                     /locus_tag="KLH32_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBE0139159.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="KLH32_002361"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="KLH32_002361"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBE0139160.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KLH32_002362"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KLH32_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="KLH32_002363"
     CDS             7571..8485
                     /locus_tag="KLH32_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0139161.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="KLH32_002364"
     CDS             8700..10061
                     /locus_tag="KLH32_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139162.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KLH32_002365"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KLH32_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBE0139163.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KLH32_002366"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KLH32_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBE0139164.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="KLH32_002367"
     CDS             12611..13900
                     /locus_tag="KLH32_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBE0139165.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="KLH32_002368"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="KLH32_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBE0139166.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16204
                     /gene="hpaD"
                     /locus_tag="KLH32_002369"
     CDS             15365..16204
                     /gene="hpaD"
                     /locus_tag="KLH32_002369"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBE0139167.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRTI
                     LKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWREGQFK
                     EFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVNAVFP
                     LPA"
     gene            16214..16594
                     /locus_tag="KLH32_002370"
     CDS             16214..16594
                     /locus_tag="KLH32_002370"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBE0139168.1"
                     /translation="MPHFIVECTENIREEARLPGLFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16662..17465
                     /gene="hpaH"
                     /locus_tag="KLH32_002371"
     CDS             16662..17465
                     /gene="hpaH"
                     /locus_tag="KLH32_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBE0139169.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17476..18279
                     /gene="hpaI"
                     /locus_tag="KLH32_002372"
     CDS             17476..18279
                     /gene="hpaI"
                     /locus_tag="KLH32_002372"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBE0139170.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18301..19656
                     /gene="hpaX"
                     /locus_tag="KLH32_002373"
     CDS             18301..19656
                     /gene="hpaX"
                     /locus_tag="KLH32_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBE0139171.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19666..20556
                     /gene="hpaA"
                     /locus_tag="KLH32_002374"
     CDS             19666..20556
                     /gene="hpaA"
                     /locus_tag="KLH32_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBE0139172.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20583..21520
                     /locus_tag="KLH32_002375"
                     /pseudo
     CDS             20583..21520
                     /locus_tag="KLH32_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21747..23305
                     /gene="hpaB"
                     /locus_tag="KLH32_002376"
                     /pseudo
     CDS             21747..23305
                     /gene="hpaB"
                     /locus_tag="KLH32_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23323..23835
                     /locus_tag="KLH32_002377"
     CDS             23323..23835
                     /locus_tag="KLH32_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBE0139173.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24228..26378
                     /gene="btsT"
                     /locus_tag="KLH32_002378"
     CDS             24228..26378
                     /gene="btsT"
                     /locus_tag="KLH32_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBE0139174.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26428..26631
                     /locus_tag="KLH32_002379"
     CDS             26428..26631
                     /locus_tag="KLH32_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBE0139175.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26642..27598
                     /gene="yjiA"
                     /locus_tag="KLH32_002380"
     CDS             26642..27598
                     /gene="yjiA"
                     /locus_tag="KLH32_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBE0139176.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27667..>28426)
                     /locus_tag="KLH32_002381"
     CDS             complement(27667..>28426)
                     /locus_tag="KLH32_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBE0139177.1"
                     /translation="NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMART
                     EDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQ
                     HADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQ
                     ALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSF
                     YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6958 a         7430 c         7122 g         6916 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcac
    15841 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    15901 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    15961 gttcgaccgc cagatggacg agcgtgtggt gaagctgtgg cgcgaaggcc agttcaaaga
    16021 gttttgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16081 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16141 taccgagctg ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgctgcccgc
    16201 gtaaggacgt tttatgccgc actttatcgt ggaatgtact gaaaatattc gtgaagaagc
    16261 ccgcttacct gggctgtttg ccagtgttaa cactgcactg gcggcaacgg ggatttttcc
    16321 gcttggggga atacgtagcc gggcgcactg gatcgatacc tggcaaatgg ctgatggcaa
    16381 gcacgattac gcctttgtgc atatgacgct aaaaatcggc agcggacgca gtctggaaag
    16441 ccgtcaggaa gtgggcgaaa tgctgtttga tctgattaaa acgcacttcg cctcactgat
    16501 ggagagccgt tacctagcgc tttcatttga gattgcagag ctgcacccga cgttgaattt
    16561 caaacagaac aacgtacacg cgttgtttaa ataaatttgc accagcttcc aggccggatg
    16621 ataaggcgct gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacacaccc
    16681 acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga
    16741 tctcgctgga ttacccggag atcaccatcg aagacgccta cgccgtgcag cgcgaatggg
    16801 ttcgcctgaa aatcgccgaa agtcgcacgc tgaaaggcca caaaatcggt ctgacctcga
    16861 aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggtgcactg atggacgaca
    16921 tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag
    16981 tggagctggc ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg
    17041 tttacaacgc cacggactat gtgatcccgg cgctggagct gatcgacgct cgctgccaca
    17101 acatcgatcc ggaaacccag cgcccgcgca aagtattcga caccatttcc gataacgccg
    17161 ccaacgccgg agtgatcctc ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt
    17221 ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg
    17281 tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg
    17341 tacaactgga agccgggcaa atcattctcg gtggttcgtt cacccgcccg gttccggcgc
    17401 gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg
    17461 tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgtccgcag
    17521 attggattat ggctggggct gagcagcagc tacagcgcgg agttactggc cggaccagga
    17581 ttcgactggt tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc
    17641 caactacagg cgattgcgcc ctaccccagc cagccagtgg tacgcccatc gtggaacgat
    17701 ccggtgcaaa tcaaacaact gctggacgta ggcacacaaa ccttactggt gccgatggta
    17761 caaaacgccg acgaagcccg taaagcggta cgcgccaccc gttatccccc cgccagtatt
    17821 cgcggtgtgg gcagtgcgct ggctcgcgcc tcgcgctgga atcgcattcc tgattacctg
    17881 caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag
    17941 aacttaccgc agattctgga cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat
    18001 ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt
    18061 gagcaggcga tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat
    18121 gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac
    18181 accaccctgc tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtaa tagttcttct
    18241 atttcgatgg cgaagcaaaa caacaactct gtctactaaa cgtacgatgt ggagcgcatc
    18301 atgacgacct ctaccctgca atataaagat aataaaactg ttgaaattga acatcgtgtc
    18361 attaataaat tatttcgtcg cctaatcatt tttcttttta ttctgtttgt tttttcgttt
    18421 cttgatcgca tcaatatagg atttgccgga ctgacaatgg gcaaggatct tggcctgacg
    18481 tccacaatgt ttggcctcgc cgcaacactc ttttatgtca cgtacgtcat gtgcgggatc
    18541 cccagtaata tgatgttagc gaaaattggc gctcgtcgat ggatcgctgg gatcatggtg
    18601 gtctggggaa ttgcttccac ctgcacaatg tttgcaacaa gccccgaaac actctatcta
    18661 ctgcgtatgc tggttggtat tgctgaggcg ggttttttac ctggtatttt ggtttatctg
    18721 acctggtggt tcccggcata tcatcgggcc agagccaatg ccttatttat gatagcgatg
    18781 ccagtcacta tgatgctggg attgatcctt tcaggctaca ttctggcctt ggatggactg
    18841 tggaatctaa aaggctggca atggctcttt ttgctggaag gtctgccatc ggtcattctt
    18901 ggtgtcgtca cctggtttta tctcaacgac acaccagacc aggcgacctg gcttgatgaa
    18961 aacgaaaaac aggctttaaa aacgatgatt ctccgtgaac aagagagtgc atttgcccat
    19021 gcatctgttc cacgctcaac cttacgtgaa gtcctgaccc cagccatcat gctatataca
    19081 cttgcttatt tttgcctgac taatactctg agtgcgatta atatctggac gccgcaaatc
    19141 ctacagagtt tcaatactgg cagtagcaac atcatgattg gtttgctggc ggccatcccc
    19201 caattctgca caatttttgg catgatctgg tggagtcgtc gttccgaccg tcttaaagaa
    19261 cgcaaaaagc atacgattct tccctacctg tttgccgctg ccggatggtt actcgcttct
    19321 gcgacagatc acagcctcat tcaactgagc agcatcatta tggcctcaat gggatcattt
    19381 accgcgatga ctatcttttg gacaacaccg gatcaagtta taagcttgca atcgcgtgcc
    19441 gttgcgatgg ctgtcatcaa tgctgtcggc aatgtaggtt cagcagtcag tccattactt
    19501 atcggtattt tgaaagatgc aaccggcagt tttagctcag gtttgtggtt tgtagcagga
    19561 ttgctggtac tgggcgcgtt agtcatgacc cgaatcccta tggggagtcg ggaacactct
    19621 ctttcatctg aacttatcgc accaaaaccg caataaggag tcatgatgtc cagtgaagct
    19681 atctttgaac atggttatga aaatattgat ttggtccggg aatatgatgc ccgctacgct
    19741 ggtgaagatg tgcattatga aacatttgcc cggttagcga atttctttgg acgcgatatg
    19801 cgccctcact ggcacgatag atattttcaa ctccattatc tggtcactgg acgcattact
    19861 ttacaactgg atgagcactt ctattctgtg cgcgcaccgc tttttgtact aacaccacca
    19921 tcggtgccac atactttctt tactgatcct gatagcgatg ggcatgttct gaccgtacgt
    19981 caggagttaa tttggcccct ggtagaaaag ctctggcctg gtagtggaga tgccgcaatg
    20041 ctacaaggta tctgtctgtc actggaggga atggaacata cacaggcggc attaaatcat
    20101 tattggccga taatatccct ggagtttcaa caaaataagc aaggacggga aattctattg
    20161 caatcactgg cgcaaagtat ttttacaatc ctactgcgtg aagcaccacc aaatgatatt
    20221 tcaacatgca gcgtgcgcgg cgagatgtgt ttattccaga agtttaatcg gctgattgac
    20281 gaaaaatatc gccaacattt aatggtccct gaatatgcaa caatgctcgg tatgagtgaa
    20341 tcacggctta ccgagctgtg ccgacgtttt gccaatcaat cgccgaagag attgattttc
    20401 gaacgagtga gccgggaagc cagacggctt ctgctctata gcgagcaaag catcaaccag
    20461 atagcccttg atcttggcta caaagatccc gcctatttcg cccgtttttt caaccgaatg
    20521 atgggttgcc caccaacaca attccgtggt cgctgaccat ttatcaacct gagagactcg
    20581 acatgaaaat atctttacgc tcactatttg tggcgtgcct gattgcgcca gtggcattca
    20641 cctacgcctc cgaaccgcaa taaatgaaaa ttgtactact tggaacgaaa ggtggccctt
    20701 ctttattaaa agctgaccgt ttaccgatgg caacggcact tattattggc aataaaacct
    20761 agttaattga tgctggttac ggggccagct tacaactggt aaaaaataat attccactgc
    20821 gccagattga taatatttta ctcacccatt tacacagtga tcatattctg gactatccat
    20881 cagtcttaat gaatgcatgg gctagcggtc tgcaggacaa aactattcat gtattcggtc
    20941 cgccaggcac ccgagccatg accgctgcca gttggcaggt cttcgatcgg gacatcacac
    21001 tgcgtatgga agaagaagga aaacccgatc ctcgcaaact cgttacagct caagatgttg
    21061 gtgaaggcgt agtttataaa gatgataccg tgaccgtgac tgcattaaag gtccctcatt
    21121 ctcctttccc tgacggtgag gcttttgctt atcgcttcga cacacaaggc aagcgaattg
    21181 tcttctcagg agatacctcg tactttcccc cactggcaac tttctcagaa ggcgctgata
    21241 tacttgtgca tgaggccgta catgagccct ccgtcgctaa gctggcagac agtattggca
    21301 atggtaaaac actggcaaaa gccattgctt ctcaccatac gactattgag gatgtaggca
    21361 aaattgccaa tgaagcaaaa gtgaaaaaac tcatccttag ccacctggta ccaatcaccg
    21421 ttccggatga aacatggaag caagaagcac agaaaactat tcaggcccta tcgtcgttgg
    21481 tcatgaaaat atgacgatta ccattccatt cgccccataa aaaattccgt ccccgttagc
    21541 ccttgttagc ggggaatatt atgatttgcc ttactgtaag ttgatcacga tcgcagttta
    21601 ctatttaact ccaccttgct gtcgaaaagt accaacgttc gcctgaaacg tctcttcccg
    21661 acaaccgcgt tcaacgcttc aattaaataa caaaaacaac acacaaaatt aacaatcgta
    21721 ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac
    21781 gtcctttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct
    21841 atggcgagcg agtgaaagac gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg
    21901 ttgcccaact gtacgacgcg ctgcacaaac cggagatgca ggactctctg tgttggaaca
    21961 ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg
    22021 acctgcgcca gcaacgcgac gccatcgctg aatggtcacg cctgagctat ggctggatgg
    22081 gccgtacccc agactacaaa gccgctttcg gttgcgcact gggcgcgaat ccgggctttt
    22141 acggtcagtt cgagcagaac gcccgtaact ggtatacccg tattcaggaa actggcctct
    22201 actttaacca cgcgattgtt aacccaccga tcgtcatttg ccgaccgata aagtgaaaga
    22261 cgtttacatc aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt
    22321 ggttgccacc aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt
    22381 gatgggcgaa aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt
    22441 gaaattaatc tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga
    22501 ctacccgctc tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtact
    22561 gatcccatgg gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc gctggacgat
    22621 ggaaggcggt ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact
    22681 cgacttcatt acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg
    22741 tggtgtgcag gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag
    22801 tgactcgatg tgttcagaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc
    22861 cgcactgcaa acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat
    22921 agaacgcaac gttaccagtg gcctgatcta cctcccttcc agtgcccgtg acctgaacaa
    22981 tccgcagatc gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt
    23041 ccagcgcatc aagatcctca aactgatgtg ggacgccatt ggcagcgaat ttggtggtcg
    23101 tcacgagctg tatgaaatca actactccgg tagccaggat gagattcgcc tgcaatgtct
    23161 gcgccaggca caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct
    23221 gtcggaatac gaccagaacg gctggactgt gccgcacctg cacaacaacg acgatatcaa
    23281 catgctggat aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa
    23341 cgcctgcgct ttcatgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc
    23401 gagggcgacg ccggacaatg cgggattacg gcaacggctg tctgctcggt cacggataca
    23461 ccaccgtcgc tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc
    23521 aacggcaagt tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc gcgccacttc
    23581 gcgggcatga caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt
    23641 ccgctggcgc agccggtgct aaaaggttcg ctggccagtc ttgaaggaga gatccgcgat
    23701 gtgcaggcga ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt
    23761 gcagaaggtc acggacttat ttactttaaa cgccgtttcc atccggtgat gctggaaatg
    23821 gaagctgcga tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg
    23881 atctcaatca catatctacc cgatgaataa ttcttgccca accgctcacc gccattcctg
    23941 accgcttaca tccctaaaat aaccactcag ttatttacct tactttacgc gcacgtatct
    24001 ctggcaacat cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag
    24061 aacggcttcg gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc
    24121 aagtgagtga atccccggga gcttacaaaa gtaagtgtca gaggtgaacg aacgcagctg
    24181 ccgcacctgt aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa
    24241 aactattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
    24301 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt
    24361 cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac
    24421 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
    24481 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg
    24541 tgggaccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg
    24601 gggtagtgct ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta
    24661 acggcgcatc tctcggtgag atgatcaaag aagagatggg gccagtaccg gggactatcg
    24721 cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg
    24781 ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg
    24841 cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg
    24901 ttattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
    24961 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
    25021 atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg
    25081 caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc
    25141 cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag
    25201 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
    25261 cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca
    25321 ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt
    25381 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatca
    25441 ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga
    25501 aagacgttac cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag
    25561 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
    25621 gtgcgccgac gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg
    25681 acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc
    25741 tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
    25801 cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg
    25861 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
    25921 tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca
    25981 ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg
    26041 tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc
    26101 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
    26161 acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
    26221 caggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
    26281 cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa
    26341 tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc
    26401 ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat
    26461 ctcggccagg cggcgaagat gttgattggt attccagact acgacaacta cgtcgagcat
    26521 atgaagacca accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc
    26581 cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag
    26641 catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca aaactaccct
    26701 gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt
    26761 cggcgaagtc tccgttgatg atcaactgat tggcgaccgc gccacgcaga tcaaaacgct
    26821 gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaggacgcac tactcgacct
    26881 gctggataat ctcgacaagg gcaatattca gttcgatcgt ctggtgattg aatgcaccgg
    26941 catggccgat cccggcccga ttattcagac ctttttctcc catgagattt tatgccagct
    27001 ctacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa
    27061 ccagttcacc atcgcccagt cccaggtggg ctacgccgac cgtattctgc tgactaaaac
    27121 cgacgtcgca ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc
    27181 accggtctac accgtcaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg
    27241 ttttatgctg gaagagaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa
    27301 acaaaacgat atttcgtcaa ttgtggtgga actggattac ccggtagata tcagcgaagt
    27361 ttcccgcgtg atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg
    27421 gatgctgtgg attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta
    27481 cagcgccgac tgggacaggc cgtggggcga tgaaaaaccg catagcacaa tggtgtttat
    27541 cgggattcag ttgccggagg aagagattcg ggcggcgttt gcgggattga ggaagtaagt
    27601 tcgggaaaga tcaacttttt gagagttcca ccgcaacatt attacgagtg gaactcttta
    27661 gatatgtcag accaaccaaa gcttcttcaa ttgcgttttc attttttcat gtctcatttt
    27721 cagttcagcc aatccccatt ctgaagaatt acaaacatct tttgttaaat aaaaagagct
    27781 tttactatta agtttctcat aaatagattt ctttttgata aaatcatagt gttgtgtttc
    27841 agaatttttg gaaccactta tgagtgttaa attacctatt ttatgcagcc agtagacatg
    27901 ctcttgaggt tggaatctat taatccagta ttcattaact aatgcttgag gaagaatatg
    27961 ctcaattgtg attctccccg tataggtttt aatcacgctc tcatcttgtt gctccatatc
    28021 taagcgaagc aaaattgctt ttattaagtt cacttgattc ggacgaggct catataaatc
    28081 ttcatccaga gcagctataa aacctgaatt atctgcatgt tggtttatct gatttatcac
    28141 agaatcaaaa ggcatatcat tatttattgc taccagagca gaataacaaa ccatttctct
    28201 ttggctttta atttgttttt ttaaccaccc atgcatatat accttctcaa aggcggtaat
    28261 aaattgagaa aaatcatcta aattaaaatc ttctgtacga gccattcgat tcatgaatgc
    28321 cataaccgga ggaatccact catctacccc taaattactt agcgatgcta tttttcgtcg
    28381 tattgaaggg tgttcaaaat cattctctaa aatcttagta taattt
//
LOCUS       DAEEDS010000050        28406 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEEDS010000050 DAEEDS010000000
VERSION     DAEEDS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28406
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.050"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KLH32_002382"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KLH32_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBE0139178.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="KLH32_002383"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="KLH32_002383"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBE0139179.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="KLH32_002384"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="KLH32_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBE0139180.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="KLH32_002385"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="KLH32_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBE0139181.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="KLH32_002386"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="KLH32_002386"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE0139182.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQIAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="KLH32_002387"
     CDS             6428..7057
                     /locus_tag="KLH32_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBE0139183.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALFGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KLH32_002388"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KLH32_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBE0139184.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8732..10075)
                     /gene="melA"
                     /locus_tag="KLH32_002389"
     CDS             complement(8732..10075)
                     /gene="melA"
                     /locus_tag="KLH32_002389"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBE0139185.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="KLH32_002390"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="KLH32_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBE0139186.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="KLH32_002391"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="KLH32_002391"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBE0139187.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="KLH32_002392"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="KLH32_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBE0139188.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="KLH32_002393"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="KLH32_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBE0139189.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="KLH32_002394"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="KLH32_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBE0139190.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="KLH32_002395"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="KLH32_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBE0139191.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="KLH32_002396"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="KLH32_002396"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBE0139192.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KLH32_002397"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KLH32_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBE0139193.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KLH32_002398"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KLH32_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBE0139194.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="KLH32_002399"
     CDS             complement(21748..22626)
                     /locus_tag="KLH32_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBE0139195.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KLH32_002400"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KLH32_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBE0139196.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25253..25588
                     /locus_tag="KLH32_002401"
     CDS             25253..25588
                     /locus_tag="KLH32_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBE0139197.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25746..26189
                     /gene="yjdN"
                     /locus_tag="KLH32_002402"
     CDS             25746..26189
                     /gene="yjdN"
                     /locus_tag="KLH32_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBE0139198.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26322..27110
                     /gene="phnC"
                     /locus_tag="KLH32_002403"
     CDS             26322..27110
                     /gene="phnC"
                     /locus_tag="KLH32_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBE0139199.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27135..28151
                     /gene="phnD"
                     /locus_tag="KLH32_002404"
     CDS             27135..28151
                     /gene="phnD"
                     /locus_tag="KLH32_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0139200.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28257..>28406
                     /locus_tag="KLH32_002405"
     CDS             28257..>28406
                     /locus_tag="KLH32_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBE0139201.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT         7297 a         7422 c         6934 g         6753 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagcactga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacagattgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctgacagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgtttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca actagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga cgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgcaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct ggatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccgcgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gtatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gtcgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gcgctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg caactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcattagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttacg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
    25261 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
    25321 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
    25381 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
    25441 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
    25501 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
    25561 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
    25621 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
    25681 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
    25741 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
    25801 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
    25861 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
    25921 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
    25981 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
    26041 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
    26101 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
    26161 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
    26221 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
    26281 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
    26341 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
    26401 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
    26461 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
    26521 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
    26581 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
    26641 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
    26701 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
    26761 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
    26821 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
    26881 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
    26941 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
    27001 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
    27061 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
    27121 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
    27181 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
    27241 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
    27301 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
    27361 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
    27421 tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
    27481 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
    27541 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
    27601 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
    27661 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
    27721 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
    27781 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
    27841 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
    27901 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
    27961 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
    28021 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
    28081 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
    28141 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
    28201 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
    28261 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
    28321 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
    28381 aagacggcgg caacatggca accttc
//
LOCUS       DAEEDS010000051        28338 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEEDS010000051 DAEEDS010000000
VERSION     DAEEDS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.051"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KLH32_002406"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KLH32_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="KLH32_002407"
     CDS             complement(998..2020)
                     /locus_tag="KLH32_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0139202.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="KLH32_002408"
     CDS             complement(2034..3536)
                     /locus_tag="KLH32_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE0139203.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KLH32_002409"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KLH32_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="KLH32_002410"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="KLH32_002410"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBE0139204.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="KLH32_002411"
     CDS             complement(5851..6192)
                     /locus_tag="KLH32_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBE0139205.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KLH32_002412"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KLH32_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBE0139206.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KLH32_002413"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KLH32_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBE0139207.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="KLH32_002414"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="KLH32_002414"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBE0139208.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="KLH32_002415"
     CDS             12871..14214
                     /locus_tag="KLH32_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE0139209.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="KLH32_002416"
     CDS             complement(14276..14482)
                     /locus_tag="KLH32_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBE0139210.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="KLH32_002417"
     CDS             14807..15361
                     /locus_tag="KLH32_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBE0139211.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="KLH32_002418"
     CDS             complement(15424..16362)
                     /locus_tag="KLH32_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139212.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KLH32_002419"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KLH32_002419"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBE0139213.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="KLH32_002420"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="KLH32_002420"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBE0139214.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="KLH32_002421"
     CDS             complement(19573..19953)
                     /locus_tag="KLH32_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE0139215.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="KLH32_002422"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="KLH32_002422"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE0139216.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="KLH32_002423"
     CDS             21011..21976
                     /locus_tag="KLH32_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0139217.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="KLH32_002424"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="KLH32_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBE0139218.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KLH32_002425"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KLH32_002425"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBE0139219.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KLH32_002426"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KLH32_002426"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0139220.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="KLH32_002427"
     CDS             25350..25895
                     /locus_tag="KLH32_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBE0139221.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="KLH32_002428"
     CDS             complement(25879..26526)
                     /locus_tag="KLH32_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0139222.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="KLH32_002429"
     CDS             26738..27529
                     /locus_tag="KLH32_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBE0139223.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="KLH32_002430"
     CDS             27539..>28338
                     /locus_tag="KLH32_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBE0139224.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7542 c         6989 g         6854 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DAEEDS010000052        27590 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEEDS010000052 DAEEDS010000000
VERSION     DAEEDS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27590
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.052"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="KLH32_002431"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="KLH32_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBE0139225.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="KLH32_002432"
     CDS             complement(1614..1937)
                     /locus_tag="KLH32_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBE0139226.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KLH32_002433"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KLH32_002433"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBE0139227.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KLH32_002434"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KLH32_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBE0139228.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSYARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KLH32_002435"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KLH32_002435"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBE0139229.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KLH32_002436"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KLH32_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBE0139230.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="KLH32_002437"
     CDS             7537..8574
                     /locus_tag="KLH32_002437"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE0139231.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KLH32_002438"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KLH32_002438"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBE0139232.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="KLH32_002439"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="KLH32_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBE0139233.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KLH32_002440"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KLH32_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBE0139234.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11615
                     /locus_tag="KLH32_002441"
                     /pseudo
     CDS             10745..11615
                     /locus_tag="KLH32_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(11650..12984)
                     /gene="srmB"
                     /locus_tag="KLH32_002442"
     CDS             complement(11650..12984)
                     /gene="srmB"
                     /locus_tag="KLH32_002442"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBE0139235.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13116..13853
                     /gene="trmN"
                     /locus_tag="KLH32_002443"
     CDS             13116..13853
                     /gene="trmN"
                     /locus_tag="KLH32_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBE0139236.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13838..15460)
                     /gene="nadB"
                     /locus_tag="KLH32_002444"
                     /pseudo
     CDS             complement(13838..15460)
                     /gene="nadB"
                     /locus_tag="KLH32_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15716..15871
                     /gene="rseD"
                     /locus_tag="KLH32_002445"
     CDS             15716..15871
                     /gene="rseD"
                     /locus_tag="KLH32_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBE0139237.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15868..16443
                     /gene="rpoE"
                     /locus_tag="KLH32_002446"
     CDS             15868..16443
                     /gene="rpoE"
                     /locus_tag="KLH32_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBE0139238.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16476..17126
                     /gene="rseA"
                     /locus_tag="KLH32_002447"
     CDS             16476..17126
                     /gene="rseA"
                     /locus_tag="KLH32_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBE0139239.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17126..18082
                     /gene="rseB"
                     /locus_tag="KLH32_002448"
     CDS             17126..18082
                     /gene="rseB"
                     /locus_tag="KLH32_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBE0139240.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18079..18558
                     /gene="rseC"
                     /locus_tag="KLH32_002449"
     CDS             18079..18558
                     /gene="rseC"
                     /locus_tag="KLH32_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBE0139241.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18756..20555
                     /gene="lepA"
                     /locus_tag="KLH32_002450"
     CDS             18756..20555
                     /gene="lepA"
                     /locus_tag="KLH32_002450"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBE0139242.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20571..21545
                     /gene="lepB"
                     /locus_tag="KLH32_002451"
     CDS             20571..21545
                     /gene="lepB"
                     /locus_tag="KLH32_002451"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBE0139243.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21817..22497
                     /gene="rnc"
                     /locus_tag="KLH32_002452"
     CDS             21817..22497
                     /gene="rnc"
                     /locus_tag="KLH32_002452"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBE0139244.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22494..23399
                     /gene="era"
                     /locus_tag="KLH32_002453"
     CDS             22494..23399
                     /gene="era"
                     /locus_tag="KLH32_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBE0139245.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23411..24139
                     /gene="recO"
                     /locus_tag="KLH32_002454"
     CDS             23411..24139
                     /gene="recO"
                     /locus_tag="KLH32_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBE0139246.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24151..24882
                     /gene="pdxJ"
                     /locus_tag="KLH32_002455"
     CDS             24151..24882
                     /gene="pdxJ"
                     /locus_tag="KLH32_002455"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBE0139247.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24882..25262
                     /locus_tag="KLH32_002456"
     CDS             24882..25262
                     /locus_tag="KLH32_002456"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBE0139248.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25682..25762
                     /gene="shoB"
                     /locus_tag="KLH32_002457"
     CDS             25682..25762
                     /gene="shoB"
                     /locus_tag="KLH32_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBE0139249.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25956..26216)
                     /gene="yfhL"
                     /locus_tag="KLH32_002458"
     CDS             complement(25956..26216)
                     /gene="yfhL"
                     /locus_tag="KLH32_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBE0139250.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26431..27527
                     /locus_tag="KLH32_002459"
                     /pseudo
     CDS             26431..27527
                     /locus_tag="KLH32_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6805 a         6865 c         7230 g         6690 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtagctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgttgctgcc gcacatttat gaacttaccc gagtgatgga tacgcttcgt gaggctgcca
    10981 aaaaggagtc agatccggac ggggaactac gcgttgtttc gggcgaaacg ttgctctctt
    11041 accgaatgcc gcaggtgttg cagcgttttc ggcaacgcgc accaaaagtg cgtttatcgt
    11101 tgcaggcgct gaactgttac gtgatccgtg atgcgctact gaatgatgag gccgatgtcg
    11161 gcgtctttta tcgcgtaggg aatgatgatg ccctgaaccg acgagagttg ggtgaacaat
    11221 cgctggtgct ggtggcttca ccacaaattg cagatgtcga ttttactgag ccgggaagac
    11281 ataacgcctg tagctttatt atcaacgaac cgcaatgtgt cttccggcag atatttgaga
    11341 gcacgctgcg acagcggcgg atcacggtgg aaaacaccat tgagcttata agtatcgaaa
    11401 gcatcaagcg ttgtgtcgcg gcgaatatcg gtgtcagtta cttgccccac tttgcggtgg
    11461 aaaaagagat tgagagcgga gaattaattg aattgccttt tggcgaacag tcgcagacca
    11521 ttacggcaat gtgcgcacat tatgctggaa aagcggttag cccggcgatg cacattttta
    11581 ttcagtgtgt tgaagagtgt tttgtggttg cgtaaaaaaa caatgccggg cttaaacccg
    11641 gcattgagat tactcttctg tcgtttgcgg tggcacgccc gttccgctcg gtttacggcg
    11701 cttaccaata tttttggtgt cgcgatggcg ttttttcacc cgcggcttct ctttctcttt
    11761 agcttttttc ttctcggcgc gttttgccag tactttcttc gatggcttgc cggtctgctt
    11821 ctcgcttggc gcacgcgttt tcgggcgtaa ctcatcaata acgcgagctt taatcggctc
    11881 ttcaatgtaa cggccaacct tgcccagcag caaatggtca tgggcttcca ccagcgaaat
    11941 tgcggtgcct ttacgaccgg cgcgcgcggt acgtccgata cgatgcaaat aagtatcgcc
    12001 actgcgcggc atatcgaagt taaagacgtg gctgacgtca ggaatgtcga taccgcgcgc
    12061 ggcaacatcg gtggcaacca gtacgtttac gcggccttcg gtcaggcgtt taattgcttc
    12121 gttacgcttg ccttgtacca tctcaccttc gagatagcag ttgttgatgc ccgcttcgcg
    12181 cagccagttt gccagctcat gcacacgttc acgcttacgc acaaacacga ttgagcgagt
    12241 cgcttcaggc tgttttaaca ggtgcaccag caatgcggtt ttatgttcca ggtcatcggc
    12301 gcggtaatac cactgatgaa tttttttgcg ctcacgggtg gacggattgg cagaaacttc
    12361 caccggatct tccagcagac gctcggcaaa gtcctgaatc gcatcgcctt ccagcgttgc
    12421 cgaaaagagc agggtctgtt tacgccagcg agtttcgcca gcaatatgtt cgatatcctg
    12481 agcgaagccc atatccagca tacggtctgc ttcgtcgagg atcagcgttt caaccgcgcg
    12541 gcaatcgaag ttctcttctt ttatgtattg cagcagacgt ccggtcgtgg cgaccacgat
    12601 gtcctgattt tcgctgaaca cttccgcgtg gttcatatag gctacgccgc cggtgatggt
    12661 ggcgatatcc agatgcgtat gtttcgccag ttcgcgggca tgatcggcca cctgcatcgc
    12721 cagctcgcga gttggggtga ggatcaaaat acgcggcgga ccggatttct tacgtgggaa
    12781 atcgagcagg tgctgcaacg ctggcagcag atacgccgcc gttttaccgg tgcctgtcgg
    12841 cgcagaaccg agtacatcac ggccatcgag cgcaggcgga atggcggcag cctgaatggc
    12901 ggtcgggcga gtgaaacctt tatcctggag ggcttccagc aggctttcgt cgagttcaag
    12961 ttcggaaaaa gtcgttacag tcatgttcta cctctgtgtg gggcgctgat tatagacgtt
    13021 acggctgcaa tcttcatctg tttgtatgga tatcgcttct cgggtattgt tctcacccgg
    13081 tgctatcgcc gtttttcctg caaggtttta ttttcatgtc acagtctaca tccgtgcttc
    13141 gtcgtaatgg atttactttt aaacagtttt ttgttgctca cgatcgctgt gcgatgaaag
    13201 tgggaacgga tggcattttg ctgggcgcat gggcaccggt ggctggggtg aaacgttgcc
    13261 ttgatatcgg cgcgggtagc gggttgctgg cactgatgct ggcgcagcga accagtgaca
    13321 gcgtgataat tgatgccgtt gagctggaaa gtgaagctgc cgcgcaggcg caggaaaata
    13381 tcaaccagtc cccgtgggca gagcggatta acgtccatac ggcggatatt cagcagtgga
    13441 tcacacagca gacagtacgc ttcgatttaa tcatcagtaa cccaccttac tatcagcagg
    13501 gagtagagtg cgcgacacct caacgggaac aggctcgcta taccactacg cttgatcacc
    13561 catcgctgct gacctgtgcg gcggagtgca ttaccgaaga gggatttttc tgcgtggtgc
    13621 tgccagagca gattggtaat ggttttactg aactggcatt aagcatgggc tggcatttac
    13681 gtttgcggac ggatgtggcg gaaaacgaag cgcgactgcc gcatcgggtg ctgctggcat
    13741 tctccccgca ggcgggagaa tgctttagcg atcgcttagt cattcgtggg ccagaccaga
    13801 actattccga agcgtatacg gcgctgaccc aggcttttta tctgtttatg taatgattgc
    13861 cgggggaaag gatcgatgga ccggaatgcg tgagcagttc cgggtaatcc agcgtaaaat
    13921 gcagcccccg gctttctttg cgcatcatcg cgcagcgaac aatcaactcg gcaacctgta
    13981 ccagattacg cagctccagc aaattatttg agacgcggaa atgggcgtaa tattcgtcta
    14041 tttcttgttg gagcatggtt atccgccgca gggcgcgttc caggcgcttc gttgtgcgca
    14101 caatgccaac gtaatcccac ataaacagcc gtagctcgtg ccagttatgc tgaattacta
    14161 cccgttcgtc agggttctca acgcggcttt catcccacgg cggtaacgta ctgacgccgt
    14221 gggcataagg catacgtctg gtgatatctt ctgccgccga ccagccatag accagacact
    14281 ccagcaatga attcgaggcc atgcggttag cgccgtgtaa gccggtataa ctcacctcgc
    14341 caatggcata caagccctcg acgtccgtac gcccatgatc atcaaccatt acaccacctc
    14401 aggtatagtg tgcagcaggc acaatcggta ccggttcttg tgtgagatca atccccagcc
    14461 cgagcaattt ttcataaatc atcgggaaat gctggcgaat aaaatcggcg ggcttatggc
    14521 tgatgtcgag gaacatacaa tctgcgccga ggcgtttcat ttcatggtca atggcgcggg
    14581 cgacaatatc gcgcggggcc agttcgccgc gctcatcaaa atcgggcata aaacgcgtgc
    14641 catccgggcg cttgagataa gcgccttcgc cgcgcagtgc ttccgttaac aggaaattgc
    14701 gtgcctgtgg gtgatacagt gcggtagggt ggaactgatt aaattcgaga ttggcaaccc
    14761 gacagcccgc gcgccacgcc atagcaatgc catcgccaga agaaatatcc ggattggtgg
    14821 tgtactgata caccttcgac gcaccgccgg ttgccagtac cactgctttt gcgtggcagg
    14881 tttccaccgt ttctttattt cggttccata cccacgcgcc tacaacccgt cgcgtgcccg
    14941 gcaggccaat tttgtcagag acaatcagat caactgcgtt gctgcgctcc agcacacgaa
    15001 tattcggatg gttctgcgcc ttgctcacca gcgtggattg tacttctcta ccggtggcgt
    15061 cggcggcatg aagaatacga cggtgactat gtccaccttc acgggtcaga tggtagcttt
    15121 cttcgccatt cggttgaacg tgggtatcaa acaaaacccc ctggtcaatt aaccatggca
    15181 cacaggaacg agcattgctg gcgacaaatt caactgcatg gtgatcgcaa ataccagccc
    15241 cggcaatcaa tgtgtcttcc acatgcgagt caatgctgtc agtttcatca aacacggcgg
    15301 caataccgcc ctgggcataa aatgttgaac cttccgttac cgggccttta cttagaacga
    15361 tgacctgatg ctggtcagcc aggcgtagcg ccagtgaaag tccggctgcg ccgctaccaa
    15421 taatcaacac gtcacatgaa tgttcaggga gagtattcat tttctttgtt taatttacta
    15481 aacattgttt ggtcagcata gcatcatgtt gtgcgaataa acacctgcta ttttaatatt
    15541 tgttacagtg gctaaacacg ttgacgcagg gcggcgagaa aaagagaagt tactggctgg
    15601 tggaggatta ggtggtgaaa taaaaaggcc gttgggttac tcttcaggca gttaaatggg
    15661 catttctaca cagataatgc gatgttcaga ttctgtagac ttataatgat agataatgat
    15721 ccgtctacag catgacaaac aaaaacagat gcgttacgga actttacaaa aacgagacac
    15781 tctaaccctt tgcttgctca aattgcagct aatggagtgg cgtttcgata gcgcgtggaa
    15841 atttggtttg gggagacttt acctcggatg agcgagcagt taacggacca ggtcctggtt
    15901 gaacgggtcc agaagggaga tcagaaagcc tttaacttac tggtagtgcg ctatcagcat
    15961 aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg atgttcccga tgtggtacaa
    16021 gaagctttta ttaaagccta tcgtgcgctg gattcgttcc ggggagatag cgctttttat
    16081 acatggctgt atcggattgc tgtaaataca gcgaaaaatt acctggttgc tcaggggcgt
    16141 cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa acttcgaaag tggcggcgcg
    16201 ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag aagaactgag acagatagtt
    16261 ttccgaacta ttgagtccct cccggaagat ttgcgcatgg caataacctt gcgggagctg
    16321 gatggcctga gctatgaaga gatagccgct atcatggatt gtccggtagg tacggtgcgt
    16381 tcacgtatct tccgagcgag ggaagctatt gataacaaag ttcaaccgct tatcaggcgt
    16441 tgacgatagc gggatactgg ataagggtat taggcatgca gaaagaacaa ctttccgctt
    16501 taatggatgg cgaaacgctg gatagtgagc tgcttaacga actggctcat aacccagaaa
    16561 tgcagaaaac ctgggaaagc tatcacttaa tccgtgattc aatgcggggt gatactcccg
    16621 aggtgctcca tttcgatatc tcttcacgcg tgatggccgc cattgaagaa gagccagtac
    16681 gtcaatcggc gacattgatc ccggaagccc agcctgcgcc gcatcaatgg cagaaaatgc
    16741 cattctggca gaaagtacgt ccgtgggcgg cacagcttac ccaaatgggc gtagccgcat
    16801 gcgtatcgct tgcagttatc gttggcgtcc agcactataa tggacaatct gaaacgtccc
    16861 agcagcccga aacgccggta tttaatacac tgccgatgat gggtaaagcc agcccggtaa
    16921 gcctgggagt accttctgaa gcgaccgcaa acaatggtca acagcagcag gtacaggagc
    16981 agcgtcgtcg cattaatgca atgttgcagg attacgaact gcaacgccga ctccactctg
    17041 aacagcttca gtttgagcag gcgcaaaccc agcaagccgc tgtacaggtg ccaggaattc
    17101 aaactttagg aacgcaatcg cagtaatgaa gcaactttgg tttgccatgt cattagtgac
    17161 aggtagcctg ttattctctg ctaacgcctc ggccactccc gcgtccgggg cgttattaca
    17221 gcagatgaac ctggccagtc agtcactgaa ttacgagctg tcattcatca gcatcaataa
    17281 acagggtgtt gagtctctgc gttatcgaca tgcacgcctc gataaccgtc ctcttgcaca
    17341 attgttgcaa atggatggcc cgcgccggga agtggtacag cgcggcaatg aaatcagcta
    17401 ttttgaaccg ggacttgaac cgttcacgct taatggcgat tacattgttg attctctgcc
    17461 atcgcttatc tataccgatt tcaaacgcct ttctccttac tacgacttta tctccgtcgg
    17521 gcgcacgcgt attgctgatc gtctttgcga agtcattcgc gtggttgccc gagatggtac
    17581 acgctacagc tacatcgtgt ggatggacac cgaatcgaaa ttaccgatgc gggttgatct
    17641 ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg attgctttta acgtcaatca
    17701 ggatatcagc agcagtatgc agacgctggc gaaggcaaat ttgccgccgt tgctttctgt
    17761 tcctgtaggt gaaaaagcta aattcagctg gacgccaacc tggttgccac agggttttag
    17821 cgaagtttcc agtagtcgac gtccgctacc gacgatggac aacatgccta tcgaatcacg
    17881 tctctattcc gacggattat tcagcttctc ggtaaacgtt aaccgcgcta cgccatcgag
    17941 caccgatcag atgttgcgca ccggacgcag aaccgtcagt acaagcgtac gtgataacgc
    18001 cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg aaacgcattg ccgagaatat
    18061 taagttcggg gcagcgcaat gatcaaagag tgggctaccg tcgtctcctg gcaaaacggg
    18121 caggcgctgg tcagttgtga tgttaaagct tcatgcagca gctgcgcttc acgcgccggt
    18181 tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca cgcataccat tgtcgtaccc
    18241 tgtgatgagc cgttagtgcc ggggcaaaaa gtggaattgg ggatcgccga aggcagcctg
    18301 cttagctccg cattactggt ttatatgtcg ccgctggtgg gattattcct catcgcttcg
    18361 ctatttcagc tactctttgc ttctgacgtc gcagcattat gtggtgcggt cctcggcggt
    18421 attggcgggt tcctgattgc gcgcggctac tcgcgtaagt ttgctgcccg ggcagaatgg
    18481 caaccgatca tcttaagcgt ggcattgccg cctggccttg tgcgatttga aacatcttcg
    18541 gaagacgcga gccagtgatt tcactggctt tgttgcattt tgcataacaa atgcgaggac
    18601 gtttccagat accacaatcc atacggtatg aacatttcct cgcctcaatg ttgtagtgta
    18661 gaatgcggcg tttctattaa tacagacgtt aagctcagaa cagcgacttc taaaagcctg
    18721 gttaaccggg cattaaggca caataatcat actttatgaa gaatatacgt aacttttcga
    18781 tcatagctca cattgaccac ggtaaatcga cgctgtctga ccgtattatc cagatctgcg
    18841 gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga ttccatggat cttgagcgtg
    18901 agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga ctacaaagcg tctgacggcg
    18961 aaacctatca gcttaacttt atcgacaccc cgggccacgt agacttctcc tatgaagttt
    19021 cccgttcgct ggctgcctgt gaaggtgcat tgctggtggt cgacgccggg cagggcgtag
    19081 aagcgcaaac cctggcaaac tgctacaccg ccatggaaat ggatctcgaa gtggtgccgg
    19141 tactgaacaa gattgacctg ccggcagccg atcctgaacg cgtggcggaa gaaattgaag
    19201 atatcgtcgg catcgacgcc accgatgcag tgcgctgttc agcgaaaacc ggcgttggcg
    19261 tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc gccggaaggc gatccggaag
    19321 gcccgttgca ggcactaatt atcgactcct ggttcgacaa ctacctgggc gttgtttcac
    19381 ttatccgtat taaaaacggc accctgcgta agggcgacaa agtgaaagtc atgagtaccg
    19441 ggcagaccta taacgccgac cgtctgggca tcttcacgcc gaaacaggtt gaccgcactg
    19501 aactgaaatg tggtgaagta ggctggctcg tatgtgcgat taaagatatc cacggcgctc
    19561 cagtcggcga taccttaacg ctggcgcgta atccggcaga aaaggcgctg cctggcttta
    19621 agaaagtcaa accgcaggta tacgccggtc tgttcccggt aagttccgac gactatgaag
    19681 ccttccgtga cgcgctgggt aaactcagcc tgaacgatgc ctcactgttc tatgagccgg
    19741 aaagctccag cgcgctgggc tttggtttcc gctgcggctt ccttggcctg ctgcacatgg
    19801 agatcatcca ggagcgtctg gaacgtgaat acgatctgga tctgatcacc actgcgccga
    19861 ccgtagtgta tgaagttgaa accacgtcaa gggaagttat ctacgtcgac agcccatcca
    19921 agctgcctgc ggtaaataac atttacgaac tgcgcgagcc gattgcagag tgtcacatgc
    19981 tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg cgtagaaaaa cgcggcgtgc
    20041 agaccaatat ggtttaccac ggtaatcagg tggcgctgac gtacgagatc ccgatggcgg
    20101 aagtggtgct cgacttcttc gatcgcctga aatctacctc gcgtggttat gcgtctctgg
    20161 attacaactt caaacgcttc caggcgtccg acatggtacg tgtagacgta ttaatcaacg
    20221 gtgaacgtgt tgatgcgctg gcgttgatca cccaccgtga taattcgcaa aaccgaggtc
    20281 gcgagttggt ggagaagatg aaagatctga tcccacgcca gcagtttgat atcgccattc
    20341 aggcagcgat tggtacgcac attattgcgc gatccaccgt gaaacagctg cgtaaaaacg
    20401 tactggctaa atgttatggc ggcgatatca gccgtaagaa aaagctgctg cagaagcaga
    20461 aagaaggtaa gaaacgcatg aagcagatcg gtaacgttga gctgccgcag gaagcgttcc
    20521 tcgccattct gcacgtcggc aaagacaaca aataaccctt aggagttggc atggcgaata
    20581 tgtttgccct gattctggtg attgccacac tggtgacggg cattttatgg tgcgtggata
    20641 aattcttttt cgcacctaaa cggcgggaac gtcaggcagc ggcgcaggcg gctgccggtg
    20701 actcactgga taaagcaacg ttgaaaaagg ttgcaccgaa gcctggctgg ctggaaaccg
    20761 gagcttctgt ttttccggtg ctggctatcg tattgattgt acgttcgttt atttatgaac
    20821 cgttccagat cccgtcaggt tcgatgatgc cgactctgtt aatcggtgat tttattctgg
    20881 tagagaagtt tgcttatggc attaaagatc ctatctacca gaaaacgctg atcgaaaccg
    20941 gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc ggaagatcca aagcttgatt
    21001 acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac ttacgatccg gtctcaaaag
    21061 agctgacgat tcaaccggga tgcagttccg gccaggcgtg tgaaaacgcg ctgccggtca
    21121 cctactcaaa cgtggaaccg agcgatttcg ttcagacctt ctcacgccgt aatggtgggg
    21181 aagcgaccag cggattcttt gaagtgccga aaaacgaaac caaagaaaat ggaattcgtc
    21241 tttccgagcg taaagagaca ctgggtgatg tgacgcaccg aattctgaca gtgccgattg
    21301 cgcaggacca ggtggggatg tattaccagc agccagggca acaactggca acctggattg
    21361 ttccgccggg acaatacttc atgatgggcg acaaccgcga caacagcgcg gacagccgtt
    21421 actggggctt tgtgcctgaa gcgaatctgg tcggtcgggc cacggctatc tggatgagct
    21481 tcgataagca agaaggcgaa tggccgactg gtgtgcgctt aagtcgcatt ggcggcatcc
    21541 attaatagcc atcttcgttc acgttgtcgc cgttatggcg acaacgtgaa ttatttatga
    21601 gataaatctc ccgtggctaa cgacatcccc cgtcgttgtg tatagaatat tcccccgaag
    21661 tttaaggttg gcacctccag gttgccacgg cacacgaaac agcgttggtc cccatatacc
    21721 gctaaactga aactgcagcg aagcagttag cagagccatg tatatcaggt ctgtttcgtg
    21781 tgctgaattg ttgacgcatt tatttattgg tatcgcatga accccatcgt aattaatcgg
    21841 cttcaacgga agctgggcta cacttttaat catcaggaac tgttgcagca ggcattaact
    21901 catcgtagtg ccagcagcaa acataacgag cgtttagaat ttttaggcga ctctattctg
    21961 agctacgtta tcgccaatgc gctttatcac cgtttccctc gtgtggatga aggcgatatg
    22021 agccggatgc gcgccacgct ggtccgtggc aatacgctgg cggaactggc gcgcgaattt
    22081 gagttaggcg agtgcttacg tttagggcca ggtgaactta aaagcggtgg atttcgtcgt
    22141 gagtcaattc tcgccgacac cgtcgaagca ttaattggtg gcgtattcct cgacagtgat
    22201 attcaaaccg tcgagaaatt aatccttaac tggtatcaaa ctcgtctgga cgaaattagc
    22261 ccaggcgata aacaaaaaga tccgaaaacg cgcttgcaag aatatttgca gggtcgccat
    22321 ctgccgctgc cgacttatct ggtagtccag gtacgtggcg aagcgcacga tcaggaattt
    22381 actatccact gccaggtcag cggcctgagt gaaccggtgg ttggcacagg ttcaagccgt
    22441 cgtaaggctg agcaggctgc cgccgaacag gcgttgaaaa aactggagct ggaatgagca
    22501 tcgataaaag ttactgcgga tttattgcca tcgtcggacg tccgaacgtt ggcaaatcca
    22561 cattgttgaa caaactgctg gggcagaaaa tctccatcac ctcccgcaag gcgcagacaa
    22621 ctcgtcaccg cattgtgggg atccatactg aaggcgcgta tcaggcgatc tacgtcgata
    22681 caccgggcct gcatatggaa gaaaaacgcg ccattaaccg cctgatgaac aaagcggcga
    22741 gcagctctat tggcgatgtt gagctggtga tttttgtcgt tgaaggcacc cgctggacgc
    22801 cggacgacga aatggtgctc aacaaactgc gcgaaggcaa agcgccggta atcctcgcgg
    22861 tgaacaaagt ggacaacgtg caggagaaag ccgatctgct gccgcacctg cagttcctgg
    22921 caagccagat gaacttcctc gatatcgtgc caatctctgc cgaaaccggg ctgaatgttg
    22981 acactattgc ggcaatcgtg cgtaagcatc tgcctgaagc gactcatcac ttcccggaag
    23041 attacatcac cgatcgctca cagcgtttta tggcgtctga aatcatccgc gaaaaactga
    23101 tgcgtttcct tggcgctgaa ctgccgtact ccgtgaccgt ggagatcgaa cgtttcgtct
    23161 ctaacgaacg cggcggttgt gacatcaacg gtttgattct cgttgagcgt gaagggcaga
    23221 agaagatggt tattggcaac aaaggggcca agatcaaaac catcgggatt gaagcgcgta
    23281 aagacatgca ggaaatgttc gaagcgcctg tccaccttga actgtgggta aaagtgaaat
    23341 cgggttgggc cgacgacgaa cgcgcactgc gcagtctcgg ttatgttgac gatctttaag
    23401 agtcactccg atggaaggct ggcagcgcgc atttgtcctg catagtcgcc cgtggagcga
    23461 aaccagcctg atgctggacg tcttcacaga ggaatcgggt cgcgtgcgtc tggttgccaa
    23521 aggcgcacgc tctaaacgct ctactctgaa aggtgcatta cagcctttca cccctctctt
    23581 gctacgtttt ggcgggcgtg gcgaagtcaa aacgctgcgc agtgctgaag ctgtctcact
    23641 ggcgctgcca ttaagcggta tcacgcttta cagcggtctg tacatcaacg aacttctctc
    23701 ccgcgtactg gaatacgaga cgcgcttctc tgaactcttt ttcgattact tgcactgcat
    23761 tcagtctctt gcaggtgaca ctggtacgcc agaacccgcg ctgcgtcgct ttgaactggc
    23821 actactcggg catctgggct atggcgtcaa ttttacccat tgcgcgggta gcggcgagcc
    23881 ggtagatggc accatgacgt atcgttatcg cgaagaaaaa gggtttatcg caagcgtcgt
    23941 tatcgacaat aaaacgttca ccggaaggca gttaaaagcg ttaaacgcgc gggaatttcc
    24001 tgacgcagac acactacgcg ccgcgaaacg ctttacccga atggcgctta agccgtatct
    24061 tggcggtaaa ccattaaaga gcagggaact gttccggcag tttatgccta agcgaacggt
    24121 gaaaacacat tatgaatgat gaggattgtc atggctgaat tactgttagg cgtcaacatt
    24181 gaccatattg caacgctgcg taacgcacgc ggtactgctt acccggatcc ggtgcaggcg
    24241 gcgtttattg ccgagcaggc gggagcggac ggcattaccg tgcatttacg tgaagatcgc
    24301 cgccacatta ctgaccgcga tgtgcgcatc ctgcggcaga cgctggatac ccgaatgaat
    24361 ctggagatgg cagtgaccga agagatgctg gcgatcgccg ttgagacgaa gccacatttt
    24421 tgctgcttgg taccggaaaa gcgtcaggaa gtaacaaccg aaggcggcct ggacgttgca
    24481 gggcagcgtg aaaaaatacg cgatgcctgc aaacgtctgg cagatgccgg gattcaggtt
    24541 tctctgttta tcgacgccga tgaagagcag atcaaagctg cggcagaggt tggcgcaccg
    24601 tttatcgaga tccacaccgg ttgctatgct gatgccaaaa ctgacgccga acaggcgcaa
    24661 gagctggtgc gtatcgccaa agccgcgacc tttgccgcta gtctcggtct gaaagttaac
    24721 gccggacacg gtctgaccta tcacaacgtg aaagccattg ccgccatccc tgagatgcat
    24781 gaactgaata tcggtcatgc cattattggc cgtgcagtga tgaccggact gaaagatgcg
    24841 gtggcagaaa tgaagcgtct gatgctggaa gcgcgtggct aatggcaata ttaggtttag
    24901 gcacggatat cgtagagatc gctcgcatag aagcagtgat cgcccgatcc ggtgagcgcc
    24961 tggcacgccg cgtattaagc gataacgaat gggcaatctg gaaaacgcac caccagccgg
    25021 tacgttttct ggcgaagcgt tttgctgtga aagaagccgc agcaaaagcg tttggcaccg
    25081 ggatccgcaa tggtctggcg tttaatcaat ttgaagtatt caatgatgag ctcggcaaac
    25141 cacggctacg gctatggggt gaggcattaa aactggcgga aaagctcggc gttgtaaata
    25201 tgcatgtaac gctggcagat gaacgccact atgcttgtgc cacggtaatt attgaaagtt
    25261 aaaataatac cgttctcttt aaaaagtaaa acccccgaac tggtaaggaa cgggggtttt
    25321 actttttaac tttaattttg tgcagcatgc accctcaaca acagcgatta tggatcagtg
    25381 tacttatctt ttacaagcaa cttgcaatct ttagcataaa aactcgagcc tttacgaaga
    25441 aagcaatatt gatggaaaga ttaacgtgac cgccaattcg taagtacatt aaaattggct
    25501 tcgttattga agattttgct gtgctttaca ccatgccaca gaattccccc attgaaacga
    25561 gtggtgtcgt caaagctctg gtgtggagtg cagcatgcac cctcaataac tcgcacgttc
    25621 agttttgggg agatgtaagg gctaatctga atggctgcat tccttgttta aggaaaaacg
    25681 aatgactgat tgccgatacc tgattaaacg ggtcatcaaa atcatcattg ctgttttaca
    25741 gctgatcctt ctgttcttat aacacaagga aacgtactta aggtgcgtcc ggtgaaccaa
    25801 tcggacgcgc ctttaataac tataaataag tgtcttggca gatactatat aaattaactt
    25861 agtgaatgat tatgctaatg tcatcaatta aataaatata atggcgttaa ggcttcccag
    25921 taatataatt aatactctac ttccagagta gaatattaaa ttttatccgc gtggtgcatc
    25981 agcacaaatt tatcccacaa ctgttcttct gtctcgacat gcgccggatc tttcacaata
    26041 gtattgggga tcgggcacac cttctggcag gttggtgtct cgtagtgccc tacgcattcg
    26101 gtacacttat cgctgttaat ttcgtagata tgatctccca ttgaaatcgc ctcattcggg
    26161 cattcgggtt cacacatatc acaattaata cagcgtttag taattagtaa agacatttca
    26221 atggattacc gttaaatcat tttaaaatca gtaagttgta tcgagtttgt atgctttact
    26281 gtcattaact tactgtatgt tgatccagtg tatttaacct tgataaactc agtccagcaa
    26341 cacaaaaccg caacacattg cattttgtcc cgtagaaaag acttgtatgt gtgagcttgt
    26401 tttctgcgcc tacgcagata aggattgaga atgccgcgca ctgtaacaca taatccggat
    26461 agccccaata atgacgatgt tttagccgca tctgaaaaat gggacgcctg taaacccccc
    26521 tataccagcg cacacatgaa aatctgtgtt gctgccgcca aaatcatcct cgctgcttcc
    26581 ggcgtggctc gccgttccaa atacgaaaaa gagaactatc tccgtatcga tttcagcaaa
    26641 gccggtaagg ttacatttta cgccgagttt ccaaaaaaga tgggcctcaa aggtaaaaag
    26701 ctcggcgagt ggccggagct cgttatccag ctggcgcgcg aaaaagcact aggtatggct
    26761 gacggtggac tgcgggcaga gtccgtacat gcagcgctgg aaatgtaccg ggatgaccca
    26821 aagccaaagt agcccggcag aagctgagcc cggacagttt cacaacctac ggggtgcgta
    26881 tcgaccggat taaagcaacg ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc
    26941 ggctggtgga agtgctggac gagtggatcg ccactcgctc gaacaataac gccctggagt
    27001 tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc tactctttgc aacggccgca
    27061 atgttgccgc cagtctgcca gatgattatg tttcctcccg cgtacagaaa cctaccccca
    27121 cacggctttt taccgatatt gaatcaatcg cccgactctg gctcaatgtt gctgcctgca
    27181 cctctgtaca ccagaagaat gctgttcgct tcatgatcat cactggtgtt cgtccgatta
    27241 atgtccataa cctgcgctgg gactacgttt acgaggaggc tggtgaaatt gtttatccgg
    27301 aaggggttat cggcatgcga ggggctatga aaacacaaaa ggctttccgc ctgccgataa
    27361 cgcctgagat ccggcggatt atcgacgagc agaaagcctg gcgtgattca gttcctgagt
    27421 gcaacaggga ttatgtattt ttgcagccac gtgatccaat gcagccattt tcaaaacgat
    27481 cactggataa gctggtgaaa acatacagcc cggacggggc tgtaaaatga ggtgtactgg
    27541 caatagcgga cactaccatt tgttcttttt ttaagcagcc atctgatgat
//
LOCUS       DAEEDS010000053        27064 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEEDS010000053 DAEEDS010000000
VERSION     DAEEDS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27064
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.053"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..470
                     /locus_tag="KLH32_002460"
     CDS             <1..470
                     /locus_tag="KLH32_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HBE0139251.1"
                     /translation="GDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(467..607)
                     /locus_tag="KLH32_002461"
     CDS             complement(467..607)
                     /locus_tag="KLH32_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBE0139252.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            849..1142
                     /locus_tag="KLH32_002462"
     CDS             849..1142
                     /locus_tag="KLH32_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBE0139253.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1214..1873
                     /locus_tag="KLH32_002463"
     CDS             1214..1873
                     /locus_tag="KLH32_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBE0139254.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1965..2411
                     /locus_tag="KLH32_002464"
     CDS             1965..2411
                     /locus_tag="KLH32_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBE0139255.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KLH32_002465"
                     /pseudo
     CDS             complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KLH32_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3891..4310
                     /locus_tag="KLH32_002466"
     CDS             3891..4310
                     /locus_tag="KLH32_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBE0139256.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            4310..5578
                     /gene="umuC"
                     /locus_tag="KLH32_002467"
     CDS             4310..5578
                     /gene="umuC"
                     /locus_tag="KLH32_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBE0139257.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KLH32_002468"
     CDS             complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KLH32_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBE0139258.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KLH32_002469"
     CDS             complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KLH32_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBE0139259.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8062..8781
                     /gene="fadR"
                     /locus_tag="KLH32_002470"
     CDS             8062..8781
                     /gene="fadR"
                     /locus_tag="KLH32_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBE0139260.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8833..10365)
                     /locus_tag="KLH32_002471"
     CDS             complement(8833..10365)
                     /locus_tag="KLH32_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBE0139261.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10695..11993
                     /gene="dadA"
                     /locus_tag="KLH32_002472"
     CDS             10695..11993
                     /gene="dadA"
                     /locus_tag="KLH32_002472"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBE0139262.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            12003..13073
                     /gene="dadX"
                     /locus_tag="KLH32_002473"
     CDS             12003..13073
                     /gene="dadX"
                     /locus_tag="KLH32_002473"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBE0139263.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(13241..14655)
                     /locus_tag="KLH32_002474"
                     /pseudo
     CDS             complement(13241..14655)
                     /locus_tag="KLH32_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15266..17002)
                     /locus_tag="KLH32_002475"
     CDS             complement(15266..17002)
                     /locus_tag="KLH32_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBE0139264.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17097..18011)
                     /gene="ldcA"
                     /locus_tag="KLH32_002476"
     CDS             complement(17097..18011)
                     /gene="ldcA"
                     /locus_tag="KLH32_002476"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBE0139265.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            18111..18722
                     /gene="emtA"
                     /locus_tag="KLH32_002477"
     CDS             18111..18722
                     /gene="emtA"
                     /locus_tag="KLH32_002477"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBE0139266.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18724..19458)
                     /locus_tag="KLH32_002478"
     CDS             complement(18724..19458)
                     /locus_tag="KLH32_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBE0139267.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19659..19913
                     /locus_tag="KLH32_002479"
     CDS             19659..19913
                     /locus_tag="KLH32_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE0139268.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19963..21423)
                     /locus_tag="KLH32_002480"
     CDS             complement(19963..21423)
                     /locus_tag="KLH32_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBE0139269.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21360..21917)
                     /locus_tag="KLH32_002481"
     CDS             complement(21360..21917)
                     /locus_tag="KLH32_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBE0139270.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITPLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21943..22797)
                     /locus_tag="KLH32_002482"
     CDS             complement(21943..22797)
                     /locus_tag="KLH32_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBE0139271.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22797..23606)
                     /locus_tag="KLH32_002483"
     CDS             complement(22797..23606)
                     /locus_tag="KLH32_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE0139272.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23616..24374)
                     /locus_tag="KLH32_002484"
     CDS             complement(23616..24374)
                     /locus_tag="KLH32_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0139273.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(24371..25351)
                     /locus_tag="KLH32_002485"
     CDS             complement(24371..25351)
                     /locus_tag="KLH32_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBE0139274.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(25351..26373)
                     /locus_tag="KLH32_002486"
     CDS             complement(25351..26373)
                     /locus_tag="KLH32_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBE0139275.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26722..>27064
                     /locus_tag="KLH32_002487"
     CDS             26722..>27064
                     /locus_tag="KLH32_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139276.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6803 a         6586 c         7000 g         6675 t
ORIGIN      
        1 caggagacat cacgtactta cgtacagatg aaggctggct gtatctggca gtggtcattg
       61 acctatggtc acgtgccgtt attggctggt caatgtcgcc acgcatgacg gcgcaactgg
      121 cctgcgatgc cctgcagatg gcgctgtggc ggcgtaagag gccccggaac gttatcgttc
      181 acacggaccg tggaggccag tactgttcag cagattatca ggcgcaactg aagcggcata
      241 atctgcgtgg aagtatgagc gcaaaaggtt gctgctacga taatgcctgc gtggaaagct
      301 tctttcattc gctgaaagtg gaatgtatcc atggagaaca ctttatcagc cgggaaataa
      361 tgcgggcaac ggtgtttaat tatatcgaat gtgattacaa tcggtggcgg cggcacagtt
      421 ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag gcctgtgtcc
      481 atattacgtg ggtaggatca ctatggcctt tgctgaactc aacattgaca ttcttacccg
      541 cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac agccttactg atgctcttga
      601 ttttcattgg tagtctcctg ttcagaatga gtacgatgaa ctgttataat ataacaatcc
      661 cttacgggag aattccagtg gcttttctgc gttcggtaaa atcccggcgc ggcatttttt
      721 gccgggggga ttacttataa aggggcatgg actcaacaaa ccgcagaaat ctacagctgg
      781 ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga
      841 gtaaaagtat gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg
      901 tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc
      961 ctcagttagc gatgattctg ccgttggatg ggcggaagaa actggtcaat gctgatattg
     1021 aaaaggttaa actggcatta accgagcaag gttactattt gcagttaccg ccaccacccg
     1081 aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat
     1141 aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg
     1201 tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt
     1261 gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc
     1321 agtgcccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca
     1381 gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt
     1441 gattggcgcg acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca ttgccggtta
     1501 cggcgtggcg cttgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca
     1561 gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttatccccgc
     1621 ggcggaattt accggcgatc cacaaaatac aacgctgggc ctgagcgtaa acggcgaaaa
     1681 acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgttga tcgcttatat
     1741 gagcaagttt tttaccctca aggccggtga cgttgtgttg acaggcacgc ctgatggcgt
     1801 cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac
     1861 tcgcgttttg taatactttt tgccgccaga aagcggcggc aaaacttgca tcgctgtgcc
     1921 agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt
     1981 tctggcaaag taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg
     2041 gttgcggtca gtgttgcttg cataaactga tggatgaaga caccgacgaa atctacttca
     2101 ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc
     2161 gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat
     2221 ggctgccaat gacctgcgct tatcggttgc tggcggaagg taaagattta cctgcgtggc
     2281 atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata
     2341 tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact
     2401 gggcgcagtg ataaatttaa tagatgctga tgggggcttt cttaatatac gtctggatgc
     2461 tcgccatttc gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc
     2521 ttattatcaa caggtaactc tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa
     2581 ccttatacct tgagtacatg gagagaataa tcgcttatca gacttcaggt acttcaaaga
     2641 gtgtcttttt accgtgtctt ttctgatact cattacaggt gttaatcatt tttttggccg
     2701 ctgcttttag caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag
     2761 tttccgtttt tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat
     2821 cgatatcttt attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag
     2881 attttaactt gttcttcaat tctggaatca gtttcccttc aactacgccc gcagcaatag
     2941 aataggaaat gattaaacca aatggaccgg cgacgacatc ggctgcggca ccggcatacg
     3001 cttccttcct gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat
     3061 tggttaactg gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc
     3121 ttaccagcag ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa
     3181 tgaggggcgg atgctttctt ctcattgtac tcatcaaata gcaaaatata cgctgcgagc
     3241 aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc
     3301 tggctatcca taagtaaggt tttaatatcg ccgactaaaa cggaggctgc ctgtgaatac
     3361 tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc
     3421 tgatcgagat atttattata aagatctaat gctccatctg cggtttcgat tgcgttttta
     3481 actacttcta ccgttttatc tgcaacgatt tcagtcataa tcattcgcct ctttaaatat
     3541 ataaattgta atgaaactcc tgttttacaa ctattaataa attttacttc atctaattca
     3601 tagttagccg ggcgggatgc gtcaatgtct ttatttctat taatatgata aatatcaaac
     3661 aatgtttaat gtcattatgg cgaatgcttc tattctattt ttttgccggg tgatattttt
     3721 catttctgct ggatgagcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga
     3781 aaaatcagca gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg
     3841 caagaacaga ccactgtata taaaaacagt ataacttcag gcagattatt atgttgttta
     3901 tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt
     3961 gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg aatcaactgt
     4021 tgatccagca tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgatg
     4081 gtggaattag tgacggtgat ttactgattg tcgatagcgc tattaccgcc agccatggtg
     4141 atattgtcat cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga
     4201 cggtacagct tattcccatg aacagtgcgt actcgcccat taccatcagt agcgaagata
     4261 cgctggatgt ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgccct
     4321 ctgtgatgta aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg
     4381 taaaccggtg gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc
     4441 aaaggcgctg ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg
     4501 ctgtggtgtg gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt
     4561 gatgtcgacg ctggaagaac tatcgccccg cgtcgagatt tacagtattg atgaagcatt
     4621 ctgcgatctg acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg
     4681 cgcaacggtg ctacaacgta cccatcttac tgttggtgtg gggatcgccc agaccaaaac
     4741 gttggctaag cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt
     4801 ggatttatca aatctggaac gccagcgtaa attaatgtct gctctccccg tggatgacgt
     4861 ctgggggatt ggacggcgga tcagcaaaaa actggacgcg atggggatca aaaccgttct
     4921 cgatttggcg gatacagata tccggtttat ccgtaaacat tttaatgtca tgctcgaaag
     4981 aacggtgcgt gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg caccgacgaa
     5041 gcaggaaatt atctgttccc gctcgtttgg tgaacgcatc acggattata cgtcgatgcg
     5101 gcaggccatt tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg agcatcaata
     5161 ttgtcggttt atctccacat ttattaagac gtcaccattt gcgctcaatg aaccttatta
     5221 cggcaatagc gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa
     5281 cgctgctacg cgatctctgg atgccatctg gcaagcgggc catcgctacc aaaaagcggg
     5341 cgtgatgctg ggggatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa
     5401 cgcaccgcgc cccgggagtg agcaattgat ggcggtaatg gatactctga atgctaaaga
     5461 gggcagagga acactctatt ttgccgggca ggggatccaa caacaatggc agatgaagcg
     5521 agctatgctt tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaat
     5581 atagcggcag gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacagat
     5641 cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag
     5701 cgataaaaat accgagcagc cactgcggca tttccatacc taaaaaatcc cactgacgct
     5761 cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt
     5821 ccgggaaacg aaccataaaa tcacaggtgg caaatggcga aggatagagc tgaagcatgg
     5881 tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata accatcgcta
     5941 cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga
     6001 ataacgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga
     6061 accacagcgc cgtcagttcc agtgccagag cagtaaacgc catcaacagc cacgcgcccc
     6121 ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga
     6181 gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca
     6241 ataggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat
     6301 caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac
     6361 tcgacgcaga gcaagccgac gagtgtcagt acgagggtgt aaggcagggc catccacacc
     6421 atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac
     6481 gcagcctgac cgttcggcgt agcgacagag ggcagattgg taccggtatt aatggcgacc
     6541 gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg
     6601 ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa
     6661 atatagaaca gcgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc
     6721 gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg
     6781 gtgaatggca gagattcggt gaaggcttta ccgatagaat gttcatcggt gacaccggtc
     6841 aatgatgttg ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac
     6901 gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc
     6961 tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcaacacttc gcggactttc
     7021 tccggcagcg tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac
     7081 aggccacaaa tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa
     7141 tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg
     7201 ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac
     7261 tgttccagaa ccacgttgta atgcttgtcg atatgactat cgtcttgcag gtcggtgtct
     7321 tcggtacggg aagaggctac gcgatgataa ataccataaa aaccgactgc aacgctgatc
     7381 accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgctgc cgccacgcaa
     7441 aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac
     7501 agcaacagct gtttcataaa atagataccc gccaccataa acatcaataa cagcaagact
     7561 tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc
     7621 gcttcgatag ccaacagacc accggggagt agcgggtagc atttcagggc catcgccaga
     7681 gtgaaaataa attccgctac cagcaaccag cccgcgacga aagggctgat gaggaaaatt
     7741 aacgggttta cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc
     7801 aaaaaattgc gccatagcgc gcggccccag gagatctcca tgatggtttc ccttacctta
     7861 caaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag
     7921 gcaagtcttg atagtcaagg ggaaagagat gcggaaaatg aagccttgat ccctttttct
     7981 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     8041 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     8101 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     8161 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     8221 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     8281 ttctgggaaa cttccggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     8341 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt
     8401 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     8461 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     8521 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg
     8581 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     8641 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     8701 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     8761 ttagccattc aggggcgata atcccttccg tttaaagagc aaccccctca aacgaggggt
     8821 tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca actcgatgct
     8881 gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat gcacatgctt
     8941 caggacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatat aacgcagcgt
     9001 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     9061 taagttatat tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat
     9121 agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc
     9181 acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat
     9241 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     9301 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     9361 atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt
     9421 gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg
     9481 gttaaggatg gtatagtgcc agaaggtcgc acagccttcg ttcatcacct gagtctgttt
     9541 ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga
     9601 ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg
     9661 atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag
     9721 catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt tctcttcttg
     9781 cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca
     9841 cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact cggtaatata
     9901 tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac tgcggaataa
     9961 gcaattgttt ttaaagaaag agttatgtcc atagcaggcg tgtgccatca ccagcgcctg
    10021 catagtaatg gtgttctctt ccatcaagta agcgatacac gggttagagt taatgacgat
    10081 ttcataggcc agcccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt
    10141 accgaatgac cagtgcggat agttaattgg catgccgacg ctggaatagg catccatcat
    10201 ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc
    10261 cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc
    10321 gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacacg cacctcattg
    10381 ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg
    10441 cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt
    10501 ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat
    10561 attgccgcag agtcagggag atgtgagtca gctcaccata aaaaagccgt atgttgaata
    10621 atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact gtatctaccg
    10681 ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg
    10741 cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggggccg
    10801 ccctggaaac cagtgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg
    10861 cagcaccagg tgtgccttta aaagcgatta aatggatgtt ccagcgccat gcgccgctgg
    10921 cggttcgtct cgacggtacg cagtttcagt tgaaatggat gtggcaaatg ttacgtaact
    10981 gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc
    11041 gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg
    11101 ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc gatatcgccg
    11161 tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg
    11221 atcccgcgct ggcagaagta gcgcacaaac tgacgggcgg cctgcagtta cccaatgatg
    11281 aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag caggcggggg
    11341 ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag caaatctacg
    11401 gcgtgaagtt tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg tttggttctt
    11461 actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact
    11521 cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt cttgatgaaa
    11581 cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga atggcggaaa
    11641 ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc
    11701 gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc
    11761 cgatgacgcc agacggtacg ccggttgtcg ggagtacacg ctttaaaaat ctgtggctga
    11821 ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg
    11881 atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta gcgcgctaca
    11941 gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc taaggaaacg
    12001 agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca
    12061 ttgtccgcca ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg
    12121 ggcatggtat tgagcgtatc tggagcgcac tcggggccac cgatggcttt gcattactta
    12181 acctggaaga ggcaataacg ttacgtgagc gcggctggaa ggggccgatc ctgatgctgg
    12241 aaggattttt ccatgctcag gatctggaga tttatgacca gcaccgcctg accacctgcg
    12301 tacacagcaa ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca ccgttggata
    12361 tttatcttaa agtgaacagt gggatgaatc ggttaggctt ccagtccgat cgcgtgctta
    12421 ccgtctggca gcagttgcgg gcaatggcga atgttggcga aatgaccctg atgtcgcatt
    12481 ttgctgaagc ggaacatcct gatggaattt ccggcgcgat ggcgcgtatt gagcaggcgg
    12541 cggaggggct ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg tggcacccgg
    12601 aagcgcattt tgactgggtt cggcctggca ttattttgta tggcgcttcg ccgtccggtc
    12661 agtggcgtga tatcgccaat accggattac gtccggtgat gacgctaagc agtgagatta
    12721 ttggtgtcca gacgctaaaa gcgggcgagc gtgtgggcta cggcggtcgc tatactgcgc
    12781 gcgatgaaca gcgaatcggc attgtcgccg caggatacgc cgacggttat ccgcgccatg
    12841 cgcctaccgg tactcctgtt ttagtggacg gcgtgcgcac catgacggtg gggaccgtct
    12901 cgatggatat gctggcggtt gatttaacgc cttgcccgca ggcggggatt ggtacgccgg
    12961 ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg
    13021 gctatgagtt gatgtgcgcg ctggcgttac gcgtcccggt tgtgacagtg taacttgttg
    13081 taagccggat cggaggcagc gtcttctggt tgcgaaaaaa tcatccatcc ggctggtcag
    13141 caactgtagt tgttaatgtg acagagccat tgcccatgat agtgtccatt aaaagaatgg
    13201 acactatttc cccggaacct gaactcgccg cacaggcgtt ctacataaaa cgcttacgct
    13261 tcattgttga ctcggagtcg acttcagata aatcacgctt cacccttgat gcagattcgg
    13321 gttcatgttc agattgttga ttaacaggct gcgagtcctg ttgtctttcc agactcaaca
    13381 ggcccaactg ttgatccagg agctccaggt cctcctgtac cttcacgcgc tccaggagtt
    13441 ccgggtcatc caggaactcc agatccataa ggtcctccag ctcctcgagt ccttccaggt
    13501 caacctagcc ttccttgtgc aaaaagtcca aaaggtcctt caggccctgc tgttttttca
    13561 ggccctcgac ggctttgagc agcgtcggta aattgtcttt gtcccacggg ttaccattac
    13621 agtcaaacac cttcaggctc tggggctgcg gcacctgtac cgttttaaac ttgttttctc
    13681 cacagtagta aattattagt ttgggcggca gccgtggtga agagatcaaa tcgttactag
    13741 aacagtcaaa tactttcagg cgagtcaagt tctccagcat ctcatcaggt attgagttaa
    13801 tattgttgcc ggcacaggat agtttataca ggtactgggg catcgacggc ggtaaaaatt
    13861 gcaagaagtt ttcagaacag ttaatcactc tcaggttagt cgacagctca ggcagccaga
    13921 taatactgtt ttcatagcac ttcagtacca tcagttttga gggctgcacc ttcggtaaca
    13981 agatcaagac gttatctgta cagtcaagca ccagcagcca ctttggcatc accttcggta
    14041 acgcggtcaa cacgttttta ctgcagtaaa attcaatcag atgtggaggc aaattctcag
    14101 ggagtgaagt caatcccagg ttgctaagat tgagcatact ccgtcttgta atgagacatt
    14161 ccttcattct gcgcaatgct tctgcccggt tttcaatccg gcttgctggc aggttttctt
    14221 ccaggatatt ttccttgatt tcttccagac tttctttcag gtcttcttgg ggaatttctg
    14281 actgtttgtt ttgctggttt tcttccagga tttcgtacag gtttttttgc aggttttctt
    14341 gcaaaccatc ctgctgtata gcccattcgc accatttggc gaccatcatg aactcgttat
    14401 gttgctgatt cagacgagtc cccaacgtga tgatggtatt gttgtccacc tgtatgttca
    14461 ctttgcccgc gatactgagg tcaatgatct gccagggggc atcaccccag ctgaattttt
    14521 gcgggctgag atacttcatg ttctgcaaaa tttgattctt gagaagagcc gccctggcgg
    14581 gcgtcgtacg cctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc
    14641 tttaaagcta gccataatgc tacgtttcct tgaaataaat atcctctggc agcgctggag
    14701 acgtgaatgt ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc
    14761 atcgttgact ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg
    14821 gcctaggtag ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt
    14881 tattaagcaa aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga
    14941 aacgcctatt gtgtgggcta tgtcagtgcg gcaggttgcg tgggggaata tgttcaggcg
    15001 atgtcaggca ggagcccctg atggttgacc ggtaatgcgg ggctggtttt tattacctgt
    15061 tacagtcgga aaaagccctt acgctgactt tcttgagccg gtgttttgag ttcattagcc
    15121 agagcctgca atggtttgtc atccataatt taaccttctt ctgatgttgg gatgagcata
    15181 tcaaaatcag gaaatgacaa gtttattaca ggctcaggct gcgttcagta aagcatcgca
    15241 tccggcgata gatgctgcat taaatttaag attcagcttc ctcttcagcc acccgaacgc
    15301 caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact
    15361 ctacctggtc accgacaacc ggtgccgcgc ctagcaactg ctggacaatt tcacccagcg
    15421 tttgctgttt atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag
    15481 catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg
    15541 gtggcgactg gctgaacagt ttaccgagcg ccgggagatc gcgttcccga ccaattacac
    15601 acaatacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac
    15661 gaaacagggc tgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcaccta
    15721 cacaccattt atcagcactc aattgataaa caaactgctc ccacggattt tccggatgaa
    15781 tatccaggcc aacgcgtgac accggacgtc cgacgggcgg aaccaccact ttggcttttt
    15841 tcgccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg
    15901 cgacattaaa gaacagtcgc gcattctcca gccccgccat catcgggaac acggctagga
    15961 tgattggcac cgcgccgcgt aatcccaccc agctgataaa cacgcgctca cgcagattga
    16021 aaccgcggaa agggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc
    16081 atgccgacaa aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata
    16141 gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca
    16201 ggatgccgta gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag
    16261 ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta
    16321 atgccagcaa tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta
    16381 aataaccacc gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca
    16441 cgaacatcca actgacactg ctttcatgct gctggatcat tgcaatcagg gtaatcgtca
    16501 gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt
    16561 cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggtg gagccgacga
    16621 tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc
    16681 cggttaaacc agaggtgata agtacgccca gcgtcgccag cgacagcgcc ggtcctaacg
    16741 ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg
    16801 ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat
    16861 cgactcccgc cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg
    16921 aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc
    16981 taattattgt tgtggcatcc aacggtcggt tactcctgat tacgctgtct cttatataaa
    17041 ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac
    17101 attttaagaa caggatgacc gctaatcgtc aactgagcgc cttcccgggt gttattcaga
    17161 atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg
    17221 gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa
    17281 cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga
    17341 cgtggcaaaa ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc
    17401 acgttaatat cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca
    17461 atcagtgaaa tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc
    17521 gggccttccc cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg
    17581 tgctcggtaa aggcgttcag ttcatccgca ccaaagtttg ctaccagcat ggggccgcta
    17641 aaggtgatga cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt
    17701 ccgcaaatga gtaacgggtc atgttgttgg cgagccacca gtgcctgcca gtcaatatct
    17761 gccagtaaac gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggta
    17821 gtcagtctgg caagggaatt gagatcttcc agacgctccg tttccgtacc cgcaaaacgc
    17881 tcacaacgac gggcaataac ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc
    17941 tggataccac gcagcgcggc gtgctgttta atgctgtaac ccgatggggc aattaagtga
    18001 aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag
    18061 gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga
    18121 gatggtttgc ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga
    18181 acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc
    18241 agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg
    18301 aatcgggtgg taatccgaac gcggtgagta aatcgaatgc cattggtttg atgcagttaa
    18361 aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga
    18421 ccagcgagct gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc
    18481 tggaaaccgg cccgctggca ggcattgaag atccgaaggt actgcaatat gcgctggtgg
    18541 tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg
    18601 cgatcagcaa aatcaacgat ttggatgctg acgagttcct cgatcacgta gcgcgaaatc
    18661 atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt
    18721 aaatcagtcg cgcactttgt ccgctttttc ccgggcttct cgctcgagag agaaaataat
    18781 ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg
    18841 agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctggtgg agattaactg
    18901 ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatttgag cgaagcgcat
    18961 accttcttgt aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga
    19021 caaatcatac aagcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata
    19081 aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt
    19141 gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct gtagttgttc
    19201 aacagtaaac tcgactttcg caccctgagt ttcggcggta atggtaatgt gctgtgcctt
    19261 tagtacggcg atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt
    19321 tattgccagt attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc
    19381 ggctttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg
    19441 ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg
    19501 tcatagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc
    19561 agccgacaaa cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc
    19621 atttcttgcg accgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta
    19681 tttttggcct gatagccggc attatcggca agctaatcat gccggggcgt gatggtggtg
    19741 gatttttcct gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga
    19801 ccatgtttgg cattgacggc tccatcagcg gttttaattt gcacagcttc ctggtggcgg
    19861 tggtgggcgc tatcctggtg ctgggcgtat tccgcctcct gcgaagagaa taagattttc
    19921 ataaggcgga tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac
    19981 acctgcccag aaattgcgac cttcttcaat aaacccttca ctgtaggcat attgggtatc
    20041 aaacaggtta ttgaccgacg cattgacgct gaagccatga cctaaggtgt aatcggctcg
    20101 aatgtgggtc accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga
    20161 ggaacgcgcc tcttccgaca gcgttacgct taacggctcc catggtttga gtgtcatcca
    20221 tgcggtcatt gtctgcgttg gaagatcggt tatcttgccg atgtctttgc gtttggcgtc
    20281 agcgtggatc agagcgtagc tcaatcctac atccagtata ttgctgattt tccccttaat
    20341 accggcatcc agaccgctgt aatccaccgt gccgctgttc tgattttgaa tggtatcggc
    20401 attgatattg tgcgagagga tggcatcact cacccggtta tagtaaacgc tgacctcaaa
    20461 tccccagtcg cgcgtgaagg caccattcca ggttaaatcc accccgcgag cgcgttccgg
    20521 cttgagctgc gggttaacaa tcgctatctg gttatacgca ggtttggatg tggtatagcg
    20581 ttctttcagc gtcggaaagc gtgtgcggtc atagtacgaa agcgccagag tgtcttcatt
    20641 ggcaaagtgg tatttcccca tcacctgcca gttaaaagct gactgattgt tgtcgtcata
    20701 atgggtgatg ctgccatctt tctcgtgttt tttcgcttct acgctatcgc gccagtcata
    20761 gctgattccc gccacgacat cgacattatc agcagcagcc cattgatatt cactggcgag
    20821 cgaccaggta cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg
    20881 tacgtcatct ttccagttaa cggcaaacga cagcagatcg ttttcacgca catcggctgc
    20941 cagttgtaat ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc cttttttatt
    21001 tttcaaatca gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag
    21061 ccgacttttc agggtaaaac ggtcgtttag ttgggtcgct ccctgataat aaaaactttc
    21121 tttgtcatac tctggccact gccagtagcg tgatttttga ccacaatttc cgctgtatgg
    21181 cgggttatct ttttcaccat cctgcttaat gtaagtcagc gtgtattcat cgttttcgcg
    21241 cggtgtaaaa ccgagcttca caatgccgcg tttatcatca gccgatgaat taatcatctt
    21301 gccgtgtttg cctgcaatat cattatttac accatgcggc aggccgagaa aatcctgctt
    21361 tagctggcta ccgctgactt gcaaataccc aagatcgctg ctggcggcaa atgaagcatg
    21421 catatcgtag gcattgtcct gactacggct ccatccctgg cgatatccca gacttgcttc
    21481 ttcagggggg tgatattaat ggctccgccc atctgattag gcccatgaag cagcgacgaa
    21541 taccctttgg aaacttcgac tgcccccaga ttgttggtca gaatccgcgc cagatcgagg
    21601 ttgccgtcat aggggacata aatgggcaca ccgtcgaaat agaccggcac ctgccgacta
    21661 tcaaagccac gaacttttac ctgttcttcg ttgcggctgc ctgacttttg cagcaccacg
    21721 ccggggacga cacttaatgc ctgagcgaca ttctgtttat ccaaggcctt catggtgggt
    21781 tgatcgagaa cggtggtcgt cgtcgatgat accggactgg accatacggt cagcgtgtcg
    21841 ctctcctgag ccgctacggc ctgctgggta aaggcaagtg ataatgctgt gcagagataa
    21901 cgttttttga gtctcataga ataaattccg tttaacaaag aattaaatag aggctgtggg
    21961 ttgcagactg actttaatgt cagctggtgc cgcgtaataa ggcgcggagg taataaaaag
    22021 gcgaatgccg cagtcgaggt aatttttgag tgtcgtcaga ttaattccgc cggtcagagc
    22081 cagcgtgcag tgaggagcca gcgacggcgc tatttgcgca atttcagttg cctgttgcgg
    22141 actaaatttg tcgagctgaa gtacgtctgg ttgtgcgcgt aacgcggcga tagcctcttt
    22201 cggcgtgtcg gcttcaacaa caattttctt ctcgggtgcg tggcgacgta actgagcgat
    22261 tgcgcctgac cagccctgat tgtcatgaag aaaatggcgg tggttggcaa acaataatat
    22321 ggtttccgca catccggcac gatgaattag tcctccggca gccagaattg cctgcgaagc
    22381 cagtaagcgg gtgcccggaa ttgcttttcg ggtgcaggcg atattaccgt caggataacg
    22441 ttcacgaagc aatgccagca tttgagcgag ataatcagaa acaccgcaac tccactccag
    22501 cacattctgg actgccttcc atccctgatg aagtgctgcg gcattaccct gcgcacggat
    22561 tagccgttga cccgcgttcg cttgtgaacc gtcgctgacc gcgtcatcaa tagttaatcc
    22621 cagcgtagtt aacatcttac acgcgacaga aacgccgctg acgcagccgc cctgacggtg
    22681 aaaaaacgct atatagccag gctggtgtcc aatatttaat gctcgcgtgg tcaggtcacc
    22741 tccctggata tcttccagca gtaacgcatc gatttgtgcc tgagaaagga aaatcatcac
    22801 agtgcctcct gcggtacgct atcccaccag atgagcgccc agtcgcggct ggcaggggcg
    22861 agggcacgag catcttgttg ttgataccag tgatataacc gctcacgctc atcgtcgtta
    22921 agcgcgccaa gactccagct gacgttctgc tcaaaacgtt cccaggtgct gttatcctgt
    22981 tggcaatttt gtccacggat aaaatcaacg tgggcataaa ttcccatctg atacaggacg
    23041 ttgagcgcaa agatatagtt gggtaattcg atcacttccc gaccagccgc ccgctgaatg
    23101 gctggagaga cgaacgaggt gctgaccaga tgagttgtat agacgcgaag ccgtgcctga
    23161 ttattcagtt tgctcattgc atggcgcata tcagccacca gagtggagcg ggaagccacg
    23221 gcaatatcgc agcgtggcag gtcactccag tcctcttccc aggcgcgctg gatccagtgg
    23281 acattgtcag ccttcagtgc ggcggcgcgg cgggcggcaa cgttcagcat cccctgactg
    23341 taatcaacgc cataaaccgt agtgagtttg tcagccagcg ccagcgatac ggtgcctggt
    23401 ccgcagccca tatcgaataa ggtctgagcg ccttgcaggt caattttagc gatcagttgt
    23461 tgcaggtagc tgtccgttgg cgaggcgcaa ttttccgcca ttttctccgc ccgttgatcc
    23521 cagtgatcgg gcgttttttc tgtgcggtga gccagtttga gttgctgcag ataaaggtct
    23581 gcaaaatcaa tatcatcaat gagcatgatt gttccttagc ggctaattgc agagaggtga
    23641 tgatgaattt ggtcggcaga aacccgatac agggccgcca gtttgttcgt tgttaactgt
    23701 tcggtcggta agccttgtgt tactcgtcca tctggctcta cttggataat gctgtcggca
    23761 atggcgttgg catggagcgg atggtgagtc gacattaaca ttgtcatgcc gttatttttg
    23821 agctgcgcca gcgtgtccag aagttggatc tgatgaccga aatcgaggct ggaggcgggc
    23881 tcatccagta acaacaagcg cggttgttgg acgagtgcac gggcgattaa agccagctga
    23941 cgttcaccgc cgctaagcgt attccagcgt cgtgtcgcca gatgcaaaat gcccagcttt
    24001 tccagttgct ccgtggcttt caggcgttct tgcttaccgg gaacggaaaa agcgcctatg
    24061 gtgggggcca ggcccatcaa caccatatcc agcacactga aagcaaaaat accgtcgtgc
    24121 gcctgcggca cccaggcaat agcctgtgcg cgctggcgat ggctgagtgt ctgcacagag
    24181 aggctatcca gtaggatctc gccttttaat aacggtataa cgccaagaat ggagcgcatg
    24241 agtgtggttt tgccgcaacc attcgcgcca agcagacaac aaattgttcc ttgcgacagg
    24301 gtgaaggaca cgtcgcgtag cactggatgg cgggagtaac ccagagacgc atgacgaacg
    24361 gcaagcaaag tcactggcga cctccgcgta acaacaaaag caggaaaaac ggtgcgccaa
    24421 caaaagcggt cagaatgcca agtgggattt ctgttgttcc aatgctgcgc gccagcgtat
    24481 cggtaagcag gagcagaata gcaccagtac acattgccat aggcaacagc tgctgatggt
    24541 tatgaccggt caacaagcgg gcaatgtgcg gaacgaccag accgatccaa ccaattatgc
    24601 ccgctattgc taccgtgctg gcggtgatta gtgtggcgca gacaatcaat ccaaagcgca
    24661 gacgcgtcac gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg agtaaattca
    24721 tgcgccagcg gagaaaaaac aggggcaagg agcctatgag aatgatcggt gcagcatagc
    24781 agagatcgcg taaggtcacg gtggacagtc cccccagcaa ccagaaggta attgacggta
    24841 actgggtgta aggatcggcc agcgttttaa ttagcgatat ccccgctccg cacaacgttc
    24901 ctaacgcaat gcctaccagc accagggtta agatcggatc gtggcgggtc acccgacgcg
    24961 taatcaacca gacgcccgcg accaccatca gcccgccgca aaatgcgtac agctggatgt
    25021 aaacaatggg taatccccac aggattgccg cgcaggcacc aagtccggca ccggcggaga
    25081 cgcctaaaat atccggtgaa actaagggat tacgcaacat tccctgatag gtggttccgg
    25141 ctcctgccag cgctgcgccc agtaacagcg cagcaagaat acgcggcacg cggatttgcc
    25201 agaagacaat ttgttcctgc acagactcat tttcttgacg aagaattagc gccagcagtt
    25261 gctgcatatc gagatgataa gccccactta cggcggcaaa agtgatactg atagctgcca
    25321 gcagcaaaat acatccgttt acgattctca ttagctcgcc accaattttt gatagtcggc
    25381 atcactgagt gaacaatgcc agaacagttg ggcgtaatgc tgcatgtcac ttttaaactg
    25441 gcgattgatg gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa gacggttgat
    25501 tcccggcggg gcatcgagcc agccaaaggg caagccactt aaaaataaga tgcgttgttc
    25561 cgcaacggct ttcacgccct gccagagagg atcacgacga ataaaatctg cagtaacggc
    25621 ctcctgaacc agaataatat ccggctgcca gcgcaggaga ttttccatgg aaacctgtgt
    25681 caggccgtgg cgatcggcta tttgcgcgac gttgtgcaaa cctaataatt ccgccgcctc
    25741 ggtatgcaac gaaccctgta agcccgtttc cagccctcgg ggaccgcgcg cagcataaaa
    25801 gcggaggtta gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga agtggctggc
    25861 gagattggct tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa gcgttgttaa
    25921 ctgttctgct gattgcgcca gttttccgtt aagcaataac caggggattt gtgtctgttc
    25981 gctaacctgc cgtgcctggg agatcaaggt ttcatccgta ttgccgcaat caacgaccaa
    26041 atcgggatgt aacgccataa gcccttccag cgatagtgtg ctggcgcgcc cggcgagtcg
    26101 tcccagcctg ggtaactggc gaatgtgctc tggcaatgga attaatgcct gacgggcaaa
    26161 atcaaaagag gaaaaaccga ccattttttc tggcgcgaca gccagcaaga gcaaatcggc
    26221 gggcgcgcct gcgctgacga tgcgtgtaat gttttgggct tcgggaagag attgcgaagc
    26281 aaaacgatta actgtttgtg aaaaggacgg tagagactgc aggagcaggg tagatgcaag
    26341 tgcctgcgca aacgtccgtc tggttatggg catggagaac atcctgaaaa gtaaaatcga
    26401 tatatacttt catatatatc gataaaaatc atgttcaata tcaaagttta gctattgaaa
    26461 acatgaagat gtgacgactt ccgcaagcct gttgaccctc tctatcatta aaaatgtatt
    26521 tatcaggtgg ctaatattat ttaaatcatt taaggctaaa tcatttaaat atgaatgaca
    26581 ttattgtttt ttatcgtcca cggttacgcg acgttgaagt tacttgtttt ataatgagcg
    26641 atttttgata tctcgccgag aaaattatca gcattctctc gtttcagtgt cgtgaatata
    26701 ctgttaacgg aggtgaaaag aatgctgggg gttatacaaa acctatgttg ttgtcgtccg
    26761 gattcaccgc ttgctgatag taagcagcgt aaccagaagt taccgcaaca agactttgag
    26821 gattttaaga acgacgttat taatgtcatt aacaacaatg aagatattta ttttcttaga
    26881 gagagtaatt tactgaatgc gtttattcgg acgtgcaaaa aaagagatga taacagttac
    26941 ggaaatatga tatttacgct ttattttgat aacgatatct atgggaacag aattatcaaa
    27001 gcatcgctga acggcggaga gttgcagtta ttaagcacag ggtgtagcaa tgataacatt
    27061 ctcc
//
LOCUS       DAEEDS010000054        26076 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEEDS010000054 DAEEDS010000000
VERSION     DAEEDS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.054"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KLH32_002488"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KLH32_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBE0139277.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="KLH32_002489"
     CDS             complement(2138..2584)
                     /locus_tag="KLH32_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBE0139278.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="KLH32_002490"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="KLH32_002490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBE0139279.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KLH32_002491"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KLH32_002491"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBE0139280.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="KLH32_002492"
     CDS             complement(5347..6009)
                     /locus_tag="KLH32_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBE0139281.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="KLH32_002493"
     CDS             6182..6595
                     /locus_tag="KLH32_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBE0139282.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KLH32_002494"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KLH32_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBE0139283.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KLH32_002495"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KLH32_002495"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBE0139284.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="KLH32_002496"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="KLH32_002496"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBE0139285.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KLH32_002497"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KLH32_002497"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBE0139286.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KLH32_002498"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KLH32_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBE0139287.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="KLH32_002499"
     tRNA            complement(9861..9948)
                     /locus_tag="KLH32_002499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="KLH32_002500"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="KLH32_002500"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBE0139288.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="KLH32_002501"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="KLH32_002501"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBE0139289.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="KLH32_002502"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="KLH32_002502"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBE0139290.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="KLH32_002503"
     CDS             14005..14592
                     /locus_tag="KLH32_002503"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBE0139291.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="KLH32_002504"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="KLH32_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBE0139292.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="KLH32_002505"
     CDS             14984..15841
                     /locus_tag="KLH32_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBE0139293.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="KLH32_002506"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="KLH32_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBE0139294.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVVTGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="KLH32_002507"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="KLH32_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE0139295.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="KLH32_002508"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="KLH32_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBE0139296.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="KLH32_002509"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="KLH32_002509"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBE0139297.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KLH32_002510"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KLH32_002510"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBE0139298.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KLH32_002511"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KLH32_002511"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBE0139299.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="KLH32_002512"
     CDS             complement(22898..24037)
                     /locus_tag="KLH32_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBE0139300.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="KLH32_002513"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="KLH32_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="KLH32_002514"
     CDS             complement(25322..26017)
                     /locus_tag="KLH32_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0139301.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6647 c         6906 g         6306 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggttac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcgga
//
LOCUS       DAEEDS010000055        24958 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEEDS010000055 DAEEDS010000000
VERSION     DAEEDS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24958
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.055"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(52..276)
                     /gene="cspD"
                     /locus_tag="KLH32_002515"
     CDS             complement(52..276)
                     /gene="cspD"
                     /locus_tag="KLH32_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBE0139302.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            599..919
                     /gene="clpS"
                     /locus_tag="KLH32_002516"
     CDS             599..919
                     /gene="clpS"
                     /locus_tag="KLH32_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBE0139303.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            950..3226
                     /gene="clpA"
                     /locus_tag="KLH32_002517"
     CDS             950..3226
                     /gene="clpA"
                     /locus_tag="KLH32_002517"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBE0139304.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(3629..3716)
                     /locus_tag="KLH32_002518"
     tRNA            complement(3629..3716)
                     /locus_tag="KLH32_002518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3680..3682),aa:Ser,seq:gga)
     gene            complement(3970..4188)
                     /gene="infA"
                     /locus_tag="KLH32_002519"
     CDS             complement(3970..4188)
                     /gene="infA"
                     /locus_tag="KLH32_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBE0139305.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4473..5177)
                     /gene="aat"
                     /locus_tag="KLH32_002520"
     CDS             complement(4473..5177)
                     /gene="aat"
                     /locus_tag="KLH32_002520"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBE0139306.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(5219..6940)
                     /gene="cydC"
                     /locus_tag="KLH32_002521"
     CDS             complement(5219..6940)
                     /gene="cydC"
                     /locus_tag="KLH32_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBE0139307.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6941..8707)
                     /gene="cydD"
                     /locus_tag="KLH32_002522"
     CDS             complement(6941..8707)
                     /gene="cydD"
                     /locus_tag="KLH32_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBE0139308.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8830..9795)
                     /gene="trxB"
                     /locus_tag="KLH32_002523"
     CDS             complement(8830..9795)
                     /gene="trxB"
                     /locus_tag="KLH32_002523"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBE0139309.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10339..10833
                     /gene="lrp"
                     /locus_tag="KLH32_002524"
     CDS             10339..10833
                     /gene="lrp"
                     /locus_tag="KLH32_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBE0139310.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10968..14996
                     /gene="ftsK"
                     /locus_tag="KLH32_002525"
     CDS             10968..14996
                     /gene="ftsK"
                     /locus_tag="KLH32_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBE0139311.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            15151..15762
                     /gene="lolA"
                     /locus_tag="KLH32_002526"
     CDS             15151..15762
                     /gene="lolA"
                     /locus_tag="KLH32_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBE0139312.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            15773..17116
                     /gene="rarA"
                     /locus_tag="KLH32_002527"
                     /gene_synonym="mgsA"
     CDS             15773..17116
                     /gene="rarA"
                     /locus_tag="KLH32_002527"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBE0139313.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            17207..18499
                     /gene="serS"
                     /locus_tag="KLH32_002528"
     CDS             17207..18499
                     /gene="serS"
                     /locus_tag="KLH32_002528"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBE0139314.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            18739..21183
                     /gene="dmsA"
                     /locus_tag="KLH32_002529"
                     /pseudo
     CDS             18739..21183
                     /gene="dmsA"
                     /locus_tag="KLH32_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            21194..21811
                     /gene="dmsB"
                     /locus_tag="KLH32_002530"
     CDS             21194..21811
                     /gene="dmsB"
                     /locus_tag="KLH32_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE0139315.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            21813..22604
                     /locus_tag="KLH32_002531"
     CDS             21813..22604
                     /locus_tag="KLH32_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBE0139316.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(22640..23266)
                     /locus_tag="KLH32_002532"
     CDS             complement(22640..23266)
                     /locus_tag="KLH32_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0139317.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            23581..24729
                     /locus_tag="KLH32_002533"
     CDS             23581..24729
                     /locus_tag="KLH32_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139318.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         6223 a         6163 c         6644 g         5928 t
ORIGIN      
        1 ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac
       61 tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg
      121 gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt
      181 ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt
      241 ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt
      301 atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact
      361 ctagaaatac aatttagaga attagggaga gccgtcaagc atttgacagg ggccaggggg
      421 caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg
      481 ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa
      541 gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat
      601 gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc
      661 gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga
      721 gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt
      781 gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc
      841 agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg
      901 tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca
      961 agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt
     1021 tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct
     1081 ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca
     1141 aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt
     1201 tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac
     1261 cggtgcaaac gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt
     1321 gcgtaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa
     1381 agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg
     1441 tggggaggaa cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg
     1501 aatcgaccca ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg
     1561 tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc
     1621 agaaggtctt gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac
     1681 gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga
     1741 aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat
     1801 tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc
     1861 taacctaatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta
     1921 tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat
     1981 tgatattact gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa
     2041 gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct
     2101 ggcggtgaaa tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga
     2161 agcgggcgct cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc
     2221 ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag
     2281 tgaccgcgat accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga
     2341 taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga
     2401 acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt
     2461 gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta
     2521 tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga
     2581 tcagggcggt ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga
     2641 cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg
     2701 tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac
     2761 cactaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa
     2821 cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct
     2881 cgacaacatt atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa
     2941 attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca
     3001 ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat
     3061 ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc
     3121 gctggtggac ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta
     3181 cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt
     3241 aggttggtga agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac
     3301 ggtgaaaacc ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg
     3361 taaggataaa aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct
     3421 aaacgtgtag tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt
     3481 aaaacctttg cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata
     3541 ctccacccgc ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg
     3601 cactttcgta cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata
     3661 cgttgccgta tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat
     3721 tgttttgcta ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa
     3781 acggtcaaga agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca
     3841 gtatatctac atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac
     3901 aaaataaaaa ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg
     3961 gtaaaacaat cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag
     4021 ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga
     4081 gatgtgtgca gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa
     4141 cgtttcaaga acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg
     4201 ggtatcacta ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag
     4261 atttgcgcgc taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa
     4321 gccgcaccaa aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac
     4381 gcgaaaaatt ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta
     4441 taacactctg gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt
     4501 ggtacccaga aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa
     4561 tcccggcggg gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag
     4621 tcgataagct taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta
     4681 gacgcatttt ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg
     4741 ccgtacatac cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg
     4801 agttcgtgaa ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg
     4861 cgatcgctgg cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag
     4921 ggcgagcgtt tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc
     4981 accgcgcggg gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa
     5041 ataccacgct ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc
     5101 aggccgttag gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa
     5161 agctgaacca agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt
     5221 acaaaccctg cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac
     5281 cttgctcaat aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc
     5341 gaagtcgatg ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa
     5401 ggatctggct ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg
     5461 gcgcatcatg taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct
     5521 ggcgtccgcc ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac
     5581 caacgcgacg caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca
     5641 gattatcacg cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct
     5701 gtcgtagagc cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct
     5761 gttgcgggtc ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc
     5821 gcccgagaat cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct
     5881 gttgggattg ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg
     5941 caacacgagt ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga
     6001 tacgtacggc agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca
     6061 gtgcttcaaa cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag
     6121 cattgccgcc aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa
     6181 tgagcagcat tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca
     6241 gccattgaat ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg
     6301 tcagctcagc ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat
     6361 gagtcagatt ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa
     6421 gcgtcagtaa cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc
     6481 cgattgtcac caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt
     6541 aaagatgatc gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat
     6601 agcgcgccag tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat
     6661 gctgcaacac gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag
     6721 tacgggtgat tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca
     6781 gtccggcaac ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac
     6841 cgatactggc gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat
     6901 gacgtttata cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat
     6961 gggccagtaa tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct
     7021 caataatccg gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact
     7081 ggtgggtgac cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc
     7141 gctgttcact gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg
     7201 gatttagtaa cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag
     7261 cctggtcgcc aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc
     7321 aggcgttatc cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta
     7381 cgttatcccg caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt
     7441 tacgccatga ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct
     7501 gatatgagag aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc
     7561 caaccaacac tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt
     7621 taccttccgg cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg
     7681 ctaattccgc ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac
     7741 tgtcagctgc accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg
     7801 gctggaaaaa ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag
     7861 tatcgtagtg accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca
     7921 gagcaattga cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta
     7981 gcacttccat tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag
     8041 cttcaccacg accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc
     8101 cacttaagcg agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa
     8161 ccagcgccat aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc
     8221 agttagaggg gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt
     8281 gcggcagata gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg
     8341 cccagctccc cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga
     8401 gaacctgacg gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc
     8461 gtaaccagac cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca
     8521 gcagggcttc acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc
     8581 aggcctgggc aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc
     8641 aacgttggga gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt
     8701 tattcattgc acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg
     8761 gggaaaaaat aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga
     8821 tttgtaaaat tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc
     8881 catgcagcca gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc
     8941 tgcggcaaag acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac
     9001 tttgatgtag ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg
     9061 accgatagca acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt
     9121 atcgcgcaga cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg
     9181 gttggtgtgc agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat
     9241 tttttccgcg cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga
     9301 cagatacagc gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt
     9361 gcgatagaag aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc
     9421 ttcagagggc aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca
     9481 agtgtattcg gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt
     9541 gatatgatca aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat
     9601 taataacgga ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt
     9661 ggtcagttgg ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc
     9721 cgcgtagaca gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg
     9781 tttggtcgtg cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga
     9841 atttacagac gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca
     9901 tcgataagca ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt
     9961 ataagccgat taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta
    10021 aaaatcgatg ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa
    10081 aaaacagtat tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt
    10141 gaaatctacg tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa
    10201 cggtcttctc accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat
    10261 cgtgacgggt agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa
    10321 tacagagaga caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt
    10381 atcgatcgta acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt
    10441 tctaaacgtg tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga
    10501 caagggttta ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt
    10561 ctggtattcg ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat
    10621 accgctgtac aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac
    10681 tacctgttga aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc
    10741 ctgctgcgtc tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag
    10801 cagagtaatc gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat
    10861 tttgattaca ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt
    10921 ccgttttagc atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat
    10981 acactgaaga caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg
    11041 cattgctgat ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta
    11101 acccttcgga ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg
    11161 ggatgcccgg tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca
    11221 ccattcccgt cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg
    11281 aatacattga ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta
    11341 cctcctgtgg tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg
    11401 tcattggcag cttactaagc actacgctac aaccactgct acacagtagc gggggaacta
    11461 ttgcgctgct ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga
    11521 ccattgctga aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta
    11581 cccgtcgcga tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag
    11641 atgaaaatca cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag
    11701 cgcgtcgtaa acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg
    11761 cgttgttctc cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg
    11821 tggctgccga cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg
    11881 acgaatacga tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg
    11941 ctgctaccac ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc
    12001 ctgttgcctc tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg
    12061 gtccacaaac gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac
    12121 aatatgcgca gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc
    12181 agccgtatta tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc
    12241 cagaacaacc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc
    12301 cacagtctac ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt
    12361 accaacagcc gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag
    12421 agacaaaacc cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc
    12481 gtgaacgtga acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag
    12541 aaccgatcaa atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg
    12601 ctgccgctgt ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg
    12661 ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg
    12721 tcaaagaggg gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc
    12781 gtgaattggc gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc
    12841 gtgaagccca gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg
    12901 cgatgcagca ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg
    12961 aacagtatca acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg
    13021 aactggctcg tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg
    13081 gggcgaatcc gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg
    13141 atggtccaca cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac
    13201 cggttgcacc acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt
    13261 atcagcagcc acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg
    13321 ttgcgccaca gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc
    13381 aacagccgca acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg
    13441 cgccacagcc gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc
    13501 cgttgcacaa accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg
    13561 aagtggagcc ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc
    13621 tggctgattt ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc
    13681 gctttgaact taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg
    13741 accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac
    13801 cctatgtagg tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt
    13861 tggataacgc caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata
    13921 tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga
    13981 ctaccggttc cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag
    14041 cacagccaga agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt
    14101 atgaaggcat tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg
    14161 cgctgcgctg gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg
    14221 tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga
    14281 ttccagaccc gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa
    14341 aagaaccata cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta
    14401 aaaaagtgga agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc
    14461 tcgtactggc aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata
    14521 ttccgacccg tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc
    14581 aggctggcgc ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca
    14641 cgttgccggt acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc
    14701 aggactggaa agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa
    14761 gcgaaggtgg tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg
    14821 cggtgcagtt tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc
    14881 gcattggtta taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca
    14941 gcgaacaggg gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa
    15001 tgcattgccg gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag
    15061 tattttcttc tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg
    15121 atcgggagtg acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta
    15181 ctctcaagct tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc
    15241 ctggataaag tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc
    15301 gcggcggtgc aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg
    15361 catatgacac aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat
    15421 aacccgttcg ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg
    15481 tttatgctga ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat
    15541 ggcgatgact ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt
    15601 aacgtgggac gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc
    15661 agcagttatc agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc
    15721 accccgccgc aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa
    15781 tctgtcgctc gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga
    15841 aaatttagca cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg
    15901 cgctatcgaa gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa
    15961 aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc
    16021 cgtcacctct ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa
    16081 tgcaggtcgc cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca
    16141 ggatgcattt ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga
    16201 aaacccgtcg tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa
    16261 atccctgagt acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg
    16321 tggctatggt ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact
    16381 ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga
    16441 agtctatgat agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga
    16501 acgtagcgcc cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca
    16561 taagtcggta cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac
    16621 cgctggtggc gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt
    16681 cggtaatgcc gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg
    16741 cgttggcccg gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc
    16801 gccaaaaagc aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg
    16861 cccggattat gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat
    16921 gggctacggg caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga
    16981 ggtttacttc ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct
    17041 tgaaggcaag attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat
    17101 aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca
    17161 ggctgtggcc acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa
    17221 tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct
    17281 ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga
    17341 aaacctgcaa gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg
    17401 ggaagatatc gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc
    17461 aaaagccgag ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa
    17521 cctgcctgca gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg
    17581 ctggggtacc ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat
    17641 gcactctggc ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat
    17701 gaaagggcag attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac
    17761 cgaacagcat ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta
    17821 cggtacgggt cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga
    17881 agaagcagac accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct
    17941 ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc
    18001 atgcttccgt tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca
    18061 ccagttcgac aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct
    18121 ggaagagatg actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa
    18181 aatcatcctt tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt
    18241 atggatcccg gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt
    18301 ccaggcacgt cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt
    18361 tcataccctg aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa
    18421 ctatcagcag gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg
    18481 actggaatat attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct
    18541 ttttttgtct ccctttgata ccgaacaata attactcctc acttacacgt aatactactt
    18601 tcgagtgaaa atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata
    18661 tagcgagctg ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc
    18721 aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag
    18781 agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca
    18841 ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca
    18901 gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt
    18961 atgcacgtcg tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat
    19021 tacgacggcc tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc
    19081 tacaatccgg accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa
    19141 ttcgagcgca ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg
    19201 atcaaagagt acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc
    19261 accatgaccc gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc
    19321 ggcggttatc ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac
    19381 catacctacg gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg
    19441 gtagtgctgt ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac
    19501 tatcttgaac aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat
    19561 accgacaccg gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc
    19621 gcactggtta acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc
    19681 ctcgataaat attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat
    19741 ggtcattata aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa
    19801 tgggcctcgc aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc
    19861 ggcagtacca aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt
    19921 gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat
    19981 ggagggaaca gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc
    20041 ttggaaaacc cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt
    20101 ggcccggaaa tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg
    20161 atcaaaatga tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac
    20221 cgtacccatg aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc
    20281 cacatgacct catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa
    20341 cagatggact ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat
    20401 caggtgatta aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca
    20461 aaacgtcttg gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg
    20521 catctgtatg cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc
    20581 aagcagggga tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc
    20641 cgtgaagatc cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg
    20701 caggcgctgg ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca
    20761 ctgccgatct acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg
    20821 ctgcagctta caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat
    20881 gtgctgaaag cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc
    20941 ggtatccaca acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag
    21001 gcgaaagtga cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat
    21061 gacccggatg caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt
    21121 ccgtctcctc tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg
    21181 taaggagtaa ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg
    21241 ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt
    21301 ccgccgcatt tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca
    21361 gaacgtgttt gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac
    21421 taaagtctgc ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga
    21481 agatgtatgc attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata
    21541 taacgaaacg aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg
    21601 taaaaagccg atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga
    21661 cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt
    21721 taccaaaccg aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac
    21781 tggctatctg gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc
    21841 tgatgatctt cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg
    21901 cgctgctcaa aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg
    21961 gcttatgggt actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga
    22021 tgcgcgcctt taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca
    22081 gcggttcgat cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc
    22141 tgtcaccggc attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg
    22201 tctggatgat ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct
    22261 ggacgccgat gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg
    22321 tgagtggcgt gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc
    22381 agcaggccgc agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt
    22441 tggccgttgc cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc
    22501 cgctactatc cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat
    22561 tctatggttt gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg
    22621 tttcggcccc ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat
    22681 tacgataatc aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc
    22741 agtcacggtg cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg
    22801 acatgcgatc ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca
    22861 cgacaaagac atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt
    22921 cggttaccac accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa
    22981 aatcttcgtt atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga
    23041 attgtgcttt cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa
    23101 gaatagttgg taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa
    23161 acttatcggg ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca
    23221 aaacggcagc atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc
    23281 gagagtcagt ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac
    23341 gggaaatatg agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt
    23401 gtgttagatc aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg
    23461 aatgagtaat ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga
    23521 caatgttttt ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc
    23581 atgtccacgt atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg
    23641 gcgatcgcgg tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca
    23701 tggcaggtag gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg
    23761 acagggtatg tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt
    23821 tttgctgctg gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg
    23881 cgttttgtcg cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg
    23941 tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac
    24001 gtgggaacgt ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta
    24061 ttgccgtggg ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc
    24121 ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt
    24181 caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat
    24241 ggcctgatgc ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg
    24301 atggcggtac tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat
    24361 aagtttggtc gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt
    24421 gcgatgctta gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt
    24481 acgctatatc cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg
    24541 gcgatgaacc aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt
    24601 agcgctatgc taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta
    24661 tcgtttatct atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct
    24721 cacgtgtaaa tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt
    24781 tcttctatct gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag
    24841 gttataattt tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt
    24901 ttacagatgc ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcagga
//
LOCUS       DAEEDS010000056        24604 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEEDS010000056 DAEEDS010000000
VERSION     DAEEDS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24604
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.056"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..374)
                     /locus_tag="KLH32_002534"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="KLH32_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="KLH32_002535"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="KLH32_002535"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBE0139319.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="KLH32_002536"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="KLH32_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBE0139320.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="KLH32_002537"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="KLH32_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBE0139321.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="KLH32_002538"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="KLH32_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBE0139322.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="KLH32_002539"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="KLH32_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBE0139323.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="KLH32_002540"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="KLH32_002540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBE0139324.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="KLH32_002541"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="KLH32_002541"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBE0139325.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNIGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="KLH32_002542"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="KLH32_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBE0139326.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="KLH32_002543"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="KLH32_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBE0139327.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="KLH32_002544"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="KLH32_002544"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBE0139328.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="KLH32_002545"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="KLH32_002545"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBE0139329.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGITSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="KLH32_002546"
     CDS             10736..11161
                     /locus_tag="KLH32_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBE0139330.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="KLH32_002547"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="KLH32_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBE0139331.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="KLH32_002548"
     CDS             12185..13621
                     /locus_tag="KLH32_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBE0139332.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KLH32_002549"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KLH32_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBE0139333.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KLH32_002550"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KLH32_002550"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBE0139334.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KLH32_002551"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KLH32_002551"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBE0139335.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="KLH32_002552"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="KLH32_002552"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBE0139336.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KLH32_002553"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KLH32_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBE0139337.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="KLH32_002554"
     CDS             complement(20454..20747)
                     /locus_tag="KLH32_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBE0139338.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="KLH32_002555"
     CDS             complement(20744..22033)
                     /locus_tag="KLH32_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0139339.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="KLH32_002556"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="KLH32_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="KLH32_002557"
     CDS             complement(23047..23811)
                     /locus_tag="KLH32_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBE0139340.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24604
                     /locus_tag="KLH32_002558"
     CDS             24127..>24604
                     /locus_tag="KLH32_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBE0139341.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAI"
BASE COUNT         6097 a         5941 c         6462 g         6104 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatatcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaataaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
    24601 attc
//
LOCUS       DAEEDS010000057        24482 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEEDS010000057 DAEEDS010000000
VERSION     DAEEDS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.057"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(84..881)
                     /locus_tag="KLH32_002559"
                     /pseudo
     CDS             complement(84..881)
                     /locus_tag="KLH32_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            864..1973
                     /locus_tag="KLH32_002560"
     CDS             864..1973
                     /locus_tag="KLH32_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBE0139342.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            2034..4072
                     /locus_tag="KLH32_002561"
                     /pseudo
     CDS             2034..4072
                     /locus_tag="KLH32_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            4083..4700
                     /gene="dmsB"
                     /locus_tag="KLH32_002562"
     CDS             4083..4700
                     /gene="dmsB"
                     /locus_tag="KLH32_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE0139343.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            4702..5556
                     /locus_tag="KLH32_002563"
     CDS             4702..5556
                     /locus_tag="KLH32_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBE0139344.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            5599..6213
                     /gene="dmsD"
                     /locus_tag="KLH32_002564"
     CDS             5599..6213
                     /gene="dmsD"
                     /locus_tag="KLH32_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBE0139345.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            6372..7664
                     /gene="clcB"
                     /locus_tag="KLH32_002565"
     CDS             6372..7664
                     /gene="clcB"
                     /locus_tag="KLH32_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBE0139346.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(7617..8312)
                     /gene="bioD"
                     /locus_tag="KLH32_002566"
     CDS             complement(7617..8312)
                     /gene="bioD"
                     /locus_tag="KLH32_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE0139347.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(8437..9657)
                     /gene="mlc"
                     /locus_tag="KLH32_002567"
                     /gene_synonym="dgsA"
     CDS             complement(8437..9657)
                     /gene="mlc"
                     /locus_tag="KLH32_002567"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBE0139348.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(9792..10694)
                     /locus_tag="KLH32_002568"
     CDS             complement(9792..10694)
                     /locus_tag="KLH32_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0139349.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            10801..11610
                     /locus_tag="KLH32_002569"
     CDS             10801..11610
                     /locus_tag="KLH32_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139350.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            11626..11883
                     /locus_tag="KLH32_002570"
     CDS             11626..11883
                     /locus_tag="KLH32_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139351.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            12451..12786
                     /locus_tag="KLH32_002571"
     CDS             12451..12786
                     /locus_tag="KLH32_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBE0139352.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            12879..12962
                     /locus_tag="KLH32_002572"
     CDS             12879..12962
                     /locus_tag="KLH32_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139353.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13062..13883
                     /locus_tag="KLH32_002573"
     CDS             13062..13883
                     /locus_tag="KLH32_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBE0139354.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(13922..14251)
                     /gene="mdtI"
                     /locus_tag="KLH32_002574"
     CDS             complement(13922..14251)
                     /gene="mdtI"
                     /locus_tag="KLH32_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBE0139355.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(14238..14603)
                     /gene="mdtJ"
                     /locus_tag="KLH32_002575"
     CDS             complement(14238..14603)
                     /gene="mdtJ"
                     /locus_tag="KLH32_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBE0139356.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15014..16048
                     /gene="tqsA"
                     /locus_tag="KLH32_002576"
     CDS             15014..16048
                     /gene="tqsA"
                     /locus_tag="KLH32_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBE0139357.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16073..17461)
                     /gene="pntB"
                     /locus_tag="KLH32_002577"
     CDS             complement(16073..17461)
                     /gene="pntB"
                     /locus_tag="KLH32_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBE0139358.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(17472..19004)
                     /gene="pntA"
                     /locus_tag="KLH32_002578"
     CDS             complement(17472..19004)
                     /gene="pntA"
                     /locus_tag="KLH32_002578"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBE0139359.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            19528..20472
                     /locus_tag="KLH32_002579"
     CDS             19528..20472
                     /locus_tag="KLH32_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0139360.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20658..22040
                     /locus_tag="KLH32_002580"
     CDS             20658..22040
                     /locus_tag="KLH32_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0139361.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22077..22799
                     /gene="folM"
                     /locus_tag="KLH32_002581"
     CDS             22077..22799
                     /gene="folM"
                     /locus_tag="KLH32_002581"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBE0139362.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(22796..23131)
                     /locus_tag="KLH32_002582"
     CDS             complement(22796..23131)
                     /locus_tag="KLH32_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBE0139363.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            23260..24003
                     /gene="rstA"
                     /locus_tag="KLH32_002583"
     CDS             23260..24003
                     /gene="rstA"
                     /locus_tag="KLH32_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBE0139364.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            24228..>24482
                     /locus_tag="KLH32_002584"
     CDS             24228..>24482
                     /locus_tag="KLH32_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBE0139365.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLEN"
BASE COUNT         5990 a         6266 c         6019 g         6207 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcacagggac gctgacaagt ccttaaagct ggattgtttc
      121 agtgtctgga ccttctcagc cagcagtgat gtcaccaatt cctgaaaaag tacagatgat
      181 tctcgtgact ctggagtgag taactgacgt tcctgtgtga aaactgctgg tctatccccc
      241 tgctcattct tcagaatcga cgtagagtcg cctatagttg agtaatcagt atggttatta
      301 tcatcagtaa gctggcttac gccaccatca atcagcttaa tcagaccaga ggtatcacca
      361 gtgtcgactc tctgctgtgc tgcggtcagg acttccagcg ctaacttaat gcccctctgc
      421 tggtccacac tcaacgctct tgtcgctgca agttccctga ggttggactt ctcgtcaacc
      481 agtgtcgcca cctctacgcc attccagtag tcatcactgg catcagtcag gaactgctca
      541 gccatggacc gtatgtgcgc tgtaagctcc tgtagtgtga cttcaggatt gtccagtagg
      601 aatgccttag ctgtggctgc gagctctctc tgacgcattg tagcggtcct ccggtctgtg
      661 ctgcgggttg agactgatag aaacgggctg gtagctcctt taagtctgaa gcggacagtg
      721 taacgcccgt tggttctccg gactagctgt ggcgtagcaa ggtccttgat ttgtgtcagc
      781 cttgtgtcag gacttgaggt gtagacttgc tgatacagat agggagaccc agagaaagaa
      841 ctggatggat atgcagtatc gtcatggtta atgtcagtca tatcaggacc ttaagtagcg
      901 ttgagtcaac tacttcaagt ccgtccatcc gtctgacagt agagtccatc tggaactacg
      961 cgggaaatac cctcgttaat cagcattctg acatcaacaa aacgcatgaa attctgcagg
     1021 acgaatcgaa atgcgaaatg attgtggtca tcgaaaactt tatgacctca tcggcaaaat
     1081 atgccgacat tctgctggca gacctgatga ccgttgagca ggaagatatt attcctaacg
     1141 actacgccgg taacatggga tatctcattt tcctccagcc agtcaccagc gaaaaattcg
     1201 aacgtaaacc gatttactgg atcctgagtg aagtcgcgaa acgtcttgga cctgacgtct
     1261 atcaaaagtt cacagaaggt cgcacgcagg aacaatggtt acaacatctg tacgccaaaa
     1321 tgcttgccaa agatccggcg ttaccgtctt acgacgaact gaaaaaaatg ggtatctata
     1381 agcgtaaaga tcccaatggc cattttgtcg cctacaaagc atttcgtgac gaccccgagg
     1441 caaatccact taaaacgcct tccggtaaga ttgaaattta ttccagcaag ctggcggaaa
     1501 ttgcccgtac ctgggaactg gaaaaagatg aagtgataag cccactgcca gtttatgcct
     1561 caacctttga aggctgggac tcccctgaac gtagaacctt cccactgcaa ctgtttggtt
     1621 tccattacaa atcccgtact cactcgacct acggcaacat tgatgtcctg aaggcagcct
     1681 gccgtcagga ggtgtggatc aaccctatag atgcacagaa acgtgggatt gccaacggcg
     1741 atatggtgcg ggtgtttaac catcgtggcg aagttcggct accagccaaa gtaacaccac
     1801 gcattctccc tggagttagc gctatgggcc agggagcctg gcacgagccc aatatgtctg
     1861 gcgataaaat cgaccatggc ggatgtgtga atacgctaac cactctgcgc ccttcaccac
     1921 tggcgaaggg aaacccgcag cacactaatc tggtcgagat cgaaaaaata taacccacta
     1981 caaccataaa ttctggcatg acattttgtt tgaaaagcaa taagtgagta atgatgaaaa
     2041 tccataccac agaggcgctc atgaaggctg aaatcagcca tagaagtctg atgaaaacct
     2101 ccgcacttgg cagtctcgcg ctggcaagca gtgctttcac tctgccattt tcccaaatgg
     2161 tccgggcggc agaggctccg gtagaagaga aaacggtctg gagttcctgc accgttaact
     2221 gcgggagccg ctgtctgcta cgtttgcatg tgaaagatga caccgtgtac tgggtggagt
     2281 ctgatacgac aggtgacgac gtttacggta atcatcaggt tcgggcatgt ttacgcgggc
     2341 gctctatccg ccgacggatg aatcatcctg acaggttgaa atatcccatg aagcgcgtcg
     2401 gcgagcgcgg tgaaggtaaa tttgaacgga taagttggga cgaagccctg gataccatca
     2461 gtgataatct tcggcggatc ctgaaagatt acggcaatga ggctgtacat gtcctgtacg
     2521 gaacaggcgt agatggcgga aatatcacca actcgaacgt cccgtaccgt ctgatgaact
     2581 cttgcggggg ttttctcagt cgctatggca gctacagtac cgcacagatc agtgccgcaa
     2641 tgagttatat gttcggtgcc aatgatggca acagcccgga tgatatcgcc aatacgaaac
     2701 tggtcgttat gttcggaaat cacccggcag aaacgcggat gagcggcggt ggtgtcactt
     2761 actacgtcga gcaagcccgc gaacgttcaa acgcacgcat gatcgtcatc gatccacgct
     2821 ataacgacac agctgccggg cgtgaagatg aatggctgcc cattcgccct ggcaccgatg
     2881 gcgcactggc ctgtgcgatt gcctgggtac tgattactga aaacatggtc gatcagccat
     2941 ttctcgacaa atattgtgtt ggttacgatg aaaaaacgct gcccgccaac gcaccacgta
     3001 acgcgcatta taaagcctat attctgggcg aagggcctga cggcatagct aaaacgccgg
     3061 aatgggcagc aaaaatcacc agcatcccgg cagaaaaaat tatccagttg gcacgagaga
     3121 tcggttcagc aaaacctgct tatatttgtc agggttgggg gccacaacga cactccaacg
     3181 gcgaacaaac gtcccgcgct attgccatgc tttccgttct taccggtaac gtcggcataa
     3241 acggcggcaa ctcaggtgta cgcgaaggta gctgggatct gggggtagaa tggttcccga
     3301 tgctcgagaa tcctgttaaa acgcagattt ccgtttttac atggacagat gccatcgacc
     3361 atggtacgaa aatgaccgcg acccgtgatg gtgttcgtgg aaaagaaaaa ctggatgtcc
     3421 ccatcaagtt tttatggtgc tacgccagta acacattgat caatcaacat ggcgacatca
     3481 atcacaccca tgaggtgctt caggatgaca gcaagtgcga gatgattgtt ggcattgacc
     3541 acttcatgac ggcctcggct aagtattgcg atatcctgtt gcctgacctg atgccaacag
     3601 agcaagaaga ccttatctct catgaatctg cagggaatat gggctatgtg atcctcgccc
     3661 aacccgcaac ctcagcaaaa tttgaacgca aacccatcta ctggatgctg agtgaagtcg
     3721 ccaaaccaaa gcgcgtaccc actccagcta cggcaatatt gatgtgttac agcaggcctg
     3781 cccacaagag gtgtggatca accccattga tgctcaggca cgcggtatcc gtcatggcga
     3841 taccgtgcgg gtatttaaca ataatggaga aatgctgatt gccgcaaaag tgactccgcg
     3901 tattctgcct ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg atatgtttgg
     3961 tgaccgggtc gatcacggcg gcagtatcaa tattctgacc tctcaccgcc cttcaccgct
     4021 ggcaaaggga aacccgtcgc acagcaatct tgtccagatc gaaaaggttt aaggagtagc
     4081 ccatgaccac acaatatgga ttttttatcg attccagccg ctgtactggc tgtaaaacct
     4141 gcgaactggc gtgcaaagat tttaaagatc ttggcccgga agtcagtttc cgccgtattt
     4201 atgaatatgc tggcggcgac tggcaggaag ataatggcat ctggcaccag aacgtgttcg
     4261 cttattatct ctccatctcc tgtaaccatt gcgatgaccc cgcatgtaca aaagtctgcc
     4321 ccagcggcgc aatgcataag cgtgaagatg gctttgtggt ggttgatgaa gatgtttgta
     4381 tcggctgccg ctactgccac atggcttgtc cgtacggcgc accacagtac aatgctgaaa
     4441 aagggcacat gacgaagtgc gatggttgtt attcgcgcgt cgccgagggg aaacaaccca
     4501 tatgcgtcga atcctgcccg ctgcgtgcac ttgagttcgg tccaattgaa gagctgcgcc
     4561 agaagcacgg cactctggct gccgtcgcgc cactgccgcg cgcgcatttc acaaaaccca
     4621 atatcgttat caaacctaac gccaacagcc gcccgaccgg cgataccact ggttatctgg
     4681 ctaatccgga ggaagtgtaa aatgggaaat ggatggcatg aatggccact ggttatcttt
     4741 accgtattgg gtcaatgcgt agtaggcgcg ctgattgtta gcggaattgg ctggttcgcc
     4801 gccaaaaatg acgcagaccg tcagcacatt gttcgtggca tgtttttcct ctggctgttg
     4861 atgggcatcg ggtttattgc ctctgtcatg cacctgggat cgccactgcg agcgtttaac
     4921 tcgctgaatc gaatcggagc ttctggtctg agtaacgaga ttgctgccgg ttccatattt
     4981 tttgccgttg gcggactatg gtggctggtg gcggtcatcg gtaaaatgcc gcaagcgttg
     5041 gggaaactct ggttacttgt cagcatggcg ctcggcgtca ttttcgtctg gatgatgacc
     5101 tgcgtgtatc aaatcgacac cgtgccaacc tggcataacg gttacactac gctggctttc
     5161 ttcctgacgg tattgttgag cggtccaata cttgctgccg caattctgcg ggcagcccgc
     5221 gttacattta ataccacacc atttgccatc attagcgttc tggcgctgat cgcttgtgcc
     5281 gaggtgattg tgctgcaagg tttgtctctg gcgtctattc actcatccgt gcaacaagcc
     5341 agcgcactgg taccagatta cgcctcctta caggtctggc gtgtggtatt gttatgcgca
     5401 ggtcttggat gctggctttg tccgctcatt cgtcgccgtg aacctcacgt tgccggactt
     5461 gttctggggc taatcctcat acttgttggt gagatgatcg ggcgtgtgct cttttatggc
     5521 ctgcacatga cagtaggaat ggctattgca ggttaacaaa ttggtgcggg gcaacccgca
     5581 catttcagga tgttaggaat gacccatttt tcacagcaag ataatttttc tgtcgcggcg
     5641 cgcgtgttgg gcgcgctgtt ttattacgct ccagagagtg cggaagccgc acctctggtt
     5701 gcggtactca ccagtgacgg ctgggaaact cagtggcctc taccagaagc gtcattagcg
     5761 ccgctggtga cggcttttca gacacagtgt gaagagactc acgcccaggc ctggcagcgt
     5821 ttgtttgtcg gcccgtgggc actgccgtct ccgccgtggg ggtcggtctg gctggatcgc
     5881 gaatctgtgc tgtttggcga ttcaacattg gcacttcgtc agtggatgcg cgagaaaggc
     5941 attcagtttg aaatgaagca aaacgaaccg gaagatcatt ttggttcact gctgttgatg
     6001 gctgcgtggt tggcggagaa tggtcgccag acagaatgcg aagaattgct ggcatggcac
     6061 ctttttccgt ggtcaacacg ttttcttgat gtttttatcg aaaaagcaga acaccctttc
     6121 taccgtgcac tgggtgaact ggctcgctta acgctggcgc aatggcaatc acaactgttg
     6181 attcctgtcg cggttaaacc actctttcga taggatcacc gtaatattgc cggatggtga
     6241 cgattacgcg cgattcggca ataatacgtt acaatgtcac gtttttttat ctcttaaagc
     6301 acgcactgct tttgcggttg gcctcttttg ccgcaaaata gtcgcccgtg tttcattgcc
     6361 catttctgct catgcatcat ctacacatct atccggatct gcgcactatg ttccgccgtc
     6421 tgcttatcgc aacagtcgtc ggtattctcg ctgtctttgc cgttgccggg tttcgtcatg
     6481 cgatgctgct actggagtgg ttgttcctca ataatgactc cggcagtctg gtcaatgcag
     6541 cgacaaacct ttcctcctgg cgacggctgc taactccggc gctcggcgga ctggcggcgg
     6601 gtttgttgct gatgggctgg cagaaattta cccaacaacg ccctcatgcg ccgaccgatt
     6661 acatggaagc gttgcaaacc gatggacagt tcgattacgc agcaagcctg gttaaatcgc
     6721 ttgcctcgct gctggtagta accagcggca gtgcaattgg tcgcgaaggt gcgatgattc
     6781 ttttagctgc ccttgccgcc tcctgttttg cccaacgttt tacgccacgc caggaatgga
     6841 aattatggat cgcctgtggg gccgcggcgg gaatggctgc ggcctatcgt gccccgcttg
     6901 ctggcagttt atttatagcc gaagtgctgt ttggcactat gatgttggcc tctctcggcc
     6961 cggtgattat ttccgccatc gtggcatggc tggttagcaa tctgattaat catagcgacg
     7021 cgttactcta caacgtacaa ctctcagtga cggttcaggc tcgtgactat gcgctgatta
     7081 tcagtacagg tgtgctggca ggtctgtgcg gaccactgtt gttaacgtta atgaacgcct
     7141 gtcatcgtgg atttgtgagt ctcaaacttg cgccgccctg gcaactggca ctagtcgggt
     7201 tgatcgtggg tctgctttcc ctgttcacac ctgcagtgtg gggcaacggc tatagcaccg
     7261 tacaatcctt tttaaccgcc ccaccactgt taatgatcat tgccgggatc ttcctttgta
     7321 aactgtgtgc cgtgctggcg agtagcggtt ccggcgcacc cggtggtgtc tttacaccga
     7381 cgctatttat cggtcttgcc attggcatgt tgtatggtcg tagcctggga ttatggttcc
     7441 ctgatggcga agaaattacg cttttactcg gattgaccgg gatggcgaca ctgttggcgg
     7501 caaccacgca cgcgccgatt atgtcgacgt tgatgatatg tgaaatgacc ggggagtatc
     7561 agctactccc cggtttattg attgcctgcg taattgcgtc ggtaatttcg cggacgttac
     7621 accgtgactc tatctaccgc cagcacactg cgcagcatag ctaagcggat gtattgcccc
     7681 agttcacgct gttcggcgcg cggcagataa ggcagttcac caatgagcgg tgccggaagt
     7741 tttttaccca gcacatcaat gatttccgca taatgcgcca gtcctgggtt gattcggtta
     7801 gccacccagc caatgagcgg cagcccgtcg ttggcgatcg cctgagctgt tagcagtgca
     7861 tggttaatgc aaccttcctg aataccgaca accatcaaca ccggcagttg ttcctgcact
     7921 acccattcag agagtggacg caaatcattc atcagactgc gccagccgcc agtcccttct
     7981 accacgacat gatcgacttt atcggtcagg tttgccaggc cgtttgaaat gagggtgtaa
     8041 ttgattgggc aactgtgcgc cacgctactt tcttcttcgc ttaacgcgat aggattaact
     8101 gcttcataag gcagttcgat ggttgaaaca ctctgcaaca ccaaggcatc tttattacgc
     8161 agcccttcgg gtgtctcttt gctccccttc gctacgggtt tatatcccgc aacccttttt
     8221 ccctgggagg ctaacgcttg tagcaatgcg cgggaaacca ccgttttccc tacagaagtg
     8281 tctgtaccgg taataaagaa acgcttcagc atcactaact ccaccgttat gcttcacaaa
     8341 tataaactag gaaaataatt aaccttgaaa gtctaagtta tgctttcctg gcccaaattg
     8401 agatagcgca aattttggta gaacagttaa aaaatgttaa ccctgcaaca gacgaatcaa
     8461 caaagaaccg ttatacatcg cgtcttttac cagtgcagcg cctgccatcg tgccctggtt
     8521 agaaaactga gtactctcaa cgctaatgtg ctgactatac gcaggaaggg cctgctgacg
     8581 gatgctgtct gagatgaccg ggaagaggat atctgccgct ttacttaacg gtgagccaat
     8641 cagtattttt tgtgggttaa ataaattcac catgatggca agaatgcgcc ctacatgcgc
     8701 gcccaccccg ataatgatgt cttttgccag tagatcgccg cgcaatgccg cctgacacaa
     8761 tgagtccacg gttaacggtt gcccatgtaa catcgagctc atggattgat tgagacgcag
     8821 ctgtgccagc tcaagaatac tgtccacact ggcgatggtt tcgaggcagc cgtggttccc
     8881 gcaataacag cgtttaccat atgggtcgac ctgtgtgtgg cctatttcca cgagactact
     8941 gctgcctgcg tgtagcagat gaccatcggt aatgacgccc acccccacgt tgtgatcgat
     9001 aaccacctga atcacatcgc gcgccccgcg tgaggcacca aacaaggcct ctgccatcgt
     9061 ccatgcgctg atatcatgct gaatataaac cggtacgccg gtatgctgct ccagcgcctc
     9121 gccgagcggc atctctttta catcctcgta gaacggcatg cgatgtacaa taccattttc
     9181 cgtatcaata attcccggca gggttatggc aatcgaagtt agacgctcaa gttttttctg
     9241 gtggcggata aaaaactgat cgatatggga aatgatacga tccagcaatg gcaagtcatc
     9301 ttttaacgcc agttcctgcg actcttccac caccagtttg ctgctcagat cgcgcagagc
     9361 aaggaaaatc tccccgcgac taatgcgcag agaaagatag tgccaggctt cagtttcaac
     9421 caccagcccc accgccggac ggccacggtt ccccgcttct ttgatttcca gctctcgcac
     9481 caggtgtgct tcgagcatct cacggacaat tttagtgata ctggcaggag ccagctgcgc
     9541 cagacgggaa agatcgatac gcgagactgg accaagctga tcaatcaggc gataaaccgc
     9601 gcccgcgttg gtctgcttta tttgatcaat gtgcccaggc tggttttcag caaccaccgc
     9661 atactcccta tattttcgcg ctccgaaata atctgtaggc tatggtgaag cacttcaata
     9721 cgtgtcgtca aatttttact taggcatgtg attaacagca catttttcgg gctttttcgc
     9781 tgaaatttcc ctcacctgag agcattcagc agatgaatac gaaagttacg cgcagctgga
     9841 ctttgttcat gatgtttcgg ccagaccaac cacatttcag aaaccgcatc ctcttcagca
     9901 atcggcaccc agcgcatttc gttgagctga acacgtttaa atgacgcagg caaaattgaa
     9961 acacccagac cggcggaaac cagaccgatg atggtcattg cctcgcccac ctcctgagtg
    10021 atgacgggcg tcaaatggta acgtcgcatc agtccgagaa tatcgtcata cagcccagtc
    10081 ccgacgtgcg gatcaaaaaa gacaaagggt tctttcgcca gttcagccag ttcagccagt
    10141 gttacactcg gattatttgc caggggatga tcgtacggga tcatcgccat aagcggttca
    10201 tggacgatga ctgcgtgttc aagcgtctcc ggtaacgctg tgttacgcag caatcccata
    10261 tccagcgttc cttcaatgag cggagcgatt tgctcgcgag tgttcatttc gcgggtttgt
    10321 aaatggacat caggataatc acggcgaaac agcgataacg tatcggacac cgcccgaata
    10381 aaaggagcgg acgaagtaaa accaatacgc aactcccccg cttcaccctg atgcagtctt
    10441 tcagcgcgag cggcagcgtc atccaccata gacaggattt gccgactatc tgcaagaaac
    10501 tgttttcctg ctgccgtcag caatacactg cgattagttc gtgccagcag tcgggcaccg
    10561 atttgttgct ccagtgcctg aatctgctga cttagcggcg gttgcgaaat attcaggcgg
    10621 gcagccgcgc gcccaaaatg cagctcttcc gcaacagcaa caaagtaacg cagatgacga
    10681 agttcaatat tcatatttaa aacatcttat ttgagattat taatatatta gacagaacaa
    10741 ttcgattttc ctaccctatg tataagcctg atccacaggc atatttagca aggatttcaa
    10801 gtgagccgta ctacaactgt tgatggcgct ccggcaagcg acactgacaa gcaaagcatt
    10861 tctcagccaa atcaatttat taaacgcggt acgccgcaat ttatgcgcgt caccctggcg
    10921 ctgttctctg ccggactggc aacctttgca cttctctatt gtgtgcagcc tatccttccg
    10981 gtgctttcgc aggagtttgg cttaacgccc gcgaacagta gtatttcact gtccatttcc
    11041 acggcgatgt tggctatcgg tttgctattt actggcccgc tatccgatgc cattggtcgc
    11101 aaaccagtga tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg
    11161 atgaccagct ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc
    11221 gtggcagctg ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt
    11281 tcgatggggt tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagt
    11341 ggtgtcttca cggacttttt caactggcga attgctctgg cggcaatcgg ttgtttcgcg
    11401 ctggcctcgg cgttgatgtt ctggaaaatc ctccctgaat cacgccattt ttgcccgact
    11461 tcgctgcgcc ctaagacgtt atttatcaac tttcgtctgc actggcgtga ccggggatta
    11521 ccgttattgt tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac
    11581 atcggctatc ggttgatgct ttccccctag catgtcagtc aggccgtggt tggcttatta
    11641 tcgctggctt atttgaccgg tacatggagc tcacccaaag ccggaaccat gaccacccgc
    11701 tatgggcgcg gtccggtgat gttgttttcg acgggagtta tgccgtttgg tttactgatg
    11761 accttattta gctcgctgtg gctgatcttt gccggaatgt tactcttctc agccggattc
    11821 ttcgcagccc actcagtagc cagcagctgg atcggccccc gcgcaaaacg cgctaaaggc
    11881 taggcctcct cgctgtatct gttcagttac tatctggggt cgagtattgc cgggacgctg
    11941 ggtggtgttt tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg
    12001 ctggtcattg ctctgctggt cgggacgcgt ttgcatcgtc gtctgcacgc ctgaaaaata
    12061 agtccggact gcggtaaata cccgtccgga cttattgcct gctcaaacca acgttaatag
    12121 ccatcctaaa atagacgaag cgccagccaa ttcccgcagc gcatctagcg tcatcaggat
    12181 tataagtacc caaataaacg gattcatttt gctgtgtgtc atttattact gatgcgcagt
    12241 tattctaccg ctttgtaagt agtataatag ttaacccgat caagactact attattggta
    12301 gctaaatttt ccttaagtca caatacgtta ttatcaacgc tgtaatttat tcagcgtttg
    12361 tacatatcgt tacacgctga aaccaaccac tcacggaagt ctgccattcc cagggatata
    12421 gttatttcaa cggccccgca gtggggttaa atgaaaaaac aaattgaggg tatgacaatg
    12481 aaaaaagtat tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct
    12541 gcagagactg cgaccacacc tgctccgact gcgacgacca ccaaagcagc gccagcgaaa
    12601 actacacatc ataaaaaaca gcataaagca gcacctgccc agaaagcgca ggcggctaaa
    12661 aagcatcata aaaatacgaa agctgaacag aaagcccctg aacaaaaagc gcaggcagcg
    12721 aagaaacacg ccgggaaaca cggccatcag caaccggcaa aacctgctgc acaacccgca
    12781 gcgtaagttt tcaacagtaa tgctggcgcg ccccctcgcg cctgaaaatt acggtgctaa
    12841 gcgggtaacg tttagcaccg cctttagcgg agggtagtat ggtgggccgt tatcgctttg
    12901 agttcattct tatcatcctt attttatgcg cactgattgc cgcccgtttt tatctttcct
    12961 gactgtagtt atctgatttt actcccactt tcatcccgtc ccgtctatag tatttatgag
    13021 ggtttgcttt taataatcat aattacccac cagagtgtga tatgcgtaca accattgctg
    13081 tagtgttggg tgcaattagt ttgacgtctg cttttgtgtt tgcagataaa ccagacgttg
    13141 ccaaatcggc aaacgatgag gtcagcaccc tgttttttgg tcatgatgat cgtgtgccag
    13201 tgaatgacac gacccaatca ccgtgggatg cggttgggca actggaaacg gccagcggca
    13261 atttatgtac ggcgacgctg attgcaccca atctggcatt aacggcagga cactgtttat
    13321 tgacgccgcc taagggtaaa gcggataagg cggtagcgct gcgttttgtg tcaaataaag
    13381 gtctttggcg ctatgagatc cacgacatag aaggccgcgt tgatccgaca ctgggaaagc
    13441 ggttaaaagc agatggggat ggttggattg ttcctcccgc agctgcaccg tgggacttcg
    13501 gattgattgt gctacgtaat cccccttctg gcattacacc gttgccgtta tttgagggag
    13561 ataaagccgc gcttactgcc gcattaaaat cggctggtcg taaagtgact caggcgggat
    13621 accctgaaga tcatctcgat acgttataca gtcatcaaaa ctgtgaagtg actggctggg
    13681 cgcaaacgtc ggtgatgtca catcagtgcg ataccttgcc gggtgacagc ggttcgcctc
    13741 tgatgttgca taccgatgac ggctggcaat taattggggt gcaaagttcg gctcctgccg
    13801 cgaaagatcg ctggcgcgcc gataaccggg ccatttctgt taccggtttt cgcgacaagc
    13861 tggatcaact gtcgcaaaaa taatgttcaa acgctgcccg acagcgcggg cagcgtcttc
    13921 atcaggcaag tttcaccatg atcattccag ccaacagcaa gaccaggcca atccagcctt
    13981 tacgatttaa ccgttgacca aacaagatcc aacctgcggc taacgtggcg gcaataccaa
    14041 acccgcccca caatgcataa acgacagaca agtcgatccc tttaacggcc tgagaaagcg
    14101 cactaaaggc cgccagcacc gccgccaggg agagcaagcc aaatattttg cgacgaaagc
    14161 cgtcagaaaa tttcaaaaag acgttagcaa cgatttccag cacgattgcc aatgccagcc
    14221 aggcggcgtg aacccattca aactgcgcca tggttcacct ccagttcagg tttacgcgct
    14281 ttacgggtac ctgatttgat caacactatc ccggcgacca gggtggttaa cccggcaatt
    14341 ttcatcagcg ataaactttc gtcgaataac aaaacgctaa acaaggtaat aaataaaata
    14401 ccgatacctt cccacagcgc ataagctacg cctaaggcga tttttttaac ggcgaaagag
    14461 agaaatatat acgacagaga aatcatcacc agcattaaaa taaagccgcc atttccctca
    14521 ctgacgctcg cccatttcat tgacagcgta ccggtaattt ctgtagcaat agccagacct
    14581 aataaaatcc aataaatata cattgtcctt ctcctgcaag agaactattt taattttcgc
    14641 ttaattcagc gaaaccaaag taaagtggtc aagccctgcg catagggcaa gcttaggcag
    14701 aagaaaggac taaagcgcgt tgcgccagtg ctgctcacct acgagcaaga tagtggatga
    14761 ggtacgaaga tgggtaaatg tagaaaataa cgtcctgaac aaattgtcca taatattaca
    14821 attatccgca gtgttgcttc tcgtcatcgc agatgataat tgtcctcggt agttgaacac
    14881 gcctgatttg tatcatagct taagaattaa ctcaaaatat tttcacttct ttacctgagc
    14941 ggtttgattt tcgttatgat gacggagcga aaaagacatt attattagca aaggaagaaa
    15001 aacggggaca agcatggcaa agccgatcat cacgctcaat ggcctaaaaa tcgtcattat
    15061 gttgggaatg ctggtcatta ttctctgcgg tatccgtttt gccgccgaga tcatcgtgcc
    15121 gtttattctc gcattattta ttgctgttat tcttaacccg ctggtgcaac atatggtccg
    15181 ctggcgtgtg ccacgtgtac tggcagtgtc gattttgatg acaatcatcg tgatggcgat
    15241 ggtgttgcta ttagcttatc tgggttccgc gctcaacgag ttgacgtgga cgttaccgca
    15301 atatcgcaac tctattatga cgccgctgca agctcttgag ccgttgttgc aacgcgtagg
    15361 gattgacgtc tcagttgacc aactggcgca ttacattgat ccgaacgcgg cgatgacgtt
    15421 gctcaccaac ttattgacgc agttatctaa tgccatgtca tcaatatttt tattgctgct
    15481 gacggtgctg tttatgctgc tcgaagtgcc acaattgccc ggaaaatttc agcaaatgat
    15541 ggcgcgtccg gttgaaggga tggcggcgat tcaacgtgcg attgacagtg tttctcatta
    15601 tctggtgctg aaaacagcca tcagcatcat caccggcctg gtcgcctggg cgatgctcgc
    15661 cgcactcgat gttcgcttcg cttttgtctg gggattgctg gcctttgcgc ttaattacat
    15721 ccctaatatt ggttcagttc tcgcggcaat cccccctatc gctcaggtac tggtgtttaa
    15781 tggcttctac gaagcgttgc tggtgctggc gggatatctg ctgattaatc tggtcttcgg
    15841 caatattctg gagccgcgca tcatgggacg tgggctgggg ctttcgacat tggtggtatt
    15901 tttgtcgttg attttttggg gatggttgtt aggaccggtg ggtatgctgc tttccgtgcc
    15961 gttgacaatt attgtcaaaa ttgcgcttga acaaacagcg ggaggtcaaa gcatcgccgt
    16021 tctgttaagc gatctcaata aagagtgacg gcctccgcag aggccgtcag ggttacagag
    16081 ctttcaggat tgcatccacg ctggctttgg cgtcaccaaa cagcatgtgg gtgttttcct
    16141 tgaagaacag cgggttttgc acaccagcat agccagtgtt catcgaacgt ttaaagacaa
    16201 tcacgttctg cgctttccac acttccagca caggcatacc agcaatcgga ctcttcggat
    16261 catcctgcgc cgccgggtta accgtatcgt tagcaccaat caccagtacg gtatcggtat
    16321 cagcaaagtc atcgttgatc tcgtccattt ccagcacgat gtcatacggt acttttgctt
    16381 cagccagcaa tacgttcata tgtccaggca aacgccccgc aaccgggtgg ataccgaaac
    16441 gcacattaat accacgagcg cggagtttct cggtaatttc agcgacagga tattgcgcct
    16501 gcgcgactgc catgccgtac cccggagtaa tgatcactga atgggagttt ttcagcagtt
    16561 ccgctgtctc ttctgcggtg atttcgcggt gctcacccac ttcctgatca tcgccagtag
    16621 aagagccgtc ggtgccgaaa ccacccgcaa taacgctgat aaaggaacgg ttcatcgcct
    16681 tacacataat gtaagaaagg atagcccccg aagaaccgac cagcgcaccg gtcacaatca
    16741 gcaggtcgtt gctgagcata aagcccgcag ccgcagccgc ccagccggag tacgagttca
    16801 gcatcgacac caccaccggc atatctgcac caccgatgga ggcgactaaa tgccagccga
    16861 ataccagcgc aatggcggtc attatcagca atgccagcac ttgcaggccg acgctgtccg
    16921 tgcgaacaaa tacaatcagc agcaagaagg aaacgaccag agccgccagg ttcattttgt
    16981 gacggtttgg cagcatcaac ggtttagacg aaatcttgcc acacagtttg ccgaacgcca
    17041 ccaccgagcc cgtgaacgtt accgccccga tgaagatacc gaggaacact tccgtcaggt
    17101 gaatattgac cagaatcggt gccattcccg cgtcatgatg cagataactg ttaaagccaa
    17161 ccagcactgc cgccagaccc acgaagctgt gcaggatcgc caccagttct ggcatttcgg
    17221 tcatttcaac tttcttcgcc agccggatac caattgcccc accaatgacc atcgccagca
    17281 agatccagcc aacattaccc gtatccggtc caaaaatggt tgcgattaac gcaatcgcca
    17341 tcccggcgat accgaagttg ttaccctggc gagacgtttc atgtttcgaa agaccggcca
    17401 gactgaagat aaacaggatc gcggcaacaa tgtatgcagc tgtaactaat cctccagaca
    17461 tatgttaccc cttaattttt gcggaacatt ttcagcatgc gctgagtcac ggtgaagcca
    17521 ccgaaaatat taatgctggc tataagcacc gcgataaaac taaggaagct aacccagcct
    17581 ccctggccaa tctgcaacag tgctccgaca acaataatcc ctgaaatcgc gttggtaacc
    17641 gacatcaacg gtgtatgcag cgcgtgcgat acattccaca ccacgtaata accgacaacg
    17701 caggccagcg cgaaaacggt gaagtgccca agaaactctt tcggcgcaac gcttgacagc
    17761 caaccgaaca ggatgattgc cagcgccatc aacgcgtatt tacgccacgg tgagcaggca
    17821 catttttcct cagttttcac ttccggtgcc gctttttgtg ccgcctgcgg ctgagctgat
    17881 acctgaatcg gcggtgccgg ccaggtaatt tcgcccgcac ggatcacggt cacgccgcga
    17941 atcaccacat catcaaaatc aacagtgata ttgccgtctt tctctttgca caacagtttc
    18001 agcagattaa cgaggttagt accgtaaagc tgtgaggatt gcgtcggcag acggcccgga
    18061 agatcggtat aaccaatcac tttgacacca ttttccgtac tgaagatttc acccggcacg
    18121 atgtattcac agttgccacc gttttgggct gccaggtcga caatcacact gcccgccttc
    18181 atggagtcaa ccatttcacg ggtaattagc ttcggcgctg gtttgcctgg aataagcgcg
    18241 gtggtgacaa tgatatcgac ctcttttgcc tgggcggcaa agagttccat ttccgctttg
    18301 atgaacgcgt ccgacatcac tttggcatag ccatcgccgc tgcccgcttc ctctttaaaa
    18361 tccagctcga ggaattccgc gcccatactt tgaacttgtt ctttcacttc cgggcgggtg
    18421 tcgaatgcac gcacaatcgc gccgagactg tttgctgcgc caatggcggc caaacctgca
    18481 acaccggcac caatcaccat cacttttgcc ggtggcactt tcccggccgc agtgatttgc
    18541 ccggtaaaga agcgcccaaa ttcatgtgcc gcttcaacaa tggcgcgata accggcgata
    18601 ttcgccatcg agcttagtgc gtccagcgat tgtgcgcgtg agatacgcgg cacagagtcc
    18661 atcgccatca cggtcacgtt acgttccgca agtttttgca ttaattccgg attctgcgca
    18721 ggccagataa aactcaccag cgttgtcccg ggattcagta acgcaatttc atcatctaac
    18781 ggcgcattga ccttcagaat gatctctgac tgccagacgc tattcccttc tacaatttca
    18841 gcgcccgctt gcacaaacgc tttatcgtca aaacttgcca gttgacccgc gccgctctct
    18901 accgcgacgg taaaacccag tttcagcagc tgttccactg tttttggcgt tgctgcaaca
    18961 cgggtttcat tggttaaccg ttctcttggt atgccaattc gcatgatatt cccttccatc
    19021 ggttttattg atgatggttt gcctgtgtca ggagccacac aagctgctca tgtacgagct
    19081 aaatgttact ccgttaaaat aaattagtaa caaacgccct ataacgtact gaaaattatg
    19141 cctgtgatct agcgccaaaa atcagtattt cggcgtgaac tcgcaaaata ttaacgattc
    19201 agccgtgata gtgggataaa caccttagaa cgccggataa agactgataa ttgtcttcga
    19261 cggtcgggta aaacgagaca tcgccccggc acgaatcact acttaacatt aaattaactt
    19321 atacaattca gttgcttcag tagtaatgat gctgatacgg ctgtttttta agcatagacg
    19381 gtcatttgag caggattaaa attggtttaa ggaatgtgat atgaaaaatg acgcagacag
    19441 ttacaccgtt taaatgcaat aatcagccac gtttctcgtt aataacaata ccagtacctg
    19501 gtttgcgcaa ggcgaaggat tatttttatg aagcttaaga acaccctcct ggcgtcggca
    19561 ctgctttctg ctacggcatt ctccgttaac gcagcaacag aactgacacc ggagcaagcg
    19621 gcagcggtta aaccttttga ccgtgtagtg gttaccggtc gttttaatgc tattggcgaa
    19681 gcggtgaaag ccgtttctcg tcgcgcagat aaagaaggtg ccgcctcttt ttatgttgtc
    19741 gacacttctg attttggtaa cagcggtaac tggcgtgtgg tcgctgacct ctataaagcc
    19801 gatgctgaaa aagcagaaga aacaagtaat cgcgtaatta acggtgttgt cgaactgccg
    19861 aaagatcagg ctgttctgat tgaaccgttt gacacggtca ccgtccaggg cttctatcgt
    19921 agccagccag aagtcaatga tgccatcacc aaagcggcaa aagcgaaagg tgcctactct
    19981 ttctacatcg ttcgtcaaat cgatgccaac cagggcggca accagcgtat tactgcattc
    20041 atctataaaa aagatgctaa gaaacgtatc gtccagagcc cggatgtgat cccggcagat
    20101 tccgaagcag gacgtgcagc tctggctgcc ggtggcgaag ccgcgaagaa agttgagatc
    20161 ccgggtgttg cgactaccgc atcaccaagt tctgaagtcg gtcgcttctt tgaaacccag
    20221 tcatcaaaag gcgggcgtta caccgtcacg ctcccggatg gcactaaagt cgaagaactg
    20281 aacaaagcga cagcagcgat gatggtcccg ttcgacagca tcaaattctc tggcaactac
    20341 ggcaacatga ccgaagtctc ctatcaggtt gcgaaacgtg ccgcgaagaa aggtgctaag
    20401 tattaccaca tcacccgcca gtggcagaaa cgtggtaata acctgaccgt cagcgcagat
    20461 ctgtataaat aactattcag ttactcctat aaggcggctt gatgccgcct ttcgcatttt
    20521 tagtcacaaa tttccccgcg accattgcat gcagacacat ctcttcgtaa aatcccgcgc
    20581 ttcagtggca aactccattt ttatgcgttt tgccctaata tttattcttc ttatcacgtt
    20641 ttaatcactg gatatcgatg gaaaagaaac tgggactgag cgcactcacc gcgctggtat
    20701 taagctcaat gctgggcgcg ggtgttttca gtctgccgca aaatatggcg gcagttgcca
    20761 gcccggcagc actgctcatc ggctggggta ttactggcgc tggcatttta ttgctggcct
    20821 ttgccatgct gatcctcacg cgcattcgtc ccgaacttga tggcggtatc tttacctatg
    20881 cccgcgaagg gtttggcgag ctaatcggct tttgttccgc atggggatac tggctgtgcg
    20941 cggtcatcgc caacgtctcc tatctggtga tcgttttttc cgcgttaagc ttttttacgg
    21001 acacgccgga attgcgcctg tttggcgatg gtaatacctg gcaatcgata gtcggtgcat
    21061 cggctttatt gtggattgtt cactttttga tcctgcgcgg ggtgcaaacc gctgccagca
    21121 ttaacctggt ggcgacactg gcaaaattgt tgccgctggg tctgtttgtt gtgctggcga
    21181 tgatgatgtt caaactggat accttcaagc tcgacttcac cggacttgca cttggcgtac
    21241 ctgtttggga acaggtgaaa aacaccatgc tgatcaccct gtgggttttc attggtgtgg
    21301 aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg tgatgttggc aaagcgacac
    21361 tgctggcggt tctctccgct ctgggcgttt acttgctggt aacgctgctt tcgctgggtg
    21421 tggtggcacg tcctgaactg gctgaaattc gtaacccgtc aatggccggt ctgatggtgg
    21481 aaatgatggg accatggggc gaaatcatca tcgctgccgg tttgatcgtt tccgtttgcg
    21541 gcgcgtactt aagctggacc atcatggcgg cagaagttcc gttcctggct gccactcata
    21601 aagcattccc acgcattttt gcgcgccaga acgcacaagc tgcgccatcc gcctcactgt
    21661 ggttgaccaa catctgcgtg caaatttgtc tggtactcat ctggctgacc ggttccgatt
    21721 acaacacgtt gctgaccatc gcctcagaaa tgattctggt gccctatttc ctggtcggcg
    21781 cgttcttgct gaaaatcgca acccgtccac tgcataaagc ggtaggtgtc ggtgcctgca
    21841 tttatggctt atggttattg tacgcctctg gtccaatgca cttgctgtta tccgttgttc
    21901 tgtatgcacc ggggctgctg gttttcctgt atgcgcgcaa aacgcataca catgataatg
    21961 tactgaaccg tcaggaaatg gtgctgattg gcatgctgtt aattgcctca gttccggcga
    22021 cctggatgct ggtgggataa cgttcatccc atcgttggat agcaaaggag ataacgatgg
    22081 gtaaaaccca gcccttgcca atattaatta ctggcggagg tcgtcgcatc ggcctcgccc
    22141 tcgcatggca tttcattaat caaaagcaac cggtgattgt cagctatcgg acacactatc
    22201 cagccattga tggactgatt aatgcaggtg cgcagtgtat tcaggctgat ttttcgacca
    22261 acgacggtgt gatggcgttt gccgatgaag tactaaaaac cacccatggt ctgcgtgcta
    22321 ttttgcataa cgccagtgcg tggattgcgg aaaaaccggg tgcgccactg gccgacgtac
    22381 tggcttgcat gatgcagatc cacgttaata ccccatacct gctcaaccat gcgctggaaa
    22441 gattactgcg tgggcacgga cacgccgcca gcgatatcat tcactttacc gattatgtgg
    22501 tggagcgcgg tagcgacaaa catgttgcgt atgctgcaag caaagcggca ctggataata
    22561 tgacccgctc gtttgcccgc aagctggcac cggaagtgaa agtgaattct attgcgccat
    22621 cgctgatcct gtttaatgaa catgatgatg ccgaatatcg acaacaggcg ctgaataaat
    22681 cactgatgaa aaccgcgcct ggcgagaaag aagtgatcga cctggtcgat tacttactta
    22741 ccagttgctt tgtcaccgga cgcagtttcc cacttgatgg cggtcgtcat ctgcgttaat
    22801 gcagctttat ccagcatata atcaacaccc atgcgcttat cccccaacac gcaacagaac
    22861 caacaaaagc ggcgggcaag cgcatcattc cggtgaaata ccacagcgac accaggtaaa
    22921 caaaataggg aataatcgac cacatactaa aaatgatggt tgcgcgtaag gcttcaatgc
    22981 cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa ggtcgggaaa agtggaatca
    23041 gcccggcgat ataataattt ttcgtttttg ctaaaacacc aatcaacagc actaccagcg
    23101 caccgagcgc ggctttgatt accagcccca tctttttacc ttaacacttc cataacaagt
    23161 catcagtaga atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc
    23221 gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg
    23281 gaagatgatg cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag
    23341 gttaccgtag aaccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga aaatccggat
    23401 ttggtgttac tcgacatcat gctaccaggc aaggacggca tgaccatttg tcgtgattta
    23461 cgcgcaaagt ggtctggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac
    23521 atcctggcac tggaaatggg tgcctgcgac tatattctca aaacgacgcc ccctgctgtt
    23581 ttgctagcgc gtttacgttt gcatttgcgt cagaatgagc aagccacact gaccaaaggt
    23641 cttcaggaaa cgtctcttac tccctacaaa gccctgcatt tcggcacgtt aactatcgac
    23701 cccatcaacc gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc
    23761 gaattattgt gggaactagc tacgcatgcc gggcaaatca tggaccgcga tgcattgctg
    23821 aaaaatttac gcggcgtcag ttatgacgga ctggatcgta gcgtggacgt ggctatttcg
    23881 cggttaagaa aaaaactgct cgataacgcc gcagaacctt atcgcattaa aactgtgcgt
    23941 aacaaaggct atctttttgc gcctcatgca tgggatagtg acaataaaca atcagatgac
    24001 taatatttgt tcatctgaaa aagagaacaa ttaacgaatt caattaataa gagcttattc
    24061 aaattccaaa ccccctacat ttgatatgaa aatgagaaaa aatttctcta taatcgcctc
    24121 gtccctgcgt cttatggcgc agatttttta caacaaactc tttccggcat ttcaggtgct
    24181 gaatgaggca agaggcttaa aaaacggtta caactttcac atatattgtg ctttttttga
    24241 tctgctcatg ggggattttc aaaacggatc aaaagagcga atccctttcc aaaagtgttt
    24301 ttatcgcgat tttcctgggg gctatcattg gtatctccct gcatttcatt cctgagacaa
    24361 gcagtgaaac cgttatcgaa tggtataata ttgctggtaa cggttatgtt aatttattaa
    24421 aacttgttgc cgttccactc atattcattt cgattctttc agcgattaat aagctcgaaa
    24481 at
//
LOCUS       DAEEDS010000058        24185 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEEDS010000058 DAEEDS010000000
VERSION     DAEEDS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24185
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.058"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(25..597)
                     /locus_tag="KLH32_002585"
     CDS             complement(25..597)
                     /locus_tag="KLH32_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBE0139366.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="KLH32_002586"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="KLH32_002586"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBE0139367.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRSGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KLH32_002587"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KLH32_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBE0139368.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KLH32_002588"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KLH32_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBE0139369.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="KLH32_002589"
     CDS             complement(3559..5340)
                     /locus_tag="KLH32_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE0139370.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="KLH32_002590"
     CDS             complement(5333..7105)
                     /locus_tag="KLH32_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE0139371.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KLH32_002591"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KLH32_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBE0139372.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KLH32_002592"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KLH32_002592"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBE0139373.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8664..10356
                     /locus_tag="KLH32_002593"
                     /pseudo
     CDS             8664..10356
                     /locus_tag="KLH32_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="KLH32_002594"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="KLH32_002594"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBE0139374.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KLH32_002595"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KLH32_002595"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBE0139375.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="KLH32_002596"
     CDS             complement(11660..12031)
                     /locus_tag="KLH32_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBE0139376.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KLH32_002597"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KLH32_002597"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE0139377.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KLH32_002598"
     CDS             complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KLH32_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBE0139378.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KLH32_002599"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KLH32_002599"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBE0139379.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KLH32_002600"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KLH32_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBE0139380.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KLH32_002601"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KLH32_002601"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBE0139381.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KLH32_002602"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KLH32_002602"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBE0139382.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KLH32_002603"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KLH32_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBE0139383.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="KLH32_002604"
     CDS             21802..22380
                     /locus_tag="KLH32_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0139384.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="KLH32_002605"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="KLH32_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBE0139385.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6665 c         5828 g         6026 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gctcaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcattca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taataatatt gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc
     8701 tgtggcaacc ttcagccgca aaccgtcacc gtcagcgaac tggccgaacg ctgtttttgc
     8761 agcgaacgcc atgttcgtac gctgttgcgt caggcacagg aggcgggatg gctggagtgg
     8821 caggcgcagt caggacgcgg aaagcgcgga caattacgct ttctggtcac gccggaatcg
     8881 ctacgcaatg cgatgatgga acaggcactg gaaaccggaa agcagcaaga tgtgctggag
     8941 ctggcgcaac tggccccagg tgagctgcgc actctgttac agccatttat gggcggacaa
     9001 tggcaaaacg atacacccac gttgcgtatt ccctactatc gcccgctcga accgctacaa
     9061 ccaggctttt tgcccggccg tgccgagcag catctcgccg ggcagatatt ttccggcctg
     9121 acccgcttcg ataataatac tcagcgcccg attggcgatt tagcgcatca ctgggaaacc
     9181 tctactgaca ggttacgctg ggacttttat cttcgttcaa ccctacactg gcataacggc
     9241 gatgcagtaa aagcctcaca cttacaccag cgattattga tgctgttaca actgccagca
     9301 ctggatcaat tatttattag cgtgaagcgt attgaagtca cccatccgca gtgtctgacc
     9361 ttctttttac atcgccccga ttactggctt gcgcaccggc tggcgagcta ttgcagccat
     9421 ctggcgcatc cgcaattccc actgatcggc actggtcctt ttcgcttaac acaattcaca
     9481 gcggagctgg tgcgcctgga gagccatgat tattaccatt tgcgtcatcc gctgcttaaa
     9541 gcggttgagt actggataac tccgccgctt ttcgaaaaag atttgggaac cagttgtcgg
     9601 catcccgtgc aaatcaccat cggcaaaccg gaggagctgc aacgggtcag ccaagtcagt
     9661 agtggcatca gtttaggttt ttgctattta acgttgcgca aaagtccccg actctccctc
     9721 tggcaggcgc gaaaagtgat ctccattatt catcaatccg gtttattaca aacgttagaa
     9781 gtcggagaaa acctgatcac cgccagtcat gcattactgc taggctggac tattccgcac
     9841 tggcaagtac cggatgaagt caaactaccg aaaaccttga cgctggttta tcacctaccg
     9901 atagaacttc ataccatggc agaacgccta caggcgacac tggcagcgga aggctgtgaa
     9961 ctcacaatta tttttcataa cgcaaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg gcgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacaggg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgagaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcatgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtctttatt ttctcaccac ccagttgtca ctttaatgat aattatttgt taaataattg
    24121 ttttatttcg cattggttat accaattgcc cgccaagact ccttctgcac tccccttttg
    24181 ttata
//
LOCUS       DAEEDS010000059        22679 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEEDS010000059 DAEEDS010000000
VERSION     DAEEDS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22679
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.059"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(5..487)
                     /locus_tag="KLH32_002606"
     CDS             complement(5..487)
                     /locus_tag="KLH32_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0139386.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSGHYHLFFF
                     "
     gene            625..1545
                     /locus_tag="KLH32_002607"
     CDS             625..1545
                     /locus_tag="KLH32_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0139387.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1545..2482
                     /locus_tag="KLH32_002608"
                     /pseudo
     CDS             1545..2482
                     /locus_tag="KLH32_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2500..3138
                     /locus_tag="KLH32_002609"
     CDS             2500..3138
                     /locus_tag="KLH32_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0139388.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3429..4157
                     /gene="mpaA"
                     /locus_tag="KLH32_002610"
     CDS             3429..4157
                     /gene="mpaA"
                     /locus_tag="KLH32_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBE0139389.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4132..5091)
                     /gene="ycjG"
                     /locus_tag="KLH32_002611"
     CDS             complement(4132..5091)
                     /gene="ycjG"
                     /locus_tag="KLH32_002611"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBE0139390.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5210..5716
                     /gene="tpx"
                     /locus_tag="KLH32_002612"
     CDS             5210..5716
                     /gene="tpx"
                     /locus_tag="KLH32_002612"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBE0139391.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5760..7301)
                     /gene="tyrR"
                     /locus_tag="KLH32_002613"
     CDS             complement(5760..7301)
                     /gene="tyrR"
                     /locus_tag="KLH32_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBE0139392.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7449..8510)
                     /locus_tag="KLH32_002614"
     CDS             complement(7449..8510)
                     /locus_tag="KLH32_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBE0139393.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8507..9904)
                     /locus_tag="KLH32_002615"
     CDS             complement(8507..9904)
                     /locus_tag="KLH32_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBE0139394.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10060..11058
                     /locus_tag="KLH32_002616"
     CDS             10060..11058
                     /locus_tag="KLH32_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0139395.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11169..12041)
                     /gene="ompG"
                     /locus_tag="KLH32_002617"
                     /pseudo
     CDS             complement(11169..12041)
                     /gene="ompG"
                     /locus_tag="KLH32_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(12114..13195)
                     /locus_tag="KLH32_002618"
                     /pseudo
     CDS             complement(12114..13195)
                     /locus_tag="KLH32_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13209..13868)
                     /gene="pgmB"
                     /locus_tag="KLH32_002619"
     CDS             complement(13209..13868)
                     /gene="pgmB"
                     /locus_tag="KLH32_002619"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBE0139396.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13865..16132)
                     /gene="ycjT"
                     /locus_tag="KLH32_002620"
     CDS             complement(13865..16132)
                     /gene="ycjT"
                     /locus_tag="KLH32_002620"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBE0139397.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16129..16752)
                     /locus_tag="KLH32_002621"
     CDS             complement(16129..16752)
                     /locus_tag="KLH32_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0139398.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16749..17171)
                     /locus_tag="KLH32_002622"
     CDS             complement(16749..17171)
                     /locus_tag="KLH32_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0139399.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(17193..17968)
                     /locus_tag="KLH32_002623"
                     /pseudo
     CDS             complement(17193..17968)
                     /locus_tag="KLH32_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17986..19038)
                     /locus_tag="KLH32_002624"
     CDS             complement(17986..19038)
                     /locus_tag="KLH32_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBE0139400.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19069..19911)
                     /locus_tag="KLH32_002625"
     CDS             complement(19069..19911)
                     /locus_tag="KLH32_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE0139401.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19898..20779)
                     /locus_tag="KLH32_002626"
     CDS             complement(19898..20779)
                     /locus_tag="KLH32_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE0139402.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWITVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20800..22083)
                     /locus_tag="KLH32_002627"
                     /pseudo
     CDS             complement(20800..22083)
                     /locus_tag="KLH32_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(22097..>22679)
                     /gene="ycjM"
                     /locus_tag="KLH32_002628"
     CDS             complement(22097..>22679)
                     /gene="ycjM"
                     /locus_tag="KLH32_002628"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBE0139403.1"
                     /translation="LVNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHA
                     ILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHA
                     VYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQH
                     VNITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5594 a         6097 c         5524 g         5464 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aggacgcctg
       61 catcaaaacg ttcagaaacg acatcggtca aaccataatc aatggtaagc tgaatatcaa
      121 tttcaggatg cgatttcagg aatgtgcgca tggctggtaa caatatcgtt tttgctgcat
      181 gttctacagt agtaatacgt attgtcccgg atgggcggtt ctgcagatcg ctcagggatg
      241 tcagggcaga atctatgtca tgcaatatag ggccaagaac agacaaaaga tgctcgcccg
      301 cttcagtggg tacaacgctg cgcgtggttc gcgtcagaag ccgcaatccc aatcgttctt
      361 ctatacggcg cactatctgg cttaaagctg actgcaccat gctcaggcgg gctgctgcgc
      421 gagtgaagct acgctcctct gcaacgacga caaacgccat cagatcagcg atttcttcac
      481 gcttcattca gtatctccat acaatctatt aatcatttaa atgataggtc taatcatgat
      541 ttagggtcta atcaacttaa tgatcaatat ctatagtgat atcaccaacc gtcaaaacga
      601 accgacaact ccatgaggaa ctgaatgatg aataataaag tcagcttcac taacagcaat
      661 aatccaacca tctctttgtc tgcagtgatc tatttccctc caaaatttga tgaaacccgt
      721 cagtatccgg caattgtggt atctcaccca ggtgggggcg ttaaagagca aacggccgga
      781 acctatgccg aaaagctggc ggaaaaggga tttgtcacta ttgcttatga cgcatcttat
      841 caaggtgaaa gtggcggcga gccgcgtcag ttagaaaacc catacattcg tactgaagac
      901 gtcagtgccg tgattgatta cctgacaacg ctttcttatg tcgacaatac ccgaattggt
      961 gcgatgggaa tttgtgccgg tgcaggatat acagccaatg ctgcaattca ggatcgccgc
     1021 atcaaggcca tcggtaccgt tagtgcggtc aatattggtt cgatgttccg caatggttgg
     1081 gaaaacaatg tgaaatctat tgatgctctg ccacacattg aagctggctc aaacgcccga
     1141 accagcgata ttagcagcga agaatacgcc attatgccac tggctccaat gaaggaggcc
     1201 gatgcgccga acgaagaact gcgtcaggcc tgggagtatt accacacacc tcgtgcgcag
     1261 tatccaacag caccgggtta cgctactttg cgcagcctta accagattat tacctatgat
     1321 gcttaccata tggcggaagt gtacctgact cagccgatgc agattgtggc gggcagccag
     1381 gcagggagca aatggatgag tgacgacctg tacgatcggg catcaagcca ggataaacgc
     1441 tatcacattg ttgaaggcgc aaaccatatg gatctttatg acggcaaagc ctatgtggct
     1501 gaagcaattt cagtattagc tccgttcttt gaggaaactc tgtaatgaaa catattcaaa
     1561 tccgcaatag tgatatggcc tggcatattg ctgccaatat tcagtttcca ccaaattttg
     1621 atgagtcaaa acagtatccg gcgattatca gcgtccatcc ttttggcagt tgtaaggagc
     1681 agacgtctgg caatatctat ggcaaggcat tggctgaaaa aggctatgtc gttctcgctt
     1741 atgacgccag tttccaggga gaatctggtg ggtagcctcg ttggatagaa gatccgacac
     1801 agcgcgtaga ggatattagc cgtgttatcg attatgcagt aactctgcca tatgttgatg
     1861 ctgaacgcat tggcgtgctg ggtgtgtgtg gggggggggg ggtggctatg ccattaatgc
     1921 cgcgctgaca gaaaaacgaa ttaaagctgt agtgagtatt acgggtgtta atatcgggcg
     1981 tctttttcgg gaaggtttca gcaattatga tccgattggc gtattgaatg caatggcgtc
     2041 acaacgagca aaagaggctc gtggtggaga attacagatc aacgaattac ttcccgccag
     2101 cctggatgca gcaaaagccc atggactgac cgaacgcgat gtttatgaag caactgacta
     2161 ctataaaaca ccacgcgggc aacaaccggg aggtgcgaca aaaatgttgt tctcacatgc
     2221 acagaaaacg ctggcctggg atgccttcgc atttactgaa gtgttactga cccagccggt
     2281 gatggtagta gttggtgaaa aagtcggtgc cttcggtgct tatcgtgatg gactggaagt
     2341 ctatggccgg gcgatggttt cgcaggatcg tcagttagtt tcgttacccg atttctcaca
     2401 ttatgaacta tatgacaaac cggaagcagt tcaggaggcg ctggcaaaag ttattccttt
     2461 tttcaacacc catctgggat aagtttcatg agaaatatta tgaaaaatgt actgattctt
     2521 ggtgctggcg gtcagatagc ccgccatgtg attaatcaac tggcagataa acaaactatt
     2581 aaacaaactt tatttgcccg tcagccagca aaaatacaca aaccctatcc aactaatagc
     2641 cagattatta tgggggatgt actgaatcat gcagcactga agcaggccat gcagggacag
     2701 gatattgtgt atgccaacct tacgggggaa gatttagata ttcaggcaaa ttcagtgatc
     2761 gctgcgatga aagcctgtga tgttaaacgt ctgatctttg ttctttccct cggtatttat
     2821 gatgaagttc caggaaaatt tgtcgaatgg aataatgcag ttattggtga acccctaaaa
     2881 ccattccgca gggctgcgga tgcaattgag gcttctggac tggaatatac aattcttcgc
     2941 cctgcctggc tgactgatga agatattatt gattatgagc ttacctctcg caatgaacca
     3001 ttcaaaggta cgatcgtatc acgtaagagt gtcgcagcat taattacaga tatcatcgat
     3061 aaacctgaga aacacattgg tgagaatata ggcatcaatc aacccgggac tgatggcgat
     3121 aagccattct tcatgtaaat caattatata gataccatcg tactgtacta tgtgcagtac
     3181 gatttattaa acttattaag gtgaactgat caatcagatg cgattaacat gtgtagaacc
     3241 agtaagaata aaatacgcca tgtatataac attacgacaa ttctaatatc tgcacatcaa
     3301 tgaaaatgca aacaacttcc ggcaaaaaag tgatacaaga tcgatgacat agcgttgaag
     3361 aaaaggattt gataatccgc aaaatttgca caacactgcc tgtaatactt cctgaaccag
     3421 agacgatcat gaccgtaacc cgctcacgcg ccgaacgcgg cgcatttccg cccggaacag
     3481 aacattacgg acgttcatta ttgggcgcgc cgttaatctg gtttcctgcc cctgcagcca
     3541 gccgcgaaag tggtttgatt ctggctggca ctcacggtga tgaaaactct tcggtcgtca
     3601 cgctctcctg tgctctgcgg actttgacac cttctttgcg ccgccatcat gtggtgctgt
     3661 gtgtgaatcc tgacggctgc cagctggggt tacgggccaa tgctaatggt gtggatttaa
     3721 acagaaactt tccggcggcg aactggaagg aaggtgaaac ggtttatcgc tggaacagcg
     3781 ccgctgaaga acgtgatgtc gtattactga caggtgacaa acctggctcc gaacctgaaa
     3841 cccaggcgct gtgccagctt atacaccgca ttcaacctgc ctgggtggtc tcattccacg
     3901 atccactggc ttgtattgaa gatcccagac acagcgaatt aggcgaatgg ctggcccagt
     3961 cgtttgaatt gcctctggta accagcgtcg gttatgaaac gcctggctct ttcggcagtt
     4021 ggtgtgcaga tctcaacttg cattgcatca ccgcagagtt tccgcccatc tcctccgacg
     4081 aagccagcga aaaatatctc tttgcgatgg caaacttgct gcgctggcat cctaaagatg
     4141 caattcgccc gtcgtgaact gaagcgccgg ttccacatct accgccagcc aggtcggtcc
     4201 gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa gcggcgctaa tggcacgaga
     4261 ggtacacaac atgcagccca gcatcagact gaaaccttgt gcacgcgctt cagtcgccag
     4321 cgcttccgtc agaccaccgg ttttatcgag cttaatgtta accatctcat agcgcccttt
     4381 cagcgccttc agattgctac gagtatgaca actttcatca gcacaaatcg gcaacggatg
     4441 aataaaattc tccagcgccg catcgtcctg cgcaggaagc ggttgttcaa gcatcgcaac
     4501 gcctaaatcc gccaatagct ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt
     4561 tgcatcaacg atcagcgtgg catcgggcac agcggtgcga attgccacca tccgctcact
     4621 gataagatgg ttatccagct tcactttcag taatttcgcg cctgcctgcc agagtgttga
     4681 tgcactattg gccatctgat caggcgtacc gatgacaacc gtctgtgcag tgataactgt
     4741 ctcgggaagc gttatgccga tcaaatcagc cagcgattgc tgctgttttc gcgccgcgag
     4801 atcccacaat gcacaatcca gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa
     4861 ctcctcccgt gtcagccctt tctctaattg cggcacgacg ctcataattt gcgccattac
     4921 cgaggcatca ctttctccat aacgcggata cggcgtgcat tcgccggtgc ctttaatacc
     4981 ctcttcttcc agttcaacca ccaccacgcg cgcttcactg cgacttcccc gggcaatcac
     5041 aaacggggta tgtaagggcc aggcttcctc aaatacctta acgattctca tctttactcc
     5101 ttgttgtcgc ggtggtctgc ggattaagcg caaatagcgt ttgctgtgtt attgacagtt
     5161 agcataaact aggtgtgacg ttaactatat gtaaacagga aagataatca tgtcacaaac
     5221 cgttcatttc cagggcaacc cggttacagt cgccaattcc atcccgcagg cgggtagcaa
     5281 agcgcagact tttactctcg tggcaaaaga tctgtctgac gtcaccctcg gtcagtttgc
     5341 gggtaaacgc aaagtgctga acattttccc gagtattgat accggtgttt gcgccgcatc
     5401 agtacgtaag tttaaccaac tggcaactga gatcgacaac accgttgtgc tgtgtatctc
     5461 tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca gaaggtctga acaacgttat
     5521 caccctctcc actttccgta acgctgaatt tctgcaagcc tacggtgtgg caattgctga
     5581 tggcccactg aaaggtctgg cagcgcgtgc cgttgtggtt attgacgaaa atgacaatgt
     5641 gattttcagc cagctggtgg atgaaatcac caccgagccg gattacgaag cagctctggc
     5701 tgtactgaaa gcataagtta atatgcctga tggtgttgca ccatcaggca tattcgcgct
     5761 tactcttcgt tcttcttctg actcagacca tattcccgca acttattggc aatcgcggta
     5821 tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg gataattgcg ataaagctgg
     5881 gttaataccg agcgttcaaa acggctggtg atttcgtcca gcgaaccttc catcgcatct
     5941 tcgcccacgg ctaccgttgc ggcgtcataa tccggcaaca aaatatcctg tggacgcagc
     6001 tcataaccgt ccagttgtgt cagtgcgcga tagatagcgt tctttaactg ccgcacattt
     6061 cccggccacg cataacgtgt aagtacagta ttcaggtcag cggccagttt cggacgcggc
     6121 acgccctgct cgtcggcaaa gcgggcgacg aacagctcag ttaacggcat aatgtcctgc
     6181 ggacagtcac gtagcggcgg cagattgagc gtcaacacgt tcagacgata atagagatct
     6241 tcacggaaca cgcctttttg caccagttcg accagattct tctgcgtagc gcaaatcacc
     6301 cgcacatcga catgcacctc atggtcttcg ccaacccgac ggaaagtgcc atcattaaga
     6361 aaacgcagta atttcgcctg catccgtggt gacatttccc ctatttcatc caacagcacc
     6421 gaaccaccgt tcgcctgctc aaagaaccct ttcttccctt ccggagcatg accaaacagt
     6481 tcactctcga ccgcatcttc tggtatagac gcgcaattca gcgccaggta aggtttgccc
     6541 gctctggggc ttgcctgatg gcaggcgtag gcaaaaagat ctttacctgt acctgtgtca
     6601 cccgtaatca gcagcggcgc gcttagcatc gccagtttct gcgcctgttc gacaacgtgc
     6661 ttcattttcg ggctgacggc gacaatttgg ctgaaggcgc tgacgtcctg ggcggcgaca
     6721 ttttgcaact ggcggcccat acgaatcgtt gatcgcaaca tcaccaccgc accggtcagg
     6781 acgtgttgat cattttcatc ctgaagataa acaggcgtaa tctccatcag gaaattctgc
     6841 ccattaataa cgacatgctc gttatgcgaa tcttgcggtt cgctttccag ccaacgtaaa
     6901 aaattaaagc cgttaatcaa ttgtgcggcg gtatggttgc gcaggcgatc caatttttgc
     6961 ccaaaaagct gacagctcgc cgggttcgcc atatccactt tgcttttcat atcgacagag
     7021 agcacaggtt caggcaacgc ctccagtaac gcgctcaacg ccagatgctc acgttcggaa
     7081 ggcatccacg ggacagtacg cacatcggta acacctgcaa tacggcgtat ttcggccatc
     7141 agactgctga aactctcaaa ctccagttca gcaaaattga ggtagattcg cccaatggga
     7201 tcaatctcaa taccgcgtaa atcaatgcct cttagcacga gtagatcgag taattcgcgg
     7261 gtcagaccga gtcggtcttc acaaaagact tccagacgca tgggaacctt cacctgaaaa
     7321 aagaacaatt aatatgatga tatgacacta tttggatagc aggaaggttt ctgtcaacaa
     7381 tcattgacac aaagacggaa aaacgggcac ttcggcgtaa agatatccgg tcagcacccg
     7441 atattgcatt atttttcgct gggcgttttg cctttttgca gagtttcttt cacctgaccg
     7501 ataagctgac gacggaaatc cccaaggcga ggtttgtcat cgtcaatcca cggcagcggg
     7561 cggcaaagct ccatagcttt aatcccgagt cgtaccgtca gaagtccagc accaattccc
     7621 tgagctgcgc gggtagacaa acgagcagcg agatcttgcg acatccagtc catccccact
     7681 tcgcgcacca gttcgctggc tccggcaaaa gcgatattca gcaataccag cttaaacaga
     7741 cgcaaacggc tgtaataccc cagttcaatg ccatacagcg tggcaatgcg attaattaaa
     7801 cgcagattgc gccaggcgat aaacgccata tcgaccaacg ccagcgggct gaccgcaatc
     7861 atcaacgttg attccgccgc cgaatggctg atttcgcgcc gcgcctgggc atctaaaact
     7921 ggctggacca aatgcgcata caggctgacc acttcacgat cgttctgcgt ttcatggatt
     7981 gaggcatacc agcgttgcag cgccggatgc gactgatcaa tacccgcctg ctgcgccagt
     8041 ttttcgcaaa atgcgcgacc tttgcccgtg ccgtggctat gcaataaatc acgcgcttcg
     8101 tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc gccactctgt taccacagaa
     8161 cctacgccag cgccgataat caatgcccca gcggcacatc cacccagcgc tacccagtcc
     8221 tgggtttgcc aggcattcat tgtccactgt ataccctgcc cgacaacgct tgcgccaaac
     8281 agagccagcc cgcccatcac cattttgcgc cacaggctgc gtttcggacg taacgctgcg
     8341 tccattaccg cttcgacttg cccctcttct tcctgcgctt cgtcgagcgt ggccggggca
     8401 aaattttgcg cctgattttc gtcaaaggtc tgctgcgccc tgaattttgg attctgttcg
     8461 acctccagcg gaccgtcgaa atcaatacgt ggttttaacg gttcggtcat cgcaatttat
     8521 ctcctattaa aaattccagc gcggcatcaa gacgaatatg cggcagcggt ttgtcgacat
     8581 ccatcacctg cgggcgaaac gcttcaaact ggaacccttg cttgtcccag aacgcctgac
     8641 cgggcaaacg cgcaggaact tcaccggggt aaacggtgag aggcgcacca tcgctgagcc
     8701 ggttaccgcg caatgccgga attttctcgc cgttgacgtc aataatgccg ctggtggtcg
     8761 cctgcaccga ggccagcccc aggcaatcca tactaatcct ttcaaacgcc gcattttgcc
     8821 aggcatcctg aatcagttgt tgcagtaatg aaaccatatt ggcgtgctga tcgaccgtta
     8881 catggtccgc tttcatcgcg gcaaacagca gtttgtcgat aaccggcgag aataagcggc
     8941 gaaacagcgt gcgctgcccg tagtggaaac tttgcatcag ctgcgtcagt gccagacgca
     9001 tatcattaaa tgcctgtggc ccactgttga gaggttgcag gcaatccacc agcacaatct
     9061 ggcggtcaaa gcgcagaaaa tgattcttat agaacccctt caccactttc tcgcagtaat
     9121 aattaaaccg ctcgcgcagc attccggcat tggtgtgctt atcggcctgc gccagtttgg
     9181 actcgtccca ggcatcgaca tccggccacg ggaagaattg cagcgcgggc gcacctgcca
     9241 tatctcccgg caagacaaag cgcccaggct gaataaagtg cagcccctgc tgtttacagt
     9301 ggtggagata atcggtccac acggcggcaa tgtctgccag ccggttttcg tcggcaggcg
     9361 ctagcgggtc aagtccttcg cacatcattc gccatttcgc cgaccattcg ccgcgctgac
     9421 cattgagtaa gcccgtcatc tggcgcgacc agcttaagta atcctgcgcc agcatcggca
     9481 ggtcgagcaa ccattcgccg ggataatcca caatttccag atacagcgtg gaggtatcct
     9541 taaagtggcg cagcagcgaa tcgttcgatt taaaacgtaa cgccaggcga atttcactga
     9601 cgccgcgcgt aggcgtcggc caggcgggag gatcaccata taactgcgcc agtccttcat
     9661 cataggtgaa gcgcggaatg ccaaagtcac gctggggaat gcgttttacg cccagcaagc
     9721 gctcttcacg caccgcgctt aacagcggca aacgtgctcc ggcatgaata ttgagcaact
     9781 gattaaccat cgcggtgata aacgctgttt tgccgctgcg gctaagtccg gttacagcga
     9841 ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt gagttcattt ttaagtcgct
     9901 tcattgctgt cctgtgctgc tctgaagacc agaaaaaaaa tcattcgtaa ggatatgaat
     9961 gtgggggcca ggccattgat tatcaactgc ccgtgttgtc atcgacgggc gaactccata
    10021 agatgtgcag cattcacgtt cggacggagt tcctgaccta tgtcccctac tatttatgat
    10081 attgccaggg ttgcaggcgt atcaaaatcc accgtatcac gcgtgctgaa taagcaaact
    10141 aatatctccc cggaagcgcg cgaaaaagtg ttacgggcca ttgaagaatt acagtatcag
    10201 ccaaacaagc tggcccgcgc gctgacctct tcgggttttg atgccattat ggtgatttct
    10261 acccgttcga ccaaaactac ggcgggtaat ccgtttttct ctgaagtttt gcatgccatc
    10321 accgccaaag cggaagaaga aggtttcgac gtgatattac aaacgtcgca caatccggca
    10381 gaagatctgc aaaaatgcga aagcaaaatt aaacagaaaa tgattaaagg cattattatg
    10441 ctcagttcgc cagcggataa gtcatttttt gcccaactcg ataaatacga tattccggtg
    10501 gtggtaattg gcaaagttga aggtcaatat gcccatgttt attctgtcga taccgataat
    10561 tttggtgaca gcattgcgtt gaccgatgca ttaattgaaa gtgggcatca aaatattgcc
    10621 tgcctgcatg ctccgcttga tgtccatgtt tcggttgatc gcgttaatgg ctataagcaa
    10681 agtctgtccg cgcataatat tgcagtgcgt gataaatgga ttgttgacgg cggttatacc
    10741 catgaaacag cactgcaagc agcacggcaa ttattaagtc agtcaccgtt gcctgaggca
    10801 gtgtttgcca ctgacagcct gaaattaatg agcatttatc gtgcggcggc agagaaaaat
    10861 attgctattc cgcagcagtt agcggtggtg ggttatagta atgaaacgct gtcgtttatt
    10921 ttaacgcctg caccaggcgg catcgatgtt ccgacgcagg agttaggtca acaaagctgc
    10981 gagttattat tccgcttaat ttcgggaaaa ccgtcaccac agaatattac cgttgccacg
    11041 catatgacgc tgaaataaat accagctacc ttgcctgaat gccggatgcg atgctggcgc
    11101 atcttatccg gcctacattt atagatcgga taaagtactc gcgccgcatc cggcaattta
    11161 gcccattatc agaacgagta atttacgccg acacctgcat aatggaattt atcgctgtcg
    11221 ccttcgtcgt gatcctgcca ctcaaacgcg tattccagcg aaacggaaag tccgttctgg
    11281 aaatcataac cgtaaaataa acccacacgg ttaaaatcat ggccttcacg ttcaatatca
    11341 tcctgccagt cccagttact ctagcgatcc agcccaatgc gcgtatacgg cgtcaccgag
    11401 tggttgccga gcgtcagcgg caaataggcg cgaatttctt gtgtggaaaa ctcaccgtta
    11461 ttgcggctgt cgtccatatt gaagccgcgc tcgagataat agttcactcg caaagcaacc
    11521 gtttcgttga aggtatattg cagacctgtt tccgtttcga cacgggtatc agcgtaaccg
    11581 gtcgtgttca aatcgttggc aaatttatac atcgacaacc aaccgttgaa acgtaaatca
    11641 tcagtcagtt tcacatccca gtctggcgcg attttccagc gctgcatatt cgccgtgtct
    11701 ttgcccggtt catcaacgta gtgataacca taattacgga aaccgccggt caggccgaaa
    11761 ctgaaatcat cgctttcgag gaactgataa tgcacctcca gctccgggcg atcaaacctc
    11821 gttccacgtt tacccgcgct ataatctacc ggcccttcct gataataggc cagagcaatt
    11881 ctccacggcc cgttggcggc attaaaatag actgaaggct ccgccagccc atccatatct
    11941 tcgccataac cctcgacgtt ttctatttcg tacatcgcac cgatattaaa gtgccagtcg
    12001 ctcttttcct cggcctgtgc gcaggccatt cccgcacaca tcaccagtgc ggtacagggt
    12061 aataactttt tttgttcctt gtattatcct gggggaatgc cttccccctg tatttagcga
    12121 attgctattt ccgtttctgc atcaaagaaa tgacatttcg tcatatcaaa atgaatagtg
    12181 atattttctc ctgcatgata atcatttaac gcaccagcac ggaccactaa ctcgtgcccc
    12241 caaccgtggt gtagagcata aattccgcac cggttaattc tgccacgcta attttggcgg
    12301 aaatgttatt ttcctcttgc gcgtccggat gaatatcttc cgggcgtatt cccatcacga
    12361 tgggcttatg caaactttcc tgtgttttca gaaccgctaa tttctcttcg agaatggtta
    12421 atttaagcgt ttccgtaacg aatttatcgc catcgatcgt gccgcgaata aaattcatcg
    12481 ctggtgatcc aataaatccg gcaacaaaca tattcgcggg ttggttataa acggttttcg
    12541 gcgcacctac ttgctgaaca atcccgtctt tcataatcac aatccgcgcc gccatggtca
    12601 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt gttcagtttc tgatgcagct
    12661 tgctgatctc tgcacgcatt tgtacgcgca gcttggcatc aaggttagag agcggttcat
    12721 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
    12781 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
    12841 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
    12901 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
    12961 cgatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
    13021 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgattta ccgcagcccg
    13081 acgggccgac aaatacgatg aactctttat cggcaatttc caggttgaag tccttcacca
    13141 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
    13201 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
    13261 gagggcaaca gtaattgcgc cccggttaag cccgcaccga tccccaccga gcgcataccg
    13321 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
    13381 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
    13441 agttgggaag catccgcgca gaaggtgaaa aactcgcgca gctccagcgc cgctaaaatc
    13501 gtcggcgcat tcagggagac ggaagccagc cctaccggaa tctgctgtgc acggagctct
    13561 gccagcaaat tgcgaatacc gggcagcaca gcattaaccg tcagctcgcg cagtgagtgg
    13621 acgtaaagca gatttttgcg atacgccagt tgcgcccgct cctgcgggtt aaagtcgccc
    13681 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttaga
    13741 gattcgttaa actgcgcatc aatactgatg ccaatttcag cggcaatctg ctgccacgcc
    13801 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat taccccttgc
    13861 agtttcattc atcctcctga tgcgtggtag cggtcccatt gaagggcaaa ataaaatcac
    13921 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
    13981 cgggcgcaga agttcgaatc gcaatacgct gcgcgtcgag agtgacctgt aattcgcagc
    14041 cctgccagaa cagagggaaa gacaactgtt gccactgctc aagtaacgcc ggatttagat
    14101 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
    14161 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
    14221 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcagcaa
    14281 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
    14341 gcagattggc aagacacgat gccgatgaga actgctctgg cagcatgtaa ttgagcatca
    14401 ccacatcagc ttgtttgagg atctgcatct cgttcacttc tgcgcgtgaa taatccagca
    14461 gaatggtttg cttccccgcc gccgctttgt atttcgccag attaatcgcc ggcttagtca
    14521 taaacgaatc atcctgcggc aaaacaccgt cgggctgaat ttctggcagc cgcagttctt
    14581 taaggaacat ttcggcacga tgaataaacg cgtcgtcact acagccaaac tggcgggcaa
    14641 tactcagcgc ctgctggacg ttgtaataca ccatatagct ggtgaaggcg ttattattga
    14701 catgttcggt atattcgtct ggcccaataa catcatgaat ttccagacgg tcgttaaccc
    14761 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
    14821 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
    14881 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
    14941 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
    15001 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
    15061 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatggaac ggcagcaaaa
    15121 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
    15181 caatactgct gcgctcgccg tgagccggcg tcatgatgcg cagatgataa agcgcataat
    15241 ctaacgcttg ctgatcgtgc gcatcgccgc catttaccgt gatacgacgt ttctgccacc
    15301 attgacgcca gttttctgtt gatgctgcaa gatgttggtc gtaactctgt tgcgcgcaca
    15361 tttcaagctg gcgaagcgac acgctgcccc actcgtctaa agcagcttgc ctgtcatccc
    15421 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgtac
    15481 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
    15541 cctggcagca acaactgatg gccacatcac tgcgcccatc ctgggtggtg tagatcccct
    15601 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
    15661 tggtttgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggca
    15721 taatagtgat ttccagcgca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
    15781 cgatggtgta acctgcgccg ttgctggtac gccagacaac gctacggcgt aattctccac
    15841 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
    15901 tggcaatctc catccccaag atatcaggca gattcaccag ttcgttgatt tcaccttttc
    15961 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
    16021 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
    16081 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
    16141 acgcgtccct gtttctgcgg attgatataa cgccgccacc agttgctgga tgatgtaccc
    16201 ctgctcggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
    16261 gctgcgcaaa tggcggttgt cgtctgccat ttcccgttgc atcagcgtca ttaattcacc
    16321 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccggctttat caccacaaaa
    16381 gctgacgttc ataatcaact gttcgcggat gttgagtgca aatgacgttt ccagccacag
    16441 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
    16501 cggatcccac tcgccaaatt gaccacagct cttttgcgtg ccgatctttt gaaaactatg
    16561 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
    16621 aatgccgatg tcgatcagca ggccaccacc ctgcagttct ttattggtaa agacacccca
    16681 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
    16741 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaacgatg gtgaaagtcg
    16801 taggccagca ccttgcccag tttgcgcgcg gtatcgcaca tttcccacgc ctgttctgtc
    16861 gtcatggcgg gcggtttttc gcacatcaca tggcagcctg cttccagtgc catcagggta
    16921 tgttcgtaat gaaaacggtt aggtgagccg acgctaacca catcaggttt caccgccagc
    16981 agcatggcct gcggatcgtc ccacacggat gcattcccgt atttttctgc cagcgcctgc
    17041 gcctgggaaa ggcggctgtc acagacagcc accagttcca gatcgttgcg ggtgcagtag
    17101 tacgaagcat gaaccttatc cgccacctgg cccgcgccta ttatcgcgac ccgcagcgga
    17161 gagcttgtca ttgcactttt cacaaagacc tcttagcagg tacgcaacca ggccaacgaa
    17221 tcacggtacg cctgggcagg atcttctgcc cggatacgcc cttcatacac cacataaccc
    17281 tgatagttat cggcgcgcag ctgttcaaac agcgcgtgga aatcgagggt gccactgccc
    17341 ggctggtaac gatggttatc ggcaatatgc acatgaccga gcaagtcacg gttgtcgtgc
    17401 agcgcctgcg ccaggttatc ttcttcgatg ttcatgtgat agaaatcgcc gataatctgc
    17461 acatgtttaa gattgttttc gacgatgtaa cgacgagcat cggcgagggt gttgatcata
    17521 tgatcctgat agcggtttaa cggttcgaga tacaccacgg ttccggtacg cgcggcgacc
    17581 tgttccagta cgcgcaggga atcactcacc agtttgcggt cgccgtccag gctacgcggc
    17641 gaggtcatcg gcggtaagcg gaaggtaaac atgccccacg ctgccggaaa ccccccactt
    17701 ctgccagcgc ctcgagaatg cgttcgatct gctttaagcc attaagacga cgctcttcga
    17761 taaagtcacc aatccagccg tcatagccgc cgcaggcggt ggtcactggt aaaccggttt
    17821 ctttgatcgc cgctttgact tcttcgatgt tgttgaccag cagtttgccg tcaatctcaa
    17881 aaccatcgaa gcccatctct ttgatataac gaaatctctc gcggatattt tccggaaaga
    17941 atgcctgatt ctgtgtgccg attttcattt tttcattcct ttgaattaaa acgtaacgcc
    18001 cattttgatg ctctgttccg gatgctgatc gacatactgc atgtagcttt ccgggctgtt
    18061 ggcaaaggtc actaccgggt cgattaaatc ttcgcaattg agataaccgt tcatcagcag
    18121 ttcccagcag gtttcttcaa tacgcttacg gctccagcgc ggataatccg ggttcggttc
    18181 gctgcacgcg cgggagaaaa caattttggc gttattgaaa tgcgcttcgc gtccgaggtt
    18241 aaaaccttca gcaaatggtt tggcaaacgc gacataggag atggtgccgc cgtaggccag
    18301 gccgcgcagc gccgattgca gcgcgtcggc gtaaccgctg gtttcgataa tcacatcagc
    18361 cccctgcttg ccggtcagcg ttttgatctc tttgcctacg tcagtgccaa tggggttaag
    18421 gcagaaatca gcaccgtggc gacgggcaat atcacagcga tgggcgatag gatcgacgcc
    18481 aattaccacc gatgcgccag cgcgtttcgc cagttggatg gcaatttgac cgatcgcgcc
    18541 aagccctacc accacgacaa aatcccctac gcgtacgttg gcatcgcgca cgccgctcat
    18601 ggcaaactgc gccgggtcgt aacagacggc gtttttccag gagctgcctt gcggcatttt
    18661 gcgcagcttg tagttattca ctgcgttaat gatgaccgtc tcggagagcg ggccgtagcc
    18721 gcatacgctg tcgcccaccg cgtagtctgt aacgtcgctg ccgcactcga taatgtcgcc
    18781 aaccaccatg ttgccaagct ggaatttgcc aaactcaatg ccgcgcggcg catctgccgg
    18841 acgcggggtg aacatctgcc actcgccgtt aaagtcttca tcgataaacg ggctggcggc
    18901 gcggaagtcg accacttccg ttccgtgttt cggtgcgccg aaacgcacgc ggatcttcac
    18961 ttcattggct aaaatcgccc gatcttcata ctcaaccagc gcagcaacac gcggtgctgt
    19021 ggctactaac tttttcatgt aaaactcctt gtaaagaaac tggccgcttt agcccttcac
    19081 gccaccggcg gtcaaaccac tcttaataaa acgttccgaa agcgcataca taatcaccac
    19141 cggcaatgcg gtcaccagcg aggccgccat catccgtccc cagatgtaat caggcgtact
    19201 gaacagcgcg ttcaggccca ctggtaaggt gaaattgctg gcgctggaga ggaaaatcga
    19261 cgcaaacaga tagtcgttcc acgccaccat aaagcagtag acgaacaccg atatcagccc
    19321 ggacatcgcc aggggcacgg tgatacggaa gatgatttgc agacggttga ggccatccat
    19381 catcgccgct tcttcgatct catcagggat ggtgtcgaag taacttttca gcataaacac
    19441 ggcggtgggc agggtttgcg tcaccatggt aatgatcagc gccatctcgg tgtcgtaaat
    19501 accaagcgcg gtgatgattt tgaacagagg caccaccagc aaaatgccag agaacatata
    19561 caccgtgtaa aagctggcgt tgatggtcat ccggccttta aagcgcaggc gggaaagcgc
    19621 gtaagctccg agaatgccga gaaataccgc caccacggag gaaaccaccg acaccaccat
    19681 gctgttacgg aagtagtcga caaacgggaa aatcatcggg ttaaaaatgt cgacgtaatg
    19741 ctccagcgtc cactgttgcg gcagcagcgt aggatgcagc gatatcgcct ctttcgcgct
    19801 tttgaacgag gtcatcagca tcacaaaaaa cggaaacagc gtgatgatga gaaacagcgc
    19861 cagcccgcag taaaaaccga tacgactgag cgtgcgttta tttgttgcca ttgaggttca
    19921 cccgtttcct ggtcagcaga atgacagcga aaataatgat gaagagcacc accgagatcg
    19981 ccgccgcttt ccctaagtca ttaaaggcaa aggcggtttt gtagagatag acaccaagaa
    20041 tatcgacctt ggttgtcagc agataaacat cagcgaacat gtagaacatc cagatggtgc
    20101 gcagggtcac caccgtcgcc aggaccggca taattgcggg cagcgtgacg atgcgaaacc
    20161 gctgccaggc attagcgcca tccatctctg ccgcttcgta cagcgatttg tcgatggtct
    20221 gcaaaatcgc cagaaacgag ataaaggcat acgggaagta gcgccagatg gcgaacagca
    20281 ccaccagcac aaagctactg cccggattat caaaccacag cggtgcctgc tcatagagat
    20341 gcagaagatc gacgccgagg tagttcacaa tgccgtagcc gttgttgaac atgtatttcc
    20401 aggcaaacac cagcgaaatg gacggcgtta cgtaggagag gatcaccagc gaacgcgcgg
    20461 ttttgcgcag gcggaactcg cggttgaaaa acatcgccac ggccagcccg agcacggtgc
    20521 tgcccgccac caccagcgcc gtataccaga ccgtaatcca cagcgagtgc cagaagccgg
    20581 gatcggagag aatgcgcaca tagttactca ccccaacaaa cgttgactcg atgttgggat
    20641 tgagcggcag acgtaaaaaa ctgatttcga tattcgacac catcggccac gccaccagac
    20701 cgcccagcag taataagctg ggcgcgagaa gcagcagcgc aaacggcata tcggaacgac
    20761 ctgaaaaaag cctgttcatt gcggattcct gtcctgggtt tagtgctgtt cgatcagttc
    20821 atccagcttt ttctggctcg cttgcagcgt gccagggaga tcggctttac ccacggtgac
    20881 gttatgcacc atggagctca ccacaccaga acccgtcaca tcgcccatgc gggtaaagtt
    20941 tttatcccct actgcgccaa agacctgaat atttggcagt tcaccgatta gctgattcgg
    21001 tagttcaccc agcgccttaa taacgtcgtt gtctttccag gtggcggtag tgacaaccgc
    21061 tttattcacc ggcagcgcag cacctggcga catcatcacc cagtcggcaa tgttgtccgc
    21121 ctgctccata aaggtgacaa atttttctgc tgcttcggtc ttttgtccgg cggtaatggt
    21181 cagcgaggtc aacatgccgt agaccgcaga gtttttctcg gttggcacca cgaaaccgac
    21241 gtttttcggg tcgccttctt taatcacagc cggaaggata taggtggagt aaatcgccat
    21301 cggcgcggtg ccgttcataa aggcgtcttt cacttccatg atgtcgttag aacccggcat
    21361 ggtgttggca gcaaggtcgc ggtaataggt cagcgcctgc atcatctctg gtgtatcaag
    21421 ggtgattttg ccttcggcgt taaagacgtt ggcctagttg gataacgcaa actgggagaa
    21481 ggattgttcc gtcagcacgc tttctgccgt aggcagcgca ataccgtatt ttttattcgc
    21541 cgggtcattc agtttctgtg caacgtccag cagctgttgc cagtttttcg gctcctccag
    21601 ccccgctttt gccagcacat ctttgcgata ccagatgtcg ccaatccagg cgctgacagg
    21661 aacaccggtc catgcgctac catcttcggt acgcacaata cgcagtacgc catcgtaaaa
    21721 cgcgccttca ccaacgttgc tgatgaccgt ggcaaccgct ttgcgatcga taagctgctc
    21781 tttatccata actttggcgt agtcatggct ggtttcgatc acttccggca gcgagccgct
    21841 acgtgaaagg gtgatgactt tggtgttatg agcgtcttct tccacgggca cttgtttaac
    21901 ggtgatacct gggttttctt tttcaaaacg ggcaatcaat ttgctgataa ctgcctgacg
    21961 ctcctgctcc accgaagaat gcataaattc gatggataca tgggttttat tttcttcttt
    22021 acagcctgaa atcagggcgc aggaaaccag tgctgataac agcacgattt ttgatttaat
    22081 catgagatgt tcctttttat ttaatccaca taacctgcca ggggcgcagc gttatttcat
    22141 tacccaatat atcaacttca ctaatcagat cccgaccatg cagatcagta atatttacat
    22201 gctgaatatt tttactgaca ttaaacaatc cagtcagaca attaccatca gcgttacttc
    22261 tttgaatacg cattacggat gaattaaccg tatcgatggt aaaattatta tccggatgaa
    22321 actcgttatg gctgcgacga agtgtaatta aacgcgacaa ctcatgatat accgcatgcc
    22381 ttaatgtagc ttcatcgttc agttctcgtg ttatctcttc actgtgatat tttttacggt
    22441 taatcgcacg gttatatccg agtttttcga cacctgcgta atcattacgc gagccaagaa
    22501 tactttgaat atatatcgcg ggtacaccgg ggaaacttaa caaaatcgca tgggcaagga
    22561 taaacctggt gcaacgttct tcatcgctac tctcgcggcg gcttaacgca ttcatatagg
    22621 tgacattcat ctcataggga ctgcgcgtgc cgtcgggatt atttttccag tttactaat
//
LOCUS       DAEEDS010000060        22514 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEEDS010000060 DAEEDS010000000
VERSION     DAEEDS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22514
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.060"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..603)
                     /gene="capU"
                     /locus_tag="KLH32_002629"
     CDS             complement(<1..603)
                     /gene="capU"
                     /locus_tag="KLH32_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBE0139404.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
     gene            complement(606..1448)
                     /locus_tag="KLH32_002630"
     CDS             complement(606..1448)
                     /locus_tag="KLH32_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE0139405.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1634..2351
                     /locus_tag="KLH32_002631"
                     /pseudo
     CDS             1634..2351
                     /locus_tag="KLH32_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3024..4532
                     /gene="ltrA"
                     /locus_tag="KLH32_002632"
     CDS             3024..4532
                     /gene="ltrA"
                     /locus_tag="KLH32_002632"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBE0139406.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            4613..5100
                     /locus_tag="KLH32_002633"
                     /pseudo
     CDS             4613..5100
                     /locus_tag="KLH32_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5183..5440
                     /locus_tag="KLH32_002634"
     CDS             5183..5440
                     /locus_tag="KLH32_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139407.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(5998..6765)
                     /gene="fecE"
                     /locus_tag="KLH32_002635"
     CDS             complement(5998..6765)
                     /gene="fecE"
                     /locus_tag="KLH32_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBE0139408.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(6766..7722)
                     /gene="fecD"
                     /locus_tag="KLH32_002636"
     CDS             complement(6766..7722)
                     /gene="fecD"
                     /locus_tag="KLH32_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBE0139409.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(7719..8717)
                     /gene="fecC"
                     /locus_tag="KLH32_002637"
     CDS             complement(7719..8717)
                     /gene="fecC"
                     /locus_tag="KLH32_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBE0139410.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(8714..9616)
                     /gene="fecB"
                     /locus_tag="KLH32_002638"
     CDS             complement(8714..9616)
                     /gene="fecB"
                     /locus_tag="KLH32_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBE0139411.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(9661..11985)
                     /gene="fecA"
                     /locus_tag="KLH32_002639"
     CDS             complement(9661..11985)
                     /gene="fecA"
                     /locus_tag="KLH32_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBE0139412.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(12072..13025)
                     /gene="fecR"
                     /locus_tag="KLH32_002640"
     CDS             complement(12072..13025)
                     /gene="fecR"
                     /locus_tag="KLH32_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBE0139413.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(13022..13543)
                     /gene="fecI"
                     /locus_tag="KLH32_002641"
     CDS             complement(13022..13543)
                     /gene="fecI"
                     /locus_tag="KLH32_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBE0139414.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            13841..14023
                     /locus_tag="KLH32_002642"
                     /pseudo
     CDS             13841..14023
                     /locus_tag="KLH32_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14017..14121
                     /locus_tag="KLH32_002643"
                     /pseudo
     CDS             14017..14121
                     /locus_tag="KLH32_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14461..14718
                     /locus_tag="KLH32_002644"
     CDS             14461..14718
                     /locus_tag="KLH32_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBE0139415.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERD
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(15038..15169)
                     /locus_tag="KLH32_002645"
                     /pseudo
     CDS             complement(15038..15169)
                     /locus_tag="KLH32_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15451..16809
                     /locus_tag="KLH32_002646"
     CDS             15451..16809
                     /locus_tag="KLH32_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBE0139416.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(17048..18433)
                     /locus_tag="KLH32_002647"
     CDS             complement(17048..18433)
                     /locus_tag="KLH32_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBE0139417.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(18483..18830)
                     /gene="tnpB"
                     /locus_tag="KLH32_002648"
     CDS             complement(18483..18830)
                     /gene="tnpB"
                     /locus_tag="KLH32_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBE0139418.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18827..19207)
                     /locus_tag="KLH32_002649"
     CDS             complement(18827..19207)
                     /locus_tag="KLH32_002649"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBE0139419.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(19562..19996)
                     /locus_tag="KLH32_002650"
     CDS             complement(19562..19996)
                     /locus_tag="KLH32_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBE0139420.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(19984..20385)
                     /locus_tag="KLH32_002651"
     CDS             complement(19984..20385)
                     /locus_tag="KLH32_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139421.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(20645..21214)
                     /locus_tag="KLH32_002652"
     CDS             complement(20645..21214)
                     /locus_tag="KLH32_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBE0139422.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(21959..22102)
                     /locus_tag="KLH32_002653"
                     /pseudo
     CDS             complement(21959..22102)
                     /locus_tag="KLH32_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
BASE COUNT         5422 a         5890 c         5978 g         5224 t
ORIGIN      
        1 ttcgtgccct ttttccgggc gcaacattcc tacctgagca attacggggc atcctcttcg
       61 ggacgccagc caggaaagaa cactcggagg caaactgttt cgggaatcaa cgtataattc
      121 ctggaaatca aagccaggag gcacaacagt aacccgagtt cgacatcctt cctgctccag
      181 atgtgtcttc atatttgttc cgggaacaat cacctcatcg caaaaatgat ttattgaaaa
      241 aaattttgtt tttcgtgtca aatatgtctt ttgcctgata attctgaatg gatgtttcca
      301 ggtaaaaaac cgtaccaaac caacaatatt actatcatgt ccactgtgac aaacgattgc
      361 atcgggctga aaactatgaa ctattccgag taatctccat acagtaggaa tgtgaagact
      421 gtttcgaaat aacgcgaatg tgatatcaat tccaaactta ctggcttcaa aagcaatttt
      481 gctgttctcc ctgcagacaa gcagaactga atgccccatt ttcttcaggg ccttcatttg
      541 agcaacagcc tgtaattcct gaccacctat attcggtgat gattccgtaa atagtatatt
      601 cattattagt catccggtaa tcttggcccc ttatgaaggg ccagcaacga tgaaaaaaac
      661 ggtgttgtat aataaaaaag tcgtcttttc agccatacgc aattttcccg ttccttggtg
      721 cttattcttc ctatccgtgt agttccttta gcaggagcat tcattcttct ttctgttgta
      781 tataaataat ggaatccaag ttcttcagcg atctgaatat aatccttgtt ataataacct
      841 tccggccaac acaaatgttt actgcatttt cctgtcattt tttttaaata ttctctcccg
      901 ctcaataagt cctgccgaag atgtttacac tgttcttctc gtgaagtgaa ctttttatcc
      961 caacgtgtat gagtatgtgt atgaacatga aattcgacca gaccagattg caacatttcg
     1021 ttcacttcag accagcgaag cataacatta tcagcattcc cggttgctat ttgatgttcg
     1081 caatcgcgat gagaatattc ctttccagga gagtgacgaa ccggaccatt tccaataaaa
     1141 ctggtaatta aaaaaatatg tgccttgaga ttaaactcgt ttaataaagg atatacctga
     1201 aaccagttgt caaggtatcc atcatcaaat gtcaacataa cgcttttacg gggaagtttt
     1261 ccgccccgat aaaaaaactc cagttcatct gatgacagtg ttttccagtt gttttcagcc
     1321 aaccatttca tttgtttccg gaatgtctcg ggagaaagtg taaccagtcc tgggcaatgg
     1381 ctgacatgat gatacattaa taccggaaca tgatttgctt taaacaaaat gccccctccg
     1441 tttaaaattt aaagtttctg gcacccagta agtagagata aaaggaaagc ctaataaagg
     1501 tggtgaactt tatatactac taaatcaaac atcaacaatt tataaacaat aatatattat
     1561 catatctttt aaaaacggct cactaagaac cttgaggtgg cacactaaat ttagctacct
     1621 gaacagaggt gatatgctca cctcagaaca acacaggtgc cataatgaaa aaagaaattt
     1681 cagcgcagag tttaaacgcg aatccgctca actggtcgtt gaccagaaat acaccgtggc
     1741 agatgcagcc agcgctatgg atgtcggcct ttccacaatg acgcgatggg tgaaacaatt
     1801 acgtgatgag cggcagggaa aaacaccaaa agcctccccc attaccccgg aacaaattga
     1861 aatccgtgag ctcaggaaaa agctacaacg tattgaaatg gaaaatgaaa tattaaaaag
     1921 gctaccgcgc tcttgatgtc agactccctg aacagttttc gataatcggg aaactcaggg
     1981 cgcgttatcc tgtggccact ctctgccatg tgttcggggt ccatcgcagc agctacaaat
     2041 actggaaaaa ccgtcctgaa aaaccagacg gcagacgggc tgtattacgc agccaggtac
     2101 ttgaactgca tggcatcagc cacggctctg ccggagcaag aagcatcgcc acaatggcaa
     2161 cccagagagg ctaccagatg gggcgctggc ttgctggcag actcatgaaa gagctggggc
     2221 tggtcagttg ccagcagccg actcaccggt ataagcgtgg cggtcatgag cacgttgcta
     2281 tcccgaatca tcttgagcga cagttcgccg taacggaacc aaatcaggtg tggtgcggtg
     2341 atgtgaccta tagtgtgccc ggagttcagg gcgggcatgg atgcttaaat gaaccgcgag
     2401 tctgtctgga atattgaacc ggtaactcac gatgagaaac ccaacaatcc caccgggtgt
     2461 gacggtggag aacctgagcg gcagtgacct gcggcatgcc cgcagggtga tgtaacccgc
     2521 tgacaacggg gattgaggcg agatcactaa gccgagatga tcctcaaggt taagtactga
     2581 aaggctgaag aacatgaacc cgttaatccg cctctgtggg ttgaaaacgt caccacggcc
     2641 tacgtgatct gacaggccgt gcaggaggaa ctggcagtga tacgtaagca ctgccggtcg
     2701 aaggtgtttt gacatgtatg cgaaacaccg gggcagcagc gtctatcacg cttgcgttgc
     2761 tgacttctgc caacttgcgg caagcaagga taaagagtgc gacgggcagc ctcctcagta
     2821 tgcctgagtc caggcaggta aaccggggaa ggtcagcgac ggatgttaag ggggcatggc
     2881 tccgatgacg cgctgactgg cggagcttcc gtagtagtcc gcgatgggga aagcccatta
     2941 catggcgaag ggaagcagtt tgaatgtgtt tgcgacgtga attaactggc ctaacgaggt
     3001 gaagaccttt gataatcagc gaaatgcaac gcaagcttgc cacatgggca gccaccgatc
     3061 cgtccctacg gattcaacgg ctgctgcgtc tgataacaca accagaatgg ctggctgaag
     3121 cggcgcggat cacgctttca tcaaaggggg cgcatacccc cggcgttgat ggcgtgaaca
     3181 aaacaatgct acaggccaga ctggctgttg agctgcaaat cctcagggat gaattactct
     3241 caggccacta ccagcccttg cccgccagac gggtttacat ccctaaaagc aacggcaaac
     3301 tgcgaccact gggtatcccc gcgttgcggg atcgtattgt tcagcgggcc atgctgatgg
     3361 cgatggagcc gatatgggag agtgattttc atacgctctc atatggcttc cggcctgagc
     3421 gcagtgtcca ccacgcgatc cgcacggtga aattacagct cacagactgc ggtgaaaccc
     3481 ggggacgctg ggtgattgaa ggcgacctgt ccagttactt cgacaccgta catcatcgac
     3541 tgctgatgaa ggctgtacgc cgcaggatca gtgacgcacg tttcatgact ctgctgtgga
     3601 aaaccatcaa ggcgggacat atcgatgtcg gtctctttcg ggcggccagt gaaggtgtgc
     3661 cacagggcgg tgttatatcg ccgctattat cgaacatcat gctgaatgag ttcgatcaat
     3721 acctgcatga gcgctacctg agcgggaaag ccagaaaaga tcggtggtac tggaataaca
     3781 gtatccaacg gggccgaagt acggcggtca gagaaaactg gcagtggaaa cccgcggtgg
     3841 cgtactgccg ctatgccgat gattttgtcc tcatcgtcaa aggcaccaaa gcacaggcgg
     3901 aagccatcag ggaggagtgt cggggtgtgc tcgaaggcag tctgaaactc aggctgaaca
     3961 tggataagac taaaatcacc catgttaatg acggctttat ctttctgggg cacaggatca
     4021 ttcgcaaacg cagtcgttat ggcgagatgc gagtggtctc aacgatcccg caggagaaag
     4081 ccagaaactt cgccgcatcg ctgacagcac tgttatcagg caactacagt gaaagcaaag
     4141 tcgatatggc tgaacaactc aaccgaaaac tgaaaggctg ggccatgttc tatcagttcg
     4201 ttgattttaa ggccaaagtc ttcagttata tcgaccgtgt cgtgttctgg aagctggctc
     4261 actggctggc ccgcaaatac cgtacaggta tcgcttccct gatgaggtgg tggtgtaaat
     4321 caccggaacc gggtcagagc aaaacgtggg ttttatttgg taaaaccaat cacggcaagc
     4381 tcagcggcga aatactgtac cggttggtgg ggcaaggcaa gaagctgttc cgctggcggc
     4441 tacccgaagg taatccctat ctgaggacgg agaccagaaa cacgtataca tcgcgcttta
     4501 cagaagtggc aatggcgttc gccagcattt aaatggagag ccggatgcgc tgaaaggtgc
     4561 acgtccggtt cggggaggag aggcagggaa atagtccgac tacgccctgc ctcttactct
     4621 actctggacg ggtaagcgct gggcgtacct cgccgttgtt ctcgacctgt tcgcaagaaa
     4681 accagtgggc tgggccatgt cgttctcgcc ggacagcagg cttaccatga aagcactgga
     4741 aatggcatgg gaaacccgtg gtaagcccgt cggggtgatg ttccaagcga tcaaggcagt
     4801 cattatacga gcaggcagtt ccggcagtta ctgtggcgat accagatcag acagagtatg
     4861 agccggcgtg gaaactgctg ggataacagc ccaatggagc gcttcttcag gagtctgaag
     4921 aactaatggg tgccagcgac gggctatgta agcttcagcg atgcagctca cgcaataacg
     4981 gactatatcg ttggatatta cagcgcacta agaccgcacg aatataatgg tgggttacca
     5041 ccaaacgaat cagaaaaccg atactggaaa aactctaacg cggaggccag ttttagttga
     5101 ccacaacaga ctacctgaag ggagccgcgg tcgcctagca gttgcagtag caggagatca
     5161 tccagccgca gtacaggtca cgatgactct ggttaatgat accggctttg accccgtatt
     5221 ttccggctct atcgctgaat catggcgtca gcagccgtgc acaccatcct attgttgtga
     5281 ctgggaggct gccaccatgc ttcgcgcttt ccctctggcg aaaaagggag aaggacgggc
     5341 ccgtctgcct tcactttatg ccagcttcgg taagctgggt gagacaccga ctcatgaaga
     5401 tatcattgat aacaatcgat ccatcaactg gcctgtataa cgtggctgcc ggtgattaag
     5461 aaagctgcac ctacctaagt agtagcaaac gcacactttt tagaaaaatc gatggtcaga
     5521 aactggatta gcaattccgt tccagggttg cttttgattt acgttggcgt ctgatcattg
     5581 atttatcctc aaaagcccaa cctcattggt aatgaaccag ctccgtgaat gtccgctctg
     5641 gcacagagcg aaattttttg atctcccccc ctgaaatcta aacttagtca tgtcacgttt
     5701 ttgggtttct aaaattttaa cttcgcgttt ttcgttgccg taagggttat acagaaatgt
     5761 ccgttaagca gagttcaaaa ttgattgccg tgatcacgac tggtttgaaa gccgcgccca
     5821 agcctgtaca gctctggttt gcgttgatta tgaacctgtc agcctaaagc aagcggatgg
     5881 acgatgagta ttggtaatct ttcagagtcc ggaaaagttc agccccagtc tgaacaggct
     5941 tgctggcgcc agtccagttt cattcagtcg tggtttggtt cttacggcct gtgcaatcta
     6001 cctcattagg cacatcggcc tgccagatac cggctcgggg tgtatttccg cttccacgct
     6061 gaatactgtt ctcagcaatc ctggggtcat cacctcttct ggtgtgcctt gcgccataac
     6121 atgtccgttt gccattacca ccagttgatc gcagtaccgg ctagcctgat taaggtcgtg
     6181 cagcacagcg accaccgttt tcccctgagt ccggagttcg cccatcaacc gcatcaggtc
     6241 cacctggtga ttgatatcaa gataggtggt tggctcatca agtaatacaa cgggcgtatt
     6301 ctgggccagg accatcgcca gaaatgcgcg ctggcgctga ccgccggaaa gctcggttaa
     6361 ccgacgaacg gcaagatgat tgatccgggt ctggttcatg gcgacattaa ctcgtgcatt
     6421 gtcttcagcg gagagacgcc cccagagtga cagccaggga ttacgaccat acgaaaccag
     6481 ctcctggact gtgatcccct ctggcgttaa atggtgctga ggcagcagcg aaagcctgcg
     6541 ggccaactgg cgtgatgaga gcatatttat gggattatcg ccgagaaata cggtgccaga
     6601 ctgcggcatt aaaagccgcg aaaaacagtt taacagcgtc gatttcccgc aaccgttagg
     6661 gccgatcagg gcggtgatct tccccgttgg cagtgagagt gaaacgtcgt taagtacctt
     6721 gtctgtcccg taactgaccg tcagattttc agttcgtaaa gtcatttatc gcattctcac
     6781 aagcaaccag acaaaccacg gcgcaccgat aatggcggtc agcacgccaa ccgggagctc
     6841 cagtggggga tgaataattc tcgccagcag atcggcaacc accaacagca acgcacctgt
     6901 cagggctgaa acaggcagca gtctgcggtg acgtccaccg gtgatgctac gcatcatatg
     6961 cggcaccacg agaccaataa agctaatcgg gccgcaggcg gccacgccgg tagatgtcat
     7021 ggcgacagct agtaacaaag cccagaatcg ggtatggggc accgacacac cgagcgtggt
     7081 ggcgcgcgca tcgccgagtg caaggaggtc gagatcgcgg caaaaactca ggctcagcgg
     7141 cagaaataaa atcatcagcg ggatggcaat cttcacaaag ctccagtcac ggccccataa
     7201 gctgccggtc agccacagca gggcgttgtt cacatcctgc gggcgcgaga gcatcagata
     7261 atccgtcagg ctggcccagc atgcagaaag cgccacgccg gtgagcgcca gcttcatcgg
     7321 ctggtgggtc tttgccagca tcttcagtaa tatcaacccc gccatgccgc ccgcaaaggc
     7381 cagcagcggc agcaccatca cgggcagtga cggcataaga agtagagccc ccacagaggc
     7441 caggctggcg gcatggttaa cgccgagaat atccggtgat gccagagggt tgcgcacaat
     7501 cccctgtatc agcacgcccg ccacggcgag ggctgcaccg acaaacagtg ccagcagcaa
     7561 gcgcggcagt cggtactcca tcaatacata ataatgctcg cgtccggcct gccagtcggt
     7621 cagcagcgcg cgccacggca cggggatcac tcccatatgg agtgataaca gcgcacagcc
     7681 cgccagggca agggtgatga aaataaccag cgcaattttc atcctcgcct cctcacaagc
     7741 cagacaaagc aagggctgcc aatcagcgcc agcactgcgc ctgcgggcag atctccgggg
     7801 aaggccagcg cgcgtgcgag tacatctgcc agcagcatca gcgtggcccc cagcagcatg
     7861 ctcactggca gtacgttgcg ctgatcgaag cctgcccaga agcgcgccag atgtggcacc
     7921 agcagaccga taaacgccac cggacctgcc acgctgacgc acgcaccaac cagaagcagc
     7981 actaacatat tgatgaccaa acgtagcctc gtcaggttca ctcccagcgt atgggcggtg
     8041 ctgtcgctga ggttgagcag gttcagttga ttcgccagca gtaacacgac agggactgca
     8101 gtgaccacca ccggcaagag ctgccagaca tcctgccagc gggcgtggga cactccgcct
     8161 gccagccagt aaaagatgcc gtaagcatga tcttcggcca gcagcagggt gatgcgggta
     8221 aggcccatac aaaaggccga cagcgcgata cccgcgagga tcagtttgtt tctgtcatgg
     8281 gtatgacgaa atccgcctcc tgcggtcatg accagcagcc agctcacgcc gcccccgcat
     8341 gccgcgatga acgacagaga atagcctgca atcggcgtcg gactcagcgc gctggtaagc
     8401 gccatagcca gcgccgcgcc gctgttaatg ccgagcagtg aaggagaggc cattgggttg
     8461 tgggtcaggg tttgcagcag cgtgcccgcg agcgccaggc ttgcgccgat cagaacggcg
     8521 accaggcttc gtggcaaacg aaggttttgc accagcgctt ctggtagcgt tggcgtgtgt
     8581 ccaggcagca gggcgcgggt tgcatctgct ccggaaacag gaatggccga gtagcaaaac
     8641 agactcagcc agaaaataat aataagtgct gcaacgggaa gcccccacag cagcatcggg
     8701 tgttttatcg cggtcatttc acaacggtaa gcggctgatg gtggaagatt tttaccgtgt
     8761 cagcggcaat acgctctgca gcaaaaatac cgcgcatccg cgcccaggtg ttactgtcga
     8821 ccgaagcaac ctgctgcttc tgcgcggcgg ttaacatctg ccagagcgga tcttgttgcc
     8881 agcgtttaac aatgctctct tcgcgatagt gggcaaccag cagccaggca ggattgaccg
     8941 ccagcagttg ctccaggccg atggacggca tggacgaacc cgccatcgca gcgggaacgt
     9001 tcagccccag agaggccagc acgctgccgg tccaggtctc ctgagtatgc aggttgaatt
     9061 gctgttcgcg tgatgtgcca aaggccacgc gtgtcccttt gggaagctgg ctggcccact
     9121 gcgccatcct ctctttatgt tgttccagac gtgcctgcat ctctcgcttt ttacccacca
     9181 tttcgccgat gatagccgca gattgcaaat tttcagcgta ggtttcgttg cgggacttaa
     9241 gcagcagtac cggcgcgatt tgctgcaagg cgatgtaaac ccccgcatgg cgactgctgt
     9301 cggcaatgat caggtctggt ttcagagcgg caatggcttc caggctcggc tgcgcgcgcg
     9361 ttccgacgga ctgccacggt ttcaggtgcg cacgcacttc gggcaggatg cgttttgcat
     9421 cgttatcgtc ggcaataccg atcgggctga cgtccacggc ggccagcgca tcggcgaacg
     9481 agagttccag caccacaatc cgttgtggcg ttttttcgag tgtaaacgtg ccgtgttcgt
     9541 cctgaaccat ggcggcaaag gcgtggctga tcaccagcag caggcctgca aaaagaaaac
     9601 ggataaatgc caacataatc acattccagc taaaagcccg gcaagccggg cgttaacaca
     9661 tcagaacttc aacgacccct gcatatacag cgtgcgcggc tggcctgcat agatgccttt
     9721 gttgttgtcg tcataagagc ggatgaagta gtcctggtcg aagatgtttt tcacaccgaa
     9781 tgccaggttc agatctgcca tctgcgggcc aaagtcatac gccacgcgtg caccccagag
     9841 catgaagccg ggaatgcggc cggtactgcc gtcggcgctc tctttcaccg tattggcgtt
     9901 atccgcaaac tggctggact ggaaatcgct gttcagattg aacgtccagt ttcctggctt
     9961 gtagtccacg cccagcgtgc ctttatgttt cggggagaat ggtaccagat tgccgtaggt
    10021 gtcgcctttc tcgcggattt ccgcgttcac atacgcatag ctggcgtaga tggaaacgtt
    10081 atcaagcgtt ggcgttagcg tacccagatc gtaacgtgcc tgcgtttcca gcccggtatg
    10141 gcgcgttttg ccacgtgcag tgacggtgtc gttggtctgg ttggagtcgt actgattgtt
    10201 aaagttaatc aggaacagcc ccatttccgc cgtcagcgcg ccgtcgtcgt agcgggtacc
    10261 gagttcccag gttcgcgctt tttccggttc cacattgccg ctttgcacag ccttgccaat
    10321 ctggctgtac tgtacggtgc cgaacgagcc ttcagtgttt gcataaagat tccagctgtc
    10381 agtcaggtga tagagcacgt tcaacgccgg aagcggtgcg ttatagctca cttcttcgtg
    10441 cgtgcctgtg atggcgttgt tctggtatga ctcgatatgt tcgaaacgca tacccggcgt
    10501 gatggtccag ttgccgatgt cgattttgtc atccagatac caggcgtgcg cctcggtgcc
    10561 ggaacgcgta tcgcggtcgt aagggcttga gccggacggc aactgcccgc tgctggtggc
    10621 ggtgtagtaa cgcatttcat gcgttgattc attcacatag cgatagccca cgcccacttc
    10681 gtgcgcggaa gggccgatca taaagatctg gctgtagcgt ggctcaatac cgcgcaccca
    10741 gtagttacgc ggcgagaggg tgatgcgttt gccttgctcc aggtagccgc tgcgcagggt
    10801 ttgggtgtag aacccctgaa tgttgaattt atgctggctg tctggctgga actggtagcc
    10861 caggctcgcc agcttgcgac gaccccagaa gcggtcatac gggcgggtgg attgccagcg
    10921 atcggcgtcg taatccgcgc gagacaggcc accgggcatg tcggcttcac cgtcgtaata
    10981 ttgcagcagg ctgttgaagg tgtgcacctc atccggcgca tatttgcttt tcagcatcag
    11041 gtcgtcgatg cgggtggcgc tgtgctcgcg ccagtcactg ccgcgcgtgc cggagtagag
    11101 cagcgcggtg ccaaaaccgt tgtccgctgt gccgcccacc atcaggttgt gcgtctcttt
    11161 cgggttgttt tgtgaagagg ttgggctgag ctgaccttcc acgcccgcct cgataccaaa
    11221 gtcctgcgga atggcacggg taacaaagtt caccacgccg cccacgctct gcggtccgta
    11281 acgcaccgca ccaccaccgc gtaccacgtc aatggcatcc atgttgccga gcgaaatggg
    11341 agccagtgaa agctgcggct gaccgtaagg ggcgaagggg acggggatgc cgtccatcag
    11401 gacggtcgag cggctggcga ggcgcgggtt caggccccgg atgccaaagt tcatcgccag
    11461 gtcgtggctg ccggtgccgt tgttttccgg cgcgctgacg ccagggatgc ggttaagtac
    11521 ctcacgcatg gtggttgcgc cggttttggc gaaatcctca cggcggatca cgtcacgcgc
    11581 gccagcatgt tcaaatacgt cgttttcacg cgcatcaccc agccagtcgc cgaccacggt
    11641 cagggcatct tcttttggtg cgggcgcggg ctccagcgtc cagctgttat ttcccagcgg
    11701 ttttacctgc agtccgctgc cgtccagcag ttgttgcagg ccgctctcga cgtcgtaatc
    11761 gccgtgcagg ccgttgctct gcttgccgcg cgtcaggctg gcgtcaaccg agagggtaaa
    11821 tccgctgtgt gcggcatact gattgagcgc tttatcgagc gatcccggtg cgatattaac
    11881 ctgtgcagca aaagcggaaa acgagagacc ggccagcggc agcaggctca ggcgaatggc
    11941 gttaaccaaa ggtgttgttt tacgaaaaac gcgtaacggc gtcatacctt ccccatcatc
    12001 atttttgttg tgttcagcta tgagtcgaac gagaagggta aaaaggacaa tcgaaataag
    12061 aattattttc cttacagtgg tgaaatgttt atccagtacc gcgtaataga ctgaatttta
    12121 acgggaagcg tttgcgcgat aacgttcagg atcgcatcgg tatttttcag cgggaacgtc
    12181 ccgctcaggc gcagcccggc aacggcggga tcgcagcgca gcacgccgtt gcggtaacgg
    12241 gttagcgtgg ctatcacctc acccagcggt ttatcgctga agctcaggat gtccttcgtc
    12301 cagcttgtac tctcgtcatc cagcggtttc actgcgccaa actcagaggc gctgaactgc
    12361 aggctttcac cagcgttcac gatgcgtttt tgcgcggggg cactggcgag aagcacttcc
    12421 acagcgtgct gctgaacgtc aagctgcgtg aaattatcct gctggcggac ggtaaattct
    12481 gtccctaaag cagtgagctg gccctgacgg gtcaggacac ggaaggggcg ttgcagggca
    12541 tctttcgcgg tagtaatggc gatttcaccg taccagagcc ggacggtgcg ctgatgcgca
    12601 tcaaaacgca catccgccgc gctttgggta ttcagcgtga gcagggagcc atcttccagt
    12661 tgctgacggc tgactgtgcc tttggcggtg cggtaatctg cccgcagacc ttcgccggtt
    12721 tccgactgcc agagctgcca gcctccgcca gcgccgagca acagcagtaa tcctttcatc
    12781 acgtgacggc gggtgaggcg ggtatcgtgc aacgcccggc tggcaacgtc gccaggcaca
    12841 ccgccaagct ggttgcgcag gttttcaacc tgctgccagg cccactggtt atcctgatcc
    12901 tgttcatacc actgttgcca gcgcgcttcc tgttgtgggc tgacgcgctc gccgcttagc
    12961 acggcatacc agtgggaagc tgaacgcagc gcctgacggc gggaatcggt taacaaagga
    13021 ttcataaccc atactccaga cggaacagca ggcagtgctc gacggctttc gccacgtatt
    13081 ttttcacgga gctgatggaa acaccgagtt tgtgcgcaat ctcgctgtat gtcagaccat
    13141 ccagttgcga aagcagaaac gcttcacgtg ttttgccgtt tagcccgtcc agcatgctgt
    13201 cgaggagttg tagggtctcg agttggcttt cgcgttcctc aggtgaaggc gctcccccct
    13261 ccggcataag cgccagcatc tccagatacg ctttttccag cgcgtttcgg cgaaacaggt
    13321 ccaccatcac gcgtttggcg atagtgcaga ggaaggagcg aggatcgcgg atcgtcgaga
    13381 gcgtttcgct gaccattacc cgcaaaaaag tgtcctgggc aatgtcatct gcatcaaaag
    13441 cagactggag tttgcgcgtc agccagcttt tcaaccagcc gtgatgtgtg ccataaagcg
    13501 actcgaacgt taaggaagct gtggtagtgg cgcggtcaga catgcggagt gcatcaaaag
    13561 ttaattatca cgtagtcata ttaatatgag aatggttatc attacaattg gaaataaaat
    13621 tgtttccaat agacattttt aacatgttgt ttttctaagt gttataaggt aggtataaaa
    13681 tgggatggag cctctgcttc tgacatgtgt cggtcagaat gactcatgat gtggtctgct
    13741 attattgaca tcctcactgc cctaaaggat ggggatttcg gtaatgatct tacccagcaa
    13801 tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca agacaaaaca
    13861 gtttcaacca gtaaaaaaac gtaaacacca ttcgcatgaa tttcgtagtt aagatctgaa
    13921 gcttgcagaa tgcatcggtg ttactgccgg agtccgtgaa cttagtctgc atgaatcacc
    13981 acagctttac atctgtcgca gtaaacagca aaatcagcaa acttacttaa ttatatcgag
    14041 tgcgattaca atcgctggcg atggtacagt tggtgtggcg gtctcagtcc ggaacaattt
    14101 gaaaatcata atctcactta aaacggcatc cattttacaa aagatggccg tctgtgtatt
    14161 tgtaatgaat accttctaca tgtttagagt gcataatcca cgacaaggcg atatcctaaa
    14221 aattagctag caggcaactt tgatggcata gataatgttt ttagtaaaaa agagttgact
    14281 gaatataata cagcctcttc ctacataaga aggaatgtgc tcacaaaaaa aaaccattag
    14341 tctgcttcct gttcttacaa taatcagtat tatgtatttt atttatatag caaaatatgc
    14401 ttgttacacc tgtcataatt aggcatggta aaattattga ctgaaccctg ggggagaaag
    14461 atgcacagtg attattataa tatggttttt ggtgaaaaat tggccaatat tttatatgaa
    14521 gctaactctc aattttttta cgagagaaat gtcatagaag aggcagtcaa cgctttattc
    14581 tgtgagcgtg atataataaa caacaagaac ataataaaga agctgatgtt ctttttgtca
    14641 gatgttaatc acaccaagaa agatgttgtt cagagcgcac ttaatattat cattgacatt
    14701 acatcaggtg atatttgaga ttttacataa aagataatct ctcaaggagt cttatctgaa
    14761 aacgaaatat atagtccctc tcgttttgta atatcgattg gctttgttga atcggttacg
    14821 taccccggag ccggtcatat tttcaggtaa tacacacgct ttcagacatt tcggctttcg
    14881 tcatttttgt atcatgggca tagcttctgc tacctgacca tcataatcac gcagcttcag
    14941 tgaagcagtg accacctgtt taacccggtg tatcgttatg atcttatcca gcaattgaac
    15001 cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga tcatcgtttc
    15061 cgatggaagc ataataagct ttttctgctt ctgccggtgg gatatggccc tgcctttcca
    15121 gcaatcgtcg attgttatac cagtccaccc acgtgagtgt ggtcagctca tcatactgac
    15181 acgtgcatta cccctaccgc gataatgcaa ccgtacaata tagggcgttt cctaaacgca
    15241 gccctcatca ctccaatcca acaagacaca gcatttttga tcacgaagaa agacaaataa
    15301 tttccgtaaa tgtttccgtt gttacaaata acaacagtgt tttacatttc tttggcctca
    15361 ggcccgcgca tgttgccatt cgatgtcatt ttcctgactc gtttcttctt tatggtgtag
    15421 tgtgatcttc actttataga tgctaacaat atgaaaagaa aaattattcc cgtgctaatc
    15481 ggttgtacgc tctctttctc tgccctggcg gcacaaccta cggctgagcg ctatgtcgtc
    15541 agttttccag aaggcacgca cgtgaactac gccggcgcat ttgccagtgc attcccgaac
    15601 ggccttccgg ttgggatagg ttctggcctg ctgttcaccg gtaaacaggg cgatgccctg
    15661 acattcgcaa ccattaccga tcgcggtccg aacgcggatt caccgaaaga ggggaaaaac
    15721 gaaactaaaa ttttcgttac gccagatttc gcgccgctgc tgatgacgat tcgtgtacaa
    15781 aacggcaaag cggaggccat cgatccccgt cctctgcatg acgataaagg cgcgatcaac
    15841 ggtctgccgc tggcaagcga cgtgattggc tccaccaatg aagtggcctt cagcgacacg
    15901 ttacatagac tgaaggggga taatcgcggg ctggatacgg aagggatcac tccggatggt
    15961 aaaggaggtt actggctgtg cgatgagtat ggcccgttcc tgattaacat cgacagtaaa
    16021 gggaaaatcc tggcaatcca cggtccgcag gcggcggaag gggaaaaagc gattgcgggc
    16081 ggtttgccaa atatcctcaa atggcgtcag gcgaaccgcg gctttgaagg cctcacccgt
    16141 atgccggacg ggcgtattat tgtcgccgtg caaagtacgc tggatatcga cgctaagagc
    16201 aagaaaaagg cgctgtttac gcgtctggtg agcttcgatc cggcaagcgg aaaaaccgcg
    16261 atgtacggtt atcctatcga cagcgcggcc tacagcaaaa acagcgacgc taaaatcgga
    16321 gacatcgtgg cgctcgataa ccagcacatc ctgctgatcg aacagggaag agataaaaac
    16381 aacagaatgc gtaacctgat ttacgaggtc gatctcaata aggcgagcga cctgtcaggc
    16441 ttcgacaagc cgggtgagta tccggagttt gatgatgaga aaaccctgtc acagcgcggc
    16501 attacccttg cgcagaaaac gcaggttgtt gatctgcgct ccttgggctg gcagcaggag
    16561 aaggctgaag ggttggcgct gatcgacagc aaaacgctgg cggtggcgaa cgataacgac
    16621 tttggcgtga aggtggcgat gcaacatccg gtcgagggca agacattcaa ggattatcgg
    16681 gtgaacgcgg aaggaaagct gacgcttgac gacaagcagg ttgaaacgac cctcagagtg
    16741 aagccactgg agaaaccgga gtctgacagt gaactgtgga ttgtgacgct gccggaagcg
    16801 ttgaaataag tttataaggt acggacagca gtatcgctta gtttttcaaa ttcatgtatg
    16861 tgaacaatat tggggttccg gcgtagaccc ccaatgaaag cgtcaccacg tgaaaacatg
    16921 cctgcacggt ttacacgtca tcccatggtg gcagccggtt aactttcctg cgccaccagt
    16981 ggaaaaaggc cataagcagt ccgaacaaaa gtaagcgtac agcgagggcc gtattgacgg
    17041 ggatgtgtta ttcagctggc agtgctatgc gccacggaag cagttcgctg acccggttga
    17101 ccggccagtc tgctatgacg ccaagcacat ggcgaaggta gctttctgga tccacgtcat
    17161 tcagtttgca cgtcccgatc aggctgtaca gtagcgctcc ccgctcacca ccatggccag
    17221 agccgaagaa cagggtcttc atcttttcac gcaaccagct ttccagggat ttcaacagcg
    17281 gtttcgtttt tcgctgacgt tcagcaagcc gctgctctgc cggcattccc cttatatccg
    17341 cctctatggc gtacaactga ccgatctgct ccagggcttc ttccgtcagt gctgacggga
    17401 tgcggacgtg cacatcgtgg atctttcggc gggcatgagc ccagcaggca gcttccgtta
    17461 tcccaccatt gcgatacagc tcgttgaacc cggcgtacgc atccgcttgc agcacaccgc
    17521 tgaagcaagc aagatgagtc tgcggatgga tgctttttct gtccgggctg taagcgaacc
    17581 acactgcagg tgccaacgct gacccggcat tgcggtcatc acgaacatac gcccacaacc
    17641 gcccggtctt cgtcttctta ttacccggca gcagtacctg gaccggggta tcatcggcat
    17701 ggagtttgcc gtcagtcatg acatagccat gaagcgcctc ttccagcgga gacagcagcc
    17761 ggcagcatgc atccacccag cccgacagca gtgaacggct cagctccaca ccttgccggc
    17821 cgtatatttc tgactggcaa tacagcgggg tgtgctctgc atacttcgag gtcagcacgc
    17881 gggccagcag ccccggtccg gcgatacccc gctcgatggg ccgcgaaggt gcaggtgcct
    17941 gcacgatggt atcgcactga gtacaggcat gtttttcccg taccgtccgg ataacccgga
    18001 aggcgctacg catcaactcc agctgttcgg cggtatcctc gcccagatag ctcagtgaac
    18061 cgccgcagtt cgggcagcac ggcgccgcag gcaacagtcg cttttcgtca cggggtagtg
    18121 attcagggaa cggcttacgg gtgcgggtct gacgcaacgg acgctgtact gccgggtcat
    18181 acaccctacc agtcagcgta tcgctctctt tctgaagccg gttcagatcg gcttccattt
    18241 gtgcgatacg gcgggagact ttttcggaac gactgccgaa gttcatccgg cggagtttat
    18301 ccagctgcgc ctgcagatgg tctatttcgc gctcccggtt gctcagcttt tcctgcaggg
    18361 cgtggatcag cgcttcctgt tcggccaggc gctgtttcag caggaagatg tcgtcagaag
    18421 agatgtcgtt cataagcccg tattttaccg ggcttattct gtgacaacca ggataaagag
    18481 atttacagca tggtcaggga ggtcagcagc cgcttgggct gtcgccagtc gataccttcc
    18541 agcagcatcg ccagctgcgc ctgcgtaagg aacactttgc catcacgggc tgacggccag
    18601 gcgaagcgcc cacgctccag ccgtttggtc aggaggcaca gtccgtcgcc ggtggaccac
    18661 agcagtttaa cctgactgcc gctgcggccc cggaaaatga aaatatggcc ggacatggga
    18721 tcgtctttca gcgccgtttg tactttcgca gccaggccgt tgaagctatt tctcatatcg
    18781 gtgataccgg caaccagcca aattttggtc ccggaaggta acgggatcat cgcttcagtt
    18841 cctgtatcag cagagtcagg agcttttcgc tgacattgcc attgaagcgg agcgtcccgt
    18901 gccggaacgt tacctcacag ctgatactga gggtttccgg gtcctctgcg agcgattctg
    18961 gctgttcggc agctgcatcg agagtcacag gaagtagctg ggggctctct gaagaaggta
    19021 atagcagctt tccctcgcgc cattgttgtc gccatttgaa caacagattg gcgttaatgc
    19081 cattttcaag agcaagtttt gagatggata tcccgggttc acaggaggca gcaacgagca
    19141 gctgtttaaa ttcgggagga taattagggc agccttttcg cctgccggga gtcacatttt
    19201 tctgcatatc tgacactttg gttcccacta cttatttggt ggacaccact ttgtctaatt
    19261 cgtcagattc tgaccagacg gttcaggctg tacgcttacc actattttat ccctgttgaa
    19321 tttggttaag ccggtgcaga cgcgccggga aacgcacacc tgaaccggca caataaaata
    19381 caatacaacc ccctgttact ggagatacga atggaatgac gaggcagcct gtcagtcaca
    19441 gggggctgtt ggcataaaat caacggaaag gccaccaaca ccggaattaa gctccacagt
    19501 ccccgcactg gcaatccatt cccgggaaag gcatcaccgc aaactgctcc ggaggcaacc
    19561 attacagcct ctgtaacgga ttatcagccc tggcatcttc cgacggcacc tcctgccgcc
    19621 cccacgccct gctgtatacc ccggtaccgc agcgtgggca gcacttttcc ccctcataat
    19681 cttcgtcaca catcacacaa aaccactttc tcaccttcac cgtataatcc ggaaccgtca
    19741 tgccctgcag atgcaaacga cgggcctctt tctctgccgg gtcaagccag gtacatcgca
    19801 gcaggatttt ttcagcgaca gtcatctggt catgttcaaa ccacggtggt tgtccgtcat
    19861 tgccccgctg aagtgttaac gggcatccac aggacacaca gcacaaaacg tcctgtcggt
    19921 tgtgcttcgc agcttctttc gccgtaacaa aatggccttt gctgtcattc gccatataac
    19981 agttcagaat tcgcatattt cctcctgagt tgtccgggga atactgaatc ctccggtctg
    20041 gcagtgtgtg cagtactgct ccccataata atcgtggtga cattgtctgc agtgccagct
    20101 ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgtt taatcaactg
    20161 tatttctctg cgctccggcc tgacataagg gcactgttga ccgtgctctg tcagcctgtc
    20221 gtcagtgtgt tcaaaccagg gaagttcagt ttcgtattgc ggatggtatc tgagtgcact
    20281 gccacacaag tggcaggtgt agttagcata aggtgcggcc tgtgcagtac gggcgcccgt
    20341 cagacgtccg ttgccatcaa gagcgataaa ggattttgcg tacatagtca tatacctcag
    20401 ccgccagacg acacgcaggc aacagccgtc ccggtacggg cagcgtggtc agggtgtgaa
    20461 tggcggttca gttaaaatct ggactggagt ttgggtaaag caggctggtt gctgttacag
    20521 cactgtggtt gtcacacaat ggaacactga cgtaaaaaac agtcagtggg cgagtggata
    20581 catcgttgcc gccagacaac aacccatact tttggtttgc cggatattgg cgcgccagtc
    20641 gtgctcaact ccggctacag ccaaaattgc gctcaccggt gcagtgagct ttggtatgct
    20701 ccttcgccag atagtcagca cgttccagca cttgctgaaa gccggtgtca tcattacgcg
    20761 ccagccacac cgccggccag gccggaaaat gcaccagcgt ggcatggcac ccgacatcca
    20821 ctcccagggc actgcaccac gcctgtttaa tcatcccggc cagagactcc ggagcgcggt
    20881 aatcaccggc acgacaccag gtatcccggt tgaccagcag catcaggtga tagtgttttt
    20941 tacctttgca ctccccaaac tcccgggccc agacgtaatg cagggttgtg ggatgcacgc
    21001 gtttaccttc ccgatgctta cgtttctggt aagcgtcgat tcgggctttc agggcattga
    21061 tgaagcggga tatcacagct gcgtccgttg ctgccggtac atccgggaga cgcaggtcaa
    21121 cccgcaatgc cgtcaggcgg ggatgaacat tcagtgcgtg ccgcaccgtc tcacgaatac
    21181 gttgctgcca gaaggggttg tatttgtagg tcatggttaa atctccgtat ggttcatacg
    21241 gaatagccac gtcgtaaaaa atacgcagag tccctgaagt gacctccggc aggacactgt
    21301 ctcaggcact ctgtgataac ccgaacggtc gtctccggac tgacggttga attttcagcg
    21361 tcgtttttta atgtaaaaaa ccggttacgg gtaaggctgt gaggagggag tgatggtgat
    21421 acgcaaaaag aagtgcaggg actgtggaaa cgcgattact cataatacag tgtgttgccc
    21481 gtattgtggt tctgtcgacc ccttcggcta ctaccggaat acagaccgta tcgttaccat
    21541 actgctggcg ctgattattg tggtcctgct gactactgtc agcgtcagtg tgtgtatact
    21601 gtgtagctgg tgagataacg gttcatgtat cccctgagct gacagtgtac cgtgtatatt
    21661 aagcgtaacc gacaggttct ccttcatacc atattaaatt ttccagggat tgccgctgcc
    21721 ttatttcgtc ccatacagga aaactcatct gggtttctga caaatacgcg aaactcacaa
    21781 cccctcaata tcaaccacac gttgtaagcc ggtaatacct gaaaatcagc ctattaccgc
    21841 ccacgcctcc ggcaccattc ccttttgatt tcgaattctt tgttgtcgct ataccgctta
    21901 atgttcccgg tcctccggtg cagataacac cggagtcatc gtagcgctgc ttcaatattc
    21961 aggtgacgta tttccagacg gaagccggcg cgcgatcgta aagtttgttc attgtcagtt
    22021 ctgccagccg gttgtcaacg gcagagttaa aggcttccag ctccgcaagg gtaagtttat
    22081 aacgcgtgtg ggaattcact tttcccgcgt ctcctgacgt attacgggct gccacttacc
    22141 tggtgtcccg caatacagcc ttagtgttcc gttatcgggg atccggacgc accgcaacaa
    22201 ccggggaagt atttctggtt tagagcagtg tcaagaggga tttctcagcc cctcactcac
    22261 cggctcagtc tcagggcagg agcattaata cgctcagttc atgtcttata caggaatggg
    22321 cacaggggga gctggcgtgt cactgctgat tttacgcatc agcgttcatg tatctgcact
    22381 ctgatacatg aacgattcac agcgctctgt taccggagag atttactctc ttagcttact
    22441 ctggcaaatc ctttacgtta ctctctgatg acttcttata tttataatac caatcagaca
    22501 gccttatccc atcg
//
LOCUS       DAEEDS010000061        22160 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEEDS010000061 DAEEDS010000000
VERSION     DAEEDS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.061"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KLH32_002654"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KLH32_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBE0139423.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="KLH32_002655"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="KLH32_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBE0139424.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="KLH32_002656"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="KLH32_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBE0139425.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KLH32_002657"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KLH32_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBE0139426.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="KLH32_002658"
     CDS             3251..5011
                     /locus_tag="KLH32_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBE0139427.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="KLH32_002659"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="KLH32_002659"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBE0139428.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KLH32_002660"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KLH32_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBE0139429.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KLH32_002661"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KLH32_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBE0139430.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KLH32_002662"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KLH32_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBE0139431.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KLH32_002663"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KLH32_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBE0139432.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="KLH32_002664"
     CDS             complement(9679..11586)
                     /locus_tag="KLH32_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0139433.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KLH32_002665"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KLH32_002665"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBE0139434.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="KLH32_002666"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="KLH32_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBE0139435.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KLH32_002667"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KLH32_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBE0139436.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMLLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KLH32_002668"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KLH32_002668"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBE0139437.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17633)
                     /locus_tag="KLH32_002669"
     CDS             complement(16893..17633)
                     /locus_tag="KLH32_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBE0139438.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGGEASTVPALLLAAPPLILLKPG
                     TTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLP
                     GDPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWC
                     QVIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            complement(17599..18114)
                     /locus_tag="KLH32_002670"
     CDS             complement(17599..18114)
                     /locus_tag="KLH32_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0139439.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18122..18664)
                     /locus_tag="KLH32_002671"
     CDS             complement(18122..18664)
                     /locus_tag="KLH32_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0139440.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18762..19745)
                     /locus_tag="KLH32_002672"
     CDS             complement(18762..19745)
                     /locus_tag="KLH32_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0139441.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19736..22108)
                     /locus_tag="KLH32_002673"
                     /pseudo
     CDS             complement(19736..22108)
                     /locus_tag="KLH32_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT         5495 a         5804 c         5204 g         5657 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgaact ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac agcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc cccccccccg
    17401 gcccaacgcg ttgggcgatt gattttgctg ttaataagcc aagattcctg actggtatta
    17461 gtcagtaaaa tagatatcga ttctctgtct gccggaaaga taaatcgcgt tccgccgacc
    17521 accactcctg cctgcaattc acccgccacc agcagcgata aaacggcgaa aatcaagagc
    17581 gctaatctgt tccacgtatt agtcataatg aagtacaaat gtcgcgctgg cattcacttt
    17641 acccgactgc acatcgctat tgacaggtcg caattgcgca taaaaagtta atggcacctt
    17701 accgctatgt aggctgtagt ctttactcgc ctggttaagc ggaatcaata tcttcgcatc
    17761 atccagaatc gcaattgcca gcccactggc tgcatccgta ccactttcaa tggacagcat
    17821 ttcgctctgc actccgtcgg gggttcctga aaaggtgaca tcaaccgtgc tgacatctgt
    17881 tccgcaatcc tgcaaattaa tgatgaaaga tttctccggg ccataattcc catgaaaaaa
    17941 acgtgtcagc gagacatcgc ctaactgcac attgatttgc ttgctatcct gtgccagcac
    18001 gcatgtatta cggcgaaacg atccgctaat gttgaccgtg ccatcatggg caaatagctc
    18061 acagcccccc gttaatccca gtaagaacag aatccagatt atccgtttca acatcactcc
    18121 ttcactggta aaccagatca aaatagagaa ccgccgtcgc attaccgccc gttgctcccg
    18181 cctttgtgga cttataacga agttgatatt tgagtgtgtt atccccggct tttaagggcg
    18241 taagaggctg gacctgattg agcgggattt cttgctgact ttgcgcatca agaatttgca
    18301 cggcaatccc cgttgccata ccaccgcttc cgcctgtgtc agttagtttg agcagtgtcg
    18361 gcgcaagggt gtcagcctcg ccgctaaaaa ggacgtccgc gccagtaata ccagcggcac
    18421 aaccggtgat atcaatattc aaatcagcag cagtgcttac ggtattcgcg gcctgaaaat
    18481 cactgatatt aaaataacca atatgaatat tttgtaatgc acttttggtg gcgacatcac
    18541 agccctgatc gaccacattc ccataaatat gcatactgac ttgttcaggc ccagccatcc
    18601 cgacatggat gttaagaagg ctaaagaaca aaataacgca ctgatatatc gtttttttct
    18661 tcatacgttc acgcgttaat caaaatttat tctcaccgtt gccgtggctt caaatcgccc
    18721 ggtttctggt ggtacgcctg tcgtgctgac tggatagaca ttcattcgcg gatactgtct
    18781 gcatcatcag gaatagagag cggtagtgtc ccaccagcag gggaaataat gttgttctgg
    18841 ctatcggtca ccaccacgcc cacgccagga cgtgacgtct taatcgcctg gggatagctg
    18901 gggtctgagg ttgccgtcag actcagattg acgttaacta accctgcgtt tcccgtgtta
    18961 cacctgaccg gaattgcaag ttctgccgtt ctggccccaa gaggcttatg ccctacaaca
    19021 cgaaagttag ccgcaaatac gtcgcccaga tcaacctcga ttgtttctcc ggcattgacg
    19081 gaacatgagc caagtgaatt gatcgtacca gacaggtaat acactaaaac cggtgtacca
    19141 gtagtcgtgc aggaatcacc tgcgggtatg ttgtagcatt caaataatcg cgccagcgcc
    19201 ataggaggaa tgacgaccga accaacgaac ggctgcaata ttttcaggga aaagctaccg
    19261 ctattaccgc tagccccctg gccacctaaa cgtagaccgc cgggatcgca aatatgccat
    19321 cgtgtatctg cccctgtgcc cacgttttcg aaaggaaccg cagtaggaga actgccttta
    19381 acctgcaata aaactttggc ggcaagatag tcattaattt tgtaccagtt gtcgcctaaa
    19441 tagactaacg gtgaatccgc ggcgaatgcc catcggccac ttctgacatt gtctttatcg
    19501 caatcacagg aaacataata atcattcgat tcgttccact gataaaattt gtctttaact
    19561 actccagccg tattttcttc ttcatccaga gttttaacgc caaaattgac gccaatatac
    19621 ggattcccct gagtggtatg acactgcccg atataagcag cagtttgagc gcaccatagc
    19681 attaagacgg cggcaaaaaa tagtcgtagt aataacacgc atttttctcc aaagattatc
    19741 tgcatatggc atccattcgc gtaatcgcag agttaatttg ttgttcggta agctggtaac
    19801 tgatatgaca aatttgatcg ggagcttcgc cccagcgtac agttatcgct ccctgagcgg
    19861 gcaaaccgga catatagagc gagccatcat catcaataat gccgctatta gtctgtatgc
    19921 cgtccagcgt cgctatcgcg ccaaaacgca gcggtatacc ctgctttgat gttttcatta
    19981 atacccttgc tcccgcatgg gcattgaact ccgccagaac caacgcccct ttggtgggaa
    20041 ccacgttgac tactgcattt tcaagatcca cgttacgttt taacgatgcc gcatcaagtg
    20101 cgattcgatt ttcccgataa gaagtggcat aggggacaac ggtatatccg cgccagtcag
    20161 ttttcagccc ggtgctgttc gccacacgag tattctctgc gcctggtgct gcaatcagca
    20221 cgttagtttc ccccagcgat tgccccaggg taatgccctg tgaatgggca actaaactgc
    20281 ctgaaagagc gtagttcagt tgttgttgac tgccgttatc gctgtagttg tagcccactc
    20341 gggcatctgc aaacgcccct ttgtagtcca tgctggcgct accattagcc gttttcccct
    20401 cgctgttata tccctgttgc acgctgtagc tcaggttacc gtcatcgagt agtgtctccg
    20461 ttaaacccag gttttgtgta atgtgccctt cgttatcgat actggtgtta gaggtcatcc
    20521 aggcgtaatt ttttttcctg gtatagctat cattacttct ggggagcaac agattgagcg
    20581 gcagggaaaa atttagtgca aacacctgat cagcatcagg ttgaccacgg gacttactgt
    20641 agttccagga aacccccagc gagagatctt taatttgcgt gttgtagcca aactgtaata
    20701 gccgatcctg ttgatcggta tgccagtaag tttgctggct accacttaaa taaaacaaac
    20761 cgtactcgcc taattgctgg gagatattga cctgcagttt gccacgtttg gtgtaaaaca
    20821 aattgtaata acgcgaccac atcggcgtat cttcatcatc accgtcatta aattcgtagc
    20881 catccatatg tttatacatg gtgtcggaaa gggtgtagaa acccgacgtc gaatagcgat
    20941 agcccatcaa ttgaaaatta gtgcccaatt ggttgagcgt tttggcatac agaaaacgat
    21001 atgactgacc tttatgttcg ctttggtctg ccagcgtact cttcgcctgg gtcgcatcga
    21061 acgaaattgc tccgaaatcg ccaaggttaa atcccagacc aaacatggcg gcacggtaat
    21121 attcagcata ttgtccacca ccataccatg tcgttcccca cggtccgccc cactgcaacg
    21181 tggtctgggc aaatttgctc tcctgctgtt cattactatt ggttctgtat ttcgccagtg
    21241 tcaccgcgta tttgattcgc ccctgacgct ggagtaatgg cacgctggaa aacggtacgc
    21301 tgtagctatt gacgctgccg tccgcttctt tgatttcaac taacaaatca ccgctcgacg
    21361 acgtggaata gagatcacta atttcaaaag caccaggcga tacataagtt tggtaaatgg
    21421 tgtaaccatt ttgcttaatc gttatctggg cattgctttt ggcgatacca cgcaccacag
    21481 gcgcaaaccc tttcaggctg tctggcagca tgttgtcatc tgacgccagc tgcacaccac
    21541 gaaagctgac actatcgaaa aaatcgcctg cagtataatc atcacctacg gtcagttcgc
    21601 cttttagtgg aataaccgcc cgctgcaaat agctgctgag attcttccat tctgcggttt
    21661 ggccactact gcgactccat gttgaattgt tgcgcaatct ccatgggcct aaattaacgc
    21721 cactgtttaa attcagaaaa tagctgtcgc cagaatcact gtctgcgctg ctatgaatac
    21781 tgttagcccc gctaaatagc gcgttgatcc cttcatccca acgttcaggg gggacataac
    21841 cacgggcatt acttttgatg gcgatttgcg gtactgaaat ttccagacgg agtttattca
    21901 cattaaacgt tacgctggca tccggaataa tctccgccaa tggcacacac acttgtttgt
    21961 ccagttgttt aaaagccgga aacgcatcag tattaacacc cattgcatcc aggctttcag
    22021 tcgtcaaaca ggcggcaatc cccttctctg gcgttatctc aacaaaattg acgttgcgcg
    22081 tatctacaat tgtctgattc agaacaatgg taatgactcc aacttactga tagtgtttta
    22141 tgttcagata atgcccgatg
//
LOCUS       DAEEDS010000062        21701 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEEDS010000062 DAEEDS010000000
VERSION     DAEEDS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21701
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.062"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            58..1059
                     /locus_tag="KLH32_002674"
     CDS             58..1059
                     /locus_tag="KLH32_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE0139442.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            complement(1350..1784)
                     /locus_tag="KLH32_002675"
     CDS             complement(1350..1784)
                     /locus_tag="KLH32_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0139443.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1756..2547)
                     /locus_tag="KLH32_002676"
     CDS             complement(1756..2547)
                     /locus_tag="KLH32_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0139444.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(2547..3227)
                     /locus_tag="KLH32_002677"
     CDS             complement(2547..3227)
                     /locus_tag="KLH32_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBE0139445.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(3224..4423)
                     /locus_tag="KLH32_002678"
     CDS             complement(3224..4423)
                     /locus_tag="KLH32_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBE0139446.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(4424..4777)
                     /locus_tag="KLH32_002679"
     CDS             complement(4424..4777)
                     /locus_tag="KLH32_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139447.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(4777..5517)
                     /locus_tag="KLH32_002680"
     CDS             complement(4777..5517)
                     /locus_tag="KLH32_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBE0139448.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            5740..6030
                     /locus_tag="KLH32_002681"
     CDS             5740..6030
                     /locus_tag="KLH32_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBE0139449.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(6054..6398)
                     /locus_tag="KLH32_002682"
     CDS             complement(6054..6398)
                     /locus_tag="KLH32_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139450.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(6411..7475)
                     /locus_tag="KLH32_002683"
     CDS             complement(6411..7475)
                     /locus_tag="KLH32_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139451.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(7478..7783)
                     /locus_tag="KLH32_002684"
     CDS             complement(7478..7783)
                     /locus_tag="KLH32_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139452.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(7785..8387)
                     /locus_tag="KLH32_002685"
     CDS             complement(7785..8387)
                     /locus_tag="KLH32_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139453.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(8387..10402)
                     /locus_tag="KLH32_002686"
     CDS             complement(8387..10402)
                     /locus_tag="KLH32_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBE0139454.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(10580..11005)
                     /locus_tag="KLH32_002687"
     CDS             complement(10580..11005)
                     /locus_tag="KLH32_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139455.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(11009..11449)
                     /locus_tag="KLH32_002688"
     CDS             complement(11009..11449)
                     /locus_tag="KLH32_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HBE0139456.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(11460..12605)
                     /locus_tag="KLH32_002689"
     CDS             complement(11460..12605)
                     /locus_tag="KLH32_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HBE0139457.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(12609..13166)
                     /locus_tag="KLH32_002690"
     CDS             complement(12609..13166)
                     /locus_tag="KLH32_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139458.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(13141..13530)
                     /locus_tag="KLH32_002691"
     CDS             complement(13141..13530)
                     /locus_tag="KLH32_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HBE0139459.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(13517..14071)
                     /locus_tag="KLH32_002692"
     CDS             complement(13517..14071)
                     /locus_tag="KLH32_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139460.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(14068..14475)
                     /locus_tag="KLH32_002693"
     CDS             complement(14068..14475)
                     /locus_tag="KLH32_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HBE0139461.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            14474..14708
                     /locus_tag="KLH32_002694"
                     /pseudo
     CDS             14474..14708
                     /locus_tag="KLH32_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14705..15646)
                     /locus_tag="KLH32_002695"
     CDS             complement(14705..15646)
                     /locus_tag="KLH32_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBE0139462.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(15658..16161)
                     /locus_tag="KLH32_002696"
     CDS             complement(15658..16161)
                     /locus_tag="KLH32_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139463.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(16166..17386)
                     /locus_tag="KLH32_002697"
     CDS             complement(16166..17386)
                     /locus_tag="KLH32_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBE0139464.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(17401..18138)
                     /locus_tag="KLH32_002698"
     CDS             complement(17401..18138)
                     /locus_tag="KLH32_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBE0139465.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(18026..19492)
                     /locus_tag="KLH32_002699"
     CDS             complement(18026..19492)
                     /locus_tag="KLH32_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBE0139466.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(19492..21114)
                     /locus_tag="KLH32_002700"
     CDS             complement(19492..21114)
                     /locus_tag="KLH32_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HBE0139467.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(21117..21689)
                     /locus_tag="KLH32_002701"
     CDS             complement(21117..21689)
                     /locus_tag="KLH32_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBE0139468.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
BASE COUNT         4927 a         5929 c         5385 g         5460 t
ORIGIN      
        1 ggactatgac ataaagatct gatattttcc tcacaaaaac ttagggattt tattttaatg
       61 cataagagca actgctttga tacggcaagg cttgtggcag ctatgatggt tctaatcagt
      121 caccactacg cgctctctgg acaactcgag ccgtatatat tcggctttga aagtgctggc
      181 ggcattgctg ttatcatatt cttttccata tcaggatacc tgatttcaaa atcagcaatc
      241 agaagtgatt cctttattga tttcatggct aaaagagcaa gaagaatatt ccctgctctt
      301 gtcccttgct ctgtactgac gtatttttta tttgggtgga tgcttaacaa cttcagtaca
      361 gaatatttta gtcatgacat tatccggaag accataagct ctattttaat gagtcaggct
      421 ccagatgccg acattacatc acatttaatt catcccggta ttaacggcag cttgtggaca
      481 ctaccactgg agtttatgtg ttacataata actggagtgg ctgttgctct actaaagagc
      541 ggaaaaacgt tcatagttat actattgatt tttgtttcac tttcattagt tgaaagtgta
      601 tctgagaaca aggatgtaat attctccatt ccagtatggc tgtatccttt gcgggggatg
      661 gctttctttt ttggtgccac tatggcgatg tatgagaaat cttggaatgt aagtaatgtg
      721 aaaatttccg tgatttcttt gcttgctatg tacgcatacg cgagctatgg caaaggcatt
      781 gactatacaa tggcttgcta tattctggtt tctttctcaa caatagctat atgcacatcc
      841 tttggagatc cactggttaa gggtaggttt gattactctt atggtgtata catttatgca
      901 ttccccgttc agcaggttat tataaataca ttgcatattg gcttttaccc ttcgatgttg
      961 ttaagtgcca ttgtcgttct tttcttggct catctttctt ggaatctaat tgaaaaaaga
     1021 ttccttacac gaagctcgcc aaaattatcc ctgaattgaa taataaaggg gccatcgagc
     1081 ccctgaataa atcaaagcca gatataacac tgcctggcag tggacaccgt tctgatgcct
     1141 cgtcctgcaa ggacgagcga aggtggagtt cttttccacg cagctattta gtacagtact
     1201 gagtcatatt gtttatgtat gagttaactt ccgtaatatc aattaaataa aataagttga
     1261 ggccattgta gaagtgagca ttatatccag gttatctgga cctgttgaaa aatgcgtatt
     1321 attccaacaa ttaccctaga ttaacaagtc tatttatttg gatgcatagg ccactcgggt
     1381 tttactacat taacacgcat aagcagtacg cgatattttt tccatgccag taacagtttc
     1441 gtttcatcat ctgttgccat acctaaatca accgcatcct gaagcggcgc tattacattc
     1501 gttgcctctg ctatcagtgc agatttcttt tcatcattta ttaaggctaa ttgttctggt
     1561 gttggagaag gacggtcaac aatgacaggg tgaccgttct caccacaggt aatcattttt
     1621 gttacactct gtgagttaat cagtgttttc cactctgatt ccgagatttc cactgcatca
     1681 tcaggaatat tggtgccgtg aaattcagtc gaataaaatc cgtttgtcga tgcagaataa
     1741 aaatatgaca tttcgttaat atcccattgc aatgtaatac gcatcagcag atgaaccata
     1801 tcctgaaccg ggcgacactg taatatcaaa tccagtgtta tggacatttg tcgccgatac
     1861 agtcggctgc cctgacgcgc cagtaacgtt caatgtaact gtgacagcaa ggactcttga
     1921 gaatgtcatc gggaacctca tcccgtatgt tgaagataca ttcggggagc cattcaactg
     1981 accgcactgt aaaattaatc cagacggaaa tttttgcgca gcagccgttg cggtattact
     2041 tgacgcgaac aggctcatat caggtatctg attcgcccct gtacctacat tccttttagc
     2101 cgcttctccc aaaccaaggt atgtgagaat gtcagcaata gtatttttcc caataatgtc
     2161 acggccaaca gaagttaaat cagtctgcgc tgctgtatca tttccagtga aatatgggag
     2221 tttatttgca cctgttgcga gcccagctaa tgccgacagc gtggcatcaa gcgcctggaa
     2281 atctttcccg aaagcggtcc ccattttgga tataaacccg ttcaggtctc catcatcaag
     2341 cacatccagc ctgcttttgt tggcggtgta ctgcgccaac gctgccgcga taaagctggc
     2401 ttgccgaata gctttgttga cctgtgcgct ggatgcttta cctgccgtaa accctgaaag
     2461 cagagccgga agtgcttccc agtcagcttg tgaggtgacg ttagcgttcg gatcaagcgc
     2521 gaaaggttta aagttgttta ttgccattag agtattgtcc cccatgctcc aacatcgaac
     2581 ccgccgatgt attcgttatc catatcaaac ccaaagaatt tagagccctc ggatggtgct
     2641 tctaccgaag gcgtttcaac atcaccggcc catacgccag ctgctttaac ggtgagatag
     2701 ccctgtttga tagcggcaat cagttcaaga gacacatcag aaatatcagt ctcggggaat
     2761 acccaaaccg atatcgtcat gtcctggttg tcgacaattt gcatcctcag gcctgagcct
     2821 tcagtcgcag cgtcaaggat gggaggcaga gagtcgttcc gaccgtccca gttgttgata
     2881 gcgattttcg ctttcagaat gatgcggtac gtctcatcgc ttagcgtcgt atagccagaa
     2941 tcaggatcat atggcccttt ccagatgccc tggtcatacc caagcccgtc agtgtcccag
     3001 ctgaaataaa ctccgctaat tggctggctg actatgcgac tgcgtccgat ccacagaccg
     3061 aggatgtcga gctgtacacc gacagcagta tcaatatcga aggctgttat aagccctgac
     3121 atagtgctgg acacatcaat cagcgggcgg gtgctcagat ctatatggtc aaaaaagagt
     3181 ggcttggtag cgtggtagtt agtgatcagt tcggtgtatt tgctcatgag gtcaccgtga
     3241 tactgatatt cgcggtgcta caggacgcag aagcatcata ggcaatatca atgtttgatg
     3301 ccgatacgct gccagacgac ttaccgatca gcaggtcggt aatatcgtaa tagcgggcat
     3361 tcccgccgct cacaacgccg aggtttgccg gggaataaat acggctcagc agaacgtcgt
     3421 cgccaattgt taggccattt atataatcgg caacagcctg tttaatctgc tcgccgattt
     3481 gagaggtata gccggtaaaa actttcaggg taatggctac gaaaattggc acatcggtag
     3541 agcgcgaaaa actgatgacg tgtggattac cgtaagtatc cggcaccgtg acagaagttt
     3601 taccgtaagt tgcggttccc tgccctttac tcccccggat tgtttgggct atctcggtaa
     3661 catcccctcc atcgacgatg gcggaaatag agtgtggcgg cagcccgttg ctgtcggttg
     3721 ccccagtgtc gttctcatat agtttgtgac gtgtcacgcc agcaacatta gcgatagcac
     3781 cgtcgacacc ttcaaacggt gtgatcgatg gtagcgcgac gctttgcccc tgccgaatgc
     3841 gcagctctgc gtcggtttcg gctggtgaac cgacagtagc cgcaactgga ttggttaccg
     3901 acacccaacc tcgggtcggg gtgttgatag tggtaatagt cccggccagc gccgcaaccg
     3961 aaccgctatt cgcacatgtg gccgtcacca gcacagtacc atcaacgccg atcgctacac
     4021 ttgcgggaaa attccagata atgccgtttt tatcccgtgc ggagccattc gtgatagtcg
     4081 tgcctgccgt accggttaac agaaggtcag cagtagagtt tgtcgctact tttcgcgtga
     4141 tcccgttaat tttcacattg ctgctaagcg ctgcggcctg cgctgtcgtc ggtgaaaaag
     4201 agttgtagat cccgatagcg gtgttgttag cgtcatgcac ggcaagagcc accagcgcga
     4261 ccatctgccc gtctttgctg tctggttcga ggtaggcatc actaccgtaa atctgcctga
     4321 aatagctggt cagtgtatct aggattgtct ggtaatcagg cgcactaatc ccctgggcgg
     4381 ttaccgttgc cgatagcccc agcgtgtcga ggttcaaagc catttatgcc tcgcttgtta
     4441 cagtcgtctg gccgtagatt gtgtcaatgg aggaagtgaa ggtgacgcga cggctggtgc
     4501 cgtcataatt ggtatcgaag gaaagaatcg acagaacgcc cggtgtatcc tgtatgcgtt
     4561 cgcgtatagc caggatgtag acatctgatc gctgcttccc aagcactgac tgaacatacg
     4621 gcgtgccttc cgtcagatcg agaaaccact gaccgcgcca cagctcgaaa cgggttttta
     4681 cggcctgggc gacacattcc ggactgtcga taaggaaagt atcgtcaccc tggccgaaag
     4741 tgtaatcgcc ttcagtatct tcgcgacggt atcgcattat tgcggccctc cggtagttcc
     4801 cccgccagtc tgtactccgc catgtttatg cgtggcgaca ctgataccag aagctgttac
     4861 gtcattcgtt accatcaccg gcccaagcat cgtcgcagta ccgccgcttt cgcccattcc
     4921 ctgagacaag ttgccattaa tcgttacgtt gccgttcagc gtgatagtcg gggatgtgat
     4981 tgtcgttcca ccttcagccg tagctgtaag ctggcccggt gttttaatgg tgatgttatg
     5041 tcctgcggcg acttctacaa acgccgcacc atcatcggtt cgcagctgcg cggcgctggt
     5101 actgataccg ctgattttcc gtgcttgcga ctgcgggcca acgatggcga acgcatcaga
     5161 taagtcatgc tggcgcgggt cgacggtctc ctgaacgccg ccgctctgcc accaaaaatc
     5221 gatgcaacgg tcagcaaaaa tcaggaggca ctcgtcgcct tcttttaccg gaaaggtcag
     5281 cgtgcaaccg ccgccgcgcg ggaagatgac cggcacatcc accagcggtt ttaattcggt
     5341 ggatccatcg ccaacaatac cgcgtagcgc cacctctact gtgcaggtaa cagtctcagg
     5401 atcgaacgac tgaatgatgc cgggcatcgc tacgcgcatc tgggtagaca ccgaatcggc
     5461 aatggcctgc gcggtctgct gctcaccgcc gatctgtgat tgagttggaa ttggcataaa
     5521 acccccataa aaaaaccagc cgaagctggt ttggtatcaa ttatctctta gtaactaagc
     5581 tacatcagtg tgaagtaagg cttttccgct gcaatggcac acattcacaa ggtgtattag
     5641 gatatgtttg caacaagtaa gcattgaaaa tcaatttcag ccatgaagcc gccatagagt
     5701 ggacctgcac catcgcatta ttgactacag gagtaacaaa tgggatttag atttcgtaaa
     5761 agaatccgga ttgcgcccgg actcgcgatc aacattagta aaagcggagt aagcacttca
     5821 attggtggta aaggttccac cattaacatc gggaaaaaag gcgtaaagat gacaaatggc
     5881 cttcccggca cggggctgtc gcataccact aacctttact ctcccgggaa atcgacagaa
     5941 aaaaaacaac ttactcataa gcaaaaaata attagaaaca tcctgttcgt aattattgtg
     6001 tttataataa ttaaagctca atatttttga cgcatgcccg cctatctggc gggctatttt
     6061 acttttcggc agtcgtatgt tgcatactga cgcggcgaat ccatgctggc ttgcagccac
     6121 tgtgcgttga gaatggcttt gccgtttcga ttgatgtact caagaccaac ccatcttcca
     6181 ggctgatcgg tagccatacg ccaatccatc ttgatattgt tataatcgcc tttgtttttc
     6241 aggaaggtga ttttttgcat ttctggcttt gcgccattga ttctggctaa gccatcatct
     6301 gcccaatgga ttttaaaatc accacattgc tgatccgcaa aagcgggtgc tgatataagc
     6361 accgcgaaca cggtaacagt gcaaagtaaa tgtctcacgg ccccacctca ctaattcgtc
     6421 gtcccttgca ttgctttcgg gctataaaga tcacgagcgc cacgcgcaaa acacatcaaa
     6481 tccatgtacc acgcctgacc tctggtgtcg ccagtatagt cgatagcttt gacgatataa
     6541 acgccatccg tcgcaatgct ggcagcctgt gacgtcgtgc cggtcagcac acggttgccg
     6601 ttctcttctg tttcggtgat acgcccgggc gactgtgcta tttcgctatt gccgagcgtg
     6661 gcgcggtaca ccgaagcctg atcgagctgg ataagaccat taatacggat gtttgggttt
     6721 atcaggcatc gcacgtttac tccgccaccc atcgtttgtt gcggcatacc gatcaggcca
     6781 gtattggcat tcaacacaat ggcttcgtga atatatttat cctccggcac catctggacc
     6841 tgaccatcca ccagttgcca tgtcgctttg cactgcgcag caatattatc catcacgttg
     6901 cggctggatg agtaaatcgc gcggccacga ggaaacacgg tatcaggaaa atcgccggta
     6961 atgccctgtg tcacgccgaa cgcgttgaaa tcctgcatcg tcgcccggtg cagatccgca
     7021 acggtatatc cagcggcaag cgtggtgatg gtggtcgcat agaggaacgc ttcgtgatca
     7081 ccaatggcct gaatcaacac ccaggaatcc gtaatgttgt ccttcccggt gacggtgaag
     7141 cgaatatcac cgtcaaaaat caggccgtag ttctgaccgt tcacctgccc tatctggtct
     7201 ggtgaaatct cccgggacac accaacctgg ctcgcatcaa catccggcgc tataccgtca
     7261 tacccggcaa tgatgcgaat tttggcaaac tcctgcccaa gtatcttgtt cgtggtatcg
     7321 gtcgaaaggt tgtaaatttt cacgttcgcc acgcgcggcc agcgtgtgtc tgcccactcg
     7381 atctggaacg tgaccttaaa gtcagacagg gaaacgccct gcccgttctg gtccaacagt
     7441 tgcagctcaa aatggcgcat ccagttaaga gacatttcta ctcctgtacg aaaatgaggt
     7501 ggctgtatgt gccgaggttg gttttggtgg gctcgtctgg tgcgccctta tcggtcgcca
     7561 ccaccagcgc gccatcaatg ccaagctgtg gatattgtcg taaaaggttt acgccggtca
     7621 gtagaggtac gccagagaga agcgcggcac cgccgctatc catcacgtcc ataatccagc
     7681 cagccgcatc acgccagatg atcctgagtg tatacgtggt attgcccagc aaaactcgga
     7741 actgctgatt gtcaggagaa agcggtattt cgttaaactg catatcatcc cccgaatgct
     7801 gatgtaacgc ttccgcccag ctggctcagc aaggattcgt ttggcggtgt agtggatttc
     7861 atcccggaat tctgcaccgc cgatgtgctg acgccatcct gcatatctga tttatctgca
     7921 acgctaacgc tctgcgtctg cgacatgatc acttcacgca gggtaagcgt gcagttcagc
     7981 acgttctcgc tggttttatc cgttgtcacc tcgatggctc gcaccagcat attgctgtaa
     8041 acccgctttc cggtcactac atcgaacggc accctagagg actggagatc cagtagttgc
     8101 tgataggtct ctttcgggct aagtccggcg ctgaggccga ttgacgatgt atcaatgaag
     8161 tccagcaacg aaccgccacc tgcgaagccg cattccatcg tgacttcgct ggggcgctta
     8221 tacgcatgat cggcgatgaa acccgacgcg ctattcgttg ttggcttctc caccgggtgc
     8281 tcagtaattt cgagcgcatc agaatgcttt tcagaaacaa ccacgctggg gattaatatt
     8341 ccaattcgcc gggattgctg gcgaaaaatt gctgataaaa tatccattat ctcggtcctg
     8401 cggggagttg ctgggttaac tgtgaattca cgcccttttg acggtcaaca gtcaaacggg
     8461 cagcctcgcg cggatcgaaa acgccgtgga tgttaatatt cgtttcctgc tgaatcaccg
     8521 gggcgctggt gggcatattg ctcattactt tcggaatgta gttgcgcgtt tcctgcggca
     8581 ttagccccat tccataacgc ttaacattcc cgatccccca gttatatgat gccagtgctt
     8641 tgctaaggtc tccgccgttc tgccgcaaca gctggctcag gtacttagcg gctgcctgag
     8701 ctgacttttc cgggtcgaat acatcattcc cacgcaggcc catgtcgcgc gccgtgccat
     8761 ccataaactg aaacagacct ttagcgccag cgcctgaaac ggcgaactga ttaccgcccg
     8821 attccgttat ggccacgctg cgcaataacc cctccggaag ccggtagagc tgttccaggt
     8881 tggtaaacat cggctgcatc catcccagca gttcagagcc tgctttggtt ggctgtggcc
     8941 gcttaactga ctggccgagc tgctcaggct catcatcacc gaaccagccg cgcaccgttc
     9001 ggcccacgct gcgaggatcg aatccccagt gctctttaat ccagtcggcg gttccgttgg
     9061 cgctgtctgt taccatcggc atcgctgacg gattttcgct gccctgatta agcatctgtt
     9121 tgccgatgct ggcggcatca gcccagcggc catctttaat ggcgttgagc aggtcggcga
     9181 tcatgttcag catcttgccg aactcaccca tttgcgaaat gaagttgcta aaatcccact
     9241 ttagggacca tgacttgggg tcaataccga gcaacttagc cagcgctttt gtcaggtccg
     9301 taacagtctt tttcaggtca ccaatcattt tgacggcctg atcaatttca gtcttccatt
     9361 tcccccagtc aatcaggctt ttgccacctt ctttccacgt ctggtaatca tccaagagca
     9421 gaccaagtgc aataataagc gtcgttatca tgcccaaggg tgatgacatg aaagcggtat
     9481 ttagcagccg ccatgcaacc aacagcccac tgaacaatgc gataagctgc tgcgtggctg
     9541 ggttcagctt tttgaaccag gcgatcacac cctcaacagc ttgcccagtt cgccataaca
     9601 cgcgggtaat cgcgtcacct gcccagagaa ttcctttgat gatcttcgtg atgaccgctt
     9661 caatcttcgg ccagttgtcg agtatctgcc tgcggaagtt atcaatactg ccagcaaggc
     9721 caccagctag gttagagcca atcttgtctt tcgcctgtcc aagcgtcatc gtcagattac
     9781 gcatggaggt catgaaaata ttggactgtt tagccgctga ctcagcatta aaaccaatac
     9841 gctttgccgt caacgcgtac tcagaactga gctgccccat ccctctgcgc atcgccatca
     9901 gcgtgttttc atcgatgccg agcatctgcg cgtattgctt cgcgcggtaa tacggcatgt
     9961 tgttgagctt ttggccaacg ccagtaaaga tggcagcagt atcacgcatc tttccgctgg
    10021 catcgcgggt ctggacacca agacggttca ggaacccttc cgcccccgga ttgctacgca
    10081 tgaagccagc caacccttcg agggaggaca tagccgactc ggcgctggca ccggtttgcg
    10141 atgcggcgta tcccagcgct ttgatgccct ggacactggc ccccgtccgc tgggatgccc
    10201 agtaaatttt atccagaccg ttcgcgatct gagtggtaaa tccgacaatg ctcagcgctg
    10261 cgcctttcac caccgcgccg accttcagaa cgttcgcggt aacgcctttc agcacggctt
    10321 caaacttatt agcgccagcc tgatcgatat cgaatcccag cgaaacaagg aaatctttaa
    10381 tcgtatctgc gttaccgctc attggccgct ctccatttat ctacccgggc gtcgttatcc
    10441 tcgcgcatgt cgaggtagtc attgagaagc gcgatgcggc aaagatctac cgcaccgctg
    10501 ttaaggtctt tctggtcaat atggaaggca agcgccgggc gaagaataaa atcttcaccg
    10561 cccggcaggc tgttgaaggt tattccgcta gcggggtggg cgtctcgctg gtagggagtc
    10621 cttgcaaaaa atttcccagc gagtcggcga ccacccgcgc caccagttgc agcatggtaa
    10681 gcaggtcgat atcgtcaaac gccatttcgc catgctggca gaccggcacc cagcctttca
    10741 tgtgctcgcg tgaaacaacg gaaagacagg ggaacaggat agcgtccacg tcgccatcac
    10801 tcagatcgga cacagcattg gcaatctttg gcaggatagt agccatcgcg ccttcggtgt
    10861 ctttgctgct gatcttctcc tgaacgctcc ggaagtccga aaccatcccg gccagaaccg
    10921 gcaacagctt gcgggacacc ttcagctgtt cgaaaacgct gagctttgcg gtgcgatatt
    10981 tcacgccttt aatttcgaat tccatgcgtt aaaactcccc gagaagctgg tcaatcttgc
    11041 cgcagtcgaa tacccaggcg acggttccgc cctctttagc gttattgaaa tcaggctgtt
    11101 tctggaatgc acacgaacgc gcagtagaaa tatcacccga tgccgtgttg cgaatgacga
    11161 tcacgttatt gccccaggtg gcagaggact ggctttgcgc gttatacgcc agagacagct
    11221 ttttattcac cggggaggtt ttgagtagcg tcaccgtaat ggtgcctgac ttatcggcgt
    11281 gcaggctgtg catcacctcg ccatcggcac cgatagtcat ggtgtttttg ttgccgccca
    11341 tggtctgggt gataccttcc tcagagttcg cagaaccctg accaagatcg ataacgccgg
    11401 tcggcccggt gagcgacgcg gttacatcga gaaaagaata agttgccatt tatcgctcct
    11461 tagcgaacca cgttgatctg cacatcggca taatgaactg cgccagccag cttacaggcc
    11521 acctggatta acggtgcttt gcgcgcttca cggtcggcct gcgcctgttc ggacagcggt
    11581 tgcgcataca cgtaatagcc tttggtcagc gtatcgccgg aattcagctg tccgataggg
    11641 ccaccattcc acacgccagc agctaccaga ccgttcgtga cggactgatc catggactgt
    11701 tcaacgttgg aaagcagacg ggtcacaccg gcatcagtct gcggaatttt ggtggtgctg
    11761 gtgtaaagca ggttatagag gttggtctga acgtagttct gcaaccagtc gagcccgtgg
    11821 cgctcgtcga agaagtcacc gttcgccatg acaccctgtt gcaggatcgc cgtgtcgttg
    11881 gcgtagtaca cgaatacatt gcagtttttt gcatcaagtg ccgatgcctg gctgactgtc
    11941 agtgtttcat acccgacacc cggctcctgc ttaaacttga gcgtaatcgc ggtattactg
    12001 ccattgaaat taaccgtgaa tgcccggcca aatgcagata acgcagcgta tttattaccc
    12061 gatgaatatt gaataaaact gcgtgaatat ccggcggttt tcagttttga tgccaaatca
    12121 tcgctggatg cagtctgcag gcatttctca tcgcttgtcg taatcgccag aatacggctt
    12181 acagaagagg attcgatcgc cgcagccact ttcagccagt ctgcatccgg aatatcttca
    12241 tcgtctgcaa tccccagccc ataccatgaa gtataatcga gcatggcatt cacagcctgc
    12301 tccagcgtct caggcgtggc ctgttcgctg tctcccttcg ttttcaccca acgaccaaca
    12361 aaaacctcct gaggtttcgg tgattgtgag aaaaacacct gcgcagcctt atattctggt
    12421 gattccacgc caaaatcttt tccaatatct tccgcggcag aataacggcg aatgcgctca
    12481 cttaccggaa tgattgtgga cgggccgaga atgagtaatg caccaaaatt tcgccctgat
    12541 gctgcacgcg gcgacatgat cacatcaaca ttaacaacgt ttgatacagg caagccctgt
    12601 gccatagctt aatctccgaa aaagatgact ggtgcttcca ccagcgattt aataccgtac
    12661 tcgcgcacaa ccttccggcg cagacgcacc gtcatatcgt agcggcggac ccattgctga
    12721 ttaataagtt cagggaagag acctgtgtaa tcgccaagag acagccccag cgcattcagt
    12781 gctgcgttgt tctgcggtac agatataccg tcacgaaacc gggacgcata caccatcccc
    12841 gccggaccat aaaacgaagc catacactca atcgtttcat gccgccagag ctgagagcca
    12901 tcatcggtct gtctggtgaa tgccggactg tcatcacctg accatccgat aaccccaaac
    12961 gcacaccagt tcgtttcaac cggtagcagt ggcggctgct ctttctgcca gcgcgggcga
    13021 accatcccgg cagacagacc ggaaacgtta cgcatccact ggcttaacag cctgtcgagc
    13081 gcttcgtcat aatccggatc gccactggtt ggtattaacc atccgcgctc tgtactggtg
    13141 ttattgctca accggagttc ccccatcaaa cggcatcaac tcacaatgcg cctgaacgaa
    13201 tccggcccca taagctgtat acgggtcgac gaaggtcaca cgataatcac ggccctgata
    13261 cgtcacgata tcggcatcac ggccagtctg tccctgcgtc agtcgctcag tcgtcacaat
    13321 cagaattgca ccgctgatta cctgccctgc ctgcatacgg cggttttcca gagagcgatc
    13381 aacagttacg actccggcaa actgcttttt aacttcactg tcgctgccga tcccgtcctc
    13441 atccaccgtt tgcactcggc gtgttaccca caaattgaag tcgcaaaaat cggggtcaaa
    13501 aagcacatct gttacatcaa gagtcggcat ctttatccct cacaacatgg gtaatagctc
    13561 tgcgatattg cccggtgtca attaatggtt tcgccagatc ggttccggga gattcgccag
    13621 caacacgccg agcaagttcc agtgttgccc ccttgcgccc ccgacgagcc cgggcttcaa
    13681 cggtgctatc agcaagcggt gtaaagccgg taatggtcat gtaacgcctg acgccattcg
    13741 cggccagcgt tccggcgcgg ttaagcgctc tttccgcacc cgccgcattt ccatcaagcg
    13801 cagcctgcgc cgctgctttg agctgcggca ccgtctgttc ctctacggat ttaacgccgg
    13861 ggatcaggtg cgggcgtggg ggtatgtttt gcgctggtga gccgtattcg ttgacgtaac
    13921 cgatcccggc attaccaaac ggaacatcct cacgctcgct gtcttcttcc gggatgccca
    13981 ccagcacatc ctttttggtt agcgaccgga gcgcatccag aatggcctga gcgttatcaa
    14041 ccctcgttgt tacaccactt ttgaaactca tagctggcga ccgcccgcac cgaacatcgt
    14101 gatcagctga taaaattcag cgccatatcg ggtgttattc cagaagcctg cgtcagggtt
    14161 tagcgtcgcg ctggtgtcat agctgacgct taccttgtca acggacttgg aggactgaac
    14221 accattggtt gaaccgcccg ggccgccaac cagcattgcc cgactatctg ccgcccatag
    14281 cgtcatgtag tgagccacga acaacccggc aaagtacgga aacaaatctt tgccggtgac
    14341 gttttcgctc agcagttcat cggccagatt cagacgaaac tggatttgcg cttcgggata
    14401 tttggcaggg tcagcaaact gcgggaagtc gcggcgaaaa tcacttaccg ctggcaggct
    14461 ttgattcttt ggcatttttt acctcgttac gcgcgtctgt ggctttgcca acggatactt
    14521 ccgcgtgcgc acgagtgaac cagtgcgtgg caacgtcttc ctccactgca tgacggcctt
    14581 taacaaactc gcgccgcgaa ccgtcgggaa gcgtgagcac aaacggggta tgtacgtgta
    14641 ttactgcatt attttttgcc atcgggtcat ccttaatggc cccgccaggg gggccatgtg
    14701 gctgttaaat gccatcaacg tacgaaatgg tttctttgta cactggctca accgcaccca
    14761 gcttgccgta gtaggtcgca atctgataca gaccgcgata ctggatagga acgctctgca
    14821 acggcaccag cggatagcgg acgtattttt tatcgttggt gtacgcaacc atgcgatcct
    14881 tattccccac accacggcct ttcagccatt taaccgcgcg gatattcagc ggaacaccgt
    14941 tctggtgata gctgatggtg ttggtctgaa ggtacgtcaa cagggactgg ttacccgcag
    15001 atgaaacgat gatgctggac aacagagcaa actgctctgg cgggatcagc aaatcacgcg
    15061 gaaccacaga gtaaccggaa gcggcccacg catcagacag cacctggtta atgcttgcgc
    15121 ggatttcgtc cggtgttgag attgcccacg ttttggcagc gttgttgaca ggcacgccgt
    15181 ccagggtaac aaggcctttc aggtttaatg cggaatcgcc aacatacacc tgctcgtcgt
    15241 tgtccatctg ccatttgagc tgcatcccgt cgtacttctg ggtgtcaatc gggcgaccta
    15301 cctgctgagc tgctgccagc tctacaacgg tccagcccag ttccatgccc cagagattca
    15361 gcgggttacc ggatttgccg atatccacgt ttacgccagc aatagcggtt gagtctttgc
    15421 ctacccagtt tttgccattc ggatttgcac cagtacccgc agcggcgaag ctggtattcg
    15481 tccagctgga aatgtcatct gcgatagaga catcttcacg caactgaata tcgcgggtcc
    15541 aggtgtaccc cgccagtggc aggttcagag tctggtcgag tcgctccagc tctccgatga
    15601 gaaaggcacc agagctgtca acggttgcct gatcaaaagt aatcattcgt ctgttcctta
    15661 aatcttccag gaaatttctg cattgccgtt agcatcaccg gcacctgtga attcagcgtt
    15721 ggtcagcacc acatttttgc cactgactga cgtggacatg aatccaccca gcggcacttt
    15781 gatggattca tcagtggaga cgacaacgta taccgggtcg ccttttttga tggtgctggc
    15841 atcaaaatca gaaccgagat taacggtcac gtagccacgc ttcatggcgt cgcccgggaa
    15901 gttcttgcct gttcccacct ggcgaaccat gtccggctgc gaagtggtcg gataagggcg
    15961 cacgtagatc cccttcacct tgtctgcggt atcgccatct gccagcggca cgaaaaaacc
    16021 gtcagcatcg tatttaccag ccagcccata ggcagcaaag gcgttatcgg atttaaggac
    16081 caccggttcg acggttaagt cctgcgggcg agagatagcc ccggcaatgc caacaggcat
    16141 ccggtacaga tatgcagtca ttggattatc ctttgcggtt agaccagaat tcagcgtttt
    16201 gtttgttcag ggaagcgatg ctggtcatgc ccatgtttgg acgttgtgca tcgcccgtgg
    16261 tgctgcgggt gtttcgccct ttggcaatct cagacacagc gttaaacgcc atatcgaccg
    16321 attgcttagg caatttgcgg atatccgcat caccgacaac ctggcgaacc agcgttttgt
    16381 cagcagaagc cagaacctcg cgtttgaaca cggtcggttt catcttacgg ctcagatcga
    16441 tacccggaac gataacttcg gcacgccagg ctgagtcacc agtaatcgtg gtttcctctt
    16501 catcgtcctc gccgtcaccg gtcggattat cgtcaggttt attatcgtta tcgcccgtgg
    16561 catttccttc cagcttagcc agcagggctt tcagtaatgt tttgaggtca tcatcactgt
    16621 cgccggttgg acctccgccc atctctggta ctttgtccgg tagcggttgc tgcggggaca
    16681 ggttgatgtt gagattaacg ccctgcggca aatccccctc atctcctgta accgatgcgg
    16741 gagccgactc caccagttcg ttcatggtgt cagcgtcacc cgttttgatg gccgtgcgca
    16801 tgcgggtcca ccagcttttc ttttgatttg ccattgtgtc tctgtctcca attgcacaac
    16861 gatttccggc tctgcctttg gggacaagag ccacatggtt tccggtaata tcgacctgct
    16921 cggctttacc tggctcggtc tgctcgtact ccgcgtcata gccgcacgac acttcacgca
    16981 ggccatcttc gataagctga atggcgtttt cgtctttgac gataaggtca gccagcatca
    17041 aatcagagtg gtcacccgtc ccgcgccgca cattctgaag atgcccgaca gcaagctctt
    17101 tccagttctc gggatttacc agccgcacat tcccgttttc atcttcagga tgcaggatcg
    17161 tgatgctcat cccttcgaat gaggcgagcg tggccggatg gaatacctgc tcaggagagc
    17221 gcgttacgac tatctcaccg agcttgtcgg gtttgaggtt tggcagatcg gcagcgccgt
    17281 agagctgttt acccgttcga cctatcggca cgtctttgca cagcagcgag ccgtcagcca
    17341 gctgatagcg ggtttccccc agccgggtat tgaaaaaata tttcatggtt tacctgcgat
    17401 tcaggcgaga taagaatgag ggttgggaaa aacgatttct ttataacagc gacaattcgg
    17461 gagctcgcca gcgtgaccgg tcatgccatc aagcgttgga ggtttgcccc attcgacaaa
    17521 cttaccttcc atctcccgat gagaatgccg gacgtcgcca tcttcggctg tacgccagat
    17581 ataaccattc gagccgattg acagcgcacg cgcctgatcg agcgcgccgg ttgcacgtcc
    17641 aagctcggta cgggcgataa ggttcgctcg tgagcgtgac acgtcaccgg acgctgctat
    17701 ctctttcgcg aatggttcag cgcggccacc agccacaacg gcctcgatgg ccttgttctg
    17761 aatgtcatac acccgatcgg cggcctcaag aggcagtgac ttgatatact taatttgctc
    17821 ggcgacgatg gatttcatca cctggcctac cggggcgcgg tcgaccatgt tgcgcagctc
    17881 tgcgctgatg ttccggctgt gctgacgcca ctgcttttca ttctggcgtg caatgtcggc
    17941 ggtaaagttc tcagcaacct tcgtcgccca gggggtgatg atttcgctgt agcgctccag
    18001 cgcatccatt atttcggtaa cgctatcatt tgaaccatcg tagcgcccat ttacgatatc
    18061 cccgaccgcc cgcgctatct gccgtaggct cgttcgatat cggatctccg cctggcggct
    18121 ctggcggttt gtcgccaagt tcgccgatgc ctggcggcgc ttcgtcttcg gcattctcga
    18181 tatcctcgtc ggtaatggat gccccgatgc cggtgacgtc agagttttcg cgcaggtcgg
    18241 tcatcgccgc cttacgcgtc atcaatccgt cgcccagcgc ggtactaatc gcgttggtgg
    18301 tgtttacggc caccgttgat cggtcaacgt ctgacatttg ccatagcggg ttaaactcaa
    18361 acgtgaaatc gtccggcagc ggctttccga gttccgagcg gtgcataatg tccagtatcc
    18421 ggcgcatcgg cagccgtaag cggcgctcct gcaatgagct cacccggtcg taatagttgg
    18481 cgaggtctgc atcaccagta gagaagcctt tcggggattg accgaacaac cgcaccagcg
    18541 ggataccaac ggcaccgcta atctgctcag caaactgcga aagaatgtca tccagaccac
    18601 taaagctgta ctggtgggtt tcgaacttat cccgcgagtc catgagcgtc ataccttcat
    18661 tgctctggaa ctggcggatc aggtcgatgt tcttcagcaa cgcctcgaac gccgggcctc
    18721 caagcgcgat aagctcgcgc aacttctcca cgctataggt acgcagatgt gctttgtaga
    18781 ccagctgcgc cgcgccgaca gtggcgctgt cgaacgcagt aagccgatcc cagatacgct
    18841 ctacaaccga cattccccat tcgttttcgg tcatcttctg ctggaatggc agcgtcaccc
    18901 cgtcgaagcg aatcaggcgg ctgtgatgga tgcgccaggc cgggatgccc gttgcagtgg
    18961 tcaccacgtc gtaaaactca ggtttgccga gatctggccc catctcttta atgcggcggg
    19021 tcaggaccgg gttaatcatc cagcggtcga gcgggagaat gcccttaaac ttgccttctc
    19081 caatggtttc gagccgcagc ggggtcattg gtgcctgccc ctcgatcatg atgaagccga
    19141 ccgcgccgcc gtagaggcga gaccatttca gcacgtcgtt cagcgcatcc cagatttgca
    19201 actcatccag ttgtgattcg agaatgccac gatcttttgc atcaatttcc gaagtgatgc
    19261 gaatgccttt gcgggtcata tcatccggga tagcatcgac cgcttcgccg ataatccagg
    19321 acgaacgata ggaccattcc accagcatgc ggttacgact ggtgaaatta gcccggtagg
    19381 tggatgctga gtgctggtta ggtgtctgca tccccacgcg ggcaacaaag ttctcatagc
    19441 catcagcggt ggcctgtgcc gttcgccgcg tggcttgttt gtttcgtgcc atcaggcctg
    19501 tctccctagc agctcccaga tgttcagggc tgaattcatt ggcgcgtagc tgatcatcac
    19561 cgagtcggca aggtttggcg accgggttcc atcaggctgt ttatcaataa cgatttttcc
    19621 cacaccatta atggaatagg tcggctgcga aagctcgatg atgagtttat ctttgagtgc
    19681 catgctactg ctgattgaga tgatttcgtc cgggttgtaa tccatacctt caaccacggc
    19741 gcgccaggta ttctgaaaaa gtttacgtaa ccgccaccag ctctgggctt tggcgttagc
    19801 gaagaagtcc ttgttcaggc gggcggcctg tccgttgtca ccgcgcaccg cttcgtcgtc
    19861 cggatcaaat accgcgccac tacctcgaaa cggtgtggcg agtattgacg gtcggcgcgc
    19921 agcgttacgc agttcgttga tagcgcgtgc atcgccgcga acgccagcgc ccaacccgtc
    19981 ctcgtcaaag cgaaactctt cgaggttgtc ctgttcgcaa aagccgaaaa ccttctcgac
    20041 ggactgataa atgtcgctgc ccacaccgga ccattcccgc acattctcca ggaggaagcc
    20101 atgacgggtg gaaaaggaat ttttgtccct gccttcgtcg gcgacgtcca tcgcgcccag
    20161 tcgcttgcct gttggctgga tgcccagctt gatatgcgcg tcgacagcag cctgtaccca
    20221 ttcggatgga atcaggacgc cttccgctga tgcactgtag ttcaggtcaa gttcctgtgc
    20281 caccaccacc ggattgtcga ttttctcgca ttccctgcga taccactctt catccttgcg
    20341 aggatcatcc cgccagtgga atgtgaatac cggtatcttc ccgccatgac gcttctgagc
    20401 gaacgggttc gccatgccgt taactgaact caggtcaata cggcaacgcg tcgtttgtga
    20461 caacgccgca tcaatcagca gaggacgctg aaggaatgca gactcatcaa ccagataaag
    20521 cgtggtacgg tcaccacggc caatattatc gccagcctca cctttgataa cggcaccagt
    20581 ttcaggaaac tcaacgcgca tgtatggtgc gtgcttcttt tcgtcccacg aacctcgaaa
    20641 ctctacaggt agtgtttcca cgaacttgcg cgccttccag aacaatgctt tcgggtcacc
    20701 ggtgctgtcg acgtattcct ctttacggga gccgaaaccg ataaccattt ctttgttgaa
    20761 gagacaaagc gagcatgcca gcccgatcgc ggtccaactg agccccattt cacgggattt
    20821 ttcggtaata ccattctccc gattacccca gcgttccata atccagtgga tccactcctc
    20881 ctgcttaggg aagagtaaaa acggaatggt caccggcagg ccataatcaa tattacgcgg
    20941 gtccgttgtc atgccccagt cgatgatgaa ctgagccgga ttggttcggt aaaactgttt
    21001 tagtgcaggc aatatttcag gattctggcg aatgcgctgt aggcgttcca tccgccattc
    21061 aaaaaccatc tggtaatcag gatgtttaaa atcgaagggg aatggtaacg gcatacttag
    21121 cccatcattt ttctatacgc ctctgcagcc tgctccggcg ttaagttggt aatttctgtt
    21181 ctgactggtc ctccgtcagc accagtcact tcatttttga cattgtcttt aaacgcctga
    21241 acagaaacat gacgcccgag caactcaagg tttttaacct tatcaggcca tttgattttc
    21301 ttcaggagag cagcactatc tgctgatgcc atctcgacga catccattcc tgatagcgtt
    21361 gtacgccaca ccttaggcca gtctttaatc tgcttcagtt cgccattggc aagcagaatg
    21421 tcaagcacgt ccatctgatc gatttcagtc agccgtcgca atacataggc ggcgtctata
    21481 cctacctctt cattgcgttc agcctttagc tcagcaatgc gatctgctat gtcaggtttt
    21541 gacaggttct cgctaccagt ggcgcgagca gttttctcgc tgtaacccgc cctgatagct
    21601 gcctgagtgg cgtttaaatc ttttaaatat tccctgcaga acagttcctg cttaggtgtg
    21661 agctgtttca aatcacacct ccgaaacatt gctccaattt c
//
LOCUS       DAEEDS010000063        21506 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEEDS010000063 DAEEDS010000000
VERSION     DAEEDS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.063"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(38..2107)
                     /gene="glyS"
                     /locus_tag="KLH32_002702"
     CDS             complement(38..2107)
                     /gene="glyS"
                     /locus_tag="KLH32_002702"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBE0139469.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="KLH32_002703"
     CDS             complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="KLH32_002703"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBE0139470.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVGF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3123..3422)
                     /locus_tag="KLH32_002704"
     CDS             complement(3123..3422)
                     /locus_tag="KLH32_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBE0139471.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3597..4592
                     /gene="wecH"
                     /locus_tag="KLH32_002705"
     CDS             3597..4592
                     /gene="wecH"
                     /locus_tag="KLH32_002705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBE0139472.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4634..5071)
                     /locus_tag="KLH32_002706"
     CDS             complement(4634..5071)
                     /locus_tag="KLH32_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBE0139473.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5117..5458)
                     /locus_tag="KLH32_002707"
     CDS             complement(5117..5458)
                     /locus_tag="KLH32_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139474.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="KLH32_002708"
     CDS             complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="KLH32_002708"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBE0139475.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="KLH32_002709"
                     /pseudo
     CDS             complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="KLH32_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8841..9833
                     /gene="xylF"
                     /locus_tag="KLH32_002710"
     CDS             8841..9833
                     /gene="xylF"
                     /locus_tag="KLH32_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0139476.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9911..11452
                     /gene="xylG"
                     /locus_tag="KLH32_002711"
     CDS             9911..11452
                     /gene="xylG"
                     /locus_tag="KLH32_002711"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBE0139477.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11430..12611
                     /gene="xylH"
                     /locus_tag="KLH32_002712"
     CDS             11430..12611
                     /gene="xylH"
                     /locus_tag="KLH32_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBE0139478.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12689..13866
                     /gene="xylR"
                     /locus_tag="KLH32_002713"
                     /pseudo
     CDS             12689..13866
                     /gene="xylR"
                     /locus_tag="KLH32_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13974..14798)
                     /locus_tag="KLH32_002714"
     CDS             complement(13974..14798)
                     /locus_tag="KLH32_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBE0139479.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15118..17148
                     /gene="malS"
                     /locus_tag="KLH32_002715"
     CDS             15118..17148
                     /gene="malS"
                     /locus_tag="KLH32_002715"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBE0139480.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17326..18579
                     /gene="avtA"
                     /locus_tag="KLH32_002716"
     CDS             17326..18579
                     /gene="avtA"
                     /locus_tag="KLH32_002716"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBE0139481.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18730..19203)
                     /locus_tag="KLH32_002717"
     CDS             complement(18730..19203)
                     /locus_tag="KLH32_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBE0139482.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="KLH32_002718"
     CDS             complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="KLH32_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBE0139483.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20354..21352
                     /gene="yiaK"
                     /locus_tag="KLH32_002719"
     CDS             20354..21352
                     /gene="yiaK"
                     /locus_tag="KLH32_002719"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBE0139484.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21364..>21506
                     /locus_tag="KLH32_002720"
     CDS             21364..>21506
                     /locus_tag="KLH32_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBE0139485.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5400 a         5489 c         5283 g         5334 t
ORIGIN      
        1 agcctgccag atggcaggct ttttaataac ggcgttatta ctgcaacagc gaaatatccg
       61 caacgcgcag gaacagttcg cgcagtttct ccagcatggt cagacggttg agacgcaatt
      121 ctttgtcatc aaccatgacc atcactttat cgaagaaagc atcaaccggc tcacgcagct
      181 ctgccagttc gaccagcgca tcctggtaac gaccttccgc aaagtacggc tccagcttgt
      241 cacgtagcac cacaacctgc atcgccagtt taatttcttc cggctctttc agggtagagg
      301 cattcacgcg atcgctcagc acttcgtcag atttcgccag gatgttagat acacgtttgt
      361 tcgccgccgc cagtgctgca gctgcttcca gggtacggaa gtgcgatacc gctttcatgc
      421 gggcatcgaa atcagccgga cgagtcggac gacgcgccag taccgcctgg atggtgtcaa
      481 cggtataacc ttcgtcctga taccaggcgc ggaagcgacc gagcataaag tcgataacat
      541 catcaactac gttggcatta gtcagcttat cgccatacag acgcaccgct tcttcggtca
      601 gcgtttgcag atcaaggttg aggttcttct caacgataat tcgcagcacg ccaagtgcgg
      661 cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg ctgaccgata ccgaagatac
      721 ccgccagagt atccatctta tcagcaatcg ccagcgcaca agctaccggg ttggacggca
      781 ggtcatcacc ggcaaagcgc ggctgatact gctcattcag cgcgacggct acgtcttccg
      841 cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc ttgggtgtcg gtgaactcga
      901 agaccatgtt ggtcatcagg tcacacttag acagcaggcc cgcacgggtt gcgtggttaa
      961 cgtcagcgcc aatctgttcg gcgatccagc cagccagcgc ctggatgcgg tcagtcttgt
     1021 cacgcagcgt acccagctgt tgctggaaca gcacggtttg caggcgcggc aggttatctt
     1081 ccagacgttt tttgcggtcg gtgttgaaga agaactcggc atccgccaga cgtggacgaa
     1141 cgactttctc gttaccagag ataatttgct gcggatcttt cgattcgatg ttggcaacga
     1201 agataaagtt cggcagcagt ttgccgtcgt tcgcatacac cgggaagtac ttctggtcac
     1261 ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag gaatttctct tcgaattttg
     1321 cggtcagaac gaccggccac tccaccagcg aagccacttc ttccagcagg ctttcgctta
     1381 agtcagcgtt accgccaatc ttacgcgctg cttcttcggc atcggcttta atcttcgctt
     1441 tacgttcttc gtaatcggcg atgactttcc cgcgctcacg cagaatttcc ggatactgat
     1501 cggcgttgtc gatggtgaat tccggttcgc ccataaagcg gtggccgcga atcacgcgat
     1561 cggactgaat gcccagaatg gttgccggaa tgactttgtc gcccagcagc agggtcacgg
     1621 tgtgcaccgg acgcacgaag tgcacgtcgc ttgcacccca gcgcatcagt ttcgggatcg
     1681 gcagtttcgc cagcgaagtc gcaaccatat tcggcagcag tgcttcggtg ctttcgccct
     1741 tcacatgggc gcgatacagc agccattcgc ctttatcggt agtcagacgc tcagcctggt
     1801 caacggtaat accacaacca cgcgcccaac cttctgccgc tttgctcggt tttccttcag
     1861 cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat ttcacgatct ggttgtgctt
     1921 ccgccaggtt agctactttc agcgccagac gacgcggagc agcaaaccat tgaacggtgc
     1981 cgtgtgcgag gcctgcgtta tccagctccg cagtaaagtt cgcagcaaag gactctgcca
     2041 ggctgcgcag tgcttttggt ggcagctctt cagtgccgat ttccaccaga aaagttttct
     2101 cagacatagc cgcctcttac ttatctttgt tgcacatcgg gaagccgagg gcttcacggg
     2161 aagcgtaata tgcttctgcc actgctttgg tcagggtgcg aatgcgcaga atgtagcgct
     2221 gacgctcggt gacggagatg gctttacgcg catccagcag gttgaagctg tgggcggctt
     2281 tcagaatacg ctcgtaggct ggcagcggca gcggattttc cagcgccagc agctgctgcg
     2341 cttctttctc gtactgctca aagcaggtga acaggaagcc cacatccgcg tattcgaagt
     2401 tgtaagtgga ctgctccact tcgttctgat ggaacacgtc gccgtaggtg gttttaccca
     2461 gcgggccgtc gctccagact aggtcgtaaa cgctgtctac gccctgaatg tacatggcca
     2521 gacgttccag accgtaggtg atctcgccgg taaccggttt acactccaga ccaccaacct
     2581 gctggaagta agtgaactgc gtcacttcca tgccgtttag ccacacttcc cagcccagtc
     2641 cccaggcccc cagagtcggg ttttcccagt tatcttccac gaaacggatg tcgtgaatag
     2701 tcgggtccat gcccagctct ttcagagaac cgaggtacag ctcctgaata ttgtccggcg
     2761 atggcttaat gaccacctgg aactgatagt agtgctgtaa acggttgggg ttttcgccgt
     2821 agcgaccatc ggtcgggcga cgagaaggct gaacataagc agccgccatc ggttctggcc
     2881 ccagcgcgcg cagacaggtc attgggtgag aggttcccgc gccgacttcc atgtccaatg
     2941 gttgaacaat ggtgcagccc tggcgagccc agtaatcctg taaggtcagg atcaagccct
     3001 ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc tggatacgtg tggatttaaa
     3061 ggaagggatc agtatacccg ctggatggaa gatatacagt acgaaacggg aaaaagcagg
     3121 gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt caaaagaaca gacaaactgc
     3181 tctttacgga aggtataacc gcgcatttca tagcttccct ggaactgttc gaaggcggtg
     3241 acgtcgattt tttgctcgcc ggtgttataa cgttttgccg cctgatcctt gcataacgct
     3301 tccatattca acgaacgcag aggatcaacc tttacccgtt gtgctttctg cacgggtgac
     3361 ggtgtagaac aacccactag caccataagc gccattgccg gaaagaaagc gttcatcatc
     3421 attaaattac cgtctggttt gcaggtgagt cttattattt atatcggtat agtggctgat
     3481 atcttacgaa tttccttaag cctctatagg ctgtagtgat aaaactcaga ttattccagt
     3541 gcgccaacca aatgatttat cggtcagact ttttttaacc agatacagag acacagatgc
     3601 agcccaaaat ttactggatt gataacctgc gagggatagc gtgtttaatg gtggtgatga
     3661 ttcacaccac tacctggtat gtgaccaatg ctcatagtgt tagccccgtc acatgggata
     3721 tcgccaatgt tctgaattct gcctctcgtg tcagcgtgcc gctatttttc atgatttccg
     3781 gctatctctt ttttggcgaa cgcagcgccc agccgcgcca tttcttgcgt atcggcttat
     3841 gtctgatttt ttatagcgca atcgcactgc tctacattgc gctgtttacc tccatcaata
     3901 tggagttagc gctgaaaaac ctgctgcaaa agccagtgtt ttaccacttg tggtttttct
     3961 tcgcgattgc ggtgatttat ctggtttcac cgctgattca ggtgaagaac gtcggcggaa
     4021 aaatgttgct ggtgctaatg gtggtgattg gcattatcgc taacccaaac acagtgccgc
     4081 agaaaattga cggttttgaa tggctgccaa ttaacttata tatcaatggc gatacttttt
     4141 actacattct gtatggcatg ttgggccgcg ctatagggat gatggacaca cagcataaag
     4201 cactgtcgtg ggtgagcgcc gcgctgtttg cgacgggggt ttttattatc tctcgcggga
     4261 cattatatga attgcagtgg cgcggaaatt ttgccgatac ctggtatctt tactgtgggc
     4321 cgatggtttt tatctgcgca atcgcgctat tgactctggt taaaaacacg ctggatacgc
     4381 gtaccattcg cggacttggc ttaatctccc gccattcatt gggtatatac ggattccacg
     4441 ccttgattat ccatgcgctg cgcacccggg gaattgagct taaaaattgg ccaatactgg
     4501 atattatttg gatcttttgc gcgacgttgg cagcgagttt gttactttct atgctggtac
     4561 aacgaatcga cagaaacaga ttagtgagtt aagtaaaagc ccgttcacat tggactgacc
     4621 gggcttacgt gaattattcg ctgtattctt cctgggtaac ctgtgtttct tttggcgggt
     4681 ttattccggc atcacgaata ttcttctgca ccgctactgc accaaacaag cttaagaaga
     4741 aagccagtcc ataaaaacct ttttcactga gtaataatgt cgcattccac agacctacca
     4801 tcagtagagc aacagagata ataaagacag tcaggcaggt catataataa atggaagtgg
     4861 tcggtatgcc ttcatacttg tcccgaacgg tcttttgata agacgcagca gaaaacagtc
     4921 ctaacaccag tacggcaaaa taataccctt tttcatttaa ctgcatctct gcattccata
     4981 gccctaacag gtaggtaacg ataccaccaa cgagagctat ccatgacaca atactaaagg
     5041 ccggtgaata ggttgatatt ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt
     5101 aagagcttta tgttgctcat accgagcgaa acaaacgcat ttgaccaaac aagcagacaa
     5161 aaaaggcgat ggggtagatc accatttcat aagcattgat aggtgcattt aaaacaccga
     5221 taaacaagag accgatagtg attaacgcaa ctaactggca gatatgggta aaaaaattat
     5281 ccagttgccc gagtttttct gcgcgaagat atgcatagtt accaaaagtt gctgtcatta
     5341 acacgccaag aaaatagcct tttccactta gcaatgggca tgagatccat agcccaacca
     5401 gataaaccag cgccccgaca acaaataata gttttgccac agtttttgag gttttcataa
     5461 cgatctccat atctaccagc gatacattac gagtaaccaa cgaaagacaa aactgaaaaa
     5521 tgccattaac aaatgatttt cagaataaat tcatactaaa tattaattaa ttagtgagat
     5581 atatagatgt gaattatccc ccacccggtc aggcagggga taacgtttac gccattaatg
     5641 gcagaagttg ctgatagagg cgacggaacg tttcacgtcg tggctgataa gcggcataac
     5701 gctgcgcatc tggtagatgc gactgttcta acggtagctg cggcaacaac tcaatgagcg
     5761 atttctctgg attcgccgca atctgcgcca gccttgctgc gcccagtgct ggccccacat
     5821 ccccccccgt acggtaatcg agctgctgac cgctgatatc cgccagcatc tgacgccagt
     5881 actcactacg cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat
     5941 gcacgacatc catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca
     6001 gttcattggg gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat
     6061 tgtgtggtgt acgctcgccg gaaagataag gcagaaacca aactggctcg gcactttcat
     6121 cagcctgttg agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg
     6181 cccaatccag acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta
     6241 gcgcatggca aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag
     6301 caaaatagac ccccgacgtc cccagcgata acattgcctg attagcatca accattccca
     6361 caccaactgc accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac
     6421 cccacgcttt cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg
     6481 cgggcatctg gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct
     6541 ttgcgacatc cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg
     6601 tcatacgcag acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct
     6661 ccggctcatg ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt
     6721 tgccggtaat cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac
     6781 agcgcccgtc gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg
     6841 ttgctccatg catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat
     6901 cgcccagagc tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg
     6961 accaaagtgg atgcgggcgc gaaacggtca gcttttccgt ttgcgaagca accacctcac
     7021 cctgctcgtt gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca
     7081 tatcgatcgt tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg
     7141 cacagttagc cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg
     7201 gcgaccactc tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc
     7261 cagtgacatt tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg
     7321 ctgcgcgatg cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc
     7381 cagtgccatc gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact
     7441 ttgacgacgt actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag
     7501 aatttcatac atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca
     7561 gcccagttgc gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc
     7621 ggtggctatt tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt
     7681 cagtttaatc tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc
     7741 gtaatcatat tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgcccta
     7801 gaagccgatt ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg
     7861 ctcctgacgc aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac
     7921 atagttttca ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc
     7981 ccagctgaag acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt
     8041 aaagcagtta gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag
     8101 gacatcaacc atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga
     8161 aacatccaca tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc
     8221 gacatctgct ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt
     8281 aaacgccccc acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc
     8341 ggcaaaacgc aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg
     8401 acggaatgct aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc
     8461 aaaataggct tgcatattga actccataat caggtaatgc cgcgggtgat ggatgatgtc
     8521 gtaatattgg gcactccctt tcaattgctc aattatgtta tttcacactg ctattgagat
     8581 aattcacaag tgtgcgctcg ctcgcaaaat aaaatgggat gatgaaactg ggtaattcct
     8641 cgaagataaa aatgcaataa gtacaattgc gcaacaaaag taagatctcg gtcataaatc
     8701 aagaaataaa ccaaaaatcg taatcgaaag ataaaaatct gtaattgttt tcccctgttt
     8761 agttgctaaa aattggttac gtttatcgcg gtgattgtta cttattaaag ctgtcctcta
     8821 actacagaag gccctacacc atgaaaataa agaacattct actcaccctt tgcacctcac
     8881 ttctgcttac caacgttgcg gcacacgcca aagaagtcaa aataggtatg gcgattgatg
     8941 atctccgtct tgaacgctgg caaaaagatc gagatatttt tgtgaaaaag gcagaatctc
     9001 tcggtgcgaa agtatttgta cagtctgcaa atggcaatga agaaacacaa atgtcgcaga
     9061 ttgaaaacat gattaaccgg ggtgtcgatg ttcttgtcat tattccgtat aacggtcagg
     9121 tattaagtaa cgttgtaaaa aaagccaaac aagaaggtat taaagtatta gcttacgacc
     9181 gtatgattaa cgatgcggat atcgattttt atatttcttt cgataacgaa aaagtcggtg
     9241 aactgcaggc aaaagccttg gtcgatattg ttccgcaagg taattacttc ctgatgggcg
     9301 gctcgccggt agataacaac gccaagctgt tccgcgccgg acaaatgaaa gtgttaaaac
     9361 cttacgttga ttctggaaaa attaaagtcg ttggtgacca atgggttgat ggctggttac
     9421 cggaaaacgc attgaaaatt atggaaaacg cgctaaccgc caataataac aaaattgatg
     9481 ctgtagttgc ctcaaacgat gccaccgcag gtggggcaat tcaggcatta agcgcgcaag
     9541 gtttatcagg gaaagtagca atttccggcc aggatgcgga tctcgcaggt attaaacgta
     9601 ttgctgccgg tacgcaaact atgacggtgt ataaacctat tacattgctg gcaaatactg
     9661 ccgcagaaat tgccgttgaa ttgggcaatg gtcaggagcc aaaagcagat acctcactga
     9721 ataatggcct gaaagatgtc ccctcccgcc tcctgacacc gatcgatgtg aataaaaaca
     9781 acatcaaaga tacggtaatt aaagacggat tccacaaaga gagcgagctg taagcgttac
     9841 gccccggcgc ggagcggggg cgtgatttct ctccatgccg cgtgaatgaa ttggcttagg
     9901 tggagtcgtt atgtcttatc tacttgaaat gaagaacatt accaaaacct tcggcagtgt
     9961 gaaggcgatt gataacgtca gcttgcggtt gaatgctggc gaaatcgtct cactctgtgg
    10021 ggaaaatggg tctggtaaat caacgctgat gaaagtgctg tgtggtattt atccccatgg
    10081 ctcctacgaa ggcgaaatta tttttgcggg agaagagatt caggcgagtc acatccgcga
    10141 taccgaacgc aaaggtatcg ccatcattca ccaggaattg gccctggtga aagaattgac
    10201 cgtgctggaa aatatcttcc tgggtaacga aataacccac aatggcatta tggattatga
    10261 cctgatgacg ctacgctatc agaagctgct cgcacaggtc agtttatcca tttcacctga
    10321 tacccgcgtt ggcgatttag ggcttgggca acaacaactg gttgaaattg ccaaggcact
    10381 taataaacag gtgcgcttgt taattctcga tgaaccgaca gcctcattaa ctgagcagga
    10441 aacgtcggtt ttactggata ttattcgcga tctacaacag cacggtatcg cctgtattta
    10501 tatttcgcac aaactcaacg aagtcaaagc gatttccgat acgatttgcg ttattcgcga
    10561 cggacagcac attggtacgc gtgatgctgc cggaatgagt gaagacgata ttatcaccat
    10621 gatggtcggg cgagagttaa ccgcgattta ccctaatgaa ccacatacca ccggagatga
    10681 aatattacgt attgaacatc tgacggcatg gcatccggtc aatcgtcata ttaaacgagt
    10741 taatgatgtc tcgttttccc tgaaacgtgg cgaaatactg ggtattgccg gactcgttgg
    10801 tgccggacgt accgagacca ttcagtgcct gtttggcgtg tggcccggac aatgggaagg
    10861 aaaaatttat attgatggca aacaggtaga tattcgtaac tgtcagcaag ccatcgccca
    10921 ggggattgcg atggtacccg aagacagaaa gcgcgacggc atcgttccgg taatggcggt
    10981 tggtaaaaat attaccctcg ccgcactcaa taaatttacc ggtggcatta gccagcttga
    11041 tgacgcggca gagcaaaaat gtattctgga atcaattcag caactcaaag ttaaaacgtc
    11101 gtcccccgac cttgctattg gacgtttgag cggcggcaat cagcaaaaag cgatcctcgc
    11161 ccgctgtctg ttacttaacc cgcgcatcct cattcttgat gaacctacca ggggtattga
    11221 tattggcgcg aaatatgaga tctacaaatt aattaaccaa ctcgtccagc agggtattgc
    11281 cgttattgtc atctcttccg aattacctga agtgctcggc cttagcgatc gtgtactggt
    11341 gatgcatgaa gggaaactaa aagccaacct gataaatcat aacctgactc aggagcaggt
    11401 gatggaagcc gcattgagga gcgaacatca tgtcgaaaag caatccgtct gaagtgaaat
    11461 tggccgtacc gacatccggt ggcttttctg ggctgaaatc actgaatttg caggtcttcg
    11521 tgatgattgc agctatcatc gcaatcatgc tgttctttac ctggaccacc gatggtgcct
    11581 acttaagcgc ccgtaacgtc tccaacctgt tacgccagac cgcgattacc ggcatcctcg
    11641 cggtaggaat ggtgttcgtc ataatttctg ctgaaatcga cctttccgtc ggctcaatga
    11701 tggggctatt aggtggcgtc gcggcgattt gtgacgtctg gttaggctgg cctttgccac
    11761 ttaccatcat tgtgacgttg gttctgggac tgcttctcgg tgcctggaac ggatggtggg
    11821 tcgcgtaccg caaagtccct tcatttattg tcaccctcgc gggcatgttg gcatttcgcg
    11881 gcatactcat tggcatcacc aacggcacga ctgtatcccc caccagcgcc gcgatgtcac
    11941 aaattgggca aagctatctc cccgccagta ccggcttcat cattggcgcg cttggcttaa
    12001 tggcttttgt tggttggcaa tggcgcggaa gaatgcgccg tcaggctttg ggtttacagt
    12061 ctccggcctc taccgcagta gtcggtcgcc aagctttaac cgctatcatc gtattaggcg
    12121 caatctggct gttgaatgat taccgtggcg ttcccactcc tgttctgctg ctgacgttgc
    12181 tgttactcgg cggaatgttt atggcaacgc ggacggcatt tggacgacgc atttatgcca
    12241 tcggcggcaa tctggaagca gcacgtctct ccgggattaa cgttgaacgc accaaacttg
    12301 ccgtgttcgc gattaacgga ttaatggtag ccatcgccgg attaatcctt agttctcgac
    12361 ttggcgctgg ttcaccttct gcgggaaata tcgccgaact ggacgcaatt gcagcatgcg
    12421 tgattggcgg caccagcctg gctggcggtg tgggaagcgt tgccggagca gtaatggggg
    12481 catttatcat ggcttcactg gataacggca tgagtatgat ggatgtaccg accttctggc
    12541 agtatatcgt taaaggtgcg attctgttgc tggcagtatg gatggactcc gcaaccaaac
    12601 gccgttcttg attttgataa aaattttctc aaagccggtt acgtattacc ggttttgagt
    12661 ttttgcatga ttcagcagga aaagaaccat gtttactaaa cgtcaccgca tcacattact
    12721 gttcaatgcc aataaagcct atgaccggca ggtagtagaa ggcgtagggg aatatttaca
    12781 ggcatcacaa tcagaatggg atattttcat tgaagaagat ttccgcgccc gcattgataa
    12841 aatcaaggac tggttaggag atgtcgtcat tgccgacttc gacgacaaac agatcgagca
    12901 agcgctggct gatgtcgacg tccccattgt tggggttggc ggctcgtatc accttgcaga
    12961 aagttaccca cccgttcatt acattgccac cgataactat gcgctggttg aaagcgcatt
    13021 tttgcattta aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc
    13081 ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag
    13141 tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa
    13201 aatcggctgg cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact
    13261 gacgcccgag cgcggcatat tctgcaagta tgtgaacata tacatattcc cgtaccggaa
    13321 aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc
    13381 ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa actgttgcat
    13441 cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc
    13501 attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat tcaggccatg
    13561 cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc
    13621 gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat
    13681 gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg
    13741 tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt
    13801 tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg
    13861 ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg
    13921 cgagcaaaag ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata
    13981 tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc
    14041 ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga
    14101 tccgctttac gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc
    14161 aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc
    14221 actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta
    14281 ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc
    14341 atactggtag ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta
    14401 ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca
    14461 ggtgaccatt ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct
    14521 gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt
    14581 ccggaagggt attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac
    14641 tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct
    14701 gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga
    14761 atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg tcggatttaa
    14821 tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa
    14881 atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg
    14941 attcagattg gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt
    15001 tgcgcgcgat cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt
    15061 tgtctggctt atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg
    15121 aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact
    15181 tctccggggt tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag
    15241 ttgcccaaag gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca
    15301 gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa
    15361 tggcgattgt tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca
    15421 acattgatga tttccatcca gaacgccgcc gaaccggtag caaacctggt ccgtgaatgc
    15481 ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc
    15541 gccgtacggg attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta
    15601 caacccgctg ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc
    15661 gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac
    15721 ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc
    15781 acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg
    15841 ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggc
    15901 ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg
    15961 aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat
    16021 cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg
    16081 gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa
    16141 acgctgggtg aacgctggag cgactggaaa cctgccgccg ggcaaacctg gcatagcttt
    16201 aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg
    16261 atcagaacgg atatcggcga ttacgacaat cctggattcg acgatctcac tatgtcgctg
    16321 gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat
    16381 aaaaacaaaa cggatactca cgctaaagtc atcgaaggct ttacgcctcg cgattactta
    16441 acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc
    16501 gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt
    16561 cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc
    16621 ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg
    16681 atgatcaatt tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg
    16741 gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc
    16801 tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta
    16861 ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct
    16921 acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt
    16981 aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc
    17041 gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt
    17101 gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt
    17161 cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg
    17221 ttgcgggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc
    17281 gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt
    17341 tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac
    17401 ggtttacgca cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa
    17461 atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat
    17521 gcactgtgta actacgacgg tccacagggg aaaacggagc tactcacact gcttgccgga
    17581 atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc
    17641 agccagagcg cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg
    17701 gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg
    17761 gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt
    17821 aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc
    17881 tcccggccga cgaatccaac aggcaatgtg attactgacg aagagttgct gaagcttgac
    17941 gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc
    18001 ccgggtatca tcttcagtga agggcgcccg ctatggaatc cgaatatcgt actgtgcatg
    18061 agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa
    18121 atcatcactg ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt
    18181 ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc
    18241 atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta
    18301 ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt
    18361 aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg
    18421 atggtgccgg ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa
    18481 tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg
    18541 gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga tgcgctacgc
    18601 ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata
    18661 aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcgttg agaaacgccg
    18721 gaaaacgttt caaacaggct gctgttgtag ccgatgccgc tgcactttct cgacatccat
    18781 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca
    18841 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
    18901 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat
    18961 atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc
    19021 acactgatga caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga
    19081 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
    19141 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
    19201 catgatcatt cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
    19261 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgctc
    19321 aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt
    19381 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
    19441 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
    19501 agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg
    19561 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
    19621 ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
    19681 gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt
    19741 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg
    19801 gtttcaccag tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc
    19861 agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct
    19921 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
    19981 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
    20041 ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc
    20101 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
    20161 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc
    20221 acttgatttt tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga
    20281 tatatttgaa atctagttcc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaac
    20341 aaggaagcct aggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt
    20401 aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac
    20461 caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact
    20521 ggaaaacggc gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat
    20581 tgaacagtgg gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga tgatggatcg
    20641 cgccattgaa ctggctgccg atcacggtat tggtctggtg gcacttcgta atgccaacca
    20701 ctggatgcgc ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg
    20761 ctggaccaac tccatcgccg taatgccgcc gtggggcgca aaagagtgtc gcatcggcac
    20821 taacccgctg atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtc
    20881 gatgttctct tacggcatgt tagaagttaa ccgcctggca ggccgtcagc tcccggtcga
    20941 tggtggcttt gatgatgagg gcaatttgac caaagaacct ggcgttatcg agaagaatcg
    21001 ccgcattttg ccgatgggct actggaaagg ttctggcatg tcgattgtgc tggatatgat
    21061 cgctactctc ctttccgacg gcgcatcggt tgccgaagtc acccaggaca acagcgacga
    21121 atacggcatt tcacaaattt ttattgccat tgaagtggac aagcttatcg acggtcccac
    21181 ccgcgatgcc aagctgcaac gtatcatgga ttacgttact accgctgaac gcgctgacga
    21241 aaaccaggcc atccgcttac ccggccacga atttactacc ctgctggccg aaaaccgccg
    21301 taacggcatc actgttgatg acagcgtgtg ggccaaaatc caggcgttat gaggagataa
    21361 gtcatgattt ttggacatat cgcgcagccc aatccgtgcc gtttgcccgc cgccattgaa
    21421 aaggcgctcg attttctgcg cgctaccaac ttcaacgtcc tggagccggg cgttgtcgaa
    21481 atcgacggca agaatattta tgcgca
//
LOCUS       DAEEDS010000064        21387 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEEDS010000064 DAEEDS010000000
VERSION     DAEEDS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.064"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..134)
                     /locus_tag="KLH32_002721"
     CDS             complement(<1..134)
                     /locus_tag="KLH32_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139486.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="KLH32_002722"
     CDS             complement(236..1216)
                     /locus_tag="KLH32_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBE0139487.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="KLH32_002723"
     CDS             complement(1224..1934)
                     /locus_tag="KLH32_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBE0139488.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KLH32_002724"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KLH32_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBE0139489.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="KLH32_002725"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="KLH32_002725"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBE0139490.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="KLH32_002726"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="KLH32_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBE0139491.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="KLH32_002727"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="KLH32_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBE0139492.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="KLH32_002728"
     CDS             8511..8621
                     /locus_tag="KLH32_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBE0139493.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KLH32_002729"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KLH32_002729"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBE0139494.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KLH32_002730"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KLH32_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBE0139495.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="KLH32_002731"
     CDS             11503..12135
                     /locus_tag="KLH32_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBE0139496.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KLH32_002732"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KLH32_002732"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBE0139497.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="KLH32_002733"
     CDS             15667..16599
                     /locus_tag="KLH32_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE0139498.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="KLH32_002734"
     CDS             complement(16640..18070)
                     /locus_tag="KLH32_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBE0139499.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="KLH32_002735"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="KLH32_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBE0139500.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="KLH32_002736"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="KLH32_002736"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBE0139501.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="KLH32_002737"
     CDS             complement(19931..20200)
                     /locus_tag="KLH32_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBE0139502.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="KLH32_002738"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="KLH32_002738"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBE0139503.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="KLH32_002739"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="KLH32_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBE0139504.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5233 a         5365 c         5143 g         5646 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgc
//
LOCUS       DAEEDS010000065        21277 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEEDS010000065 DAEEDS010000000
VERSION     DAEEDS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.065"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..446)
                     /locus_tag="KLH32_002740"
     CDS             complement(<1..446)
                     /locus_tag="KLH32_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBE0139505.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KLH32_002741"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KLH32_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBE0139506.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KLH32_002742"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KLH32_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBE0139507.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KLH32_002743"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KLH32_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBE0139508.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="KLH32_002744"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="KLH32_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBE0139509.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="KLH32_002745"
     CDS             complement(5216..6814)
                     /locus_tag="KLH32_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0139510.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KLH32_002746"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KLH32_002746"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE0139511.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="KLH32_002747"
     CDS             complement(8493..9071)
                     /locus_tag="KLH32_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBE0139512.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KLH32_002748"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KLH32_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBE0139513.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="KLH32_002749"
     CDS             10510..10689
                     /locus_tag="KLH32_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBE0139514.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="KLH32_002750"
     CDS             complement(10809..11168)
                     /locus_tag="KLH32_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBE0139515.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="KLH32_002751"
     CDS             complement(11530..11874)
                     /locus_tag="KLH32_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE0139516.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="KLH32_002752"
     CDS             12006..13916
                     /locus_tag="KLH32_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBE0139517.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="KLH32_002753"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="KLH32_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBE0139518.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="KLH32_002754"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="KLH32_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBE0139519.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15495..17180
                     /gene="fadD"
                     /locus_tag="KLH32_002755"
     CDS             15495..17180
                     /gene="fadD"
                     /locus_tag="KLH32_002755"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBE0139520.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17262..18377
                     /gene="rnd"
                     /locus_tag="KLH32_002756"
     CDS             17262..18377
                     /gene="rnd"
                     /locus_tag="KLH32_002756"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBE0139521.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KLH32_002757"
     CDS             complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KLH32_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBE0139522.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KLH32_002758"
                     /pseudo
     CDS             complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KLH32_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20411..20977)
                     /locus_tag="KLH32_002759"
     CDS             complement(20411..20977)
                     /locus_tag="KLH32_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0139523.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21189..>21277
                     /locus_tag="KLH32_002760"
     CDS             21189..>21277
                     /locus_tag="KLH32_002760"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0139524.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5269 a         5272 c         5467 g         5269 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggca ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt attgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggt tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgggtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcggtcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga cgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt cccacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaaaa agaaacagcg gctggtccgc tgtttctgca ttcttacggt aaagataaaa
    15361 ataaatagtg acgcgcttcg caaccttttc gttgggtaat tatcaagctg gtatgatgag
    15421 ttaatattat gttaacagtg tgtatatcat ttggggttgc gatgacgacg aacacgcatt
    15481 ttagaggtga agaattgaag aaggtttggc ttaaccgtta tcccgcggac gttccgacgg
    15541 agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct
    15601 acgccgatca gcctgcgttt gtgaatatgg gggaggtgat gaccttccgc aagctggaag
    15661 aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag aaaggcgatc
    15721 gcgttgcgtt gatgatgcct aacttattgc aatatccggt ggcgctattt ggcattttgc
    15781 gtgccgggat aatcgtcgta aacgttaacc cgttgtatac cccgcgtgag cttgagcatc
    15841 agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgcc cacgctctgg
    15901 aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg ggcgatcagc
    15961 tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag cgcttggtgc
    16021 cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac ggctaccgga
    16081 tgcagtacgt caaacccgaa ctggtgccgg aagatttagc cttcctgcaa tacaccggcg
    16141 gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg
    16201 aacaggtcaa cgccacctat gggccgttgc tgcatccagg taaagaactg gtagtaacgg
    16261 cactgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt atcgaactgg
    16321 gtgggcagaa cctgcttatc actaacccgc gtgatattcc agggttggta aaagagttag
    16381 cgaaatatcc gtttaccgct atcactggcg ttaacacctt gttcaatgcg ttgctgaaca
    16441 ataaagagtt ccagcagctg gatttctcca gtctgcatct ttccgcaggc ggtgggatgc
    16501 ctgttcagca agtggtggca gaacgctggg tgaaactgac cggacagtat ctgctggaag
    16561 gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa cccgtatgat attgattatc
    16621 atagcggtag catcggtttg ccggtgccat cgacggaagc caaactggtg gatgacgacg
    16681 acaacgaagt accaccgggt caaccgggtg agctttgtgt caaaggaccg caggtgatgc
    16741 tgggttactg gcagcgtccg gatgctacag atgagatcat caaaaatggc tggttacaca
    16801 ccggcgacat cgcggtgatg gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag
    16861 acatgatttt ggtttccggt tttaacgtct atcccaacga gattgaagat gtcgtcatgc
    16921 agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag
    16981 cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac cgaagagtca ctggtgactt
    17041 tttgccgccg tcagctcacg ggatacaaag taccgaagct ggtggagttt cgtgatgagt
    17101 taccgaaatc taacgtcgga aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca
    17161 aagtggacaa taaagcctga gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt
    17221 tttttgacgc ccactaaaga gaaaacaatt tgaattacca aatgattacc acggacgatg
    17281 cgctggcttc tttgtgtgaa gccgtccgtg tctttccggc gatagccctg gatactgaat
    17341 ttgttcgtac gcgcacttat tacccgcagc tggggttgat tcaacttttc gatggcgagc
    17401 atctggcgct aattgatcct ctcgggatca ccgactggtc accgctgaaa gcgatcctgc
    17461 gcgatccgtc catcacaaaa tttctccatg caggcagtga agatctggaa gtgttcctca
    17521 atgtctttgg cgaattacca caacccttga ttgacacgca aatccttgct gccttctgcg
    17581 gacgcccgat gtcatggggt ttcgcttcca tggtggaaga gtattccggc gttacgctgg
    17641 acaagagtga atcgcgcacc gactggctgg ccagaccgct gaccgaacgt cagtgtgaat
    17701 acgcagcggc ggatgtctgg tatctgttac cgatcaccgc caagcttatg gtagaaacgg
    17761 aggcctccgg ctggctacct gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc
    17821 aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac caatgcctgg cagttacgca
    17881 cacgccaact ggcctgtctg caactgttag ccgactggcg actgcgcaag gcgcgagagc
    17941 gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt gtggtcggtc gcgcgttata
    18001 tgccgggaag tttaggcgaa ctggacagcc tgggtttatc cggtagcgaa atccgctttc
    18061 acggtaaaac gctgctggcg ctggtggaaa aagcgcagac attgccggaa gatgccttac
    18121 cgcagccgat gcttaacctg atggacatgc ctggttatcg taaagcgttt aaagcgatta
    18181 agtcgctgat tactgacgtg agcgaaacgc ataagatcag cgccgaattg ctggcatcgc
    18241 gtcggcaaat caaccaactg ctgaactggc actggaaact gaaaccgcag aacaatttgc
    18301 cggagctgat ttccggctgg cgtggtgagc tgatggcgga agcattacac aatttattgc
    18361 aggaatatcc gcagtaaaat cttccgaagc cggactgggc gcgctcagcg ccacatccgg
    18421 ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt cgcacgcgaa caacagatca
    18481 acatactttg ctggctggca cgctcttcat cgctaaaata ctgatcccga tggtcagctt
    18541 caccttccag tattgccgtt tcgcaggttc cgcatacccc ttcacgacat aaacattcca
    18601 ctttcgcggc tttattattt tcaataacct gcaaaatagt catctcttcc ggcaccacaa
    18661 actcttttcc ggaacgggca agcaccaggg taaatgcatc gccggttttg tcttcgatag
    18721 caaattgctc aaagtgcagt gtatcggcgg cgatgtccag acgcgcagct tcacttctta
    18781 ccgcttcaat tagcgcctcg gggccacagg tataaacgtg tgtgccaggt tcgatatccg
    18841 ccaataatcg cgctaattcc agtcgtgttc cggttgatga atgatgcaaa tggactttct
    18901 cagcctgcgg atgctggact agctcagcac gaaatgcgca actttctgga tttcgcgagc
    18961 agtaatgtag ctgccagtcg atgtcgctgt gttgcaactc tgccatgtgc gacaggaaag
    19021 gggggatacc aataccgccc gcgataaaca gatgctttct ggctgaggga attagcgcaa
    19081 acaggttatt aggcgttgag atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa
    19141 agcgggaacc gccgcgcgag ttttcttcca gtcgaacggc aatctgataa ctagaggtgt
    19201 tatgcggtga actcagtagt gaatacgcat tgctgtactg gttatcacca tcgctcatct
    19261 gcactaggat gtgacttcct ccggtgaacg caggcagcgg tttgccatcg gttgccacca
    19321 gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg gctcacctgt acttcaaaca
    19381 tttgatagtc tgacattgct gtactccatc aaaaatcagc ccagcggcgg aaaataccgc
    19441 cgccgatgtc attagtcctt aaacacctgc gccagcagat tatggaaatg ggcgatacca
    19501 tgttcggaaa tgccactacc gctactgtcg gccatgatgc gcccctgacc acgatagcca
    19561 cgcgatttca gccctttctg tacgctttca accagacgta aatcttccgg atggaacaca
    19621 tcgcgatacc actcaatcag cgatttttgc tcgtcggtta actcttcatt ggtgaagtaa
    19681 atatcgtagt tttgcagggt agtttcagaa tccaccggga attcataaat gaccgtcatc
    19741 atccctttga tcggggtgac gttcagcatc gtgcacggcc acagccagaa accgtggaat
    19801 gccgcatccg taccctcttc aaatttaaac gactgttcgg acggtttggc gaaaccgtat
    19861 tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat caacctgtac ggagtcggag
    19921 aaacctagat gcgccggacc acagtgatag cactcgagat agttatcgac aatgttcttc
    19981 cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt tcagattgtg gacttccggg
    20041 caggcttcca gcactttcgc gcccagaccg gtgtattgat tcacttgaag tacgaaaaaa
    20101 aaccgggagg acattggatt attcgggttc tgatgggatt agatttggtg gggcttgcaa
    20161 gcctgtagcg caaattttag tcgttaatca atgaaacgcg aaagatagta aaaaattgct
    20221 tttgtttcat tgaaaatacg aaaaacaaaa acactgcaaa tcatttcaat aacagcttca
    20281 aaaaacgttc aaaaccgata acaaccaggc tgtaaccaaa tgactcatat cacaaatcag
    20341 ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg
    20401 ttcatggttg ttatacggct ttttttacat cttgtggttc ttgtgaagga gctacaagac
    20461 cttcctgttc ataaatacca tactttgaca aaatcttttt acgagtatct gttggtaatg
    20521 attcaagcat tttggctgtt tgtaaggttt gctgcgaaat accttgcaaa tcagcctgga
    20581 tggaatcatc ggaagaaaaa atctcaggca taagagcttc aaatcctttc tttctgaaca
    20641 actcaatcgc agaatgcagt tccttgtcct ctaacaattc gacaattgcg atcagtttag
    20701 ttttcaatgt gctgcggtca ggttgagaat ttttattctc ttgattattt ttatgctcaa
    20761 ccattggcat gctctgactt tggataaacc actctattgg acgccctgtt gcgtaggcta
    20821 tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga caggtagttt ctgataaccc
    20881 catcagaaag tccgcactta gctgcaaacg cccggcttga ttctccattc attgcctctt
    20941 caaggcgttc cttgaaacgc attatttccg ggggcataaa aatgcgctct gcatttttgc
    21001 tttctttttc acctgtcatg cccaatctct ctgttatata aagaatttta cagaattctt
    21061 tgatttgcac tggttaaaat aaaaatgagc atagctcttg aattgagcat ttttctgcgg
    21121 tatcgttttt cccgatagcg gtagccggta tcagttaccg aaaacgttaa gagagtaagg
    21181 atcgcagaat gtcagcaaaa aaccaagact ggcacgcaga gcagataaag gctgctgttc
    21241 gtatgaaggg gaagacacta tctcaattat ccagaga
//
LOCUS       DAEEDS010000066        21255 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEEDS010000066 DAEEDS010000000
VERSION     DAEEDS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.066"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..793
                     /locus_tag="KLH32_002761"
     CDS             <1..793
                     /locus_tag="KLH32_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBE0139525.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="KLH32_002762"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="KLH32_002762"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBE0139526.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KLH32_002763"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KLH32_002763"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBE0139527.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KLH32_002764"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KLH32_002764"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBE0139528.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="KLH32_002765"
     CDS             complement(4991..7225)
                     /locus_tag="KLH32_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBE0139529.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KLH32_002766"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KLH32_002766"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBE0139530.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="KLH32_002767"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="KLH32_002767"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBE0139531.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KLH32_002768"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KLH32_002768"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE0139532.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="KLH32_002769"
     CDS             complement(12980..14320)
                     /locus_tag="KLH32_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE0139533.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KLH32_002770"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KLH32_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBE0139534.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="KLH32_002771"
     CDS             complement(16108..16557)
                     /locus_tag="KLH32_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBE0139535.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KLH32_002772"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KLH32_002772"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBE0139536.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="KLH32_002773"
     CDS             complement(17357..17686)
                     /locus_tag="KLH32_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBE0139537.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KLH32_002774"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KLH32_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBE0139538.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="KLH32_002775"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="KLH32_002775"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBE0139539.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="KLH32_002776"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="KLH32_002776"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBE0139540.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5670 c         5073 g         5286 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc atgccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatat
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DAEEDS010000067        20860 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEEDS010000067 DAEEDS010000000
VERSION     DAEEDS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20860
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.067"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            410..1240
                     /locus_tag="KLH32_002777"
     CDS             410..1240
                     /locus_tag="KLH32_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBE0139541.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="KLH32_002778"
     CDS             complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="KLH32_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBE0139542.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2369..3829)
                     /locus_tag="KLH32_002779"
     CDS             complement(2369..3829)
                     /locus_tag="KLH32_002779"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBE0139543.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="KLH32_002780"
     CDS             complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="KLH32_002780"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBE0139544.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5434..6777
                     /gene="gntP"
                     /locus_tag="KLH32_002781"
     CDS             5434..6777
                     /gene="gntP"
                     /locus_tag="KLH32_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBE0139545.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="KLH32_002782"
     CDS             complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="KLH32_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBE0139546.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="KLH32_002783"
     CDS             complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="KLH32_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBE0139547.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="KLH32_002784"
     CDS             complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="KLH32_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBE0139548.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9006..11642)
                     /gene="fimD"
                     /locus_tag="KLH32_002785"
     CDS             complement(9006..11642)
                     /gene="fimD"
                     /locus_tag="KLH32_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBE0139549.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11709..12434)
                     /gene="fimC"
                     /locus_tag="KLH32_002786"
                     /pseudo
     CDS             complement(11709..12434)
                     /gene="fimC"
                     /locus_tag="KLH32_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12471..13010)
                     /locus_tag="KLH32_002787"
     CDS             complement(12471..13010)
                     /locus_tag="KLH32_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0139550.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13075..13623)
                     /gene="fimA"
                     /locus_tag="KLH32_002788"
     CDS             complement(13075..13623)
                     /gene="fimA"
                     /locus_tag="KLH32_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBE0139551.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14104..14700)
                     /gene="fimE"
                     /locus_tag="KLH32_002789"
     CDS             complement(14104..14700)
                     /gene="fimE"
                     /locus_tag="KLH32_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBE0139552.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15178..15780)
                     /gene="fimB"
                     /locus_tag="KLH32_002790"
                     /pseudo
     CDS             complement(15178..15780)
                     /gene="fimB"
                     /locus_tag="KLH32_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17234..17951
                     /gene="nanC"
                     /locus_tag="KLH32_002791"
                     /pseudo
     CDS             17234..17951
                     /gene="nanC"
                     /locus_tag="KLH32_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
     gene            17971..19077
                     /gene="nanM"
                     /locus_tag="KLH32_002792"
     CDS             17971..19077
                     /gene="nanM"
                     /locus_tag="KLH32_002792"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBE0139553.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19142..20122
                     /locus_tag="KLH32_002793"
     CDS             19142..20122
                     /locus_tag="KLH32_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBE0139554.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            complement(20591..>20860)
                     /locus_tag="KLH32_002794"
     CDS             complement(20591..>20860)
                     /locus_tag="KLH32_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0139555.1"
                     /translation="TLATLERLLSLLSAFEVVVWMTDGWPLYESCLKGKLHVISKRYT
                     QRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         5549 a         4986 c         4738 g         5587 t
ORIGIN      
        1 tacgtagatg aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt
       61 ccgcataatc agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa
      121 catatcaaat acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc
      181 aatatcaaat tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa
      241 tgagcgtttt ttgatacttt agaatgtttc ctacccttta atatcactgt gtagagttga
      301 tatcactaca acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat
      361 ttatctattt ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc
      421 attaagcaat gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa
      481 agtcgacagt gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta
      541 tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat
      601 tagaaaactt aatattggca aacagtcctg gccatttaat tatgtgacag atacatgcag
      661 agaattcaat ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc
      721 agctttagac gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc
      781 agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac
      841 agttcatgaa ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat
      901 aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag
      961 aattgatatc tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt
     1021 cgcccttgcc gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat
     1081 agaagcctat cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg
     1141 tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt
     1201 tgaattaaaa aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac
     1261 taatattcag gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat
     1321 tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag
     1381 tcaggcgtcg actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc
     1441 gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc
     1501 cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc
     1561 caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg
     1621 attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt
     1681 tgcttcagca atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct
     1741 ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg
     1801 ggtagcctgc agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa
     1861 cagctcaaaa ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct
     1921 gcctgagcta tcaagcacat agataccggc accccggcgc acttccacca gccctttgat
     1981 ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat
     2041 ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg
     2101 gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt
     2161 gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg
     2221 cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag
     2281 ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga
     2341 ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc
     2401 gttcgcacag ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca
     2461 gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat
     2521 actgcgcgtt aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac
     2581 cctgctcatc cacgccctgg gtgtaaccca tccagccagc cacgcccagc gccaggtgac
     2641 gccagctgcc gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact
     2701 tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac
     2761 gctcgatcag cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg
     2821 gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg
     2881 tgtcggcaat ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc
     2941 cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgtgc
     3001 ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga
     3061 acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta
     3121 aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt
     3181 tctcttcaat ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga
     3241 ccttcgccac atggccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca
     3301 gccctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg
     3361 cagtcgggtt ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg
     3421 ccgcatcagc gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac
     3481 gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca
     3541 tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca
     3601 gttgctgttt tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt
     3661 cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt
     3721 gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat
     3781 cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca
     3841 gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc gtagccatgc
     3901 cggtgggtat taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc
     3961 ggccaggcct ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc
     4021 gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc
     4081 tttacgacgg tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc
     4141 gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag
     4201 atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc
     4261 acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc
     4321 aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg
     4381 cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat
     4441 cgctttcagg aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag
     4501 ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc
     4561 aatgatgtta cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc
     4621 tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg
     4681 catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag
     4741 cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt
     4801 gcgaatgcca cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc
     4861 atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca
     4921 caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac
     4981 ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac
     5041 atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt
     5101 tcctctttca catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt
     5161 atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt
     5221 tctacctttt ggtaagacat gtcaaccaga attctgaaat tggttaactg catcgcaaga
     5281 atttcactct catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc
     5341 agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa
     5401 taacaacggt tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg
     5461 gtattcggca ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg
     5521 gctttgttgg tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg
     5581 ctgcacacca tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg
     5641 ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat
     5701 acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcctt
     5761 attttcggtc tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt
     5821 gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct
     5881 gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct
     5941 tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta
     6001 atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca
     6061 ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt
     6121 ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg
     6181 gtaaaagata cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg
     6241 tttattgcga tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag
     6301 tgggtgatga acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc
     6361 ggtgcgggtg gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc
     6421 atgttgatgt cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta
     6481 attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt
     6541 gctgcaatcc ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta
     6601 ctggcgacgg ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg
     6661 ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     6721 ctggtcaact ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag
     6781 aacaccagag cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg
     6841 gacagtcccc tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg
     6901 cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg
     6961 tgcaggtttt gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt
     7021 aattttttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     7081 cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta
     7141 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     7201 cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     7261 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     7321 gaggaattgg cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa
     7381 catcacaacc gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact
     7441 gaaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac
     7501 cagctttaat cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     7561 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg
     7621 gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag
     7681 ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa
     7741 agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt
     7801 ttacataaac attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac
     7861 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag
     7921 ctcgtttcat tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact
     7981 gcctgaatgg ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg
     8041 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     8101 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta
     8161 taatatccgg tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     8221 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata
     8281 agactgaaag aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac
     8341 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     8401 cttgcgagcg ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc
     8461 tccagttact gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca
     8521 ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc
     8581 agggtcgtcc acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca
     8641 ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg
     8701 ttggcattgt ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta
     8761 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta
     8821 aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca
     8881 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg
     8941 cgacttaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     9001 cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc
     9061 agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt
     9121 cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc
     9181 tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc
     9241 gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga
     9301 gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta
     9361 tccagcgcca ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc
     9421 cagtcggtac gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta
     9481 acaagcacca ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat
     9541 accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata
     9601 ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta
     9661 ccatcgcctc ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc
     9721 aacgtaccgt atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg
     9781 tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa
     9841 ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc
     9901 aggctatagc tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat
     9961 tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt
    10021 gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg
    10081 aggttgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg
    10141 ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa
    10201 taacggtaac ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga
    10261 aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc
    10321 atatccacag acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca
    10381 cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg
    10441 agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct
    10501 cctgccgtaa tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag
    10561 ggtacggtaa aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca
    10621 ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta
    10681 taaatgccat atccattttg tttaatagtg acctgtgcag taccacgagc aataccgtag
    10741 atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt
    10801 gcgccgcgaa agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc
    10861 agccgggaac gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta
    10921 tttttgctac ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa
    10981 cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc
    11041 ccaatccgat tctgtacact atttccgctg aaattataat tgagcaatcc ggcattaata
    11101 ccgggatccc ataactcagg aggaatataa ccacgcgcgc gattactcat aaatgcctga
    11161 gggatcgtca ggttcagtcg ctgctgacca acatccagat gcgcagtagc gtcttggacc
    11221 attgtggtta atggcacaca ggcatcatcc gccagcagat tcataccggc gacagaagcc
    11281 gtattcagcc ccatactggc gagttgcgcg cgtgtcaggc agggaacaat cccttgttca
    11341 ctgtcgcccg tattaaatgt gacatcacgc gttgccatat aaccattatt caaatagata
    11401 tcgacgcgat acgtccctgg cggtaattct tgcccatttt caaaacgcga taaatcggcc
    11461 acagcctggg gatcatccgc taaaaagcgc agattaaaat agaggtcggc agatgacaaa
    11521 ggtgcctgtg cggcaaaagc acaggcgaca acgagtcgga caaaaaaacc agccaaacga
    11581 tgcttacgaa tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac
    11641 attattcctc cggtttaaaa tcacccggca gtcaattctt tttattcagg cgaaaaattc
    11701 cccctgcgtt attccattac gcccgtcatt ttgggggtaa gtgcgccata atcatttatt
    11761 gttcggtaag taatattgct tcctgcatca gaaggcaatt taaccgtgct ttcgcccatt
    11821 ggaggcacca atgcattttc aagaacccgg gttccggcat tcaactctgt taccgtcagg
    11881 taatagggtg tcgggttaat cagcgtcaga gaattcgcgc tacgacgaaa tcttaatttt
    11941 tctgcggcct gatcgggtgg caacgctaat ttagccgggc gatagtacag tttaatgcgg
    12001 ctgataattg cgagctgtag catattctca gtcaattttg atttatccat tgacggaatc
    12061 gctttaacgt tcatccagaa taaactttcc cggtcctgtg gcaattggtt atttgttgca
    12121 tcaagaatac gtaaggtatt ctcttttttt cccttcatcg caaacagagg aggcgtcacg
    12181 ataaaacgac catcctttac accatcggca ttttccaccc atgattgaat taaataggta
    12241 ctattttcat cattatttgt cacggcaagt tgcacttgtt tttaccctgc cggataaatt
    12301 acgcgagtcg cacctaaggc cactcctgct tcagcgcgtc cggcaaccat cattgccatg
    12361 aacatcagga tacctgccag caagcagaat gttatttcct gcgatttcct tacattgacg
    12421 tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa ttactgatag
    12481 gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg cccggtagca
    12541 cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt ccagtttgct
    12601 ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac accaatattg
    12661 gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc atccgcgaca
    12721 ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg taaatgaata
    12781 acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact gctgatttgc
    12841 cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc cgcaataatg
    12901 acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt tccggccaga
    12961 gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat cgttattttt
    13021 atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg taggttattg
    13081 atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct cgccgattgc
    13141 ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg tttgtgcact
    13201 aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct gcacgccaac
    13261 gtttgttgca ctacctgcag cagaactctg cagagccagt acatcagtac gcgtagcatc
    13321 aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat cgcaatcatt
    13381 cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca ggctagcggt
    13441 acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg cgcaagcggc
    13501 gttaacaact tcccctttaa agtgaatggt cccaccattt accgtcgtag tatcggccag
    13561 agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag ttttaatttt
    13621 catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac
    13681 tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa aagatgaaac
    13741 atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt cagtttattt
    13801 gttggcttaa tattctatta ttatctttat ttatagattt ttatattgca tgaggtagtt
    13861 tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt tacatatagc
    13921 ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata gttagatgct
    13981 ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat ttatgatatg
    14041 gacagtttgg ccccaattgt cttgtgttta tttgtttttt aaatttatta tcaataagtt
    14101 aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt cccataatcc
    14161 ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat ttcgattccc
    14221 gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat aaccgcaagc
    14281 atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac cggcatcgcg
    14341 aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatagc
    14401 gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt ccacggcttc
    14461 acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt
    14521 tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt cactaatacg
    14581 catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta
    14641 acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac
    14701 aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg
    14761 tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg ctatcagtca
    14821 attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc tattttcatt
    14881 gatttgattc aatataacaa tagattacag aggattaact tccagttact taaaaaataa
    14941 agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa tgattttgtt
    15001 tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa cttaatgttt
    15061 attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt taatcatttt
    15121 tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca actatctcta
    15181 taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa aacgccctgc
    15241 attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat aatcctagat
    15301 aagtcgcgta tctattccca tattcgccaa agcaaaacca cacgaatagc gtaacatgtg
    15361 cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa tatggtaaaa
    15421 ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc tctcagcatg
    15481 cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt tgttcaatag
    15541 cgggtgcgtt gttgaaaagc ctttttttaa tcgatggata tatatacact ttgctttaag
    15601 atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat gaataaaaca
    15661 aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg ctttaaggag
    15721 tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct tattcctcat
    15781 cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat aatccaacat
    15841 ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg taagtggacc
    15901 agttcttctg agcgaactta aatggagtag cgatagttac ttataataac tttgccatac
    15961 aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga tacgaatcta
    16021 ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga tatgttctgt
    16081 aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttaaa ctagccgtct
    16141 caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca tggaacgcag
    16201 taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta ttcctgcctg
    16261 gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga tatgggggaa
    16321 cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc actacgactt
    16381 aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta agtgaaacat
    16441 catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag tgaccagtta
    16501 caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct gatggcgagt
    16561 aaacccttat tttcaccact ctcactgagt tagcttgcaa agctttcttt gtttagattc
    16621 taaataaatt atttataaaa catatagtta aaagaaaatc tttagttttt gaagaccacc
    16681 gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg acatattttg
    16741 tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga tctggtataa
    16801 caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat ctatggcaaa
    16861 catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga aaggaatatg
    16921 aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt gagtaagtga
    16981 tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac tcaggtattt
    17041 aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg agaccttata
    17101 caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg caaaaaaata
    17161 ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg tatcctacaa
    17221 atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac tgtgcttttc
    17281 gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc gtagtggaag
    17341 ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat ggtgggcaag
    17401 catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg ccgcacatca
    17461 atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc
    17521 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
    17581 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
    17641 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
    17701 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
    17761 aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc
    17821 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc
    17881 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
    17941 ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc
    18001 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
    18061 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca
    18121 gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc
    18181 cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt
    18241 ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac
    18301 aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt
    18361 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
    18421 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
    18481 atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
    18541 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
    18601 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
    18661 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
    18721 aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
    18781 aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag
    18841 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
    18901 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
    18961 tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg
    19021 gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc
    19081 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta
    19141 tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa
    19201 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag
    19261 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgacat gtcactttaa
    19321 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
    19381 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
    19441 acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg
    19501 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
    19561 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
    19621 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
    19681 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
    19741 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
    19801 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga
    19861 agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
    19921 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
    19981 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
    20041 tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt
    20101 gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc
    20161 tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg
    20221 aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata
    20281 aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga
    20341 ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg
    20401 ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat
    20461 agacgatgat gatatgctta atgatgttca aagaaattag gccattatta tattattgct
    20521 tgcattttct ttcattccta aaagcacccg ccattaatcc caggcgcatt atgcaggtaa
    20581 tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc ttgtcatgca
    20641 gctccaccga ttttgagaac gacagtgact tccgtaccag ccttgccaga tgttgtctca
    20701 gattcagatt atgtcgctca atgcgctgag tgtaacgctt gctgataacg tgcagctttc
    20761 ccttcaggca tgattcatac agcggccagc catccgtcat ccataccacg acctcaaagg
    20821 ccgacagcag gctcagaaga cgctccagtg tggccagagt
//
LOCUS       DAEEDS010000068        20841 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEEDS010000068 DAEEDS010000000
VERSION     DAEEDS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20841
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.068"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(23..1021)
                     /gene="fbp"
                     /locus_tag="KLH32_002795"
     CDS             complement(23..1021)
                     /gene="fbp"
                     /locus_tag="KLH32_002795"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBE0139556.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1197..2570
                     /gene="mpl"
                     /locus_tag="KLH32_002796"
     CDS             1197..2570
                     /gene="mpl"
                     /locus_tag="KLH32_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBE0139557.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2653..3204)
                     /locus_tag="KLH32_002797"
     CDS             complement(2653..3204)
                     /locus_tag="KLH32_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBE0139558.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3298..4650
                     /gene="pmbA"
                     /locus_tag="KLH32_002798"
     CDS             3298..4650
                     /gene="pmbA"
                     /locus_tag="KLH32_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBE0139559.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4833..5219
                     /gene="cybC"
                     /locus_tag="KLH32_002799"
     CDS             4833..5219
                     /gene="cybC"
                     /locus_tag="KLH32_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBE0139560.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="KLH32_002800"
     CDS             complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="KLH32_002800"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBE0139561.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="KLH32_002801"
     CDS             complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="KLH32_002801"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBE0139562.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8418..10073)
                     /gene="treC"
                     /locus_tag="KLH32_002802"
     CDS             complement(8418..10073)
                     /gene="treC"
                     /locus_tag="KLH32_002802"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBE0139563.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10123..11544)
                     /gene="treB"
                     /locus_tag="KLH32_002803"
     CDS             complement(10123..11544)
                     /gene="treB"
                     /locus_tag="KLH32_002803"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBE0139564.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11663..12610)
                     /gene="treR"
                     /locus_tag="KLH32_002804"
     CDS             complement(11663..12610)
                     /gene="treR"
                     /locus_tag="KLH32_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBE0139565.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12795..12848
                     /gene="mgtL"
                     /locus_tag="KLH32_002805"
     CDS             12795..12848
                     /gene="mgtL"
                     /locus_tag="KLH32_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBE0139566.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12989..15685
                     /gene="mgtA"
                     /locus_tag="KLH32_002806"
     CDS             12989..15685
                     /gene="mgtA"
                     /locus_tag="KLH32_002806"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBE0139567.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="KLH32_002807"
     CDS             complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="KLH32_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBE0139568.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="KLH32_002808"
     CDS             complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="KLH32_002808"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBE0139569.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="KLH32_002809"
     CDS             complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="KLH32_002809"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBE0139570.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="KLH32_002810"
     CDS             complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="KLH32_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBE0139571.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18178..18573)
                     /locus_tag="KLH32_002811"
     CDS             complement(18178..18573)
                     /locus_tag="KLH32_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE0139572.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="KLH32_002812"
     CDS             complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="KLH32_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0139573.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19489..20082
                     /locus_tag="KLH32_002813"
     CDS             19489..20082
                     /locus_tag="KLH32_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0139574.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20227..20679
                     /gene="tabA"
                     /locus_tag="KLH32_002814"
     CDS             20227..20679
                     /gene="tabA"
                     /locus_tag="KLH32_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBE0139575.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4963 a         5228 c         5521 g         5129 t
ORIGIN      
        1 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
       61 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
      121 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
      181 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
      241 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
      301 cgatataacg cgaggtatac gggcgattgg tggatttatc ttcttcctgg cagaatttaa
      361 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
      421 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
      481 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
      541 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
      601 ccggcgtgcc aacaggcgta acgcggcggt agatggagaa aatggtaccg acagagacgt
      661 taacatcgat gttggacgag ccatccaggg ggtccatcag caccacgtat tttgcgtgtt
      721 cacagccttc aaagacgaca atctcatctt cttcttcaga ggcaatcccc gcaacgatat
      781 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
      841 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
      901 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
      961 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     1021 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     1081 tcaatgttgc gctcaggtga atcgcgccag caaattgcgg attatcctga aatgcgtttc
     1141 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     1201 gcattcatat tttaggaatt tgtggcacat ttatgggcgg tctggcgatg ctggcgcgcc
     1261 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     1321 ttgagaagca aggcattgag ctgattcagg gttacgatgc cagccagctc gagccgcagc
     1381 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     1441 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     1501 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     1561 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     1621 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     1681 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     1741 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     1801 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     1861 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     1921 gtgagcaggg tcactggcag gcgaaaaagc tgaccaccga tgcttccgaa tgggaagttt
     1981 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     2041 acaatggcct gatggcgatt gcagcggctc gccatgttgg tgtagcgccg gcagatgccg
     2101 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     2161 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     2221 cggcgctgcg tggcaaagtt ggtggtacgg cgcgcattat tgctgtgctg gaaccgcgct
     2281 cgaataccat gaaaatgggg atctgcaaag acgatctggc accttcatta ggtcgtgccg
     2341 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     2401 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     2461 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     2521 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcttca
     2581 gcctgatata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     2641 caacaacgaa aattaccctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     2701 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagtgtacg
     2761 cagttgctga cgatcggcat cgggccacag attcaatact tcagcgatgg cgtcatcacc
     2821 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     2881 gtgacggttt ttcagcttat ccagcgcctg acgaataggc tctacgtcgc gctggcgcag
     2941 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     3001 ctcaatagcc gggcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     3061 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     3121 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     3181 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     3241 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     3301 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     3361 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     3421 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     3481 agtgatggcg cgctggggat cactgtttat caccagaacc gtaaaggtag cgcatcgtct
     3541 accgatttaa gcccgcaggc cattgcccgc accgtacagg cggcgctgga tattgcccgt
     3601 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     3661 ccgaatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     3721 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     3781 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     3841 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     3901 gagcgtgatt acgcctacac cattggccgc gcgatgagcg atctgcaaac gccagagtgg
     3961 gtcggggccg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     4021 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     4081 ggggcgatag cgggtggctc ggtttaccgt aaatctacct ttctgctgga ttcgctgggt
     4141 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     4201 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     4261 ggcatcctga ctcagtggct gctgaccagc tactcggcgc ggaaactggg gctgaaaagc
     4321 accggacatg cgggcggtat tcacaactgg cggattgccg gacaagggct aagcttcgaa
     4381 caaatgctca aagagatggg caccgggctg gtggtgacgg agctgatggg ccagggcgtg
     4441 agtgccatta ccggtgatta ttcccgtggt gcagcgggct tctgggtaga gaatggcgaa
     4501 attcagtatc ccgttagtga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
     4561 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
     4621 ttgccggaga tgaaaatcgc cgggcagtaa aaaactggcg cgacctgccg cgctataaac
     4681 taaaaaatta cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg agggccgggg
     4741 agcgtactcg cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag gcagtgtgaa
     4801 ggatgaagtg taaataaaaa aggaagtgag caatgcgtaa aagcctgtta gctattcttg
     4861 cagtctcctc gttggtattc agttctgcgt cgtttgccgc tgatcttgaa gacaatatgg
     4921 aaaccctcaa cgacaattta aaagtgatcg aaaaagcgga taacgcggcg caagtcaaag
     4981 acgcgttaac gaagatgcgc gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc
     5041 tcgaagataa atcaccggac agcccggaaa tgaaagattt ccgccacggt ttcgacattc
     5101 tggtcggtca gattgacgac gcgctgaagc tggcaaatga aggtaaagta aaagaagcgc
     5161 aggctgctgc agagcaactg aaaacgaccc gcaacgccta tcaccagaag tatcgttaat
     5221 tcctcatttc cctgttgcct gcactcaggt aacagggaac ttatcatcgc aaatgatgca
     5281 ccacctgatt gctgctgccg cgccagatca gggaagggtc ttttaaatcc tgcacaaatt
     5341 tgccgtcgac cagcacgtta atcaaatcaa caacctgcat ttgcgcagcg ttgagttcgt
     5401 cgagtttata gcctgtccac acccagatgt ctttacccgg acactcggcg cggatgcgtt
     5461 ttaccagttt cagaatatcc ggcacgtttt gcggatgcag cggatcgccg ccggagaggg
     5521 aaatcccctg gcgtttgata cgagtgtcat tcagatcttt aatgatcttg tcttccattg
     5581 ctttggtaaa tggctgacca gaatttaccc gccatgtgct tttgttatag caaccggggc
     5641 attcatgaac acacccggag acaaacaggg tgcagcgagt gccggggccg ttgacgatgt
     5701 cgacaggata gtactgatga taattcatta tttcgcctgg cttatcagtc cgcgtgtagg
     5761 ctgcatccgg taatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacagccgt
     5821 tgccaaatgt aggccggata aggcgtttac gccgcatccg gcatttgctt agcgcagagt
     5881 gaagattaac ctatctgccc attccccaaa tgtttaacgc ggcgcttaac ttcttcctgc
     5941 ttaccagcgt taaacggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtt
     6001 accgacacac gggaggcgtc atggttacca catttcgggc aagtgaagcc tttgctggtg
     6061 cactcgaact caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg
     6121 taatacggta catgctgata gctgtaatcc cagacatctt ccagcgcttt caggttgtgc
     6181 tgaatgtttg gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc
     6241 gcttcaaagt cgatcttgtc gtacgggttc accttcttct ccacatcaag gtggaaactg
     6301 ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga
     6361 cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt aaagactgaa accataaccc
     6421 gtttcttctt tccactgatc cactgcctga cgcagacgtt caacaatggc gatacctttc
     6481 gcgcgaagct tctcgttgtc gtaaacatgc tcgccgctga acagcgcgtt aatggtttcg
     6541 tggatgccga tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaaca
     6601 tcatcgtcag cattcagacg tacgccacaa gcaccttcca tatagaggat cggggcgacg
     6661 cgcgctttca cgccttcgag acgagcgata cgggtcatca gtgccttacg tgccagcacc
     6721 agacgttcat ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata
     6781 cgcggcaggt tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg
     6841 ttttcatttt cccacacgcc gaggaagctg cggcagccca tcggggtttt aaacgaaccg
     6901 gtgacgttca ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac
     6961 tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt caggccatcg
     7021 cgaatcgcaa acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg
     7081 atacggttac gcaggatcga ttcctgaatc aggcgcgatt cccagctggt gcccagacca
     7141 aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac
     7201 tccagcgact ggaaggcatc gtagcactct ttgatggtac gagagttagc gtagccttcg
     7261 gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtaaca
     7321 aacggcgcca gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggcg
     7381 acctgagcaa taatctgcgc agttaccgcg gttgccgtag agatcgactt cggcggttca
     7441 atctcggcgt tccccatttt aaaaccctgg gtcagcatgc ctttcaggtc gatcaacatg
     7501 cagttgaaca tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat atcgccacgc
     7561 tcatgtgcct gcaccacgtc acgcggcagc aggtgctggc gtgcatagtg tttagccacg
     7621 atcccggcca gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg
     7681 ttgagtaacg aggcgttggt ctgctcgacc agaccacgga tctcctggtt caggcgaccg
     7741 cgtttttcac gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt
     7801 ttgtatggac ccgacatcag ctggttttca actgcggtct ggatctcgtt gatatccacc
     7861 tggttgcgcc cctgcatctg ctcgttgaca accgcggcaa cagtggcgca ataatcggca
     7921 tcatcgactt ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta
     7981 aacggcactt tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt
     8041 taagaacagg ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt
     8101 ccggtgcttt ccgcaatcgc catccgctta tccacaacgc cgcacctttt tcgtggggag
     8161 ctgtagtagc aattatagtc gattaataca acatattggg ttgggatgca ttttaaagtc
     8221 tatatatagt gctttgcatc aaggatgttt taactttttt tgatgtagct caaagtaaaa
     8281 agcagagcgt acggatgacg ggcgctacag cgttatgtaa attttttaat gaatttgctg
     8341 gttgaaaaat caacaaaaac aacatgctga cagacaaaac cccggaatga ccggcattat
     8401 cggggcttag ggaagattta cttctgtaac caccagacag cctcaaaagg ccgtaaattc
     8461 atggcacagg gttgtggtga ggcttcttcg taattatgca tcacaagctg ccagttgccg
     8521 cgcatttgcc ctggctgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag
     8581 gtttgcccct tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatcc
     8641 tggtaattgc cccatgtcag gatggcttct tgcttacgca gtgcgattaa cttttggtag
     8701 gtgtaaaaca cagaggagtc atcggccagc gcggcttcta cgttgatttg ttgatagtta
     8761 tcgcctaggc caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttgctccat
     8821 tgcatgggcg tgcggctgtt gtcacgggat ttactggcga ggattgccaa taactcgtcg
     8881 gcatcacgac catcgttgcg cagctcggca aacatattga ggctctccac gtcgcgatag
     8941 tcagtaatgc gcgtgaaatg cgggttggtc atgccaatct cttcgccctg gtagatatac
     9001 ggcgttccct gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat
     9061 tcaccttcat caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag
     9121 gcattccatg ccacgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc
     9181 acaaagtcag gtttagccag cgtccatttt tcaccaccgg gataatcgac cttcaggtga
     9241 tgaaaattaa aggtcatcga caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc
     9301 tcaaggctgg tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg
     9361 cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg
     9421 tcgccatcca ggtcttcagg gaaacgcggg tctttggaga tcagattcac cacatccagg
     9481 cgcaacccgt cgaccccacg atcggcccag aattcacaga cttttttcag ctctgcgcgt
     9541 accgctggat tctcccagtt gagatccgcc tgttctggtg caaagagatg caaatagtac
     9601 tgttcgcttt ccgcatgcca gcgccacgca ctaccgccaa attttgaacg ccagttgttc
     9661 ggtggcgttt ctggttcgcc atcgcgccag atataaaact ggcggtaagg gctttctttg
     9721 ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga
     9781 atgatacgaa tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg
     9841 ccgtaggtgg gatcaatcgc cgtatagttc gctacatcgt aaccgttatc gacctgggga
     9901 gagacataaa agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg
     9961 cgttggataa cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg aaaactcttt
    10021 ggataaatct ggtagataac gccgttttgc caccagtggg gaagattagt cattacgtta
    10081 ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa tgtccagcgt
    10141 gcccaggcgg tatttccgct gatagataaa cgaggtgagt acaatcggga tgatgatggc
    10201 gatagccatt gccagcgcaa atacctgcca gtagctcggt tgaatagaga gaattcccgg
    10261 caggccccct acgccgatac cattcgccat aacgccgttc aggccgcata gcaatcctgc
    10321 cagaccagaa ccaatcatcg cgcacagcat cgggaagcga tatttcaggt tgatgccgta
    10381 cattgcaggc tcagtgaccc caagccaggc ggagatagcg gcaggcacgg agatctcgcg
    10441 ttcattgtgc ttgcggctgg aaatgataat gcctatcacg gcggagccct gagcgatatt
    10501 cgacagcgca atcagcggcc acactggcgt gccacccatg ctttgaatca tctgcaaatc
    10561 aatagcaagg gtggtctggt gtacgccggt gatcaccagc ggcgcgtaca ggaagccaaa
    10621 caatgccgca ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac
    10681 gccatcgcca atcatgcgac caaacggacc aatcagcgca tgggcgagga acaccgcgag
    10741 gatcagcgaa cagacgggca ccaccaccag atagaggtaa tccggtacga tgcgtttaag
    10801 gcgagtttca ataacgccca gcgccagccc ggctaacagt gccgggatca cctgcgcctg
    10861 atagcccact ttggcgatgc tgaacatgcc aaagtcccac acttccggca gctgctgccc
    10921 gagcagataa gcgttcatca gctgggggga aaccagtgtc acgccaagca cgataccaag
    10981 gatcggcgtg ccgcccattt ttttcaccgc tgaccagcaa atcccgaccg gcaggtagaa
    11041 gaaaatcgct tcaccgatca accacagaaa atcgtagatc gtttgcaggg aagggtacat
    11101 ttgcgccagc gtctgaccgt tgctcatggg caaatcgccg atcacattgc gaaaaccgag
    11161 gatcaaaccg ccgctaatca acgcgggcag caacgggaag aagatctccg cgaaatgaga
    11221 gatcaactgc tcatgccatt tcatattttg ccgggcggct ttttttacct gctctttatc
    11281 aacctgcgcc tgtccggttg acgctattag tgcttgatag taatcaccca cgttggtgcc
    11341 aatcaccacc tgaaattgcc cggcattggt gaaacagcct ttcaccatgg ggagttgctc
    11401 aatttctttc ggtctggcat tggccggttg gttgaggaca aagcgcaggc gagtaataca
    11461 gtggctcacc gtcgcaatat tgccgcgccc gccgaccagt tcaatcaacc gatcgatatc
    11521 cgtttggttt attttgctca tcataaagcc ccatggcaga tgacattttt ggttggctgc
    11581 agaatatatt gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca gcgaagatca
    11641 caatttatcg ttcaggaaac gatcaggaca gggtggcggg gatgatgatt tgttgcggtt
    11701 cgctgcgccc ggttacctgt gcgatcaatt ggcaggccgc ctggcgtcca gcttcggcgt
    11761 agccaggatc tacggtgacg atctccggat gaaggaattt cattaacggc gtattaccta
    11821 cgctcgccag ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt gcgccaagag
    11881 ccagcgtgtc ggttgcgcac agtaacgcgg tagtttcagg cgtaatcact tttgctacgt
    11941 tttcatagcc ttgcttcata gcaagccctg gcagagcggc aacgggatgc agtttatgcg
    12001 ctttgcagaa cgccaggtag gcttcgtgac gtcgcttccc ggtcgtcacg tcactatgcg
    12061 gcacgccgag ataactgata ttgcgatgcc cctggtcata gagccgttgc atcagaatct
    12121 tgattgcccc ttcgtcgtca taacagaccg aagcaaagcc ttttgcgtca cgcgccagca
    12181 gaaccagcga tgactgccag tgggctaaca tttcttcggt tatgccggta aaaccgaaca
    12241 gcactacgcc gtcgatatta cgccgtttca gcacccccaa atgttcggca actaattgtg
    12301 gggaaaactg attttccatc atgattggat cgtaaccttg ttcatagaac gctggcagca
    12361 tggtttgaac ggcgagattt tctgacaacg aatccagacg ggtaacaatg atggcgacca
    12421 ctttatcgct ttgcccacgc atagcgcgcg cagagcggga aggggaaaat ccatgctgat
    12481 tcatcactgc ttcaacacgc tcgcgcgtgc gctgactcac gccgctttcg ttattcagca
    12541 cccgggaaac cgtagatttc cccacgccgc ttaagcgcgc gatgtctttg atggtcagcc
    12601 gattttgcat cctgttgtcc cataacgtgt tgtttaatta tttgagccta actttacccg
    12661 tgcattcagc aatgggtaaa gtctggttta tcgttggttt agttgccagc aggtattata
    12721 tcgccataaa tgctacgaat attattggat tctccttatt atttgcggcg cttttttcac
    12781 ttaccggagg ttatatggaa cctgatccca cgcctctccc tcgacggaga ttaaaacttt
    12841 tccggtaagc ccgtcttttc acggcgttac cggatgcgta aggccgtgac gttttaatgt
    12901 ccctgctcag ctttattacc ttcaggtaag gcttcgccac gcctgaagac atttctgtac
    12961 tgtttcagac agtgcggagg gactccttat gtttaaagaa atttttaccc ggttcattcg
    13021 ccatttacct tcccgtctgg ttcatcgcga tccattgcct ggcgcgcagc agacagtgaa
    13081 tacggcggtc ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga tgcccgaaga
    13141 agaattgtgg aaaacgttcg acacccatcc ggaaggatta aatcaggcgg aagtggaatc
    13201 tgcccgcgaa caacatggtg ataataaatt acccgcccaa caaccgtcgc cgtggtgggt
    13261 acatttatgg gtctgctatc gcaacccctt taatattttg ctcaccattc ttggtgctat
    13321 ttcttacgcc acggaagatt tatttgccgc aggtgttatc gcgctaatgg tcgctatttc
    13381 tacgttgctg aactttattc aggaagctcg ttccaccaaa gcggcagatg ccctgaaagc
    13441 gatggtcagt aataccgcga cggtactgcg agtaattaac gacaaaggcg aaaatggctg
    13501 gctggagatc ccgatagacc agctggtgcc cggcgatatt ataaaactgg cggcggggga
    13561 tatgatcccg gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg ctcaggcgtc
    13621 gttaaccggt gagtctctgc ccgtggaaaa agccgctact actcgccagc cggagcacag
    13681 caatccgctg gagtgcgaca ccctgtgttt tatgggcacc accgtggtga gcggcacggc
    13741 acaagcaatg gtgattgcta caggagccaa cacctggttt ggtcaactgg cggggcgtgt
    13801 tagtgagcag gaaagcgagc cgaatgcctt tcagcaaggg atcagccgcg ttagtatgct
    13861 gctgattcgc tttatgctgg tgatggctcc ggtggtgttg cttatcaacg gttacaccaa
    13921 aggcgactgg tgggaagcgg cactgtttgc gctttcggtc gcggtaggct taacgccgga
    13981 aatgttgccg atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc tgtcgaaaca
    14041 gaaagtgatc gtcaaacatc tggatgccat tcagaacttt ggcgcaatgg atattctgtg
    14101 cactgataaa accggcaccc tgacgcagga caaaattgtg ctggagaatc ataccgatat
    14161 ctccggtaaa accagcgaac gcgtgctgca tagcgcgtgg ttgaacagtc attaccagac
    14221 cggacttaaa aatctgcttg ataccgcggt gctcgaaggt acggatgaag agtcagcgcg
    14281 ttcgctggcc agccgttggc aaaaaattga tgagattccg tttgatttcg agcgtcgtcg
    14341 gatgtcggtg gtagtggcag aaaataccga acaccatcag ctggtttgca aaggtgcatt
    14401 gcaggaaatc ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg taccgctcga
    14461 tgacaccatg ctgcgtaaga ttaagcgggt tactgatacg ctgaatcgtc aggggctgcg
    14521 cgtggttgcg gtggcgacga aatacctgcc agcgcgtgaa ggagattacc agcgggcgga
    14581 tgaatccgac ctgatcctcg aaggatatat tgctttcctt gatccgccaa aagagacaac
    14641 cgctccggca ctgaaggcat taaaagcgag tggaattacc gtaaaaatcc ttactggcga
    14701 cagtgagtta gttgcagcga aagtgtgcca tgaagtgggg ctggatgcgg gagaggtggt
    14761 cattggtagt gatattgaaa cgctatctga cgatgaactg gcaaatctcg cacagcgcac
    14821 cacgctgttt gcccgcctga cgccgatgca taaagaacgc attgtgactt tactgaagcg
    14881 cgaagggcat gtggttggct ttatgggcga tggtattaat gatgcgcccg ccttacgcgc
    14941 tgcggatatc ggcatttctg tggacggcgc ggtagatatt gcccgtgaag cggctgatat
    15001 catcctgctg gaaaaaagcc tgatggtgct ggaagagggg gttattgagg gacgtcgcac
    15061 tttcgccaac atgctgaaat acatcaaatt gacggcgagt tctaacttcg gtaatgtgtt
    15121 cagcgtgctg gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt tacacttgct
    15181 tattcagaac ctgctgtacg atgtgtcaca ggtggcgatc ccgtttgata acgtcgacga
    15241 cgagcaaatt caaaagccgc agcgttggaa tccggcggat ctggggcgct ttatggtctt
    15301 cttcggaccg atcagctcga ttttcgatat tctgacgttt tgcttgatgt ggtgggtatt
    15361 ccatgccaac acgccggaaa cgcaaacgct gttccagtcg ggctggtttg tggtgggatt
    15421 actgtcgcaa acgctgattg tgcatatgat ccgtacccgc cgtgtgccgt ttattcagag
    15481 ctgtgcatcg tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg ggatcgcatt
    15541 gccgttttca ccgctggcca gttatctgca gctgcaggcg ctgccgttaa gctatttccc
    15601 gtggctggtt gcgattctgg cagggtatat gacgttaacc cagttggtga aagggttcta
    15661 tagccgtcgt tacggctggc agtaaagaat aaactgggca tggtggcccg gattcagatt
    15721 gcagacaaag tgagctttgt tcatgccgga tgcggcgtaa acgcccaatc cggcctacaa
    15781 attcaataaa ttgcaggaat cacgtaggcc tgataagcgt agcgcatcgg gcgattttgc
    15841 ttttgtcatc agacttaatc cgggcatgat agcccggatt tccatcaaga ttagcgacga
    15901 acagcgattg cttcgatctc aatcttcacg tctttcggca gacgggcaac ttcaacgcaa
    15961 gaacgtgccg ggaaagtggc gttgtgttcg gtgaagaagg cttcgtaagt ggcgtttacg
    16021 gttgcgaagt cgttcagatc tttcacgaac acagtggttt taacgatgtc gcccactttc
    16081 aggccagcgg cttcgacgat tgcttttacg ttatccagcg actgacgtgc ctgtgcagcg
    16141 acgtctgccg gtacttcacc agttttcgga tttaccggga tctgaccgga ggtgatgatc
    16201 atattgccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc attttccgtc
    16261 gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg gctcattata
    16321 gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt atgggaaaac
    16381 tctttttcac agtatttgca tttaagcgcg atgtcattgg cgcgttttcg cacggcaaag
    16441 ctggatgaaa ccggttcggc atggctgata cagttgctgt tcgggcagac cagcacattg
    16501 tcgatgcgct ctggcagact tgggcgcgat ttacccacca cttcatagtt gtcgatacgg
    16561 ttaaccgtgg cttgcggtgc atacaatgcc agttggtcta cttgctcttc actcaaaaag
    16621 gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcaa attcagacca
    16681 atggtgatgc gctgatctgt ttcggtcaac ttgaacagac tcaacagctt aaaaccgatc
    16741 tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg caatttatta
    16801 tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac cagtgccagt
    16861 aacgcctggc gagcgaaaat cccgttgcct gcttgctgga agtaccaggc gtgaggcgtt
    16921 ttatccacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac tttcatattg
    16981 gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac gttggcgtac
    17041 tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc tacttccgcc
    17101 atcacttctt caatagagct gtgcagactc caggcgatcc ctttttcatc gagcatatcc
    17161 agaatgtatt gcggcattgc cagcgcatcc ggcgcgataa agtaaaaacg gttgccgtcg
    17221 aacttcgcta gcgcctgggt cagggagtga acggtgcggc catatttcag gtcaccaacc
    17281 attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatggtgaa taagtccagc
    17341 aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg tacattgcca
    17401 gaaaattcgg tggctaggcg cgccgcacct tcctgcggat gacgcatcac tatcgcatcg
    17461 acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcctttttt acccagtgat
    17521 gtattggcgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat ggaagtttcg
    17581 aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac tttgtgcttc
    17641 aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac cagattaagg
    17701 tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg attagccatc
    17761 ttttatctcc tgacacctag gcaaaaaaaa gcccctcgat tgaggggctg ggaatgggtg
    17821 atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa gacaaaatgt
    17881 cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact catagccaga
    17941 agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt gaattaaaag
    18001 tcaaaatata tgcattttta ttttctatga ttctggaggg gaagtgtcgt tcggtatcaa
    18061 taattgcaga catttcctgc ttttttcttt ttcattttca cgcaatcaga aatgcatttt
    18121 attccattca tccgacttat agggcggagg gtgaaaacga acggctaaca ctattgctta
    18181 tagctcaggg atacgcgcta tcactttaat ttcaaaatca aagcctgcca gccaggtaac
    18241 acccaccgcc gtccagtttg gataaggtgg ggcgctaaat atttcatttt tcaccgtcat
    18301 gatgtcttca aattgttttt ccggatcggt atgaaagctc gtaacatcaa tgatatcgtc
    18361 aaaagtgcat cccgcagctg ccagggtcgc atgcaaatta tcaaaggcca gtctgacttg
    18421 ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc cggaaacaaa
    18481 cagcaaatcg ccggaacgaa tagccgcaga ataacgatgc tcagcataca gtgaatgtcg
    18541 gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat ttacgacatg
    18601 aacagaagat ttatatttat cgggagccgc ttttaggggg acgacgttag taaacaaaac
    18661 ccagacatca tggataatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    18721 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    18781 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcaac
    18841 atatcgcgca ttggcccgtt ggcgggatta acgtcggtat caattggtcc tggctggacg
    18901 acgttgatgg tgatccctcg cggtccaaaa tcacgggcca gcccgcgcgc catgccttgc
    18961 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    19021 acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc ttcaacagag
    19081 gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    19141 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    19201 ctcttacgaa cgacatcaat aacagcgtct ctgtcagcac tatctgtgaa tactgctgtc
    19261 gctccagtct cttgtgccag acgttcagcg gcatctttcg accccgcata ggtgaatcgt
    19321 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acgactgcca
    19381 ccgaggatga gaactgtctt accggtaaaa gcgcccataa ggactccttg atttattatg
    19441 taacatgcat tacaaaactg ttttaacttt ctgtcaacag gttttgtaat ggtcactaaa
    19501 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    19561 gcaaaagtgc tttttcacca aaaaggtttc gatgcagtaa gtgtctctga agttactgat
    19621 tatcttggta ttaacccccc gagtctctac gcggcttttg gcagtaaggc tgggttattt
    19681 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    19741 gatgatcgtc cggtaggcga gtgcctggtt gaggtattaa aagaagcggc gcgcagatat
    19801 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gtattcatag tcatgatcca
    19861 caagcgcgtg atattgccat tcaatattat cacgccgcag aaacgaccat ttatgactat
    19921 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    19981 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    20041 gccctggcag gggaagcgat aaaaactctt ctcaaggagt gatgctggcc ttgatccgaa
    20101 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctgg
    20161 cggaattgat catcttttcg tataacgata gaaatgaaac attattttaa ttaaggagtg
    20221 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    20281 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcatgat
    20341 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gtacgaagct
    20401 cgccgtgcgg agtaccatgc ccgctatctt gacattcaga ttgtgttaaa aggtcaggaa
    20461 ggcatgacct tcagcacgca acctgcaggc gcgccggata ccgactggtt agctgataaa
    20521 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatcctaaa tgaaggtgat
    20581 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    20641 caggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    20701 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    20761 ttgtaatgct tagacgttct tattaactca aggagttcgt catgggcaaa atatcagatt
    20821 tgaattattc tcaacacatt a
//
LOCUS       DAEEDS010000069        20724 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEEDS010000069 DAEEDS010000000
VERSION     DAEEDS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20724
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.069"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            81..350
                     /locus_tag="KLH32_002815"
     CDS             81..350
                     /locus_tag="KLH32_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBE0139576.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="KLH32_002816"
     CDS             344..709
                     /locus_tag="KLH32_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE0139577.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KLH32_002817"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KLH32_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBE0139578.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="KLH32_002818"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="KLH32_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBE0139579.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="KLH32_002819"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="KLH32_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBE0139580.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="KLH32_002820"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="KLH32_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE0139581.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KLH32_002821"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KLH32_002821"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBE0139582.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KLH32_002822"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KLH32_002822"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBE0139583.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12404
                     /gene="malT"
                     /locus_tag="KLH32_002823"
                     /pseudo
     CDS             9674..12404
                     /gene="malT"
                     /locus_tag="KLH32_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="KLH32_002824"
     CDS             complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="KLH32_002824"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBE0139584.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="KLH32_002825"
     CDS             complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="KLH32_002825"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBE0139585.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14882..16480
                     /gene="rtcR"
                     /locus_tag="KLH32_002826"
     CDS             14882..16480
                     /gene="rtcR"
                     /locus_tag="KLH32_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBE0139586.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16462..17220)
                     /locus_tag="KLH32_002827"
     CDS             complement(16462..17220)
                     /locus_tag="KLH32_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBE0139587.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="KLH32_002828"
     CDS             complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="KLH32_002828"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBE0139588.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="KLH32_002829"
     CDS             complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="KLH32_002829"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBE0139589.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18628..20133
                     /gene="glpD"
                     /locus_tag="KLH32_002830"
     CDS             18628..20133
                     /gene="glpD"
                     /locus_tag="KLH32_002830"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBE0139590.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20402..20632)
                     /locus_tag="KLH32_002831"
                     /pseudo
     CDS             complement(20402..20632)
                     /locus_tag="KLH32_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4894 a         5698 c         5230 g         4902 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatac gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    10741 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    10801 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    10861 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    10921 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    10981 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    11041 atgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat caggatgaag
    11101 cggaacggct ggcaaaactg gcactggaag agctgccgcc gggctggttc tatagccgca
    11161 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    11221 tggcgctagt gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    11281 tatggagttt aattcagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    11341 gggaaacgca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa
    11401 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    11461 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    11521 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    11581 cccgtagcca gttgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    11641 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    11701 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    11761 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    11821 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    11881 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    11941 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    12001 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactt aatacgctgc
    12061 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    12121 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    12181 cagaactaat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    12241 actccggtta cagcaatgaa caaattgcgg gcgaactgga agtggcggca accaccatca
    12301 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gctgccgtgc
    12361 agcacgcgca gaaactgctg aagatgatgg gttatggggt gtgagtttag ccggataatg
    12421 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctgtt
    12481 tctatcaaac caaaccgcac cggcaagaaa cgctccacca ccgcgatatt ggtcagcaga
    12541 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggcagtacc
    12601 agttggtcgg cgagatattc ccccaccgcc gctgggcttg ccaggtagcg tttcacctct
    12661 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    12721 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    12781 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaaa tccccgccag tgtagcgatt
    12841 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    12901 atgttcccgc gctcgccaag ttgcaaggtg ctaaacgatg ccacgggcga gacttccgtt
    12961 gccaccacac cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    13021 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    13081 gacgggttat cggtgccgcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    13141 gcgggcagca ccgtttgcag caccagcgta caacttccgg cgctaccgat agcaaagcgg
    13201 taatcgccgc cgcgcacggt gccaggccgg aagaccagac gctgcgatcc cagctccgcg
    13261 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    13321 agcagccccg gtttcgcccg tccggctcga atgccagtga tggtaaatgg ctggccggtt
    13381 atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcacca
    13441 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggtg
    13501 tagataactt ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc
    13561 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    13621 atttggtctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    13681 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    13741 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcaccttta
    13801 cgcgtcacgt agatctcttc accaaagagc tgttcttttt gcacatagtt gtggtgacag
    13861 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    13921 aacgccgtta ccacattttc catcatcgca tcgcggttaa ggctggcgaa aagctgcgcc
    13981 caggccacgg ctcttaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    14041 tcacgcgacg gcaacgtctc aagttggtcc tgcatctctt tttgcgccag atcgataaag
    14101 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgc
    14161 tccgactcat caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt
    14221 ttatagttgt tagtgttaag gaaacgcgga tatttttgcg ttaaccactg ataaccggcc
    14281 tccagctcag cccatttcgc atcgacgtta aaaggcgggt tttcccatgc gcctttatca
    14341 cgtttacaac gcccggtagt acgcccgtgt ggtacggccg tttcaatcgc ttgacgcagc
    14401 tccgccaggt tttcaggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca
    14461 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    14521 ccaatggtgg aacctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    14581 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    14641 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    14701 ttctcttttc gttgtttgcg gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    14761 gcagattaat tttttaattt attgtttcta aaaagattat atctttatgt ccgtaaacgg
    14821 agatttcccg caaagccgat ttaccgataa tgaaatatcg ccttttataa ggatatctaa
    14881 gatgcgtaaa acagtggctt ttggctttgt cggtaccgtg ctggattatg ccggacgcgg
    14941 cagtcagcgc tggtcaaaat ggcgtccgtc actctgtata taccagcaag aatcgttggt
    15001 catcgatcga ctggaattat tgcacgatgc ccgctcgcgc tcgctatttg aaacgcttaa
    15061 acgcgatatc gccagcgttt cgccagaaac agaagtggtg ggcgttgaga ttgaactgca
    15121 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga
    15181 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    15241 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaatcttc
    15301 aactccgcgc aaaaaagaac agccgcgcgg cccaggtgaa gtgacgatta tcgatctcga
    15361 tttaagccgt tataacgcca tcgccagccg ctttgccgag gaacggcagc aaacgctcga
    15421 tttcctcaaa tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    15481 cgaaaaagtg gcgattaaat cccgcgcgcc gattctgctt aacggcccga ccggcgcagg
    15541 taagtcattt ctggcgcgac gcatcttcga gttaaaacag gcacggcatc agtttagcgg
    15601 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgcgatgt cgacgctgtt
    15661 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggat tattacgcag
    15721 cgccaacggc ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc
    15781 aatgctgctg aaagctattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    15841 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    15901 aggcaaattc cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    15961 tctacgccag cgccaggaag atattgaacc gaacctggat tatgaagtgg agcgccacgc
    16021 cacactcact ggtgacagcg tgcgttttaa caccgaagcg cggcgtgcct ggctggcctt
    16081 tgcaacatct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    16141 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggtgg aagatgagat
    16201 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    16261 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    16321 ctgccgccag gcaaagtcgc tttctgctgc cggacgacag ctttttgatg tttcgcgcca
    16381 gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    16441 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac
    16501 ctgcatcacg cttactggcg gcggggcgtc ggtgtagacg gcatcaacca tgctgatgct
    16561 ccccatattg accatcgcat tacggccaaa ttttgaatgg tcaaccacca gcataacgtg
    16621 gcgcgagttc tcaataatgg cgcgcttggt acgaacttcg tggtaatcga actccagcag
    16681 cgagccgtcg ctgtcgatgc cgctgatccc cagaatgccg aaatcaaggc ggaactggga
    16741 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctacgta attcgccacc
    16801 ggcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacgt tgagattgtt
    16861 ggtgacaatg cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggcgtggt
    16921 gccgatatcg ataaacagcg tcgagccatt ggggatttgc tccgccactt tgcgggcgat
    16981 gcgctctttt tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact
    17041 ggaaggcagc gccgcgccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt
    17101 gaggtcgcgg cgaatagtct gcgggctgac ggagaagtgc tccaccagtt cctcggtact
    17161 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    17221 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    17281 ctaaacccac ggctaatccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    17341 caaaccatcc ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    17401 tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca
    17461 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcatatag ccgcttaaca
    17521 gggcgctgat aagcgtaatg acaattagct taccgctacc gaggcgtttt tccaccgcgc
    17581 cgccgagata ccaccaccaa agcaggttaa agaggatatg catcagcgag aagtgcatta
    17641 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    17701 gccataacat cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg
    17761 cgatcatcat cacccaggtc accggacctg cgcgttcacg caaggcagca aagaaaggat
    17821 aacggcgata atgcaggcca ctgccggtat ggcctgccag ccagctggcc gccagataac
    17881 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    17941 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct
    18001 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    18061 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    18121 gtacgccacc tctgcgggaa actgtcgctg ccaggcttca aagccgccgt caatgctata
    18181 gaccacatcg tagccctgtt gcagcagata ctgcgccgcc cctttgctgc tattgccgtg
    18241 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    18301 gtcgttggtt aaatggaaag cctgcaccgc atgccccata gcgaaactct gtggatcgcg
    18361 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    18421 acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    18481 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    18541 ttttcaatgt tacccaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    18601 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    18661 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    18721 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    18781 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    18841 gaagtgctgc tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat
    18901 cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg
    18961 ggtaaacgca ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta
    19021 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    19081 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    19141 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    19201 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    19261 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    19321 agccatattg tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa
    19381 gataaacgca ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc
    19441 gatgtggaat acaaaggtga tccgaaagca gtgaagattg aagagagtga aatcaattac
    19501 ctgctgaaag tgtataacgc gcactttaaa aagcagctaa gccgtgacga tatcgtctgg
    19561 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca agccattacc
    19621 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    19681 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    19741 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    19801 gaaggcgacc gcgacgatta cgccgctcgc ctgcgccgcc gttacccgtt cctgactgaa
    19861 tcgctggcac gtcactacac gcgcacttac ggcagcaaca gcgagctgct gctcggcaat
    19921 gcgggaacgg taagcgatct cggggaagat ttcggtcatg agttctacga agcggaactg
    19981 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctgtg gcgtcgcaca
    20041 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    20101 tatacgcagc agaagttgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt
    20161 tcaatctggc ctgagactga tgacaaacac aaaactgcct gatgcgctac gcttatcagg
    20221 cctacgtggt ttatgcaata tattgaattt gcatggtctt gtaggccaga taagacgttc
    20281 acgtcgcatc cggcatgaac aaagcgcact ttgtcaaaaa tctaacctac tttttaattc
    20341 agggaattac cgcaaagccc acgccatcat acccaacgta acaaaactca ggcacgttcc
    20401 ccactcgccc cgagaaaaat agcattaatg cgcccagcgt cagcataaaa atattgagca
    20461 gaaggtaaat tgttggcgta aagagtaaat tgcaaacctc aggaatagcg gcaagcccag
    20521 cccccagcat ggcaataacc gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag
    20581 tcattaccag cgaggtcaaa atccattttc tattttgcat tattctttcc attctttctg
    20641 aatggcgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
    20701 ggcgaaaaaa acgggaccct ttgg
//
LOCUS       DAEEDS010000070        20714 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEEDS010000070 DAEEDS010000000
VERSION     DAEEDS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.070"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="KLH32_002832"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="KLH32_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBE0139591.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="KLH32_002833"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="KLH32_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBE0139592.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="KLH32_002834"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="KLH32_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBE0139593.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="KLH32_002835"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="KLH32_002835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBE0139594.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="KLH32_002836"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="KLH32_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBE0139595.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYEALGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="KLH32_002837"
     CDS             complement(8218..9111)
                     /locus_tag="KLH32_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBE0139596.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KLH32_002838"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KLH32_002838"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBE0139597.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAEH
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KLH32_002839"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KLH32_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBE0139598.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="KLH32_002840"
     CDS             complement(10242..11474)
                     /locus_tag="KLH32_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBE0139599.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KLH32_002841"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KLH32_002841"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBE0139600.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KLH32_002842"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KLH32_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBE0139601.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="KLH32_002843"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="KLH32_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KLH32_002844"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KLH32_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBE0139602.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KLH32_002845"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KLH32_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBE0139603.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KLH32_002846"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KLH32_002846"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBE0139604.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KLH32_002847"
     CDS             complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KLH32_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBE0139605.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4982 a         5725 c         5033 g         4974 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcgcctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga agcgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg tgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa caacgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcta ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tagcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttagccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccaggctag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaagagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacgaacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat gtgc
//
LOCUS       DAEEDS010000071        20690 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEEDS010000071 DAEEDS010000000
VERSION     DAEEDS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.071"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            116..1171
                     /gene="phoE"
                     /locus_tag="KLH32_002848"
     CDS             116..1171
                     /gene="phoE"
                     /locus_tag="KLH32_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBE0139606.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1210..1611)
                     /gene="crl"
                     /locus_tag="KLH32_002849"
     CDS             complement(1210..1611)
                     /gene="crl"
                     /locus_tag="KLH32_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBE0139607.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1669..2913)
                     /gene="frsA"
                     /locus_tag="KLH32_002850"
     CDS             complement(1669..2913)
                     /gene="frsA"
                     /locus_tag="KLH32_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBE0139608.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3005..3463)
                     /gene="gpt"
                     /locus_tag="KLH32_002851"
     CDS             complement(3005..3463)
                     /gene="gpt"
                     /locus_tag="KLH32_002851"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE0139609.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3725..5182
                     /gene="pepD"
                     /locus_tag="KLH32_002852"
     CDS             3725..5182
                     /gene="pepD"
                     /locus_tag="KLH32_002852"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBE0139610.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5239..5853)
                     /locus_tag="KLH32_002853"
                     /pseudo
     CDS             complement(5239..5853)
                     /locus_tag="KLH32_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5915..6958)
                     /locus_tag="KLH32_002854"
     CDS             complement(5915..6958)
                     /locus_tag="KLH32_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBE0139611.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7204..7656)
                     /locus_tag="KLH32_002855"
     CDS             complement(7204..7656)
                     /locus_tag="KLH32_002855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0139612.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7666..8064)
                     /gene="yafO"
                     /locus_tag="KLH32_002856"
     CDS             complement(7666..8064)
                     /gene="yafO"
                     /locus_tag="KLH32_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBE0139613.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8067..8360)
                     /gene="yafN"
                     /locus_tag="KLH32_002857"
     CDS             complement(8067..8360)
                     /gene="yafN"
                     /locus_tag="KLH32_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBE0139614.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8412..9467)
                     /gene="dinB"
                     /locus_tag="KLH32_002858"
     CDS             complement(8412..9467)
                     /gene="dinB"
                     /locus_tag="KLH32_002858"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBE0139615.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9538..10323)
                     /gene="lafU"
                     /locus_tag="KLH32_002859"
     CDS             complement(9538..10323)
                     /gene="lafU"
                     /locus_tag="KLH32_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBE0139616.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10295..12007
                     /locus_tag="KLH32_002860"
                     /pseudo
     CDS             10295..12007
                     /locus_tag="KLH32_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12224..12721)
                     /gene="rayT"
                     /locus_tag="KLH32_002861"
     CDS             complement(12224..12721)
                     /gene="rayT"
                     /locus_tag="KLH32_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBE0139617.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12897..13418)
                     /locus_tag="KLH32_002862"
                     /pseudo
     CDS             complement(12897..13418)
                     /locus_tag="KLH32_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13802..14542
                     /locus_tag="KLH32_002863"
     CDS             13802..14542
                     /locus_tag="KLH32_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBE0139618.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14513..15280)
                     /locus_tag="KLH32_002864"
     CDS             complement(14513..15280)
                     /locus_tag="KLH32_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBE0139619.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15486..16064)
                     /gene="lpcA"
                     /locus_tag="KLH32_002865"
     CDS             complement(15486..16064)
                     /gene="lpcA"
                     /locus_tag="KLH32_002865"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBE0139620.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16304..18748
                     /gene="fadE"
                     /locus_tag="KLH32_002866"
                     /pseudo
     CDS             16304..18748
                     /gene="fadE"
                     /locus_tag="KLH32_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18791..19237)
                     /gene="ivy"
                     /locus_tag="KLH32_002867"
     CDS             complement(18791..19237)
                     /gene="ivy"
                     /locus_tag="KLH32_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBE0139621.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19418..20188
                     /gene="yafV"
                     /locus_tag="KLH32_002868"
     CDS             19418..20188
                     /gene="yafV"
                     /locus_tag="KLH32_002868"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBE0139622.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20298..20518
                     /locus_tag="KLH32_002869"
                     /pseudo
     CDS             20298..20518
                     /locus_tag="KLH32_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            20552..>20690
                     /locus_tag="KLH32_002870"
     CDS             20552..>20690
                     /locus_tag="KLH32_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0139623.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YT"
BASE COUNT         5054 a         5317 c         5317 g         5002 t
ORIGIN      
        1 tcagaatatc attaatctgt aatatatctt taacaatctc aggttaaaaa ctttcctgtt
       61 ttcaacgggg ctctcccgct gaatattcgc gcgttaatta aaatcaggaa tgaaaatgaa
      121 aaagagcact ctggcattag tggtgatggg cattgtggca tctgcatctg tacaggctgc
      181 agaaatatat aataaagacg gtaataaact ggatgtctat ggcaaagtta aagccatgca
      241 ttatatgagt gataacgcca gtaaagatgg cgaccagagt tatatccgtt ttggttttaa
      301 aggtgaaact caaattaacg atcaactgac tggttatggt cggtgggaag cggagtttgc
      361 cggaaataaa gcggagagtg atactgcaca gcaaaaaacg cgtctcgctt ttgccgggtt
      421 gaaatataaa gatttgggtt ctttcgatta tggtcgtaac ctgggcgcgt tgtatgacgt
      481 ggaagcctgg accgatatgt tcccggaatt tggtggcgat tcctcggcgc agaccgacaa
      541 ctttatgacc aaacgcgcca gcggtctggc gacgtatcgg aacaccgact tcttcggcgt
      601 tatcgatggc ctgaacttaa ccctgcaata tcaagggaaa aacgaaaacc gcgacgttaa
      661 aaagcaaaac ggcgatggct tcggcacgtc attgacatat gactttggcg gcagcgattt
      721 cgccattagt ggggcctata ccaactcaga tcgcaccaac gagcagaacc tgcaaagccg
      781 tggcacaggc aagcgtgcag aagcatgggc aacaggtctg aaatacgatg ccaataatat
      841 ttatctggca actttctatt ctgaaacacg caaaatgacg ccaataactg gcggctttgc
      901 caataagaca cagaactttg aagcggtcgc tcaataccag tttgactttg gtctgcgtcc
      961 atcgctgggt tatgtcttat cgaaagggaa agatattgag ggcatcggtg atgaagatct
     1021 ggtcaattat atcgacgtcg gtgctacgta ttatttcaac aaaaatatgt cagcgtttgt
     1081 tgattataaa atcaaccaac tggatagcga taataagctg aatattaata atgatgatac
     1141 tgtcgcggtt gggatgacgt atcagtttta atgaatattg ccggatgtga tgcatccggc
     1201 acatttcact cacgccgtta acttcaccgg ttcatcacga aaatcattcg ccggttccag
     1261 cttcagattc agcgtcgtca gcagttcacg cagcttctcg tgaaactcac gcagggtgtg
     1321 ctccagtcgt tcaaccactt cagtatcttt taccggaaca ctcttccagt cgcctgcttt
     1381 atcgaacaga ccaaactggt agctgtaggt aaaacgggat tcctgcgctt ccagctccat
     1441 ccaccagccc cagaattcac gcacttccgg tgccggtttc acgttgacgc atacagccag
     1501 acaatcgaaa aagaatcgat tatcttcgca cttaccttca cgaatatacg ggcctagtgc
     1561 ggtaaatttt ttgatcaatc tgctcttcgg gtgtccactc ggtaacgtca ttgctatctc
     1621 ctgttgtgat gcaactgttt taccaaattg gcaaaattta gcaaattttt aacacaagcg
     1681 tttttcgatc cagccggtga tttcctgaag acctttgtca aaattccgat acaccgggtt
     1741 aaatgggatc tctaataatt taccgtcagc agatgatgag gtgattaagc gtgagtcctc
     1801 ttccgggctg aacggatcgt tcttccagta gcctgataac attggcgttg ggcagcgacg
     1861 ccccagcaat ccttgcactt ttaatgaata gcgattcagc tccacgcgta acgcttcgtc
     1921 ggaagcatca tgcatcccca aacgactcgc cagaacgtca agatacattt ccggcacctg
     1981 ttgctggcac ttaaaatcac tcaacagggt atgaactacc ggaccaagac aggcaaccgc
     2041 tttcagacgc ggcgattcaa ggtatgccag acgcacggca acgttagcgc cgaaacggaa
     2101 accaaaggcc gcgacgcgag tgtgatccac ccacggtacg ttaggcagcg cctttaagac
     2161 gtgctgatgc aacaggctgg agtcctgggt gagcttccat tttgaagaaa agcccaccga
     2221 cggcatatca atagtcagca tcgcaatgcc gcgcggcgca aaataacgtt catacaggct
     2281 gtaatagtcc gtctgcatcg catccagacc accacacatt aatactgtcg ggaacgggcc
     2341 atcgcctttc ggcatatgca aaaagccggt gatgggcgca ccgccgggta cggtaaactc
     2401 catctgccgc atcgtgcccg gcagacgctg ggcggcctct tcataggcgc ggtttgataa
     2461 agcctgcgct tgctcggcca ggtcatctcc tttcagatga ggataggcgg caatgttgta
     2521 caacgtagcc gcatgcagcc agtgtcgacc actgagtttc ggatcttctt ccgtacaggc
     2581 tttttgttgc cacaccatcg cctgggtggc ccactcataa atccagttgc caccacggta
     2641 gccaatcacg gtatcgtata aatcatcgtc ggtacgttca gcatcgctca tcacaatacg
     2701 tgcctggacg tcgaggattt cacgtgggtc aataccgcgc cagatccaca tcagacggtt
     2761 aatcatgcga taccagtgag ggatggtttt accatcaaga gccgactgca caggcggttg
     2821 tgcgccgtga ttaaagcggc ggactagcgt cgaggtttca ggatgtttaa agcggggttt
     2881 gaacagggtt tcgctcaggt ttgcctgtgt catggatgca gcctccagaa tacttactgg
     2941 aaactattgt aacctgcctg aagttaaaaa gaacaacgcc cggcaatgcc aggcgttgaa
     3001 aagattagcg accggagatt ggcgggacga atacgacgcc catatcccac ggctgttcga
     3061 tccaggtatc ttgcgggata tcaacaacat agtcatcaac cagcggacga ccagccggtt
     3121 ttgcgaagat ggtgacaaag tgcgcttttg gatacatttc acgaatcgca acagcagtac
     3181 caccggtatc caccaggtca tcaataacga tgaagccttc gccatcgcct tctgcgcgtt
     3241 tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct ggaaatacaa acggtatcga
     3301 catgacgaat acccagttca cgcgccagta acgcccccgg taccagaccg ccacggctta
     3361 cggcaataat gcctttccat tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat
     3421 ggatctgcaa catgtcccag gtgacgatgt atttttcgct catgtgaagt gtcccagcct
     3481 gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat
     3541 ctggcgcact aaaaaccagt atttcacatg agtccgcgtc tttttacgca ctgcctctcc
     3601 ctgacgcggg ataaagtggt attctcaata catatctcgc aagcctgtct tgtgttgaca
     3661 acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga caaggagact
     3721 taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt ttgccaaaat
     3781 ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca ttgttggttg
     3841 ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatcc tgattcgtaa
     3901 acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc ttacaggccc acctcgatat
     3961 ggtgccgcag aaaaataacg acaccgtgca tgacttcacg aaagatccta tccagcctta
     4021 tattgatggc gaatgggtta aagcgcgcgg caccacgctg ggtgcggata acggcattgg
     4081 tatggcctct gcactggcgg ttctggctga cgaaaacgtg gttcacggcc cgctggaagt
     4141 gctgctgacc atgaccgaag aagccggtat ggacggtgcg ttcggcttac agagcaactg
     4201 gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa tctacatggg
     4261 ttgtgcgggg ggtatcgact tcacctccaa cctgcattta gatcgtgaag cggttccagc
     4321 tggttttgaa accttcaagt taaccttaaa aggtctgaaa ggcggtcact ccggcggtga
     4381 aatccacgtt ggcctgggta atgccaacaa actgctggtg cgcttcctgg cgggtcatgc
     4441 agaagaactg gacctgcgcc ttatcgattt caacggcggc acactgcgta acgccatccc
     4501 gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa gtcgacgcac tgaaatctct
     4561 ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca gagaaagaga aaaatctggc
     4621 cttgttgctg gactctgtag cgaacgataa agctgccctg attgcgaaat ctcgcgatac
     4681 ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc cgcaattccg acgtggcaaa
     4741 aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc atgactgaca ataacgtaga
     4801 aattcactgc ctgatccgtt cactgatcga cagcggtaaa gactacgtgg tgagcatgct
     4861 ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg aaaggcgcat atcctggctg
     4921 gcagccggac gctaattctc cggtgatgca tctggtacgt gaaacctatc agcgcctgtt
     4981 caacaagacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc tgttcaaaaa
     5041 accgtatccg gaaatggaca tggtttctat cgggccaact atcaccggtc cacactctcc
     5101 ggatgagcaa gttcacatcg aaagcgtagg tcattactgg acactgctga ctgaactgct
     5161 gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt aatcgccttc
     5221 cggcagtttc atccttcatt atccttcgat aaaagccatc cctgtaaacg ttcgtcgcgg
     5281 gttgccacgt tcaatctggt gatggaacat tcgccgctgt gatttcagca ccgcgctatt
     5341 ttcctattgc tgctgatcca gcttccaggc aatcagcaat cgcgccagcc gtttgttagc
     5401 atgctggctg cgctctgact gaaccttcac gctaataccg gatgccaaat gcgtggcgcg
     5461 taccgccgag tcggttttat tgacatgttg accgcccggc cccgacgaac gcagcgtctc
     5521 ataccggatc gcatcggatt gctcctgctc atcagcggta aaacgcccaa tgcccagaaa
     5581 ccagttttta cgcccgtgat gtgggcgata cgggctcggg caaatccact gaatagtgcc
     5641 acaccagctt tcgcttaacg cccatgcgtt gtcgccatcc agagaaatca gcgccgaacg
     5701 cagcgtgtca gagtagcggc ctgtttctgt ttccagcacc gttaccgcga cgtcttgtcg
     5761 ggcagcttct ttaattagcc tgtccagcgc ctttttcact gcgagacaac attcttccgg
     5821 cccctgcgct gaggagagtt atagcaagat catccttttt tccctccact gtttttgagc
     5881 gttagcaccg gacgcagccg cgcgacagga ataattaatc cggcctgccc agacattgca
     5941 ccacactttc agccgattta taggcttgtg gcgcttcttc gaagatgagt tgtttatcgc
     6001 gacaaattac ccgactgcca agttcagtac gcgagagttg cgtcgctgtg tatttcgctg
     6061 ccagacgccc tttacactcg gtgcgccccc atttacgccc agccccatgc gccagcgaat
     6121 gcaatgtttc ctcactcctg acgggctgaa ccagcctgga gtaatcaccg cgtgaaccgg
     6181 gaataaccac cagaccacag tcatcaggcg tggcaccttt acgatgtaag accccttgtt
     6241 gatcaccgat tcgacacgcg ctaacaaagt tatgcgccac atccagaacc ggacttccgg
     6301 tggccttaac ctgttgcatt atgcgcaaag cgatcaggtg gcgattaaaa cgcgcaaacg
     6361 ccagcgcgtc gtcactgcct tcaggcaaac catgatgcga aaacgaggca atatgccgct
     6421 gtaaaataga ctgccctagc cccctcgagc cgctatgaac cagcagttgc agatgctgcg
     6481 catccagacc tgccagtgca aacagttcag cgttgatgat ttgatcaact tgttgcagtt
     6541 cagcgaaatg attgccacca ccgatagagc caagcgagct gcgccacgga tgctgtgcaa
     6601 atgctgacgg caggttttcc tccagccagc tttcttcagc aacgtcatcc aaagctgata
     6661 atcgcttttc aaacttatcg gcgttgtatt tgcgagcgag aatatctgtt tgccatagcg
     6721 ccatgccaca gccgatatca ttgccgacca gtgctgggta aaaacggcct gcggagaaga
     6781 acgctgcgcc aatcgggtag ccgcgtccgg ggtgtaaatc tggcatcccc actacgcgct
     6841 gcatgttggg taaatttgcc gtggtgtgta attgttggat cgctaaactc tcgatccaca
     6901 ggtcatcaga tgcgatggta aataccgcat cggataaggg acgaatataa ttgcccatat
     6961 accattccgt aaatagactc gtcatacttc aagttgcatg tgctgcgact tgaattattg
     7021 agagtaacaa ttaaaaaatc aggaaatggc agacgaatcc agggcaagaa aaaaagaatt
     7081 aatcctggaa aggtctgcaa agagtcattg ataaaggatt tgtcatgatt tacctccttg
     7141 cagtttgtgg aataagaaaa cgcgcacata ctaggcgggc gttattttca ttgcaagctg
     7201 gatttaatgt tgcggtttat aacgcatata aaaattaata aaccacgctc cccggcattc
     7261 aacgcgctgc tgcttaactg tctgaaaacc ataacgttca aaaaagggtt ttgcggttat
     7321 gcttgcgtcc accgtaagtt cggattcaga cttaataaaa ggttttaaca aagcgctggc
     7381 aaccccacgg cgggtgtatt cagggtcaac aaataacata tcgatatagt gttcaacgca
     7441 ggtaataaag ccgaccagtt ttgcattaat aactgcaacc cgcacttgtg atttcgcgag
     7501 tttctccttc cagcgagatt cgtcaatctg cgcccaggcg gcaatttgtt gtagtgaata
     7561 atgctgactg acggtcatcg taaccgctct aatgaaaata gcgcatagtt gctgaaaatc
     7621 gccaggatga taatctctta tttgtatgtt attcataata taaattcaaa aacgcatgcg
     7681 aaacgcttct gccatttttc caattttatg catttggttg ttatctcgag ccagtttatg
     7741 aggttcaggc ttcagaatgg caatgagcaa ccatgcttgc tcatcaaacg caccctgaca
     7801 atataccaaa tgcgcttcgt cattcgttct gctgaattgg cgcaactgtg gcggaaatgg
     7861 attattcaca tttgccagat gaatatgagc aactcgctca aatttgatta atggccaggt
     7921 aaacgagtcg tcgtagagtg catcgcgacc aaatatatct ggcaaaacac cgtcacgctt
     7981 ataggaaata aaatccgccg ttaacgcatc aagttcctct gctgtaagtt gcaggcgaat
     8041 aagttttgtt ttgaataccc gcatccttat tccttaaagt cattgaaatc atcatccgtc
     8101 atatcattaa gatattgttt agcgcggcgt gtaatttcct ctaatcttgc agcacttgga
     8161 cgaaagcgcg cctttatggg ctttttctcc tcttgttcag caagcatgtc cattaacgct
     8221 tcgaatgcgc tggcacttaa gagatatcct gcggggcgat tattagaaag aaccgcaact
     8281 ggttgatcaa taaagtattt agctgggttt ttacgtaact cagtgatatt gaccgatttt
     8341 tcagcgagaa ttcgatgcat acagtgatac cctcataata atgcacacca gaatatacat
     8401 aatagtatac atcataatcc cagcaccagt tgtctttcca tttgcgggtc aagcaacgtc
     8461 acatgcagcc ccaccagacg cacaccgcgc ccgccgcggc gttcatccca ggttttacgc
     8521 gcggtggcga ttaagtcagc tttattcagc cgcggccaga cgtgctcctg ggtggtctgc
     8581 tgaaaatcgt cgaactttaa tttcaccccc tggcgagcaa tcagtaaatc aggttttacc
     8641 tttgccagac ggcgttcaag ttccggatac agccgctcga taatcgcttc acattcagac
     8701 cagtgatgaa tatcttccgc catcgtgcgt tccacgccga cggattttcg taaccgttcg
     8761 ctgttaacgt cgcgctcgtc aatcccctga ctacgctccc acaaaatgcg gccaaatttg
     8821 ccaaagcgtt taagcagaat caccagatca cacttttgta catcaccgca ggtccgcagc
     8881 cccatcgctt ccagttttgc cgccgagact ttgccgacgc cggggatttt ttccagtggt
     8941 aaggtttgta aaaatgccgg aacttctgcc ggcgtaatca caaactgacc gttgggttta
     9001 ttcatgtcgg aggcgatttt ggcgagaaac tttactggtg ccacgcccgc agatgccgtc
     9061 agttgcagct cgttgaagat cgtctggcgg atttcctggg cgatgagggt cgcagaaccg
     9121 tggcaatgga cgctatcggt gacatcgaga taagcctcat ccagtgacaa cggttcaatg
     9181 cgcgaggtgt agcgcgagaa gatttcacgg atatgatttg aggcttcttt gtaggcgtca
     9241 aagcgccccg gaagcaaggt gagatgtggg cataatttga gcgccatccc agtaggcata
     9301 gcgctacgta cgccaaattt acgcgcggga taattggcgg tgctgatcac cccccgacgt
     9361 tcgcggctgc cgccaatagc aatagggata tcgcgcagag cgggattgtc gcgcatctcc
     9421 accgctgcga aaaagcagtc catatccaca tgaatgattt tacgcattgc tcacctctca
     9481 acactggtaa agtatacagt gatttcaggt tttgagaaat gcgtaaagat tcagcattta
     9541 gcgcattcgc tgcgaaagca cctgctgctt atccagcttt tgcaccagcg gctgcaccac
     9601 tttatcccca tgctgaccaa agtattgata cagcgtatcg gacgcacttt tggtcagcac
     9661 cataatctca atacgccgat tgcccgcgct ttgcggattc ttcgcgtcca gcagcatctg
     9721 gtccgccatt gcgcttacct gcatcacttt atcttccggc attccggcct cttccagcac
     9781 ccgacgagct gaaagcgcgc gatcgcccga aaggttccag ttgttgtaga tattgttttt
     9841 gtaggccatc gcatcggtat gcccggtaat aataattttg ttatagagcg agtcgaacac
     9901 tggcgcgagc tccaccagca aagttttaaa gaacggcata atctgcgcgc tgccgcgttc
     9961 gaacatattg cgattctggt cgtctttaat cagcacgcgt aatccctggg gaacaatctc
    10021 catctccaga ttcgcttcca tatgcgcatc acgggcaatg gtgttgatac tggttgccaa
    10081 ctcgccaagc tcggtggcgg atttcttctt cagcagcgcc gttttctcat tcacatcacg
    10141 cgcttttttc tccgtctctt ccggcaccgc cacggtggca ggttttggcg gatgagacgg
    10201 gctgatttta tttaacggcg acagcccacc accgttaaaa atcgactgcc cgtgcagcgc
    10261 ggcgataatg ctttcacgct cggatttact gacgctgttg acaatcaact ttatcgtcgt
    10321 caccaaagag gccgagcgta tttccgaggt ttctgcccgc tttaccctgg acgcgatgcc
    10381 cggcaaacag atggcgattg acgccgatct taacgccgga ttgatcaacc aggcgcaggc
    10441 gcaaacccgg cgtaaagatg ttgccagcga ggccgatttc tacggcgcga tggacggggc
    10501 atcgaagttt gtacgcggag acgccatcgc cgggatgatg attctggcga tcaacctgag
    10561 cggcggcgtc tgtatcggca tcttcaaata caacctgagc gccgacgccg ccttccagca
    10621 atacgtgctg atgaccatcg gcgacggcct ggtggcgcag atcccttccc tgctgctctc
    10681 caccgcggcg gcgattatcg tcacccgcgt cagcgacaac ggcgatatcg cccatgacgt
    10741 gcgccaccag ttgctggcaa gcccgtcggt gctctacacc gccaccggga tcatgttcgt
    10801 gctggcggtg gtgccgggga tgccgcatct gccgtttttg ctgttcagcg ccctgcttgg
    10861 ctttaccggc tggcggatga gcaaacgccc gcaggcggcg gaggcggaag agaaaagcct
    10921 cgaaacgctg acccgcacca tcactgaaac cagcgaacaa caggtcagtt gggaaactat
    10981 tccgctgatc gagcccatca gcttaagcct cggctacaag ctggtggcgc tggtggacaa
    11041 agcgcagggc aacccgctca cccagcggat tcgcggcgtt cggcaggtga tttccgacgg
    11101 taacggcgtg ctgctgccgg agatccgcat tcgggaaaac ttccgcctca agcccagcca
    11161 gtacgctatt ttcatcaacg gcattgaggc tgatgaagcg gatattccgg cggataaact
    11221 gatggcgctg ccctccagcg aaacctacgg cgagattgac ggcgtgctgg ggaacgaccc
    11281 ggcgtacggg ataccggtca cctggatcca gcctgcgcag aaagcgaagg ctctgaatat
    11341 ggggtatcag gtgatcgaca gcgccagtgt gattgccacc catgtgaaca agattgtgcg
    11401 cagctatatt cctgatttgt ttaactatga tgacatcacg cagttgcata accgtttgtc
    11461 gtcgatggca ccgcgcctgg cggaggattt aagcgcggcg ctcaattaca gccaattgct
    11521 gaaagtgtac cgggcgctgc tgaccgaagg cgtttccctg cgcgatatcg tcaccatcgc
    11581 caccgtgctg gtcgccagta gcacggtgac taaagatcat attctgctgg cggccgatgt
    11641 gcgcctggcg ctgcggcgca gcattactca tccgttcgtt cgcaagcagg agctgacggt
    11701 gtatacgctg aataatgagc tggaaaatct gctgaccaac gtagtgaatc aggcgcaaca
    11761 ggccggcaaa gtgatgctcg acagcgtgcc ggttgacccg aatatgctca accagttcca
    11821 gagcacgatg ccacaggtga aagagcagat gaaagcggcg gggaaagatc cggtgctgct
    11881 ggtgccgccg cagctacgcc ctttgctggc gcgttatgca aggttgtttg cgccggggct
    11941 gcatgtgctg tcgtataacg aagtgccgga tgagctggag ttgaagatta tgggggcgtt
    12001 gatgtaagaa tccggcatcg gtgcacaatt gccggatgcg gcgtaaacgc cttatccggc
    12061 ctaccgttca ggcacaagtt tttaggcatg ataagacgcg ccagcgtcgc atcaggcatc
    12121 tgcgcataac tgccggatgc ggcgtgaacg ccttatccgg cctaccgtta aagcacgatt
    12181 tgtaggccgg ataagcgtaa gcgcatccgg caccagcgca cagttataaa atgatacgct
    12241 ccccggcact taaatccgtt atgtctcccg cccaatcgat gggatataac cctcgcgcga
    12301 catcgcgatg gaacgttgag aatggccaat cactcacctg ctttacccag ccatgtttct
    12361 ctggatttat ataaatataa tcaacatgat ggcgataatc ttttgtattg cgaatggcat
    12421 gctcccaaaa acgcggttgc cagatatttt tcaatccgca agcatgggtg aactgctttt
    12481 taatctcccg ccagcgagag gaaaaatcat catcgctttc gggtaatgtc catatgcaat
    12541 gcatatgctc cggcaaaacg acccaggcgt taatttcaaa aggtctgtct cgcttaacgt
    12601 taataatggc gttacggagc gtctggaact gggtggtcag aagatggctt cgacgattgc
    12661 gtaaatttac cgtgaaaaac catgttcccc ccttgatgta ataacgacga tattccgaca
    12721 tagcgactcc tttttctcaa cggtaagagg aaaaggaaag gccgacaatg acgctgtgtt
    12781 atatcgggaa agcggctcgc aaaaacgcac agatttgtag gcatgataag acgcgtcagc
    12841 gtcgcatcag gcatccgtgc acaaccgccg gatgcggcgt aaacgcctta tccggcctac
    12901 aaaatcatct cttccgtcaa aatcctgcgc gcgcctaaaa aatggtcctg ccagaaaggt
    12961 tcggctaacc ggcttacccg aatggtttcc ccggtgcgca gtgactcaat aaactgaccg
    13021 tcgcccagat acacccccat atggtcggca atctcgcggc tgtggatatg gaaaaatagc
    13081 aaatctcccc ggcgcaggtc gttattcgcc acaatcgttg cccgacggta gtggtacatc
    13141 tcattggccg tgcgcgggag tttcgcctca aggatcttgt tgtaggcata aaaaactaac
    13201 ccactacaat cgaagccttg atcgggccgc gtgccgcccc agacgtaagg cttgccaagc
    13261 tgctgctcca ggcggtgaat cgccacttcg gtgatgttat gcaggtgatc gctgcttaaa
    13321 aagtggttgt tttccgccag cgcctgccag cgtctgtcac tcgccttgag cggcgcagga
    13381 aaccattctg gatgctgttt tatctgctcc cggttgtggt ggcgctggcg agtaaaggga
    13441 gcaaaacgca taacgaaaac acgcctggat gcaaaaatct gccgagcaag aacgaagaca
    13501 tgaaagacat aaaccggctg aatgaaggtg agaattttaa aagaccggag gagtaaacgg
    13561 gattattaat tgcttatttg catattacta ttgagatttc ataatattag gaaataaatg
    13621 atacatgcgc ttttcggact attgaaaact gcccctgtgc taaatttgta aacccagcgc
    13681 cgacaggctt gcgtaaaact cttttggtga taatgtgagc cttccaaatc cagactattc
    13741 cgatttggga tccattttgc cgtcctggcg tgtgtaaccg ttttatcaag gaataagcag
    13801 tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc ccgtgcgttt cgtttgccgg
    13861 tttgctgggc agcagtagct cgaccacgcc agtgagcaaa gagtataagc agcagttgat
    13921 gggatcccct gtctacatcc agatcttcaa ggaagaacgt acgctcgatc tttacgtcaa
    13981 aatgggcgag caatatcaac tgctcgacag ttataaaatc tgtaaatatt ccggcggctt
    14041 agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa gggttttata gcgtccagcg
    14101 taatcagtta aaaccagaca gccgttacta caaagcgatt aatattggat tccccaatgc
    14161 ttatgaccgt gcgcatggtt atgaagggaa atacctgatg atccacggcg attgtgtatc
    14221 catcggctgc tacgcaatga ccaatcaggg tattgatgag atcttccagt tcgttactgg
    14281 tgcgctggtg tttggtcagc cgagcgtgca ggtgagtatt tacccattcc gcatgaccga
    14341 cgccaatatg aagcgccata aatattccaa ctttaaggac ttctgggagc agctgaagcc
    14401 gggctacgac tactttgagc aaacccgtaa gccaccgacc gtttctgtgg ccaatggccg
    14461 ttacgtggtc agcaagccgt taagccacga agtggtgcag ccacaactgg catcaaacta
    14521 cacgctcccc gaggcaaaat aagtgccatt cgcctggcat aattttttgc caggtttcat
    14581 tgcccgtcag cggctgtgtc gcaatcaccg tgaccacatc attcggtgtg gtctgcgagc
    14641 tgaagtcgat ttccacatcc tgatccagca acgttgccac gccaaacggc gcgcggcggg
    14701 tgatccagtg taaattagtc gagcaatacg ccattacata gcgcccgtcc gaaagcagca
    14761 tgttgaaaac gcccttcgcc cgcagttcat ccgccagtga ggcgatatat ttaaacactg
    14821 ccgccatgtt gcccggcgtg cgcgggtaac gctgcgttaa tttatgcagc agccagcaaa
    14881 aggctttttc gctgtcggtt tcaccgaccg ggcggaagtt gccggtttcc agtgatttgt
    14941 agcccgtcag ttgtccgtta tgggcataag tccagttacg cccccataac tcgcgggtaa
    15001 atgggtgagt attttccagc gccacctcgc cccgattagc ctggcgaata tgagccacca
    15061 ccgaacagga ttttatcggg tagtcctgga caagtttggc gatgggggaa ttaaagctgg
    15121 gttgtggatc tttaaatgtg cgacagcctt taccttcgta aaaggtaatg ccccagccat
    15181 ctttatgtgg cccggttcca ccaccacgct gtacaagtcc ggtgaaacta aagcagatat
    15241 cggtagggac gttggcgctc atcccgagca attcgcacat gggtaacctc cacaacagcg
    15301 ggagcgcatg gaccccggcg aagattgatt taagaatttt caggtcggat gcggcgtaaa
    15361 cgaattattc ggcctacggt tcgggtacag atttgtaggc atgataagac gcgtcagcat
    15421 cgcatcaggc atcagcgcac aagtgccgga tgcggcgtaa acgccttatc cggcctacgc
    15481 cagacttact taaccatctc tttttcaatt aactggatca ggatatggat cactttaatg
    15541 tgaatctcct gaatgcggtc ggcataacca aagtgcggaa cgcgaatttc gatatccgcc
    15601 gtgccagcca ttttaccgcc gtctttaccg gtcagggtga tcactttcat ccccttctca
    15661 cgcgccgctg cgatcgcttt gatcacgttt gcagagttac cggaggtgga gatccccagc
    15721 agtacatcgc cttcgcgacc taccgcttca acgtagcggg agaaaatatc attgaaaccg
    15781 aaatcattac cgacgcagga aatatgacta acgtcagaaa tagcaatcgc cgggtagccc
    15841 ggacggtttt cacggtagcg accggtcaac tcttcggcaa agtgcatagc gtcgcaatgg
    15901 gaaccgccgt tgccgcagga aagcactttg ccaccggctt taaagctgtc tgctaacagg
    15961 accgccgcgc gctgaatggc gtgaatattg gcgtcatctt ttaaaaagtt agccagcgtt
    16021 tccgccgctt cgttcagttc gttacgaata agatcctggt acatgaggat atccttcagc
    16081 ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc aaaagcgaga agtacgggca
    16141 ggtgctatga ccaggacttt ttgacctgaa gtgcggataa aaacagcaac aatgtgagct
    16201 ttgttgtaat tatattgtaa acacattgct aaatgttttt acatccacta caaccatatc
    16261 atcacaagtg gtcagacctc ctacaagtaa ggggcttttc gttatgatga ttttgagtat
    16321 tctcgctacg gttgtcctgc tcggcgcgtt gttctatcac cgcgtgagct tatttatcag
    16381 cagtctgatt ttgctcgcct agacagccgc cctcggcgtt gctggtctgt ggtcggcgtg
    16441 ggtactggtg cctctgacca ttatcctcgt gccatttaac tttgcgccta tgcgtaagtc
    16501 gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg atgccgccga tgtcgcgcac
    16561 tgagaaagaa gcgattgatg cgggcaccac ctggtgggag ggcgacttgt tccagggcaa
    16621 gccggactgg aaaaagctgc ataactatcc gcagccgcgc ctgaccgctg aagagcaagc
    16681 gtttctcgac ggcccggtag aagaagcctg ccggatggcg aatgatttcc agattactca
    16741 tgagctggcg gatctgccgc cggagttgtg ggcgtacctt aaagagcatc gcttcttcgc
    16801 gatgattatc aaaaaagagt acggcggact ggagttctca gcttatgccc agtctcgtgt
    16861 gctgcaaaaa ctctctggcg tgagcgggat cctggcgatt accgtcggcg tgccaaactc
    16921 attaggcccg ggcgaactgt tgcaacatta cggcactgac gagcagaaag atcactatct
    16981 gccgcgtctg gcgcgtggtc aggagatccc ctgctttgca ctgaccagcc cggaagcggg
    17041 ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc atgggcgaat ggcagggcca
    17101 gcaggtgctg gggatgcgtc tgacctggaa caaacgctac attacgctgg caccgattgc
    17161 gaccgtgctt gggctggcgt ttaaactctc cgacccggaa aaattactcg gcggtgcaga
    17221 agatttaggc attacctgtg cgctgatccc aaccaccacg ccgggcgtgg aaattggtcg
    17281 tcgccacttc ccgctgaacg taccgttcca gaacggaccg acgcgcggta aagatgtctt
    17341 cgtgccgatc gattacatca tcggcgggcc gaaaatggcc gggcaaggct ggcggatgct
    17401 ggtggagtgc ctctcggtag gccgcggcat caccctgcct tccaactcaa ccggcggcgt
    17461 gaaatcggta gcgctggcaa cgggcgcgta ttctcacatt cgccgtcagt tcaaaatctc
    17521 tatcggtaag atggaaggga ttgaagagcc gctggcgcgt attgccggga acgcttatgt
    17581 aatggatgct gcggcatcgc tgattaccta cggcattatg ctcggcgaaa aacctgccgt
    17641 gctgtcggct atcgttaagt atcactgtac ccaccgcggg cagcagtcga ttattgatgc
    17701 gatggatatt accggcggta aaggcattat gctcgggcaa agcaacttcc tggcgcgtgc
    17761 ttaccagggc gcaccgattg ccatcaccgt tgaaggggct aacattctga cccgcagcat
    17821 gatgatcttc ggacaaggag cgattcgttg ccatccgtac gtgctggaag agatggaagc
    17881 ggcgaagaac aatgacgtca acgcgttcga taaactgttg ttcaaacata tcggtcacgt
    17941 cggtagcaac aaagttcgca gcttctggct gggcctgacg cgcggtttaa ccagcagcac
    18001 gccaaccggc gatgccacta aacgctacta tcagcacctg aaccgcctga gcgccaacct
    18061 cgccctgctt tctgatgtct cgatggcact gctgggcggc agcctgaaac gtcgtgagcg
    18121 tatctcggca cgtctggggg atattttaag ccagctttac ctcgcctctg ccgttctgaa
    18181 gcgttatgac gacgaaggcc gtaatgaagc cgatctgccg ctggtgcact ggggcgtaca
    18241 ggatgcgctg tatcaggcag aacaggcaat ggacaattta ctgcaaaact tcccgaaccg
    18301 cgtggttgcc gggctgctga atgtggtgat cttcccgacc ggacgtcatt atctggcacc
    18361 gtctgacaag ctggatcata aagtggcgaa gattttacaa gtgccgaacg ccactcgttc
    18421 acgtattggt cgcggtcagt acctgacgcc aagcgagcat aacccggttg gtttactgga
    18481 agaggcgttg gtggatgtga ttgccgccga cccgattcat cagcggatct gtaaagagct
    18541 gggtaaaaat ctgccattta cccgtctgga tgaactggcg cacaacgcgc tgacgaaggg
    18601 gctgattgat aaagatgaag ccgctattct ggtgaaagcg gaagaaagcc gtctgcgcag
    18661 tattaacgtt gatgactttg atccggaaga gctggcgacg aagccggtaa agttgccgga
    18721 gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc tccgtttctt
    18781 tatccgctaa ttatttaaaa ttaaagccat ccggatggtt ttccaggctg ccggtcaacg
    18841 ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc gttcacattc agccaggtga
    18901 gtttctcttg cgacgttttc tcatcaatag ccgagaacag ccccgtcatc tgattagatt
    18961 tctcggacca catcacagcg atacgttgcg agccacagtc atgcggtttg cacgcgctca
    19021 tcacctgata cgtctcatct cccaacgtta cggtttgtgc gggagtataa gtaccgcctt
    19081 tcatcaccca tgcaggtagc ttatgccctt gcaccatctg attaaacgca gctttggtgg
    19141 tttcgccctt tgcaaggctg ctaatggtta aatcatcctg cgccattgca ctggtggcga
    19201 tgaccagtgc ggcgactgtc gttattgcct taaacatcat tcctcccgag cttatcctgc
    19261 ccatgttatt aacctcctaa agcctggcga taacacgata atttgcaaat cagatatttc
    19321 caatatcagc atttgttatc aatttattct gcgctaaggc aactgcctga taaggtgtta
    19381 aagttataaa acccttcgca taaaggagtt gtacaccgtg cctagtttga agattacgct
    19441 tttgcagcaa ccgttggtgt gggtggatgg tcctgccaac ctgcgccatt ttgatcgtca
    19501 actggaaggt attaccgggc gcgatgtgat cgttctaccg gagatgttta ccagcggctt
    19561 tgccatggaa gcggcagctt cgtcgcttgc acaagatgac gtagtgaact ggatgacagc
    19621 taaggcgcag cagtgcaatg cgctgattgc aggcagtgtt gcattacaaa cggagtctgg
    19681 ttcggttaac cgctttttgc tggttgagcc gggcggcacg gtacattttt atgataagcg
    19741 tcatctgttc cgcatggcag atgagcatct gcattataaa gcgggcaatg cgcgagtgat
    19801 tgtggaatgg cgcggctggc gtattttgcc gctggtgtgc tacgacttac gttttccagt
    19861 gtggtcgcgc aatctcaacg attatgacct cgccctgtac gtcgccaact ggccagctcc
    19921 gcgctctctg cactggcagg cattgctgac ggcccgcgcg attgagaatc aggcgtatgt
    19981 ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg gtgacagccg
    20041 ggtgattaat ccgcaaggcg agattatcgc taccgccgac gcgcataagg caacgcgtat
    20101 tgatgcggag ctgtcgatgg tggcattgcg ggaatataga gaaaagtttc cggcatggcg
    20161 ggatgcggat gagtttaggt tgtggtgagt gggggttgtg tttagggccg ggggctgggc
    20221 tagattacga aagcccctaa cttaaacaca atattacgcc agaagtcacc atttccaaaa
    20281 cacagcgtcg ctgtaccgtg ggttatctgc ccaaccgcag cgacacgccg gtgatcaaaa
    20341 tcagcgccaa atggctgaga aaagcgggat ttgagatcgg cacgggcgtt accgtgaaga
    20401 tctcggaggg atgtttgatc ctgcttggga taacaacgaa gtacaggaac tgcgcaggga
    20461 actttatcag gttaaacagc cagttaaggg gatgcgggta atgctgccaa cttactgatt
    20521 tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc atttccatca gatgtccttc
    20581 ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc actgccggac atcagcgcta
    20641 tctctgctct cattgccgta aaacatggca actacagttc acttacaccg
//
LOCUS       DAEEDS010000072        20521 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEEDS010000072 DAEEDS010000000
VERSION     DAEEDS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.072"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="KLH32_002871"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="KLH32_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBE0139624.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="KLH32_002872"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="KLH32_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBE0139625.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="KLH32_002873"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="KLH32_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBE0139626.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KLH32_002874"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KLH32_002874"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBE0139627.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KLH32_002875"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KLH32_002875"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBE0139628.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="KLH32_002876"
     CDS             4449..4814
                     /locus_tag="KLH32_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBE0139629.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="KLH32_002877"
     CDS             4964..5482
                     /locus_tag="KLH32_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBE0139630.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="KLH32_002878"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="KLH32_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="KLH32_002879"
     CDS             6713..7195
                     /locus_tag="KLH32_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBE0139631.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KLH32_002880"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KLH32_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBE0139632.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KLH32_002881"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KLH32_002881"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBE0139633.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KLH32_002882"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KLH32_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBE0139634.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KLH32_002883"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KLH32_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBE0139635.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KLH32_002884"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KLH32_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBE0139636.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="KLH32_002885"
     CDS             10939..11730
                     /locus_tag="KLH32_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBE0139637.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="KLH32_002886"
     CDS             11751..13037
                     /locus_tag="KLH32_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE0139638.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KLH32_002887"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KLH32_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBE0139639.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="KLH32_002888"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="KLH32_002888"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBE0139640.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="KLH32_002889"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="KLH32_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBE0139641.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="KLH32_002890"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="KLH32_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBE0139642.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="KLH32_002891"
     CDS             complement(16985..17275)
                     /locus_tag="KLH32_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBE0139643.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KLH32_002892"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KLH32_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBE0139644.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="KLH32_002893"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="KLH32_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBE0139645.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="KLH32_002894"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="KLH32_002894"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="KLH32_002895"
     CDS             19137..20378
                     /locus_tag="KLH32_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE0139646.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5358 c         4815 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcgaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgagccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAEEDS010000073        20289 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEEDS010000073 DAEEDS010000000
VERSION     DAEEDS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20289
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.073"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="KLH32_002896"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="KLH32_002896"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBE0139647.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="KLH32_002897"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="KLH32_002897"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBE0139648.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="KLH32_002898"
     CDS             complement(1728..2999)
                     /locus_tag="KLH32_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139649.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="KLH32_002899"
     CDS             complement(3182..4387)
                     /locus_tag="KLH32_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139650.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLHSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="KLH32_002900"
     CDS             complement(4487..4843)
                     /locus_tag="KLH32_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBE0139651.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="KLH32_002901"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KLH32_002901"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="KLH32_002902"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="KLH32_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBE0139652.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="KLH32_002903"
     CDS             6773..9829
                     /locus_tag="KLH32_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE0139653.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="KLH32_002904"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="KLH32_002904"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBE0139654.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="KLH32_002905"
     CDS             10336..10524
                     /locus_tag="KLH32_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBE0139655.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="KLH32_002906"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="KLH32_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBE0139656.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="KLH32_002907"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="KLH32_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBE0139657.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="KLH32_002908"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="KLH32_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBE0139658.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="KLH32_002909"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="KLH32_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBE0139659.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="KLH32_002910"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="KLH32_002910"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBE0139660.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLIAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="KLH32_002911"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="KLH32_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBE0139661.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="KLH32_002912"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="KLH32_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBE0139662.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRIAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="KLH32_002913"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="KLH32_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBE0139663.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="KLH32_002914"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="KLH32_002914"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBE0139664.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5317 a         4855 c         5175 g         4942 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacaccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa actgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgt
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gtgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa ggtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gactgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagcctg gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga ttccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca taacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat agcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcat
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    20281 atgctgata
//
LOCUS       DAEEDS010000074        20217 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEEDS010000074 DAEEDS010000000
VERSION     DAEEDS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.074"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="KLH32_002915"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="KLH32_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="KLH32_002916"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="KLH32_002916"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBE0139665.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="KLH32_002917"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="KLH32_002917"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBE0139666.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="KLH32_002918"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="KLH32_002918"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBE0139667.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KLH32_002919"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KLH32_002919"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBE0139668.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KLH32_002920"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KLH32_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBE0139669.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KLH32_002921"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KLH32_002921"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBE0139670.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KLH32_002922"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KLH32_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBE0139671.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KLH32_002923"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KLH32_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBE0139672.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KLH32_002924"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KLH32_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBE0139673.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KLH32_002925"
     CDS             complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KLH32_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBE0139674.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KLH32_002926"
     CDS             complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KLH32_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBE0139675.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KLH32_002927"
     CDS             complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KLH32_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBE0139676.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12654..13094)
                     /locus_tag="KLH32_002928"
     CDS             complement(12654..13094)
                     /locus_tag="KLH32_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBE0139677.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KLH32_002929"
     CDS             complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KLH32_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBE0139678.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KLH32_002930"
     CDS             complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KLH32_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBE0139679.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14036..15076
                     /locus_tag="KLH32_002931"
     CDS             14036..15076
                     /locus_tag="KLH32_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0139680.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15112..16113)
                     /gene="add"
                     /locus_tag="KLH32_002932"
     CDS             complement(15112..16113)
                     /gene="add"
                     /locus_tag="KLH32_002932"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBE0139681.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KLH32_002933"
     CDS             complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KLH32_002933"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBE0139682.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KLH32_002934"
     CDS             complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KLH32_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBE0139683.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19166..20194
                     /gene="malI"
                     /locus_tag="KLH32_002935"
     CDS             19166..20194
                     /gene="malI"
                     /locus_tag="KLH32_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBE0139684.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5140 a         5120 c         5199 g         4758 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg
     9361 cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg gcctgttgct
     9421 gttccagctt gcgtgctttg gcgcgggcaa tagccgcttc gacggcagct ttgcgcggat
     9481 caacctgttc ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggcgc
     9541 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
     9601 tagcctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     9661 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
     9721 gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca gcaacggttg
     9781 ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
     9841 cccgttctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
     9901 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
     9961 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc
    10021 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    10081 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aagggaatat
    10141 tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg ttatgcgtgg
    10201 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatcca
    10261 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    10321 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    10381 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    10441 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    10501 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    10561 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    10621 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    10681 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    10741 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    10801 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    10861 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca gcgttgataa
    10921 tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg aatccggcac
    10981 cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    11041 agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    11101 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    11161 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    11221 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    11281 gtttcagtgg aataacaaaa tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg
    11341 tctgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    11401 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    11461 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    11521 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    11581 atgacacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    11641 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    11701 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    11761 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    11821 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    11881 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    11941 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    12001 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    12061 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    12121 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    12181 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    12241 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    12301 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    12361 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    12421 acgtcagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    12481 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    12541 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    12601 cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga
    12661 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    12721 gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg
    12781 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    12841 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    12901 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    12961 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    13021 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    13081 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    13141 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    13201 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    13261 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    13321 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    13381 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    13441 gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct
    13501 gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc
    13561 tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg
    13621 aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt
    13681 accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga
    13741 atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc
    13801 cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc
    13861 tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc
    13921 tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga
    13981 gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag
    14041 cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc
    14101 cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa
    14161 agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga
    14221 tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa
    14281 agcggcgctt gaggcgggta aatatgtggt cgttgataaa ccctttaccg tgacactgtc
    14341 acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca
    14401 taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct
    14461 gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg
    14521 ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct
    14581 tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt
    14641 acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg
    14701 agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg
    14761 atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg
    14821 cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt
    14881 ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta
    14941 ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca
    15001 ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac
    15061 gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt gttacttcgc
    15121 ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca tttccagacc
    15181 attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg cggcaacggt
    15241 atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgctggcacg
    15301 aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt
    15361 ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat
    15421 cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg
    15481 aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca
    15541 gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga atccaagttc
    15601 atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc
    15661 ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac
    15721 accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac
    15781 aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagcgcag
    15841 gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga
    15901 ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt
    15961 ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa gcgagatatt
    16021 atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg
    16081 gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa
    16141 gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta
    16201 cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca gccacacctt
    16261 tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac
    16321 cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct ttttgcaacg
    16381 cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg gattgcggga
    16441 tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac gtcaggttat
    16501 ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag gcggcgatat
    16561 gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc agtgccgata
    16621 aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc agcgcgggaa
    16681 tattgaaact tttcgatccc gatgtcagca acgcccagtc tccccgcgcc acattactcc
    16741 agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg gaaataaccc
    16801 gcacatcatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt
    16861 tcccggtagg attctgtggg ttacacagga gcataatttt acattctggt ttcgccaaaa
    16921 cggcttccag cttgcccata tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg
    16981 gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggcgtgt
    17041 ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga
    17101 tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc tgggtggaaa
    17161 accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc
    17221 cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca aaatccatgt
    17281 ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact
    17341 gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt
    17401 atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc
    17461 cgataacaac ctgcaggtta tgttgattaa gttgtactac gccaattgcc cgattgtcct
    17521 tcagtgcctg cacattaaca agcgacatat cttccacaga caaacgcaga cgggtaatgc
    17581 agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt
    17641 tgtaacctga tttacccggc gcaccggcaa cggctttttc gattgagctg gcaacttcgc
    17701 tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag atgacgtagt
    17761 aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt gacagaccat
    17821 gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc
    17881 cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa
    17941 ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca acgacgcagg
    18001 cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg cgcgcgcagt
    18061 gatacatagc taacgctgca cctggtagac cgccgagaaa cgcaggcatt ttaccttgcg
    18121 aaaggaaacg cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct
    18181 ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ctcctgcgtg ccgcctgcgt
    18241 cggtaaagcg aattaatgcc accagaatgt gatgcagacc aaacggcaac agcagacgtt
    18301 caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata tggcccaagc
    18361 cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg acaaggccca
    18421 tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg
    18481 gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg gcgatcaccg
    18541 caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg ttattggctt
    18601 tcagaacggc ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag aagtttaccg
    18661 cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct ttattttcgc
    18721 gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta aaagcaaacg
    18781 agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc aggaccggga
    18841 tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata atgccgcaga
    18901 acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag aactcccaca
    18961 gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact cctcgtagag aacaatctgg
    19021 attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag tcataaatca
    19081 cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt ttatctgtca
    19141 cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca ttcatgatgt
    19201 tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttaatg gcaaagggcg
    19261 aatctctacc gccacaggag aacgcgttaa cgccgccatt gaagagctgg gatttgtgcg
    19321 caatcgacag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt tgatcgtccg
    19381 tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag ctctggaagc
    19441 acagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc tggcacagcg
    19501 gttttcactg ttactgaatc agggtgtcga tggtgtggta attgccgggg ctgcaggaag
    19561 cagcgatgac ctgcggcgga tggcagaaga aaaagctatc ccggtgattt tcgcttcccg
    19621 tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg ctgcacagtt
    19681 gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg gagggcaaag
    19741 ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac taaaatttgg
    19801 cctgccgttt cacagcgatt gggtgttgga gtgcacttcc agccagaagc aagccgcgga
    19861 agctatcacg gcgcttttac gtcataaccc gaccatcagt gccgtggttt gctataacga
    19921 aactattgcg atgggggcat ggtttggttt gctgaaagca gggcggcaaa gcggggaaag
    19981 cggagtcgat cgttactttg agcaacaggt ttcgctggcg gcatttaccg atgcgacacc
    20041 aaccacactt gatgatatac ctgttacctg ggccagcacg ccagcgcggg aacttggtat
    20101 cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt cacgcaatct
    20161 tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc tatactt
//
LOCUS       DAEEDS010000075        19936 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEEDS010000075 DAEEDS010000000
VERSION     DAEEDS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19936
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.075"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..307
                     /locus_tag="KLH32_002936"
     CDS             <1..307
                     /locus_tag="KLH32_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBE0139685.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            279..2207
                     /locus_tag="KLH32_002937"
     CDS             279..2207
                     /locus_tag="KLH32_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBE0139686.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2191..2394
                     /locus_tag="KLH32_002938"
     CDS             2191..2394
                     /locus_tag="KLH32_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0139687.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2391..3984
                     /locus_tag="KLH32_002939"
                     /pseudo
     CDS             2391..3984
                     /locus_tag="KLH32_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3974..5479
                     /locus_tag="KLH32_002940"
     CDS             3974..5479
                     /locus_tag="KLH32_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBE0139688.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5516..5863
                     /locus_tag="KLH32_002941"
     CDS             5516..5863
                     /locus_tag="KLH32_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBE0139689.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5921..6949
                     /locus_tag="KLH32_002942"
     CDS             5921..6949
                     /locus_tag="KLH32_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBE0139690.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7001..7387
                     /locus_tag="KLH32_002943"
     CDS             7001..7387
                     /locus_tag="KLH32_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBE0139691.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7399..7776
                     /locus_tag="KLH32_002944"
     CDS             7399..7776
                     /locus_tag="KLH32_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0139692.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7763..8353
                     /locus_tag="KLH32_002945"
     CDS             7763..8353
                     /locus_tag="KLH32_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0139693.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPMSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8350..8751
                     /locus_tag="KLH32_002946"
     CDS             8350..8751
                     /locus_tag="KLH32_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0139694.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8762..9502
                     /locus_tag="KLH32_002947"
     CDS             8762..9502
                     /locus_tag="KLH32_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBE0139695.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9561..9947
                     /locus_tag="KLH32_002948"
     CDS             9561..9947
                     /locus_tag="KLH32_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBE0139696.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9956..10285
                     /locus_tag="KLH32_002949"
     CDS             9956..10285
                     /locus_tag="KLH32_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBE0139697.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10257..12995
                     /locus_tag="KLH32_002950"
     CDS             10257..12995
                     /locus_tag="KLH32_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE0139698.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12995..13324
                     /locus_tag="KLH32_002951"
     CDS             12995..13324
                     /locus_tag="KLH32_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0139699.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13324..14022
                     /locus_tag="KLH32_002952"
     CDS             13324..14022
                     /locus_tag="KLH32_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBE0139700.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14027..14770
                     /locus_tag="KLH32_002953"
     CDS             14027..14770
                     /locus_tag="KLH32_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0139701.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14707..15309
                     /locus_tag="KLH32_002954"
     CDS             14707..15309
                     /locus_tag="KLH32_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBE0139702.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15370..18850
                     /locus_tag="KLH32_002955"
                     /pseudo
     CDS             15370..18850
                     /locus_tag="KLH32_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            18918..19517
                     /locus_tag="KLH32_002956"
     CDS             18918..19517
                     /locus_tag="KLH32_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBE0139703.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19539..>19936
                     /locus_tag="KLH32_002957"
     CDS             19539..>19936
                     /locus_tag="KLH32_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE0139704.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         4647 a         4694 c         6299 g         4296 t
ORIGIN      
        1 actactcatt gccagatggg aactgaccgc agaacaggct gtcgcacagc agttaaaaaa
       61 tcaggtgtca aaaggaaatc ttatcgatac cggattttgt atttttgccc ttagtaagct
      121 ggcgatggct ttatccagta cgcttgactc cattcctttg tccatgcaac gacagttccc
      181 ggatttaacg ccgcgtcata ttgatcatct gaaaatcctt attgctaagg gagcgaatca
      241 gtgcgcgcgt gcgggggata aattaccgga tttactcgat gaatatatca gaacaacaac
      301 tgagtaatat gatggtggca gtcactatcg cactgcagcc actggtcagg gtattgccgg
      361 tgacggctgt agaatgggcc gatcaaaatt attacctgcc taaagaatcg tcatacggtg
      421 agggggaatg gaaaacgctg ccgtttcagg tcgccatcat gaactgcatg ggcaatgacg
      481 agattcgtac agttaacctg attaaatctg cccgtgttgg ttatacaaaa atggtgcttg
      541 gtgtgatcgg gtattttatt gagcacaaat cccgcaacac tcttcttttt cagccgacgg
      601 actcagctgc tgaagatttt atgaagtctc acgtggaagc aacaattcgt gatgttcctt
      661 gcctgaaaaa actttctccc tggctgggcc ggaaacaccg ggataatacg ctcacactga
      721 agcgtttttc ctctggtgtg gggttctggt gtttgggtgg tgctgctgcc aaaaactacc
      781 gtgaaaaatc tgtcgatgtg gtctgctatg acgaactctc ctcgtttgaa ccggatgtgg
      841 aaaaagaagg ttcgccaaca ctgcttggcg ataaacgtat tgaaggttcg gtatggccaa
      901 aatccatacg cggttcaacg ccaaaaatta aaggctcctg tcagattgaa aaagctgcga
      961 atgaatctgc gcatttcatg cggttttatg tcccttgccc tcattgcggg gaggagcagt
     1021 atctgaagtt tggcgatgat gcgacgtcgt ttggcctgaa atgggagaag ggtaaaccgg
     1081 aaacggtgta ttacctgtgt gaacataatg gctgcgtgat ccgtcagtct gaacttgatc
     1141 agaccggggg gcgctggatt tgtgacaata ccgggatgtg gacgcgtgac ggtctgacgt
     1201 ttttcagtgc ttcgggtaat gaaattccgc cgccgcgctc catcactttc catatatgga
     1261 cggcgtacag tccgttcacc acctgggtac agatagtctg tgactggctg gatgcactga
     1321 aagatcccaa cggcgtgaaa acttttgtga acaccacgct gggcgagacc tgggaagagg
     1381 ccgtgggcga aaaactcgat caccaggtac tgatggataa ggttgtgcct tacacggcaa
     1441 cggtgcctgt ccgggtggtt tatctgacgg cgggcattga ctcgcagcga aaccgttttg
     1501 agatgtatgt ctgggggtgg gctccgggag aggaagcctt tctggtggat aaaatcatca
     1561 tcatgggacg tcctgatgag gaagagacgc tgttacgtgt ggatgcggcg atcaataaaa
     1621 aataccgcca tgcagacggt accgaaatga ccatttcccg tgtctgctgg gatatcgggg
     1681 ggatcgatgg cgaaatcgtg tatcagaggt caaaaaaaca cggtgttttc cgggtactgc
     1741 cggtaaaagg tgcctctgtc tatggcaagc cggtgatcac catgccgaaa acccgcaatc
     1801 agcggggtgt gtatctgtgt gaggtgggga cggataccgc aaaagaaatt ctctatgccc
     1861 gaatgaaggc cgatcccacc cctgtgaatg aggccacatc gtatgccatc cgctttcctg
     1921 ataatccgga gattttttcg cagacagagg cacaacaact ggtggcggag gaactggtgg
     1981 aaaagtggga aaaaggaaag atgcgtctgt tgtgggataa caaaaagcgg cgtaacgaag
     2041 cgctggactg cctggtgtat gcctatgcgg cattacgtgt gtccgtgcaa cgctggcaac
     2101 ttgatctggc tgtgctggcg gcatcccgca aggatgcaac tgcacaaccg acccttgaag
     2161 aactggcagc gaagctgtcc ggaggagtga atggttacag tcgctgaact gcaggcgctg
     2221 cgtcaggcgc gccttgattt attaaccggt aaacgggttg tgtcggtaca gaaagacggt
     2281 cgcaggatcg aatatacggc agcgtccctg aatgagctta accgtgcgat caacgatgcg
     2341 gagtcggttc tgggtacgac ccgacgccgc cgaccgctgg gagtgaggtt atgaaacgaa
     2401 cgcctgtcct gattgatgtg aacggcgttc cgcttcgtga gagtctcagc tacaacggag
     2461 gcggcgcagg atttggcggg caaatggcag agtggttacc accggcgcag agtgccgatg
     2521 cggccctgtt gcctgcattg cgtctgggga atgcccgggc agatgatctg gtgcgcaata
     2581 acggtatcgc ggctaatgcg gtggccctgc ataaggatca tatcgtcggg cacatgtttc
     2641 ttatcagcta ccgtctgaac tggcgttggc tggggatgcg ggagaccacg gcaaaaagtt
     2701 ttgtcgggga ggtggaagca gcctggtcgg aatacgcaga aggaatgttt ggcgagatcg
     2761 acgtggaagg gaaacgcacg tttacggaat ttatccgtga aggggtgggc gtgcatgcct
     2821 ttaacggcga aatctttgtg cagccggtct gggatacgga aaccacgcag ttattccgta
     2881 cgcgttttaa agccgtgagt ccgaaacggg tggacacgcc aggacacggt atggggaacc
     2941 actttctgcg ggccggggtt gaggtcgatc ggtatgggcg ggcgattgcg taccatatct
     3001 gtgaggatga ttttccgttc tctggtgccg gacgctggga gcggatcccg cgtgagcttc
     3061 ccaccgggcg tccggccatg ctgcatattt ttgagccggt ggaggacggg cagacccgtg
     3121 gggccaatca gttttacagc gtaatggagc ggatgaagat gctggactcc ctgcaggcca
     3181 cgcagcttca gtcggccatt gtgaaggcga tgtatgcagc cacaatcgaa agtgaacttg
     3241 ataccgaaaa ggcatttgaa tatatcgcgg gggcaccgca aggtcagcag gataatccgc
     3301 ttattaatat tctggagaag ttcacccgct ggtatgacac gaataacgtg acgctgggcg
     3361 gtgttaaaat tccgcacctt ttttcctggc gatgcgctga acctgcagac agcgcaggat
     3421 tcagacaatg gattttcggc gcttgaacag gcgctgctgc ggtatattgc agccggtctt
     3481 ggcgtttcct acgaacagtt gtcccgtgat tactcgaagg tcagttactc aagtgcccgt
     3541 gcctccgcca atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg
     3601 ctggccacgc agatgttttc ctgctggctg gaagaggcgc ttcttcgggg gattattcgt
     3661 ccgccacggg cgcgttttga tttttaccag gcgcgttcag cctggtcacg ggcagagtgg
     3721 attggctccg gaagaatggc cattgacggg ctcaaggagg tccaggaatc ggtgatgcgc
     3781 attgaggccg gactgagcac gtatgagaaa gaactggcac tgatgggcga agattatcag
     3841 gacattttcc gccagcaggt cagggaatcc gcagagcgcg aaaaagccgg actctcacgt
     3901 ccggtgtgga tagcccaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag
     3961 gagacaacat cacgtgagac gtaatctttc acatattatc gctgcggcat tcaatgagcc
     4021 gctgcttctg gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gtgagatggg
     4081 cgcagcaagc ctttcggtac cgcaacagca ggtacagctt gacgctcccg ggatgctggc
     4141 tgaaacggac gagtacatgg ccggaggcaa acgacctgcc cgtgtttacc gggtggtgaa
     4201 cggtattgct gtactgccgg tgaccggcac gctggtacac cggctgggtg gcatgcggcc
     4261 attttccgga atgactggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga
     4321 cactcaggta cggggcgtac tgctggacat tgacagtcct ggcgggcagg ccaccggcgc
     4381 gtttgactgc gctgacatga tttaccgtct ccggcagctg aagcctgtct gggcactgtg
     4441 caatgacacg gcctgttcag ccgccatgtt actggcatcg gcctgctccc gacggctggt
     4501 tacccagaca tcccgtatcg gctccattgg ggtgatgatg ggccatgtca gctatgccgg
     4561 tcatctggca caggccggtg tggatatcac gctgatttac gccggatcgc acaaggtgga
     4621 tggcaatcag tttgaatccc tgccagcaaa ggttcgccag gacatgcagc agcgcattga
     4681 tgcggcgcgc cggatgtttg ctgaaaaagt ggcgatgtat accggtttat ctgtggatgc
     4741 ggttatggga acagaggccg ccgtttttga aggtcagtcc ggtattgagg ccgaactggc
     4801 ggatgaatta atcaatgcgt cggacgccat cagcgtgatg gctgcgacgc tgaacacaca
     4861 cgatacagga ggcactatgc cgcaattaac tgcaacggag gctgccgcgc aggagaacca
     4921 gcgagtgatg gggatcctga cgtgtcagga agcgaaagga cgtgaacaac tggccacgat
     4981 gctggcaggg caacagggta tgagcgttga gcaggcccgg gcgattctgg ccgtggcggc
     5041 accgcaacaa tctggtgtat ctgcgcagag tgaagccgat cgcattatgg catgtgaaga
     5101 ggcgaaagga cgtgaacaac tggcggcaac gctggcggcg atgccggaga tgacggtgga
     5161 aaaagcccgt ccgattctgg ctgcttcacc gcaggcgaat gccggaccct cacttcgtga
     5221 tcagattatg gccctggatg aggcaaaagg ggcagaggcg caggctgaaa aactggcggc
     5281 ctgtccgggg atgaccgtgg agaatgcccg ggctgtactg gcggcagcct caggcagggc
     5341 agaaccggtt tctgcatcca caaccgccat gtttgaacat ttcatggcga accattcacc
     5401 ggcagcggta cagggtggcg tgccacagac gtcagcagac ggtgatgcgg acgtgaaaat
     5461 gctcatggcc atgccatgaa gtcagtgctg accatcaata tgaggttttt acaatatggt
     5521 aacgaaaacc atcactgaac agcgtgcgga agtacgtatt tttgccggta atgatccggc
     5581 ccataccgcc acaggcagca gcgggatttc ctcggcaaca ccggctctga cgcccctgat
     5641 gctggatgag gataccggga aactggtggt ctgggacgga cagaaggcag gccatgtggt
     5701 tggcatactg gtactgccgc ttgaaggtac agagacggtg ctgacgtatt acaagtcggg
     5761 gacctttgcg acggaggcca tccactggcc tgagagagtg gatacacaca aaaaggcgaa
     5821 tgcctttgcc ggcagtgccc tgagtcacgc agcgctgccg taacatttta tcaggtcact
     5881 gcggtagcct gactgatttc tgaatgaaag gaactgattt atgggattgt ttacgacccg
     5941 ccagttactc ggttataccg aacaaaaagt taaatttcgt gcgctgtttc tggagctgtt
     6001 tttccgccgt acggtgaatt tccataccga agaggtaatg ctggacaaaa ttaccggaaa
     6061 aacgccggtg gcggcctatg tctccccggt tgttgaagga aaggtgttgc gtcaccgtgg
     6121 cggtgaaact cgtgtactgc gcccgggcta cgttaagccg aaacacgaat ttaattacca
     6181 gcaggcggtg gagcgtcttc ccggtgaaga tccggcccag ctgaacgacc cggcttaccg
     6241 tcgcctgcgt atcatcaccg ataacctcaa acaggaagag cacgccattg tccaggtgga
     6301 agagatgcag gcggtgaatg ccgtgctgta tggcaaatac accatggaag gagagcagtt
     6361 tgaaactgtt gaggtcgatt ttggccggtc ggtgggaaat aacattacgc aggcaggagg
     6421 gacggagtgg agcgctcagg accgcgatac ctttgatccg acgcatgatc tggatgcgta
     6481 ctgtgatttt gcttccggca ccatcaatat tgccattatg gatggtaagg tctggcgtct
     6541 gctgaatggc tttaagctgt tccgcgaaaa actggatacc cgtcgtggct caaattcaca
     6601 gctggaaacg gcagtgaaag acctgggggc ggtggtgtcc ttcaaagggt attatggtga
     6661 tctggcaatc gtggtggcaa aaacgtccta tgtggcagag gacggtaccg aaaaacgtta
     6721 tctgccggac ggcacactgg tactgggaaa tacggccgca gagggtattc gttgctacgg
     6781 tgccattcag gatgcgcagg cattgtctga aggcgtggtg gcgtcttccc gttatccgaa
     6841 acactggctg accgtgggtg atccggcgcg tgaattcacc atgacgcagt ccgcgccgct
     6901 gatggtgctg ccggatccgg atgagtttgt ggtggtgcag gtgaaataat ccgtgagcgg
     6961 gagctaaatg ctcccgtttt tcattcaatc aggagcgggt atggctacga aagaagaaaa
     7021 cctgaaacgt ctttgtgagc tggctgagag gctggggcgt gaaccggatg tctccggtag
     7081 tgcggcagat attgcgcagc gagtggcgga actggaggaa gagcttggag atgcggggga
     7141 ggtcgcagag ccggatacgt actcaccatc gaaagatgat ccgaccgcgc ataaaggcga
     7201 gtcagttcct gaacagccag gcattgttac cggagacgaa acggggcagg ttacggtggt
     7261 ggcactggcg acactgcata caaaatgtaa tggttcagtg atatttgttt caccgggaac
     7321 gtcgtttcgt gtttctgctg aggtggctgc cagcatggcg gcaggtggtc tggcaaaaag
     7381 gcagtaacag gagatgtcgt ggacggtttc gataatctgt ttgatgctgc gcttgccggt
     7441 gtcaatgaag tcattctccg tgatatgggg atcagcgccg tcattacgtc cggagaactt
     7501 gaggggacac acctgacagg ggtttttgat gatcccgaaa gtatttcttt tgttgccggg
     7561 gggatccggc ttgaggattc ctcaccgtgc ctgtttgtga aaacggcaga tatatcgcag
     7621 ttacgccgtc aggatacgtt aactatcggg gatgattctt tctttgtgga tcgtattact
     7681 ccggatgacg ggggatgctg ttatatccgg ttaaggcggg gcagtcctgc cccacagaat
     7741 aatgccagaa tgaggcgata tgatgaaggg acttgagaat gccatccgca atctgaacag
     7801 ccttgatacc cgtatggtgc cacaggccag cgcatgggcg ataaaccgtg tggcacagaa
     7861 agcggtctcg gttgccaccc ggcaggttgc cgggaatacc gttgcgggcg ataaccaggt
     7921 gaaagggatc cccctgaaac tggtacgtca gcgtgtccgg gtgtttaaag ccagtccgtc
     7981 aggaaaaatg acggccagga tccgcgttaa ccggggcaat ctgcccgcca tcaaactgaa
     8041 cacaacacgg cggcgtgctg gtgaaggact gagagtggga aaatactttt tccggggggc
     8101 atttgttcag caactggcga atggccgctg gcatgttctg aggtgtcttc ctgaagcgcg
     8161 ttttgcaaca gggcatgacc atcagggcag gctaagaaaa aatcgtcttc ctgtggaggt
     8221 ggtgaaaatc ccgatgtccg gaccgctgac acaggcattt gaagatgccc gcgaccgcat
     8281 cattgctgcg gaaatgccga aacagctggg gtatgccctg aaacaacaac tgaggttaca
     8341 tctgagtaaa tgaaccgaca cacacaaatc cgtcagagcg tgctggcacg ccttcgggaa
     8401 cagtgtggag acagcgccac gttttttgac gggcttccgg catttattga tgcgcaggaa
     8461 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgaa
     8521 gatgactggc aggctgttct gcatattgcc gtcttcatcc gggcacaggc accggattca
     8581 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     8641 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     8701 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     8761 aatgaccaca ccaaatccac tggcaaagac gaaaggtgcg gggacgacgt tctggatgta
     8821 taccggcaac ggcgatgcgt ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     8881 gatggtgaag gatctgcaac ctggcgaaat gaccgctgat gcagaagatg acacttatct
     8941 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg ttggtgatac
     9001 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     9061 cgactccggt gaagtctgcg cgtttcgtat caaatatccc aacggcactg ttgatgtttt
     9121 ccgcggctgg ctgagctcac tgggtaaaac cattgcctca aaagacgtga tgacccgcac
     9181 tgtgaaaatc agcggtgtgg ggcgtccgta tctggcagag gaaggcactg aaaccgtggg
     9241 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     9301 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     9361 agatcctcag actgcttcgg tgaccctgag cgggcttgtg gccacggtga aaggcgtgaa
     9421 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     9481 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     9541 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     9601 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     9661 agcgaacggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     9721 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     9781 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     9841 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     9901 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     9961 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcgga tgctgtcgga
    10021 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
    10081 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
    10141 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
    10201 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
    10261 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
    10321 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
    10381 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
    10441 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
    10501 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
    10561 aaatgcgcga ggaacaggcg caggccaggg cggctcagct gggggtaagc agtgctgcag
    10621 aagtgtatat ccgcaaaatg gagcgggcag gaaaagccac acattcgctg gggctgaaaa
    10681 gtgcggcagc ccggcgggag ctgggggtgt taatcagtca gatggcgcgc ggcaattttg
    10741 gtgcgctgag gggatccggg ataacgctgg ctaaccgtgc cggatggaca ggcgcactga
    10801 tgtcgccgaa aggcatgatg actggcggcg ttattggcgg acttgtcgcg gcggtcctgg
    10861 gtctgggtaa agcctggcat gacggccgga aggagggcga gaaatttaac cgtcagctgg
    10921 cgctgacggg acattatgcc ggtgtcactg tcgggcagtt gtggaaactc agccgggcca
    10981 tatccgggaa tggtatcacg caacatgcgg cagccggtgc gctggcacag gtggtgggca
    11041 gtggggcatt tcatggaaac gatattggta tggtggcgaa agctgccgca cagatggagc
    11101 gatcggttgg ccagtcggtc agcgatacca taaatcagtt taagcggctg aaggatgatc
    11161 ctgtaaatgc cgcgaaggcg ctggacaatg cgctgcattt tttgaatgcc actcagcttg
    11221 agcagatacg cgtccttggg gagcaggggc ggtccagtga tgcggcccgg atcgccatgt
    11281 ctgcgctggc agaggaaacc ggtaaacgca catctgatat tgataataat ctcaatgcgc
    11341 tgggtagtac gctgcaaacc ttgtctgact ggtggaagca gttctgggat gcggccatga
    11401 atattggtcg tgaagactcg ctggatgcgc agattgatgc gttacaggaa aaaattcagc
    11461 gcgcgaaaaa atatccgtgg acaaacgcct ccacacaggt ggagtacgat cagcagcgtc
    11521 ttaacgatct tcaggagaaa aaacgccgga aggatttgca ggatgcaaaa gcgcaggcag
    11581 aacggaatta ccaggagcaa cagaaacgcc ggaatgctga aaatgccgcg ctgaaccgga
    11641 tgaatgaaac ggaagctgca cgacatcagc gggaaattgc gcgtattaat gccatgcagt
    11701 acgccgacca ggctgtcagg gatgcggcga tacagcgtga aaatgaacgt tacgaaaaag
    11761 ccattaagaa aaatacacgg gcaacccgta atgatgaggc cacccggtta ttgctgcagt
    11821 acagtcagca gcaggcacag gtggaagggc agattgctgc tgccagacag tcagcaggca
    11881 ttgccactga aaggatgact gaagcgcata aacagcttct ggccctgcag cagcgcatca
    11941 gcgacctgga cgggaaaaag ctgacggcag atgaaaagag tgtgctggcc cgtaagaatg
    12001 agctgattca ggcgctgacg ctgctggatg tgaaacagca ggagctgcag aaacagacag
    12061 cgcttaacga cctgagaaaa aaaacggttc agctgaccag ccagctggca gacaaagaac
    12121 gtgcactgcg tgagcagcac aatctggata ttgccactgc aggtatgggg gataagcagc
    12181 ggcagcgcta ccaggcacag ttgcgcatcc ggcaggaata ccggcaacag ttgcaacagc
    12241 ttgagaatga cagtcgccag aaaggcactt acgggacgga ggactaccgg agggctgagg
    12301 aggtgctgaa ggggagcctg aagcgacaac tgaatgaaaa caaacgctac tggcaggaac
    12361 tggaagtggc gcagggcgac tggaaaaacg gtgccatgcg ggcgtttcag aattttacgg
    12421 cggatgcgga taatgcggcg ggaacggcag aacagatgtt tacagtggca ttcagcagtg
    12481 ccggtaatgc actggcgaca ttctgtacca ccggtaagct gaattttaaa tccttcacct
    12541 cttccctgtt gtcagatatg gccagaatta tggcacagat ggccatgatg caggcggtaa
    12601 agggcgtcgg ttctttattc ggcttcacga ctaatgctga tggcggtgtt taccagtctg
    12661 ctgatttgag tcgctacagt ggcacggtgg ttaaccgtcc gacgtttttt gcttttgcaa
    12721 aaggcgcggg tgtgatgggg gaagcgggac ctgaagccat tctgccactg cgtcgcggtg
    12781 ctgatggtaa gctgggggtt gtggcggata ctggtggttc aggcatggtg atgtttgccc
    12841 cgcagtacaa cattgagatc aataacgacg gcacgaacgg gcagataggt ccggctgccc
    12901 tgaaggtggt ttatgacctc gggaaaaaag cggccgcgga ctttatgcaa cagcaggccc
    12961 gtgatggtgg ccggttaagt ggagcatacc ggtaatggag acgtttcact ggaaagtgcg
    13021 cccggatatg aatgtggtat cagagccgaa agtggtgaca gtgaagctgg gcgatggtta
    13081 tgaacagcgt cgtgcggcgg gactgaataa ccagttgtcg acttacagcg tgacgatacg
    13141 tgttcgtaaa ggtgaacacc catctttaaa agcctttctg gaacggcacg gtggcgtccg
    13201 cgcatttcag tggacgccac cttatgactg gaaactgatc agggtggttt gtcgtaaatg
    13261 gtcggcaagc gtgggggcgt tatgggtgac tgtaacggcc gattttgaac aggtggtgaa
    13321 ctgatgcagg atatccggca ggaaacactg aatgaatgca cccgtgcgga gcagtcagcc
    13381 cgggtggagc tctgggaaat cgatctgaca gaggtcggtg gtgagcgtta ttttttctgt
    13441 aatgagcaga acgaaaaagg tgagccggtc acctggcagg ggcggcagta tcaggcatac
    13501 cccattcagg ggtcggggtt tgaactgaac ggcaggggct gtgctgcccg tccgacactg
    13561 acggtctcta acctgcacgg catggtcacc gggatggcgg aagatctgca gagtctggtc
    13621 ggcggaacgg tggtccggcg taaggtttac gcccgttttc tggatgcggt gaacttcgtc
    13681 aacggaaaca gcgacgccga tccggaacag gaggtgatca gccgctggcg catcgagcag
    13741 tgcagcgaac tgagcgcggt cagtgcctct tttgtgctgg ccacaccaac ggagacggat
    13801 ggcgcggttt tcccggggcg tatcatgctg gcgaatacct gtatgtggac ttaccgttct
    13861 gatgagtgtg gttacacggg cagggcagtg gctgacgagt tcgacaaacc aacaacggat
    13921 atccggaagg acaaatgcag caagtgtatg cgcgggtgtg agttgcgcaa caataccggt
    13981 aatttcggcg gtttcctttc catcaataaa ctttctcagt aaatccatga cacagacaga
    14041 atcagcgatt ctggcgcacg cccggcgatg tgcgccagcg gagtcgtgtg gcttcgtggt
    14101 gagaacgccg gagggagaca ggtatcttcc cagcgagaat atctccggtg agccggagga
    14161 acggttccgg atggctccgg aggactggct gcgggcacaa atgcagggtg agattgtggc
    14221 actggtccac agtcatcccg gtggtctgcc ctggctgagt gaggctgacc ggcggctgca
    14281 ggtgcagagt gatttgccgt ggtggctggt ctgccggggg gcgattcaca agttccgctg
    14341 tgtgccgcat ctcaccgggc ggcgctttga gcacggggtg acggactgtt acacgctgtt
    14401 ccgggatgct tatcatctgg cggggattga gatgccggat tttcatcgcg gggatgactg
    14461 gtggcgtcac ggtcagaatc tctatctgga taatctggag gccacagggc tgtatcaggt
    14521 gccgttgtca tcagcacaac cgggcgatgt gctgctgtgc tgttttggtt catcggtgcc
    14581 gaatcatgcc gccatttact gcggcgatgg tgagctgctg caccatattc ctgaacaact
    14641 gagtaaacga gagaggtata ccgacaaatg gcagcgacgc acacactccc tctggcgtca
    14701 ccgggaatgg cacgcatctg cctttacggg gatttgcaac gatttggccg ccgcatcgac
    14761 cttcgtgtga aaacgggggc tgaagccatc cgcgcactgg ccacacagct cccggcgttt
    14821 cgtcagaaac tgagtgacgg ctggtatcag gtgcgcattg ccgggcgtga tgcaggtgaa
    14881 accgaattgt ctgcccgtct taatgagccg ctggcaaatg gtgcagtgat ccacatagta
    14941 ccgcgtctgg tgggagctaa aagtggcggt gtgtttcagg cggtgctggg ggcagctgtt
    15001 atggcggttg ctatatggat gccgggggta ggaattatgg cgagtaatct gctgttttct
    15061 ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc tggcaccgaa agccagaact
    15121 ccccgtacac agacaacgga taacggcaaa cagaacacct gtttctcctc actggataac
    15181 atggttgccc agggcaatgt tctgcctgtt ctgtacggtg aaatgcgcgt ggggtcgcgg
    15241 gtggtatctc aggagatcag cacggcagac gaaggggatg gtggtcaggt tgtggtgatt
    15301 ggtcgctgat gcaaaatgtt tcatgtgaaa ccgcctgcgg gcggttttgt cgtttatgga
    15361 gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc gaaggacaac
    15421 ctgaaatcca cgcagttgct gagtgtgatt gatgccatca gcgaagggcc ggttgacggt
    15481 ccggtggatg gattaaaaag cgtgctgctg aacggtacgc cggtcctgga tagcgagggg
    15541 aagacaaact tttccggtgt tacggtggtg ttccgcgccg gcgagcagga gcagacaccg
    15601 ccggaggggt ttgaatcttc cggctcagag actgtgctgg gtacggaagt gaaatacgac
    15661 acgccgatca cccggaccat cacgtcggca aacattgatc gtctgcgcct gaccttcggt
    15721 gtgcaggcac tggtggaaac cacctcaaag ggggaccgga atccgtcgga agtccgcctg
    15781 ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa aagacatcac cattaagggc
    15841 aaaaccactt cgcagtatct ggcctcggtg gtggtgggta acctgccgcc gcgcccgttt
    15901 aatatccgga tgcgcaggat gacaccggac agcaccacag accagctgca gaacaaaacg
    15961 ctctggtcgt cgtacaccga aatcatcgat gtgaaacagt gctacccgaa cacggcgctg
    16021 gtcggcgtgc aggtggattc agagcagttc ggtaaccagc aggtgagtcg caattatcat
    16081 cttcgcgggc gcattctgca ggtgccgtcg aactataacc cgcagacgcg gcaatacagc
    16141 ggtatctggg acggaacgct taagccagca tacagcaaca acatggcctg gtgtctgtgg
    16201 gacatgctca ctcatccgcg ctacggcatg gggaaacgtc ttggtgcggc agatgtggac
    16261 aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cagtgccgga cggatttggc
    16321 ggcacggagc cgcgcatcac ctgtaacgct tacctgacca cacagcgtaa ggcgtgggat
    16381 gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg gcagacgctg
    16441 acgttcgtgc aggaccgacc gtcggataaa gtgtggacct ataaccgcag taatgtggtg
    16501 atgccggatg atggcgcgcc gttccgctac agcttcagcg ccctgaagga ccgccataat
    16561 gtcgttgagg tgaactggat tgatccggac aacggtcatg agacggcgac agagcttgtg
    16621 gaggacacgc aggccattgt ccgttacggt cgtaacgtca cgaagatgga tgcctttggc
    16681 tgtaccagcc ggggacaggc gcaccgcgcc gggctgtggc tgattaaaac ggaactgctg
    16741 gaaacgcaga ccgtggactt cagcgtcggc gcagaagggc ttcgccatgt accgggtgat
    16801 gtcattgaaa tctgcgatga tgattatgcc ggtatcagca ccggtggtcg cgtactggcg
    16861 gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc gtcctccggt
    16921 accacgctga taagcctggt tgacggaaat ggcaatccgg tcagcgtgga ggttcagtcc
    16981 gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg acggcgttgc agaatacagc
    17041 gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt tccgctgcgt gagtatccgt
    17101 gagaacgacg acggcacgta tgccatcacc gccgtgcagc atgtgccgga aaaagaggcc
    17161 atcgtggata acggggcaca ctttgacggt gaccggcgcg gcacggtgaa tggtgtcacg
    17221 ccgccagcgg tgcagcatct gaccgccgaa gtcaccgcag acagcgggga atatcaggtg
    17281 ctggcgcgat gggacacgcc gaaggtggtg aagggtgtga gcttcctgct tcgcctgacc
    17341 gtggcagcgg acgacggcag tgagcggctg gtcagcacag ccaggacgac ggaaaccaca
    17401 taccgcttca ggcagctggc gctggggaat tacagtctga cagtccgggc ggtaaatgcc
    17461 cgggggcagc agggcgatcc ggcgtcggta tcgttccgga ttgcggcacc ggcagcgcct
    17521 gtcactattg aactgatacc ggggtatttt cagataacgg tggtcccgaa actggctgta
    17581 tatgacccga cggtgcagtt tgagttctgg ttttcggaaa agcggattgc ggatatcagg
    17641 caggttgaaa ccagcgcccg ctatcttggc acggcgctgt actggatagc cgccagtatc
    17701 aatatcaggc cgggccatga ttattatttt tacgttcgca gtgtgaacac cgttggcaaa
    17761 tcggcatttg tggaggccgt cggtcgggcg agcgatgatg cggaaggtta tctgagtttt
    17821 tataaagggt tgatcaataa aacgcatctc ggcaaggagt tgtggacgca gattgataac
    17881 ggtcagcttg cgccggacct gactgaaatc aggacgtcca ttacgaatgt cagcaatgaa
    17941 atcacgcaaa ccgtcaataa aaaactggaa aatcagagtg ctgcaatcca gcagatacag
    18001 aaagttcagg ttgatacaaa taataacctg aacagcatgt gggctgtgaa gctgcaacag
    18061 atgaaggacg gacgccttta tattgcgtgg tatcggtgcc ggtattgaga atacgccagc
    18121 aggaatgcag agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa
    18181 tggcaacaca aagccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    18241 cctgaaacgc ctgacggctc cgaccattac cagcggcggt aatcctccgg cattttccct
    18301 gacacctggc ggacggctga cggcgaaaaa tgccgatatc agcggtaacg tgaacgcgaa
    18361 ctccgggacg ctcaacaacg tcacgattaa taagaactgt cgggttctgg gaaaattgtc
    18421 cgcgaaccag attgaaggcg atctcgttaa aacagtgggc aaacctttcc cacgggactc
    18481 ccgggcaccg gagaggtggc catcagggac cattaccgtc agggtttatg acgatcagcc
    18541 gtttgatcgg caaattgtta ttcccgcggt ggcgtttcgc ggtgctaaac atgagcggaa
    18601 gaataacaat atttattcgt catgccgcct gatagtgaag aaaaacggtg ctgaaattta
    18661 taaccgaacg accctggata atacgctgat atatacgggt gttattgata tgcctgccgg
    18721 tcacggtcac atgacgctgg agttttctgt atcggcatgg ctggtaaatg gctggtatcc
    18781 cacagcaagt atcagcgatt tgctggttgt tgtgatgaag aaatccacag caggtatcag
    18841 tatcagctga attttataac ccagatacgg gcaccagaaa tggtgccttt tttattgcag
    18901 aaaagcgaga ggtaattatg cgtaaacttt atgctgccat tttgtccgca gccatctgtc
    18961 tggctgtatc cggtgcgcct gcatgggcgt ctgaacatca gtccacgctg agcgcggggt
    19021 atcttcatgc ctcgacgaac gctcccggca gtgatgatct gaacgggatt aacgtgaaat
    19081 accgttatga gtttacggac acgctggggc tggtgacgtc attcagctat gccaacgctg
    19141 aagatgagca aaaaacgcat tacagtgata cccgctggca tgaggattcc gtgcgtaacc
    19201 gctggttcag cgtgatggcg gggctgtctg tgcgcgtgaa tgaatggttc agcgcgtatg
    19261 cgatggcggg cgtggcttac agccgtgtgt cgaccttctc cggggattat ctccgcgtaa
    19321 ctgacaacaa ggggaaaacg cacgatgtgc tgaccgggag tgatgacaac cgccacagca
    19381 acacgtctct ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc gtggccattg
    19441 accttgctta tgaaggttcc ggcagtggcg actggcgcac tgacggtttc atcgtgggtg
    19501 tcggttataa gttctgatta gccaggtaac acagtgttat gacagcccgc tggttcaggc
    19561 gggctttttg tggggtgaat atggcagtaa agatttcagg tgtactgaaa gatggggcgg
    19621 gtaaacctgt cgtaaattgt gcgattgaac tgcgggccag aagaaccagt ccgaccgttg
    19681 tggcacacgt tgttgccact tgcgtgacgg acaataacgg tgcttatgtg attgaggctg
    19741 agccggggta ttacgaggtt gcgcttcact gtaacggctg gcagccaacc cgtgtcgggg
    19801 atattgatgt ggcaccgact gatgcaccgg ggacactgaa cgcgtttctg aatgcaccaa
    19861 aggatggtga tttacgtccg gaggtgatga agcgctttga ggaaatggtg gcgcaggcgc
    19921 agcagagtgc cggggc
//
LOCUS       DAEEDS010000076        19698 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEEDS010000076 DAEEDS010000000
VERSION     DAEEDS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.076"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="KLH32_002958"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="KLH32_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="KLH32_002959"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="KLH32_002959"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBE0139705.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KLH32_002960"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KLH32_002960"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBE0139706.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="KLH32_002961"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="KLH32_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBE0139707.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="KLH32_002962"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="KLH32_002962"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBE0139708.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="KLH32_002963"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="KLH32_002963"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBE0139709.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="KLH32_002964"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="KLH32_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBE0139710.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="KLH32_002965"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="KLH32_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBE0139711.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="KLH32_002966"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="KLH32_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBE0139712.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="KLH32_002967"
     CDS             11402..11617
                     /locus_tag="KLH32_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBE0139713.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KLH32_002968"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KLH32_002968"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBE0139714.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="KLH32_002969"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="KLH32_002969"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBE0139715.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="KLH32_002970"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="KLH32_002970"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBE0139716.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="KLH32_002971"
     CDS             complement(16353..16892)
                     /locus_tag="KLH32_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBE0139717.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="KLH32_002972"
     CDS             complement(16908..17426)
                     /locus_tag="KLH32_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE0139718.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="KLH32_002973"
     CDS             complement(17737..17928)
                     /locus_tag="KLH32_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBE0139719.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="KLH32_002974"
     CDS             17946..18098
                     /locus_tag="KLH32_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139720.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..19419
                     /gene="pdeF"
                     /locus_tag="KLH32_002975"
                     /pseudo
     CDS             18280..19419
                     /gene="pdeF"
                     /locus_tag="KLH32_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
     gene            complement(19458..>19698)
                     /locus_tag="KLH32_002976"
     CDS             complement(19458..>19698)
                     /locus_tag="KLH32_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0139721.1"
                     /translation="GLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISV
                     IREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT         4637 a         4795 c         5342 g         4924 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggctataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caatatgagg tgtactggca atagcggaca
    19441 ctaccatttg ttctttttta agcagccatc tgatgatatt tttccctgaa ggctgccggg
    19501 gagatattcc ccagacgaga gtgacgacgc tgacgattgt agaaaatctc aatgtattcc
    19561 cgtattactg agatggcttc atcccggtta ttaaaacgat agtggctcag gctctcattt
    19621 ttcagcgttc cccagaagct ttccatcgga gcgttgtcgt aacagttacc tttacgcgac
    19681 attgatgttt tcagacca
//
LOCUS       DAEEDS010000077        19375 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEEDS010000077 DAEEDS010000000
VERSION     DAEEDS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.077"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="KLH32_002977"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="KLH32_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBE0139722.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="KLH32_002978"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="KLH32_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBE0139723.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KLH32_002979"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KLH32_002979"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBE0139724.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="KLH32_002980"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="KLH32_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBE0139725.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="KLH32_002981"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="KLH32_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBE0139726.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="KLH32_002982"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="KLH32_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBE0139727.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="KLH32_002983"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="KLH32_002983"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBE0139728.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="KLH32_002984"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="KLH32_002984"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBE0139729.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="KLH32_002985"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="KLH32_002985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBE0139730.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="KLH32_002986"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="KLH32_002986"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBE0139731.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="KLH32_002987"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="KLH32_002987"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBE0139732.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="KLH32_002988"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="KLH32_002988"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBE0139733.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="KLH32_002989"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="KLH32_002989"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBE0139734.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="KLH32_002990"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="KLH32_002990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBE0139735.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="KLH32_002991"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="KLH32_002991"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBE0139736.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="KLH32_002992"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="KLH32_002992"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBE0139737.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="KLH32_002993"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="KLH32_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBE0139738.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="KLH32_002994"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="KLH32_002994"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBE0139739.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAEEDS010000078        19263 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEEDS010000078 DAEEDS010000000
VERSION     DAEEDS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19263
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.078"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            32..331
                     /locus_tag="KLH32_002995"
     CDS             32..331
                     /locus_tag="KLH32_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBE0139740.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="KLH32_002996"
     CDS             328..2076
                     /locus_tag="KLH32_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0139741.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="KLH32_002997"
     CDS             2365..2643
                     /locus_tag="KLH32_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139742.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="KLH32_002998"
     CDS             2640..2945
                     /locus_tag="KLH32_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139743.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="KLH32_002999"
     CDS             2955..3116
                     /locus_tag="KLH32_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBE0139744.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3258..3482
                     /locus_tag="KLH32_003000"
     CDS             3258..3482
                     /locus_tag="KLH32_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139745.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="KLH32_003001"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="KLH32_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="KLH32_003002"
     CDS             4980..5552
                     /locus_tag="KLH32_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBE0139746.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="KLH32_003003"
     CDS             5554..6765
                     /locus_tag="KLH32_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBE0139747.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="KLH32_003004"
     CDS             6762..7100
                     /locus_tag="KLH32_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBE0139748.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="KLH32_003005"
     CDS             7097..7393
                     /locus_tag="KLH32_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBE0139749.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="KLH32_003006"
     CDS             7393..7833
                     /locus_tag="KLH32_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0139750.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="KLH32_003007"
     CDS             7817..7999
                     /locus_tag="KLH32_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139751.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="KLH32_003008"
     CDS             8055..8345
                     /locus_tag="KLH32_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBE0139752.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="KLH32_003009"
     CDS             8365..10026
                     /locus_tag="KLH32_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBE0139753.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="KLH32_003010"
                     /pseudo
     CDS             10040..10321
                     /locus_tag="KLH32_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KLH32_003011"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KLH32_003011"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBE0139754.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KLH32_003012"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KLH32_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBE0139755.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KLH32_003013"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KLH32_003013"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBE0139756.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KLH32_003014"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KLH32_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBE0139757.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KLH32_003015"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KLH32_003015"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBE0139758.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KLH32_003016"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KLH32_003016"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBE0139759.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KLH32_003017"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KLH32_003017"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBE0139760.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="KLH32_003018"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="KLH32_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBE0139761.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4727 c         5159 g         4361 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagtaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 tat
//
LOCUS       DAEEDS010000079        17495 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEEDS010000079 DAEEDS010000000
VERSION     DAEEDS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17495
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.079"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(45..776)
                     /gene="rlmB"
                     /locus_tag="KLH32_003019"
     CDS             complement(45..776)
                     /gene="rlmB"
                     /locus_tag="KLH32_003019"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBE0139762.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(956..3397)
                     /gene="rnr"
                     /locus_tag="KLH32_003020"
     CDS             complement(956..3397)
                     /gene="rnr"
                     /locus_tag="KLH32_003020"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBE0139763.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3436..3861)
                     /gene="nsrR"
                     /locus_tag="KLH32_003021"
     CDS             complement(3436..3861)
                     /gene="nsrR"
                     /locus_tag="KLH32_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBE0139764.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4066..5364)
                     /gene="purA"
                     /locus_tag="KLH32_003022"
     CDS             complement(4066..5364)
                     /gene="purA"
                     /locus_tag="KLH32_003022"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBE0139765.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5468..5665)
                     /locus_tag="KLH32_003023"
     CDS             complement(5468..5665)
                     /locus_tag="KLH32_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBE0139766.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5747..6751)
                     /gene="hflC"
                     /locus_tag="KLH32_003024"
     CDS             complement(5747..6751)
                     /gene="hflC"
                     /locus_tag="KLH32_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBE0139767.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6754..8013)
                     /gene="hflK"
                     /locus_tag="KLH32_003025"
     CDS             complement(6754..8013)
                     /gene="hflK"
                     /locus_tag="KLH32_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBE0139768.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8099..9379)
                     /gene="hflX"
                     /locus_tag="KLH32_003026"
     CDS             complement(8099..9379)
                     /gene="hflX"
                     /locus_tag="KLH32_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBE0139769.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9455..9763)
                     /gene="hfq"
                     /locus_tag="KLH32_003027"
     CDS             complement(9455..9763)
                     /gene="hfq"
                     /locus_tag="KLH32_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBE0139770.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9849..10799)
                     /gene="miaA"
                     /locus_tag="KLH32_003028"
     CDS             complement(9849..10799)
                     /gene="miaA"
                     /locus_tag="KLH32_003028"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBE0139771.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10792..12639)
                     /gene="mutL"
                     /locus_tag="KLH32_003029"
     CDS             complement(10792..12639)
                     /gene="mutL"
                     /locus_tag="KLH32_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBE0139772.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12649..13986)
                     /gene="amiB"
                     /locus_tag="KLH32_003030"
     CDS             complement(12649..13986)
                     /gene="amiB"
                     /locus_tag="KLH32_003030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBE0139773.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14005..14466)
                     /gene="tsaE"
                     /locus_tag="KLH32_003031"
     CDS             complement(14005..14466)
                     /gene="tsaE"
                     /locus_tag="KLH32_003031"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBE0139774.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14438..15985)
                     /gene="nnr"
                     /locus_tag="KLH32_003032"
     CDS             complement(14438..15985)
                     /gene="nnr"
                     /locus_tag="KLH32_003032"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBE0139775.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15984..17123
                     /gene="queG"
                     /locus_tag="KLH32_003033"
     CDS             15984..17123
                     /gene="queG"
                     /locus_tag="KLH32_003033"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBE0139776.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17106..17159)
                     /locus_tag="KLH32_003034"
     CDS             complement(17106..17159)
                     /locus_tag="KLH32_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139777.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17392..17467)
                     /locus_tag="KLH32_003035"
     tRNA            complement(17392..17467)
                     /locus_tag="KLH32_003035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17432..17434),aa:Gly,seq:gcc)
BASE COUNT         3965 a         4990 c         4397 g         4143 t
ORIGIN      
        1 aaaaaaccat ccaaatctgg atggcttttc ataattcaga ggaactagct gcgctgacga
       61 accgcttcaa ataagcaaat tccggttgca accgaaacgt tcagggaaga aacacttcct
      121 gccattggga tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg cataccttca
      181 ccttccgcgc ccatcaccag cgccaggcgt ccggtcattt tgctctgata gagtgtatga
      241 tccgcctcgc ctgccgtacc gacgatccag atattctctt cctgcaacat acgcatggtg
      301 cgcgcaaggt tagtcacccg aatcagcgga acgctttctg ccgcgccgca ggctactttt
      361 ttcgccgtag cgttgagctg tgcggagcga tctttcggca caatcaccgc atgaacgcct
      421 gcggcgtccg cactacgcag gcacgcgccg aggttgtgcg gatcggttac gccgtcgagg
      481 atcagcagga acggttgatc gagcgaagcg atcagatccg gcagatcgtt ttcctgatac
      541 tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc gcttttctcg
      601 tcgagatatt ggcggtttgc caactggata accacgccct gagactcaag ggcgtgaatc
      661 agcggtaaca gacgtttatc ttcacggcct tttaaaataa agacttcctg aaaacgttca
      721 ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt aaatcatttc gctcattaat
      781 gtactcgttg tttacgtttt gcctgatgca ctacgtttat ccggcctaca tgatctctgc
      841 aatatattga atttgcgtga ttatgtaggc cggataaggc gttcacgccg catccggcat
      901 gaacaaagcg cattttgtca gcaatctaac cctctttttt taaagagggt attgatcact
      961 ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc tgcggctatt ttctgcgttt
     1021 tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc cgctttcggc ttcgtttttt
     1081 tctcaccgcg gaaggcgctg tctggctcaa agtttacctt tttaccgacc tgacgacgct
     1141 tgccgccttt tttgcctgca tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt
     1201 tgcgcggtgc gcgttcactg gagatcagac taaagtcgat tttgcgctcg tccatattaa
     1261 ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata agtctggccg ctggattccc
     1321 ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc attgtccagc gaagagacat
     1381 ggaccagacc atcaatgaac aagtcgtcca gacggacgaa gaagccaaag ccagtgacgc
     1441 tggaaattac gcctttaaag acgttaccta cctggtcgag catgaagtca cacttcagcc
     1501 agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac
     1561 ccagttgcaa catctcttcc atcgaataat ggtagccgcc ggtttcagtg gtgttgccct
     1621 gatgcccctg ctctttcgcc agcagatatt taatggcgcg gtgcagcgtc aggtcaggat
     1681 aacgacgaat cggcgaagta aagtgcgcat aggactgcaa tgccagaccg aagtgaccac
     1741 ggttttctgg atcgtaaatc gcctgtttca tcgagcgtag cagcatggtt tgcagcattt
     1801 ctgcatcagg acggtcggca accgactcca gcagttccgc gtagtcacgc ggttccggct
     1861 tgttaccacc cggcagctcc agccccagct ccgccagcac tgaacggaaa gaggtaatcg
     1921 cttcggtgct cggcttgtcg tgaatacgga acagtgccgg ttctttcgct ttctcaacga
     1981 aacgcgccgc cgagatattc gccagaatca tgcactcttc aattaatttg tgcgcgtcgt
     2041 tacgctgggt ctgttcgata cgttcaatac ggcgttcagc gttgaaaatg aacttcgctt
     2101 cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc tttatccagc actttataga
     2161 ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta ctgctcgcgc agatcctgat
     2221 cgccctgcag aatatgccag actttggtgt aggtcagacg cgcgtgagag ctcatcaccg
     2281 cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca
     2341 tacacaggcg gtctacctgc gggttgagcg aacacaggcc gttagagagc acttccggca
     2401 gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc acggttacgc gcttctctgt
     2461 ccagcggcgt tggcggacgc acatagtagc tgacgtcggc aatcgcgacc cataaacgcc
     2521 agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc aaagtcacgg gcgtcttcgc
     2581 catcaatggt gaccagcggt aaatcgcgca aatcgacacg gcccgctttt gcttcttccg
     2641 gcacttcttc tttcagcccc gcaacctgtt gctcaacagc ctgcggccag atatacggaa
     2701 tttcatgggt acgcagagcg atatcaaccg ccatgccggt gcccatattg tcgcccagca
     2761 cttcgacgat tttacccacc gctttggtgc ggcgagtcgg acgctgagtc agttcgacta
     2821 cgaccacaaa gcccatccgc gcgcccatga tctgatcggg cgggattaag atatcgaagc
     2881 tcagacggct atcgtcagga accacaaagc cgacgcccgc ttcggtaaag tagcgaccaa
     2941 caatctggct ggtttttggc accagtacgc ggacaatacg cgcttcacga cgacctttac
     3001 ggtcagcacc cagcggctgc gccagcacct gatcgccatg aatgcaggtt ttcatctgct
     3061 cgctggagag atacaaatca tctttacgcc cttcaacccg cagaaagccg tagccatcac
     3121 ggtgaccaat aacggtacct ttcaccaggt cgaggcgttc cggcagcgca tagcactgac
     3181 gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt
     3241 caagctgctc ttcgccttca atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt
     3301 tttcacgttt ggttaaatgt tcgaggataa attcccgact agggatggga ttcgcgtatt
     3361 tttcagcttc gcgttcctgg aaaggatctt gtgacatctc ggttcctccg ttgtcatctc
     3421 tgatgaagat tttcgtcact ccaccagcaa taatttataa agcggttgat tctcttcaac
     3481 caaatcggca agcgtgtagt tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag
     3541 cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac tcactgctgc aattcaccag
     3601 cgataagggc tccagctcgc gcaccacatc accaatacgt atcgcactcg ccggtttacc
     3661 caggcgaatg ccgccatttt ttccacgaac agcagtcacg tagccggcac gactaagttg
     3721 attgattatt ttgaccatat gattacggga gacgccgtag acgtcagtca cttcagaaat
     3781 actggtcatc cgcccttctg gcaatgacgc catgtagatc agcgcacgta atccgtaatc
     3841 agtgaaactc gttaactgca catcaacctc aaaagggaaa tcgggaaaaa atacatttat
     3901 attgatgata aaccagccac aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa
     3961 aagcgaggat ttaacactgt gtcggatagc gtaatggctt aaccgacaca gcaatgtatc
     4021 acactgttag cccggcaggc aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg
     4081 gtcgcgcaga atcatggttt cagtacgatc cggaccggta gagatgatat cgatcggcac
     4141 accggtcagc tcttcaatac gcttgatgta gttcagcgcc gcctgcggca ggccgctacg
     4201 atctttcacg ccgaaagtgg attcagacca gcccggcatg gtttcgtaaa tcggctctac
     4261 acctttccag tcgtcagctg ccagcggagt ggtagtcact tcgcgaccat ccggcatacg
     4321 gtaagccacg cagagtttaa cctctttcag gccatccaga acgtccagtt tagtcaggca
     4381 gaagccagac agggagttca gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc
     4441 ggtacgacga cgacgacccg tagttgcacc gaattcgtta ccctgcttgc agaggaactc
     4501 gccagtttca tcaaacagtt cggccgggaa aggacctgca cctacacgag ttgagtaagc
     4561 tttgaggata cccagaacgt aatcaacata acgcgggccc aggccggaac cggtcgccac
     4621 gccaccagca gtggtgttgg aagaagttac gtacggataa gtaccgtggt cgatatccag
     4681 cagcgtaccc tgcgcacctt cgaacatgac gaaatcgcca cgctgacgcg cctggtcgag
     4741 caggtcagaa acgtcaacca ccatagaagt caggatgtcg gcaacagcca tcgtatcatc
     4801 cagaactttc tggtaatcaa ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg
     4861 atattccatc acttctttca gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac
     4921 acgcagaccg cgacgtgcca ctttatcttc ataagcaggc ccgataccac gaccggtggt
     4981 gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta tccagagcaa cgtgataatc
     5041 aaggatcagc ggacatgctt cagacagcag cagacgctca cgaacgggga tgccacggtc
     5101 ttccagttct ttcatctctt tcatcagcgc ggccggagac agcacaacac cgttaccgat
     5161 gatgctggtt acattctcgc ggagaatacc tgatggaata agatggagaa cggttttttc
     5221 accgttgatt acgagagtat ggcctgcgtt gtgaccgccc tggtagcgta caacatattt
     5281 agcccgttca gtcagaagat cgacgatctt acctttacct tcgtcacccc attgggtgcc
     5341 cagtacgacg acgttgttac ccatttttca aaatcaccgt ttgcttaaaa atggattcta
     5401 ccatcgcttt ttcagagtta cagcactttt tgcactcaaa aatgacctcg atcagtctgt
     5461 tttttgttca gccaatcgtt ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa
     5521 gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt ggtcatcgca gagatcatct
     5581 tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc cagtaccaaa accagggcaa
     5641 gcgccagcca gattgtcgaa ttcattcatt atccttatag aaaaagaaaa ccaccgacat
     5701 ccctgaggat gcggtggctt tattgacctg taccgcagtc gttatattaa cgcgttgcgg
     5761 aagtcggcgt cttcatgtag cggaagaaat cgctatccgg gctcatgacc atcacgtcct
     5821 gattgccaga gaagctgttc tcataagcac gcaggctacg gatgaatgcg tagaagtccg
     5881 gatctttact gaatgcatca gcaaacagtt tggctgcttc ggcatcgcct tcaccacgca
     5941 taatgcggcc ctgacgctca gcttctgcca gcgttctggt cacttcatag tcggcagtcg
     6001 cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg acgacgcgct accgcttcac
     6061 gctcggcgcg catacggttg tagatcgctt cagacacttc ggtcggcagg ttgatctgct
     6121 tgatacgcac atcgacaact tcaataccca gcgccgccat actgttcggg ttgatgaccg
     6181 gaactttgcc cttcgtctca gccgttacgc gctctgccgc ttcggcaatg gcgttatctg
     6241 ctgccggggt agtaacttca tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta
     6301 cttcgagggt cagacgacca cgggaatcgg tgacgatatc tttcacgtcc aggcgaccaa
     6361 tttcagaacg cagacggtca gagaacttac gtttcaacag cacttccgct tgcgaaatgt
     6421 cgccaccacc cgttgccagg tagtaacggc tgaaatcgct gatgcgccat ttgatgtaag
     6481 agtcgacgat caggtctttc ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg
     6541 tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa cggtatcttg aaatgcagac
     6601 caggctcata aaccaaaggt ttgttgtcat cgtcacgcag taccttacca aaacgcagcg
     6661 taataccgcg ctcaccttct ttgacgacaa agacagacat gtaaagcact accagcacga
     6721 tgatgataat cgcgataact gacttacgca tcgttattcc ccctgacgct ggtagtcgtt
     6781 acgctgcgcg ttggcgcggc gttggtccat aatatcgccc tgactggtgg acgacgtgtt
     6841 gcttgctcca ctggttgtgg aggaagaggc tggcggcaga cgcagcagat tgctggcacc
     6901 gttatcgctc ttcgccgcag gggcgttacc acctttcagc atctggtcta acggcagaac
     6961 catcaggttg ccacctttat cgttaaccag cactttgcgg gtgttaccca acactttttc
     7021 catcgtctcg atatacagac gctcgcgagt aatttccggc gcggctttat attccggcag
     7081 aagtttagca aagcgcgcca cttcaccctg agcttccagg atggtctggg ccttgtacgc
     7141 acgcgcctct tcgaggatac gttgcgcctg accgttcgca cgcggctgaa cttcgttggt
     7201 atacgcttct gcttcacgaa tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc
     7261 aaacgccgct tttacttctt ccggcggacg agcagcctgg aagttgacgt ccagcagcgt
     7321 gatacccatg tcatacggac gaatcgtctc ttccagttcg cgctgagtat cgctacgaat
     7381 cacggtacga ccttccgtca gaatgcggtc catggtgtat ttaccgataa ctccacgcag
     7441 ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg gtcacgctat acagatattt
     7501 ttccggattg gtgacgcggt actgcacgtt catctcaacg cgcactacgt tctcgtccga
     7561 cgtcagcatc acaccagaag cggccagttc acgcacggct tccacgttca ccggtttgac
     7621 ttcgtcgata aacgtcggtt tccagttcag acccggctca accagatggc tgaatttacc
     7681 aaagcgtgtt accacgccgc gttcggcttc tttaatggta tagaaaccac tggccgccca
     7741 gataatgaca atcgctgccg ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg
     7801 gccttgcgat gaactgccac cgccagatcc ggtgccttta ccgccgccca gaccaccgag
     7861 ctttttgctc agtttgcgga agatatcatc taaatcaggt ggcccttgat cgcgaccgcc
     7921 tttgtttcca tttccctcag agttgccgcc aggtttgctg cttccccacg ggtcgcggtc
     7981 ttgtccgtta ttaccgggct gattccacgc catgtttgtg ctccatattt gttatgcggt
     8041 gatccctgtt gtctttcgac gccagaggat atgactccac gcttcagacg ctacgccgtt
     8101 agatcaggta atcgatcaac gccggttctt gtttacagag gcgacgccag tcaacgatcg
     8161 gcatacgaac ttgcagactt acgctgccgt cctcctccat ccactctttt tctattgcct
     8221 gaagctgata aaaacgactc ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct
     8281 gcgccacctc gccggaaagc cgctccgtca aagcctgaaa aagctgtggt atccccgctc
     8341 cggtctgtgc ggaaagccag acacggatcg gtttgttctc ttcgtcccga tcaatacgcg
     8401 gttcgaaatc ttccagcata tcgatcttgt tcatcaccag cagggttggg atctcgtgag
     8461 cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg
     8521 ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt ctcttgtaac gtggctttaa
     8581 atgccgccac cagatcgtgc ggcaggtggc gaataaaccc tacggtatct gcaagtacgg
     8641 tttcaccgac atctgcaacg tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact
     8701 ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa aagggtagat ttaccggcgt
     8761 tggtatatcc caccagcgaa acagtaggaa cgtcggcttt gatacgcgat tgccgcccct
     8821 gctcacgctg cttttcaact ctttccaggc gcgactgtat ctgcacgatg cgattacgca
     8881 acaaacgacg gtcggtttcg agctgggttt cacccggacc acgcaaacct atcccgcctt
     8941 tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt agccagatgg cgcagctgcg
     9001 ccagctcaac ctgcaactta ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa
     9061 taaggccggt gcggtcgata acacgacact cgcacaaacg ctccaggtta cgctcttgcg
     9121 ccgggctcag ggcatggtca aaaagaacga ccgaagcacc catcgctttg acagcttccg
     9181 caatttcaac tgctttacct tcacctacaa aatactttgg gtgcggcgct ttacggctac
     9241 cggtaatcac ctgcaatgct tcgacaccgg cggaagagac cagagattca aactcctgga
     9301 ggtcttccat atctttgtct tgcgtaaaat agatgtgtac cagtaccgcc tgctcaccag
     9361 catcataacg gtcaaacaag cgtataaccc tctaaataga tcagcgggga acgcaggatc
     9421 gctggctccc cgtgtaaaaa aacagcccga aaccttattc ggtttcttcg ctgtcctgtt
     9481 gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg
     9541 cgttgttact gtgatgagaa accgggcgag atgggacaac agtagaaatc gcgtgcttgt
     9601 aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt
     9661 gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca
     9721 gtgcgttcag gaacggatct tgtaaagatt gccccttagc cattctctct tttccttata
     9781 tgcttatttg tactttgaac ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt
     9841 gtacacattc agcctgcgat agcaccaaca acctgtaata cttcgtcacg cgcctgttct
     9901 ggtttttcac tgtcaagcca gtgaacccct tcccaaccac gcagccaggt tatctgccgc
     9961 ttcgccaact gtctcgtggc gcaaacacct cgataaacca tttcatcgta tgagatttcg
    10021 ccttcaaggt aagaccacat ctggcgataa cccacgcaac gaatggaagg caagtccgta
    10081 tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt caaaacctga agccaacatc
    10141 tgatgaaaac gctgctcaat gcgttgatgg agcagttcac ggctcgccgg ggcgatggcg
    10201 aactgatgca cctgatacgg tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt
    10261 ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc atttggatga
    10321 atccttgctg ccgcaaccgg atctacctcc tgaagttgac gatgcaatga ctcccaacct
    10381 tgctctgccg cctgttgctc aattctggcc cgcacttccg ggtctgccga cggtagcggc
    10441 gacaaccctt ccagcaatgc cttgaaatac aacatcgtac cgcccactaa cagtgggatc
    10501 cgccccgccg cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc
    10561 gagtaagcct gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc gagtaactct
    10621 tcagcgttcg gcttcgccgt cccgataacc atccctttgt aaataagggc agaatcaacg
    10681 cttatcaact ctactggtaa aattttacgc agctcaatgg ctaacgccgt tttaccggag
    10741 gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat atcactcatc
    10801 tttcagggct tttatcgccg gatgtaaatc aacagattgt aacagaccac ccggcggcgt
    10861 tttcacaagt tgcggacata accgttccac gtccgccagc agggttatgg cctgtgccat
    10921 tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg
    10981 ttcgaatacg gactgcttcg ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt
    11041 ttgttggcgt aagggtaaag gcacggccct gatggtcaca tgctgtgcat ctgactggaa
    11101 atcaataccc aattccgcta gggcagactg cgctttttct aatgccgatt tttcttcgcc
    11161 agaaactttt agccgcaacg gaatcagcag cggctgggcg caaacgggcg cttcacccgg
    11221 cgtcaattgt gcctgacgca gccaacgttc tgccactggc aaggataaaa gtgaaatgtt
    11281 gccatcgcgc tccagcaacg cacagtcgga atggacgata gtcagtaccc gaccaaaact
    11341 ctgactgttc gccgcaagtg caggttcctg cggttccggc gcttttggtt tttgcatcgg
    11401 cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt agcctggctg
    11461 cgcattcggc cagggggcag ccggacgact gcctgaggcg ggcgcaggag agtagcgcgg
    11521 agctaccggc tcacgagctg ccggttctgc aaagtgattg cgccccgccg ccacgcggtt
    11581 ttccgggatg gcacgcggtg cgggttgtgg ttcatcgtcc agcggtagcg gcgtttccag
    11641 ttgctgttgt agcacgctca gcacgccctg atagataaaa tcatgcacca gacgcgactg
    11701 atggaaacgc acttcgtgtt tggcggggtg tacgttgacg tccacctgat gcgggtcgat
    11761 ctccagatac aacacaaatg ccggttgctg atcggccccc agtttgtctt cgcaggcctg
    11821 gcggatcgca tgattgatca ggcgatcgcg catcatgcga ccattcacgt agcaatactg
    11881 aatttccgcc agtgcgggcg tagtgtgatt tggatcggcc acccagccgc gcagggtgag
    11941 atcgccgtgt tgccattcaa tcgccagcgc ttgttcgaga aaagcggtgc cgcaaatcgc
    12001 gcctaagcgc cgttcttttt gcccgccttc cggcactgcg cggtactgac gcacaatttt
    12061 accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat
    12121 ttcatcaata tggctaaatt cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt
    12181 gttgtagaac agatccagca cctccagcgt cgttcccacc ggatgcgctg ccggtttaac
    12241 cgtcacgtcc atgtcgcgtc cttcggcata ggcctgccag gcttcctgct gttctgcggt
    12301 gcgtgaagtg agcgtcaggc gggaaaccga actgatactc gccagcgcct caccgcgaaa
    12361 gcccaggctg ataatggctt cgagatcgtc cagagaggcg attttactgg tggcatgacg
    12421 agccagcgcc agcgccagct catctttttt gataccgcag ccgttatcac gaatgcggat
    12481 aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg catcgaggct
    12541 gttttccact agttctttga ctaccgacgc aggtcgctcg accacctcac ctgcggcaat
    12601 ctggttcgcc agttgtggcg gtaagacctg aattggcatc aatcgtcctt agtttggcag
    12661 cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc gttgcaccct gcggcgcaga
    12721 ttgcatcgga tgcgcaagga agtaattgcg caggcctttg taaatggctt ctgccagctg
    12781 ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt
    12841 ttcgaccagt actgatggga tatccggcga gcgcagaacg ccaaggctgg cgtgttctgg
    12901 tcgacgttta tgtatttcgc caatgcgttg caactgactg atcatactgg tcgctacatc
    12961 ataccctacc cgctgggaat gaccgaactg taaatccagc accgcctggc ttaaataggg
    13021 gtcagactga ctgttcgcca gcacatcacc cgccccaccc agcagctccg acagtttctc
    13081 gtactgctcc agccagctgg ccatttcact gttggcgcga cggttagaga gcacccatac
    13141 ggaagcgcca gtcgcactac ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt
    13201 ggcgttttgc ttatgtgcca catcactgcg ccccatcacc gagataaagt aatccccgtc
    13261 acgggttaaa acgcctttaa acatcggatc gtcattgagc aaagtacgca atttacgcgc
    13321 gatggcgatg gtgacatttt tctcccgcgt accaccgggg ccgatagcgc cagggtcctg
    13381 accgccgtgt ccggcatcaa tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg
    13441 cgctgccgga cgcgttaccg tattactgct gataacaccc gtagtgcggt tactttccgt
    13501 tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgcag gcgtttcaac
    13561 gcgtttcgca accacgggcg gcggaggagg cggtggcggc acatcggcgt taatcgtaaa
    13621 gacgacagtg taattgctgc cattctgccg cttcaccgct tcggttttac cgttttcggt
    13681 aagatcgacc accagccgta gcgtttgtgc atctttaggc gttccagagc gaatcgcctt
    13741 caccagatta ttgccgctga acaacaacgg cagtccctga atcacgcccg tttgtttgat
    13801 atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca taatcaggat cgccaataaa
    13861 actcaacgtt atccgcgcct gttgattacc gttagaaacc tgaatatcag agagcgtcgc
    13921 ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc aaccaatttc tgatgcgata
    13981 catcatcccg ccacctttca aaggttaacc ggctaaacgc gccagcaaca attcacccgc
    14041 agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc
    14101 gacatccggg tcaggaagaa cacctgtacc ttgttgtggc cactccacca ggcagatggc
    14161 atcgttggca aaataatcgc ggatccccat aaactccagc tcctcgggat cggcaaggcg
    14221 gtacaaatca aagtgataga ccattaagtt gtcgagcgta tagggttcga ccagcgtata
    14281 agtggggctt ttgacattac cctgatgacc cagagcctgt aaaaagcccc ggctaaaggt
    14341 ggttttacct gcgcctaaat cgccatacag atagattacg gttgcgccat cgcaggcttt
    14401 cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca gggagcggaa ttactcgatt
    14461 catcatggtt tttatcagtc acttccgggt taacaatacg ctgtagcgtg gaaaagagat
    14521 cggttgccag catcccgcgc gttccaaaac gcgccgccag tacgtcagct gccgcaccgt
    14581 gcgcgacaca gcctgcacag gctgcatcat acggcgacag tttttgccca agcaatgcgc
    14641 caataatacc agagagcaca tcgcccatgc cgccgctcgc catgcctgca tttccggcat
    14701 caataatgcc taaagcgtca ggatgggcgg cgaccacggt tccggcacct ttcagcaccg
    14761 ctacgccgcc ataacgttgt accagacgtt tggcgcaatg taagcggtca ctttcaattt
    14821 cagcgacgga acagcctaac aaccgtgcgg cctcgctagg atgcggcgtg atcacgcgat
    14881 tgtgacgctt atcgggattg attgccagca ggttcaatgc atcggcatcc cacaacatcg
    14941 gtttgcgaaa attctcaact ttttgcaggg cttttttccc ccactcttgc tggcccagac
    15001 cgggaccaat caccaccaca tcggcccatt ccaggctttc ggtaagagag tccatagtca
    15061 gttcatgcac catcaattct ggtcgtgcag tcagcagcgg cgcaatgttc tcactgcggg
    15121 tcagtactcg gactaaacca gcaccagcac gtagcgccgc ttcccccgtc atacgaatag
    15181 cccccgccgt gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg cctttatgcg
    15241 aagtcgggcg acgcggtttc agccagtgag aaagttgttc tgccgaaaac cgctgaattt
    15301 tcgtctcctg acctgccagc cagctatcca gccccagtga gtcaaaatgc agttgtcctg
    15361 taacatcccg cgcttttcca gtgagcaagc ctagtttcag cgcaataaaa gtgatggtgt
    15421 gatcggcgtt gatcactgcg cctggcgtag cgccggtttc agccagcagg ccggaaggga
    15481 tatcaaccgc cacaatcggc gcaggatggg aattagcgtg gtcgattaac tggctaatgg
    15541 attcgcgggg cgcttgctgc aagccggtac cgagcagcgc atcaacaatc agatctaccg
    15601 attcgggcca gacaatattc gaagcatgga tctcgccacc cgcgtttaac catgcttcgc
    15661 gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc ctgggccaac aacgtgacct
    15721 caatgccgac cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg ttattaccat
    15781 gaccgcacag caccagccag tggcgggcgt caggatacgc cgaacgacac acctggaatg
    15841 cggcctcgcc agcgcgaagc atcagctcat agagtgtgag ccccagcaca tctgccgcct
    15901 cgcgttctcc gcggcggata tcgtcggcgt accagacggt gtgtggtata cttacggggt
    15961 ttttcttcat tgtatggtcc gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa
    16021 aaattaaaca gtgggggctg gaactgggct ttcagcaggt aggtattacc gataccgatc
    16081 tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa acaataccac ggcgaaatgg
    16141 actggatggc acgtcacggt atgctgcgcg ctcgccccca tgagttatta cccggtacgc
    16201 tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt gccagcacgc
    16261 tgaaaaaccc caaactcggc tatgttagcc gttatgcgct gggccgtgac tatcacaaac
    16321 ttctgcgcaa ccgactcaaa aagctgggcg agatgattca gcaacattgt gtttcgctga
    16381 attttagacc gtttgtcgat tctgcgccta ttctcgagcg cccgttagct gaaaaagctg
    16441 ggctcggctg gacaggtaag cactcactta tcctcaatcg cgaggccggt tcgttcttct
    16501 ttttaggcga attgctggtc gatattccgc tgcccgtgga tcaaccagtc gaggaaggat
    16561 gcggcaaatg cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc gagccatata
    16621 ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga acttgaaggg gcgctcccgg
    16681 aagagttgcg accgttaatg ggaaaccgta tttacggttg cgatgactgc cagcttatct
    16741 gcccgtggaa tcgctattcg caactcacta cagaagacga tttcagcccg cgtaagccgc
    16801 tacacgcacc ggaactcatt gagttatttg cctggagcga agagaagttt ttaaaagtca
    16861 cggaaggatc ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat atcgccgtgg
    16921 cattaggcaa tgccccttgg gatgaaacga ttttgacagc gctggaaagt cgtaaaggtg
    16981 agcacccact tctcgatgag cacatagcgt gggcgattgc gcagcaaata gagagacgaa
    17041 atgcgtgcat agtcgaagtg caattgccga aaaaacagcg tctggttcgg gtgattgaaa
    17101 aagggttacc gcgtgacgcc tgattcatcc acagcttgtg aataaaaata aaaacgcatt
    17161 gcaattcaag acgaataaaa tcgtcaagtg atcgactcga caaaacggaa tcttgatttt
    17221 taacttattt atcaataggt taaaaaatac cattcaccgt gcgaagtttc tttgaaatta
    17281 agattacagc ctgaggctgt ggataactct gtttacaaga gtttgtcaga gacaacaaaa
    17341 accagcaata acgcatcgcg tcgctgtaga tattttattg agagaagaat ttggagcggg
    17401 aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta
    17461 ttcccgcttg ggtggtctgt gccttacagc acttt
//
LOCUS       DAEEDS010000080        17449 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEEDS010000080 DAEEDS010000000
VERSION     DAEEDS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.080"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..164)
                     /locus_tag="KLH32_003036"
     CDS             complement(<1..164)
                     /locus_tag="KLH32_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0139778.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDL"
     gene            complement(356..610)
                     /locus_tag="KLH32_003037"
     CDS             complement(356..610)
                     /locus_tag="KLH32_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBE0139779.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            920..2692
                     /gene="aspS"
                     /locus_tag="KLH32_003038"
     CDS             920..2692
                     /gene="aspS"
                     /locus_tag="KLH32_003038"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBE0139780.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2810..3262
                     /gene="nudB"
                     /locus_tag="KLH32_003039"
     CDS             2810..3262
                     /gene="nudB"
                     /locus_tag="KLH32_003039"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBE0139781.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3291..4031
                     /locus_tag="KLH32_003040"
     CDS             3291..4031
                     /locus_tag="KLH32_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0139782.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4066..4587
                     /gene="ruvC"
                     /locus_tag="KLH32_003041"
     CDS             4066..4587
                     /gene="ruvC"
                     /locus_tag="KLH32_003041"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBE0139783.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4589..5191)
                     /locus_tag="KLH32_003042"
     CDS             complement(4589..5191)
                     /locus_tag="KLH32_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBE0139784.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            5260..5325
                     /gene="yobI"
                     /locus_tag="KLH32_003043"
     CDS             5260..5325
                     /gene="yobI"
                     /locus_tag="KLH32_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBE0139785.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5464..6075
                     /gene="ruvA"
                     /locus_tag="KLH32_003044"
     CDS             5464..6075
                     /gene="ruvA"
                     /locus_tag="KLH32_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBE0139786.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            6084..7094
                     /gene="ruvB"
                     /locus_tag="KLH32_003045"
     CDS             6084..7094
                     /gene="ruvB"
                     /locus_tag="KLH32_003045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBE0139787.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7241..8026)
                     /gene="znuB"
                     /locus_tag="KLH32_003046"
     CDS             complement(7241..8026)
                     /gene="znuB"
                     /locus_tag="KLH32_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBE0139788.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(8023..8778)
                     /gene="znuC"
                     /locus_tag="KLH32_003047"
     CDS             complement(8023..8778)
                     /gene="znuC"
                     /locus_tag="KLH32_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBE0139789.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8857..9789
                     /gene="znuA"
                     /locus_tag="KLH32_003048"
     CDS             8857..9789
                     /gene="znuA"
                     /locus_tag="KLH32_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBE0139790.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9805..11127
                     /gene="mepM"
                     /locus_tag="KLH32_003049"
     CDS             9805..11127
                     /gene="mepM"
                     /locus_tag="KLH32_003049"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBE0139791.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            11247..12218
                     /gene="lpxM"
                     /locus_tag="KLH32_003050"
                     /gene_synonym="msbB"
     CDS             11247..12218
                     /gene="lpxM"
                     /locus_tag="KLH32_003050"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE0139792.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12349..13791)
                     /gene="pyk"
                     /locus_tag="KLH32_003051"
     CDS             complement(12349..13791)
                     /gene="pyk"
                     /locus_tag="KLH32_003051"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBE0139793.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKDY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13919..14788)
                     /gene="hexR"
                     /locus_tag="KLH32_003052"
                     /gene_synonym="yebK"
     CDS             complement(13919..14788)
                     /gene="hexR"
                     /locus_tag="KLH32_003052"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBE0139794.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15125..16600
                     /gene="zwf"
                     /locus_tag="KLH32_003053"
     CDS             15125..16600
                     /gene="zwf"
                     /locus_tag="KLH32_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBE0139795.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16835..>17449
                     /locus_tag="KLH32_003054"
     CDS             16835..>17449
                     /locus_tag="KLH32_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBE0139796.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPVMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRM"
BASE COUNT         4448 a         4307 c         4539 g         4155 t
ORIGIN      
        1 ttcaggtcag atattagcga cctgagtttg ttttcgtcgt tgggtaatgc tttatcgaac
       61 aggcgtttac ctgaacgatt aatggcaaca gcgtgatgcg tatctttacc gacatcaacg
      121 ccgataaaga cctggacgga ttcgtaatcg ctggattcgg tcattctgtc tcccttgtat
      181 atgggttaac cagataacca cggggagcag gtaccggcat ccacgttaca gacggtcccg
      241 gcaaaagtgc ctgacctgac ccctattagc ggttaccagc gccccaccag acccggtgac
      301 atcacccccc ggatcatgga cgactggggg cagtaatcat gccgggtctg gctggctaac
      361 accccattat aaggggtacg aataaagtaa cgggggcatc aaccggctgt tttaatgctt
      421 cggcgtaatc ggcagaccag ccaactcgca ccagaaacac gggctgaccg ctggcgcgaa
      481 atttcgccgc cagcttcccg gcgcgattaa ctacctcatc ggcagtatgt ggaccaccgg
      541 caaaaggtaa gatgccttct tgtaaatcga tcaccaccaa cgcagtggtt ttagcattaa
      601 gttcaagcat gctgactcct gaaaaaacaa aatcgtgcct cacaccttaa tgcgattagc
      661 cgatggcgga acgacaaatt ttgttaattt ttatgagttc gggcaacacc tttatagcaa
      721 actggcgtag agtctgattt caggcccgat ataaagtggt aacgaggcgt tccagcgcgg
      781 gccaggctct tacggtgcgg cagaatttcc agtataatag ccgccttttt tcattcagtt
      841 gtgacataca gctaacgctg cgacttggtc acctgcgcag cagggggcat ccgccggcag
      901 ctaagttaag ggatatctca tgcgtacaga atattgtgga cagctccgtt tgtcccacgt
      961 ggggcagcag gtgactctgt gtggttgggt caaccgtcgt cgtgatcttg gtagcctgat
     1021 cttcatcgat atgcgcgacc gcgaaggtat cgtgcaggta tttttcgatc cggatcgtgc
     1081 ggacgcgtta aagctggcct ctgaactgcg taatgagttc tgcattcagg ttacgggcac
     1141 cgtacgtgcg cgtgacgaaa aaaatattaa ccgcgatatg gcgaccggcg aaattgaagt
     1201 gctggcgtcc tcgctgacta tcatcaaccg cgcagatgtt ctgccgcttg actctaacca
     1261 cgtcaacacc gaagaagcgc gtctgaaata ccgctacctc gacctgcgtc gcccggaaat
     1321 ggcgcagcgc ctgaaaaccc gcgctaaaat caccagcctg gtgcgccgtt ttatggatga
     1381 ccacggcttc ctcgacatcg aaactccgat gctgaccaaa gccacgccgg aaggcgcgcg
     1441 tgactacctg gtgccttctc gtgtgcacaa aggtaaattc tacgcactgc cgcaatcccc
     1501 acagttgttc aaacagctgc tgatgatgtc cggttttgac cgttactatc agatcgttaa
     1561 atgcttccgt gacgaagacc tgcgcgctga tcgtcagcct gaatttactc agatcgatgt
     1621 ggaaacttct ttcatgaccg cgccgcaagt gcgtgaagtg atggaagcgc tggtgcgtca
     1681 tttgtggctg gaagtgaagg gcgtggatct gggcgatttc ccggtaatga cctttgcgga
     1741 agcagaacgc cgttatggtt ctgataaacc ggatctgcgt aacccgatgg aactgaccga
     1801 cgttgccgat ctgctgaaat ctgttgagtt tgcagtattt gcggggccgg cgaacgatcc
     1861 gaaaggccgt gttgccgctc tacgtgttcc gggtggcgca tcgctgaccc gtaagcagat
     1921 cgacgaatac ggtaacttcg ttaaaatcta cggcgcgaaa ggtctggctt acatcaaagt
     1981 taacgaacgc gcgaaaggtc tggaaggtat caacagcccg gtagcgaagt tccttaatgc
     2041 agaaatcatc gaagccatcc tggatcgtac tgccgcgcaa gatggcgata tgattttctt
     2101 cggtgccgac aacaagaaaa ttgttgccga cgggatgggc gcactgctcc tgaaagtggg
     2161 taaagacctt ggtctgaccg acgaaagcaa atgggcaccg ctgtgggtta tcgacttccc
     2221 gatgtttgaa gacgacggtg aaggcggcct gacggcaatg caccatccgt tcacctcacc
     2281 gaaagatatg actgctgcag aactgaaagc tgcaccggaa aatgcggtgg cgaacgctta
     2341 cgatatggtc atcaatggtt acgaagtggg cggtggttca gtacgtatcc ataatggtga
     2401 tatgcagcag acggtgtttg gtattctggg tatcaacgaa gaggaacagc gcgagaaatt
     2461 cggcttcctg ctcgacgccc tgaaatacgg tactccgccg cacgcaggtc tggcattcgg
     2521 tcttgaccgt ctgaccatgc tgctgactgg caccgacaat atccgtgacg ttatcgcctt
     2581 cccgaaaacc acggcggcag cgtgtctgat gactgaagca ccgagctttg ctaacccgac
     2641 tgcactggct gagctgagca ttcaggttgt gaagaaggct gagaataact gatatgactc
     2701 aaatacacga aatcattcgc gttgcatcaa ggcggcaact gagtgaactc ccatgagcat
     2761 agataactat gtgaatggga tgagcgaagg cagtcaacga agaggcagcg tgaaggataa
     2821 agtgtataag cgtcccgttt cgatcttagt ggtcatctat gcacaagata cgaaacgggt
     2881 gctgatgttg cagcggcgtg acgatcccga tttctggcag tcggtaaccg gcagcgtgga
     2941 agagggtgaa accgcgccgc aagctgccat gcgcgaagta aaggaagagg ttaccattga
     3001 tgttgtcgct gaacaactga ccttaattga ctgtcagcgc acggtagagt ttgaaatttt
     3061 ttcacattta cgtcatcgct atgcgccggg cgtgacgcgt aatacggaat catggttctg
     3121 tcttgcgctt ccgcacgagc ggcagatcgt tttcactgaa catctggctt acaagtggct
     3181 tgatgcgcct gctgcggcgg cgctcactaa gtcctggagc aaccggcagg cgattgaaca
     3241 gtttgtaatt aacgctgcct gaacaggcag ggctatctgg agatattttt atggcaggtc
     3301 atagtaaatg ggccaacacc agacatcgta aagctgcgca ggatgctaag cgcggtaaaa
     3361 tcttcactaa aatcattcgt gagctggtaa ccgcggctaa gctgggcggt ggcgatccgg
     3421 acgctaaccc gcgtctgcgt gcggcggtgg ataaagcact gtctaacaac atgacccgtg
     3481 acacactgaa ccgcgcaatt gcacgtggtg tgggcggtga tgatgatgca aacatggaaa
     3541 ccatcatcta cgaaggttac ggtcctggcg gcacggcaat catgattgaa tgtctgtctg
     3601 acaaccgcaa ccgtaccgtt gctgaagtgc gtcacgcatt cagcaaatgt ggcggtaacc
     3661 tcggcactga cggttccgta gcttatctgt tcagcaaaaa aggcgtgatc tccttcgaga
     3721 aaggcgacga agacaccatc atggaagcag ctctggaagc aggtgctgaa gacgttgtga
     3781 catatgatga cggcgcgatt gatgtctaca ctgcatggga agaaatgggt aaagtgcgcg
     3841 acgctctgga agcggcaggt ctgaaagcag acagcgcgga agtttccatg atcccgtcca
     3901 ccaaagctga tatggatgca gaaaccgcgc cgaaactgat gcgtctgatc gatatgctgg
     3961 aagattgcga cgacgtgcag gaagtttacc ataacggtga aatctctgat gaggttgcag
     4021 cgactctctg atgatgcctg ctaaacagca aaacggagac gcgtgatggc tattattctc
     4081 ggcattgacc cgggttcgcg cgtgaccggc tacggcgtca tccgccaggt aggtaggcaa
     4141 ctgtcctacc tgggtagcgg atgcatacgc accaaagtgg atgatttacc gtctcgtctg
     4201 aagctcatct atgcgggcgt gacggaaatc atcacccagt tccagcctga ttatttcgcc
     4261 attgaacaag tctttatggc gaagaacgct gactcagccc tgaaactggg ccaggcgcgc
     4321 ggcgtggcga ttgttgcggc ggtgaatcag gaattgccgg tatttgaata cgcggcacgt
     4381 caggtaaagc aaacggtggt aggtatgggt agtgccgaaa aaagccaggt gcagcatatg
     4441 gtccgcacct tgctgaaact gcccgctaat ccacaggcgg atgccgccga tgcgctggcg
     4501 attgccatca cccactgcca cgttagtcag aatgcgatgc agatgagcga atcgcggctg
     4561 aacctgacga gagggcgact gcgttaagtt ataccgcctc ggtcagttcc ggctgaggcg
     4621 tttcaactcc ctccgcgtga atcaacacca aacccggatg atgccacaaa ctggctggcg
     4681 tgacggtttt acgctcccac ggaatagaac ctaagaacca gaatttccag aaagtgagtt
     4741 ttgcattcgg attgctattt aacagttgtc caaacgtctc tatttcaccc accggaatac
     4801 atagcgcctc tttataaata tcaaagacaa attttgaaca gaactggcgc gaagattcgt
     4861 atttaaaacc cgtgtggtaa agtttgcgta gccgggaggg aacctgttca acaattcgtt
     4921 gtttttgttg ttctgtcagc ccagcgtcta atcgctttat agcatagcgt tgattagacg
     4981 aacgtttaat aaaacgagat agcgtggtga tggttgaaag gggaacccgg ctttctgcaa
     5041 ccaggaagtc ttcaccgtta tgaccgataa tgatcccgac gtgattactc cagcaatttg
     5101 atgcagctga tatttgacca aataaggcag cacctataca tgtaaagacg atatctccaa
     5161 tttcatattc agcagggtaa ttaatattca cgttgtcatt ccattgaaat agatacgaat
     5221 atacgtaata gcataaaaaa ttatttttca ggagtcgcaa tgtatatttt tattacgcat
     5281 ttcttcactg aatatgtaat attcaaatat ttgcttccaa tataacctgt tgaataaatt
     5341 atactgtgcc atttttcaga tcatcgggac acctcgcaag ttttcttcat ccttcgctgg
     5401 atatctatcc agcatttttt tatcatacag cattatcttt gattcattac gcaggagcgt
     5461 catgtgatag gcagactcag aggcatcatc attgaaaaac aacccccgct ggtgttaatt
     5521 gaagtgggcg gcgtaggcta tgaagtgcat atgccgatga cctgttttta tgaactccct
     5581 gaagcgggtc aggaagcgat cgttttcacc cactttgtgg tgcgtgaaga cgcgcaactg
     5641 ctgtacggtt ttaacaataa acaagaacgc acgttgttca aagagttgat caaaaccaac
     5701 ggcgtcggcc cgaagttggc cctggcgatc ctctccggaa tgtcagcgca gcagttcgtt
     5761 aatgccgttg agcgtgaaga agtgggggca ctggtgaaac tgccgggtat tggcaaaaaa
     5821 accgccgaac gcttgattgt tgaaatgaaa gaccgattta aaggtttgca tggcgatctc
     5881 tttacgccag ccgccgacct ggtactcacg tctcctgcta gcccggcgac cgacgatgct
     5941 gaacaagaag cagttgccgc gctggtggcg ctaggctata aaccacaaga agcaagccgc
     6001 atggtgagca aaatcgctcg ccctgacacc agcagtgaaa cattaattcg cgaagcccta
     6061 cgcgccgcgt tatgaggtaa aggatgattg aagcagaccg tctgatttct gccggtacca
     6121 ctttgccgga agatgtagca gatcgcgcca ttcgccctaa attactggaa gagtatgttg
     6181 gtcagccgca ggttcgttca cagatggaga ttttcatcaa agcagcgaaa ctgcgcggcg
     6241 atgccctcga tcatttgttg atttttggtc ctccggggtt gggtaaaact acgcttgcca
     6301 acattgtcgc caatgaaatg ggcgttaatt tacgcacgac ttctggtccg gtgctggaaa
     6361 aggcgggcga tttggctgcg atgctcacta accttgaacc gcatgacgtg ctgtttattg
     6421 atgagatcca ccgtttatcg ccagttgttg aagaagtgct gtacccggca atggaagact
     6481 accaactgga tatcatgatt ggtgaaggtc cggcggcacg ctccattaaa attgatttgc
     6541 cgccgtttac cctgattggc gcaaccacgc gcgcaggttc gctgacatca ccgttgcgcg
     6601 accgttttgg tattgtgcaa cgtctggagt tttatcaggt gccggatctg caatatatcg
     6661 tcagtcgcag tgcacgcttt atggggcttg agatgagtga tgacggtgcg caggaagtag
     6721 ctcgtcgcgc tcgcggtacg ccacgtattg ccaaccgtct gctgcgtcga gtgcgtgatt
     6781 tcgccgaagt gaagcacgat ggcaccatct cggcagatat cgctgctcag gcgctggata
     6841 tgttgaatgt cgatgctgaa ggtttcgatt atatggaccg caaattgttg ctggcggtaa
     6901 tcgataagtt ctttggtggg ccggtaggtc tggataacct ggcggcagcc attggcgaag
     6961 aacgtgaaac cattgaagat gtactggaac cttatttgat tcagcaaggc tttttgcagc
     7021 gtacaccgcg cgggcgtatg gcgacgacgc gggcgtggaa tcactttggc ataacgccgc
     7081 cagaaatgcc gtaagtcgga ttggtcgctc gcgccgcatc cgacactggc agtgtgtctg
     7141 atgcgacact gttcgcgtct tatcaggcca gccagagata gcgaaccgta ggtcggataa
     7201 ggcgctcgcg ccgcatccga caaatgtgtt cagcgataga ttagctggcc tgctttttca
     7261 tcatactgat aataaataac agtgccgcac atagcaccac cgacggaccc gccggcgtat
     7321 cgtaaaacgc ggaaaaggtt aaaccgccag ttactgccac catccccacc aaaacagcga
     7381 caccagccat ctgttccggc gtacgggcaa agcgacgcgc agtagctgca ggaataatca
     7441 gcaacgaagt aataatcaac gcaccgacaa atttcatcgc tacaccaatc gtcaatgccg
     7501 tcaccagcat caacaacaat ttcacttgct gtaatttcac accatcaaca aacgccagat
     7561 ccgggctaat cgtcatcgac agcaaattgc gccattgcca gaacaaaata gccaccacga
     7621 tgaccacgcc aatcgcaata gagatgagat cttctggcgt cactgccagt aaatcaccga
     7681 acaggtaagc catcaaatca acacgaatgt tagacatcag actaacgacc actaggccca
     7741 gcgacagggc actgtgcgcc ataatcccta ataacgtgtc gatcgccagc tgtggacgct
     7801 tctccagcca taccagaccg cccgccagca gcagcgtaac ggcaatcacc gcatagaatg
     7861 gattcacgtc cagcaacaaa ccaaacgcga caccaagtaa tgaggcatga gccagcgtat
     7921 caccgaaata agacatacga cgccagacta caaacgaacc cagcggaccc gcggcacagg
     7981 cgagcatgat cccggctaac caaccgggaa ataataattc aatcatgagc gatcatttcc
     8041 ccgacgcaaa acaattcgtc cctgtaaatc gtgacgatga ttatgatgat ggcgatagat
     8101 acccagttgt tcagcaccac gaggaccaaa cattgaaata aactccggat gcagggaaac
     8161 aacttccggt gtgccggaac aacaaatgtg gtgattcagg cacagcactt catcggtttt
     8221 tgccatcacc agatgcagat cgtgagaaac cattaaaacg ccacaatcca gttcacgacg
     8281 caactggtca ataaggtcat ataacgccac ttggccattt acatccacgc cttgagtggg
     8341 ttcatccagc actaataatt gcgggcaatt taacaatgct cgcgctaata gtacacgctg
     8401 cgtttcgcca ccagagagct tttgcatcgg tgcgttaatc aggtgcccgg cctggacacg
     8461 tttcagtgca ggcaaaatat cttctttatg tgtaccaggg cgtaagcgta aaaaacggtt
     8521 tacggtcagt ggcaacgtgg tgtcgagata cagcttctgc ggtacatagc cgatgcgcag
     8581 ttttccgttg cgcttgataa ccccttcatc gggtgttacc agcccgagca ctacccgtac
     8641 cagtgtcgac ttacctgcgc catttggccc aagtaaagtc aaaatttttc caggtttaag
     8701 ttccagcgac acatcagaga ggacgcggcg ttggccaaaa gaaaccgaga cattttccag
     8761 ggaaaccaga cttgtcatgt taattttagt cttgcagtag tcatgaaatg ttataatatc
     8821 acacttctca tattcattac gatgattggt cgcattatgt tacataaaaa aacgcttctt
     8881 ttcgcagcat tatccgccgc tctctgggga ggtgcaacac aggccgcaga tgccgccgtt
     8941 gtcgcttcgc ttaaacccgt tgggttcatc gcttctgcca ttgctgatgg tgtaacagaa
     9001 acggaggttt tactgcctga cggcgcttca gaacatgatt attcactgcg tccatcggat
     9061 gtaaaacgct tacagaacgc ggacttagtc gtttgggttg gcccggagat ggaagcgttt
     9121 atgcaaaaac cggtaagcaa attaccagaa gcgaagcagg taacgattgc gcagcttgaa
     9181 gatgtgaaac cgctactgat gaaaagtatt cacggcgatg atgatgatca cgaccacgcg
     9241 gaaaaaagtg acgaagatca ccatcacggc gatttcaaca tgcatctttg gctttcccca
     9301 gagatagcgc gggctacagc ggttgcaatc catggaaaat tagtggaact tatgccgcaa
     9361 agtcgagcca aacttgacgc caacctgaag gattttgagg cacaattggc ctcaaccgaa
     9421 acgcaggttg gtaacgagct cgcgccgctc aaggggaaag gttatttcgt ttttcacgat
     9481 gcttacggct atttcgaaaa acagttcgga ctgacaccgc ttggtcattt taccgttaac
     9541 cctgagattc aacctggcgc gcagcgttta catgaaataa gaacacagtt ggttgagcaa
     9601 aaagcaacct gcgtttttgc tgagccacag ttcaggccag cggtcgttga gagcgtcgca
     9661 cgagggacat ccgttcgtat gggaacgttg gatcccctgg ggacgaatat caaactgggt
     9721 aaaacaagct attcagaatt cctgagtcaa ttagccaacc agtatgcgag ctgcctgaaa
     9781 ggagattaat gaggaagtga ttacgtgcaa cagatagccc gctctgtcgc cttggcgttt
     9841 aataatttac cgcgaccaca ccgcgttatg ttggggtcgc tcaccgttct tactctggcc
     9901 gtcgctgtct ggcggcccta tgtttatcac cgtgatgcca cgccaattgt caaaaccatt
     9961 gagctggaac agaacgaaat tcgttcgctc ttacctgaag ccagtgagcc gattgatcaa
    10021 gctgcacaag aagatgaagc cattccccag gacgaactgg atgacaaaat cgccggtgaa
    10081 gcgggcgtgc atgaatatgt tgtttccact ggcgatacgc taagcagcat tctcaatcag
    10141 tatggtattg atatgggtga tatcacccaa cttgctgctg ccgacaaaga attgcgtaac
    10201 ctgaaaatcg gtcaacaact ctcctggaca ttaaccgcgg acggcgaact gcagcgcctc
    10261 acctgggaag tgtctcgtcg tgaaacccga acctatgacc gtactgccgc taacggtttt
    10321 aaaatgacca gcgaaatgca gcaaggagag tgggttaaca atctgctgaa aggtaccgtc
    10381 ggagggagct ttgttgccag cgccagaaac gccggtttaa ccagcgccga agtgagcgca
    10441 gtgattaaag ccatgcagtg gcaaatggat ttccgcaaac tgaaaaaagg cgatgaattt
    10501 gcggtattaa tgtctcgaga aatgcttgat ggtaaacgtg agcaaagcca gctgctgggc
    10561 gtacgtttgc gttcagaagg taaagattat tacgcaatcc gcgctgagga tggcaaattc
    10621 tacgatcgta acggtactgg tctggcgaaa ggattcttgc gattcccgac ggcgaaacag
    10681 ttccgtatct cgtctaactt taacccgcgt cgaactaatc cggtgacagg tcgcgttgca
    10741 ccacacagag gtgttgattt cgccatgcca caaggtacgc cagtgctttc agtgggtgac
    10801 ggtgaagtgg tggttgccaa acgcagtggc gcagcaggtt attatgtggc tattcgtcat
    10861 ggtcgcagct acaccacgcg ttatatgcac ttgcgcaaga ttctggtgaa accgggacag
    10921 aaggtgaaac gtggcgaccg tatcgcgctt tccggtaata ccggacgttc aactgggccg
    10981 catctgcact atgaagtatg gataaaccag caggccgtaa acccgctgac ggcaaaactg
    11041 ccgcgtaccg aagggctgac cggctccgat cgtcgcgaat tcctggcgca ggccaaagag
    11101 attgtgccgc agctacggtt tgattaatta acatccattc gcagccggta cgcagtcagt
    11161 accggctttt tttatttggt gcggggcaag ttgtgccgct acactatcac cagattgatt
    11221 tttgccttat ccgaaactgg aaaagcatgg aaacgaaaaa aaataatagc gaatacattc
    11281 ctgagtttga taaatccttt cgccacccgc gctactgggg agcatggctg ggtgtagcag
    11341 cgatggcggg tatcgcttta acgccgccaa agttccgtga tcccattctg gcacggctgg
    11401 gacgttttgc cggacgactg ggaaaaagct cacgccgtcg tgcgttaatc aatctgtcgc
    11461 tctgctttcc agaacgtagt gaagctgaac gcgaagcgat tgttgatgag atgtttgcca
    11521 ccgcgccgca agcgatggca atgatggctg agttggcaat acgcgggccg gagaaaattc
    11581 agccgcgcgt tggctggcaa gggctggaga tcatcgaaga gatgcggcgt aataacgaga
    11641 aagttatctt tctggtgccg cacggttggg ccgtcgatat tcctgccatg ctgatggcct
    11701 cgcaagggca gaaaatggca gcgatgttcc ataatcaggg caacccggtt tttgattatg
    11761 tctggaacac ggtgcgtcgt cgctttggcg gtcgtctgca tgcgagaaat gacggtatta
    11821 aaccattcat ccagtcggta cgtcaggggt actggggata ttatttaccc gatcaggatc
    11881 atggcccaga gcacagcgaa tttgtggatt tctttgccac ctataaagcg acgttgcccg
    11941 cgattggtcg tttgatgaaa gtatgccgtg cgcgcgttgt accgctgttt ccgatttatg
    12001 atggcaagac gcatcgtctg acgattcagg tgcgcccacc gatggatgat ctgttagagg
    12061 cggatgatca tacgattgag cggcggatga atgaagaagt cgagattttt gttggtccgc
    12121 gaccagaaca atacacctgg atactaaaat tgctgaaaac tcgcaaaccg ggcgaaatcc
    12181 agccgtataa gcgcaaagat ctttatccca tcaaataaaa aagcctctcg cgaggagagg
    12241 ccttcgcctg atgataagtt caagtttgct tcagaatatt cgaaatctgt tgaactatca
    12301 ttgaactata ggccggatgt ggcgttttcg ccgcatccgg caacgtactt actctaccgt
    12361 taaaatacgc gtggtattag tagaacccac ggtactcatc acgtcgcctt gggtgacaat
    12421 caccaggtca ccagacatca agtaatcttt atcgcgcagc agattaaccg cttcgctggc
    12481 agctgctacg ccgtcattag cgctatcaaa gtgcaccggc gtaacgccac gatagagagc
    12541 agtcaggttc agcgtacgtt catggcgcga catggcgaaa attggtagac cagagctgat
    12601 acgggaggtc atcagcgcgg tacgacccga ttcggtcatg gtaatgatcg ccgtaacgcc
    12661 cttcaggtgg ttagctgcgt acattgccga catggcaata gcttcttcca cattgtcgaa
    12721 ctgaacgtcc agacggtgtt tagaaacgtt gatgctcggg attttttccg cacccaggca
    12781 aacgcgcgcc atggctgcaa cagtttctga cggatactgc ccagcggcag tttctgcaga
    12841 cagcatcaca gcgtcagtac catccagaac ggcgtttgct acgtccatga cttctgcacg
    12901 cgtcggcatc gggttagtaa tcattgactc catcatctgg gtcgccgtga ttaccgctcg
    12961 gtttagctga cgcgcacgac ggatcaacgc tttctgaatg ccgaccagtt ccgggtcgcc
    13021 aatttccaca ccgaggtcgc cacgtgcaac cattaccacg tcagaggcga ggatgatgtc
    13081 atccattgca tcctggctgc aaacggcttc cgcacgttca accttggcaa caattttcgc
    13141 atcacatcct gcatcgcgtg ccaggcgacg ggcatagttc agatcttcgc cacagcgtgg
    13201 gaaggagaca gccaggtaat ctacgccaat caacgccgca gtcttaatgt ctgctttgtc
    13261 tttttcggtc agcgcttcag ccgacaaacc gccgccaagt ttgttgatac ctttattgtt
    13321 ggagagggga ccaccgacgg tgacttcggt gaacactttc atgccctgaa cttccagtac
    13381 ttttaactgg acgcgaccat cgtccagcag caggatgtca ccaggcacga cgtcagcagg
    13441 caggcctttg tagtcgatac cgactttttc tttgtcgcct tcacctttac ccaggttggc
    13501 gtcgagcagg aatttatccc caatattgag gaaaactttg ccttctttaa aggtggatac
    13561 acggattttg ggcccctgga ggtcacccag aatagccaca tgacgcccca gttttgcggc
    13621 aatctcacga actttatccg cgcgcatttt gtgatcttca ggcgagccgt gagaaaagtt
    13681 catacgtaca acgttggcac ccgccgcgat aactttttca agattattat cgcgatctgt
    13741 tgctgggcct aacgtggtaa cgatttttgt tctgcgaagc cttttggaca tgtaatactc
    13801 cgttgactga aacaaccagg tgttgcttga acatgaaatc cgaatgaaat aacgccgcga
    13861 tggcgataaa ttaatctcat tcggatgtcg tataagatta ggacagtgac agtcgctttt
    13921 agcgatcgtc acttaaatta agtaactgct tatcaaaacg cgattccttc agcgcttcct
    13981 tgacccgctt caagttatct ctgaattttg caccgcgtcg caaagtaaat cctgtcgcca
    14041 gcacatctat cacggtcagc tgtgcaagtc gagaaaccat gggcatataa atgtcagtat
    14101 cttccggtac gtcgagggta attgccagcg ttgcttcccg ggcgagcggg gtacccgcag
    14161 aggtgagggc aatcaccatg gcgtcgtttt cgcgtgccag ctgcgccagc tcgaccagat
    14221 tttttgttct tcctgtgtga gaaatcagca ccaccacgtc tccgtcgcta caattcatac
    14281 aactcatgcg ttgcagcacg atatcatcgg agtacaccac cggaacatta aaacgaaaga
    14341 acttattcat cgcatcgtgg gcaacggcgg cagaagagcc taatccgaaa aaggcgattt
    14401 tttttgcctg agtgagcaag tcgacggcgc ggttgatggc agatttatcc agtgaatgac
    14461 ggacatgatc gagcgttgcc attgcggact caaatatttt ccccgtgtat gattcaacgc
    14521 tgtcatcttc attgacgttg cgattaacat agggagtgcc attcgccaga ctttgtgcca
    14581 gatgaagttt aaaatcagga aaaccgcgcg tgtccatgct gcgacagaaa cgattcaccg
    14641 tcggttcgct aacattggct tccagcgcca gagcagcaat actcgaatgg atcgcgttat
    14701 cgggcgaagc cagaatgacc tcggcaactt tgcgctctga tttgctcaaa tgttccagct
    14761 gagactggat tttttccagc atattcatga tgtaaagaga ctcacgggta atgacgattt
    14821 ccgcactgaa agaaatcgaa atgcagtttt gtcagatatt acgcctgtgt gccgtgttaa
    14881 tgacaaaagc agataaaaaa gttgttattt ttttcataac atgatcagtg tcagattttt
    14941 accgaatgga aaacgatgat ttttttatca gttttgccgc actttgcgcg cttttcccgt
    15001 aatcgcacgg gtggataagc gtttacagtt ttcgcaagct cgtaaaagca gtacagtgca
    15061 ccgtaagaaa attacaagta taccctggct taagtaccgg gttagttaac ttaaggagaa
    15121 tgacatggcg gtaacgcaaa cagcccaggc ctgtgacctg gtcattttcg gcgcgaaagg
    15181 cgaccttgcg cgtcgtaaat tgctgccttc cctgtatcaa ctggaaaaag tcggtcagct
    15241 caacccggac acccggatta tcggcgtagg gcgtgctgac tgggataaag cggcatatac
    15301 caaagttgtc cgcgaggcgc tcgaaacttt catgaaagaa accattgatg aaggtttatg
    15361 ggacaccctg agtgcacgtc tggatttttg taatctcgat gtcaatgaca ctgctgcatt
    15421 caaccgtctc ggcgcgatgc tggatcaaaa aaatcgtatc actattaact actttgccat
    15481 gccgcccagc acttttggcg caatttgcaa agggcttggc gaggcaaaac tgaatgctaa
    15541 accggcacgc gtagtcatgg agaaaccgct ggggacgtcg ctggcgacct cgcaggaaat
    15601 caatgatcag gttggcgaat acttcgagga gtgccaggtt taccgtatcg accactatct
    15661 tggtaaagaa acggtgctga acctgttggc gctgcgtttt gctaactccc tgtttgtgaa
    15721 taactgggac aatcgcacca ttgatcatgt tgagattacc gtggcagaag aagtggggat
    15781 cgaagggcgc tggggctatt ttgataaagc cggtcagatg cgtgacatga ttcagaacca
    15841 cctgctacaa attctctgca tgattgcgat gtctccgcca tctgacctga gcgcagacag
    15901 catccgcgat gaaaaagtga aagtactgaa gtccctgcgc cgcatcgacc gctccaacgt
    15961 acgcgaaaaa accgtacgcg ggcaatatac tgcgggcttc gcccagggca aaaaagtgcc
    16021 gggatatctg gaagaagagg gcgcgaacaa aagcagcaat acagaaacct tcgtggcgat
    16081 ccgcgtcgac attgataact ggcgctgggc cggtgtgcca ttctacctgc gtactggtaa
    16141 acgtctgccg accaaatgtt ctgaagtcgt ggtctatttc aaaacacctg aactgaatct
    16201 gtttaaagag tcgtggcagg atctgccgca gaataaactg actatccgtc tgcaacctga
    16261 tgaaggcgtg gatatccagg tactgaataa agttcctggc cttgaccaca aacataacct
    16321 gcaaatcacc aagctggatc tgagctattc agaaaccttt aatcagacgc atctggcgga
    16381 tgcctatgaa cgtttgctgc tggaaaccat gcgtggtatt caggcactgt ttgtacgtcg
    16441 cgacgaagtg gaagaagcct ggaaatgggt agactccatt actgaggcgt gggcgatgga
    16501 caatgatgcg ccgaaaccgt atcaggccgg aacctgggga cccgttgcct cggtggcgat
    16561 gattacccgt gatggtcgtt cctggaatga gtttgagtaa tatcagcgct tatcctttat
    16621 ggttatttta ccggtaacat gatcttgcac agattgtaga acaattttta cactttcagg
    16681 cctcgtgcgg attcacccac gaggcttttt ttattacact gactgaaacg tttttgccct
    16741 atgagctccg gttacaggcg tttcactcat aaatcctctg aatgaaacgc gttgtgaatc
    16801 atcctgctct gacaactcaa tttcaggagc ctttatgaat ccacaattgt tacgcgtaac
    16861 aaatcgaatc attgaacgtt cgcgcgagac tcgctctgct tatctcgccc ggatagaaca
    16921 agcgaaaacg tcgaccgttc atcgttcgca gttggcatgc ggtaacctgg cacacggttt
    16981 cgctgcctgc cagccagaag acaaagcctc tttgaaaagc atgttgcgta acaatatcgc
    17041 catcatcacc tcctataacg acatgctctc cgcgcaccag ccttatgaac actatccaga
    17101 aatcattcgt aaagccctgc atgaagcgaa tgcggttggt caggttgcgg gcggtgttcc
    17161 ggtgatgtgt gatggtgtca cccaggggca ggatggaatg gaattgtcgc tgctaagccg
    17221 cgaagtgata gcgatgtctg cggcggtggg gctgtcccat aacatgtttg atggtgctct
    17281 gttcctcggt gtgtgcgaca agattgtccc gggtctgacg atggcagccc tgtcgtttgg
    17341 tcatttgcct gcggtgtttg tgccgtctgg accgatggca agcggtttgc caaataaaga
    17401 aaaagtgcgt attcgccagc tttatgccga aggtaaagtg gaccgcatg
//
LOCUS       DAEEDS010000081        17173 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEEDS010000081 DAEEDS010000000
VERSION     DAEEDS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.081"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..185
                     /locus_tag="KLH32_003055"
     CDS             <1..185
                     /locus_tag="KLH32_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBE0139797.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="KLH32_003056"
     CDS             complement(210..401)
                     /locus_tag="KLH32_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBE0139798.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="KLH32_003057"
     CDS             complement(506..742)
                     /locus_tag="KLH32_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBE0139799.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KLH32_003058"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KLH32_003058"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBE0139800.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KLH32_003059"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KLH32_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KLH32_003060"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KLH32_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBE0139801.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="KLH32_003061"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="KLH32_003061"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBE0139802.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="KLH32_003062"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="KLH32_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBE0139803.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="KLH32_003063"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="KLH32_003063"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBE0139804.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="KLH32_003064"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="KLH32_003064"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBE0139805.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="KLH32_003065"
     CDS             complement(10860..12233)
                     /locus_tag="KLH32_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139806.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="KLH32_003066"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="KLH32_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBE0139807.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="KLH32_003067"
     CDS             complement(13344..13583)
                     /locus_tag="KLH32_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139808.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="KLH32_003068"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="KLH32_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBE0139809.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="KLH32_003069"
     CDS             13960..14247
                     /locus_tag="KLH32_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBE0139810.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="KLH32_003070"
     CDS             14826..14969
                     /locus_tag="KLH32_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBE0139811.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="KLH32_003071"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="KLH32_003071"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE0139812.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="KLH32_003072"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="KLH32_003072"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBE0139813.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KLH32_003073"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KLH32_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBE0139814.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4156 c         4337 g         4148 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttc ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DAEEDS010000082        16386 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEEDS010000082 DAEEDS010000000
VERSION     DAEEDS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16386
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.082"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="KLH32_003074"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="KLH32_003074"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBE0139815.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KLH32_003075"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KLH32_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBE0139816.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="KLH32_003076"
     CDS             2337..2552
                     /locus_tag="KLH32_003076"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139817.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="KLH32_003077"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="KLH32_003077"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBE0139818.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="KLH32_003078"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="KLH32_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBE0139819.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="KLH32_003079"
     CDS             5918..6415
                     /locus_tag="KLH32_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBE0139820.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="KLH32_003080"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="KLH32_003080"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBE0139821.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="KLH32_003081"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="KLH32_003081"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBE0139822.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="KLH32_003082"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="KLH32_003082"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBE0139823.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="KLH32_003083"
     CDS             9100..9663
                     /locus_tag="KLH32_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBE0139824.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="KLH32_003084"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="KLH32_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBE0139825.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="KLH32_003085"
     CDS             complement(10255..11235)
                     /locus_tag="KLH32_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBE0139826.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="KLH32_003086"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="KLH32_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBE0139827.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="KLH32_003087"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="KLH32_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBE0139828.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="KLH32_003088"
     CDS             12538..12828
                     /locus_tag="KLH32_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBE0139829.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="KLH32_003089"
     CDS             12836..13429
                     /locus_tag="KLH32_003089"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBE0139830.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="KLH32_003090"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="KLH32_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBE0139831.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="KLH32_003091"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="KLH32_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16383
                     /locus_tag="KLH32_003092"
     CDS             15880..16383
                     /locus_tag="KLH32_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBE0139832.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4057 a         4220 c         4126 g         3983 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggaggccagt
    16381 taatgg
//
LOCUS       DAEEDS010000083        16011 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEEDS010000083 DAEEDS010000000
VERSION     DAEEDS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.083"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            44..375
                     /locus_tag="KLH32_003093"
                     /pseudo
     CDS             44..375
                     /locus_tag="KLH32_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            522..1883
                     /gene="yegQ"
                     /locus_tag="KLH32_003094"
     CDS             522..1883
                     /gene="yegQ"
                     /locus_tag="KLH32_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBE0139833.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2225..2542)
                     /locus_tag="KLH32_003095"
     CDS             complement(2225..2542)
                     /locus_tag="KLH32_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139834.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            2948..3847
                     /gene="yegS"
                     /locus_tag="KLH32_003096"
     CDS             2948..3847
                     /gene="yegS"
                     /locus_tag="KLH32_003096"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBE0139835.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(3929..4702)
                     /locus_tag="KLH32_003097"
     CDS             complement(3929..4702)
                     /locus_tag="KLH32_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0139836.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4808..5848)
                     /gene="gatD"
                     /locus_tag="KLH32_003098"
     CDS             complement(4808..5848)
                     /gene="gatD"
                     /locus_tag="KLH32_003098"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE0139837.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5896..7251)
                     /locus_tag="KLH32_003099"
     CDS             complement(5896..7251)
                     /locus_tag="KLH32_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBE0139838.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            complement(7255..7539)
                     /gene="gatB"
                     /locus_tag="KLH32_003100"
     CDS             complement(7255..7539)
                     /gene="gatB"
                     /locus_tag="KLH32_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBE0139839.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(7570..8022)
                     /gene="gatA"
                     /locus_tag="KLH32_003101"
     CDS             complement(7570..8022)
                     /gene="gatA"
                     /locus_tag="KLH32_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBE0139840.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(8032..9294)
                     /gene="gatZ"
                     /locus_tag="KLH32_003102"
     CDS             complement(8032..9294)
                     /gene="gatZ"
                     /locus_tag="KLH32_003102"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBE0139841.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            complement(9323..10177)
                     /gene="gatY"
                     /locus_tag="KLH32_003103"
     CDS             complement(9323..10177)
                     /gene="gatY"
                     /locus_tag="KLH32_003103"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBE0139842.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(10485..11537)
                     /gene="fbaB"
                     /locus_tag="KLH32_003104"
     CDS             complement(10485..11537)
                     /gene="fbaB"
                     /locus_tag="KLH32_003104"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBE0139843.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            11794..13071
                     /locus_tag="KLH32_003105"
     CDS             11794..13071
                     /locus_tag="KLH32_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139844.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13068..14071
                     /locus_tag="KLH32_003106"
                     /pseudo
     CDS             13068..14071
                     /locus_tag="KLH32_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            14068..15033
                     /locus_tag="KLH32_003107"
     CDS             14068..15033
                     /locus_tag="KLH32_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBE0139845.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15007..15753)
                     /locus_tag="KLH32_003108"
     CDS             complement(15007..15753)
                     /locus_tag="KLH32_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0139846.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(15801..>16011)
                     /locus_tag="KLH32_003109"
     CDS             complement(15801..>16011)
                     /locus_tag="KLH32_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBE0139847.1"
                     /translation="LAGYFNEYPWWVAHYYQRRPDNDGMAWRFWQHSDRGQVDGINGP
                     VDFNVFNGTGMSCRHSLMGLKKRLK"
BASE COUNT         4002 a         4132 c         3823 g         4054 t
ORIGIN      
        1 ttagatgctg ttttcgataa gcaatttcaa tcaaggagtc attatggctg gttggtttga
       61 actcagtaag agcagtgata atcagttccg gtttgtgcta aaagcgggca atggtgagac
      121 tatcctcacc agcgagctgt atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt
      181 gcgtagcaac agcccgcaag aagagcgcta tgagaaaaaa cggcaagcaa cggcaaattc
      241 tatttcaatc tgaaagccgc taatcatcaa attatcggct ccagccagat gtacgccacc
      301 gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag ccagacggtg
      361 aaagacaata cgtaattacg atgccgggcg tgggttgttg gcgtccggcg ctttaccaga
      421 tcccccctac agcgctacaa tgcccgccct taaagtgggg gcctccccta accgcttcat
      481 caggtgaagc ggatctgacc tgtcatcaga acgagagaat tatgtttaaa ccggaactcc
      541 tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc gcagatgctg
      601 tttatgcggg ccagccgcgt tactccctac gtgtgcgcaa caacgaattc aaccacgaaa
      661 atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat gtcgtggtca
      721 acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg
      781 aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg gtgcgtgagc
      841 acttccctga aatgccgatc cacctctcgg tgcaggctaa cgccgtgaac tgggcgacgg
      901 tgaaattctg gcagcaaatg ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg
      961 aagagattga agagatccat aatcaggtgc cggatatgga gatcgagatc ttcgttcacg
     1021 gcgcactgtg catggcctac tccggtcgct gcctgctctc tggctatatc aacaagcgcg
     1081 acccgaacca gggcacctgc accaacgcct gccgctggga gtacaacgtc caggaaggga
     1141 aagaagatga cgttggcaac atcgtacaca agtacgagcc gattccggtg caaaatgttg
     1201 agccgacgct gggtatcggc gcgccaaccg acaaagtgtt tatgatcgaa gaagcccagc
     1261 gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc atgaactcga
     1321 aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg cattcgctga
     1381 aaatcgaagg tcgtaccaaa tctttctact attgcgcacg caccgcacag gtttaccgca
     1441 aagctatcga tgacgccgct gcgggaaaac cgttcgatac cagcctgctg gaaactctgg
     1501 aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact cacgacgatt
     1561 atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt ggtgagttta
     1621 ccggtgagcg caagggggac ctcgcagcgg tagcggtgaa aaataaattc tccgttggcg
     1681 acagtcttga gctgatgacg ccgcaaggca acattaactt tacccttgag cacatggaaa
     1741 acgccaaagg cgaagctatg ccggtcgcac caggcgatgg ttatactgtg tggctcccgg
     1801 tcccgcagga tcttgagcta aattacgcgc tgctaatgcg taatttctcc ggggaaacca
     1861 cgcgtaaccc ccacggtaag tgattaattt cgattatttt tcccggatgg aaaattctta
     1921 gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct gaaaaacata
     1981 atccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat ctcccttaca
     2041 cgggcttatt ttttgtacat aagctgtaaa gaaggattga tccctgataa ccgtcttcca
     2101 ttgaccacat cgatagaatc ccccttcata gcacaatgcc tttcacgtaa cggtatcgtg
     2161 ctcgcccagg ttccggctac gcacagccag aacgcgcatg tttgacgctt acaaaaaaat
     2221 attctcactc tccacatttg aatgtcagac gagcgacgcc atgtaatcct gcaccttctg
     2281 tcttcaggtc aactatctgc atttttttgc cctgagtaac acagaaatgg gctgcatcat
     2341 tttttactat attttctgca ccagatattc tgcccctggc taaagaagct tcggcttcgg
     2401 tgtagtattg gttatcgagt ttacgctgaa tattactttt atatgcaaga ccaaatttac
     2461 cgatacttgt ttcatcatta tgcacagcac aaccagatat aagaaaaata ctaattaatg
     2521 atatagcagc tatctttttc atctcacctt cccccattaa ataccaacga cactctctag
     2581 tgtttaaata taataatggc atgattatta taattgaata ggattataat aaatgctctg
     2641 tacaacattt cctacataag taggaattac ggacattgag gcccttcagg gtaactccat
     2701 gggggcttta atatattata ttgaagatgc cactgtttag ttgaatatta ggtatatgct
     2761 cttttttgaa atttatcggt ggcagccgtt agtattcgct gtccccattg caagctcctg
     2821 gtggtaacca ctgaatcctc catacttgaa ctgacttttt atcctccgac tttcatcctg
     2881 ttctgactcc accttttgtt ttctgctcta cactatctac agaccaatca taaaggcaca
     2941 tacgatcatg gcagaatttc ccgccagctt actgattctt aatggtaaaa gtactgacaa
     3001 tctacccttg cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg
     3061 ggtcacctgg gagaaaggtg atgccgcacg atttgtagag gaggcccgga agttgggcgt
     3121 cgcaacggtg attgccggtg gtggcgatgg caccattaat gaagtttcta cggcgttgat
     3181 tcagtgtgag ggggatgaca tacccgcgct gggaattttg ccattaggaa ccgccaatga
     3241 ttttgccacc agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgc
     3301 cggtaatgcc attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat
     3361 ggcgacaggc ggatttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc
     3421 gctgggtggc gtctcttaca tcattcatgg cttaatgcgc atggatactc tgcaaccgga
     3481 ccgttgtgaa atccgcggtg aaaactttca ctggcaaggt gacgccctgg tcattggtat
     3541 tggtaacggg cgtcaggccg gtggcggtca acaattgtgc ccgaacgcgt taattaacga
     3601 tggcttgctg caactgcgca tttttaccgg cgatgaaatt cttccggctc tcgtatcaac
     3661 cttaaaatct gacgaagata acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat
     3721 acaagcccca cacgaaatca cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt
     3781 ccatattgaa atacttccgg cggcgttgcg ttgtcgatta ccaccagatt gtccattatt
     3841 gcgttaatca aactattctc tcttgaacca gggagaatcc gtaatgccgg atgcggtgta
     3901 aacaccttat ccggcataca gaacaatact acaccgtaat taattttacc cgtgtcttct
     3961 gcaatgccaa tttatccacc tctgacacag catcatcggt aacaataatg tcaatttttt
     4021 cgattggcag tacctgatta aaaccgcgac gattaaattt cgatgaatcg agaacagcaa
     4081 tcactttatt tgcggcggtg accatcgccc cgctaatcga ataaccttca ttaaaggtcg
     4141 taataccatt aaccgcatca ataccatcag caccgacaaa cattaaatcg gcattaatat
     4201 cctgcaatga acgctcggca atggaaccat gcatcgagcg cgttttatgg cggaccgtgc
     4261 caccacagac gaccagagta atgtctttat tttcggaaag ggcaaacgct gccgggagac
     4321 tgttggtaat caccgtgata tttttagcgg tcattaatcc ttcggcgata agcattgtgg
     4381 tacttccgct gtcgagaata acggtcatcc cttcatggat cattttgact gccgcctgcg
     4441 ctattctgtt ttttggcgcg ctggcgagct gaaatcgctc tttaaacccg acttcctggg
     4501 tctcggtttc actattaccg gacattattt tcgccgcacc gccatgaaag cgcgtaacca
     4561 cgcctttttg ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag gcagcaaata
     4621 ctcccgccag atcctgaaca agcacggttc cctgttcatt cactaattgg atgatcttat
     4681 tccttcgctc gaatgaattc atcgtcattt acccatattc aattgtggct agtgtaaacg
     4741 aagggggacc attggtgaat gatctgtttt gcacaatacc gggccagcat cacgctggcc
     4801 cgcggtttca tggaatgagc aacactttgc ccggcatggc attacgagcg atgtcacgca
     4861 ccgcctgggt gaagctttca aagcttccac ggtgagcgat taatggctcc aggcttaact
     4921 tacgttctgt cagcaaccgg ctcgccgttt cccactcctg ccccggccaa gggctggaat
     4981 agttcatcca actgccgata accgtcagct ctttacgcaa tattttgcca aacgttgccg
     5041 atgttaaatg cagatcctga tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg
     5101 caatctctac cgccagttcg acagtttgcg gtacgccagc cgtctcgagg ataagctgat
     5161 taaagcgcag ttcgcgtaaa acgctctgca tttgcggcgc gctcatttcg ctactgttaa
     5221 atgtttgcat cgcaccgaaa gattttgcca gtgccagttt ttctgagcta atgtcgatcg
     5281 ccgtcacact ctttgctccc agcgcgacag cgcactgaat agccagcagg ccaatggttc
     5341 cggcaccaat aataataacg tttttattct cacaaccttg cgctaaatga aaagcatgca
     5401 ggccaacggt aatcggctca ataaaagccc catcctcaat aggcatatcc gtgggtagag
     5461 caaagacatt ttttcgctta acgacaatat attcagcaaa tccaccatca cgccgcgagc
     5521 caataaaatc atattttgcg cactgggaat aaaacccttt caaacactct ggacaagtaa
     5581 aacagggtaa taacggcaca caggcaaccg catcgccagg gtgtaaatca tcaacaccgg
     5641 atcccaccgc atcaatatag ccgctaaatt catggcctaa cgttattgga taataatgtg
     5701 caccattttt aaatatcctg ggtaaatcgg aaccacataa gcccgagctg gcaattttta
     5761 cccgcacctc atcctgatgt ttaatttcag gaatgacgct ttttgcaacg cgcacgatac
     5821 catcagtatc attcactact gatttcataa aaactcctga ttgttaaggg ggataacctc
     5881 cccctcaggt aaaaattatt ctgcgagaac ggctttctct tgtttagtaa aaccacgcgc
     5941 tctacgccag gtcatgaaaa taccggtcag ataaattgcg ccgataataa tgaaaccggg
     6001 aatattttgc ggggagaaaa cctgaatcag taaccaggta atgggagaac cgccctgatc
     6061 cattgaagcc accatacccc cggcttttaa cgccccagca ttagccgccg gttgggtgtg
     6121 caaaccaata gtttgcgtcg cgatccacag ggtgatgctc ataatgatga cacccgagat
     6181 taaggtgcgg aacagatttc cacgatgcac cgcgaccgcc atcgccacga agaagccgat
     6241 agtggcaaga tcgccaaacg gcagtacctg attacccgga acacaaacag caattaaaat
     6301 ggtgagcgga ataaaaatca ggcttgccga taccaccgcc gtatgcccca gcagtaatgc
     6361 gggatccagg ccaatcagga actcctgacc gccgaacttc gcctgtaaac gactacgggc
     6421 ctgcttagcg atgggcgtta aaccatccat gatgggttta atcatccgtg gcatcagcag
     6481 cataactgcc gccgttttta ccgccagctg caatacacct ttgacatcgt aacccgcgag
     6541 gatgccgata atcagcccca tcacaaaacc cacggtgaca ggttcgccaa aagggccaaa
     6601 tttgcgctga atatcgtcgg cgctaaattt aattcggtta acgcctggga ttttctcgat
     6661 gatagcatcg accagcaccg caatcggccc catatacgcc gacgtaccgt gcggaatagc
     6721 aataccttcc agctcaaaga aatttcgggt atcgcgggca aaccagtcgc agagcttata
     6781 aacaaacgcc gcgtgaatta ccacacccgc catccctatc atccatgaac cggttgccag
     6841 gtgcagcaac gcgccggtga aggtcatatg ccagatattc cagatatcaa catttaccac
     6901 ccgcgtcata cgggtcagta acatcgccac gttaaccaat atcgcaatcg gaatcgccac
     6961 cagcgcaatt tgcgacgccc aggtcattgg tgaagagccc ggccagccga catcgaccac
     7021 atgcagattc aggtcgaaat tttccgccat cgctttcgcc gccggaccaa tggaatccag
     7081 cattaagcca atcacaaggc caatgccaac aaagccaatc ccgatatgca gacccgcttt
     7141 aaagcaatcg cctgccttca tgcctaatat tttagaaaaa ataatgatga caaccggcag
     7201 catcactgta gggccgaggt cgagaatata acgcatgact tctgaaaaca taggtcaccc
     7261 ctgtaagata gtcagaattt tattttgtaa tgcttcgata ccgacaccag aaacaaaagg
     7321 catgccgtga actaacggaa tatcgccaaa actacgatcc accctggcag tggtacatat
     7381 caaatgcaca ccatccatat aggtttctat ttcattaacc cgacactgga ttaattcaac
     7441 aggaatatta tgactctgac acaactcttt aatttcttcc gccgccatcg tagaggtcgc
     7501 aaccgcgcct ccgcaagcga caataatctt gcgtttcata aatagtactt tttattgtta
     7561 cagagggatt tattcgtctg aatctaaaac atactttgtg aagtattcct ttaactgagt
     7621 ttcaggaaga gtaattagtg tctcgacgat atcgggctgt tgtaacttgc caaataaaca
     7681 gcgtaaaagt ttcaattgct gctgcggatt ttccacaatc aacgcaataa ccaacgatac
     7741 cgccacgtcg ttatcatcat ccgcttgctg aaaatgaact ttatttgttg gccttaacag
     7801 ataaatggct gacgacttag catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg
     7861 ctcaagcatt atcccggtag ggaattctgc ttctctggca attaatgcct gtggccaggt
     7921 atcatgaacc acacctttcg cgagcatctc attaccgata taggttaaaa cttcgctacg
     7981 atcgacaaaa gaaattccgc tacgaacaaa caggttagtc atataccgtc cttattccgc
     8041 acagccgtag cgataggcgc gcaaaacatc ataaatttta ttcataataa gctgatgcgg
     8101 cattgctgat aattccccgg actgaatgcg ttcaaattgt ttgggaagat actgactaat
     8161 catgcccagt gggatgtcca cgccttgcag attcaccatc atcgtttcaa cgctattttt
     8221 aatccgacta tgcggccagt aataacgaat acgatccgac aggctgtaac gaatatccag
     8281 taatgaatcg ttaaaacccg tgcgataata ttttttccag tattgcggtt cgtcgagcat
     8341 cacttcttca attaccgcca ggcaaccgct gcgattttcg ggggcgataa gttcctgctc
     8401 aatttgtgcc agtgcaaata tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt
     8461 caatattgca aagtgatcgc ggactaattc ccaataagcc gtccgggtct ggtaatcggt
     8521 agaatgtgct tcataaacca ttcgggtgtt ttctatccat tgcgccagcg cctgcgcttc
     8581 ctgcggctga taatggataa tattgctgtg atcaaattcc acacccggct gcaccacgat
     8641 ggcaatcaca cgtgttaacg cctctgtcag cccacgggca ataaaggcct tttgatgcgt
     8701 acgtaaagta ttggcggcat cttcaacatg ggtgatgtgt actgactgaa tggcgctggc
     8761 ctcaccgccc ggaaccggaa cttcagtgcc aatgacatag ctcagttgct cacgctggca
     8821 atctgtcgcc acactttccg cagcaaagca aagcacagca gctcgttccg caaccgtttc
     8881 tggtgctaac ggtatggtat cccccgcgca ggacattgac gcatcaagat gaattttact
     8941 gaagccagca cgaacatatg cttttaccag ctcgacggat ttttccatcg ccgcatccgc
     9001 attttcttgc tgccagcagt ttggtcccag atgatcgccg ccgagaataa tgcgttcgcg
     9061 tgcaaaccca actttatcgg caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat
     9121 tccggtataa ccgccaaatt gattgacctg gtttgacgtt gcttcaatca gcactttgcg
     9181 cgtgctgttg cgatcaaatg ccagcgccgc ttcgataacc aacggatggg cagaacagac
     9241 tgaacatatg ccgatatgtt caccagcttt atgccgggca attaacgttt tcatgttttt
     9301 tccttgttaa atggcaggtg cgttatgccc tgccctcgca gccacaatcg gcaatcactt
     9361 tgctcaccac atcgcgcatt gcggatttag ccgactgcaa ataatcccgg ggatcggtcg
     9421 cttcggggtg ctcggtcagg taatttttta acgactgcaa gaaggcattt ttcagctccg
     9481 ttgcaacgtt gattttgcat atccccagtt tgatggtttg ctgaatatcc ttagtcgata
     9541 atcctgacgc gccatgcagc accagcggta agttcaccca ctggcgaatg ttctccagtc
     9601 gagaaaaatc aagcaccggt gcgctggcat acataccgtg agccgtgccg atggcgaccg
     9661 ccagggaatc aatcccggtt gcctcggcaa attcacgcgc ctgtgcgggg ttggtgtaca
     9721 acgcatcgac ttcattgact tgcacatcat cttcctggcc gccaagttgc cccagctccg
     9781 cctcgacgct gacatcaaag cgatggcaaa aatccaccac ctctttgacc cgtgaaatat
     9841 tttgcgcaaa aggcaaatgc gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct
     9901 tctgagcgat atcgtcaaat ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat
     9961 attgcttcgc catcgcgctg accagcgcca acagattttc tgtaccagca tgagtaaatg
    10021 tgccaggcgt tccggcgatg atgaccggcg catgcaggtt ggcagcggtt tccaccacca
    10081 cttgcatcgt ttcgagattg tgaatattga atgccggaac cgcataaccg ccgcgctgtg
    10141 cgttgttcag catctgcttt gtcgatacca cgtacatttt catatcttgt cgtttgcttt
    10201 cgatttcaaa atataatgaa attatttgct tttaaatgtc gagataatga tcacaaaaac
    10261 aacaatatga aaattgttcg aggagtgaaa ggcaaaaaaa cggcctcccg atagggaagc
    10321 cgtagcaaag tgcgcgtgtt tttatgccgg atgcggtgta aacatacgtg gcagtgctat
    10381 gtaggcatga taagacgctt ccgcgtcgca tcaggcatag gttgccggat gcagtgtaaa
    10441 cgtcttatcc gtcctacggg gtggtgctgt tcgtatgagg cgtatcaggc aatagtaatt
    10501 ttgctatcga gataaacgtc ctgcacggca ttaatcagtt tcacgccgtc agccatcgat
    10561 ttcttgaacg ccttacgtcc aagaatcagc cccattccgc ctgcgcgttt gttgataacc
    10621 gcagtacgca ctgcatcgct gaggtcagtt tcaccgcctg cagcaccgcc ggagtttatc
    10681 aacccagccc ggcccatata gcagttagct aactgataac gcaccagatc aatcgggttt
    10741 tcgctggtta acttgctgta cacgcgatcg tcggtataac cgtaattaat cgctttatag
    10801 ccgccgttat tttccgccat tttttgtttg acgatatctg cacctatggt tgccgccaga
    10861 tggttcgcct gaccagtcag gtcggcggaa acatggtaat caacgccatc tttcttaaag
    10921 gcggagtttc gcaaataggc ccacagcact gtcaccatgc ccagctcgtg cgcgcgttca
    10981 aaggccgcag aaatttcttc aatctggcga cgtgactctt ccgagccaaa atagatagtc
    11041 gcaccaaccg ccaccgcgcc catgttgaag gcctgctcca cgctggcata cagcgtttga
    11101 tcgtaggtat tcgggtaact tagcgtctcg ttgtgattaa gtttgacgag aaatggaatg
    11161 cgatgcgcat agcgccgcga taccgacacc aacacgccgt aagttgatgc cacacagtta
    11221 cagcccgctt cgatcgccag ttcaacaatg tttttcgggt caaagtagag cgggttagca
    11281 gcaaatgaag ctccggcaga gtgctcaacg ccctggtcaa ccggcagaat agaaagatat
    11341 cctgtgccag ccagacgccc ggtgttgtac aacgtctgca tattacgtaa caccgctggc
    11401 gggcgattat tgtcaatcat cacgcggtct acgtagtcat gtccggggag ataaagctgg
    11461 tcagaaggaa tagtcataca acggtgctgt aaaaggttgt cggcgtcttt gccaagcaac
    11521 tgcgcaatat ctgtcattac tatgctcccg taaattccga ttggatatcg gctatggatt
    11581 gtcctggccc gccttttgcg ggcaatcata atcctagtcg ttacgggcga gtttttccat
    11641 cttttggatg ttttttcagc gttttctgtt ggctcgattc atcagaaaaa atgttagcgc
    11701 ggtcaagttt tcaccgcaaa ggtattcaaa aggcattatt atgtggtatt gtcatcgcgt
    11761 accttacatt acctgtcatg aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc
    11821 atgatgtttg ttgaatggtt tatctggggc gcgtggtttg tgccattgtg gttgtggtta
    11881 agtaaaagcg gttttagtgc cggagaaatt ggctggtcgt atgcctgcac agccattgcg
    11941 gcgatcctgt cgccaattct ggttggctcc atcactgacc gctttttctc ggcgcagaaa
    12001 gtgctggcgg tattgatgtt cgctggtgct gtgctgatgt atttcgctgc gcaacagacc
    12061 acttttgccg ggttctttcc gttactgctg gcctactcgc taacctatat gccgaccatt
    12121 gcgctgacta acagcatcgc ttttgccaac gtgccggatg tggagcgtga tttcccacgc
    12181 attcgtgtga tgggcactat cggctggatt gcctccggtc tggcatgtgg tttcttgccg
    12241 caaatgctgg ggtatgccga tatctcaccg actaacatcc cgctgctgat taccgccgga
    12301 agttctgctc tgctcggtgt gtttgcgttt ttcctgcccg acacgccgcc aaaaagcacc
    12361 ggcaaaatgg acattaaagt catgctcggc ctggatgcgc tgatcctgct gcgcgataaa
    12421 aacttcctcg tctttttctt ctgttcattc ctgtttgcga tgccactggc gttctattac
    12481 atctttgcca acggttatct gaccgaagtt ggcatgaaaa acgccaccgg ctggatgacg
    12541 ctcggccagt tctctgaaat cttctttatg ctggcattgc cgtttttcac taaacgcttt
    12601 ggtatcaaaa aggtattatt gcttggtctg gtcaccgctg cgatctgcta tggcttcttt
    12661 atttacggta gtgcggatga atatttcacc tacgcgttac tgttcctcgg tattttgctt
    12721 cacggcgtaa gttacgattt ttactacgtt accgcttaca tctatgtcga taaaaaagcc
    12781 cccgtgcata tgcgtaccgc tgcgcagggg ctgatcacgc tctgctgcca gggcttcggc
    12841 agtttgctcg gctatcgtct tggcggtgtg atgatggaaa agatgttcgc ttatcaggaa
    12901 ccggtaaacg gactgacttt caactggtcc gggatgtgga ctttcggcgc ggtgatgatt
    12961 gccattatcg ccgtgctgtt catgattttt ttccgcgaat ccgacaacga aattacggct
    13021 atcaaggtcg atgatcgaga tattgcgttg acacaagggg aagttaaatg aaaacagaac
    13081 gtattctcgg tgctctttat gggcaggcgt taggggatgc gatggggatg ccctccgagc
    13141 tttggccacg cagccgcgtt aaagcacact ttggctggat tgaccgtttt ctgcctggac
    13201 caaaggagaa taacgcggcc tgttatttta accgcgccga attcaccgac gatacctcga
    13261 tggcctgtgt ctggcggatg cgttactgga atgtgaaggc aagatcgatc cgaatctgat
    13321 tgggcgtaat attctcgact gggcgttgcg tttcgacgcc tttaacaaaa acgtactagg
    13381 tccgacctcg aagattgcgc ttaacgccat tcgcgacggt aaacccgttg ctgaactgga
    13441 aaacaacggc gtaaccaacg gcgcagcgat gcgcgtctcg ccattaggtt gtttgcttcc
    13501 agcgcgtgat gttgattcct ttattgatga tgtggcgctg gcgtccagcc cgacccataa
    13561 atccgatctg gcggttgcag gcgcggtagt catcgcatgg gcgatttctc gtgccattga
    13621 cggagaacgc tggtcagcga ttgttgattc attgccttca attgcgcgac atgcacagca
    13681 aaaacgcacc accacgttca gcgcctcact ggcggcacgt ctggagattg cgctgaaaat
    13741 tgtgcgtaat gccgacggca ccgaatccgc cagcgaacag ctttaccagg tcgttggcgc
    13801 aggcaccagc actattgagt ccgttccgtg cgccattgcg ctagttgaac tggcacaaac
    13861 cgacccaaac cgctgcgccg tcctgtgcgc caatcttggc ggcgacacag acaccatagg
    13921 tgctatggcg acggcgattt gcggcgcgtt gcatggcatt aacgctgtcg atcctgcgtt
    13981 aaaggcggag ctggatgcgg taaatcagct tgatttcaac cgctatgcca cagcgctggc
    14041 gaaatatcgt caacaacggg aggcggtatg agcggcgctc gattacacac actgctgcct
    14101 gaattaacca cgcgtcagcc ggtgatggtc gtcggcgcgg cggtcattga cgtgatcgcc
    14161 gacgcttatg ccctcccctg gcgtgggtgc gatatcgaac tgaaacagca gagcgttaac
    14221 gttggcggct gcgcactgaa tattgccgtg gcgttaaagc gcctcggcat cgaagcgggt
    14281 aatgccttgc cgctcggtca gggcgtgtgg gcggagatta ttcgcaaccg gatggcgaaa
    14341 gaggggttaa tcagcctgat cgataacgcc gaaagcgata acggctggtg tctggcactg
    14401 gttgagccgg atggcgaacg cacttttatg tcattcagcg gtgtggaaaa tcaatggaat
    14461 cgccaatggt tggcacgatt aaccgttgcg cctggcaacc tgctctattt ttccggttat
    14521 caactggcct cgccctgcgg cgaattgtta gtggaatggc tggaaaagct gcaagacgtg
    14581 acgccgttta tcgatttttg cccacgtatt ggcgatatcc cggacgcatt actggcgcgg
    14641 atcatggcct gtcgaccttt agtgtcactc aatcgtcaag aggctgagat tgccgccgaa
    14701 cgttttgctt tatccgcaga gataacaaca cttggcaagc aatggcagga gaaatttgcc
    14761 gcgccgttga tcgttcgtct cgataaagaa ggcgcatggt atttcagcaa cgacgcttct
    14821 ggctgcattc cggcatttcc aacgcaagtt gtagacacca ttggggcggg cgacagtcat
    14881 gcaggtggcg tacttgccgg gctggcctct ggtctgccac tggcggatgc cgtattactg
    14941 ggcaatgcag tggcgtcgtg ggttgtcggg catcggggcg gtgattgtgc gccaacgcgc
    15001 gaggaactac tcctcgcaca caaaaacgta tagatcgctg cgacagtggc taatgctgta
    15061 ctcaataggc cgttgctgtt ggtcaagcgc cacttgcttg atcaccagca ccggtatttt
    15121 gctatctaac tgaatatgtg actgaaactc ggcatccggc atccgggcgc taacgcggga
    15181 acgcgtacgt tgtgggtaaa tgtgctggct gcggaaatag tcataaagag aaattccgat
    15241 ggcatcaaca tcatgaatta aatgcgccgg aacccacgat tcctcaatcg ataccgcttc
    15301 ttcatcgaca tagcgaatac gcttaagcaa aaagacatca cttcccgctt cgaccgccag
    15361 ctgctgcgca acttcttcgg gacatttcac gacacgctta ttaacccata gcgtatcggg
    15421 ctttttcccg cacaatacca cctgctgaga aaaacctctc gcttctttca acgagtattc
    15481 gaagatgttg ttgatctgcg tgccgtaacc gcgcgaacgc gtcaccacgc cctcttcttc
    15541 cagcgcctgc atcgccttgc gcaccgtaat gcgcgaaacg ccggttaact gacttaaatc
    15601 acgctcaccg ggcaaaatat tgccatgctc cagcaccccg ctgcgcacgg cattttttac
    15661 cgtttcggca aacttaatat aaagcggcgt gttatccgcc gctaaaatac gctcgttcag
    15721 ttgggcaatc agctgggtat gcgcttgttc catttgtctt tttcctgcca gggagtctgg
    15781 tgggagtata tcactgcttc ttatttaagg cgtttcttta atcccatcaa cgaatgcctg
    15841 caactcatcc ccgtgccatt aaacacatta aaatccaccg gaccattgat gccatctacc
    15901 tgtccacggt cggaatgctg ccagaagcgc caggccatgc cgtcattgtc cggacgacgt
    15961 tgataatagt gagccaccca ccacggatat tcattgaaat agcccgccag a
//
LOCUS       DAEEDS010000084        15408 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEEDS010000084 DAEEDS010000000
VERSION     DAEEDS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.084"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1173)
                     /locus_tag="KLH32_003110"
     CDS             complement(<1..1173)
                     /locus_tag="KLH32_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBE0139848.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="KLH32_003111"
     tRNA            complement(1372..1456)
                     /locus_tag="KLH32_003111"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="KLH32_003112"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="KLH32_003112"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBE0139849.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="KLH32_003113"
     CDS             2800..4302
                     /locus_tag="KLH32_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBE0139850.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KLH32_003114"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KLH32_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBE0139851.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KLH32_003115"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KLH32_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBE0139852.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="KLH32_003116"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="KLH32_003116"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBE0139853.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="KLH32_003117"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="KLH32_003117"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBE0139854.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="KLH32_003118"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="KLH32_003118"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBE0139855.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="KLH32_003119"
     CDS             complement(11867..13063)
                     /locus_tag="KLH32_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBE0139856.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="KLH32_003120"
     CDS             13256..13759
                     /locus_tag="KLH32_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0139857.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KLH32_003121"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KLH32_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBE0139858.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="KLH32_003122"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="KLH32_003122"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBE0139859.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3932 c         3966 g         3535 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcgtcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcacc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt ctggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatggcg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatctg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaagt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccata caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgaatttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgag
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DAEEDS010000085        15350 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEEDS010000085 DAEEDS010000000
VERSION     DAEEDS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.085"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..157
                     /locus_tag="KLH32_003123"
     CDS             <1..157
                     /locus_tag="KLH32_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0139860.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            386..976
                     /gene="uidR"
                     /locus_tag="KLH32_003124"
     CDS             386..976
                     /gene="uidR"
                     /locus_tag="KLH32_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBE0139861.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1364..3037
                     /gene="uidA"
                     /locus_tag="KLH32_003125"
     CDS             1364..3037
                     /gene="uidA"
                     /locus_tag="KLH32_003125"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBE0139862.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3073..4098
                     /gene="uidB"
                     /locus_tag="KLH32_003126"
                     /pseudo
     CDS             3073..4098
                     /gene="uidB"
                     /locus_tag="KLH32_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4137..5402
                     /gene="uidC"
                     /locus_tag="KLH32_003127"
     CDS             4137..5402
                     /gene="uidC"
                     /locus_tag="KLH32_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBE0139863.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5628..7136)
                     /locus_tag="KLH32_003128"
     CDS             complement(5628..7136)
                     /locus_tag="KLH32_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBE0139864.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KLH32_003129"
     CDS             complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KLH32_003129"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBE0139865.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8611..10257
                     /gene="fumB"
                     /locus_tag="KLH32_003130"
     CDS             8611..10257
                     /gene="fumB"
                     /locus_tag="KLH32_003130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE0139866.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10400..11803
                     /gene="fumC"
                     /locus_tag="KLH32_003131"
     CDS             10400..11803
                     /gene="fumC"
                     /locus_tag="KLH32_003131"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBE0139867.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KLH32_003132"
     CDS             complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KLH32_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBE0139868.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPTTLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KLH32_003133"
     CDS             complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KLH32_003133"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBE0139869.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14285..>15350)
                     /locus_tag="KLH32_003134"
     CDS             complement(14285..>15350)
                     /locus_tag="KLH32_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBE0139870.1"
                     /translation="SAGIGKMSLTIVACMLCLVMIAGLVGLAIASAIGLDASAFGHMQ
                     SSLTSDDVSKTAAVSLPRLITSLIPTNLFLDLTGARSVSVIGIVIFTILAGIALLKVK
                     KDAPAEGEKLSAGINAIQIWVMKMVRIVIALTPYGVMALMAKVFSTYQLSQFTNLLGF
                     IVACYLAVFVMFIVHSGILLLIGKNPVNYYKTVWQVLTFAFVSRSSAASIPLAISALE
                     KLGVPNTIANIAASFGSSMGQNGCAGIYPAVMVAMIAPTMGIDPFSLQFLATLLPAIA
                     LGSIGVAGVGSGGTFAALIVLSTLNFPVALVVIFIAIEPIVDMARTALNVNGSIMSGV
                     ISARLLKDRETKEQQSVTLN"
BASE COUNT         3933 a         3634 c         3930 g         3853 t
ORIGIN      
        1 aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac cgcaagatat
       61 tgctaacgcg gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg gacaaattct
      121 caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac cggtaaacgg
      181 tcgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg ctaaacaact
      241 ggcagccaaa tcacggctat tggttaacca attgctgggt gaaaagtata cgaatagagt
      301 gtaccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg gtaaactagt
      361 tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag cacaaccgac
      421 acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat ttcacagtgc
      481 ctcgatgaaa gccatctgta aatcttgcgc cattagtcct gggacgctct atcaccattt
      541 catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga gggcgctggc
      601 tcgtttccgg gaaccgattg aagggattca ttttgttgac tatatggtcg agtctattgt
      661 ctctctcacc catgaagcct ttgggcaacg ggcgctggtg gtcgaaatta tggcggaagg
      721 gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga tcactgaatt
      781 tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag acatcaacac
      841 ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg atatcgaagc
      901 ggaagacctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga ttggcggtat
      961 attaaccgca tcctgatcct ctcatttcgc gggctggtga taactgtgcc cgcgtttcat
     1021 atcgtaattt ctctgtgcaa aaattatcct ccccgtgttc ggagaattcc cccccaaaat
     1081 attcaccgta gccatatgtc atgagagttt atcgttccca atactctcga acgaacgttc
     1141 gattgcttat tttatggctt ctgtcaacgc tgttttaaag attaatgcga tccatatcac
     1201 gctgtgggta ttgcagtttt tggttttttg atcgcggtgt cagttctttt tatttccatt
     1261 tctcttccat gggtttctca cagataactg tgtgcaacac agaattggtt aactaatcag
     1321 attaaaggtt gaccagtatt tttatcttaa tgaggagtcc cttatgttac gtcctgtaga
     1381 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
     1441 aaactgtgga attgatcagc gttggtgcga aagcgcgtta caagaaagcc gggcaattgc
     1501 agtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt atgtgggcaa
     1561 cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct
     1621 gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga
     1681 gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa
     1741 aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg
     1801 aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa
     1861 ctatgccggg atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga
     1921 tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt
     1981 ggtggccaat ggtgatatca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac
     2041 tggacaaggc accagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga
     2101 aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc
     2161 gctgcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa
     2221 accgttctac tttactggct ttggtcgtca tgaagatgcg gatttgcgag gcaaaggatt
     2281 cgataacgtg ctgatggtgc acgatcacgc attaatggac tggattgggg ccaactccta
     2341 ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat
     2401 cgtggtgatt gatgaaactg cagctgtcgg ctttaacctc tctttaggca ttggtttcga
     2461 agcgggcaac aagccgaaag aactgtacag cgaagaggct gtcaacgggg aaactcagca
     2521 ggcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt
     2581 gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc
     2641 gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt
     2701 aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa
     2761 ccgttattac ggttggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga
     2821 aaaagaactg ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg
     2881 cgtggatacg ttagccggcc tgcactcaat gtacaccgac atgtggagtg aagagtatca
     2941 gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga
     3001 acaggtatgg aatttcgccg attttgcgac ctcgtaaggc atattgcgcg ttggcggtaa
     3061 caattgacct acatgggcct cgggctttgc tacagcctgg tgaatattcc ttatggttca
     3121 cttgctaccg cgatgaccca acaaccacaa tctcgcgccc gtctgggcgc ggctcgtggg
     3181 attgccgctt cattgacctt tgtatgcctg gcatttctga taggaccgag cattaagaac
     3241 tccagcccgg aagagatggt gtcggtatac catttctgga cgattgtgct ggtgattgcc
     3301 ggaatggtgc tttacttcat ctgcttcaaa tcgacgcgtg aaaatgtggt acgtatcgtg
     3361 gcgcagccgt cattgaagat cagtttgcaa accctgaaac ggaatcgccc gctgtttatg
     3421 ttgtgcatcg gtgcgctgtg tgtgctgatt tcgaccttcg cggtcagcgc ctcgtcgttg
     3481 ttctacgtgc gctatgtgtt aaatgatacc gggctgttca ctgtgctggt actggtgcaa
     3541 aacctggttg gtactgtggc atcggcaccg cttgtgccgg ggatggtcgc gaggatcggt
     3601 aaaaagaata ccttcctgat tggcgctttg ctgggaacct gcggttatct gctattcttc
     3661 tgggtttccg tctggtcact gccggtggcg ttggttgcgt tggccatcgc ttcaattggt
     3721 cagggcgtta ctatgactgt gatgtgggca ctggaagctg ataccgtaga atacggtgaa
     3781 tacctgaccg gcgtgcgaat tgaagggctc acctattcac tattctcatt tacccgtaaa
     3841 tgcggtcagg caatcggagg ttcaattcct gcctttattt tgggattaag cggatatatc
     3901 gccaatcagg tgtaaacgcc ggaagttatt atgggcatcc gcacatcaat tgccttagta
     3961 ccttgcggat ttatgctact ggcattcgtt attatctggt tttatccgct cacggataaa
     4021 aaattcaaag aaatcgtggt tgaaattgat aatcgtaaaa aagtgcagca gcaattaatc
     4081 agcgatatca ctaattaata ttcaataaaa ataatcagaa catcaaaggt gcaactatga
     4141 gaaaaatagt ggccatggcc gttatttgcc tgacggctgc ctctggcctt acctctgctt
     4201 acgcggcgca actggctgac gatgaagcgg gactacgcat cagactgaaa aacgaattgc
     4261 gcagggctga taagcccagt gctggcgcgg gaagagatat ttacgcatgg gtacagggag
     4321 gattgctcga tttcaatagt ggttattatt ccaatattgt tggcgttgaa ggcggagcgt
     4381 attatgttta taaattaggt gctcgtgctg atatgagtac ccggtggtat cttgatggtg
     4441 ataaaagttt tggctttgcc ctgggggcag taaaaataaa acccagtgaa aatagcctgc
     4501 ttaaattagg tcgcttcggg acggattata gttatggtag cttaccttat cgtattccgt
     4561 taatggttgg cagttcgcaa cgtacattac cgacagtttc tgaaggagca ttaggttatt
     4621 gggctttaac gccaaatatt gatctgtggg gaatgtggcg ttcacgagta tttttatgga
     4681 ctgattcaac aaccggtatt cgtgatgaag gggtgtataa cagccagacg ggaaaatacg
     4741 ataaacatcg cgcacgttct tttttagccg ccagttggca tgatgatacc agtcgctatt
     4801 ctctgggggc atcggtacag aaagatgttt ccaatcagat acaaagtatt ctcgagaaaa
     4861 gcataccgct cgacccgaat tatacgttga aaggggagtt gctcggcttt tacgcgcagc
     4921 tcgaaggttt aagtcgtaat accagccagc ccaatgaaac ggcgttggtt agtggacaat
     4981 tgacctggaa tgcgccgtgg ggaagcgtat ttggcagtgg tggttatttg cgccatgcaa
     5041 tgaatggtgc cgtggtggat accgacattg gctatccctt ttcattaagt cttgatcgta
     5101 accgtgaagg aatgcagtcc tggcaattag gtgccaacta ccgtgtaact ccgcaattta
     5161 cgctgacatt tgcaccgatt gtgactcgcg gctatgaatc cagtaaacga gatgtgcgga
     5221 ttgaaggcgc gggtatctta ggtggtatga actatcgggt cagcgaaggg ccgttacaag
     5281 ggatgaattt ctttcttgct gccgataaag ggcgggaaaa gcgcgatggc agtacgctgg
     5341 gcgatcgcct gaattactgg gatgtgaaaa tgagtattca gtatgacttt atgctgaagt
     5401 aagaaataac gccggagaga aaaacctccg gcgtttcaga ttgttgacaa agtgcgcgtt
     5461 ttttatgccg gatgcggcgt aaacgcctta tccagcctac aaaaactcat aaattcaaag
     5521 tgttgcagga aaaggtaggc ctgataagcg tagcgcatca ggcaatctct ggtttgtttt
     5581 cagatgaaaa cgccggagtg aaaattctcc ggcgttttgg ccgtgaatta ctgctgcgga
     5641 attgccggta cagccggaac gttaagagct ggcatcgcaa acatgccaac aaaatcttct
     5701 aacgacattt tctgcccatt taacgttatc tgaccgttag catattgcag gctggtggtg
     5761 atggtattgt cctgcaaggt ggtcagacgg aacatctgcc ccattgctga tgcaccttca
     5821 acttgctgtt tcgccagttt tttcgcttga tcttcctgat aaccttccag cttcgctacc
     5881 tgagtcatca actcagttgc catatccacc ggaatggtca gtttcgcatc cagagattta
     5941 accgaacgat ctacttcctg cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct
     6001 ttcaggaaca gcgacagatt cagcgcactt tcaccctgac tgtttttcca gcttagcggc
     6061 gcgatagtaa tcaccggatc gcctttcaac atcagcggca gggcgctaaa gaaggcttcc
     6121 gtcactttct cctgataaag ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc
     6181 tgagtttgcg cgttatattg ctggctaaac tgatgccagg cttcaccatc aatctggccg
     6241 acttttaaag tcagcttgcc gctgcccaga tcctgattct gtaccttcag gctgtttagc
     6301 gagtaatcca gttggctatt gatcgtttta ccgtcattga ccagatccga tttaccgctg
     6361 atctccatgc cttccagcag tgccagttct ttgccttcca ctgaaatggt cattttttcc
     6421 agtgacagtt tttggtttcc tacgcgctca ccaaaacttc ccagcgtgct ggaaccgtcg
     6481 gttttcagat tattaaaggt caactgcact ttctggttgt attcgttaac tgcgtctatc
     6541 cgaccacttt gcgcctctcc ggaaagggag atggctttgc cgtctctgtc agcatttaac
     6601 tggaactcgc cgccgctaaa ggcgactttt tcatcctttt gctcgtaatt cagtggattg
     6661 agcgaaatat cggaactgga atcaccgctg taaccaatgc gcgagttaat ctcaaaaggc
     6721 gtttcacctt ttgccatatc aaacagtggt ttgcttactt cgttattaac cagcgtggtt
     6781 tgaattgatg ccatcgacgg gatcaggttc agttttttaa gctgggcaag cgggaaggga
     6841 ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc ttttaatcca cggattttct
     6901 ttcccggcaa tgggtttcac caacagttgc aactggctgc tgaatacgcc gcgatgatag
     6961 ttttgataac tcacttccag gttggattca ggagctgtca gtttgagttg cgcgttcgcc
     7021 tgcgcgacca tgtcttcgag atgggtttca atcttcttgc ctgtatacca tgcgccgcct
     7081 gtccagacta cgcctagcgc aacaatgacg cctaccgcta ccagcgattt attcataatg
     7141 attatccata aaatgaaatc aggcggactg gccgcctgaa ggtgttataa gcctttaata
     7201 agcttagcaa gagatgttaa ttttttcagt aagctcttac agcttgttgt aaacacgcgc
     7261 taaacggccg tggcctttga cagtcaccgg tgattcgttg gcggcaataa acgctgattc
     7321 acccggttta agctgtaact gctgagaacc tttccacaac gttgcatcgc cttcgacgca
     7381 gaacaaaatg gcggcactct gctggctgat ggtggtttct ttatcactaa ggtcatgcag
     7441 cgagaaggca aaatcatcca caggaatcgg gaagtccagt tctgcacctt gtttcaccgg
     7501 ctgggtcaac aactggttag ccggtttggc ttcgaatttc acattggcaa ccagttccgg
     7561 aatatcaatg tatttaggcg tcagacctgc acgcagcacg ttatcggagt tcgccatcac
     7621 ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc
     7681 gccaggattc aatttcacca cattcagtaa cagcggggag aacaggccgc tgtcttccgg
     7741 gtaaaattca gaaattaaac gaatcgtttg ccacggttca ccctgctgac tgtcgagcgc
     7801 cgattttaaa atcgccagcg cgcgggattt ttcttcaccc tgcatattca acaggttggc
     7861 gaacagttcg cttaaacgtt cggcatgtgg ctgttgtaaa aagtgagcaa tcgccggatg
     7921 tgcaccggcg accggctgga gtagggagac aatctcggaa aattcacgaa acgcgttcat
     7981 cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg tggttaggat ctttatagtt
     8041 acgctcggcg gcatccatcg ggatacctgc ggcattttct ttggcaaaac cgatttcaga
     8101 attgtgtttg tttggatgaa cctgaatgga gagtggctgt gctgcgcata atactttgaa
     8161 caggaaaggc agttcgccaa agcgtttggc aacggcctct ccgagcagag tcgatttatc
     8221 actctcaatc acatcacgca gtgaaacgat atctccggcg gcattctgca ctcgtgaact
     8281 gcttttcgga tgtgcgccca tccacagctc ggccatcggc tggctggacg gattttccat
     8341 accataaagt tcagtcaacg ccgttttgct accccaggca tagttttgca ctgagttaat
     8401 gagtttttgc atgatcaatc cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag
     8461 aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa
     8521 aaactgtgta ggatattgtt actcgctttt aacagggcaa cggaacaccc gcccagagca
     8581 taaccaaacc aggcagtaag tgagagaaca atgtcaaaca aaccctttca ttatcagtct
     8641 ccttttccac tcaaaaaaaa tgatactgag tattacctgc taaccagtga acacgttagc
     8701 gtatctgaat ttgaagggca ggagattttg aaagtcgcac ccgaagcgtt aactctgttg
     8761 gcgcgtcagg cgtttcatga tgcgtcgttt atgctgcgtc cggctcacca acaacaggtg
     8821 gccgacattc tgcgtgaccc ggaggccagc gaaaatgata aatatgtggc gctgcaattc
     8881 ctgcgtaact ccgacatcgc ggcgaaaggc gttctgccaa cctgtcagga taccggcacc
     8941 gcgattattg ttggtaaaaa agggcagcgt gtatggaccg gtggtggtga tgaagcggcg
     9001 ctggcgcgcg gtgtctataa cacttatatc gaagataatc tgcgctactc gcaaaacgcg
     9061 ccgctggata tgtataaaga agtgaatacc ggcaccaatc tgccagcgca gatcgatctt
     9121 tatgccgttg atggcgacga atacaaattc ctctgtatcg caaaaggtgg tggttcggca
     9181 aacaagacgt atctctatca ggaaaccaaa gcgttactga cgccggggaa actgaaaaat
     9241 tacctggttg agaagatgcg cacgctgggt accgcggcct gtcctccgta tcatattgcg
     9301 ttcgttattg gtggaacttc tgcagaaacg aaccttaaaa cggtgaaact ggcttccgcg
     9361 aaatactatg atgaactgcc aacggaaggg aatgagcacg gtcaggcgtt ccgcgatgtg
     9421 gaactggaaa aagaattact gatcgaagcg caaaatctcg gtctgggtgc gcagtttggt
     9481 ggtaaatact tcgctcacga catccgcgtg attcgcctgc cacgtcacgg cgcatcctgt
     9541 ccggttggta tgggtgtttc ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt
     9601 caggggatct ggatcgaaaa actggaacat aatccaggta aatatatccc tgaagagctg
     9661 cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta accgtccgat gaaagagatc
     9721 ctcgcacagt tgtcgcagta tcccgtttct acacgcttat cgcttaacgg cacgattatc
     9781 gttggtcgtg atattgctca cgccaaactg aaagagcgga tggataacgg tgaagggctg
     9841 ccgcaataca ttaaagatca tccgatttac tacgcgggtc cggccaaaac gccggaaggt
     9901 tatgcctccg gttctcttgg cccaacgacc gccggacgga tggattctta tgtcgatcaa
     9961 ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg
    10021 acggatgcct gtaaaaaaca cggcggcttc taccttggca gtatcggtgg tccggccgct
    10081 gtattggcgc agggaagtat taagagcctg gaatgtgttg aatatccgga actgggaatg
    10141 gaagccatct ggaaaattga agtggaagat ttcccggcgt ttatccttgt ggatgataaa
    10201 ggaaatgact tcttccagca gatacaactc acacagtgca ctcgctgtgt gaaataaaca
    10261 gagccgccct tcggggcggt ttttttacat ggcacgaaag accaaacatt tgttatcaaa
    10321 tagtaaataa tgagtgagct aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta
    10381 atcaggtgag gagcaggcca tgaatacagt acgcagcgaa aaagattcga tgggggcgat
    10441 tgatgtcccg gcagataagc tgtggggcgc acaaactcaa cgctcgctgg agcatttccg
    10501 catttcgacg gagaaaatgc ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc
    10561 agcggcaaaa gttaatgaag atttagactt gttgtctgaa gagaaagcga gcgccattcg
    10621 gcaggcggcg gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg
    10681 gcagaccggc tccggcacgc aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc
    10741 cagtgaatta ctcggcggtg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt
    10801 gaacaaaagc caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcgctgct
    10861 ggcactgcgc aagcaactca ttccgcagct taaaaccctg acacagacgc tgagtgaaaa
    10921 atcgcgtgca tttgccgata tcgtcaaaat cggtcgaacc cacttgcagg acgccacgcc
    10981 gctaacacta gggcaggaga tttccggctg ggtagcgatg ctcgagcata atctcaaaca
    11041 tatcgaatac agcctgcctc acgtagcgga actggctctt ggcggtacag cggtgggtac
    11101 tggactaaat acccatccgg agtatgcgcg tcgcgtagca gatgaactgg cagtcattac
    11161 ctgtgcaccg tttgttaccg cgccgaacaa atttgaagcg cttgcgacct gtgatgccct
    11221 ggttcaggcg cacggcgcgt tgaaagggtt ggctgcgtca ctgatgaaaa tcgccaatga
    11281 tgtccgctgg ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa
    11341 tgagccgggc agctcaatca tgccggggaa agtgaaccca acccagtgtg aagcattaac
    11401 catgctctgc tgtcaggtaa tggggaacga cgtggcgatc aacatgggtg gcgcttccgg
    11461 taactttgaa ctgaacgtct tccgtccgat ggtgatccat aatttcctgc aatcggtgcg
    11521 cttgctggca gatggcatgg aaagttttaa caaacactgc gcagtgggta ttgaaccgaa
    11581 tcgtgagcga atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac
    11641 ccacattggt tatgacaaag ccgcggagat cgccaaaaaa gcgcataaag aagggctgac
    11701 cttaaaagct gcggcccttg cgctggggta tcttagcgaa gccgagtttg acagctgggt
    11761 acggccagaa cagatggtcg gcagtatgaa agccgggcgt taatctgcaa catacaggtg
    11821 cagccgtgga atgatcaaac gaagcggctg cgcctgaggt ttataacggt gctgcacatt
    11881 gtcggcatcg taatttaaca actcaccaac gtccggcacg cccgcgccat tatcccgatt
    11941 aatcagtgca atcagtggtg tagggcaggc gtgttggact tgtttttgat ctcctttgta
    12001 ccagacgcgg gcaatcggct gcaccttcac tggacgtttg atttttaact tcgcgttctg
    12061 tggcagggca gcgatatcct gatactctcg ctccagtttt ctttgccact cctcgcgcgt
    12121 ccacggtgcg acactgcgtg gtgatttcag gcttttttcc agctgtgcca ggacttcatc
    12181 acgatgcaaa ttcttaatga tatgtttatt agcccaacca aagcgtaaag tggtggggtc
    12241 gtgcagaacg gtgagcgtgc ggtaagcatt aagggtgatc agccccggca aatgacgatg
    12301 cacccattca aaacgtgccg cggtggggag ttctgattca accgtgacga tatgctcgaa
    12361 cgtggtcttg agtttattga tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt
    12421 atcgacctgg taacacaaca cgccaggcag acggacagcg gccttgctgc tgcgattttc
    12481 ggactgttgt tgaataaata aatggcggaa atgacgcaac gccagcgact gcgcgtcgtt
    12541 gccgagatgt tgttttacct caatacgatt aagcggatta tgctcatcct ctttttttac
    12601 ctccggcaaa gagaacacgc gggcaaccaa tagcttgtgt tgctcaagat gagcggcaaa
    12661 tgtagccagc tcttgttcca tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta
    12721 acgcgccata atattaacca ctttagttac aacatactta ttttatagca cagtcgtggt
    12781 ggcttgacca tcgtgcgcgt agtgtcaggc agaggtaaat tgcgggatgt tatgccataa
    12841 cggccagcta aacgagaagc gagcaccacc cagttcgctg atgtcacaat taaccgtacc
    12901 gcccattgcc agtgctattg agtggacaat tgccagcccc agcccgcagc cgccggttga
    12961 gcgatcccgg ctgggatcga ggcgaacaaa aggttcaaag atatgttcgc ggttttctgg
    13021 ggcaatccct gggccatcat cctcaacaat taatgtcgct ctattccccg acagtagcag
    13081 gctggtttca accgttgaat ggcagtagcg cagggcgtta ttgagcaaat tatccagcac
    13141 gcgctccatt aagcgcatat ccaacgccgc ataatggcct tgcatgagcg tttttatccg
    13201 taccgtttta tcgggcgtta ctgcctgaat atctgccaga tgcgttgaca gccacaacgg
    13261 caggtctggt tcgctaagat gaagctcgtt ttgtgggcga tcgagtcggg cataagtcag
    13321 cagctcttca attaaagctt caagttgact gatatcacga ttcaacgcct gggattcggc
    13381 ggcgctcagg ttatcgctca tctccagtcg ataacgcagg cgcactaacg gtgttcgcag
    13441 ttcgtgagcg ataccgtcaa taagctgttt tttgctggca attaaggcgt tgatattgtc
    13501 cgccatctgg ttaaatgcga taccaagtcg ttcaaagctc gaaccctcat caaagtggat
    13561 acgttcattg agatgcccat cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc
    13621 ctgccagtgc ggacgcatcc agataaacac cggaaaggcg agggaaatag caataaaagc
    13681 gatcagggcg atatccagca atcgcatttg atggaggtaa taaagataag gaacaggacc
    13741 aactgccagc acgtagtggc tgcgcgggat acgctgcaaa aacgtgtact gatcgtccag
    13801 ggcgacaatt tcgccgccac gcagtcggtg catggaaata tcatcaagat ggtatttact
    13861 cagtggctcg acacgcagat cgaaagagag atttaaatcc atctctttca gcgtcttacc
    13921 ccagtcgtgt ggggggatct ctcgcaattc gctgcgcatc agatacagcg aactgttcat
    13981 caaatcatcc agcgactgtt tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac
    14041 cagcagagac atcacaagga agcagacaaa caataacagg taaaactgga taaacaattt
    14101 tttcattggc atcgcccgca acagaatact attcgtatta attcacgact gtggagcaat
    14161 gtaaaccgcc aactggataa gaccttgttt ggaagataat gtccggaaca gacggatgta
    14221 tccccggtgt attcatattc agtaaattca attttacacc ggggcttgtt tattatgcta
    14281 aggtttaatt taacgtgacg ctttgttgtt ctttagtctc tctatcctta agaagacggg
    14341 ctgatattac accggacatt attgaaccat tcacattaag cgctgttctt gccatatcaa
    14401 cgatcggctc tatggcaata aaaataacta ctaatgcgac agggaaatta agtgttgaaa
    14461 ggacaatcag cgccgcgaat gtcccaccac tacctacgcc tgctacccct attgagccaa
    14521 gcgcgatggc aggtaaaaga gttgcgagga attgcaatga gaagggatcg atacccattg
    14581 ttggagcaat catcgccacc atcacggcag gataaatccc agcgcagcca ttttgtccca
    14641 tgctggaacc aaaagacgct gcaatattag caatcgtatt tggaaccccc aatttttcca
    14701 atgcagaaat agccagagga attgaagccg cactactgcg agaaacaaac gcaaaggtaa
    14761 gtacttgcca gacggtcttg taatagttaa cggggttttt acctattaac agcaaaatgc
    14821 ccgagtgaac aataaacatc acaaaaacgg ccagataaca ggcgacgatg aacccgagca
    14881 aattggtaaa ctgtgaaagc tgatacgttg agaagacttt cgccattagt gccattaccc
    14941 cataaggcgt cagcgcaatg acaatacgga ccattttcat tacccatatt tgaatcgcgt
    15001 tgatccccgc gctcaatttt tcgccctctg ctggtgcatc tttctttact tttaacagcg
    15061 caataccagc aagaattgta aagatgacga tgccaataac cgaaacactt cttgccccgg
    15121 tcagatcaag gaagaggttg gttgggatca atgacgtaat gagtcgcggt aaagataccg
    15181 cagctgtttt cgagacgtca tcgctggtta aagaggattg catatgacca aaggcgctgg
    15241 catcaaggcc aattgcactg gcaatagcca ggccaactaa acctgcaatc atgactaaac
    15301 acaacataca ggcaacaata gttaaagaca tcttgccaat accagcgcta
//
LOCUS       DAEEDS010000086        15345 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEEDS010000086 DAEEDS010000000
VERSION     DAEEDS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15345
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.086"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(174..1199)
                     /gene="tdh"
                     /locus_tag="KLH32_003135"
     CDS             complement(174..1199)
                     /gene="tdh"
                     /locus_tag="KLH32_003135"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBE0139871.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1209..2405)
                     /gene="kbl"
                     /locus_tag="KLH32_003136"
     CDS             complement(1209..2405)
                     /gene="kbl"
                     /locus_tag="KLH32_003136"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBE0139872.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2680..3537)
                     /gene="yibB"
                     /locus_tag="KLH32_003137"
     CDS             complement(2680..3537)
                     /gene="yibB"
                     /locus_tag="KLH32_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBE0139873.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3841..4773
                     /gene="rfaD"
                     /locus_tag="KLH32_003138"
     CDS             3841..4773
                     /gene="rfaD"
                     /locus_tag="KLH32_003138"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBE0139874.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4783..5829
                     /gene="rfaF"
                     /locus_tag="KLH32_003139"
     CDS             4783..5829
                     /gene="rfaF"
                     /locus_tag="KLH32_003139"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBE0139875.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5833..6825
                     /gene="rfaC"
                     /locus_tag="KLH32_003140"
     CDS             5833..6825
                     /gene="rfaC"
                     /locus_tag="KLH32_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBE0139876.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6822..8030
                     /gene="rfaL"
                     /locus_tag="KLH32_003141"
     CDS             6822..8030
                     /gene="rfaL"
                     /locus_tag="KLH32_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBE0139877.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8066..9208)
                     /locus_tag="KLH32_003142"
     CDS             complement(8066..9208)
                     /locus_tag="KLH32_003142"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBE0139878.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9217..10230)
                     /locus_tag="KLH32_003143"
     CDS             complement(9217..10230)
                     /locus_tag="KLH32_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBE0139879.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIVLEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10255..10962)
                     /gene="rfaY"
                     /locus_tag="KLH32_003144"
     CDS             complement(10255..10962)
                     /gene="rfaY"
                     /locus_tag="KLH32_003144"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBE0139880.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10988..11995)
                     /gene="waaO"
                     /locus_tag="KLH32_003145"
                     /gene_synonym="rfaI"
     CDS             complement(10988..11995)
                     /gene="waaO"
                     /locus_tag="KLH32_003145"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBE0139881.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12038..12835)
                     /gene="rfaP"
                     /locus_tag="KLH32_003146"
     CDS             complement(12038..12835)
                     /gene="rfaP"
                     /locus_tag="KLH32_003146"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBE0139882.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12828..13952)
                     /locus_tag="KLH32_003147"
     CDS             complement(12828..13952)
                     /locus_tag="KLH32_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBE0139883.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13949..15007)
                     /gene="rfaQ"
                     /locus_tag="KLH32_003148"
     CDS             complement(13949..15007)
                     /gene="rfaQ"
                     /locus_tag="KLH32_003148"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBE0139884.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4379 a         3447 c         3145 g         4374 t
ORIGIN      
        1 cctgtgagat attattttta caggtgagag agtcacttca gggtagagag ataatgagaa
       61 cagctatata aaagatcagc gcaattacga actctttatt acactgacag gattttacat
      121 aattaacaga ttatcctcag ataaaagggc tggaatacca gcccttgttc gtgttaatcc
      181 cagctcagaa taactttccc ggactggccc gaacgcatag cgtcaaagcc cttctggaaa
      241 tcatcgatag agaaacgatg ggtaatgatc ggcgagagat cgaggccaga ctgaatcagc
      301 gccgccatct tgtaccaggt ttcaaacatc tcacgaccgt aaatgccttt aatgaacaag
      361 cctttaaaga tcactttggt ccagtcgata gacatatcag acggtggaat acccagcatc
      421 gcaatacgac cgccgtggtt catggtatcg agcatggtac gaaacgctgg cggcgcgccg
      481 gacatttcca gaccgacatc aaagccttcg gtcatgccca gttcagccat cacatcatta
      541 aggttttctt tcgcgacgtt aaccgcacgg gtgataccca ttttgcgcgc cagctcaagg
      601 cggtattcgt taacatcggt gatcaccaca ttgcgtgcac caacgtgttt cgccaccgcc
      661 gcagccataa tacctatcgg acctgcacca gaaaccagca catcttcgcc caccagatcg
      721 aacgacagcg cggtatgcac ggcgttaccg aagggatcaa aaatggaagc caggtcgtcg
      781 gagatattgt cggggatttt ggaagcgttg aacgccggaa tcaccagata ttcggcgaag
      841 cagcccggac ggttaacacc gacaccaatg gtgttgcggc acagatgggt acgaccgcca
      901 cgacagttgc gacagtgacc gcaggtgata tggccttcgc cagaaacgcg atcgccgatt
      961 ttgaagcctt tcacttcctg accaatacct accacttcac cgacatattc atggcccacg
     1021 accatcggca ccgggatggt tttttgcgac cactcatccc agttatagat gtgaacgtca
     1081 gtcccgcaga tggctgtttt acggatttta atcagcagat cgttatgccc gagttccggt
     1141 acagggacgt cggtcatcca gatgccctct tccgctttca gtttggataa cgctttcatc
     1201 tcacatcctc aggcgataac gcccagttgt ttaccaatac gcgtgaatgc ttctactgca
     1261 cgcgtaattt gctcaggggt atgcgccgca gacatctggg tacgaatacg cgcctgacct
     1321 ttcggaacga ccggatagaa gaaaccggtg acgtaaatgc cctctttttg cagctctcgg
     1381 gcaaatttct gcgccactac cgcatcacca agcatgaccg gaataatggc gtgatcggct
     1441 cccgccaggg taaagcccgc cgccgacatt tgctcacgga actgacgcgc gttcgcccac
     1501 agacggtcac gcaactcgct acccgcttca accatctcca gcactttgat ggaagcggcg
     1561 acaattgctg gtgccagcga gttggagaac aggtacggac gagaacgctg ccgcagccac
     1621 tcaaccactt ctttgcgcgc cgcggtataa ccaccagaag ccccgcccag cgctttaccg
     1681 agcgtgccgg tgatgatgtc gacacgcccc atcacatcgc agtattcatg ggaaccacga
     1741 ccattttcac cgacaaaacc gaccgcgtgg gagtcgtcta ccatcaccag ggcatcgtat
     1801 ttatctgcca gatcgcaaac gcccttcaga ttggcaatca cgccgtccat cgagaacaca
     1861 ccatcggtgg caatcagcac atgacgcgca ccggcttcac gcgcttcttt cagacgagct
     1921 tcaagctcct gcatatcgtt gttggcatag cgatagcgtt tagctttaca cagacgcaca
     1981 ccatcaataa tagacgcgtg gttcagtgcg tcggagataa tggcgtcttc cgcgcccagc
     2041 agcgtttcaa acaggccacc gttagcatca aagcaggaag agtagagaat cgcatcttcc
     2101 atccccagga atgccgccag tttttgttca agctctttat ggctgtcctg agtgccgcaa
     2161 ataaaacgca ccgaagccat gccgaaaccg tgagaatcca ttcccgcctt tgctgccgca
     2221 atcagatccg gatgattcgc cagcccgaga tagttgttgg cacaaaagtt aatgacgtgg
     2281 cttccatcag ccacggtgat atctgcttgc tgcgcagacg taataatgcg ctcttcttta
     2341 aacaaccctt ccgcccgtgc ggtttccaga tcgttggtta actgctgata aaattctcca
     2401 cgcattgcga ttctccagac agggcaaatt ccagcacata ttacccaaac ttataggtcg
     2461 cgacgagatg atgcgttaac acttctgcaa aattcaggat aaataacgtg tatcttgatg
     2521 tctctcaaca cctacagata gtagaaaaac tgtacagttt tggctgagca taatgatgag
     2581 tgattttact gcattgatat aaaaagaaaa aatgatgcca aagcatcatt tttcctatct
     2641 cagttggcat caaaacgcta cccactaatg gagggaaacc tattttcttg aaaggaaaat
     2701 tctcagcgcc tgcatttttg cccctaaagt gttactcctg aagcagcgaa acaaatcgaa
     2761 ccattttcct ctccccagat agtttaggtt gaagagatcg gaacgtttgt aatagcacat
     2821 gacaaaaatg ccctggtcat cgtccacaat gttattgttt agtgttattt tttgactttc
     2881 cagaactaat ttatagaatt ctttccattt atgctgtgaa ccaacaattg cgcccccaat
     2941 aatatataca tgattgccaa tcatgaaatc aaaagcctgt tgttgagatg taacagtaag
     3001 cccttttttt atagtgaaaa gatgcatctt attttcatca aatgggaaat cccaaatttt
     3061 caatcctcga gttacatttg gcttacggca atagccaaaa tctatccacg caacaagagg
     3121 cgttttcact aaacccatgt tgatagcttt atttacaaaa taggccttaa ggttacatac
     3181 caaaacatac tctggtgacc agtactctgg gttttttaat tgtcgaggct caagtctatt
     3241 tgtaaatgac tcatcttttt gaattttttc gatacgactt ctgatatatc taaatttctt
     3301 ttttatatcg ataacgataa ccgttgttgg ttttccgttg cgaatcgctt caatccttgg
     3361 tttcaaatcg ggagaagtga aaataatcat ctcattttca agtgcggcca gacgctcaaa
     3421 gtaactaaaa taaacatcaa ctgaacgagc tagtttttca cggaaccctt tattagcagt
     3481 ccaatcccct ctaccaatgt caaaatatgc agtgataatt gtggtggatg atttcattac
     3541 taataagacc attaatataa aaaaagaatt atatcataaa tattaacaga atgtatactg
     3601 tatattatcc attcaaatga ttccgttatt tatcatacat cggaatattg atactaaagc
     3661 actattcaca tgcaaaacca acatccgcca tgaaggacta gctaaaaccc aaactagttt
     3721 gttgcaatta gcatccttgc acctctatgt aaagggctga agggattcgg atgtgatggt
     3781 atgattacag acattcgtgt ctgagattgt ctctgactcc ataattcgaa ggttacagtt
     3841 atgatcatcg ttaccggcgg cgcgggcttt atcggcagca acatcgttaa ggccctgaat
     3901 gataaaggca tcaccgatat tctggtggtg gacaacctga aagacggcac caagtttgtg
     3961 aacctggtgg atctggatat cgcggactat atggataagg aagacttcct gatccagatt
     4021 atggctggcg aagagttcgg tgatgtcgaa gcgattttcc acgaaggcgc atgctcttcc
     4081 accaccgagt gggatggcaa gtatatgatg gataacaact atcaatactc caaagagctg
     4141 ctgcactact gtctggagcg cgaaatcccg ttcctgtatg cctcttccgc agccacctac
     4201 ggcggacgca cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt gaatgtctac
     4261 ggttactcaa aattcctgtt tgatgaatat gttcgtcaaa tcctgccaga agcgaactcg
     4321 cagattgttg gcttccgcta tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc
     4381 atggcgagcg tcgctttcca tctcaacacc cagcttaata acggtgaatc accgaagctg
     4441 ttcgaaggta gcgagaactt caaacgtgac ttcgtctatg tgggcgacgt ggcagatgta
     4501 aacctgtggt tcctggaaaa tggcgtttcc ggtatcttca acctcggcac cggtcgtgca
     4561 gaatccttcc aggcagtagc agatgctacg ctggcttatc acaagaaagg ccagatcgaa
     4621 tacattccgt tcccggataa gctgaaaggc cgctaccagg cgttcactca ggcagatctg
     4681 acaaatctgc gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg
     4741 gaatacatgg cctggctgaa tcgcgacgca taagagctct gcatgaaaat actggtgatc
     4801 ggcccgtctt gggttggcga catgatgatg tcgcaaagtc tctatcgcac gctccaggcg
     4861 cgctatcccc aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc attattatcg
     4921 cggatgccgg aagttaacga agctatccct atgcctctcg gtcacggagc gctggaaatc
     4981 ggcgaacgcc gcaaactggg tcatagcctg cgtgaaaagc gctacgaccg cgcctacgtc
     5041 ttaccaaact ccttcaaatc tgcattagtg cctttcttcg cgggtattcc tcatcgcacc
     5101 ggctggcgcg gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct cgataaagaa
     5161 gcctggccgc taatggtgga acgctatgtc gcgctggcct atgacaaagg cattatgcgt
     5221 accgcacaag atctgccgca gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa
     5281 aaatcatata cctgtaatca attttcgctt tcatcagaac gtccgatgat tggcttttgc
     5341 ccgggtgcgg agtttggtcc ggcaaaacgc tggccacact accactatgc ggaactggca
     5401 aagcagctga ttgatgaagg ttatcaggtg gttctgtttg gctctgcgaa agatcatgaa
     5461 gcgggcaatg agattcttgc cgctttgaat accgagcagc aggcatggtg tcggaacctg
     5521 gcgggggaaa cacagcttga tcaagcggtt atcttgattg cagcctgtaa agccattgtc
     5581 actaacgatt ctggcctgat gcacgttgcg gcggcgctca atcgtccgct ggttgccctg
     5641 tatggtccaa gtagcccgga cttcacaccg ccgctatccc ataaagcgcg cgtgatccgt
     5701 ctgattaccg gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc
     5761 ttgatcgaca ttactcccca gcgcgtactg gaagaactca acgcgctatt gttacaagag
     5821 gaagcctgac ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga tgttctccat
     5881 acgttgcccg cactcactga tgcccagcag gcaatcccag ggattaagtt tgactgggtg
     5941 gtggaagaag ggttcgcaca gattccttcc tggcacgctg ccgttgagcg agttattcct
     6001 gtggcaatac gtcgctggcg taaagcctgg ttctcggccc ccataaaagc tgaacgcaaa
     6061 gcgtttcgtg aagcgctaca agcagagaac tatgacgcag ttatcgacgc tcaggggctg
     6121 gtaaaaagcg cggcactggt gacacgtctg gcgcatggcg taaagcatgg attggactgg
     6181 caaaccgctc gcgaaccttt agccagcctg ttttacaatt gtaagcatca tattgcaaaa
     6241 cagcagcacg ccgtagaacg cacccgcgaa ctgtttgcta aaagtttggg ctatagcaaa
     6301 ccgcaaaccc agggcgatta tgctatcgca cagcattttc tgacgaacct gcctacagat
     6361 gctggcgaat atgccgtatt tcttcatgcg acgacccgtg atgataaaca ctggccggaa
     6421 gaacactggc gagaattgat tggtttactg gctgattcag gaatacggat taaacttccg
     6481 tggggcgcgc cgcatgagga agaacgggcg aaacgactgg cggaaggatt tgcttatgtt
     6541 gaagtattgc cgaagatgag tctggaaggc gttgcccgcg tgctggccgg ggctaaattt
     6601 gtagtgtcgg tggatacggg gttaagccat ttaacggcgg cactggatag acccaatatc
     6661 acggtttatg gaccaaccga tccgggatta attggtgggt atgggaagaa tcaagtagag
     6721 tgtcgttcta caagtatgtc tcttgcagat ttgccagctc aaaccgtttt tcaaaattta
     6781 aatcttgaaa taataaccaa taagttgaca tcggagataa gatgacctca acattatttt
     6841 tctctctcga gaaaaaaaac tggatagcgt actggaacag agctctcgta ttcttattca
     6901 ttaccaccta ttttttgggt gggattacaa ggtataaaca tcttattgtt attcttatga
     6961 caataacgac aatcgtctat ctctgcaaac ggccaaaaca ctatctctca ctatttaaaa
     7021 catttctttt tggtagtgtt gccatattaa ctattgctgc attgctgtca cttcttcaat
     7081 cccctgatgc aggtgctagc atgaaggaag ttttcaaagc tattattgag aatactttac
     7141 tatgcacaat agcaataccg gtcatattga gagacgagaa aagagaagat gtcgaaaaaa
     7201 tcgttttctt ctcatttatt agtgcgttgg gcttacgctg tttttctgaa ttgattacct
     7261 attataagga ctatcaacaa gggataatgc cattcgcaga ttatagacac cgtagcattt
     7321 ctgactcgat ggtcttttta ttccctgcat tgttaaatct ctggcttatc aaatcagcaa
     7381 aataccgcat ttcttttgtg gttctaagcg ttatttttat ttttctgata ttaggaactt
     7441 tatccagagg ggcctggctt tccgtgttag tcattggatt aatatggatt ctgatgttta
     7501 aacaatggaa gttactatta gtaggagtaa tggtttccat cattgcattg tcggttattt
     7561 tcacacataa ggagatgacc gcaaagctaa cgtataaact tcaacaaact aatagttctt
     7621 atcgctatgc aaatggtact caaggcagcg cactcgatct aatattagaa aatcctgtta
     7681 ttggttatgg ttacggtaac gttgcatata aagatgtcta taataaacgt gtcattgatt
     7741 atccagaatg gacctttaga caatcaatag ggccacataa ttttgcgcta ttcatctggt
     7801 ttggcactgg tttattaggg ctggtaagtc ttatgatgct atactgtgca atattgaaag
     7861 agtgtataaa aaatggcgtc aagaataaat atcgctcacc atataatgca tattatataa
     7921 tcttactatc ttttataggt tattttgtta tccgtggaaa cgtagaacaa attgaaccaa
     7981 atttattagg cgtttacgcc ggcttattat tagcgatgaa aaacaagtaa aaaataaaaa
     8041 aggctgcata atgcggcctt tttatttatt caaaccaatt atgaataacc tcttcgaatc
     8101 tttgagttac gccatcccaa ctgtagtgat caaacacaaa gtcctgacct tgctttgcaa
     8161 ctgctgttaa ttcaggattc gctaacgttt ttaaaatatc gctgcttatg gagtctgcag
     8221 tcataggttc ttttaaatgg aatcccgtag tattttcttt gacaaattcc gtcatccccc
     8281 cacgcgtact cactaaaaca ggcttaccag cacccatagc ttcaattgcc accatgcaaa
     8341 atggttcctg gaactgggaa ggaatgacga ctaaatctgc cagtgggtaa taacaataca
     8401 tcttttcagg cggtatgctt cctaacataa tgcaacgatc cttcaaacgt tttgcaattt
     8461 ccctgacatt gcgttgatat gcacctttgt cacttttact catttctgta taatcaccaa
     8521 tcacaactaa ttttaaattt ttgtgagcag cggccaattt ttcgaatgat tgcatcaaca
     8581 gcaatatgcc tttgtcaggg acgatacgcc cagcataaaa aataattttc tcttcaggag
     8641 agatattaag ttcgcttcta gtgatcggct gaaaattaga ttgataggtt tctaaatcaa
     8701 ttccatttgg tacaatctca atatctgcat ttgctaaata agattggtaa tactttttca
     8761 agtataaact agggactatc attttaacat tttgctcaag caattttggt tcaaatgcat
     8821 tatgcatatg tataaccact cttgcctgag gagctagctt acgaatttga gtgtatagct
     8881 tcatgctatt atgaactaca ataacacttt ctttcgttat aggaaaatca tgtgcaatat
     8941 tcaatattct ctgtgaatat ggaagaggat caagtcttgt ccatttttga aataaccgct
     9001 tatatatgcg actgaatcca accctatgaa tactacaatt atcattaaca aaggtgtaat
     9061 ttgagtatcc tggatttttg atacaaacaa tacgatttgg aaaattggta cgctgagcaa
     9121 cctgatacat ccacgtttca acagcagccg ctccgcgagg aggaattgaa aaaataggag
     9181 taaccgtaaa tattatttta tcaaccatct cgtatattaa cctttcataa cattatattt
     9241 aattgctgta aaaacaccat cgagaaattt tttctggtaa agcaagtgtt tatatttttc
     9301 tttgtgctca tgttttttaa cagctttttt ataaggtata tttctccatg gtgatatatt
     9361 ataaatatta cgaaaataat ctgcagaggc gtagttcgcc caatcatgcc atggcttagt
     9421 tataccagta taatgtatga agacagtgtc atcattaata aaccgggtgt aatattcact
     9481 atttttttct tcaaattctg actttatagt ataaatacaa ttatattttc tcggtaatat
     9541 tttagcatga tgtagaaaca ttaagtttaa aatatcttga tccgggtact ttaatttttt
     9601 tataattgac tcatctgata atagatcaaa gaatttttcc gttagtcttt gttttaacca
     9661 ttcccgtaaa ttaatataca ttacaccaga gttaaaatag ctaccgttaa aatcctcatt
     9721 acacaaacgc tcagcgcttt tactttgcat agcatctaca tcaattacca ctgccccata
     9781 ttcatcttta aactctaata ctattaactc gttcaatgaa cccttacaga cgatatcagc
     9841 atcaagatat aataatgaat ccaatcgcgc agaaaaataa tcaaatgaaa gcaaacgata
     9901 ataaatagat actggccaga tatttgattg aggtagcgcc ttcaatggct cagaatcaat
     9961 atgatataat ttaattactg tttgatactt cgttgctaat tgtgataata attcaatata
    10021 ctctttgtct aagtaatcag cgattatgtg aaaggtaaat ttgttttgtt tattcttttc
    10081 aagaattgaa gcaattgaaa tcgcagcccc aacctggtag ttctcatcaa taccccatga
    10141 gacattaaac gtttcccgtt catcaagttt aacggggcgc ttgtccagtt caattatatc
    10201 tttaaattga gtaagatgtt taaaatccaa tttaattacc cttcatcata atcattattt
    10261 gctcttcaat cctagtttga cttttacatc cctgattact tttttaattt tctttttgaa
    10321 tataagatat gtatagccaa agtctttcaa ttcatttttt atattatagt gccgttcaag
    10381 atcaatgcga tcttttgctt ttaaaacagc ggttgttttt ttacctgaaa ggtcaattag
    10441 tcttaaacct ttttcggata ctataaaatt tcctttatga ggatcgccag acaccattcc
    10501 atgctgatgc aactctttta tcgattctga taattgatcc ttcagatctt cagaaatttc
    10561 caaatactca tttaacccga caccttcaat atactcaatg agcataatat aataatgagc
    10621 gaaatttaat gttttacgtt cagctagcaa aaaatagtca ttaattgatt ggatgccttc
    10681 accacgtacc cgatcggttt gataaattaa attttcataa taatcttttt ttatgcaaga
    10741 ttttaagaaa cgttcggtca ttttatgctt tggcgcataa actttaagta caagaggtcc
    10801 acgagcagtg tcaataagaa tgactttagt atcatcaata gaacgaaaaa cttttaatgt
    10861 tttaaccctg caagttaaaa agtcatttag aacttgctca taaaagggat cattatcttt
    10921 tatgaaaact ttcaacccat ttatcgtttt attatatatc ataatgaatt attttaacct
    10981 taaatcttta gaagcatttt tctttataat actttaaata attaaaaatg cctgccatat
    11041 aatgcttttg tttaaattta tgttttgcac aataccgata ctgattgcta tttacaggtt
    11101 taagtaaatc ttctttactc cacggagaag ctgctttggc gatcaaaaaa ctacgtgaaa
    11161 ccggataatc agcccactca tgccagggtt ttgtaggtcc cacatagtgt ataaacacgg
    11221 tgtcatcatt gactggatta tcaactttgt cttttaattc atagttaata ctatatcggg
    11281 tattatattt ctccgaaata aacttcactt taccattcaa taatacattc agtacatctt
    11341 gatcaaggtg ggtgatttta cttacccaat ctgggtcacg caacatttca atagcttttg
    11401 acgaaatgtt atttagattc cactcatcaa tatttatcag taaaaaacca gcattgaaat
    11461 atccagaagc taactgtggt gtagttaata ccgaggctcg attttgccac cattcaatat
    11521 ctctttcagc tacaactgcg gcaatttcat tagtagaaaa ttgataatct aagagttctt
    11581 taatactacc cttgcaagca atatctgcat caagatatag tattttttca tgtttatgat
    11641 agaaataatc tgcaattata aatcgaaaat atgttgcgta agtccagttt ttcgtgcttg
    11701 gtaatgactt cagcttatca caattgataa cataaatatt aattcttgaa ttatattgtt
    11761 ttgctaaatc agaaaaatat aatttgtctt tatcactgat ataatctgtg aaaacatgaa
    11821 attcacaaga gatttctcta ttgtttaata gaatagatgc gatggctaca ccacatccaa
    11881 aaagaaaatt tctgtcaata ccaaatgcta tatcaagagt atttttagat cttatatttt
    11941 gataatgaaa attatactca aaaaggatga tgtcactatc attgagttga gacatattta
    12001 ttctgtatcc aaaattttgg caaaaataag ttacgggtta caacgatttt cgaatcgttc
    12061 tttccctgat ttttgtggct tttgcttctg cttgcgacag cagtccttgt tcctgcttga
    12121 gaatgtcttt aagcggggcg gcaaaataca ccttcataaa gcgccagata tcccgctgag
    12181 tcaggccaat attcattgaa gaaaaataaa gccctataag atctttatcg cgccaacgac
    12241 ctggaacgcg cgtgcgaagc tgcgcccggt gtaggtcaat taccgaaatt tttaactcct
    12301 cttccttacc ggaaaaaggc aagtgcagca ggaaatgaca gatataacag tcacgatggt
    12361 taatgcccgc agcatgcata tcgcgcacca tcgtcgcgac acgcttaata agcatacgct
    12421 ttacgcgaac atctggtggg ttggtcgccc agtcagcaca gtaatcttcc agactgatgg
    12481 ttggtgtcag atcttcggta ataataaacg aggttctggt cagcggattc atgccttttt
    12541 cgccgaatgc caccccatac atagtatcaa cgccgacatc ccgcagtcga tgaatcgcat
    12601 tccattcgcg gtctgcgcct aatactggca tccgcaatga gagtaaattt ttgattatct
    12661 ctttcagggt cgtgccgcga tgccatttga gaaaatagct ttttcccgcc atttcaaagc
    12721 gcagagtacg gcgagtttcc agttcacgaa atacctcacc ctgcaaggtt ttaacttcct
    12781 caaaaggatc tttgccgcgc cataacgtgg caaacggctc tttaagttca accatccaga
    12841 ccacccgtta tgatatccgc cgctttctct ggcagactgt ataaatcttg tgtatcagca
    12901 taatgtcgcg cattttccgc ccaagcctgg cgcaatgaag attgcgttaa cgctttgcgt
    12961 aaaatctcat tcaatgtttc ctggcggaat ggctcagcaa tagcctcgcc gcaattagcg
    13021 tcgacaatat aatgcgcata gccacaaacg gcagttgtta gtaccggtaa tcctgcagta
    13081 atcgcttcca gcagcacaat tcccgccgct tcctggtagg caggatgcag taataaatcc
    13141 gccgccgcca ttaattccga gacatcgttg cgccccgaga agaagtgaac attactgcgc
    13201 acgccgcgtt tttctgccag tgcctcaaat tttcgcggtt tatcctgccc aacaacatat
    13261 agcaatgtgt tgtggcgcag cgaatccggt aacgaagcaa gtgcttcaat ggaacgatcg
    13321 gcacctttac gcgtgaagtc tgaaccgacc tgcaacaata aatattgttg ttcggttatt
    13381 ccattcttct tacggaagat ttcacggcta ttggctggct gctggctata tttacgatca
    13441 ggataaatcc ccggtggcag aatatgaaaa cgctccgctt cagtctgata atgtttctgg
    13501 aaatcggcga tttgcttatc tgtcagcatc agcagctgtg tcggcttgcc ctgttcgaag
    13561 gttgcccgct caaaggcggc ataatggcga taacgtgacg tcaggcgata gaaaaagcct
    13621 ttttcctgcg ctactttctc ggcataacaa acatcagcgg cataataaac gtccagcccc
    13681 ggcattttgt tgaatccaac gactttatcg acgggatgtt cgcgtaaatg tttttgcacc
    13741 caggcaaaat actccgcatt gcgcccgtga ttggtatgcg atttaaccgg cactttgatc
    13801 acttcaaata catcagggca ttcgccttcc cacgactggg tataaacccg aacatgatga
    13861 cctcgggcgg cgactgtctg agcaatacgc ataaaatcgc gctgcaaacc gccaaaggga
    13921 aaatatttat ataaacaaaa agcaacgatc ataacgacgt acctgtcgtg gaggagggca
    13981 gtaatttatc gacagcagca atgacatctg ccgccggaat aaccgaaaga tacatctcat
    14041 ttcggtcacg ctgatcgcgc gttggcattt cccggtaatc tcccgcccag aattgaatca
    14101 tgttatttga ccagggacgc cagaaaatat gatctgttgc gccaaacagc gatatcagcg
    14161 gcgtattaac tgcagcggca atatgcgccg gtgcggaatc aacgccaata aacagctgcg
    14221 catgatcgat taacgcacca agttccggga aggtcacctt tccagccagc gccgttactg
    14281 gtggcgtctg gcatccctgc gcaatttcat tgacgcaggc cagatcgtct ttatctgggc
    14341 cggacgtcag aacgacttca taaccacgag catgtaaggc atcaatcaca gcggaaaact
    14401 tggcgttgtc ccagcatttg aagatttgcc gcgccgtagg ttggataacc acataatttt
    14461 gtccaacacc agcgtgatca agttcgcgac gcatacgttt ccagcttgca ggcgggtaac
    14521 tcatggttgt ctgcttcacc aacgaatcaa gtcccaatgg tgtcagcacg gataagttac
    14581 tttccaccac atttccaccc tgcaacggca ccaaatgggt gaaactttta cgccaaaaag
    14641 cagactgccg atgatgataa tcctgggaaa ttttcacacg agcatttaat aagcgaacca
    14701 gtatagcaac catccattga tcggtaagat tgacgataag gtcatactta ttggcacgta
    14761 ataccttgat gagatgaaaa aagttggcaa ttttttctga ggcttttgct tttttatttt
    14821 ttatgccgta gagcgcgtta atctctggat tttcagacag gatcgggatg gtgtcctgat
    14881 aaagcagcac atcgattttt gcgtcagggt aatttttttt cagagaacta atgacgggag
    14941 tagttaataa catatcccca tgaaaacgca tcttaatgag caaaattctt cgaaatggct
    15001 tatccacaag taactctttt tgttgagatt atccgattaa ggtgaagcag aaaaagcacg
    15061 ctaccgcccc aggctcaaca gctacctgaa tactgatacc gcactgatat tgacgcgcta
    15121 gtgtatcact tctttcgcgg caatccgacc cgaatgattt ttaagcacat tgttgatttt
    15181 caataaagaa atcaatcaaa taaaatcatt agcaaccata ttctttcact gttgctctgc
    15241 gattccttcc tgcacgtaca gcagaaatca atgaatcgca taaaaataca aaaaaagtat
    15301 agatgttcag caagaattgg cgggtaaatt gctatttaag tcaaa
//
LOCUS       DAEEDS010000087        15099 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEEDS010000087 DAEEDS010000000
VERSION     DAEEDS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.087"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..179
                     /gene="yjbD"
                     /locus_tag="KLH32_003149"
     CDS             <1..179
                     /gene="yjbD"
                     /locus_tag="KLH32_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBE0139885.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KLH32_003150"
     CDS             complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KLH32_003150"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBE0139886.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1532..2479
                     /locus_tag="KLH32_003151"
     CDS             1532..2479
                     /locus_tag="KLH32_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBE0139887.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2549..3355
                     /locus_tag="KLH32_003152"
     CDS             2549..3355
                     /locus_tag="KLH32_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0139888.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3365..3772
                     /locus_tag="KLH32_003153"
                     /pseudo
     CDS             3365..3772
                     /locus_tag="KLH32_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3772..4266
                     /locus_tag="KLH32_003154"
     CDS             3772..4266
                     /locus_tag="KLH32_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBE0139889.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4332..5129
                     /locus_tag="KLH32_003155"
     CDS             4332..5129
                     /locus_tag="KLH32_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBE0139890.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5140..5928
                     /locus_tag="KLH32_003156"
     CDS             5140..5928
                     /locus_tag="KLH32_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE0139891.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5980..7209
                     /gene="sorE"
                     /locus_tag="KLH32_003157"
     CDS             5980..7209
                     /gene="sorE"
                     /locus_tag="KLH32_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBE0139892.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7591..8280
                     /gene="pepE"
                     /locus_tag="KLH32_003158"
     CDS             7591..8280
                     /gene="pepE"
                     /locus_tag="KLH32_003158"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBE0139893.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8371..10002)
                     /locus_tag="KLH32_003159"
     CDS             complement(8371..10002)
                     /locus_tag="KLH32_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBE0139894.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KLH32_003160"
     CDS             complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KLH32_003160"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBE0139895.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14105..14929
                     /gene="iclR"
                     /locus_tag="KLH32_003161"
     CDS             14105..14929
                     /gene="iclR"
                     /locus_tag="KLH32_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBE0139896.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3982 c         3937 g         3589 t
ORIGIN      
        1 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
       61 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
      121 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
      181 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
      241 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
      301 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      361 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      421 cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg
      481 agcttaaaga ggtcgattaa ctcatcgtcg gttaaatagc gccactcccc cagcggaatg
      541 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      601 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ctagggtaat gcggaagaca
      661 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      721 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      781 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      841 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      901 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
      961 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1021 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1081 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1141 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1201 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     1261 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg
     1321 cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta
     1381 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     1441 aatcgctttg aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat
     1501 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     1561 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     1621 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     1681 ggtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     1741 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     1801 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     1861 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     1921 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     1981 cgaaagccgc tatcatgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga
     2041 atcgcatctc gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat
     2101 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggata tcgccctggt
     2161 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     2221 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     2281 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acgctctcta tcgaaatgaa
     2341 taaattaaag caggcgcgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     2401 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     2461 tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt
     2521 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     2581 cattattgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     2641 acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggtcaat atgaaggtca
     2701 taaaggctat cagttttggc cgaccgatat ttctagcaca aaagaggtaa atcatacggt
     2761 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     2821 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     2881 tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt
     2941 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     3001 gctggaaggc tcagaaggac aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     3061 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     3121 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     3181 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     3241 gcgcgccgga agattagcag aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc
     3301 cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg
     3361 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     3421 ggttcagatg gtttacggca acgccaatgt tgaagccgtt gcatttgtgc ccggtgaaaa
     3481 cgccggagac attgcggtaa agctggagaa gttagtaagc attcacaatc aagatgagtg
     3541 gctgattgcc gtggatttac agtgcggcag cccgtagaat gcggcagcaa tgcttgcgat
     3601 gcgcaatcct cgtttgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt
     3661 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     3721 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     3781 acgctcgccc gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa
     3841 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagtgcgc
     3901 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     3961 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     4021 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat
     4081 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     4141 gatgatgata ttaacgcatt tcatgagtta aataatcttg gtgtcatact ggatttacgc
     4201 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     4261 aattaaataa tagcgcctgt gtacatatgc tttaacaggc atggatgacg acacttaaaa
     4321 ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta
     4381 ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta
     4441 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggc ggtacgctgg
     4501 aattgatagc tctctgctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     4561 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     4621 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca
     4681 ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga ggcgcggttt cgcactctcg
     4741 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     4801 ttgtctcact gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     4861 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     4921 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     4981 gtggctacct cgatctcagc ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     5041 tctacatcca gctaaatcca cagtggcgta aagctgaacc tcaactccag gccaccgcct
     5101 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     5161 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     5221 gaacgtattc acgggctggg cttttgctac gacatgatcc ccgccatcaa gcgactttac
     5281 ccattaaaag aggatcaggt tgcggcgctc aggctacacc tagtgttctt caataccacg
     5341 ccagccgtat gtggcccggt catcggcgcg gaaattgatg acggtaccat caacggcatc
     5401 aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg gggaacgctg
     5461 cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat tctcggcccg
     5521 ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag
     5581 ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa
     5641 ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg
     5701 acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc
     5761 accatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc gctcggtctg
     5821 acgctactaa tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc
     5881 ctgtttggct tagggattat cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg
     5941 gcacgactgc cgaggccatc gctcaacatg aggtggttta tgaaaacaac agctctgcgt
     6001 ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat
     6061 gaaatcctcg caacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat
     6121 ctgggtgaaa accataaaaa agtacccgac gatgtgacga ccaacccaat catcatcggc
     6181 cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
     6241 ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga ttgccccggc
     6301 tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg
     6361 gaacaatatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc actggttgaa
     6421 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
     6481 aaccacacga tggggattcg cccgcaaggg cgcacgctga tcctcggcgg caccggacca
     6541 atgggactgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
     6601 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
     6661 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
     6721 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
     6781 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
     6841 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
     6901 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa
     6961 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
     7021 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
     7081 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
     7141 caggggatct ggtccggtga ggcggagcaa tacctgctcg cccatgcaga ggcaatttcc
     7201 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgcccca gcaacttcgc
     7261 gaccggatgt gcggctgatt gctgaagcag cacaatttgc acgagaagga taaaaaatat
     7321 cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg
     7381 aatgaagcct tattcggtct acataatttt gaaaatccaa tatattgcag ttatctggta
     7441 ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt
     7501 gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     7561 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     7621 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     7681 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     7741 gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     7801 gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact
     7861 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt
     7921 aaacgtggcg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     7981 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     8041 ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc
     8101 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtctg
     8161 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     8221 acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca ccgtttttaa
     8281 gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     8341 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     8401 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     8461 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgtacgtttt gctgatgcag
     8521 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     8581 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     8641 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     8701 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     8761 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcctc
     8821 cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt agagaacgtt
     8881 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     8941 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     9001 gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca tccagatgtt taggccgtag
     9061 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     9121 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     9181 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     9241 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     9301 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagctcc
     9361 aatcaccaga cagagcgcca cggggaagga gataatgcct gcagcggtca gagtcgcggt
     9421 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     9481 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     9541 gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat
     9601 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     9661 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     9721 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     9781 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta ccagtgcggt
     9841 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     9901 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     9961 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag gttctcctta
    10021 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
    10081 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
    10141 ataagtgttt catattaata aagagacagg gcactcatta agcgccctgt ttgttgtaag
    10201 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
    10261 ctcaacatcg gagacgctca tacctttacg gcgggcatag tcttcaacct gatcgcgctg
    10321 aatttgcgcc accgcgtagt acttgctgtc cggatgactg aaataccaac ccgaaaccga
    10381 tgcaccaggc cacatcgcga aagattctgt gagtttcatg ccagtatgtt tttccacttc
    10441 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc
    10501 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
    10561 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
    10621 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
    10681 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
    10741 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
    10801 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
    10861 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
    10921 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
    10981 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact tcccggccag
    11041 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc
    11101 ttccacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
    11161 tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg
    11221 cccgtgctga atacgcacgg tttcgtactc cttgcaggta cgagcgacaa aatcatcacg
    11281 ctgagtatcg gaaagcagcg ccgctaccac accaacggtg cgcgaggcat tctgcacata
    11341 caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt
    11401 cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc tccatctctt tcgccacgtt
    11461 aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcaaat cagcattcac
    11521 ttctttggcg atacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
    11581 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacatcgcc
    11641 tttcacggtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat
    11701 aaacggttcg agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg
    11761 caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
    11821 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc
    11881 gataaactcg gttatgcctt tgaccagcga gtattccaga cgtttattca cgtcccacga
    11941 gcgccactcc gcttgctggg cgttagcagt gtcgtccgct ttactgccgc gatatttctc
    12001 ggcaagctcc agtaaacgct cagtgccatc gtcacgacga ttaagaatca catcttccac
    12061 cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat
    12121 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
    12181 caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg aaatcagcgc
    12241 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
    12301 gtgctcttca ataccggtgg cgaccgcgaa gatgttcggg tcgaagatga tatcttctgg
    12361 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
    12421 tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc accaccactg ccgcaccgta
    12481 gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
    12541 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
    12601 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
    12661 acgcaccatc gccgcttcgg catcgagcat cccttcatcc atgttgatat cgataatctg
    12721 cgcgccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt tctcttcttt
    12781 gatcaggcgc ttgaacttag cagagccggt gacgttggtg cgttcaccca cgttcacaaa
    12841 caggctatcg tcgccaatgt tcagcggctc caggccagac aaacggcagg ctacgggaat
    12901 ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
    12961 cggcgtggtc ccgcagcaac caccgacgat attgagaaag cccgcttgcg cccattcacg
    13021 tatctgtttt gccatcgtgt cggcgtcgag atcgtattca ccaaaggcgt taggcagccc
    13081 ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt gatagttcct gcacgtactg
    13141 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg
    13201 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
    13261 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
    13321 cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
    13381 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccgcca gctggtcaaa
    13441 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
    13501 tgggccaaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
    13561 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
    13621 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc
    13681 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac tgagtaccag
    13741 caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc
    13801 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca acaccagaat
    13861 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
    13921 gctcaaccca gatttaataa aaattcaaca aaccatactg gaataaacgc atctgtggta
    13981 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
    14041 actattgcat tagctaacaa taaaaatgaa aataatttcc acgatacaga aaaaggaggc
    14101 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
    14161 agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
    14221 atccaatggc agcgtggcac tcacagagct gtcgcaacaa gccgggttac ccaattccac
    14281 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
    14341 gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
    14401 taatttgtta gcaattgttc accctatcct gcgcaatcta atggaagagt ctggcgaaac
    14461 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
    14521 gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
    14581 aggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
    14641 ccgcaaaggg ttacatgcct atacccacgc aactctggtg tctcctgtgc atttaaaaga
    14701 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg
    14761 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
    14821 tatttccgga ccgatttcac gtattaccga tgatcgcgtg accgagtttg gcgcgatggt
    14881 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
    14941 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
    15001 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta ttaactcagc
    15061 attattttta aacatcaaac cacttaatta ttaacaaaa
//
LOCUS       DAEEDS010000088        15052 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEEDS010000088 DAEEDS010000000
VERSION     DAEEDS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.088"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..177)
                     /locus_tag="KLH32_003162"
     CDS             complement(<1..177)
                     /locus_tag="KLH32_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139897.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHGL"
     gene            complement(593..1534)
                     /gene="fabY"
                     /locus_tag="KLH32_003163"
                     /gene_synonym="yiiD"
     CDS             complement(593..1534)
                     /gene="fabY"
                     /locus_tag="KLH32_003163"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBE0139898.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="KLH32_003164"
     CDS             complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="KLH32_003164"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBE0139899.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2013..2885)
                     /locus_tag="KLH32_003165"
     CDS             complement(2013..2885)
                     /locus_tag="KLH32_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBE0139900.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="KLH32_003166"
     CDS             complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="KLH32_003166"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBE0139901.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3577..4362)
                     /locus_tag="KLH32_003167"
     CDS             complement(3577..4362)
                     /locus_tag="KLH32_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0139902.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="KLH32_003168"
     CDS             complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="KLH32_003168"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBE0139903.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5460..6356
                     /gene="yihU"
                     /locus_tag="KLH32_003169"
     CDS             5460..6356
                     /gene="yihU"
                     /locus_tag="KLH32_003169"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBE0139904.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6380..7258
                     /gene="yihT"
                     /locus_tag="KLH32_003170"
     CDS             6380..7258
                     /gene="yihT"
                     /locus_tag="KLH32_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBE0139905.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7275..8516
                     /gene="yihS"
                     /locus_tag="KLH32_003171"
     CDS             7275..8516
                     /gene="yihS"
                     /locus_tag="KLH32_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBE0139906.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8654..9556
                     /locus_tag="KLH32_003172"
                     /pseudo
     CDS             8654..9556
                     /locus_tag="KLH32_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9755..11791
                     /gene="yihQ"
                     /locus_tag="KLH32_003173"
     CDS             9755..11791
                     /gene="yihQ"
                     /locus_tag="KLH32_003173"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBE0139907.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11837..13211
                     /locus_tag="KLH32_003174"
                     /pseudo
     CDS             11837..13211
                     /locus_tag="KLH32_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13254..14657
                     /locus_tag="KLH32_003175"
     CDS             13254..14657
                     /locus_tag="KLH32_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0139908.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14725..>15052
                     /gene="ompL"
                     /locus_tag="KLH32_003176"
     CDS             14725..>15052
                     /gene="ompL"
                     /locus_tag="KLH32_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBE0139909.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3699 a         3875 c         3745 g         3733 t
ORIGIN      
        1 caaaccatgc ttcagttcgt taacacaatc gcacagttgc tcctcacctt ccattttaag
       61 gcaaagtgaa aattgattta atagcgtttg cttcggtgat ttattactca ttagttgttt
      121 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      181 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      241 agtaaatgaa tgatactgac aatgtatgcc agcccattat ttttgcataa tatttgtgag
      301 ctatagattt taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca
      361 aaaatcgctc aaacctgaac taatatgatg agttaagcat tatctgcaat aattgccact
      421 cagctctaca aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg
      481 ccaacgaaga ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact
      541 gatgtaaatg accggaatga atgagagagg cagcaccgcc cctctccata agctactctt
      601 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
      661 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
      721 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg
      781 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
      841 ttccgccgag gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
      901 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg
      961 gcatggtggt gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac
     1021 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
     1081 aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
     1141 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
     1201 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
     1261 tcatcgccat cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc
     1321 gaatggacgc ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct
     1381 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
     1441 ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaact
     1501 cttcttctgt ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg
     1561 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     1621 cgatacctgc atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca
     1681 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa
     1741 acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac
     1801 actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg
     1861 gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     1921 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     1981 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     2041 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     2101 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     2161 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct
     2221 tcgaacagga gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     2281 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata
     2341 cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     2401 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     2461 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     2521 ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     2581 gtcatcttgt tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta
     2641 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     2701 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     2761 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     2821 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     2881 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     2941 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3001 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3061 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3121 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3181 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3241 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3301 cctctgcgaa cgcttcatcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3361 tatgaaaact cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca
     3421 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3481 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3541 ggcaaggtat gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat
     3601 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     3661 aaagagcgca gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     3721 agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     3781 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt
     3841 taccagataa tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc
     3901 gctattatga gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta
     3961 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     4021 cgcccgggca acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg
     4081 aatatagtct gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg
     4141 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     4201 aatcaggcct tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc
     4261 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag
     4321 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     4381 catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg
     4441 gagcccggct cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg
     4501 gactgactcc gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac
     4561 atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc
     4621 accattttcc agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt
     4681 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     4741 acccggttct gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg
     4801 cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt
     4861 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     4921 ttcctccaac cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg
     4981 ctcgcctttg gtatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt
     5041 ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc
     5101 gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc
     5161 tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     5221 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     5281 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat
     5341 tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca
     5401 gacaagcaaa gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta
     5461 tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat
     5521 tgcagcaagg gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg
     5581 tagacaaagg tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta
     5641 tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg
     5701 tttgcgaagg cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc
     5761 aaaccgataa attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg
     5821 taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg
     5881 ctgaacaagt tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg
     5941 ctggcggtcc gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc
     6001 tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg
     6061 ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc
     6121 caaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg
     6181 atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg
     6241 cctcacggga ggtttatagc caggcgcgcg cagcgggtca cggtcgccag gactggtccg
     6301 ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac
     6361 tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc gcgcatcggg
     6421 cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc
     6481 tggggcaccc gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa
     6541 ggccctctcg ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt
     6601 ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat
     6661 tcctggcaac ggtattccgg tcgatagcgt ggttattgac cgcaaaatca atccgctaca
     6721 gatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga
     6781 tgcgcagcaa cgtctggata tggtgaaaga gttcaacgaa ctgtgccact cacacggtct
     6841 ggtaagcatc attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga
     6901 acaagcgatc atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt
     6961 tgaaatgccg ctttatggca aaggtccgca acaagagctt ctctgtgctt cacaacgtct
     7021 gaatgaccat atcaatatgc catgggtgat cctctcttcc agtgtcgacg aaaaactgtt
     7081 cccgcgtgcc gtacgcgtgg caatgacggc gggcgcatcg ggattcctgg caggccgtgc
     7141 agtctgggca tcggtcgtcg gtttaccaga caacgagctg atgctccgtg acgtttgcgc
     7201 accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga
     7261 tgaggataaa aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg
     7321 aaaccgaccg catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt
     7381 taggcaataa agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta
     7441 tgttgcacgt ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg
     7501 atcacggtat taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg
     7561 cctgcgtgaa tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg
     7621 ctctgctggg tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg
     7681 attacaccat tgagattatc gagaaatatt tctggagcga agaagagcag atgtgcctgg
     7741 agtcctggga cgaagccttc agcaaaaccg aagagtaccg cggcggcaat gccaatatgc
     7801 acgcggtgga agctttcttg attgtttatg acgtcactca cgacaaaaaa tggctggatc
     7861 gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc cagaaataat cattatcgcg
     7921 ttaacgaaca cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg
     7981 cgcatcgctt ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa
     8041 tgctgcacat ccatgctgcc ctggaagccc gttgcgaaca accaccagca tggctgctag
     8101 aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg
     8161 acgggattgt ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt
     8221 ggcctatcgt cgaagcaatg ggtacggcct acgcgctcta caccgtcact ggcgatcgcc
     8281 agtatgaaac ctggtatcaa acatggtggg agtactgcat taagtacctg atggactatg
     8341 aaaatggttc ctggtggcag gagctggatg cggacaataa ggtcatcacc aaagtctggg
     8401 acggcaaaca ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag
     8461 cgcccggcat ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga
     8521 tctgaaaata gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg
     8581 accgtccggt gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat
     8641 taaggtgcct tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc
     8701 cgcgggggat tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt
     8761 tcagggttgt catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg
     8821 taaagtgctt attccgtggc cgaaccggat cgccaacggc tgttaccgtt atcaggggca
     8881 ggaatatcag ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc
     8941 ctggcgagac tggcaaatta gtgaattaac caccaccagc gttaccctga cagcttttct
     9001 gccgcccagt tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc
     9061 acacacgggt ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta
     9121 tggcgtgggt attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt
     9181 ccagcttccg gcaaactagg tttatgccgt cgatgaacac gtaaatccga caacgcttca
     9241 ccacgttgat gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat
     9301 cgaccacacc tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca
     9361 ggcgctttcg gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa
     9421 attacagcgt cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc
     9481 aggtatcgac ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta
     9541 cggacagaga aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc
     9601 gctaaaggca acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa
     9661 agtttttata tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc
     9721 aacaatctcg ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt
     9781 agattttcaa tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat
     9841 tttaacccat aacaaagata atccttgttt atggattggc tcaggtatag cggatatcga
     9901 tatgttccgc ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
     9961 cgccatcgtc agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat
    10021 tagcgccacg ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa
    10081 cgacaacctt aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat
    10141 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg
    10201 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
    10261 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
    10321 cacgcagaag tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc
    10381 cccggaatac catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc
    10441 tgacacatac atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact
    10501 gcccgactgg atttatgacg gggtaacgct cggcattcag ggcgggacgg aagtgtgcca
    10561 gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga
    10621 ctggtctggg atccgtataa cctcttttgg caagcgcgtg atgtggaact ggaagtggaa
    10681 cagcgaaaac tacccgcaac tggattcacg cattaagcag tggtatcagg agggcgtgca
    10741 gttcctggcc tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc
    10801 acaacacggc tatctagcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga
    10861 gttttacggc ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt
    10921 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
    10981 tctgcccacc gacacgtacc tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg
    11041 gcctgcgctg tgggcgaagt gtaactacga agcccttgaa gaaacgggca agctcggcga
    11101 gatcctcttc tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg
    11161 ggcgggcgac cagaacgtgg actggagtct cgacgatggc ctggcgtccg tagtcccggc
    11221 ggcgctgtcg ctggcaatga ccggacatgg tctgcatcac agcgacattg gcggttacac
    11281 caccctgttt gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc
    11341 cttcacgccg atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga
    11401 cggcgatgcg gaaactatcg cccattttgc ccgtatgact accgttttca ccaccctgaa
    11461 accttatctg aaagaggccg tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc
    11521 gctgttcctg cattacgaag acgatgcaca tacctattcc ctgaaatatc agtacctgtt
    11581 aggccgcgac attctggtcg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta
    11641 tctgccggag gataactggg tacatgcctg gacgggtgaa gcgttccggg gcggggaagt
    11701 taccgttaat gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc
    11761 ggcactgttc gcgtcgttaa acagcatcta atcacatccg cccgtgggga aactcatggg
    11821 caaagggaga acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc
    11881 ctttaaagag aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat
    11941 cggtacgctt tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg
    12001 cggcattatc tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat
    12061 tatgttggat tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta
    12121 tgcgtcattc ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt
    12181 caccggtaaa acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat
    12241 gatgaactgc tcctacggcg caatggtccc ggcaatcacc aaaaacccca acgaacgcgc
    12301 ctcactggcg gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg
    12361 attcgtgcca gttatgaatc ttatcgaagg taatcagaaa cttggctata tcttcgccgc
    12421 cacgctgttt tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga
    12481 gcgttatgtc gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt
    12541 ccgcgcaatt gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt
    12601 aggggcgttt aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga
    12661 tcccatcctg ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
    12721 cctgatgcct ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct
    12781 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt
    12841 ggcgttctcc tgcctggcgt tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca
    12901 ccgtcgagta cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct
    12961 tcaccttctt tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga
    13021 cgcaaattgg ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc
    13081 agttgatctt catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct
    13141 tctacagcct gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac
    13201 gcacggcgta ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg
    13261 accataatcc actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg
    13321 acgtcggttc gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg
    13381 atgaattagg gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta
    13441 ccgcgtttac cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta
    13501 aaggcaaatt tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc
    13561 tccagtttat cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accacgctgt
    13621 ttatgatgtt tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg
    13681 caattaccaa aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa
    13741 ccatagggct attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact
    13801 caaccgtcgg ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga
    13861 tgctgtgcta cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag
    13921 ccagtattct caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca
    13981 ttgctaacct gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca
    14041 cccagtatgt gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat
    14101 gcatcctcgt cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg
    14161 tttatttagc tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa
    14221 gtaactcttt cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa
    14281 acagcctgaa ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta
    14341 ttcgtgccga aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac
    14401 ttgctggctt cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc
    14461 aaagcgatgc gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg
    14521 caattattgc tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat
    14581 tgattattga ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg
    14641 cttccgtaac gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc
    14701 ctacgtttaa aaaatggact tattatgaaa aagattaatg caataatttt attatcttct
    14761 ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa acagggaggc atataatctt
    14821 gcctccgatc agggcgaggt tatgctacgt gtgggttata acttcgatat gggcgcgggt
    14881 attatgttaa ccaataccta cacctttcag cgagaagatg aactaaaaca tggctataat
    14941 gaaattgaag gttggtatcc tttattcaaa ccaaccgata aattaaccat ccagcccggt
    15001 ggcttaatta atgataagag tatcagttcg ggtggtgcag tgtatctgga cg
//
LOCUS       DAEEDS010000089        15033 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEEDS010000089 DAEEDS010000000
VERSION     DAEEDS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15033
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.089"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..239
                     /locus_tag="KLH32_003177"
     CDS             <1..239
                     /locus_tag="KLH32_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBE0139910.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="KLH32_003178"
     CDS             249..887
                     /locus_tag="KLH32_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBE0139911.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="KLH32_003179"
     CDS             890..2053
                     /locus_tag="KLH32_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE0139912.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..3762
                     /locus_tag="KLH32_003180"
     CDS             2137..3762
                     /locus_tag="KLH32_003180"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBE0139913.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="KLH32_003181"
     CDS             complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="KLH32_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBE0139914.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="KLH32_003182"
     CDS             complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="KLH32_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBE0139915.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4814..5563
                     /gene="yjfP"
                     /locus_tag="KLH32_003183"
     CDS             4814..5563
                     /gene="yjfP"
                     /locus_tag="KLH32_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0139916.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="KLH32_003184"
     CDS             complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="KLH32_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBE0139917.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="KLH32_003185"
     CDS             complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="KLH32_003185"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBE0139918.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7842..9239
                     /gene="ulaA"
                     /locus_tag="KLH32_003186"
     CDS             7842..9239
                     /gene="ulaA"
                     /locus_tag="KLH32_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBE0139919.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9255..9560
                     /gene="ulaB"
                     /locus_tag="KLH32_003187"
     CDS             9255..9560
                     /gene="ulaB"
                     /locus_tag="KLH32_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBE0139920.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9570..10034
                     /gene="ulaC"
                     /locus_tag="KLH32_003188"
     CDS             9570..10034
                     /gene="ulaC"
                     /locus_tag="KLH32_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBE0139921.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10048..10698
                     /gene="ulaD"
                     /locus_tag="KLH32_003189"
     CDS             10048..10698
                     /gene="ulaD"
                     /locus_tag="KLH32_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBE0139922.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10708..11544
                     /gene="ulaE"
                     /locus_tag="KLH32_003190"
     CDS             10708..11544
                     /gene="ulaE"
                     /locus_tag="KLH32_003190"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBE0139923.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSRERRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11544..12230
                     /gene="ulaF"
                     /locus_tag="KLH32_003191"
     CDS             11544..12230
                     /gene="ulaF"
                     /locus_tag="KLH32_003191"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBE0139924.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12359..12634)
                     /locus_tag="KLH32_003192"
     CDS             complement(12359..12634)
                     /locus_tag="KLH32_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0139925.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12961..13356
                     /gene="rpsF"
                     /locus_tag="KLH32_003193"
     CDS             12961..13356
                     /gene="rpsF"
                     /locus_tag="KLH32_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBE0139926.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13363..13677
                     /gene="priB"
                     /locus_tag="KLH32_003194"
     CDS             13363..13677
                     /gene="priB"
                     /locus_tag="KLH32_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBE0139927.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13682..13909
                     /gene="rpsR"
                     /locus_tag="KLH32_003195"
     CDS             13682..13909
                     /gene="rpsR"
                     /locus_tag="KLH32_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBE0139928.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13951..14400
                     /gene="rplI"
                     /locus_tag="KLH32_003196"
     CDS             13951..14400
                     /gene="rplI"
                     /locus_tag="KLH32_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBE0139929.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14471..>15033)
                     /locus_tag="KLH32_003197"
     CDS             complement(14471..>15033)
                     /locus_tag="KLH32_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBE0139930.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3511 a         3667 c         4051 g         3804 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac cgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc
     3421 aatattatgt gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta
     3481 ttgtcggaag catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt
     3541 cgtttacagc agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag
     3601 ctattcctgc tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag
     3661 gcgtggtgtc aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag
     3721 aatgatttat tgctgcgggc gacgggggga gtgtgtgtgt aagcgtatac gactgatgcg
     3781 acgctggttt cgattaacta aatgaaatat gtgaaaattg taggccggac aaggcgctcg
     3841 cgccgcatcc ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt
     3901 acgcgccagt tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca
     3961 ccttgttcat cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata
     4021 ttgctaactg agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat
     4081 tctgcagact gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca
     4141 agtaattgtt tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat
     4201 cgcggtaata aacgcccaag cttccagccg gtaatttatt gtgcacaggt aaacgcatag
     4261 ggacgcaaag caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat
     4321 ggcttgtgaa taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata
     4381 atcagctttt gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc
     4441 ccgaaccatc gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat
     4501 ctcttgcgga tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca
     4561 ggcacttaac atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt
     4621 cctgctaacc atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt
     4681 tacctggtac gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt
     4741 atgaactaat accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa
     4801 aaaggagaga cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca
     4861 tgcttatcct gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt
     4921 tacttcatcc agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg
     4981 ggtgatcatg ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg
     5041 gttaaatcaa ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc
     5101 ggcaatagcc gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat
     5161 gggcgcgatg acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag
     5221 catgatggga tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc
     5281 tgaaacggca gcacagcaga atgaattcaa taaaattgtc gcgccgctgg cagagtggga
     5341 agcgacaaac cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga
     5401 cgatgacgtt gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg
     5461 acgggataaa ctgctaacct gttcatggca gccaggcgtg cgtcaccgta ttacgcctga
     5521 ggcgttagat gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac
     5581 cccttgcgct tccagttgtt gcaggatttc cgggttagca tttttgccgg tgatcagcat
     5641 atcgatttga tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc
     5701 aaccagtacc accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag
     5761 catatcggtt ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg
     5821 cccggcatag agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt
     5881 gtactgtcca cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag
     5941 cggtagataa ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa
     6001 cgcggtggag ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc
     6061 tttagcaata cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca
     6121 gcgcgggcgc tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgct
     6181 ttcgccaagt ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac
     6241 gactttctca acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct
     6301 ttgtgcttca gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat
     6361 agccaggaaa tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca
     6421 gattacagga atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg
     6481 cgcggatagt ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc
     6541 tgccagataa acggcttgaa gccatacttc agacgatctt ttttgatctc ccacagcacg
     6601 cggatctctt gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc
     6661 actttcgcat tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta
     6721 ataccgcgcg ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg
     6781 tgcttcgcat aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg
     6841 gttttgaaca ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct
     6901 ttttgatcgg caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt
     6961 tcaatgtctt tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt
     7021 acgccccagc caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca
     7081 gcacagttct gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga
     7141 gtcgccagta ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg
     7201 cgtaggtttg gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc
     7261 attaacgggt taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg
     7321 gtgccacctt ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg
     7381 ccaggggcga cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa
     7441 gtgctcagga tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt
     7501 catgatagtt caattcgaat caacatgtga ttggttttga ttaatcctga cactattcct
     7561 tcaggaaggc aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt
     7621 gtggaaagat ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg
     7681 tttcgttgtg tgaatcacac tctatccgtg tggaattatt tgcgggtcgc gccacattta
     7741 atcataaata atcttgttgt gattactttt gaaaattaga gtgagtacac aacattccgg
     7801 gtgtgcggaa tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca
     7861 tctttaccgt gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga
     7921 cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta
     7981 aaaccataat tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca
     8041 aaccggtggt ggcgaaaatg tccgaagtct acggcattaa cggcgcaatt tccgatactt
     8101 acgcgtcgat gatggcaacc atcgaccgca tgggcgatgc ctatagctgg gtaggttacg
     8161 cggtgttgtt agcgctggcg ctgaacatct gttacgtgct gctgcgtcgc attaccggta
     8221 ttcgtaccat catgctgact ggtcatatca tgttccagca ggcggggctg attgccgtta
     8281 cgttgtttat tttcggctac tccatgtgga caaccattat ctgcacggcg attctggttt
     8341 cgctctactg gggcattacc tccaacatga tgtacaagcc gactcaggaa gtgacggacg
     8401 gctgcggttt ctccatcggt caccagcaac agtttgcatc attaattgcc tataaagttg
     8461 cgccgttcct cggcaaaaaa gaggaaagcg ttgaagacct caaactgcca ggctggctga
     8521 atattttcca cgacaacatc gtctccacgg cgattgtcat gaccatcttc tttggtgcca
     8581 ttctgctctc cttcggtatc gacaccgtgc aggcgatggc aggcaaagtg cactggacgg
     8641 tctatatcct gcaaaccggt ttttccttcg cggtggcgat cttcatcatc actcagggtg
     8701 tgcgcatgtt tgtggcggaa ctctctgaag cgtttaacgg tatctcccag cgcctgatcc
     8761 ctggtgcggt tctggcgatt gactgtgcgg ctatttatag cttcgctccg aacgccgtgg
     8821 tctggggctt tatgtggggc accatcggtc agctgattgc ggttggcatc ctggtcgcct
     8881 gcggttcctc gatcctgatt atcccaggct ttatcccgat gttcttctcc aacgccacca
     8941 tcggcgtgtt cgctaaccac ttcggcggct ggcgtgcggc gctgaagatt tgtctggtga
     9001 tggggatgat cgaaatcttt ggttgcgtct gggtggtgaa acttaccggt atgagtgcct
     9061 ggatggggat ggcggactgg tcgattctgg caccgccgat gatgcagggc ttcttctcca
     9121 tcggtatcgc ctttatggcc gtcatcattg taattgcact ggcttatatg ttcttcgctg
     9181 gccgcgcact gcgcgcagaa gaagatgcag aaaaacaatt ggcagaacag tctgcttaat
     9241 aaggagtttt gattatgacc gtacgtattc tggctgtgtg tggcaacgga caaggcagtt
     9301 ccatgatcat gaagatgaaa gtggaccagt ttttaaccca atcaaacatt gaccacacgg
     9361 taaatagctg cgcggttggc gaatacaaaa gcgagttgag cggcgcggat atcatcatcg
     9421 cttctacgca cattgcgggc gaaatcaccg tgaccggcaa caaatacgtg gttggcgtgc
     9481 gcaacatgct ctctcctgcc gactttggcc cgaaactgct ggaagtgatc aaagcgcatt
     9541 tcccgcagga tgtgaagtaa ggacgcgcca tgaaattaca tgattcgctg gcggagaata
     9601 aatccatccg cctgcaggct gaagctgaga catggcagga cgcagtaaaa atcggcgttg
     9661 acctgctggt ggcggcggat gttgtcgagc cgcgttacta ccaggcgatt ctggatgcgg
     9721 ttgaacagca cggtccgtac tttgtgctcg caccaggcct ggcaatgccg cacggtcgcc
     9781 cggaagaggg cgttaagaaa accggttttg cgctggtgac gctgaaaaag ccgctggagt
     9841 tcaaccacga agataacgac ccggtggata tcctcatcac catggcggcg gtcgatgcca
     9901 acacgcacca ggaagtgggc atcatgcaga tcgtcaacct gtttgaagat gaagaaaatt
     9961 tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact ggatctcatt gaccgcacca
    10021 acgcggcagc ttaagaagga attgaagatg tcattaccga tgttgcaagt cgcgctggac
    10081 aaccagacta tggatagcgc ctacgaaacc actcgcctga ttgccgaaga agtcgacatt
    10141 atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc gtgcggttcg cgatctgaaa
    10201 gcgctctacc cgcacaaaat cgtgctggca gacgccaaaa ttgccgatgc gggcaaaatt
    10261 ctttctcgta tgtgcttcga agccaacgct gactgggtga ccgtgatttg ttgtgcggat
    10321 atcaacaccg ccaaaggtgc gctggacgta gcaaaagagt ttaacggcga cgtgcagatc
    10381 gaactgaccg gttactggac ctgggaacag gcgcaacagt ggcgcgacgc aggtattcag
    10441 caggtggttt atcaccgcag ccgtgacgcg caggccgctg gcgtggcgtg gggtgaagcc
    10501 gatatcactg cgatcaaacg tctttccgat atgggcttca aagtcaccgt caccggtggc
    10561 ctggcgctgg aagatctgcc gctgttcaaa ggcattccaa ttcatgtgtt tatcgccggt
    10621 cgcagcatcc gtgatgccgc ttctccggtt gaagccgcgc gtcagttcaa acgttccatc
    10681 gccgaactgt ggggctaagg agcggatatg ttgtccaaac aaatcccgct tggcatctat
    10741 gaaaaagcgc tccccgccgg ggagtgctgg ctggaacgcc tgcgactggc aaaaacgttg
    10801 ggcttcgatt ttgtcgagat gtcggtagat gaaactaacg agcgcctgtc gcgcctcgac
    10861 tggagccgcg aacggcgtct ggcgctggtg aatgctattg ttgaaaccgg cgtgcgtgtg
    10921 ccgtccatgt gcctttctgc ccatcgtcgt ttcccgttgg gcagcgaaga tgacgcggtg
    10981 cgggcgcagg ggctggagat tatgcgtaaa gccatccagt tcgcccagga cgttggcatt
    11041 cgcgtgatcc agctggcggg ctatgacgtt tactatcagg aagccaataa cgaaacgcgt
    11101 cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg cgagccgcgc gcaggtgacg
    11161 ctggcgatgg agatcatgga ttatccgttg atgaactcca tcagcaaggc gctgggttac
    11221 gcgcactatc tcaacaaccc gtggttccag ctctacccgg atatcggcaa cctgtcggcg
    11281 tgggacaacg atgtgcagat ggagttgcag gccggaatcg ggcatatcgt cgcggtgcat
    11341 gtgaaagaca ccaaaccggg cgtcttcaaa aacgtgccat ttggcgaagg tgtggtggat
    11401 ttcgaacgtt gcttcgaaac gctcaaacag agtggctatt gcgggccgta cctgattgag
    11461 atgtggagcg aaacggcgga agacccggcg gcagaagtgg tgaaagcgcg catggccaaa
    11521 gcaggcatgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca
    11581 tggatctgcc gcgctatgtg ctggtgacct ttacttgggg caacgtcagc gctatcgacc
    11641 gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc atgaaagcag
    11701 acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat cgcccatctt
    11761 ccgacactgc gacgcatctc gaactctacc gtcgttaccc gtcgcttggc ggcattgtcc
    11821 atacccactc cactcatgcc accgcatggg cgcaggcggg gctggcgatc ccggcattag
    11881 gcaccacgca cgctgactac ttctttggtg atatcccgtg tacgcgtgga ttaagcgaag
    11941 aagaggtgca gggtgagtat gaactgaaca ctggcaaagt gattatcgaa acgctgggca
    12001 acgccgagcc gctgcatacg ccggaaattg tggtgtatca gcacggaccg ttcgcctggg
    12061 ggaaagatgc ccacgacgcg gtgcataacg cggtagtgat ggaagaagtg gcgaaaatgg
    12121 cgtggattgc gcgcagtatt aatccacaac tcaatcatat cgacagctac ctgatgaata
    12181 aacacttcat gcgtaagcac ggtcctaacg cttattacgg gcagaagtaa aacacgcgct
    12241 gcggaaattt ccttcctcgg gagataactg gtctaattcc gcagccgttt ttcaaaaaaa
    12301 gccccctgcg aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct
    12361 agcgataaat atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa
    12421 ttacgtggta gtacgttgcg ccttgcgcat ctgtttcttc atttaatgcc tgacgtgctt
    12481 cgctttcagt ggcgaaatta tgattgatat aaatcacgcc taagctttgc acatcgtcca
    12541 tatttctggc ctggtggtta ttaatttcaa tggctgccca tgtatttgca cttagcaaaa
    12601 gcacagccag aagggctaaa actcgactga acatagatac ctcctcgacg gctgactttg
    12661 tgtgctctcc tctgtgatga tcttctgatt taattttaat caatgataaa gaagttgatg
    12721 gtgaccattt ctgatgcagt tgttcaaaaa aacactgtga cggaatgtga tgaacttcaa
    12781 atcagcggtt tagaggttat ttgcgaaagg ggagatttat ttctgctccg cccttgagtt
    12841 tagcggggta tgccagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt
    12901 gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg
    12961 atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc
    13021 atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa
    13081 gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt
    13141 ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc
    13201 aacgatgccg ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct
    13261 ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc
    13321 gctgatgatg ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg
    13381 ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac
    13441 tgccagttcg tgcttgagca tcgttctgtg caggaggaag ccggctttca ccggcaggca
    13501 tggtgtcaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata
    13561 acggtcggca gtcgcataac cgttcagggg ttcatttcat gccacaaggc aaagaacgga
    13621 ctgagcaaaa tggttttgca tgccgagcag attgaattga tagattctgg agactagcca
    13681 tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga
    13741 gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt
    13801 cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa
    13861 acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt
    13921 ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca
    13981 aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg
    14041 gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt
    14101 cgcgctgaac tggaagctaa actggctgaa gttctggcag ctgctaatgc tcgcgctgag
    14161 aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg
    14221 ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg
    14281 gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga acacgaagtg
    14341 agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa
    14401 ttcgttattc aacgagacgt aaaaacgccg accattggtc ggcgttttgc tttctatttt
    14461 tcgtcaggca ttaatttcgc aagtagatcc attcaccatt ctcttgtgtc cataagtcag
    14521 gatggtttgc tggcgttaga tgcatacgat cactccttat gtctcggtcc tgcggcactc
    14581 gctcgaaacc taaacccgag tagattgcgg ctgaatttga taaggaacgt agaccaattt
    14641 tcaaatcatg gggtaaatca gaatacgcgt ttagcaatgc agcaatgatg aatccgaggg
    14701 cattagaaac gcctgtaaca gtgtgaagtt ggtcgatata aagatcgatt gttggtagtt
    14761 cagatgacca gcaattaggc atttgggcag gaagggttac aacgtatcct atcgtttggc
    14821 cggtatagtc tggagtacta taaccaggtc ccaaaacaac ctcgtgtatt gtgtatttac
    14881 tgttgggttc gaaggattcg acatcttgca ataaatattt cagttctgcg gttaacctcg
    14941 cacccgcgta agctttattg tatttctggg cgtgaatatt atctaatgta gcttcatggt
    15001 tccatccaaa agaattatca ccattcattg tta
//
LOCUS       DAEEDS010000090        14920 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEEDS010000090 DAEEDS010000000
VERSION     DAEEDS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.090"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="KLH32_003198"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="KLH32_003198"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBE0139931.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KLH32_003199"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KLH32_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBE0139932.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KLH32_003200"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KLH32_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBE0139933.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="KLH32_003201"
     CDS             2977..3060
                     /locus_tag="KLH32_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBE0139934.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KLH32_003202"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KLH32_003202"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBE0139935.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KLH32_003203"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KLH32_003203"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBE0139936.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="KLH32_003204"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="KLH32_003204"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBE0139937.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="KLH32_003205"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="KLH32_003205"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBE0139938.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KLH32_003206"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KLH32_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KLH32_003207"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KLH32_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBE0139939.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="KLH32_003208"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="KLH32_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBE0139940.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KLH32_003209"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KLH32_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBE0139941.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="KLH32_003210"
     CDS             13186..14385
                     /locus_tag="KLH32_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE0139942.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3713 a         3789 c         3928 g         3490 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catttttagg taatgctgcc aacttactga
    14881 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAEEDS010000091        14569 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEEDS010000091 DAEEDS010000000
VERSION     DAEEDS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.091"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..581
                     /locus_tag="KLH32_003211"
     CDS             <1..581
                     /locus_tag="KLH32_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBE0139943.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="KLH32_003212"
     CDS             complement(635..1357)
                     /locus_tag="KLH32_003212"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE0139944.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="KLH32_003213"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="KLH32_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KLH32_003214"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KLH32_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBE0139945.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KLH32_003215"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KLH32_003215"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBE0139946.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KLH32_003216"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KLH32_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBE0139947.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KLH32_003217"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KLH32_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBE0139948.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KLH32_003218"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KLH32_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBE0139949.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KLH32_003219"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KLH32_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBE0139950.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KLH32_003220"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KLH32_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBE0139951.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KLH32_003221"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KLH32_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBE0139952.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KLH32_003222"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KLH32_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBE0139953.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14569)
                     /locus_tag="KLH32_003223"
     CDS             complement(13620..>14569)
                     /locus_tag="KLH32_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0139954.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3759 a         3718 c         3318 g         3774 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atcccgaga
//
LOCUS       DAEEDS010000092        14515 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEEDS010000092 DAEEDS010000000
VERSION     DAEEDS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.092"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(113..379)
                     /locus_tag="KLH32_003224"
     CDS             complement(113..379)
                     /locus_tag="KLH32_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBE0139955.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="KLH32_003225"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="KLH32_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBE0139956.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="KLH32_003226"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="KLH32_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="KLH32_003227"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="KLH32_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBE0139957.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="KLH32_003228"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="KLH32_003228"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBE0139958.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KLH32_003229"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KLH32_003229"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBE0139959.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KLH32_003230"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KLH32_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBE0139960.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="KLH32_003231"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="KLH32_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KLH32_003232"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KLH32_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBE0139961.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="KLH32_003233"
     CDS             complement(12641..14095)
                     /locus_tag="KLH32_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBE0139962.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14515)
                     /locus_tag="KLH32_003234"
     CDS             complement(14092..>14515)
                     /locus_tag="KLH32_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0139963.1"
                     /translation="FSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVSLVPVSE
                     LWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATG
                     NFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3873 a         3663 c         3429 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg aaaac
//
LOCUS       DAEEDS010000093        14318 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEEDS010000093 DAEEDS010000000
VERSION     DAEEDS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.093"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..107)
                     /locus_tag="KLH32_003235"
     CDS             complement(<1..107)
                     /locus_tag="KLH32_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBE0139964.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGG"
     gene            complement(148..603)
                     /gene="csgB"
                     /locus_tag="KLH32_003236"
     CDS             complement(148..603)
                     /gene="csgB"
                     /locus_tag="KLH32_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBE0139965.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="KLH32_003237"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="KLH32_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBE0139966.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="KLH32_003238"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="KLH32_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBE0139967.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="KLH32_003239"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="KLH32_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBE0139968.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2868..3574
                     /locus_tag="KLH32_003240"
                     /pseudo
     CDS             2868..3574
                     /locus_tag="KLH32_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="KLH32_003241"
     CDS             complement(3707..4198)
                     /locus_tag="KLH32_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBE0139969.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="KLH32_003242"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="KLH32_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBE0139970.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="KLH32_003243"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="KLH32_003243"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBE0139971.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="KLH32_003244"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="KLH32_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6843..6930
                     /locus_tag="KLH32_003245"
     tRNA            6843..6930
                     /locus_tag="KLH32_003245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6877..6879,aa:Ser,seq:gga)
     gene            complement(7080..7361)
                     /locus_tag="KLH32_003246"
                     /pseudo
     CDS             complement(7080..7361)
                     /locus_tag="KLH32_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7643..8647)
                     /locus_tag="KLH32_003247"
     CDS             complement(7643..8647)
                     /locus_tag="KLH32_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HBE0139972.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8719..9279)
                     /locus_tag="KLH32_003248"
                     /pseudo
     CDS             complement(8719..9279)
                     /locus_tag="KLH32_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9364..9561)
                     /locus_tag="KLH32_003249"
     CDS             complement(9364..9561)
                     /locus_tag="KLH32_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139973.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9573..10064)
                     /locus_tag="KLH32_003250"
     CDS             complement(9573..10064)
                     /locus_tag="KLH32_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139974.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10061..10435)
                     /locus_tag="KLH32_003251"
     CDS             complement(10061..10435)
                     /locus_tag="KLH32_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBE0139975.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10482..10856)
                     /locus_tag="KLH32_003252"
     CDS             complement(10482..10856)
                     /locus_tag="KLH32_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBE0139976.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11019..11240)
                     /locus_tag="KLH32_003253"
     CDS             complement(11019..11240)
                     /locus_tag="KLH32_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBE0139977.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11303..11779)
                     /locus_tag="KLH32_003254"
     CDS             complement(11303..11779)
                     /locus_tag="KLH32_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBE0139978.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11795..12268)
                     /locus_tag="KLH32_003255"
     CDS             complement(11795..12268)
                     /locus_tag="KLH32_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBE0139979.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12610..13431)
                     /locus_tag="KLH32_003256"
     CDS             complement(12610..13431)
                     /locus_tag="KLH32_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBE0139980.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13531..13704)
                     /locus_tag="KLH32_003257"
     CDS             complement(13531..13704)
                     /locus_tag="KLH32_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBE0139981.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13824..14273)
                     /locus_tag="KLH32_003258"
     CDS             complement(13824..14273)
                     /locus_tag="KLH32_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0139982.1"
                     /translation="MSWSHYLSPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGD
                     ATYRAAIDKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDY
                     IVTLYNPKTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3658 a         3502 c         3425 g         3733 t
ORIGIN      
        1 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
       61 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
      121 cggattgatt taaaagtcga atggtaatta acgttgtgtc acgcgaattg ccatttgcga
      181 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
      241 accagaacct ttctgtataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
      301 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
      361 ctttgcccga ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
      421 ccgtaactga gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga
      481 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
      541 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
      601 catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg
      661 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
      721 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtattaaa
      781 acccgcgtaa atacgctgtt atctacgcaa aaaatatttt gttttctctt aaatctccgt
      841 tttccgctat caaaaatcac cagacagtca ttcttcttgc ccgtcgctga ttgctgcgat
      901 atgtcttgcg cacaagccgt gacaaacctc gtctttttgc agtaaaaaaa ttgtccacgg
      961 aggtgtggag aaaaaacaaa aacgttttac atgacgaaag gactacaccg aaatattttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagtggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acggacgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gtaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctag cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg agatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccac gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaatgtttga ataaacgcgc gattttttca aaaagtttgt
     6781 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
     6841 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
     6901 tcgggggttc gaatcccccc ctcaccgcca gattataagt gcatgttatt gtttaacatg
     6961 cgctttttta tttttccttc cagacagtat gccatcgggt atgccatctg aaaccggttt
     7021 atatgccccg cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
     7081 tatactgcca gcatatcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
     7141 tctggttttc tgaacgtgat atgcgcggag cccttcttaa agtatctgat gctgaacatc
     7201 tcatcttcat agctctcttt accctgaaca gactgaatgt gatcatccag acgaagggtg
     7261 atgtttcccc ggttgtcggg gattggttta ccgctgaaca ggtgcaacat tctttccaga
     7321 tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa atctatggtc acccccgttt
     7381 ttgcaacact gatttttgat taatggttgg cttgcttaaa tctatccggc gtctctgtgg
     7441 gcaaggatgc ccgcgccccg atgagttccg cacctgataa tcctaaaaat cccctcggct
     7501 tcagtgagcc atttcatctg ttaggttttt aatcaggtcg atttgccgtt catgtcatca
     7561 ataatcagtc ttcgcaaaac cagaaaggta acttgtatct tattgtttaa aataaactaa
     7621 ttgagttata tcagcatcta tatcaggcct gatattcttt tttctgagta gtgattgccc
     7681 aggcaattcg agccagttta tttgccagag cgcaggtaac cacattcgaa tgcttcttgt
     7741 tgagttgctc ccgaacccac tcagcaagtt ttccatgctt gcgttcaagt cgcattatga
     7801 atgagcgggc acactgaacc agtaaccggc gtaaattttt atcacctcgc ttactgatac
     7861 ccaacagagt cgattttcct ccagtgctat actgccgggg aacaagtcct gtggaagctg
     7921 caaaatccct gctgcatgag aactgtttcc cgtcaccaag ttgcgacgat agtaagctgg
     7981 cagtaatcgg accaacacca ggaattgtca taatgcgctg agcggtatca tcagcgttga
     8041 ttgactgact taattctgat tccagctctg ctatttgctc aacaaggtaa aagtagtggg
     8101 tatgcaacct caccaaaagg cggctcagat attcggggat ttcgtgttca gccaacacga
     8161 gagacaaccg ttttattaca gcatcaccta tcggcaggct gattccaaat tccagcaaga
     8221 aaccgtggat ctggttggtt gttttcacct tatctctgat gagcgattct ctgacccggt
     8281 gtaacgccct catggcctgc tgggtctcag ttcttggctg gacaaaacgc attgatggtc
     8341 gtgaagccgc ttcgcatatg gcttctgcat caacaaaatc attcttatta cttttgacaa
     8401 acgggcgaac gaactgtgga gaaatcagct tagtttcatg cccaaaatca gagatacagc
     8461 gagccataaa gtgtgcaccc gcacaggctt ccattacaac aactgagggc ggacatgtcg
     8521 ccagaaactg catcagttta gtacgggtaa attttttacg cagaagagcc ttaccagact
     8581 tgtcttgaca atgaatgtgg aaggagtgct ttccgagatc gataccaata agtgtgatgt
     8641 tctgcatgat ggtctctccg ttaaagggat ttaccttaac agcgtactgc tgttaaggtg
     8701 ggggtgacca tatcattatc cccaccggtc ccatctcacc agattgttga cgataatttt
     8761 actgccaaat ttacaggggc aattggtttt gtaattccag ttcagccctc tgaagacgtt
     8821 gatcacccct cgctcaaaca cctcatcctt attctggtgc agttgttcaa atgtgctcag
     8881 aatgttcgct tcgctgattt ccggaaaatt atcgtactcc agtgaccggt accatgtatc
     8941 acgggcctgc gtatccatta atgacagcat cccggaacgt tgcatcaggt cacgccagat
     9001 ttcgcgatca agattccggg taatggcttt cattgctgtt tcgggtttct ccatcagcca
     9061 gcagccgcag cgaaaatcct gtttcatggc ccagtccggg gcagttttac ctccgatgct
     9121 ttccgtcagc accgagattt cagccagttg tcggataagt gtgtcgattt gcaacagtgt
     9181 actctttcgt acagccagaa tgtggcgaat gctggttgag cagataacgt ccgtatgccc
     9241 gttgaccact tccggacggg agattgtgat atctgacata atcgatattc ctttttgcag
     9301 ataaatgaaa acgccagccc tgtgcaggct ggcgtcagag gaatgaagtc tggtgagtga
     9361 gcttcaccgg ttctggtgca gaagctgcag atgacgcagg gtacggacat cctcccgggc
     9421 ctgttcgata cagggcaaca gtgctgctga atccgtttta tcctcatcgt tgtcaaagat
     9481 aattcccgat ccgcagtcga tattgtcctg cagccacgca atcagaatat ccagcgctgt
     9541 ttccgtggtt aatgatttca tgttgttcct ggttaccagt cgaaagtggg taaacccggc
     9601 aggcattcag cgctggcatc cagatgaatg agactgacgc cataacgccg gatgagtgtg
     9661 acgaccagac gacggaacgt ttttgacagc cccaggcgtt taagccgcag aaccgggtat
     9721 gaccacgcat cggtacgtaa cagatatccg ctacctgtaa aatgaatcca ttctgattca
     9781 ccgaagtcac tggtctggtg tgacagcgag tacaaccagg cgttgtccgt ttccgtgata
     9841 tgtgcggtac tgcagcgtat gccggtaagg gtagcaaatg gtggcgtgca ttctgctgag
     9901 gtctggtcag attcatcaac aacacgaagg gagtagccgt tatcgcagac catgttaatc
     9961 agttcagcga gctcaatacc atcgacgtca acgacaatgc gccctatgtt cagtgcctgt
    10021 acgttaacgc tgtcggcttc cagtgtcagg gccagtttca tcatttcgcc tccggatact
    10081 tacccagggt aatgttattt accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc
    10141 ggcgagcccg gaggatatca atactgttta ttaactgaga gcggggacag gtgctgaatc
    10201 ccggctggtc ggtacgcacc agcgcgtatt tttccacgag aaaattaact gcatcacaca
    10261 gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc atcgacaaac ggtgtgtcat
    10321 tcagtgtgag gccataatgc tggtccagca gtcggatgag cagtgtctgc cagatggtga
    10381 cgggagacgg gcagcgagat acctcccgta catgtgtgtc ggataatgtt ttcatcttaa
    10441 gttctctgta actggtagtg ctaaaggtga gagcttcggg gttatgaggt ggttgcgggt
    10501 gctggtgtcg gataaacagc cagataaacg taaccacagg agccgagggt gtcggcttcg
    10561 caggtcagcc cttttgcata cagcgtgacg gtatgctgat atcggggatt cagttcaccg
    10621 ctggtgagca tgagttccag ttgtttcatc agcagcggaa aggcctggtc cagatggtat
    10681 gcatctgcgt cgctgaagcg gcctctgata ccggcgcggt cggcaaggta atacaggcgg
    10741 ttaccctctt gcaccagacg agcgccaaaa cagggcctca ctgtgcaggg aagcccccac
    10801 caggggcggt cgtgattgtc atcgggatga gttatcccgg agagtttgtc tgacacgata
    10861 aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg acaggtcagc cggcgcgctg
    10921 accggtttcg gtgaagatgt atgcattgat gagaatggcc tcatccacag cggcgtctga
    10981 ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc agttcagggt gacactcatc
    11041 agccggacat acgggccaaa actgtcctta cggcgttctg caaacacggc aagaacgccg
    11101 ggaatatcct gcacttcacg accagtatac gcttcagcac tgccgtgcca gcgatactta
    11161 ccggtacaga acggaaaaag acgggatgca ggatgctgtt ggtgaatacg catggcttca
    11221 ccacgggtga tgattctcat gatgtgatac ctctgaagac agaagataaa agtgaaaaca
    11281 ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggt
    11341 tgccaccgac tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca
    11401 gacgttcggt gataagccgg tctgccttac tgggtgtgac ttcaccggac gggtgattgt
    11461 gcgccagcac cacggcagcg gcattgtggt acagggcgcg tttaatcact tcccggggat
    11521 gaacttccgt acggttgatg gtgccggtga agagggtttc accggcaatc agctgattct
    11581 ggttgttcag atacagcacc cggaactctt cccgctccag tcccgccatg ttcagaatca
    11641 gccattcccg tgccgcacgg gtggaggtga aggccacgcc gggttcatga agatggcggt
    11701 ccagggtttg cagggcccgc agaatgagac tgcgctcgcc gggcgtcatg tttccgggca
    11761 gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc gatgatgcgc ataatggcgc
    11821 tgcattccgg atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat
    11881 aacactccgt acgacaggca tgatgactgt acgtcatcag acaggcagca atgccggcgg
    11941 cttccgggct catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttcgt
    12001 catcatcgtc atccggttcg ggtgccataa atgccccgcc gttgttcagg gtgtacagat
    12061 tccagatacc accgcagtag tcttcacaca gacggtccat ccagccgaag acacggggtt
    12121 ccagggtgac ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt
    12181 catcagggac gggagtggca accagttgtt gcaggccagt ctccggtgca caggccgaag
    12241 cggtggtgtt ctgagacaat gttttcatgt atgaaatcct tgtaatgtga ataaatgaga
    12301 gggagtaaag gtcgggagct ggcaacatca cgttcatcac tgaccgtgat gacccggtaa
    12361 tatcagggat gaatgatatt cggctgaaat tccggacagc cgtacgcgta accgttccgg
    12421 tcacagacgg atggccagat gccattgcct gtatactcag tctgcacaga ctgatttctc
    12481 cgggacggta aagcagacgg gtacaccagg cgcacaggtc atcatcagaa atggaacgac
    12541 accccggcag cgcctccggg gcgtcgggtg attctggtgc ctgaatcacg ccttcaggga
    12601 catgctgcct cagcgcaggc tctccagcag cgtttctgcc attacccaca atgcgcggtt
    12661 gagcttaata tcggtgtcga tgttgtgaat ggcacgggtg tggatacgtt ttccttttgc
    12721 actgcggccg gaaatcccgc ctttcagcat attctcctgg atggtctgat atgcgctcca
    12781 caggtcttta ccgtaatcct ccctgcgtcg tggtgtcaga atgtcagcgg tggtgactgg
    12841 ctgatgttcg tcaccataac ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg
    12901 tggcggcagg accagcgact gcatggcatc acgcttctcc tctatccggt caaacacgcc
    12961 caccacctcg taagcccctt caataacttt ctccaccaca tttccccggt gtggaacacg
    13021 caattctccc agagactgac cgcagaccag tccattggta caaatggcac gaaaatatcc
    13081 gggtaacatc tggtagctgg atgaaccgtc atgagagttg agcagaataa tttcagggac
    13141 atgctgaccg gttatctgtc cggcccgccg cagacgcagc atgtgttttg tgtattcccg
    13201 gcgacccggg tcacgcacac gtgtctggca ggcgaagaat ggctgaaagc cttcccgctg
    13261 caggctttcc agtacggtga tggtgggagt gtacgcatag cgtttactgc gggaagtatg
    13321 ccggtcttca ccgaaaatac tgggaacata gtgcatcagt tcttcgtgtg tcagcggacg
    13381 gtcacggcgt atctggtttg cagcataacc aaaacgactg gctagtcgca taatttgctc
    13441 cttatcggtg gttaagattt actggtgtaa taaatgaaaa agccacgtct cccggagaag
    13501 acgcggcctg atggacgaaa ttaatgaggt ttattgtctg agaagaccgt cactggcgaa
    13561 ccttccggcg tcagtctctg cgttgcggat aacccgacgg aaatgagttc cctggtcatc
    13621 gtcgataaac acattgcggt aagctgttgt gatgacaaca gcctgctcgc gtgagaaagg
    13681 gacttcgggc aataaacgtg acatacaacc agacctgtat ttggttccct gaaaatataa
    13741 gtccctgtca tcatggcaga gccgtattta cttttgatat caataaatta ataacgcgaa
    13801 gatgaaccgc ataacttacg cctttaattt gttttgttta cgtagtccct gaccaggtac
    13861 gcgaattcgt gaacgccagt ttcaccaaac caaatcaggt aaccagggtc gatgttgtaa
    13921 tagtaaccaa cgactttatg cgtttttggg ttatagagtg ttacgatata gtcccagccg
    13981 caggcgtggg ctttacaacc gccaccttct acccaggtcg ttccgttaat gtttacagga
    14041 tacgcaccac catcaggacc atccatatag ccgtcttccc caaacattcc gtttaaccca
    14101 acagggttta atgctttgtc gatagcagct ctgtaggtag cgtcaccttc gtataactcg
    14161 tacgttgttg gacatgcacc aggctggtca gcccgggctt ttgagtcgca tttaagtgtc
    14221 tgatttacag tggcggcgtt tgtgtcagga cttaggtaat gactccaact tattgatagt
    14281 gttttatgtt cagataatgc ccgatgactt tgtcatgc
//
LOCUS       DAEEDS010000094        14118 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEEDS010000094 DAEEDS010000000
VERSION     DAEEDS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.094"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(197..2872)
                     /gene="adhE"
                     /locus_tag="KLH32_003259"
                     /gene_synonym="adhC"
     CDS             complement(197..2872)
                     /gene="adhE"
                     /locus_tag="KLH32_003259"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBE0139983.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3349..3996
                     /gene="ychE"
                     /locus_tag="KLH32_003260"
     CDS             3349..3996
                     /gene="ychE"
                     /locus_tag="KLH32_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBE0139984.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            4734..6365
                     /gene="oppA"
                     /locus_tag="KLH32_003261"
     CDS             4734..6365
                     /gene="oppA"
                     /locus_tag="KLH32_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBE0139985.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6451..7371
                     /gene="oppB"
                     /locus_tag="KLH32_003262"
     CDS             6451..7371
                     /gene="oppB"
                     /locus_tag="KLH32_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBE0139986.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7386..8294
                     /gene="oppC"
                     /locus_tag="KLH32_003263"
     CDS             7386..8294
                     /gene="oppC"
                     /locus_tag="KLH32_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBE0139987.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8306..9319
                     /gene="oppD"
                     /locus_tag="KLH32_003264"
     CDS             8306..9319
                     /gene="oppD"
                     /locus_tag="KLH32_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBE0139988.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9316..10320
                     /gene="oppF"
                     /locus_tag="KLH32_003265"
     CDS             9316..10320
                     /gene="oppF"
                     /locus_tag="KLH32_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBE0139989.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..10702)
                     /locus_tag="KLH32_003266"
     CDS             complement(10373..10702)
                     /locus_tag="KLH32_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBE0139990.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10737..12197)
                     /gene="cls"
                     /locus_tag="KLH32_003267"
     CDS             complement(10737..12197)
                     /gene="cls"
                     /locus_tag="KLH32_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBE0139991.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12340..12513
                     /locus_tag="KLH32_003268"
     CDS             12340..12513
                     /locus_tag="KLH32_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBE0139992.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12568..13821)
                     /gene="kch"
                     /locus_tag="KLH32_003269"
     CDS             complement(12568..13821)
                     /gene="kch"
                     /locus_tag="KLH32_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBE0139993.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         3647 a         3285 c         3491 g         3695 t
ORIGIN      
        1 cccagagaag tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt
       61 ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact
      121 ggatcggtca actgatcctt aactgatcgg cattacccca gaaggggccg tttatattgc
      181 cagacagcgc tactgattaa gcggattttt tcgctttttt ctcagcttta gccggagcgg
      241 cttctttttt cgctgcagtt tcaccttcta cataatcacg accgtagtag gtatccagca
      301 gaatctgttt cagctcggag atcagcgggt aacgcgggtt agcgccggtg cactggtcat
      361 cgaatgcatc ttcagacagt ttatccacgt tcgccaggaa gtctgcttcc tgaacgccag
      421 cttcacggat agatttcgga atacccagtt cagctttcag cgtttccagc catgccagca
      481 gtttctcgat cttagcagca gtacggtcgc ccggtgcgct cagacccagg tggtcggcaa
      541 tttcagcata acgacggcga gcctgcggac ggtcatactg gctgaatgca gtctgcttgg
      601 tcgggttgtc gttcgcattg tagcgaataa cgttacaaat cagcagggcg tttgccagac
      661 cgtgcggaat atggaactgg gaacccagtt tgtgcgccat tgagtgacat acacccagga
      721 aggcgttcgc aaatgcgata cccgcgatag ttgctgcact gtgaacacgt tcacgcgcta
      781 ccggattttt agacccttcg tggtaggacg ctggcagata ttctttcagc agtttcagtg
      841 cctgcagagc ctgaccatca gagaactcag atgccagtac agaaacataa gcttccatgg
      901 cgtgagttac tgcgtccaga ccaccgaaag cacacaggga cttcggcatg tccataacca
      961 ggttggcgtc gacaatcgcc atatccgggg tcagcgcata gtccgccagc gggtatttct
     1021 gaccagtagc gtcgtcagtt acaaccgcaa acggagtgac ttcagaacct gtaccagaag
     1081 tggtggtgac agcgatcatt ttcgctttca cgcccatttt cgggaacttg tagatacgtt
     1141 tacggatatc cataaagcgc agcgccagct cttcgaagtg agtttccgga tgttcgtaca
     1201 taacccacat gatcttcgcg gcgtccatcg gggaaccacc acccagcgcg ataatcacgt
     1261 ctggtttgaa ggagtttgcc agttctgcac ctttacgaac gatgctcagg gtcgggtctg
     1321 cttctacttc gaagaagact tcagtttcaa cgcctgctgc tttcagtacg gaagtgatct
     1381 gatcagcata accattgttg aacaggaagc ggtcagtcac gatgagcgca cgtttgtggc
     1441 catcagtaat cacttcatcc agcgcgattg gcagggagcc acggcggaag tagatagatt
     1501 tcggaagttt gtgccacaac atgttttcag ctcgcttagc aacggttttc ttgttgatca
     1561 ggtgtttcgg accaacgttt tcagagatgg agttaccacc ccaggaacca caacccagag
     1621 tcagggaagg tgcgagtttg aagttataca ggtcaccgat accaccctga gaagccgggg
     1681 tgttaatcag gatacgagct gttttcattt tctgaccgaa gtaagaaacg cgagccggtt
     1741 ggttatcctg gtcagtgtac aggcaagagg tatgaccgat accgcccata gcaaccagtt
     1801 tctctgcttt ttctaccgcg tcttcgaaat ctttagcgcg gtacattgcc agagtcgggg
     1861 acagtttttc atgtgcgaac ggttcgcttt catcaacaac ggtcacttca ccgatcagaa
     1921 tcttggtgtt ttctggtaca gagaagcctg ccagttcagc aattttatag gctggctgac
     1981 caacgatagc cgcgttcagc gcaccatttt tcaggataac atcctgaaca gctttcagct
     2041 ctttaccctg caacagatag ccgccgtggg ttgcgaaacg ttcacgtact gcgtcataaa
     2101 cagagtcaac aacaacaaca gactgttcag aagcacagat tacgccgttg tcgaaggttt
     2161 tggacatcag tacagatgca actgcacgtt tgatatcagc agtttcatca ataacaactg
     2221 gagtgttacc cgcgcctaca ccgatagctg gtttaccgga gctgtatgcg gctttaacca
     2281 tgcccggacc accagtcgcg aggatcaggt tgatgtctgg gtggtgcatc agtgcgttag
     2341 acagttcaac agaaggttga tcgatccagc cgatcagatc tttcggagca ccggcagcga
     2401 tagcagcctg cagaacgata tcagccgctt tgttggttgc atcttttgca cgcgggtgcg
     2461 gggagaagat aatggcgtta cgggtcttca ggctgatcag cgatttgaag atagcagttg
     2521 aagtcgggtt agtggtcgga acgataccgc aaataatacc gattggttca gcgatagtga
     2581 tggtaccaaa agtgtcgtct tcagacagaa caccacaggt tttttcatct ttataggcgt
     2641 tgtagatata ttcagaagca aagtggtttt tgatcacttt atcttcgacg atacccatgc
     2701 cggattcggc aacggccatt ttcgcgagtg ggattcgagc atctgcagca gccagagcgg
     2761 cggcgcggaa gattttgtct acttgctctt gagtgaaact ggcatattca cgctgggctt
     2821 tttttacacg ctctacgagt gcgttaagtt cagcgacatt agtaacagcc ataatgctct
     2881 cctgataatg ttaaactttt ttagtaaatc atctgctcga atacgagagt atagtcagtg
     2941 cggtgatgat ttgcttaacc gatgaaaatc aaaagcttac tcgcgctcac actcactgtg
     3001 atttactaaa agagtttaaa cattagagtt attatctcta atgcgtcact tccaggtggc
     3061 gtaagcaaga ttactcactt ctgggtactg attacgtgat ccaaatcaaa tttttgcaaa
     3121 gctgacacct ttcagcatcg cttttcgcca ttatagctaa cagttaataa attgtagtat
     3181 gatttggtgg ctacattagc atgttttgca caactagata acaataacga atgatagcaa
     3241 ttttaagtag ttaggaggtg aaaaatgctg tcaaaaggcg tattgtcagc gcgtcttttc
     3301 aaccttattt atggctaaca ttatccggct tttgcttcgg agcgaaccgt gattcagacc
     3361 ctttttgatt ttcccgttta cttcaaattt ttcatcgggt tatttgcgct ggtcaacccg
     3421 gtagggatta ttcccgtctt tatcagcatg accagttatc agacagcggc agcgcgaaac
     3481 aaaactaacc ttacagccaa cctgtctgtg gccattatct tgtggatctc gctttttctc
     3541 ggcgacacga ttctacaact ttttggtata tcaattgatt cgttccgtat cgccgggggc
     3601 atcctggtgg tgacgatagc gatgtcgatg atcagcggca agcttggcga ggataaacag
     3661 aacaagcaag aaaaatcaga aaccgcggta cgtgaaagta ttggtgtggt gccattggcg
     3721 ttgccgttga tggcggggcc aggggcgatc agttctacca tcgtctgggg tacgcgttat
     3781 cacagcatta gctatctgtt tggtttcttt gtggctattg cattgttcgc tttatgttgc
     3841 tggggattgt tccgcatggc accgtggctg gtacgggttt tacgccagac cggcatcaac
     3901 gtgattacgc gtattatggg gctattgctg atggcattgg ggattgaatt tatcgttact
     3961 ggtattaagg ggattttccc cggcctgctt aattaattcc tttcaaatga aacggagctg
     4021 ccatgctccg tttacttcgt cattattttt actttgttcc cgcgcagtta tcaaaagcaa
     4081 aaggaatagg taaaaatatt cttctcaaat tacagttagt tataaggatt tccttaactg
     4141 tttctcctca ccatcatgtt attttcgcca catcataatc ctgggcttgc tgaagaataa
     4201 ttgaaatgat attagtaatt ccactgcctt tggtagagga aagtgctaaa taataatcaa
     4261 ttgttaaatt attgtgcatt tcactactgg aactgtaatc agaaaagata gacatgctta
     4321 gccaatctct atttgattga attgaaagat gtttgttaag gcatggatgc aagctataga
     4381 ttctgatacg gtcaataaaa gagaatcgct taacaatttt gcaaaatgta ttggcgagta
     4441 agaaccgcat ttggtacttt ccgggcaacc gccagacgat tctttattgg taatgagaat
     4501 aattaacaat taaagagcgt cgcgaaagaa taatgtgtct cgacagggga gacacagtac
     4561 gaatcgacat aaggtgatcg tctgaatcac cagaataaat aaagtcggtg atagtaatac
     4621 gtaacgataa agtaacctga cagcagaaag tctccgagct tgtgcagggt cccaatccgg
     4681 gattacacat gctggttaat accagtaatt ataatgaggg agtccaaaaa acaatgacca
     4741 acatcaccaa gagaagttta gtagcagctg gcgttctggc tgcgctaatg gcagggaatg
     4801 tcgcgctggc agctgatgta cccgcaggcg tcacactggc ggaaaaacaa acactggtac
     4861 gtaacaatgg ttcagaagtt cagtcattag atccgcacaa aattgaaggt gttccggagt
     4921 ctaatatcag ccgagacctg tttgaaggct tactggtcag cgatcttgac ggtcatccag
     4981 cacctggcgt cgctgaatcc tgggataata aagacgcgaa agtctggacc ttccatttgc
     5041 gtaaagatgc gaaatggtct gatggcacgc cagtcacagc acaagacttt gtgtatagct
     5101 ggcaacgttc tgttgatccg aacactgctt ctccgtatgc cagttatctg caatatgggc
     5161 atatcgccgg tattgatgaa attcttgaag ggaaaaaacc gattaccgat ctcggcgtga
     5221 aagctattga tgatcacaca ttagaagtca ccttaagtga acccgttccg tacttctata
     5281 aattacttgt tcacccatca acttcaccgg tgccaaaagc cgctatcgaa aaattcggcg
     5341 aaaaatggac ccagcctggt aatatcgtca ccaacggtgc ctatacctta aaagattggg
     5401 tcgtaaacga acgaatcgtt cttgaacgca gcccgaccta ctggaacaac gcgaaaaccg
     5461 ttattaacca ggtaacctat ttgcctattg cttctgaagt taccgatgtc aaccgctacc
     5521 gtagtggtga aatcgacatg acgtataaca acatgccgat cgaattgttc cagaagctga
     5581 aaaaagagat cccggacgaa gttcacgttg atccatacct gtgcacttac tattacgaaa
     5641 ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg taccgcactg aaactaggta
     5701 tggaccgcga tatcattgtt aataaagtga aagcgcaggg cgacatgccc gcctatggtt
     5761 atactccacc gtatactgat ggcgcaaaat tgactcagcc agaatggttt ggctggagcc
     5821 aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga agcgggttat accgcagaca
     5881 aaccgttgac catcaacctg cggtataaca cctccgatct gcataaaaag ctggcgattg
     5941 ctgcctcttc attgtggaag aaaaacattg gggtaaacgt caaactggtt aaccaggagt
     6001 ggaaaacgtt cctcgacacc cgtcaccagg gcacttttga tgtggcccgt gcaggctggt
     6061 gtgcagacta caacgaacca acttccttcc tgaacaccat gctgtcgaac agctcgatga
     6121 ataccgcgca ttataagagc ccggcctttg acagcattat ggcggaaacg ctgaaagtga
     6181 ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga acaacagctg gataaggatt
     6241 cggccattgt tcctgtttat tactacgtga atgcgcgtct ggtgaaaccg tgggttggtg
     6301 gctataccgg caaagatccg ctggataata cctatacccg gaatatgtac attgtgaagc
     6361 actaatggca atacgtgggg caggagtgtc ctgctccacg gtgtctgatt tttatcgcat
     6421 tacagaaggc acaggccaga aggtagggca atgttaaaat ttattctacg tcgttgtctg
     6481 gaagcgattc cgacgctatt tattcttatt actatttcgt tctttatgat gcgcctcgcg
     6541 ccgggaagcc cttttaccgg cgaacgtact ttaccgccag aagtgatggc caatatcgaa
     6601 gcgaaatatc atcttaatga tccgatcatg acacagtatt tcagctacct gaaacaactg
     6661 gcgcatggcg atttcggtcc atcgtttaaa tataaagatt attcggtcaa tgacctggtg
     6721 gcatccagtt ttcccgtttc tgccaaactg ggagccgccg catttttcct tgcggtaata
     6781 ctgggtgtta gtgctggcgt tattgccgca ttaaaacaaa acaccaaatg ggactatacc
     6841 gtgatggggc tggcaatgac cggggttgtt atccccagtt ttgtggttgc gccattatta
     6901 gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg gtggctggaa tggtggggcg
     6961 cttaaattca tgatattgcc gatggtggcg ttgtcactcg cctatatcgc cagtattgcg
     7021 cgtattaccc gtggctctat gattgaagta ttacactcca actttattcg tactgcccgg
     7081 gcgaaagggt tacctatgcg gcggatcatt ttacgccacg cattaaaacc tgctctgtta
     7141 cccgtgctct cctatatggg ccccgcattt gtcggcatta ttaccggttc tatggtcatc
     7201 gaaaccattt atggtttgcc ggggattggg caactgttcg ttaatggcgc attgaaccgt
     7261 gactattcct tagtgttaag cctgaccatc ctggttggtg cgttaaccat tttgtttaat
     7321 gccattgtcg atgtgctata tgcggttatc gacccgaaaa tccgttactg atactggagc
     7381 tcgcgatgat gttaagtaag aaaaacagcg agacgctgga aaatttcagt gaaaagctgg
     7441 aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg ttttatgcat aaccgtgcgg
     7501 cggttgccag tctgatagtg ctggtgctga tcgcgttatt tgtaatcctg gcaccgatgc
     7561 tttcccagtt tgcctatgac gatactgact gggcgatgat gtccagcgcc ccggatatgg
     7621 agtccggtca ctactttggt actgactcat ccggtcgcga cctgcttgtg cgcgttgcga
     7681 ttggcgggcg tatctcactc atggtcggtg ttgctgcggc actggtggca gtggtcgtgg
     7741 ggacacttta tggttcgctt tccggttatc tgggcggtaa agtggattcg gtaatgatgc
     7801 gtctgctgga aatcctcaac tccttcccat tcatgttctt cgtcattttg ctggtgacct
     7861 ttttcggcca aaacatcctg ctgattttcg tggcgattgg catggtttcc tggctggata
     7921 tggctcgtat tgtgcgtggg caaaccctga gtctgaagcg caaagagttt attgaggcgg
     7981 cacaagttgg cggtgtatcg acgccgggca ttgttattcg ccacattgtg ccgaacgtac
     8041 tcggtgtggt ggtggtctac gcatcgctac tggtgcccag catgatcctc tttgaatctt
     8101 tccttagctt cctggggttg ggtacgcaag agccgttaag cagctggggg gcattgctga
     8161 gtgatggcgc gaactcgatg gaagtctctc catggttact gttgttccca gcgggattcc
     8221 ttgtggtgac actgttttgt ttcaacttta tcggcgatgg cttgcgtgat gccctcgacc
     8281 cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa actgcaactg tgccgctcgc
     8341 acaacaacag gctgacgcac tgctgaacgt gaaagatttg cgtgtcacct ttagtacccc
     8401 ggacggcgac gtcacggcgg tcaatgattt aaatttttcc ctacgtgccg gagaaacgct
     8461 gggcattgta ggtgagtctg gttcgggtaa atcgcaaact gcatttgcgt tgatgggcct
     8521 gttggctgcc aacggacgca ttggcggatc ggcaaccttc aatgggcgtg aaatcctcaa
     8581 tttgccagag cgtgaactca ataaactgcg cgctgaacaa atctcaatga tttttcagga
     8641 cccaatgact tcgttgaatc cctatatgcg cgtcggtgag cagttgatgg aagtgctgat
     8701 gctgcataag aacatgagca aagctgaggc gtttgaagag tcggtgcgga tgctcgatgc
     8761 ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac ccgcacgaat tttctggcgg
     8821 catgcgtcag cgagtcatga ttgcgatggc attgctatgt cgacctaagt tgctgattgc
     8881 ggatgaaccc actacagcgc tggacgtcac cgtacaggcg cagatcatga cgctattgaa
     8941 tgaactgaag cgggaattta atactgccat cattatgatc acccacgatc ttggcgtggt
     9001 ggcggggatc tgtgacaaag tgctggtaat gtacgccggg cgcacgatgg aatatggcaa
     9061 tgctcgcgat gtcttttatc aacccgttca tccttattct atcggtttgc tcaacgcggt
     9121 gccgcgtctt gatgcggaag gtgaaacaat gttgaccatc ccgggtaatc cgccaaactt
     9181 gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt ccacatgcga tggaaatttg
     9241 tagtagcgca ccgccgctgg aagagtttac gcctggccgt ctgcgtgctt gctttaaacc
     9301 ggtggaggaa ctgttatgaa tgctgtaact gaaggaagaa aagtcctcct tgaaatcgcc
     9361 gatcttaaag tgcactttga aatcaaagat ggcaaacagt ggttctggca accgccgaaa
     9421 acgctcaaag ccgtcgatgg tgtcactctt cgcctgtatg aaggggaaac attaggtgtg
     9481 gtaggggaat cgggatgcgg taagtccacc tttgctcgcg ccatcatcgg tttggtcaag
     9541 gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc tgggcatgaa gcccgatgaa
     9601 tggcgtgccg ttcgcagtga tattcagatg attttccagg atccgttggc atcgctaaac
     9661 ccgcgtatga ccatcggcga gatcatcgct gaaccactgc gtacttatca tccgaaaatg
     9721 tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc tgaaagtcgg gttattgcct
     9781 aacctgatta accgctatcc gcatgagttc tccggtgggc agtgccagcg tatcgggatt
     9841 gctcgtgctc ttattcttga accgaagctg attatctgcg atgagccggt ttcggcgctg
     9901 gacgtgtcaa ttcaggcgca ggtggttaac ctgctccagc agctgcaacg tgagatggga
     9961 ttgtcattaa tttttattgc tcatgacttg gccgtggtaa aacacatttc cgatcgtgtg
    10021 ttggtgatgt atctcggcca tgcagtagaa ctggggacct atgatgaggt ctaccacaat
    10081 ccactacatc cttacaccag ggcattgatg tcggcagtcc ccatacctga tccggatctg
    10141 gagaagaaca aaaccatcca gttactggaa ggggaattac cgtcgccgat caacccgcct
    10201 tccggttgtg ttttccgtac ccgttgcccg attgccggtc cggagtgcgc caaaacacgt
    10261 cctgttctgg aggggagttt cagacacgcc gtttcttgcc tgaaagtcga tccgctttaa
    10321 aaacaataag ggctgacagt tgtcagccct ttttcacgct aaaagcgatt atttattccc
    10381 gccagatgat atggcaaagt ttgtgatctt tttcgcggca taacagaatg cgggcgaaaa
    10441 catcgttgat ttcaccatct tcactgtccg ccagaccaat caccacttcg gcaaaaaagt
    10501 ccgggttcaa atcgaaatca acatgttcct gccagtcttc cgccggatcg aataactccg
    10561 cgccgccgcg ctcttcaaac tgaagattga acagcagaac atcagctgga tcgagattgt
    10621 cggcagccag ttcgagaaaa atatcgtagg cctgctcaag cgtttcatct tcagtcaggc
    10681 gattgtttag atccatatcc atagtcacta cctgtttaac ctctgttggc gacgttttac
    10741 agcaacgggc tgaagaagta aaacagtcgc tcggcgacac gttgccataa tggacgtttt
    10801 agccataaac gggcatcgag cagacgtgaa cgcgaaatat aatcgtcctg aacggcggca
    10861 aggtctgcac caaaaccttt atcgtcgatt gccaaggtaa tctcgaaatt cagccacaga
    10921 ctacgcatat caaggttaac ggtgccaacc agacttagtt cgccatcgac cagcacgctc
    10981 ttggtatgca gtaacccgcc ttcaaactga taaattttaa ccccagcagc aagcagttcc
    11041 gtaaagaatg cgcgactggc ccagccgacc agcatcgagt cattttttcg cggaaggata
    11101 atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg catgaagtaa atcatcgctt
    11161 ggcacaaagt agggcgtggt catgatcaaa tattcatgcg ccgaataagc cgcagtcaat
    11221 aatgcctggt gaatgagatc ttccggaaag ccggggccag aagcaattgt gtgaatggtg
    11281 tgaccgctgg cctgttcaaa cggcataata ttgacatctg gtggtggcgg cagaatacgt
    11341 tttccggttt caatctccca gtcgcaggaa taaataatcc ccatcgcggt ggcgatgggg
    11401 ccttccatac gcgccatcag atcaatccat tgccctacgc ccgcatcttg tttgaagtag
    11461 cgaggatcga ccatattcat gctgccggtg tacgcgatgt aattatcgat catgatcatc
    11521 ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac gcatcagatt gacctttaag
    11581 gcttcgacca cttcaatacc ggcattacgc attagctcgg gccacgggct gcggaaaaaa
    11641 gccacactcc cggcggagtc gagcatcaat cggcaatgaa taccacgccg tgcagccgcc
    11701 attaatgatt cagccacctg gtccgccatg ccgccgggct gccagatata aaacaccatc
    11761 tcaatattat ggcgcgcgag ctggatgtcg cggattaacg cctgcatcac atcatctgac
    11821 tcggtcatca gctgtagctg attccctttg accccagcga tcccctgacg acgctcgcaa
    11881 agcttgaata atggcgcagc gacactgcta ttttcttcgg cgaagatatg cttacaggct
    11941 ttaaggtcgt taagccattt tgcggtggaa ggccacatcg ctctggcgcg ctcagcgcgg
    12001 cgtttgccta aatggagctc gccaacggca agataggcaa taattccgac taacggcaga
    12061 atgtaaataa tcaacagcca ggccatcgcg gagggaactg cgcgtcgttt cattagaatg
    12121 cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa tggccaacca actcaccaac
    12181 gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc gcattgttat gagtttacgc
    12241 atatggattc atctggcaaa taaaaacgcg ggaaaagcac tggtcagcgt cgggggcggg
    12301 ggtataatga ccattctgtt attgcataga gtagttaaca tgaagcggag tagaacggaa
    12361 gtggggcgct ggcgcatgca gcgtcaggct agccgacgta aatcgcgttg gcttgagggg
    12421 caatcgcgcc gaaatatgcg tatccacagc atcaggaagt gcattctaaa caaacagcgt
    12481 aactcgttat tgtttgcgat ctacaatatc taaatgtact gggcaccatt gcggtgccca
    12541 ataagtttac tcttccgtct ttaaactcta tttttgcgcc gattctttac tgtcagcttt
    12601 cgtttcttgt tcatcgttat cgctgaaaat accatgacct gagttcaaca acattgaaac
    12661 gagcatatca ttattaatct cttcaccatt taaaactcgc gccagaattt cgctgccaaa
    12721 cagttgcggt gagagaatga tatccggatg taccatctta atcttattca ggtttttgct
    12781 atcactgacg gcgagaactg ttttgacatc actgctcata tcttttgccg agagtacaac
    12841 aaacgcgtta tctgcatcgt tatcactcag cgccagaatg gcccggcaac gatcgattcc
    12901 cgctttcttt aatactgaac tgtcattact gtcaccgggg ataacatcag cgttatcgcc
    12961 taagcgttgc tcaagttgct tgatatcatc ttcaggcaag ttgctgataa ccgttacgtt
    13021 ttgtccgcgt tgattcagtt gcaggatcgt attgatggcg agaatcgaat gtccgcaaac
    13081 aataaaatga tctttacgat gcattgtatg attgtttcct tttaaaagtt tgttgaatcc
    13141 cccgcggata agcggaccaa aaattgaggt catagatgtg gcaaaaacgg taatgccgga
    13201 aataatgacc gaaatagtga acaatcgtgc tgattcagaa acagggacaa tatctccata
    13261 gccgacggtt gacatggttt ctatcgaaaa atagaacgcg gtcatcaaac tttctattcg
    13321 cggattaaaa ccttcgctta aataaagcgc accgtaggtt gagtaaaaca gtaatgtcgt
    13381 gaaactaata aatgcaaaaa ttgtcccggc tgcggcgcta ctgtgggaga agtctttgcg
    13441 cagtatcagc aaaaacacca gcgtaaaaat gcaaaatcca atactaaatt tcagccaggg
    13501 ataaaaatgc agggtgtaaa ttagcgctat caacaacaaa atgatactga ttgcccatgc
    13561 gagctttgcc cggaacagta agccaatgga gttcaggaca agaaagacgc cgagcataaa
    13621 tagtggggcg tttgccagca aggagagatc caactctgaa aatgctttga tatggaaaat
    13681 atcaagcaaa ttgaccgaca taccatagat tgttttaaaa ataagcaatc catttaaaaa
    13741 gacggccagc gcgagaatgt cgtgccgtag ggtcacccat aaatttgttg ctgtttgttt
    13801 gaatgtagcc cagtgactca ctgatacctc tgtaaccaca attttgttta attatcttag
    13861 cgtgcacatt ttctgaacat acatgcagcg cgtgaatgtg ttaaacaata gtagatgact
    13921 ttatccgcag taatcaaagg ttttgagtga gcaactctgc gtcaggaaag ggcttttttc
    13981 ctgaacatgc tcacatcata taaagaaata cagataaagt tattatctgc ttgtggtagt
    14041 gaatgcactg accggctata aggaaaggcc aaacaagaac acggttgcaa aaaccgtgcc
    14101 cttaaatatt ggatctct
//
LOCUS       DAEEDS010000095        13963 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEEDS010000095 DAEEDS010000000
VERSION     DAEEDS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.095"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KLH32_003270"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KLH32_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0139994.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KLH32_003271"
     CDS             2219..2767
                     /locus_tag="KLH32_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBE0139995.1"
                     /translation="MGKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KLH32_003272"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KLH32_003272"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBE0139996.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KLH32_003273"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KLH32_003273"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBE0139997.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KLH32_003274"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KLH32_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBE0139998.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLH32_003275"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLH32_003275"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBE0139999.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KLH32_003276"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KLH32_003276"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBE0140000.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="KLH32_003277"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="KLH32_003277"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBE0140001.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KLH32_003278"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KLH32_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBE0140002.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KLH32_003279"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KLH32_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBE0140003.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3562 c         3526 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 gggcaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DAEEDS010000096        13950 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEEDS010000096 DAEEDS010000000
VERSION     DAEEDS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.096"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..231)
                     /locus_tag="KLH32_003280"
     CDS             complement(<1..231)
                     /locus_tag="KLH32_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140004.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTLAELESEILQLRKALNEA"
     gene            complement(295..486)
                     /locus_tag="KLH32_003281"
                     /pseudo
     CDS             complement(295..486)
                     /locus_tag="KLH32_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(627..2660)
                     /gene="metG"
                     /locus_tag="KLH32_003282"
     CDS             complement(627..2660)
                     /gene="metG"
                     /locus_tag="KLH32_003282"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBE0140005.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2792..3901
                     /gene="apbC"
                     /locus_tag="KLH32_003283"
     CDS             2792..3901
                     /gene="apbC"
                     /locus_tag="KLH32_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBE0140006.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4163..4444
                     /locus_tag="KLH32_003284"
     CDS             4163..4444
                     /locus_tag="KLH32_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBE0140007.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4737..5261
                     /locus_tag="KLH32_003285"
     CDS             4737..5261
                     /locus_tag="KLH32_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0140008.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5342..6016
                     /locus_tag="KLH32_003286"
     CDS             5342..6016
                     /locus_tag="KLH32_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0140009.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            6032..8512
                     /locus_tag="KLH32_003287"
                     /pseudo
     CDS             6032..8512
                     /locus_tag="KLH32_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8528..9561
                     /locus_tag="KLH32_003288"
                     /pseudo
     CDS             8528..9561
                     /locus_tag="KLH32_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9643..9981)
                     /gene="rcnB"
                     /locus_tag="KLH32_003289"
     CDS             complement(9643..9981)
                     /gene="rcnB"
                     /locus_tag="KLH32_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBE0140010.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10200..11048)
                     /gene="rcnA"
                     /locus_tag="KLH32_003290"
                     /pseudo
     CDS             complement(10200..11048)
                     /gene="rcnA"
                     /locus_tag="KLH32_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            11170..11442
                     /gene="rcnR"
                     /locus_tag="KLH32_003291"
     CDS             11170..11442
                     /gene="rcnR"
                     /locus_tag="KLH32_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBE0140011.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11534..11630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11665..12453
                     /gene="thiM"
                     /locus_tag="KLH32_003292"
     CDS             11665..12453
                     /gene="thiM"
                     /locus_tag="KLH32_003292"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBE0140012.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12450..13250
                     /gene="thiD"
                     /locus_tag="KLH32_003293"
     CDS             12450..13250
                     /gene="thiD"
                     /locus_tag="KLH32_003293"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBE0140013.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13315..>13950
                     /locus_tag="KLH32_003294"
     CDS             13315..>13950
                     /locus_tag="KLH32_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBE0140014.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEY"
BASE COUNT         3723 a         3133 c         3421 g         3673 t
ORIGIN      
        1 agcttcattt aacgccttac gcagttgcag aatttcagat tccagttcag ccagcgtgcg
       61 ggaaccagga gtaccgagcc cttttctggc ggcggtaacc cattgtccta aagtgccttc
      121 aggaagagat aatcgggagg cgccttcact gatcgaaagt tgattttcaa gaaccgttct
      181 gacagcttcg gctttgaact ctttagagta acgttgggtt tttctgctca ttattagctc
      241 cttctgatgc cattctattt caggaaggag tgtccgttaa actcaggcta cctcataccc
      301 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
      361 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      421 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      481 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      541 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      601 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      661 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      721 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      781 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      841 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      901 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      961 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
     1021 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
     1081 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
     1141 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
     1201 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
     1261 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1321 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1381 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1441 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1501 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1561 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1621 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1681 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1741 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1801 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1861 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1921 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1981 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     2041 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     2101 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     2161 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     2221 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     2281 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2341 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2401 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2461 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2521 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2581 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2641 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2701 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2761 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2821 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2881 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2941 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     3001 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     3061 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     3121 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     3181 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     3241 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3301 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3361 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3421 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3481 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3541 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3601 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3661 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3721 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3781 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3841 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3901 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3961 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     4021 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     4081 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     4141 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     4201 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     4261 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4321 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4381 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4441 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat
     4501 ttcgtctttt ttattgatat caacaatacc atttacataa atctacatta attaatagat
     4561 gtgataaata tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt
     4621 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
     4681 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
     4741 aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg
     4801 ctgcagacgt tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta
     4861 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
     4921 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
     4981 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
     5041 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
     5101 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
     5161 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
     5221 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
     5281 ctccaggtaa taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa
     5341 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
     5401 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
     5461 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
     5521 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
     5581 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
     5641 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
     5701 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     5761 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
     5821 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     5881 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     5941 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     6001 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     6061 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     6121 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     6181 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     6241 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     6301 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     6361 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     6421 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     6481 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     6541 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     6601 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     6661 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     6721 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     6781 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     6841 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     6901 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     6961 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     7021 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga
     7081 aggggcgagc aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt
     7141 attgacgctg tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac
     7201 gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca
     7261 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     7321 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac
     7381 atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat
     7441 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     7501 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     7561 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     7621 gcggataagc tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa
     7681 acgttttaat atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg
     7741 tcggcaaata tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa
     7801 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     7861 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     7921 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt
     7981 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     8041 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     8101 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     8161 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     8221 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     8281 ctggtttata aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa
     8341 tgatatccat ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac
     8401 caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     8461 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     8521 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     8581 ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt
     8641 tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt
     8701 gagtacaaat actatcaccc ctatgagccc atcggatatt attgttggac tttataacga
     8761 taccattaaa ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa
     8821 taatcagagc agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc
     8881 aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt
     8941 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     9001 aggtggcaag tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc
     9061 aggtgtctat tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca
     9121 ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa
     9181 caaagcaaca ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg
     9241 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     9301 caacagcaac acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt
     9361 aatggttcgc cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta
     9421 aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc
     9481 agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta
     9541 atcaacgtga aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata
     9601 acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat
     9661 ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc
     9721 acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg
     9781 cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg
     9841 cttaaagtca gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga
     9901 acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa
     9961 catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata
    10021 tctttcctgt catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg
    10081 taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga
    10141 aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt
    10201 tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac
    10261 agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg
    10321 acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca
    10381 atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc
    10441 aacagcacgg taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa
    10501 atttgccagt tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct
    10561 cgtgcatggg catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg
    10621 tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca
    10681 tgcccgtgca tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag
    10741 aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga
    10801 gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta
    10861 tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
    10921 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
    10981 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
    11041 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
    11101 cctgatacta cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca
    11161 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
    11221 attcagggcc aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca
    11281 gttttacaac agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt
    11341 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
    11401 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
    11461 aaactgccgt ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga
    11521 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
    11581 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
    11641 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg
    11701 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
    11761 gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc
    11821 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
    11881 acactgacgc agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
    11941 caaacacgct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
    12001 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
    12061 gcattagctg gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac
    12121 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
    12181 gaggtggatt atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta
    12241 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
    12301 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
    12361 gaacgcgcag tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg
    12421 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
    12481 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
    12541 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
    12601 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
    12661 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
    12721 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
    12781 aaagcggcga cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc
    12841 cacaggtttc attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac
    12901 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
    12961 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
    13021 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca
    13081 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
    13141 acaccgtaca ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg
    13201 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
    13261 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
    13321 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
    13381 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
    13441 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct
    13501 gccagttaca ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag
    13561 cgggtggcaa aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg
    13621 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat
    13681 ggcctgctgc gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg
    13741 cgattatttc tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta
    13801 gacgaacggg gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa
    13861 atggtcgaaa aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac
    13921 accaatctgg cgggctattt caatgaatat
//
LOCUS       DAEEDS010000097        13800 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEEDS010000097 DAEEDS010000000
VERSION     DAEEDS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.097"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="KLH32_003295"
     CDS             complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="KLH32_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBE0140015.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGT"
     gene            complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="KLH32_003296"
     CDS             complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="KLH32_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBE0140016.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="KLH32_003297"
     CDS             complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="KLH32_003297"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBE0140017.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="KLH32_003298"
     CDS             complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="KLH32_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBE0140018.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="KLH32_003299"
     CDS             complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="KLH32_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBE0140019.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="KLH32_003300"
     CDS             complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="KLH32_003300"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBE0140020.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6044..7183)
                     /gene="wza"
                     /locus_tag="KLH32_003301"
     CDS             complement(6044..7183)
                     /gene="wza"
                     /locus_tag="KLH32_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBE0140021.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7492..7641
                     /locus_tag="KLH32_003302"
     CDS             7492..7641
                     /locus_tag="KLH32_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140022.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7842..9425
                     /locus_tag="KLH32_003303"
     CDS             7842..9425
                     /locus_tag="KLH32_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE0140023.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="KLH32_003304"
     CDS             complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="KLH32_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBE0140024.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="KLH32_003305"
     CDS             complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="KLH32_003305"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBE0140025.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12425..13066)
                     /gene="udk"
                     /locus_tag="KLH32_003306"
     CDS             complement(12425..13066)
                     /gene="udk"
                     /locus_tag="KLH32_003306"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBE0140026.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13384..>13800
                     /locus_tag="KLH32_003307"
     CDS             13384..>13800
                     /locus_tag="KLH32_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HBE0140027.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3193 a         3640 c         3495 g         3472 t
ORIGIN      
        1 gtacctaaat cgcccagacg ggtggaaata taaggaaacg cgattaccac accaacccgg
       61 aatacagcca gagaaatcaa tgctaacggt aattttttct taatcgcctc tttattcaga
      121 tattgaaatg cccactccag caaataaaac aggataaagg tcataacccc tgaaaacgat
      181 ccggataata ttatccctgc gagaatcagg gcatctgttt taggcgtttt gataccaaac
      241 tgtttgatgc tgagccaaat tgagattaat gccagagcga aaaatgccgg ttcgaaataa
      301 agtgctgtcg tgcgcttgcc gccgaattta atgaaattca gcacatagct gttgctgtaa
      361 atcagatatt tcgaaatcga ctccataata ctgctgccac cggtgaggat aatttgcgcc
      421 atctccaccg ccgccagcgc caccaccagt ccgaccacca gataaaagaa acgtaatatc
      481 ttacgatggt tgtgtggcga aattgtttta aaacgaatac tccacaccat gccaataatg
      541 atcacaatat agacaaacag catagttgaa gtgacgtatt taccggcatc caacgactga
      601 ccaaacaggt agttaaacgc cgtgagtccc gcgccaatcc ctaaggcaat catcaatttt
      661 ttaatgctga tgcgttctaa aaacagcagt aacaagacag gtaaaaaagt gacgatggtg
      721 atggggaaac tttcgccaag ctgggcgatt ttgacgttaa ccagcaggta gatcagtggc
      781 agcagcaggt agctacagat tctgatagaa gttgacatac tcctccagca tctgttgtcc
      841 actgtaggcg gcgcgactgc gttggctgaa ctcagccagc gtggtaccaa atatcgcctg
      901 cgcgatttcc ggtttgctta actgtaccag ttgcagcacc tcttcttcac tgacggtttt
      961 accaccggat ttttgcagca cttcccgcgc cgcatcgcta tgggtggcaa tcaccggcac
     1021 gccaatcgat agcgcctcac acaaaatcag cgggtagtta tcgacacgag aactgaacac
     1081 cagcgcatcc atctgattga gcgcgctcat caacttgcgc ttgtcagttt caaagccgtg
     1141 attaaccacg ttgccagcgg tgaacggcga gaacttacca aaggtatgca gttcgatttt
     1201 gtcgcccagc gccatcatct cacgcaccag ttgctggtta gttttgccgt cgtaacgcag
     1261 gtcatgcgcc accactgcga ttttcggctt gccctgagtt tcgcgcaccg gaggcagatc
     1321 tgccagaatc gcttcggttg ccatatcaat gccattattg ataatccggc aacgccctgg
     1381 cccgtacaga ctattgaaag cgtcagccac atgctggctg ggggaaataa actgacagcc
     1441 cagcgccagt atctcgcgga ataactggcg tttgcccgcc accagctggt gcgcgcgatc
     1501 aatcttcacc ggcggatagt tatttaaggt cgggcatttc tggcagcccg ttttccagcc
     1561 ttcgcaaccg tcggtaaagg cgcagcgtcc ggttacactc cagtggtcgt gcagcgtcca
     1621 gaccagggtg acatccggtt tatggttttt cactttttcg caaaagcgca ccacgctgtt
     1681 aagattcaac cagtagctgt gcagcacatg aaaatgcagg accaccggac ccggtgtgcg
     1741 agtaatggtg cgatataact cattgaaatt gccgaacaga tcgcgattaa acagacgaaa
     1801 caacgcaatg ttcgccatcg cggtcatccg cggcgtatgt ttgatgacct gcggatagtt
     1861 ctgatggctg acgctctctt tgccgccttt gccgtagccg tagacaaaat gtgacgccag
     1921 cccctgttgc agcgcacgct ggtggagatc taacgccaca cctgctgccc cgccttccgc
     1981 cagtcgcaca ttaaattgca gaatattcat ttaatcacct tcactcgcgc tttttctccc
     2041 accaccagcg cgttgtccgg gacagagtcg agcaccacgc tgcccgcacc cacggtgacg
     2101 ttgttaccaa gcgtgatatc accaagaata atgacgttgg caccgagttc gacgccgttg
     2161 ccaatgtgtg gacatgccat gttatcggca ccccgattgc cgatagtgac gccgtggcga
     2221 atggtgaaat catcccccgc caccacgttt ttattgatca ctacggcgta accgtgatgg
     2281 atagtaaagc ggcggccaat ggtcgcggcg gcctggattt cataaccgaa aaagcattcg
     2341 gtgataatgc gatacagcac cagcagcggg gccgcccaca gattgttgag gacgttcttt
     2401 ttgcgccaca ccgaacaaaa atgagcgacg cgataggcaa gtaccatgca gcacgggcgt
     2461 aaactccagc tgttggcgcg cagatcttcc agcatcttta gcgcccccga ataccatcag
     2521 ccagacgttt gccgttacgc accgacagca gcgtcaacaa ggtgcgccag gtcatacgtt
     2581 tattgcggat ctggtagagg gtaaacagct gatatttttt actggcgcgg tcgaatttgt
     2641 ctttgtgctt gcggtaaaag tggaagtatc cggagaattt tttcggtgac gaggtgatct
     2701 gcatttcgcc gtgattgata tgcaggatct gcgtcgcctc ttccactttc cacggttcgc
     2761 cgtactccac caccatccgc aggaatatgt cgtaatcctg tgctgctttc agttcggtat
     2821 cgaacaaaca ctctttgaaa cgccatgccc aggtaaagac ctgattgcca atgatattac
     2881 gtttatagaa caggcggcgt gagtacggcg atttgggata cagtggtaag ctcgccggtt
     2941 gggaataaac ttctccctgg caaacgtagt cgttagcgta caaaaaggcg tgtgtgacca
     3001 gttgctgttt atgggcgagg aagacgctca ggcggttggg cgtccattca tcatcatcat
     3061 cgatcccggt gatgtattcc ccttgcgcca gcataatcgc ctggttacgc accgcgcacg
     3121 ccccgctgtt aatgtcgttg tggatgtaag tgatacgcgg atcgttgagg gcagtgacgt
     3181 actgttgcag ttgttcccag gaggtggagc aatcatccac gatgatcatc tcccagttgc
     3241 tgtagtcctg gcgcaggacc gattttatcg cccgaatcgc cagttgttgg cggttccagg
     3301 ttggcatata gattgagatc agcggattgt ctttcatagg ttgttctccc ggcttatgag
     3361 ctggtttgct tgccggatgc ggcgtgagcg ccttatccgg cctacggggc ggtgcgaatg
     3421 caggcgtcgt tatttcgcat ccgacttata ttcgtattcg taatagccat aatcctgata
     3481 cgcgctggcg cggcggaaga tggagttcag aatcacccct ttcaccggaa taccgttttg
     3541 ctcaaagcgg ctcagactgg tttccacttc tttcaatgtg ttgaccgcat aacgcgccac
     3601 cattaacgtg gttccgacat gacgaccaac aattgccgca tcggtcactg ccagaatcgg
     3661 cggcgtatca atcaacacca ggtcatagtt tttactcgcc cagttcacca gttcggcaaa
     3721 gcgttcgctc attaacagtt cagaaggatt tggcggcacc tgaccgcgcg ggatcaggtc
     3781 aaatttggca atagaggtcg gtttagcagc ggtagtaata tcgccctgac caatcagaat
     3841 ctccgacagc ccattgacat tattggtgcc caacagctcg tgggtgtagc ctttgcgcat
     3901 atcgcagtcg atcaacaaca cgcgtttatt ggtctggctg atcaccgccg ccaggttggc
     3961 gcagacaaag gttttaccga ttgacgggct aaccccggtc atcatcaaca cattgttctg
     4021 cgcctgcatc atcgcgaagt gcagactggt acgcagacta cggatggctt caatcgccag
     4081 atcggttgga ttccccactg ccagtagctg gctctgttta tagcgtttaa cccctttgat
     4141 ggttttgacg ctatcgcgcg ctttctgcca ctccgacagc gggatgctgg catagacgct
     4201 gattccgtgt tcttccagca cttgcgggct ttcgatgccg cgattaaaca acgagcgcag
     4261 cagcacaccc acgatagaga gcatcaggcc aaggataatc gcgccgagga taatcagccc
     4321 tttcttcggt tttagcacgc caggctgagt gattgccggg tcaataatgc gcacatcgcc
     4381 gacggtgctg gcttcggtga ttttcagctc ctgctcttta ttcagcagtt gcatatagac
     4441 ctgctgacca gactcgacat cgcgggtcag acggacaatt tcctgctggt ttttcggcat
     4501 cgccgttacg cgaccgttaa gtttggcttt ctcttcttcc agcgcctgac gtttctccag
     4561 cagcgtgcgg tacgccgggt gaactttggt gtacagcttg gagatttccg cctctttaaa
     4621 ggtcagttcg ttcaactgcg cgtcgatgtt caccatcgaa tcgagcaccg ctttcgcttc
     4681 aagcggcaga tcaacagaat ctttatcctg acggaaggca ttcagtttgt tttcggcaac
     4741 atcgaggcgg ctacgtactt ccggtaactg ttgcgcgagg aaagcgaggc ttttcgacgc
     4801 ttccgccgat ttgcgctcaa tattttgttc ctgatagtta cgagcgatgc tgttaagaat
     4861 gtcgcggatc tgttcgcgat cttcaccggt ataagtcagg ctcagtacgc ctgcgtcttt
     4921 gccgttctcc gttaccgtca ggctgttttg cagttggttg atcatcccca acgtggagta
     4981 tttggtgacg gtaaactcac tgcccgggcg ggcgtgaatg gcttcaacca tcagcgtgac
     5041 gccttctttt ttcagcatct ggcccgcttg cccacgggcg ctaaagccgc catcactgct
     5101 cagggtgtag tttttgtcat ccagcacatt aagcgtaaac acctgatccg ccatctcttt
     5161 cgggcggtta aaggtagtca ctttcaccgt ctcgttctga cgccccatca ggcgatccca
     5221 gcccgccccg aaaatcggga acgtgttttt gctcaccgca atatcgaggt cgagatcgtc
     5281 caccgtttta ccaagcacca gacgcgaacg aatcaactgg atctcggcgt ccgatgcagg
     5341 cggtttgttg gctaatgccg agccgatatc ctgcaccagc gaattgccgc tgttttgctc
     5401 gatttgtacc agtgcgtcgg cactataaat cggcgtggcg aagaaagtgt aaaccacggc
     5461 acagagggca aatacgacgg tgatgccaat cacccaccag cgcgcttcaa tgacggtgcc
     5521 gaccaggcga ccaatatcga tttcatcact gcccgttacc ggagcggcat gttgttttac
     5581 tttttctgtc attcttatac ctgctctgcg ttcaatgcct gcgcccactg gcgggcagac
     5641 cgttcaagta atgtgtacac cgctgcaaac gtttcccggc ttttgcgata cggatcgggg
     5701 atttcacatt cgttatccca gtgaccaaac agcatcactt tgccgcgcat ctccggtgcc
     5761 atctcgcata agcgttcgat atggcgctta tccatggtca aaatcaggtc atagttgcga
     5821 cacaggcggc ggctgatttg tcgtgcacag tggccttcca gagacagttg atgttctgcg
     5881 gcgacgctga tagcggtagg atcagcgccc ttaccgacca gcgcgccgag tccagcggac
     5941 tccactttca gttccgggtg ataacgttgc agtaagcgtt ccgccatcgg ggaacggcaa
     6001 atattgccga cacagacaac taagatgttg ttaaacatga cgattaccag ttatgaatgt
     6061 cgctggctgt atccgtcatg taacggacac cgctaatagt tggcagcaac tgattgatca
     6121 gacggttcca gcgggaaacc ggggcggtgg tgacatacac cacgtcataa ggttgcaggc
     6181 ggaattctgt cgccatcacc agcgacgtcg catcggacat atccagctgg tagatattgg
     6241 caatcttgcc gttacgcccg ccctcgcctt tcagcggacg aatgacaaag atgccgctgg
     6301 cgttggaggt ggtcatgtcg ataccttcgg cattggccag agcttcagtc agggtcatgc
     6361 cgctaaagtc cattttcagg gtgctctgtt tcttcacttc acccatcaca aatactttca
     6421 gatcatcatt acgcggcacg tagagaatat cgccgggata aagcagtcgg ttctgattga
     6481 ggtcgccgtt ttgcatcagc gcctgtaaag aaatgcgttc ttcacgacca ttgtgtgtta
     6541 gcaccacgtt gcgccagtca gcggtgtcgg tcaggccacc tgcggcgttg atggcgtcga
     6601 gaatggtcag tggtacgttg gtgatcgcct gttgaccgga tttattcacc tgacctgaga
     6661 tataggcctt ttgtgagcgg aaggcggcga tattaacgtc cacctgcggg tcagcgatgt
     6721 acgtcgctaa gcgcccggta atatcactgc ggatttcagc gagcgttttc ccgactacgt
     6781 gaaccttgcc gatatacggg taaaacatag tgccgtcagg ctgtacccag ttgccggtgt
     6841 cgctggagct gcggtactga ccagctggcg tggtgagttc cgggtgatcc cagacggtga
     6901 cattaagaac gtcccccggc ccgacgcgat actggtaatt cgcgatctcg ctttccagcg
     6961 tcatattggg gcgcgctaca ttcgggcgtg ggcgtaattg gtcaatcagg cgcggggtca
     7021 gcggataaac attcaccatt ttgtcgagat cgaaatcagc gtcctgctgt ttgatgacgt
     7081 ctttgcccat cgtcgacata ttgctgcccg gaagtactgt gcaaccgctt atcaaggtta
     7141 ctgacaccaa taatggcatc aatttcattt tggatttcat cattgtttat ttatcacttt
     7201 ggcagagtaa ttatcctgtg cactattaat agcaatgtcg ccatgcacat ttaccttgca
     7261 gttaattgaa taaaaattta actggcatca gtcctaaaaa aattgatttc atccgcaggc
     7321 tattgacaga ataattcaga ctggtctttc aggcatccag acacgctacc gcccctggct
     7381 ttttagctac caatacactg attgagctta atttttcaaa ccctctctgc atacaatcgt
     7441 tgatgagaaa gggttattgc ggaaattaac ttccgaatat aaggtgacat tatggtaatt
     7501 gaatattggc tcgccaataa tgcaggagga agtgttacag ctaacggaat agcaggcaag
     7561 gtaacaattc ggcaattggc tatttttaag aattatatta agtatcattc aatatggttt
     7621 ttaggagttt ctttaggttg acaatattta atattgcgtc tccacatgcg atatttctta
     7681 aataatgttt tattattacc gcttttaatt cagggataat gtgaggttat taccctcaaa
     7741 taatatgaga taaatagtgc tgcaacattg catttttgcc ccgatttatc catgatcgaa
     7801 ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac tatggaatgg attgccgatc
     7861 cgtctatctg ggccgggtta atcacgctga ttgtgatcga actggtcctc ggcattgata
     7921 acctggtctt tattgccatc ctcgccgaaa aactaccgcc gaagcagcgc gaccgcgcac
     7981 gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt actgctagcg tcaatctcct
     8041 ggctggtcac cctgactcaa ccgctgttca gcttccgctc gtttaccttt agcgcccgcg
     8101 acttaatcat gctgtttggt ggtttcttcc tgttgttcaa agccacgatg gagctgaacg
     8161 aacggctgga agggaaagac agcaataatc ccacacaacg caaaggtgcg aagttctggg
     8221 gcgtggtgac gcaaattgtg gtactggacg ccattttctc actcgactca gtgattaccg
     8281 ccgtcgggat ggtcgaccat ttactggtca tgatggccgc cgtggttatc gctatcagcc
     8341 tgatgttgat ggccagcaag ccgttaacgc aatttgttaa cagtcacccg accatcgtta
     8401 ttctctgctt aagcttcctg ttgatgattg gctttagcct ggtggcagaa ggtttcggct
     8461 tcgtcattcc gaaaggctac ctgtacgctg ccattggttt ctcggtgatg atcgaggcac
     8521 tcaatcagtt ggctatcttt aaccggcgac gttttctttc cgctaatcag acgctacgcc
     8581 agcggactac cgaagcggta atgcgcctgc ttagcgggca aaaagaagat gcggaactgg
     8641 acgccaaaac cgcgtccatg ctggtggatc atggaaacca gcagatcttt aatccgcagg
     8701 aacggcggat gattgagcgg gtacttaatc ttaaccagcg taccgtaagc agcattatga
     8761 cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagaagaa atccgccagt
     8821 tgctggagcg aaatcagcat acgcggctgg tggttaccga cggtgatgac gcagaagatt
     8881 tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc actgcgcggc gaaccgctca
     8941 acctgcgggt gttgattcgc cagccgctgg tgttcccgga aaccttgccg ttgttaccag
     9001 ccctggagca gttccgtaat gcccgcacgc actttgcttt tgtggtggat gagtttggct
     9061 cggtggaagg gattgtgaca ttaagtgacg tcactgaaac cattgccggt aacttaccga
     9121 acgaagtgga agagatcgac gcccgccatg atattcagaa gaatgcggac ggttcctgga
     9181 cggcgaatgg tcatatgccg ctggaggatc tggtgcaata tgtgccgctg ccgctggatg
     9241 aaaaacgtga atatcacacc attgccgggc tgttgatgga gtatttgcag cgtattccaa
     9301 aacctggcga agaagttcag gtgggggatt atttgcttaa aacgttgcag gtggaaagcc
     9361 atcgcgtgca gaaggtgcag attataccgc tgcgtaagga tggcgagatg gagtacgagg
     9421 tgtgatggta tcggccattt tacccaatgc tgcgtaacgc cttataactt cagcaatgaa
     9481 gcgttgtctg tggtaaacgg acatcacagc gggggtgtgg gtccggctgt aaatcgccga
     9541 atcgtttcct gtaggctggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg
     9601 gatgttacct cgcgcccgta cccgatagcc gaaaggaata agatgaggga accgcgccag
     9661 cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc caccctggca
     9721 cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg aacagccttc
     9781 gatcttacat attcagaata taatagtgcc gccgcgaacg cctgatgcga cgctttacgc
     9841 gtcttatcag gcctacagga caggtcacct ggtaacctac atcttctcca gcaacttctt
     9901 cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca ggcgacgttt
     9961 cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcaggctgt aattgagttg
    10021 ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat caatcagttt
    10081 gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc aggtttgatc
    10141 tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac cttgcatgtc
    10201 gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt caagccgcgt
    10261 cacgttatcg aagttttcag cggccttcac atcaccgcca ttacgctcta ccgcttgctg
    10321 aatcatctgc tggaagttca tcccttccat acgtgtgtcg gtcatctcaa catgcgcctg
    10381 tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga aatcaccagc
    10441 cagtgacatt tttccggtca acgaaatcgg atagttaaac gccttcagaa tggtaccaat
    10501 ctcaacgttc tccagccgtg gctggaagtt tatccgcgga tttattgatg tcgcgtccag
    10561 cgtgcccggc agtgaaacct ttccaccgtt aagcttgccc tgcagttgag taatttccag
    10621 caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc cgcgccagcg
    10681 cacgttactg gcctgcaaca aaatatcagc tgtaaagcct tgcagtccct gataggccgg
    10741 ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct gcccctgctg
    10801 cgcggcaccg ttttcaccat tcgcggtttc attaagcggg atgaggttgt caagattcag
    10861 ttgcgggaat tgcagtctca gctgccattc cggtttctct gtcagcgtga cctgtgcttg
    10921 tccgctcagc gtactgtcat tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg
    10981 tgactcctgc cactgggact ggaaactccc ctgcccctga ataccttgtt ttggtaaatc
    11041 ggcaccctgc aactgccagt taatttgttc aatagccgct gttaaatcat gcggataatc
    11101 agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc gattaacccg
    11161 cccggagaac tcaaatgagc cacgatgttg gggatcttgt tccatttgca ggcggatatt
    11221 gcggattgtc acctgctcgt catcttcatg ctggaacacc agaacgctgt ccgccacctt
    11281 aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca aggtattgtc
    11341 gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg tcagttggat
    11401 cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta gtggtaaaag
    11461 cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc cctgggcggt
    11521 gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac gcagtggccc
    11581 gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa
    11641 atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca cgaccaagag
    11701 tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga attagtcttt
    11761 atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt cacggcggtt
    11821 gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc
    11881 cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa tgcaaccaga
    11941 ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac gcgccaaaga
    12001 ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact ccagtgtcac
    12061 cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga gaacgatctc
    12121 atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc tcagatcgat
    12181 aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta catccaccgt
    12241 cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc cttcatcaag
    12301 ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg cacatcactc
    12361 ccttaacgcc aattacgtca agggcatacc aacttatcac tgaacggtac acaatttatc
    12421 aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg atgcggtttt
    12481 tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg aattgcagga
    12541 acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa cgcccacgct
    12601 cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta tcaacgaaaa
    12661 tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg ccttcgagaa
    12721 tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt tcaacatagc
    12781 tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc tcaagcaaca
    12841 ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct tccatcgaca
    12901 gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt tcatcaccga
    12961 cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg gaagccgatg
    13021 cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat ttagcgacct
    13081 gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat tatagggatt
    13141 tcatccgtct gataccagtc gaatagcgtt gccgcgcgct cagagttaat tgttgacaaa
    13201 gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat gttatacgca
    13261 gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa
    13321 cttttgtact agcataaaca cagaaacgaa tactggcgac ctggccttgc ggataaagcg
    13381 gtaataagca aacaatcaca gcatgtatta attgccctgc cccacccgct gcttcacctg
    13441 gtcagtttag gtttagtctc gtttatcttt acccttgtct cgcttgagct ttcgcagttt
    13501 ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc gttttatcgc
    13561 catgccgggc gcatgtggcc gggaattgcg ctgagctgtt cgctgggaaa tatcgccgca
    13621 tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat caatattgtt
    13681 gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa ccccttgcaa
    13741 aatctggctg actggctgcg tctggcattc ggcagcgcca ttgttccgcc tctgttgggg
//
LOCUS       DAEEDS010000098        13687 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEEDS010000098 DAEEDS010000000
VERSION     DAEEDS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.098"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1233)
                     /locus_tag="KLH32_003308"
     CDS             complement(join(<1..753,753..1233))
                     /locus_tag="KLH32_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HBE0140028.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSISRKGNCLD
                     NAVVECFFGTLKSECFYLD"
     gene            1609..3249
                     /gene="trg"
                     /locus_tag="KLH32_003309"
     CDS             1609..3249
                     /gene="trg"
                     /locus_tag="KLH32_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBE0140029.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(3287..4210)
                     /locus_tag="KLH32_003310"
     CDS             complement(3287..4210)
                     /locus_tag="KLH32_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0140030.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            4427..5770
                     /locus_tag="KLH32_003311"
     CDS             4427..5770
                     /locus_tag="KLH32_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0140031.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5995..7650
                     /gene="opgD"
                     /locus_tag="KLH32_003312"
                     /gene_synonym="mdoD"
     CDS             5995..7650
                     /gene="opgD"
                     /locus_tag="KLH32_003312"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBE0140032.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7790..8014
                     /locus_tag="KLH32_003313"
     CDS             7790..8014
                     /locus_tag="KLH32_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBE0140033.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            8077..8613
                     /gene="rimL"
                     /locus_tag="KLH32_003314"
     CDS             8077..8613
                     /gene="rimL"
                     /locus_tag="KLH32_003314"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBE0140034.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(8608..9588)
                     /locus_tag="KLH32_003315"
     CDS             complement(8608..9588)
                     /locus_tag="KLH32_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140035.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            9712..10704
                     /gene="tehA"
                     /locus_tag="KLH32_003316"
     CDS             9712..10704
                     /gene="tehA"
                     /locus_tag="KLH32_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBE0140036.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            10701..11294
                     /gene="tehB"
                     /locus_tag="KLH32_003317"
     CDS             10701..11294
                     /gene="tehB"
                     /locus_tag="KLH32_003317"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBE0140037.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            11597..12265
                     /locus_tag="KLH32_003318"
     CDS             11597..12265
                     /locus_tag="KLH32_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBE0140038.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(12283..12789)
                     /gene="tnpA"
                     /locus_tag="KLH32_003319"
                     /pseudo
     CDS             complement(12283..12789)
                     /gene="tnpA"
                     /locus_tag="KLH32_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12797..13687
                     /locus_tag="KLH32_003320"
                     /pseudo
     CDS             12797..13687
                     /locus_tag="KLH32_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3555 a         3213 c         3383 g         3536 t
ORIGIN      
        1 catcaagata aaaacactcc gactttaagg ttccaaagaa acactccacc acagcattat
       61 ccagacaatt gccttttctg gatatgcttt gtttaatacc atgttcttta aggatatttt
      121 gatatcttct catacgatac tgccatccct ggtcagagtg cagaacagga tgctcgtgag
      181 gattaagctt tttgaatgcc tgatcgagca tattctcaac catgttcatc actggtcttt
      241 ccgaaaggct gtaagaaata acttcgttgt tgaagagatc tattactgga gacaaataca
      301 gcttgcgccc attgactgca aattcagtaa catcggtaac ccacttctcg tttggccgcg
      361 tagccttgaa atctctttgg agaacattag gggcggtttg ccctacctct cctctgtaag
      421 agcggtatcg cttgacctta attgctgctt taagtgagag ggttcccatc aggcgctgaa
      481 cagctttatg gttaatctgt ttcccttctc gatgaagaga cagcgttacc ctacggtatc
      541 cgtatcggcc tctattctcg tgataaatct caccaatacg ctttttaacg tccgcatact
      601 tgtcaggctt gctgagagcc tttagatggt aataaaacgt actgcgcggt atctccgcag
      661 ccctgagaag ctcatcaaga ggataaaact gccttagctc gttgagtact ttcacttttt
      721 cgtgggatga gctaaggctt tcagcttttt tagatacata agccgcgttt caagaaatcg
      781 aacttgcctt tcaagatcct caatgcgtcg gtcttttgac agctccaatg ctgatgccgc
      841 tttttctgga tcaactgata ttgcaatgtt tcttttggtg ccaatcttga gcgcgcgtaa
      901 accagcttct ccgcgctctt catagacctt cagccacctg gctacagaac cactaccagc
      961 aagcataaag tgagcagcag cctgattaag ggacatgtgc tgctcgatca cagctttcac
     1021 gaccttaata cgcaactctg gatcagcact aacgccttta ggtttgggaa ttaaaccttt
     1081 ttctccatgt ttttcataga gggcaaccca tgtcctgacc tgggttcggg ggacaccaaa
     1141 acgtgccgag atgatcctgt aaccatcatc agtagtgaag tagtgattca cgacttcaag
     1201 gcgcttttca aaagggtatt ttggctttga catattaggg gctattccat ttcatcgtcc
     1261 aacaaaatgg gtgcagtaca gttgatagaa atatcggtcg gggccttcgt ctttctgatt
     1321 cccggttagc ctgaaaacag aaagtctcag gcacccgcag gcatcctatg aggtttcctt
     1381 agggacgaaa ataatcactt cacgaaattg cgtgctgttt tccagaattt ttcgtcattc
     1441 gggttagcca gtttagccat tcgttactct cttcattcca atagcattaa ttttctctgc
     1501 aataattgtt gtaaaaatgt gacgcaaaga ggtttttggt cataagtaat taccgtcaag
     1561 tgccgatgac tttctatcag gagtaaacct ggacgagaga caacggtaat gaatacaact
     1621 ccctcacagc gattaggttt tttgcatcac atcaggctgg ttccgttatt tgcctgcatt
     1681 cttggcggta tcttagttct attcgcatta agttctgctc tggctggcta tttcctctgg
     1741 caggccgatc gcgatcagcg tgatgttact gcggagattg agattcggac cgggttagcg
     1801 aacagttcag attttttgcg ttcagcccgg atcaatatga ttcaggccgg ggctgcgagt
     1861 cgtattgcgg aaatggaagc aatgaagcga aatattgcgc aagccgaatc ggagattaaa
     1921 cagtcgcagc aaggttatcg tgcttatcag aatcgaccgg tgaaaacacc tgctgatgaa
     1981 gccttcgaca ctgaattaaa tcaacgcttt caggcttata tcacgggtat gcaacctatg
     2041 ttgaaatatg ccaaaaatgg catgtttgaa gcgattatca atcatgaaag tgagcagatc
     2101 cgaccgctgg ataatgctta taccgatatt ttgaacaaag ccgttaagat acgtagcacc
     2161 agagccaacc aactggcgga actggcccat cagcgcaccc gcctgggtgg gatgttcatg
     2221 attggcgcgt ttgtgcttgc cctggtcatg acgctgataa catttatggt gctacgtcgg
     2281 atcgtcattc gtccactgca acatgccgca caacggattg aaaaaatcgc cagtggcgat
     2341 ctgacgatga atgatgaacc ggcgggtcgt aatgaaatcg gtcgcttaag tcgtcattta
     2401 cagcaaatgc agcattcact ggggatgaca gtagggactg ttcgacaggg cgcggaagag
     2461 atttatcgtg gcaccagcga aatttcagct ggcaatgcgg acctgtcatc tcgcaccgaa
     2521 gaacaagcgg cggctatcga acaaactgcc gccagcatgg agcaactcac tgcgacggtg
     2581 aaacagaatg cggataacgc gcatcatgcc agcaaactgg cgcaagaggc ttctattaaa
     2641 gccagcgatg gcgggcagac ggtttccggt gtagtaaaaa cgatgggcgc tatctccacg
     2701 agttcgaaga aaatttctga gatcaccgcc gtcatcaaca gtattgcttt ccagacgaat
     2761 attctggcac tgaatgctgc cgttgaagcc gcgcgagcgg gtgagcaagg gcgtggattt
     2821 gccgttgtcg ccagcgaagt acggacactc gcaagccgca gcgctcaggc agcgaaagag
     2881 attgaaggct tgatcagtga atcagtcagg ttaattgacc tggggtcgga tgaggtggca
     2941 acggcaggga aaaccatgag cactattgtt gatgccgtcg cgagtgtcac acatatcatg
     3001 caggaaatcg ccgccgcctc ggatgaacaa agtagaggca taactcaggt tagccaggcg
     3061 atttctgaaa tggataaggt gacgcaacag aatgcttctc tggtagaaga ggcctcagca
     3121 gcggcggtgt cccttgaaga acaggctgca cgattaactg aggcggtgaa cgtattccgt
     3181 ctgaacaaac attctgtgtc ggcagaacct cgcggagcgg gtgaaccagt tagtttcgct
     3241 acggtgtgaa aatgttcaag gagggatcga cagatccctt cacctttcag aacggcattg
     3301 attttcgaat agcgttaatc atcaactggc aaccagaaga gaacgtcgca tctacgcggg
     3361 tcagtattcc aatcggttcg cctgcaccat gtcccggaac aggcagggcc accagcgtgg
     3421 catgacgcag gtcgtctttt acagcgccag aagggacaaa ccacacgtaa tcgtattcaa
     3481 cggtaagttg acgagatagc gaagtagaca gcgtttcgat acaacccgaa ggaattttac
     3541 agccctggct ctgcactaat gcatctgaat gctggcgtgg cgcagtgcct tctggtgata
     3601 caacgaccgg ccattccagc acccggctta gcgttacgtt ctcctgaagt agcgggtgat
     3661 tagggcggac aaccagcttc aacgattcaa gaaacagcag ttcgtaatta agcccggtca
     3721 tcagttcagg atctgacatc cgaccaatgc cgatatcgat ttccccggtt tttaaacccg
     3781 ccagaatcat agggttactc attgtcgcaa cttgcaaggt cgtctctttt tgttgctgat
     3841 gaaactgacc tataaccgaa ggtaatatcc ccagtgccgc agtaggtagt gcaccaaccc
     3901 tgacgacatc attattaaga ccttctttac gatgaagcga ctgtccggca gtgttgatgg
     3961 cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc gggtaaggta agttgcgccc
     4021 cctgacgacc acgctcaaac aagcgagcgc ccgtcagctg ctccagttca ttcaatgtat
     4081 tagagagcgc aggttgactc aaattaaggg tttcagctgc gcgccccaaa gttccttgtt
     4141 gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat gcgctgacta aacagactat
     4201 ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct gacaagtgaa gttgtttgat
     4261 tatgataact tgattgcaaa atattattaa taattaaagc aattatgtta cagcaaaatg
     4321 gataatattg atgttttcgc ggcgagatca cagtttgtaa attcttcccg ccagagtgaa
     4381 tgcggttacc tacactccag attactgacc actggaggca gacactatgg cgaacagcat
     4441 cacggcggat gagattcggg aacagttttc gcaggcaatg tcagccatgt accagcaaga
     4501 agttccgcaa tatggcacgc tgctggaact ggtagctgat gtgaatctgg ctgtgctgga
     4561 aaacaatcct caactgcacg aaaaaatggt aaatgcagac gagctggcgc gactgaatgt
     4621 tgaacgtcat ggggcgattc gcgttgggac tgcacaagag cttgctactc ttcggcggat
     4681 gtttgccatt atggggatgt acccggtgag ctattacgat ctctcgcagg caggggtgcc
     4741 ggtacattcg acagcatttc ggcccattga tgatgcttct ctggcgcgta atcccttccg
     4801 cgtttttaca tccttactcc gccttgagct tatcgagaac gaaattttgc gccagaaagc
     4861 ggcggagatt ctgcgtcagc gcgatatctt caccccacgt tgtcgacaac tgttagagga
     4921 atatgagcag cagggcggtt ttaacgaaac acaggcacag gagtttgtgc aggaagccct
     4981 ggaaacgttt cgctggcacc agttagcaac ggtagatgaa gaaacctatc gcgccttgca
     5041 caacgaacat cggttgattg ctgatgtggt ctgttttcct ggatgccata tcaaccacct
     5101 gacgccacgt acgctggata ttgaccgggt gcagtcgatg acgcctgaat gcggaattga
     5161 acccaaaatt ctcatcgagg ggccgccgcg ccgcgaggta ccgattttac tacgccagac
     5221 cagctttaaa gcactggaag agacggtgtt gtttgcgggg cagaaacagg gcacgcatac
     5281 cgcgcgcttt ggtgaaattg agcagcgtgg cgtggcatta acgccgaaag ggcgacaact
     5341 gtatgatgat cttctgcgta acgctggaac cgggcaggat aatctcactc accaaatgca
     5401 tttacaggaa accttccgca cttttcctga cagtgagttt ttaatgcgtc agcaagggtt
     5461 ggcatggttc cggtaccgtc tgacgccttc gggtgaggcg catcgtcagg cgattcatcc
     5521 tggagacgat ccacagccct taattgaacg tggttgggta gtggcgcaac ccatcaccta
     5581 tgaagatttc ttgcccgtta gcgcggcggg gatcttccag tcaaatctgg gtaatgaaac
     5641 gcagacacgc agtcacggta atgccagtcg cgaagcattt gagcaggcgt tgggttgtcc
     5701 ggttttggat gagttccagc tttaccagga agcggaagaa cgcagtaaac gtcgctgtgg
     5761 tttgctttaa attctgacca tccgcctttg caaaaatttg cctgatttta caaacgaatc
     5821 aggctcatcc catcgacata aaaaaaatgc cgatttatgc atattctctc agttcaacaa
     5881 ttggattatt aataaatatt gtctagagtg agcggtcata aataagcact ttcttgccgc
     5941 tgaaaacgac cagcgcggga ccattcacaa caccagaagg actcactttc aggtatggat
     6001 cgtagacgat ttattaaagg tttaatggct atggccgccg tgtgcggtac cagcggcatt
     6061 gcttctcttt tttctcaggc agcattcgcg gcagattctg atattgccga cgggcaaacc
     6121 cagcgttttg acttctccat tctacagtca atggcgcacg acttagcgca aacagcgtgg
     6181 cgtggtgcgc ctcgtccgtt acctgacacg ctggcgacaa tgacgccgca ggcttataac
     6241 agtattcaat acgacgccga aaaatcgctc tggcataacg ttgagaaccg tcaactggac
     6301 gctcagttct tccatatggg aatgggattc cgtcgccgcg ttcgtatgtt ttctgtagat
     6361 ccagcaacac atctggcgcg tgaaattcac tttcgcccgg agttgttcaa atacaacgat
     6421 gcaggtgttg ataccaaaca attagaaggg caaagcgatc tcggctttgc cggttttcgc
     6481 gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat catttctcgg cgcgagttat
     6541 ttccgcgccg ttgatgatac atatcaatac ggtttgtcgg cccgcggcct ggcgatcgac
     6601 acttacaccg acagtaaaga agagttcccc gactttaccg ccttctggtt tgatacggta
     6661 aaaccggggg caactacctt taccgtttat gcgttgctcg atagcgccag cattactggt
     6721 gcctataagt tcactatcca ttgtgagaaa agtcaggtga ttatggatgt ggaaaatcac
     6781 ctgtatgcgc gcaaagacat taaacagctg ggcatttcgc cgatgaccag tatgttcagc
     6841 tgcggtacta atgaacgtcg gatgtgcgat acaattcatc cgcaaattca tgactctgat
     6901 cgtctgtcca tgtggcgggg caacggcgag tggatttgcc gtccgctgaa taatccgcaa
     6961 aaattgcagt tcaatgctta caccgacaac aacccgaaag ggtttggttt attgcaactg
     7021 gatcgtgact tctcccatta tcaggacatt atgggctggt ataacaaacg cccaagtctg
     7081 tgggtggaac cgcgtaacaa gtggggtaag ggcaccatcg gcctgatgga aatcccaaca
     7141 acgggcgaaa cgctggataa cattgtctgc ttctggcagc cagaaaaagc tgtaaaagca
     7201 ggtgatgagt ttgcattcca gtatcgtctg tactggagtg cgcaaccgcc tgttcattgc
     7261 ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg gtttcccgga aggttgggcg
     7321 ccaggtgaac actatcccga aaaatgggcg cgtcgttttg ccgtcgattt cgttggtggt
     7381 gatctgaaag ctgccgcgcc aaaaggcatt gagccggtga ttacgctttc cagtggggaa
     7441 gcgaagcaaa tcgaaattct ctatattgaa cccatcgatg gttatcgtat tcagtttgac
     7501 tggtatccga cttcggactc cactgatccg gtcgatatgc ggatgtatct acgttgtcag
     7561 ggggacgcta tcagtgaaac atggctgtat cagtatttcc cgccagcgcc ggataaacgt
     7621 cagtatgttg acgaccgcgt gatgagttaa tcgttttttc ttcggcacct tcttcgggag
     7681 gtgccgtctg gttaaacacg atcccgctcg catttttccc taagttaaat gagtaatctg
     7741 atggtgtgta tttcagatac accgtgtcag ccactaacag ggagtgcgta tgtttccaga
     7801 ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct cgctttatgt ccttgttcga
     7861 taaacacaat aaacttgatc atgaaattgc cagaaaggaa ggttccgacg gtcgagggta
     7921 caatgcggaa gtggtccgca tgaaaaaaca aaagctacag ttaaaagatg agatgctcaa
     7981 aatcctgcag caggagagcg tcaaagaggt gtaaactttc ctaagccgcc agccaggcgg
     8041 cttttttaac aacagcatgg attgactgga gataagatga ctgaaacgat aaaagtaagc
     8101 gaatcacttg aattacatgc tgttgcggaa agtcacgtca cacctcttta tcagttaatc
     8161 tgtaaaaata aaacctggtt acagcagtcg ctaaactggc cgcagttcgt tcagagtgaa
     8221 gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc atcaacgcgg ctatgccaaa
     8281 atgttcatga ttttcaaaga agatgaactt atcggcgtta tctcgtttaa tcgtattgaa
     8341 ccactgaata aaactgctga aatcggctac tggctggacg aatctcatca ggggcagggg
     8401 atcatttctc aggcgctgca ggcattgatt catcattacg ctcagtctgg tgaacttaga
     8461 cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca accaggtcgc tttgcgcaat
     8521 ggttttatcc ttgaaggttg cctgaaacag gctgagttcc tgaatgatgc ctatgatgat
     8581 gtgaacttat acgcgcgtat tatcgattca taataaccct gccagcggcg ttcgcgtaat
     8641 gcgctcctcg ccgttaatga cttttggacc acggacatga accgtgtcgc catcaaaggc
     8701 ttcaacgacg gcgtggtcgg ttaactccac atggttttca attatcaccg cacctgtaat
     8761 acggctctca ccttgtatta caacgtgctc gtcgagcaga atcggtccac cacgtaccac
     8821 ggcatttccg ccaatcaaga catgatgttt taacacacaa ttaccttcca caatggcata
     8881 ttccgccacc tgcgaactgt aatgaatcgt cggaatggca tcttcttcta tgccagcttt
     8941 cacctgcgcg tgaccgtaga ctttagcgca atcgcatagc cagacgttgt tctcttcatt
     9001 accttcaaca ctggcaaaat caaaaacttc ggcgcgatgt tcaataaaag cataccggac
     9061 gacggcatcg ccataaattt gtgcctggtg gacaatacgc gaggcgctaa cccttgcgcg
     9121 atcataaatt tggagcagga gttgatggtc gggcgttaag ccttgtgcgg cgacgatcat
     9181 agaatgttga tcaattaagg cgtgaccaaa tattctacat tgcacgtaaa ctaatgagtc
     9241 atgaatggtg acactgtcac ttatatatgc accctggctg atttcactat tatcgatcca
     9301 gacgttatcc gttgcgtaaa cctctcccca cagcacgctg gtgccggtga tacgcgtatt
     9361 accggaaatc accgttccgc cgaacgcaat agcattctgg tcgtaaatcc agcaatttcc
     9421 ttcctgtgct aacaccgttt cgcgatcgat ccagccgcca gcggttcctg ctatcacatc
     9481 gttaaaatcg ctaatggcga taatctgccg taataacaca ctttttttag tgccatcttc
     9541 ctgataacta aaggctcgct gttcttcact taggcgatat ttgcgcatag cgttttccca
     9601 caggtgactt actataaccg tagcaaattc tgcggctctg gctatgctcg agaaaattcc
     9661 ataaaatgca tttcaaatat actttataaa ttaaacaaaa tgagtaagaa gatgcagagc
     9721 gataaagtgc tcaatttgcc ggcaggctac tttggtattg tgttggggac gatagggatg
     9781 ggatttgcct ggcgctatgc cagccaggtt tggcaggtca gccactggtt aggggatggg
     9841 ctggtgattc tggcgatgat catctgggga ttattgacta gcgcatttat tacccgactc
     9901 atacgctttc cgcatagcgt gctggcggaa gttcgccatc cagtgctgag cagttttgtg
     9961 agtttgtttc cggcaacgac gatgctggtg gcgattggtt ttgttccgtg gtttcgccca
    10021 ctggcggtgt gcatgttcag ttttggtgtc gtggttcagt tggcttatgc cgcctggcaa
    10081 actgcgggat tatggcgcgg atctcaccct gaagaagcta ccacgcctgg actgtatctg
    10141 ccgacagttg ccaacaactt tatcagcgca atggcctgtg gtgcgttggg ctacaccgac
    10201 gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc taagccttga accggtgatc
    10261 ttgcagcgtc tgcgcagttc gggagaatta cccacggcac tgcggacatc actcggcatt
    10321 cagctcgctc ctgcgctggt ggcctgtagt gcctggctga gcgtcaacgg cggcgagggt
    10381 gacacgctgg cgaaaatgct tttcggttat ggactgctgc aactgctgtt tatgctacgt
    10441 ctgatgccat ggtatctctc ccagccattt aatgcttcat tctggagttt ctcgttcggc
    10501 gtatctgcac tggcaaccac cggtttgcat ctggggagtg gcagcgataa tggatttttc
    10561 catacgctgg cggtgccgct gtttatcttt accaatttta ttattgcaat actgctcatc
    10621 cgtacttttg cgcttctgat gcagggaaaa ttgttagtca gaaccgagcg cgccgtttta
    10681 atgaaagcag aggacaaaga atgatcattc gtgacgaaaa ctattttact gataaatatg
    10741 aattaacccg cacacactct gaagtactgg aagcggtgaa agtggttaaa ccgggtaaaa
    10801 cgctggatct gggctgtggc aatggtcgta acagtcttta cctggcagcc aatggttatg
    10861 atgttgacgc atgggataaa aatgccatga gtatcgccaa cgtcgagcgc attaaatcca
    10921 ttgaaaatct ggataattta cacacccggg tcgttgatct gaataacctc acatttgatg
    10981 gacagtacga ttttattctt tcgactgtgg tgctgatgtt ccttgaggct aaaaccatcc
    11041 cagggttgat tgccaatatg caacgttgca ctaaacctgg tggttacaac ctgattgtgg
    11101 cggcgatgga taccgctgat tatccatgta ccgtcggctt cacgtttgcc ttcaaagagg
    11161 gagaattacg tcgatattac gaaggctggg agagggtgaa atacaatgaa gacgtcggcg
    11221 agctgcaccg caccgacgcc aacggtaatc gtattaaact gcgtttcgcc acgatgctgg
    11281 cacgtaaaaa atgacccggt aagcacaaaa cgcgtgaaaa ttccccacgc tgagatgatt
    11341 tactgttctt cttttcggta agcatatttt ttatcgaagg gatgtgaaat taatcacagt
    11401 agtcgaagtt ttttagcagc ttaacttact gaaatttaac tactgatgat tgacttagcc
    11461 cctttttcgg cattgactat gtcgtctgaa aaggggctga aaaatttatt ttcaccaaca
    11521 ctttttttgc cacaacacga agcggcgctt tttgctataa cttagaaagt aatataatca
    11581 tctcaggaaa ctattcatgc gtaccacatc atttgcgaaa gttgcagctt tatgcggctt
    11641 gttggctctg tctggttgtg cctctaaaat cacccagcca gataaatatt ctggtttttt
    11701 aaacaattac tctgatttaa aagaaacaac ctcagcaaca ggtaaacctg ttttacgttg
    11761 ggtagacccg agttttgatc aaagcaaata tgacagcatc gtctggaacc caatcactta
    11821 ttatccggta ccaaaacctt ctacccaggt agggcagaaa gttctggata aaattttgaa
    11881 ctataccaac accgaaatga aagaagcgat agcgcagcgt aaaccagtgg ttaccaccgc
    11941 tgggccgcgt agtctgattt tccgtggggc cattaccggt gtagatacca gcaaagaagg
    12001 gctgcaattc tatgaagtgg ttcctgttgc attagtggtt gcggggacgc aaatggctac
    12061 aggccaccgt accatggata ctcgcctcta ttttgaaggt gagctgattg atgcagcgac
    12121 taataaaccg gttatcaaag tcgttcgtca gggcgaaggt aaagacctga ataacgaaag
    12181 tacgccaatg gctttcgaaa atattaaaca agttattgat gacatggcga ccgatgccac
    12241 catgtttgac gttaacaaaa agtagttcca gacgcgccat cgttagatgg cgctttttat
    12301 ccggtgcgcc gtaaaacccc atccttcagg gcggggatat aaggcgcggt tttccacctg
    12361 accaggtgtt tgctgttgtt caatgtattg gcggatgacg gatattggcg cacctccgca
    12421 gctactggca aaatagccag gactccacaa aacgcctttg tagtaatacc tgactgcaat
    12481 atctggtcga tctcgtcgca gtaacctacc cgatacgcct ttgaggctgt ttaccagact
    12541 ggatatcgcc agtttgggag gttagttaat taacaaatgg acgtgatctg gttcgccatc
    12601 catttcaacc agttcagctt caaaataagc acatacattt gaaaagtaag tgcgtagttt
    12661 ttctgtcgcg tcatggtcaa aaatctggcg tcggtatctg gtgacaaaga ctaggtgaac
    12721 atgcatcagg aaaacacaat gccttccacg ccggatatcg gtttcttttt tcacagacca
    12781 aagtatcatt tgacctgtga aacgattaca ggcatttaaa ttccagttaa gaccaggtgg
    12841 tcaacaggag tgtgaaatga ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc
    12901 tctggcacgt cagaatgaga atcatgaggc cggtaataaa tacatccctt acgggaaaat
    12961 ggcttcctgg ctggttgagt ggaaaaatgc cactgaaacg caatggctta aagattctcc
    13021 ctcacagcca ttgcaacagt cactgaaaga ccttgagcgg gcttacaaaa acttcttccg
    13081 gaagcgggct gcttttcccc gattcaaaaa gcggtgacag aatgatgcat tccgctaccc
    13141 gcagggcgtt aagctcgatc aggaaaacag ccgtattttt ctgccgaaac tgggctggat
    13201 gcgctaccgg aatagccgtc aggtcacggg tgttgtgaaa aatgtcactg tcagccagtc
    13261 ctgcggtacg tggtacatca gtattcagac agaaagtgaa gtatccactc cggctcaccc
    13321 ttcagcatca atggtcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg
    13381 cacagtcttt gagcctgtaa acagttttca gaaaaaccag aagacgctgg cgagacttca
    13441 gcgacagtta agccgcaagg tcaaattcag caacaactgg cagaagcaga aacgcaaaat
    13501 acagcgactg cattcctgta tcgcaaatat ccgcagggac taccttcaca aagtcacaac
    13561 ggccgtcagc aaaaaccacg caatgatagt cattgaggat ttgaaggtca gcaacatgtc
    13621 aaagtcagca gcgggtacgg tcagccagcc ggggcgcaat gtccgggcaa aatcaggttt
    13681 aaaccgt
//
LOCUS       DAEEDS010000099        13669 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEEDS010000099 DAEEDS010000000
VERSION     DAEEDS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.099"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(337..582)
                     /locus_tag="KLH32_003321"
     CDS             complement(337..582)
                     /locus_tag="KLH32_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140039.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="KLH32_003322"
     tRNA            complement(1227..1302)
                     /locus_tag="KLH32_003322"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="KLH32_003323"
     CDS             complement(1408..2115)
                     /locus_tag="KLH32_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBE0140040.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="KLH32_003324"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="KLH32_003324"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBE0140041.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KLH32_003325"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KLH32_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBE0140042.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KLH32_003326"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KLH32_003326"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBE0140043.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="KLH32_003327"
     CDS             complement(7299..7574)
                     /locus_tag="KLH32_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBE0140044.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KLH32_003328"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KLH32_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBE0140045.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="KLH32_003329"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="KLH32_003329"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBE0140046.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="KLH32_003330"
     CDS             9534..9860
                     /locus_tag="KLH32_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBE0140047.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="KLH32_003331"
     CDS             10044..10763
                     /locus_tag="KLH32_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBE0140048.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="KLH32_003332"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="KLH32_003332"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBE0140049.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="KLH32_003333"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="KLH32_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..13589)
                     /locus_tag="KLH32_003334"
                     /pseudo
     CDS             complement(13164..13589)
                     /locus_tag="KLH32_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3515 a         3263 c         3539 g         3352 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggacatgccg gtaatgctgc caacttactg atttagtgta
    13621 tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtc
//
LOCUS       DAEEDS010000100        13532 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEEDS010000100 DAEEDS010000000
VERSION     DAEEDS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.100"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..890)
                     /locus_tag="KLH32_003335"
     CDS             complement(<1..890)
                     /locus_tag="KLH32_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0140050.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="KLH32_003336"
     CDS             1131..1931
                     /locus_tag="KLH32_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE0140051.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="KLH32_003337"
     CDS             2009..2779
                     /locus_tag="KLH32_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0140052.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KLH32_003338"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KLH32_003338"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBE0140053.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KLH32_003339"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KLH32_003339"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBE0140054.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="KLH32_003340"
     CDS             5046..5768
                     /locus_tag="KLH32_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0140055.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KLH32_003341"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KLH32_003341"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBE0140056.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="KLH32_003342"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="KLH32_003342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBE0140057.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="KLH32_003343"
     tRNA            7161..7237
                     /locus_tag="KLH32_003343"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="KLH32_003344"
     CDS             complement(7368..8414)
                     /locus_tag="KLH32_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBE0140058.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KLH32_003345"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KLH32_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KLH32_003346"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KLH32_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBE0140059.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KLH32_003347"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KLH32_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBE0140060.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="KLH32_003348"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="KLH32_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KLH32_003349"
                     /pseudo
     CDS             complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KLH32_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3522 c         3611 g         3298 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccgtccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct gaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt ggtttcattg tg
//
LOCUS       DAEEDS010000101        13019 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEEDS010000101 DAEEDS010000000
VERSION     DAEEDS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.101"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..319)
                     /locus_tag="KLH32_003350"
     CDS             complement(<1..319)
                     /locus_tag="KLH32_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBE0140061.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="KLH32_003351"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="KLH32_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KLH32_003352"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KLH32_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBE0140062.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KLH32_003353"
                     /pseudo
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KLH32_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="KLH32_003354"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="KLH32_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBE0140063.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="KLH32_003355"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="KLH32_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBE0140064.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="KLH32_003356"
                     /pseudo
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="KLH32_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="KLH32_003357"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="KLH32_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="KLH32_003358"
     CDS             10613..11503
                     /locus_tag="KLH32_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBE0140065.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="KLH32_003359"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="KLH32_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBE0140066.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12493..12569)
                     /locus_tag="KLH32_003360"
     tRNA            complement(12493..12569)
                     /locus_tag="KLH32_003360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13019
                     /locus_tag="KLH32_003361"
     CDS             12812..>13019
                     /locus_tag="KLH32_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBE0140067.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLK"
BASE COUNT         3187 a         3373 c         3307 g         3152 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccacttct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aagggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg tgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctagcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct agcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat accctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcactgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagtaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctggcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgag aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctgaaaa
//
LOCUS       DAEEDS010000102        12907 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEEDS010000102 DAEEDS010000000
VERSION     DAEEDS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.102"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KLH32_003362"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KLH32_003362"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBE0140068.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="KLH32_003363"
     CDS             complement(1806..3224)
                     /locus_tag="KLH32_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBE0140069.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="KLH32_003364"
     CDS             complement(3378..3722)
                     /locus_tag="KLH32_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBE0140070.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KLH32_003365"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KLH32_003365"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBE0140071.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="KLH32_003366"
     CDS             complement(4712..5647)
                     /locus_tag="KLH32_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBE0140072.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="KLH32_003367"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="KLH32_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBE0140073.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="KLH32_003368"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="KLH32_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBE0140074.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KLH32_003369"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KLH32_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBE0140075.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="KLH32_003370"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="KLH32_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBE0140076.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="KLH32_003371"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="KLH32_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBE0140077.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="KLH32_003372"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="KLH32_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBE0140078.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KLH32_003373"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KLH32_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBE0140079.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="KLH32_003374"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="KLH32_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBE0140080.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="KLH32_003375"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="KLH32_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBE0140081.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3400 a         3296 c         2951 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DAEEDS010000103        12874 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEEDS010000103 DAEEDS010000000
VERSION     DAEEDS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.103"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="KLH32_003376"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="KLH32_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBE0140082.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="KLH32_003377"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="KLH32_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBE0140083.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="KLH32_003378"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="KLH32_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBE0140084.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..3249
                     /locus_tag="KLH32_003379"
     CDS             1993..3249
                     /locus_tag="KLH32_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBE0140085.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3542..4783
                     /gene="agp"
                     /locus_tag="KLH32_003380"
     CDS             3542..4783
                     /gene="agp"
                     /locus_tag="KLH32_003380"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBE0140086.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4821..5048)
                     /locus_tag="KLH32_003381"
     CDS             complement(4821..5048)
                     /locus_tag="KLH32_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBE0140087.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="KLH32_003382"
     CDS             complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="KLH32_003382"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE0140088.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6038..6145
                     /locus_tag="KLH32_003383"
     CDS             6038..6145
                     /locus_tag="KLH32_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140089.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="KLH32_003384"
     CDS             complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="KLH32_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBE0140090.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="KLH32_003385"
     CDS             complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="KLH32_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBE0140091.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(8081..8671)
                     /locus_tag="KLH32_003386"
     CDS             complement(8081..8671)
                     /locus_tag="KLH32_003386"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBE0140092.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="KLH32_003387"
     CDS             complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="KLH32_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBE0140093.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="KLH32_003388"
     CDS             complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="KLH32_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBE0140094.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="KLH32_003389"
     CDS             complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="KLH32_003389"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBE0140095.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="KLH32_003390"
     CDS             complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="KLH32_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBE0140096.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11958..12596
                     /gene="rutR"
                     /locus_tag="KLH32_003391"
     CDS             11958..12596
                     /gene="rutR"
                     /locus_tag="KLH32_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBE0140097.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3279 a         3238 c         3277 g         3080 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgactg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccgcgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagcctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag
     2461 tattgtttag atgatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata
     2521 tctgatttag atactggtat tgatttgtct ggcatatctt ttattcagag tgaaattaat
     2581 aaaacatatg gattaaaata cgctccagta gatggcgatg gatattgtct gttaagagcc
     2641 atactggttt taaaagaaca tgagtattca tgggcgctgg gcagccataa gacgcaaaag
     2701 caagtatacg aagagtttat taaaattgtt gataaacaaa cgatcgaggc tcttgttgat
     2761 acggcattct ataatctcag ggaagatgta aagacattat ttggcgttga tctgcaatct
     2821 gacaacaaaa ttcagggtgt gggtagtttt atgtcatgga gctttctgtt ttttaaaaaa
     2881 caattcattg atagttgctt gaatgacaaa aaatgcatac tgcatttacc cgagtttata
     2941 tttaatgata acaagaattt gcttgcttta gataccgaca cgtcggatag gattaaagcg
     3001 gtgaagaatt ttcttgctgt tctttcagat agcatttgct cattatttat tgttaatagt
     3061 aatgtggcat caatctcctt gggaaatgaa tccttttcaa cagatgaaga tcttgagtat
     3121 ggttatttaa caaacactgg caatcattat gatgtttacc tccctcctga acttttttct
     3181 caggcttata agttaaaaaa taaggaaatg aatgcgcaac ttgactattt aaatcgttat
     3241 gcaacttaat ggcaaaggca tatgctaaaa accattgtta ttagtctcac acttttttat
     3301 tggtaaatat tgtctctgta ttggtaacgc cgcagatatt ctgtttagcc acaggtgcaa
     3361 ttatcagcgg cgtacgcgag gcaggggcta atcaggcata gtttgcgtca aaccttgcct
     3421 gtttttgaag atgtatatag aaaaacaggc gttcaacaag ccattttgcg aacctgttcc
     3481 cggaaaaaag tcatatttct gtcacactct ttaatgattg ataacaaaag aggtgccagg
     3541 aatgaacaaa acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc
     3601 tcaggcacaa accgtaccgg aaggctatca gctacagcaa gtgctcatga tgagccgcca
     3661 taacttacgt gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga cgccgaataa
     3721 atggcccgaa tgggacgtcc ccggtgggca actcaccacc aaaggtggcg tgctcgaagt
     3781 gtatatgggc cattacatgc gtgaatggct ggcagagcag gggatagtga aatcggggga
     3841 atgcccgccg ccggacaccg tttatgccta tgccaatagt ctgcaacgta ccgtcgctac
     3901 cgcccagttc tttattactg gtgcattccc tgggtgtgat attcctgtgc atcaccagga
     3961 aaaaatgggc actatggacc caacctttaa cccgatgatc accgatgatt ccgccgcatt
     4021 cagtgaacag gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag
     4081 ctaccagcta ctggaaaaaa tcgttaacta taaagattcc cctgcctgta aagagaaaca
     4141 acagtgttcg ctggtggatg gcaaaaatac ctttagcgcc aagtatcaac aagaaccagg
     4201 tgtttccggg ccgctgaaag tcggcaactc gctggtagat gcgtttactt tgcaatacta
     4261 cgaaggtttt ccgatggatc aggtggcctg gggagaaatc aaatctgacc agcagtggaa
     4321 ggtattgtcg aagctgaaaa acggttacca ggacagtctg tttacctcac cggaagtggc
     4381 gcgcaatgtt gcgaaaccgc tggtcagtta tatcgacaaa gccctggtca ccgatcgcgc
     4441 cagtgctccg aaaatcacgg tgttagttgg gcacgactcc aacatagcct ctctgttaac
     4501 ggcgctggat ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg
     4561 caaaatcgtt ttccagcgtt ggcatgacag caaagccaat cgcgatttga tgaaaattga
     4621 atatgtgtac cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc
     4681 tgcgcagcgc gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttctgccc
     4741 gatggataag tttgatagcg tgttgaatga ggcggtgaaa taacagaaaa ctcccccgcg
     4801 agaagcgggg gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa
     4861 agcgaatctt tatcggtctt agcaaaggct ttgactaaca cttcatcact accttcttcc
     4921 caaatctttt ctgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt
     4981 tccggcgacg tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga
     5041 gttggcatgc gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga
     5101 catattcccc ttgataacga gcaatagaca gttcttcctg gcttggctgg cgtgagccgt
     5161 caccgcctgc gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa
     5221 ataattcctg cgctgcgtag ccaatgggga caatcaccat gccgtgatgc gcaagggtcg
     5281 tccaggtgga tgtgatggtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc
     5341 tcgccagttt tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg
     5401 tacgcatttg accggacatg ttgccaaagc gggtaggtgt accaaaaata atggcgtcgt
     5461 aatcggccag ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt
     5521 caaataattg cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcca
     5581 ctttgcttgc accctcagcg actgcgcgtg ccatcgtttc aatatgtccg tacatggaat
     5641 aataaagcac cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag
     5701 cgattcgcta ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc
     5761 acttatttga tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt
     5821 ttatctcatc gcgttttttt aatcataaga gcggcttatg gataattatt ggagatgata
     5881 tctattctcg ctaagaagct gttgcaggat attaccaaac gcgggtctgt ccgcgtcagt
     5941 tcactaagct tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct
     6001 atgcaatatg atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc
     6061 cggcaatttt gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggcaagag
     6121 cagtcacggc aaaagcgaca actagccggg ctaatcaatg acgaatgcat ttttttctta
     6181 agctcgtcaa aaagtcatca ccgccggtaa cccggcggtt ttctgtttat ggctcctgat
     6241 gaacaacttc tggcggtgga acgtcaacca accttctgct taataacgca ttgagtaaaa
     6301 tcgcgccaaa ggttgctgta ccgatccctc ccaacgtaaa accgccaagc gtgagagcaa
     6361 aatcacccgc gcccagcacg aaggttactg cgaccataat caaattagcg ttctggctta
     6421 aatcgacacg gttttgtacc catatccttg cgcctgcgac ggcaatcagc ccgaacacaa
     6481 caattgatgc accaccaatc accgcggccg gaatggtatg gatcagcgcg ccaaatttcg
     6541 gcgaaaagcc caacagcata gcgatgacgg cagctgcaac aaacaccagc gtcgagtaga
     6601 cttttgtcac ggccatcaca ccgatatttt cagcataggt ggtgacgccg ctaccgccga
     6661 cagagccgga aagcatcgtt gccagaccat caccgacgaa tgctcgcccc atatacgggt
     6721 ccatattgcg tccggtcatc ccggcgaccg ccttgagatg acctaagttt tccgccacca
     6781 gaatcaccgc cacgggcgca atcaacatca tcgcttggcc attaaaacca ggagtggaaa
     6841 aatgcggcag accgaaccag gcagcatagc tgacgagagt aaaatcgacg gcttttccca
     6901 gccctaaaac gttggtcatc acgccataca gcagacaggc gacaattaat cctacgagaa
     6961 tcaataaccg ctggatcatg ccacgggtaa acaccgccac cagcccaata cacagcaccg
     7021 tcattaccgc catccagcta tcaaaggccg aagccgatac acttttcact gcgataggcg
     7081 ctaagttcag gccaatcgcc atcaccaccg cacccgtcac caccggcggc attagtcgtt
     7141 caatccagcg tgtaccaatt ttcatcacca ccaggccaat gacggtataa accagcccac
     7201 aggcgataat cccgcccagc gcgatgctga tattcgggtt aatgccctga ccgttaaagc
     7261 ccgtcgcggc aatcaccacg ccgacaaaag ccgcgctgga gccgagataa ctggggacgc
     7321 gcccgccggt aataaagaaa aacagtaacg tgccgatccc cgacattaaa atggaaagat
     7381 tgggatccag ccccatcaga atcggcatta acaccgtcgc gccaaacatc gccaccgcgt
     7441 gttgaacgcc cattactgcc gtctgagcaa acggcaatcg ttcatccggc gcgaccacgc
     7501 cgctctctgt agaggtcgat tttaactgcc agtgaggaaa accgaacatt gccatcagct
     7561 gtctccttaa ggaggttaac aagcagggcg catcagcgcg tgataactgc gatcgaacca
     7621 caccagcccg tagggtgtgg tgtgacgatg aatcgcttcg atggcgcaaa acagaatgtc
     7681 gtgggtgccg acgctcacca cctggctgat acggcagtca aacgaaacca gagcctcttc
     7741 cagttgcggg catccggtca cacccgtctg ccagcgggcg gcggcaaagc ggtgttccat
     7801 gggcgttttg ccgccaaaaa gatttgaaag cggctcctgc ccggcgctca gtgtatttac
     7861 acacagcgtt cgattttcat tgaataccgg ccagacggac gccccacgat tcaggcacac
     7921 cagtaatgtg ggcggcgtgt cggtcacgct gcagacggcg ctggcggtga acccggcgcg
     7981 cccggctgga ccgtccgtgg tgatgatatt gaccgccgcg cccatacagg acatcgcatc
     8041 gcgaaaggtt tgttgatcaa ctatgttcat agtttgctcc ttacaacagc ccgcaggctt
     8101 cttcaaagga cagacgtggc aggcgcgcaa aaagtttgct gctatcgcca tagccgatat
     8161 taatcagcag attgctcttc agcgtgctgc ccgcaaaaaa ggcgtcgtca acgtgttgac
     8221 ggtcaaaacc cgacatcggg ccggtatcca gtcccagcgc acggcaagcg acgatcagat
     8281 aagccgcctg catggaactg ttgcgaaacg ctgtttcttc ggcaagttgt gggctggagg
     8341 taaaccaact gcgggcatca ccgtggggaa acagtagtgg taaccgttca taaaattcac
     8401 tgtcccaggc gacgatagcg gtgacgggcg cggtcagggt tttttgcaga ttgccgctgg
     8461 aaagcgccgg gcgcagacgt tcttttcctt ctgcagtgcg gataaacacg atccgtgccg
     8521 gagaacagtt agctgatgtc ggcccccatt tcatcagggc ataaagctcc tgtaacgtct
     8581 catcgctgac gggtgtctcc cgccagccgt tgtgagtgcg ggcatcggtg aacagggtgc
     8641 taagcgcacc tgggctaacg gcttcgttca tagcaattcc ttacagggcg gcttcacggt
     8701 gatgtaacag gctggcaagc ccgttgagta acagagcatt aaacgtttcg ggatcggtca
     8761 cgttgcaggc gtgtccgcca tagggcatca ccattttctg gctatcgggc agggcggcat
     8821 gaagttcact ggaacatgct gttggcacca gcagatcatc actggcgcag atgatttgca
     8881 ccgggcagcg gatgcgatcc gcatggtgac taaagtcagc gcgtttgagg gcgttaagtc
     8941 gacgtagtaa attatttttg ccctgaaaat gcgccagtgc cagcgcgtct tctgcctcca
     9001 ggcgaggtgc gcgggccgcc atccagtcgg cgggatagag gaacaacggc tgcgcttcca
     9061 cccatgcttg cgcgccgccg ctatacagta atcgttcgcg aacctgaaaa cagcggcgcg
     9121 tatgggcgtt tattcgtagc cagccgttaa cgctgaccag catagttacc gacgcgggat
     9181 aatccagcgc cagctgcatc cccaccagcg caccgagcgc atggccgacc accgcgtaac
     9241 gctcaatccc tgcggctacc agcgcctgat gcagttccgc tgccatctgg gcgatactgt
     9301 aatcttctgc cagtgtgtcg ggattattgc cggtgccgcg ctggtcgtaa cagactacct
     9361 gatactcctg ctccagcacc gccagttgcg gtaaccagta actgccgcta ccgccaagac
     9421 ccgaaatcaa caccactacg ggtgcatcag cataaggggg aggtgagagt gaaagtttca
     9481 tcgcggcctc acttggcgat atgcgcaatt gtggcgattt ccaccagcgc gtcaggtttt
     9541 accagtccgc actgaatgta gaatcgcgcc ggtttatcac ccggaaaaaa ctcggcgtag
     9601 atttcgttaa tcgcggcgta atttttccag tcggtaataa agatgctgtt gaaggtcaca
     9661 tccgccatcg tgccacccgc cgtctcgatc accttgcgga tagtttccag aacgtggcgg
     9721 gtttgcgcct ttgggtcatc ggcaaacagc acgttattat gttgatcaaa agccagcgta
     9781 ccggagacat acaccacgcc atcagccagc gtgccgggaa cgaagggggc cagcggtgcg
     9841 ctgctgccag cgggaataat tacggatttt ggcatcgcta aactccttaa gcgatacgag
     9901 caaaggacgt gggagaaagc gcgtcgcaga acgtttcgac gtcgctgacc cagccaaaaa
     9961 aggtttcgat attgaacaac gcggctttct gtgcaaattc cggccccgcc tggtgtgttg
    10021 cgtcttccag caccacgccg aaatgctcca gaaaaaagcc atcgcgtagc gtcgattcga
    10081 cgcagacgtt ggtagcgata ccggtgaaaa ccagatggcg tattccacgg ctgcgtaaaa
    10141 tgctgtccag cggcgtattg aagaaaccgc tgtagcgcgg cttcggcagc acaatatcgc
    10201 caggctgcgg caccagttca tccaccagtt gataatccca ggagcctttc gccagcaatt
    10261 tcccctgcag ctgcggctgc ttacgcatgg ttttcagggc gttcgattta tgaaaattcg
    10321 gtgagccggg gccgccagcc tcgacatact gttcatccca gccattttga aaccagatga
    10381 tcaacatccc tgccgctcgc gctgcggtca cagcggtttg aatgttggca atgaccgggc
    10441 gagtggttga gacatcaaac ccggcgagat ctaagtagcc gcctggcgtg gcataagcgt
    10501 tttgcatatc caccacgatc tgcgcacttt gctgcggatc gaaggtaatg gcttccggtc
    10561 gagcggttaa ggttgtcatc atgccacctc ctgagtcagc gcagagagat gggcgcggca
    10621 ctgcatcagt ggttgaatgc gctcgccgaa ggtttcgatt cccgacagaa aatcatcgaa
    10681 ggtcaacagc acgccttctg caccaggcac gcttgcgact tcatctaaca tgcgcgcgac
    10741 actggcgtaa gaaccgacta acgtccccat attgatgttt accgccgaag tgggatcggc
    10801 catctgacga acgttggtgt cagtaccgga gcgggtatct ttctgacttt gttcggttag
    10861 ccagcttaac gcctcttcat ccgcgcccgc tttgtagtgt tcccatttgg cgcgagtggc
    10921 atcgtcggtt tcatcggcaa tcaccataaa caacacataa gagccaacgt cgcgtccggt
    10981 ttgctctgtg gcctgtttca tccgcgcagc ggtcggggcg aaagccgtgg gtgtatttac
    11041 gcctttgccg aaacagaagt tgaaatcggc atactgggcg gagaacgcca tgccagcgtc
    11101 gctttgcccg gcgcagatca ctttcatggg gatactcggt tgcggactga cgcgacaatc
    11161 attcatggtg aaaaaatcgc ctttaaaatc gcttttcccc gtgccccaca ggtcgcgcag
    11221 cacctgaaca tattcggtga gatagtcgta acgacgggag aaatagtcat cgccaggcca
    11281 gatacccatc tgctcatact cgggcttttg ccagccagtc acgaggttga cgccaaaacg
    11341 cccgccagag atggagtcga tggttgcggc catacgggcg acgattgccg gaggtaacgt
    11401 taaggtggca gcagtggcgt aaatctgaat gcgcgaggtc acggctgcca gacccgccat
    11461 caaggtgaac gactcaaggt tgtgatccca gaactcagtt ttgccgccaa agccacgcag
    11521 tttgatcatc gacagggcga aatcgaaatg gtaatgctcc gctttttgcg caatggcttt
    11581 attcagttca aaggtcggca tgtactgcgg cgcgtgggtc gaaatgagcc agccgttgtt
    11641 gccaataggt acgaatacgc caattttcat catcaacctc tcttcgtctc gtaaagtgaa
    11701 agtcagacgg ggcgctgcat cctgcatatc cttttcagcc gcgtattggc ttgtttgcaa
    11761 agcggatgcc agtttttaaa aagtgaatgt tattaatgtg ttagcattgt gttatctaaa
    11821 atatctggta aaaagtggac taaacggtca aaacagttgc acataaaaca tgcatccgtg
    11881 cgcgatgaga gtgcagaagg tcgaggccgg gcgggggttt tgctatcctg ttaccaatct
    11941 acaagagggg agagcgcatg acgcaaggcg cagtgaaaac aacgggtaaa cgttcgcgcg
    12001 cagtaagcgc gaagaaaaaa gcgattctta gcgcagcact ggacactttt tcacaattcg
    12061 gttttcacgg cacaagactg gagcagaccg cagagttggc gggtgtttca aaaaccaatc
    12121 tgctgtatta cttcccgtca aaagaggcgc tgtatattgc cgtgctgcgg cagattctcg
    12181 atatctggct ggcaccgtta aaagcgtttc gtgaagattt cgccccgctg gcggcgataa
    12241 aagagtacat ccgtctgaag ctggaagtct cacgcgatta tccgcaggct tcgcgcctgt
    12301 tctgtatgga gatgctggca ggcgcgccgt tgttaatgga tgaactgacg ggcgatttga
    12361 aggcattaat tgatgagaaa tcggcgctga ttgccggttg ggtcaaaagc ggcaaactcg
    12421 cgccgattga tccgcagcat ttgattttta tgatttgggc ttccactcaa cattacgccg
    12481 atttcgcccc tcaggtggag gcggtgacag gcgcgacgtt gcgcgatgag gtatttttca
    12541 atcaaacggt tgaaaacgtg cagcggatta ttattgaggg gattcgacca cgttaaagat
    12601 gacggaggag gttgtaacat cctccggcta cctgtttaac cgatcgtcat taaactggcg
    12661 ttaccgccag cggcagcggt attcacactc agcgaacgtt catcgatagg aattaaaacc
    12721 ccaaaaagat taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctccat
    12781 atgaacggaa ccgacgatta aactggatgg ctctgattga ttcagggtat gaatggcggt
    12841 tttttgctcc gtttccctca aaatggacgc aact
//
LOCUS       DAEEDS010000104        12417 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEEDS010000104 DAEEDS010000000
VERSION     DAEEDS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12417
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.104"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1200
                     /gene="edd"
                     /locus_tag="KLH32_003392"
     CDS             <1..1200
                     /gene="edd"
                     /locus_tag="KLH32_003392"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBE0140098.1"
                     /translation="MALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVH
                     PDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMH
                     LVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAG
                     LLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKV
                     LSGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPK
                     ANGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKV
                     RDGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQ
                     GATCITF"
     gene            1237..1878
                     /gene="kdgA"
                     /locus_tag="KLH32_003393"
                     /gene_synonym="eda"
     CDS             1237..1878
                     /gene="kdgA"
                     /locus_tag="KLH32_003393"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBE0140099.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1934..3112)
                     /gene="purT"
                     /locus_tag="KLH32_003394"
     CDS             complement(1934..3112)
                     /gene="purT"
                     /locus_tag="KLH32_003394"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBE0140100.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3246..3536
                     /gene="yebG"
                     /locus_tag="KLH32_003395"
                     /pseudo
     CDS             3246..3536
                     /gene="yebG"
                     /locus_tag="KLH32_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3603..3959
                     /locus_tag="KLH32_003396"
     CDS             3603..3959
                     /locus_tag="KLH32_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBE0140101.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4286..4945
                     /locus_tag="KLH32_003397"
     CDS             4286..4945
                     /locus_tag="KLH32_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBE0140102.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5155..7215
                     /gene="ptrB"
                     /locus_tag="KLH32_003398"
     CDS             5155..7215
                     /gene="ptrB"
                     /locus_tag="KLH32_003398"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBE0140103.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7212..7874)
                     /gene="exoX"
                     /locus_tag="KLH32_003399"
     CDS             complement(7212..7874)
                     /gene="exoX"
                     /locus_tag="KLH32_003399"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBE0140104.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7898..8554)
                     /locus_tag="KLH32_003400"
                     /pseudo
     CDS             complement(7898..8554)
                     /locus_tag="KLH32_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8656..8886)
                     /gene="holE"
                     /locus_tag="KLH32_003401"
     CDS             complement(8656..8886)
                     /gene="holE"
                     /locus_tag="KLH32_003401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBE0140105.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9025..9399
                     /gene="yobA"
                     /locus_tag="KLH32_003402"
     CDS             9025..9399
                     /gene="yobA"
                     /locus_tag="KLH32_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBE0140106.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9403..10275
                     /gene="copD"
                     /locus_tag="KLH32_003403"
                     /pseudo
     CDS             9403..10275
                     /gene="copD"
                     /locus_tag="KLH32_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10288..10629
                     /locus_tag="KLH32_003404"
     CDS             10288..10629
                     /locus_tag="KLH32_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBE0140107.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11022..12098)
                     /locus_tag="KLH32_003405"
                     /pseudo
     CDS             complement(11022..12098)
                     /locus_tag="KLH32_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12064..12345)
                     /locus_tag="KLH32_003406"
     CDS             complement(12064..12345)
                     /locus_tag="KLH32_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBE0140108.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3081 a         3032 c         3137 g         3167 t
ORIGIN      
        1 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
       61 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
      121 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
      181 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
      241 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
      301 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
      361 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc ggccgatatt
      421 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
      481 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
      541 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
      601 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
      661 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
      721 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
      781 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
      841 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
      901 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
      961 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
     1021 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
     1081 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
     1141 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
     1201 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
     1261 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
     1321 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
     1381 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
     1441 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
     1501 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
     1561 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
     1621 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
     1681 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
     1741 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
     1801 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
     1861 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
     1921 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
     1981 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagacgacgg ctgccatcaa
     2041 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
     2101 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
     2161 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
     2221 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
     2281 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
     2341 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
     2401 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
     2461 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
     2521 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
     2581 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
     2641 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
     2701 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
     2761 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
     2821 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
     2881 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
     2941 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
     3001 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
     3061 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
     3121 tcccctgatt aattccgctc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
     3181 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
     3241 tgcaaatggc ggttgaagtt taatacgtag tcattcgtga gggcgaagag aaaatgtcgt
     3301 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
     3361 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
     3421 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
     3481 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
     3541 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
     3601 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
     3661 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
     3721 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
     3781 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
     3841 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
     3901 ttcattacca ggtcagcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
     3961 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
     4021 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
     4081 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacttgatat
     4141 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
     4201 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
     4261 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
     4321 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
     4381 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
     4441 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
     4501 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
     4561 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
     4621 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
     4681 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
     4741 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
     4801 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
     4861 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
     4921 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
     4981 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
     5041 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
     5101 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
     5161 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
     5221 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
     5281 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
     5341 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
     5401 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
     5461 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
     5521 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
     5581 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
     5641 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
     5701 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
     5761 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
     5821 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
     5881 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
     5941 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
     6001 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
     6061 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
     6121 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
     6181 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
     6241 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
     6301 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
     6361 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
     6421 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
     6481 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
     6541 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
     6601 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
     6661 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
     6721 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
     6781 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
     6841 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
     6901 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
     6961 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
     7021 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
     7081 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
     7141 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
     7201 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
     7261 atgctgttca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
     7321 gaaaccgctt tgccacggta tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg
     7381 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
     7441 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccgg cggtgtctgt
     7501 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
     7561 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
     7621 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
     7681 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
     7741 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
     7801 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
     7861 ataatgcgca acatggcatg ctccaggccg aaaaagccta gcgtaatgga atgatatcgc
     7921 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
     7981 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
     8041 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
     8101 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
     8161 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
     8221 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
     8281 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
     8341 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
     8401 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
     8461 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
     8521 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
     8581 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
     8641 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
     8701 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
     8761 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
     8821 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
     8881 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
     8941 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
     9001 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
     9061 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
     9121 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
     9181 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
     9241 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
     9301 gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg
     9361 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
     9421 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
     9481 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
     9541 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
     9601 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
     9661 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
     9721 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
     9781 tttattctgc tggcgggagt ggggcatgcg acgctgaatg aaggggtaac agcgaaaatc
     9841 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
     9901 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc tattcaggcg
     9961 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    10021 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    10081 ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    10141 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    10201 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    10261 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    10321 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    10381 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    10441 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    10501 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    10561 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    10621 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    10681 caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt tattgaccat
    10741 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    10801 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    10861 gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt agttgcaaac
    10921 gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt ttttggttat
    10981 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    11041 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    11101 tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct tctgatttcg
    11161 tgaaaagttg gtgggttttc actgaacagg atacctgtta attttctggc tgcgacaaat
    11221 ttctttgtca ggccgtccgg gtgaatgctg ccgtcagggc tgttttttct aatcccggca
    11281 ctgattagat aatcccccct acttaccatg cggcactgtt caactactgc accaagtcgt
    11341 aaaccagcga caggaaggct gagtgacagg gggatagcaa tcatcattcc tgtcttaatt
    11401 tgcctgatgt ggagacgatc atcataaata tcactgaacc gcatattcgt tatgtcttcg
    11461 cgacgttgtc ctgttacaag ggctaagtcc atagctaatg ggaaccatgc cggaagttga
    11521 tctgctgcct ccctgatgca gttgtatgtc tttagtttca gccgttctct tgtaactact
    11581 attttcggtg ctcttgttgg cgttactgga ttttgagata tacgtccttc aacaatggct
    11641 tcgcggaaca tatcagataa cacagaacgc attgatcctg ccatcgtgtt tttccctcct
    11701 tcaatctaca aatcaagaaa ctcggcgata tggcgagtgg ttatttctgt cagtaaaacc
    11761 cctcctaatt tttcttttat tgtctccagt tgatttaccc gagttttata agtatttctg
    11821 gacgcttttc tccttataag aatcgttttg taacgttcaa tccagcctgc catagtaaat
    11881 gagtcgaccc ctttaagctt ttcaattagg gcagcaggag agtagttttt gtatatataa
    11941 tgatttgctt caattgcctg cgctactgca tctcttcttg aaattttacc tagtgtaaat
    12001 tcttctttcg tcagagggtt gcgccagtaa tatgctttgt ctctccttcg atatgttaag
    12061 tttctaggca aattgggatc gtattttttc cgctgcatgt tttaacttct ccattaacgg
    12121 actgtctctc ccttgtcgcc cattaggctg atggtgtgtt atatcggtat caaccttatt
    12181 tgggttgata tagaaagcct ccggaaccac cctgtaactc ctcccgtgta gttcaggtgc
    12241 aggataaatg tttccattcc ttgcccatcg tctcagcgtt gatattgatg gtgggttatc
    12301 cggatatctg agttttcccc acgttttgag tgtcacaaga ttcattgcca tacctctcac
    12361 gatatgaccg ccagtaaata tacagaatac tggcgggtgt ggttgatttt taataat
//
LOCUS       DAEEDS010000105        12398 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEEDS010000105 DAEEDS010000000
VERSION     DAEEDS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.105"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="KLH32_003407"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="KLH32_003407"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBE0140109.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..3047
                     /locus_tag="KLH32_003408"
     CDS             1647..3047
                     /locus_tag="KLH32_003408"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0140110.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3203..4738
                     /gene="gadC"
                     /locus_tag="KLH32_003409"
     CDS             3203..4738
                     /gene="gadC"
                     /locus_tag="KLH32_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBE0140111.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            4869..6188
                     /locus_tag="KLH32_003410"
     CDS             4869..6188
                     /locus_tag="KLH32_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBE0140112.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            6486..6527
                     /locus_tag="KLH32_003411"
     CDS             6486..6527
                     /locus_tag="KLH32_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140113.1"
                     /translation="MVQLVDLARCVSF"
     gene            6565..7710
                     /gene="dosC"
                     /locus_tag="KLH32_003412"
     CDS             6565..7710
                     /gene="dosC"
                     /locus_tag="KLH32_003412"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBE0140114.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            7635..8252
                     /locus_tag="KLH32_003413"
                     /pseudo
     CDS             7635..8252
                     /locus_tag="KLH32_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            8510..8842
                     /gene="ddpX"
                     /locus_tag="KLH32_003414"
                     /pseudo
     CDS             8510..8842
                     /gene="ddpX"
                     /locus_tag="KLH32_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            8856..9752
                     /locus_tag="KLH32_003415"
                     /pseudo
     CDS             8856..9752
                     /locus_tag="KLH32_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
     gene            9749..10735
                     /locus_tag="KLH32_003416"
     CDS             9749..10735
                     /locus_tag="KLH32_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0140115.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            10728..11654
                     /locus_tag="KLH32_003417"
                     /pseudo
     CDS             10728..11654
                     /locus_tag="KLH32_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11710..12141)
                     /gene="osmC"
                     /locus_tag="KLH32_003418"
     CDS             complement(11710..12141)
                     /gene="osmC"
                     /locus_tag="KLH32_003418"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBE0140116.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(12314..>12398)
                     /locus_tag="KLH32_003419"
     CDS             complement(12314..>12398)
                     /locus_tag="KLH32_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140117.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         3064 a         2987 c         3143 g         3204 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agcgatcacc
     3001 cgaaactgca gggtattgcc cagcagaaca gctttaaaca tacctgataa cgtttaacgg
     3061 taacggtgtc ccgaaacgaa cccgtttcgg gataatttcc aaagtctgtt cactggcatt
     3121 agcaacggaa aatattgttc tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg
     3181 aataccgttt tagggggata atatggctac atcagtacag acaggtaaag ctaagcagct
     3241 cacattactc ggattctttg ccataacggc atcgatggta atggctgttt atgaataccc
     3301 taccttcgca acatcgggct tttcattagt cttcttcctg ctattaggcg ggattttatg
     3361 gtttattccc gtgggacttt gtgctgcgga aatggccacc gtcgacggct gggaagaagg
     3421 tggtgtcttc gcctgggtat caaatactct gggtccgaga tggggatttg cagcgatctc
     3481 atttggctat ctgcaaatcg ccattggttt tattccgatg ctctatttcg tgttaggggc
     3541 actctcctac atcctgaaat ggccagcgct gaatgaagac cccattacca aaactattgc
     3601 agcactcatc attctttggg cgctggcatt aacgcagttt ggtggcacga aatacacggc
     3661 gcgaattgct aaagttggct tcttcgccgg tatcctgtta cctgcattta ttttgatcgc
     3721 attagcggct atttacctgc actccggtgc ccccgttgct atcgaaatgg attcgaagac
     3781 cttcttccct gacttctcta aagtgggcac actggttgta tttgttgcct tcattttgag
     3841 ttatatgggc gtagaagctt ccgcaactca cgtcaatgaa atgagcaacc ccgggcgcga
     3901 ctatccactg gctatgttac tgctgatggt ggcggcaatc tgcttaagct ctgttggcgg
     3961 tttgtctatt gcgatggtca ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca
     4021 aacctttacc gttctgatgt cccatgtggc accggaaatt gagtggacgg ttcgcgtgat
     4081 ctccgcactg ctgttgctgg gtgttctggc ggaaatcgcc tcctggattg ttggtccttc
     4141 tcgcgggatg tatgtcacag cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa
     4201 caaaaatggc gtaccggtaa cgctggtcat ttcgcagctg gtgattactt ctatcgcgtt
     4261 gatcatcctc accaataccg gtggcggtaa caacatgtcc ttcctgatcg cactggcgct
     4321 gacggtggtg atttatctgt gtgcttattt catgctgttt attggctaca ttgtgttggt
     4381 tcttaaacat cctgacttaa aacgcacatt taatatccct ggtggtaaag gggtgaaact
     4441 ggtcgtggca attgtcggtc tgctgacttc aattatggcg tttattgttt ccttcctgcc
     4501 gccggataac atccagggtg attctaccga tatgtatgtt gaattactgg ttgttagttt
     4561 cctggtggta cttgccctgc cctttattct ctatgctgtt catgatcgta aaggcaaagc
     4621 gaataccggc gtcactctgg agccaatcaa cagtcagaac gcaccaaaag gtcacttctt
     4681 cctgcacccg cgtgcacgtt caccacacta tattgtgatg aatgacaaga aacactaacc
     4741 atgtctgaat ggttataaga caagggagcg ataattcatc gctccctttt tcgtgcttgc
     4801 tgtcgtattg acttaaccgg gataagtacg agaatgagcg cacatctgtt taccggaaac
     4861 cagcacatat ggatatctgc tcccgaaaca agaaattaac gattagaaga ccagcgatac
     4921 tggttgcatt ggcactttta ctgtgtagtt gtaaaagcac gcctccagag tccatggtga
     4981 caccaccagc aggttcaaag ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg
     5041 gcatctggct ggccacggtt tctcgcctcg actggccacc ggtttcctcg gttaacatta
     5101 gtaatcccac cagccgagcc cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc
     5161 tccaacgtct cggcataaac acggtctttt tccaggtcaa gccggacggt accgccctgt
     5221 ggccatcgaa aattttgccg tggtccgatc ttatgaccgg taagattggt gaaaatccgg
     5281 gttacgatcc gctgcaattc atgctcgacg aagcccacaa gcgtgggatg aaagtacacg
     5341 cctggtttaa cccctatcgc gtatcggtta atacgaagcc cggtactatc agggaactga
     5401 atagcactct gtctcaacaa ccggcgagcg tctatgtgca acaccgtgac tggatcagaa
     5461 cgtctggcga tcgctttgtc ctcgacccgg gcatccctga ggttcaggac tggatcacat
     5521 caatagtcgc tgaagtggtt tcccgctatc cggtagatgg cgtgcagttt gacgactatt
     5581 tctatactga atcaccgggt tcacggctaa atgataacga aacgtaccgt aaatacggag
     5641 gcgcatttgc gtcaaaagca gactggcggc gcaacaatac tcagcagtta attgcaaagg
     5701 tatcacacac cattaaaagc attaagccgg gagtcgaatt tggcgttagc ccggcaggcg
     5761 tgtggcgtaa ccgatcacac gatccgctcg gttccgatac ccgaggcgcg gcagcctatg
     5821 acgaatccta cgcagacacc cgtcgatggg tggaacaagg attgctggat tacattgctc
     5881 cccaaattta ctggccgttc tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt
     5941 gggcggatgt cgttaaacca accaggaccc gcctgtatat cggtatcgcc ttctataaag
     6001 tgggtgaacc ttcaaagata gagccagact ggatgattaa cggcggcgta ccggaactga
     6061 aaaagcagct cgatcttaac gatgcggtac cagaaattag cggcaccatc ttgttccgtg
     6121 aggactatct gaataaaccg cagactcaac aagcggtcag ctatctgcaa agtcgttggg
     6181 gcagttaaac taccatgctt actttgtaac aagccggtgc tttacccacc ggcttgttac
     6241 accttgtgaa atatcccact agtaatcatg cttacataag tcaaattaac cacgcaatta
     6301 accacactta gttaaacagc tataaatccc taataaaaat gtatgaatat gaaaaaccat
     6361 acgatattcg ctgtcaccga aaaataatta cgatgggcta tactcttctg aagaacagta
     6421 gcgtagattg aaactgtctt aagtcgctta tttcttttca gtatatcttc atatttcagg
     6481 agaatatggt gcagttagtc gatctcgcac gttgcgtttc gttttgattc cgtctgattt
     6541 cccagcctta taagggtgtg aattatggag atgtatttta aaagaatgaa agatgagtgg
     6601 accggacttg tcgaacaggc agatcctctc attcgtgcta aagccgcaga aattgccctt
     6661 gcgcatgctc attatctgag tatcgagttt tatcgaattg tccgcatcga cccgcatgcc
     6721 gaagaattct tgagtaatga acaagttgag cggcagttga aaagtgcgat ggaacgctgg
     6781 attattaacg tgctttctgc ccaggttgac gatgtcgaaa ggctaataca aatccagcat
     6841 accgtcgcgg aagtgcatgc ccgcatagga attccggtag aaattgtcga gatggggttt
     6901 cgggtgctga aaaaaatcct ctatccggta atcttctctt cggattattc cgccgcagaa
     6961 aaacttcagg tctaccattt ctcgattaac agtattgata tcgcgatgga agtgatgacc
     7021 cgcgcgttta cctttagtga cagtagtgcc tcaaaggaag atgaaaacta tcgtatcttc
     7081 tcgttactgg aaaacgccga agaagaaaaa gaacggcaaa tagcctcaat actttcatgg
     7141 gaaatagata ttatctataa agtcctgctg gattctgatt taggcagcag tttgccttta
     7201 agccaggctg attttggcct gtggtttaac cataaaggtc gacattattt tagcggcatt
     7261 gccgaagtgg gccatatctc ccgtctgatt caggatttcg acggtatttt caatcaaacc
     7321 atgcgtaaca ccaggatttt gaataacaga agtttgcggg taaaatttct attacagata
     7381 agaaataccg tatcgcagat tattaccttg ctgcgtgaat tgtttgaaga agtatcgcgc
     7441 cacgaagtcg gtatggatgt actgacgaaa ttacttaacc gccgtttcct gccgactatc
     7501 ttcaaacgcg aaattgccca tgccaaccgg accggtacac cgctgtcagt gctgattatt
     7561 gacgttgata aattcaaaga gatcaacgat acgtggggcc ataacactgg cgatgaaatt
     7621 ctgcgtaaag tctcattcct ctcgcagaag agattggtga aatcgaaaat attgggcgct
     7681 gggtcatcgc ggaagcttgc cgtcagttag cagaatggcg tagccagaat attcatatcc
     7741 cggcgttatc cgtgaacttg tcggcgctgc actttcgcag taatcagctg cctaatcagg
     7801 tgtctgatgc aatgcaagcc tggggtattg acggccacca gctgacggtg gaaatcacgg
     7861 aaagcatgat gatggaacac gataccgaaa tctttaagcg cattcagatc ctgcgtgata
     7921 tgggcgtggg tttatcggta gatgattttg gcacgggctt ttccggatta tcccgcttag
     7981 tcagtcttcc ggtaacggaa atcaaaattg acaaaagctt tgtcgatcgt tgtctgacgg
     8041 aaaaacgcat cctggcctta cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa
     8101 ccgtcgtggc ggaaggggtc gaaaccaaag agcaatttga gatgctacgt aagatccact
     8161 gtcgcgttat tcagggatat ttcttttccc gcccccttcc cgccgaagaa attccaggct
     8221 ggatgagcag cgtgttaccg ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt
     8281 tttcctttaa ggtgacactg tcacttaaat actgtgattt cagctacgat tccgggagat
     8341 tccttcctta actttccctc ttgcaccaaa actgcactac gctgcacaaa tgttgatcaa
     8401 aagttaaaaa atataacttt aagccagcat tctgccgctt atcccggcat ggcatgagat
     8461 ctgcataagc ggaaagcgca gcaatttttg tcttatacag gaaccgttta tgtcggatac
     8521 caccgaactg gttgatttag ccgtgatctt ccctgatctg gagatcgaat tgaaatatgc
     8581 ctgcgttgat aacatcacag gtaaagctat ttatcagcaa gcgcgttgtc tgctacataa
     8641 agatgcgatt accgcgctgg cgaaaagtat cagtatcgcc cagctgtcag gcttacaact
     8701 ggtgatttac gatgcgtatc gcccacaaca agcacaggcg atgttgtggc aagcctgccc
     8761 agacccgcaa tatgttgttg atgtgacggt cggttctaat cacagccgtg gcacggcgct
     8821 cgacctgacg cttcgtgatg acgtggaggt ggtgaatgat gctaagcgag gaaacgtccg
     8881 ccgtacgccc acaaaaacaa acgcgattta acggtgcaaa actggtatgg atgctgaaag
     8941 gcagtccgct caccgtgacc ggcgcagtca tcattgtatt aatgcgactg atgatgattt
     9001 tttcaccgtg gctggcgacg catgatccca acgccattga tttaaccgcc cgccttttgc
     9061 cgccttctgc ggcgcactgg tttggcaccg atgaagtggg acgcgatctg tttagccgcg
     9121 tactggtcgg cagtcagcaa tcaattctcg ccggattagt ggtggtcgcc attgcgggta
     9181 tgattggttc gctactcgga tgtctatccg gtgtgcttgg cggacgcgca gacgccatca
     9241 tcatgcgcat catggacatt atgctgtcga ttccttcgtt ggtactgaca atggcactgg
     9301 ctgccgctct cgggccgagt ttatttaacg ccatgctggc gattgctatt gtgcgaattc
     9361 ccttttatgt gcgcttggcg cgggggcaag cattagttgt acgccagtat acctatgttc
     9421 aggcggcgaa aacctttggc gcgtctcgtt ggcatctgat caactgacat attttacgta
     9481 actccctacc gccgctgatc gtgcaggcat cgctggatat cggtagcgcg attttaatgg
     9541 ccgccacgtt gggatttatt ggcctaggtg ctcaacaacc gagtgctgaa tggggcgcga
     9601 tggtagcgaa tggtcgcaac tatgtactcg atcagtggtg gtattgcgca tttccggggg
     9661 cagcgatttt gcttacctcc gtcgggttta atctctttgg tgatggtatt cgcgatctgc
     9721 ttgacccgaa agcaggagga aagcagtcat gacccaaccc gttctggaca ttcaacagct
     9781 gcatttgagt ttccccggtt ttaacggtga tgttcacgcg ctcaacaatg tgtccttgca
     9841 gattaaccgc ggcgaaattg tcggtctggt gggagaatcc ggctcaggta aatcagtcac
     9901 cgcaatgctg attatgcgtc tgctaccgac gggcagttat tgcgtacatc gggggcagat
     9961 ttcactgctg ggagaagatg ttcttaacgc tcgggaaaaa cagcttcgtc agtggcgcgg
    10021 cgcacgagtg gcgatgatct ttcaggaacc gatgaccgcc cttaatccga cacgtcgaat
    10081 aggtcttcag atgatggacg taatccgcca tcatcaacca ataagtcgtc gggaagccag
    10141 agctaaagcg attgccctgc tggaagagat gcaaatcccg gatgccgtgg aagttatgtc
    10201 gcgctatccg tttgagcttt caggtggtat gcgccagcgg gtaatgattg cgctggcatt
    10261 ctcctgcgag ccgcaattga ttattgccgg cgaaccgacg acggcgctgg acgtcacagt
    10321 acagttgcag gtactgcgtt tgcttaaaca taaagcacgc gccagtggaa ctgcggtact
    10381 gttcatcagc catgatatgg ccgtggtgtc gcaactgtgc gatagcgttt acgtgatgta
    10441 tgccggaagc gtaattgaaa gcggcgggac ggcagacgtt atccatcatc cccggcatcc
    10501 gtataccatt ggtttgctgc aatgcgcacc agaacatgga gtaccacgcc agccattacc
    10561 cgccattcca gggacggtac caaacctcac ccatttgcct gacggctgcg cttttcgcga
    10621 tcgttgctat gcggcaggtg cacagtgtga aaacgtcccg gcgctgacag cgtgtggtga
    10681 caacaaccag cactgcgcct gttggtatcc gcagcaggag gtcataagtg tctgacacgt
    10741 tattaacgtt acgcgacgtc catatcaatt ttccggcccg taaaaactgg cttggtaaat
    10801 ctacggaaca tgttcatgcc attaatggta ttgatttaca gatccgccgt ggtgaaacct
    10861 tagggatcgt cggcgagtca ggctgcggca aaagcaccct cgcacagctt ttaatgggca
    10921 tgctgcaacc gagccacggg cagtacatcc gttcaggctc acaacgcatt atgcagatgg
    10981 tgtttcagga cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc atcatcacag
    11041 aatcgctctg gatagctaag cgtagtagtg aacaacagcg gcgagcgttg gcagaggagt
    11101 tggctgtgca ggtgggtatt cgtccggagt atctcgaccg cctgcctcat gcgttctccg
    11161 gcgggcagcg gcaacgcatc gccattgcca gagcactctc ttcgcagcct gacgtgattg
    11221 tgcttgatga gccaacctcg gcgctggata tctccgtgca ggcgcagatc ctcaatttgc
    11281 tggtaacgct acaggaaaat cacgggctga cttatgtgct gatttcacac aatgtctcgg
    11341 tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg
    11401 gcgacgcgca acaggtgttg actgcttcgg cacatccata cacccgatta ttgctggatt
    11461 ccctccccgc cattgataaa ccgctggagg aagaatgagc attacgtaaa acggatctgc
    11521 cgggaaaccg cacgttgccg caaggctgtt ttttctacga acgttgccct ctggcaaccc
    11581 acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgag ctccggtgct
    11641 ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc
    11701 ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga
    11761 gaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc
    11821 cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt
    11881 atccagcgac acatcggcgg tggtatcaat tgatgttggc gtgaatcccg cttcccccag
    11941 cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg
    12001 gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac
    12061 gccgctctcg gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg
    12121 acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact
    12181 acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatacggc gaaaatatta
    12241 cttctgcact taaattaaat tcccacaata gataaccggg aatatatatc tcaagagagg
    12301 taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg accttgtcat
    12361 gcagctccac cgattttgag aacgacagtg acttccgt
//
LOCUS       DAEEDS010000106        12224 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEEDS010000106 DAEEDS010000000
VERSION     DAEEDS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12224
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.106"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..493)
                     /locus_tag="KLH32_003420"
     CDS             complement(<1..493)
                     /locus_tag="KLH32_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0140118.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KLH32_003421"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KLH32_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBE0140119.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KLH32_003422"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KLH32_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBE0140120.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="KLH32_003423"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="KLH32_003423"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBE0140121.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="KLH32_003424"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="KLH32_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBE0140122.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="KLH32_003425"
     CDS             3647..4156
                     /locus_tag="KLH32_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0140123.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KLH32_003426"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KLH32_003426"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBE0140124.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="KLH32_003427"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="KLH32_003427"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBE0140125.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="KLH32_003428"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="KLH32_003428"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBE0140126.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="KLH32_003429"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="KLH32_003429"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBE0140127.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="KLH32_003430"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="KLH32_003430"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBE0140128.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="KLH32_003431"
     CDS             complement(8798..9691)
                     /locus_tag="KLH32_003431"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBE0140129.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="KLH32_003432"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="KLH32_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="KLH32_003433"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="KLH32_003433"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBE0140130.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11581..>12224
                     /gene="allE"
                     /locus_tag="KLH32_003434"
     CDS             11581..>12224
                     /gene="allE"
                     /locus_tag="KLH32_003434"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBE0140131.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNL"
BASE COUNT         2970 a         3158 c         3117 g         2979 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctgcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtaccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata acct
//
LOCUS       DAEEDS010000107        12154 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEEDS010000107 DAEEDS010000000
VERSION     DAEEDS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.107"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            127..996
                     /locus_tag="KLH32_003435"
     CDS             127..996
                     /locus_tag="KLH32_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBE0140132.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..1552)
                     /locus_tag="KLH32_003436"
     CDS             complement(1199..1552)
                     /locus_tag="KLH32_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBE0140133.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1690..3336)
                     /gene="groL"
                     /locus_tag="KLH32_003437"
     CDS             complement(1690..3336)
                     /gene="groL"
                     /locus_tag="KLH32_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBE0140134.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3380..3673)
                     /locus_tag="KLH32_003438"
     CDS             complement(3380..3673)
                     /locus_tag="KLH32_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBE0140135.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3949..5205
                     /gene="yjeH"
                     /locus_tag="KLH32_003439"
     CDS             3949..5205
                     /gene="yjeH"
                     /locus_tag="KLH32_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBE0140136.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="KLH32_003440"
     CDS             complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="KLH32_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBE0140137.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6034..7470
                     /gene="aspA"
                     /locus_tag="KLH32_003441"
     CDS             6034..7470
                     /gene="aspA"
                     /locus_tag="KLH32_003441"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBE0140138.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7588..8889
                     /gene="dcuA"
                     /locus_tag="KLH32_003442"
     CDS             7588..8889
                     /gene="dcuA"
                     /locus_tag="KLH32_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBE0140139.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9005..9343
                     /gene="cutA"
                     /locus_tag="KLH32_003443"
     CDS             9005..9343
                     /gene="cutA"
                     /locus_tag="KLH32_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBE0140140.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9319..11016
                     /gene="dsbD"
                     /locus_tag="KLH32_003444"
     CDS             9319..11016
                     /gene="dsbD"
                     /locus_tag="KLH32_003444"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBE0140141.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11053..11628
                     /locus_tag="KLH32_003445"
     CDS             11053..11628
                     /locus_tag="KLH32_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0140142.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11735..11810
                     /locus_tag="KLH32_003446"
     tRNA            11735..11810
                     /locus_tag="KLH32_003446"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11768..11770,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3102 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg
      901 aataacgccc aaccactaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1381 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1441 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1501 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1561 ccataatcaa tgagctggac gccactcgcg tcatccgtgt ctgaatctta tactagaaag
     1621 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt
     1681 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
     1741 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1801 gccagccaca gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1861 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1921 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1981 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     2041 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2101 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
     2161 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2221 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2281 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
     2341 gatagtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
     2401 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2461 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
     2521 tttcacgatg ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc
     2581 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
     2641 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2701 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2761 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2821 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2881 agcggagatg gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc
     2941 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
     3001 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3061 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
     3121 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3181 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg
     3241 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3301 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3361 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3421 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3481 cgatgtcgcc aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat
     3541 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3601 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3661 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3721 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3781 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
     3841 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3901 aaaatctacg cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc
     3961 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     4021 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg
     4081 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4141 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4201 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
     4261 caaattgccg ccggattcgg ccaagcaatg tttggctggc atagctggca actgttgttg
     4321 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt
     4381 gctaatctac aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4441 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4501 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4561 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
     4621 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4681 tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa ttgtagtaca gttgttcggt
     4741 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac
     4801 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4861 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc
     4921 tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga tcaatctgga cgctcttatt
     4981 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     5041 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5101 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg
     5161 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5221 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata
     5281 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5341 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5401 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5461 aatcagcgaa acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg
     5521 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5581 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
     5641 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5701 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgccttc
     5761 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
     5821 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5881 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
     5941 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     6001 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6061 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6121 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6181 ggtatggtaa tggttaaaaa agccgcagcc atggcaaaca aagagctgca aaccattcct
     6241 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6301 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6361 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6421 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6481 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6541 ctgcgtgaag gctttgaacg taaagctgtc gaattccagg acatcctgaa aatgggtcgt
     6601 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6661 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6721 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6781 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6841 gcgacctctg actgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
     6901 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6961 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     7021 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7081 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc
     7141 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7201 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7261 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7321 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7381 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7441 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7501 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7561 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag agctcatcat agttttgctg
     7621 gcgatcttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7681 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7741 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7801 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7861 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7921 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct
     7981 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     8041 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8101 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8161 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg
     8221 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8281 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8341 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
     8401 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8461 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8521 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8581 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8641 aaagcactga tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct
     8701 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8761 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8821 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8881 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
     8941 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     9001 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9061 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9121 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9181 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9241 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9301 agacacagat tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc
     9361 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
     9421 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9481 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9541 gccgacgagc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9601 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9661 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9721 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg
     9781 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9841 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9901 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9961 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    10021 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg
    10081 ctggcgatgt caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt
    10141 ctcacgttga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatgggg
    10201 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10261 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10321 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10381 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10441 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10501 gtcgcattct ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc
    10561 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    10621 gcatttggtg cgacgcatac cgcgcaaact caaacgcatc tcaactttac acaaatcaaa
    10681 acggtagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10741 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10801 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac
    10861 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10921 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10981 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    11041 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11101 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11161 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11221 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11281 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11341 ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11401 ctggccgatc agcaaaaaag cgctgcctac gatttcaccc acgaactgtt aaccacgctg
    11461 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11521 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa
    11581 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga
    11641 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11701 gaaatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca
    11761 ggggattgaa aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc
    11821 aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgttctgcc
    11881 tccaaatcaa ttctaccgaa ctcaaccaga ttctccccac atcaccagca atttgcgagc
    11941 atatcccaat ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt
    12001 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa
    12061 agcacaacac cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12121 gtgtcagtcc gcttgctcat gcaaagacta acaa
//
LOCUS       DAEEDS010000108        11921 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEEDS010000108 DAEEDS010000000
VERSION     DAEEDS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.108"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..2338)
                     /locus_tag="KLH32_003447"
     CDS             complement(<1..2338)
                     /locus_tag="KLH32_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBE0140143.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="KLH32_003448"
     CDS             complement(2272..2895)
                     /locus_tag="KLH32_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBE0140144.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="KLH32_003449"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="KLH32_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="KLH32_003450"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="KLH32_003450"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBE0140145.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDEKVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /locus_tag="KLH32_003451"
     CDS             7503..8225
                     /locus_tag="KLH32_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBE0140146.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="KLH32_003452"
     CDS             complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="KLH32_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBE0140147.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10297..11892)
                     /locus_tag="KLH32_003453"
     CDS             complement(10297..11892)
                     /locus_tag="KLH32_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBE0140148.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2758 a         3307 c         2944 g         2912 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccgga tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 tttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAEEDS010000109        11668 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEEDS010000109 DAEEDS010000000
VERSION     DAEEDS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.109"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            597..794
                     /locus_tag="KLH32_003454"
     CDS             597..794
                     /locus_tag="KLH32_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140149.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="KLH32_003455"
     CDS             complement(744..1937)
                     /locus_tag="KLH32_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0140150.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="KLH32_003456"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="KLH32_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBE0140151.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="KLH32_003457"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="KLH32_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBE0140152.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="KLH32_003458"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="KLH32_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBE0140153.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="KLH32_003459"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="KLH32_003459"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBE0140154.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="KLH32_003460"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="KLH32_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBE0140155.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="KLH32_003461"
     CDS             complement(10573..10716)
                     /locus_tag="KLH32_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140156.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11668
                     /locus_tag="KLH32_003462"
     CDS             10966..>11668
                     /locus_tag="KLH32_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0140157.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT         2961 a         2899 c         2993 g         2815 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atgggact
//
LOCUS       DAEEDS010000110        11601 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEEDS010000110 DAEEDS010000000
VERSION     DAEEDS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.110"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(282..357)
                     /locus_tag="KLH32_003463"
     tRNA            complement(282..357)
                     /locus_tag="KLH32_003463"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3138
                     /locus_tag="KLH32_003464"
                     /pseudo
     CDS             916..3138
                     /locus_tag="KLH32_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3474..4451
                     /gene="csiD"
                     /locus_tag="KLH32_003465"
     CDS             3474..4451
                     /gene="csiD"
                     /locus_tag="KLH32_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBE0140158.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4471..5739
                     /gene="lhgO"
                     /locus_tag="KLH32_003466"
     CDS             4471..5739
                     /gene="lhgO"
                     /locus_tag="KLH32_003466"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBE0140159.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5762..7210
                     /gene="gabD"
                     /locus_tag="KLH32_003467"
                     /pseudo
     CDS             5762..7210
                     /gene="gabD"
                     /locus_tag="KLH32_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7218..8504
                     /gene="gabT"
                     /locus_tag="KLH32_003468"
     CDS             7218..8504
                     /gene="gabT"
                     /locus_tag="KLH32_003468"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBE0140160.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8742..10142
                     /gene="gabP"
                     /locus_tag="KLH32_003469"
     CDS             8742..10142
                     /gene="gabP"
                     /locus_tag="KLH32_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBE0140161.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10163..10825
                     /gene="csiR"
                     /locus_tag="KLH32_003470"
     CDS             10163..10825
                     /gene="csiR"
                     /locus_tag="KLH32_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBE0140162.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10826..11275)
                     /gene="kbp"
                     /locus_tag="KLH32_003471"
     CDS             complement(10826..11275)
                     /gene="kbp"
                     /locus_tag="KLH32_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBE0140163.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11359..11517)
                     /locus_tag="KLH32_003472"
     CDS             complement(11359..11517)
                     /locus_tag="KLH32_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBE0140164.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2984 a         2848 c         2894 g         2875 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtagctc
     2761 tacaatgaat tccacgcatt atatatagat aaagcaggaa tatggagcgc atttgagggt
     2821 gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg attctgtgaa cattaatcac
     2881 tctcctcatg atggactagt tataataaat aaaggaaacg aagaagttga aggtacctgg
     2941 cctaacaaat tgcaacctgg aatatataaa aacatgggga gtaatagcgt taacattatt
     3001 attaataata cccgaaaaat tatcccccct tgtaaagtat ttacgcttag aggcggatct
     3061 ctaaatatca atattcctag gcgtagcgct cttcttttag ggaaaacagg agaaccgccg
     3121 aactatctct atttataatt taatgttata tctgccccga taaaacgggg cagataatat
     3181 gtttagttta ctaacggtca ttttgtagtg aagccattta ctgtttttta tcgaccagat
     3241 aatctgttct ctaatgttaa ctccccctaa cctgttgctt tagttattca tttcctgtct
     3301 cactttgcct taatacccta cgttaaatgt tactaatttg ttgcttttga tcacaataag
     3361 aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt agatattttt agattatggc
     3421 tacgaaatga gcatcgccat gtcaccctac atctcataag aggatcgctt ctgatgaatg
     3481 cactgaccgc cgtacaaaat aacgctgtcg attcaggcca ggactatagc ggattcactc
     3541 tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac cttcaccgaa cagacgacca
     3601 aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc gctggagtac aaatcgtttc
     3661 tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc gaatcagctg caaccattgc
     3721 tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt gatcaatgcg gtaggtatcg
     3781 atgatgtggc gcaggcagat gagatggtga agctggcaac ggcggtagcg catctgattg
     3841 gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc gcgtttcgtg gtgaaaaatg
     3901 tcgataactc agacagctat ctgcgtcagc cgcaccgcgt gatggaactg cacaacgacg
     3961 gcacgtatgt cgaagagatc accgattacg tgctgatgat gaaaatcgat gagcaaaaca
     4021 tgcagggtgg aaattcgttg ctgctgcatc tcgatgactg ggaacatctg gaccactatt
     4081 tccgccatcc actggcgcgt cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca
     4141 gcaaagatgt tttccatcca gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct
     4201 atatcgacca gttcgtccag ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt
     4261 ccgatgccat tgaaaccagc aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc
     4321 tgttgataaa caacctgttc tggctgcacg gtcgcgaccg ctttactccg cacccggatc
     4381 tgcgccgtga actgatgcgt cagcgtggct atttcgctta cgccactcac cactaccaga
     4441 cgcatcagta agcgcaaagg aattgagcgg atgtatgatt ttgtgattat tggcggcggc
     4501 atcatcggca tgtcgaccgc catgcaactg attgatgttt atccggatgc ccgcattgcg
     4561 ttgctggaaa aagagtccgg cccggcctgt caccagacgg gccacaacag cggcgtgatc
     4621 catgccgggg tctattacac gcccggcagc ctgaaggcgc agttttgcct ggcggggaac
     4681 cgtgccacca aagccttttg cgatcaaaac ggcattcgct atgacaactg cggcaagatg
     4741 ctggtcgcca cgtctcagct cgaaatggaa cggatgcgcg ccttatggga acgcacagcg
     4801 gcgaacggta tcgagcgcga gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat
     4861 atcaccgggc ttggtggcat ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg
     4921 acggcggcaa tggcaaaaat cttccaggcc agaggcggcg agattattta taacgccgaa
     4981 gtcagcgccc tcagtgagca taaaaacggc gtggtgatac gcacccgtca gggcggtgaa
     5041 tatgaagcat cgacgctgat tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg
     5101 ctcggcctcg aaccgggctt tattatctgc ccgttccgtg gcgagtattt ccgccttgcg
     5161 ccggagcata accagattgt taaccacctg atttacccca ttcccgaccc cgcgatgcca
     5221 tttttgggcg tgcatctcac ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg
     5281 gtgctggctt tcaaacgcga aggctatcgc aagcgcgact tctcatttag tgacacgctg
     5341 gagattttga gctcgtcggg gattcgccgg gtgctgcaaa accatctacg ctcaggactg
     5401 ggcgagatga aaaactcgct gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt
     5461 ccccggcttt cgttaagcga tctccagccc tggcccgccg gtgtgcgggc gcaggcagta
     5521 tcgccggacg gcaagctgat tgacgatttt ctgtttgtca ccaccccacg cacgatccac
     5581 acctgcaatg cgccctcccc ggcagcgaca tcagcaattc ctattggtgc gcatattgtc
     5641 agcaaggtac aaacgctgtt ggcaagccag agtaaccccg gacgcacgct gcgagcggca
     5701 cgtagtgtgg atgccttaca cgccgcattt aatcaataac ctttgaaaac aggacgtaac
     5761 aatgaaactt aacgacagta acttattccg ccagcaggcg ttgattaacg gggaatggct
     5821 ggacgccaac aatggtgaag tcatcgacgt caccaatccg gcgaacggcg acaagctggg
     5881 tagcgtgccg aaaatgggcg cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc
     5941 cctgcccgcc tggcgtgcgc tcaccgccaa agaacgcgcc accattctgc gcaactggtt
     6001 caatttgatg atgcagcatc aggacgattt agcgcgcctg atgaccctcg aacagggtaa
     6061 accgctggct gaagcgaaag gcgaaatcag ctacgccgcc tccttcattg agtggtttgc
     6121 tgaagaaggc aaacgtattt atggctacac cattcctggt catcaggccg ataagcgcct
     6181 gattgttatc aagcagccga ttggcgtcac cgccgccatc acgccgtgga actttccggc
     6241 ggcgatgatt acccgtaaag ccggtccggc gctggcggca ggctgcacca tggtgctgaa
     6301 gcccgccagt cagacgccgt tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg
     6361 cattccggct ggcgtgttta acgtggtcac cggttcggca ggcgcggtcg gtaatgaact
     6421 gaccagcaac ccgctggtac gcaaactgtc gtttaccggt tagaccgaga ttggccgcca
     6481 gttaatggaa cagtgcgcga aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc
     6541 gccgtttatc gtctttgacg atgccgatct cgataaagcc gtggaaggcg cgctggcctc
     6601 gaaattccgc aacgccgggc aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg
     6661 cgtgtatgac cgctttgccg aaaaattaca gcaggcggtg agcaaactgc acatcggcga
     6721 cgggctggag aaaggcgtca ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt
     6781 ggaagagcat attgccgatg cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc
     6841 gcacgaacgc ggcggcaact tcttccagcc aaccattctg gtggacgttc cggccaacgc
     6901 caaagtgtcg aaagaagaga cgttcggccc cctcgcccca ctgttccgtt ttaaagatga
     6961 agccgatgtg attgcacaag ccaatgacac cgagtttggt cttgccgcct atttctacgc
     7021 ccgtgattta agccgcgtct tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat
     7081 caataccggc attatttcca atgaagtggc cccattcggc ggcatcaaag cctcgggtct
     7141 gggtcgtgaa ggttcgaagt atggcatcga agattactta gaaatcaaat atatgtgcat
     7201 cggtctttaa ctggagaatg cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc
     7261 aggcgattcc ccgtggcgtt gggcaaattc acccgatttt cgctgaccgc gcggaaaact
     7321 gccgggtgtg ggacgttgaa ggccgtgagt atcttgattt cgcgggcggg attgcggtgc
     7381 tcaataccgg gcacctgcat ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac
     7441 tgtcgcacac ctgcttccag gtgctggctt acgagccgta tctggagctg tgcgagatta
     7501 tgaatcagaa ggtgccgggc gatttcgcca agaaaacgct gctggttacg accggttccg
     7561 aagcggtgga aaacgcggta aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg
     7621 cttttagcgg cgagtatcac gggcgcacgc attacaccct ggcgctgacc ggcaaggtga
     7681 atccgtactc tgcgggcatg gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt
     7741 gcccgctgca cggcataagc gaggatgacg ctatcgccag catccaccgg atcttcaaaa
     7801 atgatgccgc gccggaagat atcgccgcca tcatgattga gccggttcag ggcgaaggcg
     7861 gtttctacgc cgcgtcgcca gcctttatgc agcgtttacg cgctctgtgt gacgagcacg
     7921 ggatcatgct gattgccgat gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg
     7981 cgatggagca gatgggcgtg gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg
     8041 gtttcccgct ggcgggcgtc accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg
     8101 gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga
     8161 aggtgttcga gcaggaaaat ctgctgcaaa aagccaacga tctggggcag aagctaaaag
     8221 acggattgtt ggcgatagcc gaaaaacacc cggagatcgg cgacgtacgc gggctgggat
     8281 cgatgattgc catcgagctg tttgaagatg gcgatcacag caaaccggac gcaaaactca
     8341 ccgccgagat cgtggcacgc gcccgcgata agggcctgat tcttctctcc tgcggcccgt
     8401 attacaacgt gctgcgcatc cttgtaccac tcaccattga agacgctcag atccgtcagg
     8461 gtctggagat tatcggtcag tgttttgatg aggcgaagca gtagcaccgc ccccatgcag
     8521 gaggcgacgc tgcgcgtctt gtccggccta cgagaatcca agtcggataa ggcgtttacg
     8581 ccgcatccga caatctgtac gtgaacagga ataaatctat gtcggccgga taaggcggag
     8641 ccgctctccg gcaaaaagaa tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc
     8701 ggggcgctct cccaagtgac acactttcga gaggattcag gatggggcaa tcatcgcaac
     8761 cacacgagtt aggcggcggg ctgaaatcac gccacgtcac catgttgtct attgccggtg
     8821 ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg
     8881 tattactggc ctatctgttc gccggattac tggtggttat gattatgcgg atgctggcgg
     8941 aaatggcagt tgctacgccc gataccggtt cgttttccac ctatgccgat aaagccattg
     9001 gccgctgggc gggctatacc atcggctggt tgtactggtg gttttgggta ctggttatcc
     9061 cactggaagc caacatcgcc gccatgatcc tgcactcgtg ggttccaggc attcccatct
     9121 ggttattttc cctcgtcatt accctcgcct taactggcag taatttatta agcgttaaaa
     9181 actacggcga atttgagttc tggctggcgc tgtgcaaagt catcgctatc ctggccttta
     9241 ttttccttgg tgcagtcgca attagcggtt tttaccctta tgccgaagtg agcgggatct
     9301 caagattgtg ggatagcggc ggctttatgc ccaacggttt cggtgcggta ttaagcgcga
     9361 tgttgatcac catgttctcg tttatgggcg cagaaattgt caccattgcc gccgcggaat
     9421 ccgacatgcc ggaaaaacat attgtccgcg ccaccaactc ggttatctgg cgtatttcta
     9481 tcttctattt gtgctctatt tttgtcgtcg tggcattaat accgtggaat atgccggggc
     9541 tgaaagccgt tggatcttat cgctcggttc tggaattgct caatattccc catgcgaaat
     9601 taatcatgga ctgtgtgata ttactttccg taaccagttg cctgaactcg gccctgtata
     9661 ccgcctcaag gatgctctac tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta
     9721 aaatcaaccg cagtaaaacc ccgtacgtgg cggtgttact ctccaccggc gcagcattct
     9781 taacggtggt ggtgaactat tacgcgcctg cgaaggtatt taaatttctg atcgacagct
     9841 ccggcgctat cgccctgctg gtttatttag tcatcgccgt ttcacagttg cggatgcgca
     9901 aaattctgcg agcagaagga agcgaaattc gcttgcgaat gtggcttgat ccctggctca
     9961 cctggctggt catcagcttt attacctttg tgttggtagt gatgctattc cgtccggcgc
    10021 aacagttaga agtgatctcc accggcttat tagcgatagg gattatctgt accgtgccaa
    10081 ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac acccgttcat aatacgcgct
    10141 gataatgatc aggagtcaca ccatgaccat tacgtctctg gatggctatc gctggctgaa
    10201 gaacgatatt attcgcggta attttcaacc ggatgaaaaa ttacggatga gtttactgac
    10261 atcgcgttat gcacttggcg ttgggccgtt gcaggaagca ctttctcaac tggtggcgga
    10321 acggctggtc acggtggtga atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga
    10381 gctgctcgat attttcgacg cccgcgccaa tatggaagcg atgctggtca gtctggcgat
    10441 tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa
    10501 gctggaagcc tgtgacgcca gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc
    10561 gtttcataca gcaattgtgg cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg
    10621 gttgtttgat ctggcggcgc gttatcgatt tatctggctg cggcgaacgg tgctttcggt
    10681 ggaaatgctg gaggataaac acgatcagca ccagaccctg actgcggcgg tactgccgcg
    10741 agatacagcg cgcgccagtg agttaatgcg ccagcattta ctgacgccaa ttcccattat
    10801 ccagcaggcg atggctggca attaactact cttccggaat acgcaacact tgccccggat
    10861 aaattttatc cgggcttttt agcatcggtt tattcgcttc gaagatttta ttgtacagat
    10921 tagcgttacc gtagacctgt ttggaaatgg cactcagagt gtcgccagac ttaacggtat
    10981 aaaactggct ggcagtggct ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac
    11041 cggaaatatt ccccaccgca acaaggattt tctccttcgc ctcctgactc aggccgtcac
    11101 cagtgaccgt cgctttgccg tcggcaattt gaatgttcac tttatcggca tcaggtatac
    11161 cggttttgct cagatgctcc tgcaccttct tcgcctgatc gtctttatcg tgctgacctg
    11221 taaccgcatc ccagagtttt tctccggcat ctttaacaaa attgaacaga cccatagcta
    11281 cctcattcgt taacggaaac actcagaaag tgtagcagag gaacgcggcg cgttaacgcc
    11341 gcgggggtga gcacaggttt aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg
    11401 gatataccca agcagtgtca gcagaatatt aataatgaac gcccaaccga accctttacc
    11461 gagcagcacg ccgatcggcg gcagaatgat ggtgatgacg attctccaga aacccatatg
    11521 tactccctat aagaaaatta ctcattgttt aaaaagagat tttatctctt aagcgtagta
    11581 attttagcgg agcctgccag t
//
LOCUS       DAEEDS010000111        11547 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEEDS010000111 DAEEDS010000000
VERSION     DAEEDS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.111"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..60)
                     /locus_tag="KLH32_003473"
     CDS             complement(<1..60)
                     /locus_tag="KLH32_003473"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140165.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            171..1661
                     /locus_tag="KLH32_003474"
     CDS             171..1661
                     /locus_tag="KLH32_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBE0140166.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="KLH32_003475"
     CDS             1713..2870
                     /locus_tag="KLH32_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE0140167.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2836..2958)
                     /locus_tag="KLH32_003476"
     CDS             complement(2836..2958)
                     /locus_tag="KLH32_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140168.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3161..4846
                     /locus_tag="KLH32_003477"
                     /pseudo
     CDS             3161..4846
                     /locus_tag="KLH32_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4884..7244
                     /locus_tag="KLH32_003478"
     CDS             4884..7244
                     /locus_tag="KLH32_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE0140169.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7301..10084
                     /locus_tag="KLH32_003479"
     CDS             7301..10084
                     /locus_tag="KLH32_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE0140170.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10445..>11547
                     /locus_tag="KLH32_003480"
     CDS             10445..>11547
                     /locus_tag="KLH32_003480"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0140171.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT         3353 a         2595 c         2555 g         3044 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     3061 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3121 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3181 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3241 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3301 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3361 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3421 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3481 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3541 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3601 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3661 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3721 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3781 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3841 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3901 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3961 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     4021 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     4081 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4141 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4201 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4261 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4321 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4381 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4441 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4501 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4561 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4621 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4681 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4741 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4801 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4861 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4921 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4981 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     5041 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     5101 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5161 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5221 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5281 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5341 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5401 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5461 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5521 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5581 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5641 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5701 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5761 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5821 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5881 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5941 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     6001 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     6061 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6121 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6181 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6241 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6301 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6361 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6421 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6481 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6541 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6601 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6661 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6721 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6781 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6841 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6901 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6961 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     7021 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     7081 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7141 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7201 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7261 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7321 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7381 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7441 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7501 agaagacaat gagctcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7561 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7621 cgatgttaat gcctatatca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7681 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7741 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7801 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7861 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7921 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7981 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     8041 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     8101 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8161 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8221 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8281 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8341 tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat
     8401 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8461 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct
     8521 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8581 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8641 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8701 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8761 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8821 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8881 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8941 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     9001 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     9061 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9121 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9181 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9241 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9301 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9361 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9421 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9481 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9541 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9601 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9661 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9721 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9781 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9841 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9901 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9961 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
    10021 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
    10081 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10141 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10201 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10261 acacctttcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10321 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10381 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10441 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10501 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10561 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10621 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10681 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10741 ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt
    10801 tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg
    10861 gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg
    10921 cggtccggtg caaatctgct ggcataaatt cgcccgctac tgggatgtgg agctgcgtga
    10981 gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg aagcctgcga
    11041 cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta actatgagtt
    11101 cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta tcgacatcga
    11161 catgcacatc gacgccgcca gcggtggctt tctggcaccg ttcgtcgccc cggatatcgt
    11221 ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata aattcggtct
    11281 ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc cgcaggaact
    11341 ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca acttctcccg
    11401 cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc gtgaaggcta
    11461 taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg agatcgccaa
    11521 actggggccg tatgagttca tctgtac
//
LOCUS       DAEEDS010000112        11304 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEEDS010000112 DAEEDS010000000
VERSION     DAEEDS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.112"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KLH32_003481"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KLH32_003481"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBE0140172.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KLH32_003482"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KLH32_003482"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBE0140173.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KLH32_003483"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KLH32_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBE0140174.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="KLH32_003484"
     CDS             6038..6658
                     /locus_tag="KLH32_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBE0140175.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="KLH32_003485"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="KLH32_003485"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBE0140176.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KLH32_003486"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KLH32_003486"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE0140177.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KLH32_003487"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KLH32_003487"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBE0140178.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="KLH32_003488"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="KLH32_003488"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBE0140179.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KLH32_003489"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KLH32_003489"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2654 a         3251 c         2781 g         2618 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccactg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccattgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaagg
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggc
//
LOCUS       DAEEDS010000113        11087 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEEDS010000113 DAEEDS010000000
VERSION     DAEEDS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.113"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            154..504
                     /locus_tag="KLH32_003490"
     CDS             154..504
                     /locus_tag="KLH32_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBE0140180.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="KLH32_003491"
     CDS             557..952
                     /locus_tag="KLH32_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBE0140181.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="KLH32_003492"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="KLH32_003492"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBE0140182.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="KLH32_003493"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="KLH32_003493"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBE0140183.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KLH32_003494"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KLH32_003494"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBE0140184.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="KLH32_003495"
     CDS             complement(5323..6423)
                     /locus_tag="KLH32_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBE0140185.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KLH32_003496"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KLH32_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBE0140186.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="KLH32_003497"
     CDS             complement(7571..8137)
                     /locus_tag="KLH32_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0140187.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="KLH32_003498"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="KLH32_003498"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBE0140188.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="KLH32_003499"
     CDS             10263..10751
                     /locus_tag="KLH32_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBE0140189.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10869..10937)
                     /locus_tag="KLH32_003500"
                     /pseudo
     CDS             complement(10869..10937)
                     /locus_tag="KLH32_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2702 a         2833 c         2643 g         2909 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 tatagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagtatgg
    11041 cccagccttt ccagcaatcg tcgattgtta taccagtcca cccacgt
//
LOCUS       DAEEDS010000114        11084 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEEDS010000114 DAEEDS010000000
VERSION     DAEEDS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.114"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            404..562
                     /locus_tag="KLH32_003501"
     CDS             404..562
                     /locus_tag="KLH32_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBE0140190.1"
                     /translation="METLSTNLQLARLVGVQGTPATIIGDEMIPGAVSWETLEAVVKE
                     KLAVAHAQ"
     gene            552..1058
                     /locus_tag="KLH32_003502"
     CDS             552..1058
                     /locus_tag="KLH32_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HBE0140191.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            1241..2056
                     /locus_tag="KLH32_003503"
     CDS             1241..2056
                     /locus_tag="KLH32_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HBE0140192.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            2403..4289
                     /locus_tag="KLH32_003504"
     CDS             2403..4289
                     /locus_tag="KLH32_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HBE0140193.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            4330..4857
                     /locus_tag="KLH32_003505"
     CDS             4330..4857
                     /locus_tag="KLH32_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HBE0140194.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            4961..6340
                     /locus_tag="KLH32_003506"
     CDS             4961..6340
                     /locus_tag="KLH32_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HBE0140195.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            6343..7626
                     /locus_tag="KLH32_003507"
     CDS             6343..7626
                     /locus_tag="KLH32_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0140196.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            7616..8746
                     /locus_tag="KLH32_003508"
     CDS             7616..8746
                     /locus_tag="KLH32_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0140197.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALSAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKMVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            8751..9446
                     /locus_tag="KLH32_003509"
     CDS             8751..9446
                     /locus_tag="KLH32_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0140198.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            9433..9918
                     /locus_tag="KLH32_003510"
     CDS             9433..9918
                     /locus_tag="KLH32_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBE0140199.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAVFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPPELEATFARLAA
                     R"
     gene            9943..10428
                     /locus_tag="KLH32_003511"
     CDS             9943..10428
                     /locus_tag="KLH32_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140200.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            10550..>11084
                     /locus_tag="KLH32_003512"
     CDS             10550..>11084
                     /locus_tag="KLH32_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HBE0140201.1"
                     /translation="MKKLILAAAVAGAVLLSSAAQAQTTPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNQWSEWDVPGGQLTTKGGVLEIYMGHYMREWLAELGMVTSGECP
                     TPDTVYTYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SQKAVQAMEKERSQMQLD"
BASE COUNT         2455 a         2738 c         3335 g         2556 t
ORIGIN      
        1 tcggggcaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat cttgttgtct
       61 gattattgat ttttcgcgaa accatttgat catatgacaa gatgtgtatc caccttaact
      121 taatgatttt taccaaaatc attaggggat tcatcagggt gatgcgtgca ctacgcaaag
      181 gccaggcctc agcattttat tatggtgatc ccctgggcga aatgcgcctg gtaagcagag
      241 tttttgaaat gtaaggcctt tgaataagac aaaaggctgc ctcatcgcta actttgcaac
      301 agtgccgtgc agacagaaac cgtaaacacc ggcattgaca tgccggggaa gcccttatag
      361 tacaataatt ctctatatcc aaactgaccc cgatgagaaa agcatggaga cgttgagcac
      421 caatctgcaa ctcgcccggc tggttggcgt tcaggggacg ccggcaacca tcattggcga
      481 cgagatgatc cccggcgcgg tatcgtggga gacccttgag gcggtggtga aagaaaaact
      541 ggcggtggcc catgctcagt aaactgcgcc gctggctgcg tgaaggcgcg atcttactgg
      601 ttgtgctggc tggcgtgata atcctgctgg atgtctggcg ttctccgcag atgccggcca
      661 tgtttgacag cacgccgctg catacgctgg acggggaaac cgtaacgctg gcgtcgatca
      721 gtgaagaacg cccggtgctg ctctacttct gggcgagctg gtgcggtatc tgccgtttca
      781 ccacgccgga tgtcgcccgt ttgcagtcgg aaggggagag cgtcatgacc atcgcgctgc
      841 gttccggtaa tgacggggag gtttcccgct ggttatcgcg taaaagggtg acgttccccg
      901 tcgtgaatga tagcggcggt gagatatcac gaaactggga aatcagcgtc acgccaacgc
      961 tggtggtggt gtcgaagggg caggtggtaa ctacgaccag cggctggacc agctactggg
     1021 gcatgaagtt acgtctgtgg cgggcagcca tgttttgata gcgtgccggg gaaacccggc
     1081 attttttaac ccattgtaag gatgtgtaaa acgagcggag agcgtgtttt ctgttatttt
     1141 tgctgctttt ttaccgacgc cagttattat tttttaccgc tgcctgacgt tgcaaacctg
     1201 aattgataat ggcatatctg ttttaattga atgaaagtct gtgatacgtt caggcgattt
     1261 tttcgatttc cattttgaca gggataacaa ttcaatgcgc aagggtaaaa ttattgttgc
     1321 atcaatgatg gtgctttgtg tccttatttt tgttgcggcg gcggcatggt ttatgctgtt
     1381 ccagggagaa atgacggagc cgacagagga ggaaaaacag gcggcaattt tacacgctgc
     1441 gggcctgatg aaatcagaga cccaggataa aaaatcagtc gaaacgctct atcatcgtta
     1501 tattattcaa cgccatgtta atttagattc cggcgaactg gtggctggca gcagcgcgga
     1561 caccgcgcgg cagaagtgtg aaaaacttgc ccctgaacgc gacccagcgc aggttcgtca
     1621 gcgctgtacg gtggcggatg tgttctttgt gaaagataaa aataatgaaa ttcagcaggt
     1681 tatcattcca gttaccggta agggtgcgaa atccatgatg catgcgtttc ttgctctggg
     1741 gctggacggt cgtacggtga gaaatctgta ttattatcag caacgggaaa cgccgtttct
     1801 gggggctagg gtggaagacg ccaactggcg caaacagtgg ccagggaaac gattattaga
     1861 taacagcgga catcctgcat tgaaaatagt acaggataaa cctgaacatg ccgatgaata
     1921 taccgttgac ggtatttccg gtgcgacgtt gacatcaacc ggcgttgaga aaagtattaa
     1981 ctactggatg gggccgcagg gatatggtca attcttacag cgtcttgcca gcgatcgaaa
     2041 taaccttaat ctttgaatat tgctgtacgc aataacgcgt tttatttctg cctgccgata
     2101 atgaataata tcactgtcgg cagggggatt tattcctctg ttaccgcgcc tgccttgccg
     2161 ttaattccat gagaaaaaca gggagataac gtccctttta tcaacgcata atgtgtgtgt
     2221 tcatcacgat ttgatgaaat aagtttacat aacttgtcgt ctgctgacat ttttatgatc
     2281 caggtcatgt ataacgcgac aaaaagatat gataataatt atcatttgta ttctttggcg
     2341 gtgtgcacgt gcgtaacttc cgtaactctt tgttggttac tctctttggc ttgtttataa
     2401 gcatgagtgt ctgggcgtcg acgaattatg cgcctttgat tgaagatatc gagcaacgtc
     2461 ttgataaaac agcggaactt tatcagcaac agcatgccga cgaggcgcgc cgcacggtac
     2521 aaatggccta ttttgaggtg ttcgagaacc tggaagggcc gatccgcatc aacatttccg
     2581 cgcgcaaaag ttacgagatg gagagcgcat tcggcgagat ccgtcgaatg attggcgaga
     2641 aaaaaccgct cgctgacgtc caggcgcgca ttgactggct gaaagccgca ctgcgcgaag
     2701 tggaaccggt gctggatggc ggacatcgtc tggtggccga agagcagcac aacgcgctgt
     2761 ccagggacga cattgccgtt cactggcagg agagtttcag gacgatcgac gatctgctgg
     2821 cgcaggcggt cactgaatat caggccggga attacagcgt tgccagtcag cacgttcaac
     2881 aggcgcacta tcagggcttt aaaaactctg agatggagat gtccctcaga caaaaccgtt
     2941 ctgcaaaaga tgccgcctcc attaaccaac aattctcatc cctgattgcg ctgaccgcgc
     3001 aacctgacca tctcaacgat gtctcctatc aggtcactac gctgctacag gatatcgagg
     3061 atatcttacc cggcttgcca accacccgtg acgatcagca ggttgccgca gcgcaaagcg
     3121 cagacaacat tccggcggcg gacgagggaa ataaccgcac cgactgggct gaggtgacga
     3181 cgggaattaa ccagagcatt caggacgcga tagcgcgcta tcagggcggc gatgcgaaga
     3241 atgcgattct ggatatccag gatacctact tcgaccgctt cgaagccagc ggcatggaaa
     3301 acaaaatcgg ctctcgcgat tcggcattta aaaccacgct ggaggcgtat tttacccgcc
     3361 tggtcagtct gatgaaggcc ggtcagccgg ttgagcgcct gaatgcagag gccagcgcgc
     3421 tgacgcagga tctgcaaaaa gccgtgacga tgctgggcga aggcgaagaa acccagtgga
     3481 gtctgctgct ctatagtctg atgatcatcg tgcgcgaggg gctggaagcg ctgctgattg
     3541 ttgcggcaat cgtggcctac atggtgaaaa acaaccatca ggataagctg ccgcttattc
     3601 gtcagtcggt aattgtggcg ctgatcgcca gcgtcatcac cgccgctatc ttccagatgc
     3661 tgtttactaa ctccggcgcc agccgtgaac tgctggaagg cataacgatg ctgattgccg
     3721 tcgtgatgct gttctttatg agttactggc tgctgtccaa agtggaagcg cgccactgga
     3781 aagcctggct ggaaggcaaa ctgtctcatt ccctgtcgcg aggctccctg gtcggactat
     3841 ggctgaccag cttcctggcg gtgtaccgcg aaggggcgga aacggtgctg ttctattacg
     3901 ctctgattgg cgatgcaaac acggtatccg gccatatggc gattggcgcc ggttttgtga
     3961 ttggctgcgt ggtgctgctg ctcgcctggc tggtgatgcg ttactcagtg gtgcgcctgc
     4021 cgctgaaacc cttctttatg tttaccggca gtttcatgta tctgatggcc ttcgtctttg
     4081 cgggcaaagg cgttctggaa ctggtggaag gcaaactgtt ccagcctacg ttgattaacg
     4141 gcttccctga aatcagctgg ctggggattt atccctacgt ggaaacgttg ttaccgcagg
     4201 tcgtattgct gctcgcggcg ctggtggcgc tgtgggtgat gcggcgcaaa agcgcggttc
     4261 ccggggagac gataaaaaat aacccataat cgttgtatag ccgttgtttt agacgagagg
     4321 atacgtttga tgaccatgaa gaaaaccctg attgccagcg cagtgatggc cagcattttc
     4381 attgcgccag ccgcgtttgc ttttaaagag tacccggcag gcgagcctgt caccatgaat
     4441 gaaatggagc tggcggccgt ttacctgcaa ccgattgata tggaaccgcg cggcatgggc
     4501 ttaccggcag caaaagccga tgttcacctt gaagcggata tccacgctgt agaaggcaat
     4561 aaaaacggct ttggcgcagg ggaatggatc ccgtacctga ccatcagcta caccctggtc
     4621 aacaacgaca cgggcgaaaa acaggaaggc accttcatgc cgatggttgc cagcgatggc
     4681 ccgcactatg gcgcgaacat caaaatgatg ggcgtgggca actataaagt gacctaccac
     4741 attgaaccgc cgtcaaaagc cgggatgcat cgccatactg acagcgaaac aggcgtaggc
     4801 cgctggtgga aaccgtttga tgtgagctac gagtttaaat atgtcggtct gaactaaaac
     4861 cgactaacca acgcgccagg aggattcccc cttctggcgc gttggtcaga ccgatcatat
     4921 gcccgtcgct taattcaacc cgccgtacag agtcgaagtt atgagttact ttttcgtcac
     4981 gacgctacag gtctttttct gcattgcgct tctttccggt gttctctgga gtcgaaacga
     5041 tcctccctct ttacgtccgc tgacctggac gctgctgacc gggctgattg ccggcgtgct
     5101 ggcgggactg tttattcacg gcagccaacc ggtacaactg ctgctggttg gggcggaggt
     5161 catggtatcg ctgctgtttg tcctcagttt ctggtgggct tcaacgcgta tccgctatct
     5221 gtggcagggg attctgattt tcggcgctgc ccgccattgg gcgctggacc ctaatctggg
     5281 cgggctgacc agtacgcatg tgctgaatac tgacctgctg ttaaacctga cggccgtcgt
     5341 tctcgctttt gcgatcctct gtctggcggg cgtactctgc gcgatgctgc tgcggcgtat
     5401 tcgcggcctg tactggccgt taacgctgat tctgctggtg atgatctggc tgccgctgag
     5461 cggcaacctg ctgttgttgc tgatgaaatt acaggttgtt ccgctgggaa aatcactgct
     5521 gagcttcgtg gcgaaagtca caaataacac ggcgctgtat aactgggcgg gagccgcgct
     5581 gctgctggcc ctggcgctgt gctggcttcc ggcgctgttg cgggcttttc gacagacgcg
     5641 ggaaaccgaa gaaccgattg ctcaccgtct ggcgctggcg cagcgtcgca acgctttgcg
     5701 gctgtggttg gtcacaatcg gctgcgcggt ggtcgttatc gcaggccaac tgtggtggga
     5761 taaagtggcc tcgcagccgc cgcaactctc tgaagccgtg cccgtaacgc tggggagcga
     5821 tggcatggtg cgcctgccgg ttgaacagct tcgtgacggt aaactgcatc gtttcgtctg
     5881 ggtggcggac gatggcaaag cggtgcgctt cttcgtgatt aaccgctatc ccgacaagct
     5941 gcgttttggc gtggtgttcg acgcctgcct gctgtgcggc gatcagggtt atgtcatgga
     6001 gggcaatcag gttatctgcg tcgcctgcgg agtgcatatt tttattccgt ctatcggcaa
     6061 ggccggtggc tgtaacccgg tgccgattga aaactggcac aatgatgaaa aagagctggt
     6121 gatccccggt aaagaactgg cgacaggggt gaactacttc tcgacggtca tgactattaa
     6181 agtgaccgat ccggtcgatg gttcaacgtt gaccaatacc tcagccgatt acaaatacag
     6241 ctatggcggc aagacctggt tcttctcgtc agaggctaac tatgaacgct tccgtgaaac
     6301 gccggagcag tttgttcctg ccgacatgag ggaggaataa tcatgctgtg gcggatgtta
     6361 cgacaatcct gggggcgcaa tttacgccgc aaagtgctgg cgataatcac cgtttttctg
     6421 gcctccagcc tgatctcggc gctgctggcg gtgtccattg atatcggcga caaaatgtcc
     6481 agagagctga agtcttatgg tgcgaacatt ttgattgaac ccgccggaca ggctgcgctg
     6541 cccgcgctgt ttagtgagag cagtaatccg ctttccggtc aggattttct tgatgaagcc
     6601 gagctgccga acatcaaaga tatcttctgg cgaaacaaca ttgtcgggtt tgcgccgatg
     6661 ctcggcggcg aagccagcgt ggagggagaa ccagtgcgga ttctgggcac tttcttcagt
     6721 cagccagtgg atattcctga tgaagaggga tatgagacgg ggcagaaaac ggtcagccca
     6781 tactggcagg tgaccggcga ctggccgcag gaacccgcag gcgcagagcc gcagacctta
     6841 gtcgggcacg cgctggcgcg gcaaatgggc tggaaacccg gtgataagct gacattacgt
     6901 accgaaggcg aagcagtgca ggtcaccgtc agcggtatcc tctccagcgg cggcgatgaa
     6961 gataaccaac tggtgatgcc gctaagcacg gtacagcacc tgctggggct ccccggaaaa
     7021 gtgcaggcga ttcgcgtctc ggcgctgacg gtgccggaaa acgaactgtc ccgccgcgcg
     7081 cgggaaaacc tggacgcgct caatgccgaa gagtacgacc tctggtattg cacggcctat
     7141 gtgtcgtcga tcgcgcatca gctggaagag gcgatttccg gtgcggaagt gcgcccggta
     7201 tggcaggtcg cggcgtccga aggggtggtg atcgataaaa ttcagttgct gatggcggtc
     7261 gttaccgtcg cggcgctggt ggcttccgca atggggatcg cctcgctgat gaccagcacc
     7321 attatggaac gcgccaaaga gatcggtttg atgaaagcgc tgggtgcgcg acagtggcaa
     7381 attatgctgc tgttctacct ggaagcggcg tccagcgggc tggctggcgg tgcgctggga
     7441 tgtattgccg ggtgggggct ggcgaaagcg attggcgtca tgctcttcga cgcgccgctt
     7501 aattttgcct ggattgtggt gccgtgcgta ctggtgattg cggtactgat tgcgttgatc
     7561 ggcacctggt tcccggcccg gcggattgcc cggctgtatc ctgtggaggt gctgtatggc
     7621 cgctaaacgt accatgctct ggctgctggt gtggcgggcg ctgcgtctgc gttttcagcg
     7681 ggtcagcgtg gtgtttgccg cgctgatggt gggggcgacc atcgtgacgg cgctttctgc
     7741 cgtctggttc gacattaaca ccaaaatgag cgaagaactg cgcaccttcg gcgcgaattt
     7801 ttacatcggg ccggggcacg gttccagtat gccgcagcag gagttgcaga gcattctcga
     7861 tcaggcgccg caggggctgg tgcatggcgc cagtccgtgg ttgtacggca tggcgcgtac
     7921 cgagctggaa aaagtggtga tggttggcgt gtggttcgag tcgctgcaaa aaatggtgcc
     7981 gtactggcag gtcaccggta gctggattgg cgtcagtttt gacgaccgta atgcgatgat
     8041 cggcgtcaaa ctggcggaac gcctcaacgt gcagccgggc gacagcatca cgctggtgga
     8101 tcacaatcag cgtaagaatt tgcagattaa aggcattgtg gagtctggcg atgcgacgga
     8161 caacatgctg atcgtcagcc tcgacgtggc gcaggcctgg ctgcatcagc cgggcaaaat
     8221 cagccacggc ttgctgagcg tcagtaacga tgtcggccag gtggagaact atgccagccg
     8281 gttgcaggcg cagtatcctg acctggagat tcgtccggtg cgtaaggtct ctgcttccga
     8341 agggcaggtg ctggataaaa ttaaaggtct gatgggactg gtctcgatcg tgattctggc
     8401 gctctcttcg ctgtgcgtga ataccacgct gatggcgatt gtcggcgagc gggcgcgcga
     8461 gtttgcgctg caaaaagcgc ttggttccag caacggcgat atcgtgcggc aaattctgct
     8521 ggaaaccagc atcatcgcgc tggcggcggt ggcgtgcggc tgggtgctgg gatatttgct
     8581 ggcgcaactg ttgggattaa cggtgtttaa tgccgccata tcactgcgtc tgccggtgtt
     8641 gcccattact ctggtgttgt ctttactggt cgccattctg gcggcgattg ttccggtgcg
     8701 acgcgcggta agcgtcgagc ctgctaaagt gctgaaagga gagtagcgcc atgtccgtta
     8761 cgcatatccc tcaggtggcg atcgaaaccc gccacttata caaacggttc ggtgacgtta
     8821 ccgccctgga tgacattaat ctgcgcattg cgcagggtga atttgtcgcc attatgggcg
     8881 cttctggttc tggtaaaacc acgctgatga atattctgac gtgccttgat acggcgacgg
     8941 aagggcaggt gtttcttgat ggcaccgatg ccgccgcgct ggatgaagag gggcgccgtc
     9001 gctttcgcgc cgagaaaatc ggcctggtat ttcagcagtt ccatttgatc ccgttcttaa
     9061 ctgcgctgga aaacatcatg ctcgcccagc attaccacag cgtggtggat gaagcggcgg
     9121 cgaggaaggt gctggaacag gtagggcttg ggcatcgtgt gacgcacctg ccgagccagc
     9181 tttccggtgg tgagcagcag cgtgtgtgta tcgcccgggc gctggtcaac gaaccgccgg
     9241 ttattttcgc ggatgaaccg acgggcaacc tggatgaaga gaatgagcag cgggtgctgg
     9301 atctgctcac cgatctgcat cgtcaggggc gcacgattgt gatggtgacg cacaaccctg
     9361 cgcttgggca gtttgccgac cgtattctgc ggctgcaaca cggtaaatat ctgggtgagg
     9421 aggccaatca acatgcgctg gcataatgtg ctgttactcg cggcggcgat cctgataagc
     9481 gggtgtaaag aagaaaagct ggcggtcggg gcaccggccc cggcgctggc ggtgtttgat
     9541 ctgcaaggcc aggagtcagg ccttgaacgc tggcaaggaa aggcggtgta cctgaatttt
     9601 tggtctgcgg gatgcggggg gtgcctggcg gaaatggaca cgctggaagc gctgagcaaa
     9661 aaatggggcg ataaagtggt ggttgtggcg gtcaataccg accctgaaac ggtcaggatc
     9721 aacgatctgc tggcaaaaca tcagataacc tatccggtgg tgcgcgatca gctcaacatt
     9781 acccaggaac gttatcaggt gatcggcacg ccgacctccg tactgattga ctccgaagga
     9841 cgcgttctgg agctacacca gggtatgcgt aagccgcctg agttagaggc cacgtttgcg
     9901 cgcctggcgg cgcgatgaac gacgcgtacg gagagatacc caatggcatt tatctggaac
     9961 gacgaaagcc ttgcgattct gcgggaaaac gcgggcatcc tcacgacaga gcagatagcg
    10021 caactgcttc acactaatat taccgccgtg cggaatatgg cctatcggct gaaactgagc
    10081 cttcgggtga cggcgtataa tcaccgacgc atagcgcagg ttcaggcgct ttatgcctct
    10141 gagacgttga gtctcaaaga gatcgcggcg aaaacggggc tgacggccag taccgtgcaa
    10201 tatatcgtgt acgtgaaatc aaaaaataag ccctacgcca ccacggaata tgtctctttt
    10261 gaaacggaga acgccgttca ttaccgcgtg caaaaagaat ttgtcgatac ggaacgttcc
    10321 ctgctggaca acatctctga taacacgcga tttcgtgaac tgtatctgac ggacggcact
    10381 ttttattgcg cccgcaatat taagtatgag gtttttatct cggagtaacc ggaacacgct
    10441 gacctgccgg ttatcttctg gaatctcccg ttttgcgaaa ccgttcccgg aaaaatgtca
    10501 tatttctgtc acactctatg aggattgata acaacaagag gttgtaggga tgaaaaaatt
    10561 gatactcgcc gccgctgtgg caggggctgt tttattgtcg tccgccgcgc aagcgcagac
    10621 aacgccggaa ggttatcaac tgcaacaagt gttgatgatg agccgacaca acttgcgcgc
    10681 gccgttggcc aataatggca gcgtgctgga gcagtccacg ccgaatcagt ggtcggagtg
    10741 ggatgtgccc ggcgggcagc tcaccacgaa aggcggtgtg ctggaaattt atatgggcca
    10801 ctacatgcga gaatggctgg cggagctggg gatggtcacg tcaggcgaat gcccgacgcc
    10861 ggacacggta tatacctatg ccaacagtct gcaacgcacc gttgcgacgg cacaattctt
    10921 tatcaccggc gctttccctg ggtgcgacat tccggtacat catcaggaaa aaatgggcac
    10981 gatggaccca acgtttaatc cggtgatcac cgatgactct gcggcgttta gccagaaagc
    11041 ggtgcaggcg atggaaaaag agcgcagcca gatgcagctt gacg
//
LOCUS       DAEEDS010000115        10929 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEEDS010000115 DAEEDS010000000
VERSION     DAEEDS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.115"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..206
                     /locus_tag="KLH32_003513"
     CDS             <1..206
                     /locus_tag="KLH32_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140202.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="KLH32_003514"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="KLH32_003514"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBE0140203.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2105..2821
                     /locus_tag="KLH32_003515"
     CDS             2105..2821
                     /locus_tag="KLH32_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0140204.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3277..3352)
                     /locus_tag="KLH32_003516"
     tRNA            complement(3277..3352)
                     /locus_tag="KLH32_003516"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3317..3319),aa:Asn,seq:gtt)
     gene            complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="KLH32_003517"
                     /pseudo
     CDS             complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="KLH32_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5085..5160
                     /locus_tag="KLH32_003518"
     tRNA            5085..5160
                     /locus_tag="KLH32_003518"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5118..5120,aa:Asn,seq:gtt)
     gene            complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="KLH32_003519"
     CDS             complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="KLH32_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBE0140205.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6250..7167)
                     /gene="nac"
                     /locus_tag="KLH32_003520"
     CDS             complement(6250..7167)
                     /gene="nac"
                     /locus_tag="KLH32_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBE0140206.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7495..7570
                     /locus_tag="KLH32_003521"
     tRNA            7495..7570
                     /locus_tag="KLH32_003521"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7528..7530,aa:Asn,seq:gtt)
     gene            complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="KLH32_003522"
     CDS             complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="KLH32_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0140207.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="KLH32_003523"
     CDS             complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="KLH32_003523"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBE0140208.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="KLH32_003524"
     CDS             complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="KLH32_003524"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBE0140209.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10454..>10929)
                     /gene="cobU"
                     /locus_tag="KLH32_003525"
     CDS             complement(10454..>10929)
                     /gene="cobU"
                     /locus_tag="KLH32_003525"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBE0140210.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3035 a         2782 c         2366 g         2746 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc
     1861 agatgcgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     1921 aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatataagat
     1981 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
     2041 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
     2101 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
     2161 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
     2221 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
     2281 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
     2341 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
     2401 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
     2461 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
     2521 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
     2581 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctgctg accttcataa
     2641 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
     2701 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
     2761 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
     2821 attatatttt aaagaaatct gtctttatgg cagatttctt taatctcata taattcttat
     2881 aaaaaatata atatttaact cgtcatattg cttataaaaa atccccgtcc ccagagaaat
     2941 aatatttatt aaaatggcag gtcttctttt tctgattacc gaagacaaag tggcaattac
     3001 gtatagtttc ccgataaaga cgcgacagct acgtcccgca taaggcattt ttctctttat
     3061 ctttgtacgg tacttcatgg aacagaattt ttgaccttgt gaatcgtgat atctgttggg
     3121 gaggggcaat ttgctcactg aagcgtgaga ctcgattaag cgcacgaaac acagaaatca
     3181 aaaaacccgg ccactttttt acaaagtggc cgggtaaaaa taatttttat tttttaactg
     3241 tcctgagtct catagagatg tctcaaaact aaaatttggc tcctctgact ggactcgaac
     3301 cagtgacata cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag
     3361 aacgaggcga atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa
     3421 atcgtttgct gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca
     3481 cacttttgcg gctcggatcg agggtatttc cacagccaac gtccagtaac cattcgccag
     3541 taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac accaaccaca
     3601 ccccagccaa gcatgattcc ctgcacataa ccgactacaa cccggcaacc ccacatgctc
     3661 aacatagaaa cccacatggc gtaacgggca tcacgagcac ccttaaatcc agcgggtagc
     3721 acccatgagg cggaccaaat aggcataaat aaagcattta gctaaatcag aatcacaacg
     3781 acgtgtttaa cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc
     3841 gttagccagg cgatggccgt taatccaaga gtggacagcc agaacacatg ccgcaactga
     3901 atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca
     3961 gaaccgagcg cacttccggg taagttgata agagccgcaa ttgagaacgc gataaaattt
     4021 ccggcaataa cgctggtccc catcccggca acgaacattt gggttaataa ccgaccactg
     4081 gtaaataaca ctgattcgac actcgctgga ataccaatcc ccatgacttc ccagataatg
     4141 ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca
     4201 atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccaac
     4261 ccagccccga ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa
     4321 tattcaggct accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc
     4381 cgctaccaat aagagtgatg gcagcggctg gataactgag tacggtcagc tccagataag
     4441 tcaacgccag tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgtt
     4501 cgccaaaatg atgaattagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg
     4561 actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt accgagacta aatgctacaa
     4621 cgacagtagt accaagatcg atagctgcaa aaaaagccat aatgaccata ttgaagctgt
     4681 ccgccaaccc cacgccggcc atcgcatctt ttcccagcca actgaccaga aaagtgctca
     4741 ggccccccat caacaggaca caggcgttct ccatgaagat aggaacagca agcggggtta
     4801 tctcgcgcta gaacaacact ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa
     4861 caacctggcg taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc
     4921 acatttcaaa tgatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc
     4981 tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt gggaaattta aaaatcgggt
     5041 ttgacaaaat atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt
     5101 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     5161 aatttctgaa aagcccgctt ttatagcggg ctttttgcta tatctgataa tcaatttcct
     5221 cttcactgtt ttccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa
     5281 attccagaaa gcgccaaaca tagttacgct gaagttgtcc tcgcttcagt cccaaccaga
     5341 cagtattagc atcgaaaaga tgccgcgtat ccaggcggat taaattctct tcctcttgtt
     5401 cgccgctgga ttgttcggca actaatccga tcccaagccc aagagcaaca taggttttaa
     5461 tgacatcaga atcctgtgca cttaatacaa tatctgccgg caaacctttg cgggtaaatg
     5521 cgtcatcagt gcgtgagcgc cccgtaattc cctgtcggta agtaattaac ggccacttcg
     5581 ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca
     5641 aactatggtg ccaacaaaac cacgggaagg cgacgagctg cgggtcatta ctcaaacgct
     5701 cgctggcgat accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg
     5761 aaccctggat tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct
     5821 ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat
     5881 cgttggtaaa caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg
     5941 caatgaccag taatgctttg cccggttcag tcatccccag cagtcgctta cctcgtcgaa
     6001 caaatatttc gatgccaaat tcatcctcca gttcccgaat atgacggcta acgcctgact
     6061 gtgaggtaaa aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc
     6121 ggattatctt tagttgttgg aaattcacgg taaactccgg gcagttcaga tatcccgtta
     6181 ttgttaaagt ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg
     6241 atataacgct tagctcacca attgccactg ccttttttcc atcactggag aactaatcac
     6301 tgacattaac aactctttca ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa
     6361 atttaaggac aaagagagac tcattgaagg agtggtaatg cgtgacatcc acccatttac
     6421 tgcgccacat aacgaacgcg cagccgattc aggtaatact gcaacgccca tgccgctggc
     6481 aatcgctgcg gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagccg
     6541 ccgtagggaa aaagcctcat caacacgaag tctaacagcg ctgtaatcac tggggagaaa
     6601 aaggttcatt tgcgcaatag cattaacatc aacgttttgc cccgggcaat cttgagttcc
     6661 taccaggaaa agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg
     6721 ctcataaatc accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc
     6781 actattttca tgaagataga taacgacctc cgggaattca gcgcgaaccg cctgtaataa
     6841 gggcatggtg atggatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga
     6901 taatgactga ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc
     6961 ccgtgcatgg gtatagataa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg
     7021 aatcaaaagc tgttgattta actcaccttc cagtatggca acctgctggc tgagcgctgg
     7081 ttgtgcgata tgcaatactt cagcagcctg ggtcaggcta ccaatatcta caatttttac
     7141 gaagtatttc aggcgtctga agttcatgtt gcctccggtt tttaagaatc ggcccaagtg
     7201 ccgccattac ttacaaccag tatgcaagat gcctgccagt tctattttgg cgttgatgcg
     7261 caagctaacc aactgtcaaa taagagatta tgatagattc gtcattggcc ctttttatca
     7321 gcagtcgcat tcacctgccc tataaaagga gggtatgcac cacgatggtt cattacccaa
     7381 taagattgaa agctcaccac tttgttgaaa ttggcagcaa acaaacaaaa aaaatgcatt
     7441 tcaccctttg acatcaccat gcactgccat taatatgcgc cccgttcaca cgattcctct
     7501 gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc
     7561 agaggagcca aattcaaaaa agcctgcttt cgagcaggct ttttactttc taattaccaa
     7621 cgctcttaaa acatctgtct tgaaccagaa ctaatttgca caggcattcc cgatcgatgt
     7681 tgcaacgcag catttgcgcg atttacatca acttcttgcc cgttgataaa cgcccgcaaa
     7741 gatggggtta ccggcaatgg cacttttcgg tcagactcat attctgcacg attgcgcgac
     7801 agcggctcat gaacttccag ccactttgaa ccatccggtt ccgttgtgta tttcactggc
     7861 tgatcaataa tttgtacacg agtgcctaca ggaacattat caaacagata tttgatatcg
     7921 tcattacgca gacgaataca gccctgactt acacggagcc caataccaaa attggcattg
     7981 gtaccgtgga tggcatacaa cctgccaata taaatcgcgt acagccccat ggggttatcg
     8041 ggccccgcag gaacaaatgc gggcaaactc tcccctcgtt tcgcatattc gcgccgggtg
     8101 ttcggcgttg gcgtccaggt tggtgcttct tgtttacgtt caacggtagt cacccagtta
     8161 cgcggggttt ctcgcccagc ctggccgata ccaataggaa agacttccac tgtattactg
     8221 tctggcggat agtaataaag acgcatttca gcgacgttaa ccacaatccc tttacgaaca
     8281 gtggcgggca aaatcagttg ctgcggaatg gtgagttgtg agccaggctt cggcaaaaaa
     8341 acatcagcgc ccggattcgc ttccagcata ttacttaacc cttgcccgta ttgtgcggca
     8401 aaagtctcca gcggctgagt attgtgatca ggaacagtta cagtaagcga ctgccccact
     8461 aaacggctac cctctggagg taatggataa gttaccgcca ggctattttg gctggcaaaa
     8521 agcagagcaa atgagcaaag aatatttaca cgacgcatca tgtccctttc ctaagtcgcg
     8581 aatactatcc gataagtata gcttttatca gacttttcgt ttttagctgt tcaaatcaga
     8641 agtcgtattc cccggtagaa caatattact ggcagcaagt tcgcccatgt tgttgtatat
     8701 cgcacaagca gcttcgatga tggacatcgc cagagctgca ccactcccct cacctaaacg
     8761 catatccata ttgagataag gctccagccc caaatgcgag agcgctatac gcgcgccttt
     8821 ttctgccgac aagtgagaag gaatgagata cggtttgatt gcaggagaca tctggcaggc
     8881 tgcgagcgca gcagcataag aaagaaatcc atccagcaac acgggtaaac cgcaggaagc
     8941 agcacctaac atcactccag ctattccgac caaatcaaat ccacccactt ttgccaggac
     9001 atcaacacca tcctgaggat ttggttgatt caacgtaatc gcccgacgca caacatcaat
     9061 tttattagcc agtttatctg tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc
     9121 ccggccagtg attgtgctga ctattgccgc tgccggtgtc gtgtttgcca tccccagttc
     9181 acctacacca aacagcgtga caccgttttt cgccagctcc cgcgtataac atatgacgtc
     9241 caaaagcaac ttttcagcct gacggcgact cattgccgga gctgaagcaa tattgccgct
     9301 acctcgtgcg acacgcatgt tgataagccc ggggataggc tcagcagtat caataccaac
     9361 atcaattacg tggacgttag cgcccgcttg tgctgccagc acacacacgc cggttgttcc
     9421 acgggtcata ttttcagcct gtatggctgt cacttctttt ggagaaatag cgaccccttc
     9481 ctcccagacg ccgtgatcgg cacacataac cagtaccgct tttttgccca catgcggtat
     9541 gccattcacc cccggcattc ctgccagttg tatggcaagc gcctccagct ttcctaggct
     9601 accaacaggt ttgaataacc cgtcaacatg ccgttgtgca cgcgacatag cggcagaatt
     9661 gatggctggg atcgtattca gtaaatcggc aattatttgc atgtcacgtt tctttatagc
     9721 agtgccagca ggaagaccag ttcaccaagc tctatggctg ctcccagcgt atcgcccgtt
     9781 tgcccgccca gcgtacgttt gagaagctga ccgaggatga aaatcgccac catcgtcacc
     9841 accatagcag ccacaccatg catacccagc aataatacgg cagcaaaaat agcggctaag
     9901 ccgagtgtga cgcaggtttg tcgcccatca attttgccaa tgaacacatt gccaagccct
     9961 tcttcccgcg cataacgatg acgatacatc aataatgcgg cagtcccgcg gctgaccgca
    10021 catgctgccg ccagcgatgc aaggatcggt tcgccacgca gagccaactc gcttaacacc
    10081 agaatctttg ccagtaccac aaaaatcaat gccagaccgc cgtgggtgcc taaacggcta
    10141 tcacgcatga tttccaacat gcgatcgcga ctacgtgcag aaaatacgcc atcacaggta
    10201 tcagcgaggc cgtccagatg gaattccccg gtcatcagcg ccagcacaag tacgctaaac
    10261 agtgccgcca gtggtgcgcc acaccatgcc tgcagcacca tgaagaccag cccgctaatc
    10321 gcgccaagca ataatccaat caaaggaaaa gtaataatac cgcgagaata atgctcgaaa
    10381 tccagtccct gggaccagcg acgcggtaca ggcagacgcg taataaatga gagcatcgcc
    10441 caaaataatt tgctcatttg atttttactc caatacccga aaccaccagc catacttcat
    10501 ttgccgcagc ggccaactgc tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca
    10561 gacgactctc cggcacaatc cccattccca cttcgttagt cactaataca acctttgcgg
    10621 ggcaacgttg gcaggcagca atcaacgact gaatctcagc attaatcgcc tgttccatcg
    10681 cctgataatc ccattcatca gggtctttat cgccgccata atcaaacaac agattagtca
    10741 ccattgtggt aacgcattcc agcaacacag cctcattcgg gttaatgtct gcatgaatta
    10801 attcatcaag atgttgccag cgctccactg tgcgccagtg ctccgggcgg ctttgccgat
    10861 gatgttctat ccgtgcagcc atctcatcat caaggatttg cgaggtagcg atatacagaa
    10921 cctgtgaag
//
LOCUS       DAEEDS010000116        10901 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEEDS010000116 DAEEDS010000000
VERSION     DAEEDS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.116"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="KLH32_003526"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="KLH32_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBE0140211.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KLH32_003527"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KLH32_003527"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBE0140212.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="KLH32_003528"
     CDS             complement(2271..3686)
                     /locus_tag="KLH32_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0140213.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KLH32_003529"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KLH32_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBE0140214.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KLH32_003530"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KLH32_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KLH32_003531"
     CDS             complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KLH32_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBE0140215.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         2510 a         3070 c         3003 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta a
//
LOCUS       DAEEDS010000117        10747 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEEDS010000117 DAEEDS010000000
VERSION     DAEEDS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10747
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.118"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(133..1482)
                     /gene="lysC"
                     /locus_tag="KLH32_003532"
     CDS             complement(133..1482)
                     /gene="lysC"
                     /locus_tag="KLH32_003532"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBE0140216.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2007..3656
                     /gene="pgi"
                     /locus_tag="KLH32_003533"
     CDS             2007..3656
                     /gene="pgi"
                     /locus_tag="KLH32_003533"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBE0140217.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4118..4360
                     /locus_tag="KLH32_003534"
     CDS             4118..4360
                     /locus_tag="KLH32_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140218.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4474..5112
                     /locus_tag="KLH32_003535"
     CDS             4474..5112
                     /locus_tag="KLH32_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBE0140219.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5109..5846
                     /locus_tag="KLH32_003536"
     CDS             5109..5846
                     /locus_tag="KLH32_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBE0140220.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5846..7942
                     /locus_tag="KLH32_003537"
     CDS             5846..7942
                     /locus_tag="KLH32_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBE0140221.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7989..8267)
                     /locus_tag="KLH32_003538"
     CDS             complement(7989..8267)
                     /locus_tag="KLH32_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140222.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8268..8411
                     /locus_tag="KLH32_003539"
     CDS             8268..8411
                     /locus_tag="KLH32_003539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140223.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8537..8947
                     /gene="psiE"
                     /locus_tag="KLH32_003540"
     CDS             8537..8947
                     /gene="psiE"
                     /locus_tag="KLH32_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBE0140224.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8991..10466)
                     /gene="xylE"
                     /locus_tag="KLH32_003541"
     CDS             complement(8991..10466)
                     /gene="xylE"
                     /locus_tag="KLH32_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBE0140225.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2727 a         2741 c         2778 g         2501 t
ORIGIN      
        1 gtttttctgc tcattattag ctccttctga tgccattcta tttcaggaag gagtgtccgt
       61 taaactcagg ctacctcata gtgacaagaa aatcaatacg gcccgaaata tagcttccag
      121 gccatacagt atttactcaa acaaattact atgcagtttt tgcaccacct gctcggcatc
      181 ttcgccgggc accaggaagc acaggttatg gctggatgcg ccgtaacaaa tcatgcgaat
      241 gttgaacggt tccagtacgc cgaatacctc tttgccaacg ccgcaggctt ttgacaggtc
      301 attgccaatc aacgcgacca gcgccagacc ttcttccacc tccacccggc acagtgcgga
      361 aagctccatc agcagagatt gcgtcagcaa cgtatcgcca gtggaggttg aaccggtggt
      421 atcaagggtt aatgccacgc tcacttctga cgtggtgatt aagtctaccg aaatattatg
      481 ccgcgcgagg atgccgaaaa cttccgcgag gaagccgcga gaatgcagca tattcaggct
      541 gtgcaaagtg agcagagtct gattgcgacg aagcgccagc gcgcggaaca gcggcggatt
      601 ttcagtttta ttgcacacca gcgtaccacc tgcgcgtggg tctttgctgg agccgacaaa
      661 gaccgggata tcgctgcgta ctgcgggtag caacgttgcc ggatgcagta cttttgcacc
      721 aaaagttgcc atctctgccg cttcggcaaa cgcgatttca tcaatgcgtt ttgctgcgga
      781 aactacgcgt ggatcggtgg tgtagatgcc cgggacgtcg gtccagatat caacacgaga
      841 tgcgtgtaaa gcctccgcca gcaaggctgc cgtataatcg ctgcctccac ggccaagcgt
      901 cgttgtacga cctttatttt cgctaccgat aaatccctgg gtgatcacta agccttcatt
      961 gaggcgtggg agcagctgca gcgcggccag ttccgccagc gcggctacat ctggctctgc
     1021 acgaccaaat cggtcgttgg tacgcatcac tttacgtaca tcaaaccact gtgcctgaac
     1081 atcgcgttcg cgcaggattt cgacaaacag cagggtcgac atcagctcgc cgtggctgac
     1141 cagctcatct gtcagcgccg gagacgttgc cagcgccgcc gcttctgcca gaacagtaat
     1201 gttctccagc agacgttcaa tctcttcacg gataacgttc gggtaacgca gacgttccag
     1261 aatggcaaac tggatgttgc gaatagcgtc gagtttttcg aatcgctcgc caagttccag
     1321 cccttccgct aaagcgacca gcagattagt gataccagca gaagccgaga ggactactaa
     1381 acgcacgttg gcatcagaaa gcacaatatc agcgctgcgg ttcatggcgt caaaatcagc
     1441 tacgctggta ccgccaaatt tggagacaac aatttcagac ataactacct cgtgtcaggg
     1501 gatccatttt cagccttggc acaagggaag agcggaagac gggtgggcgc agagcgatac
     1561 ttcgctacta ttttcaccca gaagtgctcc accacttgcg aaacgcccga ctgcgaacgc
     1621 ttctggtgac aacccagggg attcagcccc tgtagccgat gatgaacgtg gccagccgtt
     1681 caatcacctc ggcgatgcac cccctcaggt gttatcacag gactggctcc tccaacaccg
     1741 ttacttgggc aacgcgcctc ttctggcctg cgctagcgca ggtagtacat ttataaataa
     1801 agggtgagcg gggcggttgt caacgacggg gtcatgcgga tttttcatcc actcctggcg
     1861 gtcagtagtt cagctaataa atgcttcact gcgctaaggg tttacactca acattacact
     1921 aacggcacta aaaccatcac atttttctgt gactggcgct acaatcttcc aaagtcacaa
     1981 ttctcaaaat cagaagagta ttgctaatga aaaacatcaa tccaacgcag accgctgcct
     2041 ggcaggcact acagaaacac ttcgatgaaa tgaaagacgt tacgatcgcc gatctttttg
     2101 ctaaagacgg cgatcgtttt tctaagttct ccgcaacctt cgacgatcag atgctggtgg
     2161 attactccaa aaaccgcatc actgaagaga cgctggcgaa attacaggat ctggcgaaag
     2221 agtgcgatct ggcgggcgcg attaagtcga tgttctctgg cgagaagatc aaccgcactg
     2281 aaaaccgcgc cgtgctgcac gtagcgctgc gtaatcgtag caataccccg attctggttg
     2341 atggcaaaga tgttatgcca gaagtcaacg cggtgctgga gaagatgaaa accttctcgg
     2401 aagcgattat ctccggtgag tggaagggtt ataccggcaa agcaatcact gacgtagtga
     2461 acatcgggat cggcggttct gacctcggcc cgtacatggt gaccgaagca ctgcgtccgt
     2521 acaaaaacca cctgaacatg cactttgttt ctaacgtcga tgggactcac atctcggaag
     2581 tgctgaaaaa agtaaacccg gaaaccacgc tgttcctggt agcatctaaa accttcacca
     2641 ctcaggaaac catgaccaac gcccatagcg cgcgtgactg gttcctgaaa gcggcaggtg
     2701 atgaaaaaca cgttgcgaaa cattttgcgg cgctctcaac caatgccaaa gccgttggcg
     2761 agtttggtat tgatactgcc aacatgttcg agttctggga ctgggtcggc ggccgttact
     2821 ctttgtggtc agcgattggc ctgtcgattg ttctctccat cggctttgat aacttcgttg
     2881 aactgctttc tggcgcacac gcaatggaca agcatttctc caccacgcct gccgagaaaa
     2941 acctgcctgt cctgctggcg ctgattggca tctggtacaa caatttcttt ggtgcggaaa
     3001 ctgaagcgat tctgccgtat gaccagtata tgcaccgttt cgcggcgtac ttccagcagg
     3061 gcaatatgga gtccaacggt aagtatgttg accgtaacgg taacgttgtg gattaccaga
     3121 ctggcccgat tatctggggt gaaccaggca ctaacggtca gcacgcgttc taccagctga
     3181 tccaccaggg aaccaaaatg gtaccgtgcg atttcatcgc cccggctatc acccataacc
     3241 cgctctctga tcaccaccag aaactgctgt ctaacttctt cgcccagacc gaagcgctgg
     3301 cgtttggtaa atcccgcgaa gtggttgagc aggaatatcg cgatcagggt aaagatccgg
     3361 caacgcttga ctacgtggtg ccgttcaaag tgttcgaagg taaccgcccg accaactcca
     3421 tcctgctgcg cgaaatcact ccgttcagcc tgggtgcgtt gattgcgctg tatgagcaca
     3481 aaatctttac tcagggcgtg atcctgaaca tcttcacctt cgaccagtgg ggcgtggagc
     3541 tgggtaaaca actggcaaac cgtattctgc cagagctgaa agatgataaa gaaatcagca
     3601 gccacgatag ctcgaccaat ggtctgatta accgctataa agcgtggcgc ggttaatcat
     3661 catgcgtgcc tgatgccgat atgtaggccg gataaggcgt ttacgccgca tccggcaacc
     3721 ggagcctgat gccaatatcg acattaatgc atagtatgag gagttaaata accttaaaaa
     3781 caatgccgac aataagtcgg cattgtttta tcagataaat ccccttgtct gtgatttaac
     3841 ggaaatcata ccgtgaggtt aatcctaaaa tagattttta atcgttgttt atttcggaga
     3901 atacgcagat taattacttt tgtttttatt ttaagcttat gatttttatt gttatttaaa
     3961 tataagttga aacttatatt tgatattcat tccaattatc ctaaaactcc atcgctaatt
     4021 ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg aaatgttttt
     4081 gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc tgtatggcat
     4141 ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac aggtgggcga
     4201 agcggcagag tcggcagcaa cgtcggtttc ggcggggagt tcctccgcga ccagcgtcag
     4261 caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg atggttctaa
     4321 taccgggacc atcacaacca cgaccaccag tacccagtaa taaagtatgt atccccaaaa
     4381 taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata accacactcc
     4441 ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact cattcttatc
     4501 tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc actgtgggac
     4561 agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa tatgccctac
     4621 gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct ggccttcgct
     4681 gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac acaacatggt
     4741 cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa ccttgccgcc
     4801 gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg cacgatgggc
     4861 tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa atgggatggc
     4921 accgataccg tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga cgaagaagtc
     4981 tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg gcaaattcgc
     5041 cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct catcaaggcg
     5101 gcaaaacaat gattaaacaa actattgtcg cgttgctttt gagtgtggga gcgtcatcgg
     5161 tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc aaaacgctaa
     5221 cgggcgcaga gcatttaatc gatctggtag ggcaacctcg actggcaaac agctggtggc
     5281 ctggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag cagcaggcgt
     5341 tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc gccgccatta
     5401 acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa atcaatcttg
     5461 atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc aactacacgc
     5521 tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc catcctggca
     5581 atcagccatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag agcctgctca
     5641 gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg caaaaagcgc
     5701 cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt atttatgttg
     5761 gcctcgcaga ttccgtctgg agtgagacgc ctgatgccct taacgccgac attcttcaga
     5821 ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct tactggcgct
     5881 gggcattagc acagcttgct acggcgaaac atatcctgcg cccattggtc cgtcgcagtc
     5941 ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac gggaagggga
     6001 gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt cagtgcaact
     6061 cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc agtacagcag
     6121 cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc tcaaactgcg
     6181 tttgtgggaa gagagttact ggctgccgca agtggcggtt ggtgcgcggg atatcggcgg
     6241 tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc cgttcgattt
     6301 tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtaaaaa atccgctctg
     6361 ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg gatctatcga
     6421 cggtagccag atgttccacg gtcctgcctc actgtttggc ggcgtggaat accagacgcc
     6481 ctggcaaccg ctgcgtctga aactggagta tgaaggtaat aattatcagc aggattttgc
     6541 cgggaagctg gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg ttaccgattg
     6601 ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg tcacgctgcg
     6661 caccaacttt aacgatctgc gcccgtccta catcgataac gcccgcccgc aatatcaacc
     6721 gcagccgcag gatgccattt tgcagcattc ggtagtggcg aatcagttaa cgttgctgaa
     6781 atacaacgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc tgtatgttac
     6841 cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcta atcggatcgt
     6901 gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acggaaaatc gccttaacat
     6961 gccgcaagtg acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg ccggagagcc
     7021 gttgggccac gaaacgacgc tggcgcacaa acgcgtcgag ccagtggttc cgaagtccac
     7081 cgagcagggc tggtatatcg acaaatcacg ctttgatttc catatcgatc cggtgctgaa
     7141 ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga tgggaacggc
     7201 agatttgtgg ctgacggacc atctactgac caccggcagc ctgtttgcaa atcttgccaa
     7261 caactacgac aagtttaact acaccaatcc accgcaggac tcgcacttac cgcgcgttcg
     7321 tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg aataacctgc aagccaacta
     7381 cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc tcgaaaccat
     7441 gtttggcggt gcgggggccg aagtgttgta tcgtccgctg gacagtaact gggcgtttgg
     7501 tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata tgatgaaatt
     7561 caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat ctttcgccca
     7621 ggacgtgttg gtgaaagcca gcgtcgggca gtatctggca ggggataaag gcggcacgct
     7681 ggagatcgct aaacgctttg acagcggcgt ggtggtgggt ggctatgcca cgatcaccaa
     7741 cgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg tctcggtacc
     7801 gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct ggacgccgct
     7861 gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca tgaccagcga
     7921 ccgtagcgta aatttccgct aagtcatggg aaaggtgcca gttttcgcac tcactggcac
     7981 cctcattctt aatgacctgt tgagccatca ggaacgcagt tcacccccgg tttacgatct
     8041 ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg caggtctggt
     8101 tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag gtcggcttta
     8161 tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa catcaaagca
     8221 gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg ttccagagga
     8281 acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg gtgatttttg
     8341 aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg gcttatccgg
     8401 cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg ttgaacaaaa
     8461 catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag ctcgccactg
     8521 tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta tctccaccat
     8581 tttgcagacc gtactcaatc ttggcctgct gtgcctcggc ctgattttgg ttgtcttcct
     8641 cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa ccagcaaata
     8701 tgagctggta gaaggactgg tggtttactt tctctatttc gaatttatcg cgctgattgt
     8761 gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata ttgggatcac
     8821 cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtgt tgatctactc
     8881 ggctgcgatc ctgttactgg tgatcaccct gtggctgtgt aattcgaaac ggctgaagcg
     8941 ggagtaaaaa gacagcacgc cgaaatggcg cggcgtgctg gacaggaaga ttacagcgta
     9001 gcagtttgtt gtgttttctt cgtttccggt tcccagagcg cttccagctc ctcaagggtt
     9061 ttacctttgg tttccgggac aaatttccac ataaacagtg ctgccagaac gcccatacaa
     9121 ccgtaaatcc agtaggagaa accgttgtgg aaatgggcca ccagccagga gtttttgtcc
     9181 atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc caccgcgatt
     9241 gccagcgctt taccacgaat agcattcggg aagatttccg acagcagtac ccagcatacc
     9301 ggaccccagg acatggcaaa ggcggcaaca tagaacagca tcgacagtag cgccacaata
     9361 cccgatgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc cattccgagt
     9421 gcgccgataa tttgcagtgg cttacgacca aatttatcca cagtcataat tgccagtacg
     9481 gtaaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat atccgtgctg
     9541 gcccccagcg ttttgaacac ttccggcgcg tagtacagca ccacattgat gccgacaaat
     9601 tgctggaaga tggagagcat aacgccgatt acaatcacgc ccacgccaaa catcagcaga
     9661 cgaccaccgg ttttgcggcc atgatccagg gagtgtttaa tttcctgtac tgcctgagtt
     9721 gcaagcgtgt tgcccataat tttgcgcagg atactttccg tctgttcttg cttgccgcgt
     9781 gacatcagcc agcgaggact ttctggcacg gtatacagca gcattaagaa cagcagtgca
     9841 gggatacatt ccgaggcaaa catataacgc ctgccgtcag tattcagcca gctggcatca
     9901 ccggaacggg caataaaata gtttacgcag taaactaaaa gttgcccgaa aataatcgca
     9961 aactggttaa atgaaaccag ttttccgcga atatgagctg gagccagttc cgcaatatac
    10021 attggtgaaa gcattgaggc taaaccaacg ccaataccgc caataatacg ataaataaca
    10081 aattccggga cataacctgc cagataaata ggtactgtgt tgtccgggtt tatagaggta
    10141 aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac agcagaaatc
    10201 ttaagtgaat cacgacggcc gaagcggtta ctgcaataac caccgagggc accgccgatg
    10261 atgcaaccaa tcagagcgct ggctacgcaa aatcctaaca gggagttggc agcggattca
    10321 cttaagtttt gtggagcaac aaagacggta ttgagtgact caacagtacc ggaaataacg
    10381 gcggtgtcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat cgaaaatata
    10441 taactggaat tatactgggt attcattcag acctgcctta gaccattctg atcttttatc
    10501 agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt acaatgcgtt
    10561 tacgtaatgt cttttctgtg atcttaattg tgataattat caaaaattga tgaaaacaat
    10621 actattgccg tgactcagag cacgaaagag aattatcgta agtgggaaaa caaataacgt
    10681 aaaaataata agttctatga tgggaatatt aaccggcgaa cgattcagat tgcagacgaa
    10741 agaaaaa
//
LOCUS       DAEEDS010000118        10740 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEEDS010000118 DAEEDS010000000
VERSION     DAEEDS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.119"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..213)
                     /locus_tag="KLH32_003542"
     CDS             complement(<1..213)
                     /locus_tag="KLH32_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBE0140226.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSS"
     gene            704..835
                     /locus_tag="KLH32_003543"
     CDS             704..835
                     /locus_tag="KLH32_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBE0140227.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1095..2090
                     /gene="pgl"
                     /locus_tag="KLH32_003544"
     CDS             1095..2090
                     /gene="pgl"
                     /locus_tag="KLH32_003544"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBE0140228.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2131..3084)
                     /locus_tag="KLH32_003545"
                     /pseudo
     CDS             complement(2131..3084)
                     /locus_tag="KLH32_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3268..4320
                     /locus_tag="KLH32_003546"
     CDS             3268..4320
                     /locus_tag="KLH32_003546"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBE0140229.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4396..5829
                     /locus_tag="KLH32_003547"
     CDS             4396..5829
                     /locus_tag="KLH32_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBE0140230.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6012..8192
                     /locus_tag="KLH32_003548"
     CDS             6012..8192
                     /locus_tag="KLH32_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBE0140231.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8333..9616)
                     /locus_tag="KLH32_003549"
     CDS             complement(8333..9616)
                     /locus_tag="KLH32_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBE0140232.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9751..10632)
                     /locus_tag="KLH32_003550"
     CDS             complement(9751..10632)
                     /locus_tag="KLH32_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0140233.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2636 a         2471 c         2675 g         2958 t
ORIGIN      
        1 cgaggaaagc gccaaggctc gcaggtccag tttatgcccc ttagaggtaa gacatgcacg
       61 aagtcttctt accgcctcag tacgctgttc ttccggtaac ccctgtttct cccaatcatc
      121 ccattcggac aaataatggt tgggggatgt aattgtgctg atatgtggaa ataaacagga
      181 tgaaatgttt ctgagcatat taatttctcg catatggata tggaattaga ttgtgaataa
      241 attgtattta atgtatctct cctaatgagg agggtgtttc agaatattcg ccataaggga
      301 atgagtgtta tatcatgctt tctaccctgg taagtatatt tgcttttttc gtatatttgg
      361 ttcatctttt tttgatgttt tacatattca caatgcagag gatttatacc agaggacatt
      421 agtcttgagt aagatactac aacatctcca actattttag ccagtctgac tacttgactt
      481 ggtaatattt gttgctaata atcatgtata aaataagctt atatacatag taacttcagc
      541 aaaggattta gtaatgatca aaatcatatc tcaatgtact gtttttttct ttaaaaaaac
      601 agtacattct attttataat gactgtgtat tcccatggaa ggagatcacc caattctccc
      661 ccccgacttt tgacgcagtg tcttgttaac agggggattg accatgctta ttggctatgt
      721 acgcgtatca acaaatgacc agaataccga tctacaacgt aatgcgttta tttgtgccgg
      781 aagtgagttg attgttgcgc gaatgggaag ttggccagaa gttgttatgg catagtgttt
      841 acggtcgttg atacaggtaa acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa
      901 aatatctgtg gcattgtcaa attcgtgtcg gtgactaata ttttagtaat atgccattcg
      961 aaatggagtg ctaacttatt gatttaattg atgtgcgata ttgtatttta tcgtgttttg
     1021 ttgtctgttt tttatccagt aagtgttgtt atatgtatga aagataaggg atttatcatt
     1081 tttgaggtgt gttcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc
     1141 acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg
     1201 ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc
     1261 ctgagtttcg cgtcctggcg tatcgtattg ccccggacga tggcgcactg acctttgccg
     1321 cagagtctgt gctgccgggt agtccgacgc atatttccac cgatcaccag gggcagtttg
     1381 tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc
     1441 cagtgggcgt cgtcgatgta gtcgaggggc tggacggttg ccattccgcc aatatctcac
     1501 cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc ctgtttacgg
     1561 tcagcgatga tggtcatctg gtggcgcagg accctgcgga agtgactacc gttgaagggg
     1621 ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt
     1681 taaacagctc agtggatgtc tgggaactga aagatccgca cggcaatatc gaatgtgtcc
     1741 agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata
     1801 tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagtctg attaccgttt
     1861 tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc
     1921 agccgcgcgg cttcaatgtt gattacagcg gcaagtatct gattgccgct gggcaaaaat
     1981 ctcaccacat ctcggtatac gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc
     2041 gctatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt
     2101 aaccggcgca gtctctcctg cgccggtgta ttaacttatc tcctgtaacg catgtctctg
     2161 gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa
     2221 ggaaaaaaag cgttcagctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa
     2281 tgtccgacgt ataacgggtt ccaccagtgg gataccaata atcgaagaat agggcgcatg
     2341 gggcattgcc agaccaggaa gcgccgaaat ccccagtcct gcctccacta gtcctaatga
     2401 cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg
     2461 ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt tgtaacccac
     2521 taactcctgc cattctacaa gctacttttt ggccaatgga tggtcacgcc gacaggccag
     2581 cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga cgttgttcat
     2641 gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg
     2701 ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg
     2761 taaaaaataa aatactgcgg tcggaataca cgctaatgtc accatatcac gatgataagc
     2821 attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc
     2881 cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac gctcaaatag
     2941 ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat
     3001 attgagtaat ttagcggcat tgttaaatga actggactct gccagtatga caaatgcctt
     3061 catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg attttggggg
     3121 gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa aaatgttatt
     3181 aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata tcgcaataag
     3241 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
     3301 ggaacctcga gggccgcgtt cctgttagcg gaacatttac ccgaagatca aacgcagcgc
     3361 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
     3421 ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga tccgcgtgct
     3481 gatgtcgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga taccacgcct
     3541 aactgcggca atatgctgtc tggcgtaggg gcattcgcca ttgaaaatgg tttgattgca
     3601 gcgacttcgc cagttacccg tgtacgtatc cgcaacgtca atacgggtac gttcatcgaa
     3661 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagcgccag aattgacggc
     3721 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg aacgaaaacc
     3781 ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt
     3841 atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat
     3901 gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgg
     3961 ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct
     4021 gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
     4081 tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg
     4141 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat atggcaatat caatattgaa
     4201 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
     4261 cgcgcatctg ttattcggac aaccagaaaa atattttccg gtgaagttta tcttccctga
     4321 aaaaattcgt tgtcaggata aggacaatca ataaaggact tctgtatgag tcatacagaa
     4381 agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc
     4441 ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt
     4501 tttggtattt acctggcggc cattgtgggt ctggttatca agcctttccc ggaacctgtc
     4561 gtactgttaa ttgccgttgc tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg
     4621 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
     4681 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacttat tgcctatctg
     4741 ctgattggta aaatcggtaa caccatgctg ggtctgggtt acgttacggt attcctcgat
     4801 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg
     4861 atcatcaaca gcgtggcgat ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
     4921 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
     4981 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
     5041 attagctggg gtggatgggc gctggccgcc ggattgccgg gcatcattat gctgctggtc
     5101 accccgctgg taatttacac catgtatcca ccagaaatta aaaaggtgga taacaaaacc
     5161 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
     5221 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
     5281 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
     5341 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc
     5401 ttaagctcct tattatcgaa agttaaattt ttcgaatggt tagctgaagt ctttaaaaat
     5461 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatc
     5521 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt acctgtattt
     5581 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     5641 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     5701 ggcgtgggtt acaacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
     5761 accttcttgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     5821 atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     5881 ccagtcactt acttatgtaa gctcctgggg attcactcac ttgtcgcctt cctgcaactc
     5941 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
     6001 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     6061 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagcaaaa aaaggcacta
     6121 ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa cttaaaatta
     6181 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     6241 cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     6301 gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg gcagctcagc
     6361 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
     6421 tgatggctag cgggggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
     6481 tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg cttaatgaca
     6541 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     6601 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
     6661 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
     6721 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
     6781 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
     6841 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     6901 caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca ctgaaccaga
     6961 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     7021 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
     7081 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     7141 gtcaatcctg tggcaatgac accttctcgc tggcggttta cccgtcatca cagccggtgt
     7201 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
     7261 gaaccgcatt ctgtggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     7321 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gccaatgggc
     7381 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
     7441 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
     7501 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
     7561 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
     7621 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtcac caccaccgac gaactgattc
     7681 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcgggg aatgtcagcg
     7741 agctgacaga ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc
     7801 tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg
     7861 aacaggcggc gagctgccag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg
     7921 cgactaaacg ctaccgttct aacgtcatca actgggggat gttaccgctg cagatggcgg
     7981 aagtgccaac ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg
     8041 ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa
     8101 ttacgcttta catggaaagc ctgacggctg aagagcgcga gattatcaag gcgggtagtt
     8161 tgattaactt caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt
     8221 cggcacttat tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa
     8281 ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct
     8341 tcgcctctgc aaccactttg ctgcccacgc cgcggttatt gtattcccac atgcggttgt
     8401 agttagtgtc attcagattg cgctgtattt cgtcgttatc atctacgctg ccggtattac
     8461 ccgcaaacgg acgattagag atcaccgcat cggcccacgg tttagccgtg ttaaaacctt
     8521 cgttgatggc gctatcacgg atcaccacct gaccgttggt attagcatca acatccagcg
     8581 agcggcccag ttgcgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga
     8641 aaccgtaata gatgttagac agcgtagccg gtgcaaacac atatgcttct tgctgggtac
     8701 gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga
     8761 tatccacatc cccttcaata tagctgttgg tcaccagcgt acgcggctga cggttggttt
     8821 ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt
     8881 taacgttgtt aatctgtact tggtcaccgt cagtacgcag tgctaccgcc ggatggttac
     8941 ctgcatctac gctatcgccc agcgtgtttt cgatggtcag attttgcagt tgcagtccat
     9001 tgttttgtga ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct
     9061 ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga
     9121 cgtcgtgacg ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat
     9181 caatcggttt ttcacctgta ccgtacagag tgattccacc tggagcagca gggacataca
     9241 ccgttccctg atactcccca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga
     9301 taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg
     9361 ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag
     9421 cacctggtgt cagggatgca aaatagtgtt gcgcatcgaa attcagcgct tcttttgccg
     9481 acagaatcgg gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg
     9541 gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa
     9601 ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc
     9661 ctgctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
     9721 aacaggtcat tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct
     9781 gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg
     9841 agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg
     9901 aaaggtaggt ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata
     9961 ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc
    10021 gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac
    10081 atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgctcga cataaagata
    10141 tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt gcccggtaac
    10201 aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg cttgataaat
    10261 tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg ctgcgcgagt
    10321 ggcagcgacc gggtttgttg ttatatggcc ttcagctatt gcctctcgga atgcatcgct
    10381 cagtgttgat ctgattaact tggctgatgc cgccttgccc tcgtctatgt atccattgag
    10441 cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca gccccctcct
    10501 tattgcttta attttgctca tgtaatttat gagtgtcttc tgcttgattc ctctgctggc
    10561 gaggattttt tcgtagcgat caagccatga atgtaacgta acagaattat cactgttgat
    10621 tctcgctgtc agaggcttgt gtttgtgtcc tgaaaataac tcaatgttgg cctgtatagc
    10681 ttcagtgatt gaggtgtact ggcaatagcg gacactacca tttgttcttt ttttaagcag
//
LOCUS       DAEEDS010000119        10544 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEEDS010000119 DAEEDS010000000
VERSION     DAEEDS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.120"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(109..381)
                     /locus_tag="KLH32_003551"
                     /pseudo
     CDS             complement(109..381)
                     /locus_tag="KLH32_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(475..1037)
                     /locus_tag="KLH32_003552"
                     /pseudo
     CDS             complement(475..1037)
                     /locus_tag="KLH32_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1241..1447)
                     /locus_tag="KLH32_003553"
     CDS             complement(1241..1447)
                     /locus_tag="KLH32_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBE0140234.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1554..2117)
                     /locus_tag="KLH32_003554"
     CDS             complement(1554..2117)
                     /locus_tag="KLH32_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBE0140235.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3492..5327)
                     /locus_tag="KLH32_003555"
     CDS             complement(3492..5327)
                     /locus_tag="KLH32_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140236.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5428..5715
                     /locus_tag="KLH32_003556"
     CDS             5428..5715
                     /locus_tag="KLH32_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE0140237.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5687..7216
                     /locus_tag="KLH32_003557"
     CDS             5687..7216
                     /locus_tag="KLH32_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBE0140238.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7386..8603)
                     /locus_tag="KLH32_003558"
     CDS             complement(7386..8603)
                     /locus_tag="KLH32_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0140239.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8970..9956)
                     /locus_tag="KLH32_003559"
     CDS             complement(8970..9956)
                     /locus_tag="KLH32_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140240.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9953..10481)
                     /locus_tag="KLH32_003560"
                     /pseudo
     CDS             complement(9953..10481)
                     /locus_tag="KLH32_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3283 a         2417 c         1989 g         2855 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcact tacaccagac
      121 atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca cagcttccct
      181 actccagagg taatataaag gggtgggctc gactgattta tctttacgtc gcttacactg
      241 caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca gagaaggaat
      301 tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa ttttaggatg
      361 tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg cgttacagga
      421 aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa taggtcaaaa
      481 accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg tcaaataatc
      541 cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa tatccaggat
      601 gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt ccaaccctaa
      661 ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct catagtttcc
      721 cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca tttaaaggac
      781 tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg tttgccttgt
      841 tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg gaaacgagat
      901 atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc aatcacagga
      961 gggtaatgga gatccccccg tattgctgtt attctggaag tgtttttaat attcgtattg
     1021 atagttctca gagatatatc acactctaga tattttaatt aagtatcagt tcgatatcgg
     1081 catattgtac actacctcag gtagatgatg agatcagata cagggatcaa ctgacggtga
     1141 gcgcagtccc cacattttat ccagggctta ctgcatattc ccaacagcca ttcgttagcc
     1201 aggtagacga ataccctgat ttgccgctgg ttttgctttc ctatcgaagg gcccatacat
     1261 catcacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg gataatacga
     1321 aaacgcactg cacagggctc tgcggtttac gtcgccattc aatcccatga gcttcaagta
     1381 atgagaccag ccgggtattt tctgcccgca gtactgctag ttcatcgctg tcagatttat
     1441 aaaccatatg cgttatcact tgtacctgtc tcccggcggc accacgactg aaattcgtgt
     1501 gaaggcaaat ccacgcgata acttacgata gttcggacat aagcccccat aattcagtta
     1561 tgtcgtggtc gaccccggtt acttttttcg ggaagcctgc tacggcccag aataataccg
     1621 tgttctttct gatcctgttt agccagatag ctaagggcgt aacgtaagtc gtctaccgca
     1681 gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc tttagcccgg
     1741 cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc cagttgcctg
     1801 gatatctgat aagagttctt gtgacgttgt ccatccaggt agccgataaa atgaatatga
     1861 agccctttgt ctggtgtata ttccattacc cagtaatatc ccgccaacat cgtctgggtt
     1921 tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg cccgaaactg
     1981 ggcgtgtctt tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga aaaacgttga
     2041 aacataccat ccatgtgttc attcacgtct ttcagaatca tgaaatccat tttgtaattg
     2101 ggattggcat tatacatttt atagtcctta ctttataaaa ctcacaggaa gagagaatta
     2161 cgtaaatact gagagtagag aaacgccgta ccacggcatt aataaacgaa gtaaaccagc
     2221 agcacagaca acctgataaa actgttttgt attaccgcat tactgtgatg aggtaatact
     2281 tcagaataca gtgagactct cttaaactga gaaccagtac gaattacaat ggaaaaacac
     2341 acctcataga attgagtttc aggaagcacc actaaattct ttacaggaag taatctgaca
     2401 ttcacaaaac tcagtatcgg ctagctacag gcaacatcgc tattaatatt gtcgaaccat
     2461 taagtaaagc cgagagagac cacgggtatt taatcattag cataaaagaa taatggtata
     2521 cagaacatac ccacacacca taagaagatt gttccataca gaaaagatca tatttaccta
     2581 tacctataaa ctgtaacgga gtcataacac gctctaatag tgttccgata cgtaaccagt
     2641 gttaagtacg tacaggacaa cattacacac ataattcacc atacagcttc ccccatatgc
     2701 ttcactgcgt agtgtatgga gatatatgca ttacacacat acagaatact tagcataaag
     2761 aggagtgaca ttaccacgta aagagataag ttatacaaca aacatacaaa caatacggca
     2821 cagacacaca ataccataac tccaactaat aatactaatt aaccttaatt atttatggta
     2881 tatgaaataa atgtgattct cccggaacta actaacctaa ccagacagat gatcactgta
     2941 taaaaacttc agttaatact catttgtttt gtcgactcag tatataatga gtgaccagta
     3001 atcaggataa tcacacttac ataacctgta aaaaagaaca agaagaataa gccactaaca
     3061 taatgcaaaa tggcatctga atcgatcgca gtttggcatt cgaatcgatc atgatttggc
     3121 ccaatccgtg atcgaataga gcggaatacg caacataatt caccagacaa gagcttacac
     3181 cgaattacag attatgtatt aatgtcaaaa ctggcatatt ttacagatac aatgaacatg
     3241 caacccatca atctaatagg ggcggtatgt acatctgccc atgaatggga tggcgctaag
     3301 aatacaaaac aattagacat agatcacaaa aacacaccat tctaattgaa aaacaaaaca
     3361 aacaaataaa tcatactatt atagaatttt ctcttgccag acatagataa tacccctgct
     3421 aattttgcta atggttagca aaattagcag ggtatataaa gattattgac ccaaaacaca
     3481 ctcagatgaa ttcacgacag ctcaggaggg ttattcctga tgtcatcttc acaaataatg
     3541 gttaacgcat catatatcat tctgcgtctg ttcctgtttt cggccttatc gccatacaac
     3601 atcatcacat ttttaatttc atcgaccggt gatttcacat agtgaactaa tacaccaagc
     3661 ttctcaacac attgcagtaa agtatatttc ttactgcctg tgaaaagccc tatcatagtt
     3721 tctttacgtt cgcgactgga taatactttg ttttccataa tgacttctgc cagcgtctta
     3781 ccatcataaa catactcttt atctatcaga atatttatct tatccagcac cttctctacg
     3841 tcatcaagca aacctgtaat atatgggggc gcaatatatg gtttcgtact gatcttttca
     3901 agcatacaat tatacatctt aataacacgc atcagggtaa aattgttcgc atcatactgt
     3961 atttcaccct ccttatatac aggatcaata agtggtattt tttcaataaa tgcatcctga
     4021 taggctttta catggtcaag ccaataaaac aacgagccat actttatatt ttttctattt
     4081 actttaacct ccagtgctgt ccgaagcaac gataatgccc ccctgacata tcctgaaggt
     4141 aattcaccaa acaatccttt tctttccatg tcatcataat gctgcagcac atcattaatt
     4201 ttatttttcc ttatcagaca aatgtacacc gcaagtgttt cgtagcagct ggtcacatga
     4261 acagcctcat tctgcaactg cccaaaatag cttataatct ttttcagata tgcgtcatca
     4321 caaccattct tatcattaat attaaataca agatctttca gacgggaaat atattcctga
     4381 gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact gttttgaaac
     4441 ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct ggtaaagtca
     4501 taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc tctcaccaac
     4561 actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat atcaagtaag
     4621 acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac tttctccagt
     4681 tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt gatagccatg
     4741 atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt tcctgtatat
     4801 ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa tcccagttca
     4861 gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt atcagcgtga
     4921 tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata aaaagcagag
     4981 tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac cccaaaagtg
     5041 cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg ataagaattc
     5101 atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc ctgactgaac
     5161 accccttcag catcaataat acgactgatc tcattataac gttgatccca gaatttttga
     5221 tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccctc cttaaacaac
     5281 cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg ctgtgctctc
     5341 cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa actgacacaa
     5401 cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct ttacacgcct
     5461 tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag atctactatt
     5521 tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa accaataagg
     5581 cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct gatgttacat
     5641 acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga tgacgacaaa
     5701 actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg cattacttga
     5761 tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta tgctgacggt
     5821 catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc aacgtaccag
     5881 ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct ctgcctgcgc
     5941 aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat atatcgacga
     6001 taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa tcctggagcg
     6061 gagatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac gagcagcact
     6121 tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca tcgtgaatga
     6181 cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc tgttgctgtc
     6241 tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac tgtacaacac
     6301 attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt tccggctgaa
     6361 tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata agttttttac
     6421 tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat tttgcgaacc
     6481 cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg tgacgcaaca
     6541 atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact ctctgagagc
     6601 atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat caaatgcagc
     6661 atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac agaatcctca
     6721 tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca ggatagcagc
     6781 acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa gtactaatag
     6841 cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg aatcccttcg
     6901 ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta tcacaacagc
     6961 aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc tcaggaaaaa
     7021 atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa ggattgatga
     7081 taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa aaaaactaaa
     7141 tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac tgaagtatca
     7201 ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg tattattcgt
     7261 tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg aactggcatt
     7321 gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta tgccagtcca
     7381 tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata atccgcccat
     7441 gcctgcatca tcggtacacg ctgctccagt agatcagtac gatgatatgc cgcctcaacc
     7501 ttatttttca gcgtatgagc gagcgccctt tccgccagat cccgcgaata cccctgttca
     7561 ctacaccagt ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg ccccggaatg
     7621 tcactgacgg ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga aaggatctgc
     7681 tgctttctgg gtgaagggaa caccagttca tcatgcaggc cacgtatatt ttccagaatg
     7741 taaatagcct gccgggataa aggaacacga tgctgtagcc tagctttcat tctttctgca
     7801 ggtatagtcc atacccgctt atgaaaatca atttcagccc agcgcattcc cctggcttcg
     7861 cccgagcgag ttgctgtaag tatcaccatt aataacagtg cgcgggtaac attataaggt
     7921 tcatcactat acacactggt cgccacaaaa agcggtaact gcctccaggg cattgcgggt
     7981 tggtgttcat cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac atcaacagga
     8041 tttgccacac aaaaaccgtg cgcccatccc cactgcataa caacatgaat gcgctgttta
     8101 acccggcttg ccgtttctga caaggttaac cagactggac gcagtgtttc tgccacgtcc
     8161 gcagccgtaa tcgaatccag cgtttttgct cccagttgag gaaacgcgta attctcaagc
     8221 gtcgataacc actgccttac atgctttgga ttctcccatc caggagacag ttctgcatgt
     8281 acacgcctgg ctgcatcggc aaatgttggg ataacgactt tctcagattc agccttttta
     8341 atctccagag gatcatcacc tgcagcaagt tgctctcgca ttatccgggc agtacgtgca
     8401 gcttcagcaa tactgacctc tgggtaagtt cccaatccag cattacgtct tttttgtgtc
     8461 accggactta cataacgaaa aacccatttc ccccgcccct ttactgaaga aggatgaagg
     8521 gtcagtccgg taattccccc atggggcaat ggtttgtcat caggtttgat atgtcttgct
     8581 ttcgtatccg tcaatactgc catacgctaa ttcccgtcac tctggtatgc catccagtat
     8641 gccattagtc cctcgcttca ctaagaatac attagataac atcggacaac aaaaagtgta
     8701 atacattgtt tataaagaaa ttatataaca cccctggatt aagtcagatt tatttcaggc
     8761 ggtccccctc accgccatat ttaaagaaga gcgcgtacga aagtacgggc ttttttttcg
     8821 tatattgtac acaccgggtg cccccgccca cgtctgttag ggcgagggga aattgtgcac
     8881 atcaactgtc tggttactca gtcaacaatc aaaatgcgta agatagtaca gaacatatat
     8941 gcgttatctg gtcacttcct gtaacttcct cacttattaa aatgccccca acggaaatac
     9001 atgtcacatg ccatcataaa atcgttatgc cagaggaatc gacaatggaa aaaatagtaa
     9061 attaagcacg gtacatagca gagtaaaaaa taggcttcaa accaaatccg ccattccaca
     9121 agcgttgcca ataccgacac cagcgtaatc cacatcattt caataacccg caatatgaat
     9181 aaccgaagcc aaagcggctt ggcaaaaaag gtcgacaacg tattttcact ggagaaaaac
     9241 agatcgattt cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga gaagcgcaga
     9301 aaaaacagat taatcgccaa cactacgcca caaagaatga gcaatgcaat tatcgattta
     9361 aacgttaacc actgcccctc tgtcacaaaa gaatcactgt tacgcaacgg gctgatagta
     9421 agcaggttaa caaccgttga gatgcacacc gaaagcagca catagcgagt cagactttta
     9481 tattttactt ttttcgctac tgaaatagcg acctgcttga cataactggt gcggatcatc
     9541 cgaaacagca gaaagatgac caacggccca agtaacgaaa tcaggaacag cgcatataac
     9601 gagggtagaa gctggtatag cccccaactc attgcactca acatgattaa cgacagaaat
     9661 cctgccacca gaggttgtgt aagaaataat tctattttgt ggttctgctt tttcttaaac
     9721 cacgaaaaat cgatattctt cgtgacgcat ttataagccc agtacgcctt gagatcaaaa
     9781 ataaaattat acagcaccag ggccagaatc ccactaacta caccaatatc ctctgatgga
     9841 aaagtaaacc ctgcattttt ccaataaaag cctatcagaa taagtccaat agcataaatg
     9901 tatatcagat ttcgtttatc ctgaaagcga ataaaagtta aataatcagg aatcatttga
     9961 atacctttgc atcactaaat gctttacgac aatgcataac tcgttgctga tccacgccag
    10021 caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg aaagctaccg
    10081 gggttggcat attcagaata tgtatcgaga gattcagtcc atttcaccgt atgagttaag
    10141 tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc tgttacgttc
    10201 atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc gcgcattgcc
    10261 tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag tttaaccggt
    10321 tcgtatccag cggccatcag cagcgtggta aattcattca acgcagcctg cgttccccca
    10381 ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc tgattccgta
    10441 atcatcgtct gcagttgttc gcggttgatg cctttaaccg cctgatctta cccagcaata
    10501 gtggacacgc ggctaagtga gtaaactctc agtcagaggt gact
//
LOCUS       DAEEDS010000120        10125 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEEDS010000120 DAEEDS010000000
VERSION     DAEEDS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.121"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="KLH32_003561"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="KLH32_003561"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBE0140241.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KLH32_003562"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KLH32_003562"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE0140242.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KLH32_003563"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KLH32_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBE0140243.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="KLH32_003564"
     CDS             complement(4692..6452)
                     /locus_tag="KLH32_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBE0140244.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="KLH32_003565"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="KLH32_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="KLH32_003566"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="KLH32_003566"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBE0140245.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="KLH32_003567"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="KLH32_003567"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBE0140246.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            9975..>10125
                     /locus_tag="KLH32_003568"
     CDS             9975..>10125
                     /locus_tag="KLH32_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBE0140247.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLAPELISRCLAE
                     SGTVTL"
BASE COUNT         2565 a         2428 c         2531 g         2601 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacggc gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaat cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatatgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattgtcgta atgccgatca gttaaggatc agttgaccga tccagtggct gtgtaagaat
     9961 ccggaaacgc tcacttgttt ccggattttt ttatgcacat tggacaggct cttgatctgg
    10021 tatcccgtta cgattctctg cgtaacccac tgacttctct gggggattac ctcgcccccg
    10081 aactcatctc tcgttgcctt gccgaatcag gtactgtaac gctac
//
LOCUS       DAEEDS010000121        10090 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEEDS010000121 DAEEDS010000000
VERSION     DAEEDS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.122"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..177)
                     /locus_tag="KLH32_003569"
     CDS             complement(<1..177)
                     /locus_tag="KLH32_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE0140248.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
     gene            complement(380..847)
                     /locus_tag="KLH32_003570"
     CDS             complement(380..847)
                     /locus_tag="KLH32_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0140249.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(844..1377)
                     /locus_tag="KLH32_003571"
     CDS             complement(844..1377)
                     /locus_tag="KLH32_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBE0140250.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1443..2072)
                     /locus_tag="KLH32_003572"
     CDS             complement(1443..2072)
                     /locus_tag="KLH32_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140251.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2076..2291)
                     /locus_tag="KLH32_003573"
     CDS             complement(2076..2291)
                     /locus_tag="KLH32_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0140252.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2584..2658)
                     /locus_tag="KLH32_003574"
     tRNA            complement(2584..2658)
                     /locus_tag="KLH32_003574"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2623..2625),aa:Gly,seq:tcc)
     gene            complement(2662..2737)
                     /locus_tag="KLH32_003575"
     tRNA            complement(2662..2737)
                     /locus_tag="KLH32_003575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2702..2704),aa:Thr,seq:tgt)
     gene            complement(2740..2816)
                     /locus_tag="KLH32_003576"
     tRNA            complement(2740..2816)
                     /locus_tag="KLH32_003576"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2780..2782),aa:Arg,seq:tct)
     gene            complement(2818..2894)
                     /locus_tag="KLH32_003577"
     tRNA            complement(2818..2894)
                     /locus_tag="KLH32_003577"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2858..2860),aa:Ile,seq:cat)
     gene            complement(3094..3474)
                     /locus_tag="KLH32_003578"
     CDS             complement(3094..3474)
                     /locus_tag="KLH32_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0140253.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3467..3667)
                     /locus_tag="KLH32_003579"
     CDS             complement(3467..3667)
                     /locus_tag="KLH32_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBE0140254.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3762..4019)
                     /locus_tag="KLH32_003580"
     CDS             complement(3762..4019)
                     /locus_tag="KLH32_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140255.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(4016..5407)
                     /locus_tag="KLH32_003581"
     CDS             complement(4016..5407)
                     /locus_tag="KLH32_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE0140256.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(5404..6282)
                     /locus_tag="KLH32_003582"
     CDS             complement(5404..6282)
                     /locus_tag="KLH32_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0140257.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(6293..7201)
                     /locus_tag="KLH32_003583"
     CDS             complement(6293..7201)
                     /locus_tag="KLH32_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0140258.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7188..7421)
                     /locus_tag="KLH32_003584"
     CDS             complement(7188..7421)
                     /locus_tag="KLH32_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140259.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7418..8434)
                     /locus_tag="KLH32_003585"
                     /pseudo
     CDS             complement(7418..8434)
                     /locus_tag="KLH32_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            8366..8629
                     /locus_tag="KLH32_003586"
     CDS             8366..8629
                     /locus_tag="KLH32_003586"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0140260.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8619..8927
                     /locus_tag="KLH32_003587"
     CDS             8619..8927
                     /locus_tag="KLH32_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0140261.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8969..9145)
                     /locus_tag="KLH32_003588"
     CDS             complement(8969..9145)
                     /locus_tag="KLH32_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBE0140262.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(9142..9552)
                     /locus_tag="KLH32_003589"
     CDS             complement(9142..9552)
                     /locus_tag="KLH32_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBE0140263.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(9549..9827)
                     /locus_tag="KLH32_003590"
     CDS             complement(9549..9827)
                     /locus_tag="KLH32_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140264.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(9902..>10090)
                     /locus_tag="KLH32_003591"
     CDS             complement(9902..>10090)
                     /locus_tag="KLH32_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140265.1"
                     /translation="ASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIAN
                     MPKSEYDEAWRQENVTEY"
BASE COUNT         2440 a         2707 c         2314 g         2629 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgtgatttg caaaaaaatt tatttcaggc actgagtcct gatgtactcc tgaagcattc
      421 tcaatgctgt ctggtcgctg atgattccgt cccggatacc gagaacgttt cgtccagcaa
      481 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
      541 aggatggcac agggcatttt cctttgacga gcacccgacc accattatca agcttgcgcc
      601 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
      661 catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc agcttcagtt
      721 ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg taatgattaa
      781 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
      841 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
      901 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
      961 aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg
     1021 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
     1081 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
     1141 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
     1201 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
     1261 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
     1321 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
     1381 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
     1441 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
     1501 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
     1561 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
     1621 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
     1681 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
     1741 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
     1801 tcctctccgg caccgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     1861 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     1921 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     1981 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     2041 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     2101 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     2161 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     2221 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     2281 atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     2341 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     2401 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     2461 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaag gcgatcattc
     2521 ataacttaaa cgtctttcag tcactccggg atttcccatc atcgcagact gaaagactct
     2581 gactggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag
     2641 ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg acctactgat
     2701 tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg
     2761 aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg
     2821 tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc tctgaccaac
     2881 tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct
     2941 gaccaccagc gtgtttaacg tcctgtaccg tttttcaggc ataaaaaacc cgcccggagg
     3001 cgggtttaag ctgtgtggcg aagcaaccac tcttaacaga atatcccgtt ttttacgtac
     3061 gtaaaatatt ttctagaaag tcgcccctta ccatcaggga tgttcaatat atttgtccat
     3121 ttctaaccgg acacccaaca tcatcaacat tccttccacc accccctcgg ctttttgtag
     3181 cttctttcct atataaccat ctgagcatcc atgcttccgc gccagcgtca tgaatgtcat
     3241 cccaaacacg tagtagtcaa ccagcaagtc atgtagatcg ttgttgttcc ggttaaggcg
     3301 ggccatacac ccacatataa ccatcgcgtc atcgtcacag cattgcgggc gggattttac
     3361 ttttgaaggg attaatccct taaaaccggc agcaatggac gaccaggtca catcctcatg
     3421 attattagcc acccacgcgc cccagcgctc aagaacctgc tgaatatcac gcatcactgt
     3481 ctttacccct gtcccatcca cgatgaacaa tcagaacacc atcaacaatg gcgtgccctt
     3541 tgccctcttt atcttcagca tattttctta ccgtggcgcg attacagttc agcattcgtg
     3601 aaacttcggt catattacct cgtgtctgga taagcagttc cggtatcgtt tgaattttga
     3661 cgctcatcaa atactctccg gttcggtgat ttttatcccg ggctttccac cagggacata
     3721 atgatcgcgc acaagccagg gcaaaacaaa ctcatggcgc atcagcgcag cgcctcctgc
     3781 accagttttt caaactttcc gactctggtt tccagctctg ccacacagtc caccagctca
     3841 tctactgctt tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc gggtaccata
     3901 tctttacgga tagcatcttt tgttatgccc gttttttcga gttgttcagc atgacgcagc
     3961 atttcctgcg cgtgtttacg caattgttca ggggtaaaag tcattgtctg gttgttcaaa
     4021 agaaacgctc catcttactg ctgtcagttc gtttattact gtatctgcgc ggattgccgg
     4081 gcttcatggg agtggaaagc acccgtgcac tttcctggtc cacaggcaga aaatgtccgt
     4141 tataaaaacg ccggtaaatc gttcccagag aaccgttacg ttgtttcgtg atattgattt
     4201 ctgcgatgcc cctggcctgc gtatccgggt tgtacacttc atccctgtaa agcatcagaa
     4261 tgatgtctgc atccgcctct atttctccgg aatttttcag gtctgagttc atgggacgtt
     4321 tattgggtct ggactccaca ccgcgggaga gctggctcag cgcaatcaac ggaaaaccac
     4381 cggattttgc caggccttta agcccctttg agatttcacc cacggcaagg tcatgacgcc
     4441 ccgtggttcg ggtttttatc agcccgagat aatcaaccac caccagcgcc gtttccggat
     4501 atttaatcag gtggtgtttc gttgttgcgc atatctcatc aatggccagg ttcgcctggt
     4561 ccaccatcca gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa cgcgtccagt
     4621 cttcatcttc aaagtgagcc acagatttca ggcgtgatac tggcatccct ccagccgcag
     4681 acaccatacg ttcaccaatc tggatgttcg ccatctccat ggtgaacaga agcacaccat
     4741 gcccctgctc agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc cccatcgaag
     4801 gacgggcggc aatgaatacc aggtctccgg gctccatacc gcctgttttt gcgtccagtt
     4861 catcaatacc ggtcatcaac gtcctggatt tctccagccc ctgattccgg cattcaacac
     4921 gctcaaccac ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg ccctttcctg
     4981 tcgacagtgt gaccatcatg ttctgcgcat ccttcagggc atcttcagct gcttcacagg
     5041 tatgcgcatc acgtaatttc tgcagcgcct cattcagtgt tttttctgca tcgcgcagtg
     5101 cggcattgcg ccgcaacgct gcaacatagt gctccagtga agacttcacc caggttttac
     5161 gcccggtatc agtaatcacc ggggcaagtt ccggcatctc attacacaac agcacggggt
     5221 caatcactcc tgaaacacgg gcctgtctgc agatgcctgt atagatatcc tgatacgctc
     5281 gtacagaaaa aacgtccgct ggcagtgtgg ccagaatatc catcacttca ggatctgctc
     5341 cgcgcagaaa gaacgcgccg atgacagcgc cttcaaggtc atcgttacgc catactggag
     5401 ttgtcatgca gccacacctc tgatacgaga acggtaactg ggccagttaa acgacaacca
     5461 gttgcgtccc ccgtctgtga tcctgtcggc aattcgggga ctgatgaacg cccacaactc
     5521 ttccggtgag aggttactga taagaatggt gggcaagata ctttcgtacc tggcgttgat
     5581 aatttcctgc aaaatagcca tttcagccgc gctgccaaac tgaacgccaa cttcgtcgat
     5641 gatcagcaaa tccattgacg cataacgctc aataacttca tccgctgttt tttcgctgtc
     5701 attccgccag caatttttca cagcacgggt aaggcgcatc acgtcggtga tctccacact
     5761 ggccagatag ttacggatga tgtgttttgc catagccaca gccagatgat tttttccggt
     5821 accacaactg ccggtcataa caagactggt accgttctcc agcatatctg gccagttctc
     5881 cgcatagcgg cgacaggccg caagatttct ggctgcgcca ggattaacct ccagataatt
     5941 atcaaactcg cagtcccgaa aacgcagggc aattccggcg ttatcagtca gctcttccgc
     6001 cttgatggac gacagctcca tggtcaaatc gttggcctca gcgattaagc agtcagggca
     6061 gcatgaaatt tcttctctgt cctcgccatt acgatcgctc cacaccagta tatgcgtgtg
     6121 atattcgcca tgtttttcgc aatatccgcg accttcacgc atcaggcagg aacgataagg
     6181 ccatggcttt tcgcccttct gagcaaatgc aatctctgcc cgtaactcat ccattcgcgc
     6241 ctgtagtctt ttttgttgtt cacgcaggtt aaacgtcatc atcgctgtca cctcagaatg
     6301 tcagtttgtc actggattta ccgaatttgt cagacatggc tcccaggcca gccaggacat
     6361 cgacctgtcg ctgtcgccca cctctgggag cggctggctg ttgccagaag tcttcgaagt
     6421 gacgatcggg tccaaagaac gtcgcagcct gcttcacgaa ctgtgtgccg gtatttcctg
     6481 tggcacgtac ccaggcagca tagcgtttca cgccatcaag catggcttca ggttttattc
     6541 cctccctgag ccgggctttc caggctttga aggctgcgga ttttgagtta ccaccagcac
     6601 gttttggata ttcctgccag gcctgttcaa attccggtga atattcctgt cgggaagaac
     6661 gcgctggtgc agacgcgtca gcggatgcgc caatagtgtt tttactctct gtagtattct
     6721 ctgaagtaat ctctgttgta ttctctgtaa gatcgaaatt ggttttccct tctccgcggc
     6781 gaggggtttc ccgtgtccgc ggtgaaggct ttccctcctc cgcgaaattg ggttttacag
     6841 tttcccgaaa acgggtttcc ccatttcggg aaaactgatt gttttcattg ataatttcat
     6901 taaggcgctc acaatctata cggtagaaca ttttgtgctc aagacgcttg ttggtttcaa
     6961 ccaaaatgcc tctggacaca agatgcttac gcgctacagc ctgttgttca aatgtaagtc
     7021 cggtttcgtg ttgtatctct tcacgcgttt tatgtacgcc ttccgctgca tgtgctttat
     7081 cctgccagta aaaaatctga ccaaagaaaa taacagcgtg cggacttccc atgtatttaa
     7141 cgagcccagg gtaataagca accggatgtc caaaatcgag cagaagatca gacggacgca
     7201 tagccacctc ccaggcgttt aaacattttt ccgggctgaa acgccaccag cggataactc
     7261 agggtatgag tacgtccctg aacctggcag acaaccttct ggctttcggt actgaccagg
     7321 caaacccgca gaacgtagcc gttgctggtg gtgaaccact gccccacacg ggggcaatgg
     7381 ttgtatcggt gatacaggaa attaacgatg tggcggatca tgggcgcacc tccttgtcag
     7441 aaccattcag cctggaatca acaagtgcag caccaaaaac agcatcacct acacggtcgt
     7501 acagtttgcc agccagcgga gattcaacgg ccttaagcat tgggtaaagc tggcttgtcc
     7561 agatttgatg gatttcacgc aaatgcaggt atacgcctct ggcgttttgt gcgacagatg
     7621 acatgtcagc cgcaccagct cctgataagc tcctctccat ctggttaaag gcattgatgt
     7681 atgcctcttt gaaccgggcg gcacgtttac cagtgaagcc catagcaagg aaggcgaagc
     7741 cgccgcgggt aatttggtag cagggaagtt tgcgagtacc gccgttgggc tggcgtacca
     7801 aaattgatgt ctccgcaaaa ttgcgggcac aaaactctgg agaacaatcc aaaatgcgga
     7861 tctttttcag aacatcgtca tgacgtttag agaagaagtt ggcaacagcc agggatgaag
     7921 taacagccta accatcaacg atggcaattt caggttgaga gagggttggg agagtagtca
     7981 tggtgacagc ccctatgttg aattcaaaga actcaccaca tgggacgcca atcacagagg
     8041 tggtgagacg tacagggttg gcgtaaccgg tcaacataga acccggcgca tcttgcgatg
     8101 tccctgcacg ccccaccata atttgggcgt agcaatgctc atgacacgaa aaaaccgcat
     8161 gagcgcggtt atgctctata ttgaatttca ggacgccaat cccggccccc gctttataag
     8221 gtgcagagac agtgtaacgt cccgaaattg cagaatcaat atattgtgca gatataacga
     8281 ttttgttatg ttttgctgta tgaactacac tatcgaatac tacagtgaag aggtccgact
     8341 ggaggtcgat cagcttccat tgagtatgcg tgccagatac cagcatcttg ttgaacgcat
     8401 gaaggtatac ggcagcaatc tcggagaacc tcacaccagc gcctttggtg acgggctttt
     8461 cgaactcaga attaaaggca acgatgggat cgcgcgtgtt ttttactgca ctctgacagg
     8521 gaaacgcatc atcatgctgc atagttttgt aaagaaagcg cagaaaacac cgccagccga
     8581 acgcaagaaa gctgaaacca gaatgaagga ggttaagcat gactggtaaa cgcactctcc
     8641 ccaccatgac acacgacgaa atggcagcca aatggatgga agacccggct tttaaagcag
     8701 aatacgacgc tatcgctgac gaattcgcac tgcttgatga aatgctggca gcacgcaaag
     8761 aagctggctt aactcaggct gaagttgccg agcgaatggg aacaaaagcg accgcgatca
     8821 ccagaatgga aagtaatctc gcatcaggta tcagcggccc atcatttgcc acactaaaaa
     8881 aattcgcccg cgctactgga aaaaaactcc agatccgctt cgtttaacca caccgcgccg
     8941 tcattctggc ggcgcaccgg ataaatagtc atttcctcgc acgatgtctt agccaccgga
     9001 tatcccacag gtgagtcgtg tagttgaagg tttttacgtc agattctttt gggattggct
     9061 tgcgtttatt tctggagcgt ttcgttggaa ggtatttgca gttttcgcag atgatgtcgg
     9121 tgaaacttcg tcgatgtcgc ctcattcata cctcctgtcg gtaaatctga caccctgacc
     9181 aatagcccag gctgttgtgt actcgatcag acttgccatg cgcttcacgc tcatctgcgc
     9241 gctgctttcg cgaatgttga cgcattctcc ttcaaggccg ggcaaaacat cagcttcctg
     9301 ttttgttgcc actgcatgac cgctgatcaa caaaaccttc cattgttctg gttttaacca
     9361 tttgccgcac cattgaacct gacgagcgat atccgccagc atcgcgtgaa attttgcgtt
     9421 ctggtcaaga tttcgcttgt agtcagtaat gcggatggtg actggcttgt ctttatcgag
     9481 aggagttgag aggatggcat ttattgcggc ttgctgttgt tgcttagttc ggaggaagat
     9541 tgtttgcttc atcgaaaatt cttctcttta attccagcgg ctctgatagc tttcattact
     9601 gcaattaccg ttttgtcctt cccatcctca tgccccatcg cataagcacc ttcttcacca
     9661 tctttccaaa agtcgtcatt cgattcgggc cagtcgatat ccagttcaat agctgctcgc
     9721 gatgcctgcc atatcaccca ggcaaactct tttaattcat cgtctcctgt gaactggctt
     9781 ttgtcttttg accaccagtt ttcaaactgt cggtagctat cgttcacttc cctctccccc
     9841 aaataaaaag gcctgcgatt accagcaggc ctgctattag ctcagtgatg tagatggtca
     9901 tttaatactc cgtcacgttt tcctgtcgcc acgcctcgtc atattccgat ttcggcatat
     9961 tggcgatgta gctatatggc gatcctgatt caagttgcag gaactggtgc gattgctcgt
    10021 caaggaacaa cggaacacca ccttcccaac cttcgccgtt acgttgtttt tcaagcatca
    10081 aaacagatgc
//
LOCUS       DAEEDS010000122         9953 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEEDS010000122 DAEEDS010000000
VERSION     DAEEDS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.123"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="KLH32_003592"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="KLH32_003592"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBE0140266.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="KLH32_003593"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="KLH32_003593"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBE0140267.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="KLH32_003594"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="KLH32_003594"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBE0140268.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="KLH32_003595"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="KLH32_003595"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBE0140269.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="KLH32_003596"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="KLH32_003596"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBE0140270.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="KLH32_003597"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="KLH32_003597"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBE0140271.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="KLH32_003598"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="KLH32_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="KLH32_003599"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="KLH32_003599"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBE0140272.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="KLH32_003600"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="KLH32_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBE0140273.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9953)
                     /locus_tag="KLH32_003601"
     CDS             complement(8974..>9953)
                     /locus_tag="KLH32_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0140274.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2350 a         2677 c         2507 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa tga
//
LOCUS       DAEEDS010000123         9929 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEEDS010000123 DAEEDS010000000
VERSION     DAEEDS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.124"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..338
                     /locus_tag="KLH32_003602"
     CDS             <1..338
                     /locus_tag="KLH32_003602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140275.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            526..1641
                     /locus_tag="KLH32_003603"
     CDS             526..1641
                     /locus_tag="KLH32_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140276.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2320..2538
                     /locus_tag="KLH32_003604"
     CDS             2320..2538
                     /locus_tag="KLH32_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBE0140277.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="KLH32_003605"
     CDS             2756..2998
                     /locus_tag="KLH32_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140278.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KLH32_003606"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KLH32_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBE0140279.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KLH32_003607"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KLH32_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE0140280.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KLH32_003608"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KLH32_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBE0140281.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KLH32_003609"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KLH32_003609"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE0140282.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="KLH32_003610"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="KLH32_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBE0140283.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2491 a         2396 c         2542 g         2500 t
ORIGIN      
        1 tgtcgagtaa taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca
       61 aagtaccaga aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtaatc
      121 gcttactcaa cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg
      181 tttatggcaa agatatggtg acaaagtccg gggatatatt gattgatata caattaaata
      241 aggatggcag tctccagagt aagcactaca cacttgatgt aggtgaagtt ataaacagtt
      301 ataatattga ccctctttgt gaatgcttca agtattaatc aaacttactt acatgataaa
      361 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
      421 gaatgcacgg gtaaaaatca tcaaattaat gttaaagcca aattgccaca agaacgaagt
      481 gaaaaagttg aggatatgtg tgaaaaaatg aaaggtggca tctctatgtt taaccacacc
      541 acaaaaacag ctgataatat agagcataat ttgcagctaa gttttctcgt gggttcacgt
      601 ccgtcgatca ccaataaata ctcagcaaaa gatgtaggtc cagatatact tgcattgccc
      661 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaata atgcagtgga gaaggcttca
      721 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg
      781 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa ttatttttga tttgcgtttt
      841 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggcgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacaagccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     9901 tattaaattt caggacttat tcatttcgt
//
LOCUS       DAEEDS010000124         9783 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEEDS010000124 DAEEDS010000000
VERSION     DAEEDS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.125"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..143
                     /locus_tag="KLH32_003611"
     CDS             <1..143
                     /locus_tag="KLH32_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140284.1"
                     /translation="AEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            complement(194..514)
                     /gene="psiF"
                     /locus_tag="KLH32_003612"
     CDS             complement(194..514)
                     /gene="psiF"
                     /locus_tag="KLH32_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBE0140285.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(633..2048)
                     /gene="phoA"
                     /locus_tag="KLH32_003613"
     CDS             complement(633..2048)
                     /gene="phoA"
                     /locus_tag="KLH32_003613"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBE0140286.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="KLH32_003614"
     CDS             complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="KLH32_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBE0140287.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2591..2794)
                     /locus_tag="KLH32_003615"
     CDS             complement(2591..2794)
                     /locus_tag="KLH32_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140288.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2872..3966
                     /gene="ddlA"
                     /locus_tag="KLH32_003616"
     CDS             2872..3966
                     /gene="ddlA"
                     /locus_tag="KLH32_003616"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE0140289.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3990..4202)
                     /locus_tag="KLH32_003617"
     CDS             complement(3990..4202)
                     /locus_tag="KLH32_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBE0140290.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4462..4770
                     /locus_tag="KLH32_003618"
     CDS             4462..4770
                     /locus_tag="KLH32_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBE0140291.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="KLH32_003619"
     CDS             complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="KLH32_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBE0140292.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="KLH32_003620"
     CDS             complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="KLH32_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBE0140293.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7508..8665
                     /gene="ampH"
                     /locus_tag="KLH32_003621"
     CDS             7508..8665
                     /gene="ampH"
                     /locus_tag="KLH32_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBE0140294.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8666..9169)
                     /locus_tag="KLH32_003622"
     CDS             complement(8666..9169)
                     /locus_tag="KLH32_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0140295.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9257..>9783)
                     /locus_tag="KLH32_003623"
     CDS             complement(9257..>9783)
                     /locus_tag="KLH32_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0140296.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2437 a         2493 c         2333 g         2520 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 tggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcaatcag gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat
      241 cacctttcag agcttgttgc gttgcttgat tattacattc acgcatcttt tgctgctgtg
      301 gcgtcaaact tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg
      361 tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacag gaggtcatac
      421 gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac
      481 caaaaagcaa ggtaaccagt aatgttattt tcatagcacc atccctcttc atgttttaac
      541 catgagcgtt tgcgcccgtg atctgccatt aagtctggtt gctaacagca aaaaaaccac
      601 ccggcagcga aaattcactg ccgggcgcag ttttatttca gccccagagc ggctttcatg
      661 gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac
      721 gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga attcccgtaa
      781 ctcatcacca tcactgcgcc atctttagta tttagcgcct gagtgaggcc cggcgctttg
      841 gtatccggcg caacaacctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg
      901 ttaccctcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
      961 tcgccaattt gcccacaagg gttcgcagcg tgatcctgtt tatcgattga cgcaccttca
     1021 acttgcagga aaaatccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
     1081 tgtgccaggg ttggtacact gtcattacgt tgcggattag gcgtataggt gactgcgggc
     1141 ttatcgatat tgccgtggta cgttgctttc ggccctagcc agcgcactgg catattgccg
     1201 tcagcaaaca ggccaagcag gggtttttgc tgattcgctt ccgtcaccga attcagtgag
     1261 gcagcatcgt tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
     1321 tgccattcac cagcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaacg
     1381 tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
     1441 ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga agtcacatgt
     1501 gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
     1561 agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
     1621 cccagcgcgc cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag
     1681 tcggtgacgt agtccggttt gccagttttt ttattcagcg catagtgagt gtattgcccg
     1741 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt
     1801 gcggcagtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca
     1861 ggtttatcgc taagagaatc acgcagagcg gcggtctgat cacccgttaa gcggcgagca
     1921 ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
     1981 gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt
     2041 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
     2101 agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacaagg
     2161 atgcttcaat aacttcttta cgtaatcgcg cagcagctct gtatcgtcgt caggaatgct
     2221 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc gctgttggtc
     2281 attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc
     2341 acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
     2401 atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtttcacaa
     2461 cttcatagtg tctggaagta ttatttttat gacaaaggaa atactttgcg caaatatttt
     2521 ttgtttgatt accagcgaaa cgtttctctt atttaaataa ttacccacac aaaatatagg
     2581 cttttaatta tcaaccttta ttataaataa aagctatgaa atatattctc ttcgttaact
     2641 tataattaag agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc
     2701 cgttcatcat ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta
     2761 cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg
     2821 ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa
     2881 ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg
     2941 gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt
     3001 gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat
     3061 cctgcccata ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat
     3121 gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc
     3181 ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc
     3241 aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc
     3301 accaaacgtc tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc
     3361 gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt
     3421 gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag
     3481 tacgcaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg
     3541 atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc
     3601 tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa
     3661 gatggcgcga aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg
     3721 gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg
     3781 tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc
     3841 aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc
     3901 acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca
     3961 atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga
     4021 cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca
     4081 taggtctgcc aaccttttgc ttcaaatccc agtaccccca ccacgccatt aagaatgaat
     4141 ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc
     4201 atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt
     4261 gtagacaaca ttttcgtaat tattaataaa tttaacttaa ggttgccatt ttgtgactca
     4321 caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg
     4381 acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc
     4441 agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga
     4501 aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt
     4561 tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag
     4621 cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc
     4681 accattttcg ggctgatccc gtttttagca ggctgcctca tttttgcagt ggtggcgcta
     4741 tggctgcact ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg gcctgaaacg
     4801 ccttatctgg cctgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt
     4861 ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt
     4921 gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat
     4981 aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac
     5041 ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg
     5101 ttttcctggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc
     5161 cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt
     5221 taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt
     5281 aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca
     5341 ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc
     5401 caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga
     5461 tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc
     5521 tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa
     5581 tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa
     5641 cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc gagtagacgt
     5701 tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg
     5761 catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg
     5821 ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa
     5881 ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc
     5941 tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca
     6001 gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga
     6061 aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg
     6121 taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat
     6181 agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc
     6241 gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac
     6301 cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg
     6361 gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg
     6421 caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga
     6481 gcgttaccag caccggcagg aaggcgatca acgtcattat ggcgttgata aaactgacgc
     6541 ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac
     6601 cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc
     6661 agcggaacac gtagtgactg acaaagaagt tgttcagtac actgatcacc actgcgatca
     6721 gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg
     6781 gcgaacttag cgcggtttga atcagatcat agaacggcgc ataccaggcg ttgacggcga
     6841 ccccgacttc caccaaaaac caagtgacga agataatcag cgcagtaccg agtattgacc
     6901 agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa
     6961 caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg
     7021 ggatctgccc ggaagcgccg gtgatatgtg ccacccagtc acccccaccg gcttgccaga
     7081 agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga
     7141 aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta
     7201 ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc
     7261 aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa
     7321 gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag
     7381 gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc
     7441 tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac
     7501 accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc
     7561 cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta
     7621 tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg
     7681 taaccagcgc gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca
     7741 gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt
     7801 gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc
     7861 aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca
     7921 taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg
     7981 gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg
     8041 cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc
     8101 gtccggcaag ccttataccc agttgtttga agagcagatt acccatccat tggggatgaa
     8161 agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg
     8221 tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc
     8281 tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa
     8341 ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg
     8401 catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa
     8461 agagggtcgt ccgaggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat
     8521 ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac
     8581 gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa
     8641 accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca
     8701 gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga
     8761 cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc
     8821 gcaattcttc attccagtca atcattgata ataatgttgc gcgggtttgt ttggctggta
     8881 gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta
     8941 aaccatcagc cagccccata atataaggag cctgggtaat accgataagt acgttttctt
     9001 ctctacgcag agagataact ccttctaaaa taacgaaagt atcttcgtta ttgacatcac
     9061 tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac
     9121 atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct
     9181 ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc
     9241 ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac cagtcttgat
     9301 ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc
     9361 tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat
     9421 caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg
     9481 gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat
     9541 aacgcatgct gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat
     9601 caccagactg gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca
     9661 gtttgaagtc gtaaccggtt ttcaaaccga agccccaagc gtcggagttg gtgctaccgt
     9721 caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca
     9781 agc
//
LOCUS       DAEEDS010000125         9644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEEDS010000125 DAEEDS010000000
VERSION     DAEEDS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.127"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KLH32_003624"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KLH32_003624"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBE0140297.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="KLH32_003625"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="KLH32_003625"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBE0140298.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="KLH32_003626"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="KLH32_003626"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBE0140299.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="KLH32_003627"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="KLH32_003627"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBE0140300.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="KLH32_003628"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="KLH32_003628"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBE0140301.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="KLH32_003629"
     CDS             5060..6193
                     /locus_tag="KLH32_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE0140302.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="KLH32_003630"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="KLH32_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE0140303.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KLH32_003631"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KLH32_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBE0140304.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KLH32_003632"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KLH32_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBE0140305.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KLH32_003633"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KLH32_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBE0140306.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KLH32_003634"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KLH32_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE0140307.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="KLH32_003635"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="KLH32_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBE0140308.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2473 c         2459 g         2261 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tctt
//
LOCUS       DAEEDS010000126         9625 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEEDS010000126 DAEEDS010000000
VERSION     DAEEDS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.128"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KLH32_003636"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KLH32_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBE0140309.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KLH32_003637"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KLH32_003637"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBE0140310.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="KLH32_003638"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="KLH32_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KLH32_003639"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KLH32_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBE0140311.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KLH32_003640"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KLH32_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBE0140312.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="KLH32_003641"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="KLH32_003641"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBE0140313.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="KLH32_003642"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="KLH32_003642"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBE0140314.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="KLH32_003643"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="KLH32_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="KLH32_003644"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="KLH32_003644"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBE0140315.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2269 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaat
//
LOCUS       DAEEDS010000127         9493 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEEDS010000127 DAEEDS010000000
VERSION     DAEEDS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.129"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..190)
                     /locus_tag="KLH32_003645"
     CDS             complement(<1..190)
                     /locus_tag="KLH32_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBE0140316.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="KLH32_003646"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="KLH32_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBE0140317.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="KLH32_003647"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="KLH32_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBE0140318.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="KLH32_003648"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="KLH32_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBE0140319.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="KLH32_003649"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="KLH32_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="KLH32_003650"
     CDS             complement(3262..3381)
                     /locus_tag="KLH32_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBE0140320.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="KLH32_003651"
     CDS             complement(3342..3527)
                     /locus_tag="KLH32_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140321.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="KLH32_003652"
     CDS             3628..3801
                     /locus_tag="KLH32_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140322.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="KLH32_003653"
     CDS             3803..4147
                     /locus_tag="KLH32_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBE0140323.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="KLH32_003654"
     CDS             4151..4486
                     /locus_tag="KLH32_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBE0140324.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="KLH32_003655"
     CDS             complement(4543..6864)
                     /locus_tag="KLH32_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0140325.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="KLH32_003656"
     CDS             complement(6981..7190)
                     /locus_tag="KLH32_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140326.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="KLH32_003657"
     CDS             complement(7941..9464)
                     /locus_tag="KLH32_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0140327.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAEEDS010000128         9153 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEEDS010000128 DAEEDS010000000
VERSION     DAEEDS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.130"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..923
                     /locus_tag="KLH32_003658"
     CDS             <1..923
                     /locus_tag="KLH32_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0140328.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="KLH32_003659"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="KLH32_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBE0140329.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KLH32_003660"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KLH32_003660"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBE0140330.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="KLH32_003661"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="KLH32_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBE0140331.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="KLH32_003662"
     CDS             4446..4805
                     /locus_tag="KLH32_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBE0140332.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="KLH32_003663"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="KLH32_003663"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBE0140333.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="KLH32_003664"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="KLH32_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBE0140334.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="KLH32_003665"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="KLH32_003665"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE0140335.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2217 c         2469 g         2373 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DAEEDS010000129         9014 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEEDS010000129 DAEEDS010000000
VERSION     DAEEDS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.131"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KLH32_003666"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KLH32_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBE0140336.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KLH32_003667"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KLH32_003667"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBE0140337.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KLH32_003668"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KLH32_003668"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBE0140338.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="KLH32_003669"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="KLH32_003669"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBE0140339.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="KLH32_003670"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="KLH32_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBE0140340.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="KLH32_003671"
     CDS             5545..6018
                     /locus_tag="KLH32_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBE0140341.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="KLH32_003672"
     CDS             6047..6589
                     /locus_tag="KLH32_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBE0140342.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="KLH32_003673"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="KLH32_003673"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBE0140343.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="KLH32_003674"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="KLH32_003674"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBE0140344.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="KLH32_003675"
     CDS             7986..8243
                     /locus_tag="KLH32_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBE0140345.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="KLH32_003676"
     CDS             complement(8219..8773)
                     /locus_tag="KLH32_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBE0140346.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2188 c         2247 g         2322 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DAEEDS010000130         8989 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEEDS010000130 DAEEDS010000000
VERSION     DAEEDS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.132"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="KLH32_003677"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="KLH32_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KLH32_003678"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KLH32_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KLH32_003679"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KLH32_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KLH32_003680"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KLH32_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KLH32_003681"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KLH32_003681"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBE0140347.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="KLH32_003682"
     CDS             8509..8952
                     /locus_tag="KLH32_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBE0140348.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2356 a         2168 c         2167 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAEEDS010000131         8898 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEEDS010000131 DAEEDS010000000
VERSION     DAEEDS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.133"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..2887
                     /locus_tag="KLH32_003683"
                     /pseudo
     CDS             1..2887
                     /locus_tag="KLH32_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KLH32_003684"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KLH32_003684"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBE0140349.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="KLH32_003685"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="KLH32_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KLH32_003686"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KLH32_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="KLH32_003687"
     CDS             complement(7280..8041)
                     /locus_tag="KLH32_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBE0140350.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="KLH32_003688"
     CDS             complement(8038..8697)
                     /locus_tag="KLH32_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE0140351.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2318 g         2049 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgta gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DAEEDS010000132         8636 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEEDS010000132 DAEEDS010000000
VERSION     DAEEDS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.134"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="KLH32_003689"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="KLH32_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBE0140352.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3173)
                     /gene="hemX"
                     /locus_tag="KLH32_003690"
     CDS             complement(1968..3173)
                     /gene="hemX"
                     /locus_tag="KLH32_003690"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE0140353.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3195..3935)
                     /gene="hemD"
                     /locus_tag="KLH32_003691"
     CDS             complement(3195..3935)
                     /gene="hemD"
                     /locus_tag="KLH32_003691"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBE0140354.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3932..4873)
                     /gene="hemC"
                     /locus_tag="KLH32_003692"
     CDS             complement(3932..4873)
                     /gene="hemC"
                     /locus_tag="KLH32_003692"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBE0140355.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5260..7806
                     /gene="cyaA"
                     /locus_tag="KLH32_003693"
     CDS             5260..7806
                     /gene="cyaA"
                     /locus_tag="KLH32_003693"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBE0140356.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7846..8166)
                     /gene="cyaY"
                     /locus_tag="KLH32_003694"
     CDS             complement(7846..8166)
                     /gene="cyaY"
                     /locus_tag="KLH32_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBE0140357.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8214..>8636)
                     /locus_tag="KLH32_003695"
     CDS             complement(8214..>8636)
                     /locus_tag="KLH32_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBE0140358.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1966 a         2233 c         2220 g         2217 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     2041 gcaggtttag cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg cacgcgagtc
     2101 tgcatcagtt tttccagcat cgcctggctt tgcagggttt ccggaagatc catcgagata
     2161 ttttgctggc ttaactggtc cacctcgtcg aggaacgctt tggtggtggc atcatcagta
     2221 tcgtagtaag cacgtaccca ggtggagacg ttctccagcg cctggcgata agtctcttcc
     2281 tgatgacgcg gtacagcttg tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga
     2341 tagatatcct gatttggcgc taacagcggt acggcggtgt catcacgacg gcgaatcgta
     2401 atgaagttgt ccataaagtt ctgccagctt ttttgcagat tgatacgcca ttcgctgatg
     2461 gaactggaaa gctcttcacc gtctgaatcc atcggcgaac catcgctgtc attatcggcc
     2521 agacgcaggt tatctacctg atttgaaagc tgattaagct taaggatgat gccgtcataa
     2581 tccacctgcg atactgcaga aaggctggcg atatcatcgg taattgcccg acgaacggta
     2641 atcagactcg ggtcattcat atccgccagg ctggcgtctg cacttttcag caacgctgca
     2701 gaggtcgtga cgtcctgatc gctccacagc ttccgtccgg cgagtttcac cagaaaatcg
     2761 gcctgagcca gcagccaggt tttagcatcg ctgccggaaa tggtggcgat cttttgttgg
     2821 acttcatcca actgttttgc cagcgtttct tgctgacgat tcgcctgctt aagctgcgca
     2881 gcttgttgct taataatgcc ttccagctcg gctttttggc tctcctgggc tttttgcaat
     2941 gccgtcagtt ggttagccag ggcatcgctg gtggcggtct gattgacggc ctgttgttta
     3001 ccccagccat acaaaccgac acccgccgcc agagcaatag cgatagccac cgcgctgaga
     3061 atcaatgcag tattgttctt actctttttt tctgttgcga caggttgtga cgtggtgtcc
     3121 acggcctccc tggtctcttc aaccacggcg gaggtttttt cttgttccgt cattatggct
     3181 tcctgttatg agagttattg taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg
     3241 accttaatgt cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc actgacgacc
     3301 aatagtcgac agtgtagtaa ccagtgctca cgataccatt gtgggatcag cgaccagagt
     3361 tgctgcaaca tttcaccgct ggtaacaacg accatcgtca cctcgcggga ttgccagcgc
     3421 atcgcttctt ctgcaccatc gtaatggatt gcgcatcgtt gataacattc acaaaaagtg
     3481 acctcagcac cgcgcaccgt cagggtatcc ccaattagct cacgaccgcc attgccacgt
     3541 aatatcagcg cacgtttgcc cgcaatattt tgtaattcag gtaattgtag caagacttcg
     3601 ctgatttccc gatcctgtgg gtagagaatc ttctgtccgc ttacggtatg tagcgccagt
     3661 gcggtggtgc gtccaatggc gaaataagta ggtagttggg gccatttacg atcttgctga
     3721 tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc
     3781 tcccccagcg ctgccagttg atcagcaagt tgcggtaatt gtcgacccgg agaaaactca
     3841 atcagcggaa aatgccaggc cacctgcccc agtgtgcgca gacggctcac taactcttct
     3901 ccagcgggag acgggcgggt gaccaggata ctcatgccgg ggcctctccg ttatagactt
     3961 cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa atccccattt
     4021 gttcggcatc ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg
     4081 cgccgaccag cgcacatagc cagatttcgc catcaataag ctcggcgtag ctaccaattg
     4141 gcacctgaca tccgccttcg agacgggtat tcatggcgcg ttctgcggta acgcgcagtg
     4201 cagtttcgtg gtgattcagc gcggcaagca gctcgcgagt gcgtgtatca tcaaggcggc
     4261 attcaatacc gactgcacct tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg
     4321 cgcgaatgcg ggactccaga cctaaacgtt ttagtccggc tacggcaaga atgatggcat
     4381 cgtattcgcc gttatctagt ttgctcaggc gagtgccgac gttgccgcgc agggagcgga
     4441 taatcagatc cgggcggcgt tcagccagtt ggcactggcg acgtaaactg gacgtcccga
     4501 cgatactgcc tgctggtaac gcatccagac tgtcatagtt attggacaca aaggcatcgc
     4561 gaggatcttc acgctcgcaa atagtgacca gccccagacc ttgcgggaat tcaaccggca
     4621 catctttcat tgaatgtacg gcgatatcag cgcgattctc caggagcgcg acttccagct
     4681 cttttacaaa taagcctttt ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc
     4741 cgcgcgtcac catcggtacc agttcaacga ccaggcccgg atggctcgcc atcaacttgt
     4801 ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa
     4861 cattgtctaa catgcttgtt accgtcatta tcatccgtgg tccatcctaa catccttgcc
     4921 agagtgatgt cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct
     4981 ggcagtggat cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca gagaataaac
     5041 ggtgctacac ttgtatgtag cgcatctttc tttacggtca atcagcaagg tgttaaattg
     5101 atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta
     5161 acggttacct ttgacatacg aaatatcccg aatgccgcgt gttaccgttg atgttggcgg
     5221 aatcacagtc atgacgggta gcaaatcagg cgatacgtct tgtacctcta tattgagact
     5281 ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg
     5341 gggcctgcat tccaacaggt ctacagtcta ctgccgacat tgttgcacta tcaccatccg
     5401 ctaatgccgg gttaccttga tggtaacgtt cccaaaggca tttgccttta cacgcctgat
     5461 gaaactcaac gccactacct gaacgagctt gaactgtatc gtggaatgtc agtacaggat
     5521 ccaccgaaag gtgagcttcc aattactggt gtatacacca tgggcagcac ctcgtccgta
     5581 gggcaaagtt gttcctctga cctggatatc tgggtctgtc atcaatcctg gctcgatagc
     5641 gaagagcgcc agttactaca acgtaaatgc agcctgctgg aaagctgggc cgcctcgctg
     5701 ggtgtggaag tcagcttctt cctgattgat gaaaaccgct tccgtcataa tgaaagcggc
     5761 agcctggggg gcgaagattg tggctccacc cagcatatac tgctgcttga cgaattttat
     5821 cgtactgccg tgcgtctcgc cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa
     5881 gaagagcatt acgacgacta tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat
     5941 gaatggctgg atctcggtgg cttaagctcg ctttctgctg aagagtactt tggtgccagc
     6001 ctttggcagc tctacaagag tatcgattcc ccatacaaag cggtgctgaa aacactgctg
     6061 ctggaagcct attcctggga atacccgaac ccacgtctgc tggcgaaaga tatcaaacag
     6121 cgtttgcacg acggcgagat tgtatcgttt ggcctcgatc catactgcat gatgctggag
     6181 cgtgttactg aatacctgac ggcgattgaa gatttcaccc gtctggattt agtacgtcgc
     6241 tgcttctatt taaaagtgtg cgaaaaactc agccgtgaac gcgcctgtgt aggctggcgt
     6301 cgcgcagtgt tgagccagtt agtgagcgag tggggttggg acgaagctcg tctggcaatg
     6361 ctcgataacc gtgctaactg gaagattgat caggtgcgtg aggcgcacaa cgagctgctc
     6421 gacgcgatga tgcagagcta ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc
     6481 gtctccgcca gtccgcagga tatcggcgta ctgacgcgta aactgtatgc cgcgtttgaa
     6541 gcattgccag gtaaagtgac gctggtaaac ccgcagattt cacccgatct ctcggaaccg
     6601 aatctgacct ttatttatgt gccgccgggc cgggctaacc gttcaggttg gtatctgtat
     6661 aaccgcgcgc caaatattga gtcgatcatc agccatcagc cgctggaata taaccgttac
     6721 ctgaataaac tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg cacccgtttg
     6781 tatattaaag gtaacggcat tgtcgatttg cctaagttgc aggagatggt cgccgacgtg
     6841 tcgcaccatt tcccgctgcg cttacctgca ccgacaccga aggcgctcta cagcccgtgt
     6901 gagattcgcc atctggcgat tatcgttaac ctggaatatg acccgacagc tgcgttccgc
     6961 aatcaggtgg tgcattttga tttccgtaag ctggatgtct tcagctttgg cgagaatcaa
     7021 aattgcctgg taggtagcgt tgacctgctg taccgcaact cgtggaacga agtgcgtacg
     7081 ctgcacttca acggcgagca atcgatgatc gaagccctga aaactattct cggcaaaatg
     7141 catcaggacg ccgcaccgcc agatagcgtg gaagtcttct gttatagcca gcatctgcgc
     7201 ggcttaatcc gtactcgcgt gcagcaactg gtttctgagt gtattgaact gcgcctttcc
     7261 agcacccgcc aggaaaccgg gcgtttcaag gcgctgcgcg tttctggtca aacctggggc
     7321 ttgttcttcg aacgcctgaa tgtatcggta cagaagctgg agaacgccat cgagttttat
     7381 ggcgctattt cgcataacaa actgcacggc ttgtcagtgc aggttgaaac caatcacgtc
     7441 aaattaccgg cggtggtgga cggctttgcc agcgaaggga tcatccagtt ctttttcgaa
     7501 gaaacgcaag acgagaatgg ctttaatatc tacattctcg acgaaagcaa ccgggttgag
     7561 gtgtatcacc actgcgaagg cagcaaagag gagctggtac gtgacgtcag tcgcttctac
     7621 tcgtcatcgc acgaccgctt cacctacggc tcaagcttca tcaacttcaa cctgccgcag
     7681 ttctatcaga ttgtgaaggt tgatggtcgc gaacaggtga ttccgttccg aacaaaatcg
     7741 attggcaacc tgccgcccgc caatcaggat cacgatacgc cgctattaca gcagtatttt
     7801 tcgtgatgag cgtgccggaa agcgaggctt atccggcatg caatcttagc ggaaactgac
     7861 tgtttcaccc gcctgttgcg tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct
     7921 gcgatcgcaa atccattcat cgcctttcag gtcaaaatgg tagccgccct gtttggttgc
     7981 cagccacacc tggtgcagcg gctcctggcg gttaataatg attttgctgc cgttttcaaa
     8041 ggtaatggtc agtacgccgc cgttgatttc gcagtcgata tcgctgtcgc catccctgtc
     8101 gtccagacgc tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa attcactgtc
     8161 gttcattgtt gtatcctgtt tttaagtgat ggcggcagta tagcggcatg gggtcagggc
     8221 ttcaaagttt gcacctctgc tgctgcgttc cggcatgatt catccgtcac cggaataatg
     8281 atgtctctgt tttgaacttc acgtacgttc tcctctctga cacgagtttt cgccgggcag
     8341 ggggagggcg taatccttac ctctgtatcg gtccatgtat aaacattgcg atcgtcataa
     8401 gcgaagcgat cgtttttagt tagcgaaccg ttaactggaa taatttgcaa gctctttgac
     8461 cgtaattggc ggataaagta agcatgctgt ttgccgctat taaatgagcg atcaatgacc
     8521 aggtctggcg tatccagcgg gattatctca agctggtctt caccaaatgt cgataatttt
     8581 gttacataca gccgatcttt atattgcgca atattgccag taatcagatg gaattg
//
LOCUS       DAEEDS010000133         8579 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEEDS010000133 DAEEDS010000000
VERSION     DAEEDS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.135"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..71)
                     /locus_tag="KLH32_003696"
     CDS             complement(<1..71)
                     /locus_tag="KLH32_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140359.1"
                     /translation="MASVSISCPSCSATDGVVRNGKST"
     gene            129..377
                     /locus_tag="KLH32_003697"
                     /pseudo
     CDS             129..377
                     /locus_tag="KLH32_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
     gene            complement(746..1477)
                     /locus_tag="KLH32_003698"
     CDS             complement(746..1477)
                     /locus_tag="KLH32_003698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140360.1"
                     /translation="MHRLDEVVDTLLVLQKKHRIRFDVWQVVKRDHAIISFFDQGMNP
                     AVPRVAYWTPFRYPLLLNLASLFDNELAEKAWCARLEAHDGRSSSLFSEVCSELLARV
                     HTLGDRRYIELITDALSWAMTHFDELGYNCKTGKQKLQIMPNMVGFQFVLRGICSRLG
                     APNRKADIVVDQQSQFNTTQRELREFYYQIREMPWVHGPGLPVMDVTNMPAERWFSSR
                     VQKVQGWSWLIYISGSSNALWKGRS"
     gene            2263..3240
                     /locus_tag="KLH32_003699"
     CDS             2263..3240
                     /locus_tag="KLH32_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE0140361.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3525..4265)
                     /locus_tag="KLH32_003700"
     CDS             complement(3525..4265)
                     /locus_tag="KLH32_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0140362.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4386..4568)
                     /locus_tag="KLH32_003701"
     CDS             complement(4386..4568)
                     /locus_tag="KLH32_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HBE0140363.1"
                     /translation="MHVAQLPSLCIFLLSLRSSLPWHYPISPKNGKPALSPRHDWLNT
                     GSLLQNIPLRIWFYPQ"
     gene            complement(4911..5218)
                     /locus_tag="KLH32_003702"
                     /pseudo
     CDS             complement(4911..5218)
                     /locus_tag="KLH32_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461876.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            5711..6880
                     /locus_tag="KLH32_003703"
     CDS             5711..6880
                     /locus_tag="KLH32_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140364.1"
                     /translation="MNQKWKTLIISLLTPSSIISGIALIVLWGYFSRLGRLDIFFDVM
                     NIKSILVLVCCATILSLAMIVFIFFITSFFIPVVIPQDINNLPAYNKIQGNFLSVMML
                     SGMFPMAFIYVLYCAFDFDQNVKDNSGWLSIASMGVLIAVILAIVNKRYLEYDLSFKN
                     NRMKLLRRVQIYLVIPLSIALLVHLQLIPLEIVFSNIATSDKSVNFKVIAELAFMSYF
                     IFLLTMLPGVIYLKMNPQHKFSKRISYSFIASLMLLLIISTQITVLPVIFTHSVIKLS
                     GISDFKIHSYIIKTSEYPEEFFSNAAWDKKNIKPGDYYSVQAVSMFTTNQFILLCPKD
                     IIKFYRESWKFELLNVDFDINTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSS"
     gene            6913..7041
                     /locus_tag="KLH32_003704"
                     /pseudo
     CDS             6913..7041
                     /locus_tag="KLH32_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(7076..7369)
                     /locus_tag="KLH32_003705"
     CDS             complement(7076..7369)
                     /locus_tag="KLH32_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140365.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGARTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKCISSDLI"
     gene            complement(7475..7750)
                     /locus_tag="KLH32_003706"
     CDS             complement(7475..7750)
                     /locus_tag="KLH32_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0140366.1"
                     /translation="MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIYVLRLWHTRENR"
     gene            complement(7750..8034)
                     /locus_tag="KLH32_003707"
     CDS             complement(7750..8034)
                     /locus_tag="KLH32_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBE0140367.1"
                     /translation="MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWSDSLSTDNPLPVPR"
     gene            8265..>8579
                     /locus_tag="KLH32_003708"
     CDS             8265..>8579
                     /locus_tag="KLH32_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBE0140368.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFY"
BASE COUNT         2252 a         2010 c         1971 g         2346 t
ORIGIN      
        1 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
       61 acagaagcca caggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
      121 atcaccgtac aaatgataat gttccaggct tgctttccct gataactgca catctcaaag
      181 acctgcctga tgatggaaga aatgaagatg tgtttaaaat gttgagatca tctgccgcaa
      241 tacttcatgg gatcaataac ctgcgtaaca attacagcat ggcccatcct actgaaactt
      301 tgctgaatga agccgatgcc cgttttgcca taaatctggt tcgttccatc atgacttatg
      361 ttgatgagtt gctttagttt taataaaaat atgagactca cagcgaacag atagaacttc
      421 aacgaagaag tgaagtacgg ccagaatttc gagctctggc cctccggaag atgccggaca
      481 gacgcatcat cccgtcgtgg ccgcttccat ttttgtgtgt actcaacctg ccggatcagg
      541 atggcggcag gatttgcacc cgatgttcca gccgccttgc ctcttcctga tcccggtatt
      601 ccctcgcctt tttcatcatt tctgccgttg gctcctgcag tttgtcgaga aactccttag
      661 atcgtttagc caccgactga agggagacac tgtccgtcct gccggtattg cgattggtgt
      721 aaaccaggcg ggtaaggggg tctggttaac tccttccctt ccataaagcg tttgaagatc
      781 cagagatata tatcaaccag ctccagccct gcactttttg tacccgactg gaaaaccagc
      841 gttcagccgg catgttcgtc acatccatga ccggtaaacc gggaccgtgt acccacggca
      901 tctcacgtat ctggtaatag aactccctca gctcccgctg ggtggtgttg aactgtgact
      961 gctggtccac aacgatgtcg gctttgcgat ttggtgctcc caggcgggaa cagataccgc
     1021 gcaatacgaa ctgaaatcca accatattgg gcatgatctg cagcttttgt ttaccggttt
     1081 tgcagttata tcccagttcg tcaaagtggg tcatcgccca ggacaacgca tcggtaatca
     1141 attcaatgta acggcggtcc cctagggtat gaacgcgcgc aagcagctca ctgcagacct
     1201 cactgaacag agatgaggac cttccatcat gggcttcgag gcgggcacac cacgcttttt
     1261 cagccagttc attatcgaac aggcttgcca gattcagtag cagcgggtaa cgaaaggggg
     1321 tccagtaggc aaccctgggc actgcaggat tcatcccctg atcgaagaac gaaataatgg
     1381 cgtgatcccg tttaacgacc tgccagacat caaaccgtat ccggtgcttc ttctgcagga
     1441 caagcagcgt atcaaccacc tcatcaaggc ggtgcatgcc aagctctgcg gcgtgcagac
     1501 gctgtacttg tcaacgccac gatctttgac cgtcttatat cgacagagtt atccggcatg
     1561 gtgtcaatcc gttcacaatt tttccatcgg agcagcctac caccggtgat aaccagactt
     1621 tgcctgtagt ttgtatacac aatccctggc attgaaacac acatatgaag tgtacttcgg
     1681 atatgaccgt acgctcagga agtacggcag attccgtcaa aaattacctg taaagacaga
     1741 catcagaaaa acagtgctcg ccgcagaccg gtgaccgggc gaagcccgcg aaccggcgag
     1801 gaagcgcaat ggatctgtag ttacagtgag caggtacgcc ctgaagcctt cataatcggt
     1861 agtgtatgct gtttttctgg aatgatttat ccacatatcc ggaggcccga tccagtaaac
     1921 agatccatga atgatcaaca aaggatccat taaagatccc catatctctg caaaccttgt
     1981 cactcatggg ccgggactac gatcacataa gctgtggcat gttactgata aactgtaaca
     2041 tgctaatgat aagctgtatt cagtaatcca tatactgaag taagttaatg acataaacta
     2101 tggtcagtat gccagactca gttgttaaat acaggctgca ggtttttctt cagtcagtta
     2161 gcggagttct gacacacgat tttctgttta ttcttttact gtccacaggc tggaggcttt
     2221 ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa gtcgtccggt
     2281 gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc gctgatgcgt
     2341 ctgggcgtct ttgtaccgac ccttaagtca ctgaagaaca gtaaaaaaaa tacactgtca
     2401 cgcaccgatg ccacggaaga actgacgcgt ctttctctgg cccgtgctga gggattcgat
     2461 aaggttgaga tcaccggccc ccggctggat atggataacg atttcaagac ctgggtgggg
     2521 atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact gccttttgtt
     2581 gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct gcgtgagcgc
     2641 atcagccctt ccctgaagcg cattgccggt accgtgatct cgttttcccg taccgatgag
     2701 aagcacaccc gggaatacat cacccatctg gtacagtcag cctactacga tactgaacgg
     2761 gatattgttc agttacaggc tgatccccgc ctgtttgaac tgtaccagtt tgacagaaag
     2821 gtccttcttc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc ccaggcactc
     2881 tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc gcggctgcgt
     2941 gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag acgggcaatg
     3001 gagcagctgc gcgagattgg atatcttgat tacacggaga tccagcgggg gcggacaaaa
     3061 ctcttctgca ttcactaccg gcgtccccgg ttaaaagcac cgaatgatga gagtaaggaa
     3121 aatccgttgc caccttcacc tgcggaaaaa gtcagtccgg agatggcgga gaaacttgcc
     3181 ctgcttgaaa aactgggcat cacgctggat gacctggaaa aactcttcaa atcccgctga
     3241 acataaactg tagtcagtga agagcgctcc tttactgact acagcttata ttaaccggtg
     3301 cagtgagtgg tctgctcact gcagtttata ttctgtttcc tgcagtgctg cctgtggctg
     3361 agctgccatc tgcctgtccc ttacgtaagt gatcccgtaa cctgctgctg aggcattgct
     3421 cccttcataa aacatgactt actcactaca gcttatatac atgctccagc ttatgttatg
     3481 tctgttctgc tgaccacagc ttatataagg aagcgcgtat gccgtcacct caggggaggc
     3541 agtgtacgca ggatctccgc agcatcccgc ccgtcacctg tgaaaggcac tgccagcgtg
     3601 gcagccatat ccagtgcaaa cactctggta taaacctcca tcgaacgtgg atccctgtga
     3661 ccagccagtg cctggatgac tttccggggc tggcggtgat agagcatgtg catgatatag
     3721 ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt gtaccccgtc tgcttcggcc
     3781 cgtctgacag cctgcttcag ccagttgcgc atggtttcgt cggtcacagc ccataatggt
     3841 tcacgacgac ggggccgggt ggtgatcatc cagctttcca tctgcctgac atagcttata
     3901 tctgtcagcg gaaccaggcg gacttcatct tttggcgggc gtccgcgtcg cgcacgcact
     3961 ttttcggaca ggatccgcac aaacggtctt actccattca ggtcaaatga ttccggcgtc
     4021 agcatccggg cttcgccaat acgcattccg gtattccaga gggtggcgaa cagcatatgg
     4081 tgacgctgat ccggcatata gaagagaagg gcactcactt ccggggccag caggtaggcc
     4141 ggtgtggccc ccgtggaagt ggccattctt ctcagggaga gcgctgttgc aaaatccacc
     4201 cccggcgcaa tgggtaacag ggagacgcgt tctggtgaat tctgcagggg aatgacattg
     4261 ttcatgagtt atttttcacg tagcacacgc actgtttatg gatacaggta ttctgacctg
     4321 aacgcccgga tccttccacg acaataaatc gcagcgcgcg gatccttttg tttgcttatc
     4381 acgcattatt gcgggtaaaa ccaaatacgt aaaggtatgt tttgcagcag ggatcccgta
     4441 ttaagccagt cgtggcgcgg gctgagggct ggtttgccgt ttttgggaga aatggggtaa
     4501 tgccagggca aagatgatcg caaggaaagg agaaaaatac acagagaggg gagttgcgct
     4561 acgtgaattc tggctaggta cacccctgta accctgccag ttttcacgca acgcaatgtg
     4621 gtggctgatt actgtcagtt gaaatgacgg ctggcatcct gatgctgact taatatacga
     4681 ataataacaa tttcagtatc agtctgcaga aaataaacca tatgccgttc aacgggtagt
     4741 gcgaatgtgt attccccagt tcaggagggg gagtaccgat attatgatta ctcataacct
     4801 gaaaaatgtc ggatatctga ttaatatatt tatcagccta ctcttcaccg aaatggtggt
     4861 acccgtaatg ctagatatcc tccagatcct gactggcttt aggtgtgagt ttaacggttt
     4921 tcaccggaag ccctcgcgcc agctttcact tttttcagaa acgcattttt ttcccacgac
     4981 acaggttcac cgctgctcaa gccttctgcc agtaaatccc gcaggacacc tggagacgtg
     5041 actctgcctg tttctctcga aggagacgaa gtgactcgcg gattacctca ctctgtgtac
     5101 gataatcacc tgattctatg agagattcta tgtactccca gagttcatct ccgagagcaa
     5161 ctgtcattgt tctggccata tcaagcctcg tgattgtaag tttaaatttt acatgcagtt
     5221 tattcgatac tcaaaggtgc ttccagtaca gaacatttgg gagaattggg gtaatgcctg
     5281 gaacagacag atacggagaa aaaccctgca gataacctta tacgacaact gaaggtgtct
     5341 gatgccatat tcttagctac ttctctgtct tcaggcggct tagtcacaac ataagaccga
     5401 tgcataataa gcatattttt tcatgttatg gtcttgttca tgcatgaact attatctatt
     5461 cagtcacttt taaagtttta tatcatactg catatgatat atatcatctc ctatattcag
     5521 actaaaaata ttatgacgat ggtcaaatga atttagtgaa aacgctacct aaaacaatat
     5581 gcttatgata taacttatca agaatatgcg acaatcttct tgaaaggtta cggagaagag
     5641 tcagataatg aagtgttata actcattcag cttttttatg ttctctttat agtaaaacca
     5701 ggagattaag atgaatcaga agtggaagac gttaattatt tcattattaa caccatcaag
     5761 tattatatca gggattgcgt tgatagttct ctggggatat ttcagtcgcc tgggccgact
     5821 agatattttc tttgatgtaa tgaatattaa aagcatcttg gtattggttt gttgtgcaac
     5881 tatactatca ttggctatga tagtatttat cttttttata acctcttttt ttatacccgt
     5941 agttattcct caggatataa ataatcttcc tgcttacaat aagattcagg gtaatttttt
     6001 atctgtgatg atgttatctg gcatgttccc catggccttt atatatgtgt tatactgtgc
     6061 atttgatttt gatcagaatg ttaaagataa ctctggctgg ttatctattg caagtatggg
     6121 tgtactgata gctgttattt tagccatagt aaataagagg tatcttgaat atgacttatc
     6181 gtttaaaaac aatagaatga aactgttgag acgtgttcag atctatcttg tcattccgtt
     6241 gtctattgct ttactggtgc atcttcagtt aattcctctg gagatagttt ttagtaatat
     6301 tgcaacttca gataagagtg ttaatttcaa agtgatagct gaactggctt tcatgtcata
     6361 ttttatcttt cttctgacaa tgcttcctgg agtcatttat ctgaaaatga atccacagca
     6421 taaattttca aaaagaatta gctattcatt tatcgcatcc ttgatgttat tattaataat
     6481 atcgactcaa ataaccgtgt taccagtgat ttttacccat tccgttatta agctatcagg
     6541 tataagtgac ttcaaaatac atagctacat tattaaaaca agtgaatatc ctgaagagtt
     6601 tttttccaat gcagcatggg ataaaaaaaa tataaaaccg ggggattatt actctgtgca
     6661 ggctgtttct atgtttacga caaaccagtt tattctcctt tgtcctaagg atattataaa
     6721 gttttatcgg gaaagctgga agttcgagtt gcttaatgta gacttcgata taaataccag
     6781 gaagaaactc caggaagaag ctgcatattg cgtaccaatt tctgcaatat ctgtaaaacg
     6841 atgggatatg cctcttcagg gctcgaaacc ctcaagttag aaggcaacta gggcgactgt
     6901 attctgtgac gaaatgaatg gctggctcac tataataatg agcgaaccca tcagggaaaa
     6961 atgtgcaatg ggcggacgcc aatggaaacg ttactcgatg gaaaacgcat ctgggccgag
     7021 aaaaatttaa gccagatgta atctgacaga tacctgtata aataaccgat aactgtcaga
     7081 tcaggtctga gctaatacat ttcaataaag gtacttgctc gcgctctgtc attttctgaa
     7141 actcttcatg ctgcatttcg attttccttc taagatcata actcgcatac ctgttctttt
     7201 tatgtatatc tatatctatt tttgtgacga gttcaaacca caatgttcgc gctccccatg
     7261 cagcgttatg aaacatttcc gcaacggcta tagagtctgt attaactgct tcagtcatgt
     7321 agtcgctaat acgttcagcg gtaattttcg ttatttcatc gtaattcatt ttatctccta
     7381 caagattatt taatggaatg caaaatatct ggtctgataa taatgttcca gaattaatat
     7441 ctgcaggcta tgttaagttg tccccacaca caatctacct gttttctcgt gtgtgccaca
     7501 gacgcaatac ataaatagtc tggccattaa tttcgtaacg gatttcgtac tcgccagcaa
     7561 aaatccgtct gacctccctg ggttcaaact gaaacaactg ttctcccata cgtggatgag
     7621 ttaacagaat gaccggtgcc tgtgtcaggg actgtaccgt tctggcggca gcaggtttac
     7681 tggccagcac cagaaaatca aataaccgcg acaaatcaga aagcgcctta ctggtccact
     7741 tcagttccat cagcgtggca ccggtaacgg attgtcagta ctgaggctgt ccgaccaggc
     7801 ctgtacagcc tgatggtcga taacctgtcc ggatgtcaca tcgtccaggg cttccagcgt
     7861 caggcgatta cgctcctcct cctgggcaag ccatgcagaa agcgcctgtt tgataaccca
     7921 gccccgggaa cgttccagtc tggcggccat ctggtcgact ttatcagcca taggtaatgg
     7981 cacatgcgcg gtaattactt ttgttgcttg tgcggcattg tttttcattt gcatatccga
     8041 ttagttgtta atcatattta atcatagtga ttactttcag acggaacaag gcaaataatg
     8101 cgcgtcagcg catgtaaggc ggcatacgga ggttacatgt agcacaggtg taaaaccgcc
     8161 gaagcccggc gtaagccggt actgattgat agatttcacc tggcactgtt gcaaatagtc
     8221 ggtggtgata aacttatcat ccctttttgc tgatggagct gcacatgaac ccattcaaag
     8281 gccggcattt tcagcgtgac atcattctgt gggccgtacg ctggtactgc aaatacggca
     8341 tcagttaccg tgagctgcag gagatgctgg ctgaacgcgg agtgaatgtc gatcactcca
     8401 cgatttaccg ctgggttcag cgttatgcgc ctgaaatgga aaaacggctg cgctggtact
     8461 ggcgtaaccc ttccgatctt tgcccgtggc acatggatga aacctacgtg aaggtcaatg
     8521 gccgctgggc gtatctgtac cgggccgtcg acagccgggg ccgcactgtc gatttttat
//
LOCUS       DAEEDS010000134         8415 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEEDS010000134 DAEEDS010000000
VERSION     DAEEDS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.136"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="KLH32_003709"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="KLH32_003709"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE0140369.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="KLH32_003710"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="KLH32_003710"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBE0140370.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="KLH32_003711"
     CDS             3115..3399
                     /locus_tag="KLH32_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE0140371.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KLH32_003712"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KLH32_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBE0140372.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KLH32_003713"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KLH32_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBE0140373.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KLH32_003714"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KLH32_003714"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE0140374.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2140 g         2003 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta gcgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gctta
//
LOCUS       DAEEDS010000135         8396 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEEDS010000135 DAEEDS010000000
VERSION     DAEEDS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.137"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            4..817
                     /gene="ttdA"
                     /locus_tag="KLH32_003715"
                     /pseudo
     CDS             4..817
                     /gene="ttdA"
                     /locus_tag="KLH32_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            814..1419
                     /gene="ttdB"
                     /locus_tag="KLH32_003716"
     CDS             814..1419
                     /gene="ttdB"
                     /locus_tag="KLH32_003716"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBE0140375.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1467..2930
                     /gene="ttdT"
                     /locus_tag="KLH32_003717"
     CDS             1467..2930
                     /gene="ttdT"
                     /locus_tag="KLH32_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBE0140376.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KLH32_003718"
     CDS             complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KLH32_003718"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBE0140377.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4224..4439
                     /gene="rpsU"
                     /locus_tag="KLH32_003719"
     CDS             4224..4439
                     /gene="rpsU"
                     /locus_tag="KLH32_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBE0140378.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4550..6295
                     /gene="dnaG"
                     /locus_tag="KLH32_003720"
     CDS             4550..6295
                     /gene="dnaG"
                     /locus_tag="KLH32_003720"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0140379.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6490..8331
                     /gene="rpoD"
                     /locus_tag="KLH32_003721"
     CDS             6490..8331
                     /gene="rpoD"
                     /locus_tag="KLH32_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBE0140380.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2254 c         2253 g         1862 t
ORIGIN      
        1 gcagtgaata agttgacaga gattgtcgct aactttaccg ccatgatttc tacccgaatg
       61 cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc gtcgatgggg
      121 aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct gaatcgtcct
      181 gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg cttcccactg
      241 cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt caaagcgcca
      301 ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa taccggtagc
      361 ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga aatcgaagtt
      421 tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat gccgtcagaa
      481 ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc
      541 tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc cgccgtactc
      601 tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa agcggcagaa
      661 ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca agggctgacc
      721 ggcaacagtt cagtgatggg cgcatcgtcg cggcacgctg ctggttcatg ccgatctcac
      781 ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa agatcctgac aaccccgatc
      841 aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga tctacctgac cggtacgctg
      901 gtgacctgcc gcgacgtttg tcaccgccgt ttgatcgaac tgaaacgtcc gatcccttac
      961 gatctcaacg gcaaagcgat tttccacgct ggccccatcg tgcgcaaaaa cggtgacaaa
     1021 tgggagatgg tctccgtcgg cccgacaacc agtatgcgta tggaaagttt tgaacgtgaa
     1081 tttattgagc agaccggcgt aaaactggtg gttggcaaag gtggtatggg gccgctgacc
     1141 gaagaaggct gccagaaatt caaggcgtta catgtgattt tcccagcagg ctgcgcggtg
     1201 ctggcggcaa cccaggtaga agagattgaa gaagtgcact ggacagaact cgggatgccg
     1261 gagtcactgt gggtctgccg ggtcaaagag ttcggcccgc tgattgtctc tattgatacc
     1321 cacggcaaca acctgatagc cgaaaacaaa aagctgttcg ccgaacgccg cgatcccatc
     1381 gtggaagaga tctgcgagca cgtccattac atcaaataac cctcccggag aggctcaccc
     1441 ctctcctttt tcgcaggcat aacacgatga aaccttccac tgaatggtgg cgatatcttg
     1501 cgccgctggc ggtcatcgcc attattgctc tacttccggt tcccgcaggg ctggagaacc
     1561 atacctggct ctactttgcc gtttttactg gcgtgatcgt tggactgatc ctcgaacccg
     1621 tgccgggtgc cgtggtggcg atggtgggta tctccatcat cgccatactc tctccctggc
     1681 tgctgttcag ccccgaacag ctcgcccagc caggctttaa gttcaccgcc aaatccctct
     1741 cctgggcagt ttctggtttt tctaattcgg ttatctggct cattttcgcc gcctttatgt
     1801 ttggcacagg ctatgaaaaa accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa
     1861 agatggggca tcgcacgctg tttctcggct atgcggtgat gttctccgag ctgatcctgg
     1921 cacctgtaac accgtccaac tcggcgcgtg gtgcgggtat tatctatccc atcatccgta
     1981 acctgccacc gctctatcaa tcacaaccaa acgacagcag ttcgcgcagc attggctcgt
     2041 acatcatgtg gatggggatt gttgccgact gcgtgaccag cgccattttc ctgacggcga
     2101 tggcacctaa cttgctgtta attggactga tgaaaagcgc atctcacgcc acgctgagtt
     2161 ggggcgactg gttcctcggg atgttgccgc tcagcatttt actggttctg ctggttccct
     2221 ggctggctta cgtgctgtac ccgccggtac tgaagtctgg tgatcaggtg ccgcgctggg
     2281 cagagacgga actgcaggca atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc
     2341 tgatggtagg cgcgctggtg ctgtggattt tcggcggtga ttatatcgat gccgcgatgg
     2401 tcggttacag cgtggtggcg ctgatgctgc ttctgcgcat tatcagttgg gacgacattg
     2461 tcagtaataa agcggcatgg aacgttttct tctggctggc ctcgcttatc accctcgcga
     2521 ccggactcaa caataccggt tttattagct ggtttggcaa actgttagca ggcagcttaa
     2581 gtggttattc gccgacgatg gtgatggtga cgttgattgt ggtgttttat ctactgcgct
     2641 actttttcgc cagcgccacg gcgtatacct gcgccctcgc accgatgatg atcgccgccg
     2701 cgctggcgat gccggaaatc ccgctgccgg tgttctgcct gatggttggc gcggcaattg
     2761 gtctggggag cattcttaca ccatacgcca ccggacccag tccgatttac tacggtagtg
     2821 gttatctgcc aacggcggat tactggcgac tgggggcgat ttttgggctg atattcctcg
     2881 tgttgctggt gattaccggc ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg
     2941 tgccggaaat tgctctccgg cacccaatag ttttacgcag ccggtaactc cgccagcggc
     3001 cagcgcggac gcacgctaac accgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt
     3061 ccggcatagg cgatcatcgc gccgttatca gtacaaaatt ccggacgcgc gtagaacact
     3121 tcgccgcggc gttttttcat catttcagcc agcttcgccc gtaacgtgcg gttagcactc
     3181 acgccgcccg ccatgaccag tcgcttaaag cccgtctgat ccaacgctcg cttacactta
     3241 atcatcaacg tatcgaccac cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg
     3301 tcgtcggtgc cgttgtcacg aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg
     3361 aaatccagcc ccggacggtc ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc
     3421 tgagccgcca ttttcgacag taacggtccg ccaggataat ccagccccag cagcttcgcg
     3481 gttttatcaa acgcttcacc ggcggcatca tcgatagact cgccgagcag ctcgtactga
     3541 ccaatgccag tcacgctgat taactgcgta tgaccgccgg acaccagcag cgcgacaaac
     3601 ggaaattccg gcgggttatc ttccagcatc ggcgctaaca gatgcccttc catatggtgt
     3661 accgggattg ccggaacgtt ccaggcaaac gccagagaac gccccacggt tgcgccaacc
     3721 agcagtgcgc cgactaatcc agggcctgcg gtataggcca cagcatcaat gtcttttgcc
     3781 gttaaaccag actcctttag cgccgcctgg atcaacggta cggttttacg cacatgatcg
     3841 cgggaggcca gttcaggcac gacgccgccg tagtcagcgt gcaatttcac ctgactatac
     3901 aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag
     3961 gaagtttcaa tacccagtac acgcatgact tgttttacct cgctttatta ccgcgcagtg
     4021 taggaccaat gcgggttgat gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac
     4081 tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg tgctttacaa agcagcagca
     4141 attgcagtaa aattccgcac cattttgaaa taagctggcg ttgatgccag cggcaaaccg
     4201 aattaatcaa aggtgagagg cacatgccgg taattaaagt acgtgaaaac gagccgttcg
     4261 acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc aggtgttctg gcggaagttc
     4321 gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa gcgcgctaaa gcttctgcag
     4381 tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg ccgcactcgt ctgtactaat
     4441 tccccgagag cgttgctctc cgatcagacc gagttgtagt tgtaaggccg tgcttccgaa
     4501 aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa tcggggccta tggctggacg
     4561 aatcccacgc gtattcatta atgatctgct ggcacgcact gacatcgtcg atctgatcga
     4621 tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac gcgtgttgtc cattccacaa
     4681 cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag ttttaccact gctttggatg
     4741 tggcgcgcac ggcaacgcga tcgacttcct gatgaactac gacaagctcg agttcgtcga
     4801 aacggtcgaa gagctggcag caatgcacaa tcttgaagtg ccatttgaag caggcagcgg
     4861 ccccagccag atcgagcgcc atcagaggca aacgctttat cagttgatgg acggtctgaa
     4921 tacgttttac caacaatctt tacaacaacc tgttgccacg tctgcgcgcc agtatctgga
     4981 aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg attggttttg cgccccccgg
     5041 ctgggacaac gtcctgaagc ggtttggcgg caatccagaa aatcgccagt cattgattga
     5101 tgcggggatg ttggtcacta acgatcaggg acgcagttac gatcgtttcc gcgaacgggt
     5161 gatgttcccc attcgcgata aacgcggtcg ggtgattggt tttggcgggc gcgtgctggg
     5221 caacgatacc cccaaatacc tgaactcgcc ggaaacagac attttccata aaggccgcca
     5281 gctttacggt ctttatgaag cgcagcagga taacgctgaa cccaatcgtc tgcttgtggt
     5341 cgaaggctat atggacgtgg tggcgctggc gcaatacggc attaattacg ccgttgcgtc
     5401 gttaggtacg tcaaccaccg ccgatcacat acaactgttg ttccgcgcga ccaacaatgt
     5461 catttgctgt tatgacggcg accgtgcagg ccgcgatgcc gcatggcgag cgctggaaac
     5521 ggcgctgcct tacatgacag acggccgtca gctacgcttt atgtttttgc cggatggcga
     5581 agaccctgac acgctggtac gaaaagaagg taaagaagcg tttgaagcgc ggatggagca
     5641 ggcgatgcca ctctccgcat ttctgtttaa cagtctgatg ccgcaagttg atctgagtac
     5701 ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca ttgatatcgc aagtgccggg
     5761 cgaaacgctg cgaatatatc ttcgtcagga attaggcaac aaattaggca tacttgatga
     5821 cagccagctt gaacgattaa tgccaaaagc ggcagagagc ggcgtttctc gccctgttcc
     5881 gcagctaaaa cgcacgacca tgcgtatact tatagggttg ctggtgcaaa atccagaatt
     5941 agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat aagctccctg gacttggctt
     6001 attcagagaa ctggtcaaca cttgtctctc ccagccaggt ctgaccaccg ggcaactttt
     6061 agagcactat cgtggtacaa ataatgctgc cacccttgaa aaactgtcga tgtgggacga
     6121 tatagcagat aagaatattg ctgagcaaac cttcaccgac tcactcaacc atatgtttga
     6181 ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt gagcgcacgc atggtttaag
     6241 caacgaagaa cgcctggagc tctggacatt aaaccaggag ctggcgaaaa agtgatttaa
     6301 cggcttaagt gccgaagagc gatcgggaag cccccgacag ccgcactgag aggcagcggc
     6361 aaatatataa gtacgccctc gtaattatcg ttggcggtaa acaaccgttg gatttcagcg
     6421 ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa cctcatgaaa taagtgtgga
     6481 taccgtctta tggagcaaaa cccgcagtca cagctgaaac ttcttgtcac ccgtggtaag
     6541 gagcaaggct atctgaccta tgccgaggtc aatgaccatc tgccggaaga tatcgtcgat
     6601 tcagatcaga tcgaagacat catccaaatg atcaacgaca tgggcattca ggtgatggaa
     6661 gaagcaccgg atgccgatga tctgatgctg gctgaaaaca ccgcggacga agatgctgcc
     6721 gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa tcgggcgcac gactgacccg
     6781 gtacgcatgt acatgcgtga aatgggcacc gttgaactgt tgacccgcga aggcgaaatt
     6841 gacatcgcta agcgtattga agacgggatc aaccaggttc aatgctccgt tgctgaatat
     6901 ccggaagcga tcacctatct gctggaacag tacgatcgtg ttgaagcaga agaagcgcgt
     6961 ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag aagaagatct ggcacctacc
     7021 gccactcacg tcggttctga gctttcccag gaagatctgg acgatgacga agatgaagac
     7081 gaagaagatg gcgatgacga cagcgccgat gatgacaaca gcatcgaccc ggaactggct
     7141 cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa cgcgtgacac catcaaagcg
     7201 aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga aactgtctga agtattcaaa
     7261 cagttccgcc tggtgccgaa gcagtttgac tacctggtca acagcatgcg cgtcatgatg
     7321 gaccgcgttc gtacgcaaga acgtctgatc atgaagctct gcgttgagca gtgcaaaatg
     7381 ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa ccagcgatac ctggttcaac
     7441 gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc acgatgtctc tgaagaagtg
     7501 catcgcgccc tgcaaaaact gcagcagatt gaagaagaaa ccggcctgac catcgagcag
     7561 gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga aagcccgccg tgcgaagaaa
     7621 gagatggttg aagcgaactt acgtctggtt atttctatcg ccaagaaata caccaaccgt
     7681 ggcttgcagt tccttgacct gattcaggaa ggtaacatcg gcctgatgaa agcggttgat
     7741 aaattcgaat accgccgtgg ttacaagttc tccacctacg caacctggtg gatccgtcag
     7801 gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc gtattccggt gcatatgatt
     7861 gagactatca acaaactcaa ccgtatttct cgccagatgc tgcaagagat gggccgtgaa
     7921 ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg aagacaagat ccgcaaagtg
     7981 ctgaagatcg ccaaagagcc aatctccatg gaaacgccga tcggtgatga tgaagattcg
     8041 catctggggg atttcatcga ggataccacc ctcgagctgc cgctggattc tgcgaccacc
     8101 gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc tgaccgcgcg tgaagcaaaa
     8161 gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc acacgctgga agaagtgggt
     8221 aaacagttcg acgttacccg cgaacgtatc cgtcagatcg aagcgaaggc gctgcgcaaa
     8281 ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc tggacgatta atcggtaggc
     8341 cggatcaggc gttacgccgc acccggcact agtctctctg cacaaacgcc accttt
//
LOCUS       DAEEDS010000136         8356 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEEDS010000136 DAEEDS010000000
VERSION     DAEEDS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.138"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            66..314
                     /locus_tag="KLH32_003722"
     CDS             66..314
                     /locus_tag="KLH32_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBE0140381.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            379..747
                     /locus_tag="KLH32_003723"
     CDS             379..747
                     /locus_tag="KLH32_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE0140382.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            841..1494
                     /gene="nfsB"
                     /locus_tag="KLH32_003724"
     CDS             841..1494
                     /gene="nfsB"
                     /locus_tag="KLH32_003724"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBE0140383.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1602..2849
                     /gene="ybdG"
                     /locus_tag="KLH32_003725"
     CDS             1602..2849
                     /gene="ybdG"
                     /locus_tag="KLH32_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBE0140384.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIRRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="KLH32_003726"
     CDS             complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="KLH32_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBE0140385.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="KLH32_003727"
     CDS             complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="KLH32_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBE0140386.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="KLH32_003728"
                     /pseudo
     CDS             complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="KLH32_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2041 a         2326 c         2032 g         1957 t
ORIGIN      
        1 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
       61 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
      121 tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg
      181 tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt
      241 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
      301 cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg catactttgc ttattctcct
      361 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctagct
      421 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
      481 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
      541 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
      601 tatcacatga ataaaaagca ctggatttcg gtgtattccg gcgaggaaat ctccgaagcg
      661 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
      721 gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat agcgcatttt tctcgcttac
      781 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tacttacaca tggagtcttt
      841 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
      901 aaacttaccc cggaacaggc cgagcagatc aaaacgctac tgcaatacag cccatccagc
      961 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     1021 gccaaatccg ctgccggtaa ttatgtgttc aacaaacgta aaatacttga tgcctcgcac
     1081 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     1141 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     1201 cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     1261 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     1321 gcggtgccca tcgaaggttt tgacgccgca atcctcgatg cagaatttgg tctgaaagag
     1381 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     1441 gctacgctgc cgaaatctcg tctgccgcaa aacaccacct taaccgaagt gtaattctct
     1501 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     1561 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     1621 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     1681 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     1741 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     1801 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     1861 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     1921 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     1981 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     2041 tcggcgcaat cctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     2101 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     2161 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     2221 ggctggaaat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     2281 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     2341 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aggcgcagta
     2401 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     2461 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     2521 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     2581 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgacct
     2641 taatggtacg ccaactggct ccaggtgata acggtttacc gctcgagatc tacgcgttta
     2701 ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     2761 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     2821 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     2881 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     2941 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     3001 catcgtgcac atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa
     3061 cggatagagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ccgcgcgaaa
     3121 acgcagatgc gccaggcaga tcataatcca gttcaacagc aacgttgcta ccaccagcgc
     3181 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt
     3241 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     3301 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     3361 cccgctgtta tacaccgaca gcgatgccac tagaatgacg aagttcagcg cagaagctac
     3421 cacattgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     3481 cacccacgga tagagtgcca gtaaaaccac cagtgaaccg atataaaaca gcaggatgcg
     3541 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     3601 tgtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     3661 cagcccattc cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc
     3721 tttctcgccg ccgtaaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     3781 cgccagcact ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac
     3841 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     3901 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     3961 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     4021 ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc accatctcgc caagctggcg
     4081 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     4141 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc
     4201 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     4261 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     4321 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     4381 gaggtatcct gctttaattc cgtacccgat gtcggtgcag ccacatcagc ttatacgccg
     4441 ccgggataat aaacagcgac agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg
     4501 caatccggct catcacctct gaaccagccc ccgttcccca cagaatcggc agcagaccgg
     4561 cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg caggaccgcg ccgtgataca
     4621 acgcctcatc cagcttctgc tcgctgaatg tttgtggatt attcaacgac ggctcggcct
     4681 ctatggcgtg acgtaaatac atcagcatca ccacgccaaa ttcggcggcg accccggcaa
     4741 gggcaataaa gccggtaccc gtcgccacag aaagatgaaa gcccatccac cacagtaacc
     4801 agatgccgcc caccagcgca aacggtacgc tgctgataat cagcaacgct tcgcccaccc
     4861 gacggaatgc cagatacaac agcacgaaga tgatcatcag tgtcatcggc accatgagtt
     4921 tcagcttatg gttggcgcgc tccagtagct cgaactgccc ggagaatgcc acgctggtgc
     4981 ccggttttaa ctgcactttt tcagctatcg ctttttgcaa atcgtgaacc accgacacca
     5041 tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc tcggttttca
     5101 gcatcgacgg tccggtagag actttaacgt cggccacgtc tgccagggtg atttgctgct
     5161 tcatcggcgt caggatcggc aactggcgca gtgcctgcgg actatcgcgc cagctttgcg
     5221 gataacgcag attaattgga taacgggcaa tcccttccac cgtttcgcca accatcgccc
     5281 cgcccaccgc agaagtcaca aacaactgca catccgccac cgtcataccg taacgtgcgg
     5341 ctttttcacg gttaatctca acgttgatat agcgcccacc ttccagccgc tcagcaagcg
     5401 cagaagctac gcctggcacc gttcgcgcca cttcttcaat ttgctcagcc atcgcgtcga
     5461 tatccgccag cacagtgccg gaaactttaa tgccgatggg gcttttaatg ccggttgaga
     5521 gcatatcgat acggttacga attggcggca cccacagatt cgccagcccc ggcagacgca
     5581 cggtgttatc cagttcctca atgattttgt ccattgtcat gcctggccgc cactgatcct
     5641 gtggcttaag ctggatggtc gtttctacca tctccagcgg agcagaatcg gtggcggttt
     5701 ccgctttccc ggttttgcca aatacccgcg ccacttcagg tacgctcata attagcttgt
     5761 cggttttttg cagcatactc gccgcctctg ctgcggaaat ccccggcagc gtcgatggca
     5821 tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc cccgccaact ttattgagcg
     5881 gccagagaac cgtcagcacc gaaagcgccg ccaccagcag cgtggttttc ggccagtgca
     5941 gtactttcag caacagcgga tgataaacac gaatcaaaaa gcgattgagc ggattactgc
     6001 tttccggcgg aattttgcca cggatccagt aacccatcag gatagggatc actacgatcg
     6061 ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa cgccaacggg ccaaacagac
     6121 gcccttcctg cccttccagg gtgaagatcg ggataaacga caacgtgata atcagcagac
     6181 tgataaacag cgccggcccc acttcaacag acgcatcggt gatcacctgc cagcgcgttt
     6241 tattatccag cgtggcgtca ggatgctggt gctgccactc ttccagccgt ttatgcgcat
     6301 tctcgatcat gacgatagcg gcatcgacca tcgccccgac ggcaatcgca atgccaccca
     6361 gcgacataat attggcattc agcccctgga agggcatgac aataaaagca atacacaacc
     6421 ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac atgccagaga aacagcgcgc
     6481 agactaccgc caccacaata aactcttcca gcaacttgcc gctgaggttg tcgatggccc
     6541 ggtcaatgag ctggctgcgg tcgtatgttg taactatctc cacgccttcc ggcagactac
     6601 ttttcagcgt ttccagtttg tccttcacgg cggcgatcac ttctcgggcg tttttgccgg
     6661 atcgcaggat caccaccccg cccgccactt cgccttcgcc gtttagttcg gcaatgcccc
     6721 ggcgcatctt cgggccaatc tggaccttcg caacatcgcg cagataaacg ggcacgccat
     6781 tttcactggc ttttaaaacg atgtgattaa agtcgtcgag cgtttgtaga tagccgctgg
     6841 cgcgcaccat atattctgct tccgccagtt cgattgacga accgcccgct tcctggtttg
     6901 aagcatccag cgcgcttttt acttcggcga gactgatgcc atactgcgcc aggcgctgag
     6961 gatcgataac cacctgatac tctttcacca caccgcctac cgacgccact tccgcaacgt
     7021 caggaatggt tttcagctca tatttgagaa accagtcctg caatgagcgt aaatcggcaa
     7081 gatcgtgctt accgctgcga tccaccagtg catattcata gatccagcca acacccgtgg
     7141 catctggtcc cagctcggcg ctgactcccg caggcagctt accctgtacc tggttgaggt
     7201 actccagcac ccgcgaacgc gcccagtacg gatcggtgcc atcttcgaaa atgacataca
     7261 cataagagtc gccaaactgc gagaaaccac gcacagtctt cgcgccaggc accgacaaca
     7321 tggtggtggt tagcggataa gtcacctgat tttcaacgat ttgcggtgcc tggccgggat
     7381 agctggtttt aataatcacc tgcacatcgg agagatccgg caacgcatcc actggcgtat
     7441 taatgatggt ccaggtgccc cagatgctca gaaacaacgc gcccatcagc accaaaaaac
     7501 ggttcgccac cgagcgacga ataatccatt caatcattgg ttattccctc aatgcgcatg
     7561 ggtagcactt tcagagcgca tccgctccag tgcgccagaa atattggctt cggaatcaat
     7621 caggaacagg ccgccggaaa ccaccttttc accttccgcc agaccagagc gtaatgcggt
     7681 gacgccttgc gatgcctgga aaacagcaac gcgtttcggt acaaagcgcc cgtcggcatc
     7741 aacggtaatc acccgctgtt cgttgccggt atcaatcagc gcctgtgacg gaatgagcag
     7801 catcggttcg ctggcggtgt tgagttgcaa ccaggcgttc atgcccggtt ttagcgcctc
     7861 gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg
     7921 tagcagcgtc catttgcgga tggtgagtgt tttatccggt cgcgccggaa cggtgagggt
     7981 aaactgcgag gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca
     8041 caccgggtcc ataccctgaa ttcgcgatta cgcgcaatcc ccctgagaac cgccatgaac
     8101 aagggtttca tgatttgtga ttttagtttg gtacgcaatt tggtacacaa cactattttc
     8161 acttcagcgg gtagtcatca aactcaccat aacgagcatc gttgatgatg tatgtgatca
     8221 ccacaaatat cacatctgga ctagttccgt cttcatgcgc aggtagactg gccccctgaa
     8281 tctccagaca accagtatca cttatttaag tgatagtctt aatactagtt tttagacttg
     8341 tcattggaga acagat
//
LOCUS       DAEEDS010000137         8047 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEEDS010000137 DAEEDS010000000
VERSION     DAEEDS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.139"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            52..585
                     /gene="ymdB"
                     /locus_tag="KLH32_003729"
     CDS             52..585
                     /gene="ymdB"
                     /locus_tag="KLH32_003729"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBE0140387.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            527..2008
                     /gene="clsC"
                     /locus_tag="KLH32_003730"
     CDS             527..2008
                     /gene="clsC"
                     /locus_tag="KLH32_003730"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBE0140388.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2016..3173)
                     /gene="mdoC"
                     /locus_tag="KLH32_003731"
     CDS             complement(2016..3173)
                     /gene="mdoC"
                     /locus_tag="KLH32_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBE0140389.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3567..5102
                     /gene="mdoG"
                     /locus_tag="KLH32_003732"
     CDS             3567..5102
                     /gene="mdoG"
                     /locus_tag="KLH32_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBE0140390.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5095..7638
                     /gene="mdoH"
                     /locus_tag="KLH32_003733"
     CDS             5095..7638
                     /gene="mdoH"
                     /locus_tag="KLH32_003733"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBE0140391.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1998 a         1930 c         2112 g         2007 t
ORIGIN      
        1 accaaaaagc ctgctgtaca cttaagaaac aagaaggtgt aaggaggcgt tatgaaaacg
       61 cgtattcatg ttgtgcaggg tgatattacc aaactggccg ttgatgtgat tgtgaatgcg
      121 gctaatccgt cattaatggg aggcggcggc gtcgatgggg ccattcatcg cgcagcgggt
      181 ccggccctgc tggatgcctg tttaaaagtc aggcaacagc agggcgattg ccctacgggg
      241 catgccgtta ttacgcttgc aggcgatctt cccgctaaag ccgtagtaca caccgtcggg
      301 ccagtctggc gtggtgggga acaaaacgaa gaccagcttt tgcaggatgc ctatctgaat
      361 agcctacgac tggtggcggc aaacagctat acgtcagtgg cttttcctgc aatcagtact
      421 ggggtttata gttaccctcg tgcggcagcg gctgaaatcg cagttaaaac cgtttcagaa
      481 tttattaccc gtcacgcttt acccgaacag gtatactttg tctgttatga tgaagaaaac
      541 gcccacctct acgaaagact ccttacccaa caaggagatg aatgatttgc cccggctggc
      601 gagcgcggtg ctgccactgt gttcgcaaca tcccggtcag tgtggtcttt tccctctgga
      661 gaaaagtctg gatgcgtttg ccgcccggta tcgtctagcc gaaatggcag agcatacgct
      721 ggatgttcag tattacatct ggcaggacga tatgtcgggg cggttactgt tttccgccct
      781 gttagccgca gcaaagcgtg gcgttcgcgt ccgtttgttg ctggacgaca acaatacgcc
      841 cggacttgac gacattttgc gcttgcttga cagtcatcca cgcattgaag tccggctttt
      901 taatcctttc tcgtttcgct tgctacgtcc gcttggatat atcaccgact tttcccgtct
      961 caatcgccgg atgcacaata aaagtttcac tgtcgatggc gtggttaccc tagtgggagg
     1021 acgaaatatt ggtgatgcct attttggtgc aggggaagag ccactttttt cggatttaga
     1081 tgtcatggca ataggccccg tggtagagga tgttgccgat gatttcgccc gctactggta
     1141 ttgcaaatcg gtttcaccct tacagcaggt gctggatgtc ccggaaggtg aaatggcgga
     1201 tcgcatcgag ttacccgcct cctggcataa cgatgccatg acgcatcgtt atttacgcaa
     1261 aatggaatcc agtccattta taaatcatct ggttgatgga acgttgccgc ttatctgggc
     1321 gaagacacgt ttattaagtg atgatccggc gaaaggggag ggcaaggcaa aacggcattc
     1381 actgttaccg cagcgcctgt tcgatatcat gggctcaccc agtgaacgca tcgatattat
     1441 ctcttcctat tttgtaccga cacgcgcagg tgtggcgcaa ctcttacgga tggtgagaaa
     1501 aggggtaaag attgcgatcc taaccaattc tcttgccgct aacgatgttg ctgtcgtcca
     1561 tgccggatac gcgcgctggc gcaaaaaatt gctccgctat ggcgtggaat tatatgaact
     1621 caagccgacg cgtgaacaaa gcagtacgtt acacgatcgc ggcataaccg gtaattccgg
     1681 tgccagcctg catgctaaaa cttttagcat cgatggtaaa acggtgttta tcggctcttt
     1741 caatttcgat ccgcgttcaa cattgctcaa tactgaaatg ggcttcgtga tagagagcga
     1801 aacgctggca cagttaattg ataaacgctt tattcagagc cagtacgatg cggcctggca
     1861 gctccgtctg gacaggtggg gacggatcaa ctgggttgat cgtcatgcaa agaaagaggt
     1921 tattctcaaa aaagaacccg ccaccagttt ctggaagcgg gttatggtca gactggcgtc
     1981 gatattgccc gtggaatggt tattgtaaat gtggcttaac gggctggtgc tttatcgttc
     2041 tcacgcttaa caaccggttt accagagaac aaaaacttca gtaacgggat gcgtaaatga
     2101 atttcataca gaattatcgc aatccctact acgaatatca ggccacagag aaaaccaagc
     2161 cagttggagg tgatgtgcgg tgtaatgtat gcgccgaaaa acagcgttaa cgggtggtga
     2221 accagataga taaacagcga tgcgttaaca aaataagtca cccgcgctga ctggaagtta
     2281 agcaaacggt ggccaaagga gaagaccaca ttcaccatcc acaggccgag gaccatggtg
     2341 atcaccgact cggtttcgta catccaggca tcgccactgc catagcgctg gttgagtaaa
     2401 taagcgacaa acgccaatgc tgcagcgagg gtacagccac gagacggcgt ggtaaacaag
     2461 gctttaagat gagggaaaat gaaagccagt gcgccgagga taaagaacgg taaataaaat
     2521 agcgtttgca tgacaataaa attgaacatg ccattactca gaatgggtgg atacacaata
     2581 aaaatcgttc ttcttattac cgcatagccg atgccgaggc ataaaaaaat caccgatagt
     2641 tttaccatcg agaatttttt attcgtttta tcagaatttt ctaaattatt tctgatgcgc
     2701 ttaaatatcc atacacacag cgtcgtcatg actactaaca ccagtaaaaa ccacaggtgt
     2761 gatattaatt cccaggccaa cgtattatat ttgtcataca atgatagccc aggccaactt
     2821 tccgctttcc ctttgacata ttgcagcata ataaattgcg gtaatgtcag tagggggatg
     2881 gctgttaaca tcgggatacc tacacgttcg acacgtactt tccaccattt tttcaaggga
     2941 tagcgtaaaa aaagcatgta ggaaaagtat ccggatataa cgaaaaatac ctgcatgcgg
     3001 aacgagtgga tgaagtcatt aaaaagggtt agccacaatg acggttcggc gctattcaca
     3061 tgccatgtat ggctcgaata gattaaagaa atatgaaaag ggatccctaa caacatcagc
     3121 caggcgcgga tggagtcgag gaaatattca cgttgcgcgg gtactggatt catatatggt
     3181 taactaatct cggatttttc gtcttatccc tgtcgggtta tgcctttagg cttgttgcca
     3241 tagcgacacc gacctgaccg cgccaggcgc aggcttcaag gtttttatgc atagcatcat
     3301 cgctaccact aaccagaatg gaagcgtctg taagacggtt gataaataaa tttgctggca
     3361 aaccctacac gaagtcgatg cttctgtctt taggagaagc acggaaagtg aaaacggttg
     3421 caatcaggtg cttaatccat gagccagcgt gctgaacgat accgggattc agttgtcgga
     3481 atggctggtt atccattaaa atagatcgga tcgatataag cacacaaagg gggaagtgct
     3541 tactaattat gaaacataaa ctacaaatga tgaaaatgcg ttggttgagt gctgcagtaa
     3601 tgttaaccct gtatacatct tcaagctggg ctttcagtat tgatgatgtc gcaaagcaag
     3661 ctcaatcctt agccgggaaa ggctatgagg cgcctaaaag caacttgccc tccgttttcc
     3721 gcgacatgaa atacgcggac tatcagcaga tccagtttaa tcatgacaaa gcgtactgga
     3781 acaatctgaa gaccccattc aaactcgagt tctaccatca gggtatgtac ttcgataccc
     3841 cggtcaaaat aaatgaagtg actgccaccg cagtcaaacg aatcaaatac agcccggatt
     3901 atttcacttt cggcgatgtt cagcatgaca aagacacggt aaaagacctt ggttttgccg
     3961 gttttaaagt gctttacccg atcaacagca aagataaaaa cgatgaaatc gtcagcatgc
     4021 tcggggccag ctatttccgc gtgattggtg caggtcaggt ttatggcctt tctgcccgcg
     4081 gcctagcaat tgataccgct ttgccatcgg gtgaagaatt tccacgcttc aaagagttct
     4141 ggatcgagcg tccaaaaccg actgataaac gtttaaccat ttatgcattg cttgactcgc
     4201 cgcgtgcgac aggtgcttac aaattcgtgg ttatgccagg gcgtgacacg gttgtggatg
     4261 tgcagtcgaa aatctatctg cgcgataaag tcggcaaact gggggttgca ccgttaacca
     4321 gtatgttcct gtttgggccg aaccaaccgt cgcctgcaaa taactatcgt ccggagttgc
     4381 acgactctaa cggtctgtct atccatgctg gtaatggcga atggatctgg cgtccgttga
     4441 ataacccgaa acatttagcg gtcagcagct tctccatgga aaacccgcga ggctttggtc
     4501 tgttgcaacg aggtcgtgat ttctcccgct ttgaagatct cgatgatcgt tacgatctcc
     4561 gtccaagcgc atgggtgact ccgaaagggg agtggggcaa aggcagcgtt gagctggtgg
     4621 aaattccaac caacgatgaa accaacgata acatcgtcgc ttactggacg ccggatcagc
     4681 tgccggagcc gggtaaagag atgaacttta aatacaccat caccttcagc cgtgatgaag
     4741 acaaactgca tgcgccagat aacgcatggg tgcaacaaac gcgtcgttca acgggggatg
     4801 tgaagcagtc gaacctgatt cgccagcctg acggtactat cgcctttgtg gtcgatttta
     4861 ccggcgcaga gatgaaaaaa ctgccagagg ataccccggt cacagcgcaa accagcattg
     4921 gtgataatgg tgagatagtt gaaagcacgg tgcgctataa cccggttacc aaaggctggc
     4981 gtctggtgat gcgtgtgaaa gtgaaagatg ccaagaaaat cactgaaatg cgtgctgcgc
     5041 tggtgaatgc cgatcagacg ttgagtgaaa cctggagcta ccagttacct gccaatgaat
     5101 aagacaactg agtacattga cgcaatgccc atcgccgcaa gcgagaaagc agcattgccg
     5161 aagactgata tccgcgccgt tcatcaggcg ctggatgccg aacaccgcac ctgggcgcgg
     5221 gaggatgatt ccccgcaagg ctcggtaaag gcgcgtctgg aacaagcctg gccagattca
     5281 cttgctgatg gacagttaat taaagacgac gaagggcgcg atcagcttaa ggcgatgcca
     5341 gaagcaaaac gctcctcgat gtttcccgac ccgtggcgta ccaacccggt aggccgtttc
     5401 tgggatcgcc tgcgtggacg cgatgtcacg ccgcgctatc tggctcgttt gaccaaagaa
     5461 gagcaggaga gcgagcaaaa gtggcgtacc gttggtacca tccgccgtta cattctgttg
     5521 atcctgacgc tcgcgcaaac tgttgtcgcg acctggtata tgaagaccat tcttccttat
     5581 cagggttggg cgctgattaa tcctatggat atggttggtc aggatttgtg ggtttccttt
     5641 atgcagcttc tgccttatat gctgcaaacc ggtatcctga tcctttttgc ggtactgttc
     5701 tgttgggtgt ccgccggatt ctggacggcg ttgatgggct tcctgcaact gcttattggt
     5761 cgcgataaat acagtatatc tgcgtcaaca gttggcgatg aaccattaaa cccggagcat
     5821 cgcacggcgt tgatcatgcc tatctgtaac gaagacgtga accgtgtttt tgctggcttg
     5881 cgtgcaacgt gggaatcagt aaaagccacc gggaatgcca aacactttga tgtctacatt
     5941 ctgagtgaca gttataaccc ggatatctgc gtcgcagagc aaaaagcctg gatggagctt
     6001 atcgctgaag tcggtggcga aggtcagatt ttctatcgcc gccgccgtcg ccgcgtgaag
     6061 cgtaaaagcg gtaatatcga tgacttctgc cgtcgctggg gcagccagta cagctacatg
     6121 gtggtgctgg atgctgactc ggtaatgacc ggtgattgtt tatgcggcct ggtgcgcttg
     6181 atggaagcca acccgaacgc cgggatcatt cagtcgtcgc cgaaagcgtc cggtatggat
     6241 acgctgtatg cgcgctgtca gcagttcgcg acccgcgtgt atgggccact gtttacagcc
     6301 ggtttgcact tctggcaact tggcgagtcg cactactggg gacataacgc gattatccgc
     6361 gtgaaaccat ttatcgagca ctgcgcactg gctccgctgc cgggcgaagg ttcctttgcc
     6421 ggttcaatcc tgtcacatga cttcgtagaa gcggcgttga tgcgccgtgc aggttggggg
     6481 gtctggattg cttacgatct cccgggttct tatgaagaat tgccgcctaa cttgcttgat
     6541 gagctaaaac gtgaccgccg ctggtgccac ggtaacctga tgaacttccg tctgttcctg
     6601 gttaagggta tgcacccggt tcaccgtgcg gtgttcctga cgggcgtgat gtcttatctc
     6661 tccgctccgc tgtggtttat gttcctcgcg ctctctactg cattgcaggt agtgcatgcg
     6721 ttgaccgaac cgcaatactt cctgcaacca cggcagttgt tcccggtgtg gccgcagtgg
     6781 cgtcctgagc tggcgattgc actttttgct tcgaccatgg tgctgttgtt cctgccgaag
     6841 ttattgagca ttttgcttat ctggtgcaaa ggaacgaaag aatacggcgg cttctggcgc
     6901 gttacattat cgttgctgct ggaagtgctg ttttccgtgc tgctggctcc ggtacgcatg
     6961 ctgttccata cggtcttcgt tgtcagcgcg ttccttggct gggaagtggt gtggaattca
     7021 ccgcagcgtg atgatgactc cacttcatgg ggcgaagcgt tcaaacgcca cggctcacag
     7081 ctgctgttgg ggttagtgtg ggctgttggg atggcgtggc tggatctgcg tttcctgttc
     7141 tggctggcac cgattgtctt ctcgttgatc ctgtcaccgt ttgtttcggt gatttccagc
     7201 cgtgccaccg ttggtctgcg caccaaacgc tggaaactgt tcctgatccc ggaagagtat
     7261 tcaccgccgc aggtgctggt tgataccgat cggttccttg agatgaatcg ccaacgctcc
     7321 cttgacgatg gctttatgca cgcagtgttt aacccgtcat ttaacgctct ggcaaccgca
     7381 atggcgacag cgcgtcaccg cgccagtaag gtgctggaaa tcgcccgtga ccgccacgtt
     7441 gaacaggcgc ttaacgagac gccagagaag ctgaatcgcg atcgtcgcct ggtgctgcta
     7501 agcgatccgg tgacgatggc ccgtctgcat ttccgcgtct ggaattcccc tgaaagatat
     7561 tcttcatggg tgagttatta cgaagggata aagctcaatc cactggcatt gcgtaaaccg
     7621 gatgcggctt cgcaataaaa acgtagttgc ctgatgcgct acgcttatca ggcctacatc
     7681 gttcctgcaa tttattgatt ttgcaagatt ttgtaggtcg gataaggcgt tcacgccaca
     7741 tccggcaaaa acaacgagcc aataaaaata ccggcgttat gccgtaatgc cagtcagtta
     7801 agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac
     7861 tctcgatcga taggaattaa aaccccaaaa agattaaaaa aacaccacaa aacggatgtt
     7921 tcttcaacac cacttttgct ccatatgaac ggaaccgacg attaaactgg atggctctga
     7981 ttgattcagg gtatgaatgg cggttttttg ctccgtttcc ctcaaaatgg acgcaacttc
     8041 ccctctg
//
LOCUS       DAEEDS010000138         7918 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEEDS010000138 DAEEDS010000000
VERSION     DAEEDS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.140"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            239..742
                     /gene="dps"
                     /locus_tag="KLH32_003734"
                     /gene_synonym="pexB"
     CDS             239..742
                     /gene="dps"
                     /locus_tag="KLH32_003734"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBE0140392.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1146..1892
                     /gene="glnH"
                     /locus_tag="KLH32_003735"
     CDS             1146..1892
                     /gene="glnH"
                     /locus_tag="KLH32_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBE0140393.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2031..2690
                     /gene="glnP"
                     /locus_tag="KLH32_003736"
     CDS             2031..2690
                     /gene="glnP"
                     /locus_tag="KLH32_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBE0140394.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2687..3409
                     /gene="glnQ"
                     /locus_tag="KLH32_003737"
     CDS             2687..3409
                     /gene="glnQ"
                     /locus_tag="KLH32_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBE0140395.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3670..5894
                     /gene="ybiO"
                     /locus_tag="KLH32_003738"
                     /pseudo
     CDS             3670..5894
                     /gene="ybiO"
                     /locus_tag="KLH32_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="KLH32_003739"
     CDS             complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="KLH32_003739"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBE0140396.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7093..7353
                     /locus_tag="KLH32_003740"
     CDS             7093..7353
                     /locus_tag="KLH32_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0140397.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7618..>7918
                     /locus_tag="KLH32_003741"
     CDS             7618..>7918
                     /locus_tag="KLH32_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE0140398.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1935 a         2026 c         1978 g         1979 t
ORIGIN      
        1 accgtattta tatatcgaat tatgcaaaag catatttatt ccgaaaattc ctggcgagca
       61 gataaataag aattgttctt atcaatatat ctaactcatt gaatctttat tagttttgtt
      121 tttcacgctt gttaccacta ttagtgtgat aggaacagcc agaatagcgg aacacatagc
      181 cggtgctata cttagtctcg ttaattactg ggacataaca tcaagaggat atgaaattat
      241 gagtaccgct aaattagtta aatcaaaagc gaccaatctg ctttataccc gcaacgatgt
      301 ctccgacagc gagaaaaaag caacagtaga gttgctgaat cgccaggtta tccagtttat
      361 tgatctttct ttgattacca aacaagcgca ctggaacatg cgcggcgcta acttcattgc
      421 cgtacatgaa atgctggatg gcttccgcac cgcactgatc gatcatctgg ataccatggc
      481 agaacgtgcc gtgcagctgg gcggtgtagc tctggggacc actcaagtta tcaacagcaa
      541 aaccccgctg aaaagttacc cgctggacat ccacaacgtt caggatcacc tgaaagaact
      601 ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa gcgattggcg aagcgaaaga
      661 tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac ctggataaat tcctgtggtt
      721 tatcgagtct aacatcgaat aaatccatcg ctgatggtgc agaactttag tacccgataa
      781 aagcggcttc ctgacaggag gccgttttgt tttgcagccc acctcaacgc acttatttag
      841 tgcatccatc tgctatcccc agctgattaa gtaaattttt tgtatccaca tcatcacaca
      901 atcgttacat aaagattgtt ttttcatcag gttttacgct aaataatcac tgtgttgagt
      961 gcacaatttt agcgcaccag attggtgccc cagaatggtg catcttcagg gtattgccct
     1021 ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc aatgagttgc aaaattggca
     1081 cgattttttc atatatgtga atgtcacgca ggggatcgtc ccgtggatag aaaaaaggaa
     1141 atgctatgaa gtctgtatta aaagtttcac tggctgcact gaccctggct tttgcggttt
     1201 cttctcatgc cgcggataaa aaattagttg tcgcgacgga taccgccttc gttccgtttg
     1261 aatttaaaca gggcgataaa tatgtgggct ttgacgttga tctgtgggct gccatcgcta
     1321 aagagctgaa gctggattac gaactgaagc cgatggattt cagtgggatc attccggcac
     1381 tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac catcaccgac gagcgtaaaa
     1441 aagcgatcga tttctctgac ggctactaca aaagcggcct gttagtgatg gtgaaagcta
     1501 acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct gtgaagagcg
     1561 gtactggctc cgttgattac gcgaaagcaa acatcaaaac taaagatctg cgtcagttcc
     1621 cgaacatcga taacgcctat atggaactgg gcaccaaccg cgcagacgcc gttctgcacg
     1681 atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc aaagcggtag
     1741 gtgactctct ggaagcgcag caatacggta ttgcgttccc gaaaggtagc gacgagctgc
     1801 gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac aacgaaatct
     1861 acaaaaaatg gttcagtact gaaccgaaat aataacgcta cacctgtaaa gcgcactggc
     1921 agttccctct cccccctggg gagaggatta gggtgagggg cgcaaacccg ctccggggcc
     1981 attaattacc ctgaatttga ttatttacac cacggtaaca ggaacaacat atgcagtttg
     2041 actggagtgc catctggcct gccattccgc ttctgattga aggtgccaaa atgaccctgt
     2101 ggatttcggt cctcggtctg gctggcggtc tggtaatcgg attgctggca ggttttgcac
     2161 gcaccttcgg tggttggata gccaaccacg tcgcgctggt ctttattgaa gtgatccgcg
     2221 gcacacctat cgtcgtccag gtgatgttta tctatttcgc cctgccgatg gcgtttaacg
     2281 acttacgcat cgacccattt actgcggcgg tggtcaccat catgatcaac tccggcgcgt
     2341 acattgcgga aatcacgcgt ggtgcggtgc tgtctatcca caaaggtttt cgtgaagcag
     2401 gactggcgct cggtctttca cgttgggaaa ccattcgcta cgtcatttta ccgctggcac
     2461 tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat cagcattaaa gacacctcgc
     2521 tgtttattgt tatcggcgtg gcggaactga cccgtcaggg gcaagaaatt attgccggta
     2581 acttccgcgc ccttgagatc tggagcgccg tggcggtgtt ctatctgatt attaccctgg
     2641 tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa aatcctgtga ttgaatttaa
     2701 aaacgtctcc aagcactttg gcccaaccca ggtgctgcac aatatcgatt tgaacattgc
     2761 ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga ccctgctgcg
     2821 ctgcatcaac aaactggaag aaatcacctc cggcgatctg attgtcgatg gcctgaaggt
     2881 caacgatccg aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg tgttccagca
     2941 gttttacctc ttcccgcatc tgacggcgct gggaaacgtc atgtttggtc cgctacgcgt
     3001 gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt gagctgctgg cgaaagtcgg
     3061 tctggcagaa cgtgcacatc actacccttc cgaactttct ggtggtcaac agcagcgtgt
     3121 ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg aaccgacttc
     3181 cgctcttgac ccggaactgc gccatgaagt gctgaaggtt atgcaggatc tggctgaaga
     3241 agggatgacg atggtgatcg tgacccacga aatcggtttt gccgagaaag tagcttcgcg
     3301 gctgatcttt atcgacaaag gccggattgc ggaagatggc aatccgcagg tgttgatcaa
     3361 gaacccgccg agccagcgct tgcaggaatt tttgcagcac gtctcttaat aagacacatt
     3421 gcctgatgcg ctacgcttat caggcctaca ggatatctgg caacttatta aaattgcatg
     3481 aacttgtagg acggataagg cgttcacgcc gcatccggca aaaaagcccg cacgttgtca
     3541 gcaacctgct taatatccct tcctcccttt cacccgaaag ggaggcacac cagattcctc
     3601 tcatttaaaa tcgcccctcc cccagcatct atacttatct ttttgctctg ttttctcact
     3661 ggaggagtca tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc acctgcccac
     3721 gcggtatcca tacccggcgt tacaaccaca acgacaacgg actcaacgac tgaaccggcc
     3781 ccggaaccgg atatcgaaca aaaaaagcgg cctatggcgc actggcggat gtgctggata
     3841 acgacacctc gcgtaaagag ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg
     3901 cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa
     3961 aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt
     4021 atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc
     4081 tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt
     4141 gcgcactgcc gctatatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca
     4201 gcaactggtt gcagcttccg gcgatgatta ttggggcgtt tattatcgac ctgctgttac
     4261 tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca
     4321 ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag
     4381 ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg
     4441 acgagagcgc ccgttactgg ggtcgtcgcc tgagctggtt aagcagcctg attggctatg
     4501 gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg
     4561 cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc ttccgaaata
     4621 aaaaagagat tacccagcat atgctcaact ttgcggagca ttcgctggct tttttcagcc
     4681 tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcatgc
     4741 tgtttttctt ttcgttgttc gatccgggca acagtctgaa atttatgatg ggtgcaacgg
     4801 tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc
     4861 tggcgaaaac catcaccctc tcgccacata ctcagcgtaa ctatccggag ctacaaaaac
     4921 ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg
     4981 cagtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg
     5041 gcgcggggca gaaaaccgta gatatcctga tccgcatcgc actcattctt ttcttctcgg
     5101 cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg
     5161 gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg
     5221 cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg
     5281 cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc
     5341 tggtaaaaga tattatcacc ggggtattta ttcagtttga aaacggcatg aacactggag
     5401 atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg
     5461 gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg
     5521 ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag
     5581 atgctgataa agccaatcag gcactgaaag atgcggtaac ggaattgatg gaaaacgaag
     5641 aaattcgcgg gctgattatt ggtgaaccga actttgccgg gattgtcggc ttaagcaata
     5701 ccgcgtttac actgcatgtt tcgttcacca cgctgccact caaacagtgg acggtacgct
     5761 ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc
     5821 agacttatca ggtgctgcct gctccgggcg cgaccccggc tgaaccgctg ccgccggggg
     5881 agccaacgct ttaacgttgg cgattaacaa aacggcggcg ctgctcgtcg ttcataaagg
     5941 tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca
     6001 ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca
     6061 gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga
     6121 taaagttgac ttcaccgcct tcacaccaca actcctgttg ctgaccacca aagttcagtg
     6181 catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgccgcag
     6241 cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttgtgga
     6301 tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa
     6361 gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac
     6421 taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac
     6481 caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta
     6541 aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt
     6601 cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct
     6661 tcaccgccag cggattggca aagtctacgc tttgctcccc tgcgggtgta agcgtgagga
     6721 attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac
     6781 ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc
     6841 gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa
     6901 ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt
     6961 gatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcatatctc tgtcaatttt
     7021 ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat
     7081 gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc
     7141 tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc
     7201 gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca
     7261 gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac
     7321 cagatgttag gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc
     7381 tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaacg caatgtaaat
     7441 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg catcaagtga taatgcttat
     7501 caaaattact atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca
     7561 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
     7621 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
     7681 tgtattggta ttacgcctgt ggcacaggca ctcgccgccg aagggcaagc taacgcggat
     7741 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
     7801 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa
     7861 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgt ccccggcg
//
LOCUS       DAEEDS010000139         7887 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEEDS010000139 DAEEDS010000000
VERSION     DAEEDS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.141"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="KLH32_003742"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="KLH32_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KLH32_003743"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KLH32_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBE0140399.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KLH32_003744"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KLH32_003744"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBE0140400.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KLH32_003745"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KLH32_003745"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBE0140401.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="KLH32_003746"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="KLH32_003746"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBE0140402.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1793 a         2050 c         2127 g         1917 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga aagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta
     7861 gagcacgacc ttgccaaggt cggggtc
//
LOCUS       DAEEDS010000140         7746 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEEDS010000140 DAEEDS010000000
VERSION     DAEEDS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.142"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1016
                     /gene="ydfJ"
                     /locus_tag="KLH32_003747"
     CDS             <1..1016
                     /gene="ydfJ"
                     /locus_tag="KLH32_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HBE0140403.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1105..2565
                     /locus_tag="KLH32_003748"
     CDS             1105..2565
                     /locus_tag="KLH32_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE0140404.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="KLH32_003749"
     CDS             complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="KLH32_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBE0140405.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2982..3668)
                     /gene="ydfH"
                     /locus_tag="KLH32_003750"
     CDS             complement(2982..3668)
                     /gene="ydfH"
                     /locus_tag="KLH32_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBE0140406.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="KLH32_003751"
     CDS             complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="KLH32_003751"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBE0140407.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4640..6685
                     /gene="dcp"
                     /locus_tag="KLH32_003752"
     CDS             4640..6685
                     /gene="dcp"
                     /locus_tag="KLH32_003752"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBE0140408.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6730..7248)
                     /locus_tag="KLH32_003753"
     CDS             complement(6730..7248)
                     /locus_tag="KLH32_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBE0140409.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7524..>7746
                     /gene="ydeI"
                     /locus_tag="KLH32_003754"
     CDS             7524..>7746
                     /gene="ydeI"
                     /locus_tag="KLH32_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBE0140410.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1942 a         1814 c         1954 g         2036 t
ORIGIN      
        1 ccatcttgct ggtgacgctg cgtattattc agggattggg tgcaggtgcg gaaatttccg
       61 gtgccggtac gatgctggcg gaatatgcgc caaaaggtaa gcgcggaatt atctcctcat
      121 ttgtggctat gggaactaac tgcggcacct taagcgcaac ggcaatctgg gcctttatgt
      181 tcttcattct cagtaaagag gaactgctgg cgtggggatg gcgtataccg ttcctagcga
      241 gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa tctgaaagaa agcccggtct
      301 ttgagaaggt taacgacagc aaccaaccga cagcaaaacc tgcacctgct ggtagcatgt
      361 tccagagcaa atccttctgg ctggcaacag ggctgcgttt tggtcaggcg ggtaactccg
      421 gtttaattca gactttcctt gcaggctatt tagtacagac gttattgttt aacaaagcaa
      481 ttccaacaga tgcattgatg atcagttcga ttctcggctt tatgaccatt ccgttccttg
      541 gttggttatc cgataaaatt ggtcgccgga tcccgtatat tattatgaat acctctgcga
      601 ttgtgctggc gtggccaatg ctttctatca tcgtagataa aagctatgcc ccgagcacca
      661 ttatggttgc actgattgtg attcataact gtgcggtgct gggattattt gctctggaaa
      721 acattaccat ggcagaaatg ttcggctgta aaaaccgctt cacccggatg gctatttcaa
      781 aagaaattgg tggtcttatc gcttccggtt ttggtcctat cctggcgggt attttctgca
      841 ccatgacgga atcctggtat ccgatcgcca ttatgatcat ggcatattca gtgattggtt
      901 taatctctgc gctgaaaatg ccagaggtga aagaccgtga tttaagtgcg ctggaagacg
      961 ctgcggaaga tcaaccgcgt gttgtaagag ctgcgcaacc ttccagaagt ctgtaaaccc
     1021 tcaatccctt ctcttaccgg agaggggatt tttattcata taacaaaaca tatagcttgc
     1081 catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa agcgacactc
     1141 cctgtttatg atcgtaataa cctggcccca agaattgttc atttaggctt tggtgcattt
     1201 caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt cagtgactgg
     1261 ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt acaacagcaa
     1321 gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg cgtcgttggc
     1381 gtcgttaaaa aagccttgca cgtacagatg gatggcttag aaaccgtgtt ggctgcgatg
     1441 tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta tttccactct
     1501 ccggcgaccg gacagttaat gctcgagcac ccgatggtcg ctgccgacgt gcaaaatccc
     1561 caccagccga aaacagcaac aggggtgatt gttgaggcgc tggctcgccg taaagcggca
     1621 ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg tcatgttatg
     1681 cgtgacgttg tcacttccta cgcgcaagcc gttgatgtaa aactggcaca atggatcgaa
     1741 gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt gacagaggat
     1801 acgctggcga aaatcgaaca acttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa
     1861 cctttccgcc agtgggttat cgaagataac tttgtagccg gacgtccgga atgggaaaaa
     1921 gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc
     1981 aacggcagtc attcattcct ggcatatctg ggttatcttg ctggatatca gcacattaat
     2041 gactgtatgg aagatgaaca ttatcgtcat gcggcgtatg gcatgatgtt gcaggaacaa
     2101 gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg attaattgca
     2161 cgctatagca acccggcgtt acgtcatcga acctggcaga ttgcgatgga tggcagccag
     2221 aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga cagcaagttc
     2281 gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag
     2341 ggaaatccga tagaaatcag tgacccactg ttacctgtta ttcagaaggc tgtacaaagt
     2401 agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcaat ctttggtgat
     2461 gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt gtctttatta
     2521 gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc
     2581 catcggcggc attcacacac ttagttcatg ccgagacgaa tcaggtccag aggtgccagt
     2641 ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc agcggtcaga
     2701 ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac cataacacgg
     2761 ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt tttctccttt
     2821 ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt cgagtgaaat
     2881 attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc gtaaggcggg
     2941 gggaaaagcc tctcatcccg gatgggatga gagtaaatga attactcttc gctaaaccag
     3001 tcgctgtttt cctggcggat ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc
     3061 attgcacgtt ctaccgcatc gccatcacgt ttttgcaggg cagagaaaat atcaagatgc
     3121 tggcgtaaca gcatttctgg tggagaaaca tggtcgaaac tcatatagcg cacgcgatca
     3181 acggtcgctt tcaggttctc aatggtatcc cacgccagtt gacagtcggc aatctgcgtc
     3241 aggagttgat ggaagttgtc atcaagttca aaaaaatcat ccagttgctt gcgctcaatg
     3301 gcaatgcgtt gctggtgaag attttgttcc agttggtagc actggctttc ggtaatcatg
     3361 ctcgccgccc gacgcgccac cgcgcactca atggcctgac ggataaaact gccgttgcgc
     3421 acctgggcca tggaaatttt gttgacgtag ctgccacgct gcggacgaat ttgaatcagg
     3481 ccgttttccg ccagtttaat aaaggcttca cgaaccggct ggcgtgacac attgaaacga
     3541 acagaaactt ctttttccga caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg
     3601 cgacgaagaa tacgataaat ctgctgattg acaggctgtg tgggattaag ttgcgtttcg
     3661 acggtcattc gttcttgctt gtgagtgagt taactgcatg agtctaccac ttttttgtga
     3721 caagcaataa cccggcagtt acgccgggta taaaaattac tgacggtgga cattcagtcc
     3781 ggcatagctt tgggtaaccg gcatcatttc cagggtattg atattgacgt gagcaggcag
     3841 cgttgacacc caccagacgg cttcgctgac atcttctggc gtcaatgcaa cggtattttg
     3901 ataggttttt tccgctttac cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc
     3961 acccaccaga cccggttcga tgtcggtgac gcgcaccgcc gtaccatgca gatccgtacg
     4021 cagattcagg ctaaactgac gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc
     4081 atacggccag ctgcctgccg ttgagccaat gttaataata tgaccatgat tacgttcaac
     4141 catacccggt aagacggcgc gcgtcatata tactaggcct ttgttgttgg tatcaatcat
     4201 cgtttcccag tcttcaacgc tggctttatg cgcaggctcc atgcccaacg ccaggccggc
     4261 attatttacc aggatatcaa tattgctcca ctcggcaggg agcgatgcca gcatctcttc
     4321 aatagcggcg cggttgcgaa cgtccagttg ggcgatatac agattatctc ccagttcgtc
     4381 tgttaactcc tgcaaccgct cctggcgacg gccagtggcg ataactttat gcccttgttg
     4441 aataaaacga cgagtaatgc attcaccaaa acctgccgtt gctccagtta ctaaaacgat
     4501 catctcactg ttcctcaacg cttttgtgtg tcaataacat agcacgtgat gcttaagagc
     4561 gggtaatact ggtttgcaag attgcactcc gttatcagta agcctacgct atggaacacc
     4621 tcgcgttcag gagaagaaaa tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc
     4681 gtatctggct cctcattttg atcaaattgc caatcatcac tatcgcccgg cattcgatga
     4741 gggaatgcaa caaaagcggg cagaaattgc tgccatcgcg cttaacccgc aaacgcctga
     4801 tttcaacaat actattctgg cactggaaca aagcggagaa ttaattaccc gcgttaccag
     4861 cgtctttttt gcgatgactg cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca
     4921 gttttccgct gaactggcgg aactggctaa tgatatctat ctgaacggtg aattattcgc
     4981 gcgggtagat gctgtctggc agctccgtga atccctgggg cttgatagtg aatccatccg
     5041 cctggtggag gttattcatc aacgttttgt ccttgccgga gccaaacttg cacaagctga
     5101 taaagcaaaa ttaaaagtac tgaatacaga agctgcgacc ctgaccagcc agtttaacca
     5161 gcggttactt gcagcaaata aatccggcgg tctggttgtg aacgatatcg cgcagctggc
     5221 aggaatgagt gagcaagaga ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga
     5281 taacaaatgg ctgattccgc tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg
     5341 cgatcgtgcg acgcgtgaaa aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga
     5401 tgccaatgat acccgcgcta tcattcaacg tctggtagag attcgcgcac agcaggcgaa
     5461 actgcttggt tttcctcatt atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc
     5521 agaagcagca ctcaacttta tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga
     5581 tgaattagcc tccatacagg cggttatcga taagcagcag ggcgggttta gcgcgcagcc
     5641 gtgggactgg gcattttatg cggaacaggt acggcgggag aaatttgatc ttgatgaggc
     5701 gcagctcaag ccatattttg aattaaacac ggtgttaaat gaaggtgtat tctggaccgc
     5761 gaatcagctc ttcggtatta agtttgtcga acgttttgat attccggtct accatcctga
     5821 cgttcgtgtg tgggaaattt ttgatcataa tggcgtggga ctggcgttat tttacggtga
     5881 tttcttcgcc cgtgattcaa aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc
     5941 aacgcttaat gaaacgcatc cggtaattta taacgtctgc aattatcaga aacccgctgc
     6001 cggtgagcct gcgttgttac tctgggatga tgtaataacc ttattccatg aatttggtca
     6061 tacgctgcac ggcctttttg cccgccagcg ttatgccacg ctttccggca ccaacacgcc
     6121 gcgtgatttt gtcgaatttc cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt
     6181 attcgctcgc tacgcccggc attatcagag cggggcagca atgcctgacg aactgcaaca
     6241 gaaaatgcgt aatgccagcc tgttcaacaa agggtatgag atgagcgaac tgcttagcgc
     6301 cgcacttctc gatatgcgct ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga
     6361 tgattttgaa ttgcgggcgc tggtggcgga aaatatggat cttcctgcta taccgccacg
     6421 ctatcgcagc agttatttcg cccatatttt tggtggcgga tatgctgcag gttattacgc
     6481 ttatctgtgg acgcaaatgt tggccgatga tggttaccag tggtttgttg agcagggcgg
     6541 attaacgcgt gaaaatgggc agcgttttcg cgaggcgatc ctttccagag gtaacagcga
     6601 agatctggaa cgcctgcatc gacaatggcg tggtaaggct cctcagatta tgccgatgct
     6661 gcaacatcgt ggcttgaaca tataaacacc accaatggct ggcctggaga tatcgccagg
     6721 ccagcctggt tatagcagta attgcagcag ctgggcgagg gcaaacctca ccgctaaagc
     6781 taataccgtt tcgcagtcgc cagcaaaatg cataaccgca gtgtagttct ggcctttaat
     6841 atgccacgcg aaccagacgg tacccgctgg cgtaccatct tcaccgccct ccggtccgcc
     6901 gtagccggta atggcaatac tgacatcagc atccgcacgc tctatggcac ctgttgccat
     6961 ttctgctgcc actttctcac tcaccgcaga atatcgttca agagactgct ggcttacgct
     7021 gaggattttc atctttgcct gatcggtgaa agtaacaaag cctgcaccgt aaaatttggg
     7081 tgtatcttca gctgcacaca gggcgctagc caacttaccg ccggtgcatg attcggcggt
     7141 agttaagcgt aattgatccg cgataagtct ttgactcaac gcggatgtca ggttttcaat
     7201 agtgtcggta tctgcgagtt gtacaatttt atctttatta atgttcactg ttttttgtcc
     7261 acgctattat atgtaaaggt gatttgcttt ttctgtatgc tcgtccaata taataatttt
     7321 caaacatata aatccgatta ttctgaaaac atgtttaaga ctcttcaatc ttcttaactt
     7381 tagtgagtca aattctctga atatttgaca gctagtgggc tgattttaaa tgccttataa
     7441 caattggcag tattcttatt tggcaattat aaaaatagca accagactta ataagtcaaa
     7501 ccaaacgcaa ttaaggagtt agtatgaaat ttcagtccat tgtattagca agttttcttg
     7561 tcatgcctta tgcgttggca gatgaccagg gcggtttaaa acaggatgca gcgccaccgc
     7621 cgcctcatgc aatagaagat ggctatcgcg gaaccgatga tgcaaaaaaa atgaccgttg
     7681 atttcgcaaa aaacatgcac gatggcgctt cggtttcact acgcggtaat ttgatttctc
     7741 acaaag
//
LOCUS       DAEEDS010000141         7726 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEEDS010000141 DAEEDS010000000
VERSION     DAEEDS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.143"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..835)
                     /locus_tag="KLH32_003755"
     CDS             complement(<1..835)
                     /locus_tag="KLH32_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HBE0140411.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQ"
     gene            1017..2441
                     /gene="sbcB"
                     /locus_tag="KLH32_003756"
     CDS             1017..2441
                     /gene="sbcB"
                     /locus_tag="KLH32_003756"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBE0140412.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2484..2711)
                     /locus_tag="KLH32_003757"
     CDS             complement(2484..2711)
                     /locus_tag="KLH32_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBE0140413.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="KLH32_003758"
     CDS             complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="KLH32_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBE0140414.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3962..5320)
                     /gene="plaP"
                     /locus_tag="KLH32_003759"
     CDS             complement(3962..5320)
                     /gene="plaP"
                     /locus_tag="KLH32_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBE0140415.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5310..5372)
                     /gene="yoeI"
                     /locus_tag="KLH32_003760"
     CDS             complement(5310..5372)
                     /gene="yoeI"
                     /locus_tag="KLH32_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBE0140416.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5533..6516)
                     /locus_tag="KLH32_003761"
     CDS             complement(5533..6516)
                     /locus_tag="KLH32_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0140417.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6562..7386)
                     /locus_tag="KLH32_003762"
     CDS             complement(6562..7386)
                     /locus_tag="KLH32_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0140418.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7469..7723)
                     /gene="yoeB"
                     /locus_tag="KLH32_003763"
     CDS             complement(7469..7723)
                     /gene="yoeB"
                     /locus_tag="KLH32_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBE0140419.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1812 g         1833 t
ORIGIN      
        1 tttgttcgaa gattggacgg cggataacat tacccatctt gctatgctct ttcggtacgg
       61 tccatacgtt gtccagcagg tcaaattctg tctttgttgc cagcctaagc tctgagagcc
      121 tcgcccccca cagcataagc atctgatgaa ggagcttatt tgacgtagac gcacggcttc
      181 tttcaatagc aagccaaatc ttagccagtt cgtgatacga cagtacccga tcccctacct
      241 cagcgcggga gccgaagtcc cttggctgga tgctcataat tgcgcaacta tctatcaact
      301 gacgccgcat gcaccaacta attgctgatc ttagttgact tagcacctgc cttgctcggc
      361 gtggattatc tttttcttct tcggtaagca ggtcaaccca ttgcttaacc gtgatagaag
      421 atgccggacg attagggaag gcgtcatgca tgcgcttcat aaccgttgat cgataaagtg
      481 cctgggtctt ttctctgaga gttgtagaga cgtagttgtc gaaccagtag tcgagacact
      541 gggcgaccgt catggagttc tccaccttct cttcaaaata ggtgcgtgga tccgttcctg
      601 agaaatagag ctttcgcaag tcagcagtga tctgtctggc atccttcaaa gacagggatg
      661 ggtatcgacc aagcccaagt cgattaggct tgccatgcca acgatagcgg tactggaact
      721 ggatgacccc cttcggtgaa attcgtacgc tgaggccatc ggcatcagcc acttcttgtg
      781 ggcccgaata tggtttacca taaatagtac gcagttttgt gtcgcttata gccataaata
      841 atatttggta cgctcagaaa taatagtttg gcacacatct ggtacacaat atcacatgga
      901 caacagcgca cagtaaacaa ctatatttga cgttcgtaga catactcagg tgaaaaaagg
      961 gttgcttttc gagttataac agacagttaa ttaactacag tggacaatgc tagacaatga
     1021 cagacacaga taaacaacct accttcctct ttcacgatta cgaaaccttt ggcacgcacc
     1081 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca
     1141 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cggcctggag
     1201 cagtattaat taccggtatt accccgcagg aggcacgggc gaaaggagaa aacgaagccg
     1261 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
     1321 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
     1381 cttacgcctg gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg
     1441 cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga
     1501 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
     1561 cgatggctga tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac
     1621 gcctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg
     1681 ttccgcagat gaaacccctg gctcacgttt ccggaatgtt tggggcatgg cgtggcaata
     1741 ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggcgg
     1801 atttggcagg agacatttcg ccattactgg agctggatat cgacacattg cgcgagcgtt
     1861 tatataccgc aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata
     1921 tcaataaatg tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac
     1981 tgggaattaa tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag
     2041 tgcgtgaaaa agtggtagcg atattcgcgg aagccgaacc gtttacacct tcagataatg
     2101 tggatgcaca gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg
     2161 tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa
     2221 ttgaaaagtt gttgttcaat tatcgggcac gcaacttccc ggggacgctg gattatgccg
     2281 agcagcaacg ctggctggag caccgccacc aggtcttcac gccagagttt ttgcagggtt
     2341 atgctgatga attgcagatg ctggtacaac aatatgccga tgacaaagag aaagtggcgc
     2401 tgttaaaagc actttggcag tacgcggaag agatcgtcta atttgaagcc gccgccgctg
     2461 gtacggcgga ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg
     2521 ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct
     2581 tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc
     2641 agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt
     2701 ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg
     2761 ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag
     2821 gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac cgatagagca
     2881 accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt
     2941 tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatgc
     3001 ctccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc
     3061 gaccagaaat tgaagaatat tggccgttgg ggaagtgact cctaacccaa acatgcgtcc
     3121 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
     3181 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
     3241 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
     3301 cagcaaaacg gtaaccaacg gccacacaga caaattaaaa gtctcagcaa tagagttatg
     3361 ttcagtagtg tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac
     3421 cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
     3481 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaaaagata
     3541 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
     3601 ctgaattaac gcaaaaaccc ccacgctttg tgccgaaatt gcaatcagca aggcgtaaaa
     3661 catgcgatta tttttcgcga tatacatatc gcgaaaacca ccagtcaggc aaaaacgccc
     3721 acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaaa
     3781 cataaattaa ttaaccagat gaatgttaat ggggaaattt ttcatgactg gtggaatgca
     3841 gaccaataac caaattctct aaattagaac aaatggttat taatgaggat ttcgataaaa
     3901 aagaataaaa aaaccggagc caaatgttca ttcgactccg gtttattatt agaaaagatg
     3961 attatgctac gtcttcgtac tgcggaaccg gattacggaa gctcttggtg acgcaagcca
     4021 ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt tcttccaggt
     4081 taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt
     4141 gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg accgacaggt
     4201 taacaaaggt gaacgccacc agcgcaccaa agttaatcag cgccgttgcc attaccaggt
     4261 cgaagttgat tgccagcagg gcaatcgcgc caaccaggat gatgttcatc gccggagtac
     4321 gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacgccgtca cgacccatta
     4381 cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg
     4441 tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc atgatttcag
     4501 gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt
     4561 aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc gcacgcggga
     4621 tcacgcgctc tgcatctttg gtttcttccg acagattgct gatgccgtca aagccggtaa
     4681 aggagaagca caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc
     4741 agaacggacg cgtgctcgcc agcgtaccag cgccttcacc ttcaaatacg ccataaacaa
     4801 ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgacgt
     4861 tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc agcgccacca
     4921 caaacatcca cgatggaatg gaaggcacca gagcttcaaa atagattttc gccagcagaa
     4981 tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga
     5041 cagtcgggct aatggatttc tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc
     5101 gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg
     5161 ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaatgtatca aacagcgtca
     5221 tcggctgcat ataggctaga cccatcatta caaccggaac taacgtaagc gttttacgca
     5281 attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggttattctc ctttacggtg
     5341 ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact
     5401 gtcgattttt agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg
     5461 tttgaagcaa aaaataaccg acgctaatta aacgtcggtt tttaatcatt ttttgacagc
     5521 ggcgcattgt gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat
     5581 ttttctttga atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact
     5641 atttgctaaa atttcatctc gccaccagcc gctggcaagc cctgcggtct gttcattcca
     5701 gccaatccag acaggttcaa agagagtttg ggcgaccact ttcttctcga ttaacgcgcc
     5761 actatcgaga aaacgttgcg ccagataccg tggtaaatag ccgcagccaa ggccgctaat
     5821 ttgcagctcc agtttggttt taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag
     5881 ttgtgaggcc gtagaagcgc ctgcctgcgc agtgtccccc accacaatcg cccgatatcg
     5941 tttaatgata cgacggttta acggttcttc ttcctgggct aacggatgat gaggcgcgac
     6001 cgcaaagact tgttcaagat cgcccaaccg cgagaaacca aactcactgg aagatggtgg
     6061 ctcatgcatc gcgccaacga taatatccgc tcgcccctga gtcagggcat cccaggaacc
     6121 accgagtacg ccattgataa acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc
     6181 aatgagaggc gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt
     6241 ttcccagcct tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac
     6301 ttcccgccct ttctctaata acatttttcc ggtgcgggtg aatttagcgc ggtgtccgct
     6361 acgatcaagc aattgaatat ttaggtcgct ttccagcttg tgaacggtgt aactcagagc
     6421 ggacggcgtc ttgtagagtt tggctgacgc cgccgcaaaa ctgccctctt tttctatggc
     6481 atcgaggatc atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgcatgcg
     6541 gcgtcctgct ggcgtgatcg tttactccaa cggcattacc agcggatcgg gatactggta
     6601 ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc
     6661 cagactattt ctgaactgcg gaggttgcag ccccagtaaa cgggccatct gcggatagaa
     6721 aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc
     6781 ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg
     6841 ttcaccatca ggggcggttt ttccggcaaa gaagcgtccc ggatgacgtc ccggtccgac
     6901 caggcccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc
     6961 gagttcttct aatactcgtc cactgttggt taccggatta cgcggggtag tttctttaac
     7021 cgtgccttgc gcgtcgccat agacagatgt tgagctggta aaaataattc ggggaatacg
     7081 atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg
     7141 gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg gcatccataa gggcatccag
     7201 atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact
     7261 cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg
     7321 cgctgaaagt gacatcgcca gcggcatacc taaccaccct aacccgacaa ttgcgacctt
     7381 tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac
     7441 tgacaattca ttctatgaat gaatctgttc aataatgata acgacatgct gcaatgagca
     7501 gtgaatcatc agtaaccgcg tataccagac ggtgctcctc tgtaatgcgt cgggaccaga
     7561 aacctgacaa attatgtttc aggggttctg gtttcccctt accttcaaat ggtgttctgc
     7621 gggtatcttt gataagttca ttgatctttt taacaattcg cttatctgtt tcctgccagt
     7681 acagataatc atcccatgat tcctcagacc agattagttt cactca
//
LOCUS       DAEEDS010000142         7557 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEEDS010000142 DAEEDS010000000
VERSION     DAEEDS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.144"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..1161
                     /gene="rbsA"
                     /locus_tag="KLH32_003764"
                     /pseudo
     CDS             1..1161
                     /gene="rbsA"
                     /locus_tag="KLH32_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1166..2131
                     /gene="rbsC"
                     /locus_tag="KLH32_003765"
     CDS             1166..2131
                     /gene="rbsC"
                     /locus_tag="KLH32_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBE0140420.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2156..3046
                     /gene="rbsB"
                     /locus_tag="KLH32_003766"
                     /pseudo
     CDS             2156..3046
                     /gene="rbsB"
                     /locus_tag="KLH32_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3172..4101
                     /gene="rbsK"
                     /locus_tag="KLH32_003767"
     CDS             3172..4101
                     /gene="rbsK"
                     /locus_tag="KLH32_003767"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBE0140421.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4105..5097
                     /gene="rbsR"
                     /locus_tag="KLH32_003768"
     CDS             4105..5097
                     /gene="rbsR"
                     /locus_tag="KLH32_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBE0140422.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5063..6490)
                     /gene="mdtD"
                     /locus_tag="KLH32_003769"
     CDS             complement(5063..6490)
                     /gene="mdtD"
                     /locus_tag="KLH32_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBE0140423.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6513..7205)
                     /locus_tag="KLH32_003770"
     CDS             complement(6513..7205)
                     /locus_tag="KLH32_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBE0140424.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1924 a         1828 c         1988 g         1817 t
ORIGIN      
        1 gaaaaccatg tatgtcgaag cggataaatt gctggctaaa cttaacctgc gctttaaaag
       61 cgacaagtta gtgggcgatc tttccatcgg tgaccagcaa atggttgaaa tcgccaaagt
      121 gctgagcttt gagtcgaaag tcatcattat ggatgaaccg accgatgcgc tgaccgatac
      181 cgaaaccgaa tccctgttcc gcgtcatccg cgagctgaaa tcgcaaggcc actgtattgt
      241 ctatatctcc caccgcatga aagaaatctt cgagatttgc gatgacgtta ccgtttttcg
      301 tgatgggcaa tttattgctg agcgcgaagt ggcatcactg accgaagatt cgctgattga
      361 gatgatggtg ggccgcaagc tggaagatca atatccacac ctggacaaag cgccaggaga
      421 tatccgcctg aaagtcgata atctctgcgg acctggcgtt aacgatgtct cttttacttt
      481 acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc gcaggtcgta ccgaactgat
      541 gaaagtgctc tacggcgcac tgccgcgcac cagcggttac gtcaccctgg atgggcatga
      601 agtcgttacc cgttcaccgc aggatggcct ggcaaacggc attgtgtata tctccgaaga
      661 ccgtaaacgt gacggtttag tgctgggcat gtcagtaaaa gagaacatgt cgctgacagc
      721 gctgcgctac ttcagccgcg ctggcggcag tttgaagcat gccgatgaac agcaggctgt
      781 gagtgatttc attcgtctgt ttaatgtgaa aacgccgtcg atggaacagg caattggtct
      841 gctttccggt ggcaatcagc aaaaagtggc gattgcccgc ggtctgatga cacccccaaa
      901 gtattgatcc ttgatgagcc tacccgtggc gtagatgtcg gcgcgaaaaa agagatctat
      961 caactgatta accagttcaa agccgatggc ttgagtatca ttctggtgtc atcggagatg
     1021 ccagaagtat taggcatgag cgatcgcatc atcgtcatgc atgaagggca tctcagcggg
     1081 gaatttactc gtgagcaggc cacccaggaa gtgttaatgg ctgtcgctgt gggcaagctt
     1141 aatcgcgtga atcaggagta aaaaaatgat aacccagact gtctctggtc gccgttattt
     1201 cacgaaagcg tggctgatgg agcagaaatc gcttatcgct ctgctggtgc tgatcgcgat
     1261 tgtctcgacg ttaagcccga actttttcac catcaataac ttattcaata ttctccagca
     1321 aacttcggtg aacgccatta tggcggtcgg gatgacgctg gtgatcctga cgtcgggcat
     1381 agacttgtcg gtaggttctc tgttggcgct aaccggcgca gttgctgcat ctatcgtcgg
     1441 cattgaagtc aatgcgctgg tggctgtcgc tgctgctctc acgttaggtg ccgcaattgg
     1501 tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag gcgtttatcg ctacgctggt
     1561 tatgatgctt ttactgcgcg gcgtgactat ggtttatacc aacggtagcc cagtgaatac
     1621 cggctttact gagaacgccg atctgtttgg ctggtttggt attggtcgtc cgctgggcgt
     1681 accgacgcca gtctggatca tggggattgt cttcctcgcg gcctggtaca tgctgcatca
     1741 cacgcgtctg gggcgttaca tctacgcgct gggcggcaac gaagcggcaa cgcgtctttc
     1801 tggtatcaac gtcaataaaa tcaaaatcat cgtctattct ctttgtggtc tgctggcatc
     1861 gctggccggg atcattgaag tggcgcgtct ctcctctgca caacccacgg cggggactgg
     1921 ctatgagctg gatgctattg ctgcggtggt tctgggcggt acgagtctgg cgggcggaaa
     1981 aggtcgcatt gttgggacgt tgatcggcgc attaattctt ggcttcctta ataatggatt
     2041 gaatttgtta ggtgtttcct cctattacca gatgatcgtc aaagcggtgg tgattttgct
     2101 ggcggtgctg gtagacaaca aaaagcagta ataacgacta caggacatct tgaatatgaa
     2161 catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta agcgccaccg tcagtgcgaa
     2221 tgcgatggca aaagacacca tcgcgctggt ggtctccacg cttaacaacc cgttctttgt
     2281 atcgctgaaa gatggcgcgc agaaagaggc ggataaactt ggctataacc tggtggtgct
     2341 ggactcccag aacaacccgg cgaaagagct ggcgaacgtg caggacttaa ccgttcgcgg
     2401 caccaaaatt ctgctgatta acccgaccga ctccgacgca gtgggtaatg ctgtgaagat
     2461 ggctaactag gcgaacattc cggttatcac tcttgaccgc caggcaacga aaggtgaagt
     2521 ggtgagccac attgcatctg ataacgtact gggcggcaaa atcgccggtg attacatcgc
     2581 gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa ggcattgccg gtacatccgc
     2641 agcccgtgaa cgtggcgaag gcttccagct ggccgttgct gctcacaagt ttaatgttct
     2701 tgccagccag ccagcagatt ttgatcgcac caaaggtttg aacgtaatgc agaacctgtt
     2761 gaccgctcat ccggatgttc aggctgtatt cgcgcagaat gatgaaatgg cgctgggcgc
     2821 gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg gtcgtcggat ttgacggtac
     2881 accggatggc gaaaaagcgg tgaatgatgg caaactagca gcgactatcg ctcagctacc
     2941 cgatcagatt ggcgcgaaag gcgttgaaac cgcagataaa gtgctgaaag gcgagaaagt
     3001 tcaggctaag tatctggttg atctgaaact ggttgttaag cagtagtttt aatcaggttg
     3061 tatgacctga tggtgacata aatacgtcat cgacagatga acgtgtaata taaagaaaag
     3121 cagggcacgc gccaccctaa cacggtggcg cattttatgg acatcccgaa tatgcaaaac
     3181 gcaggcagcc tcgttgttct tggcagcatt aatgctgacc acattcttaa tcttcaatct
     3241 tttcctactc caggcgaaac cgtaaccggt aaccactatc aggttgcatt tggcggcaaa
     3301 ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga atatcgcgtt tattgcctgt
     3361 actggcgatg acagcattgg cgagagcgtt cgccagcagc tcgccactga taacatcgat
     3421 attactccgg tcagcgtgat caaaggcgaa tcaacaggtg tggcgctgat ttttgttaat
     3481 ggcgaaggtg agaatgtcat cggtattcat gccggcgcta atgctgctct ttccccatcg
     3541 ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag cgttattaat gcagctggaa
     3601 tcaccactcg aaagtgtgat ggcagcggcg aaaatcgccc atcaaaataa gactatcgtt
     3661 gcgcttaacc cggctccggc tcgcgaactt cctgacgaac tgctggcgct ggtggacatt
     3721 attacgccaa acgaaacgga agcagaaaag ctcaccggta ttcgtgttga aaatgatgaa
     3781 gatgcagcga aggcggcgca ggtactgcat gaaaagggta tccgtactgt actgattact
     3841 ttaggaagtc gtggtgtatg ggctagcgtg aatggtgaag gtcagcgcgt tcctggattc
     3901 cgggtgcagg ctgtcgatac cattgctgcc ggagatacct ttaacggcgc gttaatcacg
     3961 gcgttgctgg aagaaaaacc attgccagag gcgattcggt ttgcccatgc tgccgctgcg
     4021 attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt ggcgtgaaga gatcgacgca
     4081 tttttagaca ggcagaggtg acgcttggct acaatgaaag atgttgcccg tttggcgggc
     4141 gtttctacct caacagtttc tcacgttatc aataaagatc gcttcgtcag tgaagcgatt
     4201 accgccaaag ttgaagcggc gattaaagaa ctcaattacg cgccatcagc tctggcgcgt
     4261 agcctcaaac tcaatcaaac acataccatt ggcatgttga tcactgccag taccaatcct
     4321 ttctattcag aactggtgcg tggcgttgaa cgcagctgct tcgaacgcgg ttatagtctc
     4381 gtcctttgca ataccgaagg cgatgaacag cggatgaatc gcaatctgga aacgctgatg
     4441 caaaaacgcg ttgatggctt actgttactg tgtaccgaaa cgcatcagcc ttcgcgtgaa
     4501 atcatgcaac gttatccgac agtgcctacc gtgatgatgg actgggctcc gttcgatggc
     4561 gacagcgatc ttattcagga taactcgttg ctgggcggag acttagcaac gcaatatctg
     4621 atcgataaag gtcatacccg tatcgcctgt attaccggtc cgctggataa aactccggcg
     4681 cgcctgcggt tggaaggtta tcgggcggcg atgaaacgtg cgggtctcaa cattcctgat
     4741 ggctatgaag tcactggtga ttttgaattt aacggcgggt ttgacgccat gcgccaactg
     4801 ttatcacatc cgctgcgtcc tcaggccgtc tttaccggaa atgacgctat ggctgttggc
     4861 gtttaccagg cgttatatca ggcagagtta caggttccgc aggatatcgc ggtgattggc
     4921 tatgacgata tcgaactggc aagctttatg acgccaccat taaccactat ccaccaaccg
     4981 aaagatgaac tgggggagct ggcgattgat gtactcatcc atcggataac ccagccgacc
     5041 cttcagcaac aacgattaca acttactccg attctgatgg aacgcggttc ggcatagatt
     5101 tacgctgtct tttgatcaaa ttattaccat cggttgtttt cagaagcatg aacattgctg
     5161 ctgaagcaac agtaataatg cccatcgtga taaacgtata gtggaattgt tcgacagtcg
     5221 ttgtgccttc catcccttca taaatgcgaa ggacggccgc acttacagca acgcctaaac
     5281 taatcgacag ttgctgcgtg accgccagaa cactgttacc gctgctggcg ttgtcatcgg
     5341 tcagatcggc aagtgtgatg gtattcatcg cggtaaactg cgtcgacata gccatcccta
     5401 atataaacaa cggcaagatc agcatccata tcgccattgc cggtgattgc aaagagaact
     5461 gagcgatcat tagcccaata atcaccgtga tccccactaa tgtatggcga tagcccagac
     5521 gacgtaagac ttgggtaacc atcgattttg caataatgga acctaacgct gtcggtgcca
     5581 tcatacagcc ggcaataaac gcctgataac caaatcctac ctgtaacatc aatggcataa
     5641 ggaacggtac acagccggtc cccagacggg ttgcaatatt gcctacgata ccgatcgaga
     5701 aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt tggcgtacgt cgcgcatgga
     5761 gaatatagag aagcagtaac ccgatgctgg tgacaattac cgtcaaggca atccagctgg
     5821 cgacaatctt ttccccgaat agctctattc cgcttgaaaa gagaacaagg ctgaggccaa
     5881 acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt ggtgaaattg ggcatatgtt
     5941 tgcgcgcgta aagaaggccc gcaataccta tggggatatt aattaaaaat atccagtgcc
     6001 aggttgccca ggtgaccagc acgccgccaa gaacggggcc taaaattggc cccaccagac
     6061 ccggcatggc gacaaaattc aataccggaa gaagttcatt acgaggataa gcacgcagta
     6121 aggccggccg agcaacaggc atcatcattg cgtcgcctat cccttgaata acccggaaga
     6181 caaccagctg tggtagcgaa ttagaaagtg cgtaggccag agaacccaac gtgaacagac
     6241 tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc ggctagccat ccgcttaccg
     6301 gaataagcat cgccaccgtc agcgtataac tgatgatggc tgattgcatc gcgagaggag
     6361 aacgattaag gctatgagcg attgcgggta aggcggtatt cagaatagtg gcatcaagtg
     6421 cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc cgacatacta cgcttctttt
     6481 tatcgctcat tcaatgtcct gttatcgggt tatcacttat caggtgagcg tagcagcgcc
     6541 tgacaagctt taaatgccgc gtcgccatcg ctttggataa tcgcatcgac aatcgcctgg
     6601 tgcagatcca gctttatcac tgtgtcgctg gtaattgacg tgaagtaagt gtgataaacc
     6661 gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg cgctcatttc atagatatgc
     6721 tcatgccagg ccatatcgac ttcgatccag cgttcacggc gaaagttctc ttttaatgcc
     6781 gccatttcgg ccattaacgt attgagatgc gccttctgtt ccgcggtgcc aaccgttgct
     6841 gccagtaggc aggcttgcgg ctccagacag atacgcataa ccagaaagtg atcgatgacc
     6901 tgatgaaagt tctcttctgt tatccaccaa gtaagcaatt cctgatcaag aaaattccag
     6961 ttcgattgtg gcatgacccg agtaccaatt cgcggtcgcg gtaaaaccat cccttttgcc
     7021 gttaacgttt tgaccgcttc gcgtaccgct gtacgactca ctccaaattg ctcgcccagc
     7081 tcaatctcac cgggcaaaat ggtgccgggt tcatattcac cttttaagat ccgttgcgcc
     7141 agcttctcag ccagaacata cgaaaggttt ttctgtgcag ctaactgttg tgcgcttaaa
     7201 ggcattactt atcttccttt ttctttttat tcctccttag tatgccacca ggaagtgtga
     7261 ttacggttgc aaaaacggca aattgcttgt tttatggcac attaacgggg tattttgctg
     7321 aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc
     7381 tataatacgc caccactgac acggaacaac ggcaaatacg ccgccgggtc agcggggttc
     7441 tcctgagaat tccgacagag aaagcaaaaa taaatgattg actctgtagc gggaaagcgt
     7501 attatgcaca cccccgcgcc gctgagaaaa agcgaagcgc actgctcttt aacaatt
//
LOCUS       DAEEDS010000143         7184 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEEDS010000143 DAEEDS010000000
VERSION     DAEEDS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.145"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(296..1198)
                     /locus_tag="KLH32_003771"
     CDS             complement(296..1198)
                     /locus_tag="KLH32_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBE0140425.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KLH32_003772"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KLH32_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBE0140426.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="KLH32_003773"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="KLH32_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBE0140427.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="KLH32_003774"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="KLH32_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBE0140428.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="KLH32_003775"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="KLH32_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBE0140429.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="KLH32_003776"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="KLH32_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7184
                     /locus_tag="KLH32_003777"
     CDS             7090..>7184
                     /locus_tag="KLH32_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBE0140430.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1673 a         1811 c         1878 g         1822 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgct
//
LOCUS       DAEEDS010000144         6990 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEEDS010000144 DAEEDS010000000
VERSION     DAEEDS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.146"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..180)
                     /locus_tag="KLH32_003778"
     CDS             complement(<1..180)
                     /locus_tag="KLH32_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0140431.1"
                     /translation="MIVLILVFRLVIGVQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVAASQ"
     gene            291..2723
                     /locus_tag="KLH32_003779"
     CDS             291..2723
                     /locus_tag="KLH32_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBE0140432.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2790..4259
                     /locus_tag="KLH32_003780"
     CDS             2790..4259
                     /locus_tag="KLH32_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBE0140433.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4259..5920
                     /locus_tag="KLH32_003781"
     CDS             4259..5920
                     /locus_tag="KLH32_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBE0140434.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6141..6482
                     /gene="symE"
                     /locus_tag="KLH32_003782"
     CDS             6141..6482
                     /gene="symE"
                     /locus_tag="KLH32_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBE0140435.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6529..6849)
                     /locus_tag="KLH32_003783"
                     /pseudo
     CDS             complement(6529..6849)
                     /locus_tag="KLH32_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2056 a         1669 c         1609 g         1656 t
ORIGIN      
        1 ctggctggct gctacaccat gttgccgggc aacgagggag accgtcatcc ccggttcaaa
       61 gctctgctga acaattgcga tcttttcctg tgtggtacgc cgtctgcgtt tctccggccc
      121 taagacatca atcatctgta ctccaatgac tagtctaaaa actagtatta agactatcac
      181 ttaaataagt gatactggtt gtctggagat tcagggggcc agtctaatca tagccaggct
      241 attgatttta tgtacaatca gaatcgtcct gataaaagcg agaacccccc atggcggaac
      301 tgaacctaag taacctgacg gaagcagaca tcattaccaa atgcgttatg ccagccattc
      361 tcaatgcagg ctgggacaac acaacgcaaa tcagacagga ggtcaaactc cgggacggta
      421 aagtcatcgt acgtggtaaa gttgcggcac gcagaacggt aaaatctgct gacatcgtgc
      481 tgtaccacaa gccaggcatt cccttagccg tgattgaagc aaaagccaac aaacatgaaa
      541 ttggcaaagg gatgcaacag ggcattgaat atgcgcgcct gctggacgtt ccttttgttt
      601 ttgccaccaa cggtgatggc tttatcttcc gcgatgccac cgcagccgaa ggtgaatgcc
      661 ttgaaaagca aataacgctg gatgacttcc cctcccccgc tgaactctgg caaaaattct
      721 gcctctggaa aggatacaca caagctcagc ttccggtgat tactcaggac tattacgacg
      781 atggcagcgg caaatcgcct cgttattatc aacttcaggc aatcaacaaa accattgaag
      841 ccgtctccaa cgggcaaaac cgcgttctgc tggtcatggc gaccggaaca gggaaaacct
      901 ataccgcatt ccagatcatc tggcgcctgt ggaaatcaaa aaataaaaaa cgcattttgt
      961 tccttgccga tcgcaacatt ctggtcgacc aaaccaaaaa taatgatttc cagccatttg
     1021 gtacggcaat gaccaaagtc agcggacgca ccattgatcc cgcttatgaa attcacctcg
     1081 cgctctatca ggctataact ggcccggagg aagaccaaaa agcgtttaaa caagtcgcac
     1141 cagatttctt cgatctgatc gtgatcgacg aatgccatcg cggcagcgca tccgaagaca
     1201 gcgcctggcg agagatcctt gattatttca gttccgccac ccaaattggc ttaaccgcca
     1261 cgccaaaaga gacgcatgaa gtctccagca cggattactt cggcgatccg gtttacgtct
     1321 actcactaaa agaagggatc gaagacggct tcctcgctcc ttataaagtt gtccgtgttg
     1381 atattgatgt cgacctgcaa ggctggcgcc caaccaaagg gcaaactgac ttaaacggcg
     1441 aagtgattga cgatcgtatc tataaccaga aagatttcga tcgcacgatg gtgatcgacg
     1501 aacgcacaga actggttgcc agaaccatta ccgactacct caagcgcacc aatccgatgg
     1561 ataaaaccat cgtcttctgt aacgacatcg atcatgcaga acgtatgcgc cgcgccctgg
     1621 ttaatctcaa cccgcagcag gtgaaaaaga acgacaaata cgtcatgaaa atcaccggcg
     1681 atgatgaaat tggcaaagct cagttggata acttcatcaa cccgaaaaaa ccgtacccgg
     1741 ttatcgcgac cacttcagag ctgatgacca ctggtgtgga tgctaaaacc tgcaaactgg
     1801 tagtactaga ccagaacatc cagtcgatga ccaaattcaa gcaaattatc ggtcgtggta
     1861 cacgcatcga cgaacgttac ggcaaactct ggtttaccat cctcgacttt aaaaaagcca
     1921 ccgaactgtt tgccgatgag cgtttcgatg gcattcccga aaaagtcatg gataccacac
     1981 cagaggatat cgccgatcca gaatctgatt ttgaagagaa actcgaagaa atcagcgaac
     2041 atgacgacga acaggtaaca ggcgttgatg aaccgcctgc gccaccatac caggttaccg
     2101 ataccgatga tgtcggccca tttccggaag aagacgagaa gaaaatccgc aagtttcacg
     2161 tcaacggtgt agcggtgggc gttattgcac agcgcgttca gtattacgac gccgacggta
     2221 aactggttac cgaatccttt aaagattaca cccgcaaaac actgctcaaa gaatatgcct
     2281 cgctggatga ctttacccgc aagtggcagg acgccgatcg caaagaagcg atcattcacg
     2341 agctggagca acaggggatc atctgggaag tactggcaga agaagtcggt aaagatctcg
     2401 acccgttcga catgctttgc cacgtagtgt atggtcagcc gccgttaacc cgcaaagagc
     2461 gcgccgagaa cgtgcacaag cggaactact tcacaaaata ctctgaagca gcgcaagccg
     2521 tgctcgataa tctgctggat aaatacgccg atgcgggcgt acaggagatc gaaagtattc
     2581 aggtgctgaa acttaaacca ttcgacagca tgggcacctt accggagatt attaaaaccg
     2641 gatttggcga ccgtaacggg tataatcagg cgctcagcga gctggaaaac gaaatctacc
     2701 aattaccgcc ccgctctgct taacaactct cttttgcgcc ttctgcaacc atcatgttgc
     2761 agaaggcctt gtttttcatg gaatacaaca tgtctatcag ctcagtaatc aaatcattac
     2821 aagatattat gcgcaaagat gccggtgtgg acggcgatgc gcagcgtctc ggtcagctct
     2881 cctggctgct gtttttgaaa atcttcgatg cccaggaaga ggcgctggaa ctggagcagg
     2941 ataactatca atatccgatc ccacagcgtt atttgtggcg cagttgggcc gccaacgcgc
     3001 agggcattac cggtgattcc ctgctggaat tcgttaatga tgatctgttt ccggcgttga
     3061 aaaacctcac tgcgcctatc gataaaaacc cacgcggcta cgtggtgaag caggcgttca
     3121 gcgatgccta taactatatg aaaaacggta cgctactgcg ccaggtgatc aacaagctga
     3181 acgaaattga ctttaccagc gccagcgaac gccatctgtt tggtgatatt tacgaacaga
     3241 tccttaaaga tctgcaatct gcgggcaatg cgggcgaatt ctatactcca cgcgccgtca
     3301 ctcgctttat ggtggatcgc gttgatccga aactcggcga atccattatg gacccggcct
     3361 gcggtacggg cggttttctt gcctgcgcgt tcgatcatgt aaagaacaaa tacgtgaaga
     3421 gcgtcgccga tcatcagaca ctgcaacaac agatccacgg tgttgagaaa aaacagcttc
     3481 cgcacctgct ggcgaccacc aatatgctgc tgcacggcat tgaagtaccg gtacaaattc
     3541 gtcacgacaa caccctgaat aaaccgcttt cctcctggga tgagcaactg gatgtcattg
     3601 ttaccaaccc gccgtttggt ggcacggaag aagacggtat tgagaagaac tttccggcag
     3661 agatgcaaac ccgcgaaacg gcggatctgt ttctgcaact gattgtggaa gtactggcga
     3721 aaaacggtcg tgcggcggtg gtattgccgg atggcacact atttggcgaa ggtgttaaaa
     3781 ccaaaatcaa aaagctactt accgaagagt gcaacctgca taccatcgtg cgtttaccga
     3841 atggtgtgtt taatccctat accggcatta aaaccaacct gttgttcttt accaaaggtc
     3901 agccaaccaa agagatttgg ttctatgaac atccgtatcc ggcgggcgtg aaaaactaca
     3961 gcaaaaccaa gccgatgaag tttgaagagt ttcaggcgga gatcgactgg tggggtaacg
     4021 aggccgatgg ttttgccagc cgcgtcgaga atgagcaagc gtggaaagtc agcattgatg
     4081 acgtgattgc gcgcaacttc aatctggata ttaaaaaccc acatcaggcg gaaaccgtca
     4141 gccatgatcc ggacgaactg ttagcgcagt atgcaaaaca gcaggcggag atccagacgc
     4201 tgcgtaatca actgcgcgat attcttggcg ctgcgctgtc tggcaaggag gttaactaat
     4261 gagtgtggaa aagctgatcg ttgatcatat ggaaacctgg acctcggcgt tgcaaacccg
     4321 ttccaccgcc gggcgcggca gctccggtaa gattgatttg tatggtatta agaaattgcg
     4381 tgagctgatt ctggaactgg cggtgcgcgg taaactggtg ccgcaggatc cgaatgatga
     4441 accggcgtcg gacctgctga agcgtattgc ggcggaaaaa gcagagctgg tgaagcaagg
     4501 caaaattaaa aagcaaaaac cactgccaga aattagtgag gaagagaagc cgtttgaatt
     4561 gccggaggga tgggagtggg tgcacctccc tgatatttat tgctcaattt ctgagtcatc
     4621 tagaaaaata aaatcctccg aaatactacc tgagggaaaa tatcctgtta tcgagcaaag
     4681 ccaagaattt atttctggtt attgcaacaa tgaatgtctg ttaataaagt taaataatcc
     4741 agttatagtt tttggtgatc acacaaggaa tattaaattt attgattttg attttgttgt
     4801 cggcgctgac ggtgtgaaga ttttatcacc tatattaatt tgtgagcgtt tctttttttg
     4861 gcagttacga tcatttaaat tagatgtaag aggatacgcg agacatttta aagtattaaa
     4921 tagctgctta tttgcattac cccccattgc agaacaagag cgaatagttg aaaaagttag
     4981 ctctttgatg tccctctgcg accaactaga acagcaatcc ctgaccagtc tggacgcaca
     5041 tcagcaactg gttgaaaccc tgttgggaac gcttacagac agccaaaaca ccgcggaact
     5101 ggctgaaaac tgggcgcgta ttagcgagca tttcgacacc ctatttacca ccgaagccag
     5161 cgtggatgcg ttaaaacaga ccattctgca actggccgta atgggcaaac ttgtgccgca
     5221 ggatccgaat gacgaacccg cctctgaact gctcaaacgt attgcgcagg aaaaagctca
     5281 attggtgaaa gaagggaaaa taaaaaaaca aaaaccactg ccgccaatta gcgatgagga
     5341 aaaaccgttt gaacttccgg aagggtggga atggtgtcgt attggtaata tagttaatat
     5401 taaatctgaa ttagtatcac caaaagatta tttaaattta tatcaagtag cacctgatat
     5461 tattgaaaag ggcactggga gagtgatatc aaagagaaca gtgaaagaat caggagtcaa
     5521 agggccaaat agtcgctttt ataaaggaca aattgtttat tctaaaatcc gcccgtcatt
     5581 atccaaagtt tttttagcag agtataatgg tctttgcagt gctgatatgt atccattaga
     5641 ctgttacatt aatccaaatt acttattgaa atatatcctt tccattccat ttcttatgca
     5701 agtcaaaaaa gcagagaaca gaataaaaat gccaaaactt aattctgaca gtttttacaa
     5761 tattattgtt gcgattcccc cctataatga acaacaagca atatttgata agataaattc
     5821 tattgaagca gtatgcaatg gtcttatttc ttatattggg atttatcata aaacccaact
     5881 gcatcttgca gacgcactca ctgacgcagc gataaactaa ttccctcgcc ctcacaaaac
     5941 caataaagcg gctaaatagc cgctttattc acatcaccaa aatttatatt tcacgctaat
     6001 tttcatctta ttttcattaa tttttagctg gactttccct gtatttactg atgatgtatc
     6061 caggtattta gcacggtgcg gatgtgcgcc aacacactcg caccgctaat cacaatcact
     6121 atttatagag aatagtaatc atgactgaca cgcattctat tgcacaaccg ttagaagcag
     6181 aagtctcccc ggcaaataat cgtcaattaa ccgtgagtta tgcgagtcgt tacccggatt
     6241 acagccgtat tcccgccatc accctgaaag gtcagtggct ggaagccgcc ggttttgcca
     6301 ccggcacggc ggtagatgtc aaagtgatgg aaggctgtat tgtcctcacc gcccaacccg
     6361 ccgccgccga agagagcgaa ctgatgcagt cactacgcaa ggtgtgtaag ctgtcggcgc
     6421 gtaaacaaag gcaggtacag gaatttattg gggtaattac aggtaaacag aaagtcgcct
     6481 gatacattga cccggtagcg ccagtgctac cgggtattat tcgccgcgtt ataccaacgc
     6541 gctctccatc tgtttttcaa atgcctgacg aattaatccc ataatgtaag gaagttgtgc
     6601 gaggtcgctg aaaccaattt ctacatcgcc attaccccag cgcccaacat ttgtcacatc
     6661 tttggcaata cctttcggat cgaccagttc atgaaactgc atattaagcg acaatcgcag
     6721 tcggcttttt tgcggcacaa catcaacaaa attcgtttcc gctttaaacg caatatatag
     6781 cttcaatact tcctgcgtga tccctgcgtc caggcgcata acttcatcgc gaagatgatc
     6841 gaataacact gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca
     6901 gaaggagcta ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc
     6961 tgtcagaacg gttcttgaaa atcaactttc
//
LOCUS       DAEEDS010000145         6829 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEEDS010000145 DAEEDS010000000
VERSION     DAEEDS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.147"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..327
                     /locus_tag="KLH32_003784"
     CDS             <1..327
                     /locus_tag="KLH32_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HBE0140436.1"
                     /translation="NLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVRPEAITLSD
                     RGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYLEIHPYGMF
                     VLADAA"
     gene            complement(436..1368)
                     /gene="mmuM"
                     /locus_tag="KLH32_003785"
     CDS             complement(436..1368)
                     /gene="mmuM"
                     /locus_tag="KLH32_003785"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HBE0140437.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAKLLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(1355..2758)
                     /gene="mmuP"
                     /locus_tag="KLH32_003786"
     CDS             complement(1355..2758)
                     /gene="mmuP"
                     /locus_tag="KLH32_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HBE0140438.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            2966..3982
                     /locus_tag="KLH32_003787"
     CDS             2966..3982
                     /locus_tag="KLH32_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HBE0140439.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
     gene            4210..4527
                     /locus_tag="KLH32_003788"
     CDS             4210..4527
                     /locus_tag="KLH32_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HBE0140440.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHRVRVFPV
                     SVF"
     gene            complement(4477..4890)
                     /locus_tag="KLH32_003789"
                     /pseudo
     CDS             complement(4477..4890)
                     /locus_tag="KLH32_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(4814..5173)
                     /locus_tag="KLH32_003790"
     CDS             complement(4814..5173)
                     /locus_tag="KLH32_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
                     /protein_id="HBE0140441.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQVRLGHVVLA
                     GQQAYHESTGNGMGNPW"
     gene            complement(5201..5380)
                     /locus_tag="KLH32_003791"
     CDS             complement(5201..5380)
                     /locus_tag="KLH32_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HBE0140442.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(5385..5765)
                     /locus_tag="KLH32_003792"
     CDS             complement(5385..5765)
                     /locus_tag="KLH32_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBE0140443.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(5765..5986)
                     /locus_tag="KLH32_003793"
     CDS             complement(5765..5986)
                     /locus_tag="KLH32_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBE0140444.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            6169..>6829
                     /locus_tag="KLH32_003794"
     CDS             6169..>6829
                     /locus_tag="KLH32_003794"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBE0140445.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     NK"
BASE COUNT         1758 a         1916 c         1675 g         1480 t
ORIGIN      
        1 aacctgttcc cggcaacctt cagcgacgga tacgttgata tctacggcta tcatctgccg
       61 cgcccgctgc actttggtac acagggtgaa gggatggtcg gtgtgcgccc ggaagcgatc
      121 acgctcagcg atcgcggcga agagagccag cgctgcgtga tccgccatgt cgcctatatg
      181 gggccgcagt atgaagtgac ggtggaatgg cacgggcagg agatattatt gcaggtcaac
      241 gctacgcgtc tgcaaccgga cgtcggcgag cagtattatc ttgaaatcca tccgtacggc
      301 atgtttgttc tggcggatgc ggcataacgt tttgtggttt tgtaggccgg ataaggcgct
      361 tgtgccgcca tccggcaatg cctgatgcgc tgcgcttatc aggcctacaa tgaacgatgt
      421 agacccgata aaccctcagc ttcgcgcttt taacgcggcg atatccgcag gcgtggtgcg
      481 acagcacccg ccaatcaacc gtgcgccagc ggcctgccac tgcggcagat aatccgccag
      541 ctgcgcgcaa tgttcaccgt gatgatgcca ggttttgctc acggcatcgt aatgctcgcc
      601 cgagttcgga tacaccacca gcggcagcac cgttaaaccg tgtaaatgct gcaacgcagc
      661 ggtggtgttt tccagcgcaa tacagttaat gcctagcgcc accacctgcg gataacccgc
      721 cagcaacgca accacgtcac gcagcggcgt accgtcgctc aggtgttcgc tgtcgcgcag
      781 ggtaaatgag aaccacgccc gcgcacgcgg atatgcggtc aacagcttgg ccaacgcctc
      841 aatctcggaa aaattcggca gggtttcgca ggccagcaga tcggccccgg catccagcaa
      901 ggcttccacg cgcgggcgat gaaacgcctg aaatgcctca acgctacaat gataatcgcc
      961 acggtattca gagccatccg ccagatacgc gccgtaaggc ccgacggatc ccgccaccag
     1021 aagcgttccc gcctgcgggt tctctgccag atacgcttca cgggctttac gcgccagctc
     1081 cacgcttttg ccaatcagcg ctttcgactg cgcttcatcc agacctcgcg cggcgaagcc
     1141 cgccggcgtc gcctgatagc tggcagtgat cgcgcattgc gcccccgccc ggtagtaatc
     1201 aagatgcact tcgcggataa gctccgggtt ttctaccagc actttggctg accacaggct
     1261 gtcggctaag ttacaccctc gcgcttccag ctccgtcgcc atcgcgccat ccagcagcag
     1321 gatatcctgt ttatcaagaa gagcgcgtaa cggattattc tgcgacatgt tctggctcct
     1381 gttttgcgtt tcggggttga gtaaggaaat aagcaccata gcacaacgca acaaacggta
     1441 acccgcacca caacgcaatt ctctgcgctg gatcgaatgc cagcccaaca caggccacca
     1501 ggcacagcac aaaacctaat actggcacca gcggatacca cggcgcgcga taatgtaatt
     1561 cactcaatgc cttaccttgt tgcagatgac ggcgacgaaa aacaaaatgc gaggcgcaga
     1621 tactcagcca caccgctacc accgcaaacc cggagattgc cgacagcgca acaaataccg
     1681 tgtccggggc caccacgctg gaaaacagcg ccagcacacc accgagcata ctgaccgaca
     1741 gcgccgtcag tggcacgccg tttttcgtta ctcgcgcaaa acaggccggt agcgtacgtt
     1801 cattcgacaa cgaccacagc atgcgcccgg aggcatataa cccggagttc gctgcagaaa
     1861 gaatagccgt caggatcacg aagttaaaaa tatcagcggc gtacgggatc cctactttct
     1921 caaataccag cacaaacggg cttttctcca cgcccgcctg ctgcatcggg atcagcgctg
     1981 ccagcacaaa cacggtgccg ataaagaaaa taatcagtcg cgcgatggta gtacgaatcg
     2041 ctaccgggat aactttgcgc gggttttccg tttcaccggc ggcaatgccg ataagctcgg
     2101 tacccgaaaa agcaaagttc actgccacca tagtcatcaa aatcggtaag ccaccgtgcg
     2161 ggaaccagcc ttctgccgtg atattactca gccccggcgc gggcgagcca tcctgcatcg
     2221 gaataaagcc gaaaatcgcc gccccaccga ggatgataaa ggcgatgata gtgaccactt
     2281 tgaccagcga gaaccagaac tccccttcgg caaaaaagcg cgtggagata acattcagac
     2341 caaaaataat cgcgcagaac accacgcacc agacccatac cggcacctgt ggaaaccagt
     2401 actgcataca gaatccagcg gcggtaaagc tcgaacccag cgccacggtc caggtcagcc
     2461 agtaaagcca ggccacggta tacccggtag ccggaccaag atagcgcgcg gcataaacgt
     2521 gaaacgctcc ggtctccggc atcgcgaccg acagctcgcc cagacactgc ataaccagcc
     2581 agaccaccag cgcaccaatc agataggcca gcagcgttcc cgccgctcca gtggtggaaa
     2641 tgatgtaccc ggtattgaag aataatcctg tgccaatcac gccgcccaag gaaagcataa
     2701 tcaggtgacg cgttttcatt gtgcgcttca gtggcgcatt ttgttgtgtt gtttgcatat
     2761 aaccttctaa acgtatggac atctaaacat ccaaagaagg tttgttatac cgcgtttgtt
     2821 aacagaatgc aaagtcaacc ctgcaccaga cgcagatgcc cgcgccgggc gcttaatagc
     2881 gggtggggat taataatcag atacaaaaag ggccgccagc tcataacgcc tccggaaggt
     2941 gcgaacaagt ccctgatatg agatcatgtt tgtcatctgg agccatagaa cagggttcat
     3001 catgagtcat caacttacct tcgccgacag tgaattcagc agtaagcgcc gtcagaccag
     3061 aaaagagatt ttcttgtccc gcatggagca gattctgcca tggcaaaaca tggtggaagt
     3121 catcgagccg ttttacccca aggctggtaa tggccggcga ccttatccgc tggaaaccat
     3181 gctacgcatt cactgcatgc agcattggta caacctgagc gatggcgcga tggaagatgc
     3241 tctgtacgaa atcgcctcca tgcgtctgtt tgcccggtta tccctggata gcgccttgcc
     3301 ggaccgcacc accatcatga atttccgcca cctgctggag cagcatcaac tggcccgcca
     3361 attgttcaag accatcagtc gctggctggc cgaagcaggc gtcatgatga ctcaaggcac
     3421 cttggtcgat gccaccatca ttgaggcacc cagctcgacc aagaacaaag agcagcaacg
     3481 cgatccggag atgcatcaga ccaagaaagg caatcagtgg cactttggca tgaaggccca
     3541 cattggtgtc gatgccaaga gtggcctgac ccacagccta gtcaccaccg cggccaacga
     3601 gcatgacctc aatcagctgg gtaatctgct gcatggagag gagcaatttg tctcagccga
     3661 tgccggctac caaggggcgc cacagcgcga ggagctggcc gaggtggatg tggactggct
     3721 gatcgccgag cgccccggca aggtaagaac cttgaaacag catccacgca agaacaaaac
     3781 ggccatcaac atcgaataca tgaaagccag catccgggcc aaggtggagc acccatttcg
     3841 catcatcaag cgacagttcg gcttcgtgaa agccagatac aaggggttgc tgaaaaacga
     3901 taaccaactg gcgatgttat tcacgctggc caacctgttt cgggcggacc aaatgatacg
     3961 tcagtgggag agatctcact aaaaactggg gataacgcct taaatggcga agaaacggtc
     4021 taaataggct gattcaaggc atttacggga gaaaaaatcg gctcaaacat gaagaaatga
     4081 aatgactgag tcagccgaga agaatttccc cgcttattcg caccttccct agaacaacaa
     4141 tgcctcgatt ggtttaaaga gctaggttac caataccaaa atggctatga catcgcacca
     4201 gatggtgata tgcctgaacg tgaaaactat cagcaagtca cgctacaggc gcgtttagtc
     4261 tctgccttgg aaaccttaaa cccaaatgtg cccagtgaca ccttaatcga agtagcgaaa
     4321 atcgttgcca caccgcaaac gcctgtactg attaaaaaca acaaactctt tcaccaatat
     4381 ttaatcgaag gtgtgcctgt tgaatactcg gtaatggagc aagaacaatg ggtgaccaag
     4441 catactcatg ttcgattaat tgattttgtg ttgtggtcaa ctaaaactgg ccaccgcgtt
     4501 agagtttttc cagtatcggt tttctgattc gtttggtggt aacccaccat tatattcgtg
     4561 cggtcttagt gcgctgtaat atccaacgat atagtccgtt atggcgtgag ctgcatcgct
     4621 gaaacttaca tagcccgtcg ctggcaccca ttcgttcttc agactcctga agaagcgctc
     4681 cattgggctg ttatcccagc agtttccacg ccgactcata ctctgcctga tccggtatcg
     4741 ccacagtaac tgccggaact gcctgctcgt ataatgactg ccttgatcgc tgtggaacat
     4801 caccccgacg ggcttaccac gggtttccca tgccatttcc agtgctttca tggtaagcct
     4861 gctgtccggc gagaacgaca tggcccagcc tgacctgccc ctctttcctg cgcaaccacg
     4921 ctaccagcga gtcaattcca ccaatacggc gaaacagttc atcgctgtta aaaaacggct
     4981 gcaacgcctc atttgttgcc atagtttgct cggaaacaac gaggccgtcg tccatgtgct
     5041 cgattaactc acgcggcacc ggctccataa taaatttacg gccagcctcc accagctttc
     5101 tgacttcctg atccactttg aatgtggcga cgccaagctc tttttgtaca aagggagtaa
     5161 ttacggcttt cacatcacac ctttcatcac tgattgggct ttatctgctg cccggcattc
     5221 tctgtttaag cacaaccatt tcctgacggc ataacacagc aatagcggtc ctggcaccaa
     5281 tttgcttacc agccaggtat tgctttacct cgcggcgact cacgccatca agaagcatct
     5341 ttaacgcttc acgggacaat ttgttgtatt tgcgtgccat taatctactc cgcagaacca
     5401 tacaatctac gtaacgtgtc ggcgacagaa gatacagata tctctccggt cgcagcccct
     5461 acggtaaggt ctgccagttc aggtgaatca aatacctgca ccccgttacg gcgtagaaat
     5521 aacagcgcac tgtttagcgc ggtacgctta ttggcatcat tgaatatatg ccctctcgct
     5581 gtagccacca ggtaggtggc ggagacttcg aaaaggtcgg tgatctcttc gtaggcaact
     5641 ctggcctgaa ctctcccgat aatggcctct gccctgcccg gatcagacat tcccggcagg
     5701 ccgccgtagc ggtttatatt cgcatcatga agcgcaataa gttcttccgg tgatatatgc
     5761 ctcattatcg gttaaccagt tccttgttgg tggagtccag ggtgtcaaac agggatgcaa
     5821 attcagcatc cagcgccgct tttttgtagg cttcgaaagt agccttgctg acaattactg
     5881 ctggctcacg gcctctgcgg gtgatttcaa cctcttcccc ggcctcaaca ttgttgagca
     5941 cttcagaaag gttgccgcgc gcggtacgga agttaatgga ttgcataaac acctcgtgta
     6001 ctcgttatgt gtacacaatt ataaacttca caggcataaa gcaccagcac tttgcggctt
     6061 aacaaacctc taggcaggtc attcgtagcc taatgtccga actgctaaag catccaagtt
     6121 gctgtagaat caccgccaat tacataagcc tgaaataagt ggatgaaaat gacaagtatt
     6181 caacaacgtg cagagcttca tcgtcaaatc tggcaaattg ctaacgatgt caggggttcg
     6241 gtcgatggat gggattttaa gcaatacgtt ctgggcgcac ttttctaccg ttttatcagc
     6301 gaaaattttt ccagttatat tgaagccggt gatgacagta tctgttatgc gaaactggat
     6361 gacagcgtaa ttactgatga cattaaagac gatgccatca aaaccaaagg ctacttcatc
     6421 tacccaagcc agcttttctg caacgtagct gcgaaagcaa ataccaatga cagattgaat
     6481 gcagatttaa acagcatctt cgttgctatc gaaagttctg cttacggtta tccttcagaa
     6541 gctgacatca aaggtttgtt tgctgatttc gataccacca gtaaccgcct gggtaacacc
     6601 gttaaagata aaaatgcccg cctggctgcg gttctgaaag gggttgaagg gttaaaactt
     6661 ggtgacttca acgaacatca gattgacctg ttcggtgatg cctatgagtt cctgatttct
     6721 aactatgcgg caaatgccgg taagtcaggc ggtgagttct ttacaccgca gcacgtctcc
     6781 aagctgattg cacaactggc tatgcacggg cagaccagcg ttaacaaaa
//
LOCUS       DAEEDS010000146         6733 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEEDS010000146 DAEEDS010000000
VERSION     DAEEDS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.148"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..306)
                     /locus_tag="KLH32_003795"
     CDS             complement(<1..306)
                     /locus_tag="KLH32_003795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140446.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     "
     gene            complement(392..1273)
                     /gene="narH"
                     /locus_tag="KLH32_003796"
                     /pseudo
     CDS             complement(392..1273)
                     /gene="narH"
                     /locus_tag="KLH32_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1270..5010)
                     /gene="narZ"
                     /locus_tag="KLH32_003797"
                     /pseudo
     CDS             complement(1270..5010)
                     /gene="narZ"
                     /locus_tag="KLH32_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5092..6480)
                     /gene="narU"
                     /locus_tag="KLH32_003798"
     CDS             complement(5092..6480)
                     /gene="narU"
                     /locus_tag="KLH32_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBE0140447.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVTVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1601 a         1768 c         1776 g         1588 t
ORIGIN      
        1 accgccggcg ctgataccgg caagaatggt cgcaaattcc cgctccgtgc agcgggcgat
       61 ttccggatat ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc
      121 atccacaaag aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc
      181 aaagcgcaga acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac
      241 atagcgggga agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg
      301 ggccatataa ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac
      361 catgacaagc cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc
      421 tcgacgcttc ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac
      481 ccacgcaggt ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga
      541 tacatttttc tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc
      601 taatgcacaa acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt
      661 cgcgtttgta gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt
      721 cgcacaggcg cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt
      781 gcatggcctc gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt
      841 cccagtttgg gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc
      901 gagcagtagg gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga
      961 aaggttcgta gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta
     1021 ttacgcccat cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc
     1081 cgccttgcca ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt
     1141 cgacgttgtt aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg
     1201 tcaccgaaca ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg
     1261 aacgtatttt cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt
     1321 aacgttcttc atcttgcgga tcatgatgaa ttcgtcgcgg ttcgagccga cggtgccgta
     1381 gtagttaaag ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca
     1441 aacgcgggtg accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat
     1501 attcataatg cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct
     1561 gaccaccgcg cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc
     1621 gacaatggtc agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga
     1681 gagcgtcagc attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg
     1741 cgtcaggaag ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact
     1801 gacgctacgg gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat
     1861 ccacggatga tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc
     1921 atgaacgttg gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt
     1981 ggggctggag ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc
     2041 ctctttgtgc agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca
     2101 cgctttgact gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc
     2161 gtcaatggcg gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt
     2221 gagtttgccg aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt
     2281 gccaagtttg tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc
     2341 gcgctccacc gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt
     2401 gcgccagtcg agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg
     2461 ttgtaatacc acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt
     2521 ggcaatacct ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc
     2581 agaaagcgga tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca
     2641 ggtggcggtg ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag
     2701 cgtcaccagc aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg
     2761 tttgatcccg tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag
     2821 atacttctgc atatactcgt ggcctttgcc ggaggagcca agcaggttag agcgccagac
     2881 aaacaaatta cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc
     2941 gcccgatttc agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc
     3001 agctttaatc ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg
     3061 ttcggcgcga acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg
     3121 tgaaagtaac tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa
     3181 aaacgaggtg ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg
     3241 caaccagccg gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc
     3301 gctttgcccg acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc
     3361 catgtgatac cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt
     3421 atgggcggta tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt
     3481 aatttgctca ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt
     3541 ttcatcttcc agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac
     3601 caccggacag gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag
     3661 caccggattg tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa
     3721 ggccacgcct gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt
     3781 accggcggca atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga
     3841 accgttcggc actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt
     3901 gttgctttcg cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta
     3961 gctaccatcg tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca
     4021 gtagttgata aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc
     4081 cattgccatc gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa
     4141 tttggccact tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac
     4201 ttcggtaaag aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat
     4261 gatgtagctg gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt
     4321 catcggcgag gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc
     4381 aagcagcgac agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg
     4441 cgagaaaccg gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc
     4501 aatcagctgg tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg
     4561 tttgtagctc aggcactttt gcggatcgtt cataatcgat gcccacgcca gtaccggatc
     4621 gctgtgttgt ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta
     4681 tttcaggcgg ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg
     4741 aggttcatga ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca
     4801 ggtcaccaga ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc
     4861 gtgggtggaa cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc
     4921 gcggttgcta tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa
     4981 gtagcgaaag cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg
     5041 gatctgatgc cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg
     5101 gctgaacttc cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat
     5161 taaaaacact ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc
     5221 ctgcggaata aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc
     5281 cgtttcggtg acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg
     5341 ataaatggtt atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag
     5401 acccgcggtc agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc
     5461 tgtgcccggt aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat
     5521 caacgtcacc cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat
     5581 ggcaccaata aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt
     5641 ttttgccagc atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta
     5701 aagcaggctc agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga
     5761 ggcgcgtgaa ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag
     5821 tagcggtacc caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg
     5881 tacgccattg acgccgagaa aggcaaatac agttacaaaa atgaccagcg gtgcaaccag
     5941 ctgcattaca cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg
     6001 tttggctttt ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc
     6061 aaaaccgcat agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac
     6121 ggcaattccg agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg
     6181 gcgtccaccg aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac
     6241 ggagggtaat gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt
     6301 gagattaacg gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact
     6361 gactgatatc cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg
     6421 attttctggt ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat
     6481 attgttcctc atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa
     6541 tagccagtta ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag
     6601 aaagcgtaac cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta
     6661 actaacaagc taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct
     6721 tcagatcgtg aat
//
LOCUS       DAEEDS010000147         6615 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEEDS010000147 DAEEDS010000000
VERSION     DAEEDS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.149"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(97..2100)
                     /locus_tag="KLH32_003799"
     CDS             complement(97..2100)
                     /locus_tag="KLH32_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140448.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2097..3233)
                     /locus_tag="KLH32_003800"
     CDS             complement(2097..3233)
                     /locus_tag="KLH32_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE0140449.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3226..5505)
                     /locus_tag="KLH32_003801"
     CDS             complement(3226..5505)
                     /locus_tag="KLH32_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140450.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5516..6604)
                     /locus_tag="KLH32_003802"
     CDS             complement(5516..6604)
                     /locus_tag="KLH32_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBE0140451.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1512 a         1823 c         1777 g         1503 t
ORIGIN      
        1 actggtgact ctcaccaggg gctatatagg atatgcctaa taccgtggcg tgagcagtcc
       61 ggaactggag tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg
      121 gaataattgt tcgcaaagca aaacaccgtt attcattgct tctacccgtg cctcgctttc
      181 tgtattacga aattgtgcca acacatgtgc cagccgataa aaacccaccg cggtgaggtc
      241 attcgccagc aactctgcct gattaatagc gctctgttcc tgatagcgcc agccgttatg
      301 gagcagttga ataagtaacg cctggcagcg catcagcaac tgatgagcag tagacggcac
      361 aggcagaacg ctggcagaag atagtgatac caccacaggc gcagtttctg cgtccagcgc
      421 ccaggcacga gtctttgtca tcattacctg tggttccagt gtcaattgcc catcaacaaa
      481 actgacaaag ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa gcgccgccat
      541 gcgttcaatg gcataaggcg cgctggctga ggctggcagt gataacgtca gcagattatc
      601 ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag
      661 cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat
      721 gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa
      781 cggcgcgctc aacatttgcc aggcatcagg cgacagcggt acaacgctgc ttaagcggtt
      841 gcgggtagct aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg
      901 cccacccgcc agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtc
      961 ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc
     1021 ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga
     1081 caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg
     1141 ccacggcaca ggaggaactt caccaacact gtcgctccgg gccatctctt gtgcgcaatt
     1201 taatcgactg tttaatgccg caagctgacg taagcattct ccggcatgat agtggctggc
     1261 gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt agcgattcac tcacccatcg
     1321 ccagctgcag cgttccgctg cttgctgcgc gcggctgaaa gcggcctcgt agtgaataag
     1381 cggctggctg atgccgctca gccataatgc ctggcttagt tgttgaacat attgacgaca
     1441 cgttttgcct tcttcgctgg caaacggatc gtcagatgat gtgacgtgtt cgctgcgcat
     1501 ctgccagatt aaatgagtca attccgcttg ctgcgctttt gcctggacaa aggcctgtac
     1561 tgccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat
     1621 actgctgcgg gaataaaaac gcacatcgct catcggtaag cgggcagagg gaatttcacc
     1681 cggcgcacag aacaactcaa tggtgatgct tttagcgacc agcgcctgtg cgcgtttgcg
     1741 ggtggcatcg ggaagggtag ccagttcttc cagccagatt gccggatccc attcctcttc
     1801 ttttcccgta ggctgggcgg tagcacaaag tcgttgataa cttaacacca gcatcacgcg
     1861 atgacggcac atgccgctgg ccccataggt gcactgagcc tctttcagtg cctggccgtt
     1921 cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgctgt tctcatggct
     1981 gatttccggg acgttgccat tttccagttc cttaaggctg cgcttaacaa aaccggcatt
     2041 gcttaacgcc gtcagtgcct gcggtgtcag ttctaataat tccggacgta gtgaattcat
     2101 gactgaagat tctccgcaag ccatgatgcc agctcgcccg gtgtcatggc ggctatttgt
     2161 gcgccgacat taatcagcgc ctgggccgta tcgcggtcat agcaaggtgt tgctgtgcta
     2221 tcgagcgctg ccagtcccag cactttgatg ccgctctgga cacacttttt cacttggtgc
     2281 gtcagcaatg atgatgaacc cccttcataa aaatcgctca cgaggataat gacgcttttt
     2341 gcaggttgtt caataagttg ccgaccatac tccatggcac tggcgatatt ggttccgccg
     2401 cctaattgca ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg
     2461 acgcttgtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca taaacaggcc
     2521 gccatcaccg cagagtggat caccgaatcg accatcgatc cgctttgatc aaccagtaag
     2581 accagttgcc attgctcgct ttggcgttta atgcggctgt taaagcgggg gaattcgata
     2641 tacaacttgc cgtgttgcgg gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa
     2701 tcaaagtttc gcgccagtgg aataaatgag cggcgacgac gatcgcggac accagaaaaa
     2761 gcctgacgaa cttcctttgc cagtcgcgcc ataatttctt ccacaacctg gcgcacaatc
     2821 ctgcgggcgg cagccagtac ttcggggttc atcagatgtt tggtgtgcaa aacggcgcgt
     2881 agcaggcttt cggaaggctg catacgttcc agcacgtcga gatttgtcac cacatcttca
     2941 atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc
     3001 gtgtgaatac tgttgatcca ctcaggagtg gtgagatttg agccacctaa tccactagag
     3061 cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtgatct
     3121 atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt
     3181 aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac
     3241 tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc
     3301 ttgcggtgga caatgcaacg gcatttgcag tgccgaaacg ggaagttgcg ccagttgata
     3361 atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt ggcggtagcc aggccattgc
     3421 cgcgcgtaaa tcgggcaggg cattggtaaa atcggcatca cttagttgat ttaaatgact
     3481 gctgaaaccg gcgataaatg ccggctgaca ggccagttga tgacgggcta atgccagcaa
     3541 accgtgcagc gcctcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc
     3601 ttcggcacta gcgttgggat gttccagacg catcagcgca ccaagagtcg cgccgcgatc
     3661 cagtgccagg acatgaatcg cctgactgtg tcgctccagc aaggctaccg ccgcagaaag
     3721 cgaaacgccg gggagttgca caccgctatg tagatcgcgc aggatatgac acaacgcttg
     3781 ccagctatgc aagtgagcgt gaaactcctt ttcatccggt ctgccgttgg attcacacag
     3841 ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat
     3901 accgctaatt tcatccagcc gccataaggc atatagcact tccagcgcct ggcccatttc
     3961 ggcaaattga ttttcctggg cgattaataa tgtgagttgc tccagtaatt gttgactgaa
     4021 ggacgccaga cccgctgacg ccgcttggct aagacatgtg gtaatgctgc cgattccacc
     4081 cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa
     4141 gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc
     4201 ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc
     4261 cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt
     4321 taaggtaagc tccgccggaa gagagattgc ggtacgttcc agttcgcaag tgacatcttt
     4381 tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt
     4441 gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca
     4501 cggcaacggc gcattcaggg cttctttaat cagcgatcct gctatggcat ccagccagtc
     4561 agtgcgtaac ggtagtgtat gaccgcgcaa ctgtgccagt gccatcgcat gcagatgagc
     4621 ggcagccata tccgcggtcg aagcaggcaa cttgtgctgg cgcaaacggg tgagaaccgt
     4681 ttttagcagt tgttctcccg cctgctgtaa gccccactgc cagcaccagt tttgccagac
     4741 cggggcaggc attcctgaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg
     4801 ggtgagataa caacctgtaa cggcatctgc cagcgacggc aattctggcg tgttaattac
     4861 ctgcggacat tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacaac
     4921 taacacgtcg ccattatttt gctgcatcgc ccatccaatc cagcgggcca taaaggcttc
     4981 gcgctgacga tttagagcat cgccggggga gtcgccccgc agttgggcaa aatagtgcgc
     5041 cagtgcagag ggtaatgcag tttgctggct ttcatcttcg aacaagtgat cccacagggt
     5101 gtcgctgtta tccatgcggg tggcacgcag cagcaaggtc tggctttcct cttgcgtatc
     5161 aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgatga agtaagtttg
     5221 tgcctgaata cgacgatcgg cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg
     5281 cgtccaggca ccacgcccgg gggctgcacc gtcctgatac tggcaataac tgtaaatagc
     5341 taccggaagc tggtgggcta aaaacagttc gtctacccgg tcattaaaat cagcagggcc
     5401 ttcaatcaac acgtatcgtg gccgctggct ttcgattaac gatttcacca gccgggcgca
     5461 ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcatc tttcctcacg
     5521 gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac
     5581 gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc
     5641 cggcaatgca gtccaccagg tcggctggct cacctgcgcg attcgctaaa aaccaggcgc
     5701 ggacgcctac ggcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgttagtg
     5761 tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cggaccagta
     5821 actccagtac cgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg
     5881 aagcactggc gacaagctcc agctcctggg cgaaatccat aatcggaaag acagtttcaa
     5941 aatcgaagcg acgctttagc gcagcactca tttcattgac gccgcgatcg cgggtattgg
     6001 cagtggcaat aatattaaag ccttctcgcg catagagctg actggcttca ccagtgagtt
     6061 ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgc acttccagcg
     6121 gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc aggtaaagcg
     6181 gggcagggac cagcgcttcc gttgatggac catgattgat aagcagggcg tagttccagc
     6241 cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc
     6301 cgctaataga ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca
     6361 ccaacatcaa accgcgtccg gtggcaagag tgactaacat acgttcgaca gcggagggac
     6421 taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg
     6481 caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg
     6541 ccagttcatc ggcgtataac acagcagcag gcggacgctg cagatgatta ttctgcggtg
     6601 acatatattc atccc
//
LOCUS       DAEEDS010000148         6402 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEEDS010000148 DAEEDS010000000
VERSION     DAEEDS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.150"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KLH32_003803"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KLH32_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBE0140452.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="KLH32_003804"
     CDS             complement(1433..1759)
                     /locus_tag="KLH32_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBE0140453.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="KLH32_003805"
     CDS             1894..2235
                     /locus_tag="KLH32_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBE0140454.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="KLH32_003806"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="KLH32_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="KLH32_003807"
     CDS             complement(2833..3543)
                     /locus_tag="KLH32_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBE0140455.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="KLH32_003808"
     CDS             3651..3956
                     /locus_tag="KLH32_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBE0140456.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="KLH32_003809"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="KLH32_003809"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="KLH32_003810"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="KLH32_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1706 a         1628 c         1500 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tg
//
LOCUS       DAEEDS010000149         6375 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEEDS010000149 DAEEDS010000000
VERSION     DAEEDS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.151"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            59..1385
                     /gene="pgaC"
                     /locus_tag="KLH32_003811"
                     /pseudo
     CDS             59..1385
                     /gene="pgaC"
                     /locus_tag="KLH32_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1387..1800
                     /gene="pgaD"
                     /locus_tag="KLH32_003812"
                     /pseudo
     CDS             1387..1800
                     /gene="pgaD"
                     /locus_tag="KLH32_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="KLH32_003813"
     CDS             complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="KLH32_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBE0140457.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="KLH32_003814"
     CDS             complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="KLH32_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBE0140458.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="KLH32_003815"
     CDS             complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="KLH32_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBE0140459.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5702..>6375)
                     /locus_tag="KLH32_003816"
     CDS             complement(5702..>6375)
                     /locus_tag="KLH32_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBE0140460.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1533 a         1681 c         1534 g         1627 t
ORIGIN      
        1 aaccaacctg aaatagacct tattcgtcct gagttttcaa cagcctggta tccgaaaaat
       61 gattaatcgc atcgtatcgt tttttatatt atgtctggtg ttatgcatac ccctatgcgt
      121 agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat
      181 gtccattatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc
      241 gtggggagaa aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat
      301 tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg
      361 ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat
      421 cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaaaca
      481 ggggaaagcc attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg
      541 cattgatggc gatgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt
      601 gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac gttctaccct
      661 ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca
      721 gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc
      781 cctggcagaa gtgggttact ggaatgacga tatgatcacc gaagatattg atattagctg
      841 gaagctgcag ttgaatcagt ggccgatttt tttacgagcc acgggcgctg tgctggatat
      901 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
      961 cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc
     1021 cgctgttttt tgaatactgc ctgacgacaa tatgggcctt cacctgcctg gtcggtttca
     1081 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     1141 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     1201 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     1261 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     1321 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc tgatcgcggg attctgagag
     1381 gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt actggttgat
     1441 tatgttgcca caaccatctt gtagacatta tttgctttgt tcatattctt attcgccatg
     1501 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     1561 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     1621 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     1681 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac
     1741 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     1801 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     1861 catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg aaccggacga
     1921 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     1981 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     2041 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     2101 caactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcggtt tccaccttcc
     2161 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcaagt
     2221 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa
     2281 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     2341 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     2401 tggcgaatat cagctgctta ctctcaatag cctgcagata gtgcaattgc gcttcatttc
     2461 gcgcaaggat gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     2521 tcatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     2581 gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg
     2641 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac
     2701 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     2761 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagaaagga agtgagaaaa
     2821 atacagagtg acgaagttgc taccggagga gaaatctccg cgagtggtgg agcgttgctt
     2881 aacaaaacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     2941 actgccggga acttccgctg ctacttaata agtacaacag aactcgcatt cattgcaaca
     3001 atatattcac ttgtatttaa cgataaacac ccatttcggt gacattaatt tctactggaa
     3061 agatgacaga gtgatgacag tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg
     3121 ctggtaaaat gtgcctcgtg tttgccggat tcggcgcgaa cgccttaccc gacctacaag
     3181 tgattaataa tttgtgcaca aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc
     3241 gtcgttcaaa cctgcaatag cgcccttccc agataatcgt tcgcgtcctt cactcccggc
     3301 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     3361 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     3421 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3481 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3541 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3601 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3661 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3721 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3781 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     3841 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     3901 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggtt caccgtgtcc
     3961 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     4021 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     4081 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     4141 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     4201 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca aacgaaacaa ccgctcaaga
     4261 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     4321 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     4381 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     4441 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     4501 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     4561 ccagcgcagt aatcggccct ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg
     4621 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     4681 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     4741 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     4801 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     4861 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     4921 cggtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     4981 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
     5041 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatac
     5101 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     5161 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     5221 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     5281 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     5341 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
     5401 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
     5461 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
     5521 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
     5581 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
     5641 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
     5701 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
     5761 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggtgct tcctgatagc
     5821 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagaggacc gcgctcatat
     5881 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
     5941 cagccgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
     6001 caagattgag gcgaataccg ccccagtaga gcaggaagcc gagcaccacg gcagtagcaa
     6061 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
     6121 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
     6181 cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgttcc agttgcactt
     6241 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
     6301 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
     6361 cgttaatgaa gatcc
//
LOCUS       DAEEDS010000150         6355 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEEDS010000150 DAEEDS010000000
VERSION     DAEEDS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.152"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..370
                     /locus_tag="KLH32_003817"
     CDS             <1..370
                     /locus_tag="KLH32_003817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140461.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KLH32_003818"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KLH32_003818"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBE0140462.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KLH32_003819"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KLH32_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBE0140463.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KLH32_003820"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KLH32_003820"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBE0140464.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KLH32_003821"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KLH32_003821"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBE0140465.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KLH32_003822"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KLH32_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBE0140466.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..>6355)
                     /locus_tag="KLH32_003823"
     CDS             complement(5095..>6355)
                     /locus_tag="KLH32_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBE0140467.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1439 a         1837 c         1525 g         1554 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaggaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagtt
//
LOCUS       DAEEDS010000151         6332 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEEDS010000151 DAEEDS010000000
VERSION     DAEEDS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.153"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            5..436
                     /locus_tag="KLH32_003824"
     CDS             5..436
                     /locus_tag="KLH32_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBE0140468.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KLH32_003825"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KLH32_003825"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBE0140469.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KLH32_003826"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KLH32_003826"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBE0140470.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KLH32_003827"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KLH32_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBE0140471.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="KLH32_003828"
     CDS             complement(4438..5145)
                     /locus_tag="KLH32_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBE0140472.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KLH32_003829"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KLH32_003829"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBE0140473.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="KLH32_003830"
     CDS             complement(6003..6206)
                     /locus_tag="KLH32_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0140474.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1406 a         1810 c         1648 g         1468 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct ta
//
LOCUS       DAEEDS010000152         6269 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEEDS010000152 DAEEDS010000000
VERSION     DAEEDS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.154"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..667)
                     /locus_tag="KLH32_003831"
     CDS             complement(<1..667)
                     /locus_tag="KLH32_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HBE0140475.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(657..2414)
                     /locus_tag="KLH32_003832"
     CDS             complement(657..2414)
                     /locus_tag="KLH32_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBE0140476.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2430..3314)
                     /locus_tag="KLH32_003833"
     CDS             complement(2430..3314)
                     /locus_tag="KLH32_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE0140477.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3325..3930)
                     /locus_tag="KLH32_003834"
     CDS             complement(3325..3930)
                     /locus_tag="KLH32_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE0140478.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4101..>6269)
                     /locus_tag="KLH32_003835"
     CDS             complement(4101..>6269)
                     /locus_tag="KLH32_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBE0140479.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1836 a         1568 c         1316 g         1549 t
ORIGIN      
        1 atggcagtgc tattttgatt cgacgttgat caagtttctg ataattccaa cgacggtcga
       61 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
      121 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
      181 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
      241 tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc
      301 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa
      361 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgttgttcga
      421 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
      481 caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
      541 catggctgtt aaggtcctga accgcggtga actgattaag agatccatag gtgaagaaga
      601 aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc cgacgttcag
      661 ttatcacgac gcaccgccat cgaaaccgta aaaatgcccc acacatcaat ccgttgagtg
      721 catttatcaa atccggcatt acgcacgagc gaatccatct ccccttgcga acgcacgcgc
      781 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt
      841 gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc attggctaga
      901 cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatatag gccagagaca
      961 atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc
     1021 tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca
     1081 acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc
     1141 gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct
     1201 ttggcgtgga gatcggcaac ggcttgttta atcaatattt gcaaatgggt tttgcgctgg
     1261 cgaataccgc gccatccaac actgttcagg tagattttgt cgattaaacg cccgaatgcg
     1321 ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga atcaaagccg
     1381 gtactcattc cgagatgcag gcccgcagag tgcgcaccga acaatttcat cgcctttcgc
     1441 ataaagcgat acgctaaatc aaccggcgat aatggcacgg gtccaccaga aaggagccgc
     1501 catcgatccg ctgatggtcc tgtgcggtct tcatgctgat aatcaaattt ttgcgatttg
     1561 ttagcatata agcggcagat aaagctttgc attttttcaa atgcctgcgc cctgttctct
     1621 tcacccaatg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
     1681 aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc acctgatatc
     1741 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
     1801 tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa
     1861 ctcgccaccc gttgccgatc gtgggtcaaa tagcgccctt tcacatagga attaataaaa
     1921 aatagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
     1981 ttaaccttaa aggccggaga agccagcacc agcccacgaa ttgcaggtgc ataatcatga
     2041 acccatgtgg caacaagcac tgcgccgacg ctttgcgcga tcactaccac ctcttccagt
     2101 ccgacctggc tgtcgctgac agcaaaacgg acaaattcat cgacatcctg cactgagcgc
     2161 ataagagacg ggctataacc acgcggcccc gaattttttc catgcccccg ggcatcccag
     2221 gcataaaaag cagtatcagg catcgccagt tcatcaacga gatgttgtag acgaccagaa
     2281 tgttcatgcc cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagtgggc
     2341 cagtgccgat aatacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
     2401 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
     2461 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctagt
     2521 aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat cactacatcg
     2581 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
     2641 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac gccccccagc
     2701 agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
     2761 ttcccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag aaataacacc
     2821 agtaacgccc cgccctggat atttatggtt ggcaacatta atagccaggc ggcatgactc
     2881 aaggcaaaaa cggtcattat ccatccccaa aaaatggcgc tcacggtatg ctgaaaaccg
     2941 gaggggtcac ccacaaaaac tcgccaggtg gctaatatca gaaacccagt caaaggtata
     3001 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc
     3061 caatataacc aacgcgaaaa atgtacggat ataagcgtac aatattcttt caatgccaga
     3121 aaacttatca gcgtaaaaaa cgtcaacgtc atccagcgtg ggcctgaaat caccagtgaa
     3181 aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc cccttttctc
     3241 tcaggtaacc gccatagcag aaaacggttt atcagcttgc taaaattaac aacgcgaaaa
     3301 gtgttgccag agatttttcc agcattatct ttcagcgctc ataagaagca cactccgaca
     3361 acgattaatc gcagtacaga taaacagaat tgaagcaatg ctccataaaa gattattcca
     3421 ttgcatccac tgtggataaa tggcaacggc cagaccccac agaccaaata ttaacgcgcg
     3481 atcacttttg ccaaatggac cggcataact gcgaacaccg ttaatcgtct gcgcgagtaa
     3541 accgcaaaac tcggtcaata tggtgcaaaa taacatgagt atcacgagtg atgcgttact
     3601 ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg gaaataacat cgccagtctc
     3661 attcaaaatc gcccccagac gtgtttgctg attgcactca cgcgccaaca tgccatccag
     3721 cgcattgagc gccatacgga taaaaagcac gatgggcaat agcaaaaaga ggatgggttg
     3781 tgccaccaac accaacagca atccggtaaa aagagaaagc gccagtgccg taagagtgat
     3841 gtgattcgct gtaacgtggt gcttataaag ccaaaacatc gtcggcctta acagcgactg
     3901 aaagagcggt tttatctggt agagcgtcat tatcaatcct tgataattcg gtttgcgcta
     3961 tggtgcatca attcccctga cggagatatc gcaacacgcc gcttcattaa aaattcagaa
     4021 tatatcaagg tattgggggc tcactgtcag gaatgattat ccccaataaa tcaagtagtt
     4081 aaaaattatt acgctgttat tcaatacaac tcatcaagtt tcctcatcgc ttgttcccgt
     4141 attgcgcgcg tcttaccgtt aagcgccagc tggcggacta aatccagttc agggttaaca
     4201 agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc aactggaaag
     4261 cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc cagtgccgca
     4321 gagggggtgt ggcggttgct aagtgcgccg aacttgaaaa atgtgagatt gcttttcgcc
     4381 agtgtactcc accatatacg atccggttgt tttgccagta catccagttg tgctaacgca
     4441 tcggggtcga gtgcataata aacacgtaat gcttcatcaa cctgcgcacg ggagctattt
     4501 tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg ctgtaattcc
     4561 ttgagaacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa aaagtcccag
     4621 tcttccaaac gtatcagggc ggcggcattt aatagcatct ggtcatcaac ttcgccgtgg
     4681 ctaatcagct cagacaaaat catatcagcg ctggtttcat atgcatctga atctgctttc
     4741 ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat cgacgtgctc
     4801 agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg tgtcaataac
     4861 cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa gtaggcaggg
     4921 gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa tgctaaaaat
     4981 aacacacgta ttagatcatc gtcattctcg aatttttggt ataaataggc tgcaactttg
     5041 tcactctctt gtggcgtcat ccgcaataat tgaccatggt gtttcatctc tgccagcttg
     5101 aacgctaagc ttgtgacaac attgtttgat gaatcgttcg ccaactgcat tgcctgattg
     5161 taagtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact gcggatatag
     5221 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
     5281 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
     5341 agattcaacg gaccgacacc atcaggtaga cgactcattc gcgtcattat ttcgacagag
     5401 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
     5461 aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt ttgttcatca
     5521 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
     5581 cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct
     5641 tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
     5701 tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
     5761 tctttcagag catcttcatc atttattaga ccccaataat atgcatcagg atttattatc
     5821 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
     5881 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatac cccacgcgca
     5941 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
     6001 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
     6061 acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
     6121 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc taccactaaa
     6181 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
     6241 ctgttgatag cttgttcaac ttgttgttt
//
LOCUS       DAEEDS010000153         6256 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEEDS010000153 DAEEDS010000000
VERSION     DAEEDS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.155"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(57..536)
                     /gene="xdhC"
                     /locus_tag="KLH32_003836"
     CDS             complement(57..536)
                     /gene="xdhC"
                     /locus_tag="KLH32_003836"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBE0140480.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(533..1411)
                     /gene="xdhB"
                     /locus_tag="KLH32_003837"
     CDS             complement(533..1411)
                     /gene="xdhB"
                     /locus_tag="KLH32_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBE0140481.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1422..3719)
                     /gene="xdhA"
                     /locus_tag="KLH32_003838"
     CDS             complement(1422..3719)
                     /gene="xdhA"
                     /locus_tag="KLH32_003838"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBE0140482.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4134..4889
                     /locus_tag="KLH32_003839"
     CDS             4134..4889
                     /locus_tag="KLH32_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBE0140483.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4968..5041
                     /locus_tag="KLH32_003840"
     tRNA            4968..5041
                     /locus_tag="KLH32_003840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5000..5002,aa:Gly,seq:ccc)
     gene            5156..>6256
                     /locus_tag="KLH32_003841"
     CDS             5156..>6256
                     /locus_tag="KLH32_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE0140484.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLN"
BASE COUNT         1555 a         1569 c         1620 g         1512 t
ORIGIN      
        1 agttgttaaa cacataacct cagtgaatcc ggcctgaatt caggccggat atccttttac
       61 ttcgttttct cgcaatccag aactgtattt acaatcatct gataccccgt gcagcgacaa
      121 agatttcccg ccagtccgcg acgaatttcc gtaatggtta atggcttctc gcgtggtttc
      181 gccagcattg ccgtggtagc cataatcagg ccaggcgtac aaaacccgca ctgcactgtg
      241 ccggatttcg cataagcctg ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc
      301 agcgtgcgga tctcttttcc ttcagcccag gcggcaaggt ataagcaact gtctattgct
      361 gtgccgtcga ccaacaccgt acaggcacca cattcaccca cgcagcaccc ttgtttgaca
      421 cttagcagtc cttgttcgcg gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc
      481 tgaaaaggca tcccgttaat ggtgcattcg gtggtaattg tttcgctgtg attcattgca
      541 attttccccc cgccgcggcg acggcttcgc taatcacttt tttggtcatc gtctggatga
      601 gatgcagacg aaactcttta ctggcccgcc atgaagaacg cggggcgaca tcttgcagga
      661 cagattcgct gatagcttcc agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt
      721 cggcatgttg gcagcgaatc ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc
      781 tgaaattgcc gttatccagt cggcaatgtg cggcgcagcc aatcgttgaa atatccattg
      841 cgtcgcgcat ggcatattta aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa
      901 aatgaaaggc gacgaggatt tcgtcatgct caagagacac tttgcccggc ccggtgtgaa
      961 agccattaat cgggacgaaa cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat
     1021 aaattagcgt tggcgtggca gaatctgcgc tggtggcacc gttgcaaata tttccaccgt
     1081 aggtagcgac gttacggatc tgcggtccag caatgaacgt ggccgcagca cataacgccg
     1141 ggagatgacg ttgagttata ggatcttcta ttagctgggt aaatgtcgtt gcagagccga
     1201 tacgtagcga gccatcttcc gccagcgtaa ttccccgcag ctccgccaga ttatgaatat
     1261 caacaatatg gcgataacgg tcattgtgat ggtggagctg aatcagtacg tcagtgccac
     1321 cggcgagcag tttggcctgc gggttgtcag ccagcaggtt gatggcatcg gcaagggttg
     1381 ctgcgcgatg gtaagaagca aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga
     1441 tggaactctt catataaccg ttttggcgtc agcggcagtg tattgattgc aacaccggta
     1501 gccatcttca ccgcgttacg aatagcagcg gcaacaggaa ttatgggtgg ctcacccagt
     1561 gacttatgtc cgtatgcgga ttgcggctca ttgatttcga cgaacgcgct ttccagttgt
     1621 ggcagatccg gcatggtcgg cattttgtaa tccagcagat tggggttacg gaccacgccg
     1681 cttttagcat cgatgatcat ctcttcaaat agcgcccagt caatgcccat tcccattccg
     1741 ccgtgtacct gaccttctgc cagcagtgga ttaagaatat gccctgaatc atgaacgttg
     1801 aggatgcggt tgatggtgac tttgcacagc gcaatatcga ccgtcagatc aacaaaggta
     1861 cagccaaacg ccggtgggtt agtggtggtt ttgatggagc tttcagcaga gagctgcccg
     1921 ccgcgttcag ggtggtagaa agcgtccatc gccaaatctt ttaacgacat taacggctct
     1981 tccggtcgtt caaccagcac gatatggcct tttatcaggg tcaaattcat cgctgactga
     2041 tgtagcatga ctgcggcgtg agcgatgatt ttctctttta ataatagtgc cgcactgcgc
     2101 agcgcaggcg cggcaacata gctctggcgt gaggcaaatg cgccgggatc gaacggcgta
     2161 acgtcggtat cttgtgttga aataacgcga acgtcgctga ccggaacccc cacggtttct
     2221 gccaccattt gcgagaagac ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc
     2281 acgttgatgg ttccatcctg attcatcaga aggcgcgcgc ctgctatttc tacgccgaca
     2341 ggccaggtgt tagaggtgta gctaaaacag gcgacgccaa cgccgcggcg caaattgcct
     2401 tgctggttct ggcattctgc acggcgtttt tcccattcaa agattttccg gcctttttca
     2461 agacactccg gcaaccctgc gctgcaaata cgtttgcccg tgagcggatt agcatctcct
     2521 tcgcgggcgg cgttgcgtaa acgaatttca acaggatcaa tacctaacgc tgtcgcggcg
     2581 tcatcaagca tagactcaac ggcaaatacg acttgtggcg cgccataacc acgcatcgca
     2641 ccagccgagg ggaggttggt atagcaggtc tttgaactgt aagcgtaggc acaacgagga
     2701 taaaggtaag cgactttatt cccccccgca gaagcgatgg agtgcccgtg agatgcataa
     2761 gcgccggtgt tagacagaac atccagacta taacctttca aggttccgtc gcggttcacg
     2821 cccatttgcc cgtcaatagt aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca
     2881 cggctaaggg aaactttcac cggaatgccg ccaagcttgc tggtcaggaa tgccgccatt
     2941 ggctcttcca gtacatcctg tttattacca aaaccgccac cgacaaacgg tttgatgact
     3001 cgtacgcatg accagggaat atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg
     3061 atctgggtgc tggaaacgat ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt
     3121 acgctttcca tgtgacaatg ttgaataacg ggagtctgat agtgcccctg tacctggtag
     3181 tcggcggcat cgattgtttg ttggacattg cccgtcgaca tcgtgctttg tttcagtaaa
     3241 ttgccgccgt tatggattgg tgcagcgtct tctgtcagcg ccgcttctgg cgtggtgata
     3301 acgggtaatt cttgccactc aatgctgacc aattgcgccg ctttttctgc cgtgagttca
     3361 tcgcgggcca cgacgatggc aacggcgtca ccatgatgac gaacatggcg agttagcagt
     3421 gcgcgatcgg cggtatcgcg cttgttttcg tcaagtgtcc aggcatgccc agctgtagcg
     3481 aatggaatat caggcacatc ctcccaagta aaaatcgcca gtacgcccgg taaacttctg
     3541 gcttgttcat cattaatact tacggcataa ccatgtgcga tagggctacg tacatatttc
     3601 gcgtaacaca tgcccgccat aacataatcg tcagtatatc gtgcccgccc ggtgacctta
     3661 gcaatggcat cgacgcgcat gcatgattca cccgtagcgg ttgcttcccg cgcttccata
     3721 gaaatcccct cgatttgaac atttggttct tatggcaaag atttagtcta agcaggcaag
     3781 atttacgcca gaaacgacga taagcgttag gggaggaagg aagtgtgatg acgttaaaaa
     3841 aacacagata tgatacgaaa caggactctg aatagggaca gtgatacgta tcaatatgag
     3901 tggcgataaa atcaatgtga taaatcagga ttattacgca tcaggatggg aaatagggta
     3961 aaaagtcagg aacttcgata cctctacagc gaatattttg ctgatgatac ttccctttgc
     4021 caaatgaaat taacctcttt ctctattacc acgtttttcc agaagcaaga gattgggtat
     4081 caagaggctg gctgctatga tatggcgtct tgttttttaa gcgaggaaag attttgagtg
     4141 cgggacgcct gaataaaaaa tctctgggta tcgtgatgtt tttatcggtt ggactgctat
     4201 tggcgggctg ttcgggtagc aaatcatccg atacaggaac gtattccggc tccgtttaca
     4261 ccgtgaaacg gggggatacg ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag
     4321 agctggcgcg actgaacggc atttcccccc cttacaccat tgaagttggt cagaaactaa
     4381 aactgggtgg ggcgaaaagt agcagtagta cacgtaaatc aaccgccaaa tcaacgacta
     4441 aaaccgcatc ggttacaccg tcatcagcgg taccgaaatc ttcctggccg ccagtagggc
     4501 aacgttgttg gttatggcca acgacaggga aagttatcat gccgtattcg acagcagatg
     4561 gcggcaataa agggattgat atctcagctc cacggggtac acctatttac gccgcgggtg
     4621 caggaaaggt ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc atcatgatta
     4681 aacacagtga agattacatt acggcttacg cccataatga cacgatgctg gtaaataatg
     4741 ggcaaagcgt gaaggctggg caaaaaatcg ccactatggg gagcacggat gcggcatctg
     4801 ttcgcctgca tttccagatt cgttaccgtg caacggcaat tgatccgcta cgttacttgc
     4861 cgccgcaggg cagcaagcca aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc
     4921 acttgctaag gagagcgtaa ggtttataat gccttacgca tctcgaagcg ggcgtagttc
     4981 aatggtagaa cgagagcttc ccaagctcta tacgagggtt cgattccctt cgtccgctcc
     5041 atgacctttc ttaaagtact ccaacctact gaatcctaag ggatatggct ttaagcctcc
     5101 ttgcagagtt acttactgta acccactgtg taaccctctc agggcataca gacatatgat
     5161 tcagctatcc agtagtcaca aacttccagc agtctactat ctctaccagc gtaacggcgt
     5221 ctactacttc cgtcttcggg tcagacaatc aaacaatgat agaatgacct cagtatcatt
     5281 acggaccaag gatagacgca ccgctatggc tcactcaagg cacatcaagg cagcactcaa
     5341 ggcaatacac gcagacagac ccaacgctac ctatgaggag atgagggagc accttaagga
     5401 catcgctgag tgtgaactgt ctatgggccg ttcagatctc tttgagcctg acatgaggga
     5461 tatctaccgg gaccaatacg gagaacttgg tgagagcctt accgatgctc tggctagtga
     5521 gccgctaagt attgaccagc atcgttacat caatgaggcc ctgaaggtgc taaaagcctg
     5581 tatgaggcgt atcgaagcgg gagatagtca gcctctgata gattacgtcg atcgttttaa
     5641 tgatattgat cggcaagatg accaagcaga ctctgtgtcc ctatctgtta gtgctccaga
     5701 agtaaaacca gaagttacgc ctagcattac catagcgagc ctctttgagc aatacgaagc
     5761 ggagaactct cagaactgga agcctgcaac cttaagggaa aacaaagcgt cacacgctgc
     5821 gttaatcgaa atattcgacc atctgggtct caatgcagac gctaacaggg ctgacatgct
     5881 taaggtccgt gatgtgctcc agcagttacc taagaaccgt aagcagcgct ttaaggatgc
     5941 cccacttgct gacctgttga gccgtgagga taagacagat tgtctggatg tcgttaccat
     6001 caacaacaag tatcttatta agatggctgc tgtctttagg tgggctgtgc gcaatgactt
     6061 gattaaaaag aacatgactg agggtctgga gcttaaagtc cctcagcgta aagcctcagg
     6121 ggcccgtaac gccttctcta cggagcaggt ggggcaacta ctggtcgcag ctaaggcata
     6181 ctctcagaag acctctggta agccctacca ctattacgtt acggctttgg ctgctatcac
     6241 tggggcgaga ctcaat
//
LOCUS       DAEEDS010000154         6252 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEEDS010000154 DAEEDS010000000
VERSION     DAEEDS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.156"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="KLH32_003842"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="KLH32_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBE0140485.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="KLH32_003843"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="KLH32_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBE0140486.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KLH32_003844"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KLH32_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="KLH32_003845"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="KLH32_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1656 a         1456 c         1476 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag at
//
LOCUS       DAEEDS010000155         6250 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEEDS010000155 DAEEDS010000000
VERSION     DAEEDS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.157"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="KLH32_003846"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="KLH32_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBE0140487.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="KLH32_003847"
     CDS             578..1027
                     /locus_tag="KLH32_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0140488.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="KLH32_003848"
     CDS             complement(1028..1261)
                     /locus_tag="KLH32_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBE0140489.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KLH32_003849"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KLH32_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBE0140490.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="KLH32_003850"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="KLH32_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBE0140491.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="KLH32_003851"
     CDS             complement(2212..3021)
                     /locus_tag="KLH32_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBE0140492.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KLH32_003852"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KLH32_003852"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBE0140493.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="KLH32_003853"
     CDS             complement(4677..5471)
                     /locus_tag="KLH32_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0140494.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="KLH32_003854"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="KLH32_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            5836..6081
                     /locus_tag="KLH32_003855"
                     /pseudo
     CDS             5836..6081
                     /locus_tag="KLH32_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1637 a         1573 c         1419 g         1621 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccattg cggggctgaa tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg
     6061 ctgaggtata tgccggcctg acacaggcta agtgtgcccg ggaatcggtg caagtataaa
     6121 aaacagcctg ttgcggtata agaatcgacc atgcagagag agaaaacacc ggagtggagg
     6181 gaaaagcaga aaagctcacg tggcataagg cgtggacagc ggtatcgtct ggtttttcaa
     6241 tttcctatac
//
LOCUS       DAEEDS010000156         6239 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEEDS010000156 DAEEDS010000000
VERSION     DAEEDS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.158"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            393..2051
                     /locus_tag="KLH32_003856"
     CDS             393..2051
                     /locus_tag="KLH32_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBE0140495.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="KLH32_003857"
     CDS             complement(2048..3004)
                     /locus_tag="KLH32_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBE0140496.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="KLH32_003858"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="KLH32_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBE0140497.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="KLH32_003859"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="KLH32_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBE0140498.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1469 a         1481 c         1736 g         1553 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgat
//
LOCUS       DAEEDS010000157         6215 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEEDS010000157 DAEEDS010000000
VERSION     DAEEDS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.159"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..378
                     /locus_tag="KLH32_003860"
     CDS             <1..378
                     /locus_tag="KLH32_003860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140499.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="KLH32_003861"
     CDS             complement(454..918)
                     /locus_tag="KLH32_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBE0140500.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KLH32_003862"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KLH32_003862"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBE0140501.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="KLH32_003863"
     CDS             complement(1787..2476)
                     /locus_tag="KLH32_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBE0140502.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KLH32_003864"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KLH32_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBE0140503.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KLH32_003865"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KLH32_003865"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBE0140504.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KLH32_003866"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KLH32_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBE0140505.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1576 a         1689 c         1547 g         1403 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatc
//
LOCUS       DAEEDS010000158         6210 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEEDS010000158 DAEEDS010000000
VERSION     DAEEDS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.160"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..95)
                     /locus_tag="KLH32_003867"
     CDS             complement(<1..95)
                     /locus_tag="KLH32_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140506.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYL"
     gene            586..1494
                     /locus_tag="KLH32_003868"
     CDS             586..1494
                     /locus_tag="KLH32_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBE0140507.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1540..3531)
                     /gene="tkt"
                     /locus_tag="KLH32_003869"
     CDS             complement(1540..3531)
                     /gene="tkt"
                     /locus_tag="KLH32_003869"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE0140508.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3809..4567
                     /gene="loiP"
                     /locus_tag="KLH32_003870"
                     /gene_synonym="yggG"
     CDS             3809..4567
                     /gene="loiP"
                     /locus_tag="KLH32_003870"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBE0140509.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4773..5693)
                     /gene="speB"
                     /locus_tag="KLH32_003871"
     CDS             complement(4773..5693)
                     /gene="speB"
                     /locus_tag="KLH32_003871"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBE0140510.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5829..>6210)
                     /locus_tag="KLH32_003872"
     CDS             complement(5829..>6210)
                     /locus_tag="KLH32_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140511.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1535 a         1616 c         1599 g         1460 t
ORIGIN      
        1 cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg
       61 gagcaggaag gacatctgat ggaaatggaa gccacgcaag caccttaaaa tcaccatcat
      121 acactaaatc agtaagttgg cagcattacc cttttgctga atgtataact gagattgatg
      181 attctggaaa ataatcacta agccgcataa attatcctta ttttattttt agggtaccgt
      241 ccggcggtaa aaataaaata ctcgtgcggt ttaatgaaag caattgggta cgcgagaaga
      301 aaaaccccac aaaccaaaat aataaaacct taaataacaa caatatgaaa ttgatatttt
      361 taaagtcaac tttgtatttg ttatgttcat cacaaaatca acattttgtt tgttattgta
      421 tttgtgatgc atatctctat ttatttattg aatgttcgaa aacatacttt tctaatgcaa
      481 tgttttttcg ctatacccct ttatagattc tgaattctca cctgtcggct gttttctcct
      541 catctcatgt gacatattcc catactttac tcaaggaaga ttgtcatgaa agcacctgtt
      601 tcattaacct catttcgccc acaaaaatct cttgctatcg ccattggcgt gcttgccgtg
      661 gttgtgctgc catttctctc gtattacacc gttaatgaag gtgaacgcgg cattttgctg
      721 cgctacggaa aaatcgtaaa agttgccgaa ccggggctgg gttttaagat cccttttatg
      781 gaaagcgtgg agaaaatttc cacgcgtaat caggctgtgg tctatcaggg attacaggcc
      841 tacagccgcg accagcaacc ggcacaaatg acagtatcag tcagcttcca cattaaacct
      901 tcagaagcag gcgcggttta taccacctac aacactattg aagcattaaa agatcgtcta
      961 attgtccgtc agttgccaac tcagctggag aacattttcg gccaatatac cgccatcagc
     1021 gccgtacagg atcgtacaaa attggttcag gatctgcaaa atgccatgcg caaagccgtt
     1081 gttgggccag tggtaattga cggggtccag attgaaaata ttgatttttc tgacgcttat
     1141 gaaaagtcga ttgaaaaccg aatgaaagca gaagtcgcca ttgccacgcg taagcaaaat
     1201 ctggaaacag agaaaatcca ggcgcaaata gcggtaactc aggcccaggc agaagcagac
     1261 agtaaactgg cggcagccaa agtagaagcg gaaactattc gcgtaagagg cgcggcagag
     1321 gctgaaacta ttcgtttaaa aagtgcggct gaagcagaag caatccgcct gcgcggtgaa
     1381 gctctgcggg ataatcccgg acttgtagcc ctcaccactg ccgaacgttg ggacggtaaa
     1441 ctgcccgata ccatgatccc cggcagcaca gttccgttta tctctactaa gtaaaaaaag
     1501 ggccgctttc gcggtccttc tcacaaaacg tcttactgat tacagcagcg cttttgcttt
     1561 cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac agcagctctg ccggagcaga
     1621 ttcaccgaag gtggtcatgc cgacgatagc gccgttcagg ccaacatact tgtaccagta
     1681 gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc gctttcggca gtacggattc
     1741 acggtaagcc gcatcctgct tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc
     1801 tttcacgcct tcggcagtca gtttttcgta ggcagcaacc gccagttcaa cttcagaacc
     1861 ggtggcgatg aagatcagtt ccggctgacc agcacagtct ttcagcacat aaccaccgcg
     1921 cgcgatgttt gccagctgct cttcagtacg ttcctgctgc gccaggttct gacgggagag
     1981 gataagcgca gtcgggccgt cctgacgctc aacgccgtat ttccacgcga ccgcggattc
     2041 aacctggtca cacggacgcc atgtagacat gttcggggtc acgcgcagag aagcgacctg
     2101 ctctaccggc tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac
     2161 catcacctgg cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattccac
     2221 gaacatcagg aaggtggagg tgtacggcag gaagccaccg tgcagggaga taccgttagc
     2281 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacctg cagcatcttc
     2341 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcagagcc
     2401 gccgaggaat tcaggcaaca gcgggccgaa cgcttcgata gcattctgcg acgctttacg
     2461 gctggcgatt ttcgccggat tagcctgcag tttagcgata aactctttcg ctttggcgtc
     2521 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagccgctt cctgcggata
     2581 agctttcgcg taagccgcaa acttctcatt ccatgcagat tctttcgcct ggcctgcttc
     2641 tttcgcatcc cactgagcat agatttcaga cgggatttcg aacggtgcat atttccagcc
     2701 cagctgttcg cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg
     2761 agtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     2821 tttgtcagtc actgcgcgcg cttcttcaac tgcgcgtttg atggatgccg cgtcatgacc
     2881 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgctg tgtcgtcggt
     2941 gaaccagcct tcaacgtgac catcgataga aataccgttg tcatcgtaga acgcaatcag
     3001 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     3061 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt
     3121 aaactgcgcc gccagcgttt tttctgcaat cgccataccg actgcgttgg caataccctg
     3181 acccagcgga ccggtggtgg tttccacacc agcggtataa cctacttccg ggtggcccgg
     3241 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     3301 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     3361 acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc
     3421 ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc
     3481 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     3541 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     3601 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     3661 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctgctcgc ccgtccgctg
     3721 cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat
     3781 aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag
     3841 cgtggcaacg gtactgaccg gttgccagaa tatggactcc aacggactgc tctcatcagg
     3901 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     3961 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     4021 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta
     4081 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt
     4141 ctatagcggg ctgatggata tgatgacaga taacgaagtc gaagcggtga tcggtcacga
     4201 gatggggcac gtggcgttag gccatgtgaa gaaagggatg caggtggcgc ttggtacaaa
     4261 tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggcagtttat ctcaatcaca
     4321 acttggtgat ctgggcgaga aattagtcaa ttcgcaattc tcccagcgtc aggaagcaga
     4381 agccgatgat tattcttacg atcttctgca ccaacgcggc atcagcccgg caggtcttgc
     4441 caccagcttt gaaaaactgg cgaagctgga agaaggccgt caaagctcaa tgtttgacga
     4501 ccatcctgca tccgccgaac gtgcccagca tattcgcgat cgcatcagcg cggatgggat
     4561 taagtaaagc ctagtggtgt cgaacgtagg tcagataagg cgttcacgcc gcatctgaca
     4621 ttaatggcac atttgttacc ttgtgcgcat tgccggatgc gatgctggcg catcttatcc
     4681 ggcctacggg tgccgaacgt aggtcggata aggcgttcac gccgcatccg acattaatgg
     4741 cacgttttac ccgtgcgcat cgcatctggt gcttactcgc cctttttcgc cgcctgaata
     4801 tacagcatct ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat
     4861 gccggagcca cttccactac gtccatccca acgatgttga gatctttcag gccgcgtacc
     4921 agtttaatgg cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt tcccggcgcg
     4981 aaagcaggat ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc
     5041 tgtttcacct gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc
     5101 acggtaaagc cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc
     5161 gggtcgatca gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa
     5221 ccgttcgcat aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc
     5281 ttcgcatgag cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggagagcata
     5341 cgcttaccgg cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg
     5401 gcatcgccaa aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg
     5461 tcgaaattcc acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc
     5521 gctgccggac cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca
     5581 gtaatcaccc agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa
     5641 ccaaaggcat tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga
     5701 ctcctattac aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg
     5761 gttggatttg cgaaacacaa gtttgtgcta attatcgcct taaatgggcg aggcatcaag
     5821 cgaggttgct atttccgaca cggtggcggc gcatagccat tgttatctgc cggaaaggct
     5881 tgtccagagc acggcggcag cgcgttttgc gccgggcctt gtaattcaaa ggcggcaaag
     5941 ccgattaccc cggtattacg cacatcgcct tgcgccgaat tggctgcgta ggaatcagca
     6001 acgttggcga actgaaaagc cgcttcagta tgtttatctt tgcggaatcc tttaatcact
     6061 aacgaatctc cggcattcac aatatagccg ttgttattca gcgaaccttg cttgccgttc
     6121 agcacatcca ggccatcagc cgtagcaacg atctcgtagt tggtgttacg actgtaatta
     6181 cggacatata attgataatt cattccgact
//
LOCUS       DAEEDS010000159         6059 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEEDS010000159 DAEEDS010000000
VERSION     DAEEDS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.161"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            382..1410
                     /gene="torT"
                     /locus_tag="KLH32_003873"
     CDS             382..1410
                     /gene="torT"
                     /locus_tag="KLH32_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBE0140512.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1383..2075)
                     /gene="torR"
                     /locus_tag="KLH32_003874"
     CDS             complement(1383..2075)
                     /gene="torR"
                     /locus_tag="KLH32_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBE0140513.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2162..3334
                     /gene="torC"
                     /locus_tag="KLH32_003875"
     CDS             2162..3334
                     /gene="torC"
                     /locus_tag="KLH32_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBE0140514.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3334..5874
                     /gene="torA"
                     /locus_tag="KLH32_003876"
     CDS             3334..5874
                     /gene="torA"
                     /locus_tag="KLH32_003876"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBE0140515.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5871..>6059
                     /gene="torD"
                     /locus_tag="KLH32_003877"
     CDS             5871..>6059
                     /gene="torD"
                     /locus_tag="KLH32_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBE0140516.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1471 a         1516 c         1680 g         1392 t
ORIGIN      
        1 tccatttcgg tcccagttag gacgctttct catcaagcca gtccgcccac cactgcatca
       61 tctccctacg cttatcgaga tactgagcat ggttgtaaat tcaattaacc cagtgcgttg
      121 acaaaaaatt agcgcaagaa gacaaaaatc accttgcgct aatgctctgt tacaggtcac
      181 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg tcgcgttttt
      241 cagtctatta tttaatcatt aagtaatcta atgctatgat ttttaaatta tttatgtgat
      301 tttgtcatcc tttaggtgaa aaaggttgag tcgcaaagtg gaatgcatct agcataaagc
      361 cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc ccttttcatg
      421 ttgtcggcat tttcggctga taacctgttg cgctggcatg atgcgcagca tttcacggtg
      481 caagcctcta tgccgcttaa agccaaacgc gtatggaaac tgtgcgcgct ttatcccagc
      541 ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc tcgccgctac
      601 ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac ccagcaagca
      661 caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag tagcacgacg
      721 tcatttcccg acctgcaaaa gcaggtagca aatctgccgg tgatcgaact ggtaaatgct
      781 cttgatgatc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa
      841 cctgggcgat atttggtgca atgggcgcac ggtaaaccac tgaacgtgct gctgatgccc
      901 ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc agccattgcc
      961 ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga aatccagcgc
     1021 aacctgttgc aggagatgct ggaacgccac ccagaaatcg acgtcgttgc cggaacggcc
     1081 attgcggcag aggcggcaat gggtgaaggg cgtaacctga aaacgccgct taccgtggtg
     1141 tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt gattatggct
     1201 gccagcgatc aaatggtctg gcagggggaa ctggcagttg agcaggccat caggcaatta
     1261 caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac gccgaaaaat
     1321 gccgaccgag aacatatccg ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt
     1381 tatcagcaca catcagcggc taagaaataa ccttcaccat gttgcgtcac cagtaaatcc
     1441 gcgctgagtt tatgacgtaa acgacgaatt aacacatcga cggtgcgcag gtcagggttt
     1501 tccacccgac gcgcagaaag catacgcagc agacgctcac ggctgagaat ttcgcccgga
     1561 ttagtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat cggctcgcca
     1621 tcccgctcca gcgtatggcg cgacacattc aggcaataac ccgcaaagcg atagcagtag
     1681 tcctgagtgt gcggttgagc ttgtcgcgcg aggtcgattc gccagagcag atttttcacc
     1741 cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc
     1801 agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt
     1861 gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggtaa gttgatatcc
     1921 agcagaatta aatctaccgg ctgattctgc ataatttccc gtagcccggc accgctcgcc
     1981 gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaacc
     2041 ggcttatctt caacaataac aatgtgatgt ggcatcagag ggttttactc attctgttca
     2101 tatctgttca tatttgctca ttaagatcgc ttcactaaac cataattcta caggggttat
     2161 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
     2221 ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
     2281 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
     2341 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gccatgactg
     2401 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaacgacat
     2461 ttaccagacc tttattgccc actccattga tacacctgaa aaattcgaag ccaaacgcgc
     2521 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgtcgctc
     2581 ctgccataac tacgatgcga tggatcatgc caggcagcat cctgaagcgg cacgtcagat
     2641 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
     2701 gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
     2761 tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg cgaaaggcga
     2821 taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
     2881 cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc agcgtgtact
     2941 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
     3001 ggtgaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
     3061 ggccaccgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggccta
     3121 tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatcgccca
     3181 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagtctcga
     3241 taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
     3301 taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
     3361 tcacgtcggc gttttctggc acaacttggc ggcttaaccg tcgccgggat gctggggccg
     3421 tcattgttaa cgccgcgccg tgcaactgcg gcgcaagcgg cgactgaggc tgtcatctcg
     3481 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgatggt gaaggatggt
     3541 cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat gatcgccgga
     3601 ttgccggatc acgtacacaa cgcggcacgt attcgttatc cgatggtacg cgtggactgg
     3661 ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
     3721 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca gaaaactcac
     3781 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
     3841 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat
     3901 tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcaat ggaagtgtat
     3961 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
     4021 tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
     4081 tattacgagc agctaaaagc gaaagtcgcc gccggtgaag ttgaggtcat cagcatcgat
     4141 ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
     4201 aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcac atacgctgta cagtgaaaac
     4261 ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt cctgccgtat
     4321 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc
     4381 attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag aacgcaaatt
     4441 attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg
     4501 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ctggcactac
     4561 aacggcgcag gcacgccggg gcgtaaaggc gttattctga gtggtttctc cggctctacg
     4621 tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac tattccgatt
     4681 gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa cggtaaatcg
     4741 gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccatt ccatcgccat
     4801 cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat cgccatagat
     4861 aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc tgcctgcgac cacgcagttt
     4921 gagcgtaacg atctcgacca gtacggcaac cactccaacc gtggcattat cgccatgaaa
     4981 caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg cgagctgtgc
     5041 cgtcgcttta atcgtgaaga agcctttacc gaagggctgg acgaaatggg ctggctgaaa
     5101 cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct gccagcgttt
     5161 gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat gtttgttcgc
     5221 caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag tggcctgatt
     5281 gagatctact cgaaaaccat cgccgatatg aactacgacg attgtcaggg gcatccgatg
     5341 tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta tccgttgcat
     5401 ctgcaatctg tgcatccgga tttccgactt cactcgcagc tatgtgagtc ggaaacgctg
     5461 cgtcagcaat atacggtagc gggtaaagag ccagtgttca ttaacccgca ggatgccagc
     5521 gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg
     5581 gcaggagcag tagtttctga ccgctatgca cccggcgtgg cgcgaattca cgaaggggca
     5641 tggtacgatc cagataaagg cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac
     5701 gtgctgacca tcgacatcgg gacttcgcag ctggcgcagg cggccagtgc gcacactacg
     5761 ctggtggaaa ttgagaagta caatggaaca gtggagcagg tgacggcgtt taacggcccc
     5821 gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc aggtgaaatc atgaccacgc
     5881 tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc tcccgtgaac
     5941 tggacgatga acaactgacg caaatcgcca gtgcgcagat ggctgaatgg ttttcgttgc
     6001 tgaaaagcga accgccgctc actgcggcgg tgaatgatct ggaaaacagt attgccacg
//
LOCUS       DAEEDS010000160         6034 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEEDS010000160 DAEEDS010000000
VERSION     DAEEDS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.162"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            92..868
                     /gene="exuR"
                     /locus_tag="KLH32_003878"
     CDS             92..868
                     /gene="exuR"
                     /locus_tag="KLH32_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBE0140517.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1213..1875
                     /locus_tag="KLH32_003879"
     CDS             1213..1875
                     /locus_tag="KLH32_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0140518.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1879..2262
                     /gene="mzrA"
                     /locus_tag="KLH32_003880"
     CDS             1879..2262
                     /gene="mzrA"
                     /locus_tag="KLH32_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBE0140519.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2409..2763
                     /locus_tag="KLH32_003881"
                     /pseudo
     CDS             2409..2763
                     /locus_tag="KLH32_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2801..3106
                     /locus_tag="KLH32_003882"
     CDS             2801..3106
                     /locus_tag="KLH32_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBE0140520.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3109..3513
                     /locus_tag="KLH32_003883"
     CDS             3109..3513
                     /locus_tag="KLH32_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBE0140521.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3503..3802
                     /locus_tag="KLH32_003884"
     CDS             3503..3802
                     /locus_tag="KLH32_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBE0140522.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3988..4284
                     /locus_tag="KLH32_003885"
     CDS             3988..4284
                     /locus_tag="KLH32_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE0140523.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4354..5340
                     /gene="yqjG"
                     /locus_tag="KLH32_003886"
     CDS             4354..5340
                     /gene="yqjG"
                     /locus_tag="KLH32_003886"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBE0140524.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5633..6001
                     /locus_tag="KLH32_003887"
     CDS             5633..6001
                     /locus_tag="KLH32_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBE0140525.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1527 a         1471 c         1519 g         1517 t
ORIGIN      
        1 attcaatcat caaatttata tatgaccaat ggcataccca tgaataaagt ggtatgataa
       61 cttcgtccgc attccactcc gggagacgca tatggaaatc actgaaccac gccgtttgta
      121 tcaacaactt gctgctgacc tgaaagagcg cattgaacag ggcgtctatc tggtgggtga
      181 taaactgcct gccgaacgct ttattgccga tgaaaagaac gtcagccgaa cggtagttcg
      241 tgaagccatc atcatgctgg aagttgaagg ctatgtggag gtgcgtaaag gttcaggtat
      301 tcatgtggtt tccaaccagc cgcgtcatca gcaggcggct gacaataata tggaattcgc
      361 caattacggt ccgtttgagt tgcttcaggc tcgccagctc atcgaaagta atattgccga
      421 gttcgcggca actcaggtaa cgaaacaaga catcatgaaa ctgatggcca ttcaggaaca
      481 ggcgcgcggc gaacaatgct tccgtgattc cgagtgggat ttacagttcc acattcaggt
      541 agccctggcg acgcagaact ccgccctggc ggccatcgtt gaaaaaatgt ggacccagcg
      601 tagccataac ccgtactgga aaaaactaca cgaacacatt gattcccgta ccgttgataa
      661 ctggtgtgat gaccacgatc aaattctcaa ggcgttaatt cgcaaagatc ctcatgctgc
      721 taagctggca atgtggcagc atctggaaaa caccaaaatc atgttattta acgaaaccag
      781 cgacgatttc gagttcaatg ccgaccgcta tctgttcgcc gaaaacccgg tagtgcatct
      841 cgatacggcc accagcggca gcaaatgaaa ttcctgtccg acaggcgctg caatgcgcct
      901 gtttgtctcg tcggtaagcg aaaagtataa agtgtcagcc tgtgtaaatc ctctcgccac
      961 cctcccctgc aatcagcaaa atcagactcc acggacatgt aattttgata acgaacaacg
     1021 ttgacctttg ttacaattag attcaatttg aatttatgct tttgaatgct ttcttatctc
     1081 acgttttaac agggaatagt tcaggctgtg ttgatgtatc aaacccgcag aacataccaa
     1141 aacagcaata acattgcggt agtgcatctt ttaaaaccag cgtggcgtta accgattcac
     1201 caggaataat gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt
     1261 gaaaccctgg ccaatccatc gatgattggc atgttgtatt ttgtcttgtt tgtaattttg
     1321 ttccttgaaa atggcttgct tccggcggcc tttttaccgg gcgacagttt actggtattg
     1381 gtcggcgtgt tgattgcgaa aggcgcgatg ggttacccgc aaacgattct gctgctgacc
     1441 gttgccgcca gcctcggttg ctgggtcagc tatattcagg ggcgatggct gggcaatacc
     1501 cgcaccgtac aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat
     1561 ctttttcata aacacggttt atcggcgctg ttaattggtc gctttattgc atttgtcaga
     1621 acactgctgc cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagttcttc
     1681 aactggatga gcggtctgct gtgggtattg atcctgacaa ctctgggtta catgctcggc
     1741 aaaacgccgg tatttttaaa gtacgaggac cagctgatgt catgcctgat gctgctcccg
     1801 gtggtgctgc tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat
     1861 ggaaatcggg ggtaagggat gcaaatacct cgcatgtcgc ttcgccagct agcctggtcc
     1921 ggcactgttt tacttctggt cggcacgctg ttgctggcct ggtccgcggt tcgccagcaa
     1981 gagtctacgc tggcgattcg tgccgttcat caaggcacaa cgatgccaga cggtttttcg
     2041 atctggcatc accttgacgc tcatggcatt cctttcaaaa gtatcacccc caaaaacgac
     2101 actctgttaa ttacatttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac
     2161 agaacattgc cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag
     2221 tggctgaccc ggttacggga taactctcat cgcttcggat aacttcctgt tatccgaaac
     2281 atatcactca ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac
     2341 actaccgctt cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag
     2401 gttcaagaat gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta
     2461 gttatgccac taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg
     2521 ccgaaaaaca ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc
     2581 gggccaactg ttcagatagc cagctgcgtg ccgatcatca caagaaaatc gcaaagcaga
     2641 aagatgaggt ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgccg
     2701 atagaacgga aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac
     2761 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
     2821 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
     2881 atctggcgag aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
     2941 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt
     3001 cgcggcggcg cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg
     3061 cgctgcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
     3121 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
     3181 atggttgaaa tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
     3241 gctaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
     3301 cttatgagcc tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg
     3361 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta
     3421 cgtaaatcgc gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg
     3481 caactgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
     3541 actgcttagc cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct
     3601 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc
     3661 gctggttggc agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg
     3721 ctgggccaga cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa
     3781 gcagcaacag cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca
     3841 aattttttga atatttttga cagttttcct tgctaacaat catcattcac cacgtttatg
     3901 attctctcca tcgacagcaa cgacgctaat agcgcgccct tgcacaaaaa aacaatcagc
     3961 agcctgagtg gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta
     4021 gcgcacattt taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac
     4081 tctggtaccc aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gctgacggca
     4141 tttttatttc acagcaactt tgctgaaggc gtcaactcgc tgatgttcat gaaaaacctg
     4201 acaatttctg gcggattcct gctgctggca attaccggtc cgggcgcgta tagcatcgac
     4261 cgcctgctga ataaaaagtg gtaagcacgc gctatactta acgataaaaa acgaggagga
     4321 agctcctcgt ttttgctatt ggaggagaga aaaatgggtc aactgattga cggcgtctgg
     4381 catgacacct ggtacgatac caaatctacc ggcggtaaat ttcaacgttc agcttccgca
     4441 tttcgtaact ggctcactgc cgatggcgca cctggcccca ctggcacagg cggttttatc
     4501 gcagagaaag atcgttatca tctctatgtt tcactcgcct gcccgtgggc gcaccgcacg
     4561 ctgatcatgc gcaaactcaa aggactggaa ccgtttattt ccgtttccgt agtgaacccg
     4621 ctgatgctgg aaaacggctg gacctttgat gacagttttc cgggagcaac cggagatacg
     4681 ctctatcaac atgaatttct gtatcagctt tatctccacg ccgatccaca ctacagcgga
     4741 cgagttactg ttcccgtgct gtgggacaaa aagaaccaca ccatcgtcag caacgaatca
     4801 gcagaaatca tccgcatgtt caataccgcg tttgatgcgc tgggcgcaaa agcgggtgat
     4861 tactacccac cagccctgca aacgaaaatt gacgaactta acggctggat ttatgacacc
     4921 gttaacaacg gcgtgtataa agctggtttt gccaccagcc agcaagccta cgacgaggcg
     4981 gtggcgaaag tgtttgaatc gctggcgcga ctggagcaga ttttaggcca gcatcgttac
     5041 ctgaccggca accagctaac cgaagccgat attcgcctgt ggactacgct ggtgcgtttt
     5101 gatccagtgt atgtgaccca cttcaagtgt gataagcacc gcatcagcga ttacctgaat
     5161 ctgtatggct tcctgcgcga tatctaccag atgccgggga tcgccgaaac tgtcaatttc
     5221 gatcacatcc gtaatcatta cttccgcagc cataagacca tcaaccctac ggggattatt
     5281 tcaattggtc cgtggcagga tctcgatgaa ccgcatggac gagatgttcg cttcggttaa
     5341 aaaaaggcac cttacgaggt gcctctgata attagctaag tgcatgttgt tcatgtcgga
     5401 tgcggcgtga acgccttatc aaacctacaa agcgtttata ttcaatatgt tacaatgacc
     5461 gcgtaggcca gataagcgta gcgcatctgg cgattttgcg cttgccatca gtcttagcgc
     5521 gccttcacgg cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct
     5581 attcttaatt tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg
     5641 gtatctgaaa gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta
     5701 ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctgc tggataaaat
     5761 gttaggctgg caacgcgctg gtggcgaagg catcctgacg ataatctacg gtattctggt
     5821 gtttttaccg tggtgggcgg ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg
     5881 gtgggcactg ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg
     5941 ccaggcgggt acgccaggcg aaaaccgctt tggaccagat ccaaaactgg aacaagaata
     6001 atgattaagc accttgtaaa aggtgcttca aatt
//
LOCUS       DAEEDS010000161         5985 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEEDS010000161 DAEEDS010000000
VERSION     DAEEDS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.163"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..264)
                     /locus_tag="KLH32_003888"
     CDS             complement(<1..264)
                     /locus_tag="KLH32_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBE0140526.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVI"
     gene            485..913
                     /gene="uspG"
                     /locus_tag="KLH32_003889"
     CDS             485..913
                     /gene="uspG"
                     /locus_tag="KLH32_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBE0140527.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1034..2599)
                     /gene="ahpF"
                     /locus_tag="KLH32_003890"
     CDS             complement(1034..2599)
                     /gene="ahpF"
                     /locus_tag="KLH32_003890"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBE0140528.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2844..3407)
                     /gene="ahpC"
                     /locus_tag="KLH32_003891"
     CDS             complement(2844..3407)
                     /gene="ahpC"
                     /locus_tag="KLH32_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBE0140529.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3779..4525
                     /gene="dsbG"
                     /locus_tag="KLH32_003892"
     CDS             3779..4525
                     /gene="dsbG"
                     /locus_tag="KLH32_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBE0140530.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4735..5637
                     /locus_tag="KLH32_003893"
     CDS             4735..5637
                     /locus_tag="KLH32_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0140531.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5784..5985
                     /locus_tag="KLH32_003894"
                     /pseudo
     CDS             5784..5985
                     /locus_tag="KLH32_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1607 a         1441 c         1348 g         1589 t
ORIGIN      
        1 aatgacgaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt ccttcccggt
       61 ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat
      121 tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttaccgtaa tacgcagaat
      181 aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg
      241 gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct
      301 ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattccta
      361 gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga accagacaaa
      421 tatcagggat aaatattata atcatggaga atgatttgtt catgattaac agggagaaag
      481 ggttatgtat aaggcaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa
      541 agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt
      601 actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga
      661 agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat
      721 cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga
      781 gttggcagaa gaactggggg ctgatgttgt agttatcggt tctcgcaacc catcgatttc
      841 gacccatctg ttaggttcta acgcctcgag cgtgatccgc cacgccaatc tgcctgtact
      901 ggtcgttcgc taacagataa aagtatttat gccgccctgc ctggaacatc ccggcagggc
      961 tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga
     1021 atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg
     1081 ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac
     1141 ccgctgcgaa cacgcctttg acgttggttt cacatttcgc atcaatgata atctcgccca
     1201 tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgga
     1261 caaaaatacc ggccagttcg atgttgtgaa tattgccgct gacacgatct cgatattcca
     1321 gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt
     1381 cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg
     1441 caaattccag cagcgttacg tgctcaacga tacccgccag gtcgattgcc gcttccacgc
     1501 cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggt ccgtcgcagt
     1561 gcgggcagta ggtcacgcct ttagtgcgat actgatcttc gcccggaacg ttcatgttgc
     1621 gccattttgc acccgtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg
     1681 tttcaatctg gtgtagacca ccttcaaccg ctgccgggat cagtttgctg gcgctctggc
     1741 tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccaacttct
     1801 gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac
     1861 caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg
     1921 ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca cgcttgttca
     1981 gctcttctgc cgcacgtttt tccgcgccag tatcaatttt ggcaacgatt tcagtcaacg
     2041 tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt
     2101 tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg
     2161 ggttcaatac gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta tggcaagaga
     2221 gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag
     2281 actgcgcttc tttcgacgga tgaccaccgg tccacagcaa cgccagtacc agcgaggtga
     2341 actcgtggcc cagcggggag cctgcaaaac gtggtccctg gttggaacct gggttggtga
     2401 tcaggaaaga cggcttacgc actggcaagc tgttatcttc tttaaaggtg actttgtctg
     2461 acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca
     2521 gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt
     2581 tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct
     2641 ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc
     2701 acccgaagaa ttaggtgaat ttccgaagta tttcgcgtca gagcaaggcg actagcctgt
     2761 gaatccccgg gagcttacac aagtaagtga ccggggtgag cgggcgacgc caacgccgct
     2821 atggcgtgaa agacgaagga aatttagatt ttaccaacca ggtccagaga cggagccaga
     2881 gttgcttcac cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac
     2941 tgagctgctt tgattttacg cagcagatca gacgcgtcac ggccaatgcc ttcagcggta
     3001 acttcgattg cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga
     3061 ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc
     3121 atcgcatatt tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag
     3181 tgagtatcgg tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg
     3241 tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag
     3301 aagacgctcc agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg
     3361 aatgcctggt ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc
     3421 gtgttttcga tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt
     3481 atcaaatcga taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg
     3541 ccagcggcga taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc
     3601 ctttggtatc ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg
     3661 acatggatta caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat
     3721 gacagtgata ggttatgcct tttactcgac ttttgcactg actgaaaaga acaaattaat
     3781 gttaaaaaag atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc
     3841 ttccccagta aaagcgattg aaaaacaggg cattacaatc atcaaaacgt tcgatgcccc
     3901 cggaggaatg aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac
     3961 tcccgatggt aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag
     4021 taacacactt atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat
     4081 ggaacaatcc cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtctt
     4141 ctccgatccg ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga
     4201 ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc
     4261 gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatatgaagc
     4321 ctctggttgc aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt
     4381 gttaagtgac aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta
     4441 ttacatgagt aaggaaaata cgctgcaaca ggccgtgggg ttgcccgatc agaaaacgct
     4501 taatatcatt atgggaaata aataaaaggt aattcatgga tggttgaact atatccctgc
     4561 ttttcaacca tcctaaaaaa gcatgaattt ctcgctctac cctaagtggc tgtgctattt
     4621 tagaaaaact taatcctgag ttaagcaaaa aaacataatc aataaaaata catacgcaaa
     4681 aaacattgac taagtgaata taccatggaa gaaaaatata aaccggagta gtgtatggcc
     4741 aatctctacg acttgaaaaa gttcgactta aatcttcttg tcatatttga gtgtatttac
     4801 cagcatttaa gcattagcaa agctgctgaa tcactatata tcaccccatc agccgtgagc
     4861 cagtcgttac aacgcttacg tgcgcaattt aatgaccctt tattcattcg ctcaggaaaa
     4921 ggtatcgcgc cgacgacaac agggttgaat ctgcatcacc atcttgagaa aaacctcaaa
     4981 ggccttgaac aaacaattaa tatagtcaat aagtccgaac taaagaaaaa ctttattatc
     5041 tacggccctc agctcatttc ttgctctaat aatagtatgc tgatccgctg cctacggcaa
     5101 gatgcctctg tcgagattga gtgccatgat atcctcatgt cagcagagaa tgctgaagaa
     5161 ctgttggttc accgcaaagc cgatctggtg atcacccaaa tgccagtgat cagccgttcc
     5221 gtcatttgca tgccattgca tacaattcgc aatactctaa tttgtagtaa taagcatccg
     5281 cgaattactg acaatagcac ttatgaacaa attatggcag aagagttcac ccaattaata
     5341 gccaaatcag caggagttga tgatattcaa atggaaatag acgagaagtt tatgaatcgc
     5401 aaaatttcat ttcgtggttc ctctttgctg acaataatta atagcattgc agttacagat
     5461 ttactgggaa tagtccccta tgaactttat aactaccacc gtgactttct taatttaaaa
     5521 gagataaaac tagaacatcc gttaccttca ataaagcttt acatttccta taataaatct
     5581 tctttaaata atttggtgtt ctcccgattt attgaccgtc taaatgagag tttttgatat
     5641 ataactatag acagctacta taactttcat ctatttattc acagcgcgaa tgaaaaataa
     5701 aacactctgc tttacagtgt ttactttata attaacagtc ttatctcact gttcaccctg
     5761 taacgttcag agagcgtctt cagatgtcta tttataaaat tcctcttccg ctcaatattc
     5821 tggaagctgc acgagagcgt attacctaga cactcaacac cctaccccgc gtttgtgtct
     5881 cattttctgg cggcaaagac tctggtttaa tgctgcatct gacagccgaa cttgcccgac
     5941 aaatgggcaa aaaaatctgc gttttgttta tcaactggga ggcgc
//
LOCUS       DAEEDS010000162         5868 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEEDS010000162 DAEEDS010000000
VERSION     DAEEDS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.165"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            11..670
                     /locus_tag="KLH32_003895"
     CDS             11..670
                     /locus_tag="KLH32_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBE0140532.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(871..1248)
                     /locus_tag="KLH32_003896"
     CDS             complement(871..1248)
                     /locus_tag="KLH32_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0140533.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1315..4281)
                     /locus_tag="KLH32_003897"
     CDS             complement(1315..4281)
                     /locus_tag="KLH32_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBE0140534.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4284..4844)
                     /locus_tag="KLH32_003898"
     CDS             complement(4284..4844)
                     /locus_tag="KLH32_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0140535.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(4970..5320)
                     /locus_tag="KLH32_003899"
     CDS             complement(4970..5320)
                     /locus_tag="KLH32_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBE0140536.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5523..>5868)
                     /gene="intI1"
                     /locus_tag="KLH32_003900"
     CDS             complement(5523..>5868)
                     /gene="intI1"
                     /locus_tag="KLH32_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBE0140537.1"
                     /translation="GHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQ
                     AGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRS
                     PLDALPPLTSER"
BASE COUNT         1162 a         1638 c         1783 g         1285 t
ORIGIN      
        1 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
       61 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      121 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      181 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      241 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      301 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      361 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      421 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      481 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      541 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      601 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      661 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
      721 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
      781 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
      841 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
      901 ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata
      961 aaaacggtta gcgccggggt tggatttttc agcgttccag ctaaggctaa ggcattcctg
     1021 ttcaagcgca agccgggcta taaagcgcat tatcgcttta cccgtgccct tatttcgatc
     1081 gcactgagaa acatacagct ctttaatatg cagctgaccg ctgtatcggg gcgagggata
     1141 aagaatattg caacatgcca ggccagtaat gttattgccg cagcgcgctc tgatcaccag
     1201 ggtaccggaa agccggttga ataacttttt acagagataa tccttcatca acgcttcctg
     1261 aatgatacct tcgccataat aggggtcgtc tcagaaaacg gaaaataaag cacgctaagc
     1321 cggttgcaga ggccgtagcg gcctgaactt ccccgcgccg atcttggcgc tgctgcgcca
     1381 taggtaatca ccggtcaggt tgatgtgctc ccagccgagt ggcgacaggt actgcaatag
     1441 cgagtcatcg acggcatgac cattgccgcg caacgcatgc gccgcacgct ccaggtagac
     1501 cgtgttccac agcacgatgg ccgccgtcac caggttgagg ccgctggccc ggtagcgctg
     1561 ctgctcgaaa ctgcggtcac ggatttcacc aaggcggttg aagaacacgg cacgggccag
     1621 cgcattgcgc gcctcgccct tgttcagccc ggcatgcacg cggcggcgta gctcgacgct
     1681 ttgcagccag tcgaggatga acagcgtgcg ctcgatgcgg cccaactcgc gcagcgcgac
     1741 ggccaagccg ttctggcgcg ggtagctgcc gagtttcctg agcatcagcg aggccgtcac
     1801 cgtgccctgc ttgatcgagg tggccagccg caggatttcg tcccaatggg cgcggacgtg
     1861 cttgatgttg agcgtgccgc cgatcatcgg cttgagcgcg tcataggcgg catcgccctt
     1921 cgggatgtag agcttggtgt cgcccaggtc gcggatgcgc ggcgcgaagc ggaagcccaa
     1981 gaggtgcatc agggcgaaga cgtgatcggt gaagcccgcc gtgtcggtgt agtgctcctc
     2041 gatccgcagg tcggattcgt ggtacagcag gccgtcgagc acgtaggttg agtcgcgcag
     2101 gccgacattg accaccttgg tgtggaatgg cgcgtattgg tcggagatgt gggtgtagaa
     2161 agtccgtcct gggctgctgc catattttgg gttgatgtgc cccgtgctct ttgccttgct
     2221 agcggttcgg aaattctgtc cgtccgatga tgatgtggtg ccatcgcccc agtgcccggc
     2281 aaagggatgc cgaaactgag cgttgaccag ttcagccaac gctgtcgagt acgtttcgtc
     2341 gcgggtatgc caggcttgca gccaagcgag cttcgcgtag gtcgtgccgg ggcaggactc
     2401 ggccatcttg gtcaggccca ggttgatcgc gtcggccagg atcgtggtca acaacaggtt
     2461 cttgtccttg gccagatcgc ccgatttcaa gtgcgtgaag tgccgggtga agcccgtcca
     2521 ctcatcgact tcgagcagca gttcggtgat cttgacgtgc ggcaggacca tggctgtctg
     2581 gtctatcagc gcctgcgcgg tgtcgggcac cgccgcatcc agcggcgtga tcttcaagcc
     2641 cgactcggtg atgatggcat ccggcaggtc gttggctgcc gccatgcggt tgacggtggc
     2701 aagttgtgct tccagcagcg tcagccgctc atgcagatat tgttcgcagt cggtggccac
     2761 ggccagcggc aattcgctgg actgcttgag gctggtgaac ttctcgggcg gtaccaggta
     2821 gtcctcgaag tccttgaact ggcgtgaacc ctgcacccag atgtcgcccg agcgcaggga
     2881 gttcttcaac tcggacagcg cgcacagttc gtagtagcgc cggtcgatgc cggcgtcggt
     2941 catcaccagt ttctgccagc gcggcttgat gaagccggtc ggtgcatcgg ctggcagctt
     3001 gcgggcgttg tcggtgttca tgccgcgcag cacctcaatg gcatcaagca cgtttttggc
     3061 ggcgggcgcg gcccgcagct tgagcacggc aaggaattcc ggtgcatagc ggcgcagggt
     3121 ggcgtagctc tcgccgatgc gatgcaggaa atcgaagtca tcgggttgcg cgagcttctg
     3181 cgcctcggtg acgctctcgg caaaggaatc ccaggacatg acggcctcga tggcggcaaa
     3241 cgcatcgcgg cctgattgct tggcgtcgat cagcgcctga ccgatgcgcc cgtacagacg
     3301 taccttggcg ttgatggcct tgcctgacgc ctggaactgc tgctgatgct tattcttggc
     3361 agcgttaaac agcttaccca ggatgcggtc gtgcaggtcg atgatttcgt cggtgacggt
     3421 ggccatgccc tcggtggcca gcgccacgag agtggcgtag cgccgttgcg gctcgaattt
     3481 ggccaggtcg gcgggtgtca tctggccgcc ctcgcgggca atcttgagca ggcggttctg
     3541 gtgaaccagc cgctcgatgc cggtaggcag atcgagtgcc tgccatgcct tgaggcgttc
     3601 gatgtgttcc agcatatgcc gcgaatttgg cttggccgga gactggcgca accaagccaa
     3661 ccaggtcgtc ttgccgttgt cccggcgctt gagcagatcg tcgaggcggc ggcgatgcgc
     3721 gtccgccagt ggttcggcca aggcgtcgta gatgcgccgg ttagcacggg tgatcgcctc
     3781 ggcactcgcc cgctcgacgg cgttgagggc gggcagaatg accgactgcc gccgcaggtg
     3841 cccgatcaag gcgctggcca gcacgatgcc tttgtcggtt tgcatcgcca gctcggtcag
     3901 catctggacg gcctgccggt aatggctcat ggtgaagggc cggaaaccga acacggtttg
     3961 cagctcgctc aggtgctcgc gccgggtctg ctcccgctgg ccgtactcgt tccagctttc
     4021 gacgccgacc ttgagctggt cggcgaccag cttcaacaag ggcgggaacg gtagttcatc
     4081 gacgcccagg atgacgccgg gaaagcgcag gtaacagagc tgcaccgcga agcccagccg
     4141 attggctggc ccgcgccgct gtcggatgat cgagaggtcg gtatcgttga atgtgtaatg
     4201 tcggatcagg tcgtccttgg agtccggcaa cgccagcagg ctttcccgct cggcggcgga
     4261 caggatggaa cgacgtggca tatttactga tccgttctca agtattgata cagggtttcg
     4321 cgactgattc cgaattcacg agcaagcttg gtcttttgct cgccagcctc gacacgttgg
     4381 cgcagttcgg caatacgctc agacgacagg gatttcttcc tgccacggta agccccgcgt
     4441 tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga tcagggcgcg ctcgaactcg
     4501 gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg gagagtcttc gccagtaaaa
     4561 ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt gtgtcagcgt ttgcacgatc
     4621 cggcgcaaat catcgagatt gcgcgccagg cgatccatgc tatgcaccac cacggtgtcg
     4681 ccggtgcggg cgaagcttat cagcgcttcc agttgcggac gcttgacatc cttgccggat
     4741 gccttgtcgc taaaagcgcg atcaaccttg acgccttcca gttgccgttc cgggttctgg
     4801 tcgaaggtgc tgaccctgat atacccaatg cgctgtccag tcatggaatt ccctgcaaaa
     4861 tgtcagggaa gactctatga ccttcaacga gatatgtcaa taaattcaaa attcaatcct
     4921 atcctgacgc aatttacaca tggcatctga catcaggtta gggtatgcct caacctgacg
     4981 gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct
     5041 ggaagcgctg atagcactcc agcccgcaga aatgctccac gtactcggcc ccttccggcg
     5101 tgaaggcggc atcgagcggg atttccttgc agcacacgca gcaactggtg ctcggttcat
     5161 tggcgttcat ggtggtgttc ctccattggt tgacgaagcc gacgaaggcc gccgccggca
     5221 tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa
     5281 ggcagtcggg tgtttccaca ccctcggcta ccacctccat tgtcgttttc agaagacggc
     5341 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat ccatcaggca
     5401 acgacgggct gctgccggcc atcagcggac gcagggagga ctttccgcaa ccggccgttc
     5461 gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc gccaggattg acttgcgctg
     5521 ccctacctct cactagtgag gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg
     5581 ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga
     5641 tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg
     5701 agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg
     5761 cgctgaaagg tctggtcata catgtgatgg cgacgcacga caccgctccg tggatcggtc
     5821 gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg aatgcccg
//
LOCUS       DAEEDS010000163         5784 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEEDS010000163 DAEEDS010000000
VERSION     DAEEDS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.166"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="KLH32_003901"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="KLH32_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBE0140538.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="KLH32_003902"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="KLH32_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBE0140539.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="KLH32_003903"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="KLH32_003903"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBE0140540.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="KLH32_003904"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="KLH32_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="KLH32_003905"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="KLH32_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBE0140541.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="KLH32_003906"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="KLH32_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBE0140542.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1305 c         1196 g         1640 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttat
//
LOCUS       DAEEDS010000164         5759 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEEDS010000164 DAEEDS010000000
VERSION     DAEEDS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.167"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..403)
                     /locus_tag="KLH32_003907"
     CDS             complement(<1..403)
                     /locus_tag="KLH32_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HBE0140543.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(548..1051)
                     /gene="cheW"
                     /locus_tag="KLH32_003908"
     CDS             complement(548..1051)
                     /gene="cheW"
                     /locus_tag="KLH32_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBE0140544.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1072..3036)
                     /gene="cheA"
                     /locus_tag="KLH32_003909"
     CDS             complement(1072..3036)
                     /gene="cheA"
                     /locus_tag="KLH32_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBE0140545.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3041..3967)
                     /gene="motB"
                     /locus_tag="KLH32_003910"
     CDS             complement(3041..3967)
                     /gene="motB"
                     /locus_tag="KLH32_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBE0140546.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3964..4851)
                     /gene="motA"
                     /locus_tag="KLH32_003911"
     CDS             complement(3964..4851)
                     /gene="motA"
                     /locus_tag="KLH32_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBE0140547.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4978..5556)
                     /gene="flhC"
                     /locus_tag="KLH32_003912"
     CDS             complement(4978..5556)
                     /gene="flhC"
                     /locus_tag="KLH32_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBE0140548.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5559..>5759)
                     /locus_tag="KLH32_003913"
     CDS             complement(5559..>5759)
                     /locus_tag="KLH32_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBE0140549.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1341 a         1553 c         1407 g         1458 t
ORIGIN      
        1 ttaacgctgt gtaatagttt ttatattttt catcaaaatt acggctggta gcgaccattt
       61 caggtaacgg tgccatgctt ttgaattttt tataatgcgt cgcggcctgg gccaatgttt
      121 tcctagcgct atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca
      181 tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg
      241 acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat
      301 ggtgaaggga agaaaaaaac aaactgccgg aaataagctg taacagtgcg aataccccca
      361 gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa
      421 acaagttgat ctcgttatcg gcaagaaggg gggaaacttt attgctgatg ccacccgccg
      481 cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc
      541 gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca
      601 gcagtttttc gatgttcacc agaatcaaca tacggtcgcc cagtgcgccc agtccagtga
      661 gatattctgt tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc tccgccgtca
      721 atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca
      781 ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta
      841 agtcaacaat cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg
      901 tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa
      961 taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg
     1021 ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc
     1081 ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag
     1141 tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt
     1201 actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc
     1261 gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc
     1321 gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc
     1381 cagcacccgc tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc
     1441 cataacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc
     1501 gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga
     1561 ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac
     1621 gccgcgcccg gagacgtcgg tgacctgctc tgccgtggag aagcctggtg caaatatcag
     1681 cattgcaact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt
     1741 tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt
     1801 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
     1861 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
     1921 gtcgataatg cgttctatca ggctcttgtc gagctcggtg gaactgccca ccagcgtcag
     1981 ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa
     2041 gacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
     2101 acgttgtaac tgccccatgc tggtaatcaa atcaccatga ttaaccgggt ccagttcgct
     2161 ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg
     2221 atcaaccttt tctaccgcta cacggatgct ggtggattca ctgctgcgcg tcgttttttc
     2281 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcacag gtggggtagc
     2341 tatttttggc gaaacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
     2401 gagtaccgct gtgatgtcat cttcggcgat atcgcccggt aatattgccg agagagaatc
     2461 cgcccctttc accacgtcat ttaacgttgt cagatgcccc agctcttctt ccagcaggtc
     2521 gacttcgccg gccttcaggc gcgaaaggat aattcgtcgc ggcaactgac tgcgactttg
     2581 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc
     2641 gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcgt agctggcggc
     2701 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
     2761 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag
     2821 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc
     2881 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
     2941 cggctgcaaa accagcagat gctgctccat atcagccaac agttcgtccg cttcatcaaa
     3001 aaatgtctga taaaaatcgc ttatatcgat gctcacgctg tcacctcggt tcggctgatg
     3061 gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttctagggcg cttactggct
     3121 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
     3181 acaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
     3241 ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc
     3301 ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctgtaacct ttctcaccgc
     3361 tggcgtaggg aaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca
     3421 gcacaggcgc gatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg
     3481 ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
     3541 gatcgatttt gagatggaga cgtaacgccc gcagtttcgg atcggactct atcaactggt
     3601 cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga
     3661 tgttcggctg cttattcact tccccctggc tctgggtgta atcatcacca ccgccgggaa
     3721 ttgggctttc actattggaa atgcgatcgc cgcccgtaac cgctgtcgcc agtggagtcc
     3781 ggaagtactc cgcaatctga atcaactctt ttgggctgga gatggagatc agccacatca
     3841 ccaaaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat
     3901 gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
     3961 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
     4021 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
     4081 ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat
     4141 cattttgctg gtttcggcgc ttttctgacg taaaacactc gctagtgggg aaataaatcc
     4201 gtaagccaat aaaataccga ggaaagtccc caccatcgca tgtgcgataa gcacccccag
     4261 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg ccccccatta cagccgcgac
     4321 aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc
     4381 ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat
     4441 gtgaccgctg ataatcaggc gcagataatc gacgataaaa tcaagcatga cgctatccgc
     4501 gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
     4561 cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag
     4621 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgctttcag
     4681 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
     4741 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
     4801 accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc
     4861 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggaa
     4921 aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgccacacc gtatcagtta
     4981 aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag gattctggga
     5041 aagtttacgt ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc
     5101 aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg
     5161 cagtaatcca ctttcaacaa accgcaccaa tgtcctggca cgggttaatg ccagcagtgg
     5221 tccttcttct gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc
     5281 gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc
     5341 atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg
     5401 cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt
     5461 ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat
     5521 atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc
     5581 gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga
     5641 atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg
     5701 tcaaaacgga agtgacaaac cagctgattg gtttctgcca gcttaaccat ttgcggaag
//
LOCUS       DAEEDS010000165         5737 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEEDS010000165 DAEEDS010000000
VERSION     DAEEDS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.168"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..241
                     /locus_tag="KLH32_003914"
     CDS             <1..241
                     /locus_tag="KLH32_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140550.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="KLH32_003915"
     CDS             complement(238..1179)
                     /locus_tag="KLH32_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBE0140551.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="KLH32_003916"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="KLH32_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="KLH32_003917"
     CDS             complement(1684..2904)
                     /locus_tag="KLH32_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBE0140552.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="KLH32_003918"
     CDS             complement(2908..3651)
                     /locus_tag="KLH32_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBE0140553.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="KLH32_003919"
     CDS             complement(3542..5008)
                     /locus_tag="KLH32_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBE0140554.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="KLH32_003920"
     CDS             complement(5008..5241)
                     /locus_tag="KLH32_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBE0140555.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="KLH32_003921"
     CDS             complement(5229..5708)
                     /locus_tag="KLH32_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140556.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1227 a         1618 c         1435 g         1457 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacatg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatga
//
LOCUS       DAEEDS010000166         5725 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEEDS010000166 DAEEDS010000000
VERSION     DAEEDS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.169"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            84..290
                     /locus_tag="KLH32_003922"
     CDS             84..290
                     /locus_tag="KLH32_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140557.1"
                     /translation="MKSVTIEAKTFAEMLGITEGELIFAIKKTGTFKNKTIPQPHEPH
                     KSNNRFLYSDVMRFIESLKDKENR"
     gene            complement(301..573)
                     /locus_tag="KLH32_003923"
                     /pseudo
     CDS             complement(301..573)
                     /locus_tag="KLH32_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            702..2105
                     /locus_tag="KLH32_003924"
     CDS             702..2105
                     /locus_tag="KLH32_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBE0140558.1"
                     /translation="MLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAELLRKVGKNEW
                     SRETLNRWINGKCSPKTLTLAEEELLRKMLPEAPAHHPDYAFRFIDLFAGIGGIRKGF
                     ETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPENDAYAYI
                     NEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFLPQHRE
                     RIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFANEE
                     NQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKLLE
                     PYILKAVNADSCKVERI"
     gene            complement(2139..3353)
                     /locus_tag="KLH32_003925"
     CDS             complement(2139..3353)
                     /locus_tag="KLH32_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II site-specific deoxyribonuclease"
                     /protein_id="HBE0140559.1"
                     /translation="MLMSVFHNWLLEIACENYFVYIKRLSANDTGATGGHQVGLYIPS
                     GIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDSEARAIYYNSRHFGKTRNEKRITRW
                     GRGSPLQDPENTGALTLLAFKLDEQGGDCKEVNIWVCASTDEEDVIETAIGEVIPGAL
                     ISGPAGQILGGLSLQQAPVNHKYILPEDWHLRFPSGSEIIQYAASHYVKNSLDPDEQL
                     LDRRRVEYDIFLLVEELHVLDIIRKGFGSVDEFIALANSVSNRRKSRAGKSLELHLEH
                     LFIEHGLRHFATQAITEGNKKPDFLFPSAGAYHDTEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSLAQYREMRESGVRLVVPSSLHKKYPEAVRAELMTLGAF
                     IAELTGLYADIP"
     gene            3409..3474
                     /locus_tag="KLH32_003926"
                     /pseudo
     CDS             3409..3474
                     /locus_tag="KLH32_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3614..4378)
                     /locus_tag="KLH32_003927"
     CDS             complement(3614..4378)
                     /locus_tag="KLH32_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBE0140560.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4521..4787)
                     /gene="mobC"
                     /locus_tag="KLH32_003928"
     CDS             complement(4521..4787)
                     /gene="mobC"
                     /locus_tag="KLH32_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBE0140561.1"
                     /translation="MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLK
                     QEAIEKLAERIDQHLDHVSELFDKTWSRWHDCHFQKTTAPVDWA"
     gene            complement(5008..5481)
                     /locus_tag="KLH32_003929"
     CDS             complement(5008..5481)
                     /locus_tag="KLH32_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBE0140562.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT         1457 a         1467 c         1382 g         1419 t
ORIGIN      
        1 gaagcgtacc gcagcaacac tagaaacgag caccattgtt aaagatgccg gtaaagaaaa
       61 tcgataccat gagagttaat taaatgaagt cagtaactat agaagcaaaa acatttgctg
      121 aaatgttagg aataacagaa ggtgagttaa tctttgccat taagaaaact ggcacattca
      181 aaaacaagac catcccacaa cctcatgagc cacataaatc aaataataga tttttatatt
      241 cagacgtaat gaggtttata gaatcactaa aagacaaaga gaaccggtaa tgactccaac
      301 ttactgatag tgttttatgt tcagataatg cccgatgacc ttgtcatgca gctccaccga
      361 ttttgagaac gacagtgact tccgtcccag ccttgccaga tgttgtctca gattcaggtt
      421 atgtcgctca atgcgctgag tgtaacgctt gctgataacg tgcagctttc ccttcaggcg
      481 gggttcatac agcggccagc catccgtcat ccataccacg acctcaaagg ccgacagcag
      541 gcccagaaga cgctccagcg tggccaacgt gcgttaccta acaataaacc tgtttaaata
      601 tccagataaa aacattcaat ctgggtcaaa tgagtgatac agtttcaccc ataagaccca
      661 atggaggcaa tatgtctgaa tttgaattac tggcgcagga tctgcttgag aaagcagaag
      721 cggaagaaca actgcgacag gaaaatgata aaaagctgct cgggcaggtg ctggaaatct
      781 atgaccagaa gtacgtggct gaactgctta gaaaagttgg taaaaatgag tggagtcgcg
      841 agactcttaa tcgctggatt aatggtaagt gctcacctaa gacgctgacg ttagccgaag
      901 aggaacttct acgaaaaatg cttccggaag cgcctgcaca tcaccctgac tatgccttcc
      961 ggtttattga cctgtttgct gggattggag gtatacggaa gggcttcgaa accatcggtg
     1021 gccagtgcgt ttttaccagt gaatggaata aagaggctgt gcgcacatat aaagctaact
     1081 ggtttaacga tgctcaggaa cacactttca atctcgatat tcgtgaagtc acgctcagtg
     1141 ataaacctga agtacctgaa aacgatgcct atgcttacat taatgagcat gtgccggatc
     1201 atgatgtact tctagcaggt ttcccctgtc aaccgtttag ccttgcgggc gtaagcaaga
     1261 aaaactcgct cgggcgcgcg catggtttcg aatgtgaggc tcagggaacg cttttcttcg
     1321 atgtggcgcg tattatccgc gcaaaaaaac ctgccatctt tgttcttgaa aacgttaaaa
     1381 acctgaagag ccatgacaag ggtaaaacct ttaaagtcat catggatacc ctcgacgaac
     1441 tgggctatga agttgcggat gcagctgaga tgggcaaaaa cgatcctaaa gttatcgacg
     1501 gaaagcactt tttacctcag caccgagaac gtatcgtttt ggtcggtttc cgccgtgatc
     1561 tgaacattca ccagggcttt accctgcgcg atattagtcg tttttatccg gaacagcgtc
     1621 cgtcatttgg cgaactgctg gaacccgtgg ttgacagcaa atatatactg acgccgaaac
     1681 tctgggagta tctctataac tacgccaaaa agcacgcagc taagggtaac ggattcggtt
     1741 ttggcctcgt taatcctgaa aataaagaaa gcattgcccg tacgctttct gctcgctatc
     1801 acaaagacgg gtctgaaatt ctgatagacc gtggctggga tatggccaca ggtgaaacag
     1861 acttcgcgaa cgaagaaaat caggcgcatc ggccccgcag gctgactccg cgagagtgcg
     1921 cgcgccttat gggttttgaa aaagtagatg gcaggccttt tcgcattcct gtgtcagaca
     1981 ctcagtcgta caggcagttc ggtaactccg tagtggtgcc cgtgtttgaa gccgtagcca
     2041 aactgcttga accttatatc ctgaaagcgg ttaatgccga ttcgtgcaag gttgaacgaa
     2101 tctgatcgct cctcccggta tttatgccgg gagataatct atggaatatc tgcgtaaagc
     2161 cctgtcagct cagcaataaa cgcacctagc gtcattagct cagctctcac cgcctccggg
     2221 tattttttgt gcagagatga tggcacgacc aatctgacac ccgactcccg catctcccga
     2281 tattgagcca gagaaactcc ctcctggagt gtaaacagat gcacctgatg aattttatcg
     2341 gcctcattca gtatctgacg ccagcgatcc ttacaggtag tcttgactgc cagcatgcgc
     2401 agattttcta cgggaaactc agtatcgtgg taagcccctg cggaagggaa aaggaaatcg
     2461 ggttttttat taccttctgt gatggcctgc gtcgcaaagt gtcgcaggcc gtgctcaatg
     2521 aatagatgct ccaggtgcag ttccagcgac ttcccggctc tggatttacg gcgattgctg
     2581 acagaattgg ccagcgcaat aaattcatcc acagagccaa atcctttccg gatgatatcc
     2641 agaacatgca gttcctcaac caatagaaat atgtcgtact ccacgcgccg gcggtcaaga
     2701 agttgctcat ccggatcaag ggaatttttc acataatggc tggctgcata ctgaataatt
     2761 tcacttcccg acggaaagcg caggtgccag tcttcaggta gaatatattt atgatttact
     2821 ggcgcttgct gtagagatag tccgcctaga atctgtcctg cggggccgga tataagcgct
     2881 ccgggtataa cttcaccaat agcggtctca atgacgtcct cttcatcagt gctggcgcat
     2941 acccaaatat ttacttcctt acagtccccc ccttgctcat caagcttgaa agccaggagc
     3001 gtcagagccc ctgtattttc aggatcctga agtgggctgc ctctacccca gcgggtaatc
     3061 cttttttcat tccgggtttt accaaaatga cggctgttat aataaattgc cctggcttcg
     3121 ctgtcagggc aatcatgcga tgacacatgt gcggtgagaa aaaccgaagg gttcagttca
     3181 cgggtatggt tgatagacgg aaagagtttt tcaacgatac ctgaagggat ataaagccct
     3241 acctggtgac cacctgttgc gccggtatcg ttggcggaaa ggcgtttgat gtagacgaag
     3301 taattctcac atgcgatctc aagtagccag ttgtggaaaa ccgacataag catcccctgt
     3361 taccctgaaa ctctactcac cattttttca tgattatata caaacagtgt cattttcaga
     3421 agacgactgc accagttgat tgggcgtaat ggctgttgtg cagccagctc ctgacagttc
     3481 aatatcagaa gtgatctgca ccaatctcga ctatgctcaa tactcgtgtg caccaaagcg
     3541 aggtgagcat ggcgacggag gctctgttgc aaagattggc ggcagtcaga ggtaggctgt
     3601 cgctctgcgc cgatcaggcg gctgctgcga aatggtggtt gagcatgccc atggcctccg
     3661 tcagcgccga gggcccaatg ccaaaagctc tctccacaag gcgcacctcg cccctgatgc
     3721 cgggctgcag gcaccagggg cgagcctgtc ctttgcgcag ggctcgcatg acttcgaatc
     3781 ccttgatcgt ggcataggcc gtggggatcg atttgaaacc gcgcaccggc ttgatcagta
     3841 tcttgagctt tccgtgatcg gcctcgatca cgttattgag atacttcacc tgccggtggg
     3901 ccgtctcccg gtccagcttt ccttcgcgct tcaattcggt gatcgctgca ccatagctcg
     3961 gcgctttgtc ggtattgagc gtggcaggct tttcccagtg cttcaggcct cgcagggcct
     4021 tgcccaggaa ccgcttcgct gccttggcgc tgcgggtcgg cgacaggtag aaatcgatcg
     4081 tgtcgccccg cttgtcgact gcccggtaca ggtaggtcca cttgccccgc accttgacgt
     4141 aggtttcatc caggcgccag ctcggatcaa agccacgccg ccagaaccag cgcagccgct
     4201 tctccatctc cggggcgtag cactggaccc agcgatagat cgtcgtatgg tcgaccgaaa
     4261 tgccgcgttc cgccagcatt tcctcaaggt cgcgatagct gatcggatag cgacaatacc
     4321 agcgcaccgc ccacaggatc acatcaccct ggaaatggcg ccacttgaaa tccgtcatcg
     4381 ttccgtccgt ccaatctccg ccaagcatgc tcaagcttca cgatttttgc aacagagccc
     4441 acacgagtat tgagcatagt cgagattggt gcagatcact tctgatattg aactgtcagg
     4501 agctggctgc acaacagcca ttacgcccaa tcaactggtg cagtcgtctt ctgaaaatga
     4561 caatcgtgcc accggctcca ggtcttatcg aacagctcag acacatggtc caaatgctgg
     4621 tcgatccgtt cggccagttt ttcgatggcc tcttgcttga gcttgtcact ttccctgaac
     4681 tcgatgttca aggccctggc cacctggttg aggttccggc cgatagctcc aagctgccgg
     4741 cacgattccc gtacggtgtt cacttcttca tcagtgagga ccggcatccg gttcaacgac
     4801 cgcagtcatt tctgtaccta aacagtcctg cgaatatgtg agttttgacc taatgagcca
     4861 caaaaactca ttttcgccca cgataatttt tctacttcct ttctttggaa tgctcatttc
     4921 aatattcaca taacgtcgct atcaatcgcg gaaaaggcgt agtgcgcgaa gcgaacgaag
     4981 ctttttgccg tcgattgcat agctttgtta accctttttc caaatttgat agcaatagtt
     5041 aatgtttgaa gtaaagtgtt gctcaaaaac aacttcgaag gtatttggaa tactcgggaa
     5101 gaaaacatcc ccctctggct cgatgtcgat cgtcgataag tggagcgtag aggccatggg
     5161 taatgtttct cggtaaattt ctccgccacc agacactata acgtgaccgg tgaattcagc
     5221 tagcctgtcc atggcctctt cgattgactg aaatacaact acattgtcat catttgatgt
     5281 ccaacctgag cgggtaacga ccgcgtattt cctattgggg agtgcgccca tagattcaaa
     5341 cgtcttgcga cccaccagaa gccactgatt gtaggtcaat gctttaaaaa gtagctgctc
     5401 ccctttcgcg gaccagggta tgtctggacc gcaaccaatc acgccgtttt tcgctttcgc
     5461 agccatcaat gatactttca aggttctcat cctgggttaa ctttgtttta gggcgactgc
     5521 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     5581 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     5641 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     5701 agcgcatacg ctacttgcat tacag
//
LOCUS       DAEEDS010000167         5703 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEEDS010000167 DAEEDS010000000
VERSION     DAEEDS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.170"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..220)
                     /locus_tag="KLH32_003930"
     CDS             complement(<1..220)
                     /locus_tag="KLH32_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBE0140563.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(217..1125)
                     /locus_tag="KLH32_003931"
     CDS             complement(217..1125)
                     /locus_tag="KLH32_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE0140564.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1321..2088
                     /locus_tag="KLH32_003932"
                     /pseudo
     CDS             1321..2088
                     /locus_tag="KLH32_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2139..2791)
                     /gene="pphB"
                     /locus_tag="KLH32_003933"
                     /pseudo
     CDS             complement(2139..2791)
                     /gene="pphB"
                     /locus_tag="KLH32_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2897..5458)
                     /gene="mutS"
                     /locus_tag="KLH32_003934"
     CDS             complement(2897..5458)
                     /gene="mutS"
                     /locus_tag="KLH32_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBE0140565.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5534..>5703
                     /locus_tag="KLH32_003935"
     CDS             5534..>5703
                     /locus_tag="KLH32_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140566.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1471 g         1413 t
ORIGIN      
        1 gctgttgcaa ccagctcaga gccgccagcg attgctgtgt ggcttcaacc accggacagg
       61 agcgcgtttt caggctgatc accagtgcgt cgattgcttc cggcatttta cctgttggaa
      121 caccgttaat ttgtaccgtt ggtagaccgt tttccaccag aaaactggcg atatccgtcg
      181 cgccggtaaa atcatcggcg ataacgccaa tcttgatcat gatttcgctc ccggtagagt
      241 gatgccagag aaaatcttga taactgcgct atcgtcttct ttcccgtaac ccgcgttact
      301 ggcgctggtg aacatattca atgctgttga ggccaatggc agcgggaagt gcagggcttt
      361 ggctgtatcg gcaaccagac caagatcctt aacaaaaata tcgacggctg aatgcggggt
      421 gtaatcgcca tccaccacat gacgcatccg gttttcgaac atccaggaat ttccggcggc
      481 attggtcacg acgtcataca tcacatccag cgggatcccc gcacgggctg caagtgccat
      541 cgcttcggct ccggcagcaa tatgtacgcc cgctaacaac tggtgaataa tttttacggt
      601 cgaacctagt cccggttctg cacctatgcg ataaactttt ccggcaacgg cttccagcac
      661 gggtgccagt cgttcaaagg caatatcgct accggaggcc atgacagtca tttcaccgtt
      721 agcggctttt actgcaccac cagaaactgg cgcatccagc atttccagat cgaatccagc
      781 cagagcggta gcaatttctt gcgcatcagc actagcgata gtggaagaaa ccattactgc
      841 cgtaccgggt ttcagatgtt gtgcaacgcc tgtttcacca aacagcacct gtttaacctg
      901 ggccgcattg accaccagca ccagcagagc gtccagtttt tcggcaaacg tcgcggcgtt
      961 atcagaaacc ccgcaagcac ctgcctcttt caacgtagcg caggcattgc tgttcaggtc
     1021 tgcgccccag gtagaaaaaa ctgcgcggac acatgacagt gctgctccca ttcccattga
     1081 ccctaagcca acgataccga catgaaactc agatcccgtt ttcatctgct ctccttgtta
     1141 atttaagtga tattttgttt gatattgtga acataagcgc tgggagataa cgatatggtg
     1201 agatgattca cataaattaa cattttgtgt tattttatgt gaactaagcg ttagttgcgc
     1261 cgcgcacgtt tcgcaggcaa atagcgtaga atgtcagcag gacaaaggaa ggagcaaaag
     1321 ttgatacccg tagagcgtcg ccaaatcatc cttgagatgg tagctgaaaa aggcattgtc
     1381 agtattgctg aactaacgga cagaattaat gtgtcacata tgaccattcg tcgggattta
     1441 caaaaactgg agcagcaggg agccgttgtg ctggtgtccg gaggcgtcta gtctccggga
     1501 cgcgtggcgc atgaaccttc tcatcaggta aaaactgcgc tggcaatgac gcaaaaagcg
     1561 gctattggca agctggcggc aagtcttgtt ctgccgggaa gttgtatcta tctggatgcg
     1621 ggaacgacca cgttagcgat agcacagcat ctgattcaca tggagtcact gactgtggtc
     1681 acaaacgatt tcgttattgc gaactacttg ctcgacaaca gtaattgcac aattattcac
     1741 actggcggtg cagtgtgtcg ggaaaaccgt tcctgtgtcg gggaagccgc tgcgaccatg
     1801 ctgtgcagcc tgatgattga tcaggctttt atttctgcat cgtcatggag tgtgcggggg
     1861 atttctacgc cagcggaaga taaagtcacg gtgaaacggg cgattgccag tgccagccgc
     1921 cagcgagttt tggtctgtga tgcgacgaaa tatggtcagg tggcgacatg gctggcgtta
     1981 ccattaagcg agtttgatca gattattaca gacgacggtt tgccggagag tgccagtcgc
     2041 gcgctggcga agcaggatct ctctttgctg gtagcgaaaa atgaataatg gcctgcaata
     2101 acatctggtt actcatgctt cacagaagaa gcatgagact accttatttt ataaaatgac
     2161 agccgcccac ttttcggcga tccggtatca atataaatct ggttagcgaa cgtctgaatg
     2221 ttatcaaaca tcatatgtcc aaatataaaa taatcagcgc cgtttatttg ttgtaactcg
     2281 ccattaagcg atttctgcac acgataaaca ggccagagta attcgctctc cgctatttct
     2341 ttgccaaaga gatattcatc acccggataa tctgcatgtg cgatgacata ttttattatg
     2401 tcgttagtga tttcaataat atgtggaagg tgatggaatt tcagcaacag atctattgcc
     2461 tcttgttgca atcatttaaa tcgaaaaacc agtcaccacc gctggcaagc cacatattgc
     2521 catcgccggt cgcgaacgca tccagcgcca ttgcttcgtg gttgccttta acggagataa
     2581 accagggctg gtttagcagg cgcaggacgt caagactctc cggcccacga tcaatattat
     2641 cgccgacaga aataagtaag tcggtttcgg ggaaaaaaga gagttgatgt aagcgggatt
     2701 gtaataactg atattcacca tgaatatcac caacgaccca tatatggcga tagtgatggg
     2761 cattgatttt ttggtagcgt gtagatggca tggttttacc ctgtaaaata agctttccta
     2821 ttatacaggg tatttttatt tgattcgtca gttgtcgtta atattcccga tagcaaaaga
     2881 ctatcgggaa ttgttattac accagactct tcaagcgata aatccactcc agcgcctgac
     2941 gcggagtcag tgaatccggg tcgagatttt ccagtgcttc gaccgcaggc gaagtttctt
     3001 ctggcactga cagcaaagac atctgcgtac catccacttg cgtcgcggcg gcgttcggcg
     3061 aaatgctttc cagctcacgc agtttttgcc gtgcgcgctt aataacctct ttcggtacgc
     3121 ccgccagagc agcaaccgcc agaccgtagc ttttgctcgc cgcgccgtcc tgcacgctgt
     3181 gcataaaggc gatagtgtcg ccgtgctcca gcgcatcgag atgtacgttg gcgacgcctt
     3241 ccattttctc cggtagctgg gtcagctcga aatagtgggt ggcaaacagc gtcaatgcct
     3301 taatcttatt cgccagattt tctgcgcacg cccacgccag cgacagacca tcgtaggtgg
     3361 acgttccacg cccgatctca tccattaaca ccagactgta ttcggtggcg ttatgtaaaa
     3421 tattggcggt ttcagtcatc tccaccataa aggttgagcg cccggaagcc aggtcatctg
     3481 ccgcgcctac gcgggtaaag atgcgatcga taggtccaat ctcgactttt tgtgccggta
     3541 catagctgcc gatgtaggcc atcagcgcaa tcagtgcggt ctggcgcata taggtacttt
     3601 taccgcccat gttcgggccg gtaataatca acatacggcg ctgcggcgac agattcagcg
     3661 ggttagcgat aaatggctca ttcagtactt gttcaactac cggatggcga ccttcggtaa
     3721 tgcgaatgcc cggtttatca atgaaggtcg ggcaggtgta gttcagggta taggcccgtt
     3781 ccgccaggtt aaccagcacg tcgagttccg ccagcgcgct cgcgctctgt tgcaacgctt
     3841 ccagatgcgg caacagcagg tcgaacagct cttcataaag ctgtttttcc agtgccagtg
     3901 ctttgccttt tgaggtgaga actttatctt cgtactcttt tagctctgga atgatgtagc
     3961 gctcggcgtt tttcagcgtc tggcgacgca tgtagttgat gggtgccaga tggctttgcc
     4021 cacggctgat ttgaatgtag tagccgtgca ccgcattaaa gccaactttt agcgtgtcca
     4081 ggccggtacg ttcacgctcg cggacttcca gacgctccag ataatcggtc gcgccgtcag
     4141 ccagcgcgcg ccactcatcc agctcttcgt tatagcccga tgcgataaca ccaccgtcgc
     4201 gtaccagcac cggcggtgtg tcgatgattg ctcgctccag cagatcgcgc agctcggcaa
     4261 actcgcccat cttctcacgt agcgcctgta ccggtgcact atcgacagtt tctaactgcg
     4321 cacgcagctc cggcagttgc tggaaagcgt ggcgcatacg ggccagatcg cgtgggcgag
     4381 cagttcgtaa agccagacgt gccagaatac gttccaggtc gccgacctga cgcagtaccg
     4441 gctgcaactc ggcggtgaaa tcctgcaatg cgccaatagt ttgctggcgc tcaagcaaca
     4501 cgcgggtatc gcgcactggc atatgcagcc agcgtttcag catacgacta cccatcggcg
     4561 tgacggtgca gtcgagcaca gaagccagcg tattttccgc accgcccgcc aggttctgag
     4621 taatttccag gttacgacgt gtcgcggcat ccataatgat gctgtcctgc tcacgttcca
     4681 tggttataga acgaatatgc ggcagggtcg tgcgttgggt atctttcgca tactgcaaca
     4741 gacaaccggc agcacaaagt ccgcgcggcg cgttctcgac gccaaaaccg accagatcgc
     4801 gggtgccaaa ttgcagattc aactgctggc gcgcggtgtc gatttcaaac tcccacagcg
     4861 ggcgacggcg caggccgcga cggccttcaa tcagcgacat ctcggcgaaa tcttctgcat
     4921 acagcagttc cgccggatta gagcgttgca gctctgccgc catcgtttcg cggtcggccg
     4981 gttcgctcag gcgaaaacgc ccggagctga tatccagcgt cgcgtagccg aaacctttgc
     5041 tgtcctgcca gatagccgcc agcaggttgt cctgacgctc ctgtaacagg gcttcatcgc
     5101 tgatggtgcc tggcgtaacg atacgcacaa ctttgcgctc aaccggacct ttgctggtcg
     5161 ccggatcgcc aatttgttcg cagatggcaa cggactctcc ctgattcacc agtttggcga
     5221 gatagttttc caccgcatgg tagggaatcc ccgccatcgg gatcggctct cccgccgaag
     5281 caccgcgttt ggtcagtgaa atatccagca gttgcgacgc gcgttttgcg tcgtcataaa
     5341 acagttcata aaaatcaccc atccggtaaa acagcaggat ctcgggatgc tgggctttca
     5401 gcttgagata ctgctgcatc atgggcgtat gggcgtcgaa attttctatt gcactcatgg
     5461 ggttatgtcc ggttccctga tattaaatgg ggtgtgatgg tttttatttt cgttgtaatc
     5521 acataaggcg cacatgatac cggagttaat catttcaggg gaagaagcga acggcgagtt
     5581 cttgatgctg gttccgggaa agatgctgtt ttgcagcttt tgcgtgatgc ttatgaagca
     5641 agtacgcaaa attgtatcga ttggcgaacc cttcttcttt agagtcatag tgccctttaa
     5701 gat
//
LOCUS       DAEEDS010000168         5692 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEEDS010000168 DAEEDS010000000
VERSION     DAEEDS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.171"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(25..591)
                     /gene="satP"
                     /locus_tag="KLH32_003936"
     CDS             complement(25..591)
                     /gene="satP"
                     /locus_tag="KLH32_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBE0140567.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(740..1453)
                     /gene="msyB"
                     /locus_tag="KLH32_003937"
     CDS             complement(740..1453)
                     /gene="msyB"
                     /locus_tag="KLH32_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBE0140568.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1479..1883)
                     /locus_tag="KLH32_003938"
     CDS             complement(1479..1883)
                     /locus_tag="KLH32_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBE0140569.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2260..4176
                     /gene="dnaK"
                     /locus_tag="KLH32_003939"
     CDS             2260..4176
                     /gene="dnaK"
                     /locus_tag="KLH32_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBE0140570.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4265..5395
                     /gene="dnaJ"
                     /locus_tag="KLH32_003940"
     CDS             4265..5395
                     /gene="dnaJ"
                     /locus_tag="KLH32_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBE0140571.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5499..5651)
                     /gene="mokC"
                     /locus_tag="KLH32_003941"
     CDS             complement(5499..5651)
                     /gene="mokC"
                     /locus_tag="KLH32_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBE0140572.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1412 c         1495 g         1289 t
ORIGIN      
        1 ccgagcgggg ggatctcaag acaattagtg ggattcacca atcggcagaa cggtgcgacc
       61 aaactgctcg ttcagcactt cacccatcgc cagatagatt gcgctggcac cgcagatcag
      121 accaatccag ccggcaaagt ggatgatcgc ggcattaccg gcaatgttac cgatcgccag
      181 cagggcaaac agcacggtca ggctaaagaa aacgaattgc aggacgcgcg cgcctttcag
      241 agtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga caccaaggaa
      301 ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg caaccagcgt
      361 cagccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta
      421 ctccagcaga ccagcaaaaa tttgcgcgat gccgccgtag aaaatgccca tggcaagaat
      481 aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc
      541 gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca taattcctca
      601 aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taactgtagt gttttcaggg
      661 cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa
      721 ttttccccgg tttccggtat cagacctgag tggcgctaac catccggcgc aggcaggcga
      781 tttgcagtac ggctggaatc gtcacgcgat aggtgctgcc gctgaccgct ttaaccccat
      841 ttaatgctgc gcccacaggg cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta
      901 cgctcattgc tgcgtgggtg cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca
      961 caccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc agctcattca
     1021 ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccatt ttcttccagg
     1081 tattacgcag aaattgttcc agtaactgtt gctcaatttc aaacgtagac atctctttgt
     1141 cggctttcag cttcaatcgc tttgaaacat cgagcaaaat ggcccgatac aatttaccgt
     1201 gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct ccggcaatca
     1261 gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac agttcgttgc
     1321 gcatcagtat gctggagagg cgagttttgc ctttttcatt atgggtgagc aatcgggcga
     1381 aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa tcattcaggt
     1441 aattaacatt cattttttgt ggcttctata ttctggcgtt agtcgtcgcc gataattttc
     1501 agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt tgagacgcag
     1561 cgtttattgt cgttatcgct gttaatgttg atccagtcag tggtttgccc ttcttttatt
     1621 tctgaaggaa tattcaggct ctgactggcg ctacgggcgg ctttgaaata aaccgatgca
     1681 ccacttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac aatgcgacaa
     1741 acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat gacgccgagg
     1801 agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc caggctggcg
     1861 gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag ggggatgaat
     1921 acccactagt ttactgctga taaagagaag attcaggcac gtaatctttt ctttttatta
     1981 caattttttg atgaacgcct tggctgcgat tcattcttta tatgaataaa attgctgtca
     2041 attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg
     2101 catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc agtgagtgag
     2161 tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat tacagactca
     2221 caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat aattggtatc
     2281 gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccattcc tcgcgtgctg
     2341 gagaacgccg aaggcgatcg caccacgcct tctatcattg cctataccca ggatggtgaa
     2401 actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa caccctgttt
     2461 gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg tgatgtttcc
     2521 atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga agttaaaggc
     2581 cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct
     2641 gaagattacc tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc atactttaac
     2701 gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga agtaaaacgt
     2761 atcatcaacg aaccgaccgc agctgcgttg gcttacggtc tggacaaagg cactggcaac
     2821 cgtactatcg cggtttatga cctggggggt ggtactttcg atatttctat tatcgaaatc
     2881 gacgaagttg acggcgaaaa aaccttcgaa gttctggcaa ccaacggtga tacccacctg
     2941 ggtggtgaag acttcgacag ccgtctgatc aactatctgg ttgaagaatt caagaaagat
     3001 cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa
     3061 aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc atacatcact
     3121 gcagacgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa actggaaagc
     3181 ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact gcaggatgct
     3241 ggcctgtccg tatctgatat cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca
     3301 atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg
     3361 gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg cgacgtaaaa
     3421 gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat gggcggtgtg
     3481 atgacgacgc tgatcgcgaa aaacaccact atcccgacca agcacagcca ggtgttctct
     3541 accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt
     3601 gcggctgata acaaatctct gggtcagttc aacctagatg gtatcaaccc ggcaccgcgc
     3661 ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatcct gcacgtttcc
     3721 gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc ttctggtctg
     3781 aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga agctgaccgt
     3841 aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca cagcacccgt
     3901 aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc tatcgagtct
     3961 gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa
     4021 atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc
     4081 cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc
     4141 gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg ggtaattata
     4201 ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa
     4261 aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag cggaagagcg
     4321 tgaaatcaga aaggcctaca aacgcctggc catgaaatac cacccggacc gtaaccaggg
     4381 tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag ttctgaccga
     4441 ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc aaggaggcat
     4501 gggcggcggt ggttttggcg gcggcgcaga cttcagcgat atttttggtg acgttttcgg
     4561 cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta
     4621 taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga tccgcattcc
     4681 gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta cacagccgca
     4741 gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat tcttcgctgt
     4801 acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa
     4861 atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg
     4921 ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc
     4981 accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt tcgagcgtga
     5041 aggcaacaac ctgtattgcg aagtcccgat caacttcgct atggcggcgc tgggtggcga
     5101 aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg aaacccagac
     5161 cggtaagcta ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg
     5221 tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaaa agcagaaaca
     5281 gctgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg
     5341 ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc gctaacctcc
     5401 ccaaaagcct gcccgtgggc aggcctgggt aaaaataggg tgcgttgaag atatgcgagc
     5461 acctgtaaag tggcggggat cactccccgc cgttgctctt actcggattc gtaagccgtg
     5521 aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc ttttctcgtt
     5581 accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat catcgcctta
     5641 tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac tc
//
LOCUS       DAEEDS010000169         5683 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEEDS010000169 DAEEDS010000000
VERSION     DAEEDS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.172"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="KLH32_003942"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="KLH32_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBE0140573.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="KLH32_003943"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="KLH32_003943"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBE0140574.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="KLH32_003944"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="KLH32_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBE0140575.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KLH32_003945"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KLH32_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBE0140576.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1488 c         1349 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtc
//
LOCUS       DAEEDS010000170         5642 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEEDS010000170 DAEEDS010000000
VERSION     DAEEDS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.173"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            263..1189
                     /gene="tauA"
                     /locus_tag="KLH32_003946"
     CDS             263..1189
                     /gene="tauA"
                     /locus_tag="KLH32_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0140577.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1202..1969
                     /gene="tauB"
                     /locus_tag="KLH32_003947"
     CDS             1202..1969
                     /gene="tauB"
                     /locus_tag="KLH32_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBE0140578.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1966..2793
                     /gene="tauC"
                     /locus_tag="KLH32_003948"
     CDS             1966..2793
                     /gene="tauC"
                     /locus_tag="KLH32_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBE0140579.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2790..3641
                     /gene="tauD"
                     /locus_tag="KLH32_003949"
                     /pseudo
     CDS             2790..3641
                     /gene="tauD"
                     /locus_tag="KLH32_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KLH32_003950"
     CDS             complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KLH32_003950"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBE0140580.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5245..5613
                     /locus_tag="KLH32_003951"
                     /pseudo
     CDS             5245..5613
                     /locus_tag="KLH32_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1325 a         1409 c         1529 g         1379 t
ORIGIN      
        1 attgacctgc ctttagggtg cctttaaaat tcgtttttaa aggctacgat gacatttttg
       61 aagagtctta aaagaattaa aatacacggc gcgcctatct tgaatgtttt atcgatatcg
      121 aagcattgag catttttaac tcgttttatc ttgaatgcaa tcggcgcata acttattgaa
      181 tcccatcaaa tcccgtcttt ttttacccat tcccggttat cttgaataaa ttacctagtc
      241 atataacaca cttcttgccg cactggcatt catcgctttt caggcacagg cggtgaacgt
      301 caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg gctcaggccg acaacacctt
      361 tgctaaagag agcggggcga gcgtggactg gcgtaagttt gacagcggag ccagcatcgt
      421 gcgggcgctg gcttctggcg acgtgcaaat cggcaacctc ggttccagcc cattagcggt
      481 tgcagccagc caacaggtgc cgattgaagt cttcttgctg gcgtcaaaac tgggtaactc
      541 cgaagcgctg gtggtaaaga aaactatcag caaacccgaa gatttgattg gtaaacgcat
      601 tgccgtaccg tttatctcca ccacccacta cagcctgctg gcggcgctga aacactgggg
      661 gattaaacca gggctagtgg agattgtgaa cctgcagccg cccgcgatta tcgccgcatg
      721 gcagcggggt gatattgatg gtgcttatgt ctgggcaccg gcggttaacg ccctggaaaa
      781 agacggcaag gtgctgaccg attctgaaca ggtcgggcag tggggtgcgc caacgctgga
      841 cgtctgggtg gtgcgcaaag attttgccga gaaacatcct gaggtcgtga aagcgttcgc
      901 caaaagcgcc atcgatgcac agcaaccgta cattgctaac cctgacgcgt ggctgaaaca
      961 gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg cctgaaggtg acgttccggg
     1021 gctggtgaag ggaaatacct atctgacgcc gcagcaacaa acggcagaac tgaccggacc
     1081 ggtgaacaaa gcaatcatcg acaccgcgca gtttttgaaa gagcagggca aggtcccggc
     1141 tgtagcgaat gattacagcc agtacgttac ctcgcgcttc gtgcaataaa aggaggcgca
     1201 gatgctgcaa atctctcatc tctatgccga ttatggcggc aaaccggcac tggaagatat
     1261 caacctgacg ctggaaagcg gcgagctact ggtggtgctg gggccgtccg gctgcggtaa
     1321 aaccaccctg ctgaatctga ttgccggttt tgtgccttat cagcatggca gcattcaact
     1381 ggcgggtaag cgtattgagg gaccgggagc agagcgtggc gtagtttttc agaatgaagg
     1441 gctgctaccg tggcgcaatg tacaggacaa cgtggcgttc ggcctgcaat tggcaggtat
     1501 agagaaaatg cagcgactgg aaatcgcgca ccagatgctg aaaaaagtgg ggctggaagg
     1561 cgcagaaaaa cgctatatct ggcagctttc cgatggtcaa cgtcagcggg tggggattgc
     1621 ccgcgcgctg gcggcgaatc cccagctgtt attactcgac gaaccgtttg gtgcgctgga
     1681 cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa ctctggcagg agacgggcaa
     1741 gcaggtgctg ttgattaccc acgatataga agaagcggtg tttatggcga ctgaactggt
     1801 tctgctttca tccggccctg gccgtgtgct ggagcggctg cggctcaact ttgctcgccg
     1861 ctttgttgcg ggagagtcga gccgcagcat caagtccgat ccacaattca tcgccatgcg
     1921 cgaatatgtt ttaagccgcg tatttgagca acgggaggcg ttctcatgag tgtgctcatt
     1981 aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc cgctctcgcg tcaggtgacc
     2041 ttaagcattg gcacgttagc ggttttactc actgtatggt ggacggtggc ggcgctgcaa
     2101 ctgattagcc cgctgttttt gccgccgccg caacaagtac tggcaaaact gctcaccatt
     2161 gccggaccgc agggatttat ggacgccacg ctgtggcagc atctggcagc cagcctgacg
     2221 cgcattgtgc tggcgctact cgcggcggtg gtgatcggta ttccggtcgg gatcgccatg
     2281 gggctaagcc cgacggtgcg cggcattctg gatccggtaa tcgaacttta tcgtccggtg
     2341 ccaccgctgg cttatttgcc gctgatggtg atctggtttg gtattggtga aacctcgaag
     2401 atcttactga tctatttagc gatttttgcg ccggtggcga tgtcggcgct ggcaggggtg
     2461 aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc tgggtgccag ccgtgcgcag
     2521 gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa tcctcaccgg attacgcatt
     2581 ggtctgggtg tgggctggtc tacgctggta gcggcggagc tgattgccgc gacacgcggt
     2641 ttaggattta tggttcagtc agcgggtgaa tttctcgcga ctgacgtggt actggcgggg
     2701 atcgcggtga ttgcgattat cgcctttctt ttagaactgg gtctgcgcgc gttacagcgc
     2761 cgtctgacgc cctggcatgg agaagtacaa tgagtgaacg tctgagcatt accccgctgg
     2821 ggccgtatat cggcgcacaa atttcgggtg ccgacctgac gcgcccgtta agcgataatc
     2881 agtttgaaca gctttaccat gcggtgctgc gccatcaggt ggtatttctg cgcgatcaag
     2941 ctattacgcc gcagcagcaa cgcgcgctgg cccagcgttt tggcgaactg catattcacc
     3001 ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt gctggatacc cataacgata
     3061 atccgccgga taacgacaac tggcataccg atgtgacatt tattgaaacg ccacccgcag
     3121 gggcgattct ggcagctaaa gagttacctt cgaccggcgg tgatacgctc tggaccagcg
     3181 gtattgcggc ctatgaggcg ctctctgttc ccttccgcca gctgctgagc tggctgcgtg
     3241 cggagcatga tttccgtaaa tcattcccgg aatacaaata ccgcaaaacc gaggaggaac
     3301 atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt gctacatccg gtggtgcgaa
     3361 cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga aggctttact acgtgaattg
     3421 tcgatgtcag cgagaaagag agcgaagcct tgttaggctt tttattcgcc catatcacta
     3481 aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga tattgcgatt tgggataacc
     3541 gcgtgactca gcactatgcc aatgccgatt acctgccaca gcggcggatt atgcatcggg
     3601 cgacgatcct tggggataaa ccgttttatc gggcggggtg atacgagtgc ggacggttcc
     3661 ctcgccccct tggggagagg gttagggtga gggggcgttc actgtacttt caacaggtta
     3721 actccccctt tctgagagga aaaaaattaa cgcagaatct tcttctcagc caaatccagc
     3781 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa ttgaacctaa gctttcgagc
     3841 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcgtactca
     3901 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
     3961 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
     4021 agtgactcac gaatcgcctc gcgacggttc attgggttca tctgatagct tttgcggtcg
     4081 ccttttaatg cgcttcctgc agcttcacgg aacgggccat aaaaggaaga ggcgaacttg
     4141 gtcgaatacg acataatcgc cgtatcttta aagcctgcag cgtccagcgc ctgacgaatc
     4201 gcctgtacct ggccgtccat cgcggcggaa ggggcgatga agtctgcgcc tgcagcagct
     4261 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
     4321 cacagcacac cgcagtgacc gtgagaagtg tattcacaga aacaagtgtc tgacatgacg
     4381 atcatttctg gcacagtctg cttgcagatg cgcgacatac gcgccaccag tccatcttcc
     4441 cgccaggcgt cgctgccggt ttcatcggta tggtgagaga tgccaaaagt cattacggaa
     4501 cggataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
     4561 atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
     4621 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
     4681 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
     4741 gaattaaggt gcacagtata cctgaagcgg ggcaggagtg ttttacgaaa gttgtcgcga
     4801 tgttgacaag aagagaatga aagagagacc aggacatctt tgtaatagaa atagcaataa
     4861 tttcatattc actgaaaata tttttaatct ttatttatag ctcttggtta ttattttttg
     4921 gcgtttggtt gtgctgctat aaattgaata attcaaactt tgttgcatat tgataagata
     4981 tttatctggt ttgtgttttt gatatttata acgcgtacgc atcaaaaata acattatctg
     5041 caacatactg aaatatcgtc cttttatcaa tttgatgcgt gtttttctcc ataaattata
     5101 tatgtccaca ttcggactta ggggaaagaa taattgaacc attcgtctgt aacgcagcat
     5161 aatcgttagc gcgaaacata atatgttttc tatgcattga taattgatgg atcaacttat
     5221 tacgtccctg aggagggatg acaaatgcac tcctggaaaa agaaacttgt agtatcacaa
     5281 ttagcattgg cttgcactct ggctatcacc tctcaggcta atgcagcgac caacgatatt
     5341 tctggtcaaa cttacaatac tttccatcac tacaacgacg ccacctatgc tgatgacgtt
     5401 tactatgatg gttatgtagg ctggaacaac tatgccgctg atagctatta caacggcgat
     5461 atctacccgg tcattaataa cgctaccgtt aacggcgtga tttctaccta ctatctggac
     5521 gacggtattt ctaccaatac caacgccaat agtctgacaa tcaaaaacag cactattcac
     5581 ggtatgattt attccgagtg catgacaact gattagactg gccccctgaa tctccagaca
     5641 ac
//
LOCUS       DAEEDS010000171         5612 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEEDS010000171 DAEEDS010000000
VERSION     DAEEDS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.174"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(33..311)
                     /locus_tag="KLH32_003952"
     CDS             complement(33..311)
                     /locus_tag="KLH32_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140581.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            252..437
                     /locus_tag="KLH32_003953"
     CDS             252..437
                     /locus_tag="KLH32_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140582.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(479..634)
                     /gene="hokD"
                     /locus_tag="KLH32_003954"
     CDS             complement(479..634)
                     /gene="hokD"
                     /locus_tag="KLH32_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0140583.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1286..1702)
                     /locus_tag="KLH32_003955"
     CDS             complement(1286..1702)
                     /locus_tag="KLH32_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140584.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1729..1869
                     /locus_tag="KLH32_003956"
                     /pseudo
     CDS             1729..1869
                     /locus_tag="KLH32_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1869..2909
                     /locus_tag="KLH32_003957"
     CDS             1869..2909
                     /locus_tag="KLH32_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0140585.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2937..3026)
                     /locus_tag="KLH32_003958"
     tRNA            complement(2937..3026)
                     /locus_tag="KLH32_003958"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2990..2992),aa:Ser,seq:cga)
     gene            3120..3917
                     /gene="mtfA"
                     /locus_tag="KLH32_003959"
     CDS             3120..3917
                     /gene="mtfA"
                     /locus_tag="KLH32_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBE0140586.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4018..4093
                     /locus_tag="KLH32_003960"
     tRNA            4018..4093
                     /locus_tag="KLH32_003960"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4051..4053,aa:Asn,seq:gtt)
     gene            4255..5517
                     /locus_tag="KLH32_003961"
                     /pseudo
     CDS             4255..5517
                     /locus_tag="KLH32_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1517 a         1331 c         1299 g         1465 t
ORIGIN      
        1 gaaccagaac aggtcgaagt aatacccgca cattacctcc agatccgttg ctggaatgtg
       61 caggacggac gcggtggccg ttcggaataa gggagcctga cgaagattat ccagtgacga
      121 taatcgagac tgagggcttt cttaatctcg tatccgtgtc tgcggtagcg ctgaatcagc
      181 cattcagcct gttcttcggt gcaggggtcg tgctgatacc cgtcagattt gaatgcatga
      241 gaacgccgcc cgtgcctgct ggcaggggcg gcagagttat ccgaattgta aaatttggta
      301 tcgtgcgcca tctgttttct ctgctggcgc agcaggtgcc agttgttcag gctgacggat
      361 ggattgtaaa ccagaacgac cagaaaaaac aaaacccgcc gaagcgggtt aagtgcgggt
      421 gcgttgagga tgcctgacac atcagcggtg gcgagggatt tctcccccgc cgggtctctt
      481 actcctcagg ttcgtaagct gtgaagacag cgacctccgt ctggccggtt cggattcgta
      541 cctcgcagag gtctttcctc gttaccagtg ccgtcactat gacggttaaa cagatgacga
      601 taagggcgat taacatcgcc ttttgctgct tcatagcctg cttctccttg cctttcggca
      661 cgtaagaggc taacctacat gtgttcagca tggattgagc ctcagattaa tgttaagcgt
      721 cttgcaggac gcgtaatgtt aactggggct tttctctatc tgccttttgg tgttcatgcc
      781 tgagacagat agcctcaagc acccgcagtc attctactta actaagattt ccctgcaaac
      841 cgtttttgtc cggcacagta aatatccaac taaaccaata gcgttcgctg tatttaccgc
      901 cagtattcaa tgcacatgac cgccatgaac acccctaaaa aaagggcatt tatatgtcca
      961 aacattaata tcaaaacatc aattttttcc atataccttg ctgtgaagat gatgggcata
     1021 catgatgcga acaaccagaa cgcaacaaac aaaaactgca atgcgttttt cattattccc
     1081 cctacaatca atgtgcaata acatttaaac acacctcaat ttggccggac atataaatat
     1141 ctaaaccaga aaaaatcact tacatagcgt tacaaactct ttagtctaaa tactcatcgt
     1201 aaaacattcc ccatacttat cagtccgttc cgcgccaggt agcttattgc cttatctggc
     1261 aacctataat caggtttccg ctttttcagt tggctggtcg tttaaccgac atagttaacc
     1321 cattaatctg gttgccggat gctggtggat tttcgcgttt taattgttca taaaagtgca
     1381 cagctttaac cagttcttct gatgtaaccg ggactggtgg ggcagtgaat aaggcctgaa
     1441 tttcatagtt cggcctgtcg ttacaatcct cttttttcgg tacatatttc cagtcaccaa
     1501 cccacaactc cccctgagag tccataacac cttttttcac gtagcgatat cgccacgcta
     1561 tcggctctgc ttccagcgat gcccgtgcaa ttttgaataa ctcgccctct actcgcgcca
     1621 tccctgaatt ggggtggcat ttcgtaatcg ctatttttaa tttggcttct tcgattaatt
     1681 gttcttttgt taattcagtc attttcatta ccgccctggt aatgctgcca ccagcgaaaa
     1741 tggttaatgg aaaacgggct tccgttctat accaatcgta gtggcaaacc gattgtcagc
     1801 cgggatctat ttacctgcaa taaaacttta ccaccacgcg aggtagagcc gaattttggt
     1861 gcgatctgat gggaagacga aggaaaaatc ctgaacacga aaaattacct ccaaatgtat
     1921 acccaaataa atatagttat gtatggaaac caacatccag agaatctgta acactaaccg
     1981 ccatcaagga tggtttagct gctttatgga aaaagtatga ggaaactgta aataatcgcg
     2041 atcgtgcaat gacattcggt cgcttgtggg aaaaattcct cgccagcgcc tattacagtg
     2101 acctcagtcc aagaacacaa aaagattatc tgcaacatca aaaaaagttg cttgccgtat
     2161 tcggtaaggt gccggcagat tccataaaac cagaacacat ccgtcgatac atggacaaga
     2221 gaggggaaca gagtaaaacg caagccaacc atgaaaaaag cagtatgtcc cgcgtttaca
     2281 gttgggggta tgagcgaggg tacgtgaagg ctaacccatg tgcaggtgta agtaaattca
     2341 aggccaaaaa ccgcgaacga tatgtaaccg acaaagaata ccaggcagta ttaagcgttg
     2401 cacctcttcc tgtttttatc gcaatggaaa ttgcctatct gtgtgcagcg agggtttccg
     2461 atgtgttatc gctgaaatgg gagcagattg gaaacgacgg gatctttatc cagcaaggga
     2521 aaacaggaaa aaaacagata aaagcatgga gtccacgatt acaggcggcg atcgaaaaag
     2581 caaaacagtt accaacatcc gcctatgtaa tcagcaatca atacggcaac cgatatatgt
     2641 acaaaggctt taacgaaatg tgggtagaag caagaaatcg cgcaggcaaa atttcaggta
     2701 ttttaaccga cttcaccttt catgatctga aggcgaaagg aatttcagac tatgaaggaa
     2761 gcagtcggga taagcaactt ttctctggtc acaaaaccga ggggcaagtg ctaatctatg
     2821 acaggaaggt taaagtttca ccgacacttg atgtcccgtt acctgaaaat attccaagaa
     2881 aatattccaa gtgtgatttt tgtcactgac ttaatgatgt ataagtgatt gaattttggc
     2941 ggagagaggg ggatttgaac ccccggtgga gttgccccca ctccggtttt cgagaccggt
     3001 ccgttcagcc gctccggcat ctctccgttc agatggttgt catgatgcca ggaaatttgg
     3061 cattttaaca gtccctgtcc gtgcaatttt gttcaagtga cgagtttgcg agcaaaacga
     3121 tgattaagtg gccctggaaa gtacaagaat cagcacatca aactgccctt ccctggcagg
     3181 aagcactatc gatccccctt ttaacgtgtc tgacagaaca ggaacaaagc aaattggtcg
     3241 ctcttgccga acgtttttta cagcaaaaac ggcttgttcc tttacagggc tttgagctga
     3301 attcattaag aagctgccgg atagcacttc tattttgcct gcccgttctg gagttaggac
     3361 tggaatggct ggatggtttt catgaagtct taatttatcc tgcgccattt gtggtcgatg
     3421 atgaatggga agacgatatc ggtctggtgc ataaccaacg tattgttcag tcaggtcaga
     3481 gctggcagca agggcctatc gttttgaact ggttggatat acaagattct tttgatgctt
     3541 ctggttttaa cctgattatt catgaagtcg ctcataagct ggacacccgt aacggcgatc
     3601 gcgccagcgg agttcccttt atttcgttgc gtgaggttgc tggctgggaa cacgatcttc
     3661 atgctgcaat gaacaacatt caggaagaaa tcgaattagt tggtgagaat gcggcgagca
     3721 ttgatgctta tgttgccagt gatcctgctg aatgttttgc cgtactttct gaatatttct
     3781 ttagcgcccc agaacttttt gctcctcgtt tcccttcatt gtggcaacgt ttctgtcaat
     3841 tttatcaaca agatcctttg cagagactgc atcacgctaa tgatacagac tcgttttcag
     3901 cgacgaatgt tcattaatta acaactttga agattaatta accaattgaa atggcttatg
     3961 aaatttagtg ttgacagaca aggtaccgct aagtaatatg cgccccgttc tcacgattcc
     4021 tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca
     4081 gtcagaggag ccaattttct gttttcatgc atccttgcga atctttatct gatgttgatt
     4141 caacaggtta gtgtgaaaac tcttctcggt gcattttgat tttaccctac gcatcgggaa
     4201 aaattggtgg tcaaatctgg gatcaggtta gttcgataat ggagtgaccc ccatatatcc
     4261 cttaccgacg caaaaatccg caccctcaag ccttctgata aaccctttaa agtctccgat
     4321 tctcacggtc tgtatctgct ggtcaagccg ggtggctccc gccactggta tctcaaatac
     4381 cgtattagcg gtaaagaatc ccgcattgcg ctgggtgcct atccagccat ctccctgtct
     4441 gatgcgcgac agcaacgtga aggtatccgt aaaatgctgg cgctgaatat caacccggta
     4501 cagcagcggg ctgctgaacg tggctcacga acaccggaga aagtttttaa aaacatggcg
     4561 ctggcgtgac ataaaagtaa caggaaatgg tcgcagaaca ccgccgaccg tctgcttgcc
     4621 agcctgaaca atcacatctt tccggtcatc gggaacctac ctgtatcaga acttaaaccc
     4681 cgtcatttca ttgacctgct gaaagggatc gaggaaaaag gtctgctgga ggttgcgtcc
     4741 cgcacacggc agcacctgag taacataatg cgccatgcgg tccatcagga gttaatcgat
     4801 acgaaccctg cagcaaacct tggcggcgtg accacacctc ctgtcagacg gcactatcct
     4861 gccctgccgc tggagcggct gcctgaactg cttgaacgta ttggggcata tcatcagggc
     4921 cgtgaactga cccggcatgc cgttctgctg atgctgcatg tgttcattcg ctccagtgaa
     4981 ctgcgtttcg cccgctggtc agagattgat ttcacaaacc gagtctggac gatacccgcg
     5041 acgcgagaac ccattattgg cgtgcgttat tccggccgcg gggcaaaaat gcgaatgccg
     5101 catatcgtcc ccctctcaga acagtccatc gccattctga aacagattaa ggatatcacc
     5161 ggtaataatg aactgatctt ccccggcgac cataacccgt ataagccaat gtgtgaaaac
     5221 acggtcaata aggcactgcg ggtgatgggt tacgacacga aaaaggatat ctgcggtcac
     5281 ggcttccggg caatggcatg cagtgcgctg atggaatcgg gtttatgggc aaaggacgca
     5341 gtagaacgcc agatgagtca tcaggagcgc aataccgtgc gcatggctta tattcataag
     5401 gcagagcacc tggaagcccg caaagcgatg atgcagtggt ggtcggatta tctggaagca
     5461 tgccgagaat cttatgcacc gccttataca attggtaaaa ataagtttat cccatagtat
     5521 actaacgaat cccacactct ggcaaattac caccacctct acaggaggtg gttctatata
     5581 gacttttatc aacttcatga cgaaaaacac ct
//
LOCUS       DAEEDS010000172         5559 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEEDS010000172 DAEEDS010000000
VERSION     DAEEDS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.175"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..641
                     /locus_tag="KLH32_003962"
     CDS             <1..641
                     /locus_tag="KLH32_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBE0140587.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KLH32_003963"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KLH32_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBE0140588.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="KLH32_003964"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="KLH32_003964"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBE0140589.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1379 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaaaa
//
LOCUS       DAEEDS010000173         5494 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEEDS010000173 DAEEDS010000000
VERSION     DAEEDS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.176"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            331..537
                     /locus_tag="KLH32_003965"
     CDS             331..537
                     /locus_tag="KLH32_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE0140590.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            632..1234
                     /locus_tag="KLH32_003966"
     CDS             632..1234
                     /locus_tag="KLH32_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140591.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            2434..3351
                     /locus_tag="KLH32_003967"
     CDS             2434..3351
                     /locus_tag="KLH32_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140592.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            3436..4308
                     /locus_tag="KLH32_003968"
     CDS             3436..4308
                     /locus_tag="KLH32_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBE0140593.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            4526..>5494
                     /locus_tag="KLH32_003969"
     CDS             4526..>5494
                     /locus_tag="KLH32_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HBE0140594.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNS"
BASE COUNT         1411 a         1390 c         1332 g         1361 t
ORIGIN      
        1 cggtgatatc agtctcctcc tcgccaggaa cgggctgact cactgtaact acctccgaaa
       61 acaactctgc aattatcaga acgcctgctt ctctccctgt cctcacgaaa actatcccct
      121 ctttatcgcg cgtgcgtgcg gaagcatctt ttcgcaacaa ccacccggta taccggtacg
      181 gctcagccac cgcaacctcc ccgtttatct cctggcggtc acattcccga ttatttttta
      241 cgtttctccc cggttgttat gccggtgaag gtggcgcgct gttttcatga ccacaccggc
      301 tggtttaaca acatccggag gaacattctc atggctaccc cagtttcact gatggatgac
      361 cagatggtcg acatggcatt tatcactcaa ctgaccggcc tgacagataa gtggttttac
      421 aggctcatca gggatggggc ctttccggct cccatcaagc tgggccgcag ctcccgctgg
      481 ctgaaaagtg aagtggaagc ctggctacag gcgcgtattg cacagccccg cccgtaattt
      541 ctgaccgtta tccgttcacc cgcagcagac gtaatccccg gcctgccggc ggcattctgc
      601 tgcctgttat atccccgtga ggaatatgaa aatgaaacaa cagtatcaga cccgctacga
      661 atggctccac gaaagctacc agaaatggct gaccggcttc acccggcacg ccgtatcctg
      721 gggcgtgtgt catccgaata tctactactt ccataatctg acgcccggtt gggggtcatt
      781 caatggtgaa aaaccggaga ttgccatcgt accccagagc ctgcaccggc tgatttatgg
      841 ccctgacaaa cgggccacgc cgcccctgga tgatgatctg atagtgaatt tatgcaccag
      901 tgaacatctg ctggttcatc atccgatgct ggaaggcatt ctcctgtctg aatgtgaacg
      961 tctgaggcag cgttctctgg caaataaact gatcagcctc ttccgtcagt ttggtggcac
     1021 ggagttgcgc ctcaaactgg tctggctttg ctggcttgat ttaatgaccg gaaacagcct
     1081 tgaagactgg aaagagaacc tgaaacggaa atcagaaaaa gagctggagg aatggatcat
     1141 caaccgtcag aggcagagcg cagcactgac ggatctgatg gatcagtacg tgctcctggc
     1201 ataccgcaca acggttgacg atagccgcaa ctgatgccgg ttgcttccgg aaggtgtgac
     1261 aaaatacgga aatgatgatg ctcccggccc ccatgctcac ctgcagagcg tgttcgtcgt
     1321 catcatactg agccatgacg ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca
     1381 ccaccagagg agccacggac agggatgttc tttacagccc gatacacgcc aggttctcag
     1441 cagactgaat gtgcaggtgc ggttattcac cagaaatggc atgctgcggt acatacccat
     1501 cctggtgaca atatgtacgt ggcgacagcg atcccggcac tgtggaagag actcactcag
     1561 cctgcaggat attctttccg gcatccggga atgtccgcct gaatgcaaac aggatgatat
     1621 ttgaatccgt aatactgttc tttcatcata aataatttat gcagatacag ttaaccgtta
     1681 cagtagccgg tttgttttac cgggaaataa tatccgtcag actgataccg gaacatatct
     1741 gaaacacgtt atgatgtttt gttatcagaa atgttgtata ttcttatccg tggcgacagt
     1801 ttaatgccct ctggtatcac ctgataatgt atatcctgcg atgattcgtt atatttacgg
     1861 gacacgcctc ttttcattgt gctccttttc agacttcatt ttccgtaagt gttccccggc
     1921 ataaaggagg tacctttctg gcgtttataa ctctctttat cggtgacgcc ggtaatacag
     1981 ggaataccac accgacaaac gaggtattaa cagacgatat ttcagtgtcc ataatttttc
     2041 tgtccggtta aataacgtca gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc
     2101 ataccgggtt acaacgtaaa aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac
     2161 atgtttacct ttcaataccc cctgtctgca gtctccactc ttccatcctc ttaagctacg
     2221 tcggccacat gagtgcactt ccatgataac aggtattcct gtcagcataa cgaagccggt
     2281 taccttccct tcctcctgca tttcctgtca gaatgactat acagcgcgca gattgccttt
     2341 ttattgacca tcttaatata ccgttataaa cttcttttta tcatattatt atcaccttat
     2401 tatatttaat ttttaagcag tcggagaaag aggatggttc agaaaatttt gtcggataag
     2461 gttatgaatg aaagaacaaa tgcgtattac tcttattatc ttggagagcg taatatctcc
     2521 gtactgcccc ttaatgttta tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac
     2581 agagaaaatc tgaacatcac actttcagat tttgaacttg aacaaataat ttctggtatg
     2641 cgtctgaagg ccctggcctt cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt
     2701 gaacgcgcat gcttattttc ctggtatctg ttgatgcagt ttatccagaa taacagggca
     2761 aaaataagcg cagacttgct tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa
     2821 gggaatgcct tcccgtcgga ctcatcgaca cagttcagac aaatactcag agttctcgat
     2881 attctttctg ataagaattt gcgggatgag tggataatcc agaccaaaga cagatggatg
     2941 cgggcattta aatcaaagag tcctttcagt tacctcctcc cggagaacga acatgaatgc
     3001 atatggacat ggaattattt gaagggaaaa aatattgcac tggaaaaact ggccagtttt
     3061 cctggttctg ctgatattta ccacgcaatt cacctttcat ttgacatatg ggttacctgt
     3121 ccactcacct cacctgacga tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg
     3181 cagcgcaagt acaaaaaaat gcaggaagat aaagtcaatg tacagttttt tcttgacgca
     3241 gaaaccaggg cgcagctcaa agaattatcg agggtcagaa gactcagtac cggcgaaatg
     3301 ctgcatgacc tgattgttga agagtataaa aggtaccgac attccagata atttcagaca
     3361 tatattatct ccgtgagtcc cccgccacct ttccggtgtg cgggggtttt gtcttttttc
     3421 accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa accgctctcc
     3481 tccctgcctt actcactttc ccgtcacatc ctggaacatc tgcgcaaact caccagtcac
     3541 gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact ctgtaatgca
     3601 ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg ggaagtgcag
     3661 cgtttccgtc tgagtggcca tggtcacagc atggttatca ctgacctgcc cggggtgggc
     3721 gagagccggg acagagatgc agagtatgaa gccctgtacc gtgacattct gcctgaactt
     3781 gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga tgagtatttc
     3841 tggcgacaca tcctgcaccg gggacatcag caggtgctgt ttgtggtgac gcaggccgac
     3901 aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc cgcacaggca
     3961 cagaatattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt acatccggtt
     4021 gttgccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc gctaatgaca
     4081 gcacttcccg accatgccgc cagtcccctg atgacccgcc tgcaggacgg gctgcgcacg
     4141 gaatctgtcc ggggtcaggc ccgtgaacag tttaccggtg cggtggaccg gatatttgac
     4201 acggcggaga gcgtctgtgt tgcctctgtt gcccgtaagg ttctgcgcgc tgttcgtgac
     4261 accgtggtct ctgttgcccg cgcggtatgg aactggattt tcttctgaac ccggtggtga
     4321 tgcaccagac tcttgttaca gatttttaac cccatgaaaa cacaatgcaa acacagccat
     4381 acccgggttg tccccttgcg ttgtgtttct caagttaata gaatgcatcg atcgttacta
     4441 tcatatcgat cgctataaac gatcgtttgt ggtaaatgac ctcctccgtg gttgtcatga
     4501 aaatgcgtct caggaaggtt aaataatgca cgaaaagaac atcgccctgc tttgtgatga
     4561 agccgaccga cttttgcaac tgaacattaa tctgctccgg caaatggttg atgaaccgga
     4621 tgtgttatct gacagtaaga acgaaaacag actgcttttt gataaacaga aagcactgaa
     4681 aagaattgag gagctggagg gcgaacaaat caaaaccgcc cgcagggaga tggtgctggc
     4741 tgttgtcggc acgatgaaag caggtaaatc aactaccatc aacgccattg tggggcagga
     4801 aattctgcct aaccgtaacc gccccatgac ctctgtaccg acgctcatcc gccacgttcc
     4861 cggaaaaact gagccggtcc tccatctgga acatattcag cccgtccgca atttattaag
     4921 cacactgcaa caaaaactcg acaccccggc aggacagcaa gtcgcacaga ccctgcagca
     4981 aaccggggat acctgcgaac tgctggatat tctggcaaat gatggctggc tcaaaaatga
     5041 ataccacggg gaagacgaaa tctttaccgg actggcatcg ttaaacgatc tggttcgcct
     5101 tgctgcggca atggggagtg agtttccttt tgatgaatac gcagaagtgc agaaactgcc
     5161 ggtgatcgac gtggaattca gccatctggt ggggatggat gcatgccagg gaacactcac
     5221 actgctagat acccccggcc ctaatgaggc cggacaaccg cagatggaag tgatgatgcg
     5281 ggatcaactg cagaaagcct ctgcggtgct ggcggtgatg gattacaccc agatgaactc
     5341 aaaagcggat gaagacgtcc gtaaagagct taatgccatt gctgacgtat cagccggccg
     5401 cctgtttgtg ctggtcaata aatttgatga gaaagaccgc aatggcgatg gggcagatgc
     5461 cgtacgccag aaagttccgg caatgctgaa cagc
//
LOCUS       DAEEDS010000174         5417 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEEDS010000174 DAEEDS010000000
VERSION     DAEEDS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.177"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..511
                     /locus_tag="KLH32_003970"
     CDS             <1..511
                     /locus_tag="KLH32_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HBE0140595.1"
                     /translation="RGAYNEIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQ
                     TRLHLIANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDM
                     AAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGM
                     DMSHAESAH"
     gene            533..2524
                     /gene="cyoB"
                     /locus_tag="KLH32_003971"
                     /pseudo
     CDS             533..2524
                     /gene="cyoB"
                     /locus_tag="KLH32_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2514..3128
                     /locus_tag="KLH32_003972"
     CDS             2514..3128
                     /locus_tag="KLH32_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBE0140596.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3128..3457
                     /locus_tag="KLH32_003973"
     CDS             3128..3457
                     /locus_tag="KLH32_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBE0140597.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3469..4359
                     /gene="cyoE"
                     /locus_tag="KLH32_003974"
     CDS             3469..4359
                     /gene="cyoE"
                     /locus_tag="KLH32_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBE0140598.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4544..4855
                     /locus_tag="KLH32_003975"
     CDS             4544..4855
                     /locus_tag="KLH32_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBE0140599.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4839..4997
                     /locus_tag="KLH32_003976"
     CDS             4839..4997
                     /locus_tag="KLH32_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBE0140600.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4990..5388
                     /locus_tag="KLH32_003977"
     CDS             4990..5388
                     /locus_tag="KLH32_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0140601.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1183 a         1346 c         1430 g         1458 t
ORIGIN      
        1 tcggggcgct tacaatgaaa tcgctttccc ggcgaacact ccggtgtact tcaaagtgac
       61 ctccaactcc gtgatgaact ccttcttcat tccgcgtctg ggtagccaga tttatgccat
      121 ggccggtatg cagactcgcc tgcatctgat cgccaacgaa cccggtactt atgacggtat
      181 ctccgccagc tacagcgggc cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc
      241 ggatcgcgcc gaattcgacc aatgggtcgc aaaagcgaaa cagtcgccga ataccatgtc
      301 tgacatggct gcgttcgaaa aactggccgc gcctagcgaa tacaaccagg tggaatattt
      361 ctccaacgtg aaaccagact tgtttgccga tgtgattaac aagtttatgg ctcacggtaa
      421 gagcatggac atgacccagc cagaaggtga gcacagtgca cacgaaggta tggaaggcat
      481 ggacatgagc cacgcggaat ccgcccatta aaggggttga ggaagaataa agatgttcgg
      541 aaaattatca cttgatgcag tcccgttcca tgaaactatc gtcatggtta cgatcgctgg
      601 cattattttg ggaggtctgg cgctcgttgg cctgatcact tacttcggta agtggaccta
      661 cctgtggaaa gagtggctga cctccgtcga ccataaacgc ctcggtatca tgtatatcat
      721 cgtggcgatt gtgatgttgc tgcgtggttt tgctgacgcc attatgatgc gtagccagca
      781 ggctcttgcc tcggcgggtg aagcgggctt cctgccacct caccactacg atcagatctt
      841 caccgcgcac ggcgtgatta tgatcttctt cgtggcgatg cctttcgtta tcggtctgat
      901 gaacctggtg gttccgctgc agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa
      961 cttaagcttc tggtttactg ttgttggcgt gattctggtt aacgtttctc tcggcgtggg
     1021 cgaatttgcg cagaccggct ggctggccta tccaccgcta tcgggaatag agtacagtcc
     1081 gggagtcggt gttgattact ggatatggag tctccagcta tccggtatag gtacgacgct
     1141 taccggtatc aacttcttcg ttaccattct gaagatgcgc gcaccgggca tgaccatgtt
     1201 caagatacca gtatttacct aggcatcact gtgcgcgaac gtactgatta ttgcttcctt
     1261 cccaattctg acggttaccg tcgcgttgtt gaccctggat cgctatctgg gcacccattt
     1321 ctttaccaac gatatgggtg gcaacatgat gatgtacatc aacctgattt gggcctgggg
     1381 ccacccggaa gtttacatcc tgatcctgcc tgttttcggt gtgttctccg aaattgcggc
     1441 aaccttctcg cgtaaacgtc tgtttggtta tacctcgctg gtatgggcaa ccgtctgcat
     1501 caccgtgctg tcgttcatcg tttggctgca ccacttcttt acgatgggtg cgggcgcgaa
     1561 cgtaaacgcc ttctttggta tcaccacaat gattatcgcc atcccgaccg gggtgaagat
     1621 cttcaactgg ctgttcacca tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg
     1681 gactatcggt tttatcgtca ccttctcggt gggcggtatg actggcgtgc tgctggcagt
     1741 accgggcgcg gacttcgttc tgcataacag cctgttcctg attgcgcact tccataacgt
     1801 gatcatcggc ggcgtggtct tcggctgctt cgcagggatg acctactggt ggcctaaagc
     1861 gttcggtttc aaactgaacg aaacctgggg taaacgcgcg ttctggttct ggatcatcgg
     1921 cttcttcgtt gcctttatgc cgctgtatgc gctgggcttc atgggcatga cccgtcgttt
     1981 gagccagcag attgacccgc agttccacac catgctgatg attgcagcca gcggtgcagt
     2041 actgattgcg ctgggtattc tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg
     2101 cgaccagaac cgtgacctga ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac
     2161 ctcttccccg cctccgttct ataactttgc cgtagtgccg cacgttcacg aacgtgatgc
     2221 attctgggaa atgaaagaga aaggcgaagc gtataaaaag cctgaccact atgaagaaat
     2281 tcatatgccg aaaaacagcg gtgcaggtat cgtcattgca gctttctcca ccatcttcgg
     2341 tttcgccatg atctggcata tctggtggct ggcgattgtt ggcttcgcag gcatgatcat
     2401 cacctggatc gtgaaaagct tcgacgagga cgtggattac tacgtgccgg tggcagaaat
     2461 cgaaaaactg gaaaaccagc atttcgatga gattactaag gcagggctga aaaatggcaa
     2521 ctgatacttt gacgcacgcg actgcccacg cgcacgaaca cgggcaccac gatgcaggcg
     2581 gaaccaaaat cttcggattt tggatctacc tgatgagcga ctgcattctg ttctcaatct
     2641 tgtttgctac ctatgccgtt ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca
     2701 ttttcgaact gccgttcgtt ctggttgaaa ctttcttgct gttgttcagc tccatcacct
     2761 acggcatggc ggctatcgcc atgtacaaaa acaacaaaag ccaggttatc tcctggctgg
     2821 cgttgacctg gttgtttggt gccggattta tcgggatgga aatctatgaa ttccatcacc
     2881 tgattgttaa cggcatgggg ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg
     2941 tcggcacgca cggtctgcac gtcacttctg gtcttatctg gatggcggtg ctgatggtgc
     3001 aaatcgcccg tcgcggcctg accagcacta accgtacccg catcatgtgc ctgagcctgt
     3061 tctggcactt cctggatgtg gtttggatct gtgtgttcac tgttgtttat ctgatggggg
     3121 cgatgtaatg agtcattcta ccgatcacag cggcgcgtcc catggcagcg taaaaaccta
     3181 catgacaggc tttatcctgt cgatcattct gacggtgatt ccgttctgga tggtgatgac
     3241 aggggctgcc tctccggccg taattctggg aacaatcctg gcaatggcag tggtacaggt
     3301 tctggtgcat ctggtgtgct tcctgcacat gaataccaaa tcagatgaag gctggaacat
     3361 gacggcgttt gtcttcaccg tgctaatcat cgctatcctg gttgtaggct ccatctggat
     3421 tatgtggaac ctcaactaca acatgatgat gcactaagag cggcggttat gatgtttaag
     3481 caatacctgc aagtaacgaa accaggcatc atctttggca acctgatctc ggtgattggg
     3541 ggattcctgc tggcctcaaa gggcagcatt gattatcccc tgtttatcta cacgctggtt
     3601 ggggtgtcac tggttgtgac gtcgggttgt gtgtttaaca actacatcga cagggatatc
     3661 gacagaaaga tggaaaggac gaagaatcgg gtgctggtta aaggcctgat ctctcctgct
     3721 gtctcgctgg tgtacgccac cttgctgggt attgctggct ttatgctgct gtggtttggc
     3781 gcgaatccgc tggcctgctg gctgggggtg atgggctttg tggtttatgt cggcgtctat
     3841 agcctgtaca tgaaacgcca ctcagtctac ggcacattga ttggttcgct ctccggcgct
     3901 gcgccgtcgg tgattggcta ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc
     3961 ctgctggcta tcttcagcct gtggcagatg cctcactcct atgccatcgc cattttccgc
     4021 tttaaggatt accaggcggc aaacattccg gtattgccag tggtaaaagg catttcggtg
     4081 gcgaagaatc acatcacgct gtatatcatc gcctttgccg ttgccacgct gatgctctct
     4141 cttggcggtt acgctgggta taaatatctg gtggtcgccg cggcggttag cgtctggtgg
     4201 ttaggtatgg ctctgcgcgg ttataaagtt gctgatgaca gaatctgggc gcgcaagctg
     4261 ttcggcttct ctatcatcgc catcactgcc ctctcggtga tgatgtccgt tgattttatg
     4321 gtaccggact cgcatacgct gctggctgct gtgtggtaac aaaacctctc tattaaaaag
     4381 gtgctacggc acctttttta ttgtctgaga tcagagatac ggctaataac cgtatctctt
     4441 gacagagtga gcagatgcac ataaattaat caaacggttg atagccgtat tatgtatgag
     4501 atcgactccc tacgatttca ttcagttaat caaagaagtc ataatgcctg gaaaaacagc
     4561 cgctctatac gatgtcgata aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc
     4621 cgacgccaaa aacaaacttc gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt
     4681 tattttgagc gctgctatgg aacgcgccga aagtgtgctt gataaccaac gtcgccgtga
     4741 gctttcgaat caaagctggg aactgatgaa ccaactcatc gctgaacctg ctcaaccgac
     4801 gctcgccctc aaggcgttaa tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga
     4861 ttactctgcc catagtcctc tcctgtaatt atcagtctga tattacttat cctgggcaaa
     4921 aacagtttga ttgcggtaac cctgttatcg ataaatttgt acgcgctacg cgcatcgcta
     4981 aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac ttattgacag acaaagtggt
     5041 gaactgattg gcatctgtac ttttacggca tattcgctgg aaagacaacg cgtttctggc
     5101 gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca gattagtcat gttgggggga
     5161 gcgcggaagt atcaaaagcg gggctttggt caggacctac tatgtgattt ttttgaacat
     5221 gtaaaaaaaa ttcacccggc attaccaatt aaaggggttt atcttgatgc tgaccctgcc
     5281 gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt
     5341 ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc
     5401 acccgccagg gcaagtt
//
LOCUS       DAEEDS010000175         5360 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEEDS010000175 DAEEDS010000000
VERSION     DAEEDS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.178"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            12..398
                     /locus_tag="KLH32_003978"
     CDS             12..398
                     /locus_tag="KLH32_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBE0140602.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            406..1092
                     /locus_tag="KLH32_003979"
     CDS             406..1092
                     /locus_tag="KLH32_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE0140603.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1070..1693)
                     /gene="tetR(B)"
                     /locus_tag="KLH32_003980"
     CDS             complement(1070..1693)
                     /gene="tetR(B)"
                     /locus_tag="KLH32_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBE0140604.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1775..2980
                     /gene="tet(B)"
                     /locus_tag="KLH32_003981"
     CDS             1775..2980
                     /gene="tet(B)"
                     /locus_tag="KLH32_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBE0140605.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3093..3686)
                     /gene="tetC"
                     /locus_tag="KLH32_003982"
     CDS             complement(3093..3686)
                     /gene="tetC"
                     /locus_tag="KLH32_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBE0140606.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3819..4190
                     /locus_tag="KLH32_003983"
     CDS             3819..4190
                     /locus_tag="KLH32_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0140607.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4200..>5360)
                     /locus_tag="KLH32_003984"
     CDS             complement(4200..>5360)
                     /locus_tag="KLH32_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBE0140608.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHG
                     RSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLG
                     WYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKS
                     RSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIE
                     ETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQA
                     NTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1464 a          997 c         1174 g         1725 t
ORIGIN      
        1 ggacaaggat aatgtttgat gttcacgttg ttttagataa tcaaatagga caattagcat
       61 tactaggaaa aacattaggt aataaaggta ttggattgga agggggaggg atatttacgg
      121 ttggtgatga atgccatgct cattttcttg ttgaacaagg aaaggaagct aaaatagcgc
      181 tagagcaagc tggactgtta gtacttgcga tccggacacc attaattcgt aagttaaaac
      241 aggaaaaacc gggggaactt ggcgaaatag cacgagtatt ggcggagaat aacattaata
      301 ttttagtgca atacagtgac catgctaacc aactgatatt aataacggac aatgatagta
      361 tggctgcatc tgttacgctc ccttgggcaa taaagtgaac ttgcgatggc taatttaata
      421 cgaaaagagg ttacctttga gtcctcaata gccgcgatag gggcggctat gtctgacatt
      481 tcacgagtta aaatactcag tgctttgatg gatgggcgag cttggacggc cactgagcta
      541 agttctgtgg cgaatatatc agcttcaacg gcgagcagtc atttatctaa attattagat
      601 tgccagctaa tcacagtagt agctcaaggc aagcatcgtt attttcggct agcaggaaaa
      661 gatattgctg aattgatgga aagtatgatg gggatctcct taaaccatgg cgtacatgcc
      721 aaagtttcca cgccagtgca tttacgaaaa gcacgtactt gctatgatca tttagctggc
      781 gaagttgccg ttaagatcta tgattccctt tgtcaacagc aatggatcac tgaaaatggt
      841 tcaatgatca cattaagtgg tattcaatat tttcatgaaa tgggaattga cgttccttcc
      901 aaacattcac gtaaaatctg ttgtgcgtgt ttagattgga gtgaacgccg tttccattta
      961 ggtgggtacg ttggagccgc attattttcg ctttatgaat ctaaagggtg gttaactcga
     1021 catcttggtt accgtgaagt taccatcacg gaaaaaggtt atgctgcttt taagacccac
     1081 tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg
     1141 ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac
     1201 tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata
     1261 cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa
     1321 aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag
     1381 gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac
     1441 ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag
     1501 tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta
     1561 catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta
     1621 aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat
     1681 ctaatctaga catcattaat tcctaatttt tgttgacact ctatcattga tagagttatt
     1741 ttaccactcc ctatcagtga tagagaaaag tgaaatgaat agttcgacaa agatcgcatt
     1801 ggtaattacg ttactcgatg ccatggggat tggccttatc atgccagtct tgccaacgtt
     1861 attacgtgaa tttattgctt cggaagatat cgctaaccac tttggcgtat tgcttgcact
     1921 ttatgcgtta atgcaggtta tctttgctcc ttggcttgga aaaatgtctg accgatttgg
     1981 tcggcgccca gtgctgttgt tgtcattaat aggcgcatcg ctggattact tattgctggc
     2041 tttttcaagt gcgctttgga tgctgtattt aggccgtttg ctttcaggga tcacaggagc
     2101 tactggggct gtcgcggcat cggtcattgc cgataccacc tcagcttctc aacgcgtgaa
     2161 gtggttcggt tggttagggg caagttttgg gcttggttta atagcggggc ctattattgg
     2221 tggttttgca ggagagattt caccgcatag tccctttttt atcgctgcgt tgctaaatat
     2281 tgtcgctttc cttgtggtta tgttttggtt ccgtgaaacc aaaaatacac gtgataatac
     2341 agataccgaa gtaggggttg agacgcaatc gaattcggta tacatcactt tatttaaaac
     2401 gatgcccatt ttgttgatta tttatttttc agcgcaattg ataggccaaa ttcccgcaac
     2461 ggtgtgggtg ctatttaccg aaaatcgttt tggatggaat agcatgatgg ttggcttttc
     2521 attagcgggt cttggtcttt tacactcagt attccaagcc tttgtggcag gaagaatagc
     2581 cactaaatgg ggcgaaaaaa cggcagtact gctcggattt attgcagata gtagtgcatt
     2641 tgccttttta gcgtttatat ctgaaggttg gttagttttc cctgttttaa ttttattggc
     2701 tggtggtggg atcgctttac ctgcattaca gggagtgatg tctatccaaa caaagagtca
     2761 tcagcaaggt gctttacagg gattattggt gagccttacc aatgcaaccg gtgttattgg
     2821 cccattactg tttgctgtta tttataatca ttcactacca atttgggatg gctggatttg
     2881 gattattggt ttagcgtttt actgtattat tatcctgcta tcgatgacct tcatgttaac
     2941 ccctcaagct caggggagta aacaggagac aagtgcttag ttatttcgtc accaaatgat
     3001 gttattccgc gaaatataat gaccctcttg ataacccaag agggcatttt ttacgataaa
     3061 gaagatttag cttcaaataa aacctatcta ttttatttat ctttcaagct caataaaaag
     3121 ccgcggtaaa tagcaataaa ttggcctttt ttatcggcaa gctcttttag gtttttcgca
     3181 tgtattgcga tatgcataaa ccagccattg agtaagtttt taagcacatc atcatcataa
     3241 gctttaagtt ggttctcttg gatcaatttg ctgacaatgg cgtttacctt accagtaatg
     3301 tattcaaggc taattttttc aagttcattc caaccaatga taggcatcac ttcttggata
     3361 gggataaggt ttttattatt atcaataata taatcaagat aatgttcaaa tatactttct
     3421 aaggcagacc aaccatttgt taaatcagtt tttgttgtga tgtaggcatc aatcataatt
     3481 aattgctgct tataacaggc actgagtaat tgttttttat ttttaaagtg atgataaaag
     3541 gcacctttgg tcaccaacgc ttttcccgag atctcatcta ttgaaacagc ttgatagcct
     3601 ttttcaacaa acaatattcg tgctgagtta accagtgatt gataggtact cttaaaattt
     3661 tcttgttgat gatttttatt ttccatgata gatttaaaat aacataccgt cagtatgttt
     3721 atggtatcat gatgatgtgg tcgtgacaat cttaagaaca tttaggttat tttatgtata
     3781 ttgaacagca ttctcgctat caaaataaag ctaataacat ccaattaaga tatgatgata
     3841 agcagtttca tacaacggtt atcaaagatg ttctattatg gattgaacat aatttagatc
     3901 agtctttact gcttgatgat gtggcgaata aagcgggtta taccaagtgg tattttcagc
     3961 ggctgttcaa aaaagtaaca ggggtcacac tggctagcta tattcgtgct cgtcgtttga
     4021 cgaaagcggc tgttgagttg aggttgacga aaaaaactat ccttgagatc gcattaaaat
     4081 atcaatttga ttcccaacaa tcttttacac gtcgatttaa gtacattttt aaggttacac
     4141 caagttatta tcggcgtaat aaattatggg aattggaggc aatgcactga gagatcccct
     4201 cataatttcc ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc
     4261 acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
     4321 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
     4381 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
     4441 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg
     4501 taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata
     4561 ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag aacccatggc
     4621 tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag
     4681 cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga
     4741 ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt
     4801 aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt
     4861 actcgactta accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag
     4921 ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct
     4981 agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa
     5041 agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag tcgtttttgc
     5101 tcacgaatat cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag
     5161 ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat
     5221 cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt
     5281 tcggtaagag tgagagtttt acagtcaagt aatgcgtggc aagccaacgt taagctgttg
     5341 agtcgtttta agtgtaattc
//
LOCUS       DAEEDS010000176         5339 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEEDS010000176 DAEEDS010000000
VERSION     DAEEDS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.179"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="KLH32_003985"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="KLH32_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBE0140609.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="KLH32_003986"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="KLH32_003986"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBE0140610.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="KLH32_003987"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="KLH32_003987"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBE0140611.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="KLH32_003988"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="KLH32_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBE0140612.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1456 g         1283 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtt cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAEEDS010000177         5221 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEEDS010000177 DAEEDS010000000
VERSION     DAEEDS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.180"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..138)
                     /locus_tag="KLH32_003989"
     CDS             complement(<1..138)
                     /locus_tag="KLH32_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140613.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YT"
     gene            245..319
                     /gene="tnaC"
                     /locus_tag="KLH32_003990"
     CDS             245..319
                     /gene="tnaC"
                     /locus_tag="KLH32_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBE0140614.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            540..1955
                     /gene="tnaA"
                     /locus_tag="KLH32_003991"
     CDS             540..1955
                     /gene="tnaA"
                     /locus_tag="KLH32_003991"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBE0140615.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            2046..3293
                     /gene="tnaB"
                     /locus_tag="KLH32_003992"
     CDS             2046..3293
                     /gene="tnaB"
                     /locus_tag="KLH32_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBE0140616.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3425..4600
                     /gene="mdtL"
                     /locus_tag="KLH32_003993"
     CDS             3425..4600
                     /gene="mdtL"
                     /locus_tag="KLH32_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBE0140617.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGIMLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4575..>5221
                     /gene="yidZ"
                     /locus_tag="KLH32_003994"
     CDS             4575..>5221
                     /gene="yidZ"
                     /locus_tag="KLH32_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBE0140618.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1204 a         1252 c         1305 g         1460 t
ORIGIN      
        1 ggtgtaagtg aactgtagtt gccatgtttt acggcaatga gagcagagat agcgctgatg
       61 tccggcagtg cttttgccgt tacgcaccac gccttcagta gcggagcagg aaggacatct
      121 gatggaaatg gaagccacgc aagcacctta aaatcaccat catacactaa atcagtaagt
      181 tggcagcatt accaaattct gagtgtaata atgtagcggc gtgtctttcg aggataagtg
      241 cattatgaat atcttacatg tatgtgtgac ctcaaaatgg ttcaatatcg acaataagat
      301 tgtcgatcac cgcccttaat ttacccttct gtagccatca ccagagccaa accgattaga
      361 ttcaatgtga tcgattagtt tgctatatct taattttgcc ttttgcaaag gccatctctc
      421 gtttatttac ttattttagt aaatgatggt gcttgtatat atacatggtg aattaatgga
      481 tattgcagat gtaatattca cagggatcac tgtaattaaa ataaatgaag gattatgtaa
      541 tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag ccagtaaaac
      601 gtactactcg cgcttatcgt aaagaagcaa ttattaaatc cggtatgaac ccgttcctgc
      661 tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg gcggtaaccc
      721 aaagtatgca ggcagcgatg atgcgcggcg acgaagccta cagcggcagc cgcagctact
      781 atgcgttagc cgagtcagtg aaaaatatct ttggttatca atatactatt ccgactcacc
      841 agggccgtgg cgcagagcaa atctatattc cggtactgat taaaaaacgc gagcaggaaa
      901 aaggcctgga tcgcagcaaa atggtggcat tctctaacta tttctttgat accacgcagg
      961 gccatagcca gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa gccttcgata
     1021 cgggcgtgcg ttacgacttt aaaggcaact ttgacctcga aggattagaa cgcggtattg
     1081 aagaagttgg cccgaataac gtgccgtata tcgttgcaac catcaccagt aactctgcag
     1141 gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg aagaaatacg
     1201 atattccggt ggtaatggac tccgcacgct ttgctgaaaa cgcctatttc atcaagcagc
     1261 gtgaagcaga atacaaagac tggaccatcg agcagatcac ccgcgaaacc tacaaatatg
     1321 ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt gccgatgggc ggcttgctgt
     1381 gcatgaaaga cgacagcttc tttgatgtgt acaccgagtg cagaaccctt tgcgtggtgc
     1441 aggaaggctt cccgacatat ggcggcctgg aaggcggcgc gatggagcgt ctggcggtag
     1501 gtctgtatga cggcatgaat ctcgactggc tggcttatcg tatcgcgcag gtacagtatc
     1561 tggtcgatgg tctggaagag attggcgttg tctgccagca ggcgggcggt cacgcggcat
     1621 tcgttgatgc cggtaaactg ctgccgcata tcccggcaga ccagttcccg gcacaggcgc
     1681 tggcgtgcga gctgtataaa gtcgccggta tccgtgcggt agaaattggc tctttcctgt
     1741 taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg ctgcgtttaa
     1801 ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa gcctttaaac
     1861 atgtaaaaga gaacgcggcg aatattaaag gattaacctt tacctacgaa ccaaaagtat
     1921 tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat actacagagt ggctataagg
     1981 atgttagcca ctctcttacc ctacatcctc aataacaaaa atagccttcc tctaaaggtg
     2041 gcatcatgac tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt atggttatag
     2101 caggtacagt aattggtgga ggtatgtttg ctttacctgt tgatcttgcc ggtgcctggt
     2161 ttttctgggg tgcctttatc cttatcattg cctggttttc aatgcttcat tccgggttat
     2221 tgttattaga agcaaattta aattatcctg tcggctccag ttttaacacc atcaccaaag
     2281 atttaatagg taacacctgg aacattatca gcggtattac cgttgccttc gttctctata
     2341 tcctcactta tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg atatcaatga
     2401 atttgggcta tcacgctaat ccacgtattg tcgggatctg cacagccatt ttcgttgcca
     2461 gcgtattgtg gataagttcg ttagccgcca gtcgcattac ctcattgttc ctcgggctga
     2521 agattatctc ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat tactccatcc
     2581 tgcgcgatgc caccagcacc actgcgggaa cgtcttactt cccgtatatc tttatggctt
     2641 tgccggtgtg tctggcgtca tttggtttcc acggcaatat tcccagcctg attatttgct
     2701 atggcaaacg caaagataag ttaatcaaaa gcgtggtatt tggttcgctg ctggcgctgg
     2761 tgatttatct cttctggctc tattgcacga tggggaatat tccgcgcgaa agctttaagg
     2821 cgataatctc ctcaggcggc aacattgatt cgctggtgaa atcgttcctc ggcaccaaac
     2881 agcacggcat tatcgagttt tgcctgctgg tgttctctaa cttagctgtt gccagttcgt
     2941 tctttggtgt cacgctgggg ttgttcgatt atctggcgga cctgtttaag attgataact
     3001 cccacggcgg gcgtttcaaa accgtgctgt taaccttcct gccacctgcg ttgttgtatc
     3061 tgatcttccc gaacggcttt atttacggga tcggcggtgc cgggctgtgc gccaccatct
     3121 gggcggtcat tattcccgca gtgcttgcaa tcaaagctcg caagaagttt cccaatcaga
     3181 tgttcacggt ctggggcggc aatcttattc cggcgattgt cattctcttt ggtataaccg
     3241 tgattttgtg ctggttcggc aacgtcttta acgtgttacc taaatttggc taaatccttc
     3301 aagaagccag ccattcgctg gcttcttgcc tctcaggaaa tcacttatgt ccaaatggca
     3361 actcgcctta tcctccttca ccacgtatgc tttgcgtcac cttactatca ggacgcttta
     3421 gcccatgtcc cgctttttga tttgtagttt tgccctggtt ttactttatc ccgccgggat
     3481 tgatatgtac ctcgttggtt taccgcgcat cgccgccgat ctcaatgcca gcgaagcgca
     3541 gttgcatatt gcgttctccg tatatctggc ggggatggca gctgcgatgc tatttgccgg
     3601 taaagtggcc gatcgttcag ggagaaagcc ggtcgccata cccggcgcgg cgctatttat
     3661 tattgcctcg gtgttctgtt cactggctga aaccagcacg ttatttcttg caggccgatt
     3721 tctacagggg ttgggcgcag gctgttgtta cgtggtggcg ttcgccattt tgcgcgacac
     3781 gctggatgat cgacgtcggg ctaaagtgct gtcattactc aacggtatta cctgcatcat
     3841 tccggtgtta gcgccagtgc tcggacatct gattatgctt aaattcccgt ggcagagtct
     3901 gttctgggcg atggcaatga tgggcatcgc ggtactgatg ttgtctttgt ttattttaaa
     3961 agaaacgcgc ccagcgtccc ccgctgcttc ggacaaacca cgagaaaata gcgagtcgct
     4021 gcttaatcgt tttttcctca gccgtgttgt tatcaccacc ctcagcgttt cggtgatcct
     4081 cactttcgtc aatacatcgc cggtattgct gatggaaatc atggggtttg agcgcggaga
     4141 atacgccacc attatggcgc tgaccgctgg cgtcagcatg accttttcat tctcgacgcc
     4201 atttgcgctg ggaattttta agccacgtac gttgatgatc acctcgcagg tgttattcct
     4261 tgcggcgggg atcacccttg ccgtttcacc ttcccatgcg gtttctctgt ttggtatcat
     4321 gctgatttgc gccggtttct cggtaggttt tggcgtagcg atgagtcagg cgttaggacc
     4381 attttcatta cgcgcgggcg tagccagctc gaccttaggt attgcgcagg tttgcggttc
     4441 gtcactgtgg atttggctgg ccgcggtggt tggtatcggc gcatggaata tgctgatcgg
     4501 gattctgatt gcctgtagca tagtgagcct gttgctgatt atgttcgtcg cgcctggacg
     4561 ccccgttgcc gctcatgaag aaatccatca ccacgcttga tctcaatctg ctgctctgtc
     4621 tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc gaagcggatg aacgtgaccc
     4681 cttcagcggt gagtaagtcg ctggcaaagt taagagcgtg gtttgacgac ccgctctttg
     4741 tgaactcacc gctggggctg tcgcccacac agctaatggt cagcatggag caaaatctgg
     4801 cggaatggat gcaaatgagc aatctgctgc tggataaacc gcaccaccag acaccgcgcg
     4861 gcctgaagtt tgagttggcg gcggaatcgc cgctgatgat gatcatgctt aatgcgctgt
     4921 cgaaacggat ctaccaacgt tacccgcagg cgaccatcaa actacgtaac tgggattacg
     4981 attccttaga tgccattact cgtggtgaag tggatattgg tttttccggt cgcgaaagcc
     5041 atcctcgctc gcgggagctg ttaagctcgc taccgttagc cattgattat gaagtgctgt
     5101 ttagtgatgt accctgcgtc tggttacgca aagatcatcc ggcactgcat gaaacgtgga
     5161 atctggacac ctttttacgt tatccgcata tcagcatttg ctgggaacag agcgacacct
     5221 g
//
LOCUS       DAEEDS010000178         5165 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEEDS010000178 DAEEDS010000000
VERSION     DAEEDS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.181"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..222
                     /locus_tag="KLH32_003995"
     CDS             <1..222
                     /locus_tag="KLH32_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0140619.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="KLH32_003996"
     CDS             complement(394..723)
                     /locus_tag="KLH32_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBE0140620.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="KLH32_003997"
     CDS             complement(728..913)
                     /locus_tag="KLH32_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBE0140621.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="KLH32_003998"
     CDS             complement(910..3549)
                     /locus_tag="KLH32_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBE0140622.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="KLH32_003999"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="KLH32_003999"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE0140623.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5165
                     /gene="hslJ"
                     /locus_tag="KLH32_004000"
     CDS             4857..>5165
                     /gene="hslJ"
                     /locus_tag="KLH32_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBE0140624.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1306 a         1323 c         1205 g         1331 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattggccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaacctgca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg
     5161 ataac
//
LOCUS       DAEEDS010000179         5156 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEEDS010000179 DAEEDS010000000
VERSION     DAEEDS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.182"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            188..445
                     /locus_tag="KLH32_004001"
                     /pseudo
     CDS             188..445
                     /locus_tag="KLH32_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KLH32_004002"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KLH32_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBE0140625.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KLH32_004003"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KLH32_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBE0140626.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KLH32_004004"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KLH32_004004"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBE0140627.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="KLH32_004005"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="KLH32_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="KLH32_004006"
     CDS             complement(3603..4418)
                     /locus_tag="KLH32_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0140628.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5156
                     /locus_tag="KLH32_004007"
     CDS             4515..>5156
                     /locus_tag="KLH32_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0140629.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVH"
BASE COUNT         1376 a         1175 c         1226 g         1379 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gttcac
//
LOCUS       DAEEDS010000180         4996 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEEDS010000180 DAEEDS010000000
VERSION     DAEEDS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.183"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..249
                     /locus_tag="KLH32_004008"
     CDS             <1..249
                     /locus_tag="KLH32_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE0140630.1"
                     /translation="SLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAA
                     MVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(450..1307)
                     /gene="sitD"
                     /locus_tag="KLH32_004009"
     CDS             complement(450..1307)
                     /gene="sitD"
                     /locus_tag="KLH32_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBE0140631.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="KLH32_004010"
     CDS             complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="KLH32_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBE0140632.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="KLH32_004011"
     CDS             complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="KLH32_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBE0140633.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2985..3899)
                     /gene="sitA"
                     /locus_tag="KLH32_004012"
     CDS             complement(2985..3899)
                     /gene="sitA"
                     /locus_tag="KLH32_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBE0140634.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4361..>4996)
                     /locus_tag="KLH32_004013"
     CDS             complement(4361..>4996)
                     /locus_tag="KLH32_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0140635.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1327 a         1221 c         1195 g         1253 t
ORIGIN      
        1 tcactgggcc gcctgtcccc ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat
       61 atggcgtgga gcgatgccga taatcagcag gtgaaactgt cgatgccgga actggaagaa
      121 ctggcggcag caatggtgca ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag
      181 cgggagctga aggaagagct gaatagtctg aaggatttga attcggttag gaattttatc
      241 gtggaataac agaagctgcg gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa
      301 ctttcgatag tgcgagtatt gaatgatttc taggcgttat cgattttacg tattttttgc
      361 atgatagaat tcgtacctcc tcccaacgac cttccatggc tttccggact tctgtaggct
      421 ggcggacgat gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg
      481 gcgtttccct ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacacgacc
      541 gcgaataaga cgacaatcgt cggagctggt gcgctgtcga taaaaaagga cagccagaca
      601 ccagtcattg acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt
      661 tgtgtcagga gtactgcaat ggcgccaggc gcaatgagca gagaaataga gagaatgata
      721 cctactgctt tcagcgtcgc cacaatggtc agggaaacca tgcacagcag cccataatgc
      781 agccatctcg tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa
      841 aaatcccgcc attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg
      901 attatatcgc cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg
      961 tctggtttaa ttgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc
     1021 cccataactg tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg
     1081 cagaatactc cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggtaagc
     1141 acgatgccag gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg
     1201 accagaaaga ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg
     1261 tgactcataa aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca
     1321 gcgctttatg cgcacgccga cgatttgcca aaagtccatg ggtgggcgca aatacaaatg
     1381 ctaaaagaaa cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa
     1441 agtaactgac ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga
     1501 gcaggcgggg aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac
     1561 agatcaccag aaacgcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg
     1621 tgaagaagag gatttttaat ctgcccggat gtaatccaat agcacgggcg tgattctcgt
     1681 cgaaaaaggt caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc
     1741 caatgatagt gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga
     1801 tggtctgaat gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa
     1861 aggacgagaa gatcaggccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa
     1921 acagcatgct gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc
     1981 ccagcatata tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca
     2041 gcgaccagcc ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccattg
     2101 ccgagaccca catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca
     2161 tcttgcttcc ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt
     2221 aataatgctt tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag
     2281 ctccaggttc tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt
     2341 gaccatgacc gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag
     2401 catcgttttg ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt
     2461 cacatcaacg ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaattgc
     2521 tctcgccaga aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gacgatggcg
     2581 aaaatccacc atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt
     2641 ggctatacgc aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag
     2701 tacaggaaat gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg
     2761 tagcgcctgt cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa
     2821 tcccataatc gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc
     2881 aatagatcca cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca
     2941 ggtgacagta acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc
     3001 ccggctttaa ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgta
     3061 ggtaccgggc cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg
     3121 ccggtttcac gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact
     3181 gccgggatat gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct
     3241 tgttgatcgg cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga
     3301 taagaaaaag ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc
     3361 tgcttacgca ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt
     3421 tggtaggttt gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg
     3481 taaatcagag cattatctgg cgacatccat gcatggggat taggtttgcc ctcatagggt
     3541 ccttcggtga tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccattg
     3601 agatgctggt aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt
     3661 tgtgccccct gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca
     3721 ccaggcttgg ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatcc
     3781 gcgatgatgg taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg
     3841 atcgagcagg tgagggcgag gcaccccaag agcatggtta ctttttttat tgagtgcata
     3901 atttagtacc tatagtgagc aaaattagaa caaacatagc aacggctatg ttttatagca
     3961 taacctatac aaaatgaaaa taattatcat taacagcttt gaggcctaaa acagggatta
     4021 ggttgtttcc tgctggcgac atgatattga ggaaaaaagt agagcagcta ttaggctttg
     4081 aaatgacttt atgaggaaaa ttgttgatgg caaaaatggg gcaaaacgct gtataagggg
     4141 caaaaaaggg gcaaaaaaga gtgggttatc gtagcttatt gttgtcgctg ataatgttta
     4201 acatattgaa aaataaataa aatacttatg agtcagatag ttgtgatttt tgcccttact
     4261 tgttcaggtc gtattgttct ttctttgtaa tttgttgatt ttcttgcatt atttcagttc
     4321 tctggtacta aatggggcaa attgggggca aactttgcaa ctacgataac cgcgcattca
     4381 acatggctat ctgttcgtcg ttcatgtcat caatccacat accgtaaatt tcatacacca
     4441 tttgcgcagt ttcatgcccc atctggctgg ctataaatgc cgggttcgct cctgccgtca
     4501 acagccagca ggcaaaagta tgtcgcgtgt ggtacggatt acggcggcga ataccagcac
     4561 gttttactgc tgcattccat cttgtaccca aactgcttac cgagtaataa ggtttctgtt
     4621 tttcgttaca caccctgggc atgaaaacaa aatgcagttt ttgtttttca gttctgccgt
     4681 actcccgatg ataaaaagtg atttcgcttt tgcgatgatg cccggtcagt ttgtattgct
     4741 ccttcagtgc ttcaagagcc ggctgcagta atgttaccgt ccggatcccg gcatttgttt
     4801 ttgggggacc gaacatatca agtatcgtca ggtttcttct gacattcaca attcccttct
     4861 cgaaatccac atcctcccac gccagagctg ccagttcccc gtgacgaagc ccggagtaaa
     4921 cggcaaattt ccacaagttt tggctctgtc ctttttcact ttccattaat gcattgaatt
     4981 ctgttttaga taacgg
//
LOCUS       DAEEDS010000181         4990 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEEDS010000181 DAEEDS010000000
VERSION     DAEEDS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.184"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            291..851
                     /locus_tag="KLH32_004014"
     CDS             291..851
                     /locus_tag="KLH32_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HBE0140636.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            794..1183
                     /locus_tag="KLH32_004015"
                     /pseudo
     CDS             794..1183
                     /locus_tag="KLH32_004015"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1163..1720
                     /locus_tag="KLH32_004016"
                     /pseudo
     CDS             1163..1720
                     /locus_tag="KLH32_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1818..3427
                     /locus_tag="KLH32_004017"
                     /pseudo
     CDS             1818..3427
                     /locus_tag="KLH32_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3528..4019)
                     /locus_tag="KLH32_004018"
     CDS             complement(3528..4019)
                     /locus_tag="KLH32_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HBE0140637.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4024..4200)
                     /locus_tag="KLH32_004019"
     CDS             complement(4024..4200)
                     /locus_tag="KLH32_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0140638.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
     gene            complement(4589..>4990)
                     /locus_tag="KLH32_004020"
     CDS             complement(4589..>4990)
                     /locus_tag="KLH32_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140639.1"
                     /translation="SRAVIGWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT         1500 a          858 c         1000 g         1632 t
ORIGIN      
        1 cccaatgcct ttttcaaaat aaattaggat taaaataatt aaatcagtaa attccgatgc
       61 atgatttcgg atttttcgaa agtcctgatg aaaggctgcg ctacggcact cacggattta
      121 attgttattc ctaatgtatc tatgaattaa tgttttataa ggatttttta taaccattta
      181 taagtatttt caagcctggc ttgttgcaaa caatgtataa agcacttagg caataataat
      241 tacattcagc aactatcatc atcggtattg tttgtgggcg gaatttcata ttgacaacag
      301 tacagttctt atatctatta ataatagaaa gagatctaca acctacagat tggtgtagct
      361 ttatggaaaa agactatttg agaattagta gtactgtatt agtgagctta ttgtttgggc
      421 ttgctttggt tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac
      481 gatcaaccta tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt
      541 ttatgcagtt gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg
      601 cttttctgag ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg
      661 aggggcgttt gacaagcagt gttgtccaaa atgacattgc aatctattat ttgtttcgtc
      721 agatgagttt gtgcatatta atatttctgg cattgatgaa taaagttagt gaaaacacaa
      781 aacagcgcaa tttattttcc aaaaaaatga ctttgtgcat aagtttgttt ttgtttttgg
      841 ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt tacacattgc
      901 cgagttaacc aatgaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      961 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctatgatat
     1021 ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct ctctgttatt
     1081 tatgagcagg tatagcgtgt ccacttggta tattagtcgc actattgaag tagtcagtaa
     1141 gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag taacaaagaa
     1201 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
     1261 gacagttcaa tcagcatcag ccaaaaaaac gccttattgt gtcatgatta tggatatcga
     1321 ccacttcaaa gaagtcaacg acacctgagg gcatccggtt ggcgatcagg tgataaaaac
     1381 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgtcggcgg
     1441 cgaagagttt ggcgtcttgc tgacggacat cgatactgaa cgtgcgaaag ctttagcgga
     1501 aaggattcgg gaaaatgttg agcgtttaac tggcgataat cctgaatacg ctataccaca
     1561 aaaagtgacg attagtattg gcgctgttgt gactcaggaa aatgcgttaa acccgaatga
     1621 gatctatcga ctggctgata acgcactata cgaggcgaaa gagaccgggc gtaataaggt
     1681 ggttgtgagg gatgtggtga gtttttgtga gtcaccataa agcggcattt tagcgggtga
     1741 ttttggtaag cgctaaatag ctattcgcag acttgcgtaa caattttcag aaatatcata
     1801 gtactatatg atcttaaatg cttatcattg atcaatctgc tatcacccgc ttttactggc
     1861 aattgtacta taatttctta ggttgcatca ccctgatgcg tggggaaaca tgtatggtat
     1921 tcaacaaaaa aatgtttgta ctcattataa taccaggcat tctgggggtg ttactatcgt
     1981 ttgctatgtc aatatttcaa atgaatagag atacgacaat taccgccggt attctgttaa
     2041 aacagcttga taatgttacg caaattgtcc agcatacgac aaagcttacc tctattctgg
     2101 tgatgaagcc ctgcaaagac attcttgaac agttgattgc taacggagca ttgactccct
     2161 atgttcgaac aacgggtctt atagaaaata atttccaaat atgttcttca gttagcggtt
     2221 tcaaaaaaat gaatgttaac gatgtttacg gtacgagctt tcacaataaa aataaagaat
     2281 cacgtatagt atcaattagc ggtacaagtt ttgtgccagg taaaacagca atagtgttcc
     2341 ttatgccaat agggaatgat atgacagctt ttagtattgt agagtcacga tatatctatg
     2401 atctaatgga tgttcttgat gatgagaatg atgattcatt ttctcttcgt tttactgaag
     2461 ggcctgcaat tattagcggt gtaaataata atgacaggtt atatatgtta aaaagagatt
     2521 ttaattctgc gatcagtcag gctcgtctta cagttactac gccgatgata tcgttatatc
     2581 cctatatcat tagcaatgtg ctttatattt tgccgttatc tattattctc tcttttattc
     2641 tctatttctt atggcagcac tggatgtcac ggaaaatgtc gctggcagaa gaaattaaaa
     2701 aaggaatgtc atccggagaa ttttctgtgc attatcaacc agtgtgcgac acgacaacca
     2761 aagcgtgtct gggggttgaa gcgttaatgc gctggcaacg cgaagatggt aaaaatatat
     2821 ctccggtagt ttttatccgc gctgcagaag aggaaaattt aataataccg ttaactaaac
     2881 atctctttga actgattatt caggatgtac aaagctggaa agtgaaaaag ccgtttcacc
     2941 ttgggataaa tattgccgcg gaacatcttg cacatcctga ctttgttggt gatgttttac
     3001 atattaaaaa tgcaatatct gataaattta atattgtcct tgagattacc gaacgcaatc
     3061 tggttgaaga cactgatctt gcattacaga aaataaatga attaaggatc catggctgtg
     3121 aatttgcagt cgatgatttt ggcacgggat attgttccct tggccttctg caaaagttgt
     3181 ctgttgatta tcttaaaata gataaaagct ttatagacac acttactaca gcggaagacg
     3241 aaacgccagt tttagatatt attattaagc tgagtaatcg gctcaatttg ataacgattg
     3301 cagaaggtgt cagtactcct caccagcaga atatcttatt cataataacg tgacattagt
     3361 tcagggatac ttatatgaaa aaccaatgaa agcgacagag ttttatcagt ggtatgtaaa
     3421 cagataaata attaaaaata ttggaatggt tttgtattgt cttaaaatat aaacaaagca
     3481 atcagaatgt tattaactga tgacaaacgc caagatcaac aatgctgtta gtgagtaata
     3541 catatgaatc gtggtgtcct gcctgtagag aatatgtagt tataatcatt ttgacgcgta
     3601 acataataat taattgaagt gtctgctttt atgaagaaat cagaagtgat attttcaagg
     3661 atttcatcgc tgatgttatc atctttttgt attttttgga ttttaactgt ggtgagataa
     3721 acggtatcac ggaatttctt gtatgtaaaa ctcacatatc ggcggatgaa gcccattttt
     3781 tttccatcta ccgtgtatgc gcccgtgaga tacatgtttc ctgtgttatt ttcagaaatc
     3841 atatttaacg aaaaatattg atccattttt gtatgatcgg tgaaattaat aacatgggca
     3901 ttacatgata tgttttgctc tgctggttca ttggaatgat gaaacgcaat atggctcaga
     3961 attgctgcaa taaaagcaca taaaattatt cccgaaaaca gaaaatatct ttgagtcata
     4021 tgattactcc gctttataat agagagagtt gcaattattt ggtgattcag agtttaacat
     4081 ttgactacag gtgaaagcat aaaacccgct catgctatca tttgttgcaa tgtaaatgta
     4141 aggatgtatt ttgcagttga cttccatttt ctgcaatttt tcaataattg gtgatagcat
     4201 gtctacggaa ttaaagtaat tattatcata gaaataactg catccttcct gagtagggtg
     4261 ttgagcataa tttgagaaag catctatttt ttttatagtg tgagatttgc tttgaaaagc
     4321 catatcgaaa aaataagaag acatataaac tatcccccat gccgcaaaaa aaaactataa
     4381 gtaatgtatt ctttgtggta aatatacctt ttgcaacgta atttttattt atattttcag
     4441 tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta ttgtcgagaa
     4501 cagttatgtt gatttcttca ttgattttga agcctttccg tggcaccgtt ctgataactg
     4561 atcctaccca cgtaatatgg acacaggcct aagcgaggtt ctggttttca aattgttccg
     4621 gactgaggcc gccacaccaa ctgtgccgcc gccaccgatt gtaatcacat tcgatataat
     4681 taaacaccgt tgcccgcatt atttcccggc tgataaagtg ttctccatgg atacattcca
     4741 ctttcagcga atgaaagaag ctttccacgc aggcattatc gtagcagcaa ccttttgcgc
     4801 tcatacttcc acgcagatta tgccgcttca gttgcgcctg ataatctgct gaacagtact
     4861 ggcctccacg gtccgtgtga acgataacgt tccggggcct cttacgccgc cacagcgcca
     4921 tctgcagggc atcgcaggcc agttgcgccg tcatgcgtgg cgacattgac cagccaataa
     4981 cggcacgtga
//
LOCUS       DAEEDS010000182         4973 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEEDS010000182 DAEEDS010000000
VERSION     DAEEDS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.185"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="KLH32_004021"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="KLH32_004021"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBE0140640.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="KLH32_004022"
     CDS             1020..1703
                     /locus_tag="KLH32_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBE0140641.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="KLH32_004023"
     CDS             1700..2161
                     /locus_tag="KLH32_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBE0140642.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="KLH32_004024"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="KLH32_004024"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE0140643.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="KLH32_004025"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="KLH32_004025"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBE0140644.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KLH32_004026"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KLH32_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBE0140645.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KLH32_004027"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KLH32_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBE0140646.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1175 a         1303 c         1240 g         1255 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAEEDS010000183         4902 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEEDS010000183 DAEEDS010000000
VERSION     DAEEDS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.186"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..666)
                     /locus_tag="KLH32_004028"
                     /pseudo
     CDS             complement(1..666)
                     /locus_tag="KLH32_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(269..385)
                     /locus_tag="KLH32_004028"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            720..1161
                     /gene="tnpA"
                     /locus_tag="KLH32_004029"
                     /pseudo
     CDS             720..1161
                     /gene="tnpA"
                     /locus_tag="KLH32_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1279..2223)
                     /gene="hypT"
                     /locus_tag="KLH32_004030"
                     /gene_synonym="qseD"
     CDS             complement(1279..2223)
                     /gene="hypT"
                     /locus_tag="KLH32_004030"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBE0140647.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            2568..3287
                     /locus_tag="KLH32_004031"
     CDS             2568..3287
                     /locus_tag="KLH32_004031"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBE0140648.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            3353..4651
                     /locus_tag="KLH32_004032"
     CDS             3353..4651
                     /locus_tag="KLH32_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBE0140649.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1205 a         1118 c         1206 g         1373 t
ORIGIN      
        1 caacggcttt ccggctgatg gtcgtacgga cctttttacc ccggagaaca ccggcaagtc
       61 ccataaccgc catgagacgt gccacagtgc atctggccac tctgatacct tcccgtaaca
      121 actgacgcca gactttacgc acaccgtata ccttgtgatt ttcatcgtat acgcgctgta
      181 tctctttctt cagccagtca tcgcgctgcg cacgggcact gcgtttatcc ggatgatgtc
      241 gctgttgctg acagtggtaa tacgttgacg gggcaatatg cagttcgctg catagcggtc
      301 cgaccccgta ctgctcacgc agcttatcca gcagtggcat cattttttcc agaggcggtc
      361 gaactctgcc ttcgcaaaat aagcggaagc ctggcgaagg atatcgttac tgcggcgcag
      421 ttcacgattt tcacgttcca gctctttcag acgctgacgt tcagcggtgg tgagccctcc
      481 atcaccgccc ccggtatccc gctcatgctg gcgaacccag acacgcagag tctccggcgt
      541 acagccaatc tttggagcaa tggaacaaat tgtcgcccat tgtgagtcat attcgctctg
      601 actttccaga accatacgga ctgcccgttg acggacttcg ggggaaaaac gagtattttt
      661 agtcatcctg tttacctctt tctcaggaag tttagtctcc aggattcccg gggcggttca
      721 tagcgcacac ccgatggaac tgtaaatatc acatagtttt ttgcgccaaa ataccgaaga
      781 caggtgttct acagagagaa gcgtagagca acaggcagta ttttgagaaa gctgtgtgag
      841 tggaaaagcg tacggattct ggaagctgaa tgctgtgcag atcatatcca tatgcttgtg
      901 gagatcccgc ccaaaatgag cgtatccggc tttatgggat atctgaaagg gaaaagcagt
      961 ctgatgcttt acgagcagtt tggtgatttg aaattcaaat acaggaacag ggagttctgg
     1021 tgcagggggg actacgtcga tacagtgggt aagaacacgg cgaagataca ggattacata
     1081 aagcaccagc ttgaagagga taaaatggga gagcagttat cgattccctg tccgggcagc
     1141 ccgtttacgg accgtaagta acgaagttgg atgcaaatgt cagatcgtgt gcgcctgtta
     1201 gggcgcggct ggtaagagag ccttataggc gcatttgaaa aacctccggc tatgccggag
     1261 gatatttatt gcacgtcgtc aattgcggcg tgctggctct tccattgata tctgctggat
     1321 taaatcgtct ggcatatgtt cctgtaaaat acgccagagg ttctcggcgg ttctattcag
     1381 tcgggtgttc attcggtaaa tataagcctg aatggggact gtcaatttag cagcatcaag
     1441 gcatatcaga cgtttggtct gtagttcatc gacgatcgac cagatcggta accaggcaat
     1501 gccgtatccg ttgagcgcca tattctttaa taactcgctc atcgaagaga taaatatcgt
     1561 gcgcccggta atacctccaa cctctgccag atgacgattg accaacctgc ccatataaga
     1621 tgtggcggta tagttgagca aaggaacctg gggctgagtg atatcgaata cagggtgccc
     1681 ctgtgcgtcg gcggcacata ccgggtacaa ctcagattca aacagtttca ggcagcaaaa
     1741 aggcgattgc ataagatctt catcgcgaaa cgaaataata aagtcactct tgccttcgcg
     1801 taacgtattt acggcctgga caacatcaat ggcttcaaca tgatagacaa attctccgcc
     1861 ataggcggta agtgaatgca ccagtttggg taatacgctc agggatagtg agtgcgcggc
     1921 tgcaatttta atgttgggaa cgccgagtaa actctggccg tttaactccc ccaaattgca
     1981 ttcaagctgc tgtaataagc tgcgagtctg ggaatgaaag agcttgccct cttcggttaa
     2041 ttgtaaaggg gtggtcgtgc gatcgaataa aacaacgccg atcgctgcct ccagcgcctt
     2101 aattcgtcgg ctgaatgcgg gttgcgaaag attacgctct tttgccgcct gagaaaaatg
     2161 gcgacaggct tccagtgtca ggaaatcgta taaccatttt gtctcgatat tcttcattac
     2221 catggcacct gtattttcaa tccaaagaca taataaaaca tcccggcaat aaaaataatg
     2281 ttctcaatta gcacaacctg gcctttatca ttcgttttgt gcataacccc ttcatttccg
     2341 gtattgacgt tgatatcaca tttcgccaaa aaaacgaaat ttttccgtca gataaccaga
     2401 atacatcatc actatggtga ataagcggaa tgttccatag ctgcatcatg cgtgtcttgc
     2461 atcgccttgt acaaccttgc atttctcatc gcccatttcg actgttaaaa aagagagcag
     2521 cagttattta ccgcgtattg tcattaacgt tttgagaggc aggtattgtg aaaggtttaa
     2581 ttggtgtact cggtggaatg gggccggcag ctaccgtcga cctgtttaat aaatttgtaa
     2641 cttttaccgc tgcacaatgt gatcaggaac atattccttt aattatttct tcgatcccgg
     2701 atattcctga ccgtaccgat gcattaatgc atcatgggcg ttcaccatta cccgttatgc
     2761 gtgattatat gcataagctg gaagatgccg gggcagaatg tattgttatt ccctgtaata
     2821 ccgcgcatta ctggttcaaa gaattaaaag atgcttgtca tattgatata ttaagtatcg
     2881 tagaaactac gatcaatgaa gttcgcgcct gtggtaaaac ccgaattggc ttgttagcca
     2941 ccaatgccac gctatatatg ggtttatacc aaaaaggaat tgagtcgctg ggatttacct
     3001 gtgtcagccc cgatgccgac ggtcagaaaa aagtgatgga aagtatttat tccttgaaag
     3061 ctggcaatat agcgcacgca caaaagttga tgaatgagca ggctgaaata ttattttccc
     3121 gcggtgcaga aatccttgtt cttggttgta cagaaattcc ggttattctg gcgcaagccg
     3181 ttaaagagca gccttcccgc tatattgact caacggcgtc actcgttcgt gccggaataa
     3241 aatggtacga aaatcgtgtc ggtaaacatt atcttttaac ccaataatca tttaaatcgc
     3301 agccagaaag ttattcgctt ttaactgaat tatatttata acggagaaca ttatggtttg
     3361 gctggaaatt atcgtagtac ttggtgcaat attttttggt attcgccagg ggggaatcgg
     3421 tattggttta tgtggcgggc ttgggcttgc cattctgact ctgggatttg gtctgcctat
     3481 ggggtcgccg ccagtcgacg tgatcctgat tattatgacc gttgttgtgg cggcctcagc
     3541 cttacaggcc gcaggcggaa tggattatct ggtacgcgtc gctggtaatt ttatgcggcg
     3601 taacccgaaa tatatcaaca taatcgcgcc gattatcacc tggtctatga ccattatggc
     3661 cggtactggg tttattgttt tttccacgct gccggtgatt gctgaagtgg cgaaagagtc
     3721 cggtatccgt ccttcccgca cgcttgccgg ttcggttgtc gcttcgcagg ttgcgatttc
     3781 cggttcgccg atcagcgccg caatggcggc tatgctcact attatggagg taaacggtgt
     3841 cagttttatt caagtgatgt cggtatgtct tcccacctct tttgctgccg cgatggtcgc
     3901 cgcttttatt gcttcccgac agggatgtga gttgcaggat gacgaggtct accttgagcg
     3961 cctgcaaaaa ggactggtgc aaaaatacga gaataatagc agcatcaagc caggtgcgac
     4021 gctctccgtc ggattgttcc tgctggcgac ggtcgctatt gttattcttg ctgccttccc
     4081 ccaactgcgt ccgggttttg atatcggtaa accgatggaa acacgcgata tcattattat
     4141 ctgtatgctc tctgccgcct gcctgatggt ggtgctgtgt aagacggcga ccgatgccat
     4201 tattctcacc tccacattcc gtgcggggat gagttcgctg gcggtaattc tcggcattgt
     4261 aaccttaggc accactttta ttgatgcgca tttaactgag attaaagata ttgctggtga
     4321 tattttgcag gcgtatccga tgttgttggc gctggttctt tttggcactt gcgcgctgct
     4381 ctactcgcaa ggtgcgacca cgccgttaat tatcccgctt gccgtcgcgc ttgacgtgcc
     4441 cacctgggca attctggcgt cctatgtggc cgtcaccgga gtatttgtac tgccaacgta
     4501 tccaacatca ctggctgcca tggaatttga tactaccggc acaactcgtg taggcaaata
     4561 tgtgctgaat catccattta tgctgcctgg gttgggggga attattgctg gagtggcatt
     4621 aggctttgtg attgcgccaa tgattattta gcactatcaa acatcagtat cgatggttta
     4681 tacacaataa agacagatat ggcggaatat agtctgttgt aagcgccaac aaagaatagg
     4741 ggaattccgc catgtctgaa catcttcaca atatggtatt aagtgttgtc gtttaatgat
     4801 ttatttcttg gttatgttgt gtattagctg catctatcgc tttttgatac ctgaggtgta
     4861 ctggcaatag cggacactac catttgttct ttttttaagc ag
//
LOCUS       DAEEDS010000184         4878 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEEDS010000184 DAEEDS010000000
VERSION     DAEEDS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.187"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..128
                     /locus_tag="KLH32_004033"
     CDS             <1..128
                     /locus_tag="KLH32_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBE0140650.1"
                     /translation="TAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            255..1115
                     /locus_tag="KLH32_004034"
     CDS             255..1115
                     /locus_tag="KLH32_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBE0140651.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1325..1729
                     /locus_tag="KLH32_004035"
                     /pseudo
     CDS             1325..1729
                     /locus_tag="KLH32_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1836..2672)
                     /locus_tag="KLH32_004036"
     CDS             complement(1836..2672)
                     /locus_tag="KLH32_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBE0140652.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2672..3475)
                     /locus_tag="KLH32_004037"
                     /note="aph(3'')-Ib"
     CDS             complement(2672..3475)
                     /locus_tag="KLH32_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBE0140653.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(3536..4351)
                     /gene="sul2"
                     /locus_tag="KLH32_004038"
     CDS             complement(3536..4351)
                     /gene="sul2"
                     /locus_tag="KLH32_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBE0140654.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4681..4857
                     /locus_tag="KLH32_004039"
     CDS             4681..4857
                     /locus_tag="KLH32_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBE0140655.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
BASE COUNT         1201 a         1276 c         1315 g         1086 t
ORIGIN      
        1 agacggctta cgccaccatc aaaggtattg aggtgatgcg tgcactacgc aaaggccagg
       61 cctcagcatt ttattatggt gatcccctgg gcgaaatgcg cctggtaagc agagtttttg
      121 aaatgtaagg cctttgaata agacaaaagg ctgcctcatc gctaactttg caacagtgcc
      181 tacattcaaa tatgtatccg ctcatgagac aataaccctg gtaaatgctt caataatatt
      241 gaaaaaggaa gagtatgagt attcaacatt ttcgtgtcgc ccttattccc ttttttgcgg
      301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      481 gtgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
      541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgct gccaacttac
      661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      781 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
      841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
      901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
      961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1261 cctaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg
     1321 gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca
     1381 gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt
     1441 cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga
     1501 agaatttctc caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg
     1561 ccacgccgag cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg
     1621 ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc
     1681 catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg
     1741 cgggaagggg cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga
     1801 tgtgttccca ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc
     1861 ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct
     1921 gcggaaaggc acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc
     1981 gcacgagaga atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct
     2041 ctgtcagccg gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg
     2101 acgggatcta tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg
     2161 ccatgtagcc cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg
     2221 tggacgtagt cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct
     2281 gcaaagcgat cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac
     2341 agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg
     2401 atgtgagaga gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg
     2461 agcaaccgga ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca
     2521 tcagcaatgt cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat
     2581 gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc
     2641 caatgagcag gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca
     2701 gatagaaggc aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag
     2761 cgaaggcgcg ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga
     2821 gtgccaaatc ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc
     2881 attgaagagt tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa
     2941 ccatatcggt gcgctcttgg tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa
     3001 gatcgagctg cggcgtactc ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc
     3061 gggacaccac atcaacggcg cgtccgaaca ttcgcgacag cctgcgctca aacggacatt
     3121 gatcaaccga taggctgtga acagcgccaa gttgctgccc cattgacggc cacgctttga
     3181 gcaaatccgc tccagacaga tcagccgccg gtactcccgg aattgccgtt atcaccaagc
     3241 atgcaccctc ctgttcctcc tgccagttga tgacctcggg gcaagccaca cctcgacctt
     3301 tgagccaaat gaggcggtca cgctctccag cgagctcacc gcggcgggaa gcaggtgcga
     3361 ttttcgcgaa ggcatgcccg tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc
     3421 tgacaggcaa ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag
     3481 gttccttcag ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac
     3541 gaattcttgc ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg
     3601 gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagcgagtg
     3661 tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg
     3721 aaacagacag aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca
     3781 ccgagagcga ggtttcggga gcagccccca gaaaaaaccc catgccggga tcaaggacaa
     3841 ggcggttgcg tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg
     3901 caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca
     3961 ccgaatgcat aacgacgagt ttggcagatg atttcgccaa ttgcggatag aacgcagcgt
     4021 ctggaaaacc gcgaatatca ttgagatagg ccacaccacg cgacaaggca taggcttgcg
     4081 tcgcgggttg ataactgtcg agcgagacgg gaatgccatc tgccttgagc gcgtccagca
     4141 ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg
     4201 atgccggacc gaggtcgatc acatctgccc cctcggccat cagcttacgc gcctgcgcaa
     4261 tggctgcgtc tggcgccaga taccggcctc catcggagaa actgtccgag gttatgttga
     4321 cgatgccgaa aatgatgagc gatttattca tgggggcttc tataataata ataatcgagc
     4381 atgagtctca tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg
     4441 gtgctccatt agctgcagga gcagaatagc atacatctgg aagcaaagcc aggaaagcgg
     4501 cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt taagcctttt
     4561 ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca ttgaatataa
     4621 aagataaaaa tgtcttgttt acaatagagt gggtatcgga gacgaagaag gacaaggtac
     4681 atgctgaccg ataccaagct gcgcaatctc aagcccaggg acaaactcta caaagtgaat
     4741 gaccgggaag gtctctatgt gggggtcgcc tcagaaaacg gaatctatgg tcactcccgt
     4801 ttttgcaaca ccgattttga cgacaagttg gcttgcttga atctatccgg cgtctgaatg
     4861 ggattttatt cccgcgcc
//
LOCUS       DAEEDS010000185         4872 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEEDS010000185 DAEEDS010000000
VERSION     DAEEDS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.188"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..528
                     /locus_tag="KLH32_004040"
     CDS             <1..528
                     /locus_tag="KLH32_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBE0140656.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            725..2164
                     /gene="aldA"
                     /locus_tag="KLH32_004041"
     CDS             725..2164
                     /gene="aldA"
                     /locus_tag="KLH32_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBE0140657.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2206..3207)
                     /gene="gap"
                     /locus_tag="KLH32_004042"
     CDS             complement(2206..3207)
                     /gene="gap"
                     /locus_tag="KLH32_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE0140658.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3396..3926
                     /gene="cybB"
                     /locus_tag="KLH32_004043"
     CDS             3396..3926
                     /gene="cybB"
                     /locus_tag="KLH32_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBE0140659.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4171..4344
                     /locus_tag="KLH32_004044"
     CDS             4171..4344
                     /locus_tag="KLH32_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140660.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4416..4565)
                     /gene="hokB"
                     /locus_tag="KLH32_004045"
     CDS             complement(4416..4565)
                     /gene="hokB"
                     /locus_tag="KLH32_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBE0140661.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4666..4806)
                     /locus_tag="KLH32_004046"
                     /pseudo
     CDS             complement(4666..4806)
                     /locus_tag="KLH32_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1121 c         1208 g         1296 t
ORIGIN      
        1 cgcaccactg gcagagcaga agcgaccatc ctggtggata tcgctcatca gttctggcat
       61 attccgcatg aaaaaatctg gattgaagac cagtcaacaa actgcggtga aaacgcacgc
      121 tttagcatca cgctattgaa tcaggccgta gaacgagttc atacggctat cgttgtacag
      181 gaccccacca tgcagcggcg cacgatggca acgttccgcc gtatgaccgg ggacaatccc
      241 gatgcaccac gctggttaag ttatcccgga ttcgttcctc agttaggaaa taacgcagac
      301 agtgtaatct ttattaatca gttacaagga ttatggccgg ttgagcgtta tctctcacta
      361 ctcactggcg agctgccgcg tttacacgat gatagcgatg gctacggtcc ccgcgggcga
      421 gattttatcg ttcacgttga ttttccggca gaagtcatcc atgcatggca aacgctgaaa
      481 catgatgcgg tgctcatcga ggcgatggaa agtcgctcgt tacgttaaaa attgcccgtt
      541 tgtgaaccac ttgtttgcaa acgggcatga ctcctgactt ttatttctgc cttttattcc
      601 ttttacactt gtttttatga agccctccac agacttgtcc tttcccgatg ccgtctctct
      661 gatgattgat gttaattaac aatatattca ccgaaaacaa acatataaat cacaagagtc
      721 gcccatgtca gtacccgttc aacatcctat gtatatcgat ggacagtttg ttacctggcg
      781 tggagacgca tggattgatg tggtaaaccc tgctacagag gctgtcattt cccgcatacc
      841 cgatggtcag gccgaggatg cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga
      901 atgggaagcg ttgcctgcta ttgaacgcgc cagttggttg tgcaaaatct ccgccgggat
      961 ccgcgaacgc gccagtgaaa tcagtgcgct gattgttgaa gaagggggca agatccagca
     1021 gctagctgaa gtcgaagtgg cttttactgc cgactatatc gattacatgg cggagtgggc
     1081 acggcgttac gagggcgaga ttattcaaag cgatcgtccg ggagaaaata ttcttctgtt
     1141 taaacgtgcg cttggtgtga ctaccggcat tctgccgtgg aactttccgt tcttcctcat
     1201 tgcccgcaaa atggctcccg ctcttttgac cggtaatacc atcgtcatta aacccagtga
     1261 atttacgcca aacaatgcga ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg
     1321 cggcgtgttt aaccttgtac tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa
     1381 cccaaaggtc gcaatggtca gtatgacagg cagcgtctct gcaggtgaga agatcatggc
     1441 gactgcggcg aaaaacatca ccaaagtgtg tctggaattg ggtggtaaag caccggctat
     1501 cgtaatggac gatgccgatc ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat
     1561 taatagtggg caagtgtgta actgtgcaga acgtgtttat gtacagaaag gcatttatga
     1621 tcagttcgtc aatctgctgg gtgaagcgtt gcaggcggtt caatttggta accccgctga
     1681 acgcaacgac attgcgatgg ggccgttgat taacgccgcg gcgctggaaa gggttgagca
     1741 aaaagtggcg cgcgcagtag aagaaggggc gagagtggcg ttgggtggca aagcggtaga
     1801 ggggaaagga tattattatc cgccgacatt gctgctggat gttcgccagg aaatgtcgat
     1861 tatgcatgag gaaacctttg gcccggtgct gccagttgtc gcatttgaca cgctggaaga
     1921 tgctatctca atggctaatg acagtgatta cggcctgacc tcatcaatct atacccaaaa
     1981 tctgaacgtc gcgatgaaag ccattaaagg gctgaagttt ggtgaaactt acatcaaccg
     2041 tgaaaacttc gaagctatgc aaggtttcca cgccggatgg cgtaaatccg gtattggcgg
     2101 cgcagatggt aaacatggcc tgcatgaata tctgcagacc caggtggttt atttacagtc
     2161 ttaatgagtg aaagaggcgg aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa
     2221 tttttcgagg gtgcgaataa gctgcgtgac gaagccatat tcgttatcgt accaggcgac
     2281 cgttttcacc agttgtaaat cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac
     2341 tgaaccgaaa tggctgccaa tgatatcgga agagactatt tcttcatcgg tataaccaaa
     2401 tgactcgtta ttggtggttg cttgtttaag tgcgttattc acctcttcgg cagtcacttt
     2461 ttttccgaga atggacacca gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc
     2521 atgacctttc agtttgccgc tcagttccgg gatcaccaga ccaatggctt ttgccgcccc
     2581 cgtagtgtgg ggaatgatat tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg
     2641 cgggccatcc accagtgact gggtgccagt ataggcatga atggtcgtca tcgtgccgac
     2701 ttctatcccg aaactgtcat gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca
     2761 tgacgccacg gaaacaatgg tgtcgttgcc atccagagtg tcgtcattaa cgttataaac
     2821 gatggttttc atttcaccgg caggagcgga aatcaacacc ttcttcgcac cagcatcaag
     2881 atgcgcctgc gatttatcgg cggaggtata aaagccagta cattcgacaa tgatttctgc
     2941 ccctttcgct ttccacggaa tatttttagc ctctttttcg gcgtaaaccg cgatactttt
     3001 cccatcaacg ataagtgaat cttccgtaaa atcaacgctc caggggaacg gaccatagtt
     3061 tgaatcatgt ttcagcagat aggcgagaat ttttggggaa gtaagatcat taatagcgac
     3121 aacgtctatg ttgcttttga cttcaagtaa acgacgcaac accagtcgac cgatacgacc
     3181 aaaaccgtta ataccaactt tactcatggt tttctcctgt caggaacgtt cggatgaaaa
     3241 ttgatccttt ccaggcttag accaggattg tgggatgggc aatctccatt cttacagtga
     3301 aacgtaacgt actgaaaacg ggtgaacaat atttaatgaa attttgagaa aagcccgtta
     3361 tgttaacgga aaattatgtt aaagcaggaa atgttatgga aaataaatac tcaaggttac
     3421 aaatcagcat tcactggctg gtctttttgc tggttatcgc agcgtattgc gcaatggagt
     3481 ttcgtggttt cttcccacgt agcgatcggc cgctcatcaa catgattcat gtttcctgtg
     3541 gcatctcaat tctcgtgctg atggtggttc gtcttttgtt aagactgaaa tacccaaccc
     3601 cgccgattat acctaagccg aaaccgatga tgacgggact ggcgcatttg ggacatttgg
     3661 tgatttatct cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccggg
     3721 gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac
     3781 gggttgatag cttaaagtcg tggcatgaga cgctggcgaa tctgggatat tttgtcatcg
     3841 ggttgcacgc tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac
     3901 gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg
     3961 acccgtggta caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt
     4021 atgacaaata aaaccataat ccttgcccat acgtccatct ggcttatttt taatcaattc
     4081 acccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcgc
     4141 acataagatt cgataaacca tggaggttat atgaaaaaac tggcacttat tttgtttatg
     4201 ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg
     4261 gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgca
     4321 tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct
     4381 agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca ggccatgagc
     4441 gcagcaacct ccttatcacc gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg
     4501 agtgtgaatg ccagaatcgt aatgcagata atgagcagac acaccaccag agggttgcgc
     4561 ttcatagcct ttctccttgc cggatggcgg gtaagaggct aacaatctga attgcttggt
     4621 tcattcgttg gcctcggttt gtactgaccc caaaaagttg gacagttaaa cacgaggcat
     4681 ataggtctga ttccgatatt catttggagt cagacctttt aatttcaggc taattcttct
     4741 gctgttgtag tattcaatat attccgtaac agcatccttc agttcgctta tattactgaa
     4801 ctcattagac tggccccctg aatctccaga caaccagtat cacttattta agtgatagtc
     4861 ttaatactag tt
//
LOCUS       DAEEDS010000186         4869 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEEDS010000186 DAEEDS010000000
VERSION     DAEEDS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.189"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..970
                     /locus_tag="KLH32_004047"
     CDS             <1..970
                     /locus_tag="KLH32_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE0140662.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1551..2774
                     /locus_tag="KLH32_004048"
     CDS             1551..2774
                     /locus_tag="KLH32_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140663.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3021..3587
                     /gene="slp"
                     /locus_tag="KLH32_004049"
     CDS             3021..3587
                     /gene="slp"
                     /locus_tag="KLH32_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBE0140664.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3743..4273
                     /locus_tag="KLH32_004050"
     CDS             3743..4273
                     /locus_tag="KLH32_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0140665.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4315..4869)
                     /locus_tag="KLH32_004051"
                     /pseudo
     CDS             complement(4315..4869)
                     /locus_tag="KLH32_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1557 a         1000 c          905 g         1407 t
ORIGIN      
        1 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
       61 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
      121 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
      181 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt ttggtccgac
      241 tctcactttt ttatttgttt caccattact taatcccatt atcgtcggac taatgtggat
      301 gacctttggt tggaaagtca ccttgttgta cgcgattatc gcagccggcg tctccgttct
      361 tgccagtatt atcctggatt ctctggggtt tgaacgtcat atcattgcca gtaaaagctc
      421 atcagcaaat tgttgtgctc cagcaaaaac ttcgccggga acgacatata cgcccataaa
      481 agtgagttgt tgtagtccag cggctaaagc cattgagaac cccgtagtta cctgttgcag
      541 taccaaagct gtagtaagta ttaatcccat aaaactagcc accaaagatg cgttgcaaca
      601 gtttaaagat gtactgccat atcttttgtt aggagtatta ataggatctt ttatttatgg
      661 ctttattccc tcagcgtgga ttgccgctca tgcaggagca gataatccct tcgccatccc
      721 attaagcgcc gttgtcggta ttccgctgta tatccgggct gaggcggtta tccctctggc
      781 atctgttctg atgacaaaag gaatgggtct gggagcatta atggcattaa tcatcggcag
      841 tgccggcgca agcctgacgg aagtgatatt acttaaatca atgttcagaa tgccgatgat
      901 agctgcattc ctgacggtta tattaggtat ggctatcttg atgggctatt tgactcaatt
      961 gctattttaa atagatgcca gcagtaaact ataagatgca tagttagtgc catatccttc
     1021 taagtagaaa atcatagaca ggataatcct taaaaatcac aaatgattta actaatgcat
     1081 tgagaatgat gacggctcgg tcaacaagtc atcaacagac aataaccacg taatcatgct
     1141 tctctgacat tttatcggag aagcaatgac tctttgtctc agtaccctcc tcctttttaa
     1201 aaggcagatt tcagtcgcta atacaacaca tttttaaata cactttaacc tggttcatta
     1261 ttacacatct aaaacatcac aaaaatatta cttcacacac tatcggagaa cataataact
     1321 ttttaaaatg agcgtttttt gatagttgtt aacgttttcc tttacgatac tatcactgcc
     1381 ctttttttat acacaaatat taccgggtgg aaatgaaagg gaatttaaag gtaattatat
     1441 caggaagata taacgcatta tatttattgt gtgtagaaga aaatccattc ctgttctaat
     1501 tatccaattt aaaaacatta gcataaaaca gatgatgaat aaggaatgtt atgtcaattg
     1561 actttacccc aggtataata aatacatatc acggcgatat ttataactgc acaacaaata
     1621 ccgataatgt taaaacgcca gacacaccga agtggccttg tgataattgg gaagaacaac
     1681 aacccattaa ttccacattc tctggagaag gatacaaccc tgaacaattt gatttagcgc
     1741 aacaacaact ccaacaaatt aatgcatgcc acacgaacac cacatataca aatgcagact
     1801 actcaaaagt tgtggttcaa cttgtaagtc ttattaataa cattgaaacg ataagttcga
     1861 caccacttac gcaacagaca caatcaatac ttaatcagat tcataacatt agatacgaaa
     1921 aggataagga ttctgtatgt caaatcattg ttgtcgttaa tccagaaccc aacaatccaa
     1981 tcataactaa aattctcgta gaagaaggaa tacccgaaag attcagtgtc caaaccgttt
     2041 cttccgataa taaaaatttt gctgggcaac gagctgactt accaactgat ataagagaca
     2101 ttcaaagctt gtacttaaaa atggcgaagc tgtatattga gcacagtaaa aatgaaaata
     2161 ggatggaagc ccttgctgga tgcgacttta ttgatttcaa catgacaggg caagatatgt
     2221 ccaagctagt cctaacctta tcaaaatttt attttgaaga tctacttaat ataaatttca
     2281 ctgacgcaaa tttatttaac actatttttt cacacaagga aaatcccatc cccaaactac
     2341 ataaatatga acaacatctt gacaaacaaa tcaatggttt gttttctaca ttgttaacta
     2401 ttaatgacaa ctctttacgc gcgaaagcgg aaattgcttc gagaataatt gatttcttag
     2461 aggcaaaggt agtgaatctt tcatttgatg acatattaaa atataagcaa gagttcaaaa
     2521 aaatatgtta taaacaactt caagagttta caacaccctc tctatacaat aaaattcaaa
     2581 aatgggcaac aatgtctaaa aatgaattca tagcatttca ttacgagaca cttcaacccg
     2641 aaaagatttc ataccccttt tatttaaaga gagatctgcc taatgaaaaa gatataaatt
     2701 atggggtgga aatagaaata ccatctggca aaagaattcg cttatcaaat cattatcaga
     2761 atataatacc ataatattga ttattagcac tttataatca ttgaataaaa atagatttta
     2821 tgtaatttta aaacaaaggg ttatattatt gggcgatgga tactcatacc acgccccaaa
     2881 ataacggaat tgaactaaac agaagggata taaacatcag acaggtttac gttactatca
     2941 ggcatatcac ctcagaatca gatgaaaact ataaagaaat atctattatg gttttaatat
     3001 ttgttgataa ggatagtaac atgaacatga caaaaggtgc actcatcctc agcctttcat
     3061 ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa aggcaataac caacctgata
     3121 ttcaaaaaag ttttgttgct gttcataacc agccggggtt atatgttggt caacaagcgc
     3181 gctttggtgg gaaggttatc aacgttatca atggcaaaac ggatacgttg ttagaaatcg
     3241 ctgtattacc gttggatagc tatgcgaagc ctgatattga agccaactat cagggccgac
     3301 tgctcgccag acaaagcggc ttccttgatc cagtgaacta tcgtaatcac tttgttacca
     3361 tcctcggtac cattcagggt gaacaaccag gctttatcaa taaagtcccg tataacttcc
     3421 tggaagtgaa tatgcagggc atccaggtgt ggcatttgag agaagtggtt aataccacct
     3481 ataacctgtg ggattacggc tatggtgcat tctggccgga accgggctgg ggtgcgcctt
     3541 actacaccaa tgcggtgagt caggtaacac ctgagctggt caaataacac caccgaaaga
     3601 tgcagatgct ttctcagcat ctgcatcatg tattacataa aattaatata cagtaagcta
     3661 actattatta ttataagccc tgtcctgtta attacctttg gcaaactgat tataaagtta
     3721 atgtccgcac caggagtcgg ttatgtttct tataattacc agggatacga tgttcttcac
     3781 cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat atacagaacg aagaagagat
     3841 cgacgtaatg ttgcatcaga atgccttcgt cattattgat acattaatga ataatgtatt
     3901 tcattctaat tttctcactc aaattgaacg attaaaacct gtccatgtca ttgttttctc
     3961 cccctttaat attaaacgct gtctggggaa agtaccggtg acctttgttc cgcggactat
     4021 cactatcatt gattttgtcg cactcatcaa tggcagttac tgctctgtgc ctgaagcgaa
     4081 tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc attgcgaatc aaatgacaac
     4141 tgaagatatt ctggagaaac tgaaaatatc gctaaaaacg ttctactgcc ataaacacaa
     4201 tatcatgatg atcctcaatc ttaagcggat caatgagctg gtacgccatc agcatattga
     4261 ttatctggtg tgaatttcag gcttacggtg agtctggcta cgctgccaca cagattagct
     4321 aattgaaacg cctttcaccc ctgccatacc ttttaataat cgcaacaggt cttctatcaa
     4381 cgttgtggca tgaagctgga tatcaatggc gaccacttca tgatcttcat tttcctgcaa
     4441 cgaaactaaa tcagttttta ttttttgctg cttaaaccag tcgagcattg ataccgtatt
     4501 accgttcact aatgtcagct gtaaatggta atttttgttc atcaggcgga aggttaattg
     4561 atggaagact tccagcacca acaaggtcat cactgaacca taaataccca gttcgtacat
     4621 accgctgcca ataaccatac ctatgcggcg gtcacccaga tatccgctgc cgtcgtcaga
     4681 cctacaatat tttggttacg aacgagaatg ttacctgcac cgataaaccc gacgcccgtc
     4741 accacctgag cagcaatacg gctggggtcg agtccgacgt gatctaaaga cagcacgtca
     4801 gcaaaaccat atttcgaaac aatcataaac agggcgcttc ccatgccaat taatacatgt
     4861 gtgcgtaac
//
LOCUS       DAEEDS010000187         4863 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEEDS010000187 DAEEDS010000000
VERSION     DAEEDS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.190"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..425
                     /locus_tag="KLH32_004052"
     CDS             <1..425
                     /locus_tag="KLH32_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0140666.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            759..3038
                     /gene="ydeP"
                     /locus_tag="KLH32_004053"
     CDS             759..3038
                     /gene="ydeP"
                     /locus_tag="KLH32_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBE0140667.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3286..3489
                     /gene="safA"
                     /locus_tag="KLH32_004054"
     CDS             3286..3489
                     /gene="safA"
                     /locus_tag="KLH32_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBE0140668.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3557..4318
                     /gene="ydeO"
                     /locus_tag="KLH32_004055"
     CDS             3557..4318
                     /gene="ydeO"
                     /locus_tag="KLH32_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBE0140669.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4720..>4863
                     /locus_tag="KLH32_004056"
     CDS             4720..>4863
                     /locus_tag="KLH32_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBE0140670.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1390 a         1038 c         1079 g         1356 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      541 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      601 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      661 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      721 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      781 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      841 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      901 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      961 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
     1021 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
     1081 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
     1141 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
     1201 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
     1261 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
     1321 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1381 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1441 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1501 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1561 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1621 aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct
     1681 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1741 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1801 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1861 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1921 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1981 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     2041 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     2101 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     2161 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     2221 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     2281 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     2341 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2401 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2461 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2521 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2581 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2641 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2701 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2761 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2821 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2881 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2941 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     3001 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     3061 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     3121 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     3181 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     3241 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     3301 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3361 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3421 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3481 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3541 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3601 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3661 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3721 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3781 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3841 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3901 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3961 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     4021 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     4081 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     4141 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     4201 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     4261 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     4321 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4381 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4441 tatttatgca cttacataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4501 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4561 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4621 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4681 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4741 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4801 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
     4861 acg
//
LOCUS       DAEEDS010000188         4658 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEEDS010000188 DAEEDS010000000
VERSION     DAEEDS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.192"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            69..437
                     /locus_tag="KLH32_004057"
                     /pseudo
     CDS             69..437
                     /locus_tag="KLH32_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            699..1061
                     /locus_tag="KLH32_004058"
     CDS             699..1061
                     /locus_tag="KLH32_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBE0140671.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1058..1978
                     /locus_tag="KLH32_004059"
     CDS             1058..1978
                     /locus_tag="KLH32_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE0140672.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1981..2932
                     /locus_tag="KLH32_004060"
                     /pseudo
     CDS             1981..2932
                     /locus_tag="KLH32_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2959..3199
                     /locus_tag="KLH32_004061"
                     /pseudo
     CDS             2959..3199
                     /locus_tag="KLH32_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3196..3501
                     /locus_tag="KLH32_004062"
     CDS             3196..3501
                     /locus_tag="KLH32_004062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140673.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3735..4175)
                     /locus_tag="KLH32_004063"
     CDS             complement(3735..4175)
                     /locus_tag="KLH32_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HBE0140674.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQ
                     DAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
     gene            complement(4147..>4658)
                     /locus_tag="KLH32_004064"
     CDS             complement(4147..>4658)
                     /locus_tag="KLH32_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140675.1"
                     /translation="CSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVK
                     TALENLGLGEAAKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPN
                     VSSTYGMRFPMTFSRVLAVTVTLNVTGASGQPTVSATNVQNTGFDITVSPGSGYGSSA
                     DAYYIAMGY"
BASE COUNT         1337 a          954 c          918 g         1449 t
ORIGIN      
        1 ccttcaggga aaaatatcat cagatggctg cttaaaaaaa gaacaaatgg tagtgtccgc
       61 tattgccagt acacctcagg gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa
      121 taaaaacagc agcggtaagg aagatgagaa ccgacagcaa caaggcatgg aagggagcgt
      181 taaagcgggc aggcattagc aacttccgct tccatgacct gaggcatacc tgggcaagct
      241 ggctggttca gtccggtgtc tctcttcttg cgcttaaaga gatgggagga tgggaaactc
      301 tcgaaatggt tcaaagatac gcccaccttt cagccgggca tctcaccgag cacgcgagca
      361 aaatcgatgc gattataagt cgcaatggca caaatacggc acaagaggag aacgtagttt
      421 acttaaatgc gaggtaactt attgatttaa atggtgccga taataggagt cgaacctacg
      481 accttcgcat tacgaattat aagaatccgc ttgtaattca aaggattatc ccatcaacac
      541 tgcgctcaca cgtcccacca catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct
      601 tatgtgtctc agttttgtct catcagacat agcaagtatc gatcaattga gacttggatg
      661 atagacttca tgcctttcag agctcattga ttaaataaat gttaaagtta tttgtaaagt
      721 acacatctat aggtgtgctg aacacactta tacactgggt ggtttttggt gtatgtatct
      781 ataccgcaca taccagtcag gctctggcaa acttcacagg tttcgtagtg gctgtgagct
      841 ttagcttctt cgcgaatgca agattcacat tcaaagcatc gactacagcg atgcgctaca
      901 tgttatacgt gggatttatg ggaatattga gtgtgattgt tggttgggca gctgataaat
      961 gctcacttcc tccaatagtc actcttatca ccttctccgc catcagtctg gtgtgcggtt
     1021 tcgtctattc aaagttcatt gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc
     1081 tgtcttcaat gaagaagaag cgatacctgt tttctataaa acggtacgtg aattccaaga
     1141 gttgaagcca tatgaagtag aaattgtatt cataaatgac ggaagtaaag atgccacaga
     1201 gtcaattatt aacgcgctgg ctgtttcaga cccgctagtt gttccgctgt catttacacg
     1261 caactttggt aaagaaccag ccttatttgc agggttagac catgcaagcg gcgatgctgt
     1321 aattcctatt gatgtcgacc tgcaagaccc aattgaggtt atccctcatc ttattgaaaa
     1381 gtggcaggca ggtgctgaga tggtgcttgc taaacgttca gaccgctcaa ctgatggacg
     1441 actgaaacgt aagacagctg agtggttcta taaattacac aacaaaataa gcaccccaaa
     1501 gatcgaggaa aatgtcggag attttcgact catgtctcgt gaggttgtgg agaacattaa
     1561 actgttgcct gagcgcaatc ttttcatgaa aggcatactg agctgggtgg gtggtcagac
     1621 ggatgtcgtt gaatatgtac gcgcagagcg tgttgctggc atctcaaaat ttaatggctg
     1681 gaaattatgg aatctggcac tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt
     1741 atggacttat ataggcttgt ttgttgcaag catttcattt ttatatggtg catggatgat
     1801 tatagacacc cttgtctttg gtaacccagt acgcgggtat ccctccctgc ttgtatcaat
     1861 acttttcttg ggtggagtgc aactgatcgg gattggtgtt ctcggtgaat atataggtag
     1921 aatttactta gaaacaaaat caagacctaa gtatattttg aagaaaggta gaaaataacc
     1981 atgagcatat gtattaaaca atcagcatta aaaatattat tggctttatc agcactccta
     2041 ataacttggt tgactaccag atactttcca gttgaaccgg atgttgctaa cagcccaatt
     2101 gtatggaggc atattttaga gaatggtata tcatcaatcc acgactggaa accaaccgtg
     2161 gataattggt atttcaccgt ttacccaatt aactttttat tttatatgct gttaggtgat
     2221 gatggcttag tagcattaag attatcaact gcagttttct ctattgctat agtaatagca
     2281 gcgatgttaa cgctacgtaa ggcattcggg tttacccctg ctttattttc tattatactt
     2341 ctatctctaa taccatattt ttcatacact tatggttttg tatcacatcc tttttctcat
     2401 aattcaacaa acgctttcgg ctttttatgc ttgcttatat ctgttttcaa catacagtat
     2461 aaaaatatat ttatcactct cctcttaagt ttgacagcat tattttccag cgtatccgat
     2521 ccgtggttta cagctgcatt ttttattcca ctattaatat cttacttttt attctctgta
     2581 tgggataaaa aattatttaa gcatactgca ttaatactat ttgcatgttt aatctctctt
     2641 tccaatgtat tgcaaaatct acttaacata cctccccacc aatttgaaat tgtatcactc
     2701 aatgatatga ttttaaatgc aaaatggtgc attctgttaa taggaaaaag tcttaattta
     2761 ttagttgtcg acaataacgc aacatcttac gcctcatttg tcatttggtt cattgccatt
     2821 attacatctg cgtggtttgt tttatcggac aataaaaaaa acacataccg tatttatatt
     2881 gtgttatttt cattattatc tatcgcaggt attgtctcat cattcatact gagctataaa
     2941 tcccctgatt atattagcat gcgtttcttt atgaatgtta cctgtttcgc actgatattg
     3001 tgctgtatcg gtacatcaac aaaagcaaag atattatttt atctaatcgc atttttattt
     3061 tcgatcagtt caattaaatc ttacacaaat aatgcttcac cattacacga tcaggagaag
     3121 attgttaaat catacattga ttttttaaaa aaaaataatc tccattatgg gtacggctct
     3181 ttttgggatt tgtcaatgac tgtaaattgg ttatctggtg gggacataca gataactcct
     3241 gttttcttta atgctgattc ggggaaaatt aactttactg gcgtgagaca acagacactg
     3301 gcttcttggc atagcaagga ggcgttcaat tctgctcctg agcgacagtt tatagcagtt
     3361 tccatagcta acgaacctga acgatgtaaa gaaatgacat cctgccttgc aggtattcaa
     3421 gaacaattag gtaagcctga cgaagttttg aactttgaag gaagagtaat attagtattt
     3481 aacaaaaaac ttaacctata gtaaattgcc ccctaccata taaaccacac taacgaatga
     3541 ataacccgtc tttttcgacg ggttatatcc ctatcaggaa taaaaatgga gtggttgccg
     3601 tatacaacaa accctcattt agagctatat tctagagtat atgagatact ataaagttga
     3661 aggagctatc attagaaaaa ccatatgctg ttataattag atgtctcgct tttcatccaa
     3721 taacgaattt tctcttatag attgctctct ggttttttcg gccactctgt attttttata
     3781 tcaacacgca tcaatagtgc gcgatatttt ttccatgcca gtaacagttt cgtttcatca
     3841 tctgttgcca tatctaaatc aaccgcatcc tgaagcggcg ctattacatt cgttgcctct
     3901 gctatcagtg cagatttctt ttcattattt attaaggcta attgttctgg tgttggagaa
     3961 ggacggtcaa caatgacagg gtgaccattc tcaccacagg taatcatttt tgttacactc
     4021 tgcgaattaa tcagtgtttt ccactctgat tccgagattt ccactgcatc atcaggaata
     4081 ttggtgccgt gaaattcagt cgaataaaat ccgtttgtcg atgcagaata aaaatatgac
     4141 atttcgttaa tatcccattg caatgtaata cgcatcagca gatgaaccat atcctgaacc
     4201 gggcgacact gtaatatcaa atccagtgtt ctggacattt gtcgccgata cagtcggctg
     4261 ccctgacgcg ccagtaacgt tcaatgtaac tgtgacagca aggactcttg agaatgtcat
     4321 cgggaacctc atcccgtatg ttgaagatac attaggggag ccattcaact gaccgcactg
     4381 taaaattaat ccagacggaa atttttgcgc agcagccgtt gcggtattac ttgacgcgaa
     4441 caggctcata tcaggtatct gattcgcccc tgtacctaca ttccttttag ctgcttctcc
     4501 caaaccaagg ttttcgagag ccgttttcac cgtgccatcc gatttgatat cgccaaacgg
     4561 attcttgcgg cttaacagca gcgcgcgaag tgcggtaagc aactgatcgt gccgcccctt
     4621 ctccaggctg gcaccggatg cctccaccac gctgcaaa
//
LOCUS       DAEEDS010000189         4641 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEEDS010000189 DAEEDS010000000
VERSION     DAEEDS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.193"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..311
                     /locus_tag="KLH32_004065"
     CDS             <1..311
                     /locus_tag="KLH32_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE0140676.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="KLH32_004066"
     CDS             468..1217
                     /locus_tag="KLH32_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBE0140677.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="KLH32_004067"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="KLH32_004067"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBE0140678.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KLH32_004068"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KLH32_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBE0140679.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="KLH32_004069"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="KLH32_004069"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBE0140680.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="KLH32_004070"
     CDS             4084..4557
                     /locus_tag="KLH32_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBE0140681.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1241 a         1163 c         1138 g         1099 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaattcaa a
//
LOCUS       DAEEDS010000190         4614 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEEDS010000190 DAEEDS010000000
VERSION     DAEEDS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.194"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            225..2648
                     /gene="pgaA"
                     /locus_tag="KLH32_004071"
                     /pseudo
     CDS             225..2648
                     /gene="pgaA"
                     /locus_tag="KLH32_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2657..4150
                     /gene="pgaB"
                     /locus_tag="KLH32_004072"
     CDS             2657..4150
                     /gene="pgaB"
                     /locus_tag="KLH32_004072"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBE0140682.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4154..4614
                     /gene="pgaB"
                     /locus_tag="KLH32_004073"
                     /pseudo
     CDS             4154..4614
                     /gene="pgaB"
                     /locus_tag="KLH32_004073"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1319 a         1035 c         1061 g         1199 t
ORIGIN      
        1 atttatgccg tattcctgaa gatcctcatc attggaatgg attttcgggc gagaaaagga
       61 ttttatatgg acactctgct catcatttct tcttctcatc atcaacaatt cacgtctctc
      121 ttccgcgttt aataacggat tatgaggtgc aaaaatatct ttcttttcag ttacctgtaa
      181 ttagatacag agagagattt tggcaataca tggagtaata caggatgtat tcaagtagca
      241 gaaaaaggtg cccgaaaacc aaatgggctt tgaaacttct tactgccgca tttttagcag
      301 cgagtcccgc ggcgaagagt gctgttaata acgcctatga tgcattgatt attgaagctc
      361 gcaagggtaa tactcagcca gctttgtcat ggtttgcact aaaatcagca ctcagcaata
      421 accaaattgc tgactggtta cagattgcct tatgggccgg gcaagataaa caggttatta
      481 ccgtttacaa ccgctaccgt catcagcaat taccagcgcg tggttatgca gctgtcgccg
      541 tcgcttatcg taacctgcaa caatggcaaa actcgcttac actgtggcaa aaggcgctct
      601 ctctggagcc gcaaaataag gattatcaac ggggacaaat tttaaccctg gcagatgcgg
      661 gtcactatga tactgcgctg gttaaactta agcagcttaa ctctggagca ccggacaaag
      721 ccaatttact cacagaagcc tatatctata aactggcggg gcgtcatcag gatgaattac
      781 gggcgatgac agagtcatta cctgaaaatg catctacgca acaatatccc acagaatacg
      841 tgcaggcatt acgtaataat caacttgctg ccgcgattga cgatgccaat ttaacgccag
      901 atattcgcgc tgatattcat gccgaactgg tcagactgtc gtttatgcct acgcgcagtg
      961 aaagtgaacg ttatgccatt gccaatcgcg ccctcgccca atacgctgca ttagaaattc
     1021 tgtggcacga taacccagac cgcactgccc agtaccagcg tattcaggtt gatcatcttg
     1081 gcgcgttatt aactcgcgat cgttataaag acgtcatttc tcactatcag cgattaaaaa
     1141 agacggggca aattattccg ccctaggggc aatattgggt tgcatcggct tatctcaaag
     1201 atcatcagcc gaaaaaagca cagtcaataa tgaccgagct cttttatcac aaggagacca
     1261 ttgccccgga tttatccgat gaagaacttg cggatctctt ttacagccac ctagagagtg
     1321 aaaattatcc gggcgcgcta actgtcaccc aacataccat taatacttcg ccgcctttcc
     1381 ttcggttaat gggcacgcct acgagcatcc cgaatgatac ctagttacag gggcattcgt
     1441 ttctctcaac cgtagcaaaa tatagtaatg atcttcctca ggctgaaatg acagccagag
     1501 agcttgctta taacgcacca ggaaatcagg gactgcgcat tgattacgcg agtgtgttac
     1561 aagcccgcgg ttggcctcgt gcagcagaaa atgaattaaa aaaagcagaa gtgatcgagc
     1621 cacgtaatat taatctggag gttgaacaag cctagacagc attaacgtta caagaatggc
     1681 agcaggcagc tgtcttaacg cacgatgttg tcgaacgtga accgcaagat cccggcgttg
     1741 tacgtttaaa acgtgcggtt gatgtacata atcttgcaga gcttcgtata gctggttcaa
     1801 caggaattga tgccgaaggc ccggatagtg gtaaacatga tgtcgactta accaccatcg
     1861 tttattcacc actgctgaag gataactggc gcggttttgc tggattcggt tatgccgatg
     1921 gacaatttag cgaaggaaaa gggattgttc gcgactggct ggcgggtgtt gagtggcggt
     1981 cacgtaatat ctggctcgag gcggagtacg ctgaacactt tttcaatcat gagcataaac
     2041 ccggcgcgcg cctgtctggc tggtatgatt ttaatgataa ctggcgtatt ggttcgcaac
     2101 tggaacgcct ctctcaccgc gttccattac gggcaatgaa aaatggtgtt acaggcaaca
     2161 gtgctcaggc ttatgttcgc tggtatcaaa atgagcggcg taagtacggt gtctcctggg
     2221 ctttcactga tttttccgac agtaaccagc gtcatgaagt ctcaattgag ggtcaggaac
     2281 gcatctggtc ttcaccatat ttgattgtcg atttcctacc cagtctgtat tacgaacaaa
     2341 atacagaaca cgatacccca tactacaacc ctataaaaac gttcgatatt gttccggcat
     2401 ttgaggcaag tcatttgtta tggcgaagct atgaaaatag ctgggagcaa atcttcagcg
     2461 caggtgttgg tgcctcctgg caaaaacatt atggcacgga tgtcgtcacc caactcggct
     2521 acgggcaacg cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa
     2581 aacgacctta tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca
     2641 gattttaagg ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat
     2701 aattatgctc accgcgtgca tcagccagtc aagaacatca tttataccgc cacaggatcg
     2761 cgaatcttta ctcgccgagc aaccgtggcc gcataatggt tttgtggcga tttcatggca
     2821 taacgttgaa gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg
     2881 tgaacaattt gcctggctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg
     2941 tgaagcacat cgaggaggaa aaccgctacc ggaaaaagca gtagtgctga cttttgatga
     3001 cggctaccag agtttttata cccgcgtctt cccaattctt caggccttcc agtggcctgc
     3061 tgtatgggcc cccgtcggca gttgggtcga tacgccagtg gatgaacaag taaaatttgg
     3121 cgatgagatg gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg
     3181 ttcccggctc gttgaggtcg cttctcatac atggaattct cactacggta ttcaggctaa
     3241 ttccaccggc agcttaatgc ctgtatatgt aaatcgtgca tattttactg accacgcacg
     3301 gtatgaaacc gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga
     3361 atacctgcgt acaaaggctg aggtaaatcc acacgttttt gtttggcctt atggcgaagc
     3421 gaatggcata gcgatagagg aattaaaaaa actcggttat gacatgtttt tcacccttga
     3481 atcaggtttg gcaaatgcgt cacaattgga ttccattccg cgagtattaa tcgccaataa
     3541 tccctcatta aaagagtttg cccagaaaat tattaccgta caggaaaaat caccacaacg
     3601 gataatgcat atcgatcttg attacgttta tgacgaaaac cgccagcaaa tggatcgcaa
     3661 tattgatgtg ctaattcagc gggtgaaaga tatgcaaata tcaaccgtgt atttgcaggc
     3721 atttgctgat cccgatggtg atgggctggt caaagaggtc tggtttccaa atcatttgct
     3781 accaatgaaa gcagatattt ttagtcgggt tgcctggcaa ttacgtaccc gctcaggtgt
     3841 aaacatctat gcgtggatgc cggtattaag ctgggattta gatcccacat taacgcgagt
     3901 aaaatactta ccaacagggg agaaaaaagc acaaattcat cctgaacaat atcaccgtct
     3961 ctctcctttc gatgacagag tcagagcaca agttggcatg ttatatgaag atcttgccgg
     4021 acatgctgct tttgatggca tattgttcca cgatgatgct ttgctttcag attatgaaga
     4081 tgccagtgca ccggctatca cggcttatca gcaagcaggc tttagcggga gtctgagcga
     4141 aattcgataa aacccggagc aatttaaaca gtgggcccgc tttaaaagtc gtgcgttaac
     4201 tgacttcact ttagaactta gtgcgcgcgt aaaagccatt cgcggtccac atattaaaac
     4261 tgcacgaaat atttttgcac ttccggtaat acaacctgaa agtgaagcct ggtttgcaca
     4321 gaattatgct gatttcctaa aaagctatga ctggaccgct attatggcta tgccttatat
     4381 ggaaggtgtc gcagaaaaat cggctgacca atggttaata caattgacca atcaaattaa
     4441 aaacatccct caggctaaag acaaatctat tttagaatta caggcacaaa actggcagaa
     4501 aaatggtcag catcaggcta ttccttcgca acaactcgct cactggatga gcctattaca
     4561 actgaatgga gtgaaaaact atggttatta tcccgacaat tttctgcata acca
//
LOCUS       DAEEDS010000191         4601 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEEDS010000191 DAEEDS010000000
VERSION     DAEEDS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.195"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..395
                     /locus_tag="KLH32_004074"
     CDS             <1..395
                     /locus_tag="KLH32_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140683.1"
                     /translation="LEYATTRKKSEVVYSGVSVTIPTAPTNLVSLLKTLTPSSGSLAP
                     FFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQLTFSGSVPDTLVSSRNAATT
                     TDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            395..850
                     /locus_tag="KLH32_004075"
     CDS             395..850
                     /locus_tag="KLH32_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBE0140684.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            853..1461
                     /locus_tag="KLH32_004076"
     CDS             853..1461
                     /locus_tag="KLH32_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBE0140685.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            1471..2802
                     /locus_tag="KLH32_004077"
     CDS             1471..2802
                     /locus_tag="KLH32_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBE0140686.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            2803..4533
                     /locus_tag="KLH32_004078"
     CDS             2803..4533
                     /locus_tag="KLH32_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBE0140687.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         1261 a         1135 c         1204 g         1001 t
ORIGIN      
        1 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
       61 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
      121 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
      181 aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc cagcggaacc gccaacaggt
      241 caatgcagct aaccttttcc ggctctgttc ctgacacact ggtcagcagt cgtaatgcgg
      301 cgacaacaac cgacaacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
      361 ttgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
      421 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
      481 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
      541 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
      601 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
      661 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
      721 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
      781 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
      841 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgtgcgg cgaggaacct
      901 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
      961 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     1021 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     1081 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     1141 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     1201 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     1261 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct gcaaggtcag
     1321 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     1381 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gtattggtgc tggtatcgga
     1441 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
     1501 ttgttggctg gtatcggcgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
     1561 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
     1621 ctgactgctt tgcaaggtct ttcaggtatt gcgggtgttt ttcagcagga aaagcaggct
     1681 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
     1741 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
     1801 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
     1861 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
     1921 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc aacagaaccc gcggcagttc
     1981 ggcgaatttg tcgatcacct ggggatgaac agtctcgggc ccgaaaaata ctttgaccta
     2041 caggataaaa tgcagggtcg ccaggttacc atgcgcggtc aggatctgga ttcgcaaacc
     2101 gccgctcgga atcaggcaat cacaatgcgc ggacaagata tccaggcgaa tttaggtcag
     2161 cagcgcatta atctggacgc agaaacaaac cgcattaaca acgaaaataa gcgccttgac
     2221 cggatgctat cagcagaaac taacgacctg aagcgccagg aaatacagag ccgcatagca
     2281 gccaacaacc agcagttgca gcagaagcag caagcgctaa atgatggcta caaagacggc
     2341 atcaacaccc tcacaaccag catgttcact ctgaacgata tcgttagttc tccttcactt
     2401 aagagcatta caggcttacg tggagtaatc cccaacgttc caggctcaca ggctgcagac
     2461 actcaggcac gacttgatac ctttaaatca caagcatacc tgacagcggt tcaggccatg
     2521 cgaggcatgg gcgcactttc tgatgccgag ggcaaaaagc tcgaccaggc tgttggttcg
     2581 ctgcagaact cgcagagcga ggagtccttt cgtcgcaacg ctggcgtcat cctgaacacg
     2641 ctcaaccaga agcgtaatga ggcggttggt aagtacgttc agcaaaacgg tatcaagcga
     2701 gtggaagcgc ctcaggcttc tatagattac ctgaagcagc accccgagct gtcaatcgac
     2761 ttcattaatc gctacggata tcttccatct ttggggcagt aaatggctaa ttaccgtgat
     2821 ttgttagagc aggctggcgc acgttacggt gtgccagaag ggttgatgac tgcactgggt
     2881 gccaaggagt cttcttacaa ccctgccgca gtaagctccg ccggggctgt aggattgact
     2941 caggtcatgc ctgggacatg gcgtgatatg ggttataccg atgagcaaat gcaaaacccc
     3001 gaatatcagg ctgacgctgg cgcgcgctat ctggcaaaga tgtaccagca gtttggtaac
     3061 tggcgtgacg ctcttcaggc ttatcacgac ggtcccggca acgttatgaa ggcaaagcgt
     3121 ggtgaatata cgccaggacc tgaaggccgc ggttacgttg atgatcgctt tgctcaatgg
     3181 gctggtgacc cggtgacaga ctcaacagtc gaacagcgcg ccacctctgc aaaggtacat
     3241 cctcagcaag accctaacaa cccgtttgca caactggaag tacagtcatc cgaacaagta
     3301 tcggcatcag gtgtgcagtc agacccaaat aatccatttg ctcagattga gcagcaggca
     3361 gccagtcagc agccacctca acccgtaagt tctgtcgcac cgaaacctgt tcagcaacaa
     3421 acagttaatc aggccaataa tgcaccaaca cgggaagaac catcattgat gcaacaagct
     3481 ggcgattggc tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt
     3541 ctggtaaaca taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt
     3601 caggggcttg gggggccaaa agtattggat gatgtttatc gtccagtaga cagaccgaca
     3661 gacccctacg cgcaagctgg agagtcaata ggcggttatc ttgttcctgg agcaggtgta
     3721 gctggaaaca tggtcattgg ttctctcgct gacgcggcga atcaacgggg tgattttgcc
     3781 gaaaatgccg ctattaatgc cggacttagc attgctacgc atggcctgat aaatggcgtt
     3841 acccgtggtg ttcgtggtgc atcaaatata attagtggca ataaaacatc tgcacagaga
     3901 gcgaccactg cgccaacaga aacatcacca ttctccggtg atgccgctgc agcaacaaat
     3961 cctgcggttc atgccgcaga ggcaagagta gcacaaggtg taccaatgac gcctgcgacg
     4021 aagaacccag aggaagtcgt tcgcacagta gcagcacaaa aaaggccaaa tctcgcttca
     4081 tcgcttgatg aactagatat caatcctcag gctgaagttc tggagtctgc tgaaaggctt
     4141 aatgttgatt cattactccc ttcacacttt tccgggaacg agcaatacaa ggcagttgag
     4201 caagcaatca agtcccgtgc gggttctgct ctacaggtgc aggaaaatga agcaatcagg
     4261 cagctagcac agggcgcggg tgagataatt gatcgcgttt ccggtgcaaa agatgctctt
     4321 ggtatgagcg acaagtttat tgatacggtc aatggaagaa tgtctgcgct gatgaaacga
     4381 agcgaccagc tttatcgcaa tgttgaaaag gcgatgcctg caggtgcaaa aattgatgcg
     4441 ccatcaacaa ggtcaatgct caaacaggtg gcagaagatc ttggcgggat gaaaaacctt
     4501 gaccctattg aaaagagagt tgaggtagcc tgagtttaac ggacactcct tcctgaaata
     4561 gaatggcatc agaaggagct aataatgagc agaaaaaccc a
//
LOCUS       DAEEDS010000192         4551 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEEDS010000192 DAEEDS010000000
VERSION     DAEEDS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.196"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1225
                     /locus_tag="KLH32_004079"
     CDS             <1..1225
                     /locus_tag="KLH32_004079"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBE0140688.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1225..1899
                     /locus_tag="KLH32_004080"
     CDS             1225..1899
                     /locus_tag="KLH32_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0140689.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2427..3188
                     /locus_tag="KLH32_004081"
     CDS             2427..3188
                     /locus_tag="KLH32_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0140690.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3232..4311
                     /locus_tag="KLH32_004082"
     CDS             3232..4311
                     /locus_tag="KLH32_004082"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0140691.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4308..>4551
                     /locus_tag="KLH32_004083"
     CDS             4308..>4551
                     /locus_tag="KLH32_004083"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0140692.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          865 c         1119 g         1318 t
ORIGIN      
        1 ctttccgtat ggcattttcc agcagcgaaa tgttcttgcg agctggctgg caacaggttg
       61 cggcgtagcg ttgtatctgg ctttaacggc gcgtactcgt tctctgacgt tgatatgttt
      121 gttgtcgtta taccctctgt ctacagcttt agtccttaca caaagccgtg tcggccttct
      181 tggtttattt tgcgtcatcg tgctggccgc tctcgcggac agaccccgct ggcgcggacg
      241 tccccttagc ggtctgttgc gcttgatctt cgtcgcctcc ttgctgtgct ggtgtgtcgg
      301 tatctcaaac tatgccatgc caggggagac aacggctgac tttacgcatt catcatcgaa
      361 tgaccagcgt atacgtattc tgaaaggtgc cataagtctt attcaacagc gccccttaac
      421 cggttggggg ttaggcagct ttgaagcgac cttcccgcaa gccttagagg atgtgggcgt
      481 gacaaacata gagagtgata cggctacgca tccccataac gaactgctgt tcgtaacggc
      541 tgaaggtggc atcgtcgcct tgtttggttt tttgctgcta tcaggcgttt ggctgtggcc
      601 gtgtatatgc cgcctgaaaa tgacaaacaa taacgcgcac catcaatgtg aaaagtggtt
      661 tattgcctta atggggttgc ctttagtcat tcatatgatg accgaatatc ctctctatac
      721 gtctgttccc catttgatgc tgttgctgct ccttttccgc atcggaatgc cggaaaacgt
      781 gatgaatcag gtacgtatac ctaaaacagt ccggttgggt attctgcctg tcattgtgct
      841 cattatgacc gccgcgctgg tcatcctggt agcgggattt gatgcacagc gggaactgac
      901 cagagctgaa gctgatatga acgaaggatt tatccctcgc cttccggaac atagctggaa
      961 atacctgacg caggcagaac gtcttgagcg cgatcagcat ctgcttattg cgaataaacc
     1021 ggggttcgtt cgcgatacgg aaagcatgtc tcttttcact gtgtggggaa ataaatggtt
     1081 ggcaatccac aatgatgcag acgtaagcgc tgcaatgata ttcattgcgc acaggcgcgg
     1141 tgattatctg acagctgaaa aaataagaaa acgcgcagct cgggtatttg taaatgacga
     1201 acgttttact cgcgggggaa actaatggtt aacgatctga cgtataagaa attagaaagc
     1261 acaaacatca actgtaatat tctctcgcca gaaaatacca catggacaaa tgtcacggtg
     1321 attggcctgg atctttttgc catttgcggc gtgtgcagtt tgctcaagcg tgttggggga
     1381 gtgaaacttc aggttttcac ttccttaccc actgatatga atgttctgac tgacagtagc
     1441 atcgtgatat gggtaaaaat gcgtcacgat ggtttgcctg aattagccgg acatattgtg
     1501 aaggtttgtc gtcgataccg tctgatgcgg caactcgtta ttagcgatgc tattcctgaa
     1561 ggaatgacga atgatatcgg gcctctttcc cgagtatggc ttactcatgg gagtgcaaaa
     1621 gtggatattt tattatcttc attacggaag gtgttaaatg ctcattgttt gcctggtcct
     1681 ttgtttataa aacgcctggg aagagtgcaa tggagggttc ttttactccg cgcgaagggg
     1741 attagcacac gtaaaattgc agaaatctgt ggtattggcc tcaagaccgt tagcgcccat
     1801 gagtccgcaa ttcgagaacg attaggcacc aggaataaaa gtgagtacgt ctggttagta
     1861 cgtaatgctc aacttataca acaggcgata cccgcgtagg gaagagatgc gtcaaggtat
     1921 gaaagatgta acaatatgcg aacaaagcaa aaagtataat tgatgcgtca tagataactc
     1981 tctttttcga tgacgaaatc gctgagcatg cgtggtgagg ttagtaatag atgggtgcta
     2041 tgttttgcca cgggtttgta gctgtgaaac ctggaaaaaa ataagaatta aaaatattta
     2101 aagagagtta ataaggttct ggttgtaatt gtcttggtca aaaaaaagat attgtcaatg
     2161 ttgtttttta aattttcagg ttttggttaa atatttgctt acaaatatag gtgatttttt
     2221 tattgtaggc ttttgaaggc tattataact gtgcgctaaa ttaatgcgtt gaataaaaga
     2281 aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat aatctaaaaa
     2341 agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac gcggatttat
     2401 cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc aaagtcgtat
     2461 tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat gtcaaagtga
     2521 ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc tatgaacaag
     2581 ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg cttgtgcaga
     2641 gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact ccgccactgt
     2701 tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg ccagctttac
     2761 aagacaggga aaaattacac tggctatgcg ccaccggcat tacgcctaaa aagagtgatg
     2821 tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac atccaggtta
     2881 acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc aagatggtta
     2941 atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg gtcaatcagt
     3001 cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt gagaaactag
     3061 attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc ggccaggata
     3121 ttaaatggtc tgtgattact gatgaaggtg gtgagagtcg tttgtttata tctcatttgc
     3181 aatcataatg tattacattt ttattgaata tttttatgcc atgcatattc catgttttat
     3241 cgactgtcat taattttgat aatggcctta cttgcgggtc agttaagtgc ccaggagtgg
     3301 tcatttgatt cttcacagtt ggaaggaaac gttagtgcgg acactgtggc aatgtttaat
     3361 cagggagagc aactccccgg aaattatcgt gtggaaattt atcttaacgg tgaaaaagtt
     3421 gatgttgggg agtttccctt tcatcgtccg gaatcccctg aagaaaaaga gcttgttcca
     3481 tgtttaaccg ttgatgacct tatccactat ggcattaaaa ttgataaaag cagcagtgat
     3541 actgacaata aaaagaatca gtgttttaaa tggaactcta ttgaaggttt gaaagtaaat
     3601 tatgattttg atagccagcg cgtacagata accgttccac aactgtatct gcaagataaa
     3661 aaaagtagtt tagcaccagt gtcgttatgg aatgagggtg tagctgcatt ccgcatggtg
     3721 tatcagacaa atattgatat ttccaaacaa aatgataatc aatctaccac gcgtaattct
     3781 cgttatggca ggttcactcc agggtttaat cttggtgcct ggaggtttcg tagttcagtg
     3841 acatggagca aggaacttgg ccagtcagaa agatggcaaa ggggttacat gtggtttgag
     3901 cgaggcataa atgcgattaa aagccgatta acgcttggag aatcttatac gtcgtctgag
     3961 gtgtttgaca gcataccttt tcgcggcggg atgttagcga ccgacgatgc gatgactccg
     4021 ccggaggaca gctactacac acctgtggtg catggaatag cacaaagtga ggcgcaggtt
     4081 attattaaac aaaatgggca aattattttt acccgttctg tgccgccagg gccttttgcg
     4141 ctggataatt taccaacgct ggccgttggc ggggagcttg atgttacagt gagagagagt
     4201 aatggcgagg agcagtattt ttccgtgcca tttcaaacgc cggccatcgc tctgcatgaa
     4261 ggatatttca aatatagtgt gatggggggg aatataaaga agaaggtata aaaagctccc
     4321 ctgtctccgt gggcgaaatc acggcgatgt acgggcttcc ttatggcgtc acggtgtatg
     4381 gagggattca aaacgccact cattttaatg caatatctac gggtattggc atttctctgg
     4441 ggctgttagg gagtttatcg acagatatta cgcgttcgat tgcgaattta tattatggga
     4501 ataaatatcg tattcgttat agtaaatcca ttagtgattt tggtactcaa t
//
LOCUS       DAEEDS010000193         4514 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEEDS010000193 DAEEDS010000000
VERSION     DAEEDS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.197"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..151)
                     /locus_tag="KLH32_004084"
     CDS             complement(<1..151)
                     /locus_tag="KLH32_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140693.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVW"
     gene            complement(203..541)
                     /locus_tag="KLH32_004085"
     CDS             complement(203..541)
                     /locus_tag="KLH32_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBE0140694.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGG"
     gene            complement(541..2618)
                     /gene="flhA"
                     /locus_tag="KLH32_004086"
                     /pseudo
     CDS             complement(541..2618)
                     /gene="flhA"
                     /locus_tag="KLH32_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2611..3758)
                     /gene="flhB"
                     /locus_tag="KLH32_004087"
                     /pseudo
     CDS             complement(2611..3758)
                     /gene="flhB"
                     /locus_tag="KLH32_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3994..4377
                     /locus_tag="KLH32_004088"
                     /pseudo
     CDS             3994..4377
                     /locus_tag="KLH32_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(4387..>4514)
                     /locus_tag="KLH32_004089"
     CDS             complement(4387..>4514)
                     /locus_tag="KLH32_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140695.1"
                     /translation="ITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
BASE COUNT         1118 a         1294 c         1104 g          998 t
ORIGIN      
        1 cccagacacg cagagtctcc ggcgtacagc caatctttgg agcaatggaa caaattgtcg
       61 cccattgtga gtcatattcg ccctgacttt ccagaaccat acggactgcc cgttgacgga
      121 cttcggggga aaaacgagta tttttagtca tcctgtttac ctctttctca ggaagtttag
      181 tctccaggat tcccggggcg gttcacccac cgcctggcac ttcccagata aatcgcaacg
      241 gttctgctgc cgctataccg gaaaaggcca cggtggtacc gctctgcccc tctaattcga
      301 cacaacgaga ttgcgaacac aagcgaaccc gcagtcctgc aggtgtcggg ccgataagct
      361 gataacgcca cgccaccagc gtcatcaatc ctgaagcagg ttgtcgcgca gaaagaggtg
      421 cagacgacat cgactcaccg cgatgattta gcgtaattcc gacactactt gcctgccaca
      481 tcccctcccc ggcggcttgc accagcagcg gaaacaataa tatcgctaat aaggttctca
      541 tcatttaccg ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt
      601 cgacaggacc actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcag
      661 cgcgtggttc accaacaata ctggcggcgc acccagcatc tcctgacggg atagagcttc
      721 ctgagtttgc gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag
      781 cgcctgtagt aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt
      841 gccaggaaac cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa
      901 ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat
      961 atcgcgaatc ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag
     1021 cgtgacgacg ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc
     1081 caacagctgt tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg
     1141 gttaagatgc gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc
     1201 ctgttctttt agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt
     1261 cgcctcacca ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg
     1321 ataagcatca ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg
     1381 cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa
     1441 tttcttgcgg atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat
     1501 cgggatcagt cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca
     1561 cgtcgcttcg acaacggtat tattctctgc catttttacc ggtttgggtt cggcaggcgc
     1621 tttttgttcg cgtccgcgta tccaccaggc cagccccagc aatccggcag tgaacagcaa
     1681 aaataccagg ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa
     1741 caacataacg cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc
     1801 ggtgctgaca cgcgtaacga tgccccccgc ggcggtagaa atcaccagcg ccggaatttg
     1861 tgccaccaga ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct
     1921 catgccagtt gcagcacgcc gaccagcaac ccgccgacaa cgttaatgac catgatgagg
     1981 atcccggcaa tggcatcgcc gcgaacaaac ttacttgccc cgtccattga gccgtaaaaa
     2041 tcggcttcct gagtcacttc ggagcggcgt tttttcgcct catcttcacc aatcaatcca
     2101 gcgttaaggt cggcgtcaat cgccatctgc ttacccggca taccatcgag aacaaagcgc
     2161 gcacccactt ctgctatacg cccggcacct ttggtaatga ccataaagtt gatgatcacg
     2221 agaatgacaa acaccacgat accgatagcg aaattgccac caacgaggaa gtgaccgaac
     2281 gcttcgacca ccttcccagc cgccgccgcg ccggtatgcc cttccatcaa aatgatacgg
     2341 gttgaagcca cgttaagtgc cagacgcaac aacgtagtaa acaacagaat ggtcggaaac
     2401 gcagcaaact caagcgtgcg ctgggtaaac atcgccacca gcaacaccat gatcgacaag
     2461 gcaatattga aggtaaacaa caggtcgagt atgaatgcgg gcagtggcag caccatcatc
     2521 gacaagatca acaggatcaa aatcggtccg gcaaggatct gccattatgt cgatttcagg
     2581 tttgcgggca ggcgcagcat cgcggccaga ttactcatgg gtcggtttct cgttaataaa
     2641 atccagggct gccggcaccg gaagatgagt aggtgtacag ggcgctgtcc accagccaga
     2701 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
     2761 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
     2821 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
     2881 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
     2941 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
     3001 cgccgtgcgg cagctcgctg catctgacag atccgtcctt taacatgggg gtcgccttcg
     3061 ccttgtttga actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg
     3121 aagatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
     3181 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggtga ctcggccatt
     3241 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg
     3301 atggttttca aaattgcctt aagcaactcc gcgctagtct gtgccgagaa tatccgttta
     3361 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
     3421 agccccccca gcataaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggt
     3481 agcagcgcca gcatggcttc tctgatcagc agaataattt gcccgaggat cagattcggg
     3541 tctttgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
     3601 gccagcgaca caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag
     3661 gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt
     3721 gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta
     3781 ttaagcgtaa atgatgccag agcgcaaagc gttcaatggt ttaagtaagg ggcaaaacag
     3841 gcgggattta gggcgtttat gcaaaaatag gttcagttgc agaacgtttt gcgaaccatt
     3901 ttccagttct gatatttagc catttagcct atagaatcgc actccgcctc tctgttagtt
     3961 tgctaataat taaattatta caaaggtgat caaatggaat ggcaacaatt gcttaactgc
     4021 aaccgtcgga aagataatct aaaaaaaacc aacatagcat ttttacatcc ccttgctagc
     4081 ggaagacaag agatcgagcg ggattacgac cgattacttt ttgctgcacc aactcgtcgg
     4141 cttgcggata aaacacaagt tttcccgtta gatcaaaatg acagcatacg gacgaggctg
     4201 actcattctc acgaagtagc aaactttgct cgcgggatag gaatgaggct cgctttcgag
     4261 atgaaggagc acatctttgg cgaaataccc acggggattg tcgttgaacg cgacgttcct
     4321 gctctgttag cagcgatcgg actggcacac gatctcggta atccaccatt tgggcatctg
     4381 aagtggtcaa caaaaactgg ccaccgagtt agagtttttc cagtatcgat tttccgattc
     4441 gtttgggggt aacccaccgt tatattcgtg cggtcttagt gcactgtaat atccaacgat
     4501 atagtccgtt atgg
//
LOCUS       DAEEDS010000194         4464 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEEDS010000194 DAEEDS010000000
VERSION     DAEEDS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.198"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(139..1041)
                     /locus_tag="KLH32_004090"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="KLH32_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="KLH32_004091"
     CDS             1101..1379
                     /locus_tag="KLH32_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140696.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KLH32_004092"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KLH32_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBE0140697.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KLH32_004093"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KLH32_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBE0140698.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="KLH32_004094"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="KLH32_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBE0140699.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="KLH32_004095"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="KLH32_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBE0140700.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..>4464
                     /locus_tag="KLH32_004096"
     CDS             4371..>4464
                     /locus_tag="KLH32_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBE0140701.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1312 a          954 c          943 g         1255 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggt
//
LOCUS       DAEEDS010000195         4341 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEEDS010000195 DAEEDS010000000
VERSION     DAEEDS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.199"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..782
                     /gene="lsrF"
                     /locus_tag="KLH32_004097"
     CDS             <1..782
                     /gene="lsrF"
                     /locus_tag="KLH32_004097"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBE0140702.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            806..1096
                     /gene="lsrG"
                     /locus_tag="KLH32_004098"
     CDS             806..1096
                     /gene="lsrG"
                     /locus_tag="KLH32_004098"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBE0140703.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="KLH32_004099"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="KLH32_004099"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBE0140704.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="KLH32_004100"
     CDS             complement(1897..2811)
                     /locus_tag="KLH32_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBE0140705.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            3012..3200
                     /locus_tag="KLH32_004101"
     CDS             3012..3200
                     /locus_tag="KLH32_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140706.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            3210..>4341
                     /locus_tag="KLH32_004102"
     CDS             join(3210..4026,4026..>4341)
                     /locus_tag="KLH32_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE0140707.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAG"
BASE COUNT         1081 a         1053 c         1216 g          991 t
ORIGIN      
        1 tggattgggg aatgcagtca cgcttatcgt ggatatttaa tccgaaaacg ggtaaaactg
       61 tgatgctggc ctttgaccat ggttattttc aggggccgac taccggactt gaacgcattg
      121 atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg cgcggcattt
      181 tgcgcagcgt agttcccccg gcgaccaata agccggtggt actgcgggcg tcaggtgcga
      241 actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat gatgccgtgc
      301 gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagcgaatat gaacatcagt
      361 cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa agtgggaatg ccgaccatgg
      421 ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg ttatttctcg ctcgcgactc
      481 gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta ttatgtcgaa aaaggttttg
      541 aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa aaattaccgg
      601 agcgcgagac gctggaaatg tgctggcagg ctatcgatca gggcgcttct ggtgtggata
      661 tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc gtacaggcgg
      721 tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt gaaaaacagt
      781 aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta acgttcatga
      841 agacaaggtt gacgagttta tcgaagtttt tcgccagaac cacctgggct ctgtacagga
      901 agaaggtaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc gcttttatat
      961 ttacgaagcc tataaagatg aagacacagt ggcattccat aaaaccacgc ctcactacaa
     1021 aacctgtgtc gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac gtctgttcaa
     1081 tgggttgatg ccgtgaggct acaattgggg taacgcgctg acgggagtaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagctcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gttcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc tagaaccacg gcagcctgac ggctgcaaat tgaaaactgc ccctgttcgt
     2941 cctgttgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt ttattaagga
     3001 ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct gttggtgctg
     3061 agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg gacccaccac
     3121 ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc cttacccggc
     3181 tacaggatac cgggggttaa tccgtcctca tgttgtccgc ttttactccc cgcccgctga
     3241 aacgtctgtt cacggccaac cagtgctgga catccttcct ggatgcgggc ggtctgcgcg
     3301 atatcgaggt tgaggctgtc acaaaaatgc tggcctgcgg cacgcggata ctgggcgtga
     3361 aggaatacat ctgcgataaa cctgagtgcc cccacgtcag atacgtcact aactcatgcg
     3421 gcagccgtgc ctgcccgtcc tgcggaaaaa aggccacaga cctgtggata gcgacacagc
     3481 tgaatcgtct tcctgactgc gactgggtac acctggtctt caccctgccg gacacgcagt
     3541 ggccggtgtt cgaaagcaac cggtggctgc tgaatgacgt gtgccgtctg gcggtggaga
     3601 atctgctgta tgccgcccgg aaacgggggc aggaacccgg tatcttctgc gccatccaca
     3661 cgtatggccg tcgtctcaac tggcatccgc atgtacatgt gtctgtaacc tgtggaggtc
     3721 tgaataagca tggtcagtgg aaaaagctga gcttcctgaa agacgcgatg cgttcgcggt
     3781 ggatgtggaa tatgcggcaa cggcttctga aagcgtggtc agaggggctg gcgatgccgg
     3841 agtcgttgtc acatatcacg acggaatcac agaggagaag cctggtgctg aaagccggag
     3901 gaaaatactg gcatgtgtac atgtcgaaaa aaacggccgg cgggaggaat acggcgcgct
     3961 acctgggtcg ttatctgaag aagccgccga tagcggcctc ccggctggca cattacaacg
     4021 gagggggagc ctgagcttcc gttacctgga ccacaaaacg ggagaaacgg cgacggaaac
     4081 gctgacacag cgtgagctgg tcgcgaggct gaaacagcac atcccggaga agttttttaa
     4141 gatggtgagg tacttcgggt tccttgccaa ccgggtgtgt ggagagaagc tgccgcaggt
     4201 gtaccgtgct ctggggatgg ataaaccgga accagtggcg aaagtgtgct atgcacaaat
     4261 ggtgaaacag ttcctgagtc gtgacccgtt cgaatgcgtg ctgtgtggcg gccggatggt
     4321 ataccgccgg gccatcgcgg g
//
LOCUS       DAEEDS010000196         4325 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEEDS010000196 DAEEDS010000000
VERSION     DAEEDS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.200"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..159)
                     /locus_tag="KLH32_004103"
     CDS             complement(<1..159)
                     /locus_tag="KLH32_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0140708.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(309..470)
                     /locus_tag="KLH32_004104"
     CDS             complement(309..470)
                     /locus_tag="KLH32_004104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140709.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(467..655)
                     /locus_tag="KLH32_004105"
     CDS             complement(467..655)
                     /locus_tag="KLH32_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBE0140710.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            915..1250
                     /gene="iraM"
                     /locus_tag="KLH32_004106"
     CDS             915..1250
                     /gene="iraM"
                     /locus_tag="KLH32_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBE0140711.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1531..1639)
                     /locus_tag="KLH32_004107"
                     /pseudo
     CDS             complement(1531..1639)
                     /locus_tag="KLH32_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2162..2238)
                     /locus_tag="KLH32_004108"
     tRNA            complement(2162..2238)
                     /locus_tag="KLH32_004108"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2202..2204),aa:Arg,seq:tct)
     gene            complement(2340..2415)
                     /locus_tag="KLH32_004109"
     tRNA            complement(2340..2415)
                     /locus_tag="KLH32_004109"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2380..2382),aa:Ile,seq:cat)
     gene            complement(2560..3381)
                     /locus_tag="KLH32_004110"
     CDS             complement(2560..3381)
                     /locus_tag="KLH32_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0140712.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3396..3752)
                     /locus_tag="KLH32_004111"
     CDS             complement(3396..3752)
                     /locus_tag="KLH32_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0140713.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3765..>4325)
                     /locus_tag="KLH32_004112"
     CDS             complement(3765..>4325)
                     /locus_tag="KLH32_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE0140714.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1188 a         1179 c          856 g         1102 t
ORIGIN      
        1 cagatatgtc agcagcccaa acagcagact ccccagcacg cctattgccg cccactgaga
       61 cggggaaacc ctgtccagca actgcaggaa ccagtagccc gttcccaccg ctgacgtggt
      121 gtatgacaca cctgttgtga ttttttccat ctggtccata ccccgtctcc cgttatccgg
      181 aagctgacaa caataaaaaa gccaccagtt aactactgat ggctctgata actcatgcaa
      241 gcgtctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttgactgg
      301 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      361 ccatacgttg catttttggt aatatccgtc agcgtcagtg catttagtcc cactgccaga
      421 ctgtctttta tggcctggaa tgccgggcca gtacgatgac gtagtatcac tccggctcag
      481 ttgcaccact gaccaccaca tcaccttctg ctgcaatcgc ctgcatcagg gtataagggg
      541 ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt gtcagctttt
      601 ccgcaagatc tgacggcggc gccgccctga caacatcata gtgtttaaat gccatggttc
      661 tttccaacat ctgaaaaata attctttaaa atacctgaca tgtaatacag aaaaaacaca
      721 aaaccatacc ttaaataaaa acctgattat caagcagata tgcatggata aactacaaga
      781 cgagatataa accaccctgc atttaaataa acaataaaca acatcagaaa aataattctg
      841 ctctatggtt taattcaaaa atatcattta tacttttcag aacatcacca gcaaggcata
      901 aacaaggaaa ctaaatgaag tggattgtga ttgatacagt tatccagcca tcatgcggaa
      961 tatctttttc agtcatatgg agtaaaataa aattaataat ctggtatcaa tcggatgctt
     1021 tcttacctcc tgaaagtata tttacactga ctcacacagg catcatgctc aataacaaag
     1081 tgctacctgt aaccatttac aacgtagtac cattcaataa aacattctgg aatttaatca
     1141 aaaacagcca ggaatgccct acaaatacag ataacgtatt gaatgaatgc tttaataacc
     1201 gttgcactct gcaaatatgt ccttatggac taaaacaaca aagtccataa ggagtttact
     1261 cacatctgac aaaatcaata taaacagccc ctccggagag gggctggaga gtggcgctat
     1321 gtgccattcc atggtgccgg gtgcctcccg gtgaattcag taccagcacc tgaatccgcg
     1381 attatcccat atacctactc gctgattgcc cctccgcaca gggggattca ccatgccagt
     1441 ttcttttaac aaactccccg caaaccagac aacagtcaac cgcctgaatt gtgaagtatt
     1501 taaaaatttc tcccgctaac tgatacccgg ctaacagtct ggcgttttct ttttcagcaa
     1561 cgggaaagca acaaccacca cacccgccac cagcacaccg tcagccggca ctgacattat
     1621 ccggctgctg caatgccatt aacaaaaaca gtaagcaatc actttttacc gtaacaggtg
     1681 ataatccaga tatgtatcta ccccagatga gtaatccgaa gttcatccat accacaggtc
     1741 ctggctattc tgttgtactc ctgaacaaga gcaaataatt ctgaattagc aaccatgaac
     1801 tcatcgcaaa ccctctgtat agcatcacta ttcagaataa taacgtctct tcccgaaaga
     1861 cgatcaggag tacagaacaa aactgtcaaa cggctgaagg cctttgctcg tgctgcattg
     1921 actatatcaa tacgctgcct aaggatgaaa caccccgacg cctcatcaat attcactcta
     1981 cccacaccat atgaatgata aatacttaat gctgaaaaaa ccattagacc gtataacaaa
     2041 tactcaatca atacttaaca gaacttttat ttttgacaaa tatataatat tttcaacaat
     2101 atcctgagcc aggtatattt cagtataagg ctctgccgga aggaatctgg aagaatgaat
     2161 atggcgcgct gtactggatt cgaaccagtg accgattgct tagaaggcaa ttgctctgtc
     2221 cggctgagct aacaacgctg aataccgata atggaccgcc atcggggacc cgccccccgc
     2281 accaacaacc ctgttatcgt gtcgtctgct cttcctgata agctaatggc ggtttgtgat
     2341 ggtggccctt gctggatttg aaccagcgac ctggcgatta tgagtcgctc gctctcacca
     2401 ctgagctaaa gggccgggag cagaataata acggtccgta attaatttcg caataaaaaa
     2461 acccgctcag cggcgggttg tagaaactct tctaacgcca ggcataaaac gcccatcatt
     2521 atgacgaatt taccacagat tccggaaaaa tcaaccttgt tacctagtta ccttttttaa
     2581 ctgccgctca gcccatgctt cttcaatatc aaaccgggtc accagcgcat cataaaattt
     2641 cttaactgtt ttttcccatg acgcgcgtgt tatctggttt gtcacctcgc atatagcatt
     2701 aaatgcctcc gttgatggta gtctttcata gccacgacca ccacaacgct ggcagtctct
     2761 gataacaggc ataccacgtt ttaccgactc ttcacgatga atggcgacac cacgcccacg
     2821 gcaatcctta caggcggtgg aaacctcacc ctttccgcca cactccggac aggcaacttt
     2881 taccacctcc ctgacttttt tccattcttc ccagtaagac ggatacacac ctttcgtaca
     2941 ctttgcccat accggcggct taccatccgg atactggatc ttgtttgtaa aaactatgct
     3001 ttcaataaat ttttccccat agcaacaagg gcactgcttt ttactcgctg cgctgcgggc
     3061 ataatcctca aaagcgtacg aagccataat gcgcatcact accggtttta tttctgccgg
     3121 aagttttctc aacgccgcca cacgatcgca ccgactgagt gcataatctg ccagtaattc
     3181 tgttgcccgc gccctgtcat tcatactgat gcccattttc cccaggaacg cagaaaaacc
     3241 catctcagcc cgattctgtg tcatgccctg cgcggccatc acatcagtga tactcagcgc
     3301 atcttttgac gttgaggccg atgcatcggt caggccaggg gattttgggg agtagtattt
     3361 cggtaaatct tccagtttca ttttttgacc tgctcttaat gcattatggg gtaaatcttc
     3421 acccccagac gtccaccaga tactggctga ccacgaacga tattgatttc atcaaactgc
     3481 tcatcgtcca ttaacactcc cgcatgcgtc agcgcatcca gcggtgcttt caggatattg
     3541 tccaggtcgc gacgacgctt atccggtggc tctgcaatca cctttatcgc cagccttccg
     3601 gacaggctta atttcagccg ctgctggcga acaataagcg ccacagcccg gcgataacgc
     3661 tttccctcct ccgagataaa atatgtgctg ccacggcgtc gccagtaagt gttcaccgtc
     3721 ggcgggtaag gtaaaaccaa atctatgagc atcagtcacc tcttttaccc aagcacgcca
     3781 gttgcaaagg cgtgatcaag aaaacgaaaa attaaatcaa cctgagaacc atgcttttct
     3841 tcgaacgcca gaggatccgc atgaagctcg ttgtgatgct cccgacacag cggtagcgtg
     3901 aaaatatcgt gagatttggt ccccattccg ccctgaccat gaccaatcag gtgatgggga
     3961 tcgtcggctg gcttaccaca acacgcacac ggctgtgtct tcacccagcg tttgtatttc
     4021 tcgttaaccc agcggcgacg tttaggtcgt ttcatgaaag attccggaga ctcaggatca
     4081 acggcaatgc tgaccaccgt cttttcctgt tgtgggttct gttgctggtg ggcgtgaggc
     4141 agcggcgcaa gattttttgt gcgctgtttc agtatgctgg tggcggtctg ctctcccggt
     4201 acgatgtcgc tttcacggta cattgagcgg attttttccg cacgcaaccc cagcgaacga
     4261 cgtaataccg cttccggtag cgcgtccgcc acctgattgc ggaccgccca ccaggataat
     4321 tcagc
//
LOCUS       DAEEDS010000197         4248 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEEDS010000197 DAEEDS010000000
VERSION     DAEEDS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.201"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            35..916
                     /gene="yddG"
                     /locus_tag="KLH32_004113"
     CDS             35..916
                     /gene="yddG"
                     /locus_tag="KLH32_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBE0140715.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1171..2271
                     /gene="ompC"
                     /locus_tag="KLH32_004114"
     CDS             1171..2271
                     /gene="ompC"
                     /locus_tag="KLH32_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE0140716.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2435..2920
                     /locus_tag="KLH32_004115"
     CDS             2435..2920
                     /locus_tag="KLH32_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBE0140717.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3030..4019
                     /locus_tag="KLH32_004116"
     CDS             3030..4019
                     /locus_tag="KLH32_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBE0140718.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1319 a          875 c          804 g         1250 t
ORIGIN      
        1 aatttttaac ggcaagagag acaaaacagc gagcatgaca cgacaaaaag caacgctcat
       61 agggctaata gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg gtgtcagcga
      121 ggggctcggc ccggtcggcg gcgcagctgc tatctattca ttaagcgggc tgctgttaat
      181 cttcacggtt ggatttccgc gtattcggca gattccgaaa ggctatttac tcgccgggag
      241 tctgttattc gtcagctatg aaatctgtct ggcgctttcc ttagggtatg cggcgacccg
      301 tcatcaggcg attgaagtgg gtatggtgaa ctatctgtgg cccagcctga caattctctt
      361 tgccattctg tttaatggtc agaaaaccaa ctggctgatt gtaccaggat tattattagc
      421 cctggtcggc gtctgctggg tgttaggcgg tgagaatggg ttacattatg atgaaatcat
      481 caataatatc accaccagcc cattgagtta tttcctggcg tttattggtg cgtttatctg
      541 ggcagcctat tgcacagtaa cgaataaata cgcacgcgga tttaacggaa ttaccgtttt
      601 tgtcctgcta acgggagcat gtctgtggat ttactatttt ctcacgccac aaccagaaat
      661 ggtatttagc acgcccgtca tgattaaact catctctgcg gcatttacct taggatttgc
      721 ttatactgca tggaatgtcg gtatattgca tggcaatgtc accataatgg cggtaggctc
      781 gtattttacg cctgtacttt cctcagcgct tgcagccgtg ctgctcagcg ccccgctgtc
      841 gttctcgttc tggcaaggcg cactaatggt ctgcggcggt tccctgctct gctggctggc
      901 gacacgtcgt ggttaaaata attgccgggt ctacggcccg gcagttaatc agataaattc
      961 cgacaatatt aaaaaataaa tttcaaatac cagtaattca ccttatatct catttcgact
     1021 tcgatcacat atcgataaat aatattattc tatttttctc attgttttta atcaccattc
     1081 tattttaaaa acagtatgtc atttataaat gacgccgttt aaaattcgtt tagaaaatac
     1141 gtttaacgat aattaaaagg attctgttaa atgaaattaa aaatagttgc ggtggttgta
     1201 actggtttgt tagctgcgaa cgtagcacac gctgccgaag tctataacaa ggatggtaat
     1261 aaactcgacc tttatggcaa ggttaccgct ctacgttatt ttactgatga taagcgtgac
     1321 gatggtgata aaacttatgc ccgtctcggc tttaaaggag aaacgcaaat caatgatcaa
     1381 atgattggtt ttggtcactg ggaatatgat tttaaaggct ataacgatga agccaacggc
     1441 tcgcgcggca acaagacccg tctggcctat gctggtttaa aaattagtga atttggctct
     1501 ctggactatg gtcgtaacta cggtgtcggc tacgacattg gttcatggac cgatatgttg
     1561 ccagaatttg gtggcgatac ctggagtcag aaagatgtct tcatgacata ccgtaccacc
     1621 ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa ttgaagggct gaactttgcc
     1681 gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc atctttatgg tgctgactac
     1741 acgcgtgcca acggtgacgg tttcggtatc tcctcaactt atgtttatga tggctttggt
     1801 atcggtgcgg tatataccaa atccgatcgg acaaatgcgc aggaaagagc cgcagctaat
     1861 cctctcaatg cctccggtaa gaatgcagaa ctatgggcca caggtataaa gtatgatgcc
     1921 aacaacatct actttgcagc taattacgat gaaacattaa acatgaccac ctatggggat
     1981 ggttatatct ctaacaaagc acaaagtttt gaagtggtgg cgcaatatca attcgacttc
     2041 ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca gagatctggg ccgctacgcc
     2101 gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt atttcttcaa caaaaatatg
     2161 tcgacctatg ttgattataa aatcaacctg attgatgaaa gcgactttac ccgtgccgta
     2221 gatattcgca ccgataacat cgtcgctacg ggcattacct atcagttcta acgtcctctc
     2281 ctgaccagcc cttctctggg ctggtttttg atttaagtgc tgctgtatga ctccttaagt
     2341 aaaaatgagc aattcttgat atttctggtc attcacctca attaatatta ttccatatcc
     2401 tccggggaca tcttactata atatttattg atgagtgatt atgaccaata ttaatacagc
     2461 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
     2521 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
     2581 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
     2641 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
     2701 aaatctggaa aacaattgtc taacttgtct tgacttagct gacaatgcca gtctcgttaa
     2761 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
     2821 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
     2881 ggttaatctg gaagcgcaaa ataatagcct aaaaaaatga tatttctgat agttataaac
     2941 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
     3001 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
     3061 ccaggatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
     3121 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
     3181 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
     3241 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
     3301 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
     3361 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
     3421 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     3481 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     3541 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     3601 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     3661 caggtaataa aaatgaactc ttactgctag atctatccca taataaacta acaagtttac
     3721 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     3781 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     3841 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     3901 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     3961 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgac
     4021 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     4081 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gcggataacg
     4141 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     4201 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacct
//
LOCUS       DAEEDS010000198         4099 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEEDS010000198 DAEEDS010000000
VERSION     DAEEDS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.202"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(954..1145)
                     /locus_tag="KLH32_004117"
     CDS             complement(954..1145)
                     /locus_tag="KLH32_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE0140719.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="KLH32_004118"
     CDS             1209..1532
                     /locus_tag="KLH32_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBE0140720.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="KLH32_004119"
     CDS             1522..1833
                     /locus_tag="KLH32_004119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140721.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2119
                     /locus_tag="KLH32_004120"
     CDS             1808..2119
                     /locus_tag="KLH32_004120"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBE0140722.1"
                     /translation="MHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLV
                     NHYYGLHDPNAPENRRTLTLPDNLPETKQAIAEGVTRGLPCRRDKRPSGCDSGSHRGR
                     D"
     gene            2200..2730
                     /locus_tag="KLH32_004121"
                     /pseudo
     CDS             2200..2730
                     /locus_tag="KLH32_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2737..2970
                     /locus_tag="KLH32_004122"
     CDS             2737..2970
                     /locus_tag="KLH32_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBE0140723.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3516..3983)
                     /locus_tag="KLH32_004123"
     CDS             complement(3516..3983)
                     /locus_tag="KLH32_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140724.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          995 a          935 c         1190 g          979 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacagc ttgtctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc ggtttaccat gccggagaga taaaaggccg tcaggatgtg attcaggctc
     2101 tcacagaggc agggattgac gtggttaggg tgacgcgaag cagtatcagc attgcagacc
     2161 cgaacggcgg gaagaatatc agactgaaag gagcgtttta tgagcaatct tttacagacg
     2221 ggtgcggagt tcgagaaaaa gctgaaagag agagccgaat ctacagagaa aatgctgaac
     2281 gacgagttca ggaagctcga agaatctgta aacagggaac tgacctcaaa cgaatcttta
     2341 atcaggaacg ctatcaacga ccatacatca gccctgaaag aacttctgga gaggtatcag
     2401 aaaacgaccg tcgatacgat ggacgcacac tggaaaaccg tactgaaaat gagcgtgaaa
     2461 cgctggctgt ggctgattat cgtctcagta ctgatgtttg ccacgaccgg gagcctgctg
     2521 tggtaccagg gcatgaagat aaacgcgaac atgaatattc tccgcgagta gaaagagagt
     2581 ctggagaagc tgaacgcgaa gacatggggc gtgcgttatc acgaggacag caacggacgt
     2641 tttctggtgt tgcccgaggg gatgaaagcc gaaacgaact ggacgatgga aaacgggaaa
     2701 cagaatgccg taaggctggt acaggagtaa cagagcatga cggagctgga aagacatttg
     2761 ctgaacgcat tagaacaact gcaacaggac tatatgcagc ggctgaacga atgggagagc
     2821 gccttcgtgg aattgcagaa aatgttttcg cttacgcaac gggacaacgc gatgctgaac
     2881 gagcgggtca tgcagttgag tcagcaggtg cagcacttga gcgagcagac agaacgcttg
     2941 agccagttat acagcgagaa caggagataa gggacgaacg gctgatacag gaatgcgaac
     3001 atgcgttatc cctggagcgt gagcaccagc tggaaataca ggaacggaca ctggatggcc
     3061 cttcgctggg atggtaatca gagacaggct aaaatgttgt cagcgcgttt ccggtgcatt
     3121 ttttgggata atggttggtt cgggggcacc gggaacgcgc ttcagtcaga ccttgctgaa
     3181 aacataggaa cgcgttaaaa cgcatacggg cgcgattctg gtgtgtcttt tgttgctgtg
     3241 ttttgcctct attgccagat cgcgctcatt cccctgaact tcttttttcc cgctcaggtc
     3301 cccgtcttct tcgcttccga aagcatgaaa aaaatccgtt tcagctgtct ctggctggca
     3361 attcctgcgt gattcacatg gctgcatagc tatgcatgtg gttaaaattt accgggtgcg
     3421 atcgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg
     3481 ccgaacgcgt ttcagcggtg cgtacaatta agatattacg ttaaattgtg agggaatggt
     3541 ccccatatac tgactttatc atagggatag ggttggtagt gggtcgattg ggtttcggca
     3601 ttgtttccgt taatgccaat tgcatttttg tatttgatgc aacagaaggc gtgagattct
     3661 cctgggcctg gaaaggataa gtgtttagaa ttgatggcaa aatatcttcc atccgatttt
     3721 tgaagtagtt gttgtttggc tgtctcccaa agggtgttgt tacaaacttc ttgaatttgt
     3781 agtttttgca ggactcttat tactgcgtta tcatgttcca attctttagg ggagtgtatt
     3841 attctctctt ttaataagct gtttaccaca gggtttagtt gttgtctaaa gcacgcagat
     3901 aatgccattg ctacacaatt ttgttcccga ggaagcatgt ttggcggggt atgccgtgtt
     3961 tgatgtgata tgtctattct catagaaggt ctctggctta tagcatcaac aaatacggct
     4021 ataaaacaaa attttcatag agtgattcaa gatcttcttg agatcctttt ttgcggcgcg
     4081 taatctgctg cctgcaaac
//
LOCUS       DAEEDS010000199         3935 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEEDS010000199 DAEEDS010000000
VERSION     DAEEDS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.203"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..380
                     /locus_tag="KLH32_004124"
     CDS             <1..380
                     /locus_tag="KLH32_004124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBE0140725.1"
                     /translation="LPASRVYPGSSRQAYLANRALHELRKNRTLPVDEAWVDDFVREA
                     FGRMKKEYVCKDSEMATEGATDLWEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQ
                     NAENVSEYLSLRHQGLQQSIQSL"
     gene            438..1412
                     /locus_tag="KLH32_004125"
     CDS             438..1412
                     /locus_tag="KLH32_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140726.1"
                     /translation="MTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSGKRKEQQ
                     MLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALELMTQIE
                     STVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKIS
                     FPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGWKEYSVD
                     VSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREKVEQLRN
                     TLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            1409..2314
                     /locus_tag="KLH32_004126"
     CDS             1409..2314
                     /locus_tag="KLH32_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBE0140727.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2311..3381
                     /locus_tag="KLH32_004127"
     CDS             2311..3381
                     /locus_tag="KLH32_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBE0140728.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
     gene            3596..3935
                     /locus_tag="KLH32_004128"
                     /pseudo
     CDS             3596..3935
                     /locus_tag="KLH32_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          985 a          943 c         1126 g          881 t
ORIGIN      
        1 tgctgcccgc ctcccgcgtt tatccaggct catcacgtca ggcgtacctg gctaaccgtg
       61 cgcttcatga gttacggaaa aacagaaccc ttcctgttga tgaagcctgg gtcgatgatt
      121 ttgtcaggga agccttcggt cgcatgaaaa aagaatacgt ctgtaaagac agtgaaatgg
      181 caaccgaagg ggcgacagac ctgtgggaag gctcacttat cgatcaactg ataacggaag
      241 tcatacagag ctcccattcc agagcggcgg cactggcggt tgattctgcc gccgcaaaac
      301 tgatgcagaa tgcggaaaat gtcagcgaat atctgtcatt acgccatcag gggctacagc
      361 aaagcattca gtcactgtag tcgcatatca ccagcctgct tgcggatatc cgggaaatcg
      421 cggactgtca ggagcaggtg accgctgatg tcagaatggc catggaggag atcgatgcca
      481 gaacccggga attactgacg ggggtctgca cctcactgga agaggagctg aatgactatt
      541 tcagaagcgg taaacgcaaa gaacagcaaa tgctggagga agaaaattca gcgcagcccc
      601 gggagagaaa cgcattcgct ttctttcacg atattttcgg aaccggaaat cagcatgaca
      661 ggatgcggga ctttgatccg gacagccccg aaataaaatt cagtgatcgc agggaagccc
      721 ttgaactgat gacgcaaatc gaatcgaccg tgaccagcct gcaccgtgag gctgaagcac
      781 agttccgtcc tgaactggaa aaaatcgtca ggggtattga aacaggtttt cgtggtacgg
      841 ccctgtacgc cacagaaaat attgccggac gcatcaatgc ccgtctggag gatgaaggtt
      901 tcactgtaaa aatcagtttt ccggcagtca gccagttaca gacccggctc gcagtaaaaa
      961 caaatctgag cgcgcttatg gaggaaagaa cggagacagt aacccgtcgc tgtcgacagg
     1021 atggtgtctg gggtacgctt tgtcgatggg caaacaccag tgactggggc tggaaagagt
     1081 acagtgtgga tgtgagccgc agcgtgatca atatcaacaa ggtcagagag gaagtgatgt
     1141 cactgacccg ggcatatttc ggggagctgc aggcatccat tgagcaggat attaaccagc
     1201 ccgtccgcca ggaaatcgat gcctttttct gtacattcag ggagaaagtt gaacagctgc
     1261 gtaacacgct gattcagagc tctgaagatc ataaacgcga tcagcaggcg caggaacggc
     1321 tgaccgggcg tcttcaggcg ttaaatgaaa gggttcctga gctcattact gacagtaagg
     1381 cgttaaggga agaactggag acaatgctgt gacttcacca tttattcaac aaattgctga
     1441 taaccgggta tgtcaggtac tcacctgtct tcctgaaaaa tttgtggttg atttcgcaaa
     1501 cggtattgat gttgcacagg agcatatccg tacggccgga gagcgcacgt tcttccggcg
     1561 gttaaaagag gggctgagcg gcgaaggtac tgcacggcag aatgccatca atgcttcgct
     1621 tgcacagggc gttgaggcgt ccctgcgctg gctgacggag ctgacaacgt cacttgccac
     1681 cacaaactac gcgattaccc gggtaaacga cagggtcagt tcactggtca gtgatactgc
     1741 caggctggcg cattattccg cagatacgcg ggagcagtta ctcaccctgg ccgatcaggt
     1801 tcaccagaaa ctgaatcatc ttgaagaaaa actccaccgt gttgacctgg ttcagcgggc
     1861 gcagttacat cttgagcaga tattctcctg gtggagcgcc gggcgatatg cgtcattttc
     1921 ccctgccgga cgttgttatg tggcacttga agagcttcgc tggggcgcgt ttggtgatgt
     1981 gatacgtcag ggcgaaacag gccaggttaa ccagctactg gatattctca gacataaagc
     2041 attaacgcag atggcacagg agagtggcgg tagtgcaacc gtgcgtctga acactctgga
     2101 ctggcttggt ggtcagggcc gggagcaggc agataacgag tggcatgacg cgattaactg
     2161 gctgggggac tggtgcagtg aagagcagca tccggtgatc tggtcaacca cgcaggctgc
     2221 agaacactta ccggttcgca tgccccgtct ctgctctgca gaacgcctct ctgaaagtat
     2281 ggtcgatgaa atatttcaga agggggctgc atgagtatag aaataaaaac agggctggtg
     2341 ctgtccggcg gtggggcggt gggcgcttat caggcgggag tggttaaggc actggcagag
     2401 tgtggtacac agatcagcat ggtttcaggg gccagcattg gcgcattcaa tggtgccatt
     2461 atcgcggcct ctcccgatct gtcagaagct gccgtacgcc tggaggcgct ctgggatcat
     2521 cttgggaata atcaggtgct gtcggtaaac cgatcggttt acttttcatt gctgaaaaaa
     2581 ttgttccagg caatgaacct ctgccagatc cccggacgtg caggagcact gcttacgaca
     2641 cttcttcgcc atatatcgat aatcaacggc ttcgacaatc cgatgactca gccgttgttg
     2701 tcagatgggc ccctgacagc gttgatggat cattatcttg atacagatgc tctggcaggc
     2761 gggctaccgc tgtatgtgtc gctgtacccc acagaagggg gcatgcagga tattattgac
     2821 tgcattcgtg ctgaactggg ttccggaacc acgaaaaacg ccgtttttca gcatatccag
     2881 agtctgcccc gcggacagca gaaagaggct ctgcttgcgt cagccgcact gcccctgttg
     2941 ttccgtcccc gtgaggttca ggggacaatg ttcggtgatg gtggtatggg aggatggcga
     3001 aatatgcagg gaaatacccc tgtaacgcct ctggtcgatg ccggatgcaa tatggtgatt
     3061 gtgtcgcatc tgagtgacgg ttctttatgg gatcgccagg cttttccgga caccacaatc
     3121 cttgagatcc gtccccggaa aaggctgaaa tatgcaggtg atggtggcaa cagtggcggt
     3181 ctgctcagtt tcacgtcggc acataccgac gcctggcgtc agcagggcta cgaggacacg
     3241 atgctgacga tggagcatat ccggaaaccg ctggcagcac gtcaggcact gtcccggtca
     3301 gaaacggtgt tgcagaaaag tctggagata acggaagagg cagatttggc actgagaagc
     3361 gcgatggccc ggattaaata aagacgcccc aaagaaacgc caccgcctgt ggtggtgttt
     3421 ttcctgtctg gtgttgttga aaatattttc agatatatat tacctgggtg agatttaaga
     3481 ttaacgcatt tattaatgtc ataaggagag ttatcatgaa attcatcgta aaagcactta
     3541 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaagtg ctccagtggc
     3601 ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
     3661 aagcaccgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca
     3721 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
     3781 tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg
     3841 tcacttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc
     3901 atcgtctgcg cggaaatgga cgaacagtgg ggcta
//
LOCUS       DAEEDS010000200         3777 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEEDS010000200 DAEEDS010000000
VERSION     DAEEDS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.205"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            32..493
                     /locus_tag="KLH32_004129"
     CDS             32..493
                     /locus_tag="KLH32_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBE0140729.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="KLH32_004130"
     CDS             complement(534..1004)
                     /locus_tag="KLH32_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBE0140730.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KLH32_004131"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KLH32_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBE0140731.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KLH32_004132"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KLH32_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBE0140732.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          993 a          975 c          858 g          951 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag atgtaatgcc
     3601 gatcagttaa ggatcagttg accgatccag tggctgtgta agaatccgga aacgctcact
     3661 tgtttccgga tttttttatg cacattggac aggctcttga tctggtatcc cgttacgatt
     3721 ctctgcgtaa cccactgact tctctggggg attacctcga ccccgaactc atctctc
//
LOCUS       DAEEDS010000201         3713 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEEDS010000201 DAEEDS010000000
VERSION     DAEEDS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.206"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="KLH32_004133"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="KLH32_004133"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBE0140733.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="KLH32_004134"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="KLH32_004134"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBE0140734.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="KLH32_004135"
     tRNA            2657..2732
                     /locus_tag="KLH32_004135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            complement(2747..>3713)
                     /locus_tag="KLH32_004136"
     CDS             complement(2747..>3713)
                     /locus_tag="KLH32_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0140735.1"
                     /translation="LEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKM
                     TNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLD
                     KAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQ
                     IDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTK
                     APTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWES
                     IEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          853 a          933 c          993 g          934 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac cattaaaatc aataagttac acatcattag
     2761 taccttcctt attttttgac tgggacaaat ttgggaccga tgggttcagg atcgagtcta
     2821 tttgccgtgc gtgttcggta aggtgattag gtgcaaggtg agcatatcga cgaaccattt
     2881 cgatagactc ccagcctccc atttcctgta acactgacaa cgggactccg gcttgaacca
     2941 gccaacttgc ccaggtgtgt ctcaagtcgt gaaatctgaa atcatcaata ccagcccgtc
     3001 tcagcgccgc tttccaggct gtgtttgcgt cataccgcat cttccttact gttggcgctt
     3061 tcgttccgtc tggtttggta cagctttcct tgtacacaaa tacccaacgg tgatgattcc
     3121 cgatttgttt tttcaatacg cgacatgcag tatcattcag cgcaacgcca attgcgcggt
     3181 ttgatttact ctcttccggg tttatccatg ccacccggcg ctgcatatct atttgttgcc
     3241 attcaaggtt gatgatgttc gagcgtctta agcctgttgc cagtgcaaat tcaacaacag
     3301 actttaatgg ctccggacat tcatcaatca gcctttgtgc ttcatggggc tccagccagc
     3361 ggatccgttt attctttggt tgaggcactt taataattgg tgccttatcc agcattttcc
     3421 attcacgctc tgcggctctt agtagggcct ttataaatga aagatgcgta gccttcgttg
     3481 caacggacgc tggttttggc gtgtattctg gaacaggttt cccttttttt ctgcatgctt
     3541 ctgccctgag tttccagttt tcctcatgac gccggttcgt cattttctgc attgctgaat
     3601 aaatttttga ttcagtgatg tctcttagtt gcatccctgc gaaatgttga agccagaatc
     3661 cgatccggct tttgtcatcg tccagtgatt tcttatgtgc tttctcttcg agc
//
LOCUS       DAEEDS010000202         3666 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEEDS010000202 DAEEDS010000000
VERSION     DAEEDS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.207"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            225..1453
                     /locus_tag="KLH32_004137"
                     /pseudo
     CDS             225..1453
                     /locus_tag="KLH32_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1828..2016
                     /locus_tag="KLH32_004138"
     CDS             1828..2016
                     /locus_tag="KLH32_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE0140736.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2066..2395)
                     /locus_tag="KLH32_004139"
     CDS             complement(2066..2395)
                     /locus_tag="KLH32_004139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140737.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(2520..2699)
                     /locus_tag="KLH32_004140"
     CDS             complement(2520..2699)
                     /locus_tag="KLH32_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140738.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            2829..3029
                     /locus_tag="KLH32_004141"
     CDS             2829..3029
                     /locus_tag="KLH32_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBE0140739.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            3019..3240
                     /locus_tag="KLH32_004142"
     CDS             3019..3240
                     /locus_tag="KLH32_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140740.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            3233..3453
                     /locus_tag="KLH32_004143"
                     /pseudo
     CDS             3233..3453
                     /locus_tag="KLH32_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3455..>3666
                     /locus_tag="KLH32_004144"
     CDS             3455..>3666
                     /locus_tag="KLH32_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140741.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRY"
BASE COUNT         1024 a          829 c          890 g          923 t
ORIGIN      
        1 tgcaaccgat tataacggat gcttaacgta atgcgtgaag tatggacata ttttatcaat
       61 tagtgttttt tgccgtttca taatgtttca gattgttcat tatgtttttg attataaaca
      121 gatttgtgat tttccttgtt ccagcttgtt tcacgttgta gccctttgtg caacgatcgg
      181 tgtagttaat ggtgtagtta attgtgatga ccggaatcaa aattatgagc agagcactta
      241 acaaactgag cgatacacag ctgaggaaaa tcaacggcac acccgcccaa aaaacagcct
      301 ttcttaatga cggtggaaac ctgagcgtca ggcattcaac cagtggcctt ttaacctggt
      361 atttcactta cagggccgga acgggaaggg gggcaccacc ggaacgcatt aagctgggaa
      421 attatcctga tctgaacctg aaatcagcca gggaaaaagc cgcccagtgt cgcgcatggc
      481 tggcagaggg gaaaatccac gtcatgagct taattacacc gtacaggaag cgttaaagcc
      541 ggtaacggtt ggcgatgcgc tcacctactg gcttgagtcg tacgcaaagg aaaaccgcgt
      601 ggattatgcc gccctgaaaa agcgccttaa taatcacgta atacagcaca ttggtgctat
      661 gccgctggat aaatgcgagc tacggcactg gctggcctgt tttgaccagg tggcaaagcg
      721 aacgcctgtt actgccggat tcttgctaca gacgtgcaaa caggcgctta agttctgccg
      781 gaggcggcgc tatgcaatca gcaacgttct tgatgatatg agtgtggcgg acgttgggaa
      841 aaaaccggat ataagcgagc gtgtcttaag caccaaagaa ctgggcgaat tattgcaggc
      901 actggacaaa aaaatattct ccccctacta catcgcgtta atccgcctcc tgattgtgtt
      961 cggatgccgg acggtagaac tgaggttatc ggagatcagc gagtgggatt ttaccgaaat
     1021 gctctggacc gtgccgaaag aacacagcaa aacgaaggtg gcaatattcc ggcccatacc
     1081 ggaagcaata ctgccgttcg tcacgcagct ggtggagcag aacaggcaca cgggcttatt
     1141 gctgggggaa gtgaaacagg aaacaagcgt gtcgcagtac ggcagattag cgcacaggag
     1201 gctaaatcac cctcactggt cactacatga catccggcgc acctttacaa cgatgctgaa
     1261 cgatttaggc gtggatcctc acgtcgtgga gcagcttaca ggccaccaga tgccaggaat
     1321 gcagcgagtt tataatcatt cccgttatct ggatgctaaa cgcaatgcgc tggatatgtg
     1381 gacggagcgg ttagggatac tggcgggaac acatgaaaac gtaaccacgc taccagtagc
     1441 cagaagaaaa taattttttt cgtgtttttt cagtatgcgc atactggata tgcgaacaga
     1501 tacaacgtgc aacaatgagc aacaaagcgg aacaactacg aacaagaggt gaaaaagtgt
     1561 acagatttat aaatagctga tttttaatgt gttgctgatt ttttatacac tagcgaatca
     1621 ctctttaaat agcgagaaaa aaggcatgaa acaatatttt atgcctttgt attaacatga
     1681 atttaaagga ttttatcctg attattcctc tttctgtaaa ccatgcggcc tcaacatttg
     1741 ccaactctga atcatcctga cacaatcaga cacatcccaa cgcaatagaa cacgaaataa
     1801 ctctttaaga aacgaaaggg ggcaatagtg ttaagcactg atcggtttat acgtgaaaaa
     1861 gaatgcgaaa agctaacagg ccttagccgc tcatgccgct accgcctgga aaaggccgga
     1921 caattcccat cacgtcgtaa acttggcggt cgttccgttg gctggtcttt atccgaggtt
     1981 ctggcctgga aggatagctg caaggcagtt cattaatcac gctggcggca cgcagccgcc
     2041 acacatcaat catctgaaca cagagctata accatgaaga ttgaatatac gccagaacgt
     2101 gggcggggat tcgttcgccc tggtgaaact ggaaaaccac aaaattgggg tttttcaggc
     2161 ataaaaaaag cggccccgaa atggagccgc ctttctgaac agataacccg ctgcgccgtt
     2221 tgtgtgtgtg atcccaaaca taagcacggg gatgatagcc gctatcaggc tggtgggcaa
     2281 tgcaatcagt ctggttcagt gcgttgccat aactgcgaag ttctctcctt tgatgagtat
     2341 tctttaaggt acttctcaag ggcaaaagaa cacggcgcga atcgttctga ttcatgctct
     2401 ttctttctgc gtcgtctttt tcgtgcaggt gatgtttttt cggttgattc ttcgttggtc
     2461 attgtgtgcg cctgtaaagc aatgcgccgt agtacctcac accacggcgc tgatagtttt
     2521 tatcctttgg gttctatgcc gcgttgctgt aattctttgc ggattattcg ctttatccag
     2581 gcagcaagag aatcatcgcc gtcctgttgt tgtgcctttt ccatcttttc ccgtaagtca
     2641 ggatctaaac ggaactggaa cggaggatta ccacgccttt cgtttttgtg tgttgacacg
     2701 ttaattacac ctcaggtaat gtatttatgt gtagtgacac attacacaca atcaacacaa
     2761 aaagcaaagc cccgcactgt aggagcagta acggggcttc taaccaacaa cgtaaactag
     2821 gagccgttat ggttgccgta aatcatatac cacaccttgt acacacacaa acggcctttg
     2881 tgtggcgttt tctggcactg agtgccggag aatcccaaat catccacgta accgcttgga
     2941 cggaacgcga agcgcgtagc cgttgcccgt ccggttgtgt tgctgtattc gccgcccgta
     3001 ttcgtcaggg ggaaacctat gcacaataaa accacaccga acgcagcagc cgccgcactc
     3061 actacgctga tgcacgcgct tattgatatt gaatgcacgg cagagcttgc gcagggagaa
     3121 gaacagaaag accggacaca gttcgccctg gaatgtatcc gatacatcgc aacgcggtca
     3181 ctgaatgacg ctaaaaatat tcttgttgcc gattgtgaaa atgggggggg ttatgcgtga
     3241 tgatcgtttt aattccctga aacaggaatt ttccggcgtt cctgatgatg cggctgatgc
     3301 gctttcgtca atgccagaac ttattagagc ggcttttttc ttactttcca cgagagaata
     3361 taaatcaacg ggcttgatgt actgaatatc gccgccgatt atgctgattt cgtgacagag
     3421 gttattttaa gaaaaacgac ggacggggat taatatgcgt gatatttacc acgaaacaat
     3481 agaccgcgca tttcttgcac tttctcacag tgaaaacatg ctggaaatat tgcgcatatg
     3541 gcttgaaaca cttggcgaca atgaacgcga caaacaaaaa tcaagaattg ccacggcatt
     3601 aataacgctt cttgagcctg taataatgga actgcaagaa atagatctat tgcacgacag
     3661 atataa
//
LOCUS       DAEEDS010000203         3548 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEEDS010000203 DAEEDS010000000
VERSION     DAEEDS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.208"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="KLH32_004145"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="KLH32_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBE0140742.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="KLH32_004146"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="KLH32_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBE0140743.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KLH32_004147"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KLH32_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBE0140744.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAEEDS010000204         3288 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEEDS010000204 DAEEDS010000000
VERSION     DAEEDS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.210"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="KLH32_004148"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="KLH32_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBE0140745.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="KLH32_004149"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="KLH32_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBE0140746.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..3070)
                     /locus_tag="KLH32_004150"
                     /pseudo
     CDS             complement(2711..3070)
                     /locus_tag="KLH32_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(3086..>3288)
                     /locus_tag="KLH32_004151"
     CDS             complement(3086..>3288)
                     /locus_tag="KLH32_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140747.1"
                     /translation="GWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLS
                     FSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          770 a          735 c          802 g          981 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gataattttc ggtaatgact ccaacttact gatagtgttt tatgttcaga taatgcccga
     3121 tgaccttgtc atgcagctcc accgattttg agaacgacag tgacttccgt cccagccttg
     3181 ccagatgttg tctcagattc agattatgtc gctcaatgcg ctgagtgtaa cgcttgctga
     3241 taacgtgcag ctttcccttc aggcgtgatt catacagcgg ccagccat
//
LOCUS       DAEEDS010000205         3230 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEEDS010000205 DAEEDS010000000
VERSION     DAEEDS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.211"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(438..1022)
                     /locus_tag="KLH32_004152"
     CDS             complement(438..1022)
                     /locus_tag="KLH32_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0140748.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(1022..2260)
                     /locus_tag="KLH32_004153"
     CDS             complement(1022..2260)
                     /locus_tag="KLH32_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0140749.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(2257..3162)
                     /locus_tag="KLH32_004154"
     CDS             complement(2257..3162)
                     /locus_tag="KLH32_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBE0140750.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          661 a         1037 c         1001 g          531 t
ORIGIN      
        1 gccgtgctag tctgctcggt gatggtggag tgaagccaac ccgcaatcgg gttatgaatc
       61 tgcatcgcga ttcgcaatca gctgtctctt gagcatgtcg aactcctgcg atgttatctc
      121 gccgtgctcg tgctgctaca ccaacttgtc caactcatcc gccacaccgc tccccaccac
      181 ctgcgatggc caggttgttt gctgggcgca ttggcggagt atcgtctcga cccgggacac
      241 ccacaccggc acaaccttac gggagtgatt cactgtcaaa gaatcggccc ggtgctctga
      301 cgcaagtatc gggatggtca ccatttgtaa gccgtagacc tgagtggtga tcaagacttc
      361 gataccaccg accgtaccgg tactaatcga cgacggtcgt gttcgtcgcc tgccgcaggg
      421 actctgcaca cctccgttta cgcatgtgcc tggaggagtt ggaaatcgtc gtgttcggga
      481 aacattaaac acaggatggc agcgatctga gccagcacat gatcagctag ctcaccatcc
      541 ggatcgacgg cccactgcat cgtcgcgcca gcgatgaccg agtgcaggag caactcagct
      601 gccgcaggag cacctggggg cagtcgcttg cggatcccct ccaccaccgc gcggttccgc
      661 tggatcgcaa gcgtgcgtag ctccggcacc tggagctcgt accaggagat gagatagttc
      721 accgagaagt cgttgcgagt gttcatgctc cgaacgagca cctgcaaaaa ttcccagagc
      781 ccttgcggcc ctgcgcctat cggtatcgca ttcaggtaat gccgcacctg ctcgacgccg
      841 cgctccatca tcctcaccag cagcgtatcg cggttggtga agcgctggat taacgctgcg
      901 cgggagagcc ccacctcctt tgctactccg ctgagcgtga actctatggg accgcaacgc
      961 ttcagcacta cggtggcggc ctcgagtacc tcgtcatcgg acttgagctt ggggcggggc
     1021 atcagtgttc accttctgta tgggttgggg cggaggctgt ggctgccgcc gccattgtag
     1081 caaattgaag acggagcgag agtagagcca cgagccccgc aaacacggcc gatacaacga
     1141 ggccagggag cggaccagca aggtcgacaa acgcgccggc cgcaagcata accatgggcg
     1201 aggctgacag catcaccgcc gagaccgtgc cgagtacccg gccgagaagt tctggcggcg
     1261 tgcggttgta gatggcagcg ttgagaatgg gagagactga gccggtcagc agtcccacga
     1321 gcgcgcccaa caacatcagc accggcacgc ctggcaactg tgaaagcaga agcgagccca
     1381 ccgcagagcc acaaaatgcc accgccagcc agttctgcgc tgatatccgg gcgccgaccg
     1441 acgcatgaat ggcaatgcca aggagaccac cagcccccat cattgaggag aacagcccga
     1501 gctctgctac ttggcgtcct gcatctacaa acagcgcagg catgatgacg ctgccgttgg
     1561 cgccaacgat gcccacgaag atcatcacta taccaaagag agggcgcagc aggggttcgc
     1621 tccagagaaa agcgacgccg gcgcgcatgg agagagtcgc cgtcgtggtc atcgtccgag
     1681 cggcacgcgc gggaagcacc cacgcgccga gcagacctgc aaggacggag cagaacgccg
     1741 tcagcccgag cgttggcgca gcgccaagca ggccgattgc ggccccccca agggccgggc
     1801 cacctagaat cgcgacgttc ccgatcaccg ctttcagtga cgagacgcgc tcaacggaga
     1861 gcccggcgac gtggccgagt ttgggcagct cactgtcctg cgcggccata ccgggtgcgt
     1921 cgaacgcggc accgagcacc acgcaagcga tcagcccagt gttcgagagg gcgccaacgg
     1981 catcgagcag tgggatgctc gccatggcca cgccgcccac cacacccgag atcaatgcga
     2041 cgggcgcgcg cccgaaccga tcgacgaggc caccaccaac ccacgcgccg atgatggtcg
     2101 cgatgacgct gctagcggcc gtggcgcccg cccaggccgc gctctttgta tgagacagga
     2161 cgaaccatgg aagcgcgagg gccgccaccg cgttgccgat ccggaagaga aaggtcgccg
     2221 cgaacagcgt cgcgagcggg ctatatcgac gttcgctcat tccgctgcgg cgagctgcgc
     2281 cttcgccgca gcgaggtact cttcgttacc cgagtcgagg gcgaagagtg cgtaggtgac
     2341 cgccccgaac gcaaggcgct ccgcgatgtg gtgggcgagc cgcggccaca cccggccacc
     2401 ggccgcttca tacgtgagga ggagcttcgc gagcccctct tcaccaaaga ccataaggtg
     2461 cgcggccatg tcgatggcag ggtcatcaac gcgggcctcg ctccagtcga tcatcccgct
     2521 gacgcgctcc gtgttgtcga tgagcacatg gcccacgtag agatcgccat gcaccaccac
     2581 ggagaaatct ggccacgacg aatcgtcgtc gagccagcgc tgccaccggt ggaggcgctt
     2641 gtcgttcacc acgaactcgc gtcggacgcg gtcaacgtcg tcggccacct tctgacgggc
     2701 ctgcgtcggt gtacggatga gcatccccgc atccacggcg gcggaaatgg ggacggcatg
     2761 cagggcggcg agcgcggtcg cgaagctctc cgcgaagacc tccgagtcct gcggcacgac
     2821 ccagtcgggc gtggacgaac caggctggat gaccatcgca gtcgagtctt cgagcatggg
     2881 ataggcaacg agctcggcgt tggccacgcg ccagtccggc accgcgaacg gcaggcgatt
     2941 cttgagcatt gccagcaccc gcgcctctgg ttcgaccttc gcgcttacct cggctcggcg
     3001 cgggatgcgc agcacccacc gacgtccatc gtcgacggtg gcgatcacga tcctatagtc
     3061 gagcccaagc tcattgacag tcagcgggcc atggagcttg agcccatgtc gggctgcaag
     3121 tgcgtacagt tgggaggtat cggcggtcgt gactacggtc atgattcact cctgagggct
     3181 tgacgggttt agccacctaa atgtaacagt cacgtcggtt atattcaatc
//
LOCUS       DAEEDS010000206         3179 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEEDS010000206 DAEEDS010000000
VERSION     DAEEDS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.212"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            417..1526
                     /gene="fepE"
                     /locus_tag="KLH32_004155"
     CDS             417..1526
                     /gene="fepE"
                     /locus_tag="KLH32_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBE0140751.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2551..2745)
                     /locus_tag="KLH32_004156"
                     /pseudo
     CDS             complement(2551..2745)
                     /locus_tag="KLH32_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          800 a          658 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgtccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtggtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgatggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataattc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAEEDS010000207         3108 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEEDS010000207 DAEEDS010000000
VERSION     DAEEDS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.213"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..195
                     /locus_tag="KLH32_004157"
     CDS             <1..195
                     /locus_tag="KLH32_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140752.1"
                     /translation="PLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFS
                     KSVELHDKVIGHYLNIKHYQ"
     gene            211..336
                     /locus_tag="KLH32_004158"
                     /pseudo
     CDS             211..336
                     /locus_tag="KLH32_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            369..827
                     /locus_tag="KLH32_004159"
                     /pseudo
     CDS             369..827
                     /locus_tag="KLH32_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1176..2348
                     /locus_tag="KLH32_004160"
     CDS             1176..2348
                     /locus_tag="KLH32_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE0140753.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2402..3025)
                     /locus_tag="KLH32_004161"
     CDS             complement(2402..3025)
                     /locus_tag="KLH32_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0140754.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          957 a          652 c          612 g          887 t
ORIGIN      
        1 ccgctgtatg aatcacgcct gaagggaaag ctgcacgtta tcagcaagcg ttacactcag
       61 cgcattgagc gacataatct gaatctgaga caacatctgg caaggctggg acggaagtca
      121 ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta tctgaacata
      181 aaacactatc agtaagttgg agtcattacc gatgaagcca tctcagtaat acgggaatac
      241 attgagattt tctacaatcg tcagcgtcgt cactctcgtc tggggaatat ctccccggca
      301 gccttcaggg aaaaatatca tcagatggct gcttaaaaaa gaacaaatgg tagtgtccgc
      361 tattgccagt acacctcaaa gagagtaaat caaaccgcgc aattggcgtt gcgctgaatg
      421 atactgcatg tcgcgtattg aaaaaacaaa tcggaaatca tcaccgttgg gtatttgtgt
      481 acaaggaaag ctgtaccaaa ccagacggaa cgaaagcgcc aacagtaagg aagatgcggt
      541 atgacgcaaa cacagcctgg aaagcggcgc tgagacgggc tggtattgat gatttcagat
      601 ttcacgactt gagacacacc tgggcaagtt ggctggttca agccggagtc ccgttatcag
      661 tgttacagga aatgggaggc tgggagtcta tcgaaatggt tcgtcgatat gctcacctcg
      721 cacctaatca ccttaccgaa cacgcacggc aaatagactt gattctgaac ccatcggtcc
      781 caaatttgtc ccagtcaaga aataaggaag gtactaatga tgtgtaactt attgatttaa
      841 atggtgccga taatagcagt cgaacctacg accttcgcat tacgaatctg tagcaccaat
      901 cataactatc tgttttagca agcattaacc gcattcacta agctatagtt gatgacacaa
      961 acagaaagtt gatgcatgat gttgccatat atatgtcaca aatacggcac aacgatcttc
     1021 aaacatatag ccacacattc acagaagagc acaaagcctt gcaatccagt gcaaagcttg
     1081 gtgtgtctcc gttttgtttc atcagttaca cataactata tgatcattaa ctaaaagtca
     1141 gtatacttgg agaaatttta attgcgtaat agagcatgca tatgattgaa attaacagtt
     1201 tattattaat aacatccgtg atattgatgt cgttattagc tgtggggtta tttgataaaa
     1261 tttccccaat aaatcttgtt gagcacggca ggaataatca aatagatgga atgcgggggt
     1321 tcttagcaat tttcgtgctt attcatcacg cagcaatttg gaatggatac ttgtcatctg
     1381 gagtatggga agcaccttca tcaaatctgt tagcaaattt aggccaagtt ggtgtgtcat
     1441 tcttttttat gattactggt tatctgttct tttcaaagat tatctctgga gatcaggact
     1501 ggacaaggct ttatgtatca agattactac gattaacccc aatgttcata gttagcctat
     1561 gtctcatttt tatcattgta ggttttaagt ctggatggag aatgcaggta tccacagaag
     1621 agctttttgt gtcaataatg aagtggttgc cattcactgc gctaggtatg ccgaacatta
     1681 atgacgtaaa agattcattt actatcaatg ccgctgtaac atggacactt gtatatgaat
     1741 ggttctttta tttttctctt ccggtaattt ccgcgctcat aaaaagaaaa gtcagtattt
     1801 acatggttat gattagcgca atatcgctat ttgttttcat tttatttttc agtaaaatac
     1861 acatagcatc atttcttttt gggctcctgg catttttgct aaataaatca aagatagtca
     1921 atggaattgc caaagcaaaa gtaacaccaa ttataataac tgcgataatg gtttttgaaa
     1981 tgacgtattt caaaacaact tacgcaccgc taccactgat tctttgtgga ataacattta
     2041 tcattatcgc atcaggttgt gatttgtatg gaatattaag attaaatata accagaaagc
     2101 taggagaaac tacctacagc gtttaccttc tgcatgggat attcctttat tgcctaatga
     2161 cgtggattat tcctaataat tacactgaaa atacatttat catactggta agcacaactg
     2221 catttcttat tacgttcaca tcatgcctaa cttttaaatt aatagagacg ccattcatca
     2281 aattaacaaa acaaaccaca actttagtaa aagaattaat acccacatta acaaacaaca
     2341 atcaataaac aataaacata agccgcccta catatatgtc caaatggggc ggcgaatgta
     2401 tttatattag tctggtaatg ctaaatgcaa acgaactgga gattcctgcc ccgctaagcg
     2461 tattgcgcag ggtcacccta aatgtatcta cgccaatata agtaacatca caactaactg
     2521 aatcaggcgc agatataggt gttatctgca caatataacc gctagcaact gctgcgagcg
     2581 gatgagaaaa gctaacatca tacacacctg tagaaaccac gttaaccgat gaaacaccag
     2641 tagcaacgtc gatactacca ttggcgttaa tttttcccga gcgtgtgact gacaatgcag
     2701 aagccgatgt taccttactg gttgtttctg taaaaacaca cccaatgtca actacctcgc
     2761 aattagctcc tgttatccag tatggtcgtt ctgcacttgg ctgataatcc cctgcgctct
     2821 ggaacgttgt gccgttgagt atgactgtaa cttttccccc acctgagctt gttacttgaa
     2881 tattcgtgtg cgtgtatctt gcactgctca cgcgatgaaa atttccaccg tttataacca
     2941 cagttacagg gcgcgtccca gtattatcta tagcaaggtc tgcccccccg gcattagcct
     3001 caaagtatgg gttgtttaat accaaagcgc acgaaccgtt aaggccattt atagctaatt
     3061 gcgcaccacc tgtactggaa tccccctgag taccatttcc ttcacaag
//
LOCUS       DAEEDS010000208         3097 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEEDS010000208 DAEEDS010000000
VERSION     DAEEDS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.214"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..367)
                     /locus_tag="KLH32_004162"
     CDS             complement(<1..367)
                     /locus_tag="KLH32_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0140755.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFE"
     gene            complement(406..693)
                     /locus_tag="KLH32_004163"
     CDS             complement(406..693)
                     /locus_tag="KLH32_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140756.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KLH32_004164"
     CDS             complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KLH32_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0140757.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1326..1991)
                     /locus_tag="KLH32_004165"
     CDS             complement(1326..1991)
                     /locus_tag="KLH32_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBE0140758.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2160..2516)
                     /locus_tag="KLH32_004166"
     CDS             complement(2160..2516)
                     /locus_tag="KLH32_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140759.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(2574..2996)
                     /locus_tag="KLH32_004167"
     CDS             complement(2574..2996)
                     /locus_tag="KLH32_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBE0140760.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            complement(3011..>3097)
                     /locus_tag="KLH32_004168"
     CDS             complement(3011..>3097)
                     /locus_tag="KLH32_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBE0140761.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
BASE COUNT          771 a          820 c          673 g          833 t
ORIGIN      
        1 cctcaaacga ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat
       61 attgctctgc cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt
      121 aatcaacgaa accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      181 tccagtaaac gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
      241 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc
      301 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
      361 gtgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
      421 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt
      481 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
      541 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
      601 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
      661 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
      721 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
      781 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
      841 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
      901 agaggtgggc ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta
      961 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
     1021 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
     1081 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
     1141 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
     1201 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
     1261 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
     1321 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
     1381 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     1441 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     1501 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
     1561 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     1621 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
     1681 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
     1741 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
     1801 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     1861 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
     1921 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
     1981 gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa
     2041 cggggctatc ggaaacagat gtgtgactgc tccccgccct ttcgggcggt ctcctgatga
     2101 tttgagggtg cagaaatccc tccggttaag gattaaattt ttaacagtgc taaatttaat
     2161 tattcagttc tggattttgt cgccctgcgt atccgcgctt tcgcgttacg ctcaatctgt
     2221 atcagctttt ctatatttct ccgcctttcc tgttcctcct ggcgcaatag ccttacatca
     2281 tctgccagtc tggtttctct ttttgccaca gagagcatcc agtcaaacgg ctccacaacc
     2341 gcaccgcaga ttttacagcg gacctgacgc tctttttcgt caacccgtac agaggcgtga
     2401 tggcaatatg gtctttccga tggcttataa agaaaattaa cctgattacg agggtcatcc
     2461 tcttttatcg gaaataaaac gatattgctt aactcatctt ctggttttat ttccatgctc
     2521 ctctcctttg atgcgaatgc cagcggtaat tgaagcctga tagctaattt cattcacagt
     2581 atcgcctcct gaaaattacc ctgatagaaa gccagtacac gctgcatagc ttcgctcttc
     2641 cggcactcgc gacagattat attcaggcgc ctgtcgtagc ggcgtatttc tccgtcaggt
     2701 aatgaccaga taaggtccgg atcaacaaca accggtttcc tcacctttac tctcgagagt
     2761 tttttgcggg cgctttgcca gtccttgcga gcctgttcag acgggaataa gccgtagcca
     2821 gaattatata catcaccact tgcgaccagt tctatgcata agcgaccgac agacgcacga
     2881 ctgatacctg tttcatcgga caactgccga atcgtgaccc gaccgtcctg atgcacgagt
     2941 tccacaattc gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt cgccataaat
     3001 cctcctgaaa ctacttaaca accctcgaat ggctaacatt cggacgccag ctctcccagt
     3061 taaaattaac ccagcgcccg ccgttcatga ccatgcg
//
LOCUS       DAEEDS010000209         2952 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEEDS010000209 DAEEDS010000000
VERSION     DAEEDS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.215"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="KLH32_004169"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="KLH32_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBE0140762.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="KLH32_004170"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="KLH32_004170"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBE0140763.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..>2952
                     /locus_tag="KLH32_004171"
     CDS             2604..>2952
                     /locus_tag="KLH32_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE0140764.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          659 a          702 c          779 g          812 t
ORIGIN      
        1 aaaggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg ggggcgaggt gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aa
//
LOCUS       DAEEDS010000210         2807 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEEDS010000210 DAEEDS010000000
VERSION     DAEEDS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.216"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            6..81
                     /locus_tag="KLH32_004172"
     tRNA            6..81
                     /locus_tag="KLH32_004172"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KLH32_004173"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KLH32_004173"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KLH32_004174"
     CDS             complement(299..1057)
                     /locus_tag="KLH32_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE0140765.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KLH32_004175"
     CDS             complement(1065..2168)
                     /locus_tag="KLH32_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE0140766.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="KLH32_004176"
     CDS             complement(2178..>2807)
                     /locus_tag="KLH32_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBE0140767.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          738 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAEEDS010000211         2717 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEEDS010000211 DAEEDS010000000
VERSION     DAEEDS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.217"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            6..224
                     /locus_tag="KLH32_004177"
     CDS             6..224
                     /locus_tag="KLH32_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBE0140768.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="KLH32_004178"
     CDS             226..591
                     /locus_tag="KLH32_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0140769.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="KLH32_004179"
     CDS             588..1253
                     /locus_tag="KLH32_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140770.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="KLH32_004180"
     CDS             1253..1618
                     /locus_tag="KLH32_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBE0140771.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="KLH32_004181"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="KLH32_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0140772.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          773 a          558 c          697 g          689 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgttagt
//
LOCUS       DAEEDS010000212         2690 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEEDS010000212 DAEEDS010000000
VERSION     DAEEDS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.218"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            230..769
                     /locus_tag="KLH32_004182"
     CDS             230..769
                     /locus_tag="KLH32_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBE0140773.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="KLH32_004183"
     CDS             complement(995..1318)
                     /locus_tag="KLH32_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBE0140774.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="KLH32_004184"
     CDS             1604..1798
                     /locus_tag="KLH32_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE0140775.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAEEDS010000213         2647 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEEDS010000213 DAEEDS010000000
VERSION     DAEEDS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.219"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..570
                     /locus_tag="KLH32_004185"
     CDS             <1..570
                     /locus_tag="KLH32_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE0140776.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="KLH32_004186"
     CDS             571..936
                     /locus_tag="KLH32_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0140777.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="KLH32_004187"
     CDS             933..1616
                     /locus_tag="KLH32_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBE0140778.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="KLH32_004188"
     tRNA            1815..1890
                     /locus_tag="KLH32_004188"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="KLH32_004189"
     tRNA            1892..1968
                     /locus_tag="KLH32_004189"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="KLH32_004190"
     tRNA            1971..2046
                     /locus_tag="KLH32_004190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="KLH32_004191"
     tRNA            2050..2124
                     /locus_tag="KLH32_004191"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="KLH32_004192"
     CDS             2417..2632
                     /locus_tag="KLH32_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0140779.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          692 a          582 c          715 g          658 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagtcaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcattac
//
LOCUS       DAEEDS010000214         2568 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEEDS010000214 DAEEDS010000000
VERSION     DAEEDS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.220"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..489
                     /gene="capU"
                     /locus_tag="KLH32_004193"
     CDS             <1..489
                     /gene="capU"
                     /locus_tag="KLH32_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBE0140780.1"
                     /translation="EFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHD
                     DVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQN
                     NQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAK
                     QK"
     gene            494..1444
                     /gene="virK"
                     /locus_tag="KLH32_004194"
     CDS             494..1444
                     /gene="virK"
                     /locus_tag="KLH32_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBE0140781.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1509..2453
                     /gene="lpxM"
                     /locus_tag="KLH32_004195"
                     /gene_synonym="msbB"
     CDS             1509..2453
                     /gene="lpxM"
                     /locus_tag="KLH32_004195"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE0140782.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          742 a          489 c          582 g          755 t
ORIGIN      
        1 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
       61 attgtggggt ctggttcacc tgaactgcgg gaacatttac agtatcagat tgacagtatg
      121 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
      181 gttgccagtc tggtggttct gccttcagaa aacgaatctt ttggtatggt gctggcagaa
      241 gcatcggcat tttctgtgcc tgtagtggcc actcagattg gtggaatccc tgaggttatt
      301 cagaacaacc agaccgggac attgttacca gcaagtaata agcacgcatg gatgtgcgcc
      361 ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcct ggcaaaacag
      421 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
      481 caaaagtaac aatatgtttt ctataagtaa cttatcattt atcggtttcc ttaaaaggat
      541 tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc ggttcatgta
      601 cattttgcga tgttctataa atccggttgt cagtattcga tattattatg aactgcgttc
      661 cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa gaattcatcg
      721 cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc ttgagcatta
      781 tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac aaaaatcggt
      841 atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt gttctcccag
      901 tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa cagttattgc
      961 ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta ttggcggatt
     1021 acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     1081 tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgcaa
     1141 tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     1201 gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg
     1261 aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc cgctaagcga
     1321 tatagccagt aaaaaacgct ctgagtaccg aaaacgatac gctttgctgg attatatcca
     1381 tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc agaatttaaa
     1441 ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag aggcattcgc
     1501 actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa aattaccttt
     1561 cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt tcaatgtttc
     1621 ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc agattgagca
     1681 aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa aagagtgata
     1741 cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc atagtgatga
     1801 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     1861 tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg gttccccatg
     1921 gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa atggccgcca
     1981 tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta cggcgtaaat
     2041 tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag tcagtacgcc
     2101 agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac agtgaatttg
     2161 ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg atgaacatca
     2221 gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat ctactgacta
     2281 ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa acgattgccc
     2341 gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag tatgtctggg
     2401 ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct tgaaattata
     2461 aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat
     2521 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaacca
//
LOCUS       DAEEDS010000215         2484 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEEDS010000215 DAEEDS010000000
VERSION     DAEEDS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.221"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..402
                     /locus_tag="KLH32_004196"
     CDS             <1..402
                     /locus_tag="KLH32_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140783.1"
                     /translation="SRAVIGWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(506..973)
                     /locus_tag="KLH32_004197"
     CDS             complement(506..973)
                     /locus_tag="KLH32_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE0140784.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(1039..1278)
                     /locus_tag="KLH32_004198"
     CDS             complement(1039..1278)
                     /locus_tag="KLH32_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBE0140785.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1413..2018)
                     /locus_tag="KLH32_004199"
     CDS             complement(1413..2018)
                     /locus_tag="KLH32_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0140786.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(2133..>2484)
                     /locus_tag="KLH32_004200"
     CDS             complement(2133..>2484)
                     /locus_tag="KLH32_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140787.1"
                     /translation="AKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGCPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKLVELH
                     DKVIGHYLNIKHYQ"
BASE COUNT          737 a          575 c          503 g          669 t
ORIGIN      
        1 tcacgtgccg ttattggctg gtcaatgtcg ccacgcatga cggcgcaact gccctgcgat
       61 gccctgcaga tggcgctgtg gcggcgtaag aggccccgga acgttatcgt tcacacggac
      121 cgtggaggcc agtactgttc agcagattat caggcgcaac tgaaacggca taatctgcgt
      181 ggaagtatga gcgcaaaagg ttgctgctac gataatgcct gcgtggaaag cttctttcat
      241 tcgctgaaag tggaatgtat ccatggagaa cactttatca gccgggaaat aatgcgggca
      301 acggtgttta attatatcga atgtgattac aatcggtggc ggcggcacag ttggtgtggc
      361 ggcctcagtc cggaacaatt tgaaaaccag aaccttgctt aggcctgtgt ccatattacg
      421 tgggtaggat cacaagacct actgctatca caattactga gagaaaaaaa agcatccgat
      481 gattcattta tgctgtccta aaaaattaac aaaacaatgg gaaaacagga tatgtaagca
      541 gaaaagccaa tgaaattggt acagcataag gcacaccgtt ttcacgaatt gattttcgaa
      601 aaaaaagaag acctgccaga acaaccagag cgccgcaaaa ggtcattgcg acgagaaaat
      661 tagccagttc atgttcatca atagctaacg aaagaacagt taacagcttg acatcaccgc
      721 cgccaattat ccctgcggaa aaaagaaaga agcccacgag caaaacgctc ccggctgcaa
      781 ccagattggt tttttgatgc tgtaaaatga gtgccaaaaa aataagcacg gttaacacca
      841 gtaaataacg atggctaata attctgagta atatatcccc ccatgaaata aaaataagaa
      901 agagcgcaac aaataaaaga tcgactctta gtaaccatat tgctgccatt gaaagactcc
      961 atgtaccata caaggttgct gccagacata ttcctttacg tctgacagcc tctagagagt
     1021 ttttttattt tttataagtt acggattact cgttccggaa atcgttacct gttgaattgc
     1081 tgcagcgata gcgtcgaatg catctttaat tgcgcccagg aagcctgagt tctgatcgcc
     1141 aaagatgaaa gcaagcagag ttgccatagc aacaccaatc aatgcatatt cgattgcagt
     1201 aacaccacgt tcatccttcg cgaaacgcgc aaagcactgt ttagttttac catagtactt
     1261 agtaataaaa gtattcatgt tttcctcgta tattattatt taaattcaaa ccaccagctt
     1321 catctgcgtg tcaccggtat cccatatttt ttggatgtgg ctataattat ccattcccgc
     1381 tatttagtct tcaaaaaaaa ttacatttaa tatcatttat cttgattaag aaatagtaag
     1441 actaaaaaaa caagcgtcgt cagccagcac actgctaaca cggtaagaac gctaataaaa
     1501 acactccact gcttcgttct ataccattca atatttttgt cagtatgttt tattgggagc
     1561 agctcttcag tagagaattc ctggctgtta tcattctcag gaatgtttgc atcagtgttt
     1621 acttccctgg cctcgctatc gttggtatag ctactactgt cttcatcact gtcacgctca
     1681 ttctcacaga catgtttctc gacctttccc agatatctga cgccttttcg cggaatggtt
     1741 ttaattagag aactcgacaa acctgcgacg tctaatgcct ttctaagcat gtaaatctgc
     1801 ccataataac tgctgtcgct taccgaacct ttctgctccc gccaaacctc attgataata
     1861 tgttgcttat cggattctcc ctgtagtaaa aggagtaaaa atcgatagcc gttttcagac
     1921 agattgatta cattgcctgt actggtggtg atacgtcgag acgctgggta aaacttaact
     1981 ccctgcccta aagaaaaata aaaacggtca tcgctcataa gtaccgaccc ctgttttctt
     2041 cttcacatga ctactcatta caactttgta tgaagtaaga tgcaaaatca tattgtttac
     2101 attaagttaa gcaattaggt aatgactcca acttactgat agtgttttat gttcagataa
     2161 tgcccgatga ccttgtcatg cagctccacc aattttgaga acgacagtga cttccgtccc
     2221 agccttgcca gatgttgtct cagattcaga ttatgtcgct caatgcgctg agtgtaacgc
     2281 ttgctgataa cgtgcagctt tcccttcagg cgtgattcat acagcggaca gccatctgtc
     2341 atccatacca cgacctcaaa ggccgacagc aggctcagaa gacgctccag tgtggccaga
     2401 gtgcgttcac cgaagacgtg cgccacaacc gtcctccgta tcctgtcata cgcgtaaaac
     2461 agccagcgct gacgtgattt agca
//
LOCUS       DAEEDS010000216         2433 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEEDS010000216 DAEEDS010000000
VERSION     DAEEDS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.222"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            65..1543
                     /locus_tag="KLH32_004201"
                     /pseudo
     CDS             65..1543
                     /locus_tag="KLH32_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2433
                     /locus_tag="KLH32_004202"
     CDS             1540..>2433
                     /locus_tag="KLH32_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBE0140788.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT          630 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa caa
//
LOCUS       DAEEDS010000217         2392 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEEDS010000217 DAEEDS010000000
VERSION     DAEEDS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.223"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..109
                     /locus_tag="KLH32_004203"
     CDS             <1..109
                     /locus_tag="KLH32_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBE0140789.1"
                     /translation="LQTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            102..1061
                     /gene="ssuA"
                     /locus_tag="KLH32_004204"
     CDS             102..1061
                     /gene="ssuA"
                     /locus_tag="KLH32_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBE0140790.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1058..2203
                     /gene="ssuD"
                     /locus_tag="KLH32_004205"
     CDS             1058..2203
                     /gene="ssuD"
                     /locus_tag="KLH32_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBE0140791.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2212..>2392
                     /locus_tag="KLH32_004206"
     CDS             2212..>2392
                     /locus_tag="KLH32_004206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBE0140792.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          654 c          673 g          538 t
ORIGIN      
        1 tctgcaaacc catcttgata ccgcgctaga aactttctgg caggcattgc accgccgcga
       61 tgttcaggtt cctgaccttc tgtctctgcg aggtaatgcc catgcgtaac atcattaaac
      121 tggcgctggc gggattgctt agcgtctcta cttttgcggt tgctgcagaa tcctcgcctg
      181 aagcgttacg tataggctat cagaaaggca gtattggtat ggtgctggca aaaagccacc
      241 agttactgga aaaacgctat ccgcaatcaa aaatctcctg ggtggagttc cccgcgggtc
      301 cgcaaatgtt ggaagcgtta aacgttggca gtattgatct cggcagtacc ggggatattc
      361 cgccaatctt tgcccaggct gccggggctg atttggtata tgtgggcgtc gagccaccga
      421 agcccaaagc cgaagtgatt ctggtggcag aaaacagccc gatcaaaacc gtagccgatc
      481 ttaaaggtca caaagttgcc tttcagaaag gttccagttc acacaacctt ttactgcgtg
      541 cactgcgtca ggccggactt aaattcaccg atatccagcc cacttacctg acgcctgccg
      601 atgcccgcgc cgcgttccag caaggtaacg ttgacgcctg ggctatctgg gatccctact
      661 actccgctgc gttattacag ggcggcgtgc gggtgctgaa agacggcacc gatctcaatc
      721 aaactggatc gttttatctg gcagctcgtc cctatgcaga aaaaaacggc gcttttattc
      781 agggcgtact ggcaaccttt agtgaggccg atgcgttaac ccgcagccag cgcgaacaaa
      841 gcatcgcttt actggcaaaa acgatgggct taccggcacc ggtgattgcc tcttacttag
      901 atcatcgccc tcctaccacc atcaaaccgg ttaacgccga ggttgccgcc ttacagcagc
      961 aaacggcaga tctgttttat gaaaaccgtc tggtgccgaa aaaagtcgat attcgccagc
     1021 gcatctggca gcccactcaa ctggaaggaa aacaattatg agtctgaata tgttctggtt
     1081 tttaccgacc cacggtgacg ggcactatct gggaacggaa gaaggttcac gcccggttga
     1141 tcacggttat ctgcaacaaa ttgcgcaagc ggcggatcgt ctgggctata ccggtgtgct
     1201 gatcccaacg gggcgatcgt gtgaagatgc gtggctggtt gcagcgtcga tgatcccggt
     1261 gacgcagcgg ctgaagtttc ttgtcgccct gcgtcccagc gtaacctcac ctactgttgc
     1321 cgcccgccag gccgccacgc ttgaccgtct ctcaaatggt cgtgcgttgt ttaatctggt
     1381 cacaggcagc gatccacaag agctggcagg cgacggcgtg ttccttgatc atagcgagcg
     1441 ctacgaagcc tcggcggaat ttacccaggt ctggcggcgc ttattgctgg gggaaaccgt
     1501 caatttcaac ggtaaacata ttcatgtgcg cggagcaaaa ctgctcttcc cgccgattca
     1561 acagccttat cctccgcttt actttggcgg atcgtcagat gtcgcccagg agctggcggc
     1621 agaacaagtt gatctctacc tcacctgggg cgaaccgccg gaacttgtta aagagaaaat
     1681 cgaacaagtg cgggcgaaag ctgccgcgta tgggcgcaaa attcgtttcg gtattcgtct
     1741 gcatgtgatt gttcgtgaaa ctaacgacga agcgtggcag gccgccgagc ggttaatctc
     1801 gcatcttgat gatgagacta tcgccaaagc acaagccgca ttcgcccgga cggattccgt
     1861 aggacaacag cgaatggcgg cgctacataa cggcaagcgc gacaatctgg agatcagccc
     1921 caatttatgg gcgggcgttg gcttagtgcg cggcggtgcc ggaacggcgc tggtgggcga
     1981 tggtcctacg gtcgccgcgc gaatcaacga atacgccgcg cttggcatcg acagctttgt
     2041 gctttcgggc tacccgcatc tggaagaagc gtatcgggtt ggcgagttgc tgttcccaca
     2101 tctggatgtc gccattccgg aaattcccca gccgcagccg ctgaatccgc aaggcgaagc
     2161 ggtggcaaat gattttatcc cccgtaaagt cgcgcaaagc taaggaacac gatgatggca
     2221 acgccagtga agaagtggtt attgcgcgtt gccccctggt ttttaccggt gggcatcgtg
     2281 gcggtgtggc aactggcctc ctcggttggc tggctgtcga cgcgtatttt gccttcaccg
     2341 gaaggagtgg tgacggcgtt ctggacgctc tccgccagcg gcgaactgtg gc
//
LOCUS       DAEEDS010000218         2372 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEEDS010000218 DAEEDS010000000
VERSION     DAEEDS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.224"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..823)
                     /locus_tag="KLH32_004207"
     CDS             complement(<1..823)
                     /locus_tag="KLH32_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0140793.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1423..1671)
                     /locus_tag="KLH32_004208"
     CDS             complement(1423..1671)
                     /locus_tag="KLH32_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE0140794.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2026..>2372)
                     /locus_tag="KLH32_004209"
     CDS             complement(2026..>2372)
                     /locus_tag="KLH32_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0140795.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          610 a          437 c          576 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggtaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttaggtaa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg caatttttcc ttaaggtatg aaaaaaggag
     1081 ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta tataaaaaac
     1141 tgtgttaagg aggctgtcca ttagcggttt tttattcttc tggaaccgcg gcatctttgc
     1201 gtatcttttt gtcttctcac cgtctggtcg gtgttttgct gagactgcta atttcctgtc
     1261 tttgctagtg ctgtactggt actgcccaat catgattggc gggggcgggc ggagttgaga
     1321 ccgcagacac gtcgtatgca agaacgtgct gcggctggct ggtgaacttc cgatagtgcg
     1381 agtattgaat gatttccagc cgttactgat tttacgtgct aattagtgaa caaaccactc
     1441 gtcagcagac tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt
     1501 atcggcggtg cgcgtaacag aaaggccatc gttgctggtg gcttttacga tcacctctac
     1561 atcgtcataa cgtttactga tgcgtcgggt taattcttcc tttaacgcat ccacagcacc
     1621 ggttggcatt ttagtcattt tctctttggc tatgcagatt tcaaaacgca taaaaatccc
     1681 tctgcactgt gtttttatac agtattattt ttaactgtat ggataaacag tgtcaagagt
     1741 gagggaaagt gattttttgg ggcatgcatg gggcaaaaat atttgttgtg gggcattttg
     1801 gggcatgatt gggacacttt gttacatatg aactttgccg attttcatat gacatggcaa
     1861 tccaacgctt tgattattct aaagaaaaca catgctcttg ggcgttcttt agtgattttt
     1921 aaaaatcccg tatcacgcag ttaaagtggc gggcatactc ttcaaggctg gtgatcccca
     1981 accgcaccca tttaggatgt gaccattgcg gtagaccgat gtaaatcata acgcgtgaag
     2041 gatctcttcc acgctacgca cacgggcgat gcgcgggtag atatgattaa tgctgttatt
     2101 gtgttgctcg gcgctggcgg cactacaggc gtcttcggca atcaccagat taaagccaag
     2161 ttcccaggca ttgcgggcgg tggattcaac accgatattg gtcgagatcc cacataacac
     2221 tattgtatcg atacctcggc ggcgcagttg caactccaga tccgtaccgt aaaacgcacc
     2281 ccactgacgt ttgatgattt cgatatcact gtcagttgca cctaatgcag ctggatgttg
     2341 ccaccagttt tcgggcaaca ctttagcggg gg
//
LOCUS       DAEEDS010000219         2341 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEEDS010000219 DAEEDS010000000
VERSION     DAEEDS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.225"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(45..314)
                     /locus_tag="KLH32_004210"
     CDS             complement(45..314)
                     /locus_tag="KLH32_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0140796.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            371..1039
                     /locus_tag="KLH32_004211"
     CDS             371..1039
                     /locus_tag="KLH32_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0140797.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1355..>2341
                     /locus_tag="KLH32_004212"
     CDS             1355..>2341
                     /locus_tag="KLH32_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0140798.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSF"
BASE COUNT          690 a          540 c          443 g          668 t
ORIGIN      
        1 cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt
       61 tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga
      121 tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc cagatctaca
      181 gcatcctgaa gtggcgcaat atgctcactg aatccctgga tgtagaactg tgtggtgacg
      241 gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc aatatcgcta
      301 tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa ttcacaaata
      361 aaactataat atggtgaaat taatgaaaaa aaacacagat gatggggcta aaatttacac
      421 accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc ggcttgcatg
      481 gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag gtaacaacca
      541 tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga gtagtctgat
      601 atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg ctggggaatc
      661 aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcctgcgg cgttacatgg
      721 tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa atatatctac
      781 aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa ctctatacgg
      841 agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat
      901 ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct taacacatat
      961 actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag taatgttttc
     1021 cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac gtgctacggt
     1081 ttcctttacg atttggggtt gcgactttac ctctattgat aatgcattcc ggcagaacgt
     1141 tcaaatatga acgtacgata tttaactaac cgaaaaacaa aatatagcac aggcgagaca
     1201 tttaccatct aagaaaatag tatcgttttt ttacagttaa atcaacatca cttccttaaa
     1261 atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gcttattatt ccccagtatc
     1321 acattttatc aggatatcct tctgcgaggt tattatgctt cctgtaaata atccccccct
     1381 atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc acaataatta
     1441 tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc
     1501 ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact tatcttgttt
     1561 aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg taagcaataa
     1621 ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg caagcagtaa
     1681 tcaattatct gcacttcctg aattaccagt gtcgctggaa tatataaatg tgagtgataa
     1741 ccatttgttc gcacttcctg aattacctgc gtcactagaa tatattaatg taagtgacaa
     1801 tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg cagccagaaa
     1861 tgctttggaa gtaataccag attttccaga aagagatgat catattataa gaatattctg
     1921 gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca gttctgatag
     1981 cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt tgttacaaca
     2041 aaccgcccaa ccggactacc acggcccacg gatttacttc tccatgagtg acggacaaca
     2101 gaatacactc catcgccccc tagctgatgc cgtgacagca tggttcccgg aaaacaaaca
     2161 atctgatgta tcacagatat ggcatgcttt tgaacatgaa gagcacgcca acaccttttc
     2221 cgcgttcctt gaccgccttt ccgataccgt ctctgcacgc aatacctccg gattccgtga
     2281 acaggtcgct gcatggctgg aaaaactcag tgcctctgcg gagcttcgac agcagtcttt
     2341 c
//
LOCUS       DAEEDS010000220         2190 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEEDS010000220 DAEEDS010000000
VERSION     DAEEDS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.226"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..701
                     /locus_tag="KLH32_004213"
     CDS             <1..701
                     /locus_tag="KLH32_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140799.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNG
                     NTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAF
                     NGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIP
                     VLDVSDISVKLQEA"
     gene            703..1308
                     /locus_tag="KLH32_004214"
     CDS             703..1308
                     /locus_tag="KLH32_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBE0140800.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1295..>2190
                     /locus_tag="KLH32_004215"
     CDS             1295..>2190
                     /locus_tag="KLH32_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0140801.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          533 a          508 c          611 g          538 t
ORIGIN      
        1 acgcccgtgc cggtgtgccg ggcagcgatg aggaaacccg tgcggacttt gaaaaccgcc
       61 gacgtaattc agttgcccgt aatgcccgga atattctgga agccatccgg ggtgaaatac
      121 tctccacggt agaaaacgtg gtggatgttt acgtcaccca taatccgaaa aaaacagaac
      181 aaaaagccgg agtcagtcag tatccattaa cacccggttc gctttatgtt ggcgtgtacg
      241 gcggcagccc ggcagatatc gcggcggcca tctggcgtaa ggctccgccg ggtattgata
      301 tgaatggcaa cacaacgttc actgttgcag ataaggagta cgatccgccg tatcctgaat
      361 acgtgatcag ctggcagaca ctcaaacccg tcagtctgca tgtcagtgtg acgctgaaaa
      421 aaagtgacta cctgccttca gatattaccc gacaggtaca gcaatccgtg ctggacgcat
      481 ttaatggtac agatggtggt ctgcgggcaa gagttgcctc tgttgtctct gccgggcgct
      541 actatgccgg catttacaaa accgatccgg aacatattga tattctgggc cttactgtga
      601 gccgtgatgg ttcgtcatgg acaacggctg tcactttcgg gatagatgag attccggttc
      661 tggatgtgtc ggatatcagt gtgaaactcc aggaggcata acgtgcagaa tgtggctgcc
      721 actgtgcttg cgcagtatgc tgccagcccc cgactcaatg ccctcattaa cagctttaac
      781 gcagcgcttt cccccgacag ttttatcagt gatttttatg gtcttatctg gaacatcgat
      841 accgcagaaa agtatggtct tgatgtctgg ggaaagattg tgggtgtcag ttgctggctg
      901 acggtaaagg acgattttaa ttacctgggc ttcagcgagt ccaggatgga caccccggta
      961 atggatgatc cctgtccgtt taatcaggca ccgttttaca acggaaaatc ggatacccgg
     1021 actgttgacc tgtctgatgc tgtataccgg cggctgatac tgatgaaagc catgtcgaac
     1081 attactgact gttccgttcc ggatattaac ctgatgctga gatttatgtt cggaaaaaaa
     1141 cgccgggctt atgttctgaa taatggcggg ctgaggatga gttacgtctt tgagtccgcg
     1201 ctctcgttgg cagaactggc gattatccag tcgtcgggtg cactgccatc cccgccgggt
     1261 gtttatgttt cagtagtttt aaaggagtcc cgtaatgaag gccagtgata aaccccgcca
     1321 gctggcggtc ccctttgcga gtaccggaga taaaaaccgt atcccggaca aggcgacaca
     1381 gcagaccaga gagagcggta atgctgcgta tgattcaggt tttcctccgg tgaccatgac
     1441 agcggtctca gcgggaggta taccgccaca cggcaaggat tttaacggtc tgatgtacga
     1501 tattaccgca gcaatacggt tcgcccaggc tggcggtttg tacacgtata atgccggttt
     1561 tgcgggggcc attggtggat atgcaaaagg agccattctc gccggagtcg caacaacagc
     1621 ggtctggctg aataccacgg acgataacct gaccgatcct gaaggctccg acagtgcggg
     1681 ctgggtaaat cttcttgagg atccgaaaag gatattcctg cggcagaaga acaatctgtc
     1741 agaccttcag aataaaggga cggcacggga taatcttcag gtttacagta aagagcagtc
     1801 agatcaacgc tatgttcatc gggaaggcga taaaataacc ggagagctga aaatccgtgg
     1861 tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg atttttcgtc gttcggaaga
     1921 gtgcctgcac cttattccca ccagtgaagg tcagggggaa aatggcgata ttggtcccct
     1981 gcgcccgttc accattaatt tgcggacggg tgaaatatcc atgtcgcata aagtgtctgt
     2041 tggcggtggt tcgcaggtca atggtgcgct gggtatcggc gttcagaacg ccctgggggg
     2101 gaattcaatt gttcttggtg ataatgacac cggatttaaa cagaatggag acggtattct
     2161 ggatgtttat gctaatagtc agcgtgtatt
//
LOCUS       DAEEDS010000221         2137 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEEDS010000221 DAEEDS010000000
VERSION     DAEEDS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.227"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..491
                     /locus_tag="KLH32_004216"
     CDS             <1..491
                     /locus_tag="KLH32_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140802.1"
                     /translation="ADFTYVSTWQGFVYVAFIIDVFAGCIVGWRVSSSMETTFVLDAL
                     EQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGL
                     YKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDL
                     AA"
     gene            complement(869..1015)
                     /locus_tag="KLH32_004217"
                     /pseudo
     CDS             complement(869..1015)
                     /locus_tag="KLH32_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1069..1251
                     /locus_tag="KLH32_004218"
     CDS             1069..1251
                     /locus_tag="KLH32_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBE0140803.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(1352..1681)
                     /locus_tag="KLH32_004219"
     CDS             complement(1352..1681)
                     /locus_tag="KLH32_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBE0140804.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1853..>2137)
                     /locus_tag="KLH32_004220"
     CDS             complement(1853..>2137)
                     /locus_tag="KLH32_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140805.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          575 a          494 c          531 g          537 t
ORIGIN      
        1 tggctgattt tacttacgtc agcacatggc agggcttcgt ctatgtggcg ttcatcattg
       61 atgtgtttgc cggatgcatc gtggggtggc gggtctcatc gtccatggaa acgacattcg
      121 tgctggatgc actggagcag gcgttatggg cccgtcgacc gtccggcacg gtccatcaca
      181 gtgataaagg ttctcagtat gtatcgctgg cctacacaca gcggcttaag gaagccggat
      241 tactggcatc aacaggaagt acaggcgact cgtatgacaa cgcgatggcg gagagcatca
      301 atggtcttta caaagcggag gtaatacacc gtaagagctg gaaaaaccgt gcagaagtgg
      361 aactggccac actaacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg
      421 gccacatccc tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc
      481 tggcagcctg agttcacaga taaaacactc tccaggaaac ccggggcggt tcaaatagca
      541 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      601 aaggcgacgt agcccggtac aagagtaacc cacgttagcg cttggcgacg gcggaacaaa
      661 gctgattcgc gacgtaagaa agtggcaggc tcatatgacg gacctctgta atgcggtatc
      721 caacccgcga atatcagcat gattcaccgt cggatttact gctacgtcgc cctcagggaa
      781 ttaaacctaa aaagaaaatc gtcaaaatta gagattccgg tttgacacgg aaagtcatat
      841 cagcgtcaac ggagcaccgt attgacgctt atttattggt gagaactacg ttccatggca
      901 ggagttcgtc aacacggttg gagggccatt caggcagtat gctcagaata tggcgcagat
      961 acgcttccgg atcgataccg ttcagacggc aggtgcctgc ttttccggca acaagcagga
     1021 atctcagcgc tgaagcagga attagaggta aaaacgccct accgggccat gaatcacccg
     1081 gtcattggag tagtaaccaa agcagattta gccagcatgg aacaaatatc tttggtgaaa
     1141 agttggttac gggaagctgg agcgcacaac gtgttagtaa ccagtgcagt taataacaat
     1201 ggagttacag aactttttgc cttgctgcat acagaagaag gctgttgtta atttcgattg
     1261 gttctcagat gagaatgatg acggtgatac acagataaga atcaatgagc aatgagggtt
     1321 gccgggcaac cctcattgaa taaaacggga attacttcgc ttcgccgttt ttcatttcgc
     1381 ccatagcttt cagctttttg gagatatcgc ggcgttcttt ggagagctcg gcatttttga
     1441 tgatgtaatc gtcaacgcga tcttcatagt cacctttcat gctggcgatg atgccctgga
     1501 ttgcttcaac gctcatccct ggcttgatgt aatcgctcag gttgtccagc aacaggacgc
     1561 gcttctggtt gtcacggatc tttttctcaa cgtcctgaat ttcacgttgc agtttgttct
     1621 tacgacggaa cagacgaaca aattccagta cgtcctggaa tgaaggcttg gtagtttcca
     1681 tttttatacc cctgataatg tgagagtcgg attcgtttaa gcaaccgcta ttcgttaggg
     1741 ccaacattac tcatagctgc cgcagatgac aatgctttta tcccttttca ctatcatacc
     1801 ctttatcctt gctgaatcga agcagcagca agatgattct gaagttcaga aattatttgc
     1861 gcaaggcccg gcaaatcaga ttcgatagca actcaagctg atgtgccgtt tccatgttca
     1921 gccacacata accatagatt ggtgtttcca cgactttcag gtcatggtgg ttatcgagca
     1981 actgacgcag ctcttccaca gcatcgttca atttttccgt attggcaaaa accggctgcg
     2041 gattaccttc gtacagcgca tgaacaaggc tcagcagtat ttgctgcatc atgtgctggg
     2101 tatcacaaag tttttgcgcg ttcaataaca cgaaatg
//
LOCUS       DAEEDS010000222         2126 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEEDS010000222 DAEEDS010000000
VERSION     DAEEDS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.228"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            43..222
                     /locus_tag="KLH32_004221"
     CDS             43..222
                     /locus_tag="KLH32_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140806.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            274..468
                     /locus_tag="KLH32_004222"
     CDS             274..468
                     /locus_tag="KLH32_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140807.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1267..1603)
                     /locus_tag="KLH32_004223"
                     /pseudo
     CDS             complement(1267..1603)
                     /locus_tag="KLH32_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          436 g          571 t
ORIGIN      
        1 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
       61 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
      121 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
      181 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
      241 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
      301 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
      361 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
      421 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
      481 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatattca
      541 ctgacgagct tgccggggca cctttgtttg ccggtcattc tggtatcatg cagatattta
      601 cggcgtacaa gcccactagc atgacattgt aataaggcct gagcctaacg ctttaatgtg
      661 tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt aagcgtccag
      721 cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc aaataagtga
      781 tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat gggagcggat
      841 gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc cgatctgttt
      901 aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca tcgttaatac
      961 gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct gcatatggat
     1021 cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat ggagtagcac
     1081 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac ttcttcagca
     1141 cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa ctaatacatc
     1201 agaatatgca ttatgttaaa taaatatgaa aagatggttt aaataccccg ttacttttga
     1261 cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt tatttctatt
     1321 agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt ggttatgctt
     1381 taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg tgttaatgtc
     1441 agttcccctt tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc
     1501 cctggattat agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg
     1561 cagatccagt ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa
     1621 ttgagtcgaa aataagcact actccataca ggataattag aggtcagttt gattattcac
     1681 aattcatcat cagcattttc tatttctgac gaaatcaaca tgaaaataac catatatgat
     1741 aattattata ataacggctt taattggaat acatatatta caacgtatta tatataattg
     1801 gtattctggg aactatattc tcaaaatata gtagaaacga ggtatgtttc tggtggaaag
     1861 gacagtggga ttaaaaagta agaattgata aaaaaaacgc cagcaacaca tagtgccggc
     1921 ggagggaata ccccatggag aaaatgtgat gcctagagca tcatgaatat ataattaaaa
     1981 atagttagcg ttgtcaatac tttcacaaaa ataattttcg tagtatagaa agatattttt
     2041 atgcatgacc tacctgaatt tgctccgggt agaggttata aataaaaatt gaatcacgac
     2101 aaacacaata ttcatagtat ggcgat
//
LOCUS       DAEEDS010000223         2111 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEEDS010000223 DAEEDS010000000
VERSION     DAEEDS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.229"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KLH32_004224"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KLH32_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE0140808.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="KLH32_004225"
     CDS             complement(983..1813)
                     /locus_tag="KLH32_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBE0140809.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          516 a          481 c          470 g          644 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca g
//
LOCUS       DAEEDS010000224         2096 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEEDS010000224 DAEEDS010000000
VERSION     DAEEDS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.230"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="KLH32_004226"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="KLH32_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBE0140810.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="KLH32_004227"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="KLH32_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBE0140811.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAEEDS010000225         2062 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEEDS010000225 DAEEDS010000000
VERSION     DAEEDS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.231"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1948
                     /locus_tag="KLH32_004228"
     rRNA            <1..1948
                     /locus_tag="KLH32_004228"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          530 a          453 c          651 g          428 t
ORIGIN      
        1 ctaacgtccg tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc caaagtcatg
       61 gttaagtggg aaacgatgtg ggaaggccca gacagccagg atgttggctt agaagcagcc
      121 atcatttaaa gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga agatgtaacg
      181 gggctaaacc atgcaccgaa gctgcggcag cgacgcttat gcgttgttgg gtaggggagc
      241 gttctgtaag cctgtgaagg tgtgctgtga ggtatgctgg aggtatcaga agtgcgaatg
      301 ctgacataag taacgataaa gcgggtgaaa agcccgctcg ccggaagacc aagggttcct
      361 gtccaacgtt aatcggggca gggtgagtcg acccctaagg cgaggccgaa aggcgtagtc
      421 gatgggaaac aggttaatat tcctgtactt ggtgttactg cgaagggggg acggagaagg
      481 ctatgttggc cgggcgacgg ttgtcccggt ttaagcgtgt aggctggttt tccaggcaaa
      541 tccggaaaat caaggccgag gcgtgatgac gaggcactac ggtgctgaag caacaaatgc
      601 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
      661 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      721 aaatggtgcc gtaacttcgg gagaaggcac gctgatacgt aggtgaagcg acttgctcgt
      781 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      841 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt
      901 gatggggtta gcgcaagcga agctcttgat cgaagccccg gtaaacggcg gccgtaacta
      961 taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacctgca cgaatggcgt
     1021 aatgatggcc aggctgtctc cacccgagac tcagtgaaat tgaactcgct gtgaagatgc
     1081 agtgtacccg cggcaagacg gaaagacccc gtgaaccttt actatagctt gacactgaac
     1141 attgagcctt gatgtgtagg ataggtggga ggctttgaag tgtggacgcc agtctgcatg
     1201 gagccgacct tgaaatacca ccctttaatg tttgatgttc taacgtggac ccgtgatccg
     1261 ggttgcggac agtgtctggt gggtagtttg actggggcgg tctcctccta aagagtaacg
     1321 gaggagcacg aaggttggct aatcctggtc ggacatcagg aggttagtgc aatggcataa
     1381 gccagcttga ctgcgagcgt gacggcgcga gcaggtgcga aagcaggtca tagtgatccg
     1441 gtggttctga atggaagggc catcgctcaa cggataaaag gtactccggg gataacaggc
     1501 tgataccgcc caagagttca tatcgacggc ggtgtttggc acctcgatgt cggctcatca
     1561 catcctgggg ctgaagtagg tcccaagggt atggctgttc gccatttaaa gtggtacgcg
     1621 agctgggttt agaacgtcgt gagacagttc ggtccctatc tgccgtgggc gctggagaac
     1681 tgaggggggc tgctcctagt acgagaggac cggagtggac gcatcactgg tgttcgggtt
     1741 gtcatgccaa tggcactgcc cggtagctaa atgcggaaga gataagtgct gaaagcatct
     1801 aagcacgaaa cttgccccga gatgagttct ccctgaccct ttaagggtcc tgaaggaacg
     1861 ttgaagacga cgacgttgat aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg
     1921 gtactaatga accgtgaggc ttaaccttac aacgccgaag atgttttggc ggattgagag
     1981 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     2041 tagcctggcg gccgtagcgc gg
//
LOCUS       DAEEDS010000226         2034 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEEDS010000226 DAEEDS010000000
VERSION     DAEEDS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.232"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>2034
                     /gene="sat"
                     /locus_tag="KLH32_004229"
     CDS             <1..>2034
                     /gene="sat"
                     /locus_tag="KLH32_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBE0140812.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          746 a          359 c          401 g          528 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca gatc
//
LOCUS       DAEEDS010000227         2033 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEEDS010000227 DAEEDS010000000
VERSION     DAEEDS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.233"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..363)
                     /locus_tag="KLH32_004230"
     CDS             complement(<1..363)
                     /locus_tag="KLH32_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0140813.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="KLH32_004231"
     CDS             complement(430..1644)
                     /locus_tag="KLH32_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBE0140814.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DAEEDS010000228         2032 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEEDS010000228 DAEEDS010000000
VERSION     DAEEDS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.234"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..131
                     /locus_tag="KLH32_004232"
     CDS             <1..131
                     /locus_tag="KLH32_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE0140815.1"
                     /translation="YYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(831..905)
                     /locus_tag="KLH32_004233"
     tRNA            complement(831..905)
                     /locus_tag="KLH32_004233"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(870..872),aa:Gly,seq:tcc)
     gene            complement(909..984)
                     /locus_tag="KLH32_004234"
     tRNA            complement(909..984)
                     /locus_tag="KLH32_004234"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Thr,seq:tgt)
     gene            complement(987..1063)
                     /locus_tag="KLH32_004235"
     tRNA            complement(987..1063)
                     /locus_tag="KLH32_004235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Arg,seq:tct)
     gene            complement(1065..1140)
                     /locus_tag="KLH32_004236"
     tRNA            complement(1065..1140)
                     /locus_tag="KLH32_004236"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1105..1107),aa:Ile,seq:cat)
     gene            complement(1317..2006)
                     /locus_tag="KLH32_004237"
     CDS             complement(1317..2006)
                     /locus_tag="KLH32_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBE0140816.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          542 a          533 c          432 g          525 t
ORIGIN      
        1 cttactacac acgcaaaatg agtcagggaa aacgacacaa tcaggcgctt atcgccctgg
       61 cgagacgacg ctgcgacgtt ctgttcgcca tgatgcgcga cgggactttt tataccccgc
      121 aggggtcata acatgcttga caacttaata ggggcccccc ccgacagacc tgtgctatcg
      181 gaaagaaaaa aggccatcag caaaactctg atgacctgaa tcacctttac caatattgta
      241 tgaaaaaaca cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta
      301 gcattcttaa ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat
      361 ccccaatgtt tttgctggcg ggtccttttt tctacggtcc tcctccccgg aggggggcat
      421 ttcattattt ttctaatcgt tactggtgag aaggcggaag aaaaagccag ttcttcgccc
      481 ctaaataaca gcatgattcc agtccatgca aaatctgatt tccagctcac acataccgag
      541 cgctgtacaa aaaaccgcca ggcaaggcag ttaagagtgt gtccccgggg tttgcttaga
      601 atattttgta agttgtccga agagatattt acaacaccag aatgatgaat catcagtccc
      661 ctgccagagg ttcactacgt aacctgaccg acaatgcacc attctggtgc tgaaaaaaga
      721 gcactggaac tgcaacaaaa caacatcaca gaacaggaga tggtaaggag tcagacattc
      781 ccacacaata ttgtgtcagt gcaaataaca accttcgtct caaatctcgc tggagcgggc
      841 agcgggaatc gaacccgcat catcagcttg gaaggctgag gtaatagcca ttatacgatg
      901 cccgcatatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      961 ctctgcctgc tgagctaagt cggcgctggc ccaccaccga ggactcgaac ctcggaccgt
     1021 caacttagaa ggttgatgct ctatccggat gagctagtgg tggttggtgg cccttgctgg
     1081 acttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1141 ggaggcagaa taataaccat atgtcatcac atctgcaaac tcatctgacc accagcacgt
     1201 ttaacgtcct gtgccatttt tcaggcataa aaaaaccgct cggcggcggg ttttgcttac
     1261 tttaccatcg cgtacaaaat cggcaaagta tcagatttgc atgagatata tgccttttaa
     1321 tctacttttg caatactttg ctttgaaaat gccgcttttt gttctgaacg tgttctcatc
     1381 acaaacaata aagcctcact atccagtcgg tgaaaaatgt gtttcattac aacccagtga
     1441 ccagtaaatg ttttggacca gtttttggtt gttactcccg ccagtaatgc cagctcctga
     1501 tattcataac cttccccacc aaaaagttct gcttttaccg cctgcgccgc cagccagatt
     1561 aattttttca ggcgttcctg tgttttctct gcaattttcc tagtgccaga atgagcctta
     1621 aactcttccc acgcccagtg ggttatggtg atctgatact cccagcaaat acttccgctg
     1681 taacaccaca acaaccaggc tttatagtgc tcttcaagag acagaacagc ccgccgccac
     1741 gatgatgtcg aaaacgcaac ctgactgacc agaggaattg acgccccctt cgccagtgat
     1801 tgttttcccg ggattggtgg attatcccgc gttatcattt ttccggtcac ctcatcgcgg
     1861 taccggattt ttttacgcct gtaacgccct gtatcgaaca tggcattctc ctgccaggct
     1921 tcaagctgac cttttgtcgc cccactcaaa tcagcggtgg cgataatgag ctgctcacga
     1981 acaaactgta aatactggtt attcatgcac actccagctc tgtgattttt at
//
LOCUS       DAEEDS010000229         1998 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEEDS010000229 DAEEDS010000000
VERSION     DAEEDS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.235"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..371
                     /locus_tag="KLH32_004238"
     CDS             <1..371
                     /locus_tag="KLH32_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140817.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGTWYNVASI"
     gene            374..918
                     /locus_tag="KLH32_004239"
                     /pseudo
     CDS             374..918
                     /locus_tag="KLH32_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            942..1998
                     /locus_tag="KLH32_004240"
                     /pseudo
     CDS             942..1998
                     /locus_tag="KLH32_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
BASE COUNT          547 a          461 c          534 g          456 t
ORIGIN      
        1 atccatccag gcctgttgtt gcagagcttg gcgatgattc aggctggcat tttttcagcc
       61 agagaaaccc ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt
      121 acggaaactt cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg
      181 gccatgaaat gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt
      241 cgggacacgg gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg
      301 ttgacggtgt gtattaccga ccactgcaaa aactgattaa tggcacctgg tataacgtag
      361 cgagtattta acaatgttgc atttaaaaaa tattactacg ggtaatccga aaacagcaga
      421 acaatatcag atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa
      481 ctggtatgaa gagctgaaga atttcgccag gacacaatta aaataactta caccggagac
      541 ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
      601 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
      661 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
      721 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgttgct ggggctaatc
      781 agcgacgaag accgggaaaa actgaaagcg taccgcattt acgcgaaatc gctgcaggcg
      841 atggatttca gtgtcatcac tgataaaacc tcatacaacg ccattgaatg gcccgcttct
      901 ccggaaattt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
      961 gtgcgctgac tgatggcgca ggtatctcca tgtccggata ccatattatt ctgaaatccc
     1021 gggtaaacac cccggaagtg gtgatgcaca ctgttgctga tgtgatgaca ggaaacgatg
     1081 gtgaatactg tttccatgcg cggactggaa aatatggtgt gtatctgaaa caggactggc
     1141 acaacgagta caacgttggc gacattgctg tatatgagga ctcaaagccc ggtacgctga
     1201 atgactttct gattgctcct gatgagggcg acctgaaacc agatgtcgtc aaacgctttg
     1261 aggaaatggt ggcgcaggcg cagcagagca ggccaaaaat gacattgatg ctgcgctgac
     1321 cggcactctg aaaacggcta accatctgtc tgaaatcgca gcagcaggcg aaaaggcaca
     1381 acagaagtcc cgggataatc tggggctgaa aagtgcggcc acgatggaag cacagagcga
     1441 catttacgac cggacaaaag gccgtctggc gatacccggc gcattcggct ttgggtgtgc
     1501 ttttctgcct gaagatgtta tccgttttga cactaagagt gatttcctgg cctgggtaag
     1561 gaatgcgctg ccaggtgaat attccgttgc tggcccctac ggcatcatca tacccgacac
     1621 acggtttgaa ggggtgctca gcatccggtg gactgatgca cgccctgaga caacagaacc
     1681 gcggtacaga gccaaatccc ttacttttta cggcattaac ggccccattt atcacacccg
     1741 ctaccgctac tggcccatat ccagactgac tgactgggtg aaaataaata taaccacaga
     1801 agatattatt tacagaatcg tggcgagctc tgtccgcaac agatggggag accctgacat
     1861 tggcgggctg attattgctg cgtaccaggg agaagctgac ggtgataaag tcatcagact
     1921 tgtcaggggg cagtcataca gaggctcacg actgggaccg gtggggattt cagtgcccag
     1981 tactcccacc ggaacgta
//
LOCUS       DAEEDS010000230         1969 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEEDS010000230 DAEEDS010000000
VERSION     DAEEDS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.236"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1037)
                     /gene="ipaH3"
                     /locus_tag="KLH32_004241"
     CDS             complement(<1..1037)
                     /gene="ipaH3"
                     /locus_tag="KLH32_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBE0140818.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGPRIYFSMSDGQQNTL
                     HRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQ
                     VAAWLEKLSTSA"
     gene            1779..>1969
                     /locus_tag="KLH32_004242"
     CDS             1779..>1969
                     /locus_tag="KLH32_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBE0140819.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLR"
BASE COUNT          544 a          345 c          489 g          591 t
ORIGIN      
        1 gcagaggtac tgagtttttc cagccatgca gcgacctgtt cacggaatcc ggaggtattg
       61 cgtgcagaga cggtatcgga aaggcggtca aggaacgcgg aaaaggtgtt ggcgtgctct
      121 tcatgttcaa aagcatgcca tatctgtgat acatcagatt gtttgttttc cgggaaccat
      181 gctgtcacgg catcagccag ggggcgatgg agtgtattct gttgtccgtc actcatggag
      241 aagtaaatcc gtgggccgtg gtagtccggt tgggcggttt gttgcgacag agactccctg
      301 atccgtgagg acagaggatt gtcttcgagg atgatagtgc aggtcggatc aaggctaagt
      361 atattttccg gaatgtgtgt gatgcgattc tcgcggcacc ggaaaaatat ctcggtttcc
      421 tctgagtgat gatttcttac aggtacggct ggtaggcttt ccagaagatt agtacttaca
      481 tcgagcgctt ccagtgattc aggtaactca gggagaaatg tcagctggtt atttcttact
      541 gagagcactt ccagcgatgt aggtaattca ggaagcatgg ttagctgatt gttatctgca
      601 ttaatatatt ccagcaatgc aggcaattca ggaagcatgg ttagttggtt gttatctaca
      661 tcaagatgtt tcagagatgc gggtaattca ggaagtgttg acaggcgatt gtcacaggcg
      721 tcaaggtatt ccagcgatgc tggcaattct ggtaatgata ttagggcatt ctgagtaatt
      781 tccagaacag tgatttgagg tggtaagttg tcaggtagcg aggacagatt taaacgattc
      841 agttgaagct cactgaactg attgatgaga cattctttaa gtagggagac cgcttcattc
      901 cgattttcac cggggagcgc ttgtttttcc catttatccc atgctgaaaa gtaatcagca
      961 tatgtaccgg aaatagtgtt ataaaaagaa ttttgggaca atgaaaagtt attatttatc
     1021 ggtaacatga tggacattca gttaaagccg atggacaaat ggctttgtta aaagaaataa
     1081 gtaggagttt ttatgaagtt cattccatga aatgaacttc agcacttttt tattataaaa
     1141 ggacaaacaa aattggctgt aaaaaaacga tactgtttca gttaactgtg aatagcttgc
     1201 ttgcaccgta ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacgtt
     1261 ctgccggaat gcattatcaa tagaggtaaa gtcgcaaccc caaatcgtaa aggaaaccgt
     1321 agcacgtcgt atgcaagaac gtgccacggc tggctgatgg atgttcgata gcgcgagttt
     1381 gaatgaaaat cagccggaga tgattttaca taattgctac ggaattattc aatacaggaa
     1441 ttgcttgcgt ctgcatggat tgacctgaaa tattcccgaa aatttctcta aaaaactcga
     1501 aaaaaatggt aactggttga atgtattaat atgcaatggt acgtgtcagg gattaaaaga
     1561 tgaacgtaaa tttattcaac gcattaattt taaagggttt tattgtttgt tgacgaaaac
     1621 aggaatcatg ttcggtctct ttttatctgt taaaagccag aagcatttcc ttcgctgact
     1681 ttatagtcaa ccataacaca cactctactg tctgagtcca gcgtttttta acattcttgt
     1741 taagattatg tgatctttag cgcgggagga aaatattgat gaaacagcct gcgcccgttt
     1801 atcagagaat tgcgggtcat caatggcgac atatctggct ttctggcgat atacacggtt
     1861 gtcttgagca gttgcgccgc aaattatggc attgtcgttt tgatccgtgg cgagatttac
     1921 ttatctcagt gggagacgtt atcgatcgtg ggccgcaaag tttacgttg
//
LOCUS       DAEEDS010000231         1919 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEEDS010000231 DAEEDS010000000
VERSION     DAEEDS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.238"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(226..429)
                     /locus_tag="KLH32_004243"
     CDS             complement(226..429)
                     /locus_tag="KLH32_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140820.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="KLH32_004244"
     CDS             complement(431..586)
                     /locus_tag="KLH32_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBE0140821.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="KLH32_004245"
     CDS             complement(789..1196)
                     /locus_tag="KLH32_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0140822.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="KLH32_004246"
     CDS             1273..1500
                     /locus_tag="KLH32_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0140823.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="KLH32_004247"
     CDS             1484..1906
                     /locus_tag="KLH32_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140824.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
BASE COUNT          468 a          414 c          492 g          545 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcg
//
LOCUS       DAEEDS010000232         1913 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEEDS010000232 DAEEDS010000000
VERSION     DAEEDS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.239"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(53..1702)
                     /locus_tag="KLH32_004248"
     CDS             complement(53..1702)
                     /locus_tag="KLH32_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBE0140825.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAEEDS010000233         1850 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEEDS010000233 DAEEDS010000000
VERSION     DAEEDS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.240"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..258)
                     /locus_tag="KLH32_004249"
     CDS             complement(<1..258)
                     /locus_tag="KLH32_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140826.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="KLH32_004250"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="KLH32_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBE0140827.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="KLH32_004251"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="KLH32_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBE0140828.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="KLH32_004252"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="KLH32_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0140829.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1469
                     /locus_tag="KLH32_004253"
     CDS             1254..1469
                     /locus_tag="KLH32_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140830.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            1572..1850
                     /locus_tag="KLH32_004254"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="KLH32_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          409 c          467 g          467 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ttatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatctgaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa
//
LOCUS       DAEEDS010000234         1833 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEEDS010000234 DAEEDS010000000
VERSION     DAEEDS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.241"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..180)
                     /locus_tag="KLH32_004255"
     CDS             complement(<1..180)
                     /locus_tag="KLH32_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBE0140831.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="KLH32_004256"
     CDS             complement(321..875)
                     /locus_tag="KLH32_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE0140832.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="KLH32_004257"
     CDS             complement(872..1162)
                     /locus_tag="KLH32_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE0140833.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="KLH32_004258"
     CDS             complement(1162..1761)
                     /locus_tag="KLH32_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0140834.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa tac
//
LOCUS       DAEEDS010000235         1809 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEEDS010000235 DAEEDS010000000
VERSION     DAEEDS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.242"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            173..1198
                     /locus_tag="KLH32_004259"
     CDS             173..1198
                     /locus_tag="KLH32_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0140835.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="KLH32_004260"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="KLH32_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          506 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAEEDS010000236         1783 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEEDS010000236 DAEEDS010000000
VERSION     DAEEDS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.243"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            53..1783
                     /locus_tag="KLH32_004261"
                     /pseudo
     CDS             53..1783
                     /locus_tag="KLH32_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          468 a          402 c          445 g          468 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccactcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taa
//
LOCUS       DAEEDS010000237         1725 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEEDS010000237 DAEEDS010000000
VERSION     DAEEDS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.244"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..243
                     /locus_tag="KLH32_004262"
     CDS             <1..243
                     /locus_tag="KLH32_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0140836.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="KLH32_004263"
     CDS             complement(332..844)
                     /locus_tag="KLH32_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBE0140837.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..>1725
                     /locus_tag="KLH32_004264"
     CDS             1039..>1725
                     /locus_tag="KLH32_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140838.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGI"
BASE COUNT          506 a          386 c          437 g          396 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgccg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtatt
//
LOCUS       DAEEDS010000238         1724 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEEDS010000238 DAEEDS010000000
VERSION     DAEEDS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.245"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..736)
                     /locus_tag="KLH32_004265"
     CDS             complement(<1..736)
                     /locus_tag="KLH32_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBE0140839.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGTFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRSWNVNVSRVMDNATH"
     gene            complement(736..1101)
                     /locus_tag="KLH32_004266"
     CDS             complement(736..1101)
                     /locus_tag="KLH32_004266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140840.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            1186..1724
                     /locus_tag="KLH32_004267"
                     /pseudo
     CDS             1186..1724
                     /locus_tag="KLH32_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          437 a          435 c          435 g          417 t
ORIGIN      
        1 catgggtggc attatccatc acccgggata cgttgacatt ccagctacgg cggtaatgcg
       61 tgtccaggaa gcgattccat tcccgtttac tgcttccttc tgctgccagc gcatccggca
      121 tcaccaggtc agggtatttc cgtgacagca accgtgttat ccggtagcgc cacatgctca
      181 tcaccttacg ggcgtaaaaa tgaagatttt tccaggtgtg gcccgacgtc acaccaccgg
      241 cagttgtcga taaatggata tgcggatgcc actgctggtc acgcccccat gtgtggatca
      301 ccgtgaatgt ccccggctcc acatctgcct gatggcagat ttccagtatc acatccgctg
      361 caatgcggct catctctgcc agtaaccacc ggttgtggaa caccagggac cagtactggc
      421 agggaagtgt gaacacaata tgctgccacg ggcagtcggg gaccaggctc agcagatact
      481 gtatccactg tgcgccagcc ttcaccccgc agtgcgggca ggagcggctt ttacaccgga
      541 agcagacctt ttttgtatgg caacagtccg gtgatgaaca acaccactgc gtgtagccca
      601 tcagtgtggt cccgcaggcc atgatttttg tcactgattc aattaccacc ggacggactg
      661 ccccttccgg ttgtttctcc agccagttaa gccagcggtt tccctgctga aaaatatcgg
      721 caaaacgggg gagcatcaga agggcggggc gactccgtcc ggccagtgaa ccgtgccaca
      781 ctccgggcag tacataccgc cggcgctgat accggcaaga atggtcgcaa attcccgctc
      841 cgtgcagcgg gcgatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag
      901 ctttttgttt cccgcatcca caaagaacgg aatattcagg tctgcgcagc attcaacggc
      961 atcgtcaaaa ctatcaaagc gcagaacttc tgcgtcttct tcgtcaaaaa aatcatcttc
     1021 gtgaagcttc acgacatagc ggggaagttt gcttctttga gaggcgggtt tacgtttacg
     1081 gggtttagct gaacgggcca tataaccacc tgaaagacaa tgacattgcc tgtttttata
     1141 acggtaattg cagaccatga caagccgcag ccgtcaggct gcctactcga taatttaagt
     1201 tcagcagcag ttaaaacagc aggtgtactt atcagctttc aggctgtact atccgcttat
     1261 cagcgagtaa tggaagttgg ccttcaacgt gcaagtgctg aacgttcaat tgatttcgta
     1321 ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca aacaacgggt
     1381 ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc tagagagtct
     1441 ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc tcgtctgatg
     1501 gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat ggctggtaag
     1561 ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc tggtatggtt
     1621 caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc tatgaagaag
     1681 ggggctttaa aatctgccga tactcttcag aaggtcactc agga
//
LOCUS       DAEEDS010000239         1706 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEEDS010000239 DAEEDS010000000
VERSION     DAEEDS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.246"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..87)
                     /locus_tag="KLH32_004268"
     CDS             complement(<1..87)
                     /locus_tag="KLH32_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBE0140841.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLD"
     gene            complement(768..842)
                     /locus_tag="KLH32_004269"
     tRNA            complement(768..842)
                     /locus_tag="KLH32_004269"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(807..809),aa:Gly,seq:tcc)
     gene            complement(846..920)
                     /locus_tag="KLH32_004270"
     tRNA            complement(846..920)
                     /locus_tag="KLH32_004270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(885..887),aa:Thr,seq:tgt)
     gene            complement(923..999)
                     /locus_tag="KLH32_004271"
     tRNA            complement(923..999)
                     /locus_tag="KLH32_004271"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(963..965),aa:Arg,seq:tct)
     gene            complement(1001..1077)
                     /locus_tag="KLH32_004272"
     tRNA            complement(1001..1077)
                     /locus_tag="KLH32_004272"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1041..1043),aa:Ile,seq:cat)
     gene            complement(1267..>1706)
                     /locus_tag="KLH32_004273"
     CDS             complement(1267..>1706)
                     /locus_tag="KLH32_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE0140842.1"
                     /translation="GKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRD
                     MINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELLN
                     DAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          468 a          451 c          348 g          439 t
ORIGIN      
        1 atccaaccac tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc
       61 tgttgatatt ttttccatct gatacatatc ccgccccccc gacagacctg tgctatcgga
      121 aagaaaaaag gccatcagca aaactctgat ggcctgaatc acctttacca atattgtatg
      181 aaaaaacacg cacgactaat tgacaataat tttcatttcc attaaaaaag atcgcgtagc
      241 attcttaatt catgaggaac tttacccgcc agcaatctga gtagcgaaag ctgttcatcc
      301 ccaatgtttt tgctggcggg tccttttttc tacggtcctc ctccccggag ggggcatttc
      361 attatttttc taatcgttac tggtgagaag gcggaagaaa aagccagttc ttcgccccta
      421 aataacagca tgattccagt ccatgcaaaa tctgatttcc agctcacaca taccgagcac
      481 tgtacaaaaa accgccaacc aaggcagtta agagtgtgtt cccggggttt gcttagaata
      541 ttttgtaagt tgtccgaaga gatatttaca acaccagaat gatgcattat cagtcccctg
      601 ccagaggttc actacgtaac ctgaccgaca atgcaccatt ctggtgctga aaaaagagca
      661 ctggaactgc aacaaaacaa catcacagaa caggagatgg taaggagtca gacattccca
      721 cacaatattg tgtcagtgca aataacaacc ttcgtctcaa atctcgctgg agcgggcagc
      781 gggaatcgaa cccgcatcat cagcttggaa ggctgaggta atagccatta tacgatgccc
      841 gcatatggtg cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgccct
      901 gcctgctgag ctaagtcggc gctggcccac caccgaggac tcgaacctcg caccgtcaac
      961 ttagaaggtt gatgctctat ccggatgagc tagtggtggt tggtggccct tgctggactt
     1021 gaaccagcga cctggcgatt atgagtcgct cgctctgacc aactgagcta aagggccgga
     1081 ggcagaataa taaccatatg tcatcacatc tgcaaactca tctgatcacc agcacgttta
     1141 acgtcctgtg ccgtttttca ggtataaaaa aaaccgcata aagcgggctc tttcaaatgt
     1201 ccatgtctgc tattcgcctc gcggtacagc tttgcgaagc gtacaagaat tgaagcagtt
     1261 tttacgtcaa aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta
     1321 caaaataaat tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact
     1381 ccatttcggg tgtgcatcat tcagcagttc agccattttg catttggtca tcccccgccc
     1441 ctcataccgt tgccgaagga cgctaatcaa tcctggatgc tctgccagca cctcacttat
     1501 gacccgatca atacataacg cctctgcatc agtacaatgc gccagccagc tcttttgctt
     1561 accgttaatc atatcccgca aaaaagcctc aagttcagac ttgttcagac ctgctttttt
     1621 catcctccgg agcgcctcgt taattgccgt ttttgtcagc tttttagagg tcaacatcag
     1681 gttaaacata ttccccgtct taccgc
//
LOCUS       DAEEDS010000240         1656 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEEDS010000240 DAEEDS010000000
VERSION     DAEEDS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.247"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(570..749)
                     /locus_tag="KLH32_004274"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="KLH32_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            798..1656
                     /locus_tag="KLH32_004275"
                     /pseudo
     CDS             798..1656
                     /locus_tag="KLH32_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          431 a          340 c          450 g          435 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgctgcc atgaagcaga
     1081 ttaaagaact ccagcgcctg ctcggcaaga aaacgatgga aaatgaactc ctcaaagaag
     1141 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
     1201 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
     1261 cagacgaacc gatgactgga aagatggtcg ccgcagccgt cactcagatg atacggatgt
     1321 gcttctccgt atacaccatg ttatcggaga gctgcccaca tatggttatc gtcgggtatg
     1381 ggcgctgctt cgcagacagg ccgaacttga tggtatgcct gcgatcaatg ccaaacgtat
     1441 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaactcgctg taccgccatc
     1501 gaaacgggca cataccggca gagtggctgt gaaagaaagt aatcagcgat ggtgctctga
     1561 cgggttcgag ttccgctgtg ataacggaga aaaactgcga gtcacgttcg cgctggactg
     1621 ctgtgaccgt aaggcactgc actgggcggt cactac
//
LOCUS       DAEEDS010000241         1600 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEEDS010000241 DAEEDS010000000
VERSION     DAEEDS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.248"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            154..477
                     /locus_tag="KLH32_004276"
     CDS             154..477
                     /locus_tag="KLH32_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBE0140843.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="KLH32_004277"
     CDS             474..890
                     /locus_tag="KLH32_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBE0140844.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="KLH32_004278"
     CDS             856..1380
                     /locus_tag="KLH32_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140845.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1600
                     /locus_tag="KLH32_004279"
     CDS             1377..>1600
                     /locus_tag="KLH32_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140846.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          391 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagcaa
//
LOCUS       DAEEDS010000242         1597 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEEDS010000242 DAEEDS010000000
VERSION     DAEEDS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.249"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..636)
                     /locus_tag="KLH32_004280"
     CDS             complement(<1..636)
                     /locus_tag="KLH32_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBE0140847.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="KLH32_004281"
     CDS             complement(750..1322)
                     /locus_tag="KLH32_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0140848.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          318 g          468 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAEEDS010000243         1538 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEEDS010000243 DAEEDS010000000
VERSION     DAEEDS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.250"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(910..1386)
                     /locus_tag="KLH32_004282"
     CDS             complement(910..1386)
                     /locus_tag="KLH32_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0140849.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          346 a          410 c          386 g          396 t
ORIGIN      
        1 aaaaaaacaa cagcttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tcctggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAEEDS010000244         1506 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEEDS010000244 DAEEDS010000000
VERSION     DAEEDS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.251"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(66..611)
                     /locus_tag="KLH32_004283"
                     /pseudo
     CDS             complement(66..611)
                     /locus_tag="KLH32_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1266..>1506)
                     /locus_tag="KLH32_004284"
     CDS             complement(1266..>1506)
                     /locus_tag="KLH32_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140850.1"
                     /translation="GVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVV
                     DVLATGSVKVKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          440 a          321 c          316 g          429 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcagaattc catccagtcg ttttgggttg ctaatgatgt cctgcatccg cttaatctgg
      121 tcgggcgtag acatataacc ccacagggtg agcagaacct tttttgcggc tacactattt
      181 ccttggtctg atactaatgc catatagccc cataaactat ccctcagcca gacagcatca
      241 tagtgggttt ctgccgctgt tttgtcatct tcgaaagtag aagcaagaat aaacgttccc
      301 cagggtttct ccgtcaacgt taactttcgt gtgttttctg ttaatgttag caatccttga
      361 gtttcgtctt tggtatagtg actctttatt tcatttttta atttggctga gtgaatcaga
      421 agacgataat taggatactt atctggattt aacgataaat aacttttatc tgttgctgcc
      481 cccctggaat taagaatgtt aaatacagat tcgcttgtca cttctgcctc tgtcgcctgg
      541 catgtgccgc acgaaaatat accagcgagc agaagagtta atgcagattt ccctttcata
      601 aaataatcca tcaatcgttt gttaaaagcg tagataacca cttattcaac aacttgaaaa
      661 taaggtattt tacctgggtt gttacaaaag gattgcattg cgtaaacgct ttttatttac
      721 aaaaaaatgg ggaagtgtta cagcgattgc gaaaagcctg aagtgattga attaagataa
      781 tatagaaaat cagatacagc aaaaatggca gattgatcag gtgaagataa attggagcgg
      841 gcgaagggaa cctaggtatc gagggtatcg aagataatca gccgctaacc atttgtttcc
      901 ttaaaggatg atgaatcctt gtggttacag ttatggttac acattaattt atctctggtc
      961 aagtcccgaa gtcctaactt taagacagtg gccccaatcg tccagacttg aaaggggcct
     1021 atgggggaag tcgctgacct ttatctatta aatacctaca gagatttttg tgagattttt
     1081 taatccaagg tcataaccta ctgcccccac aggccgcaaa gtgaagacct gtaagcacca
     1141 gagcaatagc gagtatctat cactccctgt agagtatctg gtagaccaag aggacacaga
     1201 ggacgaccat atacaactcc tgtaagctca tggatgtact ccttatgttc actcagggtt
     1261 tatagttact ctgcggcagt aaaacgaata acatggacca acgggaaaga ggcgtcttcc
     1321 tcaacgtcaa actcacctgc tttcacctta acggaaccag tagccaacac atcgaccaca
     1381 ctgattaact caaggtcata cccagcagtg ttgctatttc gggtcacttt gacatctacc
     1441 gtcttagcgt ctttagttga cctgaaagag atagaagcga accgcttagg caggagatag
     1501 acacct
//
LOCUS       DAEEDS010000245         1494 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEEDS010000245 DAEEDS010000000
VERSION     DAEEDS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.252"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            67..850
                     /locus_tag="KLH32_004285"
                     /pseudo
     CDS             67..850
                     /locus_tag="KLH32_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            857..1494
                     /locus_tag="KLH32_004286"
                     /pseudo
     CDS             857..1494
                     /locus_tag="KLH32_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          414 a          337 c          427 g          316 t
ORIGIN      
        1 cgtgccggat tgttttgttc ggtatcagtt aaatgtaacg ctgcgagcgt tacaaggtga
       61 aaacaaatgg cttcaaactg gataaagctc gaggttatta cgccggataa gccggaaata
      121 ttcaggcttg ctgagattct gaatattgat ccagatgccg cattagggaa ggttattcgc
      181 ttctgggcat gggcggatca acaaatgata gacggtaatg cagattgtaa cgctcgcggc
      241 gttacaaaaa gtgcaataga tcgcatcact tttatgtctg gttttgctga tgcgttaatt
      301 caggttggat ggctggtcga aaatgacggt gggctttctc tacctaactt tgaacgtcat
      361 aacggaaaaa gctctaaaaa acgggcggtt acaaacgagc gagtaacaaa aatacgcgaa
      421 ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc gttaccagtg
      481 gaagaggaag aggaagagga agatctaaat actgatctcc ccctaaatcc ccctcgccaa
      541 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
      601 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
      661 cagggcgata cgggaactgg aaaaattccg tcagcagggt tttacacctg agcaggtgat
      721 tcgacacagc atcgccaatg aataccaggg cctgttcgcg ccgaaaggtg ttcggcctga
      781 gacgttgctc cgacaggtta acaccgtctc gttgccggac agtgcgatcc cgccaggctt
      841 cagggggtaa cggaccatga aaaatattgc gacaggcggc gttctggaac gcatccgccg
      901 actgaccccg caacatgtaa ccgccccatt cagaacggta gcggagtggc gcgagtggca
      961 acttgctgaa ggccagaaac gtagcgagga gatcaaccgc ctgaatcgcc agttgcgggt
     1021 ggaaaaaatt ctgaatcgct caggcatcca gccgttgcac cgtaaatgct cgtttgcgaa
     1081 ttaccaggtg cagaacgacg gccagcgata cgcgttaagc caggcgaaat ccatcgccga
     1141 tgaactgatg accgggtgta caaattttgc gttcagcgga aaacctgata ccgggtagaa
     1201 tcacttagcg gcagctatcg ggaatcgcct gctgaaagac ggtcagacag tgattgtggt
     1261 taccgtggct gatgttatga gcgccctgca cgccagctat gacgatgggc agtcaggcga
     1321 aaaatttttg cgggagctgt gcgaagtgga tctgctggtt cttgatgaaa ttggcattca
     1381 gcgcgagacg aaaaacgagc aggtggtgct gcaccagatt gttgatcgcc ggacagcgtc
     1441 gatgcgcagc gtggggatgc tgacaaacct gaactatgag gtgatgaaaa catt
//
LOCUS       DAEEDS010000246         1450 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEEDS010000246 DAEEDS010000000
VERSION     DAEEDS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.253"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..656
                     /gene="ipaH9.8"
                     /locus_tag="KLH32_004287"
     CDS             <1..656
                     /gene="ipaH9.8"
                     /locus_tag="KLH32_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBE0140851.1"
                     /translation="VAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSL
                     GREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGV
                     TANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYP
                     QRVADRLKASGLSDDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            complement(657..836)
                     /locus_tag="KLH32_004288"
     CDS             complement(657..836)
                     /locus_tag="KLH32_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140852.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPPGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(836..>1450)
                     /locus_tag="KLH32_004289"
     CDS             complement(836..>1450)
                     /locus_tag="KLH32_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE0140853.1"
                     /translation="SLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRD
                     QAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRTE
                     QEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRDVMTWGDADNQQVKLSMPEL
                     EELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT          328 a          416 c          376 g          330 t
ORIGIN      
        1 ctgttgctgc tgatgccact gagagctgtg aggaccgtgt cgcgctcaca tggaacaatc
       61 tccggaaaac cctcctggtc catcaggcat ctgaaggcct tttcgataat gataccggcg
      121 ctctgctctc cctgggcagg gaaatgttcc gcctcgaaat tctggaggac attgcccggg
      181 ataaagtcag aactctccat tttgtggatg agatagaagt ctacctggcc ttccagacca
      241 tgctcgcaga gaaacttcag ctctccactg ccgtgaagga aatgcgtttc tatggcgtgt
      301 cgggagtgac agcaaatgac ctccgcactg ccgaagccat ggtcagaagc cgtgaagaga
      361 atgaatttac ggactggttc tccctctggg gaccatggca tgctgtactg aagcgtacgg
      421 aagctgaccg ctgggcgcag gcagaagagc agaagtatga gatgctggag aatgagtacc
      481 ctcagagggt ggctgaccgg ctgaaagcat ccggtctgag cgatgatgcg gatgcggaga
      541 gggaagcagg agcacaggtg atgcgtgaga ctgaacagca gatttaccgt cagctgactg
      601 acgaggtact ggccctgcga ttgtctgaaa acggctcaca actgcaccat tcataatcac
      661 atcgcataaa ccacagaccg gactgactcc ggaaaaacag aggcccgccc ccgggcctcc
      721 ccggattcat ccgtttccct gttcagcctg acagcacgcc ccggcggccg gatgacagac
      781 tccgcttcgg taagcaaagc ggtcttctgt gattccgcca gttgcggctt attcattact
      841 caacgtcaaa cgcccgaatt gaagccaaat catccagacc gctcagctcc tctttcattt
      901 cacgctgacg gcgataaatc tcatcgttgc gatcgacctg cgcctgcacc attgctgccg
      961 ccagttcttc cagttccggc atcgacagtt tcacctgctg attatcggca tcaccccacg
     1021 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
     1081 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
     1141 cctgttctgt gcgccatgct tcaatttcct gacgtctggc ctctctggcc gcttccagca
     1201 tttctggtgt cacagtgaat ggggctatct caccccattt gccactttgc agttcctgcc
     1261 agattcgctg acccgtcggt gcgacatcat cagcggtggc tgtgtaggga actgcctggt
     1321 ccctgtcgtc aaaaaaaacg tcacagtcta ctgcgccact ttcggtataa cggggattaa
     1381 tgattttttt aatttccacg gtgcattcct cacgatgtgc gaataaaaag cccgggcatt
     1441 gcgccagaga
//
LOCUS       DAEEDS010000247         1415 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEEDS010000247 DAEEDS010000000
VERSION     DAEEDS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.254"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            15..488
                     /locus_tag="KLH32_004290"
     CDS             15..488
                     /locus_tag="KLH32_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0140854.1"
                     /translation="MAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAEL
                     GDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSP
                     GSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            491..1036
                     /locus_tag="KLH32_004291"
     CDS             491..1036
                     /locus_tag="KLH32_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0140855.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITTNRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPVSPEGSS"
     gene            1060..>1415
                     /locus_tag="KLH32_004292"
     CDS             1060..>1415
                     /locus_tag="KLH32_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE0140856.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          396 a          304 c          379 g          336 t
ORIGIN      
        1 gctttcagaa tggagtggct attgctttta agaatattca ggccggaact gccagaaaat
       61 tcacgttatc cagcgccaac aactccacga aaaatgcagc gttttatttg tggggtaatc
      121 catccaggcc tgttgttgca gagcttggtg atgattcagg ctggcatttt ttcagccaga
      181 gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg ttaaattacg
      241 gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta cggtatggcc
      301 atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc gcaccttcgg
      361 gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg gataacgttg
      421 acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat aacgtagcga
      481 gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa cagcagaaca
      541 atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg gaaaaaactg
      601 gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca ccggagatgg
      661 tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg ccagcgttat
      721 cgaagttccg gatattacca ctaaccgacg gattaccgcg ccgggttact ggttttaccg
      781 caatgatgaa tttgtctttg actacagact caaagcggaa gatgagcgtg atgcccttct
      841 ggctcaggtc agtgcccgga caggggaatg ggaagaagac ctgctgctgg ggttaatcag
      901 cgacgaagat aaagaaaagc tgaaagcctg tcgtatttac gcgaaatcgc tgcaggcgat
      961 ggatttcagc accatcactg ataaatcctc atacaacgcc attgaatggc ccgtctctcc
     1021 ggaaggttct tcctgattta atttatcgcg agaaaaacaa tgtctgtagt gatatcaggt
     1081 gcgctgactg atggcgcagg tatccccatg tccggatacc atattattct gaaatcccgg
     1141 gtaaacaccc cggaagtggt gatgaacact gttgctgatg tgatggcagg aaacgatggt
     1201 gaatactgtt tccatgcgcg gactggaaaa tatggtgtgt atctgaaaca ggactggcgc
     1261 aacgagtaca acgttggcga cattgctgta tatgaggact caaagcccgg cacgctgaat
     1321 gactttctga ttgctcctga tgagggcgac ctgaaaccgg atgtcgtcaa acgctttgag
     1381 gaaatggtgg cgcaggcgca gcagagcgcc ggggc
//
LOCUS       DAEEDS010000248         1380 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEEDS010000248 DAEEDS010000000
VERSION     DAEEDS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.255"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..253
                     /locus_tag="KLH32_004293"
                     /pseudo
     CDS             1..253
                     /locus_tag="KLH32_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1380
                     /locus_tag="KLH32_004294"
     CDS             746..>1380
                     /locus_tag="KLH32_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0140857.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          338 a          320 c          421 g          301 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tccgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
//
LOCUS       DAEEDS010000249         1360 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEEDS010000249 DAEEDS010000000
VERSION     DAEEDS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.256"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..153
                     /locus_tag="KLH32_004295"
     CDS             <1..153
                     /locus_tag="KLH32_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140858.1"
                     /translation="LSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREK
                     YHQMAA"
     gene            complement(197..1246)
                     /gene="cadB"
                     /locus_tag="KLH32_004296"
                     /pseudo
     CDS             complement(197..1246)
                     /gene="cadB"
                     /locus_tag="KLH32_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          419 a          346 c          298 g          297 t
ORIGIN      
        1 ctgagccact atcgttttaa taaccgggat gaagccatct cagtaatacg ggaatacatt
       61 gagattttct acaatcgtca gcgtcgtcac tctcgtctgg ggaatatctc cccggcagcc
      121 ttcagggaaa aatatcatca gatggctgct taaaaaaaga acaaatggta gtgtccgcta
      181 ttgccagtac acctcacagg tcagatgctt taccaccggc agagttcatc agagtgatga
      241 ggatcatcag ggcagtcatt ttcactgcag ccagcagcag accttttttc ggaataccgt
      301 tgctgtcgac ttcaccataa actttcggga agttaccgtc gttagcggca cgtacacctg
      361 cctggcctac caacatcatc taggagccca gagaagtcag gcacgcaaag gcggtgaatg
      421 cagaaaccag cggcgcagcc cagttaccga ggatagctga agcactgatt gcaaacggag
      481 caccggaagc cgccattaca gaagacggat acataccgga aagcacctga gtcgcagcga
      541 tataaacaat acctgctaaa ccagtaccca gcatggttgc cagcggaacg gtacgtttcg
      601 ggtttttaac cataccagta cttactgctg cggattcaac acccacgaag gcccacaggc
      661 agagcagaat acttttaatg atcgcatgac catcagtggt atccgcagta ttccagttag
      721 ctgcataagt tgccgcatca aaccaatgcc agccaacaat agcagtcatc accacaggaa
      781 taagaaccag caccagacca atagtggtta aacggcttac ccaggtaccg ccgagcatat
      841 ttacaaaggt aaatacccag acgatagcaa tacaggcgat acccgccgga acaggatcat
      901 ttaatactgg gaagaaggtg gaaagataag atacagcggt aataccaatc gccaggttac
      961 caatccagtt agcatggtaa taaagaacac ctgtctgaaa accaaatgca ggggaaattt
     1021 ctccggcata agcaattggg ccaccttgtt gcgggttttt tgttgccagt cgggcatata
     1081 catacgccag cgacattgca ccaataatag agataatcca accccagata gcaataccac
     1141 cgatacttgc taggttcgca ggtaataatg caataccgct ccccatcata ttaccggcaa
     1201 caacaccggt acaggcaaat agcccgatct tcttggcaga actcatgctc ttctcctaat
     1261 ttcatttttg aatttggagt ccgggtcatg atgtataact atttcctgac cagaccaaac
     1321 tggcgataag attactcacg aaaaaaggat taatcctaaa
//
LOCUS       DAEEDS010000250         1356 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEEDS010000250 DAEEDS010000000
VERSION     DAEEDS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.257"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(41..100)
                     /locus_tag="KLH32_004297"
     CDS             complement(41..100)
                     /locus_tag="KLH32_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0140859.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="KLH32_004298"
     CDS             complement(339..1247)
                     /locus_tag="KLH32_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140860.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            complement(1281..>1356)
                     /locus_tag="KLH32_004299"
     CDS             complement(1281..>1356)
                     /locus_tag="KLH32_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140861.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccctccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAEEDS010000251         1354 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEEDS010000251 DAEEDS010000000
VERSION     DAEEDS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.258"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..226)
                     /locus_tag="KLH32_004300"
     CDS             complement(<1..226)
                     /locus_tag="KLH32_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0140862.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTEGGDGGLTTAERQRLKE"
     gene            303..845
                     /locus_tag="KLH32_004301"
     CDS             303..845
                     /locus_tag="KLH32_004301"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140863.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            1072..>1354
                     /locus_tag="KLH32_004302"
     CDS             1072..>1354
                     /locus_tag="KLH32_004302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBE0140864.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          373 a          284 c          330 g          367 t
ORIGIN      
        1 gctctttcag acgctgacgt tcagcggtgg tgagccctcc atcaccgccc tcggtatccc
       61 gctcatgctg gcgaacccag acacgcagag tctccggcgt acagccaatc tttggggcaa
      121 tggaacaaat tgtcgcccat tgtgagtcat attcgccctg actttccaga accatacgga
      181 ctgcccgttg acggacttcg ggggaaaaac gagtattttt agtcatcctg tttacctctt
      241 tctcaggaag tttagtctcc aggattcccg gggcggttca ggataacacc aaaggcgatg
      301 ccatgattgg cgtcaagcag cctttaccaa aagcagtttt aagaactcag catgacaaaa
      361 ataaagaagc aataagtatc ctggattttg gtgttattga tgatggtgtg acagataatt
      421 accaggcaat acaaaatgct atagatgccg ttgcttcact accctccggc ggggagctgt
      481 ttatccctgc gagcaaccaa gcggtggggt atattgttgg atccactttg cttattcctg
      541 gcggtgttaa catcagagga gttggtaagg catcgcaact ccgagcaaaa agcggactta
      601 caggatctgt gttaaggctg tcttatgatt cagacactat cggccgttat ctgagaaata
      661 tacgagtaac tggtaataac acctgcaatg gtattgacac aaacattaca gcagaagact
      721 ctgtcatcag acaggtttat ggctgggtat ttgataatgt aatggtgaat gaagttgaaa
      781 ccgcattatt taatgcaagg gctctggcac tcaaaattta tagcatgtca ggctggaacc
      841 tgtagagtcg gtcttcactt tttaggccag tgcgtaagtg ttagtgtcag ctcctgccat
      901 ttcagcagag gaaattattc tgctgatgaa agctttggca tcaggattca gcctcaaacg
      961 tatgcgtggt cgtcagaggc agttaggtca gaagcaataa ttttagacag tgagaccatg
     1021 tgcattggtt ttaaaaatgc cgtctatgtt catgattgcc ttgatttgca tatggaacaa
     1081 ctggatttag attattgcgg ctcaacaggc gtggttatag agaatgtaaa cggaggattt
     1141 tctttctcaa actcatggat agcagcagat gccgatggca ctgaacaatt tacggggata
     1201 tattttagaa cgccggcctc aacgcagtca cataaaattg tcagtggtgt tcatatcaac
     1261 actgcaaaca aaaacacggc tgcaaacaat caaagtatag cgatagaaca gtcggcgatc
     1321 ttcgtctttg taagtggttg tacgttaact ggtg
//
LOCUS       DAEEDS010000252         1313 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEEDS010000252 DAEEDS010000000
VERSION     DAEEDS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.259"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..199
                     /locus_tag="KLH32_004303"
     CDS             <1..199
                     /locus_tag="KLH32_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140865.1"
                     /translation="WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSF
                     SKSVELHDKVIGHYLNIKHYQ"
     gene            700..>1313
                     /gene="uacT"
                     /locus_tag="KLH32_004304"
     CDS             700..>1313
                     /gene="uacT"
                     /locus_tag="KLH32_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBE0140866.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKG"
BASE COUNT          327 a          303 c          291 g          392 t
ORIGIN      
        1 ctggccgctg tatgaatcac gcctgaaggg aaagctgcac gttatcagca agcgttacac
       61 tcagcgcatt gagcgacata atctgaatct gagacaacat ctggcaaggc tgggacggaa
      121 gtcactgtcg ttctcaaaat cggtggagct gcatgacaag gtcatcgggc attatctgaa
      181 cataaaacac tatcagtaag ttggagtcat taccgcatga cagcctatac attccgcagc
      241 ttcagcagcg ataaacttat tcatttgcat ccctttcatt taatgagtta tgtcttctca
      301 aatttcgcga tgcaataaac gggcatatat taggtagcat gaccgtttta attataattt
      361 tgcgactgag ttcaaattat tgcccctcaa tctatgccgc tttcattatt tcaacattgc
      421 cgatcaaaag agcgctatcc agataaaaac gattaaatgc gagagtgcga catgccagaa
      481 tgattaatga aatatcaata tgagaaaatt acagataatt ccattgtcag gttctcttgt
      541 cattttcgtg gtttaagcac gattatgtga ttgcctccgc aattcctgtc tctaactccc
      601 cttcctcgca aaaactggca ctccacgagc atgtgtttag acagtttcat taacgtaaac
      661 ggttgctttt tattctggcg ggcgaaagga gaaacactga tgagcgccat agattcccaa
      721 cttccctcat cttctgggca agaccgccca actgatgagg ttgaccgcat attatcacca
      781 ggaaagctga tcatactcgg tctgcaacac gtccttgtca tgtacgcagg tgcagtcgct
      841 gttcctctta tgattggtga ccgactgggc ctctcaaaag atgctattgc gatgctcatt
      901 agctcggatc tcttttgctg cgggatcgtc acattattgc aatgtatcgg tatcggccgc
      961 tttatgggga tccgcctgcc ggtgattatg tcggtgacct ttgctgctgt aacaccaatg
     1021 atagccattg ggatgaaccc ggatatcggc ctgctgggga tatttggtgc cactatcgcc
     1081 gcgggtttta tcaccacatt attagcgcca cttattggtc gcttgatgcc tttattcccg
     1141 ccactggtta ccggtgtggt tattacttct attgggctta gcatcattca ggtgggtatt
     1201 gactgggccg ctggaggtaa agggaatccg caatatggta atcccgttta tttaggtatc
     1261 tcctttgccg tcttaatttt tatcttgctc attactcgct atgcgaaagg att
//
LOCUS       DAEEDS010000253         1295 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEEDS010000253 DAEEDS010000000
VERSION     DAEEDS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.260"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..869)
                     /locus_tag="KLH32_004305"
     rRNA            complement(<1..869)
                     /locus_tag="KLH32_004305"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          279 a          381 c          283 g          352 t
ORIGIN      
        1 cgcgttagct ccggaagcca cgcctcaagg gcacaacctc caagtcgaca tcgtttacgg
       61 cgtggactac cagggtatct aatcctgttt gctccccacg ctttcgcacc tgagcgtcag
      121 tcttcgtcca gggggccgcc ttcgccaccg gtattcctcc agatctctac gcatttcacc
      181 gctacacctg gaattctacc cccctctacg agactcaagc ttgccagtat cagatgcagt
      241 tcccaggttg agcccgggga tttcacatct gacttaacaa accgcctgcg tgcgctttac
      301 gcccagtaat tccgattaac gcttgcaccc tccgtattac cgcggctgct ggcacggagt
      361 tagccggtgc ttcttctgcg ggtaacgtca atgagtaaag gtattaactt tactcccttc
      421 ctccccgctg aaagtacttt acaacccgaa ggccttcttc atacacgcgg catggctgca
      481 tcaggcttgc gcccattgtg caatattccc cactgctgcc tcccgtagga gtctggaccg
      541 tgtctcagtt ccagtgtggc tggtcatcct ctcagaccag ctagggatcg tcgcctaggt
      601 gagccgttac cccacctact agctaatccc atctgggcac atccgatagc aagaggcccg
      661 aaggtccccc tctttggtct tgcgacgtta tgcggtatta gctaccgttt ccagtagtta
      721 tccccctcca tcgggcagtt tcccagacat tactcacccg tccgccactc gtcagcgaaa
      781 cagcaagctg tttcctgtta ccgttcgact tgcatgtgtt aggcctgccg ccagcgttca
      841 atctgagcca tgatcaaact cttcaattta aaagtttgat gctcaaagaa ttaaacttcg
      901 taatgaatta cgtgttcact cttgagactt ggtattcatt tatcgtcttg cgacgttaag
      961 aatccgtatc ttcgagtgcc cacacagatt gtctgataaa ttgttaaaga gcagtaccgc
     1021 ttcgcttttt ctcagcggcg cggggtgtgc ataatacgct ttcccgctac agagtcaagc
     1081 atttattttt gctttctctg ccggaattct caggagaacc ccgctgaccc ggcggcgtgt
     1141 ttgccgttgt tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagcg
     1201 aaaaatataa aaactttatc gttcgctcac ttttcaggca aaacatctta aatatagtct
     1261 tttccgtcta acttatagac aaaaacgagc cccga
//
LOCUS       DAEEDS010000254         1259 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEEDS010000254 DAEEDS010000000
VERSION     DAEEDS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.261"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..151)
                     /locus_tag="KLH32_004306"
     CDS             complement(<1..151)
                     /locus_tag="KLH32_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0140867.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARK"
     gene            217..987
                     /locus_tag="KLH32_004307"
                     /pseudo
     CDS             217..987
                     /locus_tag="KLH32_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1008..>1259
                     /locus_tag="KLH32_004308"
     CDS             1008..>1259
                     /locus_tag="KLH32_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE0140868.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQS"
BASE COUNT          386 a          281 c          283 g          309 t
ORIGIN      
        1 cttttctggc ggcggtaacc cattgtccta aagtgccttc aggaagagat aatcgggaag
       61 cgccttcact gatcgaaagt tgattttcaa gaaccgttct gacagcttcg gctttgaact
      121 ctttagagta acgttgggtt tttctgctca ttattagctc cttctgatgc cattctattt
      181 caggaaggag tgtccgttaa actcaggcta cctcagtttc aatttgcaac agataatggt
      241 tatacatcag gaaatccatt taacggtctg acaccattaa aaaagtccaa gatagaacca
      301 gatcccctca cccgtgacga atttattcgt tttattgagg cttgccgtca tcaacaaaca
      361 aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc acggggagct ggtatcactg
      421 gcctgggaag atatagacct taaagcgagg actataacca tccgccggaa ttatacaaaa
      481 ctaggcgaat tcactccacc aaaaactgat gctggtaccg gaagaacgat tcatctagtt
      541 caaccagcta ttgatgctct taaaagccaa gcggaaatga ccatgcttgg aaaacagcat
      601 tctgtagagg taaagcagag ggaatatggg agaagtactg tgcataaatg cacttttgtt
      661 tttagtcctc aggtaataaa acagcggcag ttttccggac cgcactataa ggttgactcc
      721 atcagggagt catggacaag tatcttaaaa cgcgcaggtc tgagacacag aaaatcgtat
      781 caatccaggc atacctatgc atgctggtca cttgccgcag gagctaatcc tagttttatc
      841 gcaagccaga tgggccacac aaacgcacaa atggtattca atgtttacgg agcatggatg
      901 aaagacaaca atcacgaaca gatagaactt cttaacagaa gactatctga aagtgtccca
      961 tgcatgcccc ataagaaacc ggggtaaaat aaaaacttgc aaaatcaatt ggtttacctt
     1021 taatccctgt cacgttacgc gcgtggcaga ggcgttacgg attgctgaaa ccgcaacgga
     1081 cagacggcgg tcatcggctg ttcaacgatg ccgatattga ccggatccgc gagatcaaac
     1141 gctggatcga caacggcgtg caggtcagca aagttaaaat actgctcagt aatgaaaatg
     1201 ttgatgtgca gaacgtctgg cgcgatcagc aagaaacatt actgacttac ctgcaaagc
//
LOCUS       DAEEDS010000255         1252 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEEDS010000255 DAEEDS010000000
VERSION     DAEEDS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.262"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..167)
                     /locus_tag="KLH32_004309"
     CDS             complement(<1..167)
                     /locus_tag="KLH32_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140869.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGKHQK"
     gene            complement(282..422)
                     /locus_tag="KLH32_004310"
     CDS             complement(282..422)
                     /locus_tag="KLH32_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140870.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            561..1232
                     /gene="queE"
                     /locus_tag="KLH32_004311"
     CDS             561..1232
                     /gene="queE"
                     /locus_tag="KLH32_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBE0140871.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          362 a          258 c          299 g          333 t
ORIGIN      
        1 attttctggt gcttaccggg ctgagaggcg gtgtaagtga actgtagttg ccatgtttta
       61 cggcaatgag agcagagata gcgctgatgt ccggcagtgc ttttgccgtt acgcaccacg
      121 ccttcagtag cggagcagga aggacatctg atggaaatgg aagccacgca agcaccttaa
      181 aatcaccatc atacactaaa tcagtaagtt ggcagcatta cccaacaaag gaacatacga
      241 aagtgaacat tagtatgaaa tagcgcatta taaatttctc attaaatctg gataaataaa
      301 ttgacggcgg catcggtaat tttagattcg ttatcattaa aaactaatac agaaaaaata
      361 aatataattt tggtgaatgt tttgacatga ttaaatccat tttctttatt ttcttctgac
      421 ataatacgtt actcccttta attattaacg atatgtttcg tatttttctt taaatctgca
      481 tgtaacagaa tgttaataag acatgattgt tgcggcagga tctgcaataa ttaacaatta
      541 tccacttcac agagaatgct atgcagtacc cgattaacga gatgttccag accctgcaag
      601 gtgagggtta ctttaccggc gtccccgcca tttttattcg tttacaggga tgcccggttg
      661 gctgtgcctg gtgcgacacc aaacacacct gggaaaagct tgaggatcgg gaagtctccc
      721 ttttcagcat tctggcgaag accaaagaga gtgataagtg gggggcggcg agcagtgaag
      781 acttgctggc tgtcattggt cgtcagggat ataccgcgcg gcatgtggtg attacgggtg
      841 gtgagccttg cattcatgat ttgctgccac tgactgacct gctcgaaaag aacggtttta
      901 gctgccagat cgaaaccagt ggaactcatg aagtacgctg cacaccgaat acctgggtta
      961 ccgtatcgcc aaagctgaac atgcgcggcg gctatgaagt gttgtcacag gcactggagc
     1021 gagccaacga aatcaagcat ccagtggggc gcgtacgcga tattgaagca ctggatgaac
     1081 tactggcaac gctgaccgat gataaaccgc gagtcattgc actgcagccg attagccaaa
     1141 aggatgatgc cacacgattg tgcattgaaa cctgtattgc gcgtaattgg cgtttgtcga
     1201 tgcaaacaca taaatattta aatattgcct gattacacat ttataagcgt ta
//
LOCUS       DAEEDS010000256         1212 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEEDS010000256 DAEEDS010000000
VERSION     DAEEDS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.263"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(186..260)
                     /locus_tag="KLH32_004312"
     tRNA            complement(186..260)
                     /locus_tag="KLH32_004312"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Gly,seq:tcc)
     gene            complement(264..339)
                     /locus_tag="KLH32_004313"
     tRNA            complement(264..339)
                     /locus_tag="KLH32_004313"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(304..306),aa:Thr,seq:tgt)
     gene            complement(342..418)
                     /locus_tag="KLH32_004314"
     tRNA            complement(342..418)
                     /locus_tag="KLH32_004314"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Arg,seq:tct)
     gene            complement(420..496)
                     /locus_tag="KLH32_004315"
     tRNA            complement(420..496)
                     /locus_tag="KLH32_004315"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Ile,seq:cat)
     gene            complement(705..1211)
                     /locus_tag="KLH32_004316"
     CDS             complement(705..1211)
                     /locus_tag="KLH32_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBE0140872.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          313 a          292 c          299 g          308 t
ORIGIN      
        1 tagtagacgc cgttacgctg gtagagatag tagactgctg gaagtttgtg actactggat
       61 agctgaatca tatgtctgta tgccctgaga gggttacaca gtgggttaca gtaagtaact
      121 ctgcaaggag gcttaaagcc gtatccctta ggattcagta ggttggagta ctttaagaaa
      181 ggtcatggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat
      241 agccattata cgatgcccgc atatggtgcc gactaccgga atcgaactgg tgacctactg
      301 attacaagtc agttgctctg cctgctgagc taagtcggcg ctggcccacc accgaggact
      361 cgaacctcgc accgtcaact tagaaggttg atgctctatc cggatgagct agtggtggtt
      421 ggtggccctt gctggacttg aaccagcgac ctggcgatta tgagtcgctc gctctgacca
      481 actgagctaa agggccggag gcagaataat aaccatatgt catcacatct gcaaactcat
      541 ctgaccacca gcgcgtttaa cgccctgtgc catttttcag gcacaaaaaa acccgcataa
      601 agcgggttct ttcaagtgtc catgtctgct attcgcctcg cgatacagct ttgcgaagct
      661 taccggaatt gaagcagttt atgtgcaaaa atgcaagaac ttttttaaag ctgcatcaac
      721 ctttccacca gtttatctct gcgaacaaca aaccaaccat tggctctcgc cagttccagc
      781 catgactcaa gggaaataac aatatcatca tcccgtaact gaattgtgga aacagtgaca
      841 ccgcctctct gataacagag aactcgcgtg tcgtaacttt tctggcatga aactggcgtt
      901 gacggatccc tttgactgaa atagcagtct tccagttttt cgaacacatc ccacgcctga
      961 tcggtttcga gcattttgcc gtggcgtgct gcgcctcgtt ctgtccagag gatgagggag
     1021 cgggctttgg gtgaaactgg attttgtgag tagtttaaag cgacccgcaa ttctttaagg
     1081 tcattaccaa caactttgaa aaagtgtttc ccttcaacga agcgtacttt gttctcatga
     1141 tgattctggc gaatacgcac cggctcagtg ccgtaaagct gcgccaaaag ttcggtggta
     1201 ataacaggaa tc
//
LOCUS       DAEEDS010000257         1110 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEEDS010000257 DAEEDS010000000
VERSION     DAEEDS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.264"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..1019
                     /locus_tag="KLH32_004317"
                     /pseudo
     CDS             1..1019
                     /locus_tag="KLH32_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAEEDS010000258         1102 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEEDS010000258 DAEEDS010000000
VERSION     DAEEDS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.265"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            52..>1102
                     /locus_tag="KLH32_004318"
     CDS             join(52..301,301..>1102)
                     /locus_tag="KLH32_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HBE0140873.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
BASE COUNT          303 a          273 c          310 g          216 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg ga
//
LOCUS       DAEEDS010000259         1080 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEEDS010000259 DAEEDS010000000
VERSION     DAEEDS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.266"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..307
                     /locus_tag="KLH32_004319"
     CDS             <1..307
                     /locus_tag="KLH32_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140874.1"
                     /translation="LNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVG
                     IWCFAFTACACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(308..952)
                     /locus_tag="KLH32_004320"
     CDS             complement(308..952)
                     /locus_tag="KLH32_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0140875.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          278 a          247 c          256 g          299 t
ORIGIN      
        1 tcttaactcg gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt
       61 cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc
      121 aatgaccgtc gggatttggt gttttgcctt taccgcctgt gcctgtttga cggggatctt
      181 cccgaaaatg gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac
      241 gccgtttgtg ctggtaggat taggactgat attcccgctg ctggcgcgta aagcgaatag
      301 caaataatta ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat
      361 cgagaaatgc gcgcaagccg caggacatat actggcggtt tgggtaatag acttggaagc
      421 ctggacgctc tgtgatccag tcttccagta cacattctaa acgaccagta tcaaggtact
      481 ccttgatctg ttcatagagc agatacccga taccagcacc catcaaaacg gcctccagtt
      541 cagcatcaac atcatccaga atgatatttc ctgcgacagc aatttccagc tcctttgcaa
      601 attgccagtg gaaaggtttg ccgctgggat aacgaaaaac aacacattga tgattaagca
      661 gatcatgagg atggcggggt ttgccatatc ttgcaaagta ctctggcatt gctgctacac
      721 ataatttcac tggtgggccg atcgccacgc tgatcatatc tttttcgacg atgccactaa
      781 gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat aagagaatcg tcagttgtga
      841 gctctacttt aatatcagga tattcacgtg tgaaaccaac cagtaaaggc attaaaaata
      901 tacgtgcagc tacccgggcg gcattgattt tgagcgttcc tgtcggtgtc agacgaaaat
      961 cattcatctc atctaacatg atctgaattt cgtcgaaagc tgggcgcaat cgttcataga
     1021 gattagaacc cgcttccgtt aacgatacgc ttcgggttgt ccgattgaat aagcgaattt
//
LOCUS       DAEEDS010000260         1031 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEEDS010000260 DAEEDS010000000
VERSION     DAEEDS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.267"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>1031
                     /locus_tag="KLH32_004321"
     CDS             <1..>1031
                     /locus_tag="KLH32_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0140876.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAE"
BASE COUNT          319 a          193 c          269 g          250 t
ORIGIN      
        1 tgtctataac cacgaaccgt tatgcatcag gttatgccac actgactgaa gcagtaagtg
       61 cacaggatga acggaacaga aaaagagata agaatagtca cgatggctct acgatatcct
      121 tatcccagcc ccttggtaat atcgggaatt tgaacttcaa taccacgagg tataattctt
      181 cccggggcac aggaaacacg cggtctacca gcctgagtta tagtacggta tggagaggca
      241 taacattctc cattaactgg gcaaaaaacg acttgctgac atcgcataaa tggaaagtag
      301 atcggaaatt aagcgtgggt atttcagtgc cattatcgct gggtgacgaa aatcaaattt
      361 atgcgtcttc acagatgagt cgttcgggag agcagggtaa taactatcag gtttcattat
      421 caggtcagaa tagtggtggt gtatggtggg atgtagcgac aaatataact aacgctcatc
      481 agtcacaacc gaagagcacg atgaacattg tgcaagttgg caaaaatgga tcatatggtc
      541 agtttagtag ccattacagc tccagcgaaa acatgaaaca gctaggtgca aatttaagcg
      601 gtgggatact tattacaagg gatggactta catttggtca aaatgtggat gggacattag
      661 cccttataga ggcacccgga gcaacggggg ttaacgttaa tggctggccg ggactgagta
      721 ctgattttcg tgggtatgca atactgccag tgcagccata ccgaagagac gatgttattc
      781 tggatgaaaa aacgataggt aagaattacg acttaccaca aactagtcaa ttggttgtac
      841 cgacagctgg agccgttgtg cctgcgacat tggcagtgaa gagcggggat aaaggattag
      901 taaccttaaa acagaaagag ggtaaacccg tccccttcgg cgctgtgatt agttatagca
      961 aagacactga aaatatggcc ggcattgtag gtgaagatgg tattgcttat gtttctggct
     1021 tgagtgcgga a
//
LOCUS       DAEEDS010000261          983 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEEDS010000261 DAEEDS010000000
VERSION     DAEEDS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.269"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KLH32_004322"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KLH32_004322"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBE0140877.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAEEDS010000262          933 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEEDS010000262 DAEEDS010000000
VERSION     DAEEDS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.270"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(55..243)
                     /locus_tag="KLH32_004323"
     CDS             complement(55..243)
                     /locus_tag="KLH32_004323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140878.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAEEDS010000263          929 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEEDS010000263 DAEEDS010000000
VERSION     DAEEDS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.271"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            768..>929
                     /locus_tag="KLH32_004324"
     CDS             768..>929
                     /locus_tag="KLH32_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE0140879.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEELN"
BASE COUNT          288 a          207 c          177 g          257 t
ORIGIN      
        1 gttttctcta cttatcaagg gataattaat acgactcact atagggagaa gggacacaaa
       61 aagttttctc ttctaggaca actaaccgta acttatcgca tcctttcagg ggcgggatga
      121 ccaattacag acaccaagcg ttctacttaa agtgaatgct tcctgtgtgc tctgggtcag
      181 tcgataagtg aatagcaata agacacaatc atcttaatta tggtctctag tccctacagg
      241 ggcttaactt aaagttaact taaagacaca attaaagatt aacttatagt attacttaaa
      301 gttaacttaa agacacaatt agagattaac tttaagtatt aaataaaaga tttaattaaa
      361 gaccaattaa agattaactc taagggaata ccttaagggg tgatgatatt tataataatc
      421 aatcaccaaa cctataggtc ttctctaggg tctctctata ggtctctcta gggtctcctc
      481 taggcattac ccccagaagc taactctatg gccctctatg gccctgtacg tcattcttta
      541 ggatgccctg tagtttcgta tagcctgatg cctagagaga ccctagagtg gctcctagag
      601 agaccctccc agtagctccg cacagctaca tcgtcatcat catagtattc aatctcaaag
      661 tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc gcttggggac tttgagtagg
      721 tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa tcaagcgatg tctcaacaac
      781 cacaacaacc acaacaacca caacaagaaa tcagcatcat taacctcgac cagcttgtgt
      841 ccatgacctc agttgaaatt gctgagctga caggcaagga gcaccgaaat gtccttcgtg
      901 atattcgtaa gatgctcgaa gaacttaac
//
LOCUS       DAEEDS010000264          920 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEEDS010000264 DAEEDS010000000
VERSION     DAEEDS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.272"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            8..>920
                     /locus_tag="KLH32_004325"
     rRNA            8..>920
                     /locus_tag="KLH32_004325"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          258 a          197 c          281 g          184 t
ORIGIN      
        1 gttgtgaggt taagcgacta agcgtacacg gtggatgccc tggcagtcag aggcgatgaa
       61 ggacgtgcta atctgcgata agcgtcggta aggtgatatg aaccgttata accggcgatt
      121 tccgaatggg gaaacccagt gtgattcgtc acactatcat taactgaatc cataggttaa
      181 tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa tcaaccgaga
      241 ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag tgtgtgtgtt
      301 agtggaagcg tctggaaagg cgtgcgatac agggtgacag ccccgtacac aaaaatgcac
      361 atactgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat atggggggac
      421 catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg tgagggaaag
      481 gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgtac gtacaagcag
      541 tgggagcacg cttaggcgtg tgactgcgta ccttttgtat aatgggtcag cgacttatat
      601 tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta actgggcgtt
      661 aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt gaaggttggg
      721 taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg acttgtggct
      781 gggggtgaaa ggccaatcaa accgggagat agctggttct ccccgaaagc tatttaggta
      841 gcgcctcgtg aattcatctc cgggggtaga gcactgtttc ggcaaggggg tcatcccgac
      901 ttaccaaccc gatgcaaact
//
LOCUS       DAEEDS010000265          911 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEEDS010000265 DAEEDS010000000
VERSION     DAEEDS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.273"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            69..785
                     /locus_tag="KLH32_004326"
                     /pseudo
     CDS             69..785
                     /locus_tag="KLH32_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          311 a          193 c          227 g          180 t
ORIGIN      
        1 gcggctcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag
       61 gaaagaaaat gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga
      121 acggtaaaag atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc
      181 atgacaagct aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa
      241 cgttcgagga agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg
      301 atgacaaaag ccggatcgga ttctggcttc aacatttcgc aggaatacaa ctaagagaca
      361 ttactgaatc aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa
      421 actggaaact cagggcagaa tcatgcagaa aaaaagggaa acctgttcca gaatacacgc
      481 caaaaccagc gtccgttgca acgaaggcta cgcatctttc atttataaag gccctactaa
      541 gagccgcaga gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa gtgcctcaac
      601 caaagaataa acggatccgc tggctggagc cccatgaagc acaaaggctg attgatgaat
      661 gtccggagcc attaaagtct gttgttgaat ttgcactggc aacaggctta agacgctcga
      721 acatcatcaa ccttgaatgg caacaaatag atatgcagcg ccgggtggca tggataaacc
      781 cggaagtgag gtagcctgag tttaacggac actccttcct gaaatagaat ggcatcagaa
      841 ggagctaata atgagcagaa aaacccgacg ttactctaaa gagttcaaag ccgaagctgt
      901 cagaacggtt c
//
LOCUS       DAEEDS010000266          897 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEEDS010000266 DAEEDS010000000
VERSION     DAEEDS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.274"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..155)
                     /locus_tag="KLH32_004327"
     CDS             complement(<1..155)
                     /locus_tag="KLH32_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140880.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGT"
     gene            205..>897
                     /locus_tag="KLH32_004328"
     CDS             205..>897
                     /locus_tag="KLH32_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0140881.1"
                     /translation="MTHITLADNFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPR
                     AFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGER
                     ADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNK
                     VLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNNSQVSCKGSV
                     DSTITDLLEKALN"
BASE COUNT          299 a          149 c          205 g          244 t
ORIGIN      
        1 gtaccgggct gagaggcggt gtaagtgaac tgtagttgcc atgttttacg gcaatgagag
       61 cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg
      121 gagcaggaag gacatctgat ggaaatggaa gccacgcaag caccttaaaa tcaccatcat
      181 acactaaatc agtaagttgg cagcattacc cacattacat tagccgacaa ttttaaacaa
      241 aaaaatgaag ccttagatac ctggtatgtg ggaatgaatg attttgcccg aattgccggg
      301 gggcagaata gcagaagcaa tattctttct cctagagcat ttttggagtt tttggcaaag
      361 atatttaccc tgggttatgt ggattttagc aaacgctcca acgaagcggg tagaaatatg
      421 atggctcata ttgagttctc atcttattct aaagatactg atggcaatga aaaaatgaag
      481 ttttacatga ataatcctga aggggagcga gcggatttat ctaaggtgaa gatagaaatt
      541 acacttgcct cagcttctac taaagggatt cgtgaaggac atacagtcat tatatttaaa
      601 caatctgatg gttcgactaa tcgttatgaa gggaagtctt ttgaaagaaa agatgatagt
      661 tcattacacc tgattactaa caaggttctg gcgtgttacc aacgcgaagc taacaagaaa
      721 atagcgcgtc tattaaataa tcatcagaag ttaaataata tacaggagtt aaatgattcg
      781 caggagttaa ataattcaca gaagttaaat aattcgcagg agttaaataa ctcgcaggtg
      841 agttgtaaag gttcagttga ttctacgatt acggatttat tagaaaaagc attgaat
//
LOCUS       DAEEDS010000267          833 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEEDS010000267 DAEEDS010000000
VERSION     DAEEDS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.275"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..326
                     /locus_tag="KLH32_004329"
     CDS             <1..326
                     /locus_tag="KLH32_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBE0140882.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="KLH32_004330"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="KLH32_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBE0140883.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          155 g          242 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga gtg
//
LOCUS       DAEEDS010000268          816 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEEDS010000268 DAEEDS010000000
VERSION     DAEEDS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.276"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>816
                     /locus_tag="KLH32_004331"
     CDS             <1..>816
                     /locus_tag="KLH32_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE0140884.1"
                     /translation="VARSEGVLVGYLPGLAMRRIADLHAGEAKTDARDAAIIAEAART
                     LPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPAPERVLGPRL
                     EHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPG
                     TNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLT
                     EVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAA"
BASE COUNT          158 a          278 c          219 g          161 t
ORIGIN      
        1 gttgcccgct cagaaggagt ccttgtcgga tacctccctg gactggccat gcgccgcata
       61 gccgacttac acgccggtga agctaaaact gatgctcgtg acgctgccat cattgccgaa
      121 gctgcccgta ccctgcctca cgcgctacgc acgctgaaac tggctgacga gcaaatcgcc
      181 gaactctcca tgctctgcgg cttcgatgat gatcttgccg cacagacaac gcaggccagc
      241 aaccgtatcc gcggccttct gacccagata catccggcac cggagcgcgt tctcggtccg
      301 agacttgagc acccggcggt actcgatctt ctccagcgat atccctcacc agaaaaactc
      361 gcttcgctgg gtgagaagaa gctggcagcc cagctctgca aacttgcgcc tcgtctgggt
      421 aaacgccttg cagcagacat agctcaggca ctggccgaac aaaccgtcgt cgttcccggc
      481 acgaatgccg ctgccgtagt actgccacgt ctggcactcc agctcatcac gctgcgtaag
      541 caaagagacg aggtggcgct tgaggtagaa cagcgagttc ttgctcaccc tctttacccg
      601 gtcctgacca gtatgcccgg agtcggtgtc aggaccgcag ccagactcct caccgaggtc
      661 gcctgccgcg ccttcgcctc tgccgcacat ctcgctgctt atgctggcct tgcgccggta
      721 actcggcgat ccggctcgtc aatacgcggt gagcatccct cgcgacgggg taataaagct
      781 ctcaaacggg cgttgttcct gtcggccttc gccgcg
//
LOCUS       DAEEDS010000269          812 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEEDS010000269 DAEEDS010000000
VERSION     DAEEDS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.277"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..366)
                     /locus_tag="KLH32_004332"
     CDS             complement(<1..366)
                     /locus_tag="KLH32_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0140885.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEG"
     gene            complement(378..779)
                     /locus_tag="KLH32_004333"
     CDS             complement(378..779)
                     /locus_tag="KLH32_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBE0140886.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          200 a          252 c          168 g          192 t
ORIGIN      
        1 cccttcataa tatcgtcctg cccttacgtt tcccggcagc ccctcacgcc gcaaccggat
       61 ataactacat ccgccatcat caggggtgat acgatccaca cagaaagact cacgaccaat
      121 catcagcgaa tcattgcgac acagccgtaa aatatctgct gttttcacaa acagggatgg
      181 taaagagtct tcaatacgca caccgccggc caccagagaa atattttcag gatcatcaaa
      241 aatcccggaa atcatgatcc cttttagccg gcctgacgta atgaccgctc taatccccat
      301 attgcgaata atggccttgt ctgcaagatc aagtgcaaca tcaaacagat tatcgaaatc
      361 agccacgctg cctcccgtta ttgcattctg gccaggccac gttctgtcat tccggctgcc
      421 acaccggcaa agacacggaa cgccattccc ggcggcacaa atgccagagg ttcatcccgc
      481 gtggcatgaa gtgcatcggt atgcagcgtc accagtgcca cgactgtaac cagatcatcc
      541 atattctgaa tcacggtctc cggctgcgct gatatcacct cattttcatg tccggtcagc
      601 gcattttccg ggtgggcaga cttatcccgg atgtcagtgt catccatatt ggcaagttcc
      661 tcctccagct cagccacacg ctgcgcaata tctgctgcgc ttcccgagat atccggctca
      721 cgtcccagcg atgctgccag ttctctcaga cgtttcaggt tatcgtcttt ctttgccata
      781 tctgttcctg tccgaataaa aaacgggagc cc
//
LOCUS       DAEEDS010000270          811 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEEDS010000270 DAEEDS010000000
VERSION     DAEEDS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.278"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>811
                     /locus_tag="KLH32_004334"
     CDS             <1..>811
                     /locus_tag="KLH32_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBE0140887.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAEEDS010000271          789 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEEDS010000271 DAEEDS010000000
VERSION     DAEEDS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.279"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..789
                     /locus_tag="KLH32_004335"
                     /pseudo
     CDS             1..789
                     /locus_tag="KLH32_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          241 a          145 c          174 g          229 t
ORIGIN      
        1 aataaacaaa tcaccgctaa aggtggctgg aaagctattt cagaaagtac acaggcacaa
       61 ctagggaact tgaaaaactc ttggaatacc acgcttgata gtatcttcag aggttctgaa
      121 aatggtctgc aagattttac aagaagctta accaatcttt taaacgctct tggtggaagt
      181 ggtaagagtc ttggtgaatc tcttggtagt ctcatgactt ctatgtcaca tggtgtcgat
      241 tcattgaccg atattagtta cagggtaagg gcgttctttg atgaggtgac attagcttac
      301 cgtccactaa atgacactca gaggaaaatt gttgatggtt tatctggtgg gttaattgat
      361 ggtctgaaga tgcttgctgt ggctgttact gctcaaaagg ctgcaaatgt agcaggaggc
      421 ttgttaaacc ttgcaaacgc tgttacttca ttcggtgacg ctgttaataa agctaaagct
      481 ggcaaggctg gttcttcagg tggtgcgcta aataaagtcg gtagagttgt cctttatgct
      541 gagttggtaa attttgctct tgataattta aaccctgaag cagctaagaa tccgaactta
      601 catcaatcag gtattggcac agaaacaaac ttcaaccctg aatacaaagg tgattcatct
      661 aagccaatag ttctatcgct gactggttta atggtgcaac taataaaatt gctaacttcc
      721 aagaaccgat gaataacttc gtaaataaca cacaatcaac tccatcagct atcacaggtg
      781 acatggtta
//
LOCUS       DAEEDS010000272          782 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEEDS010000272 DAEEDS010000000
VERSION     DAEEDS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.280"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>782
                     /locus_tag="KLH32_004336"
     CDS             <1..>782
                     /locus_tag="KLH32_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HBE0140888.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLV
                     KLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDA"
BASE COUNT          194 a          224 c          220 g          144 t
ORIGIN      
        1 ccttgccgaa tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt
       61 ctggtgtatt gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg
      121 cctggacatc atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc
      181 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
      241 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      301 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      361 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      421 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      481 acttatagaa caaaccggcg ataacactct gacgttaatg gataaaggtt attactcact
      541 gggactgtta aatggctgga gcctggcggg agaacaccgc cactggatga tacctctcag
      601 aaagggagcg caatatgaag agctcagaaa actgggtaaa ggcgatcatc tggtgaagct
      661 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg
      721 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      781 ca
//
LOCUS       DAEEDS010000273          755 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEEDS010000273 DAEEDS010000000
VERSION     DAEEDS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.281"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(137..754)
                     /locus_tag="KLH32_004337"
     CDS             complement(137..754)
                     /locus_tag="KLH32_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE0140889.1"
                     /translation="MSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDR
                     DQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRT
                     EQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSMPE
                     LEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          146 a          239 c          179 g          191 t
ORIGIN      
        1 gaggcccgcc cccgggcctc cccggattca tccgtttctc tgttcagcct gaccgcacac
       61 cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag cggtcttctg tgattccgcc
      121 agttgcggct tattcattac ttaacgtcaa acgcccgaat tgaagccaaa tcatccagac
      181 tgctcagctc ctctttcatc tcccgctgac ggcgataaat ctcatcgttg cgatcgacct
      241 gcgcctgcac cattgctgcc gccagttctt ccagttccgg catcgacagt ttcacctgct
      301 gattatccgc atcactccac accatatgtg tttgtgccgc gacagatttt gccagcatga
      361 ccaccgggga caggcgggca actgacgagg cgtcagcgtt ccacgtgcgg ccgttccatt
      421 caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc ttcaatttcc tgacgtctgg
      481 cctctctggc cgcttccagc atttctggtg tcacagtgaa tggggctatc tcaccccatt
      541 tgccactttg cagttcctgc cagatttgct gacccgtcgg tgcaacatca tcagcggtgg
      601 ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac gtcacagtct actgcgccac
      661 tttcggtata acggggatta atgatttttt taatttccac ggtgcattcc tcacgatgtg
      721 cgaataaaaa gcccgggcat tgcgccagag acatg
//
LOCUS       DAEEDS010000274          747 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEEDS010000274 DAEEDS010000000
VERSION     DAEEDS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.282"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>747
                     /locus_tag="KLH32_004338"
     CDS             <1..>747
                     /locus_tag="KLH32_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBE0140890.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DAEEDS010000275          714 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEEDS010000275 DAEEDS010000000
VERSION     DAEEDS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.283"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            60..400
                     /locus_tag="KLH32_004339"
                     /pseudo
     CDS             60..400
                     /locus_tag="KLH32_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            475..>714
                     /locus_tag="KLH32_004340"
     CDS             475..>714
                     /locus_tag="KLH32_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0140891.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELEREN"
BASE COUNT          219 a          161 c          185 g          149 t
ORIGIN      
        1 ttgttctgag gtgagcatat cacctctgtt caggtggcca aattcagtgt gccacttcaa
       61 acagtgaatt atttgccaac gacggtacac cgacatggct aaaagaaaag cgtgcaaaag
      121 aaatcgcact tgatgaaatt aacttgatga aattatcgag ttgctcaaca agcaccatgg
      181 gggcaccagt aacgacgcca aacgcgcaaa agctgatttg ctggaaaaaa cattcacctc
      241 ccgctcatgg gaaagaatca aaggtatgga ctggccgaca atcaaagctg gtcgtaatgc
      301 actatggatt gaactggaag gcgttgaata tgcattcccg gatccacaaa cacaaaacga
      361 aacgcaacaa gcaggatacg atgagaatat tccggtgtaa aaccaagtgc attttgggaa
      421 tgaaccgccc cgggaatcct ggagactaaa cttcctgaga aagaggtaaa caggatgact
      481 aaaaatactc gtttttcccc cgaagtccgt caacgggcag tccgtatggt tctggaaagt
      541 cagggcgaat atgactcaca atgggcgaca atttgttcca ttgccccaaa gattggctgt
      601 acgccggaga ctctgcgtgt ctgggttcgc cagcatgagc gggataccgg gggcggtgat
      661 ggagggctca ccaccgctga acgtcagcgt ctgaaagagc tggagcgtga aaat
//
LOCUS       DAEEDS010000276          701 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEEDS010000276 DAEEDS010000000
VERSION     DAEEDS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.284"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            62..607
                     /locus_tag="KLH32_004341"
                     /pseudo
     CDS             62..607
                     /locus_tag="KLH32_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          175 a          176 c          179 g          171 t
ORIGIN      
        1 acgaatcgga aaatcgatac tggaaaaact ctaactcggt ggccagtttt tgttgaccac
       61 ttcaggtcag gagattgtcg tcgaaccgat tgaacaggct attctcatct ctgagcagac
      121 taagctgtgg gacgccaagt catccaccgg attcactcag ggcacacttc agcaggacgc
      181 tatgaacatc tgtgagaatg tggtccgtac catcaacgct cgcatggtcg acgttctgga
      241 atcctctaag ctgctgaaga ccgtagagct tcctgtcctg actggtagcc tgaccgctaa
      301 ggcagacgct atcatggacg ctctgtatga gaacaccgag ccttctttcg gttctgaggt
      361 gtctgactac ggtattatcg cccatgagag ccaactgaaa gcactgtccc gtctggctgc
      421 taaacagggc ttctctggtg aagacgctat cgtggatatg ctgggtaccg acatcgcgta
      481 ttacaacggt gaggacaaag gtgtgttcat gttggctaaa cgcttcacgg ccctgagctt
      541 cggctgcttc cgtcacgacg gtgagaatat tacggtcgtc ctttcacgtg atggcgactc
      601 tcagtcctga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatcaga
      661 aggagctaat aatgagcaga aaaacccaac gttactctaa a
//
LOCUS       DAEEDS010000277          693 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEEDS010000277 DAEEDS010000000
VERSION     DAEEDS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.285"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..525
                     /locus_tag="KLH32_004342"
                     /pseudo
     CDS             1..525
                     /locus_tag="KLH32_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>693
                     /locus_tag="KLH32_004343"
     CDS             589..>693
                     /locus_tag="KLH32_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0140892.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DAEEDS010000278          692 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEEDS010000278 DAEEDS010000000
VERSION     DAEEDS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.286"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..490
                     /locus_tag="KLH32_004344"
     CDS             <1..490
                     /locus_tag="KLH32_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0140893.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EM"
     gene            552..>692
                     /locus_tag="KLH32_004345"
     CDS             552..>692
                     /locus_tag="KLH32_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0140894.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRLFLPEGTLGQW
                     VTA"
BASE COUNT          174 a          153 c          170 g          195 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatcaga
      541 aggagctaat aatgagcaga aaaacccgac gttactctaa agagttcaaa gccgaagctg
      601 tcagaacggt tcttgaaaat caactttcga tcagtgaaga cgcttcccga ttattccttc
      661 ctgaaggcac tttaggacaa tgggttaccg cc
//
LOCUS       DAEEDS010000279          687 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEEDS010000279 DAEEDS010000000
VERSION     DAEEDS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.287"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..667)
                     /locus_tag="KLH32_004346"
     CDS             complement(<1..667)
                     /locus_tag="KLH32_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBE0140895.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          185 a          173 c          154 g          175 t
ORIGIN      
        1 ttgccgcatc atcatcagac tccggaacgc cctcatacac atgtttaaaa ccatccggat
       61 cacggcgtcg cgccgcgtcg ataaccttaa gcatggtgtc agacagaaac ggattttcgt
      121 catagttaat tttgcggatg agagtgcctt cgggcggatc aacaacgaag ttgcgccaga
      181 cgaaatcagt aacaagtccg gggttgaata tgaaccagca ttccgaaccc tctttacgga
      241 tcgttggctc cagaattttc cactggtatt ccgtcagtgc gtgggcttct tccagccaca
      301 acatatcgat cccctccagt gacttaattt cttcgatgtt gcgccataat ccataaaaaa
      361 caaattccga gccagtaacc cggttaatga ttttgttgtt cagaatacgg aaacggtgcc
      421 gcagaccaaa cctgtctatc tgaattttga gcagggtata caccgactct tcgattttat
      481 tctggatctg acgggcacaa caaaaacgca gggtgtattt attcgacaga aatatggcaa
      541 tgccagcggc atcccatgat tttgacgatg accgaccacc ataaagcact ttgttacgtg
      601 cccgcatagt ccagaaacta cgtaaagccg gattcagcgt cggtttggat gtcagagtag
      661 aagtcattga ggtcacgctc tccattg
//
LOCUS       DAEEDS010000280          665 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEEDS010000280 DAEEDS010000000
VERSION     DAEEDS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.288"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..665
                     /locus_tag="KLH32_004347"
                     /pseudo
     CDS             1..665
                     /locus_tag="KLH32_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          170 a          170 c          180 g          145 t
ORIGIN      
        1 ataaagaagt acggtccgga ttcttccact aataaagaac agtcagatgc tcaggccgag
       61 atccgccgtc tccagaaaga gctgaaacgg gttaccgacg aacgggacat attaaaaaaa
      121 gccgcggcgt acttcgcaaa gctgtccgac tgaggtacgc ctttatccgt gacaactcct
      181 gttgctggcc tgttcgcctg ctctgtcggg tgctggatgt tcatcccagt ggtttttacg
      241 cctggcttca gcagccgcat tcacaacgcc atcaggcaga cctgagactg acaggacaga
      301 ttaaacagtt ctggctggaa tcgggatgcg tctatggtta tcgcaaaatc catctggatc
      361 tgcgtgacag cgggcaacag tgcggagtaa acagagtctg gagactgatg aaacgtgtcg
      421 gaataaaggc tcaggtcgga taccgaagcc cgcgggcacg taaaggcgag gccagtatcg
      481 tgtcacccaa caggctccag cgacagttca atccggatgc tcctgatgag cgttgggtaa
      541 cggacataac ctacatcagg acccacgaag gctggctgta tcttgccgtt gttgttgatc
      601 tgttctcacg caaaattatc ggctggtcca tgcaatcccg gatgacaaag gacattgtcc
      661 tgaac
//
LOCUS       DAEEDS010000281          655 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEEDS010000281 DAEEDS010000000
VERSION     DAEEDS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.289"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..297
                     /locus_tag="KLH32_004348"
     CDS             <1..297
                     /locus_tag="KLH32_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBE0140896.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..569)
                     /locus_tag="KLH32_004349"
                     /pseudo
     CDS             complement(405..569)
                     /locus_tag="KLH32_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
BASE COUNT          211 a          151 c          120 g          173 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaatgat agactggccc cctgaatctc cagacaacca
      601 gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat tggag
//
LOCUS       DAEEDS010000282          636 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEEDS010000282 DAEEDS010000000
VERSION     DAEEDS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.290"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            88..153
                     /locus_tag="KLH32_004350"
                     /pseudo
     CDS             88..153
                     /locus_tag="KLH32_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="KLH32_004351"
     CDS             381..629
                     /locus_tag="KLH32_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE0140897.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAEEDS010000283          630 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEEDS010000283 DAEEDS010000000
VERSION     DAEEDS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.291"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..112)
                     /locus_tag="KLH32_004352"
     CDS             complement(<1..112)
                     /locus_tag="KLH32_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE0140898.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="KLH32_004353"
     CDS             complement(109..399)
                     /locus_tag="KLH32_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE0140899.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>630)
                     /locus_tag="KLH32_004354"
     CDS             complement(399..>630)
                     /locus_tag="KLH32_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0140900.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          149 a          181 c          136 g          164 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc acgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg
      301 gtaatgcgcc agcacagacg tttccggatc gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttaagtaa atccaccatt acgcaaactc cagcagctgc
      421 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacagaat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat attcgcaaac
      541 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
      601 ccggcctgaa tggctaccca ctcgcggaaa
//
LOCUS       DAEEDS010000284          628 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEEDS010000284 DAEEDS010000000
VERSION     DAEEDS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.292"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KLH32_004355"
     CDS             complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KLH32_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBE0140901.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(190..555)
                     /gene="csgA"
                     /locus_tag="KLH32_004356"
     CDS             complement(190..555)
                     /gene="csgA"
                     /locus_tag="KLH32_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBE0140902.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          164 a          142 c          150 g          172 t
ORIGIN      
        1 aatatttgta ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac
       61 atatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat
      121 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      181 aatgatgtat taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      241 gacggaggag ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      301 aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc
      361 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      421 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
      481 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tattattact
      541 gaggtgtact ggcaatagcg gacactacca tttgttcttt tttaagcagc catctgatga
      601 tatttttccc tgaaggctgc cggggaga
//
LOCUS       DAEEDS010000285          602 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEEDS010000285 DAEEDS010000000
VERSION     DAEEDS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.294"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..127
                     /locus_tag="KLH32_004357"
     CDS             <1..127
                     /locus_tag="KLH32_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBE0140903.1"
                     /translation="VTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(161..364)
                     /locus_tag="KLH32_004358"
                     /pseudo
     CDS             complement(161..364)
                     /locus_tag="KLH32_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            464..>602
                     /locus_tag="KLH32_004359"
     CDS             464..>602
                     /locus_tag="KLH32_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0140904.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          157 a          146 c          137 g          162 t
ORIGIN      
        1 ggtcacctgc aaacagaacg aagtggagcc ggaggactgg ttgcgcgaag tgatcgagaa
       61 gctcaatgac tggccgtcga accaagtgca tgaactgctg ccctggaact tctcgtctgt
      121 aaaataatcc ttacgctacg tacttctcgg ggcgcttacc tggagaataa cgtttatcca
      181 tattcttcca gtctggttca tcttccgacg tcatcaccgg gttgaaatta cgccgatata
      241 aatcgagttc cgtcacctgt attttccgct ccattgcccg ctgatggatc gcatcagcaa
      301 tatttgcggt caatgaatca tgacgaggat gggcccatac caaataaata cgttcagact
      361 gcataatttt acctccacgt taagttctgg agcgcacttt acgcaggcgg cataatcctg
      421 taaacgtgat gattcaggat gagttaatga aggattttca ctaatgcgga tgaatatgtc
      481 tgactttgcc acttcctttg ccgtggcccg taatcaaagc tttcgtgcag caggcgatga
      541 gttaggctta tcctcgtccg ccattagcca tagtattaaa acactggaac aacgtcttaa
      601 aa
//
LOCUS       DAEEDS010000286          590 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEEDS010000286 DAEEDS010000000
VERSION     DAEEDS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.295"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..293)
                     /locus_tag="KLH32_004360"
     CDS             complement(<1..293)
                     /locus_tag="KLH32_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBE0140905.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPETT"
     gene            complement(293..421)
                     /locus_tag="KLH32_004361"
                     /pseudo
     CDS             complement(293..421)
                     /locus_tag="KLH32_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            486..>590
                     /locus_tag="KLH32_004362"
     CDS             486..>590
                     /locus_tag="KLH32_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0140906.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
BASE COUNT          160 a          145 c          125 g          160 t
ORIGIN      
        1 gttgtttccg gtatatcgac tttgatgtac gggttaattt gtctggcgac agtgacaagg
       61 tctttattca ggagagcttt gcattctgct tcggtatacg ttttaccagg catgatgtct
      121 tttccggtgt gtccgtgaca tacagtccat acaccaatga tatctttgta tggtatgtag
      181 ctgacacctt ccagaccatc gttaccacct gggccagtga ttaacacaga tgctatagca
      241 acagccccgc caccaatagc aactgcaaca gccttgcgta atgatggcga cattattcac
      301 ctctcgcagc cttacgctta tcttctctta ttttgaaata cagattcgtc agataagtca
      361 gaagccccag aagcagactt cccagcacac caatcgcagc ccactgtgat ggactgacct
      421 gtgaggtagc ctgagtttaa cggacactcc ttcctgaaat agaatggcat cagaaggagc
      481 taataatgag cagaaaaacc caacgttact ctaaagagtt caaagccgaa gctgtcagaa
      541 cggttcttga aaatcaactt tcgatcagtg aaggcgcttc ccgattatct
//
LOCUS       DAEEDS010000287          586 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEEDS010000287 DAEEDS010000000
VERSION     DAEEDS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.296"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>586
                     /locus_tag="KLH32_004363"
     CDS             <1..>586
                     /locus_tag="KLH32_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBE0140907.1"
                     /translation="LAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          153 a          114 c          145 g          174 t
ORIGIN      
        1 gtttggcata tgctggtttg agttacaaag attttggttc attcgactac agtcgtaatg
       61 tcggtgtcgc gtatgatgca gaagcattta ctgatatgtt tgttgagtgg ggcggtgact
      121 catgggctgg tactgacctg ttcatgacca accgtaccaa cggtgttgca acctatcgta
      181 acacagattt ctttggcatg gttgaaggtc taaattttgc tctgcaatat cagggtaaaa
      241 atgaaggtac aggcaactac aaagcaaatg gcgatggcca tggtttatct gcaacttaca
      301 ctattgacgg tttctcattt gctggtgctt atgcaaactc tgaccgtact gattggcagt
      361 ctggtgatgg taaaggcgaa agagccgaag tttgggcgtt gtcaacaaaa tatgacgcta
      421 acaatgttta tgctgctgtg atgtacggtg aatctcacaa catgaattcc gacgacggtg
      481 atgttgtgaa caaaacacaa aacttcgaag ctgttctgca atatcagttc gactttggtc
      541 tgcgtccatc catcggttac tcctattcta aagcactgga cgtagc
//
LOCUS       DAEEDS010000288          554 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEEDS010000288 DAEEDS010000000
VERSION     DAEEDS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.297"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>554)
                     /gene="intI1"
                     /locus_tag="KLH32_004364"
     CDS             complement(<1..>554)
                     /gene="intI1"
                     /locus_tag="KLH32_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBE0140908.1"
                     /translation="VHWVRAFIRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQ
                     ALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQL
                     LYGTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARA
                     WWLKDQAEGRSGVALPDALERKYPR"
BASE COUNT          120 a          190 c          157 g           87 t
ORIGIN      
        1 cgcggatact tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg
       61 tccttcagcc accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc
      121 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
      181 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
      241 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
      301 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
      361 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
      421 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
      481 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
      541 cgaacccagt ggac
//
LOCUS       DAEEDS010000289          529 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEEDS010000289 DAEEDS010000000
VERSION     DAEEDS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.298"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..238
                     /locus_tag="KLH32_004365"
                     /pseudo
     CDS             1..238
                     /locus_tag="KLH32_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            338..>529
                     /locus_tag="KLH32_004366"
     CDS             338..>529
                     /locus_tag="KLH32_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0140909.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          181 a          109 c          104 g          135 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcatat tggctacgta attaaaacag
       61 aaaataaaga aaccccattc actgtagttg atatcgacgg tctatcagga aactttaaaa
      121 ctcttgttga aggtgccaca aaaatgtgcc tagtttacat cggaaataat ctgcccgcag
      181 aaaaaaagcc gaatttctgg caaatctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      241 aaaagaaagc ctgcacagtg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      301 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg agcacaaaac cactcttcct
      361 gttacggaaa gcgaaaaaat catccggtga acctgacgtc gtcctgtggg caagcgacga
      421 ttttgaatcg accagcacca ctctggacta tctgctcgtt aagtcaggta aaaaactgag
      481 caactatttt aaagctgttg ccacaaattt tcctgtcgtt aatgacctt
//
LOCUS       DAEEDS010000290          516 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEEDS010000290 DAEEDS010000000
VERSION     DAEEDS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.299"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            3..170
                     /gene="xisR"
                     /locus_tag="KLH32_004367"
     CDS             3..170
                     /gene="xisR"
                     /locus_tag="KLH32_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBE0140910.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..342
                     /locus_tag="KLH32_004368"
                     /pseudo
     CDS             172..342
                     /locus_tag="KLH32_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(359..>516)
                     /locus_tag="KLH32_004369"
     CDS             complement(359..>516)
                     /locus_tag="KLH32_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0140911.1"
                     /translation="ISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
BASE COUNT          150 a          103 c          123 g          140 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttccg gtgggtaatg actccaactt
      361 actgatagtg ttttatgttc agataatgcc cgatgacctt gtcatgcagc tccaccgatt
      421 ttgagaacga cagtgacttc cgtaccagcc ttgccagatg ttgtctcaga ttcagattat
      481 gtcgctcaat gcgctgagtg taacgcttgc tgataa
//
LOCUS       DAEEDS010000291          484 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEEDS010000291 DAEEDS010000000
VERSION     DAEEDS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.denovo.300"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>484)
                     /locus_tag="KLH32_004370"
     rRNA            complement(<1..>484)
                     /locus_tag="KLH32_004370"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          101 a          144 c          118 g          121 t
ORIGIN      
        1 ggtgtgtaca aggcccggga acgtattcac cgtggcattc tgatccacga ttactagcga
       61 ttccgacttc atggagtcga gttgcagact ccaatccgga ctacgacgca ctttatgagg
      121 tccgcttgct ctcgcgaggt cgcttctctt tgtatgcgcc attgtagcac gtgtgtagcc
      181 ctggtcgtaa gggccatgat gacttgacgt catccccacc ttcctccagt ttatcactgg
      241 cagtctcctt tgagttcccg gccggaccgc tggcaacaaa ggataagggt tgcgctcgtt
      301 gcgggactta acccaacatt tcacaacacg agctgacgac agccatgcag cacctgtctc
      361 acggttcccg aaggcacatt ctcatctctg aaaacttccg tggatgtcaa gaccaggtaa
      421 ggttcttcgc gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca
      481 attc
//
LOCUS       DAEEDS010000292        10801 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.117, whole genome shotgun sequence.
ACCESSION   DAEEDS010000292 DAEEDS010000000
VERSION     DAEEDS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.117"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..470)
                     /locus_tag="KLH32_004371"
                     /pseudo
     CDS             complement(1..470)
                     /locus_tag="KLH32_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314296.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            442..1227
                     /gene="lafU"
                     /locus_tag="KLH32_004372"
     CDS             442..1227
                     /gene="lafU"
                     /locus_tag="KLH32_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBE0140912.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            1298..2353
                     /gene="dinB"
                     /locus_tag="KLH32_004373"
     CDS             1298..2353
                     /gene="dinB"
                     /locus_tag="KLH32_004373"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBE0140913.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            2405..2698
                     /gene="yafN"
                     /locus_tag="KLH32_004374"
     CDS             2405..2698
                     /gene="yafN"
                     /locus_tag="KLH32_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBE0140914.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            2701..3099
                     /gene="yafO"
                     /locus_tag="KLH32_004375"
     CDS             2701..3099
                     /gene="yafO"
                     /locus_tag="KLH32_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBE0140915.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            3109..3561
                     /locus_tag="KLH32_004376"
     CDS             3109..3561
                     /locus_tag="KLH32_004376"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0140916.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            3807..4850
                     /locus_tag="KLH32_004377"
     CDS             3807..4850
                     /locus_tag="KLH32_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBE0140917.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            4912..5526
                     /locus_tag="KLH32_004378"
                     /pseudo
     CDS             4912..5526
                     /locus_tag="KLH32_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5583..7040)
                     /gene="pepD"
                     /locus_tag="KLH32_004379"
     CDS             complement(5583..7040)
                     /gene="pepD"
                     /locus_tag="KLH32_004379"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBE0140918.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7302..7760
                     /gene="gpt"
                     /locus_tag="KLH32_004380"
     CDS             7302..7760
                     /gene="gpt"
                     /locus_tag="KLH32_004380"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE0140919.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7852..9096
                     /gene="frsA"
                     /locus_tag="KLH32_004381"
     CDS             7852..9096
                     /gene="frsA"
                     /locus_tag="KLH32_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBE0140920.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            9154..9555
                     /gene="crl"
                     /locus_tag="KLH32_004382"
     CDS             9154..9555
                     /gene="crl"
                     /locus_tag="KLH32_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBE0140921.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(9594..10649)
                     /gene="phoE"
                     /locus_tag="KLH32_004383"
     CDS             complement(9594..10649)
                     /gene="phoE"
                     /locus_tag="KLH32_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBE0140922.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         2680 a         2622 c         2784 g         2715 t
ORIGIN      
        1 gggcttgcca gcaactggtg gcgcacgtca tgggcgatat cgccgttgtc gctgacgcgg
       61 gtgacgataa tcgccgccgc ggtggagagc agcagggaag ggatctgcgc caccaggccg
      121 tcgccgatgg tcatcagcac gtattgctgg aaggcggcgt cggcgctcag gttgtatttg
      181 aagatgccga tacagacgcc gccgctcagg ttgatcgcca gaatcatcat cccggcgatg
      241 gcgtctccgc gtacaaactt cgatgccccg tccatcgcgc cgtagaaatc ggcctcgctg
      301 gcaacatctt tacgccgggt ttgcgcctgc gcctggttga tcaatccggc gttaagatcg
      361 gcgtcaatcg ccatctgttt gccgggcatc gcgtccaggg taaagcgggc agaaacctcg
      421 gaaatacgct cggcctcttt ggtgacgacg ataaagttga ttgtcaacag cgtcagtaaa
      481 tccgagcgtg aaagcattat cgccgcgctg cacgggcagt cgatttttaa cggtggtggg
      541 ctgtcgccgt taaataaaat cagcccgtct catccgccaa aacctgccac cgtggcggtg
      601 ccggaagaga cggagaaaaa agcgcgtgat gtgaatgaga aaacggcgct gctgaagaag
      661 aaatccgcca ccgagcttgg cgagttggca accagtatca acaccattgc ccgtgatgcg
      721 catatggaag cgaatctgga gatggagatt gttccccagg gattacgcgt gctgattaaa
      781 gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc agattatgcc gttctttaaa
      841 actttgctgg tggagctcgc gccagtgttc gactcgctct ataacaaaat tattattacc
      901 gggcataccg atgcgatggc ctacaaaaac aatatctaca acaactggaa cctttcgggc
      961 gatcgcgcgc tttcagctcg tcgggtgctg gaagaggccg gaatgccgga agataaagtg
     1021 atgcaggtaa gcgcaatggc ggaccagatg ctgctggacg cgaagaatcc gcaaagcgcg
     1081 ggcaatcggc gtattgagat tatggtgctg accaaaagtg cgtccgatac gctgtatcaa
     1141 tactttggtc agcatgggga taaagtggtg cagccgctgg tgcaaaagct ggataagcag
     1201 caggtgcttt cgcagcgaat gcgctaaatg ctgaatcttt acgcatttct caaaacctga
     1261 aatcactgta tactttacca gtgttgagag gtgagcaatg cgtaaaatca ttcatgtgga
     1321 tatggactgc tttttcgcag cggtggagat gcgcgacaat cccgctctgc gcgatatccc
     1381 tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc agcaccgcca attatcccgc
     1441 gcgtaaattt ggcgtacgta gcgctatgcc tactgggatg gcgctcaaat tatgcccaca
     1501 tctcaccttg cttccggggc gctttgacgc ctacaaagaa gcctcaaatc atatccgtga
     1561 aatcttctcg cgctacacct cgcgcattga accgttgtca ctggatgagg cttatctcga
     1621 tgtcaccgat agcgtccatt gccacggttc tgcgaccctc atcgcccagg aaatccgcca
     1681 gacgatcttc aacgagctgc aactgacggc atctgcgggc gtggcaccag taaagtttct
     1741 cgccaaaatc gcctccgaca tgaataaacc caacggtcag tttgtgatta cgccggcaga
     1801 agttccggca tttttacaaa ccttaccact ggaaaaaatc cccggcgtcg gcaaagtctc
     1861 ggcggcaaaa ctggaagcga tggggctgcg gacctgcggt gatgtacaaa agtgtgatct
     1921 ggtgattctg cttaaacgct ttggcaaatt tggccgcatt ttgtgggagc gtagtcaggg
     1981 gattgacgag cgcgacgtta acagcgaacg gttacgaaaa tccgtcggcg tggaacgcac
     2041 gatggcggaa gatattcatc actggtctga atgtgaagcg attatcgagc ggctgtatcc
     2101 ggaacttgaa cgccgtctgg caaaggtaaa acctgattta ctgattgctc gccagggggt
     2161 gaaattaaag ttcgacgatt ttcagcagac cacccaggag cacgtctggc cgcggctgaa
     2221 taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat gaacgccgcg gcgggcgcgg
     2281 tgtgcgtctg gtggggctgc atgtgacgtt gcttgacccg caaatggaaa gacaactggt
     2341 gctgggatta tgatgtatac tattatgtat attctggtgt gcattattat gagggtatca
     2401 ctgtatgcat cgaattctcg ctgaaaaatc ggtcaatatc actgagttac gtaaaaaccc
     2461 agctaaatac tttattgatc aaccagttgc ggttctttct aataatcgcc ccgcaggata
     2521 tctcttaagt gccagcgcat tcgaagcgtt aatggacatg cttgctgaac aagaggagaa
     2581 aaagcccata aaggcgcgct ttcgtccaag tgctgcaaga ttagaggaaa ttacacgccg
     2641 cgctaaacaa tatcttaatg atatgacgga tgatgatttc aatgacttta aggaataagg
     2701 atgcgggtat tcaaaacaaa acttattcgc ctgcaactta cagcagagga acttgatgcg
     2761 ttaacggcgg attttatttc ctataagcgt gacggtgttt tgccagatat atttggtcgc
     2821 gatgcactct acgacgactc gtttacctgg ccattaatca aatttgagcg agttgctcat
     2881 attcatctgg caaatgtgaa taatccattt ccgccacagt tgcgccaatt cagcagaacg
     2941 aatgacgaag cgcatttggt atattgtcag ggtgcgtttg atgagcaagc atggttgctc
     3001 attgccattc tgaagcctga acctcataaa ctggctcgag ataacaacca aatgcataaa
     3061 attggaaaaa tggcagaagc gtttcgcatg cgtttttgaa tttatattat gaataacata
     3121 caaataagag attatcatcc tggcgatttt cagcaactat gcgctatttt cattagagcg
     3181 gttacgatga ccgtcagtca gcattattca ctacaacaaa ttgccgcctg ggcgcagatt
     3241 gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc agttattaat
     3301 gcaaaactgg tcggctttat tacctgcgtt gaacactata tcgatatgtt atttgttgac
     3361 cctgaataca cccgccgtgg ggttgccagc gctttgttaa aaccttttat taagtctgaa
     3421 tccgaactta cggtggacgc aagcataacc gcaaaaccct tttttgaacg ttatggtttt
     3481 cagacagtta agcagcagcg cgttgaatgc cggggagcgt ggtttattaa tttttatatg
     3541 cgttataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgc ctagtatgtg
     3601 cgcgttttct tattccacaa actgcaagga ggtaaatcat gacaaatcct ttatcaatga
     3661 ctctttgcag acctttccag gattaattct ttttttcttg ccctggattc gtctgccatt
     3721 tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc agcacatgca acttgaagta
     3781 tgacgagtct atttacggaa tggtatatgg gcaattatat tcgtccctta tccgatgcgg
     3841 tatttaccat cgcatctgat gacctgtgga tcgagagttt agcgatccaa caattacaca
     3901 ccacggcaaa tttacccaac atgcagcgcg tagtggggat gccagattta caccccggac
     3961 gcggctaccc gattggcgca gcgttcttct ccgcaggccg tttttaccca gcactggtcg
     4021 gcaatgatat cggctgtggc atggcgctat ggcaaacaga tattctcgct cgcaaataca
     4081 acgccgataa gtttgaaaag cgattatcag ctttggatga cgttgctgaa gaaagctggc
     4141 tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg cttggctcta
     4201 tcggtggtgg caatcatttc gctgaactgc aacaagttga tcaaatcatc aacgctgaac
     4261 tgtttgcact ggcaggtctg gatgcgcagc atctgcaact gctggttcat agcggctcga
     4321 gggggctagg gcagtctatt ttacagcggc atattgcctc gttttcgcat catggtttgc
     4381 ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa tcgccacctg atcgctttgc
     4441 gcataatgca acaggttaag gccaccggaa gtccggttct ggatgtggcg cataactttg
     4501 ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca tcgtaaaggt gccacgcctg
     4561 atgactgtgg tctggtggtt attcccggtt cacgcggtga ttactccagg ctggttcagc
     4621 ccgtcaggag tgaggaaaca ttgcattcgc tggcgcatgg ggctgggcgt aaatgggggc
     4681 gcaccgagtg taaagggcgt ctggcagcga aatacacagc gacgcaactc tcgcgtactg
     4741 aacttggcag tcgggtaatt tgtcgcgata aacaactcat cttcgaagaa gcgccacaag
     4801 cctataaatc ggctgaaagt gtggtgcaat gtctgggcag gccggattaa ttattcctgt
     4861 cgcgcggctg cgtccggtgc taacgctcaa aaacagtgga gggaaaaaag gatgatcttg
     4921 ctataactct cctcagcgca ggggccggaa gaatgttgtc tcgcagtgaa aaaggcgctg
     4981 gacaggctaa ttaaagaagc tgcccgacaa gacgtcgcgg taacggtgct ggaaacagaa
     5041 acaggccgct actctgacac gctgcgttcg gcgctgattt ctctggatgg cgacaacgca
     5101 tgggcgttaa gcgaaagctg gtgtggcact attcagtgga tttgcccgag cccgtatcgc
     5161 ccacatcacg ggcgtaaaaa ctggtttctg ggcattgggc gttttaccgc tgatgagcag
     5221 gagcaatccg atgcgatccg gtatgagacg ctgcgttcgt cggggccggg cggtcaacat
     5281 gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat tagcgtgaag
     5341 gttcagtcag agcgcagcca gcatgctaac aaacggctgg cgcgattgct gattgcctgg
     5401 aagctggatc agcagcaata ggaaaatagc gcggtgctga aatcacagcg gcgaatgttc
     5461 catcaccaga ttgaacgtgg caacccgcga cgaacgttta cagggatggc ttttatcgaa
     5521 ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag caaatcaaat
     5581 aattacttcg ccggaatttc tttcagcagt tcagtcagca gtgtccagta atgacctacg
     5641 ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgatagttgg cccgatagaa
     5701 accatgtcca tttccggata cggttttttg aacagaccac attccaggcc cgcgtggata
     5761 atctggatgt tcggcgtctt gttgaacagg cgctgatagg tttcacgtac cagatgcatc
     5821 accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc ggttttcgcg
     5881 ccagccagtt tacccagcga atccagcatg ctcaccacgt agtctttacc gctgtcgatc
     5941 agtgaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac cacaccgacg
     6001 ttcagggagg tttcgaccac accttttgcc acgtcggaat tgcggatcac accgttcggg
     6061 gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc agctttatcg
     6121 ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag ctcgtttttc
     6181 aggatctcct gataggtatt caccagagat ttcagtgcgt cgactttatc agctgcgaca
     6241 gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc gttgaaatcg
     6301 ataaggcgca ggtccagttc ttctgcatga cccgccagga agcgcaccag cagtttgttg
     6361 gcattaccca ggccaacgtg gatttcaccg ccggagtgac cgcctttcag accttttaag
     6421 gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg caggttggag
     6481 gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc ggagtcggtg
     6541 ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc gtccataccg
     6601 gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc
     6661 agaaccgcca gtgcagaggc cataccaatg ccgttatccg cacccagcgt ggtgccgcgc
     6721 gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa gtcatgcacg
     6781 gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac cggtttacga
     6841 ttttccatac ctgcggtagc aggtttacga atcaggatat tacctacctg atcgcgttcg
     6901 acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg ctcttcatga
     6961 taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg ctgtggagat
     7021 aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa cagtattgca
     7081 ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga tatgtattga
     7141 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     7201 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     7261 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     7321 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     7381 aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggg gcgttactgg
     7441 cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca
     7501 accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta
     7561 ttgatgacct ggtggatacc ggtggtactg ctgttgcgat tcgtgaaatg tatccaaaag
     7621 cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg
     7681 ttgatatccc gcaagatacc tggatcgaac agccgtggga tatgggcgtc gtattcgtcc
     7741 cgccaatctc cggtcgctaa tcttttcaac gcctggcatt gccgggcgtt gttcttttta
     7801 acttcaggca ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag
     7861 gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta
     7921 gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga tggtaaaacc
     7981 atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg tattgaccca
     8041 cgtgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgctgaacg taccgacgat
     8101 gatttatacg ataccgtgat tggctaccgt ggtggcaact ggatttatga gtgggccacc
     8161 caggcgatgg tgtggcaaca aaaagcctgt acggaagaag atccgaaact cagtggtcga
     8221 cactggctgc atgcggctac gttgtacaac attgccgcct atcctcatct gaaaggagat
     8281 gacctggccg agcaagcgca ggctttatca aaccgcgcct atgaagaggc cgcccagcgt
     8341 ctgccgggca cgatgcggca gatggagttt accgtacccg gcggtgcgcc catcaccggc
     8401 tttttgcata tgccgaaagg cgatggcccg ttcccgacag tattaatgtg tggtggtctg
     8461 gatgcgatgc agacggacta ttacagcctg tatgaacgtt attttgcgcc gcgcggcatt
     8521 gcgatgctga ctattgatat gccgtcggtg ggcttttctt caaaatggaa gctcacccag
     8581 gactccagcc tgttgcatca gcacgtctta aaggcgctgc ctaacgtacc gtgggtggat
     8641 cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt gcgtctggca
     8701 taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt tcataccctg
     8761 ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt tctggcgagt
     8821 cgtttgggga tgcatgatgc ttccgacgaa gcgttacgcg tggagctgaa tcgctattca
     8881 ttaaaagtgc aaggattgct ggggcgtcgc tgcccaacgc caatgttatc aggctactgg
     8941 aagaacgatc cgttcagccc ggaagaggac tcacgcttaa tcacctcatc atctgctgac
     9001 ggtaaattat tagagatccc atttaacccg gtgtatcgga attttgacaa aggtcttcag
     9061 gaaatcaccg gctggatcga aaaacgcttg tgttaaaaat ttgctaaatt ttgccaattt
     9121 ggtaaaacag ttgcatcaca acaggagata gcaatgacgt taccgagtgg acacccgaag
     9181 agcagattga tcaaaaaatt taccgcacta ggcccgtata ttcgtgaagg taagtgcgaa
     9241 gataatcgat tctttttcga ttgtctggct gtatgcgtca acgtgaaacc ggcaccggaa
     9301 gtgcgtgaat tctggggctg gtggatggag ctggaagcgc aggaatcccg ttttacctac
     9361 agctaccagt ttggtctgtt cgataaagca ggcgactgga agagtgttcc ggtaaaagat
     9421 actgaagtgg ttgaacgact ggagcacacc ctgcgtgagt ttcacgagaa gctgcgtgaa
     9481 ctgctgacga cgctgaatct gaagctggaa ccggcgaatg attttcgtga tgaaccggtg
     9541 aagttaacgg cgtgagtgaa atgtgccgga tgcatcacat ccggcaatat tcattaaaac
     9601 tgatacgtca tcccaaccgc gacagtatca tcattattaa tattcagctt attatcgcta
     9661 tccagttggt tgattttata atcaacaaac gctgacatat ttttgttgaa ataatacgta
     9721 gcaccgacgt cgatataatt gaccagatct tcatcaccga tgccctcaat atctttccct
     9781 ttcgataaga cataacccag cgatggacgc agaccaaagt caaactggta ttgagcgacc
     9841 gcttcaaagt tctgtgtctt attggcaaag ccgccagtta ttggcgtcat tttgcgtgtt
     9901 tcagaataga aagttgccag ataaatatta ttggcatcgt atttcagacc tgttgcccat
     9961 gcttctgcac gcttgcctgt gccacggctt tgcaggttct gctcgttggt gcgatctgag
    10021 ttggtatagg ccccactaat ggcgaaatcg ctgccgccaa agtcatatgt caatgacgtg
    10081 ccgaagccat cgccgttttg ctttttaacg tcgcggtttt cgtttttccc ttgatattgc
    10141 agggttaagt tcaggccatc gataacgccg aagaagtcgg tgttccgata cgtcgccaga
    10201 ccgctggcgc gtttggtcat aaagttgtcg gtctgcgccg aggaatcgcc accaaattcc
    10261 gggaacatat cggtccaggc ttccacgtca tacaacgcgc ccaggttacg accataatcg
    10321 aaagaaccca aatctttata tttcaacccg gcaaaagcga gacgcgtttt ttgctgtgca
    10381 gtatcactct ccgctttatt tccggcaaac tccgcttccc accgaccata accagtcagt
    10441 tgatcgttaa tttgagtttc acctttaaaa ccaaaacgga tataactctg gtcgccatct
    10501 ttactggcgt tatcactcat ataatgcatg gctttaactt tgccatagac atccagttta
    10561 ttaccgtctt tattatatat ttctgcagcc tgtacagatg cagatgccac aatgcccatc
    10621 accactaatg ccagagtgct ctttttcatt ttcattcctg attttaatta acgcgcgaat
    10681 attcagcggg agagccccgt tgaaaacagg aaagttttta acctgagatt gttaaagata
    10741 tattacagat taatgatatt ctgaaaatgg gtaatgctgc caacttactg atttagtgta
    10801 t
//
LOCUS       DAEEDS010000293         9721 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.126, whole genome shotgun sequence.
ACCESSION   DAEEDS010000293 DAEEDS010000000
VERSION     DAEEDS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.126"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(16..204)
                     /locus_tag="KLH32_004384"
     CDS             complement(16..204)
                     /locus_tag="KLH32_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE0140923.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="KLH32_004385"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="KLH32_004385"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBE0140924.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="KLH32_004386"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="KLH32_004386"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBE0140925.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="KLH32_004387"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="KLH32_004387"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBE0140926.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="KLH32_004388"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="KLH32_004388"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBE0140927.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="KLH32_004389"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="KLH32_004389"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBE0140928.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5099..6232
                     /locus_tag="KLH32_004390"
     CDS             5099..6232
                     /locus_tag="KLH32_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE0140929.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="KLH32_004391"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="KLH32_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE0140930.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="KLH32_004392"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="KLH32_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBE0140931.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="KLH32_004393"
     CDS             complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="KLH32_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBE0140932.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="KLH32_004394"
     CDS             complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="KLH32_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBE0140933.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7778..8344)
                     /gene="efp"
                     /locus_tag="KLH32_004395"
     CDS             complement(7778..8344)
                     /gene="efp"
                     /locus_tag="KLH32_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE0140934.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8386..9414
                     /gene="epmB"
                     /locus_tag="KLH32_004396"
     CDS             8386..9414
                     /gene="epmB"
                     /locus_tag="KLH32_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBE0140935.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2476 a         2486 c         2472 g         2287 t
ORIGIN      
        1 gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accaactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg cagatccgtg
     2281 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatcc
     2641 ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     7081 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     7321 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     7381 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7441 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7501 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7561 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga
     7621 tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga
     7681 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7741 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7801 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7861 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7921 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7981 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcattgt
     8041 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     8101 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     8161 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     8221 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8281 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8341 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8401 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8461 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8521 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8581 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8641 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     8701 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8761 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     8821 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8881 gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact ggaagccatc
     8941 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     9001 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     9061 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     9121 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     9181 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     9241 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9301 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9361 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9421 aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
     9481 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     9541 ccggcaagag caacaaacgc aagtacagca acctgtcatt aaactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt cttttcggta atgactccaa cttactgata gtgttttatg
     9721 t
//
LOCUS       DAEEDS010000294         5950 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.164, whole genome shotgun sequence.
ACCESSION   DAEEDS010000294 DAEEDS010000000
VERSION     DAEEDS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.164"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            52..711
                     /locus_tag="KLH32_004397"
     CDS             52..711
                     /locus_tag="KLH32_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBE0140936.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(912..1289)
                     /locus_tag="KLH32_004398"
     CDS             complement(912..1289)
                     /locus_tag="KLH32_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0140937.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1356..4322)
                     /locus_tag="KLH32_004399"
     CDS             complement(1356..4322)
                     /locus_tag="KLH32_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBE0140938.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4325..4885)
                     /locus_tag="KLH32_004400"
     CDS             complement(4325..4885)
                     /locus_tag="KLH32_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0140939.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5011..5361)
                     /locus_tag="KLH32_004401"
     CDS             complement(5011..5361)
                     /locus_tag="KLH32_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBE0140940.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5564..>5950)
                     /gene="intI1"
                     /locus_tag="KLH32_004402"
     CDS             complement(5564..>5950)
                     /gene="intI1"
                     /locus_tag="KLH32_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBE0140941.1"
                     /translation="VALPDALERKYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMY
                     DQTFQRAFKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMI
                     YTHVLKVGGAGVRSPLDALPPLTSER"
BASE COUNT         1186 a         1663 c         1803 g         1298 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc aggaagctaa aatggagaaa
       61 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      121 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      181 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      241 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
      301 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      361 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      421 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
      481 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
      541 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
      601 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
      661 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa
      721 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg
      781 atgaatggca gaaattcgaa agcaaattcg acccggtcgt cggttcaggg cagggtcgtt
      841 aaatagccgc ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga
      901 ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa
      961 ttgacgatat gatcatttat tctgcctccc agagcctgat aaaaacggtt agcgccgggg
     1021 ttggattttt cagcgttcca gctaaggcta aggcattcct gttcaagcgc aagccgggct
     1081 ataaagcgca ttatcgcttt acccgtgccc ttatttcgat cgcactgaga aacatacagc
     1141 tctttaatat gcagctgacc gctgtatcgg ggcgagggat aaagaatatt gcaacatgcc
     1201 aggccagtaa tgttattgcc gcagcgcgct ctgatcacca gggtaccgga aagccggttg
     1261 aataactttt tacagagata atccttcatc aacgcttcct gaatgatacc ttcgccataa
     1321 taggggtcgt ctcagaaaac ggaaaataaa gcacgctaag ccggttgcag aggccgtagc
     1381 ggcctgaact tccccgcgcc gatcttggcg ctgctgcgcc ataggtaatc accggtcagg
     1441 ttgatgtgct cccagccgag tggcgacagg tactgcaata gcgagtcatc gacggcatga
     1501 ccattgccgc gcaacgcatg cgccgcacgc tccaggtaga ccgtgttcca cagcacgatg
     1561 gccgccgtca ccaggttgag gccgctggcc cggtagcgct gctgctcgaa actgcggtca
     1621 cggatttcac caaggcggtt gaagaacacg gcacgggcca gcgcattgcg cgcctcgccc
     1681 ttgttcagcc cggcatgcac gcggcggcgt agctcgacgc tttgcagcca gtcgaggatg
     1741 aacagcgtgc gctcgatgcg gcccaactcg cgcagcgcga cggccaagcc gttctggcgc
     1801 gggtagctgc cgagtttcct gagcatcagc gaggccgtca ccgtgccctg cttgatcgag
     1861 gtggccagcc gcaggatttc gtcccaatgg gcgcggacgt gcttgatgtt gagcgtgccg
     1921 ccgatcatcg gcttgagcgc gtcataggcg gcatcgccct tcgggatgta gagcttggtg
     1981 tcgcccaggt cgcggatgcg cggcgcgaag cggaagccca agaggtgcat cagggcgaag
     2041 acgtgatcgg tgaagcccgc cgtgtcggtg tagtgctcct cgatccgcag gtcggattcg
     2101 tggtacagca ggccgtcgag cacgtaggtt gagtcgcgca ggccgacatt gaccaccttg
     2161 gtgtggaatg gcgcgtattg gtcggagatg tgggtgtaga aagtccgtcc tgggctgctg
     2221 ccatattttg ggttgatgtg ccccgtgctc tttgccttgc tagcggttcg gaaattctgt
     2281 ccgtccgatg atgatgtggt gccatcgccc cagtgcccgg caaagggatg ccgaaactga
     2341 gcgttgacca gttcagccaa cgctgtcgag tacgtttcgt cgcgggtatg ccaggcttgc
     2401 agccaagcga gcttcgcgta ggtcgtgccg gggcaggact cggccatctt ggtcaggccc
     2461 aggttgatcg cgtcggccag gatcgtggtc aacaacaggt tcttgtcctt ggccagatcg
     2521 cccgatttca agtgcgtgaa gtgccgggtg aagcccgtcc actcatcgac ttcgagcagc
     2581 agttcggtga tcttgacgtg cggcaggacc atggctgtct ggtctatcag cgcctgcgcg
     2641 gtgtcgggca ccgccgcatc cagcggcgtg atcttcaagc ccgactcggt gatgatggca
     2701 tccggcaggt cgttggctgc cgccatgcgg ttgacggtgg caagttgtgc ttccagcagc
     2761 gtcagccgct catgcagata ttgttcgcag tcggtggcca cggccagcgg caattcgctg
     2821 gactgcttga ggctggtgaa cttctcgggc ggtaccaggt agtcctcgaa gtccttgaac
     2881 tggcgtgaac cctgcaccca gatgtcgccc gagcgcaggg agttcttcaa ctcggacagc
     2941 gcgcacagtt cgtagtagcg ccggtcgatg ccggcgtcgg tcatcaccag tttctgccag
     3001 cgcggcttga tgaagccggt cggtgcatcg gctggcagct tgcgggcgtt gtcggtgttc
     3061 atgccgcgca gcacctcaat ggcatcaagc acgtttttgg cggcgggcgc ggcccgcagc
     3121 ttgagcacgg caaggaattc cggtgcatag cggcgcaggg tggcgtagct ctcgccgatg
     3181 cgatgcagga aatcgaagtc atcgggttgc gcgagcttct gcgcctcggt gacgctctcg
     3241 gcaaaggaat cccaggacat gacggcctcg atggcggcaa acgcatcgcg gcctgattgc
     3301 ttggcgtcga tcagcgcctg accgatgcgc ccgtacagac gtaccttggc gttgatggcc
     3361 ttgcctgacg cctggaactg ctgctgatgc ttattcttgg cagcgttaaa cagcttaccc
     3421 aggatgcggt cgtgcaggtc gatgatttcg tcggtgacgg tggccatgcc ctcggtggcc
     3481 agcgccacga gagtggcgta gcgccgttgc ggctcgaatt tggccaggtc ggcgggtgtc
     3541 atctggccgc cctcgcgggc aatcttgagc aggcggttct ggtgaaccag ccgctcgatg
     3601 ccggtaggca gatcgagtgc ctgccatgcc ttgaggcgtt cgatgtgttc cagcatatgc
     3661 cgcgaatttg gcttggccgg agactggcgc aaccaagcca accaggtcgt cttgccgttg
     3721 tcccggcgct tgagcagatc gtcgaggcgg cggcgatgcg cgtccgccag tggttcggcc
     3781 aaggcgtcgt agatgcgccg gttagcacgg gtgatcgcct cggcactcgc ccgctcgacg
     3841 gcgttgaggg cgggcagaat gaccgactgc cgccgcaggt gcccgatcaa ggcgctggcc
     3901 agcacgatgc ctttgtcggt ttgcatcgcc agctcggtca gcatctggac ggcctgccgg
     3961 taatggctca tggtgaaggg ccggaaaccg aacacggttt gcagctcgct caggtgctcg
     4021 cgccgggtct gctcccgctg gccgtactcg ttccagcttt cgacgccgac cttgagctgg
     4081 tcggcgacca gcttcaacaa gggcgggaac ggtagttcat cgacgcccag gatgacgccg
     4141 ggaaagcgca ggtaacagag ctgcaccgcg aagcccagcc gattggctgg cccgcgccgc
     4201 tgtcggatga tcgagaggtc ggtatcgttg aatgtgtaat gtcggatcag gtcgtccttg
     4261 gagtccggca acgccagcag gctttcccgc tcggcggcgg acaggatgga acgacgtggc
     4321 atatttactg atccgttctc aagtattgat acagggtttc gcgactgatt ccgaattcac
     4381 gagcaagctt ggtcttttgc tcgccagcct cgacacgttg gcgcagttcg gcaatacgct
     4441 cagacgacag ggatttcttc ctgccacggt aagccccgcg ttgcttggcg agcgcaatac
     4501 cctcgcgctg acgctcgcgg atcagggcgc gctcgaactc ggcgaacgcg cccatcaccg
     4561 agagcatcag gttcgccatc ggagagtctt cgccagtaaa actgaggtgt tccttgacga
     4621 attcgatatg cacgccgcgt tgtgtcagcg tttgcacgat ccggcgcaaa tcatcgagat
     4681 tgcgcgccag gcgatccatg ctatgcacca ccacggtgtc gccggtgcgg gcgaagctta
     4741 tcagcgcttc cagttgcgga cgcttgacat ccttgccgga tgccttgtcg ctaaaagcgc
     4801 gatcaacctt gacgccttcc agttgccgtt ccgggttctg gtcgaaggtg ctgaccctga
     4861 tatacccaat gcgctgtcca gtcatggaat tccctgcaaa atgtcaggga agactctatg
     4921 accttcaacg agatatgtca ataaattcaa aattcaatcc tatcctgacg caatttacac
     4981 atggcatctg acatcaggtt agggtatgcc tcaacctgac ggcggcgaat cacaagcgtc
     5041 cggtttgacg ctggtttcgg tcgcagtgct ggcccgcgcc tggaagcgct gatagcactc
     5101 cagcccgcag aaatgctcca cgtactcggc cccttccggc gtgaaggcgg catcgagcgg
     5161 gatttccttg cagcacacgc agcaactggt gctcggttca ttggcgttca tggtggtgtt
     5221 cctccattgg ttgacgaagc cgacgaaggc cgccgccggc atcggcctgg cgaacaggaa
     5281 accctgcacc gtgtcgcaac ccgcctgccg caaccacgca aggcagtcgg gtgtttccac
     5341 accctcggct accacctcca ttgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
     5401 cgactgcact atagcagcgg aggggttgga tccatcaggc aacgacgggc tgctgccggc
     5461 catcagcgga cgcagggagg actttccgca accggccgtt cgatgcggca ccgatggcct
     5521 tcgcgcaggg gtagtgaatc cgccaggatt gacttgcgct gccctacctc tcactagtga
     5581 ggggcggcag cgcatcaagc ggtgagcgca ctccggcacc gccaactttc agcacatgcg
     5641 tgtaaatcat cgtcgtagag acgtcggaat ggccgagcag atcctgcacg gttcgaatgt
     5701 cgtaaccgct gcggagcaag gccgtcgcga acgagtggcg gagggtgtgc ggtgtggcgg
     5761 gcttcgtgat gcctgcttgt tctacggcac gtttgaaggc gcgctgaaag gtctggtcat
     5821 acatgtgatg gcgacgcacg acaccgctcc gtggatcggt cgaatgcgtg tgctgcgcaa
     5881 aaacccagaa ccacggccag gaatgcccgg cgcgcggata cttccgctca agggcgtcgg
     5941 gaagcgcaac
//
LOCUS       DAEEDS010000295         4778 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.191, whole genome shotgun sequence.
ACCESSION   DAEEDS010000295 DAEEDS010000000
VERSION     DAEEDS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.191"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(63..239)
                     /locus_tag="KLH32_004403"
     CDS             complement(63..239)
                     /locus_tag="KLH32_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBE0140942.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            569..1384
                     /gene="sul2"
                     /locus_tag="KLH32_004404"
     CDS             569..1384
                     /gene="sul2"
                     /locus_tag="KLH32_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBE0140943.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1445..2248
                     /locus_tag="KLH32_004405"
                     /note="aph(3'')-Ib"
     CDS             1445..2248
                     /locus_tag="KLH32_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBE0140944.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2248..3084
                     /locus_tag="KLH32_004406"
     CDS             2248..3084
                     /locus_tag="KLH32_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBE0140945.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3191..3595)
                     /locus_tag="KLH32_004407"
                     /pseudo
     CDS             complement(3191..3595)
                     /locus_tag="KLH32_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3805..4665)
                     /locus_tag="KLH32_004408"
     CDS             complement(3805..4665)
                     /locus_tag="KLH32_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBE0140946.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1064 a         1289 c         1254 g         1171 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc aggcgcggga ataaaatccc
       61 attcagacgc cggatagatt caagcaagcc aacttgtcgt caaaatcggt gttgcaaaaa
      121 cgggagtgac catagattcc gttttctgag gcgaccccca catagagacc ttcccggtca
      181 ttcactttgt agagtttgtc cctgggcttg agattgcgca gcttggtatc ggtcagcatg
      241 taccttgtcc ttcttcgtct ccgataccca ctctattgta aacaagacat ttttatcttt
      301 tatattcaat ggcttatttt cctgctaatt ggtaatacca tgaaaaatac catgctcaga
      361 aaaggcttaa caatattttg aaaaattgcc tactgagcgc tgccgcacag ctccataggc
      421 cgctttcctg gctttgcttc cagatgtatg ctattctgct cctgcagcta atggagcacc
      481 gcaaacaggt tactcgcctg gggattccct ttcgacccga gcatccgtat gagactcatg
      541 ctcgattatt attattatag aagcccccat gaataaatcg ctcatcattt tcggcatcgt
      601 caacataacc tcggacagtt tctccgatgg aggccggtat ctggcgccag acgcagccat
      661 tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg atcgacctcg gtccggcatc
      721 cagcaatccc gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta tcgcgccggt
      781 gctggacgcg ctcaaggcag atggcattcc cgtctcgctc gacagttatc aacccgcgac
      841 gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg gttttccaga
      901 cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta tgcattcggt
      961 gcaagacggg caggcagatc ggcgcgaggc acccgctggc gacatcatgg atcacattgc
     1021 ggcgttcttt gacgcgcgca tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct
     1081 tgtccttgat cccggcatgg ggttttttct gggggctgct cccgaaacct cgctctcggt
     1141 gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc
     1201 gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac
     1261 actcgctgca gagcttgccg ccgccgcagg tggagctgac ttcatccgca cacacgagcc
     1321 gcgccccttg cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg caagaattcg
     1381 ttaactgcac attcgggata tttctctata ttcgcgcttc atcagaaaac tgaaggaacc
     1441 tccattgaat cgaactaata ttttttttgg tgaatcgcat tctgactggt tgcctgtcag
     1501 aggcggagaa tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat
     1561 cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa
     1621 aggtcgaggt gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg
     1681 cttggtgata acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct
     1741 caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca
     1801 atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg
     1861 caatgccgtc aatcccgact tcttaccgga cgaggacaag agtacgccgc agctcgatct
     1921 tttggctcgt gtcgaacgag agctaccggt gcggctcgac caagagcgca ccgatatggt
     1981 tgtttgccat ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg
     2041 cacgggtctg atcgaccttg ggcggctcgg aacagcagat cgctatgccg atttggcact
     2101 catgattgct aacgccgaag agaactgggc agcgccagat gaagcagagc gcgccttcgc
     2161 tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg ccttctatct
     2221 gcgattggac cctctgactt ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg
     2281 cacgtttcgc aacctgttct cattgcggac accttttcca gcctcgtttg gaaagtttca
     2341 ttgccagacg ggactcctgc aatcgtcaag ggattgaaac ctatagaaga cattgctgat
     2401 gaactgcgcg gggccgacta tctggtatgg cgcaatggga ggggagcagt ccggttgctc
     2461 ggtcgtgaga acaatctgat gttgctcgaa tatgccgggg agcgaatgct ctctcacatc
     2521 gttgccgagc acggcgacta ccaggcgacc gaaattgcag cggaactaat ggcgaagctg
     2581 tatgccgcat ctgaggaacc cctgccttct gcccttctcc cgatccggga tcgctttgca
     2641 gctttgtttc agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac
     2701 gcggcgatta tagccgatca aatgatgagc aatgcctcgg aactgcgtgg gctacatggc
     2761 gatctgcatc atgaaaacat catgttctcc agtcgcggct ggctggtgat agatcccgtc
     2821 ggtctggtcg gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc ggctgacaga
     2881 gacgaccttt gtctcgatcc tagacgcatt gcacagatgg cggacgcatt ctctcgtgcg
     2941 ctggacgtcg atccgcgtcg cctgctcgac caggcgtacg cttatgggtg cctttccgca
     3001 gcttggaacg cggatggaga agaggagcaa cgcgatctag ctatcgcggc cgcgatcaag
     3061 caggtgcgac agacgtcata ctagatatca agcgacttct cctatcccct gggaacacat
     3121 caatctcacc ggagaatatc gctggccaaa gccttagcgt aggattccgc cccttcccgc
     3181 aaacgacccc aaacaggaaa cgcagctgaa acgggaagct caacacccac tgacgcatgg
     3241 gttgttcagg cagtacttca tcaaccagca aggcggcact ttcggccatc cgccgcgccc
     3301 cacagctcgg gcagaaaccg cgacgcttac agctgaaagc gaccaggtgc tcggcgtggc
     3361 aagactcgca gcgaacccgt agaaagccat gctccagccg cccgcattgg agaaattctt
     3421 caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga
     3481 atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct
     3541 ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc
     3601 gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaggg
     3661 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3721 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3781 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3841 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3901 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3961 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4021 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4081 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     4141 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4201 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4261 taagttggca gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4321 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4381 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcacc
     4441 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4501 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4561 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4621 cgcaaaaaag ggaataaggg cgacacgaaa atgttgaata ctcatactct tcctttttca
     4681 atattattga agcatttacc agggttattg tctcatgagc ggatacatat ttgaatgtag
     4741 gcactgttgc aaagttagcg atgaggcagc cttttgtc
//
LOCUS       DAEEDS010000296         3868 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.204, whole genome shotgun sequence.
ACCESSION   DAEEDS010000296 DAEEDS010000000
VERSION     DAEEDS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.204"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            53..439
                     /locus_tag="KLH32_004409"
     CDS             53..439
                     /locus_tag="KLH32_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBE0140947.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            447..1133
                     /locus_tag="KLH32_004410"
     CDS             447..1133
                     /locus_tag="KLH32_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE0140948.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1111..1734)
                     /gene="tetR(B)"
                     /locus_tag="KLH32_004411"
     CDS             complement(1111..1734)
                     /gene="tetR(B)"
                     /locus_tag="KLH32_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBE0140949.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1816..3021
                     /gene="tet(B)"
                     /locus_tag="KLH32_004412"
     CDS             1816..3021
                     /gene="tet(B)"
                     /locus_tag="KLH32_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBE0140950.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3134..3727)
                     /gene="tetC"
                     /locus_tag="KLH32_004413"
     CDS             complement(3134..3727)
                     /gene="tetC"
                     /locus_tag="KLH32_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBE0140951.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT         1074 a          705 c          817 g         1272 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aggacaagga taatgtttga
       61 tgttcacgtt gttttagata atcaaatagg acaattagca ttactaggaa aaacattagg
      121 taataaaggt attggattgg aagggggagg gatatttacg gttggtgatg aatgccatgc
      181 tcattttctt gttgaacaag gaaaggaagc taaaatagcg ctagagcaag ctggactgtt
      241 agtacttgcg atccggacac cattaattcg taagttaaaa caggaaaaac cgggggaact
      301 tggcgaaata gcacgagtat tggcggagaa taacattaat attttagtgc aatacagtga
      361 ccatgctaac caactgatat taataacgga caatgatagt atggctgcat ctgttacgct
      421 cccttgggca ataaagtgaa cttgcgatgg ctaatttaat acgaaaagag gttacctttg
      481 agtcctcaat agccgcgata ggggcggcta tgtctgacat ttcacgagtt aaaatactca
      541 gtgctttgat ggatgggcga gcttggacgg ccactgagct aagttctgtg gcgaatatat
      601 cagcttcaac ggcgagcagt catttatcta aattattaga ttgccagcta atcacagtag
      661 tagctcaagg caagcatcgt tattttcggc tagcaggaaa agatattgct gaattgatgg
      721 aaagtatgat ggggatctcc ttaaaccatg gcgtacatgc caaagtttcc acgccagtgc
      781 atttacgaaa agcacgtact tgctatgatc atttagctgg cgaagttgcc gttaagatct
      841 atgattccct ttgtcaacag caatggatca ctgaaaatgg ttcaatgatc acattaagtg
      901 gtattcaata ttttcatgaa atgggaattg acgttccttc caaacattca cgtaaaatct
      961 gttgtgcgtg tttagattgg agtgaacgcc gtttccattt aggtgggtac gttggagccg
     1021 cattattttc gctttatgaa tctaaagggt ggttaactcg acatcttggt taccgtgaag
     1081 ttaccatcac ggaaaaaggt tatgctgctt ttaagaccca ctttcacatt taagttgttt
     1141 ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg
     1201 atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc
     1261 cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg
     1321 ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa
     1381 ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac
     1441 ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa
     1501 aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat
     1561 ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg
     1621 ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc
     1681 attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag acatcattaa
     1741 ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc cctatcagtg
     1801 atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat tggtaattac gttactcgat
     1861 gccatgggga ttggccttat catgccagtc ttgccaacgt tattacgtga atttattgct
     1921 tcggaagata tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt aatgcaggtt
     1981 atctttgctc cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc agtgctgttg
     2041 ttgtcattaa taggcgcatc gctggattac ttattgctgg ctttttcaag tgcgctttgg
     2101 atgctgtatt taggccgttt gctttcaggg atcacaggag ctactggggc tgtcgcggca
     2161 tcggtcattg ccgataccac ctcagcttct caacgcgtga agtggttcgg ttggttaggg
     2221 gcaagttttg ggcttggttt aatagcgggg cctattattg gtggttttgc aggagagatt
     2281 tcaccgcata gtcccttttt tatcgctgcg ttgctaaata ttgtcgcttt ccttgtggtt
     2341 atgttttggt tccgtgaaac caaaaataca cgtgataata cagataccga agtaggggtt
     2401 gagacgcaat cgaattcggt atacatcact ttatttaaaa cgatgcccat tttgttgatt
     2461 atttattttt cagcgcaatt gataggccaa attcccgcaa cggtgtgggt gctatttacc
     2521 gaaaatcgtt ttggatggaa tagcatgatg gttggctttt cattagcggg tcttggtctt
     2581 ttacactcag tattccaagc ctttgtggca ggaagaatag ccactaaatg gggcgaaaaa
     2641 acggcagtac tgctcggatt tattgcagat agtagtgcat ttgccttttt agcgtttata
     2701 tctgaaggtt ggttagtttt ccctgtttta attttattgg ctggtggtgg gatcgcttta
     2761 cctgcattac agggagtgat gtctatccaa acaaagagtc atcagcaagg tgctttacag
     2821 ggattattgg tgagccttac caatgcaacc ggtgttattg gcccattact gtttgctgtt
     2881 atttataatc attcactacc aatttgggat ggctggattt ggattattgg tttagcgttt
     2941 tactgtatta ttatcctgct atcgatgacc ttcatgttaa cccctcaagc tcaggggagt
     3001 aaacaggaga caagtgctta gttatttcgt caccaaatga tgttattccg cgaaatataa
     3061 tgaccctctt gataacccaa gagggcattt tttacgataa agaagattta gcttcaaata
     3121 aaacctatct attttattta tctttcaagc tcaataaaaa gccgcggtaa atagcaataa
     3181 attggccttt tttatcggca agctctttta ggtttttcgc atgtattgcg atatgcataa
     3241 accagccatt gagtaagttt ttaagcacat catcatcata agctttaagt tggttctctt
     3301 ggatcaattt gctgacaatg gcgtttacct taccagtaat gtattcaagg ctaatttttt
     3361 caagttcatt ccaaccaatg ataggcatca cttcttggat agggataagg tttttattat
     3421 tatcaataat ataatcaaga taatgttcaa atatactttc taaggcagac caaccatttg
     3481 ttaaatcagt ttttgttgtg atgtaggcat caatcataat taattgctgc ttataacagg
     3541 cactgagtaa ttgtttttta tttttaaagt gatgataaaa ggcacctttg gtcaccaacg
     3601 cttttcccga gatctcatct attgaaacag cttgatagcc tttttcaaca aacaatattc
     3661 gtgctgagtt aaccagtgat tgataggtac tcttaaaatt ttcttgttga tgatttttat
     3721 tttccatgat agatttaaaa taacataccg tcagtatgtt tatggtatca tgatgatgtg
     3781 gtcgtgacaa tcttaagaac atttaggtta ttttatgtat attgaacagc attctcgcta
     3841 tcaaaataaa gctaataaca tccaatta
//
LOCUS       DAEEDS010000297         3311 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.209, whole genome shotgun sequence.
ACCESSION   DAEEDS010000297 DAEEDS010000000
VERSION     DAEEDS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.209"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            109..1014
                     /locus_tag="KLH32_004414"
     CDS             109..1014
                     /locus_tag="KLH32_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBE0140952.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..2249
                     /locus_tag="KLH32_004415"
     CDS             1011..2249
                     /locus_tag="KLH32_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0140953.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2249..2833
                     /locus_tag="KLH32_004416"
     CDS             2249..2833
                     /locus_tag="KLH32_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0140954.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
BASE COUNT          550 a         1020 c         1063 g          678 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt tatgcttgcg gccggcgcgt
     2101 ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc ggccgtgttt gcggggctcg
     2161 tggctctact ctcgctccgt cttcaatttg ctacaatggc ggcggcagcc acagcctccg
     2221 ccccaaccca tacagaaggt gaacactgat gccccgcccc aagctcaagt ccgatgacga
     2281 ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt cccatagagt tcacgctcag
     2341 cggagtagca aaggaggtgg ggctctcccg cgcagcgtta atccagcgct tcaccaaccg
     2401 cgatacgctg ctggtgagga tgatggagcg cggcgtcgag caggtgcggc attacctgaa
     2461 tgcgataccg ataggcgcag ggccgcaagg gctctgggaa tttttgcagg tgctcgttcg
     2521 gagcatgaac actcgcaacg acttctcggt gaactatctc atctcctggt acgagctcca
     2581 ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc gcggtggtgg aggggatccg
     2641 caagcgactg cccccaggtg ctcctgcggc agctgagttg ctcctgcact cggtcatcgc
     2701 tggcgcgacg atgcagtggg ccgtcgatcc ggatggtgag ctagctgatc atgtgctggc
     2761 tcagatcgct gccatcctgt gtttaatgtt tcccgaacac gacgatttcc aactcctcca
     2821 ggcacatgcg taaacggagg tgtgcagagt ccctgcggca ggcgacgaac acgaccgtcg
     2881 tcgattagta ccggtacggt cggtggtatc gaagtcttga tcaccactca ggtctacggc
     2941 ttacaaatgg tgaccatccc gatacttgcg tcagagcacc gggccgattc tttgacagtg
     3001 aatcactccc gtaaggttgt gccggtgtgg gtgtcccggg tcgagacgat actccgccaa
     3061 tgcgcccagc aaacaacctg gccatcgcag gtggtgggga gcggtgtggc ggatgagttg
     3121 gacaagttgg tgtagcagca cgagcacggc gagataacat cgcaggagtt cgacatgctc
     3181 aagagacagc tgattgcgaa tcgcgatgca gattcataac ccgattgcgg gttggcttca
     3241 ctccaccatc accgagcaga ctagcacggc gggctctgtt gcaaagattg gcggcagtca
     3301 gaggtaggct g
//
LOCUS       DAEEDS010000298         1962 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.237, whole genome shotgun sequence.
ACCESSION   DAEEDS010000298 DAEEDS010000000
VERSION     DAEEDS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.237"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            109..939
                     /locus_tag="KLH32_004417"
     CDS             109..939
                     /locus_tag="KLH32_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBE0140955.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1052..1843
                     /gene="aadA1"
                     /locus_tag="KLH32_004418"
     CDS             1052..1843
                     /gene="aadA1"
                     /locus_tag="KLH32_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE0140956.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          614 a          433 c          435 g          480 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat
       61 taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca
      121 atacatatca acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt
      181 gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt
      241 ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca
      301 acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg
      361 gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc
      421 tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa
      481 gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat
      541 ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc
      601 gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat
      661 cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa
      721 gatctggata atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga
      781 accttacaaa acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt
      841 tttgtgtccg cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag
      901 aaaaatgcga tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc
      961 ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc
     1021 tcggcgcccc ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc
     1081 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1141 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     1201 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     1261 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     1321 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     1381 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     1441 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     1501 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     1561 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1621 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1681 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1741 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1801 tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc
     1861 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     1921 tgcggtgatg ctgccaactt actgatttag tgtatgatgg tg
//
LOCUS       DAEEDS010000299         1016 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF180328
            SAMN17129051-rid12251283.guided.268, whole genome shotgun sequence.
ACCESSION   DAEEDS010000299 DAEEDS010000000
VERSION     DAEEDS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129051
            Sequence Read Archive: SRR13348987
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042430.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042430.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:42:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 371
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 163 of 371
            Pseudo Genes (incomplete)         :: 97 of 371
            Pseudo Genes (internal stop)      :: 144 of 371
            Pseudo Genes (multiple problems)  :: 32 of 371
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129051-rid12251283.guided.268"
                     /strain="SF180328"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            58..531
                     /locus_tag="KLH32_004419"
     CDS             58..531
                     /locus_tag="KLH32_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBE0140957.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            752..>1016
                     /gene="mobC"
                     /locus_tag="KLH32_004420"
     CDS             752..>1016
                     /gene="mobC"
                     /locus_tag="KLH32_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBE0140958.1"
                     /translation="MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLK
                     QEAIEKLAERIDQHLDHVSELFDKTWSRWHDCHFQKTTAPVDWA"
BASE COUNT          287 a          224 c          264 g          241 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacccaggat gagaaccttg
       61 aaagtatcat tgatggctgc gaaagcgaaa aacggcgtga ttggttgcgg tccagacata
      121 ccctggtccg cgaaagggga gcagctactt tttaaagcat tgacctacaa tcagtggctt
      181 ctggtgggtc gcaagacgtt tgaatctatg ggcgcactcc ccaataggaa atacgcggtc
      241 gttacccgct caggttggac atcaaatgat gacaatgtag ttgtatttca gtcaatcgaa
      301 gaggccatgg acaggctagc tgaattcacc ggtcacgtta tagtgtctgg tggcggagaa
      361 atttaccgag aaacattacc catggcctct acgctccact tatcgacgat cgacatcgag
      421 ccagaggggg atgttttctt cccgagtatt ccaaatacct tcgaagttgt ttttgagcaa
      481 cactttactt caaacattaa ctattgctat caaatttgga aaaagggtta acaaagctat
      541 gcaatcgacg gcaaaaagct tcgttcgctt cgcgcactac gccttttccg cgattgatag
      601 cgacgttatg tgaatattga aatgagcatt ccaaagaaag gaagtagaaa aattatcgtg
      661 ggcgaaaatg agtttttgtg gctcattagg tcaaaactca catattcgca ggactgttta
      721 ggtacagaaa tgactgcggt cgttgaaccg gatgccggtc ctcactgatg aagaagtgaa
      781 caccgtacgg gaatcgtgcc ggcagcttgg agctatcggc cggaacctca accaggtggc
      841 cagggccttg aacatcgagt tcagggaaag tgacaagctc aagcaagagg ccatcgaaaa
      901 actggccgaa cggatcgacc agcatttgga ccatgtgtct gagctgttcg ataagacctg
      961 gagccggtgg cacgattgtc attttcagaa gacgactgca ccagttgatt gggcgt
//
